LOCUS       DAOOJH010000001        86838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_294_length_86838_cov_9.924836, whole genome shotgun sequence.
ACCESSION   DAOOJH010000001 DAOOJH010000000
VERSION     DAOOJH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 86838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86838
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_86838_cov_9.924836"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="PLJ75_00005"
     CDS             complement(<1..1652)
                     /locus_tag="PLJ75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPD03633.1"
                     /translation="MNMGNIKKYSNKIVDRFDAALRRIMQKIGKAPDKPQKQKSYSHF
                     ILYGIIIILINLVGLTLYVRLDLTKNSVYSLSPISKAVVSTLEEPLTVKIFFNKDLPA
                     PYNTVYRYLQDLMVEYDNAGNRYFHYEFIDVEKQKDAAGDFGIYPVQIREIRNDQVKF
                     RNASMGLAIIHGDIIEKIDSITEPEGLEYRITTLIKKMNGKIDALLKIKDPITVTLYA
                     SSNLPIPGMQNLNEQVYAQVQKCNIRNYNKIQYRFVDPVNNPQALTIAQMYGLPTLKW
                     PQFTTMEGRAIPPGQGMVGIVVEHNNKFETVQILTRSIFGQYAIGDLSRLEDMLNAAI
                     DNLISVNPKVCYITGHGEKDINDEQNGAAQFRKMLSDMYDLVTVDITKDEIPDDVITI
                     IINGPRSMYSEYELFKIDQFLMRGGNAILLLDSYVEQQAEGMQMFQRPPQALPVITGL
                     EGMLAHYGIKIRKDIVMDKQCFIAQQRGLGELTIYFAPMIDDEGLSDDCEITKYLKKI
                     VFLKASSLEADEGLLKANKASVKYIVKSSPYSWLQQGAINFMP"
     gene            complement(1656..2381)
                     /locus_tag="PLJ75_00010"
     CDS             complement(1656..2381)
                     /locus_tag="PLJ75_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPD03634.1"
                     /translation="MQQFKDSMKGAQLVAKKELAGFFSTPIGYIVISVFLVIAGFLFF
                     STFFLYGQAELRAYFELLPLLFAFVIPAITMRLFAEERNTGSFEMLMTLPLSTVQIVT
                     GKILAATIFAGIMVLPTLFYAVSVAFVGSPDIGPIIGGFIGAIILAAAYASIGVFASS
                     LTRNQIVAFIIGFAICIVLSLITKFLYFVSPKLVSLFEYISADYHFQNIARGIIDTRN
                     IIYFASVIIISSMASIYQLEVRR"
     gene            complement(2394..3320)
                     /locus_tag="PLJ75_00015"
     CDS             complement(2394..3320)
                     /locus_tag="PLJ75_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013243311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD03635.1"
                     /translation="MINVQNLVKHYGDVKAVDGISFTIRKGEVTGLLGPNGAGKTTTL
                     RILTCYLQPTSGMVSIDDYLVDENPIEIKKRIGYLPESAPLYPDMMVYDFLQFIADIR
                     EINDVERITDVAIQCGITEVMHKNINELSKGYKQRVGLAQAILHDPEILILDEPTNGL
                     DPNQIIEIRDLIKELGREKTVILSTHILQEVEATCSRVIIIDKGVIVADDLTSELKSA
                     KGKDIRINILAGGASFDQLKTALCAIPGINNVNHIHDEEGLCHVQVITGSDIDARPAI
                     FNCIKDNGWVLYEMNREYRSLEHVFRELTVGA"
     gene            complement(3569..3925)
                     /locus_tag="PLJ75_00020"
     CDS             complement(3569..3925)
                     /locus_tag="PLJ75_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPD03636.1"
                     /translation="MDERTVYSEHRPWGFFEVLSDTETHKVKRITVYPHKRLSLQRHA
                     RRKEHWFFIHGRGVVTLDTDNIPVDVGKCVDIDFNVVHRVENTGDENVVYIEVQLGEY
                     FGEDDIERIEDDYGRI"
     gene            complement(3931..5016)
                     /gene="serC"
                     /locus_tag="PLJ75_00025"
     CDS             complement(3931..5016)
                     /gene="serC"
                     /locus_tag="PLJ75_00025"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPD03637.1"
                     /translation="MGRIFNFSAGPSILPVEVLEEASRQLVDYRNTGMSLIEMSHRGK
                     IYDEVHNEAIVLVREMLQVPDDYDILFIQGGATLQFGMVPMALLMADSSADFVITGTW
                     AKKAYQDARLIGNARIIWDGAGEKYRRIPSIDELSLHDDAVYVHICSNETIDGIQWQQ
                     LPDTGNTPLIVDMSSDIMSRAVDWNKVSMLFAGTQKNLAPAGMALVIMKKSLVEKARK
                     DIPAYLRYDLHAKENSLYNTPPTFTVWMTNLTLHWIKGIGGLPEIEKRRDRKAAMLYD
                     AIDNSGGYYTSPVDKNSRSAMNVVWHLPDEQLENKFVDDAEKEGLSGLKGHRSVGGLR
                     ASLYNAMPVEGVQTLVEFMHRFMEKHG"
     gene            5114..5800
                     /gene="rsmI"
                     /locus_tag="PLJ75_00030"
     CDS             5114..5800
                     /gene="rsmI"
                     /locus_tag="PLJ75_00030"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPD03638.1"
                     /translation="MTGKLYIIATPIGNLEDITLRALRILREDTDIVYCEDTRQTSKL
                     LQHYGISIPAQSLHAHSGKTKLEYIISQLLSGKNIAYCTDSGTPAISDPGGELVALAR
                     HNNIPVIPIPGASAVTALVSVCGFPSSSFYFAGFLSKKQGKRKKQLQQLGQLQAIVIV
                     YESPYRIVKLLYDIAEIMPQATILIGREMTKHFEEFIYFTAAEVESIIPSLHQKGEFV
                     IALYNEASKH"
     gene            5999..6292
                     /gene="flgM"
                     /locus_tag="PLJ75_00035"
     CDS             5999..6292
                     /gene="flgM"
                     /locus_tag="PLJ75_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HPD03639.1"
                     /translation="MVIDKIGNIKNIVEPKNSKSVGKTISTNKSDSVEISSEAKKAAE
                     QSKIAHVVKQTPDIRAERVREIKEMIANGTYKFDDDRIIEKVAEKIAMQLLHK"
     gene            6319..7545
                     /locus_tag="PLJ75_00040"
     CDS             6319..7545
                     /locus_tag="PLJ75_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPD03640.1"
                     /translation="MAGIIDKKAESLFRFFMSKTYGETIEGLWPDLYIPTAIYAQPDG
                     ISLLPSIVQLFGSRVLFITSTVDFNQYNHLFMSISRSLQSNNIPFIVYDEFPESNTDY
                     IDSAVYFARKTHCNLIIGLGGIDSINAAKTVALLTPNYLFAHELFSYPHLYEPIPFIS
                     IPTHPLFGFEITPCTIISDIHDGIKKAFFHNYIFPKAIIIDPELSVSVEEDTIAKYTL
                     GTLAIATESVISKNINNITNTLSLRTIDLVFKNLHVALQESDNETAKLNLLMASLMAG
                     IAFSSAQLSVSLAISLALASLTQIDIASYMSVMLPHIMEYNLTSAPGKYVQMSKVMDE
                     DVRDITVIEAAIKAVEGVRKLALDIDIPQRLSQFDVNKTIFSKVAEITLSYPFIQNAP
                     RVLNRDEVETILIAAY"
     gene            7549..8310
                     /gene="larB"
                     /locus_tag="PLJ75_00045"
     CDS             7549..8310
                     /gene="larB"
                     /locus_tag="PLJ75_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPD03641.1"
                     /translation="MNYRPIKKLLQQFKDDTITMDDFIRELEHLNVEDLEHTTLDHQR
                     KARLGFPEVIFCQNKTPQQVADIAGAIAKQNYSFLATRASLAHFKAVKRYIKKAQYNT
                     LGKTISFSAQTMHHKPHRLLIITAGTSDIPVAEEAKETALVMGLTVETIYDIGVAGIH
                     RVFHNWDKIHEATIIIVIAGMEGALPSVIGGLAPCPVIAVPTSVGYGASFQGIAALLG
                     MLNSCAPGISVVNIDNGFGAVYCAYRIIKNLPDTQ"
     gene            8307..9485
                     /gene="larC"
                     /locus_tag="PLJ75_00050"
     CDS             8307..9485
                     /gene="larC"
                     /locus_tag="PLJ75_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HPD03642.1"
                     /translation="MKTLYCDIQLGASGDMLIGALLDACLPVDVLQQQLASLPFTGFA
                     IAPVKQKRYGITGTQAGITAVEDTHHRDFTAIKKIIATTSCSDTVKNNATAIFETLAT
                     AESEVHGIAKDEVHFHEVGAVDSIIDILAFCIAIEYLNVDAIYYNNFAFGSGTITTQH
                     GVIPLPAPATVRLTHNKQVTFTLNNGELTTPTAAAILVTLGTQQPSFSGCIAATGIGF
                     GTRDYGFPGYTRIILLNEIHEEESSYKHELLNVIECNIDDMNPELVPFVADALLEAGA
                     LDVYYHQVIMKKGRPGVVLWALCAIEKTDIVQKIIFSQTTTLGVRAYSVNRSSLSRQV
                     QTVTVYGEKITIKLTSRYHELSPKPEFEDCKRLALKTGKTVRTIMEEALKEYYKHQTL
                     "
     gene            complement(9480..9944)
                     /locus_tag="PLJ75_00055"
     CDS             complement(9480..9944)
                     /locus_tag="PLJ75_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD03643.1"
                     /translation="MKTQTKESVAPSMQIVCFNVGKEEYGIEILKVQEILKLPEVTPL
                     PKSAEYILGVIDLRGKVIPIIDLGVRFGISEKSAQNKRAIVVDIKGKRIGLAIDQVSH
                     VVKLEQKDIEPPPPIVKGISGRFIIGIGKVENKFVIILDIDRIFSADELTNL"
     gene            complement(10203..10439)
                     /locus_tag="PLJ75_00060"
     CDS             complement(10203..10439)
                     /locus_tag="PLJ75_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03644.1"
                     /translation="MDTGVILTIVALLSFIVLFYIFMKQLMKMVVLLLTMFIIFAGYL
                     YVKNGSLPKSLQGYLDEGKIGIEQLQHTYKKLTE"
     gene            complement(10450..10662)
                     /locus_tag="PLJ75_00065"
     CDS             complement(10450..10662)
                     /locus_tag="PLJ75_00065"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPD03645.1"
                     /translation="MRPKIIIILIILLLFLIIVVQNVQPVIFSLFHWEVTLPLIVMLF
                     VTFVCGIIIGMAISSISWHKKKAKNN"
     gene            11051..13705
                     /locus_tag="PLJ75_00070"
     CDS             11051..13705
                     /locus_tag="PLJ75_00070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD03646.1"
                     /translation="MKIKNTFDEHINSLINSFVNEQKVRFPQDEILTIDLHCHDYNSN
                     VPDELLGRILGIPETWLSTDELIATLKNHGCDTFTVTNHNNARSCFELLDKGFDIVVG
                     AEFSCSVPDYGVGIHVLTYGFTSEQEIKLNKYRNDIYKFLDYTSQNDIPTIWAHPLYH
                     YKSRGIPPMDFFDKMALLFQRFEVINGQRDSWQNMLVKQWIESLTPEKIEQLSKRFGI
                     KPDRYCTQPYKKYFSGGSDSHMGIFTGLTGTYLHIPHLMNRLHHEKRSSLALEAIKSG
                     TMAPFGTHNDSEKMAISFIDYFCQIGMKMKDPGLAHILLHKGETIDKLAALTIVNSFQ
                     ELKRHKTTLHFLELFHQSLTGKTFSKSKRFYVNKDYKKVFDIAYTLSRYTKTRKKEDI
                     IFYNDSIYAIYNELAQLLMKRAHKYIHKFLNDTGSATNEFESYFQNIEIPTHFRKYIA
                     PSKGADSNGIPELNLPKIFDELSFPFLGSAVIASAFFTSARVMYKARPLLYEFAQIHN
                     TLKHPERTLWLTDTFNDPNGVANVLQLMHKEIKRNDLPIDIITVSDTLQSDEHLIIIK
                     PLKEYTIPFYQQQPLRIPDILQIHKIFKEGEYNKIIVSTEGPMGLAALWLKYAYSVPA
                     YFYLHTDWMMFAEKTLKLKNDNLDNFRRILRTFYKAFDGIFVLNNEHKKWLTGKDMGF
                     NSSKVFLTSHWVEEIFKPHKTNKEEVFGVSSTTPVILFSGRLSEEKGVMDIPEIMNLV
                     KQKHPETKIAFAGIGPKETQLKTLLPDAIFLGWVDHTLLPEVYSAADILVLPSRFDTF
                     GCVVLEALSCGLPVIAYNNKGPKDIINNGLNGYLVKNETEMAQKIIELISNPLLLNLF
                     RNQSLLRAYEFKPDTIMKQFVNDLNSAA"
     gene            13683..15314
                     /locus_tag="PLJ75_00075"
     CDS             13683..15314
                     /locus_tag="PLJ75_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPD03647.1"
                     /translation="MTSIQQHNKHPLHYVWWKHGVIYQIYPRSFYDANDDGIGDLPGI
                     IKKFDYLIDLGIDAIWLSPINTSPMYDCGYDVSDYYSIDPIFGTLKDFKELLAIAHSN
                     NIHIIMDMVLNHTSQLHPWFVESSSSRYNMKNDWYIWHDGFNGKPPNNWKSAYLSSAW
                     EKNEMRGQYYLHSFLKEQPDLNWHNQQVKHAMFDMMQYWLDLGVDGFRFDVINYCYKD
                     KNLRNNPFSFNPFNDQIEKYNKNRLESYILVQEIRKLIDKYDNRMTVGEVFSYPPGNP
                     ALSASYLCKGKGLHLAFDFSLLYQPFDSHKIYRCIKNWYNHIPEHCWPCNVLSNHDQQ
                     RYADKFGQEDNDSIKRLMALLLLTLWGTPFIYYGEEIGMKNTSIPLRKIVDPAGKRFW
                     PMYKGRDCARTPMQWSDEANAGFSHTMCWLPIDSNYRTINVKHQLKDRYSLLNYYKDL
                     IRLRKKHHALTHGSWEPLIKGKNGVIAYIRKAASETLCILLNFNDHATHIHHHYEAQW
                     KVLFSTHRSRFEHFADLRINCYPFEATILQKIGDL"
     gene            15321..16673
                     /locus_tag="PLJ75_00080"
     CDS             15321..16673
                     /locus_tag="PLJ75_00080"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD03648.1"
                     /translation="MNKIVNLFQKKSLSDNTSLALPVSITHIPIWIIIIGIVLALLIS
                     ISGFMNIYFLNSSHQEIEKINTLTSLALSTQIEFEKQFSMLHYILLEKDPLQYQTYFH
                     KFSYQYTIVQNQLFNLKLLLKDFPNIHNKMNDIQKYHKDLTELLIDELFQYKDKSATV
                     DDVYSIIKGKDIIAIESLNTIISEINNINYEIAMKTKKKYFILSIVSISLISIVTLSL
                     VIAILYITKLEKKFIFNIGKQLSSYLPIQLVQLILRQEKQEVVPIQKKFITVCFTDIE
                     GFTKLTDAIEPEIVAEILNDYLSEMTVVAHKYNGTVDKFLGDGIMILFGAPNSMPASI
                     QIENAMTMAVEMQTIFKMLNDKWKIKINNNILHLRIGIHCGMTTVGSFGPPERLSFTA
                     IGKIVNIAFRLQQLCNSDGILISEDVKNIMPQIPVNEGIALNVKGIDKPLEVYSIQSN
                     "
     gene            complement(16689..17885)
                     /locus_tag="PLJ75_00085"
     CDS             complement(16689..17885)
                     /locus_tag="PLJ75_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD03649.1"
                     /translation="MNNNSQVVSCQPIVHKIEELSCTQFTIGMLISSNYYVFTDTKVG
                     ILADELSRNDKIYAVAVVDKNKKSYGVIIRKDLFDILGTRFGRELNYNKTADEVMQKV
                     KQFNYNMHIFECAKAIEKDLLTGVQDHYLLEDENDCFKGIFSTRDMLIFLSKLNQQDI
                     EQAKRIIERILKKDYYVHTKRLTIGIKNVMAQGVGGDFYIVIPFKKSNQHIIVVCDVS
                     GKGIAASMITTLLNGMMSIYDFETHDLETFLIHLNNYIFTNFEGEKFVTSAFMHVDED
                     KELLTLYDFGHSLTYLYRNDTIFKLSLKEINFPMGIQKINTLNFGKTKLNSGDTILTI
                     TDGCIEQRNKECAAYGVQGITQFMKENYNGDVQKFLDLMVFDINNFRDTYPQQDDMTI
                     ACIKYE"
     gene            18435..18929
                     /locus_tag="PLJ75_00090"
     CDS             18435..18929
                     /locus_tag="PLJ75_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03650.1"
                     /translation="MKKSITKTMLIINFFYISILLLYTQTFADICVAPYQSPSGFSVD
                     LPAWDRSEMMKQDALEFICAHSQYIYIKIIKLKNPPDDILHYMSWKIWDTDPNAHFVI
                     KDKLEDGGIIISTYRNKTYSLHRVRMIQQKEYTYIIECSAPEKSFYNYEKYFNRVFKS
                     FTLQ"
     gene            complement(19322..23737)
                     /locus_tag="PLJ75_00095"
     CDS             complement(19322..23737)
                     /locus_tag="PLJ75_00095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD03651.1"
                     /translation="MSDSHKKQKTSKHDGTSNRKKKQISSTVSGRDLQQLDFYNIIAQ
                     KSADIFTIFDMDFNIIFVSPSIEKILGFTPQEHKKRKLDQILTPDSLQSAFNVYAEEM
                     EKEKAGNVDPDRNRIMELEHYCKDGSTRWLEVNLSMIRDAQLKPLGILAVSRDVSELK
                     DITNQLKESEKQYRILVETAKEGIWRIDSNNVTTYVNAAMADMLGYAQKEMIGRPIFD
                     FMFEDDIPVLKERIETRRVIGIADVYEDRRKRKDGSELWCIVSGQPIMDEKGKYIGSF
                     AMYTDITDRKKAEEKIAEYSTMMENVIEATQTGTWQWNLQTGALIINEMWAHLLGYTL
                     EELSPISIKTWEMLTHPDDVKKAYEDLVNHYTGEKPYYECEIRMKHKQGHWVWILTKG
                     EVIEWTPDRQPLMIYGTHTDITHFKETEQALKESRQLLNNVINTIPVRVFWKDRNLRF
                     LGCNKHFAVDAGFEKTEDIIGKTDYDMGWRSEAELYRMDDKEVIETGIPKIGYEESQT
                     APDGSNLWIRTSKIPLRLGDEIIGVLGTYEDITAKKLADEKLKESEERYRSITETTHD
                     IIVLHDMNGIIQYVNQTGINIGGYGYDEIVGKNILKFIPKKYHQSIVTLHQERINGYM
                     GLHQYETEIINKLKKKIPVEVASSPIVKNGKTVAILIVAHDLTQRKMAEELLVKNKER
                     LESIIAVLQKEAKTEEELFYHVLNEAIKLTDSTIGYAMEYNEEEKRCFIKAWSDVVMN
                     QCAIQDKPVIYNVEGAGIWVETIKQARPVVINNYNDYHPAKRGYPEGHVDIQRFLGIP
                     VFKDNRIVATVGVANKETDYTEIDILQITMLMDSAWQKIEKIKSEQALAESEEKFRSL
                     ANSTPTAILLYQDNKWIYANPAAVNIAGYSAEEFLNMNFWDIVHPDDKQIVRERGTKR
                     QTNVDTVDSYEFRIIAKDGRIKWVYLSGVSVIYKGRPAGMISILDITDRKMAEESLIE
                     EKEKLRITLQSIGDGVIATDTNGRIAVINETAQQLTGYSQEEAQGKPLSEIFVIVHEL
                     SSKPLDNPVEKVLQTQQIYELSNHTMLIAKDGTRRIIADSAAPIKDAMGRILGVVLVF
                     RDMSEKIRLIEAVQRTQRLESLGQLAAGLAHDFNNILEGVMGYIGLASTYIKDNNISE
                     LLGEALKSVQRAKGLTGQLLTFAKGGTPIKKTQSVIPLLKDTVKFALSGSNVKAEFNC
                     DEDLWNAEFDYNQIAQVIENIVINAVHAMPMGGTITVGAHNITFALNEHPLLPAGKYI
                     KIAIADKGIGIPKDILSKIFDPFFTTKSKGHGLGLATGYSIIARHNGTIEVDSIPGQG
                     TTFYIYLPATQQKVEMEEKNKTYEKKLTGGKILVLDDDAMMQKLMISFLEHLGFEAMA
                     VEDGQSAVNLFLHEKEKGTPFNALIFDLTVKGGMGGTEAIQLIRQHDTGVPAFVMSGY
                     HDDQVLANPEEYGFNNGLSKPFTIEELQHLLAKYF"
     gene            24059..24595
                     /locus_tag="PLJ75_00100"
     CDS             24059..24595
                     /locus_tag="PLJ75_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03652.1"
                     /translation="MATAPSSKLPPQLKGEIINLIEERLKELYVTCSDFRALQRVMKE
                     LAEAEKRTEQRIDSLTQRMEELAEAQKRTEQHMDVLTQALTQTQLELKQLVEEHKVTR
                     KLLGDLTNTVGYGLEDTIMKYIHDYALKEYGIAITTVDRRYVTYPDGRYDEINIYAKG
                     TKNGTNVYLIGESKAQLG"
     gene            complement(24789..26468)
                     /locus_tag="PLJ75_00105"
     CDS             complement(24789..26468)
                     /locus_tag="PLJ75_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD03653.1"
                     /translation="MRIIYCTDVHGAFERVRELLYETIADVYIISGDLVDIPFYNMDS
                     AIRYYELQLYFNTMRKKMGRDDITVEDFVDELMDMPHITEEVAELGAKYQQYTIRARR
                     VMQQKYKVLANIFSFKPQVHIFALPGNYDMDLKYTSLHDRDLHLHWYQVDEAVIAGYG
                     GADTFTAGIPQKYVVPYKAGIGIDDRKNEMYNFFKAVRPTIIATHQPAHGVLDWLAPV
                     GPTGSPALRTYCDNNPVILCLTGHIHGSWGMQEVENTLYLNPSPLGDITTVHHDVLEG
                     GFFYQIELQDYTVAHVLYRKIYNERVYDLAEYAKKNGKWVETVIDDIRFSAKKKLLNV
                     DMNVQQYSHIPEIELFKEIKNFFRMFQTEESELRMEKLEEIVKRVEPAIEDIAMDVLG
                     SVNVGLSQPSSDIDMVLYLRCDFECPDHLLTCDCCKNAALLIGNALQEEYKFEVLDCI
                     NLNEVEKDIKDKNYESETLQRFVAYRSICRPINYRVIAPLEDMLNADIEFRKEVEGSI
                     RSYFRIFVTTSQHVRSFKKYESRLKSVGIRLPDSIRRKILQYLQSDEDKEI"
     gene            complement(26468..28066)
                     /gene="lnt"
                     /locus_tag="PLJ75_00110"
     CDS             complement(26468..28066)
                     /gene="lnt"
                     /locus_tag="PLJ75_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPD03654.1"
                     /translation="MNIVKRLAIFLYDNYYLLSVLLMYCSFTTSLNVHLPLLSVFAWV
                     SLVPLFCFVGNKPLKQVYILSFVTGILGFGAVYYWIGDFGQALPFGKPIIVALVIPCI
                     SVFFATKIFVAEYLSRKSNVPKMLIYPSVWIAIDWVQTVGTMAFPWNFWGYTQYPFTN
                     FIQIASIVGIYGITFIIIAANSSISEYITARIMNNNYRQPLWACAIICVIVIFATAYG
                     WVRLHNHPPSKNSTLRVAMIQSCFSPWENWIENRYRYLHILKQLTQQAMQHNPNLIVW
                     SESATLENISYGYYNGNLNRFGEEVLSLAAGYRVALLTGEIGVVEDFVNRRYYPQNSM
                     VLINSNGEVVDTYAKMYLVPFGEYFPYGELFPFVNTIIDAFGGSNFMPGEKMVVLSVN
                     GLKCAPLICYENIFHTLCRKYAKAGIDFYVNITNLLWTNNPVGPMQHFYFSTFRAIEN
                     GVWFVSAGNSGFTALIDPYGRITASIPLMEKTYCIGDIDTSTNTVTVYRRAGDSLLYT
                     AFLFLGILSVMIVFRTYTEKRMVS"
     gene            complement(28063..30057)
                     /gene="ligA"
                     /locus_tag="PLJ75_00115"
     CDS             complement(28063..30057)
                     /gene="ligA"
                     /locus_tag="PLJ75_00115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPD03655.1"
                     /translation="MEDIKKRYDELIKLINKYNYYYYTLDKPLVDDATYDELMNELLT
                     IEKNYPEITRDDSPSKKVGATIQTSFEQVRHDPPMLSLANAMDEGDINDFYERCCKLL
                     GTSTIEYCAELKYDGLAVELVYSNGIYVQGSTRGDGEVGEDVTENIATIKRVPTKLPG
                     DAPDYLSVRGEVIMRHGEFERLNEIRRQNGEQEFANPRNAAAGSLRQLDPNITAQREL
                     DIVLYGIGKMEPASMINTQSQLYDYFKKVGLPAPHYYEVGLLQGIKQFYKYWMENRYT
                     LDFDIDGVVVKVNDITVREKMGSTSKAPRWAIAWKFPAREAITVLKSVDYQVGRTGLI
                     TPVANLEPINIGGVLVKRATLHNFQEVKRLDIKIGDTVKVIRAGDVIPKVVEVIKDKR
                     KDVRPIAVPETCPACNSTLQHEDIYIRCVNASCPAKEYETLCFFVSKDGMDIEYVGPE
                     LLKRLYDQKKIKTIADIYALKREDLLQVERMGEKIADKIIDSINKRREVTLSHFLRAL
                     GIRNVGDHMAKVIAKHAGSLQKLMVMSQEELMTIPEVGPGVAQSVYEFFHNEGSLTLI
                     RQMFANGVTVKDEKVQEASDNPFKGKTVVFTGTMQNMTREEAEGLIESMGGRASGSVS
                     SKTDFVVAGEEAGSKLDKAKKLQIRILSEDEFMTMIGKHQ"
     gene            30502..31485
                     /locus_tag="PLJ75_00120"
     CDS             30502..31485
                     /locus_tag="PLJ75_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03656.1"
                     /translation="MQLKKEIKKITIKDPFQQFKETERVNEMRYCPLTGRVSRVLGLS
                     LKEFASNDNTHIIEESKKTCPFCPQCIDALTPKFTPPLQEQYSWGNARCFPNAFPYDE
                     NNAVVTITDQHYVALDKFTHDMLSDAFHCATNFLSDIDPVQYPYQSINCNYMPAAGSS
                     LVHPHFQIIASSTPSSYYTDILSNITIKPNLFHDYINTEKEQNERIIGLEGDSSWLVA
                     FAPMGQFDVIGILHNHIPLKDFYRYCNTFITYILKILQYMASKKIDNFNMALYGVTNN
                     ADFLPHIRICPRTTFPPYATSEINYLQMLHNDSFTLLKPEIIAAEINAYTP"
     gene            31772..32731
                     /locus_tag="PLJ75_00125"
     CDS             31772..32731
                     /locus_tag="PLJ75_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPD03657.1"
                     /translation="MLQSILYNYDQRIHLEELLTFSMALLLNILIIIFSHFTLAPVTF
                     TINIAIMLLIIATVFAQRFATSKLFSIYRDWYVLPLLVAIFMEHNMLVPLINPNDIDT
                     YLIKIDYSLFFGNHPTLLLERVMHPFVTELLQIVYASFYFLPFTLCLLLYLKERKVEF
                     HIVASSIMFGFYLSYIGYYIFPAIGPRFTLAQLQTKPLEGLFLFNFVHHGLAYLEGVT
                     RDCFPSGHTLVSILTVLLAMRYHKRFSIIATVWTVLLIISTVYLRYHYVIDVIAGFFV
                     GLIAFRYGQMLARLYIFGNTETKTPLLESIRNLWYWLLDIRRR"
     gene            complement(32739..33467)
                     /locus_tag="PLJ75_00130"
     CDS             complement(32739..33467)
                     /locus_tag="PLJ75_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03658.1"
                     /translation="MRRRISSKPIILPEIKKTYWKRRPSEIRGYQRVFYCNMENGDYI
                     RVDYSFKDKRVRIYLEDSEEGGNPYYAVIDKGRITAERNATSGRPVPLQEKLEKRSEI
                     LSTLPNKEVLRLINKHYGIGETPEEVAARKKKRQEELARTKKRYFKPEEVPSFVKKET
                     ALPTKRYVRIRDIVDIAVGGTIVGVVYYVSMDFVKMGIASAVFGIIMAFVDIFVRGKE
                     TLLLKTLLFLLTGIMLYIYGYFVL"
     gene            complement(33813..34697)
                     /locus_tag="PLJ75_00135"
     CDS             complement(33813..34697)
                     /locus_tag="PLJ75_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03659.1"
                     /translation="MSKYKKLVIAAFIIAAAVYIFVKAVVFVGDDVVAIVTDKEDQII
                     GLLKKGANVVPQALFHPVRVNYIPLHGSCKADIIVPIPPLEQIKSKNYSITIPVSIQY
                     TFDADKLQLDLKSMHSNAEYVKEIITIALVDAVNTQFRKYLVPVYKYFPLQANFQNEF
                     TVVINTVKEVCVKRGLVIDDVKVGNVYLPDYAIYAEGKRFLLEVLQQEKDNALQLARL
                     QQKLNEDGLASKQRIEHLEKISELIQKNKDILRYIYIDKLADNVKVIVTSDKEAGFDI
                     FNTEDSGSVKKDVDNFKK"
     gene            complement(34701..34931)
                     /gene="hfq"
                     /locus_tag="PLJ75_00140"
     CDS             complement(34701..34931)
                     /gene="hfq"
                     /locus_tag="PLJ75_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HPD03660.1"
                     /translation="MGKQTKNLQDTFLNAARKDKVEITIYLMNGVPIKGKVISFDSFT
                     ILVEVDKKQNLIYKHAVSTIMPSKPVQYKDEE"
     gene            complement(35045..35959)
                     /gene="miaA"
                     /locus_tag="PLJ75_00145"
     CDS             complement(35045..35959)
                     /gene="miaA"
                     /locus_tag="PLJ75_00145"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPD03661.1"
                     /translation="MKKVNKKKAVILVGPTASGKSDISLSIANNLFEIVSVDSVQVYK
                     YMDVGTGKVSPYHRSLITHYCIDIVNPDYWFTAGDFCREASGAVETILQHGKIPLFVG
                     GTGLYIHTFFYGLAKLPRIDSKIKEQLHAELQLKGIQALYKELSHHDKAWADKIHPND
                     TQRILRGLEVFRATGLPLSQFLQSDHMKSDLEILLIAVDIERDILYKRIECRVDTMIN
                     NGLVEEVEKLRAMGYTQDLNSQKAIGYIQLHRVLNNEISLSDAIEDIKLQTKHYAKRQ
                     ITWFKRYKPLWMPANDGKGIKKIINEWI"
     gene            complement(35988..36191)
                     /locus_tag="PLJ75_00150"
     CDS             complement(35988..36191)
                     /locus_tag="PLJ75_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03662.1"
                     /translation="MIIPLDKIIALEDNKYIFSCAAMKVVDKLGNIEGYPETDKNWKV
                     VPHILHLMLNGIIKYEFKEQLSD"
     gene            complement(36172..36789)
                     /gene="gmk"
                     /locus_tag="PLJ75_00155"
     CDS             complement(36172..36789)
                     /gene="gmk"
                     /locus_tag="PLJ75_00155"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPD03663.1"
                     /translation="MIAIIISAPSGAGKTTLIEKLIIQNPIYEFAISTTTRPIRSNEK
                     EGKDYYYVSYDEFMKMVDNNEFVEWAKVHDNYYGTTKKEIDRINANSKIPIFDVDVQG
                     ARALRNKLQKVVSIFIIPPSIKELEKRLRHRSTETQQQIKIRLTNAVSELREYINYDY
                     IVVNDDLQKALEDLGAILKAIILRREFKEKAVKNLLEGVHDYTIR"
     gene            complement(36789..37043)
                     /locus_tag="PLJ75_00160"
     CDS             complement(36789..37043)
                     /locus_tag="PLJ75_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HPD03664.1"
                     /translation="MKSTTLINIGFGNAVVAERVIAVITPVSASGKRLREEAKENSML
                     IDATHGRKTRAIIIMDSNHVILSAMQPETIANRLNQSEEG"
     gene            complement(37057..37923)
                     /locus_tag="PLJ75_00165"
     CDS             complement(37057..37923)
                     /locus_tag="PLJ75_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPD03665.1"
                     /translation="MESMTGYAFIEQSTDQFTFSIEVKTLNSKYLEVYTNIPKILKYE
                     DDTINAILKKSFERGKVEFNIDIYDWVESRPVHINKQILLKYYHEVQKALSSLKIAAS
                     FSVDALLQLDGVVHKERSILSKKSKDDIYNALQKSINATIKMRKKEGKSVKKDLESSL
                     KIIEHACIDIRKQAHAVAEYQFSRLKKSIESLLHSKIDENRLYTEIAILADKQDINEE
                     LTRMYDHIVKFKEIMAGEGQLGRQLDFLAQEMFREVNTIGSKSNNTVIAHKVVEVKNH
                     IDKIREQCRNIV"
     gene            complement(37936..38415)
                     /locus_tag="PLJ75_00170"
     CDS             complement(37936..38415)
                     /locus_tag="PLJ75_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPD03666.1"
                     /translation="MEFQLITVSIADYKVSHSPDILRTILGSCVGICLYDLENKVGGL
                     AHIMLPENNDKSTNPKKYADSAIALMIDEMVKKGANPELIVAKIVGGASMFKMPENSF
                     IGSIGINNVLKVKQVLDKYHINIIAEDVLGDYGRTVDFFLESGKLKIKSLGREDKEM"
     gene            complement(38415..39266)
                     /locus_tag="PLJ75_00175"
     CDS             complement(38415..39266)
                     /locus_tag="PLJ75_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPD03667.1"
                     /translation="MIDFTNIKRLTEDEFYKFAKLIYDESGIFLKESKITLLSNRLRK
                     RLRALNFSEFSDYFNYINALKDKSKEIEELLDVVSTNETYFFRNERQFDALKDHCIPK
                     IVKMKNEKKLKIWSAGCSTGEEPYTIAICVLESRLLLSDWNVSILATDIAPSVLEFAR
                     IGKYTGRRIEKVPPEILKKYFTQMPDNPEVYEVKDELKKLVAFSYLNLFKNSYPENVD
                     IIFCRNVMIYFDRSRQKELIANFYNILNPWGYLFIGHSETLHALSDDFQYVKIMDSPV
                     YVPKEKV"
     gene            complement(39298..40164)
                     /locus_tag="PLJ75_00180"
     CDS             complement(39298..40164)
                     /locus_tag="PLJ75_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03668.1"
                     /translation="MQLIILIMVNILIASVFYLIISLKLERSATEFRSKRLRKEMEEL
                     INEFNNTAERNLSLLEYKINILRELLKRGDALNGIDITIDDENGQPVPAEKSKTRSYN
                     RKNHLNENNKDSRNKHDTIVAEIINPHNVVNKMQNDFDNGNSIHKQNTIESAIIKENN
                     EIPTVKEQLLELSRVIVARIKENIKYKYKKNVAIKDLPDVHYNEMQDMSTTSFQSDSY
                     KPEESMIKTPEEDTLNVDETVLQQMYVNADDKYLFIAKLYSQGYTLQILSKISGIPIS
                     EISLILNLSTRQ"
     gene            complement(40184..40495)
                     /locus_tag="PLJ75_00185"
     CDS             complement(40184..40495)
                     /locus_tag="PLJ75_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03669.1"
                     /translation="MVIKPIDLQTNIAQMIEVARGEHSRNVAAVEQQHILSKESQDKA
                     NIQKEKVEENKKAERTIILNEEKQFKKGKQQKEGSKNDKKGNQEIFEEEAIGKIIDVK
                     K"
     gene            complement(40523..42493)
                     /locus_tag="PLJ75_00190"
     CDS             complement(40523..42493)
                     /locus_tag="PLJ75_00190"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HPD03670.1"
                     /translation="MKNLKELLKTLKDDDIAENEVEVYADSVKQALELASRDLGVDIS
                     ELDYQILEKGTSGLLGIGRQPYKVLIRPIKVEEEEDLTAIEQKLHAVPEADLKVQDVA
                     RNVDGSAKIRITKTGIWLTVTPPKGGGRRVDLSDVSNRIYSLRIQNADMAKIEKEIKH
                     PSGKPVKIGEWIPNPEYDGSMTVEITDDEMKAYIHFVPPRFCGRHMDVDDVIEALKRS
                     GIVTGIKEQRIAEYLDRMDYSQPLLAAEGTPPRHGKDAYIDYKVRIDKSGIQLEEDEK
                     GRVDFKDLDLLENVVVGQVLAVKVPAEEGVPGRTITNRIIPARSGKDIQIKYGKGTIL
                     SQDGTELTAEINGQVVYQNGKLSVEPVYVVKGDVSTETGNIVFLGSVIVSGSVQDNFV
                     VKAAGNIEVKGTVQKANLEAEGDVIIRQGIMGREGAKIESTGGSVIAKFIQTANVLAE
                     KDVIVAEGIVHSNVDAGNRIICNGRRARIVGGLIRAGEEVNSKYIGSDSFTKTTVRVG
                     MNPKVLQQLNELEENKRSINEEFDRVRKDVTTLTVQKNNAGGHLPKDREELLGKLVAQ
                     KQKMEQRLNEINLSLEELKAYLAMLEQKGKVCVEKTIFPGVEIYIKDKRFNVKDPYSY
                     VKFSIEGSDIRLSEYEPPDLDEAQKLIARRRR"
     gene            complement(42546..43346)
                     /gene="whiG"
                     /locus_tag="PLJ75_00195"
     CDS             complement(42546..43346)
                     /gene="whiG"
                     /locus_tag="PLJ75_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HPD03671.1"
                     /translation="MANKKFQGFDATDEDELWKRYAKTQNQDIRDYFVIKYAPLVKYV
                     AGKIAMGMPSSIEFDDLVSYGVFGLIDAISKFDPDRGIKFKTYAMTRIRGAIFDELRS
                     IDWIPRSIRQKAKQIEQAIAELENKLGRTVEDEEIAKELGVTIEEFQSLLNKLSGTAM
                     LSLNDIWYVGDENDELSIMETLEAPDTLNPDVMIEKEEIRDYIVEAIKKLPDKEKKVI
                     VLYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLGRIKSNIVG"
     gene            complement(43365..43919)
                     /locus_tag="PLJ75_00200"
     CDS             complement(43365..43919)
                     /locus_tag="PLJ75_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03672.1"
                     /translation="MLNIKKIAEISPEAKVSLIFGFLAFIVSLFFGLAVGNRIVILLT
                     RVLFFTIVYATIGYASMLVIKHFVPEVYEILNKVLNIGQSLDMATESSDVTMQSEYKN
                     SDDSDKGEGIVSSDSGNESIDVGATYNEAFTPLEAKDFTTFTTEESKPKKMGKHIVLD
                     DKKIHYEPKIMAEAIRTMIKRDKD"
     gene            complement(44039..44917)
                     /locus_tag="PLJ75_00205"
     CDS             complement(44039..44917)
                     /locus_tag="PLJ75_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HPD03673.1"
                     /translation="MDQAANLRRLVVENNTARRTKTIAITSGKGGVGKTSIAVSLAIA
                     LAKRNYSITLLDADLGLANVNVVLGIIPKYNLYHVIKGKKKLEEIIIDVPEGIKIIAG
                     ASGFHQLANLDVKQREYFIKSLAELNADDYMIIDTGAGISQNVLSFLVASDEVIVITT
                     PEPTSITDAYGTIKAIAANDPDKTVKLLVNRAQTVTEAKKVAQRVINIAGQFLNIKVD
                     NLGFIFDDLYVAKSIRNQKPFIVSYPKSKASTCVEIIADRIGNIESDIDKGTGMLSFF
                     RRFFGHYEMDNDDGVV"
     gene            complement(44929..46242)
                     /gene="flhF"
                     /locus_tag="PLJ75_00210"
     CDS             complement(44929..46242)
                     /gene="flhF"
                     /locus_tag="PLJ75_00210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HPD03674.1"
                     /translation="MRYVKIKARTYNEAMMKLKMEHGDDAIPISHKNIKEGGIFNTGL
                     FARDFVELTAAIPDVKPSKLKPRKNIDLTVGGNENINDLLKQVIDKPESQPQQKPNTG
                     LEQLIVQGMEKPNIKTTESPHPVKSPVDEVMVERTDQPYIKLEKEFQELKMLLKNMVA
                     SGQKKPIDDIVIDSNDIYFKPFKEILRKNDIDDDECNRLLEDVKRNVSSEDMKDRYKI
                     EKTLRELIKSKIIVSGPIDKGKRKKVLMFVGPTGVGKTTTMAKLGAILSLRQGLKVSF
                     VTIDNYRIAATEQLKKYAEIMRIPIHVVTDSHEFKEIVENEKAEIIMVDTSGRSHRND
                     LKIAEIKNFADACNCEIHKILCVSANTKRSDLNEVFKAYDILHIDSIIVTKVDETSYI
                     GNVISIADKYNKPISYFTNGQEVPNDIAVADPEIMVNMILESTMR"
     gene            complement(46276..48357)
                     /gene="flhA"
                     /locus_tag="PLJ75_00215"
     CDS             complement(46276..48357)
                     /gene="flhA"
                     /locus_tag="PLJ75_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HPD03675.1"
                     /translation="MAERIYDFSWMQKSDMLLGIGVIAVVTMLVIPLPTFLLDLLLAI
                     SLMLGILILLVVMYVPRSFDFSVFPALLLITTVYRLALNVSSTRLILLQGAAFDGKII
                     RTFGDFVVGGNYVIGFIVFIILTAVQFIVITKGATRTAEVAARFTLDAMPAKLMSIDS
                     DLSNGIINEEEALHRRREVRKEADFYGAMDGASKFVQGDVKVGILITIINIIGGFIIG
                     MVMRGESFNVAIHTYTLLSIGDGLVAQIPSLMVTTATGVIVTRAVSEESLGKDLSTQL
                     GSQPRALMITAAALGLSAIIPGFPKITLILLAAGLGALGYLMKQTEEEKMQKTKIEQK
                     EAALKTHKPESVIPLIQVDALEVEIGYSLIPLADPDQGGTLLDRITNIRRRSALEMGL
                     IVPPIRIRDNMELPPQDYSILIKGVEVGHGSLQVGKLMAMDPGTVEEKIEGVEFTEPV
                     FNLRAIWIDPDNRDVAERRGYTVVDCPTIIATHLTEIIRRHADEILGRQEVQQLIDTI
                     KNDYPAVVNELIGEKKLSIGEIQKVLQNLLRERVSIRNMVTILETLASYSDYTKDIGL
                     LTEYVRVALARQLCREYADKNNTLSVITVDPEIETIIRSSIHEDPVEGRIISLDPDTH
                     REVLDSLFDSYSRARSAGYAPVFLVSPHIRSVLFTLLEREVPDPVVLSYNEIVPEIRV
                     NVIGTALLRAA"
     gene            complement(48372..49532)
                     /gene="flhB"
                     /locus_tag="PLJ75_00220"
     CDS             complement(48372..49532)
                     /gene="flhB"
                     /locus_tag="PLJ75_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HPD03676.1"
                     /translation="MKWYAITDKDAEIVIFESRAIVFDMQLFAAEDEGRTEEPTEKKL
                     REAREKGQVAKTEELPQALVIIFAMITIVVFCGFIFETIIRMMHYYLTNFSHFNLTER
                     SLFHEAIASGVESFKILIPIFVATVIAALIGNIAQVGFQISTHPLKFDLSKIKFDPAT
                     MARKIFFSRQIAMNLFKSIFKVVVIGFICYLVIINDFEELIKTPDISVGLAFQNVSII
                     ALKIIIWAAVCILVLSIPDYVFQKREYIESLKMTKQELKEEWKETVGDPHVRARLQEM
                     QRELIMRNMIREVPKADVVVTNPTHFAIAMRYEPEAMYAPMVIAKGVDTMALTIRQIA
                     LDNSILIIENRPLAQELYKRVDVGDIIPEDLFYAVSLIYAEVYKKRNYKVAM"
     gene            complement(49529..50308)
                     /gene="fliR"
                     /locus_tag="PLJ75_00225"
     CDS             complement(49529..50308)
                     /gene="fliR"
                     /locus_tag="PLJ75_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HPD03677.1"
                     /translation="MEYFVYQFQSFFLVLVRMTAMMMIAPFFSSGVIPLLMRGALSFF
                     VSLVIFPLLSISAGAVTGNMGVYYLMVVQEIIIGLYIGFLVSVVFSAFQLSAQYFAVQ
                     IGFGINEVIDPLAQVSIPLIGQLKNFIGLLVFLTINGHHFMIKAIYNSYELAPAFGLK
                     GDSVQVFLKFLVHSFSGMFVIALKISLPVVAVVFLMSVSMGILAKAAPQMNIMMLGFP
                     FKIAVAFGMMILISPLIVRVMNVSLERTFQFIVKVIRYWPV"
     gene            complement(50308..50580)
                     /gene="fliQ"
                     /locus_tag="PLJ75_00230"
     CDS             complement(50308..50580)
                     /gene="fliQ"
                     /locus_tag="PLJ75_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HPD03678.1"
                     /translation="MTDIEVIKLLREALMVTMIVSAPILGIGMFVGLIISIFQTTTSI
                     QEQTLTFVPKIIAIFAAFILFAAWMLHTLMSYTKELFMMISKLGGG"
     gene            complement(50590..51327)
                     /gene="fliP"
                     /locus_tag="PLJ75_00235"
     CDS             complement(50590..51327)
                     /gene="fliP"
                     /locus_tag="PLJ75_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495865.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HPD03679.1"
                     /translation="MGIGMIVSDAAFAQKAGNVRMPIPKIGFDIEEAKTPKDVSLSLQ
                     ILFLLTILTLAPSIIMMMTSFTRVVIVLDFVRRALSLQQMPPTQVIVGLAIFLTIFIM
                     SPTLTEINDKALQPYLKGQISNEEFYNKGMQPLREFMLKQTRKKDIALFIELAKLERP
                     KTQDDVPTYCLIPAFMISELKRAFEMGVYIFIPFIVIDMIVASALMAMGMIMLPPIMI
                     SLPFKIVLFVLVDGWNLLTYELVRSFM"
     gene            complement(51391..52182)
                     /locus_tag="PLJ75_00240"
     CDS             complement(51391..52182)
                     /locus_tag="PLJ75_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HPD03680.1"
                     /translation="MIVAATIKKRLCVSSMKFLYGVILLCLIFILCPITSNYRPNENF
                     LYAKNTKTAKLDKIESKNTITEKKEEPIGVETKSDDEQKENEASKNDYLYNYERPQIE
                     EESYVWLIFKTIFVIGILIGGFYFFFKFITEKVGIAAVGQEVITILSVVPLGQNKFLQ
                     VVDIAGKVYVLGVSDNNINLITEIKDKDDVDRIRLLSSKSTPVKPGGFQDYLSTQLGK
                     VLDRIKNPKEGISQHYSYNQEHTGNDNVLLTYKQRLKKLNGDDSV"
     gene            complement(52217..53269)
                     /gene="fliN"
                     /locus_tag="PLJ75_00245"
     CDS             complement(52217..53269)
                     /gene="fliN"
                     /locus_tag="PLJ75_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HPD03681.1"
                     /translation="MGDGSLSQDEIDALLQGADDMLSGSARVPAGDFAPVQAALSPVE
                     REAVADFLNSSMSSVMPALSGYLGKNIIIGNALVEVKNADQIRADFPKQYVQVSVNFS
                     GGVNGRNIVLLSMNDAGVISSLMMGDDSGIPPATLSEAHQSTLQEFVSQMMSTIATQL
                     SNKIGKGVNTTAPDLAIVQGDIAVPGTPDVVKITYNLTIQNLINSKMYHLMNIELAGE
                     IARGSYVPQMQQQEYRTEAAPQKSAQVGISPVRFPPLGEGMQSVPTGNINLLLDVPMT
                     LTVELGRTRQLVKDILGLGEGSIIELDKLAGEPVDLLVNGKLIARGEVVVIDENFGVR
                     VTDIVSPAERFAKMQT"
     gene            complement(53357..53893)
                     /locus_tag="PLJ75_00250"
     CDS             complement(53357..53893)
                     /locus_tag="PLJ75_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPD03682.1"
                     /translation="MGDEERVNVEEVEEQEKPEEAKAGRVFETSRIIKILLYVAGVIL
                     SFFIIIGISYLVAKYVQEQRYQRDQDIVLAPPPSPLAHFDIPSFSVTTNDPEPHFAKL
                     TVSLGYEENVELNAELVQRTPQIQHIINILLRDKSYEELNSVEDTVNLSEEIKAHINV
                     LLLKGKIKEVWFREFVVN"
     gene            complement(54226..55002)
                     /locus_tag="PLJ75_00255"
     CDS             complement(54226..55002)
                     /locus_tag="PLJ75_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD03683.1"
                     /translation="MKRKKQQLPPPPPWLTSWGDLNTLLLTFFIMMFDISPIVGQDFN
                     LILSSFKGSLGMMQGGFSLARGRMEEIGLNMLNLPSSEQGRALAKMLKRAVEAFKPEI
                     QAKKVRVREDERGLVITLSSDAYFEPGSAKLKDDIIPVLKKVAEIIKSMPNYARIEGH
                     TDNRPVPPRGLEEGYATNWELSSARAVNVLRYLVEAAGVNPKQMSAVSFGEYRPIDDN
                     NTPEGRAYNRRVEIVILRDKGIVESKTPEIARPLPDEEWR"
     gene            complement(55002..55781)
                     /locus_tag="PLJ75_00260"
     CDS             complement(55002..55781)
                     /locus_tag="PLJ75_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HPD03684.1"
                     /translation="MDIATIIGLIVGFTNIIMGIIFAKGNPLLFFDIPSVFITIGGSF
                     CALLISNPLSRTLGLWKVAKHAFFPPKYSPNELIITLVSFSEKARREGLLALEDDLDE
                     LDDQFLRKGIQLVVDGTDPELVRRIMETELENIQVRHDEGKKIFDDWATYAPAFGMIG
                     TLLGLVLMLVNIEDKSAIGPGMSAALITTFYGAIMAYLVLYPISAKLGYLNNQEMLMK
                     LIMIEGTLSIQSGDNPRIVKDKLVSFLDPKLRAAITSEVGD"
     gene            complement(55875..56066)
                     /locus_tag="PLJ75_00265"
     CDS             complement(55875..56066)
                     /locus_tag="PLJ75_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HPD03685.1"
                     /translation="MIVVHKMNGDEIILNANHIETIESKPDTIITLVNEKKYLVKESK
                     DEVLSLIYDYFHKVLTGKT"
     gene            complement(56063..56971)
                     /locus_tag="PLJ75_00270"
     CDS             complement(56063..56971)
                     /locus_tag="PLJ75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPD03686.1"
                     /translation="MQQNLYQALNQGLILERYTCIQRNIEKNKVLIAKWGGLQKVVSL
                     LSGKHVVVCGAGKSLDDATKVLKKYQHRNDLIIIAADMAYRPLIVNGINPHFTISCEA
                     MPVPYYAFLPTSSCHLLAFSGVNANTTGIWKGKISFYNWMIYDEPFNLLWKMAGEHLG
                     YVATASIVTTQAISLSLGCPIASLSIVGNDLAFNHSYYAGNTVVGERFLMGYNRFITA
                     NTQDYAMIQKKKMYIVKRRESIYYTDHQFLAARQWLEDLFSKVTIPVYDCSVPGCGEN
                     IVKKMTIAKVMNQLYPEKKPKKKRQS"
     gene            complement(56990..59872)
                     /locus_tag="PLJ75_00275"
     CDS             complement(56990..59872)
                     /locus_tag="PLJ75_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03687.1"
                     /translation="MSKRFLLYIAIMMLCLTNRGVFGAGMFHPCYQWYSIKTQHFWIH
                     YHDGLQKQALHLAPIAEAVHETLTREIGLEPFFRTDVVLCDTTDMANGFSMPFPYNRI
                     QLFIVLPHVEDAGLSNNDDWLRLVFTHEYTHILTVDMIGGLPEATRYTLGRACFPNLL
                     MPLWAIEGYAVEQESKEKPWGRLHATYTMMVLRQEVYANNVKDISLASHFPREWPAGM
                     VPYLYGGLFADYVSRNYQTNDFSALFKENADNVLPFLMEKNFNDVYNTGAIVLWKKWQ
                     EELHTAVHNAVDSITKRGLSSYERITSTGYHTSMPRYYGNDIVFATVTGKQKPALMLY
                     STATKQCKKLASVNDPQSISVAGKSIIVSDIEYYQSFNLFRDVFVYDTNYRQKTKGLR
                     VQYIEALSNGYCAIVYSKGRYSLVRLNASFAINDYCIKDTPVQLAYLRVSPDSSQAVF
                     SIVNKGCSNIALYDFKTRGVVLLTDDRYTDIHPAFHPDGKRIVFSSDRDGVFNLYEIN
                     LSNKKISRITNVIGGAFFPDISSDGNTIVFSSYEANGYNIALMPYTTTLSGELSPKPF
                     TVPEEQLTDEHIGNPYNPLHSLIAPWWYPVWYTEELYTDKYDNHAGFLTSGNDTLYKH
                     SYMLEIDYALTQQQMEVKADYIYSGFYPDMFVSYKDDSIGFDDTFPWDPPSNVHYLRR
                     ESEQKITSGFAVPFIKFYNYNQLLLYYTYEKTRIDEYYYTAGTQRYREVLAGGHVALI
                     HDGTYVGSYSISPEDGMIVELYGDMYHTSVASDISYAKVRSGLYYFLRCFANDVLAFM
                     LRGGYAHNAPKYLYPYTIGRYEKGKRHSVPNDEDAYSMRGFTKDEYYGNRLAVAIVEY
                     RIPLVQKDMGYKLVPLLFRDLWLTPFAEYGNIWVGETDIHDFNYSFGSELHLRITAGY
                     HVDIEGFLGVVKGYGDYGETQVYFGVATVFEGALKQHTIIRDAIYN"
     gene            59847..60578
                     /locus_tag="PLJ75_00280"
     CDS             59847..60578
                     /locus_tag="PLJ75_00280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD03688.1"
                     /translation="MYNKKRFDIFSSQYKPDIISIEILFAAIDSFADYVYPLFAGNNK
                     ITIIMFDSYYMKKWQVESRDTVSRQTVFSVEKLLCYHPQKKIKHDFYIVNNPDWVNIV
                     AVDKDGNVILVKQHRLGTDEYTIETVAGLIDPGEDPLQAASRELLEETGYKAETFILL
                     KKLAANPAIMNNYIYFYCALDVHKVASQALDIAEDIEIVTLQPGEVISSMKNGTINHS
                     IIVTALLLFFMSPYCSYPKEDIVIY"
     gene            60588..60998
                     /locus_tag="PLJ75_00285"
     CDS             60588..60998
                     /locus_tag="PLJ75_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03689.1"
                     /translation="MIKSIYIIALLFLQPTSDFSITLSYHPQPFYHQEWFITLARSQS
                     KAYIATNNPQYLRKKRTITINEYATIVQQLNSLGIWHCKDRYIQGNYYMLTITNRNYR
                     QQCKIEYSPSIPGNNNYSQIIRIILNTANNYLNN"
     gene            61097..62134
                     /locus_tag="PLJ75_00290"
     CDS             61097..62134
                     /locus_tag="PLJ75_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03690.1"
                     /translation="MKIKYLILLLPIIMIFSTVTFSQQATEENVIQPDVQSPVSPEVK
                     KEEIVTAALKAVSFNNGVNTFVNSKVKFELIAKDNIATDKILYKLDAGNELEYKEPFT
                     IYDEGKHTISYYSIDKVGNKEDMKTFKVIVDNTPPVVNVAVSAPVIKSGDKLYASKNF
                     TFTIDAKDALSGVQSLEYSFDKTNYQPYLTSFTIPVEGAITLSIKAMDNVANYTEKFV
                     MTAKDENGNPIDLTNGNVAVYVDNKAPLVEIRADKEFLEKDGKKIATVDYNYTVSASD
                     TESGLSSILVRMDGKGEFKAYTGTIKFDTNGEHMIEAKAIDKVGNISSTAVLSVFVDV
                     IAPETLIKSVE"
     gene            62202..62537
                     /locus_tag="PLJ75_00295"
     CDS             62202..62537
                     /locus_tag="PLJ75_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD03691.1"
                     /translation="MFIDTIKYDSILVITPHEDIIRENSEDIELAVKNNYNKSITDII
                     LDCNHVQYINSFSLGTLVKIHQNLQENNCKLHFLNVAPSIKTLFKITHVLDYFSILNS
                     IDEVKEKSK"
     gene            complement(62529..63017)
                     /locus_tag="PLJ75_00300"
     CDS             complement(62529..63017)
                     /locus_tag="PLJ75_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03692.1"
                     /translation="MLNKIIITLLLLLQSDDSKFSSSLCNEAKMYAKNNNYNEAGELF
                     LKAASINPYYFYAHYGYGKVCLYTGDIKNAAKHLSIAVTLDASSANGWFYLGFAYFFS
                     KHYMNAVSCFKKAYTLDSSYIESLYNIGVIYEMMGNSFLSQNYYKKYYFAKNKKDTGI
                     LF"
     gene            complement(63007..63450)
                     /locus_tag="PLJ75_00305"
     CDS             complement(63007..63450)
                     /locus_tag="PLJ75_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03693.1"
                     /translation="MNTTMLSTSMMMFSVIWGLSVVLLWMRPRIEILWKIAASGIYGF
                     YIWFFYKDLLKGFKQFQKDWYMVTIAFIKEFSGLLFANMFFLWPLALIIIFYKADDVG
                     AERLLKFMCIFTLLVWIVFTLYTFYSKQIDTFLFETLRQMIPDAQ"
     gene            complement(63464..64444)
                     /gene="pdxA"
                     /locus_tag="PLJ75_00310"
     CDS             complement(63464..64444)
                     /gene="pdxA"
                     /locus_tag="PLJ75_00310"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPD03694.1"
                     /translation="MQNKVPVIAITIGDPAGIGPEVTLKALSNFSALLFVPLIICRTE
                     VLIKHYHDIMLPWDSIALNDLQRIHTIDKPCYVTVDFDGPVPNPSNGNELTGKESLCY
                     IDAAIDLWKKNIVDAMVTGPVNKSLIEKWVPFIGHTEYIAQRIHEPNPRMLMYSGRYN
                     VLLVTTHVPVYTLSYHINYESILTTVEICNKYLKKILHKQPVLAICGLDPHCGDKGAI
                     GAFDTDVTAKVVKDAVNNGILIEGPFSADTLFMPQRWQTYDCVIAFYHDQGLIPFKMV
                     AFDEGVNITAGLSIVRTSVDHGTAFDIAGKNCAQFNSMKRAIELACYLSK"
     gene            complement(64434..64856)
                     /locus_tag="PLJ75_00315"
     CDS             complement(64434..64856)
                     /locus_tag="PLJ75_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD03695.1"
                     /translation="MALAKSGGDVANNVIGEDSYFSGKFLINGSLRIDGRFEGKYLQA
                     DQLYIGPNGKVKTNINAVSVIIEGLVIGNINATNRVLLMPTAKIYGDIKTPELIIQNG
                     VILEGRCTIANDLKISAKDLIESEYEKNKLIPEEFLAE"
     gene            complement(64929..65702)
                     /locus_tag="PLJ75_00320"
     CDS             complement(64929..65702)
                     /locus_tag="PLJ75_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03696.1"
                     /translation="MEEREEKARQIVRSIRALEGVLKTSPDPKQRERVKNDLKALKGK
                     LSELYPDVDLNELIEAIVTDDLVAPVADKSLLEAFEYLKDIEIEKISNFKDDSEINIA
                     ASILKHFSEKIWGVIADQYTKLDYSISLERDTLYRKLDECQRALKIFQQTVDDIEKAN
                     SSAHISQLNLMRSRQGRLFLIDLFAFFKNVNDFITKILADLEFNGTMVLNADDIITYA
                     KYDVYKTFEGLTVKEALRHLKAFVKEVLIIIKVPDKTKF"
     gene            66037..66948
                     /locus_tag="PLJ75_00325"
     CDS             66037..66948
                     /locus_tag="PLJ75_00325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD03697.1"
                     /translation="MKLTTVYPVMVLCLIWFHNHSYAQYTIPVISELSRNSQSIMQIR
                     KDIRQSLYTIKSNRSVNELPTLSMYKYVVKGSENFWDIIAVTNLDMDTLISINSLSSP
                     SDIKKGISLFIPNMRGIIYQKNENESYESIAVNYRIPLPYILKINKITGYEHKPYIFI
                     PCGTISDLDRSLFLGTAFVSPLTTGKLSSNFGVRNDPIDHIHRFHAGVDIACPVNSPV
                     YASRDGIVIFAGYKGGYGNLVIIQHSRGYFTLYGHLNTIAVTKGNKVATGHVIAKSGN
                     TGRTTGPHLHFEIRKNYKPINPLLLLR"
     gene            complement(67012..68997)
                     /locus_tag="PLJ75_00330"
     CDS             complement(67012..68997)
                     /locus_tag="PLJ75_00330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD03698.1"
                     /translation="MGLLEKALQYKQEINKKGKETLIDTITGPADTELVLKEKNKSES
                     LDFQDDLLTELSREDLKTIEPVEDELFILPEDDKKTLMEEENTLHTVEEKKVSEYSDT
                     VTSINGKDEYLGPEDEPDLDVLLSAKQEDLTIPESSKSQNNTKFRIVEQTHNTMDESL
                     KDENDKEQTHSEAISSKPDKKFQDFVVLYEIGKEIVKSKSLNELYDTILFSVMGQIGS
                     ASSTLLVLNPDEENSLIVADSRGITIKNKNIKFDTSRGLFKILMQSKGVADIEKYKQQ
                     TQYRDEYYQLIAIDARLFVPLRYNAIVFGALVLGDKITIGDYNEEEIDFIEQVSEFAA
                     IAYEKVKKIEYLESDIQRYLDDKNFWQSIEEYKQSLRKYPQHDYFKITVQNMMKANGI
                     LSFAVFVKNNKTMDFSLLLTEEDDVLLLNESVSIIKKESPFLDYIASLHLPTIIEDFD
                     RNKAIKEVFTESCMRKMTVFWIYPFQISDELLGFVCVFRLSDYQLKDLQFDNFRSLSD
                     VILTHYWSSRLIEYQYTNYIDVIVPVFNRISKELNNASKLSIPLTIVLFTIKNLKRYY
                     IVQGFNKTKELIDKVEDIIHNRLSEGDFTAKIDRHKFLIVLPGKNKKFAVPFANALKN
                     EIVQQFSQKELQLLVTFLMAEYPEDGNDLYSLLDAID"
     gene            complement(69003..71384)
                     /locus_tag="PLJ75_00335"
     CDS             complement(69003..71384)
                     /locus_tag="PLJ75_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03699.1"
                     /translation="MSQKSKDIVHELEEIADIPETIIDISEEDIVIDLPEEEKPSGEF
                     EEITDLTDSTSSTITIEDENPSLQLHEMDDDVINITLNVDAINEVRVNNKSSKPAVIK
                     PVQKAKLFEDIPEPMESFINGMPSLNVKEEYVHTAYDDENREVKGKIYFDNAADALVI
                     EDIVEEDEKLSQKSESQKLSQKTIEQNKNIPTDVEQHASSAMSDDKESQKGNSLRSDV
                     ITIEIPPEVRTKIEKSKIEDFDFINLDEAEQIASEEILILSEDDLIEELEEYDLIPVD
                     TSNAVNNITAKEKSKNDNINSEDYVRKEVQGKDDTLEVSIMGDVKKQKVEHDASQTSG
                     IIHEKAGKISKNETTIIKEGNTSAIIVDHETRGKVHIQSTNNVTKNDKTVESIIEDVS
                     EILIDDDKAMGSDATISTIKPVAIETKHAEESFKVEPIIASLSGDSVKEVTAEQPAKP
                     FETIPDDIQKMLGGNQRIVIVDDAGVGGTELPVADKSIDEIDNLVSGMIEITEGEAKI
                     LTEAGFKEEEEYIAPILTGTIPTFQDKMVEFGEEYKFSDDDIVFVDNAIVTDEYDKYL
                     KVIDEYYETKTVKKGSSTSELFGLSNEELAFFYDVMFEKEYETVKASKTFKDVAVGRD
                     QPQRDETLIRQFKYIESKPKSFSEKEKRSIEENIESPMAVIIEENIDDIVERLKTIKP
                     QIDINNLLSTAPDKEVNGMLENVSESFEKSEGINVEQQKYTNITDQVIVLESKDDVNR
                     FVDTLPVEKRESIRKLLKYLDSLFDKLPEEVIKNFANSEYFDLYVKVLNELGV"
     gene            71864..72433
                     /locus_tag="PLJ75_00340"
     CDS             71864..72433
                     /locus_tag="PLJ75_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPD03700.1"
                     /translation="MKLSNKLACLVVAMVLVPSVSFAIVDFGVYGGYTFAGKIDSPTI
                     DDAETDGWQYGFIGHLNKSIIPMILSMGIGGFYQRSPLEYSDSGKDYDLTKTMYGIDA
                     IATLELPIIHPYLRAGIAINEKIKVDTPSGTYTDEKKFNSYYFGLGAAFTVFPFIQIF
                     GEYLYNYSKQEHDSTLHSNSINIGARLNI"
     gene            complement(72621..75242)
                     /locus_tag="PLJ75_00345"
     CDS             complement(72621..75242)
                     /locus_tag="PLJ75_00345"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPD03701.1"
                     /translation="MKKNRDGIITSGIYDGLIYEHLNPVVEKLALVLFTTVDFDDESK
                     SRIEQCMSRGHVVYASFQSSNLSLLILYNILKRNKYPLPVFALEYNPFWFQDMGYIAR
                     RLWQYFSSTILRKQYEYVLDTDYVESLIRDNKSILFSLLSKQYFLKRYTQVKYDSLRY
                     LIDVQKRIDEPIFLLPQMIFWNRNPERSGSPLGPLYSIISSMATGDKTLLFAWLSIMK
                     SATPPFVRIASPVNLKEVIEKAKVDDTRQLAVMLRNKLLDDYYHEKRTILGPVLKSQH
                     EMMERVLYHPNVENEIKRLVAEANISEAKLRRDAYKYFKEIAANFSIIYIRLFEIALD
                     WMFRKIFDGIRYDAKAIEEIRQAASNGPLVLTPCHKSHLDYLLLSYVFYKNKMCPPHI
                     AAGVNLSFFPMGTIFRHSGAFFMRRTFKGLRLYPTIFKQYIKTIISEGYTVEFFIEGG
                     RSRTGKITFPKLGFLSYITEAIDEGYNTDVVFVPISINYDRILEESSYVQELKGKEKE
                     KESVVSLMQSSKLLNRKYGMVYINFNKTFTLKELKEKLNPVNITEAVAMNIVRRINDV
                     VTITPFSLTTTAILMSSVKGFSRDMVKANFESLYDYCEYKNYSMSESLKDKSYFDEII
                     DYVIQSFKEDKILETLKIVGEGKKLEAMPDMYVLPEENRPRMIFYKNSIVHYFLPLTF
                     SSLALRVLHKNGQSSFDSFRSEYVYIRDLFMKEFIYSEDMLDDVMQSIQSVVTYMQMT
                     GFATVTQKEITINTDREKDAKLRFFGRILQEYLESYYVVCATLLPAKHTRIQKKDLLT
                     DIRKNGVRMYHTGEILLSESLSLPNYTNALSLLRSEKALSEKQMGRKNIVIEIINQKT
                     LEILFEKIKIYLEGIRA"
     assembly_gap    75703..75802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            75930..76004
                     /locus_tag="PLJ75_00350"
     tRNA            75930..76004
                     /locus_tag="PLJ75_00350"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:75965..75967,aa:Pro,seq:cgg)
     gene            76034..76444
                     /locus_tag="PLJ75_00355"
     CDS             76034..76444
                     /locus_tag="PLJ75_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD03702.1"
                     /translation="MLHSKMTLLKAKYPTIKEIRKSIIDDIMNQLTINKIHVALDNLE
                     LYLIIDEAITNAMEHGNTWDNNKYVHVAVQADEKQLYVTITDEGKGFKHPDSHDTSIL
                     QPRGRGIFIMKQFAKIVWNKKGNSITMAIPTVSR"
     gene            76473..76790
                     /locus_tag="PLJ75_00360"
     CDS             76473..76790
                     /locus_tag="PLJ75_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03703.1"
                     /translation="MKRYGTTPNRLSNATQKRVVYTGNNIKRIGIIIAMAIVMVIVAT
                     AIIRDCNQQKNVSAIKSANIEPTVKKHDNKRKQTTHKNAAIPYEAPRDSSPPQTNLNI
                     ITD"
     gene            complement(76822..79200)
                     /locus_tag="PLJ75_00365"
     CDS             complement(76822..79200)
                     /locus_tag="PLJ75_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03704.1"
                     /translation="MKRFFTYLFELTFRKPKLRYFVWGVIVFLLIYGSFYLFYQEVVT
                     FPVGWEKSVIITPYTLKIKSPYAVSKGNVIVAAFEGNEKNESGVYITVSLNAGHDFLP
                     PYKLDTIDPAVAIRPHVAISNNGHIACTWHNLINNRSAIMLTISSDLGATWTKPVALS
                     SISDQSMLSQVYYDASGGLHLFFHGTVKGRVTLYHAYSVDELNFSQLVAITDISSEYR
                     GIFFPAISFSNNAIYIVMQGKTLSGGLLSDDLFFLYSTNNGKSFSNPKKITTSPANDS
                     SPSIILHNDVIYCVYQNNENRQWGIKLLRGFDKGKNWETQPFNVTTVNVNCFQPRVVA
                     ASNDDLLIAWYDARERYEAVFAKKFSLIDNTFTSEVRISDPNVNSNDQFPVLSGQRIL
                     LFWNSNGRLIAKFSDISVLPPRIYSVTHPEGRWTRSTTAILEWVPPVDESGISGYAVI
                     YNELPSFDPAVQNIEGPIHKYIVTDLKDGITYFHIRAIDRAGNYSRTIHYPLMVSSTP
                     LPMPTVSSPTHPEGTQVNNPSPVLQWTIDEDERLKGFYYSIAKDAIKEPSDFTTANTV
                     GFNNLPQGRYFFSLRPIDKTNMKGRIATYEIVIGNVEKVDAEYYEKLAKGIVQPEQKP
                     IENLVAQKIYPSVAINIPFQEIYATQFYRFQLVPHHIPTQAVDGFSVIVAQSKEVPPF
                     FVSHARGEVTLTNVKPGKYVIAARMRYSDYQNGERVYRWTKPCYRELMVKSAHTSPLM
                     HYADNLYVMIKDRQLITAIYSVGLLAIVLIVGMSARISFAFQKLLYRVSLLF"
     gene            complement(79219..80940)
                     /locus_tag="PLJ75_00370"
     CDS             complement(79219..80940)
                     /locus_tag="PLJ75_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD03705.1"
                     /translation="MYEKKPWLKFYGDVPHTLEYPKVTMYEALMNTVSRKPNAIAWDF
                     MGHTATYKEFSKLIDQCARGLYALGLREGDTITISMPTVPQGIICFYAVNKIGAVASM
                     IHPLSPPKEIEFYLTISNSKFALTIDAFYNAFNEIMDKTPCKTLILAKISDYLPALKA
                     LGFKLTKGRKIPKVPQDPRVVWWNDVMKQSSGDVPKSNMKTDDMAVILYSGGTTGMPK
                     GIMLSNMNFISEGKQVAAWGRMSEGDAVLAILPIFHGFGLGVCVNAFFMGGGKSILVP
                     QFDAVVIAKLIKKTKPNFLIGVPTLFEALSKSKVIQSADLSCIKAAFSGADTLPRVVK
                     DEFERVVAQQGGNAKLREGYGLTESVTAIMAMPMNEYREGSIGIPFPDMMAKIVKTGT
                     IQEAPWGEEGEICIHGPAVMIGYLNQPEETAKTLRKHDDGLVWLHTGDIGTMDEDGFF
                     YFKLRLKRMIKSSGFNVYPTQVEDVLRQHPAVDQACVIGVPDKAQVERVKAFVVLKPE
                     YKDKKSDDLVQELIKHCRDHLIKWSCPREIDFRDELPQTKVGKIAFNVLEAEEKEKLK
                     AKGEYTG"
     gene            complement(81129..82364)
                     /locus_tag="PLJ75_00375"
     CDS             complement(81129..82364)
                     /locus_tag="PLJ75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPD03706.1"
                     /translation="MSGEMQDKKDNRIKSVKRMKEIMTNYYIDAKTAAQNNKKVGWIT
                     SGGPVEPLLVMDVIPVYPENHGAMIGAAKMGVQLCEKSEEMGYCRDLCSYARSDIAAA
                     PINGGPIGGLPKPDFLICCNNICGTVLKWYEVQARYFKVPLFIFDTPFCHKGITQEAK
                     DYVGAQIVEYITFLEEHCGKKFDYDKAKEVGKLSIQGQRLWQAVLDTTMHKPAPMSAF
                     DAFFHLALIVTLRGTQIAVDYYTELLQEMKERNAHGISAIPNEKYRLLWDNLPVWYKT
                     KWLSETFASHDACLVADTYTTAWCRQLDYLDENDFLKSMIEGYTAIYLNIGVDQMADA
                     VIGMVDKYDVDGLVMHSNRSCKPYSLGQYDIQRIVQQKRNIPSLIIEADMLDERNFSE
                     SQILTRIEAFMEIIKNKRR"
     gene            complement(82379..83485)
                     /locus_tag="PLJ75_00380"
     CDS             complement(82379..83485)
                     /locus_tag="PLJ75_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPD03707.1"
                     /translation="MDIIKSFEDLNPYQYVASMKKATGKPVLAYMCSYTPEEIIYAAD
                     IHPFRIFGMVDAIAKADAYLQVYSCSLVRGALEDALSGKLSFVDGAVFPHTCDSIQRL
                     SDVWRLNAGMKHHFDVVWAVKLNTDSAKKYMYDVLAKFVKELQDAFGITITSEKLEKA
                     ISTYNEIRSLLKTIWQLKSDNKSTLNGSQMNALIKAAMVWDRDELAGMLKNLIPSIEL
                     SPKNEKAKRIMLIGGICNHPDIYTYIEDSGGVVVWDDLCTGSRYFEGQIATDSNPLQA
                     IANRYYERNICPAKHRDLNTRVETITSMVREHNVQGAIFLYLKFCDPHSFDYPYIKEA
                     LDTMGIPSTLLELELQLPSEGQMKTRLEAFIDMI"
     gene            83897..84679
                     /locus_tag="PLJ75_00385"
     CDS             83897..84679
                     /locus_tag="PLJ75_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPD03708.1"
                     /translation="MKTKKVKLIQSIAKAHDILSFFVHDKRTIGISEFAKKLSLPKTT
                     IQSIVKTLCYLGYLEKDASTGKYMLGPYVFQLGMKYATNLDIVSIGRVWAERLCFQYR
                     QPVNIGMLVGNKVVVVLRVEPENRFMTYPQTGSVIPMHTSCIGKLLCAYTDNYRLQEL
                     LENYEFQALTKNSINSREDFLKELQSVRSMGISFDKQESIVGLAGIGGPVFNYTGQII
                     AAFAITGDADFIEKYRHDIITTVKDTSLQLSLQLGYIPANHQ"
     gene            complement(84795..85328)
                     /locus_tag="PLJ75_00390"
     CDS             complement(84795..85328)
                     /locus_tag="PLJ75_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03709.1"
                     /translation="MIKRLVFVVYCMIVVTNGYAVDINTIFNDEQIAFIKTATDMVQA
                     SLGYDDIVEVVYVTFWNNQPVDNKKVAAFNKYIAQKYTEKPDTVVYVYERLLRSLYMI
                     EYMAQCAREEKQWKQYYYYTENLLPDTKRFLDLLKNAVVAADPQYAAQADSREKAIKN
                     FAIDLVKYNQNLYGGGF"
     gene            complement(85408..85638)
                     /locus_tag="PLJ75_00395"
     CDS             complement(85408..85638)
                     /locus_tag="PLJ75_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03710.1"
                     /translation="MIYKYKTFDEAQRALWNFNPDAKYYEQVRQLFLLSSKLYPIQCR
                     RGIFLFKTIEEANEFRKQEELTQALKNITNSD"
     assembly_gap    85658..85757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(85912..>86838)
                     /locus_tag="PLJ75_00400"
     CDS             complement(85912..>86838)
                     /locus_tag="PLJ75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HPD03711.1"
                     /translation="AADSGADLVKFQIINAELLYSVYTRSLINNQPPHYDDSMITFFK
                     QFELTQSDYTKLQQIAKEDGVIFFASCFDLPSFAMLEQLQVPLYKIASSEITNVPLIK
                     NIAATRKPVLCSTGISHSYEIDHAIKLLQERGVNNIVLLHCISLYPVPPHAINLLRIQ
                     HLQHKYGVPVGFSDHTNDNRASMMAVASGALVFEKHFKLSNDHDCVDKDVSLSPEAFK
                     RYVDDIHMAYTMMGDGAIDYHDAEADVARSARRSIFAAQDIKKGTIVTEEHITLKRPG
                     VGLSPLYLDKIVGKKIKRDIIKDMPLFENDMD"
BASE COUNT        27055 a        17619 c        13479 g        28485 t
ORIGIN      
        1 ggcataaagt ttattgcacc ctgttgcagc cagctataag gcgatgattt aacaatatat
       61 ttaaccgatg ctttgtttgc tttcaacaat ccctcatcag cttccaatga tgaagctttt
      121 aaaaatacaa tcttctttaa atatttagta atctcacagt catcagataa tccttcatca
      181 tctatcattg gtgcaaagta tatggtaagc tcgcccaaac cccgctgttg agcaataaag
      241 cactgtttat ccatgactat atcttttctt attttaattc cataatgtgc cagcatcccc
      301 tctaaaccag taataactgg caatgcctgc ggaggtcttt gaaacatctg catcccttca
      361 gcctgttgtt caacatatga gtcaagcaac agtattgcat tgccgccacg catcaaaaat
      421 tgatcaatct tgaaaagttc atattcgctg tacatggaac gagggccatt gattattatg
      481 gtgataacat catcaggtat ttcatccttt gtaatgtcaa cagtaacaag gtcatacata
      541 tccgacagca tcttcctgaa ctgtgcagcg ccgttttgtt catcattaat atccttttca
      601 ccatgtccgg taatatagca aacttttgga ttgacactga taaggttatc gatagcagcg
      661 tttaacatat cctcaagacg tgataaatcg ccaatggcat attgtccaaa gattgaacgg
      721 gtaagtatct gtaccgtttc aaacttattg ttatgctcaa caactatccc aaccataccc
      781 tgtcctggag gtatggctct accttccatg gtggtaaatt gcggccattt taacgttggc
      841 agcccataca tctgtgctat ggtcagcgcc tgtggattat tgaccggatc aacaaagcgg
      901 tattggattt tattgtaatt acgtatatta catttttgca cctgagcata cacctgctca
      961 tttaaattct gcattccagg gataggaaga ttgctgctgg catataacgt aacagtaatg
     1021 ggatctttaa tctttaaaag agcatctatt ttcccattca ttttttttat cagggttgtt
     1081 atgcggtact caagaccttc aggctcggtg attgaatcta tcttttctat tatatcacca
     1141 tggatgatag caagccccat agaagcattt ctaaatttta cctgatcatt gcgaatctca
     1201 cgtatctgaa caggatatat cccaaagtca cccgcagcat ctttctgttt ttcaacatca
     1261 ataaattcat aatggaaata cctgttgcct gcattatcat actcaaccat caaatcctgc
     1321 aaataacgat aaacagtatt atacggcgcc ggcaggtctt tgttaaagaa aatctttact
     1381 gtcagaggtt cttcaagtgt tgaaaccact gctttgctga tgggtgatag tgaataaacg
     1441 ctattttttg tgaggtctaa ccgtacatag agggttaaac caacaaggtt aatcaaaatg
     1501 atgataattc catataaaat aaaatgtgag tagctttttt gtttttgcgg tttatcaggt
     1561 gctttcccta ttttttgcat tatgcgcctg agtgctgcat caaacctatc aacgattttg
     1621 ttactgtatt ttttgatatt tcccatgttc atagcctatc ttctcacctc cagctggtat
     1681 atgcttgcca tagaacttat gatgataacc gaagcaaaat agataatatt gcgcgtatca
     1741 ataatacccc gtgcaatgtt ttgaaaatga taatctgcgc tgatatattc aaataaagaa
     1801 accaatttgg gtgacacaaa atataaaaat tttgttatga gcgatagtac tatgcaaata
     1861 gcaaacccta tgataaacgc tactatctga tttctggtta acgatgaagc aaacacgcct
     1921 attgacgcat atgctgctgc aaggatgata gcaccaataa atcctccaat tatagggcct
     1981 atatcaggtg aacccacaaa agcaaccgat actgcgtaaa acagagtggg taataccata
     2041 ataccggcaa aaatcgtagc tgctaaaatt ttaccggtaa caatctgcac agtggaaagt
     2101 ggtaatgtca taagcatttc aaatgaaccg gtattccgct cttcggcaaa aaggcgcatg
     2161 gtaattgcag ggataacaaa ggcaaataaa agcggcaata gttcaaaata tgcccgcagt
     2221 tcagcctgtc catataaaaa aaatgtggaa aaaaacaaaa agccggcaat caccagaaaa
     2281 actgaaatga caatatagcc aataggtgtt gagaaaaaac cagccaattc tttttttgct
     2341 actaactgtg cgcccttcat actatcctta aactgttgca tgccaatccc tccttaagct
     2401 cctaccgtta attcacggaa aacatgttcc agacttctat attcacgatt catttcgtat
     2461 aatacccagc cattatcttt tatgcagtta aaaattgcag ggcgtgcatc tatatccgaa
     2521 ccagttatca cctgtacatg gcacagcccc tcctcatcgt gtatatggtt cacattatta
     2581 ataccaggta tcgcacacaa tgctgtcttt aactggtcaa atgaagcgcc acctgccaga
     2641 atatttattc ggatatcctt tcctttggcg ctcttcagct cgcttgttaa atcatcagca
     2701 actatcaccc ccttatcaat gataataact ctgctacacg ttgcctcaac ctcctgcaag
     2761 atgtgggttg atagaataac cgtcttttcc ctgcccaact ccttgataag gtcacggatt
     2821 tcaattatct ggttgggatc aagcccatta gttggttcat caagtatcaa aatttcaggg
     2881 tcatgcaaaa tagcctgcgc cagccctacc cgctgtttat accctttgga aagctcattg
     2941 atatttttgt gcattacctc ggttattccg cattgtatag caacatccgt tatcctttcg
     3001 acatcattga tctctctgat atcagcaata aattgtaaaa agtcatatac catcatatca
     3061 ggataaagcg gagccgattc gggaagatag ccaatacgct tttttatctc aattggattt
     3121 tcatccacca gataatcatc gatacttacc attcccgatg ttggttgaag atagcaggtt
     3181 aaaatccgca acgtggtggt tttaccggca ccgttgggac ctaaaagtcc agtaacctca
     3241 cctttacgaa tggtaaatga tatgccgtca actgctttca catcgccata atgtttaaca
     3301 agattctgta cgtttatcat gcagggcatt cctcctgaaa atagttaatc atcagtttta
     3361 tcctgaagcg atagctgctg ttaatgccac ctgatgatta taataaaagt ttttattgca
     3421 aaggtcattt ttaaaagctt caataacctt ttatgataaa gttatcatga ttattcaaaa
     3481 acattacatt gtaacattgt attgtaatat taagctcaag ctttgtatgt agtatgaaaa
     3541 aatcaattat aatttgttac acactatttt atatacgccc ataatcatcc tcaatacgct
     3601 ctatatcatc ttcaccaaaa tattcaccaa gctgaacctc tatatacacc acattttcat
     3661 caccggtatt ttccacccgg tgcactacat taaaatctat atcaacacat ttcccgacat
     3721 caacggggat gttatctgta tcaagggtta ccacccccct gccatgtata aaaaaccaat
     3781 gctctttgcg ccgtgcatga cgctgaaggc ttagccgttt atggggatac acggttatcc
     3841 gttttacctt gtgagtttca gtgtccgata gtacctcaaa aaaaccccat ggcctgtgct
     3901 cagaatatac agtacgttca tccataataa tcacccatgt ttttccataa agcgatgcat
     3961 aaattcaacc agggtttgca ccccttctac aggcattgca ttgtatagag aggcacgtaa
     4021 tcctcctacc gagcggtgac cctttaatcc gcttaaacct tctttttcgg catcatcgac
     4081 aaatttattt tctaattgct catcgggcag atgccacaca acattcatgg cagaacggct
     4141 gtttttatca acaggactgg tatagtatcc gccactgttg tcaattgcat catagagcat
     4201 ggcagctttc cggtcacgcc gtttttctat ctccggcaat ccccctattc ccttaatcca
     4261 gtgcaaggta aggttggtca tccatacggt aaatgtggga ggggtgttgt aaagtgaatt
     4321 ttcctttgca tgcagatcat accgtaaata ggcaggtatg tctttgcggg ctttttctac
     4381 caatgatttt ttcataatca caagcgccat ccctgctgga gctaaattct tctgtgtgcc
     4441 cgcaaagagc atggaaacct tattccaatc aaccgctcgt gacatgatat cagatgacat
     4501 atcaacaatc aagggggtgt tccccgtatc cggaagctgt tgccactgta tgccatctat
     4561 cgtttcattg gaacatatat gtacatatac agcatcatca tgcaatgaca attcatcaat
     4621 ggaagggata cgtcgatatt tttcaccagc gccatcccag attattcgtg cattcccaat
     4681 aagccttgca tcctggtatg ctttttttgc ccatgtaccg gttataacaa aatctgcact
     4741 gctgtcagcc ataagcaatg ccatgggcac cataccaaac tgcagtgttg cgccgccctg
     4801 aataaacaaa atatcgtagt catcgggcac ctgtagcatt tccctgacaa gaactatcgc
     4861 ttcattatgc acctcatcat agattttacc tctatggctc atctctataa gcgacatccc
     4921 ggtatttcta taatcaacaa gctggcgtga agcttcttcc aatacctcaa ctggcaatat
     4981 cgatggacct gctgaaaaat taaaaatccg ccccattctg ctccccttaa caataatttt
     5041 aactttatac ttgaaagaaa agctattgtt ggtatactgc caaaaaaatc aagtaaataa
     5101 atgaaaacac tctatgacag gtaaacttta tatcatagca acaccaatcg gtaatttaga
     5161 ggatatcacc ctgcgtgcgc tccgtatttt aagagaagat accgacatcg tttattgcga
     5221 agatacccgt caaacaagca aacttttaca gcattacggc atcagcatcc ccgcacaatc
     5281 attgcatgcc cattcaggca aaacaaaatt ggaatacatt ataagccagt tgctatcagg
     5341 aaaaaatatt gcctattgta ccgactcggg cacacccgca atatctgacc cgggcggtga
     5401 gctggtagca ttagcccgcc ataacaacat accggtaatc cccattccgg gggcatcagc
     5461 cgttacagca ctggtatccg tttgtggttt cccctcaagc tctttttatt ttgctggctt
     5521 tttaagtaaa aaacagggga agcgcaaaaa gcagttgcaa cagttagggc aattgcaggc
     5581 gatagttatt gtatatgaat ctccttaccg gatagtcaaa ttattgtacg atattgccga
     5641 aataatgccg caggcaacca tcctgattgg cagggagatg accaaacatt ttgaggaatt
     5701 tatatacttc accgctgcag aggttgaatc aataattccc tctctccatc aaaaaggcga
     5761 gttcgttata gcactataca atgaagcatc taaacattga gcttgcaata ccttttaaag
     5821 cataaatagt gatgaaatag ctatggttaa ctatttttga atggctcgca taccttacca
     5881 ttttatgaaa taattacttg attaatttat agggagagtt tatatttata ccattaaatg
     5941 attaagtttt tataatgata tgccgatagc tactatagat taaaaaaggg tgattgcaat
     6001 ggttatagac aaaataggaa atatcaagaa tatcgttgaa cctaagaatt ctaaatctgt
     6061 gggaaaaact atatccacaa ataaaagcga ttctgttgaa atttccagcg aagctaagaa
     6121 agctgccgaa caatctaaaa ttgctcatgt tgtaaagcaa acccctgata tcagagccga
     6181 acgagttaga gaaattaaag aaatgattgc caatggtact tataaatttg atgatgatcg
     6241 tatcatagaa aaagttgctg aaaaaattgc tatgcagctt ttgcacaaat aataacttcc
     6301 tttttaaggt taaagctgat ggccggcata attgataaaa aagcggaatc attattccgt
     6361 ttttttatgt ctaaaacata tggtgaaact attgaaggat tgtggcctga tctttatatt
     6421 cctacggcaa tatatgcaca gcccgatggc atatcgttac ttccttctat agttcagctt
     6481 tttggatcac gtgttttgtt tataacaagt acggttgatt tcaaccagta taaccatctc
     6541 ttcatgtcaa tctccagaag cttacaaagc aataacattc cttttattgt atacgatgaa
     6601 ttccctgaaa gtaacaccga ctatattgat tctgcagttt attttgctcg taaaacccat
     6661 tgtaatctta tcattggttt agggggaatt gattctatca atgctgctaa aacagtggcg
     6721 ctgttaacac ctaactatct ctttgcccat gaactatttt cataccctca cctttatgag
     6781 ccaatacctt ttataagcat acccactcat cctctttttg gttttgaaat aacgccatgc
     6841 accattatta gtgatattca tgatggtata aaaaaagcat ttttccataa ctacattttc
     6901 ccaaaagcta tcatcataga tcccgaatta tcagtaagtg ttgaggaaga tacaatagct
     6961 aaatatacac tgggcactct ggcaattgcc acagaatcgg ttatatcaaa aaacatcaac
     7021 aatatcacca ataccctctc cctcagaact attgaccttg tgttcaaaaa cctgcatgta
     7081 gcattacaag aaagtgataa tgagacggca aaacttaatt tattgatggc ttcccttatg
     7141 gcaggaatag cttttagcag tgctcagctt tctgtttcgc tggcaatatc acttgcgctt
     7201 gcttcattga cacagattga tattgcatca tatatgagtg ttatgctacc gcatatcatg
     7261 gaatataacc tgacctccgc acctggtaaa tatgtacaga tgtccaaagt aatggatgaa
     7321 gatgtgcgcg acatcaccgt tattgaagcg gctatcaaag ctgttgaagg tgtacgaaag
     7381 ttagcgcttg atattgatat tccacaacgg ttgtcgcaat ttgatgtcaa taaaacaatc
     7441 ttttcaaagg ttgcagagat aacactgagc taccccttta tacaaaatgc tccgcgagtg
     7501 ctcaaccgtg atgaggttga aacaatcctg attgctgcat actaatctat gaactatcga
     7561 cctattaaaa aactcctcca gcagtttaaa gatgatacca ttaccatgga tgatttcatc
     7621 agggaattag agcaccttaa tgttgaagat cttgaacata caacattgga ccaccagcga
     7681 aaagcacgcc ttggcttccc ggaggtaatc ttttgccaga acaaaacacc tcagcaggtg
     7741 gcagacattg ccggagctat cgcaaagcaa aactatagct ttttagccac ccgtgcgtca
     7801 ttggcccatt tcaaagcggt aaaacgttac attaaaaaag cacagtacaa cacattggga
     7861 aaaacaatat catttagtgc acaaaccatg catcataaac cacacaggct tttgataatt
     7921 actgccggta cctcggatat tcccgttgca gaagaggcaa aggaaacagc actggtgatg
     7981 ggactaacgg tagaaacaat ctatgacatt ggggttgcag gtattcacag ggtatttcac
     8041 aattgggaca aaatccatga agcaaccatc attatagtga ttgccggtat ggagggagcg
     8101 ctgccatcgg ttattggcgg attggcaccc tgccccgtca ttgccgtacc aacctcggtt
     8161 ggttatggtg catcatttca gggaattgct gcgcttctcg ggatgctcaa ctcatgcgca
     8221 ccgggaatca gcgttgtcaa tattgataac ggttttggcg cggtatattg cgcataccgt
     8281 attattaaaa acctgccgga tacccaatga aaacacttta ctgcgatata cagcttggtg
     8341 cttcaggtga catgcttatt ggtgcactgc ttgacgcatg ccttcccgtt gatgtattgc
     8401 aacaacagct tgcatcgtta ccgtttactg gctttgcgat agctcctgta aaacaaaagc
     8461 ggtatggtat aaccggcacg caggcaggga ttactgctgt ggaagacacc catcaccgag
     8521 attttactgc cataaaaaaa ataattgcaa ccacctcatg ctccgacacc gtgaagaaca
     8581 atgcaacagc catctttgaa acactggcta ctgctgaaag cgaggtccat ggcatagcaa
     8641 aagacgaagt tcacttccac gaggttggtg cagttgacag catcattgat atactggcat
     8701 tttgcattgc gatagagtat ttaaacgttg atgcaatcta ttataataat tttgcatttg
     8761 gcagtggcac cattacaaca cagcatggtg ttattccgct accagctcct gctacggtaa
     8821 ggcttaccca caacaaacag gttacgttta ccttgaacaa tggagagctt accacgccaa
     8881 ccgctgctgc catactggtt acactgggta cccagcaacc ttctttcagc ggctgtattg
     8941 ctgccaccgg cattggattt ggcacgcgcg attacggatt cccgggttat acccgaatca
     9001 tactattgaa tgaaatacat gaagaagaaa gcagctacaa gcatgaactg ttgaatgtca
     9061 tagaatgtaa tatcgatgat atgaatcctg aactggtgcc ttttgttgct gatgcacttc
     9121 tggaagcggg tgcactggat gtgtactacc atcaggttat catgaaaaaa ggccgtcccg
     9181 gtgttgtact ctgggcactg tgtgccatag aaaaaactga tatagtacaa aaaattattt
     9241 tttcgcaaac aacaacattg ggggtcagag cctattcagt caaccgttct tcgttatcac
     9301 ggcaggtgca gacagtgacc gtatatggag aaaagattac cataaaacta accagccggt
     9361 atcatgagct atcgccgaaa cctgaatttg aagattgtaa acggttagca cttaaaacag
     9421 gcaaaacggt gcgtactatc atggaagagg ctctgaagga atactacaag caccaaacat
     9481 tataaattag tcaattcatc tgctgaaaat atcctgtcaa tatcaagtat gatgacaaac
     9541 ttgttttcaa ccttccctat cccaatgata aatcggcctg atatgccttt gacaataggt
     9601 ggtggtggtt caatgtcttt ttgctctaac tttacaacat gacttacctg atctattgca
     9661 agccctatgc gctttccctt aatatccact acaatagcac gtttgttctg cgcacttttt
     9721 tcagagattc caaaccgtac tcccagatca ataatgggta ttaccttgcc gcgtaaatca
     9781 ataacaccaa ggatatattc cgcagatttt ggcaatgggg taacttcagg aagttttaaa
     9841 atttcctgta cctttaatat ctctataccg tattcttctt tgccaacatt aaaacacact
     9901 atctgcatgg agggtgcaac actttctttt gtctgggttt tcataaacga ttccttgttt
     9961 aatggttaac tgtacctttt attctatagt agtttattta caattttttt caagttaaaa
    10021 atttattatt ttttttaatt acctttttat gacttctcat agtacaatgc aatatttcat
    10081 gtttcgttaa atctttgaaa attatgctgc taccataatc aatcggtata gcatggcttc
    10141 attgcaattt tttttatact gtatgattct taagtcaagg caaatatgtg atgtatgtac
    10201 aattactctg ttagcttttt ataggtgtgt tgcaactgtt caataccaat cttcccttca
    10261 tcaagatacc cctgtaagct tttaggcaat gaaccatttt tgacataaag ataccctgca
    10321 aatataataa acatggtgag cagtagcaca accatcttca tcaactgttt cataaatata
    10381 tagaacaaaa ctataaaact aagcaacgct actatagtca atataacacc ggtgtccatg
    10441 gttgtacctt taattatttt ttgccttttt tttatgccat gaaattgaac taattgccat
    10501 ccctattatg ataccgcaaa caaatgttac aaacagcata acaataagtg gaagcgttac
    10561 ttcccaatga aacaatgaaa atattactgg ctgaacattt tgtacaacga ttatcaaaaa
    10621 tagcagtaaa ataattaaaa ttatgattat ttttggtcgc attatattct ctcaacattt
    10681 ataataatga catatactat tttccaatag tacaaaatat acaataaaaa aataataggt
    10741 acgatagttc cctgtataat tatcgctata attgcaacag aatataatat aatcaaacat
    10801 tttattgtag cctgcaaaaa acaatcagct tttttcctga aaataacatt attgtatcat
    10861 tgtcattacg agggacgcag tcccgaaaca tctattcttt cataaaacgt tgcgattctt
    10921 cactatcgct caggatgacg tcctcgtttc aggtcatatt tataaaaaac tcatcataag
    10981 ttcataaaat atttaccatg aagagccaat tttataatta gatttatgca attaatactg
    11041 aagaggagtt atgaaaataa aaaatacatt tgatgaacac attaattcac ttattaatag
    11101 ttttgtcaat gaacaaaaag taagatttcc gcaagacgag attttaacta tagaccttca
    11161 ctgtcatgat tacaacagca atgttcctga tgaattgctt ggaagaattt taggtatccc
    11221 tgaaacatgg ctatcaactg atgagctgat tgctactctt aaaaatcacg gctgtgatac
    11281 ctttacggtg accaatcata acaatgcgcg ctcatgcttt gaacttctag ataaagggtt
    11341 tgatatagta gttggtgcag aatttagctg tagtgttccg gactatggtg ttggcatcca
    11401 tgttcttacc tatggattta catccgaaca ggagataaaa cttaataaat atcgaaatga
    11461 catctataaa tttctggatt atacatcaca aaatgacata cccactatat gggctcatcc
    11521 gctgtatcat tataaatcca gaggcatccc cccaatggat ttctttgaca agatggcact
    11581 tttgttccaa cgatttgaag ttattaacgg tcaaagagat agctggcaga atatgcttgt
    11641 aaagcaatgg attgaatcat tgacaccaga aaaaattgaa cagctttcaa aaagatttgg
    11701 aataaaacca gacagatatt gcactcaacc gtataaaaaa tatttttcag gcggttctga
    11761 tagtcatatg gggattttta ccggtctcac cggaacatat ctacatattc ctcatttaat
    11821 gaacagatta caccatgaaa aacgatcatc gttagcgctt gaggctataa aaagcggtac
    11881 catggcacca tttggaacac ataacgatag tgaaaaaatg gcaatatcat ttatagacta
    11941 tttttgccag attggaatga agatgaaaga tcctggtttg gcccatattc tattgcacaa
    12001 aggtgaaacc attgataaat tagcggcttt aacaatagtc aattcatttc aggaattaaa
    12061 gcgacacaaa acaacgctcc attttttaga gctttttcat caatcattga ccggtaaaac
    12121 attttcaaaa tcaaagcgtt tttatgttaa taaagactat aaaaaggtat ttgatatagc
    12181 gtatacatta tcacgttata ctaaaacccg taaaaaagaa gacataattt tttataatga
    12241 ttcaatatat gctatatata atgagcttgc tcaactattg atgaaacgag cacataagta
    12301 tatacataaa tttttgaacg atacaggttc cgctacaaat gaatttgaat catattttca
    12361 aaatattgaa ataccaactc atttcagaaa atatattgct ccttcaaaag gagcagatag
    12421 taatggtatt cctgaactaa accttccaaa gatttttgat gagctgtcat tcccctttct
    12481 gggttctgcg gttattgcat cagcattttt taccagtgcc agggttatgt acaaagcaag
    12541 accactgctc tatgaatttg cacaaataca taatacatta aagcaccctg aacgtacact
    12601 atggttaacc gatacattta atgaccccaa tggagttgct aatgttctgc agttgatgca
    12661 taaggaaata aagcgcaatg atctgccaat tgatattata acagtaagtg atacattaca
    12721 atcagatgag catctaatca tcataaaacc acttaaagaa tacaccattc cattttatca
    12781 acagcaaccg ttgcgaatac ctgatatatt gcagattcat aaaattttta aagaaggtga
    12841 atacaataaa attattgttt caactgaagg tcctatgggg ttggcagcat tatggttaaa
    12901 gtatgcttat tctgttccag catattttta tttacatact gactggatga tgtttgccga
    12961 gaaaacctta aaactaaaaa atgataactt agacaatttc agaagaattt tgcgtacatt
    13021 ctataaagca tttgacggta tatttgtatt gaacaacgag cataaaaaat ggttaaccgg
    13081 taaggatatg gggtttaact cctcaaaagt atttttaaca tctcactggg ttgaagaaat
    13141 ttttaaacct cacaaaacca ataaggaaga agtatttgga gtatccagca ccacgccggt
    13201 aatactattt tcaggacgat tatccgagga aaaaggcgtt atggatatac cggaaataat
    13261 gaatttagta aaacaaaaac atcctgaaac aaaaattgct tttgcaggaa ttggaccaaa
    13321 agaaacccaa ttgaaaacat tattgcctga tgccatcttc ctgggctggg ttgatcatac
    13381 cttgttacct gaagtatatt cagcagcaga tattcttgta ctgccctccc gctttgatac
    13441 ttttggctgt gttgtactgg aggcattatc atgcggattg ccagttattg catacaacaa
    13501 caagggaccc aaagatatta ttaacaatgg gctgaatggt tatctggtaa aaaatgaaac
    13561 cgaaatggcc caaaagataa tagaacttat aagcaatcct ctgttgctaa atctattccg
    13621 caatcaatcg ctgttgaggg catatgaatt taaacctgat accataatga aacaatttgt
    13681 caatgacctc aattcagcag cataataaac atcctctcca ttatgtatgg tggaaacatg
    13741 gtgttatata ccagatatat ccccgaagtt tttatgatgc aaatgatgat ggcataggtg
    13801 atttacctgg cataattaaa aagtttgatt atttgataga tcttggcatt gatgctatat
    13861 ggctatctcc cattaataca tcccctatgt atgattgcgg ttatgatgta agtgattatt
    13921 attctattga tccaattttt ggtacattaa aagatttcaa agaattattg gcgatagctc
    13981 atagcaacaa tattcatatt atcatggaca tggttttaaa ccatacttca caactgcatc
    14041 catggtttgt agaatcatca tcctcacgat acaacatgaa aaacgattgg tatatatggc
    14101 atgatggttt taacggcaaa cctcccaata actggaaatc tgcctatctt tccagtgcat
    14161 gggaaaaaaa tgaaatgcgc gggcaatatt atcttcattc atttttaaaa gaacaacctg
    14221 atttaaactg gcacaatcag caggtaaaac atgcaatgtt tgatatgatg cagtattggc
    14281 tggatctggg tgttgatgga tttagatttg atgttatcaa ctattgctat aaggataaga
    14341 atctgcgtaa caatccattt tcctttaatc cgtttaatga tcaaatagaa aaatataata
    14401 aaaacaggct ggagagttac attttagttc aagaaatcag gaaattaatt gataaatatg
    14461 acaatcgtat gacggttggc gaagtattca gctatccgcc tgggaatccc gcgctttcgg
    14521 ccagttattt gtgtaaaggg aaaggtttgc acctggcatt tgatttttca ttgttatatc
    14581 aaccttttga ttcacataaa atataccgat gcattaaaaa ctggtataat catattccag
    14641 aacattgctg gccatgcaat gtattgtcaa accatgatca gcagcgctac gctgataaat
    14701 ttggtcagga agacaatgat tcgattaaac gtttaatggc gctattactg ttaaccttat
    14761 gggggactcc atttatttat tatggggaag agataggaat gaaaaacact tcaatcccgt
    14821 tacgtaaaat tgtagatccc gctggtaaaa gattctggcc aatgtataaa gggagggatt
    14881 gtgcacgtac accaatgcaa tggtcagatg aagctaatgc tggctttagt catactatgt
    14941 gctggctccc cattgatagt aactatcgca caattaatgt aaaacatcaa ttaaaagacc
    15001 gttattcact tttaaattat tataaagatt tgatcaggtt gcgcaaaaag catcatgccc
    15061 ttacccatgg ttcatgggaa ccactgataa aagggaaaaa tggggttatt gcatatatac
    15121 gaaaagcggc ttctgaaacg ctttgtatac tattaaattt taacgatcat gcaacgcaca
    15181 tccatcatca ctatgaagcg caatggaaag ttttgttttc tacgcatcgt agccgctttg
    15241 agcattttgc agatttacga attaattgct atccttttga ggcaactata ctacaaaaga
    15301 ttggagattt atagcaacgt atgaataaaa tagtaaactt attccagaaa aaatcattat
    15361 cagataatac ttcattagct ttacctgtct caatcacaca catacctatc tggattatca
    15421 ttattggcat agtactggca ctactgattt cgataagcgg ttttatgaat atatattttc
    15481 ttaatagctc ccatcaagag attgaaaaaa tcaatacatt aacctcatta gctttgtcaa
    15541 cacaaataga atttgaaaag caatttagca tgttgcacta tattttactg gagaaagacc
    15601 cattgcaata ccagacatat tttcataaat tttcatatca atatacaatt gttcagaacc
    15661 aactttttaa tctaaaactt cttttgaaag actttccaaa catacacaat aaaatgaatg
    15721 atatacagaa atatcataaa gacttaacag aattattaat tgatgagttg tttcaatata
    15781 aggataagtc agctactgtt gatgatgtat attcaataat taaaggcaaa gacataattg
    15841 caatagaatc gttaaacact attatctctg agattaacaa tattaattat gaaatcgcga
    15901 tgaaaacaaa gaaaaaatat tttattttat ccattgtttc gatatcatta ataagtattg
    15961 ttacattatc gctggttata gctattctgt atattactaa gttagagaaa aagtttatat
    16021 ttaacattgg caaacagctt tcatcttacc ttcccataca attggttcaa ttaatcctgc
    16081 gtcaggaaaa acaggaggtt gttcccatcc agaaaaaatt tatcactgtg tgttttactg
    16141 atatagaggg atttacaaaa ttaacagatg caatcgaacc agaaattgtt gctgaaatac
    16201 tgaatgatta cctatctgaa atgactgtgg ttgctcataa atacaatggg actgtggata
    16261 aatttttagg cgatggaatt atgatattat ttggagcacc caattctatg ccagcatcaa
    16321 tacaaattga aaatgctatg acaatggcag ttgaaatgca aacaatattt aaaatgctca
    16381 atgataaatg gaaaattaaa ataaacaata atatactgca tctgagaatt ggcatccatt
    16441 gtggcatgac aacagtaggt agttttggac ctccagaacg gttatcattt actgcaattg
    16501 gtaaaatagt taatatcgca tttcgcttgc agcagttatg caattcagat ggtatactca
    16561 ttagtgaaga tgtaaaaaat ataatgccgc agattcctgt taatgaagga atagcattaa
    16621 atgttaaagg aattgataag cctcttgaag tgtatagtat acaaagcaat taacaataga
    16681 gcattgtttt actcatattt tatgcatgca attgtcatgt catcctgctg aggataggta
    16741 tcacgaaaat tattaatatc aaataccata agatccaaaa acttctggac atctccatta
    16801 taattttctt tcataaattg ggtaattcct tgaacaccat aagcagcaca ttctttattg
    16861 cgctgttcaa tacatccatc agtgattgtt aatattgtat caccagaatt aagttttgtc
    16921 ttcccaaaat ttaatgtatt tattttttgt atacccatgg gaaaatttat ttcttttaat
    16981 gaaagtttaa agatagtatc gttacgatat aaatacgtca gcgaatgtcc aaaatcatag
    17041 agagttaata attctttatc ttcatcaaca tgcatgaaag cagaagttac aaatttttct
    17101 ccttcaaaat ttgtaaaaat ataattatta agatgaataa gaaatgtttc taaatcatgt
    17161 gtttcaaaat catatatgga catcatacca ttgagtaatg ttgtaatcat agaagcagca
    17221 attcccttcc ctgaaacatc acataccaca ataatgtgtt gattactttt tttaaatggt
    17281 attactatat agaaatcgcc accaacaccc tgcgccatta cattttttat cccaatagtt
    17341 aatcttttag tatgaacata ataatctttt tttaatatcc gctctataat tctttttgcc
    17401 tgttcaatgt cctgctggtt taactttgat aaaaatatta acatatccct ggttgaaaat
    17461 atgcctttaa aacaatcatt ttcatcttca agaagataat gatcctgaac acctgttaat
    17521 aaatcttttt caatagcttt tgcgcactca aaaatatgca tattataatt aaactgtttt
    17581 accttttgca ttacttcatc agcagtttta ttataattta attctctgcc aaaacgggtt
    17641 cctaaaatat caaaaagatc tttacgtatt attactccat acgatttttt atttttatca
    17701 actacagcaa cagcataaat tttatcattt cttgataact catctgctaa tattccaacc
    17761 tttgtatcag taaaaacata ataatttgaa cttatcagca tacctatagt aaactgtgta
    17821 caacttaatt cttcgatctt atgaacaatt ggttgacatg aaacaacttg actattatta
    17881 ttcatgaact aatatcctcc tggaaattat attatcatta tcattataat gaatactaat
    17941 aagtatggac acctctatca atagcattat tcaccgcaac cctgcccctc cccatggtaa
    18001 ggcgaggggg cattgtttcc atgtcattct gagggacgca gtcccgaaga atctattcgt
    18061 tcacaaaacg gcgagattct tcgctatcgc tcagcatgac atttcccgct caggataaaa
    18121 ccctgtacat tgtcatttca gctattactt taatgattct tcatataata aacaaatcat
    18181 tgtatataaa ataacagtta attgcttata aatattttat gaatatatca ctacttttta
    18241 atcaggagtg gtaaagctaa tctttatttg atgtagtatc cagatgtata caatactgaa
    18301 tacttgtttt ttaaaaaata ttttttataa aaatatgttg caaaaaataa ttgatctaat
    18361 ctatgattta ttttttaatt gagtattatc gtgggtgctt ttaatataac acaattttga
    18421 agatatgaat ttatatgaaa aaatctataa caaaaacaat gttgattatt aatttttttt
    18481 atatttcaat acttttacta tatactcaga catttgctga tatctgcgta gcaccatatc
    18541 aaagcccttc tggtttttct gttgatttgc ccgcatggga cagatccgaa atgatgaaac
    18601 aggatgcgct ggaatttata tgcgctcatt ctcaatatat ctatattaaa ataataaagc
    18661 taaaaaaccc tccggatgat attctgcact atatgtcctg gaagatatgg gataccgatc
    18721 ccaacgcaca cttcgttatt aaagataaat tagaagacgg cggaataatc atttcaacat
    18781 atcgcaacaa aacatattct cttcacaggg tgcgaatgat acagcaaaag gagtatacct
    18841 acatcattga atgcagtgct cctgaaaaaa gcttttacaa ctatgaaaaa tattttaata
    18901 gggtgtttaa aagctttacc ctgcaataat aacagtaatg atcaattccc ctgatgttca
    18961 tcacacagtt tacatcctgt acatgctatt tcattgtaaa ttcattggca atcatcaatc
    19021 ataattacag ttacgggata tgttgcaaca caattactac ttagtttgta tagactttca
    19081 tcataaatta tcttataaat gcatacgcaa ggaactttca aatttctctg caaattgtgc
    19141 tgcttttata ctaacacatt atttacaata actatcacat atgacatcat tatcaaccca
    19201 ttgctacctt aaaatatgca tgagtcatgc atatatgctt aaaatacaat aacagatacc
    19261 tgttttaata tcgcaaaaat caagagaata tcattatcag tgtttagcaa aaaaatataa
    19321 attaaaagta ctttgcaagt agatgttgta attcttcaat agtaaacggt ttgcttaatc
    19381 cattattaaa accatactcc tcagggttag ccaacacctg gtcatcatga tagccactca
    19441 taacaaaagc aggcacacca gtatcatgct gccgtataag ctgtattgcc tctgttccac
    19501 ccataccccc tttcacagta aggtcaaaaa ttaaggcatt gaatggagtt cctttttctt
    19561 tttcatgaag aaacaaatta actgcacttt gcccatcctc aactgccatt gcttcaaagc
    19621 ctaaatgttc cagaaagctt atcatcaatt tttgcatcat tgcatcatca tcaaggacaa
    19681 gtatcttccc tcccgttaat ttcttttcat acgttttatt tttttcttcc atttcaacct
    19741 tttgttgtgt agcaggtaga taaatataaa aagttgtgcc ctgccccggt atggaatcaa
    19801 cctctatagt gccattatgg cgtgctatta ttgaatagcc tgtagccaat cccaatccat
    19861 gtcctttgct ttttgttgta aaaaaaggat caaatatttt tgatagtatg tctttaggta
    19921 ttccaatacc cttatctgca attgcaatct taatatattt gccggcaggt agcagtggat
    19981 gctcattcag tgcaaatgtt atattatgtg caccaacagt aattgttcca cccattggca
    20041 tggcatgcac agcattgatg acaatatttt caatcacctg agctatctga ttataatcaa
    20101 attctgcatt ccataaatct tcatcacaat taaattctgc ttttacattt gaaccactta
    20161 gagcaaattt gactgtatcc tttaacaaag gaataaccga ttgagttttt ttaattggtg
    20221 taccaccttt ggcaaaggtt aataactgcc cggtaagtcc tttagccctt tggactgatt
    20281 tcaacgcctc ccctaataac tcagagatat tgttatcttt tatataggta gaagctaaac
    20341 caatataccc cattactcct tctaatatat tgttaaaatc atgggcaagg ccggcagcta
    20401 attgtcccag tgattctaaa cgctgtgtac gctgcacggc ttctataagc cgtatttttt
    20461 cgctcatatc tctaaatact aaaacaacac ccagtattct acccattgca tctttgattg
    20521 gcgcagcact atcggctata atgcgtcttg ttccatcttt cgctataagc atcgtatggt
    20581 ttgacagctc gtaaatctgt tgtgtttgca ataccttttc aacgggattg tcaagaggtt
    20641 tgctcgatag ttcatgaaca ataacaaaaa tttcagagag cggtttacct tgagcttctt
    20701 cctgtgaata acctgttaat tgctgcgctg tttcatttat aacagcaatc ctgccattag
    20761 tatctgttgc tataacacca tcaccaatgc tttgcaaagt aatccttaac ttttcctttt
    20821 cttctataag tgactcttca gccattttac gatcggtaat atcaagaata gaaatcatcc
    20881 ctgcaggacg ccccttgtag ataacactta ctccacttaa atacacccat tttatcctgc
    20941 catcctttgc tattattcta aactcatagc tatccacagt atctacattt gtttgtcttt
    21001 tagtaccccg ctccctcact atttgtttat catcaggatg cactatatcc caaaaattca
    21061 tatttaaaaa ttcctctgcg ctatatcctg caatatttac agcggcagga tttgcataaa
    21121 tccatttatt atcctgatat aaaagtattg cagtaggagt tgaatttgcc aaacttctga
    21181 acttttcctc actttctgca agtgcctgct cagattttat cttctcaatc ttttgccagg
    21241 cactgtccat taacatggta atctgcaata tatcaatctc agtataatct gtttctttat
    21301 tggcaacacc aactgttgca acaatcctgt tatctttaaa aacaggtata cctaaaaagc
    21361 gttgtatatc cacatgcccc tcaggatagc ccctcttggc tggatgataa tcgttgtaat
    21421 tattaataac aaccggacga gcctgcttaa ttgtttctac ccatatgccg gcaccttcta
    21481 cattatatat taccggcttg tcttgaatag cacattgatt cattaccaca tctgaccatg
    21541 ctttaataaa acatcttttt tcttcttcat tatattccat agcgtatcca attgtgctgt
    21601 ccgtcaattt aatagcttca ttgagaacat gataaaatag ttcttcctct gtttttgctt
    21661 ctttctgtaa cacagcaata atcgattcca atcgctcctt attctttaca agcagttcct
    21721 ctgccatctt acgctgtgtt aaatcatgtg caactataag aattgcaacc gttttaccat
    21781 tctttacaat tggagatgat gcaacctcaa cagggatttt tttctttaat ttattaataa
    21841 tttcagtctc atattgatga agtcccatat atccatttat gcgctcctga tgtaatgtaa
    21901 caatagattg atggtatttt ttaggaataa atttaagtat atttttacca actatctcgt
    21961 cataaccata gccaccaata tttatacctg tctggttaac atattgtatt atcccattca
    22021 tatcatgcaa aactataata tcgtgtgtag tttcagttat tgacctgtag cgttcctcgc
    22081 tctcttttaa cttttcatct gccaattttt ttgctgttat atcttcatag gtacccagta
    22141 cccctatgat ctcatcccca aggcgcaatg gtattttact ggttctaatc cacaagttgc
    22201 taccatcagg cgctgtttgt gactcctcat atccaatttt tggtatgccc gtttctatta
    22261 cctccttatc atccatgcgg tatagttctg cttcactgcg ccaccccata tcatagtccg
    22321 tttttcctat gatgtcctct gtcttttcaa atcctgcatc aacggcaaaa tgtttattac
    22381 atcccaaaaa tcgtaaattt ctatctttcc agaatacacg taccggaatt gtattaataa
    22441 cattattcaa taattgccgg gattctttta aagcctgttc tgtttcttta aaatgggtaa
    22501 tgtcggtatg cgtgccatat atcatcaacg gttgcctatc aggcgtccac tctatcacct
    22561 ctcccttcgt cagtatccac acccagtgtc cctgtttatg cttcatcctg atttcacatt
    22621 catagtatgg tttttcacct gtataatggt ttaccagatc ttcatatgct ttttttacat
    22681 catcagggtg tgttagcatc tcccaggttt ttatagaaat tggcgatagt tcctcaagag
    22741 tgtaccccag cagatgagcc cacatttcat taattataag ggctccagtt tgtagattcc
    22801 attgccatgt gccagtttga gtagcttcta taacattttc catcatcgtg ctgtattctg
    22861 caattttttc ttcagctttt tttctgtcgg tgatatctgt atacattgca aatgagccaa
    22921 tatattttcc tttttcatcc attattggct gccctgaaac tatacaccac agctcactgc
    22981 catcttttcg tttcctcctg tcctcatata catcagcaat tcctattact cttcttgttt
    23041 caatccgttc tttcaataca gggatatcat cctcaaacat aaaatcaaaa ataggcctgc
    23101 ctatcatctc cttttgggca tatcccagca tatccgccat cgctgcattg acgtaggtag
    23161 ttacattatt actatctatc ctccatatcc cttcttttgc agtttcaacc agtatacgat
    23221 attgtttttc actttctttt aattggttag taatgtcttt caactcagaa acatcacggg
    23281 aaacagccaa tattcctaaa ggctttagct gagcatcccg tatcattgat aaatttacct
    23341 ctaaccatct ggttgatcca tctttacaat aatgctctaa ctccataatt ctgtttcggt
    23401 cgggatctac attgccagcc ttttcttttt ccatctcttc tgcataaaca ttgaatgctg
    23461 actgcagtga atcgggcgtt aaaatctgat ctaattttcg ttttttatgc tcctggggcg
    23521 taaatcctaa aattttttca atggatgggc ttacaaaaat aatattaaaa tccatatcaa
    23581 aaatggtaaa aatatctgca cttttttgag ctatgatgtt gtagaaatct aattgttgta
    23641 aatcacgtcc tgaaacggtg gatgatattt gttttttctt tctattagat gttccatcgt
    23701 gttttgatgt tttttgcttt ttatggctat cactcatata tggctccaca tatgattaaa
    23761 atgtatcata attaactatt gtatggcaca ttgaaaaatc atgtttccat tattgaaaaa
    23821 aaataataga acttttgtca aatatttatg cccacaacaa aatatcagaa atataattat
    23881 taaaaccccg cttataataa taattgtaac tgttaattaa aacaactaaa ataatagatc
    23941 accatagtag ttgcgttaaa attttacaat ttactacaaa ttttagttga aacaaaatca
    24001 tggtatatct atatttgtgt atagtaatat ataatgcgca aaagcaatga ggaatattat
    24061 ggcaacagct ccatcatcca aattacctcc tcaattaaaa ggagagatta ttaatcttat
    24121 cgaggaacgt ttgaaagagc tttatgtaac atgttctgat ttcagagctt tacaaagagt
    24181 catgaaagag ctcgctgaag ctgaaaaacg cactgaacaa cgcatcgatt cattaacaca
    24241 gcgtatggaa gagctcgctg aagctcaaaa acgtactgaa cagcacatgg atgtattaac
    24301 acaggcatta acacaaactc agctagaatt aaaacagctt gttgaagaac ataaggttac
    24361 acgaaaactt ttgggtgatc ttaccaatac agtaggctac ggacttgaag ataccattat
    24421 gaaatatatt catgattatg ctttaaaaga atatggaatt gctattacca ctgtcgatag
    24481 acgatatgtt acctaccctg atgggcgcta tgacgagatt aacatctatg ctaaaggtac
    24541 caaaaatggc acaaatgtat atttaattgg tgaaagtaaa gctcaattag gttaaaaaag
    24601 atgcagataa ttttaataaa atgatttcac gattacatga ccagctacac ggtgaaatta
    24661 ttccattttt agtttgttat agctgtgctc cagatgttga acagtatatc agcactacct
    24721 acccaatgat tcgcattcat aaaagctttg agttttataa atataaaaaa agttttgatt
    24781 aattaacatt atatttcctt atcctcatcg ctctgaagat actgtaaaat ctttctgcgt
    24841 attgaatcag ggaggcgtat tcccacactc ttcaatctac tttcatattt tttaaatgaa
    24901 cgaacatgtt gtgaagttgt tacaaatatc ctgaaatatg atctaatgct cccttcaact
    24961 tcttttctaa attcgatgtc agcatttaac atatcctcaa gaggtgctat caccctgtaa
    25021 ttgatagggc ggcagataga acggtatgct acaaaacgtt gcaatgtttc tgattcataa
    25081 tttttatctt tgatatcctt ctccacctca tttaagttta tacaatcaag cacctcaaat
    25141 ttatattctt cctgtaatgc atttccaatt aacagtgcag catttttaca acaatcacag
    25201 gtcaataaat gatcaggaca ttcaaaatca cagcgcaaat agagcaccat atcaatatcc
    25261 gaactgggct gcgacaaacc aacattaact gagcccagta catccattgc aatatcctct
    25321 atggctggtt caactcgctt tactatttcc tccaattttt ccatacgcag ttctgattcc
    25381 tcagtctgga acatcctgaa aaaattttta atttctttaa acagctctat ctcaggtata
    25441 tgtgaatatt gctgcacatt catatcaaca ttcaataatt tcttttttgc actgaaacga
    25501 atatcatcaa tgacagtctc cacccatttc ccattttttt tagcatattc cgctaaatca
    25561 tacacacgtt cattatatat ctttctatag agcacatgag ctactgtata atcctgtaat
    25621 tcaatctgat aaaaaaaacc tccctcaagc acatcatgat ggactgttgt aatatcaccc
    25681 agcggtgatg gattaagata tagcgtattt tcaacctcct gcatacccca tgagccatga
    25741 atatgtccgg ttaaacaaag tattacagga ttgttatcac aatatgttct taatgccggt
    25801 gagccggtag gaccaacagg tgcaagccaa tctagcacac cgtgagcagg ctgatgagta
    25861 gcgatgatag tagggcgcac cgctttgaaa aaattataca tctcattttt gcgatcatca
    25921 attccaattc cagctttata gggtacaaca tatttttgtg gtataccggc agtaaaggta
    25981 tcagcacctc cataaccggc aataacagct tcatccacct gataccagtg tagatgcaaa
    26041 tccctgtcat gaagtgatgt atactttaaa tccatatcat aattacccgg aagggcaaat
    26101 atatggacct gaggtttaaa actaaaaata ttggcaagca ccttgtattt ttgctgcatg
    26161 acccttcgag cacgaatggt atactgctga tatttagctc ctaactccgc aacttcttca
    26221 gtaatatggg gcatatccat aagctcatca acaaaatctt ctactgtaat atcatcgcgc
    26281 cccatttttt ttcgcatggt attaaaataa agttgcagct cataataacg aatagcgcta
    26341 tccatattat aaaatgggat atcaaccaga tcacctgata taatatagac atcagcaatt
    26401 gtctcgtata acaattcacg aacgcgttca aaagcaccgt gaacatcagt acaataaatt
    26461 atccgcatca gctcaccatt cttttttcgg tataggttcg aaagacaatc atgactgata
    26521 gtattcccag aaatagaaat gcagtatata acaaactatc gccagcacgc ctgtatactg
    26581 tgacagtatt ggtactggta tcaatatccc caatacaata agttttttcc attaacggta
    26641 ttgaagctgt aatgcgcccg tatggatcaa tgagcgcagt aaaaccagaa ttgccagcac
    26701 ttacaaacca cacgccattt tctatagccc tgaaagttga aaagtaaaaa tgctgcatgg
    26761 gccccacagg attattggtc catagcaaat ttgttatatt gacataaaaa tcaatccccg
    26821 cttttgcata cttcctgcac agtgtatgaa aaatattttc ataacatatt aatggcgcac
    26881 attttaaccc attaactgat aagacaacca tcttttcacc aggcataaaa tttgaaccgc
    26941 caaatgcatc aattattgta ttaacaaacg ggaaaagttc accatacgga aaatactctc
    27001 caaaaggcac cagatacatt tttgcataag tatctacaac ttcgccatta ctatttataa
    27061 gcaccatgct gttttgcggg taataacgac ggttaacaaa atcttcaaca acgccaatct
    27121 caccggttaa aagtgctacc ctgtagcctg cagcaagcga tagcacctcc tctccaaaac
    27181 ggttaaggtt gccattataa tagccatacg aaatattttc aagtgttgcc gactcggacc
    27241 acacaataag attgggatta tgctgcattg cctgctgagt cagctgtttt aaaatatgca
    27301 gatacctgta cctattctca atccagtttt cccacggcga gaaacacgat tgtatcatgg
    27361 ctacacgcaa tgttgaattc tttgaaggtg gatggttatg caaccgtacc catccatagg
    27421 ctgttgcaaa aattactatc acacatatta tcgcacatgc ccataatggt tggcgatagt
    27481 tattgttcat tatccttgca gtgatatatt cacttatact actattggct gcaatgataa
    27541 taaaggttat gccgtatatt ccgactatcg atgcgatctg aataaagttt gtgaatggat
    27601 attgcgtata tccccagaaa ttccatggga aagccatagt tcctactgtc tgtacccaat
    27661 ctatagctat ccatactgat ggatagatga gcatctttgg tacatttgat tttcgcgata
    27721 gatattcagc aacaaatatc tttgtagcaa aaaatactga gatacacgga ataaccaatg
    27781 caacaatgat aggttttcca aacggaagcg cctgtccaaa atcacctatc cagtaataca
    27841 cagctccaaa acccagaatg ccagtgacaa atgaaagtat atacacctgt ttcagcggtt
    27901 tattaccgac aaaacaaaac agtggcacca atgataccca tgcaaaaact gaaagcaaag
    27961 gcagatgcac atttaatgat gtagtaaacg aacaatacat taataatact gacaacaaat
    28021 agtaattatc atacaaaaag atagccaatc gtttcactat gttcattgat gcttccctat
    28081 catcgtcata aattcatcct ctgaaagaat gcgtatttgt aatttttttg ctttatctaa
    28141 ttttgatcca gcctcttccc ctgcaacgac aaaatcggtt ttactgctta ccgaaccaga
    28201 tgcccttcct cccattgatt caattaaccc ctcagcttcc tctcgtgtca tattttgcat
    28261 ggtaccggta aatacaactg tttttccttt aaaagggttg tcagatgctt cctgaacctt
    28321 ttcatctttt acggtaacac catttgcaaa catctgacgt ataagtgtta atgaaccttc
    28381 attatgaaaa aattcatata cactctgcgc tacgcctgga ccaacctcgg gaattgtcat
    28441 aagctcttcc tgtgacatca ccataagctt ttgcaacgat cctgcatgct ttgctataac
    28501 ctttgccata tgatcgccca cattgcgaat gcccagtgca cgcaaaaaat gtgatagcgt
    28561 tacttccctt cttttattga tagaatcaat gatcttatca gctatctttt cacccattcg
    28621 ttctacctgc aataggtctt cacgttttaa tgcatagata tccgctattg ttttaatctt
    28681 tttttgatcg tacaaacgtt ttaaaagctc cggcccaaca tactcaatat ccataccgtc
    28741 ctttgaaaca aaaaagcaca atgtctcata ctcttttgcg ggacatgaag cattaacaca
    28801 acgaatataa atatcttcat gctgtaatgt tgaattacat gcaggacatg tttccggtac
    28861 cgctattgga cgcacatcct tccgtttatc cttaataact tctactacct tggggataac
    28921 atcgccagcg cgtattacct tgaccgtatc accaatcttt atatcaaggc gtttaacctc
    28981 ctgaaaatta tgcaaggttg cacgttttac cagtacacct ccaatattaa ttggctctaa
    29041 atttgctacc ggcgttatca atcctgtacg tccaacctga taatcaactg attttaaaac
    29101 tgttattgct tctcgtgcag gaaacttcca tgctattgcc cagcgtggcg ccttagaagt
    29161 gcttcccatt ttctctcgta ctgttatatc attaaccttt acaacaactc catcaatatc
    29221 aaagtccagt gtatacctgt tctccatcca gtatttataa aattgtttta taccctgcaa
    29281 caaaccaacc tcataataat gaggcgcagg cagtcccacc tttttaaaat aatcataaag
    29341 ctggctctgt gtattaatca tagatgcagg ttccattttc ccaattccat agagtactat
    29401 gtccaattca cgttgtgcgg taatattggg atctaactgt ctcagtgacc cggcagcggc
    29461 atttcgcgga tttgcaaact cctgctcacc attttgtctt cggatttcgt ttaacctttc
    29521 aaactcacca tgacgcataa tgacctcacc gcgaacactt aaataatcag gagcatctcc
    29581 cggcaacttt gtaggtacgc gtttaatcgt agcaatattt tctgttacat cttcacctac
    29641 ttcaccatcg ccacgtgttg aaccctgaac atagatgcca ttgctgtaca ccagttctac
    29701 agccagccca tcgtatttca actcggcaca atattcaata gtagatgtcc ccagcagttt
    29761 acagcagcgt tcataaaaat cattgatgtc cccttcgtcc atggcattgg caaggcttaa
    29821 cattggtgga tcatgacgca cctgctcaaa tgaggtctgt atagtagcac caaccttttt
    29881 tgagggagag tcatcgcgcg ttatttcagg ataatttttt tcaatcgtta gcagctcatt
    29941 cattaactca tcataggttg catcatcaac caatggttta tcaagcgtat agtagtagta
    30001 attatatttg tttatcaact ttataagttc atcataccgt ttttttatgt cttccataat
    30061 acagtactcc cgctacaata aaaattttaa ctttacacac tatgttcttc ccatacaata
    30121 aatactatca atacgtcaaa tatatcgtgt gtcaggtact tttaccaata gttacctgct
    30181 cctgctaata ccaaagtgct ctagttttaa tagacttgag caacaggata ctatgatata
    30241 gtcttgatac tactatcatg tttttttatt actaccatta taaatatact acaccatgat
    30301 ttttaaaata catgtgcctt tagcaaaact gtaacaaacc ttattattac atatccctta
    30361 taaaccgcaa tgttgtatgg cattgtcata aaaacaaggc aattgcatct acatatcctg
    30421 aaaaaatatt aattgactaa ataaacaatt ttctttcatc tatactataa atcgtagaaa
    30481 ccttacatag tgaggtaata aatgcaacta aaaaaagaga tcaaaaaaat taccataaag
    30541 gacccattcc agcagtttaa agaaacagaa cgtgtcaatg aaatgagata ctgtccgtta
    30601 acaggaaggg ttagccgtgt actgggacta tcgcttaaag aatttgcatc caacgataac
    30661 acccatatca ttgaagagtc aaagaaaaca tgtccattct gcccacaatg tattgatgct
    30721 ctgaccccaa agtttacccc ccctcttcaa gagcagtatt cgtggggcaa tgctcgttgc
    30781 tttcccaatg catttccata cgatgaaaac aatgctgtag taaccatcac cgatcagcat
    30841 tatgttgctc ttgataaatt tacgcacgat atgctctctg atgcatttca ctgtgccacc
    30901 aattttttgt ccgacattga ccctgtgcag tatccctatc agtcaatcaa ctgcaactac
    30961 atgccggctg ccggtagctc actggttcat cctcattttc agattattgc atcctcaaca
    31021 ccttccagct attataccga tatactatcg aacataacaa taaagcccaa tctttttcat
    31081 gattacatca ataccgaaaa agaacaaaat gaacggataa taggacttga gggcgatagt
    31141 tcctggttag tggcttttgc cccaatggga cagtttgatg ttattggaat actgcacaac
    31201 cacatccccc tgaaagattt ttacaggtat tgcaatactt ttattaccta tatactaaaa
    31261 attttgcagt atatggcatc taaaaaaatt gataatttca acatggcact gtatggtgtt
    31321 accaataatg ctgacttttt gccacacatt cgtatatgcc cgcgcactac atttccaccc
    31381 tatgcaacaa gcgagataaa ctacctgcag atgttacata atgactcatt tacattactt
    31441 aaacctgaga ttatagcagc cgagataaat gcttatacac catagtatta cataataatt
    31501 cttgacaaat gtatggaatt atatagcatt aagcaatatt tattcaatct cggtgattgg
    31561 tgctatccat gcgtattatt gcatgctacc atgaagtcat gcgtacgatt ctaaaagttg
    31621 attatatctg aatgttagct attatataat agctaaattt tttcataaac ggtattgcca
    31681 ttattttttt gattgtatat attttttgta gatacgatgg ctcagtggat tagtattatt
    31741 gagtaacagt gtgagaggga tgagacaata tatgcttcaa agtattttgt ataattatga
    31801 tcagaggata catttagaag agctcttaac gttttccatg gcattgctgc ttaatatatt
    31861 aattattata ttttctcatt ttacattagc acctgttacg tttacaatta atatagcaat
    31921 catgctgctc attatagcaa cagtatttgc acaacggttt gcaacttcaa agttattttc
    31981 catttacagg gactggtatg tattgccgct gttagtggca atcttcatgg aacacaatat
    32041 gcttgttcct ctcatcaatc caaatgatat cgatacctat ctcataaaaa ttgactattc
    32101 attatttttt ggcaatcatc caactctatt attagagagg gtgatgcatc cttttgtaac
    32161 tgagctttta caaatcgttt atgcaagttt ttattttctt cctttcacgc tgtgcctttt
    32221 actgtatctt aaagaaagaa aggttgaatt tcatattgta gcatcatcca ttatgtttgg
    32281 attttacctt tcatatattg ggtattatat attcccggct ataggcccac ggtttacctt
    32341 agcacaactg caaacaaaac ctctggaagg gttatttttg tttaattttg tacatcatgg
    32401 attggcatat ttagaaggtg taacgcgtga ttgtttcccc agtggtcata ctctagtttc
    32461 aatactaact gtgctattag caatgagata tcataaacga ttcagcatta ttgccacagt
    32521 ctggacagta ttgctgataa tctcaacggt ttatcttcgt tatcactatg tcattgatgt
    32581 aatagctggc ttttttgtag gcttgatagc atttcgatat ggccaaatgc ttgcacgtct
    32641 ttatatcttt ggtaataccg aaacaaaaac tcctttgctg gaaagtatcc ggaatttgtg
    32701 gtattggcta ctcgatattc gaagaaggta atagttgttt ataaaacaaa ataaccatat
    32761 atatataaca taatccctgt taataaaaat aatagggttt ttaataataa tgtttcttta
    32821 cctcgtacaa atatatcaac aaatgccatg atgattccaa atactgctga tgctataccc
    32881 attttcacaa aatccatact tacataatac acaacaccca ctattgtacc gccaactgca
    32941 atatcgacta tatcacgtat acgtacatac cgttttgttg gcagggcagt ttccttcttt
    33001 acaaaagatg gaacttcttc aggtttaaag tatcgttttt ttgttcttgc taattcttcc
    33061 tgtcttttct ttttacgggc agcaacctcc tcaggggttt cgccaatacc ataatgttta
    33121 ttgatcagac gtaatacttc tttatttggc agtgttgaaa gtatttcaga acgtttttcc
    33181 aatttttcct gcaatggtac cggtctgcct gatgtagcat tgcgttcagc agtaatacgt
    33241 cccttatcaa taactgcata gtaaggatta ccaccttctt cgctatcctc aagataaatc
    33301 ctaactcttt tatctttgaa gctataatca acacgtatat aatcgccatt ttccatatta
    33361 cagtaaaaaa cccgttgata acctcttatt tcggaagggc gtcttttcca gtatgttttc
    33421 tttatttctg gaagtattat tggttttgat gatatccttc ttctcattac agagccttta
    33481 tatattaaaa tagaaaagag attgactcat ttgtatgcca atctccttcc aacttacatt
    33541 atcgtaattt cttcttgtga cgattaagtc ttcttctttt ctttcgtttg tgatttgcaa
    33601 tttttcttcg ctttcgcttc ctaccacagg gcatattgtt tcactctcct tattatataa
    33661 aaatatatca ctgcagcatt atgatacaaa atctaactca ctatcaggat aaaaaacttc
    33721 aggaaaacca aaaagaatta ttgccgtttt taaaaaaact tatatatagc tacaattttt
    33781 gcaaaaataa aaattatcca gcaatcaata tactattttt taaagttatc gacatctttt
    33841 ttaacagacc cagaatcttc agtattgaag atatcaaatc ctgcttcttt atctgaggtc
    33901 actatcacct tcacattgtc agctaatttg tcaatataaa tatagcgcaa aatatcctta
    33961 tttttttgaa tcaattctga tattttctca agatgctcaa tacgttgttt gcttgctaaa
    34021 ccatcttcat taagtttttg ctgcaatctg gcaagctgca gagcattatc cttttcctgt
    34081 tgtaatacct ccagtaaaaa acgtttccct tctgcataga ttgcataatc cggaagataa
    34141 acattgccaa ctttaacatc atcaataacc aatcctcttt ttacacatac ctcttttaca
    34201 gtgttgatta caacagtaaa ttcattctga aaatttgcct gcaacggaaa atacttatat
    34261 acaggaacaa gatatttcct gaactgagta ttgacagcat caaccaatgc tatagtaata
    34321 atctctttta catattcagc attggaatgc atacttttta aatcaagttg aagcttatca
    34381 gcatcaaaag tatactggat tgatacgggt attgttattg aataattttt actttttatc
    34441 tgttcaagtg gcggtatggg gacaataata tcagctttac aactaccatg aagaggaata
    34501 taatttactc ttaccggatg aaataaagcc tgaggtacta cattcgcacc ttttttcaaa
    34561 agcccgataa tctgatcttc cttatcagtt acaattgcta caacatcatc tccaacaaaa
    34621 acaaccgcct ttacaaaaat ataaacagca gcagcaatta taaatgctgc aattaccaat
    34681 tttttgtact tactcatacg ctattcttca tctttatatt gcaccggttt tgatggcata
    34741 attgttgaaa cagcatgctt ataaatcaga ttttgtttct tgtcgacctc aaccaatata
    34801 gtaaagctgt caaagctaat gaccttccct ttgatgggta cgccgttcat aagataaata
    34861 gtgatttcta ccttatcctt tcgtgctgca ttaagaaaag tgtcctgtaa attttttgtt
    34921 tgtttgccca ttgtgcaacc tctttatttt taataaattt ttattaaatt ttttatattg
    34981 ttttgcaaac ttatagaatt acataccata tattaaaaca atatcaataa ttttttaatg
    35041 accactatat ccattcattg ataatttttt ttattccttt accatcattt gctggcatcc
    35101 ataaaggttt atatctctta aaccatgtaa tctgcctttt agcatagtgt tttgtttgca
    35161 gttttatgtc ttctattgca tcgctcaatg atatctcatt attcagaacc ctgtgcaact
    35221 gaatataccc tattgctttc tgtgaattta aatcttgagt gtatcccatt gctcgcaatt
    35281 tttctacttc ttctaccaat ccattgttaa tcatggtatc tactctgcac tcaatgcgct
    35341 tatatagtat atccctctct atatcaactg caattaaaag aatttcaaga tcgcttttca
    35401 tatgatcaga ttgcaaaaat tgcgataatg gtaatcctgt agctctgaat acttccaaac
    35461 ctcgtaaaat ccgttgtgta tcattgggat ggattttatc agcccaggct ttatcatggt
    35521 gcgatagttc cttataaaga gcctgtattc ccttaagttg caactcagca tgtaattgct
    35581 cttttatttt gctatcgatg cggggaagtt tggctaaacc ataaaaaaaa gtatgaatat
    35641 ataatcccgt tccacccaca aacaacggaa ttttaccatg ttgcaatatt gtttcaactg
    35701 cccctgacgc ttctctacag aaatcaccag cagtaaacca gtaatcagga ttgacaatat
    35761 caatacaata atgtgttatc aaagatcgat ggtacgggga aaccttccct gtaccaacat
    35821 ccatatattt atatacctga accgaatcta ccgaaactat ctcaaatagg ttgttagcta
    35881 tcgaaagaga tatatctgat ttacccgatg ctgttggccc aacaagaata actgctttct
    35941 ttttgttaac ctttttcact cttataaaaa attattattt caaatattca atcgcttaac
    36001 tgttccttga attcatattt aatgattcca ttcagcatga gatgcaatat atgcggtaca
    36061 actttccagt ttttgtcagt ttcaggatat ccttcaatat tacctaattt atccactact
    36121 ttcattgcag cacatgaaaa gatatattta ttatcttcca gcgctataat tttatctaat
    36181 ggtataatca tgtacaccct ccagcaaatt tttaactgct ttttccttaa attcccgcct
    36241 gagaatgatt gcctttaaaa tagcaccaag atcttctaag gctttctgta aatcatcatt
    36301 tacaacaata taatcataat tgatatattc acgcaattca ctcacagcat ttgtcaatct
    36361 tattttaatt tgttgctgtg tttccgttga tcggtgacgc aaacgttttt caagttcttt
    36421 aattgacggt ggaataataa atattgaaac aaccttttgt aacttattac gtaaagcacg
    36481 tgccccctga acgtctacat caaaaatagg tatttttgaa ttagcattta ttctgtcaat
    36541 ctcttttttg gttgtaccat aataattatc atgaaccttt gcccactcca caaactcgtt
    36601 attatccacc attttcataa attcatcata acttacatag taataatctt tcccctcttt
    36661 ttcatttgac cgtatgggcc gtgttgtagt tgaaatagca aactcataaa ttggattctg
    36721 tataataagt ttctcaataa gtgttgtttt ccctgcacca gaaggtgctg aaataattat
    36781 tgctatcatc atccttcttc gctttgattt aatctatttg ctattgtttc aggttgcatg
    36841 gctgacagta tcacatgatt actatccatt ataattatag cacgcgtttt cctgccatgt
    36901 gttgcatcaa taagcatact gttttctttt gcctcttcac gcaatcgttt tcctgaagct
    36961 gatacaggag taataacagc aataacacgt tcagcaacaa cagcattacc aaatcctata
    37021 ttaattaatg ttgttgattt cattattcaa ttccttttaa actatgttcc tgcattgttc
    37081 ccgtatttta tctatatgat ttttcacttc aacaacttta tgggcaataa cagtattatt
    37141 tgatttagaa cctattgtat taacctctct aaacatctcc tgtgccagga agtctaattg
    37201 tctgccaagc tgtccttcac cagccattat ttctttaaac tttactatat gatcatacat
    37261 gcgtgttagt tcttcattaa tatcttgctt atctgctaaa atcgcaattt ctgtatacaa
    37321 tctgttttca tctattttac tatgtaataa tgattctatt gattttttta atctgctaaa
    37381 ttgatactca gcaacagcat gggcttgttt acgaatatct atacatgcat gttcaattat
    37441 ttttaaagaa gattcaagat cttttttaac agatttccct tcttttttgc gcattttaat
    37501 agtggcatta attgattttt gtaatgcgtt atatatgtca tcctttgatt tttttgaaag
    37561 aatcgaacgc tctttatgaa caacaccatc aagttgcaat aaagcatcaa cagaaaaact
    37621 tgctgcaatt tttaaacttg ataatgcttt ttgtacttca tgataatatt tcaacaatat
    37681 ctgtttatta atatggacag gtcttgattc tacccaatcg taaatgtcaa tgttaaattc
    37741 aacttttcca cgttcaaaac ttttttttaa aatagcattg atggtatcat cctcatattt
    37801 taatatcttg ggtatatttg tataaacctc cagatattta gaatttaatg tttttacttc
    37861 aattgagaat gtaaactgat ccgtactttg ttctataaat gcataccctg tcatactttc
    37921 catactataa tctcgctaca tctctttatc ttcacgtcct aatgacttaa ttttcaattt
    37981 accactttca agaaaaaaat ctaccgttct accataatct cccaatacat cctcggcaat
    38041 aatattgata tgatatttat ccaatacttg tttaaccttc aggacattat ttatacctat
    38101 agagccgata aatgaatttt ctggcatttt aaacattgaa gcaccaccaa ctattttagc
    38161 aacaatcaat tcaggatttg cccccttttt taccatttca tcaatcataa gagctatagc
    38221 actatcggca tattttttag gattcgttga cttatcgttg ttttcaggca acataatatg
    38281 tgccaatcca ccaactttgt tttccagatc ataaaggcat atgcctacac atgaacccaa
    38341 aattgttcgt aatatatccg gtgaatgaga taccttataa tcagctatcg aaactgtaat
    38401 cagttgaaat tccattatac tttctccttt ggaacatata caggtgaatc cattatcttc
    38461 acatactgga aatcatcgga cagagcgtgc aaggtttctg aatggccaat aaataaatat
    38521 ccccatggat tcaaaatatt ataaaaatta gcgatcagtt ctttttgacg agaacgatca
    38581 aaataaatca taacatttcg acagaatata atatcaacat tttctggata agaattttta
    38641 aaaagattaa gatatgaaaa tgcaacaagc ttttttaact catccttaac ttcatacact
    38701 tcaggattat caggcatttg tgtaaagtat ttttttaaaa tttcaggagg aactttctct
    38761 attcgcctgc ctgtatactt tccaatacgt gcaaattcaa gaactgatgg tgcaatatca
    38821 gtagctaaga tcgaaacatt ccaatctgat aaaagtaatc gtgattctaa aacacaaatc
    38881 gcaatagtat aaggttcttc acctgttgaa cagcctgcac tccatatttt cagttttttt
    38941 tcattcttca ttttaacaat tttggggata caatgatcct tcaatgcgtc aaattgccgt
    39001 tcatttctga aaaaatacgt ttcatttgtt gaaacaacat caagcaactc ttcaatctcc
    39061 ttgcttttat ctttaagagc gttaatataa ttaaaataat ctgaaaactc agaaaaattt
    39121 aatgccctga gtcttttacg caggcggtta gatagaaggg taatttttga ttcttttaaa
    39181 aagattccac tttcatcata tattaatttt gcaaacttat aaaattcatc ctcggttaag
    39241 cgtttgatat ttgtgaagtc aatcatattc caccatagaa aagtagcaat tcagcaatca
    39301 ttgtctcgtt gaaagattca aaataagtga tatttcgctt attgggattc ctgatatctt
    39361 tgatagtatc tgtaatgtat atccctggct gtataatttt gctatgaaca gatatttatc
    39421 atcagcattt acatacatct gctgtaaaac agtttcatct acatttaagg tgtcttcttc
    39481 aggggttttt atcattgatt cctcaggttt ataactatca ctttgaaatg atgttgtcga
    39541 catatcctgc atttcattat aatgcacatc aggtaaatct tttattgcaa cattcttttt
    39601 atacttgtat ttaatatttt cctttattct ggcaacaata acccggctaa gttccaacaa
    39661 ttgctctttc actgtgggga tttcgttatt ctcttttata atagcagatt caatagtgtt
    39721 ttgtttatgt atactatttc cattatcaaa atcattctgc attttgttca caacattatg
    39781 cggatttatt atttctgcaa caatagtatc atgtttattc ctactatctt tattattttc
    39841 attcagatga ttttttctat tatacgatct tgttttgctt ttttcggcag gcactggttg
    39901 cccattttca tcatctattg tgatatctat tccattaagc gcatctcctc tttttagcaa
    39961 ttcgcgtaat atgtttattt tatattccag taatgataaa ttacgttcag ccgtattatt
    40021 aaattcattt ataagttctt ccatctcttt tcgtagccgt ttcgatctaa attcagtagc
    40081 agaacgttct agttttaatg aaatgattaa ataaaatact gaagctatga gtatattaac
    40141 cattatgagt ataataagct gcatcagtta tccttcatta tccttatttt ttaacatcaa
    40201 taattttccc aattgcttcc tcttcaaaga tttcttgatt tccctttttg tcgtttttac
    40261 taccttcttt ttgttgtttt ccctttttaa attgtttttc ctcattaagt attatggtac
    40321 gttcagcttt tttattttcc tcaacttttt ctttttgtat attagcttta tcctgagatt
    40381 ccttactcaa tatatgctgc tgctcaacag ccgcaacatt gcgggaatgc tcaccacgag
    40441 caacttctat catctgtgca atattagttt gaagatcaat tggttttata accattggat
    40501 tctcctgaat aaaatttttt gtctatcgtc tcctccgtgc tattagcttt tgtgcttcat
    40561 ctaaatccgg tggttcatat tcagaaagcc ttatatcgga accctcaatg gaaaacttaa
    40621 cataactata tgggtctttt acattaaaac gtttgtcctt aatatatatc tcaacaccag
    40681 gaaaaattgt tttttctaca cacactttac ctttttgctc taacatagca agataagctt
    40741 tcaattcttc caatgataga ttaatttcat taagacgttg ctccatcttt tgcttttgtg
    40801 ctactaactt ccctaaaagt tcctccctgt ctttaggtag atgccctcct gcattattct
    40861 tttgaactgt aagggttgta acatctttcc tgactctgtc aaattcttcg ttaatacttc
    40921 ttttgttttc ctcaagttca ttaagctgtt gcaatacctt tggattcatc cctacccgta
    40981 ctgttgtttt tgtaaatgag tccgaaccaa tatattttga attgacttcc tcgcctgctc
    41041 gtataagtcc tccaacaata cgtgcccttc ttccattgca aataattcta ttacctgcat
    41101 caacatttga atgaactatt ccttcagcca ctattacatc tttctcagcc agtacatttg
    41161 cagtttgaat aaatttagca ataactgacc cgcctgtcga ttcaatcttt gctccctccc
    41221 ttcccattat cccctgtcgg attataacat cgccttcggc ttctaaatta gctttctgta
    41281 ctgtcccttt tacttcaata tttcctgcgg cctttacaac aaaattatcc tgtacgctcc
    41341 cactaacaat aaccgaacct aaaaatacaa tattccctgt ttcagtgctg acatcccctt
    41401 ttactacata aaccggctcc acgctgagct taccattttg gtatacaacc tgtccgttta
    41461 tttccgctgt taattctgtt ccatcctgtg aaagaattgt tcccttccca tattttatct
    41521 gaatatcttt accggaacgt gcaggtataa ttctattggt tattgttctt cctggcactc
    41581 cttcttcagc aggtaccttg acagccagca cctgacctac aacaacattt tcaagaagat
    41641 ccagatcttt aaaatcgaca cgtccctttt catcctcttc taattgaatg ccacttttat
    41701 caattcgtac tttgtaatca atatatgcat ctttgccatg tcttggaggc gtaccttctg
    41761 ccgctaaaag aggttggcta taatccattc tatccagata ttcagcaatc ctttgttctt
    41821 taattccagt aacgataccc gatcgtttta atgcttcaat aacatcatca acatccatat
    41881 gtctgccgca aaatcgtggg ggaacaaaat gaatatatgc cttcatctca tcatcagtaa
    41941 tttcaaccgt catagaacca tcatattcag gattaggtat ccattcacca attttcactg
    42001 gcttgccaga aggatgtttg atttctttct ctatttttgc catatcagca ttttgaattc
    42061 taagagaata aatcctattt gatacatcgg acaaatcaac acgtctcccc ccacctttcg
    42121 gcggagttac tgttaaccag atgccagttt tggttattct aattttggct gagccatcaa
    42181 catttcttgc tacatcctga actttcaaat cagcttcagg tacagcatgc agtttttgtt
    42241 caatcgctgt taaatcttct tcctcttcta cttttatagg tctaatcaat actttatatg
    42301 gttgtctgcc gattcctaat agacctgatg ttcccttctc caaaatctga taatcaagct
    42361 cagatatatc aacacccaga tctctggatg ccaattcaag agcctgtttc accgaatcag
    42421 catatacttc cacctcattt tcggctatat catcatcttt taatgttttt aacaattctt
    42481 ttagattctt cattatacaa aaaccagata tacattttaa ttataataat acctgatcag
    42541 attattcatc caacaatatt tgatttaatc cttcccagcc tgccacgcaa tcgcattatc
    42601 gccttagtat gtaattgtga taccctgctt tcagttactt caagaacttc accaatttct
    42661 tttaaggtta aatcctcgta ataatataga acaataactt tcttttcttt atcaggtaat
    42721 ttttttattg cctcaacaat atagtcgcgt atttcttctt tctcaatcat aacgtcggga
    42781 tttaatgtat caggtgcttc aagtgtttcc attatcgaca gttcatcatt ttcatcccca
    42841 acataccata tatcattaag cgacaacata gctgtcccgc tcaacttgtt cagtaaggat
    42901 tgaaattctt caatggtaac acctaactct ttggctattt cttcatcctc aacagttctt
    42961 cccaatttat tttccaattc agcgatagcc tgctcaatct gttttgcttt ttgtctgatt
    43021 gatcgtggta tccaatcaat tgaacgtaat tcgtcaaaaa tagcccctcg gattcgtgtc
    43081 atcgcatatg ttttaaattt tatcccgcga tcaggatcaa attttgaaat tgcatctata
    43141 agaccaaata caccatagga aacaagatca tcaaattcta ttgagcttgg cattcccata
    43201 gcaattttac cggctacata tttaaccaat ggtgcatatt ttattacaaa atagtcccgt
    43261 atatcctgat tttgtgtctt tgcataccgt ttccagagtt catcttcatc tgtagcatca
    43321 aatccttgaa attttttatt tgccatacaa cctcgttatt ttatttagtc cttatcacgc
    43381 ttgatcatag ttcgtatagc ttctgccatt atttttggtt catagtgaat ctttttgtca
    43441 tccagaacga tgtgcttccc catcttttta ggtttacttt cttcagttgt aaaagtagta
    43501 aaatccttag cttccaatgg tgtaaatgct tcgttatagg tagcccccac atcaatagac
    43561 tcattgccag aatcagaaga aacaatgccc tcccctttat cagaatcatc actgttttta
    43621 tactcagatt gcatcgtcac atcagatgac tctgtagcca tatctaatga ttgaccaata
    43681 ttcaatactt tgtttaatat ttcatacact tccggaacaa aatgcttgat cacaagcatg
    43741 gaagcataac caattgttgc atagacaatt gtaaaaaaca atactctggt caataatata
    43801 acaattctgt tcccaacagc aagcccaaag aataaagata ctataaatgc aaggaatcca
    43861 aatatcagag atactttagc ttccggtgaa atttctgcaa ttttcttaat attcaacatg
    43921 atttatgacc attttagata acactatcac aaatttacaa aatttacaac ctaacataaa
    43981 aaatagtatt aaatacatct taaatcaagt aataaataca caatgtatca atttaattct
    44041 atacaacacc atcatcatta tccatttcat aatgaccaaa aaatcttctg aaaaagctta
    44101 gcataccagt tcctttatca atgtctgact caatatttcc aatcctatct gcaataatct
    44161 ctacacatgt agaagccttt gatttaggat agctcactat aaatggtttt tgattgcgta
    44221 tggattttgc aacataaaga tcatcaaaga taaaacctaa attatcaact ttaatgttca
    44281 aaaactgccc ggcaatgtta atgactcgct gagcaacttt tttagcttcg gtaactgtct
    44341 gtgccctatt aacaagcaac tttactgtct tatccggatc gttggcagca atagctttaa
    44401 tagtaccata ggcatctgtt attgatgttg gttccggtgt agtaatgact atcacttcat
    44461 cagaagctac taaaaaactc aatacatttt gtgaaatccc tgcaccagta tcaatgatca
    44521 tataatcatc agcatttagt tctgccagcg actttataaa atattcccgt tgcttcacat
    44581 caagatttgc taattgatga aatcctgaag ctccagcaat aattttaatg ccttcaggaa
    44641 catcaataat tatttcctct aacttttttt tccctttaat gacatggtac agattgtatt
    44701 ttgggataat tcctaataca acatttacat ttgccaatcc caaatcagca tccagcaatg
    44761 taatagaata attacgttta gccaatgcaa ttgccaggct aacagctata ctggttttgc
    44821 caacaccacc tttgccgctg gttatcgcta tagttttggt tctgcgcgca gtattatttt
    44881 caactaccaa tcgccttaaa tttgcagcct gatccaagat taacctcctt aacgcatagt
    44941 tgattccagt atcatattta ccataatttc cggatctgcg acggcaatat catttggcac
    45001 ttcctgacca ttagtaaaat acgatattgg cttattatat ttatcggcta ttgagataac
    45061 attcccaata tatgaggtct catccacctt ggttactatg atactatcaa tatgcaggat
    45121 gtcatatgct ttaaatacct cattcaaatc actgcgtttg gtatttgcac taacacaaag
    45181 aatcttatgt atctcacaat tacatgcatc agcaaaattt tttatttctg ctatcttcaa
    45241 atcatttcta tggctcctgc ctgatgtatc caccattata atctctgctt tttcattttc
    45301 aactatttct ttaaattcat gagaatcagt caccacatga attggaatac gcattatttc
    45361 agcatatttt ttaagctgct ctgtagctgc tattctataa ttgtcgatag ttacaaacga
    45421 tactttaagt ccttgcctaa gagataaaat agcacctaat ttagccatgg ttgtagtttt
    45481 cccaacccct gttggtccaa caaacattaa aaccttttta cgtttgcctt tatcgatagg
    45541 tccacttaca atgattttac ttttaatcaa ttcacgcagt gttttctcaa ttttatacct
    45601 atccttcata tcttcagagc ttacattacg ttttacatct tcaagcaatc tattgcactc
    45661 atcatcatca atatcatttt ttcgtagaat ctctttaaac ggcttaaaat aaatatcatt
    45721 gctatctata acaatatcat ctataggttt tttctgtcct gatgccacca tatttttcaa
    45781 caacattttt aactcctgga actctttttc taatttaata tatggttgat cagtcctttc
    45841 caccatcacc tcatctaccg gtgatttaac aggatgtggt gattcagtag tttttatatt
    45901 tggtttttcc atgccctgaa ctattagctg ctccaacccg gtgtttggtt tttgttgagg
    45961 ctgactttct ggtttatcta taacttgctt taataaatca ttaatatttt cattgccacc
    46021 aaccgttaaa tcaatatttt tcctgggttt taattttgag ggttttacat caggtatggc
    46081 agcagttaac tcaacaaaat cacgcgcaaa taaacccgtg ttgaatattc caccttcttt
    46141 tatattttta tgactaatgg gaattgcatc atccccatgt tccattttca atttcatcat
    46201 agcttcatta taggttctgg cttttatctt tacatatctc attgttgtat acctctcttt
    46261 ataaatgtat taattttatg ctgcccgtaa taatgctgtt cctattacat ttactcttat
    46321 ttcaggaaca atctcattat acgataacac cacagggtcc ggaacttcac gctctaacaa
    46381 ggtaaacaat acactgcgta tgtgcggtga caccagaaaa acaggtgcat accctgcact
    46441 ccttgctctg gaatacgaat caaataaact atccagaacc tccctgtgag tatcaggatc
    46501 aagacttata attcttcctt ctacagggtc ttcgtgaatt gatgaacgaa taattgtttc
    46561 aatctcagga tcaactgtaa taacagataa cgtgttgttt ttatctgcat attccctgca
    46621 taactgtcgt gctaaagcga ccctgacata ttcagttaac aatccaatat ctttggtata
    46681 gtcagaataa cttgccagcg tttcaagtat tgtaaccata ttacgtattg aaacacgttc
    46741 acgtaaaaga ttttgtaaaa ccttttgtat ttctccaata ctcaattttt tttcaccaat
    46801 taactcattt accactgcag gatagtcatt tttaattgta tcaataagtt gctgaacttc
    46861 ctgccgtccc aatatttcat ctgcatgtct tcgtattatt tcggttaagt gtgttgcaat
    46921 gatagtggga cagtcaacca ctgtatagcc acgacgttca gctacatccc tgttatcagg
    46981 atcaatccat attgctctga gattaaatac aggttcagta aattctactc cttctatctt
    47041 ctcttcaaca gtgccaggat ccattgccat gagtttacca acctgtaaac taccatgccc
    47101 aacttctaca cctttgatta atatggaata atcctgcggc ggcaattcca tgttatcacg
    47161 tattctaatt ggaggtacta tcaatcccat ctcaagggca ctgcgcctgc gtatattggt
    47221 aattctatca agtagtgtcc caccctgatc aggatcagct aatggtatca ggctataccc
    47281 aatctccacc tcaagcgcat caacctgaat taatgggata actgattctg gtttatgtgt
    47341 ttttaatgct gcttcctttt gctcaatttt ggttttttgc atcttttctt cttcagtttg
    47401 tttcatgaga tagcctaaag cacctaaacc tgcagcaagc aaaataagtg taatctttgg
    47461 aaatcccgga ataattgctg acaatcccag agcagcagca gttatcatca atgcacgggg
    47521 ctgtgaacct aattgtgttg atagatcttt gcccaggctt tcctcagaaa ctgcacgtgt
    47581 tacaatgaca cctgtagctg ttgtaaccat gagtgaagga atctgagcaa ccagaccatc
    47641 acctattgat aacaaagtat aagtatgaat agctacatta aaactttcac cacgcattac
    47701 catcccaata ataaagccgc caataatatt tataatggtt attaaaattc ctactttaac
    47761 atcaccctga acaaactttg aagcaccatc catagctcca taaaaatcag cctctttcct
    47821 tacctcacgt ctacgatgca atgcctcttc ttcatttatt ataccatttg acaaatcaga
    47881 atcaatactc attaacttgg ctggcattgc atcaagtgta aacctggctg ctacttctgc
    47941 tgttcgtgtt gcacctttgg tgataacaat aaactgaact gccgttaaga ttatgaacac
    48001 aataaatcca atgacatagt tgccccctac aacaaaatcg ccaaatgtac ggataatctt
    48061 accgtcaaat gctgctccct gtaaaagtat taatcgcgtt gaagaaacat taagcgccag
    48121 tcggtaaact gtagttatga ggagcagagc tggaaatact gaaaaatcaa aagaacgagg
    48181 aacatacata acaaccaata gtataagtat tcccagcata aggctgattg ccaggagtaa
    48241 atcaagaaga aaggttggca gaggaatcac cagcatggtt acaaccgcaa taacgccaat
    48301 tcctaaaagc atatcgcttt tttgcatcca ggagaaatca tatattcttt ctgccataaa
    48361 ctacctcaac tttacatcgc aactttatag ttacgttttt tataaacttc agcataaatt
    48421 aaggaaactg cataaaataa atcttcaggg attatatcac ccacatcaac ccttttatac
    48481 aattcctgcg ccaggggcct gttctcaata attaaaatac tattgtcgag agctatctgg
    48541 cgtattgtta gcgccatagt atcaacccct tttgcaatta ccataggcgc atacattgct
    48601 tcaggttcat accgcatagc tatagcaaag tgtgttgggt tggtaaccac aacatcagct
    48661 ttgggaacct cccggatcat attgcgcata ataagctcgc gctgcatctc ctgcaagcga
    48721 gctcgtacat ggggatcacc aacagtttct ttccactctt cttttaattc ctgtttagtc
    48781 atcttcaacg attcaatgta ttcccgtttc tgaaaaacat aatcaggaat tgatagcacc
    48841 agtatacaaa ctgctgccca tataataatc tttaaagcaa taatagatac attctgaaat
    48901 gccaatccaa cgctaatatc cggtgttttt attaattctt caaaatcatt gatgattacc
    48961 agataacata taaatccaat tacaactacc ttgaaaattg atttaaaaag attcatggca
    49021 atctgtcgtg aaaaaaatat cttacgtgcc atggtagcag gatcaaattt tatctttgaa
    49081 agatcaaatt ttaatggatg agttgaaatt tggaatccta cctgtgcaat attaccaatt
    49141 aatgctgcaa tgaccgttgc tacaaaaatg ggaattaata tcttaaatga ttcaacaccg
    49201 gaggcaatag cctcatgaaa aaggctccgc tcagtaagat taaaatgtga aaagtttgtg
    49261 agatagtaat gcatcatcct gataattgtc tcaaaaataa atccacaaaa aacaacaatg
    49321 gttatcattg caaaaataat aacaagcgcc tgaggaagtt cctcagtttt cgcaacctga
    49381 cctttttccc gtgcttcccg taattttttt tctgttggct cctcagttct accttcatcc
    49441 tcagcagcaa aaagctgcat atcaaaaact atagccctgc tttcaaaaat cacgatttca
    49501 gcatctttat ctgtaatagc ataccatttc atactggcca gtaccgtatt accttaacaa
    49561 taaattgaaa tgttctttcc agtgaaacat tcataacacg aactattaga ggcgaaataa
    49621 ggatcatcat gccaaaggca actgcaattt taaagggaaa tcctaacatc attatattca
    49681 tctgtggtgc agcctttgcc agtattccca tactcactga cattaaaaac accactgcaa
    49741 caacaggcag tgaaattttt aacgctatta caaacatacc actgaatgaa tgaaccagaa
    49801 attttaaaaa tacctgaaca ctatctccct ttaaaccaaa agcaggagcc aattcatagg
    49861 aattatagat agcttttatc ataaaatgat gcccattgat tgttaaaaaa accagtaagc
    49921 caataaaatt tttcaactgt ccaataagcg gtattgaaac ctgtgccaat ggatcaatga
    49981 cttcattgat accaaagcca atctgcactg caaaatattg ggctgaaagc tgaaaagccg
    50041 aaaaaaccac gcttaccaaa aaaccaatat ataagccaat gatgatctcc tgcactacca
    50101 taagatagta tacacccata ttacccgtaa cagcaccagc actaattgat aaaagcggaa
    50161 atattaccag cgatacaaag aaggaaagcg cacctcgcat taataaagga ataactccac
    50221 ttgaaaaaaa tggagcgatc atcatcattg ctgtcatacg caccagcacc agaaaaaaac
    50281 tttggaactg ataaacaaaa tattccatta tcctccaccc agtttggaga tcatcatgaa
    50341 caattctttt gtatacgaca tcaatgtatg taacatccat gcagcaaata atataaacgc
    50401 agcaaaaatt gcgataattt ttggtacaaa cgtaagtgtc tgttcctgaa tagatgtagt
    50461 agtctgaaat attgatatta tcagtccaac aaacatacct attccaagga ttggtgctga
    50521 cacaatcatc gtaaccatga gagcttcacg cagcaatttt atcacttcta tatctgtcat
    50581 gttcttctcc tacataaatg atcgaaccag ttcataggtc agcaaattcc acccatctac
    50641 caatacaaac agcacaatct taaacggcag agaaatcata attggcggta acataatcat
    50701 acccatcgcc atcaaggcgc ttgctacaat catatcgata acaataaacg gaataaatat
    50761 atatacaccc atttcaaagg cgcgttttaa ttcactgatc ataaaagctg gaataagaca
    50821 atacgtagga acatcatcct gtgtttttgg tcgttccaat tttgcaagtt caataaataa
    50881 ggctatatcc ttcttccgcg tttgcttcaa cataaactca cgtaatggct gcataccttt
    50941 attgtaaaac tcttcattag atatctgccc tttcaaatat ggctgcagag ctttatcatt
    51001 aatctctgtt aaggtaggcg acataataaa aatagtcaga aaaatagcaa gtccaacaat
    51061 gacctgagta ggcggcattt gctgcaaaga aagagcacgt cgtacaaaat caaggacaat
    51121 aacaactctg gtaaaggatg tcatcatcat tatgatagac ggagctaagg tcaaaatagt
    51181 aagcagaaac agtatctgta aacttagcga aacatccttt ggtgttttag cctcttcaat
    51241 atcaaatcca atcttaggaa taggcatacg gacattaccc gctttctggg caaatgcagc
    51301 atcggatact atcatgccaa tacccataac cacaacaatc agtaaaatta ttataaaaaa
    51361 ccttttgtat ttacaacttt tcatataata ctacacacta tcgtccccat taagtttttt
    51421 taatcgctgt ttatatgtca gcaaaacatt atcattgcct gtatgctcct gattataaga
    51481 ataatgctgc gatatcccct cttttggatt ttttatcctg tcaaggacct tccctaattg
    51541 tgttgaaaga taatcctgaa atccacctgg ttttactgga gttgactttg aactgagcaa
    51601 gcgtattcta tcgacgtcat ctttatcttt tatctcagtt ataagattta tattattatc
    51661 agatacaccc agtacataca ccttccctgc tatatccacc acctgcaaaa acttattttg
    51721 acctaacggt acaactgaca aaatagtaat aacctcctga ccaacagcag caatcccaac
    51781 cttttcagta ataaatttaa aaaagaaata aaatcctcct atgagtatcc ctatcacaaa
    51841 tattgtttta aagattagcc atacatagga ttcttcctct atctgcggtc gttcgtaatt
    51901 atacagataa tcattttttg aagcctcatt ttctttttgt tcatcgtctg attttgtttc
    51961 aacaccaatt ggttcttctt ttttttcagt tatcgtattt ttactttcaa tcttatctaa
    52021 ttttgctgtt ttagtattct tagcatataa aaaattttca ttgggtcgat agttactggt
    52081 aatgggacat aatataaaaa tgagacataa taaaataaca ccatataaaa atttcattga
    52141 cgatacacat aaacgttttt ttatagttgc agcaacaatc attaattttt tttcctttaa
    52201 aactttttaa tgataattat gtttgcatct tagcaaatcg ttctgccggg cttacaatat
    52261 cagttacacg aacaccaaaa ttttcatcaa taacaaccac ctcaccacgg gcaatgagct
    52321 taccattaac aagtaaatct actggttcac ctgccagttt atccagttca ataatggaac
    52381 cttcgcccaa ccccaatata tccttaacaa gctgacgagt acgacctaac tcaacagtga
    52441 gcgtcatcgg aacatctaac aacaaattaa tattccctgt aggtaccgac tgcatacctt
    52501 cacctaatgg agggaatcgc acggggctaa tccctacttg agcactcttc tgcggtgcag
    52561 cttcagttcg gtactcctgc tgttgcatct gaggaacata cgaaccacgg gcaatctcgc
    52621 cagcaagttc aatattcatt aaatgataca tctttgagtt tataagattt tgaatcgtca
    52681 ggttatatgt tattttcaca acatcaggag ttcccggtac agctatatcg ccctgaacta
    52741 tcgctaaatc gggagctgtg gtgttaacac ctttaccaat cttattgctc aactgtgtag
    52801 caatagtgga catcatctgg ctaacaaatt cctgcaacgt gctttgatga gcttcactta
    52861 atgtagccgg aggaataccg ctatcatctc ccatcatgag tgatgaaatc acacctgcat
    52921 cgttcattga taacaatact atatttcggc catttacccc acccgaaaaa tttactgaaa
    52981 cctgaacgta ttgtttggga aaatcagcac gaatctgatc ggcatttttt acttcaacca
    53041 gcgcattgcc aattattata tttttaccca gatagcctga cagtgcaggc attacactgc
    53101 tcatgctgct gtttaaaaaa tctgcaaccg cctcacgttc aaccggagaa agcgctgcct
    53161 gtacaggagc aaaatcacct gctggtaccc ttgctgaacc ggagagcata tcatcagcac
    53221 cctgtaacag ggcatctatt tcatcttgcg acaaagaacc gtcacccatt gtttcctcca
    53281 atgtgtttga ttcgtatatt catgtaatgc ccatctactc ttttcatcat atgttaataa
    53341 gatacacaaa agtaaactaa tttacaacaa attctctaaa ccatacttcc ttaatcttgc
    53401 cttttaaaag caatacattg atatgcgctt ttatctcctc tgacaagttt accgtatcct
    53461 caacagaatt caactcttca tacgatttat cacgtaacag tatattgata atatgctgta
    53521 tctgcggagt acgctgcacc agctctgcat tcaattcaac attctcttca tatcccaacg
    53581 acactgttaa ctttgcaaaa tgcggttcag gatcattggt tgtcactgaa aaagatggaa
    53641 tatcaaaatg agctaacggt gacggcggag gtgccaatac gatatcctgg tcccgctggt
    53701 atcgttgttc ctgaacatat tttgcaacta aatatgatat cccaattata ataaaaaatg
    53761 ataatatgac tcccgcaaca taaagaagta tttttattat ccgtgaagtc tcaaagacac
    53821 ggcccgcctt cgcttcttca ggtttttcct gctcttctac ttcttcaaca ttgacgcgtt
    53881 cttcatcacc catgtactta cctcaccaac aatttttcat tattcagaag tattatatca
    53941 ataaaaactg gtcaaacatt gttggttaat tgttatacta aatttatcga cataatacta
    54001 tattaattaa aaaagtcaat taatttatga ttatgtcact aaaatgtatc aattttttca
    54061 atattttgta cctattttat caaaatataa caacacataa ctctattcct catctggtaa
    54121 acacctgtag catctatagt acatcatcag gataacctac tctcctgcca ttcctcatcg
    54181 ggtaataacc ccgtactata ctgcaccagc agtatagtaa atcccctaac gccattcctc
    54241 atcgggtaat ggccgtgcaa tctcaggtgt ttttgattca actatcccct tatcacgaag
    54301 aataactatt tcaacccttc tgttataagc tctcccttct ggtgtattgt tatcgtcaat
    54361 tggtctatat tcgccaaatg aaacggctga catctgtttt ggattgaccc ctgctgcctc
    54421 aaccagatat cgcaaaacat tgactgcccg tgctgaggaa agctcccagt ttgtggcata
    54481 tccctcctcc aatcctctcg gtggaacagg cctgttatcg gtatgccctt caatccttgc
    54541 ataatttggc attgacttaa tgatctctgc aacctttttt aaaaccggta taatatcatc
    54601 tttaagtttt gcactgccag gttcaaaata ggcatcgctt gaaagggtaa taaccaatcc
    54661 tcgttcatcc tcacggactc ttaccttttt tgcctgtatc tctggtttaa atgcttcaac
    54721 tgctcttttt aacatctttg ccaatgctct tccctgctca cttgatggaa gattgagcat
    54781 attcaaacca atctcctcca tcctgccacg tgctaaagaa aaaccaccct gcatcatgcc
    54841 aagcgatcct ttaaaagaag ataggatgag attaaaatcc tgcccaacaa tgggggaaat
    54901 atcaaacatc attataaaga atgtaagcaa tagcgtattt aaatctcccc agcttgtgag
    54961 ccacggcggc ggaggtggca attgctgctt ctttcgtttc atcagtcacc cacctcgctg
    55021 gtaattgctg ctctgagttt aggatcaagg aaggatacca atttatcttt gacaatacgc
    55081 ggattatcac ctgattggat ggaaagtgta ccttctatca taatgagctt catcaacatt
    55141 tcctgattat ttaagtaacc caatttagct gaaattggat acaatacaag gtacgccata
    55201 atagctccat aaaacgtggt aataagagct gccgacatac caggaccaat agctgactta
    55261 tcctcaatat taacaagcat aagcaccagt cccagaagtg ttccaatcat accaaaggca
    55321 ggagcatatg ttgcccagtc atcaaatatt tttttacctt catcatgacg cacttgaata
    55381 ttttcaagct ctgtttccat aatacgacgc accaactcag ggtcggttcc atcaacaaca
    55441 agctgtatcc ctttccgtaa aaactggtca tccagttcat ccagatcatc ctcaagagcc
    55501 aggagccctt ctcggcgggc tttttcagaa aatgatacaa gggttatgat gagttcattg
    55561 ggactatatt ttggaggaaa gaaagcatgc tttgcaactt tccataatcc caatgttctg
    55621 ctaagaggat tagagatcaa cagagcacag aacgaaccgc caatcgtaat aaaaaccgaa
    55681 ggtatatcaa aaaatagcaa cggattacct tttgcaaaga ttattcccat gataatattt
    55741 gtaaaaccaa caattagtcc aatgatggta gctatatcca tatgaaacaa ccctcctcat
    55801 agccctttac ataatacagt atatcgtata tataattttc ggttaaaatt ttaaaataat
    55861 tcatcaaaat actatcacgt tttcccggta agtactttat gaaaataatc atagatcaaa
    55921 gataaaacct catcttttga ttccttcacc agatattttt tttcattaac aagggttatt
    55981 attgtatctg gctttgattc tattgtttca atatggttgg catttaaaat aatttcatca
    56041 ccattcatct tatgcactac tatcatgatt gcctcttttt ctttggtttt ttttcaggat
    56101 acagttgatt cataacttta gctattgtca tcttttttac tatattttcg ccacaacctg
    56161 gtacactgca atcatacacc ggtattgtta cctttgaaaa aagatcctcc agccattgtc
    56221 tggcagctaa aaattgatga tcagtataat atatcgactc acggcgttta acaatataca
    56281 ttttcttttt ttgtatcatt gcataatctt gtgtatttgc tgtgataaat ctattatatc
    56341 ccattaaaaa gcgttcgcca accacagtat tgccagcata atagctatgg ttaaatgcca
    56401 gatcattccc cacaatagac agcgatgcta tgggacagcc taacgataat gatatagcct
    56461 gagttgtcac aatgcttgct gttgcaacat atccaagatg ctcccctgcc atcttccata
    56521 aaagattaaa cggttcatca tatatcatcc agttataaaa gcttatcttt cctttccata
    56581 taccagtggt attggcattg acaccactaa aggctaaaag atggcatgat gatgtaggta
    56641 aaaacgcata gtatggaaca ggcattgctt cacatgagat agtaaaatga ggattaatac
    56701 cattgacaat aagtggccta tacgccatat ctgctgcaat aatgataaga tcatttctat
    56761 gctgatattt ttttaaaacc tttgtagcat catccagact tttacctgcg ccacaaacaa
    56821 caacatgttt tccggataat aacgatacaa ccttttgtaa accgccccac tttgcaatga
    56881 gcaccttatt cttttcaata ttgcgctgaa tacaggtata gcgttcaagt ataagcccct
    56941 gattaagtgc ctgataaaga ttttgttgca tggagacaac cttactgcat taattatata
    57001 tggcatcccg aataatggta tgctgcttca atgctccttc aaagacagta gcaacaccaa
    57061 aatatacctg cgtttcccca taatcgccat agcctttcac aactcccaaa aatccttcaa
    57121 tatcaacatg gtagccggcg gtaatgcgca ggtgaagctc tgacccaaag ctatagttaa
    57181 aatcatgtat atcggtttca ccaacccaga tgttgccata ttcggcaaac ggtgtcaacc
    57241 acaaatctct gaacagcagt ggcaccaact tgtaacccat atctttctgt accaacggta
    57301 tgcggtattc cactatagct actgccagcc tgttaccata atactcatcc tttgtaaaac
    57361 cacgcatact gtaggcatcc tcatcatttg gtacgctgtg tcgtttgcct ttttcatatc
    57421 gacctatggt atagggataa agatatttgg gcgcattatg ggcataaccg cctctcagca
    57481 taaacgccag cacatcattg gcaaaacagc gtaaaaagta gtacagtcct gaacgcactt
    57541 tggcatacga aatatccgaa gctaccgagg tgtgatacat atccccgtac aattccacaa
    57601 tcataccatc ttcgggactt atggaataac tcccgacata agttccatca tgtatcaatg
    57661 caacatgacc acctgccagc acttccctgt atcgttgagt ccctgcagta taatagtact
    57721 catcaatgcg agttttttca taggtatagt aaagaagcaa ttgattataa ttataaaact
    57781 ttataaaagg cactgcaaag cctgatgtta tcttttgttc agattcacgc cgcaggtaat
    57841 gtacatttga tgggggatcc catggaaagg tatcatcaaa acctatgcta tcatctttgt
    57901 acgacacaaa catatcaggg taaaaacctg aatagatata atcggcttta acctccattt
    57961 gttgctgggt caatgcatag tcaatctcca gcatatagct gtgtttatac agtgtatcat
    58021 tccccgatgt taaaaaccca gcatgattgt catatttatc tgtgtatagc tcctcggtat
    58081 accatacagg ataccaccat ggcgctatca gcgaatgcaa gggattgtaa gggttgccaa
    58141 tgtgttcatc cgttagctgc tcttcaggga cggtaaaagg ttttggcgat agttctccgg
    58201 ataaggttgt ggtgtatggc atcagggcaa tgttataacc attagcctcg taagatgaaa
    58261 acacgatggt atttccatca ctgctgatat ccgggaaaaa cgctccccct attacattgg
    58321 ttatacggga aattttttta ttggataggt ttatctcgta tagattaaac acaccatccc
    58381 tgtccgaaga aaatacaatt cgtttgccat caggatgaaa agcaggatga atatcagtat
    58441 accgatcatc cgttagcaat actacccccc gtgttttaaa atcatagagt gcaatattgc
    58501 tgcaaccttt attgacaatg gaaaacactg cctgcgaact atcaggcgac acacgaaggt
    58561 atgcaagctg taccggtgta tctttaatac aataatcgtt gatagcaaac gaagcgttta
    58621 accgtaccag tgaatatctt cccttactgt atacaattgc acaatatcca ttggaaagcg
    58681 cctctatata ttgaacacgc aaaccctttg ttttttgccg atagtttgta tcatatacaa
    58741 agacatctct aaaaagattg aatgactggt aatattcaat atcactgaca ataatgcttt
    58801 ttccggcaac acttatgctc tgcgggtcat tgacgctggc aagtttttta cactgttttg
    58861 ttgcagtact gtacagcatc aatgctggtt tttgtttgcc ggttactgtc gcaaaaacga
    58921 tgtcatttcc ataatagcgt ggcatggagg tgtgatagcc tgtactggtt atacgttcat
    58981 agctgctcaa tcctcttttt gttatgctgt caacagcatt gtgtacggca gtatgtaatt
    59041 cctcctgcca cttcttccag agaactatcg cccccgtatt atacacgtca ttaaaattct
    59101 tttccattaa aaagggcagc acattgtcag cattttcctt aaaaagcgca ctaaaatcat
    59161 ttgtttgata gttacgtgaa acataatcgg caaacaaccc accgtaaaga tagggcacca
    59221 tccccgcagg ccattcccgt ggaaaatgtg acgcaagtga tatatccttt acattgttag
    59281 cataaacctc ctggcgcagc accatcatgg tataggtggc atgtaatctt ccccatggct
    59341 tttcctttga ttcctgttca acagcatagc cttcaatagc ccatagcggc atcaacaggt
    59401 tgggaaagca tgcccttcca agcgtatagc gtgtagcctc aggcaatccg cctatcatat
    59461 ccacggtcag gatatgcgta tattcatggg taaagacaag gcgtaaccaa tcatcattat
    59521 ttgatagccc tgcatcttca acatgaggaa ggacaataaa aagttgtatc ctgttataag
    59581 gaaacggcat ggaaaacccg tttgccatat cggtagtgtc gcatagcacc acatctgttc
    59641 taaaaaaagg ctccaagcct atttctctgg ttaaggtttc atggactgct tcggctattg
    59701 gtgcaaggtg caacgcctgc ttttgcaatc catcatggta atgtatccag aaatgttgcg
    59761 ttttgattga ataccattga tagcaaggat gaaacatccc tgcaccaaac accccacggt
    59821 tagttaaaca tagcatcata atagcaatgt ataataaaaa acgttttgac atttttagct
    59881 cacagtataa gcctgatata atttctattg aaattttatt tgcagcaata gatagttttg
    59941 ctgattatgt atatccttta tttgctggca ataacaaaat tacaattata atgtttgata
    60001 gttattatat gaaaaagtgg caggtagaaa gtagagatac agtaagcagg cagacagttt
    60061 tttctgttga aaagctttta tgctatcatc cccaaaaaaa aataaagcac gatttttata
    60121 ttgtaaacaa tcctgactgg gtaaatattg ttgctgttga caaagacggc aatgtaatac
    60181 tggtaaaaca gcacaggctt ggcacggatg aatacaccat tgaaactgtt gcaggtctta
    60241 ttgacccggg cgaagatcca ttgcaggcag caagccgtga attgctggaa gagacaggct
    60301 ataaagctga aacatttata cttttaaaaa aactggctgc taatcctgct attatgaaca
    60361 actatatcta tttttattgt gcactggatg tacacaaggt tgccagccag gcacttgata
    60421 tagcagagga tattgaaata gtaacattac aaccgggtga ggtaatcagc agtatgaaaa
    60481 atggcactat caatcattca atcattgtta cagcactgct cctttttttt atgtcgccct
    60541 attgctctta cccaaaggaa gatatagtca tttattaaga attggcaatg attaaaagta
    60601 tttacatcat agcattatta tttttgcaac ccacatctga tttttcaata acgctgtcgt
    60661 accatccaca accgttttat catcaagaat ggtttataac actggctcgt tcacaatcaa
    60721 aagcttacat tgcaaccaat aacccccaat atttacggaa aaaaagaacg atcaccatca
    60781 atgagtatgc taccatcgtt cagcaattga actcgctggg aatatggcat tgcaaggata
    60841 ggtatataca ggggaattac tatatgctga caattactaa caggaactat cgccaacaat
    60901 gcaaaataga atattcccct tcaatacctg gcaataataa ctattcacag ataattcgta
    60961 taatactgaa cactgcaaat aattatttaa ataactaaaa attcatttga caaactattg
    61021 tgcaaatgga tagatttatt tatctttacg acattcgtaa aaacacaata agattattaa
    61081 ccaaaaagga ggttatatga agattaaata tctcatattg ctattaccca ttatcatgat
    61141 cttttcaacg gtaacatttt ctcaacaggc aactgaagaa aatgttatac agcctgatgt
    61201 acagagccct gtttctcctg aagtaaaaaa ggaagaaata gttacggcag cccttaaagc
    61261 tgtatcgttt aataatggcg ttaacacctt tgtaaattca aaggttaaat ttgaactgat
    61321 tgccaaagac aatattgcca ctgataagat attgtataaa ttagatgccg gcaatgagct
    61381 tgaatacaaa gagcctttta ccatttacga tgaagggaaa cacaccattt catattacag
    61441 catcgataag gttggcaata aggaagacat gaaaactttt aaagtgattg ttgacaacac
    61501 accaccggta gttaatgtag ctgtttctgc accagttatt aaatcgggtg ataagctgta
    61561 tgcatctaaa aactttacct tcactattga tgcaaaagat gctctatcag gtgtacagtc
    61621 actggaatat agctttgata aaaccaacta tcagccttac cttacttcat ttaccattcc
    61681 ggttgaaggc gctattacgc tgagtatcaa agctatggat aatgttgcca actatactga
    61741 gaaatttgtc atgactgcaa aggatgaaaa tggcaatccc attgatttaa ccaatggcaa
    61801 tgttgcagta tacgttgata acaaagcacc tctggtagag atacgtgctg ataaagaatt
    61861 tttagaaaaa gacggcaaaa agattgcaac agttgattac aactacactg tttctgcaag
    61921 cgatacagaa tcgggacttt cttcaatact tgtgcgtatg gacggtaagg gcgaatttaa
    61981 agcttataca ggaacaatta agtttgatac caacggcgaa cacatgatcg aagcaaaagc
    62041 cattgataaa gtaggcaata tatcttcaac tgcagtattg tcagtatttg ttgatgtcat
    62101 tgcaccagaa acattaatta aatctgttga ataatatatt attacagcaa caccatggtg
    62161 cacagcactg tggtgttgca tttatattca ggcactgaac tatgtttatt gatactataa
    62221 aatatgattc aatacttgtt ataactcctc atgaagatat tatacgagaa aattctgaag
    62281 atatagaatt ggctgttaaa aataattata acaaaagtat aaccgatata atacttgatt
    62341 gtaatcatgt tcaatatata aacagctttt cattaggtac tctggtaaag attcaccaga
    62401 acttgcagga aaataattgc aaactacatt ttcttaatgt tgctccttcc attaaaacgc
    62461 ttttcaagat aacccatgtg cttgattatt tttcaatcct gaactcaatt gatgaagtaa
    62521 aagaaaaatc aaaataatat cccggtatcc tttttatttt ttgcaaaata atatttttta
    62581 tagtagtttt gtgaaagaaa cgaattcccc atcatttcat agataacacc tatattatat
    62641 aatgactcaa tatatgatga atcaagggta tatgctttct taaaacaact gactgcattc
    62701 atatagtgtt tgctaaaaaa gtatgcaaag cctaaataaa accacccgtt agccgatgaa
    62761 gcatcaagcg ttactgcgat agataaatgt ttggcagcat tttttatatc accagtataa
    62821 agacatacct tcccataacc atagtgtgca taaaaataat aaggatttat tgaagcagct
    62881 ttcaggaaaa gttcacctgc ttcgttataa ttgttgtttt ttgcatacat tttagcttcg
    62941 ttacataacg aagaggaaaa ttttgaatca tcagattgta gtagcaataa taatgttatt
    63001 attattttat tgagcatcag gaatcatctg gcgaagtgtt tcaaacaaaa atgtatctat
    63061 ctgtttgcta taaaaagtat acaatgtaaa tactatccat accagcaatg tgaatatgca
    63121 cataaatttc agcaggcgtt cagcacccac atcatcagcc ttataaaaga taatgataag
    63181 tgccagaggc cagaggaaaa acatatttgc aaataatagc ccgctgaatt cttttataaa
    63241 ggcgatagtt accatatacc aatctttttg aaattgttta aatcccttca aaaggtcttt
    63301 ataaaaaaac catatataaa acccatatat ccctgatgct gctatcttcc atagtatttc
    63361 tatccgtgga cgcatccaca atagcactac agataatccc catataactg agaacatcat
    63421 catagatgtt gaaagcattg ttgtattcat gctaaacaaa tcctcatttt gatagatagc
    63481 aggctaattc aattgcccgt ttcatactat taaactgagc acaatttttt cctgcaatat
    63541 caaatgctgt tccatgatca acagaggtcc ttactataga tagtcctgct gttatattca
    63601 caccttcatc aaaagctacc atcttgaatg gtattagtcc ctggtcatga tagaaagcta
    63661 taacacaatc gtaggtttgc catcgttgtg gcataaataa tgtatcggca gaaaacggtc
    63721 cttcaataag aataccgtta tttacggcat cctttactac ctttgctgtt acatcggtgt
    63781 caaaggcacc aatagcacct ttatccccac aatgtggatc aagcccgcat atagccagca
    63841 ccggttgctt atgcaatatc tttttgagat acttattgca aatttctact gttgttaaaa
    63901 ttgattcata gttgatatga tatgacagcg tatataccgg cacatgggtt gtaacaagaa
    63961 gcacattgta tcttcctgaa tacatgagca ttcttggatt aggttcatgg atgcgctgcg
    64021 caatatattc ggtatgccca ataaatggca cccatttttc tatcagcgat ttattaaccg
    64081 ggccggttac catagcatcg actatattct ttttccataa atcaatagcc gcatcaatat
    64141 agcataaact ttctttacct gtcagttcat taccatttga aggattaggt accggtccat
    64201 caaaatcaac cgtaacataa cagggtttat ctatagtgtg tattctttgc aagtcattga
    64261 gtgctatgct atcccatgga agcattatat catgataatg ttttataagc acttccgttc
    64321 tgcatataat caggggaaca aacaataatg cagaaaagtt gcttagagct tttaacgtaa
    64381 cttccggtcc aatacctgcc ggatcaccaa tagtgattgc tattacaggt actttattct
    64441 gcaagaaact cctctgggat taatttattt ttttcatatt cactttcaat aagatctttt
    64501 gcagaaattt taagatcatt agctattgtg caacgccctt ccagaatcac accgttttgt
    64561 atgataagct cgggagtttt gatatcacca taaatttttg cggtaggcat caatagtact
    64621 ctgtttgtag cattgatatt gccaataaca agcccctcaa taatgaccga tactgcgtta
    64681 atattggttt ttaccttacc atttggacct atatagagct gatcagcctg taaatacttg
    64741 ccttcaaaac gcccatctat acgcaatgat ccattgatta aaaatttccc gctaaagtat
    64801 gaatcttcac caataacatt atttgcaaca tcaccacctg attttgctaa agccatgaat
    64861 gtacctaccc ttcaattttt ttatcactgg taaataataa ttatattaat gtcaagatga
    64921 aattttactt agaattttgt tttatcaggc actttaatga ttatcaaaac ttccttaaca
    64981 aaagctttta aatggcgcaa agcttccttt accgttaaac cttcaaatgt tttatataca
    65041 tcatatttag catatgttat tatatcatca gcattaagaa ccatggtgcc gttaaattcc
    65101 agatcagcca gtattttggt tataaaatca ttaacatttt taaaaaaggc aaaaagatcg
    65161 attaaaaaga gccttccctg acgagaacgc attaaattta attgagaaat atgtgcactg
    65221 ctgtttgctt tttcaatatc atcaactgtt tgttgaaata tttttaatgc cctctggcat
    65281 tcatccaatt ttctatataa agtatcacgt tccagagaaa ttgaatagtc taattttgta
    65341 tattgatcag ctattactcc ccatattttt tctgaaaaat gttttaaaat actggcagca
    65401 atattaatct cactgtcatc tttaaaattt gatattttct ctatctcaat atcttttaaa
    65461 tactcaaagg cttccagtag ggatttatcc gcaacgggag caacaaggtc atcggtcact
    65521 atagcttcaa tcaattcatt taaatcaaca tcgggatata attcagaaag cttcccttta
    65581 agtgccttta aatcattttt tactcgctca cgctgtttag ggtcagggga tgtttttaat
    65641 acaccctcaa gggctcgtat agaacgaact atctgcctgg ctttttcttc tctttcttcc
    65701 ataaatattt ttctatataa tattcaaaga attttatact atcatagtaa acttatcttt
    65761 tttttgcaac ataatttcaa aatccagtca atacaaagca tatcatagtt aacaattatt
    65821 ataaagtagt atatgatata accgatatta taattacaag gtggtatctg acaattttag
    65881 aggtacctaa acgtaaatag ctataattga aatttaaaat atttttgtaa taatacaatt
    65941 gtggtaaaaa cacttttcgt tgttcaacag ttgagaaaaa ataggttttc gggtatatca
    66001 taagaataaa atttttatat tatatgatgt aactatatga agcttactac tgtatatcct
    66061 gtcatggtat tatgtcttat ctggtttcat aatcatagtt atgcccagta tactatacct
    66121 gtcatcagtg agctttcacg aaatagtcag agtataatgc aaataagaaa ggatatccgt
    66181 caatccttat atactatcaa aagtaatcga agcgttaatg aattacccac tctttccatg
    66241 tataagtacg ttgtaaaagg cagtgaaaac ttctgggaca ttattgctgt aaccaattta
    66301 gatatggata cattgatatc tatcaattca ctttcatcac cttcagatat taaaaaagga
    66361 atatccctgt ttatacccaa tatgcgtggt attatttatc agaaaaatga aaatgaaagc
    66421 tatgaatcaa ttgcagtaaa ctatcgcata ccgcttccat atatcttaaa aataaataaa
    66481 ataactggtt atgaacataa accctacatc ttcataccat gcggaacaat ttctgatctg
    66541 gatcgttctt tatttttggg aactgctttc gtttctcccc taacaacagg aaaactaagt
    66601 tcaaattttg gtgtacgaaa tgatccaatt gatcatatac acaggttcca tgcgggtgtt
    66661 gatattgcct gccctgtcaa ttcaccagtt tatgcatcac gcgatggaat agttattttt
    66721 gccgggtaca aaggcgggta tgggaattta gtgattatac agcattcaag aggttatttt
    66781 actctgtatg gacacttaaa tactattgca gtaacaaaag gaaataaagt tgctaccggc
    66841 catgtaatag caaaatcagg caatacgggc agaaccaccg gaccgcatct acactttgaa
    66901 atcagaaaaa actataagcc aataaaccct ttgttattac tgcggtagta agcctttatg
    66961 caaatagtta aaaacaaaaa tcattattat ttgtgcgata gttactcata atcaatctat
    67021 tgcatcaaga agtgaataca ggtcattgcc atcttcaggg tattccgcca ttaaaaaggt
    67081 aaccagcaac tgcaattctt tttgactgaa ctgttgaaca atttcattct ttaatgcatt
    67141 agcaaatggt acagcaaact tcttattttt acctggtaat acaatcaaaa atttatgtct
    67201 atctatcttg gcagtaaaat caccttccga aagacgattg tgaatgatat cctcaacttt
    67261 atcaatcagc tcttttgttt tattaaaacc ctgtacaata taataccgct tcaaattttt
    67321 tatagtaaac aaaacaatag taagcggtat tgacaatttt gaagcattgt ttaactcttt
    67381 tgatattcta ttaaacaccg gcacaatcac atcgatataa ttggtgtatt gatattcaat
    67441 gagccgcgac gaccaataat gagttaaaat aacatctgac agcgacctga aattatcaaa
    67501 ttgcaaatct ttcaattgat aatcactcaa ccggaaaaca caaacaaagc ctaaaagttc
    67561 atcacttatc tggaaaggat atatccagaa taccgtcatc tttcgcatac agctctctgt
    67621 aaaaacttct ttaatagctt tatttctatc aaaatcttca ataatggtgg gcaggtgtaa
    67681 tgacgcaata taatctaaaa atggagattc tttttttatt atactaactg attcatttaa
    67741 taaaagaaca tcatcctctt cagttaaaag taatgagaaa tccatagttt tattattttt
    67801 aacaaaaaca gcaaagctta aaattccatt tgctttcatc atgttctgta cagttatttt
    67861 aaaataatca tgctgaggat atttgcgtaa actctgttta tactcttcta tcgattgcca
    67921 gaaattttta tcatccagat acctttgtat atcactttct aaatactcaa tttttttaac
    67981 tttttcgtaa gcaatcgctg caaactcact tacctgttca ataaaatcta tctcttcctc
    68041 attataatca ccaattgtta ttttatcacc aagcaccaga gcaccaaaaa caattgcatt
    68101 ataccgtagt ggtacaaata aacgggcatc aatcgctata agctgataat attcatcccg
    68161 atattgtgtt tgctgtttat acttttcaat atcagcgaca ccttttgatt gcattaatat
    68221 cttaaataga cctctggatg tatcaaattt aatattttta ttcttaatag taataccacg
    68281 tgaatctgct actataagag aattttcttc atcaggattc aataccaata acgtagatga
    68341 agctgaacca atctgcccca tcaccgaaaa aagtattgta tcatacagtt cattcagcga
    68401 ttttgatttt acaatttctt tgccaatttc atataaaaca acaaaatcct gaaatttttt
    68461 atcaggcttg cttgatatag cttcagaatg agtctgctct ttatcgtttt catcttttaa
    68521 cgattcatcc atagtattat gagtttgttc cactattctg aatttagtat tattttgaga
    68581 tttgctgctt tctggtatag ttaagtcttc ctgtttcgca cttagtaata catccagatc
    68641 aggttcatct tccggcccaa ggtattcatc ttttccatta atacttgtaa cagtatctga
    68701 atattcactt accttttttt cttcaactgt atgtaatgta ttctcttctt ccatcaaagt
    68761 ttttttatca tcttctggta aaataaataa ttcatcttca acaggctcaa tggttttcaa
    68821 gtcttccctg cttaattcgg tgagcaaatc atcttgaaaa tctaaagatt ctgatttatt
    68881 tttttcttta agtacaagct ctgtatccgc tgggcctgtg attgtatcaa taagcgtctc
    68941 tttgcctttt ttattaattt cttgtttata ctgaagtgct ttttccaaca atcccatact
    69001 ttttatactc ctaattcgtt taacactttt acatacagat caaagtattc tgaattggca
    69061 aaatttttta taacttcttc aggtaattta tcaaataatg aatccagata tttaagtagt
    69121 ttacggatac tttcgcgctt ctcaactggc agagtatcaa caaatctatt gacatcatcc
    69181 ttactttcaa gaactataac ctgatcggta atatttgtat atttttgctg ttcaacatta
    69241 ataccttcac ttttttcaaa tgattctgaa acattttcca acattccatt tacttcttta
    69301 tcaggagctg tgctcaataa attattaata tctatttgcg gttttattgt tttcaaccgt
    69361 tcaactatat catcaatatt ttcttcaata ataactgcca ttggcgactc aatgttttct
    69421 tctatactgc gtttttcttt ttcactgaaa ctttttggtt ttgattcaat atatttgaat
    69481 tgtctgatta atgtctcatc tcgttgtggc tgatctcttc ctaccgcaac atccttaaag
    69541 gtttttgacg cttttactgt ttcatattct ttttcaaaca tgacatcata aaaaaatgca
    69601 agctcttcat tggacaaacc aaatagttca gaggttgatg atcctttttt aacggtttta
    69661 gtttcataat attcatcaat aacctttaaa tacttatcat attcatcagt tacaatagca
    69721 ttatctacaa aaacaatgtc atcatcgcta aacttatatt cttctccaaa ttccaccatt
    69781 ttatcctgaa atgttggaat ggtccccgtt aaaataggag caatatattc ctcttcttct
    69841 ttaaaacctg cctctgttaa tatctttgcc tcaccttcgg taatttctat catcccgctt
    69901 accagattat caatttcatc tattgattta tctgcaaccg gtaattcagt gccacctact
    69961 cctgcatcat caacaattac tatcctttga ttgccaccaa gcattttttg tatatcatcg
    70021 ggtattgtct caaatggctt ggcaggctgc tctgccgtta cctcttttac actatcccca
    70081 ctgagacttg caattattgg ttcaacctta aaagattctt ctgcatgttt tgtttctatt
    70141 gctacaggtt ttattgtact tattgtagca tcgcttccca tagctttatc gtcatcgatt
    70201 agtatttcgg acacatcttc aatgatagat tccaccgttt tatcattttt tgttacattg
    70261 tttgttgatt gaatatgtac tttgcctctt gtttcatgat caactataat tgcggaagta
    70321 ttcccttctt ttattatagt tgtttcattc tttgaaattt ttccagcttt ttcatgtatg
    70381 atcccggagg tttgtgacgc gtcatgttca accttttgtt ttttcacatc ccccattatg
    70441 cttacttcca atgtatcgtc tttgccttgt acctcttttc tcacataatc ttcgctgttt
    70501 atattgtcat ttttactttt ttctttagct gttatgttat ttacagcatt tgaggtatca
    70561 acagggatga ggtcatattc ttcaagctcc tcaattagat catcctcgga aagaataagg
    70621 atttcttcac tggctatttg ttcagcttca tccagattta taaaatcaaa atcctcaatt
    70681 ttagattttt caatctttgt cctcacttct ggaggtattt ctattgttat tacatcagat
    70741 cgtaaactgt tacctttttg actttcttta tcatcgctca ttgctgatga cgcatgttgt
    70801 tcaacatctg tcggtatgtt tttgttttgc tcaattgttt tttgcgatag tttttgggat
    70861 tcactttttt gggataattt ttcatcttct tcaactatat cttcaataac caatgcgtca
    70921 gcagcattat caaaataaat ttttccttta acttccctat tttcgtcatc ataggcagta
    70981 tgaacatatt cttctttaac atttaatgaa ggcatcccgt ttataaaaga ttccattggt
    71041 tcgggtatgt cttcaaataa ctttgctttt tgcacaggtt ttatgacagc aggtttgcta
    71101 cttttattgt taaccctgac ttcatttatg gcatctacat tcaacgttat attaataaca
    71161 tcatcatcca tctcatgcag ttggagagaa ggattttcat cctcaatagt aatggtggag
    71221 gatgtactat ccgttaaatc agttatttct tcaaattcgc ctgaaggttt ttcttcttca
    71281 ggtaaatcaa taacaatatc ctcctctgat atgtcaataa tggtttcagg aatatcagcg
    71341 atctcttcaa gttcatgaac aatatctttg cttttctggc tcatactatt tgcattcttt
    71401 tcttttaaat tttaaacttt agatttcctt atagtatcaa tatatacaat ttttattaaa
    71461 atttctattg cttaccacaa ataatacaat aacgtagtac aaactctcag atatataatc
    71521 aaacacaatt tataaataat tataaaacta ccctactgta caatatcggt taaccatgta
    71581 atattattaa ataccataaa acagcgtgta ccatgtaaat attttgacat tcaaatcatt
    71641 acattataat actatataca tgattatata aaattttttc attactcatg aaaaatcatt
    71701 gcactttgtt gtatagccaa tgtattatta accactatga tagtttccaa tggtattcaa
    71761 taattaatga tatcgtatat cctattatag gtattgtgtc attaatattg ataactatca
    71821 ctaatatttt taatatttta aaaagcaagg agagatactt attatgaagt tatcaaacaa
    71881 attagcatgt ttagtagtag caatggtgct tgtaccatca gtatcctttg cgatagttga
    71941 ttttggtgta tatggtggat atacctttgc tggtaaaata gatagcccta ccattgatga
    72001 tgctgaaact gatggctggc agtatggttt tattggacac cttaacaaat caataatccc
    72061 aatgatactg agtatgggta ttggtggttt ttatcaacgg tccccactgg aatattcaga
    72121 tagcggtaaa gattatgatc taacaaaaac catgtatggc atagatgcaa ttgcaacgct
    72181 tgagcttcct attattcatc cttacctgcg tgcaggcata gccattaatg aaaagattaa
    72241 ggttgataca ccatcaggca cttataccga tgaaaaaaag tttaactcat attattttgg
    72301 attaggagcc gcttttacag tatttccatt tatacagatt tttggggaat atctttataa
    72361 ttattcaaag caggaacacg attcaacatt acacagtaat tccataaata ttggagcgcg
    72421 cttaaacata taacacaact atagctttat ttaaacaact gtgtgatcaa taacaaatag
    72481 tgcaaacatg gtatgctcaa ttgttaatca cataaaaaaa gtattgttaa ggtagcaaaa
    72541 aatagaggcg tgtttacttt tttatagtct tttgtttaaa aactgcctct attagtatca
    72601 tgaaaaaaat tagccatgtc ttatgcgcga attccttcca gataaatttt tattttttca
    72661 aagaggattt ctaatgtttt ttgatttata atttctataa caatattttt tcttcccatt
    72721 tgcttctctg ataatgcttt ttcagatcgc aataaactta atgcattggt ataattaggg
    72781 agcgatagtg actcggaaag caatatctca cctgtatgat acatcctgac accatttttg
    72841 cgtatatcgg ttagcaaatc ttttttttga atccgagtgt gctttgcagg aagtaatgtt
    72901 gcgcatacaa catagtatga ctctaaatat tcctgcaata ttcttccaaa aaatctaagc
    72961 tttgcatcct tttcacgatc cgtattaatc gttatttctt tttgagtgac tgtagcaaaa
    73021 ccagtcatct gcatgtatgt aacaacactc tgtattgatt gcataacatc atcaagcata
    73081 tcctctgaat aaatgaattc tttcatgaac agatcacgaa tatatacata ttcgcttcta
    73141 aatgaatcaa aagaagattg cccattttta tgtaaaaccc gcaaagctaa tgatgaaaat
    73201 gtcagtggca aaaagtaatg aacaattgaa tttttgtaaa aaatcattct tggcctgttt
    73261 tcttccggga gcacatacat atcgggcatg gcttctaact ttttaccttc tccaactatt
    73321 tttaatgtct ctaaaatttt gtcttcttta aacgattgaa taacataatc aatgatctca
    73381 tcaaaatatg atttatcttt taatgactct gacattgaat aatttttata ttcacagtaa
    73441 tcgtataatg actcaaagtt agctttaacc atatcacgtg aaaatccttt caccgatgac
    73501 atgaggatgg cagtagttgt taatgaaaac ggtgtgatag ttacaacatc attaattctt
    73561 cggacaatat tcatggcaac tgcttcagta atattgacag ggttaagctt ttctttaagt
    73621 tcctttaaag taaatgtttt attaaaattt atatacacca taccatattt acggttaagc
    73681 aacttgctgc tttgcatcag gcttaccacc gattcttttt ccttttcttt ccctttgagt
    73741 tcctgaacat agcttgattc ttcaaggata cggtcataat tgattgaaat cggaacaaaa
    73801 acaacatcgg tattataccc ttcatcaata gcttcggtaa tatagcttaa aaaacccagc
    73861 ttgggaaatg ttattttgcc tgtccgggaa cgtccacctt caataaaaaa ctcaacagta
    73921 tatccctctg aaatgattgt ttttatatat tgcttaaaaa tagttgggta taatcttaaa
    73981 cccttgaaag tgcggcgcat aaaaaacgca cccgaatgcc tgaaaatagt acccataggg
    74041 aaaaacgaca ggtttacccc tgccgcaata tgcggcgggc acatcttgtt tttataaaat
    74101 acatatgaaa gtaataaata atctaaatgg cttttatgac agggggtaag caccagtgga
    74161 ccatttgacg cagcctgacg tatttcctct atggctttag catcgtaccg tataccatca
    74221 aatatctttc ggaacatcca gtctaaggca atctcaaata agcgaatgta gataattgaa
    74281 aagttagcag ctatttcttt aaaatattta taggcatctc tgcgtaactt tgcttcagaa
    74341 atatttgctt cagcaacaag acgttttatc tcattttcaa catttggatg atataacact
    74401 ctttccatca tttcgtgctg agattttaaa acaggcccca gtatggtgcg cttttcatga
    74461 taatagtcat cgagaagttt atttctgagc attaccgcca attgacgcgt atcatcaact
    74521 ttagcttttt caatgacttc tttaagatta acagggctgg cgatacgtac aaacggtggt
    74581 gttgcacttt tcataatgga tagccatgca aaaagcaatg ttttatctcc tgttgccatt
    74641 gagcttataa ttgaatataa tggccctaat ggactgcccg aacgttcagg attacgattc
    74701 cagaatatca tctgtggtaa taaaaatatt ggttcatcta tccttttctg aacatctatt
    74761 aaataacgca atgaatcata ttttacctga gtatatcgtt taaggaaata ttgctttgac
    74821 aaaagagaaa aaaggatgct tttattgtcc cttattaaac tttctacata atcagtatcc
    74881 agaacatatt catattgttt tcttaatatt gtagaagaaa aatattgcca caaccttcgt
    74941 gcaatatatc ccatatcctg aaaccagaaa ggattatact ccaatgcaaa aaccggaagc
    75001 ggatatttat tcctttttaa tatattgtac aatataagta aagaaaggtt tgaactctgg
    75061 aaggaagcat acactacatg ccctcttgac atacattgtt caatacgtga tttactttca
    75121 tcatcaaaat caacagtggt aaaaagaacc agcgctaatt tttctactac cggattaagg
    75181 tgctcgtaaa ttaatccatc atatataccc gatgtgataa taccatcacg attttttttc
    75241 ataacaatct ctcctgacaa atgaaggcta tgttctatca tagccatttt tatattttag
    75301 tattttatca ataaattaaa atttgtgatt aaccttacta cattgacaac actaaacata
    75361 ctatatttaa tgtttatgta ttattaacca ctttattata cggatagtat tataaatcat
    75421 acatatttga ttgcaagtat tttttttaag attactggac ggcaaacata aacaatattt
    75481 cataaacatt atacttttac tgtttcataa ctatttatga aagtaagttt cagacaattt
    75541 attcaaaata attattgata atcaaaaaaa tgtttgcaat attcatttag ttttgagtat
    75601 agtagcccga agctaccata acaaattgta atgttttata aaaatgaaca gaaaggaaac
    75661 cgggattttc cttgattgaa ttgtatatca tacattatgt ttnnnnnnnn nnnnnnnnnn
    75721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    75781 nnnnnnnnnn nnnnnnnnnn nnacagaaag gaaaccggga ttttccttga ttgaattgta
    75841 tatcatacat tatgtttttt aattaaattt tttttatatt gttgttatgt tgctacatta
    75901 atatacaaac tattaattgt tcatggatac gggatgtagc gcagtccggt tagcgcgctt
    75961 cgttcgggac gaagaggccg ggagttcaaa tctccccatc ccgaagactg taatatcgtt
    76021 ttcagaggat tacatgctgc attcaaagat gaccttgtta aaagctaaat atcctactat
    76081 taaagaaata cgcaagtcaa ttattgatga tataatgaac cagctaacca tcaataagat
    76141 tcatgtcgca ttagataatc ttgaattata tcttattatt gatgaagcca ttaccaatgc
    76201 aatggagcat ggcaatacat gggataacaa taaatatgtc catgttgcgg tacaggcaga
    76261 tgaaaagcaa ctctatgtta ctatcactga tgagggaaaa gggtttaaac atcctgattc
    76321 acatgataca tcaattttac aaccacgagg taggggtatc ttcatcatga aacaatttgc
    76381 aaaaattgta tggaataaaa aaggcaacag catcaccatg gcaatcccaa cagtatcacg
    76441 gtaaatcaat aacataacta tcccataata tcatgaagcg ctacggcaca actcccaaca
    76501 gattatcaaa tgctacacaa aaaagggtag tatacactgg taataatatt aaacgtattg
    76561 gtataataat agctatggcg atagttatgg taattgttgc aacagctata ataagggatt
    76621 gtaatcaaca aaaaaatgtc tcagccatta aatcagcaaa tattgaacca actgtaaaaa
    76681 aacatgacaa taaaagaaag caaactactc acaaaaatgc agcaataccg tatgaagcac
    76741 ctcgcgacag cagccctcct cagaccaatt taaatatcat taccgattaa atttgttaaa
    76801 gtattacttt tacagtaact atcaaaacaa taaagaaaca cggtacagca atttttgaaa
    76861 tgcaaatgat attcgggcac tcatcccaac aataagaaca attgccagta atccgacact
    76921 gtaaattgct gtaattaatt gcctgtcttt gatcataaca taaagattgt cagcataatg
    76981 cataagcggc gatgtatgtg ctgatttaac cattaattcc ctgtagcatg gtttagtcca
    77041 ccggtatacc ctttccccat tctgataatc actataccgc atgcgtgctg ctataacata
    77101 tttgccgggc ttcacatttg tcaatgtaac ctcacctctg gcatgcgaaa caaaaaatgg
    77161 cggcacctct ttgctttgtg ccacaatcac tgaaaaacca tcaactgctt gagtcggtat
    77221 atgatgaggt accaattgaa atcggtaaaa ttgagttgca tatatttctt gaaacggtat
    77281 attgattgct actgatgggt atattttttg agcaaccaga ttttctatgg gtttttgctc
    77341 gggctggaca attccttttg caagcttttc ataatattca gcatctactt tttcaacatt
    77401 accaataact atctcatagg ttgcgatcct ccctttcata ttggttttat cgataggacg
    77461 caaactgaaa aaatatctac cctgtggtaa attgttaaac ccaacggtat tggctgtggt
    77521 aaaatctgaa ggttctttaa ttgcatcttt tgcaattgaa tagtaaaaac cttttaatct
    77581 ttcatcttca tcaattgtcc actgtaatac aggagaggga ttgtttacct gagtaccttc
    77641 cgggtgagtg ggagagctta ccgttggcat tggcaatggt gtactgctta ccataagcgg
    77701 ataatgaatt gttcgactat aattgcctgc cctatcaata gccctgatgt gaaaataggt
    77761 gattccatct tttaaatcag taactatgta tttgtgtatg ggaccttcaa tattttgtac
    77821 agctggatca aatgatggaa gttcattata aataacagca tatcctgaaa tacctgattc
    77881 atctaccggt ggcacccatt ccaatattgc cgttgtgctg cgtgtccacc tgccctccgg
    77941 atgagtaacc gaatatatcc tgggcggcaa tactgatata tccgaaaatt ttgcaatcag
    78001 ccttccattg ctgttccaga ataataagat gcgttgtcca ctcaagaccg gaaattgatc
    78061 attagagtta acattgggat cggatattct tacttcactg gtaaatgtgt tgtcaataag
    78121 tgaaaatttt ttggcaaaaa cagcttcata acgctctcgt gcatcatacc atgctatgag
    78181 taaatcatcg ttagatgcag caacaactcg tggctggaaa caattaacgt ttacagttgt
    78241 aacattaaaa ggttgagttt cccaattttt tcctttatca aatccacgga gcaactttat
    78301 gccccactga cgattttcat tgttttgata tacacaataa ataacatcat tatgtaaaat
    78361 gattgaaggt gatgaatcat tagcaggact tgttgtgatt ttttttgggt tagaaaaact
    78421 tttcccgtta ttagtagaat ataaaaagaa taaatcatct gacagcaaac caccactgag
    78481 tgttttaccc tgcattacaa tatatattgc attattactg aaagagatag cagggaaaaa
    78541 gatccctcta tattcagatg aaatatcagt tatagcaacc aattgcgaaa aatttaactc
    78601 atcgacagaa tatgcgtgat acagtgttac acgtcctttt actgtaccat gaaaaaacag
    78661 atgtaatccg cctgaagcat cataataaac ctgagaaagc atggactgat ctgatataga
    78721 tgacagtgca accggttttg tccatgtagc acccagatcg cttgatatgg taagcattat
    78781 ggcacttctg ttattaataa gattatgcca tgtacatgct atatggccat tatttgatat
    78841 tgccacatga gggcgtattg ccactgctgg atcaatggta tctaatttgt agggaggtaa
    78901 aaaatcatgt cctgcattta atgatacagt aatatacaca ccgctttcgt tcttttcgtt
    78961 accttcaaaa gcagcaacaa ttacatttcc cttgctaacc gcatatggtg atttaatttt
    79021 cagcgtatag ggtgttatga taacactttt ttcccatccc acaggaaatg taaccacctc
    79081 ctgatagaac aaataaaaac tgccatagat aagcaaaaat acaataacac cccatacgaa
    79141 atatcgcagc tttggtttgc gaaatgttaa ctcaaataaa tatgtaaaaa atcgcttcat
    79201 actcccatta tcggatattt agccggtata ttcacctttt gcttttagct tttccttttc
    79261 ctcagcttca agtacattaa atgctatttt cccaaccttt gtctgtggca attcatctct
    79321 aaaatctatc tcgcgcgggc aactccattt tatcaaatga tccctgcagt gttttatcaa
    79381 ttcctgtact aaatcatcac tcttcttatc cttatattct ggttttaaaa caacaaatgc
    79441 ctttactctt tccacctgtg ctttatcagg tactccaata acacatgcct gatcaactgc
    79501 agggtgttgc cgcaaaacat cctcaacctg tgttggatat acattaaaac cagacgattt
    79561 tatcatcctt ttaagtcgta acttaaagta gaagaagcca tcttcatcca ttgtgccaat
    79621 atcacctgta tgcaaccata ccaaaccatc atcgtgtttg cgtaatgttt tagcagtttc
    79681 ctcaggttga ttgagatatc caatcataac agcaggacca tgaatacaaa tttcaccttc
    79741 ttctccccat ggagcctcct gaatagttcc agtttttaca atctttgcca tcatatcagg
    79801 aaatgggatt cctatgctgc cctccctgta ttcattcatc ggcattgcca taatagcagt
    79861 tactgactcg gttaatccat aaccttcgcg caatttagca ttccctccct gttgtgctac
    79921 aactctttca aactcatctt tgacaaccct tggcaatgta tctgcaccac tgaatgcagc
    79981 ttttatgcat gacaaatctg ctgattgaat aaccttgctt ttacttaatg cttcaaataa
    80041 tgttggcaca ccaattaaaa agtttggctt tgtttttttg atcaattttg caataacaac
    80101 tgcatcaaat tgaggcacca gtatgctttt accaccaccc ataaagaaag cattaacaca
    80161 aacacctaaa ccaaatccat ggaatattgg taaaattgcc agcactgcat caccctccga
    80221 catcctgccc catgctgcta cctgctttcc ttcgctgata aaattcatat tggaaagcat
    80281 aatgcctttg ggcatccctg ttgtaccgcc actatagagt atcacagcca tatcatcagt
    80341 tttcatattg gattttggaa catcacctga agattgtttc attacatcat tccaccatac
    80401 aacacgagga tcttgtggta cctttggtat ttttctaccc tttgttagct taaatcccaa
    80461 tgctttaagt gccggtaaat aatcagaaat ctttgcaagt atcaatgttt tacatggggt
    80521 tttatccata atttcattaa atgcattata gaatgcatca atcgttaatg caaatttgct
    80581 attgctgata gtaagataaa attctatttc ttttggtggc gatagtggat gaatcatact
    80641 ggcaacagca ccaatcttat taacagcata aaaacagata atcccctgag gcactgttgg
    80701 catcgagata gttattgtat caccttctcg taatcccaat gcatataagc ctctggcaca
    80761 ctggtcaata agttttgaaa attccttata ggtagcagta tgccccataa aatcccatgc
    80821 aatagcattg ggtttacgac ttaccgtatt catgagtgct tcatacatcg taacttttgg
    80881 atattctaaa gtatgaggta catcaccata aaatttaagc catggctttt tttcgtacat
    80941 atataatctc ctgcaaaaat ataattattg cttttcgatt cgatcctgaa tcaaggttca
    81001 gaatgacatg ctacgtttcc gggacgacat tctcaatttc aggatgacta tcgccaatta
    81061 ttttcattcc ttgttggtag ccataagcaa gccatcgtta actcccctaa caagaaaaag
    81121 atgagattct accttctttt attcttaata atttccataa atgcttcaat tctggtaaga
    81181 atctggctct cactgaaatt acgttcatca agcatatctg cctcaataat gagtgaagga
    81241 atattacgtt tttgctgcac aatacgctgt atatcatact gtcccagtga atacggttta
    81301 cagcttcggt tagaatgcat aaccagtcca tcgacatcat atttgtcaac cataccaata
    81361 actgcatctg ccatctgatc cactccaata ttaagataga tggctgtata tccttctatc
    81421 atgctcttca aaaaatcatt ttcatccaga tagtctaatt gtctgcacca cgctgttgta
    81481 taggtatccg caacaaggca ggcatcatgc gatgcaaagg tttccgagag ccatttcgtt
    81541 ttataccata caggcagatt atcccacaat aggcggtatt tttcattggg tattgcactg
    81601 ataccatgag cattgcgctc cttcatctcc tgtaaaagtt cagtatagta atccactgct
    81661 atttgtgtgc cgcgtaatgt aacaataaga gccagatgga agaaggcgtc aaatgcactc
    81721 atgggtgcag gtttgtgcat ggttgtatca agtaccgcct gccataaccg ttgcccttga
    81781 attgaaagtt ttccaacttc tttagcttta tcataatcaa atttcttacc acaatgttct
    81841 tctaaaaagg taatgtactc aactatctgt gcccctacat aatctttggc ttcctgagtt
    81901 attcccttat gacaaaacgg cgtatcaaat ataaacagcg gaaccttgaa ataacgagcc
    81961 tgcacctcgt accatttgag caccgtaccg caaatattat tgcaacaaat caaaaaatca
    82021 ggctttggca aaccaccaat tggaccacca ttaattggtg cagcagcgat atcactgcgt
    82081 gcatacgagc acaggtcacg gcaatagccc atttcctcag atttttcaca caactgcaca
    82141 cccatttttg cggcgccaat catcgcacca tgattttctg gatataccgg tataacatcc
    82201 attaccagaa gaggttccac agggcccccg cttgtaatcc atccaacctt cttattattt
    82261 tgagcagcag tctttgcatc aatatagtaa ttggtcatta tctctttcat ccgtttaacg
    82321 gattttattc tgttgtcctt tttatcctgc atttcacctg acatagtatc acctcacttt
    82381 aaatcatatc aataaatgct tcaagtcgtg tcttcatctg cccttctgac ggcaattgaa
    82441 gctccaattc cagcaatgtg gacggaatac ccattgtatc cagtgcctct ttaatatagg
    82501 gataatcaaa cgagtgagga tcacaaaatt ttagatacaa aaatattgcg ccctgaacat
    82561 tatgttcacg taccattgat gtaattgtct caacacgggt attcagatct ctatgcttgg
    82621 cagggcatat attccgctca taataccggt tggcaatagc ctgcagtgga ttgctatcgg
    82681 ttgctatctg cccctcaaaa taacgagagc cagtacacag atcatcccac accaccacgc
    82741 caccggaatc ttcaatatag gtataaatat caggatgatt gcatataccc ccaataagca
    82801 ttatgcgttt tgccttttca tttttgggcg atagttctat tgaagggatt agattcttta
    82861 acatacctgc aagttcatca cggtcccaca ccattgcagc tttaatcagt gcattcatct
    82921 gactgccatt caacgtactt ttattatcac tcttaagctg ccagatagtt tttaaaagcg
    82981 aacggatttc attataggta ctgatagctt tttcaagctt ttcactggtt attgttatgc
    83041 caaatgcatc ctgtaattct ttgacaaact ttgccagcac atcatacata tattttttag
    83101 cgctgtcagt atttaatttc actgcccata caacatcaaa atgatgtttc ataccggcat
    83161 ttaagcgcca gacatcagat aggcgctgta ttgaatcgca ggtatgggga aatactgccc
    83221 cgtcaacaaa tgataatttt ccactcagtg catcttcaag cgcaccacgt accagtgaac
    83281 agctatacac ctgtagatat gcatcagcct ttgcaatagc atcaaccatg ccgaaaattc
    83341 tgaagggatg aatatccgct gcatagatga tctcttctgg tgtatatgaa cacatatatg
    83401 ccagcactgg tttgcctgtt gcttttttca tagaggcaac atactggtat ggatttaggt
    83461 cttcaaaact ttttatgata tccatgcaaa aaacctcctg aaataataga ataattcaca
    83521 atttttgggc gcatcacaat gtgtatctgc atactttacc actgccgtca tttcagacct
    83581 gttccggaat ctcgttcgac ctcaccccta acccctctcc ttcgtaaggc gagtggagta
    83641 tgggcatctg ccgtcattcc aggcttgatc cggaatatct aattggtgca attgcagtgc
    83701 attaactggt aactatcata cagaaacaat tctttacagt aatgtataat gcaacgacac
    83761 ttccaaacat agcatactat cgtataaaaa aatggttgac agcgtacgat aatgtcgtat
    83821 actgtacgac atatatttca atataaattt gtatatgtat actgtcaaca attttttatt
    83881 atatagtatg acaaccatga aaacaaaaaa agttaaactt atccagtcaa ttgcaaaagc
    83941 acacgacatc ttatcatttt ttgtccatga taaaaggacc attggcatca gtgaatttgc
    84001 caaaaagctt tctttgccca aaaccaccat acaaagtatt gtaaaaacac tttgttattt
    84061 gggatattta gaaaaagatg cctcaaccgg caagtacatg ctgggtccgt atgtattcca
    84121 gcttggaatg aaatacgcaa ccaatttaga tattgtcagc ataggcagag tgtgggcaga
    84181 gcgtctttgc ttccaatatc gacaaccggt aaatataggt atgctggttg gcaacaaggt
    84241 tgttgtagta ctccgtgttg aaccggaaaa tagatttatg acatacccgc agacgggttc
    84301 ggtcattcca atgcatacaa gctgtattgg caagcttctg tgtgcatata ccgataacta
    84361 tcggttacaa gaattgctgg aaaactatga attccaggca ttaacaaaga attccatcaa
    84421 tagcagggaa gatttcttaa aagaattgca atcagttcgc tctatgggca tcagctttga
    84481 taaacaggaa tcaattgtgg ggcttgcagg tattggtggg ccagtattca actataccgg
    84541 tcagattatt gctgcatttg ctataaccgg cgatgcagat tttattgaaa aatataggca
    84601 tgatataatc acaacagtaa aggatacatc attacagcta tcattacaat tgggttatat
    84661 cccggccaat catcaataaa atacttgacg gaaacaggag acagtagtat attagtatta
    84721 gcactcatca gtggagagtg ctaataacat tctataacag ataagctttg cccctctaaa
    84781 aaaggggctt tttcttaaaa ccctccgcca tacaggtttt gattatactt aactaaatca
    84841 atagcaaaat tttttatagc cttttctcta ctatcagcct gtgcagcata ctgtgggtca
    84901 gcagcaacaa cagcattttt caacaaatca agaaaacgtt ttgtatcggg cagtaaattt
    84961 tctgtataat agtagtattg tttccactgt ttttcttccc gagcacattg tgccatatat
    85021 tctatcatat acaatgaacg gagcaatcgt tcataaacat atactactgt atcaggtttt
    85081 tctgtatact tttgtgcaat atatttatta aaagctgcaa cctttttatt atccaccggc
    85141 tggttattcc agaaggtaac atacacaact tcaacaatat catcatatcc caatgaagcc
    85201 tgtaccatat cagttgctgt ttttatgaac gcaatctgct catcattaaa aatggtattg
    85261 atatccacag cataaccatt ggtaacaact atcatacaat ataccacaaa aaccaatctt
    85321 tttatcatac ttatactatc acctttttag tttatgtcac aacacaatcc cgggataaca
    85381 gacggtacat accatcacta ttttgtgtca atcactattg gttatattct tcaaagcttg
    85441 tgttagctct tcctgttttc taaattcatt ggcttcttct attgttttaa acaaaaatat
    85501 accgcgcctg cattgtattg gatagagctt tgacgacagt aaaaacaatt gtcgcacctg
    85561 ctcataatac tttgcatcag gattaaaatt ccataatgct cgttgtgctt catcaaatgt
    85621 tttatattta taaatcatgt ttcttctttt tcattaannn nnnnnnnnnn nnnnnnnnnn
    85681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    85741 nnnnnnnnnn nnnnnnnaat atcaatatct tgagtcagtc gtggcatgcc atataaaatc
    85801 accgcatatc cgccaattaa aacatagtca acatcttctt tattcaatgc ttcaatgaat
    85861 gttatgaatc gttcaaataa atccatatta atatctctaa taaacggatt atcaatccat
    85921 atcgttttca aatagtggca tatcttttat aatatcccgt tttatttttt taccaactat
    85981 tttgtccagg tatagtggcg atagtcccac gccaggccgt ttcagtgtta tatgttcttc
    86041 agttactatt gttccctttt taatatcctg cgctgcaaat atactgcgac gagcagagcg
    86101 agctacatca gcctcagcat cgtgataatc tattgcgcca tcacccatca tggtataagc
    86161 catatgtata tcgtcaacat agcgtttgaa cgcttcaggc gataatgaca catccttatc
    86221 aacgcagtca tgatcattgg acaatttaaa gtgcttttca aataccagtg cacctgatgc
    86281 cactgccatc atggatgcac gattatcatt ggtatggtcg gaaaatccaa cgggcacacc
    86341 atacttgtgt tgtaaatgct ggatgcgcaa caaattgatt gcatggggag gaacgggata
    86401 caatgatata cagtgcaata aaacaatgtt gttaacgccc ctttcctgta aaagttttat
    86461 agcatggtca atctcgtatg aatgcgatat tcccgttgaa catagtaccg gttttctggt
    86521 tgctgcaatg tttttaatca agggcacatt ggtaatttcg gatgaggcaa ttttatatag
    86581 ggggacctgc aattgctcaa gcatggcaaa tgatggcaga tcaaaacagg atgcaaaaaa
    86641 tattacacca tcctcttttg caatttgttg taatttcgta taatcacttt gtgtaagctc
    86701 aaattgttta aaaaaggtaa tcatggaatc atcataatgc ggcggttgat tgttgataag
    86761 cgagcgggta tataccgagt ataacagttc ggcattgatt atctgaaatt tgactaaatc
    86821 cgcaccggaa tctgctgc
//
LOCUS       DAOOJH010000002        71226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_424_length_71226_cov_10.112785, whole genome shotgun sequence.
ACCESSION   DAOOJH010000002 DAOOJH010000000
VERSION     DAOOJH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 71226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71226
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_71226_cov_10.112785"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="PLJ75_00405"
     CDS             complement(<1..303)
                     /locus_tag="PLJ75_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03712.1"
                     /translation="MNTSDLFNAPKNLLDGKYRITKKTQTVFFGEYYEAVNIKNENVY
                     RVCFINKKNIDKYSVIKFHNSLNKIINAKIPSIVPIVSTGELLNYIYVITEYPEGMV"
     gene            complement(305..1693)
                     /locus_tag="PLJ75_00410"
     CDS             complement(305..1693)
                     /locus_tag="PLJ75_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03713.1"
                     /translation="MHITITRKNSINVISLALLLPALLMSLQCNKGNDILATYNGGAV
                     TRQEFMDWLELNYYKREVSHIMKYKTLRIQKVQEMCKEKILILEAKKQGYDKQPYFSN
                     IKELYADQEIMKYFLKKEIEEKAKIKEPMVHILAFYINDVTDDSYKKAVNVIATCKSK
                     SFLDVYKQYSNNQKDFMCMDFRYTFLFMLPESLQKGIEKLAKNDYSMEPIIVKDGNDW
                     FNSGIYIVFKKDERTATLKNLYFITNDKKEAHDLKNIVLNRLLHEYIDNLRNGKDVYF
                     NYNALDSNTVNTILFTIGNKSITVHDFNKRIQKLIESKIQLGSDAAIANEMTKKSFKI
                     EYAWEYFTNELVKRVFYEKGYHNEPEFVKSLNNKHDMILEIEFKKHLTPKNVNVSLAE
                     IKEEYELTKDKYYAYTETVNGKQIKKYIPLEKVKDEIKKILENQKKEDFINKYKSNLA
                     LQYNVTLKTESI"
     gene            complement(1734..6902)
                     /locus_tag="PLJ75_00415"
     CDS             complement(1734..6902)
                     /locus_tag="PLJ75_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD03714.1"
                     /translation="MEKILGMQIIQKIAESPRTITYRIEYKDSTAILKVFNIKQYSLS
                     DFIRFKQEYEIIMNPDISGIVKPYEFIDTHEGFGIVVEDFDSISLKDLLMKKKIVALN
                     EFFTIAIKVSQIVGTFHQYNIVHQDIKPSNILLTEDLHTIKITDFGIRTLFPNEYQEI
                     YRKDIMEGTLLYMSPEQTGRLNRNIDYRTDIYSMGITFYELLTGNVPFKSDDPIEIIY
                     AHIARKPVPVSEVNPHIPGIVSQIIDKMLAKTPEERYQNSFGIVGDLIQCNDEYTKKG
                     TISPFKIASFDIPLRFNIPQQIVGRDSEIQSLLSSYKNFMEGHREIVIVKGSPGIGKS
                     ALVNEIYKPITATKGYFISGKYDQFKRDIPYSSIIQSFQMLIRQILTEDLSKLELWKE
                     ELLNAFGKSGKILTDVIPDLEHIIGPQLPVEQLSPEESQNRFNYLFKKFINVFAVQEH
                     PVVLFLDDVQWIDLASLNLIRSIVLDESVKNFFLIMAYRDNEIDASHPINPVMHEIKD
                     NGVTINIIALAPLKEEDVNTFLLNFLSNNVESTMELSSLIYSKTAGNPFFIIQFLKRL
                     FEDKLITFSPEKGWIWNIEKVKELPITDNVVDMLSQSINKMPAENIELLTICACIGNR
                     FELEDIIAITGETAEAILKVMNSLIEFGFIGLFKNIYRFYHDRIQEAAYSLLDEEERS
                     KLHYKIGKNSLSKTAKENIQDKIFYIVYHLNKSVHLIQTVEERIQLAELNLIAGEKAK
                     NSSAFDSSLIYFKQAAELLPENAWIDYYRLTFKIYLNYAESEYLAGDQNNAATLFDVL
                     SHYAQSRLDKAEIYINRCLFLTNGGQYEEAVRLGLEGLKMFGISIPEKPSMLNVIKVM
                     LALKIKLQFIKPEDLLQLPWIENPEEIAVIRIFESVTQAGYYTSRNLVIIGPIILFNF
                     IIKTGNCYLSSYLFSVLAGIVGPGLGDYKWANQFYQLSMKLLDKTPEKNACSVYFTLA
                     YLYLHWNAHVQENIVLFRKSYNMFLGLGKFDFAAHSINNIADYSFILGKNLDEIFTEY
                     KNYEKFFKNQKDKFFHSYYEDNIRKYYCYKGLTDSPSTYNIHDNYTEERLQAVRQTKN
                     NLDKFIFVFNLMKIEFLYRNLQKGYEYAREIEPIKNELVGNIHYPEFVLYYLLLLAGL
                     RKDKKISKIIWAMKSKLLVSMMKKWSTVCPFNYLHKYYLMLAEKYAVTKNYKKAKEYY
                     DKAISEANRNGYIFIEAIAHECLGSMYISLNDKTNASQQLEKAYNLFHQCGADAKATM
                     LHNNYRDKIKIEQTRTAIAIDSDSSSIASSLDIVDFSSALKASLAIAGELNFEKLPGK
                     LLSIMIENAGAEKGFLILKDNEDLIVVARKNTIDEEITALENLPVDRVDGFSHAIVRL
                     VARTGEKVIINDAMNDPFYVNDEHIFKNKVKSLLCLPILNQGKVVAVLYLENNVITAA
                     FTQRHIALLTLISSQAAISIENAQLYLNLEKKVEERTKELKEARDALWGEMQLAAKIQ
                     TVLLPKSPMAKGYEIYGYMIPATEVGGDYYDVINTSTNDWIIIGDVSGHGVSAGLVMM
                     MVQTAIQTIIRANPNLTPVTLLKVVNRTITYNIKQMNEDKYMTITALKLYPEGKVEYS
                     GLHQYILVYRYATKQVDRMESDGLWLGFDIDLNRDTSLKEFTMNKGDVFLLYTDGITE
                     AKTDNGEMFEEDQLANLLQNSGEKPLAEIKNDIIAALRNYHTNDDVTMVLTRKV"
     gene            7300..8484
                     /locus_tag="PLJ75_00420"
     CDS             7300..8484
                     /locus_tag="PLJ75_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HPD03715.1"
                     /translation="MSYPLHDMKILDFTYLLPGPYGSMMLADLGADIIKVENPHNPDM
                     LRMLPPIVDGISAVYAHLNRGKKSLALDLKAPDANNIIYKLVKEYDIVIEQFRPGVLE
                     KLGFGYEDLKNINQGIIYCSLTGYGHTGSYASRAGHDINYMALSGVESFSGKKSTGPS
                     LHGIQIADIASGAKNMVIGILAAYIKRQKTGNGDYLDISITDGVFAMSTFTTAAYLEG
                     DVLPMPQAQILNGGSIYDYYMTADGRFLSVGPIEPKFFQSFCDAIGYNDMATTMDFSL
                     ETVQKHKRDIAVIIASKPLSYWIDVFSALDACVEPVQTLDEAINNPPLYDRDMIVNVT
                     TMNGKKLKQIANPIKFSSDTYIAQYAGVSLGYNNDEILHAIGYNENEIQQLYDNKTIT
                     KK"
     gene            8502..9821
                     /gene="miaB"
                     /locus_tag="PLJ75_00425"
     CDS             8502..9821
                     /gene="miaB"
                     /locus_tag="PLJ75_00425"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPD03716.1"
                     /translation="MKTHKTFTIITFGCQMNKSDSELMELSLLKSGFHKSDSYHSDIF
                     IFNTCSVRKHAEDRAIAHITETRSKYKDAIIAVAGCMAQRIAHELITEFGVNLVVGPY
                     QSPDMGNIISRYLYNSAINKYISQNADDFASRLDNELAHHSDELPWHKWVTISHGCEN
                     YCAYCIVPYVRGKLISFPSQQIIDYCTVLIDNGIREITLLGQNVNQYGQDSGDIPFYK
                     LLDKVASLRGLYKLNFITSHPKDFNDNILHVMNNNPVIARSIHLPLQSGSDAILQAMN
                     RQYTMKQYFTTIDAIHTIVPHCSVSTDLIVGFPGETDKDFEMTLNAVKTMQFDEAFTY
                     KYSPREGTRAYLQPDTIPDNEKQRRLDILITTVREVSLKQLHNHINSTTDMIVERISK
                     KSDSEVMGKTICNHPIIIKGTMDDIGKKYTVTIVSLKGSTLYGERTA"
     gene            9802..10920
                     /locus_tag="PLJ75_00430"
     CDS             9802..10920
                     /locus_tag="PLJ75_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPD03717.1"
                     /translation="MASVLRNIIIVLLCTVTVAASEQSDYEAIQKAFSSNAAYADELV
                     KKFFTHYPESKYIPDIRMLVAASKKSVNASVADYQKIIQLYRYYNKRDEAFFSLCQLY
                     YVKSDWINLLSTSNRAIAECSTSNYLPVFYQFRAIAHFNYSNYENGLSDCETIAEHTH
                     EINALGYGLFLKTEMEKNITGYSRKYIQGITEILHGYDSTAIYPSVVLRLGQYYEQHK
                     MYNHAWSAYQYIINNYPKAPEAVTAALYAEHIKRYNPVSVQFMPDESIIADSTSIDIA
                     PDVNIPDSNNHTVHYAINIGPLYNLAKAKELQKLILKIVMPVHIARKTQSFEIYAGHF
                     STFDECMNVKIQLAEELGINGFIIEMVNQDGKNYIYGQ"
     gene            10910..13477
                     /gene="mutS"
                     /locus_tag="PLJ75_00435"
     CDS             10910..13477
                     /gene="mutS"
                     /locus_tag="PLJ75_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPD03718.1"
                     /translation="MGNDNDKELTPMMRQYLAIKQNFNDDILFFRLGDFYEMFFDDAK
                     IASKILDIALTSRQNDIPMCGVPYHAADSYIARLIKAGYKVAICEQMEPPSSSTIVKR
                     EVIRVVTPGTVVESNLITSDENNFLGSIVIVNNTVCAGFVDVSTGDFFITTADYSNEF
                     ICTEITRFYPKEMLLYAPQQQMNYLKELLKQQDITLASINEWYYDTEYLTNIITDIYR
                     LTNIKGLNLGTIEILTVGSLLHYLKDTHKSALSHLKFPKNLSRSDFMVLDHQTINNLE
                     ILKSQDGSRLHSLFGVLNYTQTAMGKRALERALLYPLLDKKAIESRYDIISYFINNIH
                     LLKEIQDVLSSIHDMERLLSRFTLKKYYNRDFIALQQSIEAAISIYTIIGQHPDALFN
                     MLGSISPHVQELSATIKNTIVDNPPLSSEHGRVIKEGVNAELDRLYALKTDAKKWLLQ
                     YQEDEKKRLDISTLKVRYNKVIGYFIEVSKGQTSKVPDDYFRKQTLINAERYTTQTLQ
                     KFETDVLQSTDSILNIENDIVNSLTNRIVELKNHIQDLAYNIGIVDLFCSLAQAAYHN
                     NYTRPALNDEGIIAIINGRHPVVEKFYIKEPFVPNDIFLDNTDNVIAIITGPNMSGKS
                     TYIRMAAIIQLMAQIGSFIPAQSGNCCITDRIFTRIGASDNISRGESTFLVEMNETAI
                     ILNNATDKSLIIMDEIGRGTSTYDGMAIAWAVVEYIANYIKAKTLFATHYHELTTIKK
                     KGIVNYTVMVKESINEVQFLHKLIPGAADKSYGIHVAKLAGIPKTVTIKADKILDKLE
                     KSSRVELLAKMPDNEDQLEIFQATHHRLIQALNEINIENLTPLDALNEIARLKRLIE"
     gene            complement(13496..14083)
                     /locus_tag="PLJ75_00440"
     CDS             complement(13496..14083)
                     /locus_tag="PLJ75_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD03719.1"
                     /translation="MPKQKKRFRDKDHHVVDAYYDLCEEYDGRNAKSITTKVKKLIEK
                     DPDYFDTYLFLYDILLNEGKISQADTILNTAYERAIQLIVDDKGNWPEVLEWGWLENR
                     HIIRTILTKAIDLWDKGEIDSALDLLRKLLRSNPNDNIGARNYILAIRMNMTFSEFEH
                     RFNKGGFYDNKMFDWFEKNYKKFPDEFDWWDEMTQ"
     gene            complement(14318..15082)
                     /locus_tag="PLJ75_00445"
     CDS             complement(14318..15082)
                     /locus_tag="PLJ75_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPD03720.1"
                     /translation="MFQALPTLILLLVLLVVASIKIIREYERAVVFRLGRLLTPPTGP
                     KGPGLVIIIPFVDKMLRVSLRTVAMDVPPQDVITRDNVSIKVNAVVYFRVIDPNKAIT
                     EVEDYLYATSQLAQTTLRSILGMVELDDLLSDRERINHELQKILDQQTDPWGIKVTTV
                     EIKHVDLPQEMQRAMAKQAEAERERRAKIINAEGEFQAASKMVEAAKLMEPHPIALQL
                     RYLQTLREVATERNSTTLFPIPIDLVEPFLAHKKKL"
     gene            complement(15208..16527)
                     /locus_tag="PLJ75_00450"
     CDS             complement(15208..16527)
                     /locus_tag="PLJ75_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPD03721.1"
                     /translation="MKIRSLIIALLGVVLLTQFVFADEKYALIELNGSVNPVVADYIV
                     NSIKKANQDNMQFVVMVMDTPGGLLNAMRDIIKAILDSQIPVIVYTYPKGAQAASAGG
                     FIMISAHIAAMSPGTEIGAMHPVSPFLNFSQDQKSDGIMEKKVLNDTVAYARSLAQLR
                     NRNIAWVEKAVREAISSTNNEALRLGVIDIVANDIDDLLKKIDNKSVKIKDKFIRLHT
                     HNIQQVQYTMNWKERVLNYFADPQMVFFLFIIAVVGIGFELKNPGMIVPGAIGAVAFF
                     LFLLAVKVLPINFAGLTLIILAIVLFILELKFTSYGLLTLGGIISFILGSMILFDSPL
                     PGGQIPLTSVFTVLIVLLLFIFIVVRAVIKVHTKKAVTGIEGMLGEKGYATLDFTGRG
                     AVQIHGELWNAYCDVPVKKGDEIIVKRVDGLTLYVEKVSGEQRIRSQ"
     gene            16721..17965
                     /locus_tag="PLJ75_00455"
     CDS             16721..17965
                     /locus_tag="PLJ75_00455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD03722.1"
                     /translation="MRVYDDTLYFNHGTLYNMRQTIVIIAIIALIGTISLFAQTVKDS
                     TPVDKPDNTKTPDVTANEQSTVTSKSKTISNTPEQDKKNTQQTATTKQKSDIKQDKNP
                     EAEKKKVEWIKNTMRYGIHSDRIDAIKSALTLKEQSNKDDVMKLLNELMPQETNPEVK
                     RTAITIAGELKSTSSVSWIINSLNNQSEEVRIEAVYSIERLKATEAIPKLVSIVKEQN
                     FTENSNFTEALINTLGKLKATELKDFAIERIKETKTTDNLRQLLVLFLGEIEDISAKD
                     FLLQLLKDKDENPTIRSYAAVSLSKIGAKDTANDILEILSEIDSYPLNKRKDYYSLSM
                     YCTASLVKMGDSNAIPRLMEALRSDNPVVRLKAVELMKDLKDKRTVDILKYKMNYDPS
                     PKVQKAAREALKAMGEEVDESK"
     gene            18366..19304
                     /locus_tag="PLJ75_00460"
     CDS             18366..19304
                     /locus_tag="PLJ75_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPD03723.1"
                     /translation="MPLVSDFDKYFLHLPRISRPDDFDDFWHTAINELKKTPIDVQFI
                     HNKRKSTGKFTAYDMTYIGYNKVQLFASLHIPDKINKPHVVILFHDYDDVTSHAQFPI
                     DLPFAYCFVELRSHRNIIHYEEVEKKSPGFMVEGILEKNDYYVKGLYLDGLRTIDALR
                     LHNDLDCSRIAIVGKGLGAAVSVFTASHSNRVVAMVLDTPSFCYIDMAQNIAKSTIAN
                     EINEFIEEHKNKKSVIKKTLAYFDALQFTNKISCPVLATVGFKDTLSPAECVFALFNH
                     LQCDKTIEVYPEEGHIPGGIEQMNRMIEWIKNQFEK"
     gene            complement(19311..20888)
                     /gene="murJ"
                     /locus_tag="PLJ75_00465"
     CDS             complement(19311..20888)
                     /gene="murJ"
                     /locus_tag="PLJ75_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPD03724.1"
                     /translation="MATSTQHDKSFFNTLMVATGIFLSRIAGLIRDRVFAHFLGNSDA
                     ADAFRAAFRIPNFLQNLFGEGVLSASFIPVYAKLIAQNKHDEAAKVAHAIAGILSLLI
                     STLVLVGIIITPLLITVIAPGFEGQKRELTITLVRILFPGAGVLVFSAWCLGILNSHG
                     KFFLSYSAPVLWNVVIIITLILFGDSSSQDELAKYAAAGSVIGSLLQLLMQLPVVIGI
                     LHHIKLSFTVKTKEVKTILKNFVPVFISRGVVQISAYIDALLASLLPTGALAGLSYAQ
                     TLYMLPISLFGMSVSAAELPAMSKVSGNTRDVAKGLQKRINNSIKRIAFYIVPSTAGF
                     FILGDCIVAAIYQTGKFTTRDTFFVWMVLAGSTVGLFATTQSRLFSSAFYAMHDTKTP
                     LKCALIRVALSSTLGYCASQYAPYLLHIDKLYGTVGITVASGLSGWVEYRMLRYTLSK
                     VVGKVALGKRYIVSLWVSALIAAGVAYLIKSVLHYHPITIAFIVLSLYGMIYFALSLL
                     FKIDEMKNLLSMIGIKK"
     gene            21262..23232
                     /locus_tag="PLJ75_00470"
     CDS             21262..23232
                     /locus_tag="PLJ75_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD03725.1"
                     /translation="MNRIIIYTCFIVTIATIAFAGDWPIYKGNLFFTGNNDEIIVPGN
                     QIKWLFLAKDKVFNPVVSDGRIYFVDKNAFLYCIDEEAGTILWLNDVHAISAQFKAYS
                     KSAGKIKYPLVYGNTVLVSDPIAIYAFDKTNGKVIWARTGLRVDETKPQGLTGIATQV
                     MVDGIYADPIIDADTIYYGTRKVFASRDIANGHIVWENKDIATYSGFPVYYDQYIITQ
                     SMNYETKAFTVFCLEQRTGKPVWQRTFSVPIKILPPVVNKNVYVPVNDTIYCLNIKNG
                     EIMWQKSYNRTITSPLSFTDRTILFSFDNNSIKAINPENGEVVSTIFVGQQSSPYFVT
                     IRDVIYIAFNEVTDKNPVAKVKAMYISTSQLWWEFVAPFPGGVSQPVAADGILFLPAG
                     NYLYAIGAGYMGSKGFDSAKTATDSDNKKNEQKSMPAPRYSEKELSQKTITPETQPKK
                     TSEQTQKQLPMRTLPVEITNDQLSPLKAKATIHYKDEDGEYKQSTAVNAKGDITVPDK
                     DNVEVIFESDGHVPKKVIVNKSDKQLNVVLDQIQPGKSVVIDNIHFDFDKWYIRKDSV
                     TILDKLVEMMKKNPGLIVEVQGHTDSTGDKTYNQKLSEKRADAVAEYIIKHGISPERI
                     SSRGYGETKPVASNETKEGRQKNRRTEFYFKS"
     gene            complement(23229..25220)
                     /gene="recJ"
                     /locus_tag="PLJ75_00475"
     CDS             complement(23229..25220)
                     /gene="recJ"
                     /locus_tag="PLJ75_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPD03726.1"
                     /translation="MYTKTAELSKKTLLHLFNITDVTADLLLLRGIDTPYKAYIFFNP
                     ELINCYSPFLFNRINEAIVLIKKCIQQKIKFGIFSDSDIDGLTSLTIVSHLFDLLGAD
                     YCYRYPVGEELYGLTIPIIDEFRKRDVQCIITLDSGTRDVEEIAYAKAHGMDVIVCDH
                     HEPSDELPDAVIINPKLHTCSYPFKELAGVGVAFKLCIALLLSYTRYYEQYVIICAVD
                     SNAYHYKVMHRGCTKECGVVDDISDIQHIINKYTNTILLHYDVAAVNALQHAKLFNLQ
                     DYSGELSNKKNLLKIEPEKVFPSALDFAVTVAQKMQFQQSKKMMKFIKDVVPLVTMGT
                     IADMMPLVDENRTLVTAGISFFNKTYHTGINVIREHIAKPINADVIGWDISPLLNTPG
                     RFGKTELAAKFFLMDKSSEVKGILDEIIQLNAERKNIIQTLVNKFSESYSANANKKII
                     MIQSNEIPEGLTGIIANRIADQTLLPVIVIANKTDSTVVKGSGRVGNHLDFFSIVSQF
                     KHIFEKLGGHEQAFGFSIHKKNIEVLEKLLHDYLQQYDTCNNNIHYDYELPIDYISFD
                     FLDELKLFEPYGAGHKPFTFISRNCSIASYYIINAKHIKCIVDGTIKLEAMAWNGKDK
                     YEAIVAQKKPVDIIYTVKTNEFNGLISPLLIIIDMHLSN"
     gene            complement(25274..25948)
                     /locus_tag="PLJ75_00480"
     CDS             complement(25274..25948)
                     /locus_tag="PLJ75_00480"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPD03727.1"
                     /translation="MLLSIDRVLQLLAEGKSIEKIAELANVDSSDVLAIIEEARKIVN
                     QFDKQKSKSKIIIKKKNNINTQIPMEMKETDEELFDGFEVLSTIPTESVLTMYVDGAS
                     KGNPGPAGIGIVIYNNDDQFVGKVSSYIGKKTNNQAEYIALIRAMLIAIYFKTKTLKI
                     RSDSELVVRQLLGEYQVNNSQIKKLYDKVMELKKQIPNFKIEHVMRNFNERADFLAKK
                     AADKKI"
     gene            complement(26221..26919)
                     /locus_tag="PLJ75_00485"
     CDS             complement(26221..26919)
                     /locus_tag="PLJ75_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03728.1"
                     /translation="MGNEIYVMLELQAIWNKVLDSKTEIARSRHAIDFWRDEMKSIGA
                     RMSDLRSRVDQLKLKIKEKEMELHDVESRIELLDSRKNSLKSQREFESMQTELDTLAK
                     KKDTIEEQLLQYMEDVEQIEGELEIFHVEMPKRLQQIENDVQQLQGKLEVMQKQAEDN
                     AKEFNKRISELPPDLRMRFAKVIDTKGNAIVPLEDGICMGCHFQVPVYVKDEVIKSRP
                     ATCTNCGRFLYLKN"
     gene            complement(26909..27727)
                     /locus_tag="PLJ75_00490"
     CDS             complement(26909..27727)
                     /locus_tag="PLJ75_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPD03729.1"
                     /translation="MNIPHKQLFDFLDNEFPLMYQEEYDNSGRQIAFDESNVTGILIA
                     MDISPQIIDEAIEKKCNCIITHHPMFFRSLKNITISSVHGFMTIRLIQNTISVYAVHT
                     NLDALCWDILAKTVRLNDIKMLFPDKRFNGIGFGAIGCMQDEMLLSQFIEQLQHELKT
                     MAIIYYGNLDKKIKTIGAFNGSGSKKINAIIQSYPVDCIVTGDVGYHDAVYANNHDVA
                     VIDIGHYSSEKPLLHFLHSKLYNYLTNAMHCSDVRLIISEKEQNPMHCGVQNGK"
     gene            complement(27711..28214)
                     /locus_tag="PLJ75_00495"
     CDS             complement(27711..28214)
                     /locus_tag="PLJ75_00495"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HPD03730.1"
                     /translation="MVNNNYKEEVIHEVEQAFSVIQAAQCGDILVSGSSNIGYALPYP
                     INQTDFALFPGGIITHQDSVFKIKDAACNVEHPLADMMQKIKGNYRTAISIKNSNAIR
                     AIAHAMDVPVIQVTAPDELMNTGFDKSQIMIVVDDDPLLYIAGQNPLDVAQIVVSIKR
                     RLHEYTS"
     gene            complement(28224..29006)
                     /gene="thiD"
                     /locus_tag="PLJ75_00500"
                     /pseudo
     CDS             complement(28224..29006)
                     /gene="thiD"
                     /locus_tag="PLJ75_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
     gene            29393..29740
                     /locus_tag="PLJ75_00505"
     CDS             29393..29740
                     /locus_tag="PLJ75_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03731.1"
                     /translation="MNYNEIFKLTADTRWNVVAQDENLWRVGIYHPEYNSAHEIDRLE
                     KHSCPELFICVNGQCGLLLREGNWEHQLIMNPLEAIMVTSYHNGFKVDKDGFFIVAER
                     TDFTTNFIERDKP"
     gene            29737..30576
                     /gene="aroE"
                     /locus_tag="PLJ75_00510"
     CDS             29737..30576
                     /gene="aroE"
                     /locus_tag="PLJ75_00510"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPD03732.1"
                     /translation="MKPDANTTVYCIIGNPVRHSLSPVMHNVAFEHLHINAIYVAFQP
                     QSAQKAIEAMKTLGIQGASVTIPFKVELASLCDEVDDLALEIGSVNTLINVNGNIKGY
                     NTDGNGLIKALEHHAVDYVHKNVLIIGNGGSARAIAYTLAHNNAHIIICGRNENNVMH
                     LVHDLQHHGISVNSCLLHDLHPDTTLPFDIIINTTPVGMSPHADTSPINEHCIHPHHI
                     IVDIIYSPRETMLIKKARNKGCKTITGEYMLLYQACMQFELWTGKPAPFEIMYNALTQ
                     ALQ"
     gene            30579..31865
                     /locus_tag="PLJ75_00515"
     CDS             30579..31865
                     /locus_tag="PLJ75_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD03733.1"
                     /translation="MLPPFLQSLVNNESVHSARYVHYSPDAIVTLLSLLHNPHTAIKT
                     IHIAGTNGKGSTAYMIAAMLQAAGYRVGLYTSPHLLRYNERITVNGNEIADETIAHFS
                     TIIEQLCTKHNLHITFFDAFTAMAFLYFQNSVDIAVIETGLGGRLDSTNVVTPLVSVI
                     TPIAYDHTAILGNTLQQIAYQKAGIIKQNTPVVIAHQHPEALATLRHEAALNNAPIIL
                     FNTNIHCSITSNHPVLTCDVVCNHPLFGGLQKCSNVTIPLAGSFQAYNAAVAIAAVLL
                     LKNYYWAIVTDDIIKALSTITIPGRSQLFGEDPLILFDCAHNPEAMQTTVEALINTYS
                     GYDCIFCVSFMNDKDIHSLLTIIQRYTQHPVYYLPLNDSRSYYPDDEVIKNYHLIITD
                     DIHLPVTIAKKSPGKTMVLFTGTFRLYPLVQTIHNA"
     gene            32123..32938
                     /locus_tag="PLJ75_00520"
     CDS             32123..32938
                     /locus_tag="PLJ75_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPD03734.1"
                     /translation="MDLSELLPKEHLISIKSTEKQLVIKELIHKLQELGKLDNADRYY
                     TQVIHRETLENTGVGNGFAIPHVRTDSVKKLLTVFGICNEPIEYESFDKQPVKYVMLS
                     IFPTSLSTKYLYLVGMMARIFSSSEKRSKIDEARTPSKIYPILTKEAKLYFESLTEIN
                     KEDEHIENLSGIPSTDLDLLIRLDQLYRLYDTGDTSEALTRKINELRKFIDNRSLSYY
                     ERMRKKCQNPFAILEKNACGGCHMVIPPVEMAKIKSKKHLAVCTYCGRFLITV"
     gene            complement(32967..34223)
                     /gene="cls"
                     /locus_tag="PLJ75_00525"
     CDS             complement(32967..34223)
                     /gene="cls"
                     /locus_tag="PLJ75_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPD03735.1"
                     /translation="MKKSQKKTHGRLNHRFKGSGHRISYSDVIHKIKNRFFLPKFLKS
                     IKKYGISGFSGGNKVTLINNGDLFFDELFAAIDSATLSINLETYIFNSDDIGWKVARK
                     LAAKAKKGVDVNIIYDAVGCINTSGELFEYLRKNKVELIEFHPIIPWRKYFNLYLRDH
                     RKLMVVDGSTAFVGGMNIGNEYAGEKYGGENWRDTHLKIEGPAVRDLEYFFLENWHRQ
                     GGALVDIARYFPVINHKGDTIVLVLSSYSRKNVKPIYQTYLAAIANAQKYIYIANAYF
                     VPDSKISRALIHAAKRGVDVRILLPSVSDIPFVQYASRYLYKRFLKNHIRIYEYIPTV
                     LHAKTAVIDDIWSTVGSTNLDRRSFNANLEVNAIVLDQNFGNKMKKQFLKDIKQSKEI
                     VFEQYQRRSSTQLIKEWFCYRFRYLL"
     gene            complement(34241..36706)
                     /locus_tag="PLJ75_00530"
     CDS             complement(34241..36706)
                     /locus_tag="PLJ75_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPD03736.1"
                     /translation="MFDYTKRSRKVLEVLAQAEGRRLNSDALGPEHIMLALLKDDDSV
                     AARILKNMGVNFEILRRNIEQSIRRSGSTIILGNLPLSARFTRIIEIAKDEARKLKNN
                     YVGTEHLLLSIFRDGTCSGIDTLIRAGIDYNVLKSEVQKILGVTPDGSTQQKGNDKTK
                     TPTLDEFAIELTRLAAEDKLDPVIGRDTEIERVIRILSRKTKNNPVLIGEAGVGKTAI
                     VEGLSQRIIHHDVPEPLFNKRVLSLDLASVVAGTKYRGEFEERLKRIMKEIKGSDNVI
                     LFIDELHTIIGAGAAEGAIDAANILKPALARGELQCIGATTLNEYRIYIEKDAALERR
                     FQTVMVDEPTADEAVEILFGLKERYEIHHKVRYTDEAIRKAVLYANRYIHDRYLPDKA
                     IDVIDEAGSKARLENCERPVDIELLEQEIEELNDRKNNLVKAQEYEQAASIRDTIIER
                     KALLSQKISDWQQRINDYAVVVDEKQIATVVAQWTKIPVEKLEESESQKLLQMEQELH
                     KRIIGQDNAINVISRAIRRSRTGLRSAQRPIGSFIFLGPTGVGKTELAKALAEFLFDD
                     DDALIRFDMSEFMEKHSVSRLIGAPPGYVGYEEGGQLTEKVKRRPYSVILFDEIEKAH
                     PDVFNILLQVLEEGELTDNFGSVTSFVDTVIIMTSNAGNREFQKIGKMGFNEINANQI
                     QFQNVDEEVKRLFSPEFLNRIDAVVHFHKLNEHHIRQILDLLLQEVNEHLTEQGIELL
                     VSHKVKNYLVSKGFNDKYGARYLRRAVQNEIEDRLAVEILNGKFKGCHKVYAGIKNQT
                     IYFKPEKNGTVGDINTPEVVHYK"
     gene            complement(36713..37762)
                     /locus_tag="PLJ75_00535"
     CDS             complement(36713..37762)
                     /locus_tag="PLJ75_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03737.1"
                     /translation="MFEQLLEQSPFWARIGPLADVAMTTRVRFARNLPSLPFATKMRE
                     SDITILETIVRQAVIASTLFQNTQFVNLRDCSSDDRRFLCERDIITHEMELSDYSSVV
                     FDHQQNFSILINEEDHIRIQVIKPGLQMLEAFKLADEIDDELNKYISYAFNDDIGFLT
                     TCPSNVGTGLRVSAMLHLPMLTATKNMGEVIKIVREYQAQIKGIVHDATKTVGSIYIV
                     SNKATLGKSEIDIIEEVDKVISIIIDLENNAREEYYQHHKDMLEDMIWRSYAIVAFSR
                     TMNYAEAIEHLSLIRLGVVLSVLKNIELSAINDLMIHIQVSHLQKIAGKIFATSEEGD
                     SFRAAYLRKRFGDKE"
     gene            complement(37762..38307)
                     /locus_tag="PLJ75_00540"
     CDS             complement(37762..38307)
                     /locus_tag="PLJ75_00540"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPD03738.1"
                     /translation="MICERCKLREATVHLTEIIRNEKTEVHLCDNCARDMGLSSHGGG
                     FSLTVSDMMSFLDVKDMNDMNDMVDSNTCATCGWTLIDYKRTGKLGCADCYSSFKEAL
                     TPILSGYHGSTFHIGKMPLKYIEHRKSKVMLDSMRLKNVATLSLHDLENMLEVAVENE
                     NYEEAAKLRDEIIKLKNQQGA"
     gene            38525..39745
                     /locus_tag="PLJ75_00545"
     CDS             38525..39745
                     /locus_tag="PLJ75_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPD03739.1"
                     /translation="MAVSKKLQTLIEQDNQYLFQNYGTRLPACFIKGKGSELFDQDGK
                     RYIDFFAGIAVNSLGYNHPALNSALHKQIDAIMHSSNWFFNKQQIEAAQLLSETSFPG
                     KTLFTNSGTEANEAAIKLARKYGISQSNDKYHIISFTNSFHGRTFGAMSATAQEKIRS
                     GFGPIVPGFIYVPFNDVTQFKKTLEANKGKICAVITELVQGEGGITVADSYFIKEISS
                     LCKENNILLIIDEVQTGIARTGKMFAYQHYDIQPDIISLAKGLGGGVPIGAIHAQNIL
                     VEFFPKGTHGTTFGGNHLAAAAAKTVLEHCKKESLLNNVNTMGKLIVEKLSLMKKKYP
                     TLIIDVRGIGLHIGIELSMAGQPIVVEALKKGLIINCTAEKVIRIMPPLTINKKLVTE
                     GLSILDSIFAGVTA"
     gene            39742..40689
                     /gene="argF"
                     /locus_tag="PLJ75_00550"
     CDS             39742..40689
                     /gene="argF"
                     /locus_tag="PLJ75_00550"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007961601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPD03740.1"
                     /translation="MIGKIPKVKTKDLLSITDFSKEEIIAIFEYTQKMKDELNHGKTH
                     NFLEGKTLGMIFEKTSTRTRVSFEVAMFQLGGYALFLSRDDIQLGRGETVGDTARVLS
                     RYLDGIMIRCYAHATALELAENATIPVINGLTDMFHPCQALADFYTIYEHNKNFKNEK
                     LAFVGDGNNVAQSLMLTAAMLGVNFALACPKGYYPAKNVVEMAFSIASKSGSVITLTS
                     DIALAVEQATYLYTDVWVSMGQEKEASRRRKNFAKYKITMKILDKCAPNCKVMHCLPA
                     HRGEEITDEVIDSPQSIVLDQAENRLHVQKSILCALMAK"
     gene            complement(40698..41021)
                     /locus_tag="PLJ75_00555"
     CDS             complement(40698..41021)
                     /locus_tag="PLJ75_00555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPD03741.1"
                     /translation="MAQKVVSQLRTLDIYARYGGEEFIICLPETMPDDAVIVAERIKN
                     IVEECTFNDNDHLITVTISSGIASLADIDEELEMIIKHADEALYQAKQSGRNRVAVYR
                     KNGYN"
     gene            41321..42526
                     /locus_tag="PLJ75_00560"
     CDS             41321..42526
                     /locus_tag="PLJ75_00560"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPD03742.1"
                     /translation="MSIKKVVLAYSGGLDTSVIVKWLLETYKCEVICFAADVGQEEEL
                     NGLEQKAINTGASKCYIEDLKEEFVRDFIFPCIKANAIYENRYLLGTSIARPVIAKKQ
                     VQIALQEGADAVCHGATGKGNDQVRFELTFKALAPQLKIIAPWREWNLTSRSSLFDYA
                     AKHNIPVPVTKDKPYSMDRNLLHISYEGGILEDPWREPDESMFLLTQSPQKAPDKPTY
                     IEIDFEHGTPAGIDGKRLDPVTLMKTLNKIAGENGVGRIDIVENRLVGIKSRGVYETP
                     GGTILHVAHRDLEAITLDRDTQHLKDRLAIEYAELVYNGLWFTKRREALDAFINESQK
                     NVTGTVKVKLFKGSCMAVGRKSPVTLYEPDIATFDDSALYDHRDATGFINLFGLQTKI
                     EALLNLKRK"
     gene            42528..43463
                     /locus_tag="PLJ75_00565"
     CDS             42528..43463
                     /locus_tag="PLJ75_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03743.1"
                     /translation="MGDSSLNMVESTYVDRVRNHSLFKESWIAFASDYPPSHCLLDFN
                     QLPSTDVALHGGDLGFALLWIVQSVLMRCVYFDINQLQPWEIPALTPVTIGAMAHTEP
                     DTARMTARYIDNNTLSLSGHKKYITGGKDADLLFVTARRTGEDKFTKIVYIQTYQLPE
                     NALQQLDMSILPTINHATLTLDDFPCNALQCSPVKDKELRKILKKWGMVERALIGEAY
                     IGYLCYLAKNITMPDQNELIDEVENLLALQKTFTRQQLENAFSNNYIDTLGDVARVLS
                     VTQKISNYCMENPQTTGDSITLRLHDLNLFNKIRL"
     gene            43585..44493
                     /locus_tag="PLJ75_00570"
     CDS             43585..44493
                     /locus_tag="PLJ75_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPD03744.1"
                     /translation="MFTSVENTIHELEKQKYICSKDIATTVYLALKMEKPVLVEGPAG
                     VGKTDLGKCIAKSLGFELIRLQCYEGLDEAKALYEWEYAKQLLYTQILKDKLSRILED
                     QATLKDAVNKLTSEEDVFFSEKFLVARPILKAITNDITSLLLIDEIDKSDSEFEAFLL
                     EVLSDYQVTIPEIGTIVAKNKPFVLLTSNNAREMSDALKRRCLHLYIDYPTKELEERI
                     IQLKVPECGNYLIKEIINAIHEIRKFPLKKDPSISETLDWAKALTLMGCEVLNRDIAI
                     DTLNFILKYEKDIELVKKNLHSIIVH"
     gene            44500..45876
                     /locus_tag="PLJ75_00575"
     CDS             44500..45876
                     /locus_tag="PLJ75_00575"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD03745.1"
                     /translation="MFHNNNIIKNIIIEEDKAKGVFFEFAEVLKKAGVDLSIVEIMDM
                     MNAISHISILEKEVFKQTLATTLIKDYTDIPVFNQCFEQFFERKKGNDSLLDIYTLHA
                     HSEDFIFTPEETSLLNSLIDNFLESHPQSFFKKSNEYLTKIFIDEELSSESGGAVGLS
                     LFNKRSQFASAGANDSDYQELSPQLFEVIMGMIRKRMFERTMGSTIKQREDYLLNKYI
                     YQLKPDEIKEMRELIKRFGQKMKNRISLRKKRVKRGNFDIKKTLRRNLQYGGVPFKIF
                     HRDRKIDRPQLVVLCDISSSVNQYSRFMLLLTYTLQSLFAKVRSFAFISNLVEITDLF
                     REMDPERALNSIFSDTNFTYGWGSNYGNSFNQFIQNYSDALTSKTTVLVLGDARNNNQ
                     DPGLDSFIKMKERSRNIYWLNPDKQHLWNWSDSIANVYIPYCSEMREVQNFLDLSHFI
                     DTLFIQKR"
     gene            45996..47066
                     /locus_tag="PLJ75_00580"
     CDS             45996..47066
                     /locus_tag="PLJ75_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD03746.1"
                     /translation="MENKVHLLIIDDEDIILKSCQRILRKREDLTIDTATNGREGLEK
                     AKAKKYDIVITDLKMPVMDGMEVLKTLRKEQPDVTVIIFTGFATVDTTREALKLGAFD
                     YIPKPFTPDELSNVVDNAIKARQNKAESAMLDLLAIVSHELKSPVSVVHTTAETLYKG
                     YFGNLSPDQQKIIETILRNCQYLEDIIRNYLDLSKMELDSLESFSQQIDLYEEVIKPI
                     VEMPENTNNIKNMKLELNIQVKPKIMGDANLLKIVVTNLINNAIKYGQANTTINIELT
                     QQNNEYVFSVYNEGVGIPEEDMEKLFKKFSRLKQKGTEGIKGSGLGLYICKSIIEKHK
                     GKIWVESEYGKWVKFSFSLPLS"
     gene            47148..47471
                     /locus_tag="PLJ75_00585"
     CDS             47148..47471
                     /locus_tag="PLJ75_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD03747.1"
                     /translation="MEIELKDIGEHKVISVSGEVDLYNVSELKKALFSVTDGTYSSVV
                     VDLKDVNYMDSSGIGALVAGQKKMRAHNGKFALINIHDDVLNILKLATLDRFFSIYED
                     ENDLI"
     gene            complement(47496..49100)
                     /locus_tag="PLJ75_00590"
     CDS             complement(47496..49100)
                     /locus_tag="PLJ75_00590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPD03748.1"
                     /translation="MSKEKASDNEKNKKTSKNKKKNIKDDNSNTIATSKKEVRFSLRL
                     KFSLAIILLVSSIITVMTYYFIQQESELLRKQILQFANREVERLAVIARESISQKDEL
                     PLVGAVQDLKNLEFIDYVYVIDTNKRIIQNFDPQMNGTIIDDDLTSKASNNTDSGKPL
                     VVVYRDNKKNVNFYDLSLPLFHTVQTVRIGTVRLGFSEEIILNEIRKLTITILLIGSF
                     FIVISIVGSIVLASVIIKPIKKLTEGAVIIGGGNLDHKIEIESSDELGKLANEFNQMT
                     QRLKVAQQVEIESRIMQEQIDLAREIQEGLNPMGFYNKSGIEIKGFTRAAKGVGGDYF
                     DYIDIDEYRVGALISDVSGKGVPASLVMVMIRTVFVSYIRRGDVQCADVVRAINDSLS
                     ADFAIDKFATLFFMIYNRSNNELSFSNAGHGPLFCYRSSLDACTLTKLEGVPIGIMED
                     VDYKQAKVLLNIGDIIIMYTDGVTEMRNADKEEYGQKRLKDLVVANKQLNAKELSDLI
                     VKDVDNFRGDVPQHDDMTLLVLKRTQ"
     gene            complement(49104..51053)
                     /locus_tag="PLJ75_00595"
     CDS             complement(49104..51053)
                     /locus_tag="PLJ75_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03749.1"
                     /translation="MINIFNIQVKKEKIIIAFIVIVISFLTATSIKPQVQNKQLIDNY
                     NKQAIEYYNKKDLTKAIIEWLKILEIDPNNEDAQRNIELVYEEKHKKDISFQKAKLYY
                     RLSRKVLPEDIDAAKNNSSIAIEHFIVAYRLDPNDPELQILKDELKLLSDEIKLELAK
                     KRLSEEIKKKYYALLSEAQILMNEKRYEEAIKVWDEMLRLVPLDAVAVEGKRQAELAI
                     NNRLKFEKIMALIATGEEFFKQEKYIDSRNEFVQVLKLDEKNRVAKDYIAKIEDILDE
                     KRTYEQRRLQAEQFYIAGVNNLKTYEFTQAQDNLENALALIDNYKDARALLESISQLK
                     KEWEERQRLLRLQQIDKTFQEGLISLTEGRYRDAVSAFQATLTLDPKNELARRYLSTA
                     LDALRVEEEEVINENNPYYSIVQPLIESGKRLYSQGNIAESREQWVKILNLFPQNRIA
                     TEYLLRCDLAKNPESFKKFAETIVNDGKKLLADKKYNEAFKKFDLIATIDPQYPEIQN
                     LITQSKVEKRKTGGEVADIGFRQRYNMALQLYQQGGKNNLEKALAEFKIINNKYPDNI
                     QVAIIINKIESQLRFQVAEQVQGKKLTPRQQELIREYYFKGINYYTNNQFDLAITEWR
                     KVLAIDPDHEKAKNNIKKCLILLGR"
     gene            complement(51060..51506)
                     /locus_tag="PLJ75_00600"
     CDS             complement(51060..51506)
                     /locus_tag="PLJ75_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03750.1"
                     /translation="MITKKMFGVSLCMLVSFMLFSCSNQRDYSDIENLLNRWANSVRY
                     LNYAEYTKLEAYPKDSNTFKTMYQNYYITNLQIVEVDDKLHNGTDSDGKPYEFKNVKF
                     IATIINRQSGKGEQVVNGDIKLIRYATGTKKEKGWLLSNRTLIHQQ"
     gene            complement(51555..52136)
                     /gene="amrA"
                     /locus_tag="PLJ75_00605"
     CDS             complement(51555..52136)
                     /gene="amrA"
                     /locus_tag="PLJ75_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPD03751.1"
                     /translation="MIVLNDEQKIKLLTLARKTIEEYLNKRTLPKVDFNNEFNDEVFK
                     QKCGAFVTLHKNGALRGCIGYVKGIKTIPEAVVDMAKASAFEDPRFEPLTKSELDKID
                     IEISILTPIEPVNDISEIVIGRDGLIISSGFRSGLLLPQVATEYGWDVKTFLEHTCYK
                     AGLPPDAWKWNNTKIEKFSAIVFSEKELKLRYC"
     gene            complement(52216..53034)
                     /gene="amrB"
                     /locus_tag="PLJ75_00610"
     CDS             complement(52216..53034)
                     /gene="amrB"
                     /locus_tag="PLJ75_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPD03752.1"
                     /translation="MWIRQPGFAGSFYPANPDKLKNDIQRYIANAKIVSLDKPVAFIS
                     PHAGYIYSGPVAAYSYAQLKHKPVDVVIILAPSHRVRFNGASVISSGIYQTPLGEVQI
                     DETIAQKLLQNNVFEEIKEVHQYEHSLEVQLPFLQVVLPQFVLVPIIIGTIDFTTCKE
                     IGSEVAKILHDEQRRFVIILSTDLSHYYHYDIAKKIDSHFIESLRKFDELYLYEALRS
                     DKAQACGEGPVLAGMIASKQLGATKVEILHYANSGDTAGPKNEVVGYLSAAFVQ"
     gene            complement(53271..55202)
                     /locus_tag="PLJ75_00615"
     CDS             complement(53271..55202)
                     /locus_tag="PLJ75_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPD03753.1"
                     /translation="MKRLFIFIVAIVLSGTASVQADDEAVQLEDVIVSDTLLYPGAYQ
                     SIKGNTVKDSSNNDLISVIKNQIPSFYTGANRVLGYGIARSGAATMSMRGIGKSGWGP
                     TTGIPILYNGMDTTMGIMGHPVADIMNMKNVDSIEVLIGPQPVLFGSGAMGGVINVIT
                     KRQKEDGYTTEIQGSYGTWNTTEDYLYHMGKFGKLDYSAGYQFQKTDGDWEQTINGKT
                     FTSEFMQQNGTVHVGYALSHNWYAGVDSYGMKQNIHDPGPEGIDALSEIPLTNQLNLL
                     EEFDITRGGVVVKLAHDYGNYSGTLQAEGNFGNHESKQSNTDIDKFESDDSSYAVRLK
                     EIVKLWQGNTVTTGAEWRRYGGTAKDIENDSYYMKDKFMNDTSIYVLMDQNFLKNILS
                     VNGGTRYSYNSEYGDYIAWQGGAAVRPFKKTKFYSSVARGFKFPDIRQVYLKGMYPAI
                     NPNPDLKPETYTMAEIGMEQKLGDTITIHMGGYRIWSDNMFVYTSQWENADEFSYNGA
                     EAMITVQALKWLSFNAGYSYIDNKQENGYLPYVPAHKANGRITLTIADFTIQLDGEYV
                     NELYADTQGLKEFDSYFIINSKMSYTFLQKYTAFISLYNITDKEYSTFAVLVPPLGDS
                     YCEYPMPDFHFLAGLQATF"
     gene            complement(55460..55873)
                     /locus_tag="PLJ75_00620"
     CDS             complement(55460..55873)
                     /locus_tag="PLJ75_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPD03754.1"
                     /translation="MSNSRITISSLACCISLIFFWAIITLHGCTVSHNTNNSITIVIV
                     SAKNCESCSMTDDTIQYVQTHYPDSIIKKVDINNPDGAMYVSTYHLWRVPVYLFLDNK
                     GSELYRLEGEQKRSDLDTAINIVSKQYEHVNILKK"
     gene            complement(55863..56966)
                     /locus_tag="PLJ75_00625"
     CDS             complement(55863..56966)
                     /locus_tag="PLJ75_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03755.1"
                     /translation="MKVKLTHISLIIIVIIAGIMLYITHYANKNDTIAEEAAGESSHG
                     YSTVLEEMGLISPQQALQDYKQLSIYPPNSRPLSKENIDLLMPNRRYEQPLPIEEGSD
                     IYYLFTADKYRVVGDDTITFVLIVLRGTKPDSERIPIAITKSVIKKGLNKNKAKYYAE
                     LSLAKNTTGDYTAELTCSQSFSSEKKSSAYWAEVEFNAGNEHVEAAIPFEYFPENTIP
                     AKFTGSFKERIADGSLYIDVEIDVLRKGYYIVDANLFDAEGEPVAYTVQKQELQSGKQ
                     MVPLLFFGKVLLDKKAKPPLTLKDLRGYRFIEATTPDPEFMDREMMKPYDGEYKTRLH
                     SLQGLSDKEFEDAMKRKRIEFLEKENTGDIGVQ"
     gene            complement(57277..58005)
                     /locus_tag="PLJ75_00630"
     CDS             complement(57277..58005)
                     /locus_tag="PLJ75_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03756.1"
                     /translation="MKRFAFLILLIGFMFVASAGFSTTYVLHLNGMCSTHWLDGKSKG
                     GESRLANAPGYVSVNCYVDNNNTIAYSASQFKTNYLDVYCKGSNWCYVVNYSAGDAIV
                     GYINANYTPQWNIAYVYTTAGAGGGSEISIRGISDLFTCNLGGQLGVSTIRNMYNHND
                     TNGIIIYHIGGYKGIFGASALLPGEDDGAVAYHSSAACVNSGSYNNLCNCTHWTNHVI
                     AYTCSGYYLNHYEMKMKFITQLGW"
     gene            58497..60011
                     /gene="amrB"
                     /locus_tag="PLJ75_00635"
     CDS             58497..60011
                     /gene="amrB"
                     /locus_tag="PLJ75_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPD03757.1"
                     /translation="MLKKIITILLIPFLLQCTSKSYSITKQSKLAGTWYPDNAQQLSQ
                     LISSLTRNAKHHAINRTPMIIVPHAGYTYSAYVASKAYGAIVTYKPDIIIIIGPSHYE
                     QFTGFALPVYDAIATPLGTIPVDKKTVQKLSANNNFVFNNKAFEPEHSVEIQLPFIQY
                     LFPGIPVIPILTGSTTPDTAKEAGCHLLYAIAHYKKPLFIISSDFTHHGPRFFFTPCS
                     NSNKSKQMQCIKQGDQQAIDYILSCDADKLYDYCKKTGITICGVNALIFGLSIYEKVT
                     TKQLAGYATSADITGDFDNTVSYAAIVIDGMLNTQAKPYANNQPSMLSDNDKAFLLSL
                     ARQSIQSYVLSKKPFHYSGTIPEACLHNAGAFVTITIQGNLRGCIGYIEPIKPLYKTV
                     IECAASAAISDPRFEPLSPEEYSTIRIEISVLSPLKKISSLDELTPGKDGLVVVKGNR
                     RGVLLPQVAQEFNFTKEEFFQQTCNKAGVYPFEYDSIIMYTFTATVFHERTPSP"
     gene            60024..61145
                     /gene="amrS"
                     /locus_tag="PLJ75_00640"
     CDS             60024..61145
                     /gene="amrS"
                     /locus_tag="PLJ75_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPD03758.1"
                     /translation="MSRLTRKDILQAGIASLQTAFFTGTIAPLYHCFAAISYHEAMHW
                     KKVDTKTVQCMLCPNQCMLRNGDKGACKARQCINGTLYSLVYGNIAAYHIDPIEKKPL
                     YHFMPGNEALSVATAGCNFSCKFCQNWQLSQSYPHELDSKHITPVQLVNNALNKKTPI
                     IAFTYNEPTIQYEYIYETAVQAKKKHVACVIISNGYCNKNASVLLYPMLDAIKIDFKA
                     YSQKFYLEICGGNLQPVLNNIILAKKKNVWIELVNLVIPTLNDNPNDIKAMARFIKSH
                     VGDTTPLHFTRFYPIYQLKNIPPTPVSTLERCKEIAVSEGLKYVYIGNVPGHPANNTY
                     CHACGKLVIERSGFYTTSNKLKSGHCPHCNTRIPGIWTS"
     gene            61142..63316
                     /locus_tag="PLJ75_00645"
     CDS             61142..63316
                     /locus_tag="PLJ75_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03759.1"
                     /translation="MKKKVLFVILAAFFIGFSSSIAQAIMIRELLTIFRGNELSVGII
                     MAIWLGGIFTGAVFSKTKQIVHPKFIIAMIIVHPFLFLVSLIVLYSIPIVYSSYGAFY
                     QLDAEIMIALLCLFPQCFVIGYVFPVLIQYADELLIKHPGSIVFLLESSGAFAGGIVF
                     TFFILFVLNPIGTAIVLLLCATILSLLIYNQKKHYVTITLVCIILLFLTTQTNKAEQA
                     LIQKSFAINHPGSLLLYKRTPYQMMYVAKNYDTYSLYGNNIFYTQLPDDYEVRPLLHA
                     LFASIHDILGKNILIAGDTLYLPYVATKLQASIDYCTVDRSLWNTFHTLGKQLYPNFD
                     FNKLNVIFSDTRGLLSKTPRRWDGIVIIPPPPDSILHNRLYTLEFFQFCRMHLNQNGV
                     FIMQIEGFANIMNPSLQRYISSVLNSFTKMFPTSIVSAGETIYLIGFNTGNTIDFNNY
                     RQNYLANFLKSDTGKSVAALIPHFNPKELDMFFQSTHLAYLNTAIKHSAMLNTDIMPQ
                     AYFEYLANTLKQTDSYTGNFIMQPAVMAIIMICIVVAATVYFLRRGKEHAVKGLVVFI
                     AGFISMSAMVIALMAYQNVYGILYYRIAFCNALFMLGLASGCTTTFAAKPLYAMRIVI
                     LIGSLAMLALFIIMKHVWLLYCAIFFIAAITGSLLPLLLKNTQETYTKRLLASTLDAS
                     DHLGAMCGALAAPFIVLPIFGMYSIGILLLLSISLFIFKKWI"
     gene            complement(63341..64114)
                     /locus_tag="PLJ75_00650"
     CDS             complement(63341..64114)
                     /locus_tag="PLJ75_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03760.1"
                     /translation="MIARQYLITPAAGKRMIAQSMLYIPEIIEALRSKTIVIVAGTTN
                     GYIAEEMLRHVNQSEGFSMQHFFRGITLPSWYKRTESGRLPDESTFKGDVVIKGGIYE
                     KNKTIFDVMGDLQKGDIIIKGANAVNIELQQAAVLIGHPQAGTTGAIMQVVIGKRVQL
                     YIPVGLEKRLHDNINELSQMINATTTEGVRYFPVTGNIITELDAIHLVIGAQAKLFAA
                     GGVGGAEGSYWVLVRGSKEQIQKMDEWYATVKNEPNFVI"
     gene            complement(64236..65588)
                     /locus_tag="PLJ75_00655"
     CDS             complement(64236..65588)
                     /locus_tag="PLJ75_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPD03761.1"
                     /translation="MELKRQLGLFTGIMVIIADMIGTGIFMTTGNILGMTNNALIVIL
                     LWVLGGVVALCGALSYSELAVLWPDVGGEYIYLKKIFGNLPAFLTGWVSLIVGFTAPV
                     ATSSMLLVQYLNKFFINIHATGITTITDDIFWQKNIAALIIITFGCIHIMGVKRGSAI
                     QNILTVIKIAIVAAFIIAGFYVLDSSAIHRLYAHYGTVEAGSIPLIGLSLLMVMFAYT
                     GWNGATYIAGEIVAPEKNLPRIMFWGVTITTVLYILLNIVFLMSTDGINIMNHDEIGA
                     IAANALFGPVASNFFSITIAIILLSSVSAQMMLGPRVYYAMANNNMIFSLLSKISNRF
                     GTPLYAIVLQMILAVIYVYSGTAMSLVIYMGFALNVFPVLTVIGLMVIRKKHPELKSS
                     YRTPLYPLVPLVYVVLTIIMMIAALCCWTITSAFAIGVLLLGIPVYYIWQRYTTGKSS
                     "
     gene            complement(65600..68347)
                     /locus_tag="PLJ75_00660"
     CDS             complement(65600..68347)
                     /locus_tag="PLJ75_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD03762.1"
                     /translation="MYLDSLEIFGFKSFADKIKIHFEKGITAIVGPNGCGKSNIVDAT
                     KWVLGEKQAKNIRGDKMEDIIFAGTDQRKPLSLAEVSLVINNTNRILHIDSDTVTVTR
                     RVFRDGESEYMINKSPVRLKDIEKLFLDTGLGKASYSVMEQGKIDLILSTKAEDRRYI
                     FEEAAGISRFKVQKKESLKKLEDTTVNLARLNDIIKEVEREKDLKSKQAEKTKIYFGL
                     REKYKRNDISLQLLRYRDFQKKLQKVTEDREKLFAEREELSKKVTLISSENENDEKRK
                     NDIQLQLFEWDKKLHTYTIKLEDIDGKTSRNKQLIQQQEQQITATRQRINEIDETVKK
                     LTEEKEKNIQQSQIIVNNIASDKQQLENLYTHRKKKIQNIHSFRDAIEQKKHAIKDFE
                     ITVKKLRESLEIVIKQLVDAIEKRKSELESSESQREQVREQIHSELAEAETCLQNALH
                     NLSLNLVDEAIQQLKKIKFDVLKQDIQLFENYEDGFRSILFDKTGIHAEKERLDKAIL
                     KTTEDIETLRNDINQLELSIHSEQEELDEVNNMITRVEKDLSRNENEKAWIDKHIQTI
                     ERQIADDVKQKVHLENEIKKLQHYISELVKEIEQWQNSMVEFNEKSNDLKQQIHDLIV
                     KRDEIEKKIIGRKQSSKKDMEQLQRLADRISSLDKNIVELQFRLNSIEEYLWTEYETR
                     LTDTSIKLDESLYESINSELQNIKQQIQELGPINNLAIEEYKDLKKRFEYYIEQKKDI
                     EKAREDILSVIADINKQSVDLFLQTFKEIQKNFQEIFKQLFNGGEAIIELTDPENVLE
                     SGIEIIVRPPGKKNKNINMLSGGERSMIAIALLFATYMVKASPFCFLDEIDAALDEEN
                     IGRFIRLVQKFSKSTQFVIVTHNKKTMSVCDSIFGVTMEEPGVSKVLSVKLETAYVMS
                     K"
     gene            69127..70128
                     /locus_tag="PLJ75_00665"
     CDS             69127..70128
                     /locus_tag="PLJ75_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03763.1"
                     /translation="MKNCNNIMIHIAIVMCAVMLVIACDRVNLHEIADSDSRTAYAIA
                     YNGSVYKLIIANIYFDKKFTSSTVLPNPMGGIAVNKEKDIMVYNNTTMLALSTPDLKN
                     WITSGAYPLYNTVLGYHDFFLYFNEINQQINQFDEDTMSWNVFATASSNSLGMFQANN
                     SQIYIVEPSGTNLLFYTLDNPSTPFLTVDIGFTLVNPFGGYANNNIFYIWRYNPTNSI
                     FKIYSGTYNVLNPSIAVGDILTDVTVTDDDKVFAIVSESGNVYLKQIISDGNYPSLYN
                     FGTTGTFTIDTLDSTHLIIASYGNTESYNGLFIYNIKDRKIEKIITTEDVIALCVPR"
     gene            70565..70987
                     /locus_tag="PLJ75_00670"
     CDS             70565..70987
                     /locus_tag="PLJ75_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03764.1"
                     /translation="MKTFTFSSDTVKLTKSKELIDNTATYELLFALSENAQCVELSVS
                     TQDRNGRKVRCAYIVESLVDVSKYAKKPAKPIYAEIAKNFDINWRFQSKISICNDKTD
                     PLKRLNKGNYRLRISAFTNDFYIFTVDITSPVEILFIQ"
     gene            71001..>71226
                     /locus_tag="PLJ75_00675"
     CDS             71001..>71226
                     /locus_tag="PLJ75_00675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD03765.1"
                     /translation="MPKHYDDIESEDLYEMAMQWAAQGFFEKAIELIKRSIELNPHFI
                     GAYIDLANLYAKKHQYQDAFHILKKASKIDT"
BASE COUNT        22715 a        13688 c        11759 g        23064 t
ORIGIN      
        1 taccatccct tcaggatatt cagtgataac ataaatataa tttaacaatt ccccggtaga
       61 tacaattgga acaattgaag gaatttttgc atttattatt ttattcaatg aattgtgaaa
      121 tttaataaca gaatatttat caatattctt tttatttata aaacacaccc tataaacatt
      181 ttcattttta atattaaccg cttcataata ttcaccaaaa aaaactgttt gtgtcttttt
      241 tgttattcta tacttaccat caagtagatt tttaggtgca ttaaaaagat cagaagtatt
      301 catgttatat gctttcagtc ttcaatgtta cattatattg taatgcaagg ttacttttat
      361 atttgttaat gaaatcttct ttcttctggt tttccagtat tttttttatt tcatctttaa
      421 ccttttctaa cggaatatat ttttttattt gtttaccgtt cactgtctcg gtatatgcat
      481 aatacttatc ttttgttaat tcatattctt ctttaatttc ggctaatgaa acattgacat
      541 ttttaggggt aaggtgcttt ttaaattcta tttcaagtat catatcatgc ttattattca
      601 aagattttac aaattctggt tcattatgat aacccttttc ataaaacact cttttaacca
      661 gctcattggt aaaatattcc catgcgtatt caatcttaaa tgattttttg gtcatttcat
      721 tagctatagc tgcatcactt cctaattgta tttttgattc tattaatttt tgtatccttt
      781 tattgaaatc atggaccgtt atggatttat ttccaattgt aaaaagtatg gtatttacag
      841 tattactatc cagtgcatta taattaaaat aaacatcttt cccgttacgc aggttatcta
      901 tatattcatg taacaatcta tttaaaacta tattttttaa atcatgcgct tcttttttat
      961 cgttagttat gaaatataaa ttttttaatg tagctgttct ttcatccttt ttaaagacaa
     1021 tatagatgcc actattaaac caatcattcc catcttttac aattattggt tccatgctat
     1081 aatcattttt tgctaatttt tcaatacctt tttgtaaact ttcgggtaac ataaaaagaa
     1141 atgtatatct aaagtccatg cacataaaat ctttttgatt gttactatac tgcttatata
     1201 catctaaaaa acttttactt ttacatgttg caattacatt tactgctttt ttgtatgagt
     1261 cgtctgttac atcattgata tagaaggcta aaatatgtac catcggttct tttattttag
     1321 ctttttcttc tatctctttt tttaaaaaat atttcattat ttcctgatcg gcatatagct
     1381 ctttaatatt ggaaaaataa ggctgtttat catagccctg tttttttgct tccaagatca
     1441 atatcttttc tttgcacatt tcctgcactt tttgtattcg caatgttttg tacttcatta
     1501 tatggctaac ttcacgctta taataattca gctccaacca atccataaat tcctgtcttg
     1561 ttacagcacc tccattatat gttgccagta tatcattgcc cttgttacac tgcaaagaca
     1621 tcaatagtgc aggtaacaat aaagctaaac ttattacgtt aatactattt tttctggtta
     1681 tagttatatg catatactgc aacacctcca ttccagtagt aataaaaaca tactcatacc
     1741 ttccgtgtaa gaaccatggt cacatcatca ttggtatgat aatttcttaa tgctgcaata
     1801 atatcatttt ttatctccgc aagtggtttt tcccctgaat tttgtaaaag gttagccaat
     1861 tgatcttctt caaacatttc tccgttatca gtttttgctt cagtaatacc atcagtgtaa
     1921 agaagaaaaa catctccttt attcatagta aattctttta aggaagtatc acggtttaaa
     1981 tcgatatcaa atcccagcca taatccatca gattccattc tatctacctg tttcgttgca
     2041 tatctatata caagaatata ttgatgaagg cctgaatatt caacctttcc ttcaggatac
     2101 agttttaacg cagtaatggt catgtactta tcttcattca tttgcttaat attataggta
     2161 attgttctgt taacaacctt aagcaaggta accggtgtta aatttggatt tgcgcgtata
     2221 atagtttgta tagccgtttg caccatcatc attaccaatc ctgccgaaac accatgccct
     2281 gatacatcac caatgataat ccaatcattt gtactggtat taataacatc ataatagtct
     2341 ccacctacct cagttgccgg tatcatgtaa ccataaattt catatccttt tgccataggc
     2401 gattttggta ataaaacagt ctggatttta gcagcaagtt gcatttcacc ccataaagca
     2461 tctctagcct cttttagttc ttttgttcgc tcctctacct ttttttcaag atttaagtaa
     2521 agctgagcat tttcaatgga gatagcagct tgcgaagata tcaaagttaa caatgctata
     2581 tgtcgttgtg taaatgctgc tgttattaca ttgttttcaa gatacaaaac agcaaccacc
     2641 ttaccttgat tgagtattgg taaacacaac aatgacttta ccttattttt aaaaatatgt
     2701 tcgtcattga catagaaagg atcattcatc gcatcattaa tgattacttt ttcacctgtt
     2761 cgtgccacta atcgtactat tgcatgcgaa aagccatcta ccctgtctac tggaagattt
     2821 tccagagctg ttatttcctc atctattgta tttttcctgg caacaacaat caggtcttca
     2881 ttatccttga gtattaaaaa gcctttctcg gcacctgcat tctctatcat tattgacagc
     2941 aacttccccg gaagcttttc aaaattaagt tcaccggcaa ttgccagcga agcctttaat
     3001 gccgaagaaa aatctactat atccagtgaa cttgctatag atgaactatc gctatcaatt
     3061 gctattgctg ttcgggtttg ttcaatcttt attttgtcac gatagttatt gtgtaacatg
     3121 gttgcttttg catctgcacc acattgatgg aaaaggttat atgccttctc aagctgttgt
     3181 gaagcatttg ttttatcgtt taatgatata tacatgctac caagacattc atgcgcaatt
     3241 gcctcaatga atatgtatcc atttctatta gcttctgaga ttgctttatc ataatattct
     3301 tttgcttttt tataattttt ggtaactgca tacttttccg ccagcataag ataatattta
     3361 tgcagataat taaaagggca aactgttgac cactttttca tcattgatac aagtagtttt
     3421 gatttcattg cccatattat ttttgaaatt tttttatcct tcctaagccc tgctaatagc
     3481 aataagtagt ataaaacaaa ttcaggataa tgaatattcc caaccagttc attttttatt
     3541 ggctcaatct cccgggcata ttcatatccc ttttgtagat tcctatataa aaattctatt
     3601 ttcattaaat taaacacaaa gatgaattta tcaagattgt tttttgtttg tcgtactgct
     3661 tgtaatcgtt cttcggtata attgtcatgt atattgtatg ttgatggaga atcggtaagc
     3721 cctttatagc aatagtattt acgaatatta tcctcatagt atgaatggaa aaatttatcc
     3781 ttttggtttt tgaaaaactt ttcataattt ttatattcag taaaaatttc atccaggttt
     3841 ttacccagta taaatgaata atcagcaata ttattgattg aatgtgcagc aaaatcaaac
     3901 ttacccaatc caagaaacat gttatatgat tttctgaata gtacaatatt ttcttgcaca
     3961 tgagcattcc aatgtaaata taaatatgct aaagtaaaat atacgctgca tgcatttttt
     4021 tcaggtgttt tatctaatag cttcattgat agttggtaga attgatttgc ccatttgtaa
     4081 tcaccaagcc ccggacctac tatacctgct aacaccgaaa aaaggtatga agacaaatag
     4141 caatttcccg ttttaataat aaagttgaaa agtatgattg ggcctataat aaccagattg
     4201 cgacttgtat aatagccagc ctgtgttaca ctttcaaata ttcgtataac tgcaatttcc
     4261 tcggggtttt ctatccatgg gagctgtaat aaatcttctg gcttaataaa ttgtaatttt
     4321 atctttaaag caagcattac ctttatgaca ttcagcatgg aaggtttttc gggtatgcta
     4381 atcccaaaca ttttcaatcc ttccaatccc agccttaccg cttcttcata ctgcccacca
     4441 ttggttaaaa ataaacatcg gttaatgtat atttcggctt tatcaaggcg gctttgtgca
     4501 taatgcgata aaacatcaaa tagtgtagca gcattatttt gatcacctgc taaatattca
     4561 ctttctgcat aattaagata aatcttgaat gttagcctgt agtaatcaat ccatgcattt
     4621 tctggaagaa gttcagcagc ctgtttaaaa taaatcagtg aagaatcaaa tgcagacgaa
     4681 tttttagcct tttcaccggc tattaaattt aattcagcca attgtatacg ttcttcaaca
     4741 gtttgaatca aatgtactga tttattaaga tgataaacaa tgtaaaatat tttatcctga
     4801 atattttctt ttgctgtttt actcaatgaa tttttaccaa tcttataatg aagcttactc
     4861 cgctcctctt catccagcaa tgaatatgca gcctcttgaa ttctatcatg gtaaaatcta
     4921 taaatatttt taaataatcc aataaatcca aactcaatga gtgaattcat tacttttaag
     4981 attgcttctg cggtttcacc tgttattgca atgatatcct ccagttcaaa tctattgcca
     5041 atacatgcac aaatagttag caattcaatg ttttccgcag gcatcttgtt tatagactgt
     5101 gataacatat caacaacatt atctgttatt ggcaattcct taaccttttc aatattccat
     5161 atccatcctt tttcggggct aaatgttatt aatttatctt caaagagacg ttttaaaaat
     5221 tgtataataa aaaaaggatt gcctgctgtt ttgctataaa tcaacgaaga caattccata
     5281 gtagactcaa cattattact aagaaaattt aatagaaatg tattaacatc ttcttctttt
     5341 aaaggtgcta atgcaatgat attgatagtt acaccattat cctttatctc atgcatgact
     5401 ggattaatag gatgagaggc atcaatttca ttatcccggt aagccatgat taaaaagaaa
     5461 tttttaacgg attcatcaag aactatcgaa cgtattagat taagactggc taaatctatc
     5521 cattgcacat cgtctaaaaa taaaacaacc ggatgctcct gtacggcaaa aacatttata
     5581 aactttttaa aaagataatt gaatctattt tgcgattcct cagggcttaa ctgttctaca
     5641 ggaagctgtg gccctatgat atgttctaaa tccgggatga catctgttaa aattttaccg
     5701 cttttcccaa atgcatttaa aagctcttct ttccataatt ccagctttga taaatcttct
     5761 gttaaaatct gtcgtatcaa catctggaaa gactgaatga ttgagctata tggtatatca
     5821 cgtttaaact gatcatattt acccgaaata aaatatccct ttgttgcagt tattggctta
     5881 tagatttcat taacaagtgc agatttgcca ataccaggcg aacctttaac aataactatc
     5941 tccctgtgtc cttccataaa atttttatac gaggacaata aggattgtat ttcactgtct
     6001 cgacctacaa tttgttgagg aatattaaaa cgcaacggaa tatcaaaaga agcaatttta
     6061 aatggtgata ttgtaccttt tttagtgtat tcgtcattgc attgaatcaa atcgcccact
     6121 atgccaaacg aattttgata tcgttcttcg ggagttttag ctaacatttt atctattatt
     6181 tgcgaaacaa taccaggaat atgtgggttt acctcactga ctggcacggg cttacgagcg
     6241 atatgtgcat atattatttc aatgggatca tcagatttga atggaacatt tcccgttaat
     6301 aactcataaa aagtaatacc catggaatag atgtcagttc tataatcgat attgcggttt
     6361 aatcgacctg tctgttcagg tgacatgtag agcaaagtac cttccataat atctttcctg
     6421 tatatttcct gatattcgtt gggaaacaat gtccgtattc caaaatcagt aatttttata
     6481 gtatgtaaat cttcggttag cagtatattg gatggcttaa tatcctgatg gactatgtta
     6541 tattgatgaa aagtgccaac aatctgtgaa accttaatgg cgatggtaaa gaattcattg
     6601 agcgctacta tttttttctt cattaaaaga tctttcaagg atatgctatc aaaatcttct
     6661 acgactatgc caaagccttc atgtgtatca ataaattcat aaggttttac tattccgcta
     6721 atatctggat tcatgattat ttcgtattcc tgtttaaaac gtataaaatc tgacaatgaa
     6781 tactgtttga tgttgaatac cttcaatatt gcagtactat ccttatactc aatacggtat
     6841 gttattgttc gtggtgattc agcgatcttt tgtataattt gcattcccaa tattttttcc
     6901 attgaaatat ctccctaaca cctgataact attgatatgg aactatacat cgacattgtt
     6961 ataaatgtag acaatatata aattaagtcc agccttattt ttttgatgat gaataaattt
     7021 tgtatcatat atcataaaca tttatcatgg tgagataaac atgatgtagc gatacattat
     7081 acataatctc cctttattat gattttcaat cgatatcagt ccatgcatgt tcattattat
     7141 atgattagtg tataatgatt gtacaatttt actacattaa agtttttatt gcaggtacta
     7201 tcgcccaata tagcaaactt aaaacatatc aagttttgct tgacgtattg tcattaatgg
     7261 tactatacaa taaaccatca actataacaa ggataattta tgagctatcc tcttcatgat
     7321 atgaaaatac tggattttac ctatttgcta ccaggacctt atgggagcat gatgctggca
     7381 gatttaggag ctgatattat taaagttgaa aatccacata atcccgatat gttacgtatg
     7441 cttcccccca ttgttgatgg gatatctgcc gtttatgctc acctcaacag agggaaaaaa
     7501 tcattggcat tggatttaaa agcacctgat gctaacaata ttatctataa actcgtcaaa
     7561 gagtatgata ttgtaataga acagtttaga ccgggcgttc ttgaaaaatt agggtttggc
     7621 tatgaagatt taaaaaatat caatcaaggc ataatctact gctcgctaac aggatatggg
     7681 catacgggaa gttatgccag ccgtgcagga catgatatta actatatggc actatcagga
     7741 gttgaatcat tttccggcaa aaaatcaacc ggtccatcat tacacgggat acagattgcc
     7801 gatattgcca gtggagcaaa aaacatggtt ataggaatat tagctgcgta cattaaacga
     7861 caaaaaacag gtaacggcga ctatcttgat atatccataa ccgatggtgt ttttgccatg
     7921 agtaccttta ctaccgctgc gtatctggaa ggtgatgtat taccaatgcc gcaggctcaa
     7981 atactgaatg gtggtagcat atatgattac tatatgacgg ctgacgggcg ttttctttcc
     8041 gtgggaccta ttgaaccaaa attttttcaa agtttttgtg acgccattgg atataacgat
     8101 atggctacca ctatggattt ttcgcttgag acagtacaaa aacataagag ggatattgca
     8161 gtcattattg cgtcaaagcc tctttcatac tggattgatg ttttttcagc gcttgatgca
     8221 tgtgttgaac ccgtacaaac tcttgatgaa gcaattaaca atccaccgct atatgatcgt
     8281 gatatgattg tcaatgttac aaccatgaat ggaaaaaaac ttaaacagat agcaaatccc
     8341 attaaatttt cttcagacac atatattgca cagtatgccg gcgtttcgtt aggctacaat
     8401 aatgatgaaa ttttacacgc tataggctat aatgaaaatg agatacaaca actctatgac
     8461 aacaaaacaa taacaaaaaa gtaaggtttt agtatacaac aatgaaaacc cacaaaacct
     8521 ttaccatcat aacatttggt tgccagatga acaaaagtga ttctgaactt atggaactat
     8581 cgcttttaaa atcaggcttt cataaaagtg acagttacca tagtgatata tttatcttca
     8641 atacctgttc ggtacgcaag catgcagaag acagggcgat agctcatata accgaaacac
     8701 gcagcaaata taaagatgcc attattgcag tagcgggttg catggcacaa cgtatcgctc
     8761 atgaacttat aacagagttt ggtgtaaacc tggtggtggg accttatcaa tcaccagata
     8821 tggggaacat catctcccgc tatctttaca acagtgctat aaataagtat atttcacaaa
     8881 atgcagatga ttttgcatca cgccttgata atgaactggc acatcatagt gatgagctgc
     8941 catggcataa atgggttact atctcacatg gatgtgaaaa ttattgtgcg tattgtattg
     9001 ttccctatgt gcggggtaag cttatttcat tcccatcaca acagattatt gattactgta
     9061 cggtattaat tgacaatggc atcagggaga ttaccctttt aggacagaat gtcaatcaat
     9121 atgggcagga ctcgggcgat attccatttt acaaacttct tgataaagta gcttcactcc
     9181 gtggactata caagcttaat ttcatcacct cacatcctaa agattttaat gacaacattt
     9241 tgcatgtgat gaacaacaac cctgttattg cacgaagtat tcatcttcca ttgcaaagtg
     9301 gttcggatgc aatattgcag gcaatgaacc ggcagtacac tatgaaacag tactttacaa
     9361 ctattgatgc cattcatact attgtaccgc actgttctgt ttcaactgat ttgattgtgg
     9421 gattccccgg tgaaaccgat aaagattttg aaatgacact gaatgccgtg aaaacaatgc
     9481 aatttgatga agcctttacc tataaatatt ctccccgtga aggcaccaga gcatatttac
     9541 agcccgatac tatccctgac aatgaaaagc agaggcgtct tgacattctc attacaacag
     9601 tgcgcgaggt ttcactaaaa cagttacaca atcatattaa ctcaacaacc gatatgattg
     9661 ttgagcgaat cagcaaaaaa tcggacagtg aggtaatggg aaaaactatc tgcaaccacc
     9721 ctattatcat caagggcacc atggatgaca tcgggaaaaa atatacagta actatcgtat
     9781 cattaaaggg ttcaacgctg tatggcgagc gtactgcgta acattatcat tgtattgttg
     9841 tgcacagtaa ctgttgctgc ttcagaacaa tctgattatg aagccataca aaaagcgttt
     9901 tcaagcaacg ccgcatacgc tgatgagctt gtaaaaaaat tttttaccca ttaccctgaa
     9961 agcaagtata tacctgatat acgtatgctg gtggctgcat caaaaaaatc tgttaatgcc
    10021 agtgtagccg actaccaaaa aattattcag ctctatcgtt actataacaa acgcgatgag
    10081 gctttctttt cattgtgtca gctctactat gttaagagcg attggataaa tttgctttca
    10141 acaagcaacc gtgcaattgc tgaatgttcc accagcaact atctcccggt tttttatcag
    10201 ttcagggcga tagctcattt taattacagt aactacgaaa atggattatc cgactgtgaa
    10261 acaatagctg aacatactca tgaaataaat gctctgggat acggattatt tttaaaaaca
    10321 gaaatggaaa agaacatcac agggtattcc agaaaatata tacagggcat tacagaaatt
    10381 ttacacggct atgatagtac tgcaatttac ccttcagtgg tgttgcgatt ggggcagtac
    10441 tatgaacagc ataaaatgta taaccacgca tggtcagcct atcagtatat tatcaacaat
    10501 tatccaaaag ctcctgaagc cgtgactgcg gcgctttatg ctgaacatat aaagagatat
    10561 aatcctgtta gtgtacagtt tatgcccgat gaatcaatca ttgctgatag cacatccatt
    10621 gatatagcac cggatgtaaa tatacccgat agcaacaatc ataccgttca ctatgcaatc
    10681 aacattggac cattgtataa ccttgcaaaa gcgaaggaac ttcagaaatt gattttaaaa
    10741 attgttatgc ctgtacacat agcacgaaaa acacaatcat ttgaaatata tgctggacat
    10801 ttcagcacct ttgatgaatg catgaatgta aaaatacagc ttgctgaaga actgggtatt
    10861 aatggcttta taattgaaat ggtcaatcag gatgggaaaa actatatcta tgggcaatga
    10921 taatgacaaa gaacttacac caatgatgcg acagtatctt gctattaaac aaaactttaa
    10981 cgatgatata ctgtttttta gattgggcga tttttatgaa atgttttttg atgatgcaaa
    11041 aattgcatca aagatactgg atattgcgct cacttcgcgg cagaacgata ttcccatgtg
    11101 tggggtgcca tatcatgctg cggatagcta cattgcccgt cttataaaag caggatataa
    11161 ggttgccatt tgcgaacaaa tggaaccgcc ttcatcaagc accattgtta aacgtgaggt
    11221 tatacgagta gttacgccag gtactgttgt tgaaagcaac ctcatcactt cggatgaaaa
    11281 taatttttta ggttcgatag ttattgtcaa taatactgtg tgcgctggat ttgtggatgt
    11341 ctcaaccgga gactttttca taacaaccgc tgattatagc aatgaattta tttgcaccga
    11401 gataacccgt ttttatccga aagagatgct gctgtatgca ccacagcagc agatgaacta
    11461 tttaaaagaa ttgctgaaac agcaggatat tacattagct tctatcaatg aatggtatta
    11521 tgatactgaa tacctgacca acatcataac cgacatatat cgactaacta atatcaaagg
    11581 attaaacctt ggcaccatag agattttgac tgttggttcg cttttacatt atttaaagga
    11641 tacgcataaa agcgctctgt cccaccttaa atttccaaag aatctaagcc gcagtgattt
    11701 tatggtgctt gaccatcaga ctatcaataa tttagagata ctaaaaagtc aggatggttc
    11761 aagattgcat tcactttttg gtgtgcttaa ttacacacaa acagcaatgg gaaaacgcgc
    11821 gttagaacgt gcgctgctct atccattact tgataaaaaa gctatcgaaa gccgctatga
    11881 tattatttca tattttataa acaacataca ccttttaaaa gaaatccagg atgttctttc
    11941 atcaattcat gacatggaac gtctgctatc gcgttttact ttaaaaaaat attataaccg
    12001 cgatttcatt gctttacagc aatccatcga ggcagcaata tcaatctata ctattatcgg
    12061 gcaacaccct gatgcattgt ttaatatgct tggcagcata agcccccatg tacaggaact
    12121 atcggccacc attaaaaata ctatcgttga taatcctccc ctttcttccg agcatggcag
    12181 ggttattaaa gaaggggtta acgcagaact ggacagatta tatgcgttaa aaactgacgc
    12241 aaagaagtgg ctattgcaat accaggaaga tgaaaaaaaa cgtcttgata tctcaacact
    12301 gaaagtacgc tataataagg tcattggcta ttttattgaa gtaagcaagg ggcaaacatc
    12361 aaaagtcccc gatgattatt tcagaaagca aacgcttatc aacgccgaac gatatacaac
    12421 gcaaaccctg cagaagtttg aaacagatgt tttgcagtca actgatagca tactgaacat
    12481 tgaaaatgat atagtaaact ctttaactaa ccgtattgtt gaactgaaaa atcatattca
    12541 ggacttagcg tataacattg gaatagtaga tttgttttgt tcattggcac aggctgcata
    12601 tcacaataac tatacacgcc cagcattaaa tgacgaagga attattgcta ttataaatgg
    12661 aaggcatccg gttgtagaaa agttttacat aaaagaacca tttgtgccca atgacatttt
    12721 tctggataac accgacaatg ttattgctat tataacagga cccaacatgt cgggtaaatc
    12781 cacttatatc cgcatggcgg ccatcattca gcttatggca cagattggtt cgtttattcc
    12841 agcacaatcc ggaaattgct gcataactga ccgcattttt acccgcattg gcgcatcgga
    12901 taatatttcg cgaggtgaat caacattttt agtagagatg aatgaaaccg ctattattct
    12961 taacaacgct accgataaaa gtttgatcat tatggatgag ataggacgtg gaacaagcac
    13021 gtacgatggc atggcgattg cgtgggcggt tgtggaatat attgcaaatt atataaaagc
    13081 aaaaacacta tttgcaactc actatcatga actcacaacg attaaaaaaa agggaattgt
    13141 aaactatacc gtcatggtta aagaatccat caatgaggtg cagtttttgc ataagctgat
    13201 accgggtgct gctgataaat cgtatggcat tcatgtagct aaacttgcag gtataccaaa
    13261 aacagtaacc ataaaagctg acaaaatttt agataaatta gagaaatcat cacgggttga
    13321 attattagca aaaatgcctg ataatgaaga tcagcttgag atatttcagg caactcatca
    13381 tcgattgata caggctttaa atgaaataaa cattgaaaac cttacaccac ttgatgcact
    13441 gaatgaaatt gcacgcctta aacggcttat tgaataaacg ccagcaagct tgttttcatt
    13501 gagtcatttc atcccaccaa tcaaattcat cggggaattt tttgtagttt ttttcaaacc
    13561 aatcaaacat tttattatcg tagaatccac ctttattaaa gcgatgttca aattccgaaa
    13621 aggtcatatt catcctgatt gccagtatat aatttcgtgc gcctatgtta tcattaggat
    13681 ttgacctcaa tagtttcctt aacaaatcta atgcgctgtc tatctcacct ttatcccata
    13741 ggtcaattgc cttagtcaaa atagttctaa tgatatgccg attttctaac catccccatt
    13801 ccagtacttc aggccagttg cccttatcat caactattaa ttgtatagcg cgttcataag
    13861 ccgtattcag tatggtatca gcttgcgata tctttccttc atttaaaaga atatcataaa
    13921 gaaataaata ggtatcaaaa taatcaggat ctttttctat cagcttcttt actttagtag
    13981 ttatggattt agcattgcgt ccatcatact cttcacacag atcataataa gcatctacca
    14041 catgatggtc tttgtcccta aatcttttct tttgtttggg catagaaatc acctcccatg
    14101 aaaacagatt tatttacttc aaccacctgc ctctctgtta cgtaaagcaa ggtaagtttt
    14161 taacattgtc gttctgaggg acgtagtcct gaagaatcca tttgttcaca aaacggcgag
    14221 attcttcact ttgcccagaa tgacatacct atacatcctc atcccatatg ggtggcagca
    14281 caccggtcat gagcaattca caataactat accattacta caatttcttt ttatgtgcaa
    14341 gaaaaggttc aaccaaatca attggtattg ggaacagcgt tgtggaatta cgttctgtag
    14401 ccacctcacg caaggtctgt agataccgta attgcagggc tattggatgt ggttccataa
    14461 gcttggctgc ttctaccatt tttgatgcgg cctggaactc cccttctgcg ttgataatct
    14521 tagctcttcg ctcacgctca gcttcagcct gttttgccat agcacgctgc atctcttgag
    14581 gcagatcaac atgcttaatt tctactgttg taaccttaat accccatgga tcagtctgct
    14641 gatcaagaat cttctgcaat tcatgattaa tgcgttccct atctgataac aggtcatcca
    14701 gttcaaccat acccagaatg cttcgtaatg ttgtttgcgc cagttgtgat gtggcataca
    14761 gataatcttc cacctcggta atagctttat taggatctat caccctgaaa tataccactg
    14821 cgttgacctt gattgataca ttatccctcg taataacatc ctgcggtggg acatccattg
    14881 caactgttct aaggcttacc cgtagcattt tatcaacaaa tggtataatg ataacaagcc
    14941 ccggaccttt aggccctgtt ggtggagtta acagcctccc caatctaaag acaacggctc
    15001 tttcgtattc cctgataatc ttgattgagg caactaccag tagaaccaga agtaatataa
    15061 gggtaggtaa tgcttgaaac ataacttacc tcctgatgta gaatgttgtg taggtactat
    15121 cgctaaaact tttttataaa tcgtttgtac taaaaaaata ataggtaaat ataaaaaaac
    15181 tattatgata catcatgatg aaccttacta ctgggatcta attctttgtt ccccggatac
    15241 tttctctaca tacaatgtca acccgtcaac tcttttaaca ataatttcat cacccttctt
    15301 tactggaaca tcacaatatg cattccacaa ttcgccatga atctgaacag cacccctacc
    15361 ggtaaaatca agagttgcat atcccttttc acccagcata ccttcaattc cggtcaccgc
    15421 tttttttgta tgtaccttta ttacagcacg cacaacaata aagataaaca acagtaaaac
    15481 aatgagtaca gtaaaaaccg atgttaacgg tatctgtccc cctggtaaag gtgaatcaaa
    15541 cagtatcatt gagcccaata taaacgagat aatacccccc aatgtcagta acccataact
    15601 tgtaaatttt aattccagta taaataatac aatagctaat ataattagtg ttagtccagc
    15661 aaaatttatg ggcaacactt taactgctaa taaaaataaa aagaatgcaa cagctccaat
    15721 ggcaccagga actatcatac cagggttttt taattcaaat ccaataccaa ccacagcaat
    15781 aataaacagg aaaaaaacca tctggggatc ggcaaagtaa ttaaggactc tttccttcca
    15841 gttcatggta tattgaacct gttgtatatt gtgtgtatgc aaccttataa atttatcttt
    15901 tatttttact gatttgttat caattttttt caaaagatca tcaatatcat tagctacaat
    15961 atctatcact cccagccgta acgcttcatt attggtactg gaaattgctt cccgtactgc
    16021 tttttctacc catgcaatat tacggttccg taattgtgct aaactcctgg cataggcaac
    16081 agtatcgtta agaacctttt tttccataat tccatcagat ttttgatcct gactgaagtt
    16141 taaaaacgga ctgactggat gcatggcacc aatttcagtg cctggcgaca tcgctgctat
    16201 gtgagcagat atcatgataa aaccacctgc cgaggcagcc tgtgctcctt taggataggt
    16261 ataaacaata acaggtatct gtgaatctaa tattgctttt ataatatcac gcatggcatt
    16321 taagagtcca cccggcgtgt ccattaccat tacaacaaat tgcatattat cctgattggc
    16381 tttttttatt gaattaacta tataatccgc tacaactgga tttaccgaac cattcagttc
    16441 aataagtgca tacttttcat ctgcaaaaac aaattgtgtc aatagcacca caccaagcaa
    16501 tgcaatgata agcgatcgta tcttcatttt taaaacctca ataatttatt gtaatagtat
    16561 acagtataca atattttatg caacagtcaa gataataaat aaaaagaatt gcaatagtat
    16621 aaccgatagt tattattgag atatcttaat tttttttata tgacaaacag taacatattg
    16681 aaaaagcttt atcaatcgta tacccacatg atgattaaca atgcgagttt acgatgacac
    16741 attatatttt aaccatggaa cattatacaa tatgaggcaa actattgtca ttattgctat
    16801 cattgcatta ataggaacga tctccctttt tgcccaaaca gttaaggact ctactcctgt
    16861 tgacaaacct gacaacacaa aaacaccaga tgttacggca aacgaacaat ctacagttac
    16921 cagtaaaagc aaaacaatca gtaatactcc agagcaagat aagaagaata cacagcaaac
    16981 tgcaacaaca aaacaaaaat ctgatataaa gcaggacaaa aatcccgaag ctgaaaagaa
    17041 aaaggttgaa tggataaaaa ataccatgcg ctatggtatt cacagtgaca ggattgatgc
    17101 tatcaagtcc gcattaacgt taaaggaaca atcaaacaaa gatgatgtga tgaaactttt
    17161 aaatgagctt atgccccagg aaaccaatcc tgaggtaaaa cgaaccgcaa ttaccattgc
    17221 aggtgaactt aaaagcacat cgtctgtttc atggatcatc aattcattaa acaaccaatc
    17281 cgaagaagtt cgcattgaag cggtatattc aattgaaaga ttaaaagcaa ctgaagctat
    17341 tcccaaattg gtgagtattg tcaaagaaca aaattttact gaaaattcaa actttacaga
    17401 agcccttatt aatacactgg gcaaattaaa ggcaactgaa ttaaaagatt ttgccattga
    17461 acgtattaaa gaaacaaaaa ctactgataa tttgcgacaa ttattagtat tgtttcttgg
    17521 tgaaatagag gacataagcg ctaaagattt tttattacag ttattaaaag ataaagatga
    17581 aaatccgacg ataaggtcat atgcagcagt ctcgctttct aagataggtg caaaggatac
    17641 agctaacgac attctggaaa tactatccga gatagatagc tatccattaa acaaaagaaa
    17701 agattattat tcattatcca tgtactgtac ggcatcatta gttaaaatgg gggacagcaa
    17761 tgctatacca agactcatgg aagcacttcg aagcgataat ccagtagtca ggcttaaggc
    17821 agttgagctt atgaaggatt taaaagacaa aagaactgtt gacattttga aatataaaat
    17881 gaactatgat ccaagtccaa aggtacaaaa agcagccaga gaagcattga aagcaatggg
    17941 tgaagaagta gatgaatcaa aatagcttac atgttaagac cgtactatcg caatcaatga
    18001 cactatgtgt ggcaatactc attagcttaa tactgataat attaatctca aacatactta
    18061 ttttcccaat aatctatttt tcaacatcta atccaaaatc atttaccctg actgtagaaa
    18121 ttgtattgta tagcatatta atagctttgc ttttatatag tattatttat agattaataa
    18181 tacaaaaaaa agctggtatt tcttttgatg tgactatcaa acatttttta tttactgctg
    18241 tacaatacat agctctattt tttatagtta ctactataat tattgttttg atagcaatta
    18301 tatattattt acttaaaaca aattatatat tgctttataa actaatacat taaaacaata
    18361 ctacgatgcc cctagtaagc gattttgata aatatttttt acaccttccc agaatatcac
    18421 ggccggatga ttttgatgac ttctggcata ctgctattaa tgaattaaaa aaaacaccca
    18481 ttgatgttca atttatccac aataaaagaa aatccaccgg gaagtttact gcctacgaca
    18541 tgacctacat tggatataac aaggtgcaac tttttgcatc attgcatatt cctgataaaa
    18601 tcaacaagcc tcatgtcgtt atcctttttc atgactatga tgatgtaaca tcacatgctc
    18661 aattccccat tgatctgccg tttgcatact gttttgtaga actacgaagc catagaaata
    18721 ttattcacta cgaagaggtt gaaaaaaaat caccgggatt tatggttgaa gggattttag
    18781 aaaaaaatga ctattatgta aaaggtttat atctggacgg cttaaggact attgatgcct
    18841 tacgattaca taatgatctt gactgcagca ggatagcaat tgttggcaag gggcttggcg
    18901 cggcagtttc cgtttttacg gcttcacatt caaacagggt tgttgcaatg gtgctcgata
    18961 caccctcgtt ttgttatatt gacatggctc agaacattgc aaaaagtacc atagcaaacg
    19021 agatcaatga gtttattgag gaacataaaa ataaaaaatc tgttataaaa aaaacgttag
    19081 cctactttga tgctttacaa tttaccaata aaatttcatg tccagtatta gcaacagtgg
    19141 gttttaaaga tacgctttca cctgctgagt gtgtttttgc tctttttaat catttgcaat
    19201 gtgacaaaac aattgaggta tatcccgaag aagggcacat tccaggtggc atagaacaga
    19261 tgaaccgaat gattgaatgg ataaaaaatc agtttgagaa ataagatatt ctatttttta
    19321 attccaatca ttgataatag atttttcatc tcatctattt taaagagcaa tgacaacgca
    19381 aaatatatca taccatacaa cgacaataca atgaaggcga tagttattgg atgatagtga
    19441 agcacacttt tgatgagata ggcaactccc gctgcgatga gagcacttac ccatagcgac
    19501 actatatatc gtttcccaag tgcgactttc ccaacaactt tcgataaggt gtaccgcagc
    19561 atcctgtatt caacccaccc ggataaccct gaagcaaccg taatgcctac cgtaccatag
    19621 agcttatcga tatgcaatag atatggagca tattgcgagg cacaataacc caatgtactt
    19681 gatagcgcaa cacgaataag tgcacatttt aatggtgttt ttgtatcatg catggcataa
    19741 aatgcagatg agaacaacct gctctgtgtt gttgcaaata aaccaacggt tgaacctgcc
    19801 aataccatcc atacaaaaaa ggtatcgcgt gttgtaaatt taccggtttg atatatagca
    19861 gctactatgc aatcacccag tataaaaaat ccagcagttg acggaactat ataaaatgca
    19921 atgcgtttga ttgagttgtt aattcgcttc tgtaatcctt ttgctacatc gcgggtattg
    19981 ccgcttacct ttgacatggc agggagctct gctgccgata ctgacatacc aaataaactg
    20041 attgggagca tatacagcgt ctgcgcatac gacaaccctg caagagcacc agttggcaaa
    20101 aggcttgcaa gcaatgcatc aatgtaagcg cttatctgaa caactcctct gcttataaat
    20161 accggtacaa aattttttaa tatagttttt acttctttag tcttaactgt aaacgatagt
    20221 tttatatgat ggagtatccc tatcacaacc ggcagttgca tcaacaactg cagtaaactg
    20281 ccaatgaccg agcctgcagc agcatatttt gcaagctcat cctgtgagga gctatcgcca
    20341 aacaaaatta aggtaatgat gataacaaca ttccataata ccggggcact gtacgataaa
    20401 aaaaattttc catgactatt taaaattccc agacaccacg ctgaaaaaac aagaaccccc
    20461 gctcccggaa aaagaatccg taccagagta atggtaagct cacgcttttg cccttcaaag
    20521 cctggtgcaa tgacggtgat aagcaaagga gtgataataa taccaaccag tacaagggtg
    20581 gaaattaata acgaaagaat gcctgcaatt gcatgtgcta cctttgctgc ttcgtcatgt
    20641 ttgttttgtg caatcaactt tgcatatact ggtataaacg aagcagaaag aacaccctca
    20701 ccaaaaagat tttgcaaaaa attaggaatc cggaaggctg ctctgaatgc atcagcagca
    20761 tccgagttac ctaaaaaatg tgcaaaaact ctatcacgaa taagtcctgc tatcctgcta
    20821 aggaaaatac cggttgccac cataagtgtg ttgaaaaagc ttttatcatg ttgtgttgat
    20881 gtagccatac cctgatatac taaatagtag tattaatatt aatttgaaaa cattgttacg
    20941 atggttgtat aacagtattg ttaggcacaa tttgtaaaga ataaaataga atatttatat
    21001 tctataccgg tgatttcaaa aatttcttga aattttcgga ataatattcc agcatagcta
    21061 taactcatac ggtatatagc atgtaaaaaa actaaaaata tacagcaagc ttgtagaaca
    21121 atgcaagcac tgttgtttta aaggtcaatg ctcataatgt tgcgctcccc ttctttttgc
    21181 taaagcgata gttgttgcag taactgcatt atgggctgat gatgatttta ttactttaat
    21241 ctctggagga aaatacgctc tatgaatcga attatcatct atacatgttt tatagtaact
    21301 atcgccacca tagcctttgc aggtgactgg ccaatatata aggggaatct tttttttact
    21361 ggaaacaatg atgagataat tgttcccggc aaccagatta aatggctttt tttagctaaa
    21421 gacaaggttt ttaatccggt ggtaagcgat ggcagaatat attttgtaga taaaaatgct
    21481 tttttatact gtatagatga agaagctggc accattctat ggttaaatga tgtgcatgcc
    21541 atatccgcac aatttaaagc atattctaaa tctgctggta aaattaaata cccgcttgtt
    21601 tacggcaata cggtacttgt gagtgaccca atagcaatct atgcttttga caaaacaaat
    21661 gggaaggtca tctgggcaag aacagggctc agggtggatg aaacaaaacc gcaaggatta
    21721 accggcatcg caactcaggt catggttgac ggaatttatg ctgacccaat cattgatgct
    21781 gatacaatct attatggaac acggaaagta tttgcatcca gagacattgc aaatggacac
    21841 atcgtatggg aaaataagga tattgctacc tatagtggtt ttccggtata ttatgaccaa
    21901 tatattatta ctcaatcaat gaattatgaa acaaaagctt ttactgtttt ttgtcttgag
    21961 caaagaacgg gaaaaccggt atggcagcgt acgttttctg tgccaattaa aattttacct
    22021 cctgttgtca ataaaaatgt ttatgttccc gtaaatgaca ccatttactg cctgaatatc
    22081 aaaaatggcg aaataatgtg gcaaaaaagt tataatagaa ctatcacttc accattaagt
    22141 tttactgaca ggaccatttt attttcattt gacaacaaca gtattaaagc tattaatcct
    22201 gaaaatggtg aagttgtttc aactattttt gttgggcaac aatcctctcc ttactttgtt
    22261 actatacgtg atgttattta tattgccttt aatgaagtaa ccgataaaaa tcctgttgca
    22321 aaagtaaagg caatgtatat cagcacatca cagctatggt gggaatttgt tgcacctttc
    22381 ccgggcggtg tatcccagcc ggttgctgcc gacggtatct tatttttacc agcaggaaat
    22441 tatctctatg caattggtgc aggatatatg ggaagcaaag gatttgatag tgcaaaaact
    22501 gcaactgata gtgataataa aaaaaatgaa caaaaatcta tgccagcacc tcgttattca
    22561 gaaaaagaac tatcacaaaa aactatcacc cctgaaacac aaccaaaaaa aacgtctgag
    22621 caaacacaaa agcaattgcc catgcgaacg ctgccagttg aaataacaaa tgaccagcta
    22681 agcccgctga aagctaaagc aacaattcac tataaagacg aagacggtga gtataaacaa
    22741 tcaactgctg ttaatgcaaa aggtgatatc actgtccccg ataaagacaa tgttgaagtt
    22801 atttttgaat cagatggcca tgtaccaaaa aaggtcattg ttaataaaag cgataagcag
    22861 ctaaacgtag tgctggatca gatacaacct ggtaaatcgg tggttattga taatatacat
    22921 tttgattttg ataaatggta catcaggaaa gattcagtaa ctatactgga caaactggtt
    22981 gagatgatga aaaaaaatcc cggcctaata gtagaggtac agggacatac tgattcgacc
    23041 ggcgacaaaa catataacca gaagctatca gaaaaacgag cagatgcagt tgcagaatat
    23101 attataaaac atggcataag tcctgagcga atcagctcca gagggtacgg agaaacaaaa
    23161 ccagtagcat ccaacgaaac aaaggaaggc aggcaaaaga atagaaggac cgaattttat
    23221 tttaaatctt aattggaaag atgcatatca ataattatca ataaagggga tatgagcccg
    23281 ttaaattcat tagtctttac cgtgtagatt atatccactg gtttcttttg tgctacaata
    23341 gcttcatatt tatctttgcc attccatgcc attgcctcaa gttttattgt tccatctaca
    23401 atgcatttta tatgtttagc attaataata taatagcttg caatagagca attgcgcgaa
    23461 ataaaagtaa aaggcttatg tcctgcacca tacggttcaa acagttttaa ttcatccaaa
    23521 aaatcaaatg atatatagtc aataggcaac tcataatcat agtgtatatt attgttacag
    23581 gtatcatact gttgtaaata atcatgcaat aacttttcaa gcacctctat atttttttta
    23641 tgtattgaaa atccaaatgc ctgctcatga ccgcctaatt tttcaaatat atgtttaaat
    23701 tgggaaacaa ttgaaaaaaa atcaaggtga ttacctaccc ttcccgaccc tttaacaaca
    23761 gtgctgtcag ttttattggc tatgactata acaggcaaca gggtttgatc agctattctg
    23821 tttgcaataa taccggtcaa tccttccgga atctcattag attgtatcat aatgattttt
    23881 ttattagcgt tagcactata gctttcgcta aatttgttaa ccagtgtttg tataatattt
    23941 ttccgttcag cattgagttg tattatttca tccaatatac cttttacttc ggaagattta
    24001 tccatcaaaa aaaattttgc agccaattct gtttttccaa atctaccagg tgtatttaaa
    24061 agcggtgata tatcccagcc aataacatct gcattaatgg gttttgcaat atgctcgcga
    24121 ataacgttta taccagtatg atacgtttta ttaaaaaatg aaataccagc ggttaccagt
    24181 gttctgtttt catccaccag cggcatcata tctgcgatag ttcccatggt taccagcggc
    24241 accacatctt ttataaattt catcatcttt tttgattgct ggaactgcat tttctgagcc
    24301 actgtaacag caaaatccaa tgcagaagga aaaacttttt ctggttcaat tttcaaaaga
    24361 ttttttttgt tcgatagttc tcctgaataa tcttgtaaat tgaataattt tgcatgctgt
    24421 aatgcattga ctgccgctac atcataatgt agtagtatcg tgttggtata tttatttata
    24481 atatgctgta tatcagatat atcatctact acaccacatt ctttagtgca gcctctgtgc
    24541 attactttat aatgatatgc attactgtca actgcacaaa taatgacata ctgttcataa
    24601 tatctggtat agcttaataa caaagcaata cataacttaa atgcaactcc cacaccggca
    24661 agttctttaa aaggataact gcaagtatgc aattttggat ttattattac agcatcagga
    24721 agttcatccg aaggttcatg atgatcgcat acaataacat ccataccatg agctttcgca
    24781 tatgcaattt cttcaacatc ccgtgttccg ctgtcaagcg ttatgatgca ttgtacatcg
    24841 cgctttctaa attcatcaat tatggggatg gttaatccat acagctcttc tccaactgga
    24901 tatcgataac aataatccgc acccagtaga tcaaaaaggt gtgatacaat agttaatgat
    24961 gtcaatccat caatatcaga atctgaaaaa atcccaaact ttattttttg ctgtatgcac
    25021 tttttaatta atacaatggc ttcattgatt ctattgaaaa gaaatggact ataacagtta
    25081 atgagttcag gattaaaaaa aatataagcc ttatagggag tgtcaatacc tcgtagtaat
    25141 aataggtctg ctgttacatc agtgatatta aaaagatgca ataatgtttt ttttgatagt
    25201 tcagctgttt tggtatacac attttccatc ttatatataa atactatata acatttatct
    25261 ttctattatt attttatatt tttttatctg cagccttctt tgccaaaaaa tctgctcttt
    25321 cattaaagtt gcgcataaca tgctcaatct taaagtttgg tatctgtttt tttaattcca
    25381 ttactttatc atataacttt tttatttgtg agttatttac ctgatattcc cccagaagct
    25441 gccgcaccac aagctcagaa tcactgcgta tttttaaagt tttagtttta aaataaattg
    25501 ctatcagcat tgcccgtatt aaagcaatgt attctgcctg attatttgtt tttttaccta
    25561 tatatgacga tactttccca acaaactggt catcattatt atatataact atgcctattc
    25621 cagccggtcc tggatttcct tttgatgcgc catcaacata cattgttaaa actgattcag
    25681 taggtatggt tgataacact tcaaatccat caaatagttc ttcatccgtt tctttcatct
    25741 ccattgggat ctgagtgtta atattatttt tcttttttat gataatcttg ctttttgatt
    25801 tttgcttatc aaattgatta acaatttttc gtgcttcttc aattatagct aatacatcgc
    25861 tactatcaac attagcaagc tctgcaatct tttctatact cttaccttca gctaataact
    25921 gcaatactct atcaatactc agtaacatta tttttccctt aaactatata gtgtaatttt
    25981 cattttccat ataaaccatg aagcttctct ttcagattac gtgtcatgct tattccgttt
    26041 tccaaaatga cttttattac ttttgccgtt cacaataaat gccagaagta ttatctcttt
    26101 tataataatt ctatatatgc tatgttatat aattattttt acattacgtc aactttaatt
    26161 acagcaataa aaaatagctt aaaaacaagt acaaaaaaga taccatttac aatattatag
    26221 ctaatttttt agatacaaaa acctcccaca attagtacag gttgccggtc ttgattttat
    26281 aacctcatcc tttacataca caggtacctg aaaatgacag cccatacaaa tgccatcttc
    26341 aagtggtaca attgcattcc cttttgtatc aataaccttt gcaaaacgca tacgtaaatc
    26401 cggtggaagc tctgatatcc ttttattaaa ctcctttgca ttatcttcag cttgtttttg
    26461 cattacttct aatttacctt gtaactgctg aacatcattt tcaatttgtt gcaaacgttt
    26521 cggcatctcc acatggaaaa tctctaattc accttctatc tgctctacat cttccatata
    26581 ctgtaaaagt tgttcttcaa tagtatcttt ttttttagcc agggtatcca gctcggtttg
    26641 cattgattca aactcgcgtt gcgattttaa agaatttttt ctgctatcca gcaattcaat
    26701 ccttgattct acatcatgca attccatttc cttttctttg attttcaatt taagctggtc
    26761 aactcttgaa cgcaaatcag acatcctggc tccaatgctc ttcatttcat cacgccagaa
    26821 atcaattgca tgacgtgaac gtgcaatctc agttttagaa tctaaaacct tattccatat
    26881 agcctgaagc tctaacatta catatatctc atttcccatt ttgtacacca caatgcatag
    26941 gattttgttc tttttctgaa attattaaac gaacatcgct acaatgcatg gcatttgtca
    27001 agtagttgta tagttttgaa tgtaaaaaat gaaggagggg tttttcggaa ctataatgtc
    27061 caatatcaat aaccgccaca tcatgattat tggcatagac tgcatcatga taaccaacat
    27121 caccggtaac aatgcaatca acgggatatg attgtatgat agcattgatc ttttttgaac
    27181 ctgacccatt aaaagcacct atcgttttaa tctttttatc cagattgcca taataaataa
    27241 ttgccatggt tttaagttca tgctgcaact gctcaataaa ttgcgataac aacatttcat
    27301 cctgcataca tccaatggca ccaaaaccaa ttccattaaa ccgcttatca ggaaataaca
    27361 ttttgatatc gttaagcctg acagtttttg ctaaaatatc ccagcatagt gcatctaaat
    27421 tggtatgcac tgcatataca ctgatagtat tttgaatcag ccgtattgtc atgaaaccat
    27481 gaacactgga tatggtaata ttttttaatg aacggaaaaa catggggtgg tgtgttataa
    27541 tacaattaca ttttttttca atggcttcat caatgatctg tggggatata tccatagcta
    27601 taagaattcc ggtaacatta ctttcatcaa aagctatctg tcgcccggaa ttatcatatt
    27661 cctcctgata cattagagga aactcattat ccagaaaatc aaatagctgt ttatgaggta
    27721 tattcatgca atctcctttt tatgctaacc actatctgtg ctacatcaag agggttttgt
    27781 ccagcaatat ataacaacgg atcatcatca acaacaatca taatctgcga tttatcaaaa
    27841 cccgtgttca taagttcatc aggagcagtc acctgtataa ccggcacatc catagcatga
    27901 gctatcgccc tgatggcatt actatttttt atacttatag ctgtgcgata gttaccttta
    27961 attttctgca tcatatcagc aagcggatgt tcaacattac atgctgcgtc ttttatcttg
    28021 aagactgaat cctgatgggt tatgatacct cccggaaaga gagcaaaatc agtttgattt
    28081 ataggatacg gaagggcata cccaatattg gatgaaccgc tcacaaggat atcgccgcac
    28141 tgtgcagcct gaataacaga aaatgcttgt tctacctcat gtataacttc ttctttatag
    28201 ttattattaa caatatcaac taaatgattg gtgggaccat gccctttacc caatggcaat
    28261 gaaaattgta ttgcttttgt aataaatgct tttgcctttg ctatggcttc tatggcgttg
    28321 tatccattag ctaaatatga tgcaatcgcg gcagaaaagg tacatcctgt accatgtgtg
    28381 ttttgtgtat ttatttttgt gccctgcaaa atggtatagt cgttcccatc ataaataaca
    28441 tcatcggcac tatcattcag atggccgcct tttataacga catatcgcgc accagatgaa
    28501 tgaatttgct ttgcaacctt tttcatatca tctattgttt gtatggttaa accagttaaa
    28561 acctcagcct catggagatt tggagtaaca acatgcgcca ccggtaaaag aagattaatc
    28621 acagcactga tggcatcgtg agaaagcagt gttgatccgc cctttgcaac catgacaggg
    28681 tcaacaacag tatacggtat ggaatatttc tttatattcg atgctaccaa tgcaacaatc
    28741 tcagagctgg caagcatacc agttttagcc gcatgtatgg ggatatcaga tgctactgca
    28801 tcaaactgtt tttgaatcat atcaagaggt accggcatat aatcacttac acccaatgta
    28861 ttctgtgcag tgattgctgt gataatcgtc ataccataat tacccagagc ggttattgtt
    28921 tttacatccg cctgtatacc ggcgccaccg ccagagtcag aaccagctat ggtaagcaca
    28981 ttattaatat atgatgcagt atccatattc catgtcatgg tatatcaaca gttatactac
    29041 aataaatgag ttctatatat agtataggtg aaatcacagt ttataggtaa taggtacacc
    29101 ataattttca agaaaaattt gtaaaaccta ttacattatt ttgcagttct tgttgttact
    29161 attatctata caactatagt cttaccctgc ttttctgtca tggtgatggt gcatggtgtt
    29221 tgcccattga caatcctttt agcttgactg atttttgatt atgtgttctg ctttcttaac
    29281 tttttatgat tacttctttt agtttgttgt ttattcatat tgtaaattgt aatcgtttgt
    29341 gacagctatc tattgcaaca aacattttta tatgctctga ggagtgtact ccatgaacta
    29401 caatgaaatt tttaaactta ctgctgatac acgctggaat gtagttgctc aggatgaaaa
    29461 tctatggcgc gttggtatct accatccgga atacaacagt gcccatgaaa ttgacaggtt
    29521 agaaaagcac agttgccctg aactttttat ttgtgtcaat ggacagtgtg gcttgctatt
    29581 gcgtgaagga aactgggaac atcagctcat catgaacccg cttgaagcta ttatggtaac
    29641 cagttatcac aatggattta aggttgacaa agatggcttt tttatagtag ctgaacgtac
    29701 tgattttaca accaacttta ttgaacgaga taaaccgtga aaccagatgc aaatacaacg
    29761 gtatactgta tcataggcaa tccagtaagg cattcattgt cacctgtcat gcacaatgtt
    29821 gcctttgaac atcttcatat aaatgcaatc tacgttgcat tccaaccaca gtctgcacaa
    29881 aaagcaattg aagcaatgaa aacattgggg atacagggag caagtgtaac gataccgttt
    29941 aaggttgaac tggcatctct atgcgatgag gttgacgatc tggcactgga aattggttcg
    30001 gttaatactc tcatcaatgt caatggcaac ataaaagggt acaataccga tggcaatggt
    30061 ctcatcaaag cgttagagca tcatgctgtt gattatgtac ataaaaatgt tttaatcatt
    30121 ggcaacggcg gttccgcaag agctatcgcc tacacattag cacacaacaa tgcacatatt
    30181 atcatctgtg gaaggaatga aaataatgtg atgcatctgg tacatgatct acagcatcat
    30241 ggtatatctg tcaatagctg tttacttcat gatttgcacc ctgatacaac attgcctttt
    30301 gacatcatca tcaacactac tcccgttggc atgtcaccgc atgcggatac cagccccatt
    30361 aatgaacatt gtatacatcc ccatcacata atcgttgata tcatttattc cccgcgtgaa
    30421 accatgttga taaaaaaagc acgtaataaa ggttgtaaaa ccataaccgg tgaatatatg
    30481 ctgctttatc aggcatgtat gcagtttgag ctatggacgg gaaaaccagc cccatttgaa
    30541 attatgtata acgcactaac tcaggcattg cagtaaccat gctgccgcct tttttacaat
    30601 cgcttgtgaa taatgaatcg gttcattcag cgcgctatgt acactattca cccgatgcta
    30661 ttgttacatt gctgagcctg cttcataatc cgcatacagc cattaaaaca atacatattg
    30721 ccggaaccaa tggtaaaggt tccacggcat atatgattgc tgccatgttg caggcagcag
    30781 gttaccgtgt tggactttat acctcgccgc atcttctgcg gtataatgaa cgtatcactg
    30841 tcaatggtaa tgaaatagct gatgaaacaa tagcacattt cagcactatc attgaacaac
    30901 tatgtacaaa gcataatctg cacattacat tctttgatgc atttaccgcc atggcatttt
    30961 tgtactttca aaactcagtt gatattgcag ttattgaaac aggacttggc ggaaggcttg
    31021 actcaaccaa tgttgtaact cctcttgtga gtgttataac acccattgcg tatgatcaca
    31081 ctgcaatact gggaaatact ctgcaacaaa tagcatacca gaaagcgggt atcataaaac
    31141 aaaacacacc tgttgttatt gcgcaccagc atccagaagc gcttgcaaca ctgcggcatg
    31201 aagcggcatt aaacaatgca cccattattt tgtttaatac caatattcac tgttcaataa
    31261 catcaaatca tcccgtgtta acatgtgatg ttgtgtgcaa tcatccgctg tttggcggtt
    31321 tgcaaaaatg tagcaatgtc accatcccgt tagctgggag ctttcaagca tacaatgctg
    31381 cggttgccat agctgcagtt ttacttttaa aaaattatta ttgggcgata gtcactgatg
    31441 atataatcaa agctctatca accataacca tcccgggaag gagccagttg tttggtgaag
    31501 atccattgat actgtttgac tgtgcacaca atccggaagc gatgcaaacc acagtagaag
    31561 ccctgataaa cacctattcc gggtatgatt gtatattttg tgtaagcttt atgaacgaca
    31621 aggatatcca ctctttgcta actatcatac agcgctatac acagcatcca gtatattatc
    31681 tccccctcaa tgacagcaga agctattacc ctgatgatga agttatcaaa aactatcatc
    31741 tcatcattac tgatgatata catttaccag taactatcgc caaaaaaagc cctggaaaaa
    31801 ctatggtgct atttaccggt acattccgat tatatccatt ggttcaaact attcacaatg
    31861 cttgatgtgc attaacacag caatgcatcg atattgataa taaaaattca tcaattgtca
    31921 ttactgccaa atgtacgcac ctgagcactg ttcatagata ataacaatta taaaaaatgg
    31981 atgtgttcaa aaaatatttt ataataatgt attgatttta gtaatattat ttttttatga
    32041 tacaattata ttatggaatt cccaataaaa aaaggatatg ccatataaaa aaattaattt
    32101 ataaaggaga ttatttaaaa caatggatct atcagaacta ttacccaaag aacatctaat
    32161 ttccattaag agtacagaaa agcaactggt aataaaagag ttaattcaca agttgcagga
    32221 attagggaaa ctggataatg ccgacaggta ttatacacag gtcatacatc gtgaaacact
    32281 ggaaaatacc ggtgttggta atggttttgc aatcccgcat gttcgcaccg attcagtcaa
    32341 aaaattgctt actgtatttg gaatatgtaa tgaaccaatt gaatacgaat cgtttgataa
    32401 acagccggtt aaatatgtca tgctcagcat atttcccaca tcactcagca ccaagtatct
    32461 ttacctggtt ggtatgatgg cgcgaatatt ttcaagctca gagaaacgaa gtaaaatcga
    32521 tgaagcacga acaccgtcaa aaatatatcc aatactaaca aaagaagcaa aactttattt
    32581 tgaatcgcta accgaaatca ataaggaaga tgaacatatt gagaatcttt ctggcattcc
    32641 ttccactgac cttgacctgc tcatacggct tgaccagctt taccgcctgt atgataccgg
    32701 cgatacatcc gaagcattaa caagaaagat taatgaatta cggaaattta ttgataaccg
    32761 ctcactttca tattatgaac gtatgagaaa aaaatgtcaa aatccttttg ccattctgga
    32821 aaaaaatgcc tgcggtggat gccacatggt tataccgcca gttgaaatgg caaagattaa
    32881 atctaaaaag caccttgcag tgtgcaccta ttgtggaagg tttttaatca cagtataaaa
    32941 tagtataaaa atgtttaatc attatattat aacaggtatc tgaaacggta acaaaaccat
    33001 tcttttataa gttgtgtaga cgatcttcgc tggtattgct caaatactat ctctttactt
    33061 tgtttaatat cttttaaaaa ctgtttcttc atcttattcc caaaattctg atcaagaaca
    33121 atagcattaa cctcaagatt agcattaaaa ctgcgtctat ctaaatttgt tgaacctact
    33181 gttgaccata tatcatctat aacagcggtt tttgcatgta ataccgttgg gatatattca
    33241 taaatacgga tatgattttt aagaaaacgt ttataaaggt atctgcttgc atattgaaca
    33301 aatgggatat cactaaccga gggtaatagt atcctgacat ctacacctct ctttgctgca
    33361 tggatcaatg ccctgctaat tttactatca gggacaaaat acgcatttgc tatatagata
    33421 tatttttgtg cattggcaat tgctgctaaa tatgtctggt aaatcggctt tacattttta
    33481 cgagaataac tgcttaaaac caatacaata gtatcacctt tatgattaat aacaggaaaa
    33541 tacctggcta tatcaacaag agcgccgccc tgtctatgcc agttttccag aaaaaaatac
    33601 tctaaatcac gtactgctgg cccttcaatt ttcaggtgag tatcccgcca attttcacct
    33661 ccatattttt ctccggcata ttcattccct atattcatcc cacctacaaa tgctgtgctg
    33721 ccatctacaa ccatgagttt acggtgatct cgtagatata aattgaaata tttacgccag
    33781 ggtataatgg gatggaactc aattaattca actttgtttt ttctcaaata ttcaaaaagc
    33841 tcgccagaag tattaatgca ccccacagca tcatagatga tattaacatc tacacctttc
    33901 tttgcctttg ctgctaactt acgtgcaact ttccagccaa tatcatcact attaaaaata
    33961 tatgtttcaa gattaatact taatgttgca ctatcaatag cagcaaataa ttcatcaaaa
    34021 aataaatcgc cattgttgat aagggtaacc ttattgccgc ctgaaaatcc ggatatacca
    34081 tactttttta ttgactttaa aaatttaggt aaaaaaaatc tatttttgat tttatgtata
    34141 acatcactat aactgattct atgtcctgaa cctttgaatc tatggtttaa acgtccatgg
    34201 gttttctttt gtgatttttt catgaaatga gaacatattt ttatttataa tgaacaactt
    34261 caggagtatt tatatctcca actgttccat ttttctcagg tttaaaatag attgtttggt
    34321 tcttgatgcc agcatacacc ttatggcatc ctttaaattt cccatttaat atctcaaccg
    34381 ccaatctgtc ttcaatctcg ttttgcacag ctcttctaag atatcgcgca ccatatttgt
    34441 cattaaaacc tttactgaca agatagttct ttactttatg cgatactaac agttcaatgc
    34501 cctgctctgt taaatgttca ttgacctcct gtaataataa atcaagaatc tgccgtatat
    34561 ggtgctcatt caacttatgg aaatggacaa ctgcatcaat tctatttaaa aattccggac
    34621 tgaataatcg tttaacttct tcgtcaacat tctggaattg aatttgattg gcattgattt
    34681 cattaaatcc catcttacct atcttttgga attcacgatt accagcatta gaggtcataa
    34741 taataacagt atccacaaag cttgttacag aaccaaaatt atcggtaagt tcgccttctt
    34801 caagtacctg taataatata ttgaatacat caggatgggc tttttcaatt tcatcaaata
    34861 gtatcacgga atatggcctg cgttttacct tttcagttaa ttgtcctcct tcttcgtacc
    34921 caacatatcc gggaggagcg ccaattaaac gcgatacaga atgtttttcc ataaattcgg
    34981 acatatcaaa cctgataagg gcatcgtcat catcaaataa aaactcggct aatgctttag
    35041 ccaattcagt tttcccaaca ccagtagggc caagaaaaat aaatgaacca ataggccttt
    35101 gagcacttcg cagccccgtg cgagagcgtc gtattgccct gctgatgaca ttgattgcat
    35161 tatcctgtcc aataattcgc ttatggagct cctgctccat ctgtaataat ttctgggatt
    35221 cggactcctc aagcttttct acaggtattt ttgtccattg tgcaacaacg gtagctatct
    35281 gtttttcatc aactaccact gcatagtcat tgatgcgctg ctgccaatcg cttatcttct
    35341 gtgaaagaag agcttttctt tcaatgattg tgtcacggat tgatgctgcc tgttcatatt
    35401 cctgagcctt aacaagatta tttttacgat cattaagctc ttcaatctcc tgttctaata
    35461 attcaatatc aacaggcctt tcacagtttt ccaatcttgc ctttgaacct gcttcatcaa
    35521 taacatctat tgccttatcc ggtaaatatc tatcatgtat atatctgttt gcatacagaa
    35581 cagctttcct gatagcttca tcggtatagc gcaccttatg gtgaatttca tagcgctctt
    35641 ttaatccaaa taaaatttca acagcttcat cagccgttgg ttcatctacc ataactgttt
    35701 gaaaacgccg ttcaagtgca gcatcttttt caatgtatat acgatattca ttgagtgttg
    35761 ttgctccaat acactggagc tcaccacgtg ccagcgctgg ttttaaaata ttagcagcat
    35821 cgatagctcc ttcagcagct cccgcaccta taatggtatg aagctcgtca ataaaaagga
    35881 taacattgtc cgagcctttt atctctttca tgatgcgttt taaacgttct tcaaattcgc
    35941 cacgatattt agtaccagct acaacagaag ctaaatccaa tgataatact ctcttgttaa
    36001 aaagaggttc aggcacatca tgatgaataa tacgctgtga taatccttcc acaattgcag
    36061 ttttacctac accagcttca ccaatcagta caggattatt ttttgtttta cgggataaga
    36121 tgcggattac tcgttctatt tcagtatcac gccctattac agggtccaat ttatcttcag
    36181 cagccaatcg tgttaattca atggcaaact catccagggt aggtgttttt gttttatcat
    36241 tacctttttg ctgtgtgctc ccatcaggag taacacccaa tatcttctgt acttcacttt
    36301 tcaaaacatt ataatctata cctgcacgga taagcgtatc aatgccagag catgttccat
    36361 ccctgaaaat agaaagcaaa agatgttctg taccaacata gttgtttttc aatttacgag
    36421 cttcatcttt tgctatttca ataatccggg taaaacgagc actcagcgga agattgccca
    36481 gtattattgt agaacctgac ctccgaattg actgctctat atttctgcgt agtatttcaa
    36541 aatttactcc catatttttc aatatgcgtg cagcgacgga gtcatcatcc tttaacaggg
    36601 caagcataat atgttccggc cctaaagcat cagaatttaa ccgccgccct tctgcctgtg
    36661 ccagaacttc taatactttt cgtgacctct tggtatagtc aaacatcttt ttttactcct
    36721 tatctccgaa tctctttcga agatatgctg ctctgaagga atcaccttct tcagatgtgg
    36781 caaaaatttt tcctgctatt ttctgaagat gtgatacctg tatatgtatc attaaatcat
    36841 taattgcaga aagttcaata ttttttaata cggataatac aacacccagc cgaataagcg
    36901 ataaatgctc aatagcttcc gcataattca ttgtccttga aaatgcaaca attgcatatg
    36961 atctccagat catatcttcc agcatatctt tatgatgctg ataatattct tcacgggcat
    37021 tattttcaag atcaataata atactgataa ccttatcaac ttcttctata atatctatct
    37081 cagattttcc taatgttgct ttattgctga cgatatatat gctacctact gtttttgttg
    37141 catcatggac aattcctttt atctgagcct gatattcacg tacaatctta ataacctcac
    37201 ccatattttt tgttgctgtc aacatgggca aatgcaacat tgctgaaaca cgcaatcctg
    37261 tccccacatt tgaaggacag gtggttaaaa atccaatatc atcattgaaa gcatatgaaa
    37321 tgtacttgtt taattcatca tcaatttcat cggctaattt gaaagcttcc agcatctgta
    37381 atccaggttt tataacctgt atacgtatgt ggtcctcttc atttataaga atggaaaaat
    37441 tttgctgatg atcaaaaaca acagagctgt aatcacttaa ttccatctca tgagtgatga
    37501 tatcacgttc acataaaaaa cgtcggtcat cagaactgca atcacgcaga ttaacaaatt
    37561 gagtattttg aaataacgtt gaagcaataa cagcctgtcg cactatggtt tccagtatgg
    37621 ttatatcaga ttcccgcatt ttagtggcaa aaggtaacga tggcaaattt cttgcaaacc
    37681 ttacacgggt tgtcatagca acatctgcca gaggacctat tcttgcccag aagggtgatt
    37741 gttccaacaa ctgttcaaac attatgcccc ctgttgattt ttcagtttta ttatctcatc
    37801 tcgtaatttt gctgcttctt cataattttc attttctact gcaacttcaa gcatattttc
    37861 aaggtcatga agcgataacg ttgcaacatt tttcaaacgc attgaatcaa gcattacctt
    37921 tgattttcta tgctctatat atttcagtgg cattttccca atatgaaatg ttgaaccatg
    37981 atatcctgat aagattggtg ttaaagcttc cttaaatgag ctatagcaat ctgcacaacc
    38041 cagcttccct gttcgtttat aatctatgag tgtccatcca caggtggcac aggtgttact
    38101 atcaaccata tcattcatat cattcatatc ctttacatcc agaaaggaca tcatatcaga
    38161 aaccgtaaga gaaaaaccac cgccgtgtga gcttagaccc atatcgcggg cacagttatc
    38221 acacaaatga acttcagttt tttcattcct gataatctca gtcaaatgga ctgttgcttc
    38281 tcttagttta caccgttcac atatcatagt tcatcttcca tagtaactat caaacactta
    38341 cacatccttt catcatatag tatactataa aataagttta tatgcaagcc catttttaaa
    38401 tcattttaat aaaattttat ctttttacta aaaattacca aaatatattg acattataga
    38461 atgatagtat attgagtgag caatcgggtc aaaccgattt attttttttt atgggatata
    38521 ctttatggca gtatcaaaaa aattacaaac attgatagaa caggataatc aatatctttt
    38581 tcaaaattat ggcacccggt taccggcttg ttttataaaa gggaaaggga gcgaactctt
    38641 tgaccaggat ggcaaacggt atattgactt ttttgccggg attgcagtca attcgttggg
    38701 atataatcac ccggcattga atagcgctct acataaacag attgatgcta ttatgcattc
    38761 atcaaattgg ttcttcaaca aacagcagat agaagccgct caattgcttt cggaaacatc
    38821 attcccgggc aaaacccttt ttacaaacag cggcaccgaa gctaatgaag ctgcaataaa
    38881 attagcacga aaatatggaa tatcccaatc caatgataag tatcatatca tatcgttcac
    38941 aaactcattc cacggcagaa cgtttggtgc catgtcggca actgctcagg aaaagattcg
    39001 ttcagggttt ggcccgatag ttcctggttt tatctatgtg ccgtttaatg atgtcacaca
    39061 atttaaaaag acactggaag ccaataaagg aaaaatctgc gcagtgataa ccgagcttgt
    39121 tcagggcgaa ggtggcataa cagttgccga tagttacttt ataaaagaaa tcagctcatt
    39181 atgtaaagag aacaatattt tattaataat tgatgaggta cagacgggta ttgcccgtac
    39241 tggcaagatg tttgcctatc agcattatga catacagcct gatatcatat cgcttgctaa
    39301 aggtcttgga ggtggtgtcc ctatcggtgc cattcacgct caaaatattt tggtggaatt
    39361 ttttccaaag ggaacacacg ggacaacctt tggcggcaat cacctggctg ctgcagcagc
    39421 aaaaactgtt ctggaacatt gcaaaaaaga atccttgctc aacaatgtca ataccatggg
    39481 caaacttatt gtagagaaac tatcgcttat gaaaaaaaaa tatcctaccc ttattattga
    39541 tgtgcggggt attggtttgc atattggaat tgagctttca atggcagggc aacccattgt
    39601 tgttgaagca ttaaaaaaag gtttaattat caactgtact gccgaaaagg taattagaat
    39661 aatgccgcct ttgaccatta acaaaaaact tgttactgag gggttgtcta tccttgatag
    39721 tatttttgca ggagtaacag catgattggt aaaattccta aggtaaaaac aaaagatttg
    39781 ctcagtatta cagacttttc taaagaagaa attattgcta tctttgaata tacacaaaag
    39841 atgaaagacg aacttaacca tggcaaaaca cacaattttc ttgagggtaa aacactgggg
    39901 atgatttttg aaaagacatc aacccgtaca cgagtttcgt ttgaggttgc aatgtttcag
    39961 ctgggcggtt atgcattgtt tttaagcaga gacgatatac agttaggacg tggagaaaca
    40021 gttggtgaca cagctagagt tttatcacgc tatttagatg gaatcatgat acgatgttat
    40081 gctcatgcaa cagcgctgga attagctgaa aatgcaacca taccggtgat aaacgggcta
    40141 accgatatgt ttcatccttg ccaggcatta gctgattttt atacaattta cgagcacaat
    40201 aaaaatttta aaaatgagaa attggcattt gttggtgatg gtaataatgt tgctcaatcg
    40261 ctcatgctta ccgcagctat gctgggtgta aactttgcgc tggcatgccc taaaggatat
    40321 tatcctgcaa aaaatgtagt tgagatggct ttttcaatag cttcaaaatc aggcagtgtt
    40381 attactctga caagtgatat tgcactggcg gttgaacagg ctacttatct ttatactgat
    40441 gtgtgggtta gcatggggca ggaaaaggaa gcgtctcgac ggagaaaaaa ttttgcaaaa
    40501 tacaaaataa caatgaagat tctggataaa tgtgctccca attgcaaagt aatgcactgc
    40561 ttacctgccc accgcggtga agaaattacc gatgaggtca tagattcacc ccaatccata
    40621 gtattggatc aggccgagaa caggctccat gtacaaaagt ctattttatg tgctttaatg
    40681 gcaaaatagt actgcactta attgtatcca ttttttctat aaacagcaac acggtttctt
    40741 ccgctttgtt tagcctgata taatgcctca tcggcatgtt ttatgatcat ttccagctct
    40801 tcgtctatat ccgccaatga ggcaatccct gagcttatgg ttactgttat taaatggtca
    40861 ttgtcgttaa atgtacattc ctccactata ttcttgatcc tttcagctac tatcacagca
    40921 tcatctggca ttgtctcagg caaacagatg ataaactctt cgccaccata ccgtgcatag
    40981 atatccaggg tacgtagctg tgatacaacc ttttgtgcta ttttttttta aaatatcatc
    41041 acctttaaga tgtccatagg tatcattgat atttttaaaa tgatcgagat caaatataat
    41101 aatggaaaaa ggacgattaa ctctcctggc acgtgctaat tcacgcgaag ctgtttcaaa
    41161 aaaatatcgc ctgtttgcaa tgcttgttaa ttcacatgaa cttgtgcagt ttttactata
    41221 accatcaata tttagcttga ctataatgcc ctatacttca acgtaccaat atactattaa
    41281 gaattatgta aattactatc gtttgctatt gaggtacagt atgagtatta aaaaagttgt
    41341 gctggcatac tccggtggac ttgatacttc ggttattgtt aagtggctgc tggaaacata
    41401 taaatgcgag gtcatctgtt ttgctgcaga cgttggacag gaagaggaac tcaatggatt
    41461 ggaacaaaaa gcaatcaata ccggtgcatc aaaatgttat atcgaagatt taaaagaaga
    41521 gtttgttcgc gattttattt tcccctgtat taaagcaaat gcaatctatg aaaatagata
    41581 tttgcttggc acatccatag cgcgccctgt tattgcaaaa aaacaggtac agatagcgtt
    41641 acaggaaggc gctgatgcag tatgtcatgg agcaacggga aaaggcaatg atcaggtacg
    41701 ctttgagtta accttcaaag cactggcacc acagctaaaa atcatagccc catggcgtga
    41761 gtggaattta acttcacgtt catcgctttt tgattatgca gcaaaacaca atatccccgt
    41821 tccggtaaca aaagataagc cttacagtat ggacagaaat ttgcttcata tttcctatga
    41881 aggtggcata ctggaagatc cgtggcgcga acctgacgag agcatgtttc tattaactca
    41941 atcaccccaa aaagcaccgg ataaaccaac ttatatagaa atagattttg aacacggcac
    42001 acctgctggc attgatggta aaaggttgga ccccgttacc cttatgaaaa ctttaaataa
    42061 aattgcaggg gaaaacggtg tgggcaggat agatattgtt gaaaatcgtc ttgttggcat
    42121 aaaatcacgt ggcgtgtacg aaacccctgg gggaaccatt ttgcatgtag cccatcgcga
    42181 tcttgaagct ataacactgg acagagatac tcagcattta aaagacaggt tggcaataga
    42241 atatgctgag cttgtttata acggattgtg gtttacaaaa cgtcgggagg cactggatgc
    42301 atttatcaat gaatctcaga agaatgtgac gggcactgtt aaggtaaaac tttttaaagg
    42361 aagctgcatg gctgtaggac gcaaatcacc cgtgacttta tacgagccag acatagctac
    42421 atttgacgat agtgctctct atgaccacag agacgcaaca ggctttataa acctttttgg
    42481 tttacaaaca aaaattgaag cgttattaaa cctaaaaaga aaataatatg ggcgatagtt
    42541 ccttaaatat ggtagagtct acttatgttg accgagtgcg caatcattct ttatttaaag
    42601 aatcatggat agcatttgca agcgattatc ccccatcaca ctgtttatta gacttcaacc
    42661 aactgccatc aactgatgta gcattgcacg gtggcgattt aggatttgct ttattatgga
    42721 ttgttcaatc agtattaatg agatgcgttt actttgatat aaaccagtta caaccatggg
    42781 aaataccagc attaacaccg gttacaatag gtgccatggc tcacaccgaa cctgatactg
    42841 caagaatgac agcacggtat atcgataaca atacgctttc actatccgga cataaaaaat
    42901 atattactgg cggcaaagat gcagatttac tttttgtcac tgcaagaagg actggtgaag
    42961 ataaatttac aaaaattgta tatatacaaa cataccagct cccggaaaat gcattgcaac
    43021 agcttgacat gtcaatttta ccaacaatta accatgctac ccttactctg gacgattttc
    43081 cctgcaacgc tttacaatgc agtccggtga aggataagga gttacgtaaa attttaaaaa
    43141 aatggggcat ggtagagcgt gcattgatag gtgaagcata cataggctat ttatgttacc
    43201 tggcaaaaaa tataaccatg cctgatcaga atgaactcat agacgaagtt gaaaatctgt
    43261 tggcattaca aaaaacattt acccgtcaac aattagaaaa tgcattttct aataattata
    43321 tagatacatt gggtgacgta gcacgagtat tgtctgttac tcaaaaaatt tccaattact
    43381 gtatggaaaa tccacaaacc actggtgatt ccattacttt acgtctccat gatttaaatc
    43441 tatttaacaa gattagatta taatactttt catgatgaat acacgctata aatactgttc
    43501 agtaaaatta tttttatata aagttcttga taattatgct atatttgtag caatggtatc
    43561 aacttataaa acaaaggctc tattatgttt acctctgttg agaatacaat ccatgagttg
    43621 gagaaacaaa aatatatttg ttccaaagac atagcaacta ctgtatacct tgcattaaaa
    43681 atggaaaaac ctgttttagt agaaggacct gcaggagttg gaaaaactga tttaggcaaa
    43741 tgtattgcaa agtctttagg atttgaatta atacgccttc agtgctatga aggtctggat
    43801 gaagcaaaag cattgtatga gtgggaatat gccaagcagc ttttgtatac tcagatattg
    43861 aaagataaat tatcaagaat tcttgaggat caggctacat taaaagatgc agtaaataaa
    43921 ttgaccagcg aagaagatgt ttttttctca gaaaaatttt tagtagcacg ccctatacta
    43981 aaagctatta caaatgatat aacctcatta ttgctcattg atgaaattga taaatccgac
    44041 tctgaatttg aagctttttt actggaggtt ctttctgatt atcaggttac tatccccgaa
    44101 ataggtacta ttgttgcaaa gaacaaaccc tttgtattac taacctctaa taacgcccgt
    44161 gaaatgtcag atgctttaaa acgtagatgc ttacatcttt atattgatta tccaacaaaa
    44221 gaattagaag aacggataat acaactaaaa gttcctgaat gtggcaatta tctcattaaa
    44281 gaaattatta atgcaattca tgaaataaga aaatttcctt taaaaaaaga cccaagcatc
    44341 agcgaaacac tggattgggc aaaagcacta accttgatgg gctgtgaagt cctgaacagg
    44401 gatatagcaa ttgatacatt aaactttatt ttaaagtatg aaaaagatat tgagcttgtg
    44461 aaaaagaatc tgcattctat tattgttcac taataatgta tgttccacaa taataatatt
    44521 ataaaaaaca ttataattga agaagacaag gccaaaggag ttttttttga atttgcagaa
    44581 gttttaaaaa aagcaggagt tgatctatcc attgttgaaa taatggatat gatgaatgca
    44641 atttcacata tatcaattct ggaaaaagaa gtttttaaac aaacattagc taccactctt
    44701 attaaagatt atactgatat tcctgttttt aatcaatgtt ttgaacaatt ttttgagcga
    44761 aaaaagggaa atgattcttt attggatatc tacactctgc atgcgcattc tgaagatttt
    44821 atttttacac cggaagaaac atcgttgctc aattcattaa ttgacaattt tttagaatca
    44881 catcctcaat ctttttttaa aaaatcaaac gagtatctta caaaaatttt cattgatgaa
    44941 gaactatcat ctgaatctgg cggtgctgtg ggattgtcgt tgtttaacaa gcgctcacaa
    45001 tttgcatctg ctggtgccaa tgattctgat taccaggaac tatcgcccca actatttgaa
    45061 gtcattatgg gtatgattag aaaacgcatg tttgagcgaa ccatgggcag tactataaaa
    45121 cagcgtgagg attatctttt aaataaatac atatatcagc taaaacctga tgaaataaaa
    45181 gagatgcgag aattaataaa gcgatttggc cagaagatga aaaacaggat atcattacgg
    45241 aaaaaaaggg taaagcgagg aaactttgat ataaaaaaaa ccctgcgcag gaatcttcaa
    45301 tacggtggcg ttccatttaa aatttttcac agggatagga aaattgacag gcctcaactg
    45361 gtggttttgt gcgatatttc aagttcggtt aaccaatatt caagatttat gttgctgttg
    45421 acgtatacgt tgcagtcgct ttttgcaaag gtacgttctt ttgcatttat cagtaacctt
    45481 gttgaaatta ccgacttgtt ccgggaaatg gatccggaac gagcattaaa ctcaatattc
    45541 agcgatacca attttaccta tggttggggc agtaattacg gtaacagctt taatcagttt
    45601 attcaaaatt acagcgatgc acttaccagc aaaacaactg ttttagtttt aggtgatgcg
    45661 cgtaacaata atcaggaccc gggattagat tcatttatta aaatgaaaga gcgatcccgt
    45721 aatatatact ggcttaatcc tgataagcaa catttgtgga attggagtga cagtatagct
    45781 aacgtttata ttccctattg ttccgagatg cgtgaggtac aaaacttttt agacctgtca
    45841 cattttattg ataccctttt tatacaaaaa agataagtat catggaatac tgctggtaga
    45901 ttactttatc aatctatttg cttgacataa acacaaacat atcaataaat agaccaaaca
    45961 tttcatataa ttataacagt gagcattatt taattatgga aaacaaggta cacttattaa
    46021 ttattgatga cgaagatatt atcttaaaaa gttgtcaaag aattttacgc aaacgagaag
    46081 atttaaccat tgatactgca accaatggac gtgagggact tgaaaaggct aaggcaaaaa
    46141 aatacgatat tgttattacc gatctcaaaa tgcccgttat ggatggcatg gaggtattaa
    46201 agacattgcg caaagaacaa ccagatgtca cggttattat atttaccggt tttgcaactg
    46261 ttgatacaac ccgtgaagcc ctgaaattag gtgcatttga ctatatccca aaaccattta
    46321 cacctgatga attgagtaat gttgttgata atgccatcaa agcgcgtcaa aataaagcag
    46381 agtctgccat gcttgatctt ctggcgatag tttctcatga attaaaaagt cccgtttctg
    46441 tagttcacac aacggctgaa acactgtata aaggatactt tgggaattta tctcccgacc
    46501 agcaaaaaat cattgaaaca atacttcgca actgtcagta tttagaagat attatccgta
    46561 attacctgga tttatcaaag atggaattgg attcccttga atcttttagt cagcagatag
    46621 acttatatga agaagttatc aagcctatag ttgaaatgcc ggaaaacact aataatataa
    46681 aaaacatgaa attagagctt aacatacagg taaaaccaaa aataatgggg gatgcaaatc
    46741 tactaaagat tgtagttacc aatttaatta ataatgcaat aaaatatggt caggctaata
    46801 ctactattaa catagagtta acacaacaaa ataatgaata tgtcttctca gtatataacg
    46861 aaggtgtggg aatacctgaa gaagatatgg aaaaattatt taagaagttt tcacggttaa
    46921 agcaaaaagg cactgaaggt attaaaggtt caggcttggg attgtatata tgcaaatcaa
    46981 ttattgagaa acataagggt aaaatctggg ttgaatcaga atatggtaag tgggttaaat
    47041 tttcatttag tttgccatta tcatgaaaat tatgcttgaa attaatatta acctttttta
    47101 tattattcaa gcaaacgtaa aacttaaaac cattttggga gcttttaatg gagatagaat
    47161 taaaggatat tggtgaacat aaagttatat ctgttagcgg ggaggtggat ctttataatg
    47221 tcagcgaatt aaaaaaggca ttattcagcg taaccgatgg tacttattcc agcgttgtcg
    47281 tagatttaaa agatgtaaat tacatggact catccggtat aggcgcactg gttgcaggtc
    47341 aaaagaaaat gcgtgcccat aatggaaaat ttgcattaat caacatacat gacgatgtat
    47401 taaatatatt gaagctggct acgcttgata ggttttttag catctatgaa gatgaaaatg
    47461 atctaattta acggattgta tgttacatga aatttttatt gtgttcgttt tagaacaaga
    47521 agtgtcatgt catcatgctg tggcacatca cctctgaaat tgtcaacatc tttcactatt
    47581 aaatctgaaa gctctttggc attaagttgc ttattggcaa caactagatc ctttaatctt
    47641 ttttgaccat actcttcttt gtcagcattc ctcatttcag taacgccatc agtatacatt
    47701 attataatat caccaatatt taacaaaact ttagcttgct tatagtcaac atcttccata
    47761 ataccaattg gtacgccttc aagttttgtc aatgtgcatg catccagtga agaacgataa
    47821 caaaaaagtg ggccatggcc tgcatttgaa aacgacagct cattattaga acgattataa
    47881 atcataaaaa acagtgtggc aaatttatct attgcaaaat ctgcactgag tgaatcatta
    47941 attgcccgta caacatcagc acactgtaca tcccccctcc gtatatatga aacaaaaact
    48001 gttctgatca ttaccataac cagtgaagca ggaacaccct ttccggacac atcacttatt
    48061 aaagcaccca cccggtattc atctatgtca atgtaatcaa aataatcacc cccaacacct
    48121 tttgcagcac gtgtaaatcc ttttatttcg attccacttt tattataaaa acccattgga
    48181 ttgagccctt cttgaatttc acgagctaaa tcaatctgtt cttgcattat acggctttca
    48241 atttccacct gttgagcaac ttttaacctt tgagtcattt gattaaattc gttagcaagt
    48301 ttacctaatt catctgagct ttcaatttca attttatgat ctaaattacc accaccaata
    48361 ataacagctc cttcggtcaa tttcttgata ggctttataa taacacttgc aagaactatc
    48421 gaacctacaa ttgagataac aataaagaat gagccaatta ataggattgt aatagttaat
    48481 tttctgattt cattcaatat tatttcttca ctgaatccta atcgtacggt acctatcctt
    48541 accgtttgta ctgtatgaaa tagtggcaat gacagatcat aaaaattaac atttttttta
    48601 ttatctctgt ataccacaac cagcggcttt ccagaatcag tgttgttgga agccttactg
    48661 gttaaatcat catcaataat agtcccattc atttgaggat caaaattttg aattatacgt
    48721 ttattagtat ctattacata aacataatca ataaattcta aatttttaag atcctgcaca
    48781 gcgccaacta atggcaactc atctttctgg cttatggact cacgggctat gacagcaaga
    48841 cgttcaactt ccctgtttgc aaattgcaaa atttgtttcc gtaaaagttc agattcttgc
    48901 tgtataaaat agtacgtcat aacggtaata atactgctta ccagcaaaat aattgccaat
    48961 gaaaatttta gtcgcaggct aaatcgcact tcttttttag atgttgcaat tgtattactg
    49021 ttatcatctt taatattctt ttttttattt tttgatgttt tcttattttt ttcattatct
    49081 gaagcttttt cttttgacat catttacctt cctaaaagaa tcaagcattt tttaatatta
    49141 ttttttgctt tttcatgatc tggatctatg gcaagtactt ttctccattc ggtaattgcc
    49201 agatcaaact gattatttgt ataataatta atacccttaa aatagtattc tcttattaat
    49261 tcttgctgtc ggggtgttaa ttttttaccc tgaacctgtt ccgcaacctg gaatcgcaat
    49321 tgcgattcta ttttattaat aatgatagct acctgtatat tatcgggata tttattatta
    49381 ataatcttaa attctgctaa tgccttttcc agattatttt ttccaccctg ctgatataat
    49441 tgaagtgcca tattatatcg ttgcctgaaa cctatatcag caacctcacc acccgttttt
    49501 cttttttcaa cttttgattg tgttattaaa ttttgtattt caggatattg tggatcaata
    49561 gttgcaatta aatcaaattt tttaaatgcc tcattatact ttttatcagc taataatttt
    49621 tttccatcat taacaattgt ttcggcaaat tttttaaatg actcgggatt cttagctaaa
    49681 tcgcatctca ataaatattc agttgcgatt ctattctgcg gaaatagatt taatatcttc
    49741 acccattgct ctcttgactc tgcaatatta ccctgactat acagtctttt accagattct
    49801 attaatggtt gaacaattga gtaatagggg ttattttcat taattacttc ttcttcctca
    49861 acccgcagtg catccagcgc agtagaaaga taacgccttg caagctcatt ctttggatcc
    49921 aatgttaacg tggcttgaaa agcactgaca gcatctctat atctcccttc ggttaacgat
    49981 attaaacctt cctgaaaagt tttatcaatt tgctgtaatc taagcaatcg ttggcgttct
    50041 tcccactctt tcttcaattg tgagatagat tcaagcaatg cacgagcatc tttataatta
    50101 tcaatcagcg ctaatgcatt ttctagatta tcctgagcct gagtaaactc atacgttttt
    50161 agattgttaa ctccggcaat atagaactgc tctgcctgca aacgacgttg ctcgtaagta
    50221 cgtttttcat ctaatatatc ttcgattttt gcaatataat cttttgctac cctgtttttt
    50281 tcatcaagct ttaatacctg tacaaactca ttgcgtgaat caatatattt ttcctgttta
    50341 aagaattctt ctcctgtagc aatcagagcc ataatttttt caaattttaa cctgttgttt
    50401 attgctaatt cagcctggcg tttaccttca acagcaacag catcaagtgg cactaaacgt
    50461 aacatttcat cccatacttt gatagcctct tcatatcttt tttcattcat taatatttga
    50521 gcttcgctta ataaagcata atattttttc tttatttctt ctgataaacg tttttttgct
    50581 aattcaagtt taatttcatc actaagcaat ttaagttcat cctttaaaat ctgtagttcg
    50641 gggtcattag ggtctaagcg gtatgcaaca ataaaatgtt caatagctat gctggaatta
    50701 ttttttgcag cgtcaatatc ctccggtaat actttccgtg ataaccggta atataattta
    50761 gctttttgga atgaaatatc ctttttatgt ttttcttcat aaacaagttc aatattgcgc
    50821 tgggcatctt cattgttggg atcaatttcc aggattttta accattcaat aattgccttt
    50881 gtaagatctt ttttattata gtattctata gcctgtttat tataattatc tattagttgt
    50941 ttattctgga cttgtggctt gatagaagtt gctgttaaaa aagaaataac tattacaata
    51001 aaagctataa tgattttttc ttttttcacc tgtatgttaa aaatatttat cattggatat
    51061 tattgttgat gaataagcgt cctgtttgat aataaccagc ctttttcttt ttttgtcccg
    51121 gtggcatagc gaattaactt aatatcgcca ttcactacct gttcaccttt accgctctgg
    51181 cggtttatga ttgttgcaat aaactttaca tttttaaatt cgtaaggttt accatcacta
    51241 tctgtgccat tgtgcagttt gtcatccact tcaacaattt gtaaatttgt aatataataa
    51301 ttttgataca tagtcttaaa tgtattgcta tctttaggat aagcttctaa cttagtgtat
    51361 tctgcataat taagatacct gaccgaattt gcccatcggt tcaacagatt ttcaatatct
    51421 gagtaatccc tttggtttga acatgaaaac aacataaaag ataccagcat acataatgat
    51481 acaccaaaca ttttttttgt tatcatcaaa atcccccaaa aacaaaatag ttattactat
    51541 taactttatt actattaaca atatcgcaat tttaattcct tttcactgaa tacaattgca
    51601 gaaaattttt ctatttttgt attattccat ttccatgcat caggcggcaa tcctgcttta
    51661 taacatgtat gctccaaaaa tgttttaacg tcccatccat attcagttgc tacctgtggc
    51721 agcagtaacc ccgagcggaa tccactgctt ataataagcc catctcttcc aataactatc
    51781 tccgaaatat cattaactgg ttcaattggt gtaaggatgg aaatttcaat atcaatttta
    51841 tcaagttcac tttttgttaa aggttcaaaa cgcggatctt caaaagctga tgccttagcc
    51901 atgtcaacta ctgcttctgg tattgtctta attcctttaa catagcctat gcacccacgt
    51961 aaagcaccgt ttttatgcag tgtaacaaaa gcaccacatt tttgcttaaa aacttcatcg
    52021 ttaaattcat tgttgaaatc tacttttggc agcgttcttt tgtttaaata ctcttcaatg
    52081 gttttgcgcg ctaatgtaag caattttatc ttttgttcat cattgagtac tatcataatc
    52141 ttttccctta ttaaaaatat ttactttgat ggaggctttc atgagtatta tcaacagtta
    52201 ttgcatcctc cattatcatt gcacaaatgc agcagaaaga taaccaacca cctcattttt
    52261 aggacctgca gtatcaccgg aattagcata atgcagtatt tccacctttg tagccccaag
    52321 ttgttttgaa gcaatcatgc ccgccaatac cggaccttca ccgcaggctt gcgctttatc
    52381 gctccgcaag gcttcataca gatatagctc gtcaaatttt ctaagtgatt caataaaatg
    52441 tgaatcaatt ttttttgcaa tatcatagtg gtaataatgt gataaatccg ttgaaagaat
    52501 aatgacaaat cttcgctgtt catcatgtaa tattttagca acctcgctgc cgatttcttt
    52561 gcacgtggta aaatcaattg tccctataat aattggtact aaaacaaact gtggcaatac
    52621 cacctgtaaa aaaggaagct gaacctctaa tgaatgttcg tattgatgga cctcttttat
    52681 ttcctcaaat acattgtttt gtaaaagctt ctgggcgata gtttcgtcaa tctggacctc
    52741 tcctaatgga gtttgatata ttcctgatga aataactgaa gccccattaa aacgcactct
    52801 gtgtgatgga gccagtatta taacaacatc cacaggtttg tgtttaagct gtgcatatga
    52861 atatgctgca acaggaccag aatagatata acctgcatgg ggcgatatga atgctacggg
    52921 tttgtcaaga gaaacgattt ttgcatttgc aatatatcgt tgaatatcat tttttagttt
    52981 atcagggttt gctggataaa atgaacctgc gaatcccggc tgtcttatcc acatacagca
    53041 cctctccatg aaaattttaa catgaaatta aactcattta ctatttacaa cctataacca
    53101 ataaaaataa ttattgttat taatataata aaacacttct cagtttgcaa gcttatttac
    53161 aattaactat ctatataata actatctata taaaaaaatt gggatgcgct ggtctgcatc
    53221 cctttataat ggtggtatta attatattga tttattgatt tactttaata ttaaaatgtt
    53281 gcctgtaatc cagccagaaa atgaaagtcg ggcatgggat attcacaata cgagtcccca
    53341 agtggtggta ccaaaacagc aaaggtagaa tattctttat cggtgatatt atacaatgaa
    53401 ataaatgctg tatatttttg taaaaaagta tacgacatct ttgaatttat tataaagtag
    53461 ctgtcaaact cttttaaccc ttgcgtatca gcatagagct cattaacata ttcaccatca
    53521 agctgtatgg taaaatctgc tatggttagc gttattctcc cattagcttt atgcgccgga
    53581 acatagggta aatacccatt ttcctgcttg ttatcgatat atgaataacc tgcattgaat
    53641 gatagccatt ttaaagcctg tacagtaatc attgcttcag ccccattata ggaaaattca
    53701 tcagcatttt cccattgcga ggtatacaca aacatgttgt ccgaccatat tctataaccg
    53761 cccatatgta ttgtaattgt atcaccaagc ttttgctcca ttccaatttc agccatggta
    53821 tatgtttcag gtttaagatc cggattggga tttatggcag gatacatacc tttcagataa
    53881 acctgccgta tatcagggaa cttaaatccc cttgcaacac ttgaataaaa ttttgttttt
    53941 ttgaacggtc gcactgctgc accaccctgc catgcaatat aatctccata ttctgaatta
    54001 tagctgtaac gtgtaccacc gttaacactt aaaatatttt ttaaaaagtt ttgatccatc
    54061 aatacatata tcgatgtatc attcataaat ttatctttca tataatatga atcattttca
    54121 atatcctttg ctgttccgcc atatcgccgc cattctgcac cggttgtaac ggtattcccc
    54181 tgccatagtt taactatctc ctttaaccgt acagcgtatg aactgtcatc agattcaaat
    54241 ttatcaatat cagtattgct ctgctttgat tcatggttac caaaattacc ttctgcctgt
    54301 aatgttccac tatagttgcc ataatcatga gctaatttta caacaactcc acctcgtgta
    54361 atatcaaatt cttcaagaag gttaagctgg ttggttaacg gtatttctga taatgcatca
    54421 ataccttctg gacctggatc atgtatattc tgcttcatgc cgtagctatc cacacctgca
    54481 taccaattat gtgaaagcgc atacccaaca tgaacagtac cattctgctg cataaattca
    54541 gaagtaaatg ttttgccgtt aattgtctgt tcccaatctc catcagtttt ttggaactgg
    54601 tatccagcac tataatctaa ttttccaaat tttcccatat ggtacaagta atcctcggtg
    54661 gtattccatg ttccatacga gccctgaatt tcagtagtat atccatcctc tttttgtctt
    54721 tttgtgataa cattgatcac cccgcccatt gctcctgagc caaatagcac cggttgcgga
    54781 ccaatgagta cttcaatact atcaacattt ttcatgttca tgatgtcagc aaccggatgc
    54841 cccataatac ccatagttgt atccataccg ttgtatagaa taggtatacc tgttgttgga
    54901 ccccatcctg atttgccaat accacgcatt gacatcgttg cagcgccact tcgtgcaata
    54961 ccataaccca gcaccctgtt tgcaccggta taaaatgaag gtatttgatt ttttataaca
    55021 ctgattaaat cattattgga actatcctta accgtgtttc cttttattga ctgataagca
    55081 ccaggatata acaaggtatc ggatacaatg acatcctcca attgaacagc ttcgtcatca
    55141 gcctgtacgg aagctgtacc agataataca attgctacta taaatataaa taaacgcttc
    55201 ataagcacct tctacaataa tttatattaa taaagtgtta ctattattaa aaatatatta
    55261 tttgtcaata caaatattca tattattatg cagatataaa tatgcaatac cagagtgtta
    55321 taatgaagca ggacttatag tatttgtaaa aaggaaggct actctcatgt tttaccacgg
    55381 tcatccggca tcttgattca ttatctaacg ttaagaaata actgttaaaa caagtacagc
    55441 atgaccgtgg ttaacaattc tattttttaa gtatgtttac atgctcatat tgctttgaga
    55501 ctatgtttat tgcagtatct aaatcactgc gcttttgttc accttccaat cggtagagct
    55561 ctgacccttt attatccaga aaaagataca ccggcacacg ccacagatga taggttgata
    55621 catacatcgc gccatcagga ttattgatat caaccttttt tattatacta tcaggataat
    55681 gtgtctgcac atattgaata gtatcatccg tcattgagca tgattcacaa tttttagcgc
    55741 tgacaatgac aatcgttatt gaattgtttg tattgtgcga gacagtacat ccatgaaggg
    55801 taattatcgc ccagaaaaaa attaatgaga tacaacatgc taaggatgaa attgttatcc
    55861 gactattgga caccaatgtc acctgtattt tccttttcta aaaactcaat acgcttgcgc
    55921 ttcattgcat cttcaaattc cttgtccgat agtccctgta aagagtgtaa ccgtgtttta
    55981 tattcgccat catacggttt catcatctcc ctgtccatga attctggatc aggtgtggtt
    56041 gcttcaataa agcgatagcc acgcaaatcc ttcagtgtca atggcggctt tgcctttttg
    56101 tccagcaaca ccttgccaaa gaaaagaagc ggtaccattt gcttcccgct ctgaagctcc
    56161 tgtttttgca ccgtataggc aacgggttca ccctctgcat caaaaagatt tgcatcaaca
    56221 atatagtatc ctttccgtag cacatcaatt tcaacatcga tgtacaatga accgtcagca
    56281 atcctttctt taaagctacc ggtaaacttt gcggggatgg tgttttcagg gaaatattca
    56341 aatggtattg ctgcctctac atgttcattg ccagcattaa actcaacctc tgcccagtat
    56401 gcggatgatt ttttttctga tgaaaaactt tgcgagcatg tcagctcagc agtatagtca
    56461 ccagttgtat tttttgcaag cgatagttct gcataatatt ttgccttgtt tttattaagt
    56521 ccctttttta taacagattt tgttatagct attggaattc gttctgaatc aggtttagta
    56581 ccccgcaaca caatcaatac aaatgtaatg gtatcatcgc caacaactcg atacttatca
    56641 gcagtaaaga gatagtagat atcactgccc tcttcaatag gcaatggctg ttcataccgg
    56701 cggttaggca taagcaggtc aatattctcc ttgctcaatg gcctgctgtt aggaggatat
    56761 atcgatagct gtttatagtc ctgtagagct tgctgcggtg atatgagtcc catctcttca
    56821 agcaccgtac tatatccatg agaactttcg cctgctgcct cttcagcgat ggtatcgttt
    56881 ttattagcat agtgtgttat atacagcata ataccagcaa taataacaat gattattaat
    56941 gaaatatgag ttaatttaac tttcatatat aaaaactccc cttatgaaaa tatcataccg
    57001 ctgtgtcatt ctaaagggac gtagtaccga atattctatt cgttcataaa acgacacgat
    57061 tcttcgctat cgctcagaat gacatgttac ctcaaggtca ttcccggctt gaaccggaat
    57121 ccacataatc aacgttagat actgaaatga gcttattgaa ttataaatac atacattgaa
    57181 tacagtctac tacaaagaat ttctattatt agtaaatcaa taaaaaaagc gcggtattac
    57241 aaaaccgcgc atatattaaa agattaacta aagtgtttac catcccagtt gagtgataaa
    57301 tttcatcttc atttcataat gatttaaata atatcccgag catgtatagg ctataacgtg
    57361 atttgtccag tgcgtacagt tgcacagatt gttatagctt cccgaattga cacaagcagc
    57421 agatgaatga taggcaacag caccatcgtc ctcaccaggt agtaaagccg aagcaccaaa
    57481 aatgccttta tatccgccaa tatggtaaat gattatcccg tttgtatcat tatggttata
    57541 catattgcgt atagtactta cacccaactg cccccctaga ttgcaggtaa aaagatcaga
    57601 aattcctcta attgatattt cactgccgcc acctgcacct gctgtagtat agacatacgc
    57661 aatattccac tgcggtgtgt aattggcatt gatatacccc acgattgcat cgccagcact
    57721 ataattaaca acataacacc agttgctacc tttgcagtaa acatccagat aatttgtttt
    57781 aaattgactt gctgaatatg caatggtgtt gttgttatca acataacaat taacagatac
    57841 ataccccggt gcatttgcta aacgggattc tccaccttta ctcttgccat ccagccaatg
    57901 agttgagcac attccattga gatgaagaac atatgttgta ctaaatcctg ccgatgctac
    57961 aaacataaac ccaattaaca gtattaaaaa agcaaacctc ttcataagcc ccccatatct
    58021 atactctata ataaatatct accgaacaga cggtatctaa gagacaatat agaaaccttt
    58081 aaggaaaagt caagaaaaaa attacaatag taaatattaa tcaatatcaa tttatttatt
    58141 agcaggaagt taatacacca tctctataac attacaatgt tattttgcta aaaattatgc
    58201 attgtttttc attaccactc ctgatacact acctcagcaa aacccagcat atcatcaata
    58261 tgaagattgg tattatcatc aagaataata taaccggaat aatagccaga tactttatta
    58321 cgatactgct ttgtagtact gatttgatat attgctggta ttgaaaatat tttccataat
    58381 tgttttagaa aactgaaagt ttctgtaagg gttatattat tattacaatt tacagttgac
    58441 gtgctgtata cggttatgca ttatattaat aattaattga aaggcaagtt catgatatgc
    58501 ttaaaaaaat tatcactatt ttgcttatac cgtttctgtt acaatgtacc tcaaaaagtt
    58561 atagcattac aaaacaatca aaattagcag gcacatggta tcctgacaat gctcagcaac
    58621 taagtcaatt aatttccagc cttaccagaa atgccaaaca ccatgccata aaccgcacac
    58681 caatgattat agtccctcac gcaggatata cctatagtgc atatgtagcg tcaaaagctt
    58741 atggggcgat agttacttat aaaccagata tcatcattat aatagggcca tctcattatg
    58801 aacagtttac cggttttgcc ctgcccgttt atgatgccat tgccacgcca ctgggaacga
    58861 tacctgtgga taaaaaaacc gtacaaaaac tatcagccaa taacaatttt gtgtttaaca
    58921 acaaagcatt tgaacctgaa cacagcgttg aaattcaatt gccatttatt caatatctat
    58981 tcccgggaat ccctgttata ccaatcctga caggcagcac aacccctgat acagcaaagg
    59041 aagctggatg ccacttacta tatgcgatag ctcattataa aaagcccttg ttcattatca
    59101 gcagcgactt tactcatcat ggtccgcgtt tcttttttac tccgtgtagc aatagtaata
    59161 aaagcaaaca gatgcaatgc ataaaacaag gtgatcaaca ggcaattgat tatattttgt
    59221 catgcgatgc tgataaattg tatgattact gcaaaaaaac cggcataacg atatgcgggg
    59281 taaatgctct tatatttgga ttatccatat atgaaaaggt aaccacaaaa caattagcag
    59341 gttatgcaac atctgccgat atcaccgggg attttgacaa taccgtaagc tatgctgcaa
    59401 ttgttattga cggtatgctc aatacacagg ctaaaccata tgcaaacaac caaccatcaa
    59461 tgctttcaga caatgataaa gcttttttgc tatcattagc ccgacaatcc atacaatcat
    59521 atgtattgag caagaagcct tttcactaca gtggcaccat accagaagca tgcttgcaca
    59581 atgccggtgc atttgtaaca atcaccatac agggaaatct gcgcgggtgc attggttaca
    59641 ttgaaccaat aaagcctctg tataaaacgg tgattgaatg tgccgccagt gcagctatca
    59701 gtgacccccg ttttgagcca ttgtcacctg aagaatacag cacaatacgt attgagatat
    59761 cggtattatc gccgcttaaa aaaatatcat cgcttgatga attaacaccg ggaaaggatg
    59821 ggcttgtagt tgtgaaaggg aacaggcgtg gcgtactatt gccgcaggtt gcacaagaat
    59881 ttaactttac aaaggaggaa ttttttcagc aaacatgtaa caaggcagga gtatatccat
    59941 ttgaatatga ttcgattatc atgtatacat ttaccgctac cgtctttcat gaacgtacac
    60001 cttccccata atgaaggatt gatatgagca gattaacacg gaaagatatt ttacaggcag
    60061 gtattgcttc actgcaaaca gcttttttta caggaactat cgccccatta tatcattgtt
    60121 ttgccgccat tagctatcac gaagcaatgc actggaaaaa ggttgataca aaaactgtac
    60181 agtgcatgtt gtgccccaat caatgtatgt tgcgtaacgg tgacaaaggt gcatgcaagg
    60241 cacgtcaatg tattaatggc acattgtaca gccttgtgta tgggaacatt gccgcttacc
    60301 atattgaccc catagaaaaa aaacctctgt atcattttat gccgggtaat gaagcgcttt
    60361 cagtagcaac cgccgggtgc aattttagct gcaaattttg tcaaaactgg cagctatcac
    60421 aaagctatcc tcatgagctt gacagcaaac acataacgcc agttcaactg gtaaacaacg
    60481 ccttaaacaa aaaaacgccc attattgcat ttacctacaa tgagccaact attcaatatg
    60541 aatacatcta tgaaacagca gtgcaggcaa aaaagaagca tgtagcctgt gttattattt
    60601 ctaatggata ttgcaacaaa aatgccagtg tactattgta tccaatgctt gatgctataa
    60661 agattgattt taaggcatat tcacaaaaat tttacctgga aatttgcggc ggaaatttac
    60721 agccggtttt aaacaacatt atactggcaa aaaagaaaaa tgtgtggatt gaacttgtta
    60781 atttagtaat cccaacgctc aatgataatc ccaatgacat taaggctatg gcacgtttta
    60841 taaaatccca tgttggcgat acaacaccat tacactttac acgcttttat cccatttacc
    60901 aattaaaaaa tatcccacca acacctgttt caacacttga gcgctgcaaa gaaattgcag
    60961 tttccgaagg acttaaatat gtatatattg gcaatgtgcc cggacaccct gctaacaaca
    61021 cctactgcca tgcatgtgga aaacttgtta tagaacgcag cggattttac actacctcca
    61081 ataagctcaa atcaggacac tgtccgcatt gcaataccag aataccggga atatggacat
    61141 catgaaaaaa aaggtgctat ttgtaattct ggcagcattc tttataggct tttcttcaag
    61201 catagcgcag gcaatcatga tacgggaact tttgacgata ttccggggca acgagctgtc
    61261 ggttgggatt atcatggcaa tatggcttgg cggaatattc accggtgcag tatttagtaa
    61321 aacaaaacag attgttcatc caaaattcat catagcaatg attattgttc atcctttttt
    61381 gtttttagtt tcattaattg tactatatag cataccgatt gtatacagct catacggtgc
    61441 cttttaccag cttgatgctg agatcatgat tgctttgcta tgccttttcc cgcaatgttt
    61501 tgtcattggc tatgtttttc ctgtattgat tcagtatgca gacgagttat tgataaaaca
    61561 tccgggaagc attgtctttt tgctggaatc atcaggtgca tttgctggtg gcattgtatt
    61621 tacatttttt atactgtttg ttttaaatcc cattgggaca gctatagtac tgctgctctg
    61681 cgccaccata ctgtcactac ttatatataa tcaaaaaaaa cactatgtca caataacact
    61741 ggtttgtatt attcttttgt ttttaacaac tcaaacaaat aaagcagagc aagccctgat
    61801 tcaaaaatca tttgcaataa atcacccggg ttcattgctg ctctataaaa gaacaccata
    61861 tcagatgatg tatgttgcaa aaaattatga tacgtactca ctctatggca acaatatttt
    61921 ctatacacag cttcctgatg attatgaggt aaggccatta ttgcatgcac tgtttgcttc
    61981 aatacatgat attttgggca aaaatatact tattgccggc gatacattgt atcttcccta
    62041 tgttgctaca aaattacagg catcgattga ttattgtacc gttgacagat cattatggaa
    62101 caccttccat acacttggca aacaactata tccaaacttt gattttaaca aacttaatgt
    62161 tatattcagt gacacacgtg gcttgctgtc aaaaacacca agaagatggg atggaattgt
    62221 cattatccct ccacctcccg atagcatact tcacaacaga ttatatacat tagaattttt
    62281 tcaattttgc agaatgcatc ttaaccagaa tggtgtattc atcatgcaga tagaggggtt
    62341 tgccaatatt atgaatcctt cgttgcaacg ctatatctca tcagttttga acagctttac
    62401 aaaaatgttc ccaacatcca tagtttcagc cggcgaaacc atctatttaa ttgggtttaa
    62461 taccggcaat accattgatt tcaataacta tcgccagaat taccttgcca attttttaaa
    62521 atctgatacc ggaaaatccg ttgcagcact aatccctcat tttaatccaa aagaactgga
    62581 tatgtttttt cagtcaactc accttgccta tcttaacact gcaatcaaac actctgcaat
    62641 gttaaatacc gatattatgc cgcaagcata ttttgaatat ttagctaaca cgcttaagca
    62701 aactgattca tatacaggca atttcataat gcaaccagca gttatggcaa tcataatgat
    62761 ttgtattgtg gttgctgcta ctgtctattt tttacgacgg ggtaaagagc acgcagtaaa
    62821 agggctggtg gtatttattg caggttttat cagtatgtcg gctatggtta ttgcacttat
    62881 ggcttaccag aatgtatacg gaattcttta ttacaggata gcattttgta atgcgctctt
    62941 tatgcttggt ttggcttctg gttgtacaac aacatttgct gcaaagccac tatatgcaat
    63001 gcggatagtg atactcatcg gtagtttggc aatgcttgct cttttcatca tcatgaagca
    63061 tgtatggttg ctgtattgtg ctattttttt catagcagca atcacaggct ctttgctacc
    63121 attactgtta aaaaatacgc aggaaacata tacaaaacga ttgctggctt ctacactgga
    63181 tgccagtgat caccttggtg caatgtgcgg agctttagct gcaccgttta ttgtactccc
    63241 tatttttggg atgtactcca ttggcatctt actactgctt tcaataagcc tgtttatatt
    63301 caaaaaatgg atatagaaaa attgtataca tcatcaacta ttatattaca aaatttggct
    63361 cattttttac agtagcatac cattcatcca tcttctgtat ctgttctttg cttccacgaa
    63421 ccaaaaccca gtaactgccc tcggcaccgc ctactccacc tgctgcaaaa agtttagcct
    63481 gtgcgccaat aaccagatgt atggcatcaa gctctgtgat aatattccct gtaacaggaa
    63541 aataccgtac accctcggtt gtagtggcat taatcatttg agacaactcg tttatattat
    63601 catgcagtcg cttttccaat cctaccggaa tataaagctg tactcttttc cctatgacaa
    63661 cctgcataat tgcaccggta gtaccagcct ggggatgccc tattaacact gcagcctgct
    63721 gcagctctat attgactgca tttgctcctt tgatgattat gtcacctttt tgcaggtcac
    63781 ccataacatc aaatattgtt ttattttttt catatatacc acctttaatg acgacatcac
    63841 ctttaaatgt cgattcatcg ggcaatcgcc ctgattcagt gcgcttatac catgacggca
    63901 atgttattcc tctaaaaaaa tgttgcattg aaaatccttc tgactgattg acatggcgca
    63961 gcatctcttc agcaatatat ccattggttg taccggctac gataacaata gttttgctgc
    64021 gcagggcttc tataatctct ggaatataca gcattgattg tgcaatcatc ctttttccag
    64081 cagcaggtgt aataaggtat tgtctggcaa tcatagttac ctcactatca agaatatatt
    64141 caataaaaag atcatggtat aatctataca aaaagcaaac aaaactacaa agcttgtgga
    64201 gacggctatc atcataaaga tgttagctat acttatcatg aagatttccc tgtagtataa
    64261 cgctgccata tataataaac gggaatgccc agtagtagta caccaatagc aaaagctgag
    64321 gtaatggtcc agcagcagag cgctgcaatc atcatgatga tggtcaatac aacatataca
    64381 aggggaacaa gggggtatag cggtgttctg tacgagcttt tgagctcggg atgttttttc
    64441 cgaataacca tgagccctat gacggttaac acagggaata cattgagcgc aaaacccatg
    64501 tagataacca gcgacatagc cgttcctgaa tatacataga taaccgccag aatcatctgt
    64561 aaaacaatag catagagcgg agtaccaaaa cggttgctga tttttgacaa caatgaaaag
    64621 atcatgttat tgttagccat agcataatac acacgtggcc ctagcatcat ctgtgcagat
    64681 actgacgata aaagtataat ggcgatagtt atactaaaaa aattgcttgc aacaggacca
    64741 aataatgcat tggctgcaat agcacctatc tcatcatgat tcatgatatt aatgccatcg
    64801 gttgacataa gaaaaacaat atttaaaagg atatacaaaa ctgtggtgat agttactccc
    64861 cagaacatta tccgcggcaa atttttttca ggagctacta tctcacctgc aatataggta
    64921 gcaccattcc atcctgtgta ggcaaacatc accattaaaa gcgatagtcc tatcaatggg
    64981 attgaacctg cttccacggt gccatagtgc gcatagagcc tgtgaatagc ggaactatcg
    65041 agcacataaa accctgcaat aataaatgcc gcaacgatag caatttttat gacagtaagg
    65101 atattctgta tggcgcttcc ccgttttacc cccattatat gaatacagcc aaatgttatg
    65161 atgataagcg cagcaatatt tttttgccag aagatatcat cggtaatagt ggttatacca
    65221 gtagcatgaa tgtttataaa aaatttattg agatactgca ctaaaagcat ggagctggtt
    65281 gcaaccggtg cagtaaatcc aacaatgagt gatacccacc ccgttaaaaa tgcagggagg
    65341 ttgccaaata ttttctttaa ataaatatat tcaccgccaa catcaggcca tagtacagct
    65401 aattcactgt agcttaatgc gccacacaga gcaacaacac cacctaacac ccacaatagt
    65461 ataacaatca gagcgttgtt ggtcatgccc aatatattgc ctgttgtcat aaaaatcccg
    65521 gtgccaatca tatcggcaat gataaccatt ataccggtaa agagtcctaa ctggcgttta
    65581 agctccaaaa aattacctct tattttgaca tgacatacgc agtttctaat ttaaccgaaa
    65641 gcacctttga aactccgggc tcttccattg ttacaccaaa aatagaatcg catacactca
    65701 ttgttttttt gttatgagta acaataacaa actgagtgga ttttgaaaat ttctgaacaa
    65761 gccgtataaa acggcctata ttctcttcat caagtgcagc gtcaatctca tccaggaagc
    65821 aaaagggcga agcttttacc atatatgttg caaacaaaag cgcaatcgct atcatggagc
    65881 gttcgccgcc tgatagcata tttatatttt tattcttttt cccgggtgga cgcactataa
    65941 tctcaatacc actttcaaga acattttcag gatcggttaa ctcaataatt gcttcgccac
    66001 cattaaaaag ctgcttaaat atctcctgaa aatttttttg aatttcttta aaagtctgta
    66061 aaaacagatc tacggattgt ttattgatgt cagcaataac tgacagaata tcttcacggg
    66121 ctttctctat atcttttttt tgttcaatat aatattcaaa tcgttttttt aagtccttat
    66181 attcttctat tgctaagttg ttaataggtc caagctcctg gatttgctgt tttatatttt
    66241 gcagttcgct attaatgctt tcatacaatg attcatcaag ctttatgctg gtatcagtta
    66301 ggcgtgtttc atattcagtc cataaatatt cttcaattga atttaatcta aactgcaatt
    66361 caacaatatt tttatccaga gagcttatcc tgtcggcaag ccgttgcagt tgttccatgt
    66421 ctttttttga tgattgctta cgtccaataa tttttttttc tatttcatca cgttttacaa
    66481 tcagatcatg aatctgctgt ttcaaatcat tgctcttttc attaaattca accatgctat
    66541 tctgccattg ctctatctct ttcaccagtt cgctgatata gtgttgcaac tttttaattt
    66601 cattttccag gtgtaccttt tgctttacat catcggctat ctgcctttca atagtctgaa
    66661 tgtgtttatc aatccatgct ttttcatttt cattcctgga taaatccttc tcaacacggg
    66721 taatcatatt attaacttcg tcaagctctt cctgctcact gtgtatgctt aattccaatt
    66781 gattgatatc attccgtaac gtttctatat cctcagtagt tttaaggatt gctttatcaa
    66841 gcctttcttt ttcggcgtgt atacctgtct tatcaaacag tatgcttcta aatccgtctt
    66901 cataattttc aaaaagctgt atatcctgct tcaatacatc aaatttaatt ttttttaact
    66961 gctgtatggc ttcgtcaacc agattcaaag ataaattatg caatgcattc tgcaaacatg
    67021 tttctgcttc ggcaagctcg ctgtgaattt gctccctcac ctgctcacgc tggctttcag
    67081 atgattctaa ttcggattta cgtttttcaa tagcatcaac cagttgttta ataactatct
    67141 ccaatgactc cctcaatttt tttacggtta tctcaaaatc cttaatggca tgtttttttt
    67201 gttcgatagc atctctaaat gaatgtatat tctgaatttt ctttttccgg tgagtataaa
    67261 ggttttcaag ctgctgttta tcgctggcaa tattgttaac gattatctgg ctttgctgta
    67321 tattcttttc tttttcttca gtcaattttt ttacagtttc atcaatttca ttgatgcgct
    67381 gtctggtagc agtaatttgc tgttcctgct gttgaataag ctgtttattc cgtgaagttt
    67441 tcccatctat atcttcaagt tttatagtat aggtatgcag ctttttatcc cattcaaaga
    67501 gctgcaactg aatatcattt tttctttttt catcattttc attttctgat gatataaggg
    67561 taaccttctt tgaaagctct tcgcgttcgg caaaaagttt ttccctgtct tcggttacct
    67621 tctgcaattt tttttgaaaa tccctgtatc gcaataattg cagggaaata tcattccttt
    67681 tatatttctc gcgcaagcca aaatagattt ttgttttttc agcctgtttt gattttaaat
    67741 ctttttcgcg ttcaacttct ttaataatat cgtttaaccg tgctaaatta accgtagtgt
    67801 cctcaagctt ttttaacgac tcttttttct gaaccttaaa tcgcgaaatg cccgcagcct
    67861 cttcaaaaat atagcgacgg tcttcagctt ttgttgaaag aattaaatct attttgccct
    67921 gctccataac agaatatgaa gctttaccaa gtccggtatc aagaaaaagc ttttcaatat
    67981 ctttaagcct gacaggtgat ttatttatca tatattcgga ttcaccatcg cggaaaaccc
    68041 tgcgggtgac agttactgtg tcagaatcaa tatgtaatat cctgtttgtg ttgttaataa
    68101 ccagtgaaac ctcagccagt gacaacggtt tgcgctgatc ggtaccggca aagataatat
    68161 cttccatttt atcgccgcgg atattctttg cctgtttttc gcccagcacc catttggttg
    68221 catcaacaat atttgattta ccacaaccgt ttggaccaac gattgcggtt atgccttttt
    68281 cgaaatgtat ctttatttta tcggcaaatg atttaaatcc aaatatttcc agtgagtcta
    68341 aatacattga taccacctta caatggttat agcattatgc atgattatag tgcagtaatt
    68401 tctttacgat tatctacggg aatctttaaa aaaactctgg ttaacatggt aaaaaatcag
    68461 gaaaaactgt ttttaccact atagtgtcat aaaacagcat taatcattgc cattgaaaac
    68521 tttttccaat tttctcatgg tcatcgttaa aaatcagggt tttcgtaatt atcaattatt
    68581 atcaatataa acaaacatat ctccaatacg tatgccacaa aaaaatatcc ggaattgaat
    68641 tatcagggtt atccttatgc cgcactataa aaagcaatta catgcaacat accaataatt
    68701 gcgcaacaac attttttaaa aaaagattta cccagacctg gctatgttga ggaaaataat
    68761 tgttgcaata ataaagtaat agtattatca tcgcagtaac aggatgatga gcaccatcaa
    68821 acaacaatcc ttatgtttaa ttggcacaca taacggcaga aactataaaa atagtattga
    68881 cctatatgtt atgtattatc atcaaggagt tgctgatggc tgcgctgtag ccgctcaata
    68941 agagatggaa atggattgtt accgttgttc tgaaccgtag aaaaccatcc tgaaccgtag
    69001 gacgtcatcc tgaaccccga ttcaggatct aacagtgatg aataattatt tttttaggaa
    69061 gttccattgt aaacgagaag tagtatagta ataattacca ttaataaaat tacggcgaaa
    69121 atgaagatga aaaattgtaa taacattatg atccatattg ccattgttat gtgcgctgtg
    69181 atgcttgtca tagcatgcga tagggttaat cttcacgaaa tagccgatag tgactcgcgt
    69241 acagcatatg ctattgctta taatggctcg gtatataagc tcatcatagc aaatatatat
    69301 tttgataaaa aatttaccag ctctactgtt ttaccaaacc ccatgggagg tattgcagta
    69361 aacaaagaaa aggatattat ggtttacaac aatacaacca tgcttgcact atcaactcct
    69421 gatttaaaaa attggattac ttcaggtgcc tatccattat ataatactgt tttaggttat
    69481 catgatttct ttttatattt taatgaaatc aaccaacaaa ttaatcaatt tgatgaagat
    69541 actatgagct ggaatgtatt tgccacagca tcaagcaatt cattagggat gtttcaggct
    69601 aacaattcac aaatatatat tgttgaaccc agcggaacaa atctactttt ttatactctc
    69661 gataatccaa gtacgccgtt tttaacagtt gatataggtt ttacacttgt aaatcctttt
    69721 gggggttatg caaataacaa cattttttat atctggagat ataatccaac aaattcaatt
    69781 tttaaaattt actccgggac ttataacgtt cttaatcctt ccattgctgt tggtgatata
    69841 ttaactgacg taaccgtaac tgatgacgat aaagtctttg ctatagtatc tgaatcggga
    69901 aatgtgtatt taaaacaaat tatttcggat ggcaattatc catccttata taattttggc
    69961 acaaccggga catttactat tgatacactt gatagcactc atcttattat tgcatcctat
    70021 ggtaatactg aaagttacaa tggtttattt atctataata taaaagatag aaaaatagaa
    70081 aaaattatca ctaccgaaga tgttattgcg ttgtgtgttc cgcgctaaat tttttatttc
    70141 atgtacaggt aaaacgcttc tatccataag aatgaaaatg tggtatcatg tcatcctgaa
    70201 ccgtagaacg tcatcctgaa ccccgattca ggatctatct aatagtatat attgagattc
    70261 cgggtcaagc ccggaatgac agtgtggtaa agagattccg gaacgggaac ggaatgacat
    70321 tgtcgccata attttattga tggtaaaagt tttccctctc gaatattagt ttactaaatt
    70381 aaaaaatagc ttgaacatat gtttaatcca attcattatt atactcatgg gtttattaat
    70441 aatcatttta ctttttattc caggatgaac aatgaaacgt agtaaaatgt tatactgttg
    70501 tttgttgata catgtaccag tatttgtatc ctgtttatcc gtaccgtcat cacagcatga
    70561 taaaatgaaa acatttacct tctcatctga cacggtaaaa cttacgaaaa gcaaagaact
    70621 cattgacaat actgcaacct atgagttgtt gtttgcctta tccgaaaatg ctcaatgtgt
    70681 ggagctatcg gtttccacac aggatagaaa cggcaggaag gtgcgctgtg cttatatcgt
    70741 ggaatcattg gttgatgttt ctaaatatgc aaaaaaacct gctaagccca tctatgctga
    70801 aattgcaaaa aattttgata tcaactggcg tttccagagt aagatatcaa tttgcaacga
    70861 taaaactgat ccattgaaaa gactcaataa gggtaactat cgcctcagaa tttcagcatt
    70921 taccaatgat ttttatattt ttaccgttga tataacatca ccggttgaaa ttttatttat
    70981 acaataacta tcgcacaaat atgccaaaac actatgatga catagaatcc gaagacctgt
    71041 atgaaatggc catgcaatgg gctgcgcagg gtttttttga aaaagctatt gagcttataa
    71101 aacggtcaat agaattaaat ccacatttta ttggtgcata cattgatctg gcaaaccttt
    71161 atgcaaaaaa gcatcaatac caggatgctt tccatatatt aaagaaggca tcaaaaattg
    71221 atacaa
//
LOCUS       DAOOJH010000003        67113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_483_length_67113_cov_10.570924, whole genome shotgun sequence.
ACCESSION   DAOOJH010000003 DAOOJH010000000
VERSION     DAOOJH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 67113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 67113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67113
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_483_length_67113_cov_10.570924"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..425
                     /gene="hypA"
                     /locus_tag="PLJ75_00680"
     CDS             72..425
                     /gene="hypA"
                     /locus_tag="PLJ75_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPD03766.1"
                     /translation="MHELSIMSNVLDIVVQHAQQNGAVKVTKIHLLIGELSDYIPEWM
                     QNYFDFVSKDTIAENAQLVVEMVKASLQCGDCGTAFAFNKDDWQFSCPKCHSPNVTIV
                     SGRELTVKTIEIEGA"
     gene            828..2279
                     /locus_tag="PLJ75_00685"
     CDS             828..2279
                     /locus_tag="PLJ75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPD03767.1"
                     /translation="MLKITFIIKHGMAVLLCLCIFQCIGYAGPIVKIEHVDITSFPIV
                     SISGKLHAIQRRNSITKDNFTVLENGKVIKELSLKHGQLDNVYIALCIDTSKSLSKNN
                     FLRLKNSAISIIKKSPDYYKFLLYKFNNEVVQLASHDDDSMVHVAKIKNLQQLGKCTQ
                     LFNCLYTAIDDLSKFDGVKAILLFTDGKDEGSDMLPDDVISIAREHSTPVYTVLPQGC
                     RKTAVVSRLSKAANGKLFIISDDINKKILQLMQSRISSGIIPFELSYNSSSTDGIGSV
                     EVRFNNGTVSDNDIQYINFKAPSFTAYRFNNVPFVVLAGIIVLLIVALVIVFIIFMNK
                     WKHIIELVSMQRIPRPVQHYYDVLFEKDEAGRKEESNLLLSSSPEYSYTSAWLVEKSG
                     PETGKKFPLIWDEVTIGHGKENTIVINDDAVSLKHAKIKKINDCYYLFDCASDNGTFL
                     NNKKLLRPKMLTDWDEIQIGRTTILFRGTKIKH"
     gene            2284..3798
                     /locus_tag="PLJ75_00690"
     CDS             2284..3798
                     /locus_tag="PLJ75_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPD03768.1"
                     /translation="MPLKVLLCSPPVFDFYYSFHRSEPLGLLYIKAMLQQYTWLDVDI
                     YDARQKAKSKKLQTPDVFQYLNHIYMHDTSWFSLFCGYKRFGSSFDRIVGLINEKKYD
                     VVCISSLFTAYHHDVELLVKKIKEHSSAIVIVGGWAVWSMSTVEKGYADVYVKDDGEL
                     VLPELLHTIHTSNNKCMSKLPKIIKSSNGRSNEFFSNNFPERCGWYTYYGKRIANVIC
                     SKGCIYQCAFCSIHSRYTYQRRAFQSIEEEFHYLSTNGVQIINFEDDNFLYTRDYALQ
                     LLSLMKRYKEKGMNYLCMNGITASNLHAVLDDAMESGFLEFNLSLVSANDVIARRYNR
                     PLIKEMIQDIAFRCAGRVKVIVYVIAGLPGSSIDDCIQDIVLLAKLPVIIGFSPLYMI
                     PRIPMFETMGLPEDMRLCRGSALYPFGNDITREDIASLWKLCRFINKMKYLPHNPDDI
                     HLLPEHIYYYTRTCREKRWYYKSSDGTWQQGFSFQASLPEHIHVCDINGNAREM"
     gene            complement(3819..4502)
                     /locus_tag="PLJ75_00695"
     CDS             complement(3819..4502)
                     /locus_tag="PLJ75_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD03769.1"
                     /translation="MFKPPLTLQTTTLWNYPSQNYGNQKQGDQNYPGATPSYVIWNLL
                     MRYTNKGDMVVDPMCGSGTTIDVCNDLHRKCLGYDIHPYRADIMKADARNLPCKSNSA
                     DFIFIDPPYGDNISYSNEEQCIGKLSAKSQEYFDAMAIVIKEIHRICKPKHSMGLYIC
                     DYYNKKDGFIPIGFKLFSILLQYFKPVDIIAVARHNKTLTMGNYHRAAEEGNFFLRGF
                     NYCFIMYKE"
     gene            4649..5197
                     /locus_tag="PLJ75_00700"
     CDS             4649..5197
                     /locus_tag="PLJ75_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD03770.1"
                     /translation="MKKDTHECVSKCYVLYGNLVKKIIAGYFTSNEDIEDIFHDVFLK
                     LVRVDLKGDPFDPAIKHYIVKATRNTCISRIRKEMRHHNNGDSYWLSQIATIEEVNNK
                     DMAETMIDGMVVNTLYDVIKELSNDEQSLLYKKFYQNKKFSEIAAECKSTYYFIDKQL
                     KTIYKKLSLKMVRTCIDHNDIS"
     gene            5294..7450
                     /locus_tag="PLJ75_00705"
     CDS             5294..7450
                     /locus_tag="PLJ75_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD03771.1"
                     /translation="MNYTIITICAITVIFALFVAIINIVKRYSYAVNRLFSLIAILTA
                     GMVSCLIIPYFIPDIDIVQLTRWYFGLIIIINQGFFHYTQIFPRWEKKSPLWMIITTA
                     IPGFITFVLCVATDTIITHASFIEYYFNFSYGVLIEVYLVVFIFYIMGTFFTLFYKVR
                     RLENTSFRYQLFYIFMGNHFGALIILITFIILPYAFKIYDFHSPGIASAALILLLINN
                     YAISDERYLDVKEFYFKIVFYCIVLAMTVIPSYFILVYSYRFFLRGGQIPAVAVAFIN
                     VIYLFVFTRFIQPYLEKSFKREYVRLETRFNTFIQALSQAEEFEGDPAYWDKFFSTTI
                     DSLENLFNVSSASLLMYNESEKAFILSHSIGEQTKIKRIVSTDSIVQLCEMVTTTIHQ
                     SLFYTDDTIKAFDDVLILVKSNNIEVILPIFDANGKLVAILLVGPHKKGRPYTYNEIQ
                     FMELYRVKFGNALVNSLQIEDVKTKQVAEHDQLVVNAIKTSMQPKQIPFIDHIRISSI
                     LMNNSNLGGEFCNARVLRKNLLGFLIADCSYSGVDAAVLALELYGAFESCSPVYDAPE
                     KIMQLLNWVVCTSRFTDRYATAFCFTFNSDDLSLKYLNAAFNPLILYEIRSDSFLELD
                     TKGIPIGIERDFIYESKEYRANGPCIGCIYSNGIAGAVNADGQTYSIGRIKDLIRLHN
                     SETPALIIRRISSDLNDFISNTALKEDISIVIFRIE"
     gene            7480..8589
                     /locus_tag="PLJ75_00710"
     CDS             7480..8589
                     /locus_tag="PLJ75_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPD03772.1"
                     /translation="MNIDFKESAHKIISILQVQKHIAIFIKGSPDPDAIASSYFLYCL
                     CDALHVKATMFAFMPASLPSNREIISLFSIPLYIEDSIKAINLQRYSGYIVCDFQTAL
                     IDGIINAIPCLIHLDHHAPVEDDVPAHFRILTDEVSSTSTLCALIMQYISCFSQDMIH
                     LLATVLYLGIKTDSDNFNHLNKYDVMAIEYLKQLYIPSMIEKIESVPLSLYSQRLLQH
                     AMANVVIYKDWLIAGVGFVEEEHRDTIAIIADYLLENNHVELAVVYGAVYHNNHTKLD
                     IDASLRTKNEDLDLNELIKNITTDGGGRKYKGAFQVHIDYFVYCPDQERLWDIIALTT
                     HHILKNIRDGIYKITIQNKFKKLKKTLKKLFIKEK"
     gene            complement(8593..14226)
                     /locus_tag="PLJ75_00715"
     CDS             complement(8593..14226)
                     /locus_tag="PLJ75_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03773.1"
                     /translation="MKSLSNIIIAGAILTSITAIVISQQLSSTFHERINNYIDTHKEQ
                     TVQPSARGLDDVLILQPQQELSPFEYNSGDWTKYIIEPRYSFSIISNQIYEEEPYDIL
                     SYGSMNINVNYGKSYFTNDKYKRFDEDKPTSALIQSGLTIEQNSQLHIEGKAGKRLTL
                     YIDHDSQQQNNTYVLQYKAVNDDEVIREINAGDINIKVNNSKYAVYDDTTSKGVGIDF
                     TIKKGDLKLKAFGSVSKGVSEIERFKGNYSNTSVLLQEYQYTKGTYYQLEPFKRFDNA
                     DAPPANPYSTITWNSNPPSPATYSPYSVGIDSSGFELYADEASSENKTGLMAIYKWND
                     TSIVLGYFKKLQQGSDFTINFATGTITLLKNLKSQAKLFAVYTRNGTTQTSDPSAITS
                     HPQFSGKICVFLKYASSLHEDLNYNGVLDSGEDSNNDGMLNYDIYEIRGMYATGLQQL
                     RSDDTKLSFFNNLFPMSKTDVERLGGIIIDYNTGIIQFKLREPFKQLLSIQSQKVIYS
                     ETQSSTAYTVSQFKIGISGYSQSRGFQLKHINIIPDTVRVKINGVQLAESLYSVDPTS
                     GFFQFTNPGNPVITQDTDIEISYQYLPFEGQSQEFMGGVRADYALNKNISVGGTILYT
                     TQGSASTIPVIGNEPTQTTVVEADSTIDLTQHDISSFATKMSNSNVTIPVSFRGYVEY
                     AKSYHSINTFGKGLIDDFESIFSGLTLSMSEKDWILGSLPDSISQNMRARIYYKFYRD
                     INNPETLYGLHFTPINIPYSKKPGPYNVATGHIADSIQSENSQRSLALNFDFSSGDYI
                     SIVTQKLAVVPADLSNLEYCEIWYRSTGGTGTVNLYLDIGTINEDSDGDGIFDTEDTN
                     RNGYLDYDPNKSTSEDVGYIFNNTFPTVVGSGPGLSSITIGDGVLNTEDLNNNGMLDT
                     AEQYIRIPGLLTPISIDCSNTTWQKIRIYINKSNPSQYTSSAEAFEDILKKIYSIRLL
                     LNHDTASTGTILIDSVRFVTMHWQITEIDGTTPGDTNKASITLIDSYNDSEYAAESFA
                     LQKNNVYTSLYGDKTKKEIQKTIESALKVSYNSINSTTLIRKFYKPMDIRFYKYIHCW
                     SNFRNYTSGDSIHFKLLSSDNDYIEYAYTPQFVHAWEDIVLSLQGEITNAQFIKKEGS
                     PDLKRIIAISVTVHNTNTSGEFWLDDIYASDPITIEDTAHWYEGTIKITKPLITTQEG
                     TPVLSDIMIEYVKKGHGNDFYTIGQPYNDISEDYSQAVFSCNILPYWHTSIDYLQEQT
                     QTDSLNENVIPTRRGTTSINQLHFISTLIPPDKRIPQIDMMYNYEQFDNQQEYKQSAN
                     SYDISSNKITHQAIIGLQQTLNNILGGDLSYRLLLDTSFKDDTYKENSESIFNGFSNQ
                     KKQRQSCNVSIQYQFPHLYISPGIQVLSEEFVTYRGTVSDINPVIYHEINSNFHPPFY
                     YDDTIRFTERLKKSSLTIGLQRYQFINPSVSYEFSYFENQFKDFLPSEIWYDYGFNRS
                     HSTYGFIMNTLSLPINFSKIFPSIKGCMFTYTRSGTINEINVPYEGESTNFYNERYGV
                     KRFTSATANEICNVISYPPWYFFKGRSNYANSRDYVTYTMNTQPAIGGVHFADYNNYF
                     RLLDNAAFSINWELSPLILFLNSSVNSVSDRNGINTPAQQVVTYSIVSSVSCDLMKIF
                     SFGFFRPNKPDLPYHSAILLLEYQFNKYLRITSNIVQDEHTPTIGTTFKWDRSSVAVK
                     FGISYRTQKWYEFIPIDDRKRDNDDTIYFENMTVQPDFTNIDEGYTFSTIYETDVQFI
                     YDFFKHWYTLTAMPIFRIEYYMALNRYNYTFYTSPEPYDLYSISSNLTVNVHKNIQGS
                     IIARGILERFRNRETNSINREIVSYELGFQFSLLF"
     gene            complement(14269..15261)
                     /locus_tag="PLJ75_00720"
     CDS             complement(14269..15261)
                     /locus_tag="PLJ75_00720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD03774.1"
                     /translation="MKVRIKIFKSNIERKLKEKYYLKKKQWLKKWDKVLEHGHEKMTI
                     MFIPHNEQKIFNFQISRFTILFFTTLLVIVLVTSSYAIIKNTAVKREEERLLSNYKDI
                     RSQIIRFEQLTNELGQELDKIKPDIEDLYDLATNNNNSTSIWESSVVNNEDPSMRQYA
                     SILPSDIQVLRSMQKDLLCTTNTVKTIKNFIDVRNKVIYDTPSIIPNPGHITSLFGWR
                     RSPFGFDRDFHSGIDIAAPQGTEIRATAPGIVISASWGGGYGYMVRIRHKYGYETIYG
                     HCSKIIVSQGQSIKKGQIIAYVGQTGSATGSHCHYEIRIGGVAINPYPYMSKMW"
     gene            15468..15818
                     /locus_tag="PLJ75_00725"
     CDS             15468..15818
                     /locus_tag="PLJ75_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPD03775.1"
                     /translation="MECKRCGNCCNDVRLAESPEVLQRAYKYWLQLSMINPNFSEIYL
                     IYPMLTFKYQNLSEDLPYHYSCKHFTFDSHGLGLCSIYDIRPRMCKDFPYYDGIALAP
                     EEKLSPYEGCGYNE"
     gene            15827..16933
                     /locus_tag="PLJ75_00730"
     CDS             15827..16933
                     /locus_tag="PLJ75_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD03776.1"
                     /translation="MPQFVEYIAFFAGGISLLVAVGQLCVPHRRLENLNLFGLFLVLG
                     LLLFQYGCILNGFIFKKPYLFYFHFTFLYCIGPLLYFAYYLVIRIEDSLPKINLLLLL
                     PSVIYCIIDIFFIRSDYNLQIAIINSLFFDNFGIYHWLRIAVIAALLLDSLYIGFLLH
                     RLFQFWKLHELTSIMVVTLIFSISSFAGLMILLASYCMRSLIGIKVSVIIMAILLIAA
                     YLVGQHKPEFLQLLIREARRKRYERTLLKGCEVESLCEKLKQLMDEERLYLDEDVTLK
                     EVANELNITAHQLSELLNVKLQMNFNTFINQYRIKEAKKMLIEESERSVLSIAYAVGF
                     NSKSSFYEAFSRFTGKTPVQYKREKKNCTYAINE"
     gene            17069..17611
                     /locus_tag="PLJ75_00735"
     CDS             17069..17611
                     /locus_tag="PLJ75_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPD03777.1"
                     /translation="MSKEFQYKLWAIAFYSFVAAYSYLVLTAGSGNDILFWSYEEFIF
                     GISISIILALLTFWLIPIKVTAALLNPLLYIGLILYFVGPFFLSLLIANVEVMYRIIT
                     GKIQPAIVKVDPEMDDELGIYLLANSITLSPGTLTAGYDPDKKKFFIHCLYWVKDKGY
                     KATPKDVSGFQHYFLKKLFK"
     gene            17625..17894
                     /locus_tag="PLJ75_00740"
     CDS             17625..17894
                     /locus_tag="PLJ75_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPD03778.1"
                     /translation="MYDFDLIFLISGLLLIIYVCIIVIRIAFSHNVPERIVALDTINT
                     LIIVIMIVLGAAQKKALYIDIGIVYGIISFIGTLYIARYLIDEKR"
     gene            17897..18304
                     /gene="mnhG"
                     /locus_tag="PLJ75_00745"
     CDS             17897..18304
                     /gene="mnhG"
                     /locus_tag="PLJ75_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012572546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPD03779.1"
                     /translation="MDTIICIFLVIGLLVNGVSIIGLMRFPDIYTRLHAATKTTTFGS
                     IFVVLAVGVKNIWYYDATSITIIIHSIVALLVIIFTNPISAHAIARASLLSGIKPFGV
                     VINEYDYKSQIKTVDDLEMDKISEGIPDEEGTL"
     gene            18301..18543
                     /locus_tag="PLJ75_00750"
     CDS             18301..18543
                     /locus_tag="PLJ75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPD03780.1"
                     /translation="MIYYFFLVISIALVITALLSIYFRDLLAAIVSYSVFSLVLTVLY
                     FLLDAPDVAIAEAGIGAALTTCIFVIAIRMTRRREE"
     gene            18540..19367
                     /locus_tag="PLJ75_00755"
     CDS             18540..19367
                     /locus_tag="PLJ75_00755"
                     /inference="COORDINATES: protein motif:HMM:NF006248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPD03781.1"
                     /translation="MRLISVVAIVVIFGFIFVGILHLHPIGEPIGNPRILYERQPLGI
                     DTQGVTIMDDYFLRNGQVETGSNNIVASIVFDYRGFDTLGEATVLFLVVSSISMLFFT
                     FLKSKSIISAGVSKAGQFPKIESRIITFGTFLMYIMILSFGIYLVVHGHISPGGGFQG
                     GSVMASATALLLISFYITRHMQRTRKIFSLFESLGLTIFIGMGFAGIGISFLYNFLAH
                     GTTVFGKIIPFGPNPGYLISAGILPWLSLAVGIEVFFGLSLILVTLFHASGVKNNSL"
     gene            19377..19733
                     /locus_tag="PLJ75_00760"
     CDS             19377..19733
                     /locus_tag="PLJ75_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015283649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HPD03782.1"
                     /translation="MSAIILYITFVLLFTIGLFALIFKRNLIKIVIALNILDSGMNLF
                     FITLGYRHNATSPIFTLLPWDTHITMVLPTPQALILTNIVIGFATTALMLGVAILTYR
                     NNKTLDTRKLRGVEEL"
     gene            19730..21256
                     /locus_tag="PLJ75_00765"
     CDS             19730..21256
                     /locus_tag="PLJ75_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPD03783.1"
                     /translation="MIKHVPVLLVTIPLVVAFMLPIIGMVNRTIRTITAGVAIVVIMV
                     LSYLLIQDVMIYKKIIHYMVGSPDPNALLEHGLTFPIRIALKIDQFSLLFIIMVTTLS
                     FIFYLFSSQYIEENERKNYFTVLYVCMVAGMMGMLITNDLFTFFVFLEILSISSSALV
                     SFRTERKHPPYAGYKYMFVSSIATSFFLLGIAFLYAQYGSFNIDYLKKVMNGTVLDKI
                     AIVMLILPLAMKCGVVPMHMWIPDTYSEAPAPISSMVKLGSLICLYGLFRIAFTFGLS
                     RAQVSYELGMLFILFGVLSMFVGVTQALIQKDVKRLMAFHAVSQVGYMLLGVGVGLSV
                     IKSGDASFAIYGKIAIIGGLFHVVNNALYKGLLFLTSGIIVRHYKTRDLNIMHGLAHY
                     DKFTALVFMIAALAISGIPPLNGFVSKLIIYESVFHYNPLLTLIAIFVSVLTLASFMK
                     VFYSAFLGMPPERRPVEQPILVKCSMIILSIVIIVIGLFPQGIIKLLIEPAVNVLSTF
                     "
     gene            21269..21607
                     /locus_tag="PLJ75_00770"
     CDS             21269..21607
                     /locus_tag="PLJ75_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03784.1"
                     /translation="MHEFELEYLNWMPLLWVCIFLIIMVLFIIIGKIFFRNDFDEMSE
                     QGLPFYSGTSDKMLQLITASNLYWGFKHTLQSYFDGLNRLFISDIGDFIQWFIIIVAI
                     MLLIITGALM"
     gene            21655..22029
                     /gene="nuoB"
                     /locus_tag="PLJ75_00775"
     CDS             21655..22029
                     /gene="nuoB"
                     /locus_tag="PLJ75_00775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008082271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPD03785.1"
                     /translation="MNTGSCNACDIEILAMLMPRYDAERFGIKLVGSPRHADILLVTG
                     TVTEKIKDKILQVYQQVPKPKLVMIIGSCGATKGVFQESYAMAGPFDTLLPVDVYVPG
                     CPPRPDAIIFGVAKAWEKLEVL"
     gene            22053..22547
                     /locus_tag="PLJ75_00780"
     CDS             22053..22547
                     /locus_tag="PLJ75_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPD03786.1"
                     /translation="MDVQSIVTIIQEKLGNHLQASRLKVKNYHGGIMRLHLWISIDRE
                     AFHFTVNTLKSLGRLHLSTAMAYKEYEDRIELIYPFALKMDDTHYSELMIIASVDLPN
                     NDLTIRTITDIVPGALFMEREVMEMLGIVIEDIPDSRRLFTPSSLQDGYFPLRNGKNK
                     EDSK"
     gene            22544..23773
                     /locus_tag="PLJ75_00785"
     CDS             22544..23773
                     /locus_tag="PLJ75_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPD03787.1"
                     /translation="MKSDRVYPLNIGPIHPAFKEPIKFTFRIEGEQVTGVDIDFGYTH
                     RGIEKIALERNYIQIIYLLERVCGICSFSHPLAYCLAIEDVAEIQVPDRARYIRTIIG
                     EMERLHSHLLWTGVAAHEIGFDTLFMYTWNIREKVLDCLEELTGNRINYGMLTIGGVR
                     RDVTPENSETITRMLEYYMELYDRASEILLDDLTLRARTEGIGILSREQAEMFCAVGP
                     TARGSGLLKDVRIDYPVNAYYDIPWLNPISPRDLGKEPIGDVYDRMAVRVLEIKQSLN
                     IIQFCMENLPEGDIHSGDGAVKIINTLKKLEGEGIGRYEAPRGEVCHYVILDNQEHPI
                     QLKVKAPTYSNGFTWIPMLTNIEIADIPIVIASIDPCVACADRMTFIRKGGDEERYNW
                     EYLHQKSIKKYKRSVNQ"
     gene            23770..24783
                     /locus_tag="PLJ75_00790"
     CDS             23770..24783
                     /locus_tag="PLJ75_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPD03788.1"
                     /translation="MIMVLAIALIITIVGLILGLLLKGIDRKIAAYFQSRIGPPLLQP
                     VYDIIKLVYKQTIIPVTAVRWLFSSAPVIALASSVLLLLYISMPYIFYITGIDTTLQG
                     DLIVVLYCILIPSIAIVTGAFSSGSPYAAIGAQREMVILMSIELPIAVVIISVAWHVA
                     DSMADPFAMSSLFEYPLWHNAGAFGVLGGILLFFAMELVLPAEMSRVPFDQAEAETEL
                     AEGVSVEYSGKLLAFILLTDAFKALALSMLVIILFFPYTLSQLFGISFQMYGHRVAPF
                     VEIAFLLFKIIVIYSFGITFIRVIMARLKISQVARFFLFAITAISIIGLILIMLDPNM
                     RII"
     gene            24794..25258
                     /locus_tag="PLJ75_00795"
     CDS             24794..25258
                     /locus_tag="PLJ75_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD03789.1"
                     /translation="MLGMVYKVIEQIFSKKSTNCFPTKYIPNNTKQYLNQGIIHPPIV
                     VKEGFRGRLEYDFQSCTGCGLCSKVCPANAIELYSAEVNNKKTKRIVIYLTRCTFCKE
                     CVDICPRNSITMTKAFCMADYKKYGNSLVVGSEYRQQCEIKDHTDVVQKKTN"
     gene            complement(25328..26053)
                     /locus_tag="PLJ75_00800"
     CDS             complement(25328..26053)
                     /locus_tag="PLJ75_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD03790.1"
                     /translation="MHIKMKEKQITKYVKTVGKAINTYTMIEAGDTICIALSGGKDSL
                     VCMDALYRRLSYLPIHYTLYCCHVNVTNFNNKPINILQSYCSIYNIPLIIKDITLDLS
                     IKGSSPCFVCSWYRRKALFETAAQLNCKKIAFGHNQDDIITTFFLNMLYHGKLSTMPP
                     KLKMFNGALEIIRPLAFLTEAQIKGYIKALPLSPIENECPYKDCTKRYQMKELLKNLN
                     KTIKGAQTNIFHSLSHWDDDYLL"
     gene            26160..26525
                     /locus_tag="PLJ75_00805"
     CDS             26160..26525
                     /locus_tag="PLJ75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD03791.1"
                     /translation="MARCMIVDDSSFMRRIIHQTLEEAGHQVVAEVDNGLDALSKYKE
                     VKPDVVTMDITMWGKDGLEAISEITSDYPDAKIIIISALNERTLKINEKDINARLFLS
                     KPFEKQELLDAIEKVLNNG"
     gene            26518..27561
                     /locus_tag="PLJ75_00810"
     CDS             26518..27561
                     /locus_tag="PLJ75_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD03792.1"
                     /translation="MDEQLLQLYKKNAIADNAYMIADGTVFLFVTEADKYVLQGKNVI
                     IGATEPVLNSKTYENIHRLETALAVKGTTIKKIPANTIIQSLNNTAFTMNLAIVEAKV
                     VVLTNDILKKNMQIDSQLVKERNICIEYYTIVHSLQLEYEKRKLPWLKDLIDKFKTSI
                     TFKKGEAFSRANESLRIDTPRELSANMIEFPPGSLLCEEGSVGDAMYILESGQLEVYI
                     QNSKVATISEKSTVIGEIAMLLSMHRTATLKAQNTVVVTKITREDLRRSTSPELTQAL
                     LVSLAKKHHANVLHIMDINEKKALAYADSPRQELIKTEAYRYQNELSQLKYTLEKLIQ
                     SKQADYLEYILNK"
     gene            complement(27565..28101)
                     /locus_tag="PLJ75_00815"
     CDS             complement(27565..28101)
                     /locus_tag="PLJ75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HPD03793.1"
                     /translation="MKIEITILGLLMEENLYGYEIKKRIVERLEDYVDIKFGSIYYAI
                     KKALDNGWVKKIGTEKEGGNPERYIYQILPDGRKYYKKMLKQYFDHNFIHFDIDIVLM
                     FLNSLSPEQKEQFIEDRVEHIKDKMNALKEKIDTELKNNSPNSQLHVYTYLENHLKAE
                     LNWLKSLKKGEVLTEVEQ"
     gene            complement(28601..29185)
                     /gene="ruvA"
                     /locus_tag="PLJ75_00820"
     CDS             complement(28601..29185)
                     /gene="ruvA"
                     /locus_tag="PLJ75_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPD03794.1"
                     /translation="MIAFLKGKKISIKPTKVIVDVNGVGYEVTIPLSTYEKISSVATI
                     ELYIAMVVRENSVALYGFSSEEEKQMFAILTSIPGVGPSIAISILSSMSPHYITNAVK
                     AENILAFTAIPGIGKSKSEKIIFELKRKLRKLEATVQHPATTSPDRQDAIDALVSLGF
                     DEKLAMSTISSIITSNPDCDLETIIRLSLKQLSQ"
     gene            complement(29210..29698)
                     /gene="ruvC"
                     /locus_tag="PLJ75_00825"
     CDS             complement(29210..29698)
                     /gene="ruvC"
                     /locus_tag="PLJ75_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPD03795.1"
                     /translation="MATIMGIDPGYGRCGWAIVTDRLTYIDCGVIETTPGDEGERLFT
                     IYQSVGDLIALYQPQSCGLEKLFFSKNTKTALSVVQVIGVCKLILTQHAIPYSEYTPM
                     QIKQSITGYGRSSKEQLSTMLRKILKCDTIHNYDDAFDAIAIALCHCLATPERSLLHQ
                     KV"
     gene            complement(29704..30450)
                     /locus_tag="PLJ75_00830"
     CDS             complement(29704..30450)
                     /locus_tag="PLJ75_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPD03796.1"
                     /translation="MSGHSKWANIKHRKAAQDAKKGKIFTKFIREITVAARVGGDDLN
                     SNPRLRTAVLKARENNMPMENIERAIKKGTGALDGSTYEEVRYEGYGPGGVAIMVDCM
                     TDNKNRTTPEIRTIFSKNGGNLGESGCVSFLFIRKGVINIDANATNEDKVMEILLDYD
                     VEDIKTEDTTIVVYTSPDKFNDILEIIRSHNLPTLNSEITYIPKTTINLEGQKASQCL
                     RLIEALEDHDDVQDVYANFDIPDDIINSMS"
     gene            complement(30576..31853)
                     /locus_tag="PLJ75_00835"
     CDS             complement(30576..31853)
                     /locus_tag="PLJ75_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03797.1"
                     /translation="MNTKLPILITLLLSFFMNSYLFGQNYKDAIHQKKFSEALTIIQS
                     KLDDIYSKRSIEKQIPTTYVAIEKIEEGVDLQKIFSERKIQPYFIENNDTLYALHADA
                     ALCYQNIYKYHEALQHYFQALRFTTIGEKDHTIFYSIAQIFKKINKFNAYVLYLEEAY
                     EIKPDNYDYSLELSLALSSGKNKKKALFHLNRYIASKGDSVPPNLYLIAANCYESIGD
                     YIKASEQYTLYLHHKPNDYAVLFALGYLAYTKISDMKLAYKSFINAIPLFNENDWVLK
                     GLSYTMLGDIECMDLNYKNGLEYYLQSIAISENMKKIIDEKKANINTINDKINVIKST
                     LLANKDIALYPQYQTLMDELGNNELELRQLEHEYSKLNAGLLSFKIAVAHENMMQFAQ
                     AIEWYNKTIEYGIKIRESSKKIEKLQLKISRGF"
     gene            complement(31921..32535)
                     /gene="pth"
                     /locus_tag="PLJ75_00840"
     CDS             complement(31921..32535)
                     /gene="pth"
                     /locus_tag="PLJ75_00840"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD03798.1"
                     /translation="MKLIVGFGNPGEEYFNNRQNIGFKVVDILGNNENIDIRVKKKKS
                     IIGRGKISGEDVVLLKPQTFVTLIGESVLYIASFLRINVRDIICVLEDSSLPLGEIRV
                     DSIMSKLKHPGIESMTKALKSDRFAKVRIGIGYPHKGITMEEHLLNDFSDDENFILID
                     VLNKAEEVVRMLISHSIEEIQNKYNPEGALKIKKRQLPRIRIRR"
     gene            complement(32561..33184)
                     /locus_tag="PLJ75_00845"
     CDS             complement(32561..33184)
                     /locus_tag="PLJ75_00845"
                     /inference="COORDINATES: protein motif:HMM:NF004139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPD03799.1"
                     /translation="MEAQVLPIKTRSEIGKNANHRLRAQGYIPAVLYSHGVSQAVMVE
                     KKNFFKIFKGHISENLLIDLQFNDHSSAPVKAFIKDYQRHPITDEILHLDFFKVTMTE
                     TITTKAPIEIIGTSIGVKQGGILEVIEREIEVECLPADLPEKVQIDVTNLAIGQSIHV
                     KDIIAPNGVKILSSPESVIVAVLAPHKATEEVAQPAGEQPQETVEEQ"
     gene            complement(33260..34207)
                     /locus_tag="PLJ75_00850"
     CDS             complement(33260..34207)
                     /locus_tag="PLJ75_00850"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPD03800.1"
                     /translation="MIYPIKLFSGTSNYALAQDIAHYLGIELSEIEIKKFKDGEISVK
                     ILENVRSVDVFLIQSTSNPANDNIMELLLMVDAAMRASAQRITAVIPYYGYARQDRKV
                     EPRVPISAKLIANMLHAAGVNRVLTMELHSDQIQGFFDIPVDNLFASPVVIEYVNELN
                     IQNPTVVSPDAGGTERARFLARNISAELAIVDKRRPEPNKSEVMNIIGEESVKNHNCI
                     IIDDMIDTAGSIAQAANALKKSGAKDIYCTATHAVLSDNAIEKLKKAEFKEIILTNTI
                     DIPEHKILPNMKILSMAPLFGEAIRRIHNGESVSSLFIR"
     gene            complement(34215..34964)
                     /locus_tag="PLJ75_00855"
     CDS             complement(34215..34964)
                     /locus_tag="PLJ75_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPD03801.1"
                     /translation="MNNCKAVILAAGKGVRMQSSLPKVLHRLAGKPLVQHVIDNLISA
                     GISYNDIIVVVGYKGDDVISAIDNNISFVWQIEQLGTGHAVMQTEPQLGTYNGCLLVA
                     CGDVPLIKPETFIKLYNAVNDHSTGAAVLTMELENPCGYGRIIRGEYDNLIRIVEEKD
                     ADPIQRNIKEVNTGTYAFKSPILFQGLKTITTDNAQREYYLPDVLTFIRKSGYDVKII
                     KLDDPIEGTGVNSKEELIKLGSIITKTFIKK"
     gene            complement(34989..35060)
                     /locus_tag="PLJ75_00860"
     tRNA            complement(34989..35060)
                     /locus_tag="PLJ75_00860"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35026..35028),aa:Gln,seq:ttg)
     gene            complement(35098..35982)
                     /gene="ispE"
                     /locus_tag="PLJ75_00865"
     CDS             complement(35098..35982)
                     /gene="ispE"
                     /locus_tag="PLJ75_00865"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPD03802.1"
                     /translation="MIESIKAYAKINLHLEVLNKRPDSYHNICSIMAKINIFDLLQLE
                     GCELCNPNTIDITIRCIGGQNAAIVKELPIEQNLITKAIRLYCSTAHIGSRVTIAIQK
                     EIPSGAGLGGGSADAAAALMLCNNVFNKFSHNELMALAAEIGADVPFCLNGNIALCEG
                     KGEIVTPLKKIPLPDAVVIVFNDVHSSTKEAYTALMRSFAPVLSKKEAVLSLWDKSDV
                     GALLNIFKNDFQDIVFDNNPQLREIYRTLQDFAPDFIHMTGSGSALCALFHDIGKANE
                     VFLTLKPHYSQVFVSNLL"
     gene            36513..38630
                     /locus_tag="PLJ75_00870"
     CDS             36513..38630
                     /locus_tag="PLJ75_00870"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD03803.1"
                     /translation="MNIKIYVSALAMKLYLIPLLVACLFFAGCSPTEVFFSSYSAQCG
                     VFDCSTIDFTKEKPVELKGEWKYRMLEDSPAFARPDYDDSEWDYIIIPNYWNSEKFKG
                     DGYGWYRLKVKVNKSFWETSPAIYVPGANTAYELYFNGNKFMCSGIVSIDEQSSSPCL
                     KPQLKPLNCEKETEEFVIAVKVSNFFHRGGGLNKCIVIGPFESITQILWEHDFIKTVI
                     LGIIFIIAVYHLILWMGRRKDKTSFWFSIFCFAIFSHSFVIGNYLERIFYNSDVFFIR
                     FSIEYGSLGLGWLSFTLYVCYLFPRESNRMFVIFQIITGIIFISIPMILPPKIFTNLI
                     GAYNVILFTTMIGAFIILIAASIRRRDDALLIFIGYIVLMITVANDILHNLFIIRTFF
                     MSEYGLALFILFQSSVLSLRFARAFKTTEYLSENLNAEVKKKTEELLNQTEKAISAQQ
                     DTEKTYKKLASLYDQISNEVHLAKTIHDRIIPKEIMHIPDVKIAVYNEPFMQIGGDVY
                     DIASLPNKKTRVFLADATGHGISASLITILIKVEYDKLKDECETPAEVFKKLNTVFSD
                     YYKTMKMFLTAIMVDIDGNNNKLLYASAGHPDQFLVMNSTIFELSRTGPAIGISKFSN
                     FENKIIDIKKNYKLYLFTDGIIEEFSTDYFGNQYKDLKHFILENGHLSVEQTVSLINE
                     AIKRNASNIEHRDDKIFIGIESI"
     gene            38831..41176
                     /locus_tag="PLJ75_00875"
     CDS             38831..41176
                     /locus_tag="PLJ75_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPD03804.1"
                     /translation="MNKKVLLAIKNNNREAITKLITSTPGISGVNYNNKHYEIEYDSS
                     VISPIHIVELLEENGVYIEKEIVQATIGDMSCVMCSKAVTNAIMDIEGVLDINVNYAT
                     GKAMLVVTPEAVHDSDIKTKVEDAGYRFLGTEEVQTQKQSPLRLIQIVAGLVAGALLM
                     AMMYGLWAMDTIGIAIILTPVFLFVSYHIFTGAYHALKNRVLSMDVMYAMGIGVSFGA
                     SILASLHVLSHEFMIYETSIFLASFLNIGKYLEERAKSRTSHTIAKLLSLSPDTAIVI
                     RDGNEIEINTSDVTIGDTVVVKPQTRIPVDGEIVKGQSYVDESMVTGESVPVFKSEGN
                     AVIGGTLNKNSLIVIKAKAIGQDSILSRIINLVEHAVMSKPRVQRFADRVVSIFIPVI
                     LTIATIAALVWYVINGDIFFSFEVFVAVIVIACPCALGLATPTAITVGIGKGAELGIL
                     IKNSEALENASKVTHAVFDKTGTLTTGTMTVTNIIAFDNISEHELLSVCASLERYAEH
                     PVGEAIVQYATNHHAVFHEVEDVSVVEGKGVMGTIAQKKVIAGSRDFIQDYIPCEVTI
                     AQKMKEFENEGKTVVVVAQEKPYGIIALSDTPRTDANTVIRSLQQRGIKTAMLTGDNR
                     LSALAIAHEIGMDAVYADVKPEDKMNVVKQLQKDGEIVAFIGDGINDAPVLAQADVGI
                     ALGSGTDVAIEAGDVVLLRNRLRDVVTYFELAVKVMRRIKQNIFWAFAYNAVLVPVAA
                     GVLYPFTGWLVKPELAGAAMALSSVTVVSLSLMLKRFKPGI"
     gene            complement(41160..41735)
                     /locus_tag="PLJ75_00880"
     CDS             complement(41160..41735)
                     /locus_tag="PLJ75_00880"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPD03805.1"
                     /translation="MNTINTTIITLAQQIQEQFPHFYLAGGTAIMLKYNHRISYDLDF
                     FNFKEFSYNRLAKKVIPLYQNQIDKWERKEDNIDFFINGIKVSFVFFPFKNLEKIETI
                     HHIKVAGDFDLFLNKLYVAGRRIDPKDPFDAAYLYNQHNWDNNYIIQGFEKKFKGQSY
                     KIYLGALLSFEDYGELPAWIKETLMKLIYQV"
     gene            complement(41722..42003)
                     /locus_tag="PLJ75_00885"
     CDS             complement(41722..42003)
                     /locus_tag="PLJ75_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03806.1"
                     /translation="MQQQNSGIQKPQRIKPFYSQKVPIPSNLTSLLWDEQDTAPLEKL
                     ILRVLQYGNYEEIHTIYSLYSTETTDIVSRYPDIKRGVKFWVRYWNEHH"
     gene            complement(42179..42535)
                     /locus_tag="PLJ75_00890"
     CDS             complement(42179..42535)
                     /locus_tag="PLJ75_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPD03807.1"
                     /translation="MLIITTIIGLSAGMLSGLMGIGGGIIMIPALIYVMHFNQHLAQG
                     TTLAAMIPPIGILAAYAYYKSGYVNIPVALTLALGFIIGGYFGGKIAVSLDSETLRRI
                     FGIILFVISLHIIFSK"
     gene            42624..43325
                     /locus_tag="PLJ75_00895"
     CDS             42624..43325
                     /locus_tag="PLJ75_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPD03808.1"
                     /translation="MAKNTCIVTCEHAGNYIPPELTALFSGEHEIVATHLAYDIGALP
                     MAMIIASILHVPLIYTKVTRLIVDCNRSVGSDELFSKFTKNFDSVLKNELIRRYYDKH
                     RNTIEQAVSRAMRHHTHVIHVAVHSFTPQLNGFKRYCDIGLLYDQYCPVEQQLSKSIK
                     ETLFALHPLRIAYNYPFRGCGDGVTVWLRKQYGDRYCGIEVEVNQDLYFQGNHLWRQL
                     SSNIGQAIKRAMTTV"
     gene            43444..44841
                     /locus_tag="PLJ75_00900"
     CDS             43444..44841
                     /locus_tag="PLJ75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPD03809.1"
                     /translation="MVVDMTMAQKKLKNIKAEYAEIRMSESDTVSIHLSKNRVETCKA
                     GKTTQGSVRVYNRGCWGFVSFTTLDMIDYAIIRAQELSHIQKKDDAVSIAAMQSNVVN
                     KKTKVLKPLHSVSLEEKMELMHRYNAILKSSPLVENTNVVYRDSMTHYYMYNTDETGV
                     DYESSFCGVSFSAIAKDGTVMQPYGESIAQYGGFELVEDNHAVAEEVVKTAAELVKAE
                     QVQSGLYNVIVDPKLAGVFIHEAFGHLSEADFIYENPRMKELMVIGRRFGPECLQVID
                     DGSLPYTGYIPIDDEGIQPQKTYLIKDGILTGRLHSRETAKKMGEQPTGNARAISCGA
                     HPIVRMTNTYIDNGTATADELFDRLGNGLYVAGALGGQTNLEMFTFTPAYAYEVKRGK
                     KTRLLRDCMLSGNVFMTLMNIDSIADDCTMFGGLGGCGKQGQAPLPVSSGGPHLLIKN
                     VLIGGSGAGGSKKKS"
     gene            44813..46135
                     /locus_tag="PLJ75_00905"
     CDS             44813..46135
                     /locus_tag="PLJ75_00905"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPD03810.1"
                     /translation="MLEAAKRNHDAIRKECQGVQCRWWDIYSNDTISKSVTFKNNVFH
                     SARESAVRGYGIRLNIHGKTGFSYCNDFNDIKTVIAHAKDTAQYGDNEDYALPGKNAI
                     PKVTLYDEQWLESWTVENQVQQVQAIIDTIRKAYSDCMVDSSAGCVDGYVYLTNSSGF
                     EAGYRYLQHGVSVSGLRIVSDGSRMQVYESTSWGSTFDYHEIVNRLIMKLQFSETNAA
                     IPSGNYYAILTPKAFGQIMGVVLQGLSGKSIQRGISRYIGKFGQKVFGKQLSVYDNPL
                     EHFAPGSYPFDGEGVMAFNKTIIECGYVQRHIAHLQSSHILQCNPTGNASRSYATLPQ
                     ESFSNIVVPGGDVSFKDMIRQMDTGIIIDQFIGFGQSNTLMGQFSANLDMAWFVNNGE
                     VQGRLKNCMVADDVTRMLDDTIVLSKEREWIGDSYLPYVMCRIDYTTI"
     gene            46215..47300
                     /locus_tag="PLJ75_00910"
     CDS             46215..47300
                     /locus_tag="PLJ75_00910"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017221940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPD03811.1"
                     /translation="MEKRHTYSNLYIKEYSMKTRITELFKIQYPIVLSGMSWISTPEL
                     VAAVSNAGGLGILATGVLTADQTREAVQKIRSLTKKPFGANVTLYFPGAERNAQVLIE
                     EKVPIINYSLGKGDKIAKAAHAYGGKVVATVTTHKHAFAAQRDGADALIVTGYEAAGH
                     GGAITSLVLIPAIADALDIPIIAAGGFADGRGLLAALALGADGIAMGTRFMNTKESPA
                     AEQAKQASVKSEVYDTIYTPKVDGLPARFMKSKGVEHLLKKRLNPFSALIRSKKIADM
                     LEFPWLKLAIGIMLMGPKRSMQMAQMAIGFDAFKLGTIDGDFAKGVLPLGQVSGIIKD
                     TPTVKQVVDSIVKEAVAIEKQLSKKVK"
     gene            47312..49198
                     /locus_tag="PLJ75_00915"
     CDS             47312..49198
                     /locus_tag="PLJ75_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD03812.1"
                     /translation="MKSTTELSSKNIIAKGFVMQKATLQGLHDFLSEEKTVVSMLLSR
                     ADARGDSIALTGFKGDEKYSYTWSELKHHAYAIGYFLISHGCQSGDKIAIFAQNCPEW
                     TIVDLGIQSSGCVPVPIYATNSKDEARYIIEDAGIKVVFTGDQEQFDKILEIATMSSV
                     THVISLQDDVTVTSEKGFYVKDIINTTVDSAVIEQVTSRMRGLSSNDMLTIIYTSGTT
                     GNPKGAVHTHASFLAGIYASVVRFPEAGPGMVSLAFLPLSHVFERMWSYGVLLKGGEN
                     HYCRNPKDIMAILPLSKPHYMCSVPRLWEKMYATIFDRLESASPVKKKLFVWATNAGI
                     EFDTRKRSNKFINPFLLCKKAIGEIVVLKKVRDVVGGRASVFHVGGAAMSADINEFFN
                     GLGIPLAQGYGLTEFFPVAVGTDKTAYPGICGPVLDIVDVRVSDEGEIQLKGPMCMDG
                     YYNKPEATREMFTSDGWFKTGDVGTVEKHNDNLYIRITDRIKDLIITAGGKNIAPQVI
                     ETMLGEDLYIEQVVVVGDGRKYISALIVPNFEMLHEYAKSKGIQYSSRQELISHPGIL
                     EFYNNKIEKLTEGLGQVEKVKRFTLMPAEFTQDNGEITPTMKIRRKVIQQRYSDVINK
                     MYED"
     gene            49316..50353
                     /locus_tag="PLJ75_00920"
     CDS             49316..50353
                     /locus_tag="PLJ75_00920"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005495120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPD03813.1"
                     /translation="MMKTRITQLFGIKYPIILPGMSWISTPELVAAVCNAGGTGYLAT
                     GPLTAEQTRAAIQRIRELTNKPFGAGCTLIMPGARENAEVMLEEKVPIINVSLGKCDW
                     IAERAHKYGGKVIATVVTEKHALAAEKQGADALQVTGHEAAAHGGQVTTFVLVPAIVD
                     KVKIPVVAVGGIADGRGMAAALALGAEGIGMGTRLSMTKESPLHKKTIEAQLKAGIED
                     TIYSNRFDGIYCRVLKTKSAEKAIKKGMSLPHAAIQSYKIAKMMNLPYFKLVLGVMLQ
                     GPKMMIQLAHFATAFDKIKVATEDGNLEYGVQLIGQCQGLINDVPAVKDVIERTVKEA
                     KAIYQKNLKKF"
     gene            50434..50589
                     /locus_tag="PLJ75_00925"
     CDS             50434..50589
                     /locus_tag="PLJ75_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03814.1"
                     /translation="MTINKQVQDKIMKATVIVNPSSRSGLKVYQTLTHYMDNYYYHED
                     QIFILIP"
     gene            50757..51203
                     /gene="rplM"
                     /locus_tag="PLJ75_00930"
     CDS             50757..51203
                     /gene="rplM"
                     /locus_tag="PLJ75_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPD03815.1"
                     /translation="MSIWQRTFSLKKSQIEKKWYIIDAEGKVLGRLAAEIAHVLRGKH
                     KPTYSAHLDMGDNIVVINAEKILLTGKKPDDKEYFFHSGYPGGMKFVNIKKMLQKKPE
                     YVLYHAVKGMMPKNKLSRNIMGNLKIYAGKDHPHQAQKPENLELKF"
     gene            51246..51638
                     /gene="rpsI"
                     /locus_tag="PLJ75_00935"
     CDS             51246..51638
                     /gene="rpsI"
                     /locus_tag="PLJ75_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPD03816.1"
                     /translation="MANAVNYATGRRKSAVARVWLKPGNGAIVINSKPINEYFRRQTL
                     ELVVRQPLEEAGVLASFDVYATVNGGGWTGQAGAVKLGIARCLSALDEKYKKSMRQAG
                     FLTRDSREVERKKYGQKKARKRFQFSKR"
     gene            51725..52366
                     /locus_tag="PLJ75_00940"
     CDS             51725..52366
                     /locus_tag="PLJ75_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPD03817.1"
                     /translation="MVIIDIEYSDRYIMITLDSGEHLKIDSVSVPQGLLRKGTEIDID
                     AYEHIKEESKLFECKEKALYYIGLRDRSEHEITKYLIKKGFDKNTIYRVIKLFIEKGF
                     IDDYHYALQYVRSTHEHKVVGNRVIEKKLFERGIHKSLIKKAIKKMQDNEPDIERIYQ
                     IAQKKYNRVKGKEHAREKVGLFLQQRGFSWDTIKEVLNRLGDNNSDNNNFIDD"
     gene            52638..55274
                     /gene="alaS"
                     /locus_tag="PLJ75_00945"
     CDS             52638..55274
                     /gene="alaS"
                     /locus_tag="PLJ75_00945"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPD03818.1"
                     /translation="MMKVHDLRVAFIEYFKQRDHRIVPSSSLIPQDDPTLLFTTAGMV
                     QFKPMFAGTINAEYTRAASIQKCLRTSDLANVGKTKRHCTFFEMLGNFSFGDYFKKEA
                     IQWAWEFSTQVIQFPEENIWVSIYTDDDEAFDIWHTIIGIPEQKIVRLGKEDNFWGPA
                     GDSGACGPCSELYLDRGASFGCGKPDCKPGCDCERFLEFWNLVFNQYFQDTEGKLHPL
                     PKTGIDTGMGLERLATLVQNVDSIYQTDELNELVNYVINKRNIRYEGDAQVAVNVLVE
                     HARTLTFAISDGAYPSNEGRGYVLRRILRRALRYSRQIGIHEPFVYTMVEPIVTIMGK
                     QYPELSSTKENVKAVIKGEEERFLETIENGMDRLDEIVNALKKKGEKTIQGNDVFVLY
                     DTFGFPVEMTEEIAQEHGMTIDKVGFEKKMEEQRTRGRQSWKGAEGSIDTIVGQLAKI
                     IQRTEFEGYEKYDSESDVVAMSNFEDLKVQLADGERGIIVVKNTPFYGESGGQVGDTG
                     YIYGDNGTFRVEDTKKSGDLIIHVGQMEKGYLQEGEHVRLSIDGIRRNCIRANHTATH
                     LLQAALRNVLGQHVKQAGSLVEPERFRFDFTHFKAMSHDELLQVETMVNEKIWACLPV
                     ETSVMPLQDAIHAGAMAVFGEKYSDIVRMVSVNNFSKELCGGTHVDNTGKIGVFRIMH
                     EASPGAGVRRIEGVTLKGAYERLLHDQHLLGRVMRLLEVNESNESNVEKKITEMVEKV
                     SLLEKELEKTKEKGLKSIAKDLYDKAETINGIKFVSAVLQNMDAEALRSVADFVRELS
                     VAVVVCLASSHDEKAFIIAAANKIAVDKGIDCNAIIKAVAKKIGGGGGGRKDLAQAGG
                     KSPENVGIALSMCKEILLNSFK"
     gene            55367..57277
                     /locus_tag="PLJ75_00950"
     CDS             55367..57277
                     /locus_tag="PLJ75_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD03819.1"
                     /translation="MGELKTMVRYFYDTCDRLPDRPAQKFNKELYDDNNGLFTYAELK
                     ERIEDIACALMKLGFPYKGRAAILAPTSYMWTQVDIAIACAGGASITIFPTLSNNEVK
                     YIMNDSRCTIVFVGDKTLLKQVKELEKDLKSLKTIIVMDMSFKGDKKKVFGIKDCIAM
                     GREWRNNQANYKKYNKRKDSVTLDDWFTILYTSGTTGQGKGVILTHWCGSSRLEGTTE
                     YFQRYNFDVNENDVTLCYLPLSHVFDRGSCQWLAISKGSLICYADKPATLIEDMQKYN
                     PTWINCVPRLYEKIYITMQQQMAENPLQKKLFNWALNVGLQALKYREDENGCYNMHPN
                     FDLKSRLPLWLKIQYSIADKIFAKVRGLFGKRFHHSFSASAAISPDLLRFYYALGLAV
                     IEGYGSTESFNAAILNPITACKPGYMGKEANGSKCRVSKDGELELSGAGIFKGYLNKP
                     KETKETFTKDGWFKTGDIVELSSDGYFKFIERKKGIICTSIGKNVAPAKIENLFSTSN
                     WVEQIFLVGDEKPFITALIVPNFANFMALFKREGITYDESKLVYEDVGGMQLCVQVGD
                     DFINNYRLKELIDNDVKEANKHLEKFEQIKNYTILNQRFTESNGMLTPTQKTKKRVIV
                     EVFKDEINKMYA"
     gene            complement(57347..58705)
                     /gene="gdhA"
                     /locus_tag="PLJ75_00955"
     CDS             complement(57347..58705)
                     /gene="gdhA"
                     /locus_tag="PLJ75_00955"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPD03820.1"
                     /translation="MSVISEVIDKVKRQDSEQKEFIQAVHEVLETLEPTVQKHPEFVK
                     AKIYERIVEPDRVIIFRVPWVDDNGNVHVNRGFRVQFNNAIGPYKGGLRFHPSVNLGI
                     IKFLGFEQIFKNSLTTLPMGGAKGGSDFDPKGKSDMDVMRFCQSFMRELYRHIGPDTD
                     VPAGDIGVGAREIGFMFGYYKKIRNDHTGVLTGKGLEYGGSLLRPEATGYGATYFAAE
                     MLKTVGKEIKGMTVAVSGSGNVAQYTIEKVTQLGGKVISCSDSNGTIIDEEGITPDKL
                     AFLMELKNVRRGRVKEYAEKYKNAHYFEGKSVWDVIKEQGIKISVAFPSATENEIDGK
                     HAEALIKNGCICVSEGANMPSNLDAIKVYREHNILFAPGKAANAGGVATSGLEMSQNS
                     LRLSWTREEVDNRLQIIMKSIHKSCLDASEAYGKKGDYLTGANIAGFLKVAKAMVAYG
                     IV"
     gene            complement(58945..61533)
                     /locus_tag="PLJ75_00960"
     CDS             complement(58945..61533)
                     /locus_tag="PLJ75_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD03821.1"
                     /translation="MDTINKASTGLPCLDKIVNNLIMGDNVVWQVDTIADYKTFVDPF
                     VSKALSEGRNLNYIRFAEFPPLLFEQPGIKIHYLNAHEGFESFSKRIYTIINREGENA
                     YYIFDSLSELQSAWATDLMIGNFFVITCPYLYELNAVAYFSLLRNSHSFKTIARIRDT
                     TQLLIDVYTCNGDCYIHPLKVQNRYSPTMFFPHKKNADYFTAITRSDDVTTFISHIQQ
                     TSLEKTQRNLDFWDRLFLKAEMLLELNEDPTKKQQMIEQLCKVIIGREERLLTLAANI
                     FTLEDLIAIKNRMIGTGHIGGKAAGMLIARRILHSDNRMQWNDIVEPHDSFYIGSDVF
                     YTYIVQNGWWKLFMEQKTQEGYFTVAKEIAQQMLHGIFTDDIKEQFQQMIEYFGQSPI
                     IVRSSSLLEDSFGNAFAGKYESIFLVNQGSPAQRYQAFIDAVRQIYSSTMNEDALVYR
                     LQRGLHTMNEQMAPLVQRVSGTHRGNYFFPDISGVGFSHNTYVWKKEMKPESGMIRLV
                     IGLGTRAVNRVEGDYPRIVALDDPRSKPLYDFKDVRRFSQHNVDVLNITTNQVETIQM
                     DDLFNIQDLNLPMHLLGIRDYEAEQTIKNMNGEQKEYWVVTFDELLMHTDFTSNIKTI
                     LQTIHNRYQYPVDIEFTVNFNKEEGYYINILQCRPNQIKGLGKKVKLPEDIPGDHIFF
                     TSRGNFLGGSIVQPIKRIIYVDPYLYNSLSISQKYDVARTVGKLNKAIQDREEMPALL
                     IGPGRWGTTTPSLGVPVRFAEINNIKVLVELALINENIMPELSFGTHFFQDLVETDIF
                     YVALFYGISNVLYHQNFLEQFPDIFTKLLPDSQYNHVIQVFDLKEHDVELMADITTQK
                     VLCFMS"
     gene            complement(61669..61935)
                     /locus_tag="PLJ75_00965"
     CDS             complement(61669..61935)
                     /locus_tag="PLJ75_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03822.1"
                     /translation="MLLPDPQTCINKINDFNHGITQQRTARDNCDYITLFQLIVNNIS
                     NNNSIPISCNLIRMLGLFQAYRNQPAQKNLNFISNLLNELGSSY"
     gene            complement(62160..63578)
                     /locus_tag="PLJ75_00970"
     CDS             complement(62160..63578)
                     /locus_tag="PLJ75_00970"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPD03823.1"
                     /translation="MKHVNFFLLIILVVVNLTGCSNDLFLIDIGKYGYIDKSGKIVIN
                     PQFEEAYDFSKEGLALVKLSDKYGYIDKSGKIVINPQFEKAYSFGEEGLAVVMLNKKY
                     GYIDKSGKIVINPQFEKAYDFSKEGLAAVKINQKWGYIDKSGKIVINPQFEDAYSFGK
                     EGLAAVKINQKWGYIDKSGKIVINPQFEDAYSFKEGLALVKLSDKYGYIDKSGKIVIN
                     PQFEDAYSFGEEGLAIVKLSDKYGYIDKSGKIVINPQFEDAYSFGEEGLAIVKLSDKY
                     GYIDKSGKIVINPQFEDAYDFGEEGLALVKLSDKYGYIDKSGKIVIDTQFYYAEEFKN
                     GLALVGGEKYGYINKSGKYVWSYDKGADIYLGKDIVGWHITKKTDIFNSDDYFIMILD
                     NHYKFIGVTKIELRISVVKDAGVEQPFRIISYPIDSRAPMWGFNKKSITAGAFVGNRK
                     GKFELLIVGDNKTLASKIFEVR"
     gene            complement(63792..64640)
                     /locus_tag="PLJ75_00975"
     CDS             complement(63792..64640)
                     /locus_tag="PLJ75_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03824.1"
                     /translation="MKKILLPLIITVFIHTNIYSYDLLKIGDNVTNYIKTNPVKYKLT
                     NQKLSNWPEGVVTLLAYDTKVDGKSPIIVFALDKYPIGVLQGTSPNAYYIFDIDGDGI
                     LDTKTDKGIVPFWVVYANSKNKSKNDNITSIFNLVYESFQSDEGPYINQKFKESINKL
                     YEFNKNINIENRDLAYLFLFYQLYNQYFPEQSLKCLDLLTKEYISRFNKIHPILLLYK
                     AETLINLKQEQDAKKIIAELRKADPNFIPAKAYEYRLEVDKEKAKILLNNLKENHKNH
                     WIVKQL"
     gene            65344..65478
                     /locus_tag="PLJ75_00980"
     CDS             65344..65478
                     /locus_tag="PLJ75_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03825.1"
                     /translation="MNDLEIREAWIIAPVKETYMLTKTVRVAALYEFVSALAEGSSKS
                     "
     gene            65941..66492
                     /locus_tag="PLJ75_00985"
     CDS             65941..66492
                     /locus_tag="PLJ75_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03826.1"
                     /translation="MRSVKLIDVRQEESKTLNEFGRQKIINFLISKMGKNKFSQMVES
                     AKKNNQFDNEQWFSFVLSNLGSSIAFVLELNMCERCYDKSILYNAYYEVPVPDQSEEK
                     EAGTNGYSDATGILDIKGISNEDGNNPSNRNIVPVDIIKSFSKEEDVIKLKKGEVSPL
                     QGVLSRIEYEYEYGIRKITIRLK"
     gene            66525..66773
                     /locus_tag="PLJ75_00990"
     CDS             66525..66773
                     /locus_tag="PLJ75_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03827.1"
                     /translation="MATAGFIVGILSLFGTMLGCIPLLGALNWVNIPFGFLGIILSGI
                     AMASSNKQTKGKATAGLVMSIIAVIFGAIRLMMGGGIV"
BASE COUNT        21064 a        10839 c        13366 g        21844 t
ORIGIN      
        1 ttttcataaa aaacgcttat atttacatat cgaatacttg tgataaaacg attagcattt
       61 gtgagggttc aatgcatgaa ttatctatca tgtccaatgt gcttgacatt gtggtgcagc
      121 atgcacagca gaatggggcg gtgaaggtta caaaaattca tttattaatt ggtgagctat
      181 ctgattatat ccctgaatgg atgcagaact attttgattt tgtaagcaag gatacaatag
      241 ccgagaatgc ccagctggtt gtggagatgg tgaaggcatc gttacaatgt ggtgattgtg
      301 gcactgcatt tgcatttaat aaagatgact ggcaatttag ttgtccgaaa tgtcattcgc
      361 caaatgtaac gattgtttca ggcagggagt taacggttaa gactatcgag attgagggag
      421 catgaagcat gataactgtt gtcatggttg tttgcgataa tagatcatat agacaggaat
      481 gtaaagacct ttcaggaaaa gcaattgagc aataggaacc atgcaatact tttaaacata
      541 aaacatgcaa tgacgtcatg gataattgca gaccctcaat acgtcaacaa tcattaattt
      601 gtcaggtaat gctaaagctg ttaatgagaa tctatcgatt atatggatat gctaccgaac
      661 accatacaat tgttgcaagg acaggaaagg attgcttcta ttgcttccat gaataaaatt
      721 tgattgcatt cacactttat tcataccgat actattacta tatcgtagtc gttcaacgct
      781 gtggttaaca ataaagagta caataggaat tgatggaatt gtagcctatg ttaaaaataa
      841 cattcatcat aaagcatggt atggctgttt tgctttgctt atgtatattc cagtgtattg
      901 gatatgcagg ccctattgta aagattgaac atgttgatat aacatcattc cccattgtta
      961 gtatttccgg taagctccat gcaatccaga gaagaaatag tataacaaaa gataatttta
     1021 ccgtattgga gaatgggaag gttataaagg aattgtcact gaagcatgga cagctggata
     1081 atgtatatat agccttatgc attgatacga gtaaaagcct ttcaaaaaat aattttttgc
     1141 gtttaaaaaa tagtgccata agcataataa agaaaagtcc ggattactat aaatttctac
     1201 tgtataaatt taataatgag gttgtgcaat tagcgtcgca tgatgacgat agtatggtgc
     1261 atgttgcaaa aataaaaaat ttgcaacagc ttggaaagtg tacccagttg ttcaattgtt
     1321 tatatactgc tattgacgat ctttctaagt ttgatggagt caaggcaata ctgcttttta
     1381 ctgatggtaa ggatgaagga agtgatatgc ttcccgacga tgttatcagt attgcgcggg
     1441 aacactcaac ccctgtttat actgtactgc cgcaaggttg caggaaaaca gctgttgtga
     1501 gtcgtttgag caaagcagcc aacggtaaat tgtttataat aagtgacgat atcaataaaa
     1561 aaatactcca gcttatgcaa tccaggatat catcgggcat aatccctttt gagctgagtt
     1621 ataactcatc aagtaccgat ggtattggct ctgtggaagt cagatttaac aatggtactg
     1681 tcagtgataa cgatatacaa tatataaact ttaaagcacc ctcgtttaca gcatataggt
     1741 tcaataatgt gccttttgta gtactggcag gaattattgt gctgcttata gttgcactgg
     1801 taattgtttt tattattttc atgaataaat ggaaacacat tattgaactt gtcagtatgc
     1861 agaggatacc acggccagta caacattact atgatgtact ttttgaaaaa gatgaagctg
     1921 gaagaaagga agaatcaaac ttattgctgt caagctcacc tgaatatagc tataccagtg
     1981 catggctggt agagaaaagt ggtcctgaaa caggaaaaaa atttccctta atctgggatg
     2041 aggtaaccat tgggcatggt aaggaaaata cgatagttat caacgatgat gctgtgtcgc
     2101 tgaaacatgc aaagataaaa aagattaatg actgctatta tctctttgat tgtgccagtg
     2161 ataatggcac ctttttaaac aacaagaagc tcctgcgtcc aaaaatgctc accgattggg
     2221 acgaaattca aataggaaga accacaatac tttttagagg aacaaagata aaacactaat
     2281 gccatgccat taaaagtatt actatgctca ccgccggtct ttgattttta ttatagcttc
     2341 catcgttctg agccgctggg ccttttatat atcaaagcaa tgctacaaca gtatacctgg
     2401 ctggatgttg atatctatga tgcacggcag aaagcaaaat caaagaagct tcaaactcct
     2461 gatgtttttc agtatctgaa ccatatctat atgcacgata cctcatggtt tagcctgttt
     2521 tgtggatata agcgttttgg gtcttcgttt gacaggatag ttggtttgat taatgaaaaa
     2581 aagtatgatg tagtatgcat atcttcttta tttacagcat atcaccacga tgttgagttg
     2641 ctggttaaaa agatcaagga gcatagcagt gcgatagtta ttgtgggagg ctgggctgta
     2701 tggagtatgt ccaccgttga aaagggatat gctgatgttt atgtaaaaga tgatggcgag
     2761 cttgtattgc cggagctgtt gcatactatc cacacctcaa ataataagtg tatgagtaaa
     2821 cttcccaaaa ttatcaaatc cagcaatggc aggagcaatg aattttttag taataacttc
     2881 cctgagcgat gtggatggta tacatactat ggtaaaagga ttgccaatgt catctgtagc
     2941 aagggatgca tctatcaatg tgcattttgt tcaatacatt cgcgatatac ctatcaacgc
     3001 agagcattcc agtccattga agaagaattt cactatctct cgactaacgg tgtgcagata
     3061 attaattttg aggatgataa ttttttatat accagggatt atgctctaca gttactatcg
     3121 ctcatgaaac gttacaaaga aaaagggatg aactatcttt gcatgaatgg cataaccgct
     3181 tcaaacctcc atgcagtact tgatgatgcc atggagtccg gatttttgga gtttaaccta
     3241 tcgcttgttt cagctaatga tgttattgcc aggcgctata accgtccgct gataaaagaa
     3301 atgattcagg atatagcctt tcgatgtgca ggcagggtga aggttattgt ttatgttata
     3361 gcaggtttgc ctggcagcag tattgatgat tgcatacagg atattgtatt gctggcaaag
     3421 ctcccggtaa taattggatt ttcaccactg tatatgattc cgaggatacc tatgtttgaa
     3481 actatggggc ttccggagga tatgaggttg tgcagaggga gcgcgctcta tccgtttggc
     3541 aatgatatca cacgagagga tattgcaagt ttatggaagc tgtgccgttt cattaataaa
     3601 atgaaatatc tcccacacaa ccctgatgat atacatttgt taccggagca tatctactac
     3661 tacaccagaa catgcaggga gaaaagatgg tattacaaaa gcagtgatgg cacctggcag
     3721 cagggttttt cattccaagc ctcgttacca gaacatatcc atgtttgtga tattaatggg
     3781 aatgctcgtg aaatgtgatg ctgtagtttt ttgctgtttt actctttata cataataaaa
     3841 caatagttaa atccccgaag aaaaaaattg ccttcttcag cagccctgtg ataattgccc
     3901 attgtcagtg ttttattatg tcgtgctaca gcgatgatat caacaggttt gaagtattgc
     3961 aacagtatgg agaatagttt gaatcctata ggaataaagc catccttctt gttataataa
     4021 tcacaaatat ataatcccat ggagtgtttt ggtttacaaa tgcgatgtat ttccttaata
     4081 actatcgcca tggcatcaaa gtactcctga cttttagcgc tcagtttccc aatacactgt
     4141 tcttcgttgg agtagctgat attatcgcca tacggcgggt ctataaagat aaaatcagcg
     4201 ctgttacttt tacagggaag attgcgtgca tcagctttca taatatcggc tctatacgga
     4261 tgaatgtcat accccagaca tttccgatgg agatcgttgc atacatcaat ggttgtcccg
     4321 ctgccgcaca ttgggtcaac aaccatatca cccttgtttg tataccgcat gagcaaattc
     4381 catatgacat atgaaggggt tgcacccggg taattttgat caccctgctt ttgattgccg
     4441 taattctggg aggggtagtt ccataatgtt gtggtctgta atgttagtgg cggtttaaac
     4501 atgatagtaa cctcaagagc tattatttac aatactatat caaaaatttt tctcattttc
     4561 agataaatcg caaaataaga agattttaat agtaaactat gctattacaa acgtaaaaat
     4621 gtaatatttc aatactttgg gggatgacat gaaaaaagat acacatgaat gtgttagcaa
     4681 atgctatgtg ctctatggta atctggtgaa aaaaataata gccggttact ttacatccaa
     4741 tgaggatatt gaagatatct tccatgatgt atttcttaaa ttagtgaggg ttgatttaaa
     4801 aggggatccg tttgatcctg ctataaagca ttatattgtt aaagcaacac ggaatacctg
     4861 catcagccgt attcgtaaag aaatgcgaca ccataacaat ggcgacagtt actggctatc
     4921 tcaaattgca actattgaag aggttaataa taaagatatg gctgaaacaa tgattgatgg
     4981 tatggtagtc aatactctgt atgatgttat aaaggaattg agcaatgacg agcaatcatt
     5041 gctgtacaaa aaattttacc agaataagaa attttcagaa attgccgcag agtgtaaatc
     5101 aacatattat tttattgata aacaattgaa aacaatttat aaaaagcttt ccctgaagat
     5161 ggttcggaca tgtatagacc ataatgatat atcataaatt ataaattagc ttgaaattta
     5221 tcgataatta ttgtaaaagt acttcaattt tatatcatgg gattatatat aatatattct
     5281 ggatggttta gctttgaact acactataat tactatctgt gcaattactg ttatatttgc
     5341 tctctttgtt gccatcatta atatagtgaa aagatattct tacgcagtaa accgcttgtt
     5401 ttcgcttatt gccattctga cagcgggtat ggtttcatgt ttaattatac catattttat
     5461 tcctgatatt gacattgttc aactgacgcg ctggtatttt ggtttaataa taattattaa
     5521 ccagggcttt tttcattata cacaaatttt ccctcgatgg gaaaagaaaa gccctttatg
     5581 gatgattata acaactgcta taccaggctt tattacattt gtactttgcg tggcaactga
     5641 tacaataatt acccatgcat catttattga gtattatttc aactttagtt atggggtatt
     5701 gattgaagta tatctagttg tttttatttt ttatatcatg ggtacttttt ttacattatt
     5761 ttataaagtg aggcgattag aaaatacttc atttaggtat caattgttct atatttttat
     5821 gggaaaccat tttggtgcat tgatcatttt gataacgttt ataattttac cgtatgcttt
     5881 taaaatatat gattttcaca gccctgggat tgcatccgct gccttgatat tgttactgat
     5941 aaataattat gcaatttcag atgaacgcta tcttgatgtt aaggaattct actttaaaat
     6001 agttttttat tgtatagtat tggctatgac ggtaattcca tcatatttta ttcttgtata
     6061 tagttatagg ttttttttga gaggtgggca gattcctgcc gtagctgttg cttttatcaa
     6121 tgttatatat ttatttgtat ttacacgttt tatccagcca tatctggaga aatcctttaa
     6181 acgagagtat gtacgtctgg aaacacgttt taatacattt attcaggcat tatctcaggc
     6241 agaagaattt gaaggggatc cagcatattg ggataaattt ttttccacaa ccattgatag
     6301 tctggaaaat ttgtttaatg ttagttctgc atcacttttg atgtataatg agtcagaaaa
     6361 agcatttatt ttaagccaca gcattggtga acaaacaaaa attaaaagaa tagtgtctac
     6421 cgattctatt gtgcagttat gtgagatggt cacaacaaca atccatcaat cattatttta
     6481 tacggatgac actatcaaag cttttgatga tgtccttata ttggtaaaga gtaataatat
     6541 tgaagttatt cttcctattt ttgacgcaaa tggaaaactt gtagcgatat tattggtggg
     6601 tcctcataaa aaagggcgcc catataccta taatgagatt caattcatgg aattatacag
     6661 agtgaagttt ggtaatgctt tagtcaattc tttgcagatt gaagatgtga aaacaaagca
     6721 ggttgctgaa catgatcaat tagtggttaa tgctattaaa acttcaatgc aacccaaaca
     6781 aataccgttt atcgatcata tcaggataag ttctatcttg atgaataatt caaatctggg
     6841 tggtgaattt tgcaatgcgc gagttttacg caaaaatttg ctgggatttt taatagctga
     6901 ttgttcatat agtggagtag atgctgctgt attagctctg gagttatatg gggcttttga
     6961 atcgtgttca cctgtttatg atgctcccga gaaaataatg cagttattaa actgggttgt
     7021 gtgtacctcc agatttactg atagatatgc tactgcattc tgtttcacat ttaattcaga
     7081 tgatctatcg ttaaaatatc tcaatgcggc atttaatcct ctcattctct atgagattcg
     7141 ttctgacagc tttttagaat tggataccaa agggataccc atcgggattg aacgcgattt
     7201 tatttatgaa tccaaagaat atcgagcaaa tggaccatgt ataggctgta tctattccaa
     7261 tgggattgcc ggtgctgtta atgctgacgg acagacatat agtattggca ggataaaaga
     7321 tttaatccgt ctgcataata gtgaaacccc ggctttgatt atacgacgta tttcatctga
     7381 tctcaatgat tttataagta atacagcatt aaaagaagat atcagcattg tgatttttcg
     7441 tattgagtga atgcatatcg ttattttcga aaattttata tgaacattga ttttaaagag
     7501 tcagctcata aaataatatc tatccttcaa gtgcaaaaac atattgcgat tttcattaaa
     7561 ggatcgcctg accctgatgc aatagcgtct tcgtactttt tgtattgttt atgtgatgca
     7621 ttgcatgtca aagcaacgat gtttgctttt atgcctgcat cgttaccatc caatagagaa
     7681 attatatctc tattttctat tccattatat attgaagatt ccataaaggc tattaacctg
     7741 caacgctatt caggatatat tgtatgtgat tttcagacag ctttaattga tggaataata
     7801 aatgctattc cctgccttat ccatttagat caccatgcac cggtggagga tgatgtacct
     7861 gctcattttc gtattttaac tgatgaagta tcatcgacaa gcaccttatg tgctctcatt
     7921 atgcaatata tatcatgctt ttcacaggat atgatacact tactggcaac ggtattatat
     7981 ttgggaataa agacagattc tgataatttt aatcatttaa acaaatatga tgtaatggcg
     8041 atagaatatt taaagcagtt gtatatacct tcaatgattg aaaaaattga gagtgtcccg
     8101 ctgtcactgt attctcaacg gttactacaa catgcaatgg ctaatgtggt gatatataaa
     8161 gactggctaa tagcaggtgt tggatttgtt gaagaggaac atcgagacac tattgccata
     8221 attgccgatt accttttgga aaacaatcat gttgaacttg cagttgttta tggagctgtc
     8281 tatcataata atcatacaaa attagatatt gatgcatcgc tcagaacaaa aaatgaagat
     8341 cttgatttaa atgaattgat caaaaatata acaaccgatg gaggaggaag aaaatataaa
     8401 ggagcattcc aggtgcatat agattatttt gtgtattgcc ctgatcagga aaggttgtgg
     8461 gatattattg cattgaccac tcatcatatt ctaaaaaata tcagagatgg aatttataaa
     8521 attaccatcc agaacaaatt taaaaaatta aaaaagacat tgaaaaaatt attcattaaa
     8581 gaaaagtaga ttttaaaata acaggctaaa ttggaatcct aattcgtaag aaacaatttc
     8641 acggtttatt gagtttgttt cccggtttct gaaacgttcc agtataccac gagctataat
     8701 tgatccctga atgttcttgt ggacatttac agtgagattg gatgaaatac tataaagatc
     8761 atagggttcc ggcgaagtgt aaaaggtata gttataccga ttaagggcca tgtagtattc
     8821 aatgcgaaaa atgggcattg cagtaagcgt ataccaatgt ttaaaaaaat cataaataaa
     8881 ttgtacatcg gtttcataga tagttgagaa tgtataacct tcgtcaatat tggtaaagtc
     8941 aggctgaact gtcatgttct caaagtaaat ggtatcatca ttatctcgct tcctatcatc
     9001 aatcgggata aattcgtacc acttttgtgt gcggtagctt atgccaaatt taacagcaac
     9061 acttgatctg tcccatttaa aagttgtccc tatggttggg gtatgttcat cctgtacaat
     9121 atttgaggta atacgcagat atttattaaa ctgatattcc agtagcagta ttgcagaatg
     9181 gtatggtaaa tcaggtttat ttggtctgaa gaatccaaaa gaaaaaatct tcatgagatc
     9241 acatgaaaca gatgaaacaa tgctgtaggt tacaacctgt tgagcagggg tattgatacc
     9301 atttctgtct gaaactgaat tgacacttga gtttaaaaaa agtataaggg gcgacagttc
     9361 ccagttaatg gaaaatgctg cattatcaag gagtctgaag taattattgt aatctgcaaa
     9421 atgcactccc ccgatagcag gttgagtgtt catggtatag gttacgtagt cgcggctatt
     9481 tgcataatta gacctccctt taaaaaagta ccatggaggg tagctgataa cattacatat
     9541 ctcatttgcg gttgcactgg taaaccgttt aacaccatat cgctcattat aaaaattggt
     9601 agattcacct tcatagggaa cgtttatttc attaatagtt ccggaacgcg tgtaggtaaa
     9661 catgcaacct ttaattgatg ggaagatttt actaaagttt ataggtaatg acagggtgtt
     9721 cataataaat ccataggtgg aatgcgagcg attgaatcca taatcatacc atatttctga
     9781 tggcaaaaaa tctttaaatt gattctcaaa atatgaaaat tcataagaaa cggatgggtt
     9841 aatgaattga taacgctgga gcccaatggt aagtgaactt ttttttaatc gctctgtaaa
     9901 acgaatagta tcatcatagt aaaaaggcgg atgaaaattt gagttaatct cgtgatatat
     9961 tacaggattg atatctgaaa cagtgcctct ataggtaaca aattcttctg atagtacctg
    10021 gataccggga gaaatataca aatggggaaa ttgatactgt atggatacat tgcatgattg
    10081 gcgttgtttt ttttgattgc taaagccgtt gaatatagat tcactgtttt ccttataagt
    10141 atcatcctta aacgatgtat caagcaacaa cctatatgat agatcacccc ccaatatatt
    10201 gttcagggtt tgttgcaatc caatgatagc ctgatgtgtt attttattac tactgatatc
    10261 atatgaattg gctgattgtt tatattcttg ctggttatca aattgttcgt aattatacat
    10321 catgtcaatc tgtggaatcc ttttatcagg aggaatcagg gttgagatga aatgtagctg
    10381 gtttattgat gttgtacctc gtcttgttgg tataacattt tcattcaggc tatcagtttg
    10441 tgtttgctcc tgcaggtaat ctatagaggt gtgccagtag ggaagtatgt tacacgaaaa
    10501 aacagcttgg ctataatctt ccgaaatgtc attatatggc tgtccaatgg tatagaaatc
    10561 attgccatgt ccttttttta catattcaat catgatatca gaaagtaccg gggtcccttc
    10621 ctgggttgta attaaaggct ttgttatctt tattgttcct tcataccagt gcgctgtatc
    10681 ttctatggtg atagggtcac ttgcgtagat atcatcaagc cagaattcac cactggtatt
    10741 ggtattatgt actgttacac tgatagcaat aatccttttt aaatcagggg atccttcctt
    10801 ttttatgaat tgtgcattgg ttatttcacc ctggagcgat aaaacaatat cttcccatgc
    10861 atgaacaaat tgcggggtat aagcatactc tatataatca ttatccgagg aaagtaactt
    10921 gaagtgaata ctatcaccac tggtataatt tctaaagtta ctccagcagt gaatatattt
    10981 ataaaatcga atatccattg gtttgtaaaa ttttcggatt aaagtggttg agtttatgct
    11041 attgtatgaa acttttaagg cggattctat tgttttttgt atctctttct ttgttttatc
    11101 gccatacaga ctggtgtata cattgttttt ttggagtgca aatgattctg cggcatattc
    11161 gctgtcattg tatgaatcaa tcagggtaat ggaagctttg ttagtatcgc cgggagttgt
    11221 gccatcgatc tcagtaatct gccagtgcat ggtgacaaag cggacggaat caataaggat
    11281 ggtcccggta ctggcagtat catggttcaa gagtaaacgt attgagtata tcttttttaa
    11341 aatatcctca aatgcttcag cagatgaggt atattgagaa gggttacttt tgttaatata
    11401 aatccttatc ttctgccatg ttgtgttaga gcagtcaatt gagattggag tcaataaacc
    11461 agggatacgt atatactgct ctgcagtatc aagcatacca ttgttgttga gatcttcggt
    11521 attcaacacg ccgtccccta tagttattga cgatagtcct ggccctgagc caaccactgt
    11581 tgggaaagta ttattaaata tatagccaac atcttcagag gtgcttttgt ttggatcata
    11641 gtctaaatag ccatttctat tggtatcctc agtgtcaaaa ataccatcgc cgtcagaatc
    11701 ttcgtttatt gttcctatgt ccagatatag gttaacggtt cctgtgccgc cagtacttct
    11761 gtaccatatc tcacaatatt ccagattgga aagatccgct ggtaccacag ctaatttttg
    11821 tgttacaatt gaaatatagt cacctgaact gaaatcgaag tttaatgcca gtgaacgctg
    11881 ggaattttcg gattgtatgg aatctgcaat gtgaccggtt gccacattat atggtcctgg
    11941 ttttttagag tagggaatgt ttattggtgt aaagtgcaat ccatacagtg tttcggggtt
    12001 gttgatatca cggtaaaact tataatagat acgcgcacgc atgttttgtg aaatactgtc
    12061 gggcagtgaa cctaagatcc aatccttttc tgacatggat agtgtaagcc cggaaaaaat
    12121 tgattcaaaa tcatctatca aacctttgcc aaatgtattg atactgtggt aactttttgc
    12181 atattcaacg tatcctctaa atgaaactgg aatagttaca ttactattgc tcatttttgt
    12241 agcaaagctt gagatgtcgt gttgcgttaa atcaatagtg ctatcggctt caacaacagt
    12301 agtttgcgtg ggttcatttc ctataacggg aatggttgaa gcacttccct gcgtagtata
    12361 aagaattgtt ccgccaacac taatattctt gtttaaagca tagtcagctc ggacacctcc
    12421 cataaattct tgagactgtc cttcaaatgg gaggtattgg tagcttatct caatgtctgt
    12481 gtcttgtgta ataacaggat tgcctggatt ggtaaattgg aaaaatccgg atgttggatc
    12541 cactgaatag agactttcag ctaattgtac accattaatc ttgacacgga cagtatcggg
    12601 gataatattg atatgtttta gctgaaaacc ccgtgattga ctgtatccag agatgccaat
    12661 tttgaattgt gataccgtat aagcagtaga actttgtgtt tctgaataaa ttaccttttg
    12721 tgactgtatt gataatagtt gcttaaaagg ttcacgaagc ttaaattgta taattcctgt
    12781 attgtaatca ataataatcc cacccaacct ttcaacatct gtctttgaca tgggaaaaag
    12841 attattaaag aaggataatt ttgtatcgtc gctcctcagt tgctgtaatc cagtagcata
    12901 catgcctctg atttcataga tgtcatagtt tagcatccca tcgttgttac tatcctcacc
    12961 actatcaagt actccgttat agttgaggtc ttcatggagt gatgaagcat attttaaaaa
    13021 tacacaaatt tttccggaga attgtggatg gcttgtaatg gctgaagggt ctgatgtctg
    13081 ggttgttccg tttctggtgt acactgcaaa aagttttgct tgtgatttta gattcttgag
    13141 gagggtgatt gtaccggtgg caaaattaat ggtgaaatca ctaccctgct gtagcttttt
    13201 aaaatatccc aatactattg atgtatcgtt ccatttgtaa attgccatca atccggtttt
    13261 attttcagat gatgcctcat cagcataaag ctcaaaaccg gaactatcta tcccaacact
    13321 atacggagaa taggttgccg gtgacggagg atttgaattc catgttatag tagaataagg
    13381 attggctgga ggggcatctg cattatcaaa tcgcttgaag ggttctaatt gatagtatgt
    13441 tcccttggtg tattgatatt cctgcaataa gacacttgta ttggagtaat tgcccttaaa
    13501 tcgttcaatc tcacttaccc ctttggaaac agaaccaaat gccttgagtt taaggtcacc
    13561 tttttttatg gtaaaatcaa tgccgacacc ttttgaagtt gtgtcatcat atactgcgta
    13621 tttagagttg ttaaccttaa tattgatatc gccagcatta atttcacgta ttacttcatc
    13681 atcattcact gccttgtatt gtaaaacata ggtattattt tgctgttgac tgtcgtggtc
    13741 aatatacaat gtaaggcgct tcccagcttt accctctata tggagctggg aattttgctc
    13801 tatagttaaa ccagattgaa taagtgctga tgttggtttg tcttcatcaa agcgtttata
    13861 cttatcattg gtaaaataag atttgccata gttaacatta atattcattg aaccatatga
    13921 aagaatatca tatggctctt cttcatatat ttgattgctt ataatggaaa acgagtatcg
    13981 aggttcaatg atatatttag tccaatcccc tgagttatat tcaaagggtg atagttcttg
    14041 ttgaggttgt aaaatcaaaa catcgtcaag ccccctggca gagggttgta ctgtttgttc
    14101 tttatgagta tcaatgtagt tgttaatgcg ttcatgaaag gtggatgata gttgttggga
    14161 aataacaata gctgttatgg aggtaaggat agcaccagca ataatgatat tggataatga
    14221 tttcatgtgt tagagtaata accatgtttg caacaggaaa atggacaatt accacatctt
    14281 gctcatatac ggataaggat tgatagcaac acctcctatc ctgatttcat aatggcagtg
    14341 actgcctgtc gcagaaccag tttgcccaac ataagcaata atctgccctt ttttaataga
    14401 ttgcccttgt gacacgataa tcttggagca atgcccatat attgtttcat aaccatattt
    14461 atgtcgtatt ctgaccatat aaccgtatcc accaccccac gatgcggata tcacaatgcc
    14521 aggggcagta gcgcgtatct cagtgccttg aggagcagct atatcaatgc ccgaatgaaa
    14581 atctctatca aaaccaaaag gggaacgcct ccaaccaaac aacgaggtta tatggcctgg
    14641 attggggatg atagatggtg tatcatagat gaccttatta cgaacatcga taaaattctt
    14701 aattgttttg accgtgttag tagtacagag taaatccttc tgcatgctgc gcaatacctg
    14761 tatatctgaa ggtaaaattg aagcatattg acgcatcgat gggtcttcat tgttcactac
    14821 cgaactttcc catatggaag ttgaattatt gttattggta gccagatcgt aaaggtcttc
    14881 tatatcaggt tttattttat caagttcttg acctaattca ttggttaatt gttcaaaacg
    14941 tattatctgg gaacgaatat ccttgtaatt ggaaagcaat cgctcttctt ctcttttaac
    15001 tgcggtattt ttaattattg cataggagga tgtaacaaga actatgacca gtaaagtagt
    15061 gaaaaaaagt atggtaaaac gcgatatctg aaagttaaaa atcttttgct cgttatgtgg
    15121 gataaacatg atggtcattt tttcatgacc atgttcaagt accttatccc atttttttaa
    15181 ccattgtttt ttctttaaat aatatttttc cttcagcttt cgctcaatat tgcttttaaa
    15241 gatttttatt cgtactttca tcaatactat ttctcactat agttataaat aataattatt
    15301 caatttgtta tgatacatca attcatatga ttttaacaaa agataaatat atataataaa
    15361 ttgcaactat aataaaatat gctattatta tatatatcgt atgatagata taataattat
    15421 gcaagttttt tttggtagta ttttattaaa tcgttgaagt gaattatatg gaatgtaaac
    15481 gatgtggaaa ctgctgcaat gatgtacggc ttgcagagtc acctgaagtt ttacaaaggg
    15541 cgtataaata ctggttgcaa ttatccatga taaaccctaa tttttctgaa atttacctta
    15601 tctatcccat gttaaccttt aaatatcaaa atctttctga agatttgcca tatcattatt
    15661 cctgcaaaca ttttacattt gactctcatg gattggggct atgcagcatt tatgatataa
    15721 ggcccagaat gtgcaaggat ttcccctatt atgatggtat tgcattagca cctgaagaaa
    15781 agttaagtcc ttatgaaggg tgtggatata atgaataggt aaatgtatgc cccaatttgt
    15841 agaatatata gctttttttg caggtggtat ttcgctttta gttgcagttg gtcaattgtg
    15901 tgtacctcat cgcaggcttg aaaacctgaa tctttttggg ttattcctgg ttttaggtct
    15961 tttattgttt caatatggat gtatattgaa tggatttatt tttaaaaaac cgtatctttt
    16021 ttatttccat tttacatttt tatattgtat tggtccactt ctttattttg cctattacct
    16081 tgttatacgg attgaagatt cacttccaaa aataaattta ttactgctgc tcccttctgt
    16141 tatttattgc ataatagata tattctttat acgttctgat tacaatttgc agatagccat
    16201 tattaatagt ttattctttg ataattttgg tatctatcac tggttaagaa ttgcagttat
    16261 tgcagcactt cttctggata gtttatacat cggtttttta ttgcaccgtt tattccaatt
    16321 ctggaaattg catgagttga cttcgataat ggtggtgaca ttaatattca gcataagttc
    16381 gtttgccggg ttgatgattc tattagcaag ttattgtatg cgaagcctta ttgggataaa
    16441 ggtaagtgtc attataatgg ctatcttgct tattgcagcg tatctggttg gacagcataa
    16501 acctgagttt ttacaacttc ttatacgtga agcccgcagg aaaagatatg aacgcacctt
    16561 gttaaaagga tgtgaagtgg aaagcctttg tgaaaaacta aaacaattaa tggacgaaga
    16621 acgcctgtat ttagatgaag atgtaacgct gaaagaagta gccaatgaac ttaatattac
    16681 cgctcaccag ctttcagaat tgctgaatgt aaaactacag atgaatttta acacatttat
    16741 caatcaatat cgtattaaag aagcaaaaaa gatgcttatt gaggaatctg agagatcggt
    16801 gttgtcaata gcatatgctg ttgggtttaa ttccaaatca tctttttatg aggcattttc
    16861 acgatttact ggcaaaaccc cagtgcaata taaaagagaa aaaaagaatt gcacatatgc
    16921 tatcaatgaa taataagaat tatgaagagt cttaatttat aaatcaagct aaaggaaatg
    16981 ataaaaaaag cttgtattaa gaataaatag tgtgtatggt ttacataatg gtaggggtat
    17041 acaaattaaa attaatcagg gtaatattgt gtcgaaagaa tttcaataca aattgtgggc
    17101 aatagcattt tactcatttg tagcagctta ttcatatctt gtacttacag ctggttcggg
    17161 caatgatata cttttctggt catatgaaga atttatattt ggtattagta taagtattat
    17221 tctggcttta ctaacttttt ggttaatacc tattaaagtt acagcagcac ttcttaatcc
    17281 attgctctat attggattaa tcttatattt tgttggcccg ttttttctat cgttactcat
    17341 tgccaatgtt gaagtaatgt acaggattat aaccggaaag attcagccgg ctattgttaa
    17401 agttgatcct gaaatggatg atgaactggg catctatctg cttgctaatt ccataacatt
    17461 atcacctgga acattaacag caggttatga ccctgataaa aagaagttct ttatacattg
    17521 tttgtattgg gtcaaggata aaggttataa agcaacacca aaagatgtat cggggttcca
    17581 gcattatttt ttaaagaaat tatttaaata atatggatca acatatgtat gattttgatt
    17641 taattttttt aatctcaggg cttttattga ttatctatgt ttgtatcata gtaatacgca
    17701 ttgcattcag tcacaatgtg ccagaacgaa ttgtagcact tgacacaata aatacactaa
    17761 taattgtcat catgattgtg ctgggcgctg cacaaaagaa ggcactgtat attgatatcg
    17821 gcattgttta tgggattatt tcatttattg gtacattata tattgcgcga tacctcatag
    17881 atgagaagcg gtaaatatgg atactataat ttgtatattt ttagtaattg gcttgctggt
    17941 taatggcgta agcattattg gacttatgcg cttccctgac atctatacac gattacatgc
    18001 tgccacaaaa acaacgactt ttggcagtat ttttgtagtg ttagctgtag gagttaaaaa
    18061 tatatggtat tatgatgcta caagcataac aataatcatt cacagtattg tagctttact
    18121 tgttattatt tttactaatc caatcagtgc tcatgcaatt gcgcgggctt ctttgttatc
    18181 agggataaaa ccatttggtg ttgttataaa tgaatatgat tataaatctc agataaaaac
    18241 agttgatgat ctggaaatgg ataaaattag tgaagggatt ccagatgagg agggtacttt
    18301 atgatctatt attttttcct ggtgatttcg attgctcttg ttattacagc attgttgagc
    18361 atatattttc gggacttact ggcagcgata gtgagctatt cggtattttc cctggttttg
    18421 acagtactat attttctttt agatgcaccg gatgttgcca ttgcagaagc tggaattggt
    18481 gctgctttaa caacatgcat ttttgttata gcaatacgaa tgacacggag aagagaggaa
    18541 tgaggctgat cagtgttgta gcgatagttg ttatatttgg atttatcttt gtggggatac
    18601 ttcacctgca tccaataggt gaaccgatag gtaatcccag aattttatat gaaaggcaac
    18661 cattgggtat agatactcaa ggtgttacta tcatggatga ttatttttta cgcaatggtc
    18721 aggtcgaaac agggtcaaat aacatagttg ccagtattgt ttttgattat agaggatttg
    18781 atacattagg ggaagccaca gtgctttttc ttgtggtaag tagcatttct atgttatttt
    18841 ttactttttt gaaaagtaaa agtattatat cggcgggagt ttctaaagcg ggacaattcc
    18901 caaagataga atcgagaatt ataacgtttg gtactttctt aatgtatatc atgatcttaa
    18961 gttttgggat ttatctggta gttcatggac atatttctcc aggcggtgga tttcagggag
    19021 gttctgttat ggcttcagct acagcacttt tgttaatcag tttttatata acccgtcaca
    19081 tgcaaagaac cagaaaaata ttttcattgt ttgaatcact ggggctgact atattcattg
    19141 gaatgggttt tgcagggatt gggatttcct ttttgtataa ttttttggct catggtacca
    19201 cagtatttgg taaaataatc ccctttggcc ccaatcctgg ctatttgatt tcagctggta
    19261 ttttgccatg gctatctctt gctgttggca tagaggtttt ttttggatta agccttattc
    19321 tggttacgtt attccatgca tcaggtgtga aaaataattc attataaggt gttattatgt
    19381 ccgctatcat actttatata acttttgttt tattgtttac tattggatta tttgcattaa
    19441 tatttaagcg aaatctcatc aagattgtta ttgcacttaa tattcttgat tcaggaatga
    19501 atcttttttt tattactctg ggataccggc ataatgcaac atctccaatt tttacattac
    19561 tcccatggga tactcacata actatggtct tgcccacccc gcaggcgttg attttaacaa
    19621 acatcgttat tggatttgct actacggcat tgatgctggg cgtagcaata ttaacctata
    19681 gaaataataa aacactggat acgcgtaaac ttcgtggagt tgaagaatta tgataaagca
    19741 tgttccagta ttattggtta cgattcctct tgttgttgct tttatgctcc ctattatagg
    19801 tatggttaat cgtactatac gcactataac agcaggcgtg gctattgttg ttatcatggt
    19861 gctatcatat ttgctgatac aggatgtaat gatttataaa aaaattattc attatatggt
    19921 aggttctcct gatccaaatg ctttattgga acatggatta acattcccta tacgtatagc
    19981 attgaaaata gatcaatttt cgttattatt tattattatg gttacaacat tatcatttat
    20041 tttttattta ttttcatcgc agtatattga agaaaatgaa aggaaaaatt atttcactgt
    20101 tttatatgtc tgtatggttg ctggtatgat gggtatgctt attacgaatg atttgtttac
    20161 attttttgtt tttttagaga tactatcgat atcatcgtct gcattggttt cgttcagaac
    20221 agaacggaaa cacccaccct atgccggtta taaatatatg tttgtgagct caatcgcaac
    20281 gagcttcttt ttactgggta ttgcattcct ttacgcacag tatggctcgt ttaatattga
    20341 ctatctcaaa aaagtaatga atggaaccgt actggacaaa attgctattg tgatgctcat
    20401 tttaccatta gctatgaagt gtggtgttgt ccccatgcat atgtggatac ccgatactta
    20461 ctctgaagct cctgccccaa taagttccat ggtaaaatta ggtagtttga tttgtttata
    20521 tgggcttttc aggattgcat ttacttttgg actaagtcgt gcacaggtaa gttatgaact
    20581 ggggatgcta tttatactat ttggtgtgtt gtcaatgttt gtaggggtaa ctcaggcact
    20641 tatacagaaa gatgttaaac gacttatggc ttttcatgct gtttctcagg ttggctatat
    20701 gttattggga gttggtgtag ggctttcggt tattaaatcg ggtgatgctt catttgctat
    20761 ttatggaaaa atagccataa taggtggact ttttcatgtg gtcaacaatg ccctctataa
    20821 aggattgtta tttttaacat ctggtataat cgttagacac tataaaacgc gcgacttgaa
    20881 tataatgcat ggattggctc attatgataa gtttactgca ctggttttta tgattgcagc
    20941 attagcaatt tcaggaatac caccattgaa tggttttgtt tccaagctta tcatctatga
    21001 atctgtattt cactataatc cgttgttaac attgatagct atttttgttt cagttttaac
    21061 attggcttca tttatgaagg ttttttacag tgcttttctg ggaatgcctc ctgaaaggag
    21121 gccggttgaa caaccaatat tggttaaatg cagtatgatt attttgagta ttgttataat
    21181 agtcattggc ttgtttcccc agggtattat aaaattattg attgagcctg ctgtaaatgt
    21241 attatctact ttttaaatgg agttatgaat gcatgaattt gaattagaat atcttaactg
    21301 gatgccttta ttatgggttt gtattttttt aataattatg gttttgttta taattattgg
    21361 aaaaatattt tttagaaatg attttgatga gatgtcagaa cagggacttc cattttattc
    21421 aggaaccagt gataaaatgc tgcaattaat cactgcaagt aatctatact ggggatttaa
    21481 gcataccttg caatcatatt ttgatggttt aaatagattg ttcatcagtg atattggtga
    21541 ttttattcaa tggtttatta ttatcgttgc tataatgtta ttgataataa caggggcact
    21601 tatgtgaaaa agattaatta caaacatttg aaacgttcat tatgggcata tcatatgaat
    21661 accggttcat gcaacgcatg tgatattgaa atacttgcta tgttaatgcc aaggtatgat
    21721 gctgaaaggt ttggcattaa attagtaggt agtccccgac atgcggatat attattagta
    21781 actggaacgg taacggagaa aattaaagat aaaatactgc aggtatacca gcaggttcct
    21841 aaaccaaagc tggtcatgat cataggcagt tgtggtgcta ccaaaggagt atttcaggaa
    21901 tcatatgcta tggctggccc atttgataca ttgctacctg tagatgttta tgttcctggt
    21961 tgtccgccac gtcctgatgc aataatcttt ggggttgcaa aagcctggga aaagttagaa
    22021 gtgttgtaaa aatagttcat gtggtaaatg gtatggatgt tcaatcaatt gtgacgatta
    22081 ttcaggaaaa attagggaat cacctccagg cttcaaggtt gaaggtgaaa aactaccatg
    22141 gtggaattat gcgattgcac ctatggatca gtatagatcg agaggcattc cattttacgg
    22201 taaatactct aaaaagttta gggaggcttc acctatctac agcaatggct tataaggaat
    22261 atgaagatcg tattgaactt atctatccat ttgccctgaa aatggatgat actcactatt
    22321 ctgaattaat gattattgca tctgtagatt tacctaataa tgatttaaca atcagaacta
    22381 ttaccgatat tgtcccggga gctcttttta tggaaaggga agttatggaa atgctaggga
    22441 tagtaattga agatatccct gatagcagga ggctatttac tccttcatcc ttacaggatg
    22501 gatatttccc tcttcgtaat ggcaaaaaca aggaagatag caaatgaaat ctgatagagt
    22561 atatccttta aatataggac cgatacatcc tgcatttaag gaaccaataa aatttacatt
    22621 caggatagaa ggagagcagg ttactggtgt tgatatagat tttggttata cacatcgagg
    22681 gatcgaaaaa atagctcttg aaagaaatta cattcagatt atctatttac tggagcgagt
    22741 atgtggtata tgttcatttt cgcatccact ggcatattgt cttgctatag aagatgttgc
    22801 tgaaatacag gttcctgata gagctcgata tatccgcacc ataataggtg agatggagcg
    22861 attacattct catttgctgt ggacaggtgt tgcagctcat gaaattggtt ttgatacgct
    22921 ttttatgtat acatggaata ttcgtgaaaa ggtattggat tgtcttgaag aacttactgg
    22981 caaccgtata aattatggga tgctcaccat aggtggtgtc aggagggatg taacaccaga
    23041 gaattcagaa actattacac gcatgttaga atattatatg gaattgtatg atcgtgcttc
    23101 ggaaatactt ctggatgatt taacattacg tgcacgaact gaaggtattg ggatcttatc
    23161 ccgtgagcag gcagaaatgt tttgtgcagt tggccctact gcaagaggtt caggattact
    23221 gaaagatgtc cgtatagatt atcctgttaa tgcttactat gatatcccat ggttaaatcc
    23281 tatatcacca cgtgatcttg gcaaagaacc tattggtgat gtgtatgata gaatggcagt
    23341 acgagtgctg gagattaaac aatcattaaa tataatacag ttttgcatgg aaaacttacc
    23401 agaaggagat attcattcag gtgatggagc agttaaaatt atcaacacgt tgaagaaact
    23461 tgaaggtgag ggtatagggc ggtatgaagc cccacgaggt gaggtatgcc attatgttat
    23521 actggataac caggagcacc ccatacagtt aaaagtcaaa gcgcctacct attcaaatgg
    23581 atttacatgg atacctatgc tgacaaatat tgagatagct gatataccaa tagttattgc
    23641 ttcaatagat ccatgcgtgg catgtgcaga tagaatgact tttattagaa aaggtggtga
    23701 tgaagaaagg tacaactggg aataccttca tcaaaaaagc attaagaaat acaagaggtc
    23761 agtaaatcaa tgattatggt acttgccata gctctcatca ttacgatagt tggattaata
    23821 ttaggacttt tgttgaaggg gatagatcgg aaaatagcag catatttcca gtcaagaatt
    23881 gggccacccc ttttacagcc agtttatgat atcattaaat tggtgtataa acaaactatt
    23941 ataccggtaa cagcggtgag gtggttgttt tcatctgctc ctgtaatagc tctggcatca
    24001 agtgtattgc tactattata tatttcgatg ccatatatat tctacatcac tggcattgat
    24061 actactttgc agggtgattt aattgtagta ttgtattgta ttcttatccc ttctatagca
    24121 atagttactg gtgctttttc gtctggttca ccgtatgctg cgattggtgc tcagcgtgaa
    24181 atggtaatac ttatgagtat agaattacca atagctgtag taataattag tgttgcatgg
    24241 catgtagcag atagtatggc tgatcctttt gctatgtcat cattatttga atatccacta
    24301 tggcataatg ctggtgcatt tggggtattg ggtggcatat tattgttttt tgctatggag
    24361 cttgttttgc cagctgaaat gtccagagtg ccttttgatc aggccgaggc tgaaactgaa
    24421 cttgcagaag gtgtttcagt tgaatattcg ggtaagctac tggcatttat actattaact
    24481 gatgctttta aagcattagc actttcaatg cttgttataa tacttttttt cccatatacg
    24541 ctgtcccaat tgtttggtat atcttttcag atgtatggac atagagtggc cccttttgtt
    24601 gaaatagcat ttttattatt taagattata gtgatttaca gttttgggat aacctttatc
    24661 cgtgttatta tggcacgttt aaaaatatca caggttgcgc gtttttttct ttttgcaata
    24721 acagcaatca gtattattgg gttaatactt attatgcttg atccaaatat gaggattata
    24781 tagtaggtgt gccatgttag gtatggtata taaagtcatt gaacagatct tttcaaaaaa
    24841 atccaccaat tgttttccta cgaaatatat tcccaataat actaaacaat accttaacca
    24901 gggaataata catccaccaa ttgtcgttaa agaaggattc agaggcaggc tggaatacga
    24961 ttttcagagt tgtacaggct gtggtttatg cagtaaagta tgtccagcta atgcaattga
    25021 attatattct gctgaagtta acaataagaa gacaaaaagg atagtcatct atttaacgcg
    25081 atgtaccttt tgtaaggagt gtgttgatat ttgccctaga aattctataa ccatgacaaa
    25141 ggcattctgc atggcagatt ataaaaaata tggtaattca ctggtggttg ggtcggaata
    25201 ccgacagcaa tgtgagataa aggatcatac tgatgttgtg caaaaaaaga caaattgaaa
    25261 tatataaaaa gctacataat ggtgatgaga tatattgttt cagaccatcg ggtgggtgtg
    25321 gaaaaactta aagtaaatag tcatcatccc aatggctcaa tgaatgaaaa atatttgtct
    25381 gggctccttt gattgtttta ttaagatttt ttaaaagttc tttcatttga tatcgttttg
    25441 tgcaatcttt gtaaggacat tcattttcaa taggtgatag tggaagggct tttatataac
    25501 ctttgatctg tgcttcggtt aaaaaagcca gaggcctgat gatttccaat gcaccgttga
    25561 acatttttaa ttttggtggc atggtcgata gttttccatg ataaagcata tttaaaaaaa
    25621 aggttgttat tatatcatct tgattatgcc caaacgctat ctttttgcag ttgagctgtg
    25681 ctgctgtttc aaaaagagct tttctccggt accatgaaca aacaaagcac ggggatgacc
    25741 ctttgataga aagatcaagt gtaatgtctt taattatcag gggtatatta tatattgaac
    25801 agtatgattg aagaatattt attggtttat tattaaaatt tgtcacattt acatgacagc
    25861 aatatagagt ataatgaata ggtaaatacg atagccggcg atataaggca tccatgcaaa
    25921 ctaatgaatc tttaccgcct gataatgcta tgcaaatagt atcgcctgct tctatcattg
    25981 tatacgtgtt gattgctttg ccaacagttt tgacatattt ggtaatttgc ttttctttca
    26041 ttttgatgtg cataataaag ccttgacgta aatatagtta tatggttatt agtagtatga
    26101 gtagattgca ttgtcaatag taattgtgtg taatattaat tgttattgag gttatgaaaa
    26161 tggcgcgctg tatgatagtt gatgattcaa gttttatgcg tcgaattatt catcaaacat
    26221 tagaggaggc aggtcatcag gttgttgcgg aggttgataa tggtttagat gcattgtcaa
    26281 agtataaaga agtgaaaccc gatgttgtta ccatggatat taccatgtgg gggaaggatg
    26341 gattagaagc tatttcagaa attaccagtg attatcctga tgctaaaatc atcataatat
    26401 cagcattgaa tgaaagaact ttaaaaataa atgagaaaga tattaatgct aggctatttt
    26461 tatcaaaacc ttttgaaaag caggaactat tagatgcaat agaaaaagtc ttaaacaatg
    26521 gatgaacaac ttttacaatt atataaaaaa aatgctattg ctgataatgc atatatgatt
    26581 gctgatggta cagtattttt atttgtcaca gaagctgata aatatgtttt gcaggggaag
    26641 aatgtaatta taggtgccac ggaaccagta ttaaacagca aaacgtatga aaatatacac
    26701 agacttgaaa cagccttagc tgtcaaaggt acaacaataa aaaaaattcc tgctaatact
    26761 attatacaga gtttaaataa tactgctttt accatgaatc tggctatagt tgaagctaaa
    26821 gttgttgtat tgacaaatga cattttgaag aaaaatatgc aaatcgattc tcaactggtg
    26881 aaagaacgga atatatgtat tgaatattac actattgtgc attcactgca actggaatat
    26941 gaaaaaagaa aactgccatg gttaaaagat ctaattgata aatttaaaac cagcataaca
    27001 tttaaaaaag gtgaagcatt ttcgcgggca aacgaatcat tacgaattga tactcccagg
    27061 gaactatctg caaatatgat agagtttcct cctggttcgc tattatgtga agaaggttcg
    27121 gtgggtgatg caatgtatat attagagtca gggcaattag aggtttatat acaaaattca
    27181 aaagtggcaa cgatatctga aaaaagtact gttatcggtg agattgcaat gctgctttca
    27241 atgcacagaa cagccacatt gaaagcacaa aatacagttg ttgttactaa aataacacgg
    27301 gaagatttaa gaaggtcaac ttctccagag ttgacgcaag cattgctggt ttcgttagct
    27361 aaaaaacatc atgcaaatgt gttgcatatt atggatataa atgaaaaaaa agctcttgct
    27421 tatgcagact cccccagaca agagctcatc aaaactgaag catatagata tcaaaatgag
    27481 ctatcacaac ttaaatatac actggaaaaa ttaattcaga gcaaacaagc tgattatctg
    27541 gaatatattt taaacaaata aaacttactg ttcaacctct gttaatactt cgcccttttt
    27601 aagggatttc agccagttta attcagcttt gagatgattt tctagatagg tataaacgtg
    27661 taactgagag tttggtgaat tattttttaa ttcagtatca attttttctt ttaatgcatt
    27721 catcttatct tttatatgct caacacgatc ttctatgaat tgttcttttt gttcaggaga
    27781 aagagaattc aggaacatca atacgatatc aatatcaaaa tgtatgaaat tatggtcaaa
    27841 atattgtttc agcatctttt tatagtattt tcgtccgtca ggcagtattt gatatatgta
    27901 ccgctcagga ttacctcctt ccttttcggt acctattttt tttacccagc cattatctaa
    27961 tgctttctta attgcataat aaattgaacc aaatttaata tctacataat cttcaaggcg
    28021 ttcaactata cgctttttaa tttcatagcc ataaagattt tcttccatca ataatcctaa
    28081 tatggttatt tcaattttca tgatgcacct cgtttgaata aatttttatt ataaaattta
    28141 taatataaaa aatacttata aaaatcaatg ataatattgt atgttcataa aaaaaattta
    28201 caaatagttt aaattagatt atataaataa gacttaatat ttcattatgt ttaatatagt
    28261 ttaattttac tattattcag gtgtttaagc atttatcatt cgatttcttt caataaagaa
    28321 ctgtaatgaa taacaattgt atatcaaatt ttaactcctc ggaaaatata taatatggac
    28381 tcctgtcatt ccgggcttta accggaatcg caatatgtaa ctgtaaattc cgaaatgagt
    28441 tcaggatgac tgctggcaac tatttttttc attccctctc agtgtcagca tgctgaccat
    28501 catgaactct ctttaaataa caagcaaaaa caagcaaaat ttacaacact aatcatgcaa
    28561 tactgtagaa cttccaatca tgataaatca atatccatga ttattgcgat aattgtttca
    28621 atgataatcg gattattgtc tctaaatcac aatcaggatt gcttgtgata attgaagaaa
    28681 tagtagacat tgcaagtttt tcatcaaagc ctaatgacac caatgcatcg atagcgtcct
    28741 gtctatcggg ggatgttgta gcaggatgct gaacagtggc ttctaatttc cgaagtttgc
    28801 gttttagttc aaagattatt ttttcgcttt tactttttcc aattcctggt atagcggtaa
    28861 aggcaagaat attttcagct ttcacggcgt tggtaatata gtgaggcgac atagatgata
    28921 aaattgatat tgcaatggat gggcctaccc cgggaattga tgtaaggatt gcaaacatct
    28981 gcttttcttc ttctgaacta aagccataca gggcaacgga gttttctctg actaccatag
    29041 caatataaag ctcgatagta gctacagaac ttattttttc ataggtagat agggggatgg
    29101 taacctcgta ccctacccca ttaacatcaa cgataacttt tgttggttta atagatattt
    29161 ttttgccttt aagaaatgca atcatataaa actcaaaatt gaaataaatt tatacttttt
    29221 ggtgtaataa tgatctttct ggagttgcca gacaatgaca tagtgctatt gcaattgcat
    29281 caaatgcatc atcataatta tgtattgtat cgcattttaa aatttttctc agcatggtcg
    29341 atagttgctc ttttgaagat cttccatatc cggtaataga ttgttttatc tgcattggtg
    29401 tatattcaga ataaggtata gcatgctgag ttagtattaa cttacaaaca cctatgacct
    29461 gcactaccga tagagcagtt ttagtatttt tactgaaaaa caatttttcc aatccacacg
    29521 attgaggctg atataaagca attaaatcac ctacactttg atatatagta aaaagtcttt
    29581 caccttcgtc accaggagtt gtttcaataa caccacagtc aatataggtt aatctatcgg
    29641 ttactatcgc ccaaccacag cgtccataac cggggtcaat tcccattata gttgccacat
    29701 cgattagctc atactattaa taatatcatc cgggatatca aaattggcgt atacatcctg
    29761 tacgtcatca tgatcttcaa gagcttcgat caatcgtaaa cattgtgatg ctttttgccc
    29821 ttccagattg atggttgttt tgggaatata ggttatttca ctatttaatg ttggcagatt
    29881 gtgagaacgt atgatttcta atatgtcatt aaatttatca ggagaggtat agacaacaat
    29941 ggtagtatct tcagttttta tatcctcaac atcataatca aggaggattt ccataacctt
    30001 atcctcattt gttgcatttg catcaatgtt aatgacgcct tttctaataa aaaggaatga
    30061 aacacatcca gattcgccta aatttccgcc attttttgaa aatatagtgc gtatctctgg
    30121 cgttgttcta tttttattgt cagtcatgca gtcaaccatg atggcaaccc caccggggcc
    30181 gtacccttca tagcgtactt cttcgtatgt tgaaccatca agagctcccg tccctttttt
    30241 tattgcccgt tcaatgtttt ccattggcat attgttttca cgcgctttta aaacagcagt
    30301 ccgtaaacga ggattgctat tgaggtcatc accaccgacg cgagcggcaa cggtgatttc
    30361 ccgtataaat ttggtaaata tttttccctt ttttgcatcc tgtgcagctt ttctgtgctt
    30421 aatattagcc catttagagt gccctgacat aatgaatgct cctcacaata caatttgtaa
    30481 acatgaagta aaaactaaag taatgacgaa tgttagaaat gtcattgatt ttgtcattaa
    30541 taaaataata catcttttca ataaaaaaat ttttattaaa atcctctgga aatttttagc
    30601 tgtaattttt caattttttt gctggattct cttattttaa ttccatactc aatggttttg
    30661 ttataccatt caattgcttg cgcaaattgc atcatattct catgggcgac agcaatttta
    30721 aacgatagta atcctgcatt caatttgctg tattcatgtt caagctgccg taattcaagt
    30781 tcattgtttc ctaattcatc cattagcgtt tgatattggg ggtataatgc aatatcttta
    30841 ttagcaagca atgttgattt aattacgttt attttatcat tgattgtatt aatattggct
    30901 tttttttcat ctattatttt tttcatattt tccgatatgg ctattgactg tagatagtat
    30961 tctaaaccat ttttgtaatt aagatccata cattcaatat cacctaacat tgtataagat
    31021 agtcctttta atacccaatc attttcattg aaaagtggga tagcatttat aaatgatttg
    31081 tatgctaact tcatatccga aatttttgta taagcaaggt agcctaatgc aaagagaaca
    31141 gcgtaatcat tgggtttatg atgaaggtat aatgtatatt gttcggatgc tttgatgtaa
    31201 tctcctattg attcatagca gtttgcagcg atcagataga gattgggggg aacactatca
    31261 ccttttgatg caatataacg gttgagatga aataaagctt tctttttatt cttgccagat
    31321 gaaagagcta aggatagttc taaagaataa tcataattat ctggtttaat ttcatacgct
    31381 tcctctaaat agagcacata cgcattaaat ttatttattt ttttaaaaat ttgtgctatt
    31441 gaataaaaga ttgtatgatc cttttctcca attgttgtaa agcgtaatgc ctgaaaataa
    31501 tgctgtaatg cttcatgata tttgtagatg ttttgataac aaagtgctgc atcagcatgt
    31561 aatgcataga gggtatcgtt attttctata aaatatggct gtatctttct ttctgaaaaa
    31621 atcttttgta aatcaacccc ttcttcaatc ttttcaatgg caacataggt ggttggtatc
    31681 tgcttttcaa tggatctttt tgaatatata tcgtcaagct tggattggat aattgtcaaa
    31741 gcttcagaga attttttttg gtgtatagca tctttataat tctgtccaaa caggtagctg
    31801 ttcatgaaaa atgataacaa taatgtaatt aaaattggta gtttggtatt catagtggtt
    31861 attaggtata cttttattga tattatcgga tgagaaagga agtaagttaa aaggaaatgc
    31921 ttatctacgg atacgtatcc tgggcaattg ccgtttttta attttcagcg caccttcagg
    31981 attatatttg ttttgtattt cttcgatact gtggctaatg agcattcgta ctacctcctc
    32041 ggctttattg agaacatcta ttaagataaa attttcatcg tctgagaaat catttaacag
    32101 atgttcttcc atcgttatgc ctttatgtgg gtagccaata ccaattcgta cttttgcaaa
    32161 tctatcggat ttcaaagcct ttgtcataga ttctattcca gggtgcttga gcttggacat
    32221 aatggaatca acacgaattt cgcccagagg caaagacgaa tcttccagga cacagataat
    32281 atcacggaca ttgatacgta aaaaagaagc aatatacagc actgactcac caataagggt
    32341 aacaaaggtt tgaggtttca acaaaacgac atcctcacct gaaattttgc cacgtccaat
    32401 gatagatttt ttctttttga cacgtatgtc aatattctcg ttattgccaa gaatatcgac
    32461 aaccttaaag ccaatgtttt gcctgttatt gaagtattct tcgcctggat ttccaaatcc
    32521 tacgattaat ttcaattaca tacacctcat tactaaataa ttactgttct tcaacagttt
    32581 cttgaggttg ttccccagct ggttgtgcaa cctcttcggt ggctttatgt ggtgcaagta
    32641 cagctacaat aaccgattca ggtgaactta ggatcttaac accgtttgga gctataatat
    32701 cctttacatg tatagattgc ccgattgcta aatttgttac atcaatttga accttttcag
    32761 gtaaatctgc tggtaggcac tcaacttcaa tttcgcgctc aattacttct aaaatcccac
    32821 cctgcttaac accaatagaa gttccaataa tttctatagg tgctttggta gttatggtct
    32881 cggtcattgt tactttaaaa aaatcaaggt gtaaaatttc atccgttata gggtgtcgtt
    32941 gataatcctt tataaaagct tttaccgggg cactggaatg atcattaaat tggaggtcaa
    33001 tgagcagatt ttctgatata tgtcccttga agattttaaa gaaattcttc ttctcaacca
    33061 ttacagcctg tgatacgcca tgggagtata gaacagcagg gatgtatccc tgtgctctga
    33121 gcctatgatt ggcatttttc ccaatttctg atctcgtttt aattggtaat acttgtgctt
    33181 ccatatcaac ctcttccaga taatttaata tacattatag taaaaatggt ataaaattca
    33241 ataattattt actatgacat tatcgtataa ataatgaact taccgattct ccattatgaa
    33301 ttcttcgtat tgcctctcca aaaagtggtg ccatagatag tattttcata ttgggtaata
    33361 ttttatgttc agggatatct attgtattgg ttaatatgat ttctttaaat tcagcctttt
    33421 taagtttttc gatagcattg tcggataaca ctgcatgcgt ggcagtacaa taaatatctt
    33481 ttgctccact cttctttaag gcatttgctg cttgtgctat agaacctgct gtatcaatca
    33541 tatcatcaat gataatgcag ttgtgatttt taacgctttc ctcgccaatg atattcatca
    33601 cctccgattt gtttggttct ggtcttcgct tgtcaacgat tgctaattcg gcactgatat
    33661 tacgagctaa aaatcgagca cgttcagttc cacctgcatc aggagaaacc actgttggat
    33721 tttgtatatt caattcattg acatattcaa taacaactgg agatgcaaag agattatcta
    33781 cggggatatc aaaaaaaccc tgaatttgat cggaatgtaa ctccatggta agaacacggt
    33841 tgacacctgc agcatggagc atgttagcta taagcttggc agagatagga acacgcggtt
    33901 caacttttcg gtcctgtcgt gcataaccat aataagggat aactgcagta atacgctgag
    33961 cagaagcacg cattgcggca tctaccatca acagcaattc cattatgtta tcattggcag
    34021 gattgctggt agattgtatt aaaaaaacat caacgctgcg aacattctcc agaattttaa
    34081 cagatatttc accatcttta aattttttta tttcaatttc agaaagctca atacctaaat
    34141 aatgagctat atcctgagct aatgcataat ttgatgttcc cgagaataat ttaattggat
    34201 aaatcatata caccctattt tttaataaac gtttttgtaa ttatgcttcc aagttttata
    34261 agttcttctt tagaatttac accagttcct tcaatggggt catcaagttt aatgattttc
    34321 acatcatatc cagactttct gataaatgtt aaaacatcag gtagataata ttccctttga
    34381 gcattgtcgg tggtaatagt ttttaacccc tggaaaagaa taggactttt aaaagcatag
    34441 gtaccggtat tcacttcttt tatattcctc tgaataggat ctgcatcttt ttcttctacg
    34501 atcctaatca aattatcata ttcacctcgg ataatacgac cgtatccgca gggattttca
    34561 agttccattg tcagaactgc agcacctgta ctgtggtcgt taacagcatt atacagcttg
    34621 ataaatgttt caggctttat gagtggcaca tcaccgcatg caacaagtaa acaaccgtta
    34681 taggtgccca attgaggttc agtttgcatt acagcatgac ctgttcctaa ttgttctatc
    34741 tgccagacaa aggagatatt gttgtcaatt gctgaaatta catcatcacc tttatagccc
    34801 acgacaacta taatatcatt ataacttata cctgcactga tgagattatc tataacatgc
    34861 tgtacaagcg gtttcccggc taacctgtgc agtaccttgg gcaggctgga ttgcatgcgc
    34921 acgcctttac ctgcagctaa tatgacagct ttacagttat tcataactat cactcataat
    34981 aaagtatgct gaggtgccag gattcgaacc tgggaatgcc gggaccaaaa cccggtgccg
    35041 taccacttgg ctacacctca aaataatatt ataattatag taataaaatg ccttatgtca
    35101 taataaattt gatacaaata cttgtgaata gtgaggttta agcgtcagga aaacctcatt
    35161 ggcttttcca atatcgtgaa aaagtgcaca taatgccgaa ccgctacctg tcatatggat
    35221 aaaatccggg gcaaaatcct gcaaggtgcg gtaaatttcc ctaagctgtg gattgttatc
    35281 aaaaacaata tcctggaaat catttttaaa aatgtttaat aaagcaccta catcactttt
    35341 atcccataaa gacaaaactg cttctttttt tgacaaaaca ggcgcaaaac ttctcatcaa
    35401 tgcggtgtat gcttctttgg tactgctgtg aacgtcatta aaaacaataa caactgcatc
    35461 tggcagaggt atttttttca atggagttac tatttcacct ttaccttcgc aaagagcaat
    35521 atttccattt aaacaaaaag gaacatcagc tccaatctca gcagctaacg ccataagttc
    35581 attgtgactg aatttattga atacgttatt acagagcatc aaggctgctg cagcatctgc
    35641 acttccgcca cccaatcctg cacctgatgg aatttctttt tgtatggcga tagtaaccct
    35701 tgagccaata tgtgcagttg aacaatataa acgtattgct tttgtgatca ggttttgctc
    35761 tatggggagt tcttttacta tagcggcatt ttgcccgcct atgcatcgaa tagttatatc
    35821 gatagtatta ggattgcata gttcacatcc ctcaagttgt aaaagatcaa agatattaat
    35881 cttcgccatg atactacaga tattatggta gctatctggc cttttgttca aaacttctag
    35941 atgaagattt atttttgcgt aggcttttat actttcaatc atcaataatg gtattgtgta
    36001 atagattggc aaatctatat tttagctgtt tttctgtcaa gcaattaatg ttgtattaac
    36061 aatatagttg gtgcaggttc aagtttagca ggaaagatgt atgattgtag atcaagaata
    36121 attaattata tataaaatta caacttacaa caaaacacat ttgaagccta atgttaatca
    36181 tttccatgtt ataattatga cattttgtaa taataattga caagaatacc gctatcttgt
    36241 attttatttt aacatagtaa cctttaataa ttagttttga acattccctt ccggggataa
    36301 gtattggaat actgtgtatc gtattggtgg taatgaaaag ctggattccc agaggttccc
    36361 ataagcaaat agatatgagt taaataaaac aatgaagaat agtatcataa ctacccatag
    36421 tttaaatggc agtatttttt cttgtattaa aaatattcat ggactaagtg cacaaggatg
    36481 aagctacttt tttcataacg tagtacgcta ttatgaatat caaaatttat gttagcgcgt
    36541 tggctatgaa attatacctc attccactat tagtagcctg tttattcttt gcaggttgct
    36601 ctccgacaga ggtatttttt tcaagttatt ctgcacaatg tggagtattt gattgttcta
    36661 caattgattt tactaaagag aagcctgttg agcttaaggg tgagtggaag tatagaatgc
    36721 tggaagatag tccggctttt gctcgaccag attacgatga ttctgaatgg gattatatca
    36781 taatacccaa ttactggaat tctgaaaaat ttaaaggcga tggctatggc tggtatcgct
    36841 taaaggtaaa agtaaataaa agtttttggg aaacttcacc agccatctat gttccaggtg
    36901 caaacacagc ctatgaactc tatttcaatg gcaacaaatt tatgtgttct gggattgtta
    36961 gcattgatga gcaatcctca agcccttgtt taaaacctca attaaaacct ttaaactgtg
    37021 aaaaagaaac tgaagaattt gtcatagcag taaaagtatc aaactttttt catcgtggag
    37081 gcggccttaa taagtgtata gttattggtc cttttgaaag tattacacag attctgtggg
    37141 aacatgattt tattaaaacg gttatacttg gcattatatt catcattgct gtctatcatt
    37201 taattctctg gatggggcgt agaaaagata aaacctcttt ttggttttcc atattttgtt
    37261 ttgctatttt cagccattca tttgttatag gtaattatct tgaacgaatt ttttataatt
    37321 ccgatgtgtt ttttatcaga ttttcaattg aatatggtag ccttggttta ggatggttaa
    37381 gttttacgct ctatgtttgt tatcttttcc ctcgtgaatc aaacagaatg tttgtaattt
    37441 ttcagattat tacagggatt atttttataa gcattccaat gattttaccc cctaaaatat
    37501 ttacaaattt gataggtgct tataatgtaa tactgttcac tacaatgatt ggagccttta
    37561 taattcttat tgctgccagt attcgtcgca gggatgatgc attgttaata tttatagggt
    37621 atatagtatt aatgataact gttgctaatg atattttaca taatctattt atcatacgta
    37681 cattttttat gtctgaatat gggcttgcgc tttttattct gttccaatca tcagttttgt
    37741 cattacgatt tgcaagagca tttaagacaa cagaatatct ttcagagaat ttaaatgctg
    37801 aagtaaagaa aaagacggaa gagcttttga atcagactga aaaagccata tctgctcagc
    37861 aggatacaga gaaaacgtat aaaaagctgg catcattata tgatcaaata tcaaatgaag
    37921 ttcatcttgc aaaaactatt catgatagaa ttattcctaa agaaataatg cacataccgg
    37981 acgtaaagat tgcagtgtac aatgaaccgt ttatgcaaat aggtggtgac gtttatgata
    38041 tagcttctct accaaataaa aagacacgcg tgtttcttgc cgatgcaact ggacatggta
    38101 taagcgcatc attgattacc atattgataa aagttgaata tgataagtta aaggatgaat
    38161 gtgaaacccc tgctgaagtt tttaagaaac tcaatacagt attttcggat tattataaaa
    38221 caatgaaaat gtttttaacg gcaattatgg ttgatattga tgggaacaat aataaacttc
    38281 tttatgcttc ggcaggtcat cctgatcaat ttttggttat gaacagcact atttttgagt
    38341 tatcccgcac gggtcctgca attggaattt caaaattttc aaattttgaa aataagataa
    38401 ttgatatcaa aaaaaactat aaattgtacc tatttactga tggtataatt gaggaattta
    38461 gtacagacta ttttggaaac caatataaag atttaaagca ttttatactt gaaaatgggc
    38521 acttaagtgt tgaacaaaca gtatcgctta ttaatgaagc gataaaaagg aatgcatcaa
    38581 acattgaaca ccgtgatgat aagattttta tagggattga gtctatataa aataaaaaaa
    38641 gttactaaaa ggcaatgacc cgataagcaa agaatatggg tgaagtgatt gaaataataa
    38701 taatccatca tgaataggca taaatttttt ttccagataa taataaataa tcaggtattg
    38761 atttattttt gtttttatag tatattatat gaccatgaat agtaatacag tattttagag
    38821 gatgtttaat atgaataaaa aagtattact ggcaataaaa aataataatc gggaagctat
    38881 tacaaaactg ataacaagta caccaggtat tagtggtgta aactataaca ataaacacta
    38941 tgaaattgag tacgatagct ccgttataag tcccatacac attgttgaac tgctggaaga
    39001 aaatggagta tacatagaaa aagaaattgt acaggcaaca ataggtgaca tgagctgtgt
    39061 catgtgctct aaagcggtaa cgaatgcgat tatggatata gagggtgtgc ttgatattaa
    39121 tgtaaactat gccacaggga aagcaatgct tgttgttacc cctgaagctg ttcatgacag
    39181 cgacataaaa acaaaggttg aagatgctgg ttacaggttt ttagggacag aagaggtcca
    39241 aacacaaaaa cagtcaccac tgcgcctgat acagattgtt gctgggctgg tagctggtgc
    39301 tttgcttatg gcaatgatgt atggattgtg ggctatggat actattggca tcgctattat
    39361 attaacgcca gtatttcttt ttgtgagcta tcacatattt actggtgcat accatgcact
    39421 gaaaaatagg gttttatcaa tggatgttat gtatgccatg ggcatcgggg tatcatttgg
    39481 tgcaagtatc cttgcttcac tgcacgtgct gtcgcatgaa tttatgatct atgaaacctc
    39541 aatatttctg gcatcatttc ttaacatcgg gaaatatctt gaggaaaggg ctaaatcaag
    39601 gacatcccat accattgcaa agctactatc gcttagtccg gatacagcga tagtcattcg
    39661 agatgggaat gaaatagaaa taaacaccag tgatgttacc attggagaca cggttgttgt
    39721 gaaaccgcaa acgcgcattc cggtagatgg cgaaattgta aagggacaaa gttatgtgga
    39781 cgagtcgatg gtaaccggtg aatctgtgcc agtattcaaa agcgaaggca atgcggttat
    39841 tggggggaca cttaacaaaa acagcctgat tgtgataaag gcaaaagcta ttggacagga
    39901 ttcaatacta tcacggatta ttaatctggt tgaacatgca gtgatgtcaa aaccacgtgt
    39961 gcaacgtttt gccgacaggg tggtaagcat ttttataccg gttatactga cgattgcaac
    40021 aatagccgca ctggtttggt atgtaataaa tggggatata tttttttcat ttgaggtgtt
    40081 tgtggctgtc attgttatag cctgcccatg tgcattgggg cttgctactc caactgctat
    40141 aaccgttggt attggaaaag gcgctgaact gggaatactg attaaaaata gtgaagccct
    40201 ggaaaatgct tcaaaggtta cgcatgcagt atttgataaa acgggaacgt taacaaccgg
    40261 caccatgaca gtgaccaata ttatagcgtt tgacaacatc agtgaacatg aactgctatc
    40321 ggtttgtgct tccctggaac gctatgctga gcatcctgtt ggtgaagcaa ttgtacaata
    40381 tgctaccaat caccatgcag tgttccatga ggttgaggat gtcagcgtag tggaagggaa
    40441 aggtgtcatg gggacgatag ctcaaaaaaa ggttattgct ggcagcagag attttataca
    40501 ggattacata ccatgtgaag taactatcgc acaaaagatg aaagaatttg aaaatgaagg
    40561 aaaaacggtc gttgttgtag cacaggaaaa gccgtatggt attattgcac tatcggatac
    40621 gcccaggaca gatgcaaaca cggttatacg ctctttgcaa cagcgtggta taaaaacagc
    40681 catgcttacg ggtgataaca ggctgagtgc tctggcgata gctcatgaaa ttggcatgga
    40741 tgctgtatat gctgatgtta aacctgaaga taaaatgaat gttgtgaaac agttgcaaaa
    40801 ggatggagag attgtagcgt ttattggtga tggcatcaat gatgcgccgg tgctggcaca
    40861 ggctgatgtt gggattgcac ttggcagtgg aactgatgtg gcgatagaag ctggtgatgt
    40921 agtactcttg aggaacagat tgcgtgatgt ggtgacctat tttgagttag cagtaaaagt
    40981 aatgcgacgc ataaaacaga atatcttctg ggcatttgca tataatgcgg ttttagtgcc
    41041 tgtagctgct ggcgtgttgt atcctttcac aggctggctg gtaaaacccg aactggcagg
    41101 ggctgctatg gctttaagtt cggtaacagt ggtaagttta tcgttgatgc tcaagaggtt
    41161 taaacctggt atataagctt catcagtgtt tcttttatcc atgctggtaa ttcgccataa
    41221 tcctcaaagc tcaataaagc ccctaaatat attttataac tttgtccctt gaacttcttt
    41281 tcaaaacctt gtataatgta gttgttatcc caattatgct ggttatacaa ataagcagca
    41341 tcaaaaggat ctttggggtc aattcttctt cctgcaacat acagtttgtt caaaaagaga
    41401 tcaaaatcac ctgctacttt aatgtgatga atagtttcaa ttttttcaag gtttttaaaa
    41461 ggaaaaaaaa caaatgatac ttttattcca ttgataaaga aatctatatt atcctcttta
    41521 cgctcccatt tatcaatctg gttttgatat aatggtatta ctttttttgc caatcggtta
    41581 tatgaaaatt ctttaaaatt aaagaaatca agatcataac ttatccggtg gttatatttt
    41641 aacattatgg cagtaccacc agctaaataa aaatgcggaa actgctcttg tatctgttgt
    41701 gccagggtaa ttattgttgt gttaatggtg ttcattccag taccgcaccc agaattttac
    41761 tccccgtttt atgtcagggt aacgagatac tatgtcagtt gtttccgttg aatataaaga
    41821 gtagatagta tgtatttctt cataattgcc atactgcaaa acacggagaa ttaatttttc
    41881 aagtggagca gtatcctgtt catcccataa aagagatgta aggttagatg gaatgggtac
    41941 tttttgtgag tagaatggtt ttatacgttg tggtttttga ataccactgt tctgttgctg
    42001 catgatagtt acctgataaa aaacaaatgt aaagtgtata ctgtaaatac agtatcatgt
    42061 ctatagtaat aaagcaacta ttttatgtat tacacatgat tacatattaa aatttacaaa
    42121 tttcagattc atgggtgtta ataaaaaatg acattattac cggttcagta aagcgcggct
    42181 attttgaaaa tatgatatgc aatgaaataa caaagaggat tattccaaaa atacggcgga
    42241 gtgtttcgct atcaagtgac accgcaatct taccaccaaa atagccccca atgataaaac
    42301 ctaaagccag ggtaagcgca accggtatat taacatagcc ggatttataa tatgcatatg
    42361 ctgcaagaat accaataggt ggtatcattg cagcaagtgt tgttccctgt gctaaatgct
    42421 gattaaaatg cattacatat atcaatgctg gtatcatgat aatcccacca ccaataccca
    42481 taagtcctga taacatgccg gctgatagcc caataatagt tgttataata agcatacaga
    42541 cctcttttgt taaaataaat gttgaccaga aaagtagaat acaattgctt tatgtcaagc
    42601 gatatactgc aaaggtttag cacatggcaa aaaatacctg tattgttacc tgtgagcatg
    42661 ccggaaacta tataccacct gagcttacag cacttttttc cggagagcat gaaatagtag
    42721 caacccacct tgcgtatgat attggggcat taccaatggc tatgattatt gccagcatac
    42781 tgcatgtgcc acttatatac acaaaggtta cacgccttat tgtcgattgt aaccggtcag
    42841 taggaagtga tgaattattt tcgaaattta caaaaaattt tgatagcgtt cttaaaaatg
    42901 agctgatacg acgttattac gataaacacc gcaatacaat agaacaggct gttagcaggg
    42961 caatgcgaca tcatacgcat gtcattcatg ttgctgtcca ttcgtttaca ccacaattga
    43021 atggatttaa acggtattgt gatataggac ttttatatga tcaatactgc ccggtagaac
    43081 agcaattaag taaaagtatc aaagaaactt tgtttgcact gcatccgttg cgcatagcat
    43141 acaattaccc tttcaggggg tgtggtgacg gtgtaacggt atggcttaga aagcagtacg
    43201 gagataggta ctgcggcata gaagtggagg ttaaccagga tttatatttt cagggaaacc
    43261 atttgtggcg acagttatcc agcaatattg ggcaggcaat aaaaagggca atgacaacag
    43321 tgtaaaggat ttttgctata ccatacacaa taagaaatct gccatccatg ccgagatagg
    43381 gtagcgatga ctattgtttg acagtggtct gagtatcaat actgtttttg ggagaaggat
    43441 ataatggtag tagatatgac gatggcgcag aagaaattaa aaaacataaa ggctgagtat
    43501 gctgaaattc gaatgagtga aagtgatacg gtaagcatcc atctttcaaa gaatagagtg
    43561 gaaacctgta aagctggtaa aactacgcag ggatcggtgc gcgtctataa caggggatgc
    43621 tggggatttg tttcatttac aacactggat atgatagatt atgcaataat ccgtgcacag
    43681 gaactatcgc acatacaaaa aaaagatgac gctgtttcca ttgctgccat gcaatccaat
    43741 gtagtgaaca aaaaaacaaa agtactgaag cctttgcaca gcgtaagcct tgaagaaaag
    43801 atggagctta tgcaccgcta taatgcaatt ttaaagagca gcccattggt tgagaatacc
    43861 aatgttgtat acagggattc catgactcac tattatatgt acaatactga cgaaacaggt
    43921 gttgattatg aaagctcgtt ctgtggcgtt tctttttcgg ctatagcaaa ggatggtact
    43981 gttatgcaac cgtacggtga atcgattgca cagtacggtg gatttgaact ggttgaggat
    44041 aatcacgctg tggcagaaga ggtggtgaaa acggctgcag agctggtaaa ggcagagcag
    44101 gtacaaagtg gactatacaa tgtcatagtg gatccaaagc tggcaggggt gtttattcac
    44161 gaggcatttg ggcatctttc tgaagcagat tttatctacg agaatccccg aatgaaagag
    44221 ctcatggtga ttggcaggcg ttttggtccg gaatgtttac aggtgattga tgatggctca
    44281 ctaccctata cagggtatat ccctatagat gatgagggga tacaaccgca aaaaacctat
    44341 ttgattaagg atggtatttt gactggcaga ttgcactctc gggaaacagc taaaaaaatg
    44401 ggtgagcagc caaccggcaa tgcgcgggca atatcgtgtg gtgcacaccc tattgtacgg
    44461 atgaccaata cctatattga taatggcacc gcaactgctg atgagctgtt tgatcgttta
    44521 ggtaatggat tatatgtagc tggcgcatta ggtgggcaga ccaatctgga gatgtttacc
    44581 tttaccccgg catatgccta tgaggtaaaa cggggaaaga aaacgagatt attgcgcgat
    44641 tgcatgctca gcgggaatgt gttcatgacg cttatgaata ttgattcaat tgcagatgat
    44701 tgtaccatgt ttggtggatt gggaggctgt ggcaagcagg ggcaggcacc acttccggtt
    44761 tcttcaggtg ggccgcacct attgataaaa aatgttctta taggaggcag cggtgctgga
    44821 ggcagcaaaa agaaatcatg atgctattcg caaagaatgc cagggcgttc aatgcagatg
    44881 gtgggatatc tattccaatg atactatcag taagtcagtt acctttaaaa ataatgtatt
    44941 tcattcagcg cgcgaatctg cagtacgggg atatggtatc cgcctgaaca tacatgggaa
    45001 aaccggtttt tcatattgta atgatttcaa tgatataaaa actgtcattg cccatgcaaa
    45061 ggatactgca cagtatgggg ataacgagga ttatgcactc cctggtaaaa atgccattcc
    45121 taaagtaacg ctctatgatg aacaatggtt ggaaagctgg actgtagaaa atcaggtcca
    45181 gcaagtgcaa gctatcatag atacaatccg taaagcatat agtgattgca tggttgatag
    45241 ttcggcaggg tgtgttgacg gatatgttta tttgaccaac tccagtggtt ttgaagcggg
    45301 atatcgttac ctgcaacatg gggttagcgt ttcgggattg cgcatagtat cggatgggag
    45361 ccgtatgcag gtatatgaaa gtacttcatg gggaagcaca tttgattacc atgaaattgt
    45421 taatcgtctt attatgaagt tgcaattcag cgaaacgaat gctgctatcc cttctggcaa
    45481 ctattatgct atactaacgc caaaagcatt tggccagatt atgggggtgg tactacaggg
    45541 gctttccggg aagtcgattc aacggggtat atcgcgctat ataggaaaat ttgggcagaa
    45601 agtttttggc aagcagttga gtgtgtatga taatccgctt gaacattttg cacccggcag
    45661 ttaccctttt gatggtgaag gggtgatggc ttttaataaa acgatcattg agtgtggata
    45721 tgtacaacgg catatagcgc acctgcagag tagccatata ttacagtgca acccaaccgg
    45781 taatgcatcg cgtagctatg ctacattgcc acaggaaagc tttagcaaca ttgtggtgcc
    45841 gggtggggat gtatcattca aagatatgat tagacagatg gacaccggta tcattattga
    45901 ccaattcata ggatttggtc aatcaaatac attgatggga caattttctg ctaatttaga
    45961 tatggcatgg tttgtcaata acggagaggt acagggaagg cttaaaaatt gtatggtggc
    46021 tgatgatgta acaaggatgc ttgatgatac aatagtacta tcaaaagagc gcgaatggat
    46081 tggcgatagt tatctgccat atgttatgtg caggattgat tacaccacca tctaattcac
    46141 tgataatatg aaagttttac aaaatactat aattttattg cttttttaga taacttagtt
    46201 atgtttgcgg taaaattgag aaaaggcata cttattcaaa tttatatata aaggagtatt
    46261 caatgaaaac aagaattaca gaactattta agattcaata tccaatagtg ctttcaggga
    46321 tgagctggat aagcactccg gagcttgttg ctgcagtatc aaatgccggg ggtttgggaa
    46381 ttcttgccac cggggtatta acagcagatc agacccgtga agcggtgcag aaaatacggt
    46441 cgctcaccaa aaagccattt ggtgcaaatg ttacccttta tttccccggt gctgaacgga
    46501 atgcacaggt tttaattgaa gaaaaagttc ctattataaa ttactcatta ggcaaaggtg
    46561 ataagattgc aaaagcagct catgcctatg gtggcaaggt agtggcaaca gttaccacac
    46621 ataaacatgc atttgctgca caacgggatg gtgctgatgc attaattgtt acaggatatg
    46681 aagcagccgg gcatggcgga gcaattactt cgcttgtact tattccggca attgctgatg
    46741 cgttagatat acctattatt gctgctggtg gttttgccga tggtagaggg cttttagctg
    46801 cgctggcgct gggggcagat ggcatagcaa tgggtactcg ttttatgaat accaaagaaa
    46861 gtccggctgc agaacaggca aaacaggcaa gtgtaaaaag tgaggtatat gatactattt
    46921 atacaccaaa agttgatggc ttgcctgcca ggttcatgaa gtctaaaggg gttgagcatt
    46981 tattgaaaaa acgattaaat ccattcagtg cacttatcag atccaaaaag attgccgata
    47041 tgttagagtt tccatggctt aagttagcga ttggcattat gctcatgggg ccaaaacgat
    47101 cgatgcaaat ggcacagatg gcaattggat ttgatgcatt taaactgggt accattgatg
    47161 gcgattttgc aaaaggtgtg ctgccattgg ggcaggtaag cggcataata aaggatacac
    47221 caacggtaaa gcaggtagtg gattcaatag taaaagaagc agtagcgata gagaaacagt
    47281 tatcaaaaaa agtaaaatag cacgatagtc attgaagagt acgacagaat tatcatcaaa
    47341 gaatattatt gcaaaagggt ttgttatgca gaaagcaaca ttgcaaggat tacacgattt
    47401 tttatcagaa gaaaaaacgg ttgtttctat gctactatcc cgtgctgatg cacgaggtga
    47461 tagtatcgca ttaaccggct ttaagggtga tgagaaatat tcctatacat ggtctgagct
    47521 taaacaccat gcctatgcaa taggatattt tttaatatcc catggatgtc agtctgggga
    47581 taagattgca atttttgcgc aaaattgtcc tgaatggacc atagtagatt taggaataca
    47641 atcttccggg tgtgtccctg ttccaatcta tgcaaccaat tcaaaggatg aagctcgata
    47701 tattattgaa gatgcaggaa tcaaggtggt atttactggt gatcaggaac aatttgataa
    47761 gattttagaa attgccacca tgtcatctgt tactcatgtt atatcgctgc aggatgatgt
    47821 cactgtaacc agtgaaaagg gtttttatgt aaaagatatt attaatacta cagtagatag
    47881 tgcggttatt gaacaggtta catcccgcat gcggggacta tcaagtaacg atatgcttac
    47941 cattatttat acctcaggga caaccggcaa tcccaagggg gctgtccata cccatgcaag
    48001 ctttttagca ggtatttatg cttcagtagt gcgatttcct gaagcaggtc cgggtatggt
    48061 atcgctggca tttttgccgt taagtcatgt ttttgaacgt atgtggagtt atggggtact
    48121 ccttaaaggt ggtgaaaacc attattgccg aaatccaaaa gatattatgg caattttacc
    48181 gctttcaaaa ccacattata tgtgtagcgt accgcgtttg tgggaaaaga tgtatgctac
    48241 aatatttgac agattggaat cggcatcacc ggtaaagaaa aaattgtttg tatgggcaac
    48301 aaacgctgga atagaatttg ataccagaaa gcgttcaaat aagtttatca atccattctt
    48361 gctttgcaaa aaagctatag gggagatagt tgttttgaaa aaagtgagag atgtggtggg
    48421 tggtagagcg agtgtgttcc atgttggtgg tgcagcaatg tctgcagata ttaatgaatt
    48481 ttttaacggc ctgggtattc cactggcaca agggtatggc ttaaccgaat ttttccctgt
    48541 tgcagtgggt actgataaaa cagcatatcc cggaatatgc ggtcctgttc ttgatattgt
    48601 tgatgtacga gtttcagatg agggtgagat acaattgaag ggcccaatgt gtatggatgg
    48661 ctactataat aaaccggaag caacaaggga gatgtttaca tctgatggct ggtttaaaac
    48721 aggtgatgtt gggaccgttg aaaaacataa tgataacctg tatatacgca taaccgatcg
    48781 tataaaggac cttatcatta ctgctggtgg taagaacatc gctccacagg ttatagaaac
    48841 catgttagga gaggatttgt acattgagca ggtggttgta gtgggtgacg gacggaaata
    48901 tataagtgcg ctcatagttc ctaattttga gatgttacat gaatatgcaa aatcaaaagg
    48961 gattcagtat tcatcacgcc aggaactaat atctcatccg ggaatacttg agttttataa
    49021 caataaaatt gaaaagctga ccgaaggttt aggacaggta gagaaagtaa agcgctttac
    49081 ccttatgcct gctgagttta ctcaggataa tggtgagata acaccaacca tgaagatacg
    49141 gcgtaaagta attcaacagc gatatagtga tgtcattaac aagatgtatg aagattaagc
    49201 gttaataggg aaaaaaataa tattgtatat aaaatattga ttgaaataat ataaccataa
    49261 cattgttatg ttatggttag tgtattgaat tacgattatt agcaaaaagg gagttattat
    49321 gaaaacacga attacacaac tttttggtat caagtatcca attattttac cagggatgag
    49381 ctggataagc acacccgagc ttgttgctgc ggtttgtaat gcaggtggca ccggttatct
    49441 ggcaacagga cctttaacag ctgagcagac acgagcagcc attcaaagaa tacgtgaatt
    49501 aaccaataag ccttttggtg caggatgtac actgatcatg ccaggagcaa gagaaaatgc
    49561 agaggttatg ctggaagaaa aggtcccgat tattaatgtt tcattgggta agtgtgattg
    49621 gattgctgaa agggctcata aatacggcgg taaggttatt gcaactgttg ttactgagaa
    49681 gcatgcattg gctgcagaga aacagggtgc tgatgcatta caggttacgg ggcatgaggc
    49741 ggcagcacat ggtggacagg ttactacctt tgttcttgta cctgcaattg tagataaagt
    49801 taaaattcct gttgttgctg taggcggaat tgctgatggc agaggaatgg cagctgcatt
    49861 ggcattagga gctgaaggta taggaatggg tacccgattg tcaatgacaa aagaaagccc
    49921 tctccataaa aaaacaattg aagcccagtt gaaagcaggg atagaagata ccatatattc
    49981 caacagattt gatggcatct attgtcgtgt gttaaagaca aaatcagcag aaaaagcaat
    50041 aaaaaaaggt atgagtctgc ctcatgctgc tatacaatcg tataaaatag caaagatgat
    50101 gaacctgccc tattttaaat tggtattggg agttatgttg cagggcccta aaatgatgat
    50161 tcagctggca cattttgcaa cagcatttga taaaattaaa gttgctactg aagatggtaa
    50221 tttagaatat ggtgtacagc tcataggaca gtgtcagggt ttgataaatg atgttccagc
    50281 agtaaaagat gttatcgagc ggacagttaa agaggcaaaa gccatatatc aaaaaaattt
    50341 aaagaaattt taaaaatttg tttataataa gggggcacgt tatcgccccc ttatttttaa
    50401 agttataaat attattattt taaggcagtt gttatgacta tcaacaaaca ggtacaggat
    50461 aaaataatga aagcaactgt tatagttaat ccatcttctc gatcaggatt aaaggtttat
    50521 caaacgctaa cccattatat ggataattat tattatcatg aagatcaaat ttttatttta
    50581 attccttgac aaatttacca tcaaaaaata agtttgaaca ccaaattctg aaagagatga
    50641 atttcttttt tccacagttt tttgattaat atatatagcg tgctacgctt ataatctata
    50701 taatttttat tagaaatttt ctattaacat ttaacatcga agtgtgagag gctacaatga
    50761 gtatctggca aaggacattt tcattaaaga aatctcagat tgagaaaaaa tggtatatta
    50821 ttgatgctga gggaaaagta ctggggcggc ttgcagcgga gattgcacat gttttacgag
    50881 gaaagcacaa accaacctat tctgcacatc tggatatggg tgataatatt gtcgtaatca
    50941 atgcagaaaa aattttatta acaggtaaaa aaccagatga taaagagtat ttcttccatt
    51001 caggatatcc aggtggtatg aaatttgtaa atattaaaaa gatgttgcag aaaaaaccgg
    51061 aatatgtttt atatcatgct gtaaaaggga tgatgcccaa aaataaattg tcacgcaata
    51121 tcatgggaaa tttaaaaatt tatgcaggga aggatcatcc tcatcaagca caaaagccgg
    51181 aaaatctgga attaaaattt taataagata aatctatagg tataaataaa tacgaggtac
    51241 gtactatggc aaacgcagtt aattatgcaa cggggagaag gaaatcagct gttgcacgag
    51301 tttggttgaa accagggaat ggggctattg ttatcaacag taagcctatt aatgaatatt
    51361 ttagacgaca gactcttgaa ttggttgtac gacagccgct tgaagaagcc ggggtacttg
    51421 cttcatttga tgtgtatgca acagtaaatg ggggaggatg gacaggacag gctggagctg
    51481 taaagttagg catagcacga tgtttgtcgg cgcttgatga aaagtataaa aaatcaatgc
    51541 gacaggcagg attcctaacc cgtgattcac gggaagtgga acggaagaaa tacggacaga
    51601 agaaagcaag gaaaagattc caattttcaa aacgataata atgagaatac aatttttctt
    51661 tacaaaaagg atgccgctga gcatcctttt ttattgttaa atattaatag tatggtgagc
    51721 tttcatggta ataatagata tagaatattc tgatcgctat atcatgataa cgttagatag
    51781 tggtgagcat ttaaaaattg attctgtttc agtaccacag ggattgctgc gaaagggtac
    51841 tgaaattgat attgatgcat atgaacatat taaagaagag tcaaagctgt ttgaatgtaa
    51901 ggaaaaagct ctttactata ttgggctgag agatagatcg gagcatgaaa taacaaaata
    51961 tcttataaaa aaaggatttg ataagaatac aatatataga gttataaaat tatttataga
    52021 aaaaggattt atagatgact atcactatgc attacagtat gtccgttcga cacatgaaca
    52081 caaagttgtt ggcaacaggg tgatagaaaa aaaattgttt gaacgaggca tccataaatc
    52141 gttaataaaa aaagcaataa aaaaaatgca ggataacgag cctgatattg agcgcatata
    52201 tcagatagca caaaaaaaat ataatcgtgt aaaaggaaaa gagcatgcca gggaaaaggt
    52261 tggattgttt ttgcagcaaa gaggttttag ctgggataca ataaaagagg ttttgaacag
    52321 gcttggtgat aataatagtg ataataataa ttttattgat gattaggcaa tccatgatac
    52381 aatcgttttg tggaaataaa taatattgaa attgtagcac caatttttta tattgttctc
    52441 atttgatatg ttgttatgtt attgattgag tagccgatgc agagggttaa tcacaattta
    52501 taataatatt gattttatga aattgattac aagtatacaa caatgagata caaaaacaac
    52561 catgcattgt tacagggaaa ggttcaacaa gataatcact acactatttt attattgaga
    52621 gagaggaatt cagcattatg atgaaagttc atgatttgcg agttgctttt attgaatact
    52681 ttaaacagcg agatcacagg atagttccat caagctcgct cattccgcag gatgatccta
    52741 cattgctctt taccacggct ggcatggttc agtttaaacc tatgtttgct ggtacaatta
    52801 atgcagaata cacacgtgct gcttctatac aaaaatgttt acgtaccagt gatttagcaa
    52861 acgttgggaa aacaaaacgg cactgcactt tttttgagat gctgggtaat ttttcatttg
    52921 gcgattattt taagaaagag gctatccagt gggcatggga attttccaca caggttattc
    52981 aatttccaga agagaatatc tgggtttcta tatataccga cgatgatgaa gcatttgata
    53041 tatggcatac catcattgga atacctgaac aaaagattgt tcgtcttggc aaagaggata
    53101 atttctgggg tcctgcaggt gattcagggg catgtggtcc ctgctctgag ctgtatctgg
    53161 atagaggggc aagctttggg tgcggtaagc ctgactgtaa accaggatgt gattgtgaac
    53221 gatttttaga attctggaac ctggtgttta atcagtattt tcaggatact gaagggaagc
    53281 ttcacccact tcctaaaact ggtatcgata caggtatggg gctggaacgg ctggcaacat
    53341 tagtgcaaaa tgtcgatagt atctatcaaa cagatgaact caatgaattg gtaaactatg
    53401 ttataaacaa acgcaatatt cgctatgagg gagacgcaca ggttgctgtc aatgttcttg
    53461 ttgaacatgc cagaacgctg acttttgcca tctcggatgg ggcatatccc tcaaatgaag
    53521 ggcgcgggta tgttttgcga cgtattctca ggagagcatt gcgctatagt cgtcagattg
    53581 gtatccatga gccatttgtt tataccatgg tggagcctat tgttacaata atgggaaaac
    53641 aatatccgga gctatcgtcc acaaaggaaa atgtgaaagc tgttattaaa ggtgaggagg
    53701 aacgcttttt agaaacaatt gaaaatggta tggacaggct ggatgaaata gttaatgccc
    53761 tgaaaaagaa gggagaaaaa acaatacagg ggaatgatgt ctttgtattg tacgatacct
    53821 ttggctttcc ggttgaaatg acagaagaga tagcacagga acatgggatg actattgata
    53881 aagttggttt tgaaaaaaag atggaagagc aacgtacacg agggcgccag agctggaaag
    53941 gtgcagaagg aagtattgat actattgttg gacagttagc aaagattatc cagagaacag
    54001 agtttgaagg atatgaaaaa tatgattcgg aatctgatgt tgttgccatg tcaaattttg
    54061 aagatttgaa ggtacagctt gcagatggtg agaggggtat aatagttgtc aagaatacac
    54121 cgttttatgg tgagtcgggt gggcaggtag gtgatactgg ttatatatat ggtgataatg
    54181 gaactttcag agtagaggat acaaaaaaaa gcggcgacct cattattcat gtgggacaga
    54241 tggagaaagg atatttacaa gaaggggagc atgtacgctt gtccattgat ggaatacgta
    54301 gaaattgtat acgggcaaat cacactgcaa cacatctgct gcaggcagct ttacgcaatg
    54361 ttttagggca gcatgttaaa caggcaggtt cattggttga accggagcgt tttagatttg
    54421 attttaccca ttttaaggca atgtcacatg atgaattatt gcaggttgaa actatggtga
    54481 atgaaaagat atgggcatgc ttgccggttg aaaccagtgt tatgccattg caggatgcaa
    54541 tacatgcggg agcaatggct gtgtttggtg aaaaatattc ggacattgtg cggatggtgt
    54601 cggttaataa tttcagcaag gagctttgcg gtggtacgca tgtagataat accggaaaaa
    54661 ttggtgtttt caggataatg catgaggctt caccgggtgc aggggtacgg aggatcgaag
    54721 gtgtgacgct taaaggagca tatgagcgtc tgttacatga tcagcacctg cttggcaggg
    54781 tgatgcgttt gcttgaggtt aatgaatcaa atgaatcaaa tgtagagaaa aaaataactg
    54841 agatggtaga gaaggtatcg ttattagaaa aagaactgga aaaaacaaaa gaaaagggac
    54901 ttaaaagtat agcaaaagat ttatatgaca aagcagaaac aattaatggc ataaaatttg
    54961 taagcgctgt attacaaaac atggatgctg aagcattacg ctcagtggct gattttgttc
    55021 gggagctatc cgttgcagtg gtggtgtgcc ttgcttcttc acatgatgag aaggctttta
    55081 ttattgctgc tgctaacaaa atagctgtag ataaaggtat tgattgcaat gctatcatta
    55141 aagctgttgc aaagaagatt ggtggtggcg gcggcggcag gaaagatctg gcacaggcag
    55201 gtggtaaatc ccctgaaaat gttgggattg ctttatccat gtgtaaagag atactattga
    55261 atagttttaa ataacatatt ttttaatttg tcattaattt ttgaaatacc atctggtcgt
    55321 gatatataaa tatgtatatt ataattaata tattcaaggg ggcattatgg gcgaactgaa
    55381 aacaatggtc cggtattttt atgatacatg tgataggtta ccggacaggc cagcacaaaa
    55441 gtttaataaa gagctgtatg atgataacaa cgggctattt acttatgctg aacttaaaga
    55501 acggattgag gatattgcat gtgcattaat gaaattaggt tttccttata aagggcgagc
    55561 tgctatttta gctccaacaa gctatatgtg gacacaggtt gatatcgcaa ttgcatgtgc
    55621 aggtggtgca agtattacaa tttttcctac gctttcaaat aatgaagtta aatatattat
    55681 gaatgattcc agatgtacta ttgtttttgt tggcgataaa acacttctta aacaggttaa
    55741 ggaactagaa aaagatttga aaagccttaa aacaataata gtgatggata tgtcatttaa
    55801 gggtgataaa aagaaggtat tcggtattaa agattgtatt gccatgggac gggaatggcg
    55861 aaataatcag gcaaattata aaaaatataa taaacgcaaa gatagtgtaa cattagatga
    55921 ttggtttact attctttata catcgggtac aacaggacag gggaagggtg ttattttaac
    55981 ccattggtgt ggttcatcac ggttggaggg aacaacggaa tattttcaaa ggtataattt
    56041 tgatgttaat gagaatgatg ttacactgtg ttatcttcca ctttcgcatg tttttgaccg
    56101 cggttcatgt caatggctgg ctatcagtaa agggtcgtta atatgttatg ccgacaagcc
    56161 agcaaccctt attgaggata tgcaaaagta caatccaaca tggattaatt gtgttccacg
    56221 gctgtatgaa aaaatatata taaccatgca gcagcagatg gctgagaacc ctttacaaaa
    56281 aaaattattt aattgggcat tgaatgtagg attacaggct ttgaaatatc gtgaggatga
    56341 aaatgggtgt tataatatgc atccgaattt tgatctaaag tccaggctgc cactatggct
    56401 taagattcaa tattccattg ctgataaaat ttttgcaaag gtgcgtggtt tgtttggtaa
    56461 aagattccat cattcatttt cggcaagtgc tgcaatttcg cctgatcttt tgcgatttta
    56521 ctatgcgctg ggattggcag tcatagaagg gtatggttca acagaaagtt tcaatgctgc
    56581 gatactcaat ccaataacag catgtaaacc agggtatatg ggaaaagagg caaatgggag
    56641 caaatgccgt gtttcaaaag acggtgaact ggaactttca ggtgctggta ttttcaaagg
    56701 ctatctcaat aaaccaaaag aaacgaaaga aacttttacc aaagatggtt ggtttaagac
    56761 aggggatatt gtagaattat caagcgatgg ttattttaaa ttcattgagc ggaaaaaagg
    56821 gataatctgt acctctattg ggaaaaatgt tgcacctgcc aagattgaga atttattttc
    56881 tacaagtaac tgggtagaac aaatatttct tgtaggtgat gaaaagccat ttattactgc
    56941 cttaattgta ccaaattttg ccaacttcat ggcgctgttt aagcgagaag gtattacata
    57001 tgatgagtcg aaactggtat atgaagatgt tggtggcatg cagctttgcg tacaggttgg
    57061 ggatgatttc attaacaact atcgcttaaa agaattgatt gataacgatg taaaagaagc
    57121 aaataaacat ctggaaaagt ttgaacagat taagaattat accattttga atcagcggtt
    57181 tactgaatct aatgggatgc taacaccaac tcaaaaaact aaaaagcggg tgatagttga
    57241 agtgtttaaa gatgaaatta ataaaatgta cgcataaaaa tattattatt tttattttaa
    57301 tagagcaggg ttgtatcctg ctctatttgt ttttatagga cagtgtttat actatgccat
    57361 aggctaccat tgctttggca acctttaaga agcctgcaat gtttgcaccg gtgagataat
    57421 cccctttttt gccataagct tctgaggcat ccagacaact tttgtgaatg cttttcatga
    57481 taatctgtag tcgattatca acctcttctc gtgtccatga taaccgtaag ctattttgag
    57541 acatctccaa tccagatgta gcaacaccgc cagcatttgc tgctttgcca ggtgcaaaga
    57601 ggatattgtg ttcacgatat accttgattg cgtccagatt tgaaggcata tttgctcctt
    57661 cagaaacgca gatacatcca tttttgataa gtgcttcggc atgcttgcca tctatttcat
    57721 tttctgttgc tgaagggaat gccacactaa ttttgatacc ctgctcttta ataacatccc
    57781 acactgattt accttcaaaa taatgagcat ttttatattt ttcggcatat tctttaacac
    57841 gaccgcggcg aacattcttc aattccatga gaaatgctaa tttatctgga gttatgcctt
    57901 cttcatcaat gattgttcca tttgaatcgc tgcatgaaat aacctttccg ccaagttgtg
    57961 taaccttttc tatggtatac tgtgctacat tgccggagcc actgacggct acagtcattc
    58021 ctttaatttc ttttccaaca gtttttaaca tctctgctgc aaaataggtt gcaccatatc
    58081 ctgttgcttc tggtctaagc aagcttccgc catattcaag ccctttcccg gttaacactc
    58141 cagtatgatc attccgtatt tttttataat agccaaacat aaatcctatt tcacgggctc
    58201 ctacaccaat atcacctgct ggtacatcag tgtcaggacc aatatgtcgg tatagttcgc
    58261 gcatgaaaga ctggcaaaaa cgcatgacat ccatatctga ttttcctttt gggtcaaaat
    58321 cagatccccc ttttgcgcca cccataggta gcgttgtaag ggagttttta aatatttgtt
    58381 caaatccaag gaattttata atccccaaat tgactgaagg gtggaatcgc aaaccacctt
    58441 tatatggtcc tatggcgttg ttaaactgaa cgcggaaacc tctattaaca tggacattac
    58501 cattatcatc aacccatggt actctaaata taattactct atctggttca acaattcgtt
    58561 catagatttt tgccttcaca aattcgggat gtttttgaac agttggttca agtgtttcca
    58621 gaacctcatg aactgcttga ataaattctt tttgctctga atcctgcctt ttaaccttgt
    58681 ctattacttc tgatattact gacattgtaa tgcctcctgt atgatattaa agataaccat
    58741 tgcttataga taatgatgaa tcatcaaaac atgccaaata tatggaaatg aacacactaa
    58801 acaattgata taattgtcaa taatattttt ttataacatg atgtttatta aaaaaaatgg
    58861 ttggtgacag tacagttgac atgaggctaa tagaaatata atcaagcgtt tttagctaaa
    58921 caatagtatg aaaatggtat tagtttaact cataaaacat agtactttct gggtggtgat
    58981 atcagccata agttctacat cgtgttcttt tagatcaaaa acttggataa cgtgattata
    59041 ttgagaatca ggtagaagtt tagtaaaaat atcggggaat tgctcaagaa agttttgatg
    59101 ataaagaacg ttgcttatac cataaaataa tgctacataa aaaatatcgg tttctaccag
    59161 atcctgaaaa aaatgagtcc caaatgaaag ttcaggcatt atattttcgt tgatcaacgc
    59221 aagttcaacc agtactttaa tgttatttat ctcagcgaat ctaacaggaa cacctaatga
    59281 aggtgtagtt gtgccccatc ttcccggccc aatgagcaat gctggcattt cttcacgatc
    59341 ttgtattgcc ttgtttaatt tgcctacagt gcgtgccaca tcatattttt gggaaattga
    59401 aagtgagtta tacagatacg gatctacata gattattcgc ttgataggct gaactatact
    59461 gccccccagg aagttacccc gggaggtaaa aaagatatga tcgccgggta tatcttcagg
    59521 taatttcact ttttttccca atccttttat ctgattgggc ctgcattgca atatgttgat
    59581 gtaatacccc tcttctttat taaaatttac ggtaaactca atatcgacag ggtattgata
    59641 tctattatgg atagtttgta agattgtttt aatgttactg gtaaaatcag tgtgcattaa
    59701 tagttcatca aaagtaacga cccaatattc tttttgttcg ccattcatat ttttaatagt
    59761 ttgttcagct tcataatccc ttattcctaa aaggtgcatt ggaagattga gatcttgaat
    59821 gttgaataga tcgtccatct gtatggtttc tacttgattg gttgttatgt taagaacatc
    59881 tacattgtgt tgtgaaaatc gccgtacgtc tttaaaatca taaaggggtt tggagcgtgg
    59941 atcgtcaaga gcaactatgc gaggataatc accttcaaca cggttgacag ctcgtgtacc
    60001 aagaccaata acaagacgga tcatccctga ttcaggtttc atctcttttt tccatacata
    60061 ggtattatgt gaaaatccta caccacttat atcaggaaag aaataattgc cacggtgagt
    60121 gccggatacc cgttgtacca gaggtgccat ctgttcgttc attgtatgaa gccctcgctg
    60181 gagccggtac acaagtgcat cttcattcat ggtgctggaa taaatttgac gtactgcatc
    60241 aataaaagct tgataccgtt gagcgggaga accctgatta acaagaaata tactttcata
    60301 ttttccagca aatgcattac caaaagaatc ttccagaagg cttgatgaac ggacaataat
    60361 gggagattgt ccaaaatatt caatcatctg ctgaaattgc tccttgatgt catcggtaaa
    60421 aatgccatgc aacatttgtt gagcaatttc ttttgctaca gtgaagtatc cttcttgagt
    60481 tttttgttcc atgaataact tccaccaccc gttttgaaca atataggtat agaaaacatc
    60541 tgaacctatg taaaatgagt catggggttc tacaatatca ttccattgca ttctgttatc
    60601 actatgtaaa atacggcggg caatgagcat acccgctgct tttccaccta tatgacctgt
    60661 cccaatcatt ctgtttttga ttgcaataag gtcctccagg gtaaagatat tggcggctaa
    60721 ggtaagtaaa cgctcctccc gtccaattat taccttgcat agctgttcaa tcatctgttg
    60781 ttttttggtg gggtcctcgt ttaattctag taacatttca gctttaagaa aaagcctatc
    60841 ccagaaatca aggttccttt gtgttttttc taagcttgtt tgctgtatat gggatatgaa
    60901 tgtggtgaca tcatcacttc gggtaattgc agtaaaatag tctgcatttt ttttgtgagg
    60961 gaagaacatg gttggggaat agcgattttg aaccttcagg ggatgtatgt agcaatcgcc
    61021 attgcatgtg tatacatcaa tgagtaattg tgtggtatcg cgaatacgag ctattgtttt
    61081 aaatgagtgg ctattgcgca ataaagaaaa atatgcaaca gcgtttaatt cataaagata
    61141 tgggcatgta atgacaaaga aattccctat catgagatcg gttgcccatg ctgattgaag
    61201 ttcagaaaga ctatcaaata tataatatgc attctcacct tccctgttaa taatggtata
    61261 aattcgtttt gaaaagcttt caaagccctc atgtgcattg agataatgta tttttattcc
    61321 cggctgttca aagaggaggg gaggaaactc tgcaaagcgg atataattca aatttctacc
    61381 ttctgaaagt gcctttgaaa caaaaggatc aacaaatgtt ttataatcag caatggtatc
    61441 aacctgccac actacattat cacccataat gaggttgttt acaattttgt ctaagcatgg
    61501 gagtccggtg cttgctttat ttatagtatc cattatagat ctcctgatag taaggatgat
    61561 tattatagta taatatatat gtcatttgta aactattcta tataatgtag ctgtagcagg
    61621 actgtaattg tgaaaatagt cagtggtaaa gtaaatagta tcgattattt agtatgatga
    61681 acctaattca tttaataaat tactaataaa atttaaattt ttttgggctg gctgatttct
    61741 ataggcttga aataaaccaa gcattctaat taaattgcat gaaattggta ttgaattatt
    61801 attagaaata ttatttacaa tcaattggaa tagtgttata taatcacaat tatcacgagc
    61861 agttctttgt tgagtgattc catggttgaa atcattaatt ttattaatac atgtttgtgg
    61921 atcaggaagt agcatagata taacctcccc gagttcaatg aaattattca aaaatcatgc
    61981 ccaacgttac cggcgtatgc gacgtttttg ccgtagcgat agcgaaggtt aaaatgtggc
    62041 gaagcccaag cgaacgacag taagcgcagc acataagctt tgttgtacgc agtggcaaaa
    62101 catcaagact tttttgcttt gagccatgac ggcgaaaagc aagtaataaa tttaatattt
    62161 tatctgacct caaaaatttt agaagctaag gttttattat ctccaactat caataattca
    62221 aatttacctt ttctattacc gacaaaagct ccagctgtaa tacttttttt attaaacccc
    62281 cacatagggg ctcttgaatc aattggatat gaaattatac ggaatggttg ttcaacacca
    62341 gcatctttta caacacttat tcgtaattct atttttgtaa caccaataaa cttataatga
    62401 ttatctagta tcattataaa ataatcatcg ctattaaaaa tatctgtttt tttagttatg
    62461 tgccaaccaa caatgtcttt gcctaaataa atatctgctc ctttatcata tgaccataca
    62521 tattttccag atttgttgat ataaccatat ttttcaccac ctaccagtgc taaaccattt
    62581 ttaaattctt ctgcataata aaactgtgta tctattacaa ttttaccaga tttatcaata
    62641 tatccatatt tatcagataa tttgactaga gcaagccctt cttcaccaaa atcataagca
    62701 tcttcaaatt gaggattgat tacaatttta ccagatttat caatatatcc atatttatca
    62761 gatagtttga ctatagcaag cccttcttca ccaaaactat aagcatcttc aaattgagga
    62821 ttgattacga ttttaccaga tttatcaata tatccatatt tatcagatag tttgactata
    62881 gcaagccctt cttcaccaaa actataagca tcttcaaatt gaggattgat tacgatttta
    62941 ccagatttat caatataccc atatttatca gatagtttga ctaaagcaag accttcctta
    63001 aaactataag catcttcaaa ttgaggattg attacaattt tacccgattt atcaatatat
    63061 ccccattttt ggtttatttt aactgctgca agcccttctt taccaaaact ataagcatct
    63121 tcaaattgag gattgattac aattttaccc gatttatcaa tatatcccca tttttggttt
    63181 attttaactg ctgcaagccc ttccttgcta aaatcataag ctttttcaaa ttgaggattg
    63241 attacaattt taccagattt atcaatatat ccatattttt tattcagcat aactacagca
    63301 agcccttctt caccaaaact ataagctttt tcaaattgag gattgattac gattttacca
    63361 gatttatcaa tatacccata tttatcagat agtttgacta aagcaagacc ttccttgcta
    63421 aaatcataag cttcttcaaa ttgaggattg attacaattt taccagattt atcaatatac
    63481 ccatatttac caatatcaat aagaaataaa tcattactac aaccggtaag atttacgaca
    63541 accaaaataa ttaaaagaaa aaaatttacg tgtttcatgg tattctcctc cggcaggtta
    63601 aattttattc cccacaaggc gggcacggac gcccggccaa agttatgttg ccattgcata
    63661 taatgttacc agtataatgc atagtagcat ggaacacgtt aaatttattt aatagattga
    63721 tctttatgtc aataaatatt ttatgttttg aatttagggc aattacgcat atgctgtgtt
    63781 atacgccgtt tttacaattg tttcactatc cagtgatttt tatggttttc ctttaaatta
    63841 tttaataata ttttggcttt ttctttatca acttctagtc tatattcata agcttttgca
    63901 ggtatgaaat ttggatctgc ttttcttaac tctgcaataa ttttctttgc atcttgctct
    63961 tgcttaagat taattaatgt ttcagcttta tataataata aaatgggatg aattttatta
    64021 aatctagata tgtattcttt tgttaaaaga tctaaacact ttaatgactg ttcaggaaaa
    64081 tattgattat ataattggta aaacaaaaat aaatatgcta agtctcgatt ttctatattt
    64141 atgtttttat taaattcgta caatttgtta attgattctt tgaatttctg atttatatat
    64201 ggtccttcat cagattgaaa actttcatat actaaattaa aaatagatgt aatattatca
    64261 ttttttgatt tattttttga attagcatag acaacccaaa atggaacaat tcccttatct
    64321 gtttttgtgt ctagtatacc atctccatca atatcaaata tataataggc atttggactt
    64381 gtaccctgta aaacaccaat gggatattta tctaatgcaa aaactattat tggtgatttg
    64441 ccatcaactt ttgtgtcata agctaatagt gttacaacac cttctggcca gtttgataat
    64501 ttttgatttg tcagtttata tttaaccgga ttagttttaa tataatttgt tacattatca
    64561 ccaattttta ataaatcata agagtaaata ttggtatgaa taaaaacagt aataattaaa
    64621 ggtaatagaa tttttttcat gtggtttcct ccttttagtt aaaaattgta aaaatgtcgt
    64681 ataacgtctc agcatagctg aagtttttgc cggagcggcc gtcttcatat cataggcgcg
    64741 gaggccgagc accgcagcgg ctttgcttag ttatatgaag tcgtgtgccc tgaagctgaa
    64801 tcagggttgc ctatatagag tgaagcagtt agtaggaaga atgtcaagaa gattcagcgc
    64861 aatctttatg cataatggca gagtcaaggg aacgatgaac tgccagagag ggttttccgg
    64921 aaacagtctc taaacccccc ctttggtgct gtcgggggta gtcccggtgg tactgaacca
    64981 aaggggcaaa acgatccatg caaatagacc cggtgaatct aaaggagata aatgaaagtg
    65041 aaccgccgat gagtcatcga gaataacttt ccccgatacc gaaatccgag ggactgctcc
    65101 ccgaggagaa caatgtccag cagtaaaccc gtattaatgg ctgtatggta tccggtgttc
    65161 ggcgcttcct gggggcttgt ggtggaaaat gtgatcagct cgctgcccgg ccgggaggct
    65221 gtattctatc ggacatcatc cggaaccgag atagacatgg tgctgggcag ggggagaaga
    65281 ctggtcgcgg tgtagtgcaa agcgtcggca tcaccagaaa caggaaaagg actggcaatc
    65341 gccatgaacg atcttgaaat acgggaagcg tggatcatcg ctcccgtgaa ggaaacctac
    65401 atgctcacga aaacggtccg cgtcgcggcg ctgtatgaat ttgtttcagc gctcgcggag
    65461 ggaagcagca aaagctaacg ggtgcaagcg gaatgtgatt ttgtagaacg atatgcggat
    65521 gaccagcaaa atttcgtcca acaaaaaggc ggtttcccgg ctgccttttc catgtggtaa
    65581 acggctattt tttatatctt ctgatataaa tatttgatta atttagttgt tgacaaaata
    65641 gcaattctat gaaataatat atgaatgaaa ttcggtttaa ttagaaatat ttgccaaatt
    65701 gccattttat ctctccttcc atttggcttt tcactgcatg ccaatgaaaa tattcagcag
    65761 aggagtactt atatatcaga ctgggaatct gattccagat acatatggtc gctgttaaat
    65821 ggagtcgaaa agtatattta tgatgaatct gatacggagg tacaaaggga atacaaggaa
    65881 aagcagtatg cgaaagaact gtatagaata aacgctgcgt ataggggtaa acagttctgc
    65941 atgcgatctg taaaattgat tgatgtaagg caagaagagt ctaagacgct gaatgaattt
    66001 gggcggcaga aaatcattaa ttttctcatt tccaagatgg ggaaaaataa attttcgcag
    66061 atggttgagt ccgctaagaa gaataaccag tttgataatg aacagtggtt ttctttcgtg
    66121 ctcagcaatc tcggttcgtc aatagcgttc gtactggaat taaatatgtg cgagcgttgt
    66181 tatgacaaat caatactgta taatgcttat tacgaggtac cggtgcctga tcagagcgag
    66241 gaaaaagagg ccggcactaa cggatattca gatgccaccg gcattcttga tattaaaggt
    66301 ataagcaatg aagatggaaa taatccaagt aatcgtaata tagtgcctgt tgatataatt
    66361 aagagctttt caaaagaaga ggatgtgata aaattgaaaa aaggcgaggt ttcacctctt
    66421 cagggagttt tatcaagaat cgagtatgaa tatgaatatg gtatcaggaa aattaccata
    66481 aggttaaaat aagtttaaaa tcattttttt aggagggtta gctaatggct acagcaggat
    66541 ttattgtggg aattctttcg ttgtttggta caatgcttgg atgtattccc cttttagggg
    66601 cgttgaattg ggtgaatatt ccttttggtt ttttaggtat cattctttct ggcattgcca
    66661 tggcaagcag taataagcaa acaaagggga aagctaccgc aggtcttgta atgtcaataa
    66721 ttgcagtaat ttttggagca atacgattga tgatgggtgg gggaattgta tagaattaag
    66781 tctatatatt gattattatt ccataataat ttggctatag gggttgataa aatttattta
    66841 atcaggctat cgaattttta acttttaaac tattgacttc cccttactgc tatataacaa
    66901 atggactttc taccagatag catcgcttaa aaaacaagcc tcatcgcttc acgataccct
    66961 tcctttttat ctcgggtgat tgtccgaatt cattctgaaa aaaaagtggt ataccatggg
    67021 gacattgata ccagtttgtt tcaggacaat aggtgaaatg acatgtatca aacgctcctt
    67081 tcttctgaac accacgcctg tcaacatcat acc
//
LOCUS       DAOOJH010000004        62276 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_546_length_62276_cov_9.757413, whole genome shotgun sequence.
ACCESSION   DAOOJH010000004 DAOOJH010000000
VERSION     DAOOJH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 62276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62276
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_546_length_62276_cov_9.757413"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..567)
                     /locus_tag="PLJ75_00995"
     CDS             complement(163..567)
                     /locus_tag="PLJ75_00995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HPD03828.1"
                     /translation="MKIVLDTNVILSAFITQGLSSRVIDICIDKHQLFISQWIVNEVI
                     EKLNTKFKTPKKELKRVKDFLLTAFRFIQPEGEMPDLCKDKDDNNILYLAQYIDADLI
                     ITGDKDLLELKIIKKSRIINPRTFMEKYHNMH"
     gene            complement(564..782)
                     /locus_tag="PLJ75_01000"
     CDS             complement(564..782)
                     /locus_tag="PLJ75_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HPD03829.1"
                     /translation="MREILTISIDSKLKEKVEKAAEKFNVSKSELVKKAIEKYILHEE
                     FQEIRSMLIPFGEKAGYFTDEDIFKDFS"
     gene            complement(1022..2152)
                     /locus_tag="PLJ75_01005"
     CDS             complement(1022..2152)
                     /locus_tag="PLJ75_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD03830.1"
                     /translation="MAESILHFFMFYGAAFSVIIAIGQLLQKKSETINRIYAFSFLGL
                     GIWLLQISIFSSGLFDGTAVGYYVCIFSIPIVFTVPPLMVIRYTWIMSSLFNFQKKYY
                     LLFSPVVVSLLYIMIQFFIDSDCGYTCFPALPILSHTFNELSLYDTILYLFFIFPNIY
                     LISFMSQNLFMTSYIWKKSNWNKVPGVSRWGYIFAMSIVISNIVYVIGVFISLRVVEW
                     AVILANTAMTFVYLVTQRLPDYNKLLKNEALKARYERSRIKGLDVDSVIQRLYEIMVD
                     EKAFADEELSLPDLARDLGITVHQLSEILNEHIKKNFNTFVNEFRVKEAQKLLVDEPD
                     RSIISIGFAVGFNSNTTFCTVFSKMTGVSPNQFRKDNIKKDE"
     gene            2319..4160
                     /locus_tag="PLJ75_01010"
     CDS             2319..4160
                     /locus_tag="PLJ75_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD03831.1"
                     /translation="MAQHENVNYEKEFEEYLKRQKHLGLMALDRAEKFGDSKIAVRHK
                     AYGTWEAFTWKQFGDLIKAAAKGLLSLGVKEFEFVGIFSNNRVEWAVVDYACFTIRAC
                     SVPVYATNSAKELEYIVNDCGIRILFVEKQEHYDKAMSVFDNCKTLEKIIVFDRNTNI
                     VNSDKVMLFDDFIQLGKNSGLDAQLQERLSKVQSDDLSTLIYTSGTTGEPKGVMLTHK
                     NWFAMLFGTGYHITVEPTDVNLAFLPLSHVFERAWSYFILCNNAQVDYCHDTKALLDF
                     LKESRPHFMCSVPRMWEKIHATIMDGIKNSPPAKQKIFNWALEVGGNYQYLKKDNKSI
                     PLGLRLKHAIATKLVLHKIRDIFGGRNKVFNCGGSAFSGEISEFFFKAGVFILQGYGL
                     TECFVICVSNPHRNKFGTCGPVVPLMQVRISDEGEIQAKGPSMTQGYWNKPELTKALF
                     TPDGWVKTGDVGFIDSDGFITVTDRLKDLFKTSGGKYIAPQQIETLLNEDFYFEQVAA
                     IGDGRKFVSALIVPAFEPLKNWAKSNNIPFTSHEDLIKHPAVIQFYRERIDELTKNLG
                     QVEKIKKFTLLPNEFTQEKGEITPTQKIRRKVINEHYKDIIEQMYKD"
     gene            4312..5184
                     /locus_tag="PLJ75_01015"
     CDS             4312..5184
                     /locus_tag="PLJ75_01015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD03832.1"
                     /translation="MHINIYTLDEITKTYENIDDHLSTRQIIRKFSQNKNDIRLLSLE
                     LCNLSHYNNILDLGCGYGFFTEALNNRIASGACITGVDVIGGENKRMFLSTCVQSGYN
                     AQFIEDSAHIICNIPDKTYDCIIASYSLYFFPDLINHIARILTNDGTFIALTHSELTL
                     SQITSIFIESIHHVIPLSDSSLHIAKLFKSFSAENGFVQLQPYFDYIMYLRYPNNLIF
                     KSSDIDHCISYIHKKQYLFFKDIALLDRDEYDALINDFYNRLRWHVSSYKDFIVNKDD
                     AIFIARNPVKHINE"
     gene            5292..6293
                     /locus_tag="PLJ75_01020"
     CDS             5292..6293
                     /locus_tag="PLJ75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD03833.1"
                     /translation="MKKKRIYCSFCGAPITARFIDEKYRDHCDNCNTTFYENPLPVAS
                     CIVINKNREVLLVLRKNEPYKDMWCLPIGFAESGESIEQAALRELQEEAGVNGEIVRI
                     IDVDTVSNYFYGDLAIITFEVQQLSETYKPGDDAADAKFFSLDNYPQLAWESNEKALH
                     KFIDLYKDIWTMIDSLKTIQPDITTHHDIPREKSKQYTFIANIIASMIDDEIELFTQQ
                     WKREMPNVNNNIFHFLIFIHQMALHTIKLWLTGNRIWKNFREFYIMGKQLKKENISLK
                     DILSAIALSRKCIWIQAIEKNILHSPLEVYTMLEINNRIILFYDKISYFLIKGYEHS"
     gene            complement(6341..8419)
                     /gene="fusA"
                     /locus_tag="PLJ75_01025"
     CDS             complement(6341..8419)
                     /gene="fusA"
                     /locus_tag="PLJ75_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD03834.1"
                     /translation="MPLRNIRNIGIAAHIDAGKTTVTERILYFTGVTRKLGEVHDGQA
                     VMDFMKQEQERGITISSASISCQWNDHNINIIDTPGHVDFTVEVERSLRVIDGLIAVF
                     CAVGGVEPQSETVWNQADRYKVPRIAFINKMDRVGADFYEVVSQMDRYLDANPVPFQI
                     PIGVEDSFEGCIDLVEQRAIVFRENERLIVDIPDDYKGKAKEARKHIIEKLADFDDEI
                     MELYLAEKEMPSELIKKTAREATLKMLITPVFCGAAYKNKGIQLLLDAVVDYLPSPVD
                     SGAIVGIDVDYPEKTHTRHPSTHEPFAALAFKLINDLYVGQQTFIRIYSGKLQSGMQV
                     YNSTKGKYERIGRIMRIHAKEREEISEAGAGEIVALIGLKFTKTGDTLCDTNNPLLLE
                     SIYVPPSVIDLQVMPASKKEEEKLGIALHKLATEDPSFNVRVDEETGETIISGMGELH
                     LEIMVDRLKEEFGLNVQVGEPSVAFRETITAEAEQETKFVKQTGGKGQYAHCVMRIEP
                     NPGKGYEFIDHIKGGAIPNEFIPSIQKGIHAAMEEGILADFPVVDVRVVLLDGSFHPV
                     DSSDMAFRTCASICFKEAFMKAKPILLEPQMKIEVNTPDEYIGDVIGDLNRRRGKVES
                     MRRFRKGAQKVNGFVPLMEMFGYATTLRSLSSGRANYAMEFDKYVALPKEMQEKVLAK
                     IKEKKRRQSA"
     gene            8539..8706
                     /locus_tag="PLJ75_01030"
     CDS             8539..8706
                     /locus_tag="PLJ75_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03835.1"
                     /translation="MVGHGYKDSKHVNILKIFFIFFNKTYHFYKITNLYGILTTILVA
                     IVTLNEPFLMQ"
     gene            8976..10427
                     /locus_tag="PLJ75_01035"
     CDS             8976..10427
                     /locus_tag="PLJ75_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012797814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03836.1"
                     /translation="MDNNDRSLSIFGNPIPKHTYNKAMKQKARFAKKFGFNPNDRYEL
                     SALPNPILKKYCNLYTVSQEEGSEIAKSKSIIIGTIRMGYGHYRIAMAIASAAHSMGI
                     VPYWFDLLSFDTAGAKIIKHMDNLYSFGSRLSQQFYLFNKLYWEHLTAIGFKKLTYNA
                     TDQAMTQLFANIYQNLPQSVPFVATHAWASQAAVHAGTKKVINMIPDNWPLALHLSEG
                     AIHTVQTPSAYFGYRTLKNMGKKNQILNPMPKDDIYYTGHYVDHELVANVEKDCNARL
                     TRLKNKKPRRFLLSIGGAGAQQKLCMDIIMHCIPLLENEKLTLIINTGDHKAIFDMIL
                     NTGFSPRVPKTSYTQWAETEKLVNTLTTRDVPGLHIVYNEDIFSAVYATNILMRVCDC
                     LITKPSELAFYPVPKLFVAHVGGHEMWGAIRGAEIGDSTVECETVQHTLQAMDLLIYD
                     DDLLTLYCNNIIKAKSIGIYNGAYEVIKLAFGK"
     gene            complement(10492..11286)
                     /locus_tag="PLJ75_01040"
     CDS             complement(10492..11286)
                     /locus_tag="PLJ75_01040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPD03837.1"
                     /translation="MGNRSDKPMNPLELLAYVLAKEIKDHEVVYIGTGLPMVAGILAR
                     KTHAPNIVLVYESGAQDPMEGGMPWSVGGPATWRKSPLILEMSYSFGQIYNGYVDKAF
                     LGFAQIDMYGNVNTHMIGKDFLKPDHRLTGSGGNNDLSSLAENMILVGLQSPQKFVPR
                     VDFITSPGFIDGGDSRQKAGLLGKGPVTVITQAAVFDFEPETKRMRVKSLHPGVSFDL
                     CQLACGFELVKPAGDIPVTEVPSKEILEIIRNDVDPRGVFTSIPGM"
     gene            complement(11335..12444)
                     /locus_tag="PLJ75_01045"
     CDS             complement(11335..12444)
                     /locus_tag="PLJ75_01045"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPD03838.1"
                     /translation="MGKFSFNEEAVKNLKITDTSVLERLIDFEHARKWHLSKDRSRKD
                     KRMSLKEAVAEYVHDNDIMTDGGFCYVRTSIQSYFEVIRQQKKNLQFCSSPNTNHSYM
                     INAGCARYSHNSYLGAEMRGIDRNYTRQLKAGKVTILSEWSHGTMAQGFKAAQLGLPG
                     VFSKQLLGSDMIKYNPYVKVMQNPMRSDPDPVAYIPAIYPDVSIIHVHAADKKGNARF
                     FGPAINDIAMAAATRKLIITAEEIVPESDIRNNNKGVSIPAMYVDAVVELPYGAVPGQ
                     MPGCYYWSRQWWEMAFRFGMKSDENFQKFLDYWVFGVKDQYEFIEKLGGSKWIAESRR
                     LTKAEEYDNEDEGVDFNYEEFQGDWPEDITKMKFY"
     gene            complement(12786..13598)
                     /locus_tag="PLJ75_01050"
     CDS             complement(12786..13598)
                     /locus_tag="PLJ75_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPD03839.1"
                     /translation="MKTDRGYQKSFLIDIDGVIVRGNSLISGAKDFIGNLQKGNFPFL
                     FLTNNSYYTPKELSERFFTLGLDVDESRFYTSAMATASFLVYQKPGCSAYVIGGRGLI
                     QELEKNGIVISYKNPDYVIIGETEEYDYSRIIEATYLIQEGAKFIATNPDLTGPTDRG
                     PVPACGALVAPVEKVTAVTPYFLGKPNPAMMYWARKKLNVHSANCFMIGDRMDTDILG
                     GSEAGMTCVLVLSGVTTLAMVDRFPYQPDYIVEHVGEIDPDTILSRRERIKE"
     gene            complement(13607..14446)
                     /locus_tag="PLJ75_01055"
     CDS             complement(13607..14446)
                     /locus_tag="PLJ75_01055"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD03840.1"
                     /translation="MELIDLHCHTTASDGIYKPCAIIDYAIEHGVKILAITDHDTIDG
                     LDEALCYARQKSFTLVPGIEFSIEYPHGSFHLVGMGIDHSNEELKRELSHLQKLRKDR
                     IYKIINDLQVHGIDISDKEVEQEAVGNSPGRPHVARVLVKKGYASDVKDVFVNFMVKG
                     KPGYVKKDKITFYKAIQLIKDSGGIPIVAHPISLGFGSWAEGESLIKDLFDGGIQGIE
                     VYSAMHSLEDVEQFLAIAKKYSLLISGGSDFHGDKQEVMGYYADGKPIPFEIGQQFLK
                     VLR"
     gene            complement(14529..15293)
                     /locus_tag="PLJ75_01060"
     CDS             complement(14529..15293)
                     /locus_tag="PLJ75_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPD03841.1"
                     /translation="MITENKQYYIGCSGWNYKHWKGLFYPETVPQYRWLQYYASIFTT
                     VEINATFYRQFKDATYTSWYNRVPNEFCYSLKLSRYITHIKRLLHVDDAIQRCVTSAS
                     LLRDKLGVIVIQLPPSLKFDEQLVGDFLLLLPHNYRYAIEARHSSWLCDSCFSLLQQH
                     NIAWCIADTASRYPYHEVVTANFVYIRLHGSKKLYASEYTMRELEEWANKIIAWNKNT
                     YCYFDNDVNAYAVFNALSLKRLLHITSPLPCSEFTV"
     gene            16068..17744
                     /locus_tag="PLJ75_01065"
     CDS             16068..17744
                     /locus_tag="PLJ75_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPD03842.1"
                     /translation="MEIPPATNFIEEIINEDLKNNVCTGVITRFPPEPNGYLHIGHAK
                     SICLNFGIAIKYNGRCNLRLDDTNPEKEDVEFVESIKHDIQWLGFNWYGEVRHASDYF
                     QQLYLWAIVLIKNNKAYVCDMSAEEIAATRGTPSQPGKESPYRNRSVEENLDLFERMK
                     NGEFPDGSKTLRAKIDMSSPNMHMRDPVMYRIKHATHHRTGDTWCIYPTYDWAHGQSD
                     YIEGITHSICTLEFEVHRPLYDWFLDQIASGQRPRQIEFARLNLSYTVMSKRMLLKLV
                     QQGYVSGWDDPRMPTIAGLRRRGYTPDSIKIFARTVGVAKKDSMVDIALLEHCIREDL
                     NTKANRVMCVLNPLKVIITNYPENQVEYVEGINNPENQVAGTRKIPFSRIIYIERDDF
                     MENPPKKYFRLSPGSEVRLRYTYFIQCTDVIKDASGNIIELHCTYDPQTKDGYAPDGR
                     KVKGTIHWVSAHHSLPVEVRLYDRLFTREDPSQVDDGSDFTDYINPDSLKTVHGFIEP
                     SVANAKAFTNFQFERLGYFTVDPDSHDNNVIFSRTVTLKDTWAKIVQKEG"
     gene            17816..17888
                     /locus_tag="PLJ75_01070"
     tRNA            17816..17888
                     /locus_tag="PLJ75_01070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17849..17851,aa:Gln,seq:ctg)
     gene            17957..19210
                     /locus_tag="PLJ75_01075"
     CDS             17957..19210
                     /locus_tag="PLJ75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPD03843.1"
                     /translation="MYTVALIIFIITYSGIMVTRLPGINIDRPSAAYFGAIAMIAAGV
                     LSFEEAIAAIDFNTIALLLGMMIVIATLELDGFFAFVAHKTIAFSKTPSQLLWIITFT
                     TGIASAFLVNDAVVLMFTPVIISICRHAQLDPKPYLIAEILASNIGSSMTITGNPQNM
                     LIGIYSKIPYIYFILHLFPIAILGMIIIIIVIKRIYSQTFEAPSLLHYDENEENEYDY
                     NLSSMIFSVPIFLIMTILFLFHHMLHLSIPLIALIGSSFILLLGKIKPSKIIKEIDWV
                     LLLFFASLFIVVKGAEKAGILNIVMHYSQLNNTFYSTIVLHGISLISSQIISNVPFVI
                     LLVPILKQNNSKLLWLALASSSTLAGNATIIGAMANLIVLESAARAGIKISFIEFLKP
                     GLMVTIITLLMSIVLLYLQLNFQLL"
     gene            19535..19876
                     /locus_tag="PLJ75_01080"
     CDS             19535..19876
                     /locus_tag="PLJ75_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD03844.1"
                     /translation="MQIDVHKEGPLLILTLNGRYDISKIHNFETIYRQNLEDNPKIIA
                     LNLRDLKYIDSSGIGSLVRCMNLALKNNIEFICYDINENVESIFKIAKLDQFISILTH
                     DEFIKKYIKKQ"
     gene            complement(19863..20855)
                     /locus_tag="PLJ75_01085"
     CDS             complement(19863..20855)
                     /locus_tag="PLJ75_01085"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPD03845.1"
                     /translation="MAIASFLQYTTMKMKKVTVLLYCILLSAIVTITVLFLLFNHFIL
                     NQYHLSLLLKSVFFIAVIIVIFNISFIIIAKVLHKQYYGVARSMLKVLKQYEKGNYTY
                     LFSIDNNGFDSILKYMQKLLAGSNNPEVSLDKQLDEKVFAIISVLISAMEDKDPYTSG
                     HTARVAQYSLAIGKHLGLPKEKMRLLNHAAAAHDIGKIGIPIEIIEKNGKLTDREKLL
                     LQSHPIRGMKILSTLDYLKDIATIVMYHHERIDGQGYYELSGNKIPVEAKIIAVADAL
                     DAMLSDRPYRKGLSLHEAKQELEKNKGTQFDPLIVDALIQLLEEKGTLKELIAF"
     gene            21043..22017
                     /gene="trpS"
                     /locus_tag="PLJ75_01090"
     CDS             21043..22017
                     /gene="trpS"
                     /locus_tag="PLJ75_01090"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPD03846.1"
                     /translation="MSRILSGIQPSGTLHIGNYFAMMKPMIELQYNNELFCFIVNYHA
                     LTSIHDPRLLAQNTVDAAMDFIALGLDPDKAHFWVQADIPEVTELSWILSCHTSMGLL
                     ERSHSYKDKIAKGIIPNAGLFTYPVLMAADILLFNAELVPVGKDQKQHLEITRDIAQH
                     FNQMYGEIFVIPEAMIPEDIAVIPGIDGQKMSKSYNNTINIFEDEASLKKKVMRIVTD
                     SQPVEAPKNPDSCNLFALYRLFAEPEKTQILKDRYLKGGIGYGEVKKELFAMIWEYFA
                     PFRAKRKALENNKHDIASILKKGAEKTRPLAIETIQRVKEATGLKYLK"
     gene            complement(22041..23444)
                     /locus_tag="PLJ75_01095"
     CDS             complement(22041..23444)
                     /locus_tag="PLJ75_01095"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPD03847.1"
                     /translation="MKMKFHGITVCVIILCAIVTVDAKNTIKKTQQGTSIADLNTFNE
                     QLVTITNKAIPTVVSISAEKKMGSPRDARKMPMPFPMPKEAKAVGSGVIVDKRGYIVT
                     NNHVVKNTKSITVTLFDKRTFPCELIGSDPATDIAVIKIQGDVPKDIPVAEMADSDTL
                     KVGEIAIAIGNPFGFGHTVTMGIISATGREGFGLADYEYFIQTDAAINPGNSGGALIN
                     VTGKLVGINTAIFSRNGGYMGIGFAIPSNMVKQVVDELIQKGKVTRGWLGVYIQNITP
                     EIAKNFKYDGKQGVVVADVMNNSPAKNANLEIGDIIVSVNGTTVSDINQLRRNVAALK
                     PGSTAKVKIFRAGKEMDVDVIIAELPQQQAMEEKAVELDIIGLRVGDITEENAYKFRI
                     TDTTGVIVMEVSEDGPAYQAGMMVGDIIKEIENTPVENVSRYNELLAHYQKKETLLLL
                     VKRGGIHKFVVIKKSEK"
     gene            24047..25069
                     /locus_tag="PLJ75_01100"
     CDS             24047..25069
                     /locus_tag="PLJ75_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPD03848.1"
                     /translation="MKKIYSPMVIVAMFGAQLLLSCSHPQRYSLSQMAMGTIVTITVD
                     APSHEKAHNAIQAAFDKIKQIEALMSLYKQDSDVSKINANSKTHVSKDTFTVIEQSLE
                     LSHKTNGLFDITFASIGKLWNIQSPNFIPPSPQTINTYKPCIGYQRIHLHPENLEVIK
                     EKPCIQIGLGGIAKGYAIDHAIVTLKNMGIKEAIVEAGGDLKVIGNNYGHGYTIAIKH
                     PRKEGICGTVLLFNGQSITTSGDYERFVIYKGKRYHHILNPKTGYPATSDVISVSVIA
                     SNATVCDGYATALFIMGSEQAMSFLLHNASLQLQCIIIDSTMKIYVSKSLVPCTSFKG
                     YTVVPF"
     gene            25214..26101
                     /locus_tag="PLJ75_01105"
     CDS             25214..26101
                     /locus_tag="PLJ75_01105"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD03849.1"
                     /translation="MELSEYAGQSTRINDFYIHVSDGIQLYCIDFIPKHDNVSKPIIF
                     FVAGWLSHISGWKDVLRSLTDEYHVIYIETREKISSKIPDGKSCTDFSFNVMRMVLDL
                     HEVIQQIIPKKRTFVMAGSSLGSSVILEYLAMRTREPECALLIGPIPVFRFPPILGDI
                     VPNLPPSWYNVLKKLVIWYLKTFRLNTRKEKEQLIKYTNTINAADPYKLKPNALSLKN
                     YSLWDKLAFIETPCYIFGAKTDTLHATEDIKRLLQELNRADYIECESNKETHSAIVAL
                     YFINILKNKLYKKIKAKKP"
     gene            26167..26655
                     /locus_tag="PLJ75_01110"
     CDS             26167..26655
                     /locus_tag="PLJ75_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPD03850.1"
                     /translation="MSLCKVDIVTIVLDEESNTPIVVLQDNETGNILPIMIAPLEASL
                     IAIELEGKKPIRPLTHDLIIAMLHTFHYSLESVVIDDLKDNIYFAKLNLVAPDSSEMI
                     IDCRPSDAMAIALRAKAPIYVKKKVFAIAQGLDNLSQIDKETMKEVLEDIDIDDVGGK
                     IM"
     gene            26679..28526
                     /locus_tag="PLJ75_01115"
     CDS             26679..28526
                     /locus_tag="PLJ75_01115"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HPD03851.1"
                     /translation="MYTEKNISALHNIIRDLTEIDSRLNEYIDTTMWISDPHGAGDRF
                     VSILKGRFGLVWRICYEALPKTFSKEKIYYLGRIIRKERYFEDDVYRLDRQDIISSLI
                     RIIQYRVQNVRDFDEIRININKDLKHVLENLILNYPVPDIIYENELIADKIISSLCKI
                     VKQVILGHLIVLGDVFDRGDEPDKIIRILNQKDIKRYLTFIWGNHDILWMGASAGNQS
                     LIAEALRITTRYDNLAFLERIGINIDKLKNFALSTYTGEITGNFKAKQDVSRRMEKAL
                     SIIQFKLEEQTIHNNPEFNMESRLWLNKLAEMLKNNNTDGLTDTHFPTIDLNDPTKLS
                     PEEEDIINDLSYQFAISKKIRYLMEFLFEHGKLYHIHNYILNIHALIPSTEDGDFEEF
                     SGYKGKELLDYLQHRIKTIGKNYLEGKLQDPGDLALMFYLWCGSKSPFFGKNAMKTFE
                     RYYLVDKTTHKEKLLYWGENLLKNEFMDKIMHEFGVERIVYGHTPIDIRKGEKIASPD
                     GRAINIDGGFSDAYLNRGHALIQTPYSLYAIILPTPEEIINARQKKEPVNLQVETIDN
                     FETPLQVKDTYTGKLLIKQRNELYNKLLEYTDHYLLATDNDKKAFYSLV"
     gene            complement(28593..28991)
                     /gene="atpC"
                     /locus_tag="PLJ75_01120"
     CDS             complement(28593..28991)
                     /gene="atpC"
                     /locus_tag="PLJ75_01120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPD03852.1"
                     /translation="MALKLNCNVLTPERQIYEGQIDFAVVQAYDGEMGFLYNHAPLIS
                     ELGTGEIRLRNGDATEYFYVDGGFVEIKNNSLIILAQEAAMKGELRADLLEAKIKEIS
                     ALPRPERYAERLTIDIELKKLKARLQIAKK"
     gene            complement(29013..30425)
                     /gene="atpD"
                     /locus_tag="PLJ75_01125"
     CDS             complement(29013..30425)
                     /gene="atpD"
                     /locus_tag="PLJ75_01125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPD03853.1"
                     /translation="MAENIGKVVQVIGSTLDAEFQENKLPAIYNALKLEVEVMDERKT
                     LVCEVAQHLGGNRVRAVALGSTDGVKRGASIIDTGAPITVPVGEETLGRVFNLLGDVV
                     DKGVPITVKERRSIHQEPPKFDQLEPKAEIFETGIKVVDLIEPYIKGGKTGLFGGAGV
                     GKTVVIMELINNVAMQHGGYSVFAGVGERTREGNDLWLEMKESGVINKACLVYGQMNE
                     PPGARLRVALSALTMCEYFRDLSGRDVLLFIDNIFRFSQAGSEVSALLGRMPSAVGYQ
                     PTLATEMGELQERITSTKRGSITSVQAIYVPADDLTDPAPATAFIHLDASTVLSRQIA
                     EKGIYPAVDPLESSSRILSPEIVGEEHYSVATEVKRILQRYKDLQDIIAILGMDELSE
                     EDKLLVQRARKIERFLSQPFFVAEQFTGMAGKYVKLEETIKSFKGLINGEYDHLPEQA
                     FYMVGTIEEAVEKAKKLAGN"
     gene            complement(30472..31356)
                     /gene="atpG"
                     /locus_tag="PLJ75_01130"
     CDS             complement(30472..31356)
                     /gene="atpG"
                     /locus_tag="PLJ75_01130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPD03854.1"
                     /translation="MATQREILKRINSVKNTQKITRTMEMVSTAKMKKMQDRIVMSKP
                     YSEKLETIIEHLRQTGVEDVFDPLMQERPDPNRILILMITGNRGLCGGFNTRIIENTI
                     GFKSKLTIEEGKEVLVYSIGKKGTNYLRFIKEPMFKSVLNPEDKFKFDDASKLGEELI
                     NLFVTGEVDEVYVSYTKIMTRSTQKATISRLLPLKPEGGAIQESVEEFHVEYIFEPNP
                     YKIFSSLLPLYIKMKLYTAVLETGFSENFARRVAMKNATDAAKEMVRDLTITYNRARQ
                     AKITKEISEIVGGAAALE"
     gene            complement(31380..32909)
                     /gene="atpA"
                     /locus_tag="PLJ75_01135"
     CDS             complement(31380..32909)
                     /gene="atpA"
                     /locus_tag="PLJ75_01135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPD03855.1"
                     /translation="MKIKTEEIKSIIKKEIEGYKSQLDVSEVGTVIQVGDGIARIYGL
                     DNAMAGEMLEFENGAYGLVFNLEENSIGAVILGDYLSIEEGDTVKRLNRVLAVPVGPE
                     LLGRVVNPLGIPLDNKGPINTTKMRPVEFLAPGIADRQPVKEPLQTGIKAIDSMTPIG
                     RGQRELIIGDRKTGKTAIAIDTIINQKGKGVYCIYVAIGQKASTVASVVEKLEQYGAM
                     DYTIVVTASASDPAPLQYIAPYAGCAMGEYFMYEKNGHSLCIYDDLSKQANGYRQLSL
                     LLRRPPGREAYPGDIFYCHSRLLERAAKLSDDLGGGSLTALPIIETQEGEVSAYIPTN
                     VISITDGQIYLVPGLFASGVRPAVDVGISVSRVGGNAQIKAMKKYAGSLRLDLAQFRE
                     LEAFAQLGTELDPATQRQLDRGQRLVEVLKQVQYEPMPVAEQVAIIYAGTQGYLDKVP
                     VPEVKRFEKEFLKFLRDAHPELLDGISSTGELKDPDKFGKVAGDFVESYLAGKKADVR
                     A"
     gene            complement(32899..33468)
                     /gene="atpH"
                     /locus_tag="PLJ75_01140"
     CDS             complement(32899..33468)
                     /gene="atpH"
                     /locus_tag="PLJ75_01140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPD03856.1"
                     /translation="MATSEVAKVYAGALLEIAHEKNAIAKVEEELSFLAELLKNDPDL
                     LRYFNAPGINNDSKKQFVDKVFGGKLSDLMVNFLKVLIDNGRQQDIPDINVAFGELLD
                     IINNRQKIKLISSIKLDADIINRIKKSLEDRFNKNIIIEEEVIPSILGGIIVQVDDLI
                     IDASLKKDLIKLREKLIQSKIRGEVAYED"
     gene            complement(33510..34019)
                     /gene="atpF"
                     /locus_tag="PLJ75_01145"
     CDS             complement(33510..34019)
                     /gene="atpF"
                     /locus_tag="PLJ75_01145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPD03857.1"
                     /translation="MEVLKEGLLKVEPGLLLWTIITFIVLLLILWKAAWKPIVEALDA
                     RAEKIRGDIESAEKSRLEAERLFAEHKAMMDKAKEEAASIIAEGKADAERLRNSIVEK
                     ANQEAKDLIERARREINLAKDKALAEIQSEVVTISTDIAAKIIAKNLKVEDQKALVEE
                     ALQKIRTVQ"
     gene            complement(34042..34326)
                     /gene="atpE"
                     /locus_tag="PLJ75_01150"
     CDS             complement(34042..34326)
                     /gene="atpE"
                     /locus_tag="PLJ75_01150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPD03858.1"
                     /translation="MEYLGLSYLAAGFGAGLIVFGAALGIGKLATGALEGMARQPELS
                     GDLRTAMIIAAALIEGFTFFALVVTFMLATKAPIATTPVEKPQAEQTEVK"
     gene            complement(34416..35273)
                     /gene="atpB"
                     /locus_tag="PLJ75_01155"
     CDS             complement(34416..35273)
                     /gene="atpB"
                     /locus_tag="PLJ75_01155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPD03859.1"
                     /translation="MIQLLQSLPVIIQSSKSGEEESMYDIVMHHLTDHPIHDGFIGKI
                     NNLFFHEKLFGIFDMTITRWVIMLWIAALLCLLIFIPVARKIKNSVYGSRSRWVNLWE
                     VLIAFIHDEVVEPNFDHHYVKKAMPYFLTVFFFILFCNLIGLVPGMSTATGNLAVTGG
                     LALCTLIGMLGVGMVKQGIFGYWVGLVPHGVPAFVLPLMYPIEIIGLLIKPFALTVRL
                     FANMTAGHVVIIIFIYLVMMFQSYWVGIGSVAGSLMIYLLELLVAFIQAYIFAVLSAM
                     FIGTSMHAH"
     gene            complement(35328..35600)
                     /locus_tag="PLJ75_01160"
     CDS             complement(35328..35600)
                     /locus_tag="PLJ75_01160"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPD03860.1"
                     /translation="MKKDVTRIILGYSVIGIQLAITILVFLYGGFFLDQHFNSSPLFV
                     VIGTVLGMGIGFYNLMKSLSELDRLLKRSQQADDSNSSGSKRRKWL"
     gene            35847..36098
                     /locus_tag="PLJ75_01165"
     CDS             35847..36098
                     /locus_tag="PLJ75_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03861.1"
                     /translation="MKLSIKLIENNNEFIATCPELDISCYATSKHEAIKRIQNILFFY
                     MQSAQELGFSVENFETIDVDGKHYLLRYDICPTTNSTLH"
     gene            complement(36434..37819)
                     /locus_tag="PLJ75_01170"
     CDS             complement(36434..37819)
                     /locus_tag="PLJ75_01170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPD03862.1"
                     /translation="MKKLIAASFVIIIIFAYIGCKCNSDILARYGDTTITRGQFKQWL
                     KDKQYPIETILKNKKQQRDKLELMAIESIALKEMQKDNLQASEKLKFLEDMAFESVLL
                     KALYDKKIKKNTDFNEPAFHIKQIFLRVRDFKIVNNKRVNLTKSELEKAYGDVENKAR
                     EIIGRLKNGEKFEELAKQFSDDFSKKNGGDIGYIVKDMIQSSLYQAINESNIGLLETP
                     IRAENGIYIIQIVEKQNITKKNLNKIIKDEMQAKRLEQILLRKASNNYIDNLMSAQDV
                     QFNEQKCKSKNPKDIIFKVGDTIFTVGDLEKKISYFKHDQPVFHKSVMEDTKKVDIAK
                     NAFKVALLRRAAIKENMHNDEEFKKEVEKRKQMLFAKEYMDYIADRDVIVTPKEVRDE
                     YEKYKDTRFTTYTNQKGKTEKQIISFDKVKDQIAMMIYRKKRADNLAKWKRDMLHIYK
                     FSVNEAELEGK"
     gene            38080..38613
                     /locus_tag="PLJ75_01175"
     CDS             38080..38613
                     /locus_tag="PLJ75_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD03863.1"
                     /translation="MRDKAFYKNRLFSLIHHDAPIDCKTLIEYGYDAIEKGNFIKAFR
                     LFMLGYRLIGNNAEILNGMALSLCELGRLKFSKSILLFALKKFPDNVLLLTNLATVYF
                     DEYNYDKAIFYYTKAISIDPFFIEAHINLVNVYCERGDIFMAYIACLNLLKKHPDNFE
                     AQELCDDIIINMGLSLV"
     gene            complement(38610..39020)
                     /locus_tag="PLJ75_01180"
     CDS             complement(38610..39020)
                     /locus_tag="PLJ75_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03864.1"
                     /translation="MKNLSYTIKKYLLFLLIIMLSCSKAPVDDAANVVINYLATTDEK
                     KLHGYLDRDSIQYIETLKKDAGTMYPYVWVHHDNNAIWDVVAVSRTANEAWVTVQCVK
                     HKKLNAIGMTIKHKLIKENGEWKISLKGELPKND"
     gene            complement(39151..39327)
                     /locus_tag="PLJ75_01185"
     CDS             complement(39151..39327)
                     /locus_tag="PLJ75_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03865.1"
                     /translation="MLSKVKAMDCITELPYHHEHSEKSHRFDNVFHSERSEESHHFMK
                     EEILWDYVPQNDKK"
     gene            complement(39418..40617)
                     /locus_tag="PLJ75_01190"
     CDS             complement(39418..40617)
                     /locus_tag="PLJ75_01190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPD03866.1"
                     /translation="MKLRPAYKRVGMLFSGGPAPSANTVISSVALNFLDNGIPVIGFY
                     RGYEFLQDFNINYPKIKKGVHFEEIDYSITHVRNERGIYLKTSRANPGKDIKTIQDIK
                     DSEKVKKLNNILDAFEYLKIGALISIGGDDTLKTANYLNLLGFPVIHIPKTIDNDYYG
                     IPWTFGYWTAVDTAQKSILNLKADAQSTDSFFIVELMGRKAGWITYAAGIAAEAIMMI
                     AAEDIEEDMLDLEVLVDKITNVILARENNRRPYGVIAVSEGIVDKLSDEYKPKHTDKH
                     GNVLYREAKIGEILAERVKKRYQEKTGMEVKIIAKQIGYETRSAPPISFDVVLGAMLG
                     YGAYKFYNETMFGIMVSVTDNFDLKAVPFSELIDQDTLKTRLRDVPKGSDFYTLKERL
                     SYRKSWE"
     gene            complement(40703..41377)
                     /locus_tag="PLJ75_01195"
     CDS             complement(40703..41377)
                     /locus_tag="PLJ75_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD03867.1"
                     /translation="MREILNQAIAKRQKALSEFDSKQLMKMAGIPVSRNYLAKSKEEA
                     LDYAQKLGFPVVLKGCSDTLTHKTELGMVKLKLKTKEDVANAYDEITGKGIPLDGVLV
                     QEWLDGDNREFVLGSIKDPQFGPCVMFGLGGVFTEALQDVSFRVAPITSIDAYEMMDE
                     LRTKKLLGPFRGSPAVDKEMLARALVGLSELINTYEEIAEIDINPIIINGDKPVAVDA
                     LVILKQ"
     gene            complement(41370..42836)
                     /locus_tag="PLJ75_01200"
     CDS             complement(41370..42836)
                     /locus_tag="PLJ75_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPD03868.1"
                     /translation="MIESLFNPGSVAVVGASEVPLKWGTYIASNILDGKFNGRFYPVN
                     PNVPEVFGYKTYPSLLDIPEPVDLVFITTPSKTIMHILQDCIEKGITNIVMITSGFSE
                     TGKEGAMLENDIVALAQSRGLHIVGPNTMGIVNTHCSLYATGTITRPKPGGISIIAQS
                     GNLGFQILEWARSQNIGISKFVGSGNEAVLKIEDYLEYYKSDSETKVILMYIEGIDDG
                     KSFVEIAKNTSLKKPIIALKAGRTEIGSKAAASHTGALAGSYSVFKGVVQQTGIVLAE
                     SPKELLSLSAAFDSFPLPKGNRIGIVTLGGGWGVITADECVERGLLIPELPQSIVEEL
                     DKRLPPFWSRGNPVDLVGQPDFQIFKDAVELMVANDAFDVVIVLGIIGAKVFALRAAE
                     SILNLGKMEKSIYDDFMKSLNVLEKEFLDRLTELMAIYNKPIVTVSFSKSFEDETIHD
                     CGGRFKCVIYDTPEEAVLCVAKMYNYYNYVTKRSMVYA"
     gene            complement(42926..43912)
                     /gene="galE"
                     /locus_tag="PLJ75_01205"
     CDS             complement(42926..43912)
                     /gene="galE"
                     /locus_tag="PLJ75_01205"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPD03869.1"
                     /translation="MKSILVTGGAGYIGSHVLRLLAKNGFNPVVYDNLSGGFKDFVSG
                     FDFIEGDIGDFDKVMFVLKKYDIQCVMNFASFIAVGESVQNPLKYYENNVAKTLTLFK
                     AMLQSNIKQFIFSSSAAVYGNPDVVPIPEESPMKPESPYGKTKYCIEEVLKDLTASDS
                     FHAMSLRYFNAAGADESGQIGESHIPETHLIPLVIRAVLDPGYTVTIYGTDYDTPDGT
                     CIRDYIHVNDLGDAHVLALKKLIETNQSDVFNLGNGKGFSVKEVIASVRRVTGRDFKV
                     QEGQRRKGDPAILVASSKKVKLQLQWQPHYTHIDSIVATAWKWESQQKRKGY"
     gene            complement(44136..45074)
                     /locus_tag="PLJ75_01210"
     CDS             complement(44136..45074)
                     /locus_tag="PLJ75_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD03870.1"
                     /translation="MYTYLFGPVPSRRLGISLGVDLIPHKTCTLNCIYCECGKTTKLT
                     IERKEWVPINDVIDELKRYLNENPCPEYITFSGSGEPTLHTGIGIILDFLKEQHGTFK
                     TAVLTNGTLLYMPQVREELLGADVVIPSLDAATQETFKKINMPHPSLNIQTIINGITE
                     FKKIFKGKLILEIFIIPEINDNNNDLDALKHAILTIHPDEVQLNTLDRPGILSSIRAA
                     TMDELEHIIQYWQLPNVSIISSPSSHIDKVHTAYRYDIESAILETIARRPCTVNDIAQ
                     IININSDELNKYLDDLEAQRKITRVVQNRGIFYKKV"
     gene            complement(45076..46095)
                     /locus_tag="PLJ75_01215"
     CDS             complement(45076..46095)
                     /locus_tag="PLJ75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HPD03871.1"
                     /translation="MHQIIDIIKESLFITMFVVVIMLLVEYINVVTQGKITNLKSKKL
                     QQYCIAIVLGIIPGCLGGFAATAMYAHGAISLGAIVATMLATSGDEAFVMFAMFPKTA
                     LLMNIGLAIIAIAAGYIVDIIFKQHSNSEQCCDGLVIHNEVSFSSYTLHNIAALWKNL
                     SLSRASLMSVLVIIFFMIVTGVFHEEEGFVKVAVPIIVVIAMIIVAIVPEHFLQEHLW
                     EHVFKRHLPRIFLWTVSALFVIHVLVNILHIDASIHHAKWMVMIIAGLVGIIPESGPH
                     LLFVMMFAKGLVPLSVLVTSSIVQDGHAMLPLLAQSRKYFVLIKFINLIIGLFIGSVM
                     MALGY"
     gene            46498..46899
                     /locus_tag="PLJ75_01220"
     CDS             46498..46899
                     /locus_tag="PLJ75_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03872.1"
                     /translation="MKLQSLKHFVACLFLLPVLMLSCSDEGITIKFMVVSTVKGFDGY
                     YIVDGDSVGYFSATENAYGIAMYEKEIDDVEYLEVSATTRRDATSIEIKVYRDDKKVK
                     SSQTNIEDPFEPTILNLEYSIGEEDEEKTSS"
     gene            46924..47397
                     /locus_tag="PLJ75_01225"
     CDS             46924..47397
                     /locus_tag="PLJ75_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPD03873.1"
                     /translation="MLNFEIHDATRELSIARALKKYEPPFKVIDSYRLIDDGSNPEAT
                     VQIEANGRYIHEASTGDGPVDALATVLKKALIPLFPEIENVKLIDYRANILDAKRGTS
                     SNVEVTIIFTDGEHVWKVYALSENINAASFRVLLDGFEFAILKKNKKTINKSQEL"
     gene            complement(47375..48529)
                     /locus_tag="PLJ75_01230"
     CDS             complement(47375..48529)
                     /locus_tag="PLJ75_01230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD03874.1"
                     /translation="MKKTLSAIDQAVLIRAIEQSPASIVITDTRGIIQYVNPKFESIT
                     GYSFTEAIGQNPRILKSGHQPKEFYQNMWETILRGEVWDGIFHNKQKNGSYFWEHCII
                     APVADEHGNVTHFVAIKEDITEKKEQEEALRKSEEELRKRNDIINKDLEYAKRVQQSI
                     INTPIPEYPGIKIVTCYRPYDFVGGDFYTIKKINHDLAVFMADITGHGVPAALFSSVL
                     KYATENLWNNGIIQPDEFIHQLNEKLFPILGGYYFTALYGLITPLLDGGFKLTYTRSG
                     HPYPVVLSGDAIAMLDSSGIPLGIEKNVKLLVKNIELKKGDRLFLYTDGLIEIDINNQ
                     KDLRFDILTKEIIATKQIALHDAVPKIIEVVTSQNKTLDDDIAVIALEIC"
     gene            48784..49377
                     /locus_tag="PLJ75_01235"
     CDS             48784..49377
                     /locus_tag="PLJ75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD03875.1"
                     /translation="MRKNNRQHILDTARKLFLEYGYNGISIRNIAKRAKLTTGAIYFH
                     FKNKKEIYSTICLEAINTLHRLFSDGIKLRKTPAQKLISTYDSYLKFYYEFHDYYNVL
                     TEYRAEYDDEPDPYKDEIQKQNLSIQNLMADVFKEGIDQGVFKDLDPKMVAFFLSAVA
                     EGMLQFKKLGVMDAAGITDNKFREFMKIIVGQGIQKK"
     gene            complement(49523..50890)
                     /locus_tag="PLJ75_01240"
     CDS             complement(49523..50890)
                     /locus_tag="PLJ75_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03876.1"
                     /translation="MNTPFTFEAMIAFGWMSVMLLLGIILRAKVKAFQRFLIPSCLIG
                     GAIGLILRSYEIIPLSADILETLAFHLFNISFISIGLTYNKEKADDKGVGEVLHGSLW
                     MANIEGVSFSIQAIIGGLCVMVLNYLGYNLFNSFGFLTPIGFEEGPGQALSVGKVWEN
                     LGYAHSATLGLTFAVAGYMFSFFIGVPLAYLGIRKGKAVYTPKELSMELRRGVMAQQS
                     EKEVAGELTTHSSNVDALAFQASLVGVVYMITYVVIYGISSLLSPEMGQMMWGFFFFI
                     GLLIAFIVRFIITKLGGLYLIDPGIQHRITGFSVDYLLVATVMAIQVVVVWQYIVPIV
                     VICVLAGIITTIVCYYYGRRLWSVNLERMLLIYGTVTGTVSTGLLLLRIADPEFKTTT
                     AIEAGIMSIFGAPYVLLGTLLVSAPILWGWSIVKTILVFAGMLIVAAAIIELQRYLLL
                     KKMKQ"
     gene            51195..51953
                     /locus_tag="PLJ75_01245"
     CDS             51195..51953
                     /locus_tag="PLJ75_01245"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPD03877.1"
                     /translation="MKIIDLSSIIEPSPSGTPDFLKIDIKYHSHGDGAKQVEALLKVP
                     AHLMRNGEGWATETITQLGTHDSTHVDAPWHYNSTIQGKPAPTIDELPLEWFYGNGVK
                     LDMTHKAEGDPVTPQDIEKELKRINYTIQPNDIVLMYTGMDKFYGQPDYIFRGCGVTY
                     EATVMLYNAGVRVMGIDAWGWDMPLNLQAQKALQENKPGIFWSAHQADLIYSHMERLV
                     NLGLLPAKGFKVACFPLNIKRASAAPARVVAIVE"
     gene            52150..52938
                     /locus_tag="PLJ75_01250"
     CDS             52150..52938
                     /locus_tag="PLJ75_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPD03878.1"
                     /translation="MGDTTQNKCHYIIENGIAIITIDNPPMNALNHIVLKDLRNSVNA
                     LIDDAAVRVIIITGAGKAFVAGADINEIKVQNTKEDGANFLKNGQEIYNIIENADKPF
                     IAAINGYCLGGGLELALACHIRLADESAQVGLPEIKLGIIPGYGGTQRSPRLFGKAAA
                     YELILSGNFISGKQAETYRIVNRATQKGTVLKEAQELAKAIAARSRLAVKAAMQAIAQ
                     GYTMEFYTALTFERELFGKLCETEDKKEGIAAFLEKREPQFKDK"
     gene            52943..53389
                     /gene="rimI"
                     /locus_tag="PLJ75_01255"
     CDS             52943..53389
                     /gene="rimI"
                     /locus_tag="PLJ75_01255"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPD03879.1"
                     /translation="MDNLIIRHAHYDELEAIARLDALVFGQNAWPFNSFVNELQLHFS
                     KLLIAELDTTFAGFALAWYIADEIQLMRIAVNPVFQRNKIGSTLIQHITHYAQNQSKI
                     VLEVADNNTQAIAFYTSLGFYRVGVRKNYYHLNSAILMEKIIDHED"
     gene            53379..53957
                     /gene="yihA"
                     /locus_tag="PLJ75_01260"
     CDS             53379..53957
                     /gene="yihA"
                     /locus_tag="PLJ75_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPD03880.1"
                     /translation="MKINTVQFITSATTPNHYPKYSFPEFAFIGRSNVGKSSLLNTIL
                     GRKNIAKTGSKPGVTQTINFYCINDAISLADCPGFGYAKISQSKRREFITMIDNYINI
                     RSSLKLVFLLIDIRREPDEHEINIINKSLHKNINVMIVATKCDKVSKNEINAGIKRIC
                     TSLNLHPESVIPSSKTGMGKNIILSVISKHCQ"
     gene            complement(53966..54565)
                     /locus_tag="PLJ75_01265"
     CDS             complement(53966..54565)
                     /locus_tag="PLJ75_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03881.1"
                     /translation="MKLVSDSTKVVYLILMIVFIVIVGLFWMDYIGLINLGKIYHHYI
                     VTEAPSVVDAADDEPSLIEREEFEKEKDKLLQRIEELDKREAKITEAEKVISKEKEKL
                     EQLQKGLDQEKKALELEKKKYSGYKKNVMDLAKKIESIPPQDAVDIMVQWEETLIIDV
                     LRQIDQNAAKEGRMSISTYLLSLMPKEKASRVMYLMTQL"
     gene            complement(54598..54948)
                     /locus_tag="PLJ75_01270"
     CDS             complement(54598..54948)
                     /locus_tag="PLJ75_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03882.1"
                     /translation="MDINSIQQMQKRIAEIKSRFAAIQKTYNRISPSEKTTVQNTPNA
                     MQQNTNQQPDIKGTIEQKNNTFDNKITEEILKSVLYNKADSKASSDMQQIQNLLKKAI
                     SQYTSKGDLFNIEE"
     gene            complement(54956..55399)
                     /gene="fliJ"
                     /locus_tag="PLJ75_01275"
     CDS             complement(54956..55399)
                     /gene="fliJ"
                     /locus_tag="PLJ75_01275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HPD03883.1"
                     /translation="MKKFQFRLQTLLDIRISKEHAIKAELAMLIAKQNEEIVMQEEYR
                     KKIKEYYNKLSKKMRDGTFSYNEALMCERFAYNAQKAIDLSKKRVEQMQPEIDRVRQK
                     LMQASKERKVVERLKEKKYEEYMYQFNREMGKELDDLNQRIYNAM"
     gene            complement(55423..56829)
                     /locus_tag="PLJ75_01280"
     CDS             complement(55423..56829)
                     /locus_tag="PLJ75_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HPD03884.1"
                     /translation="MIQILPHEKVDIFTKYKEVIEEVETIRATGRVEKITGLTIESHG
                     PDVQYGELCRIRLKDGGYLNAEVVGFNKNKIILMPIGDMKGVVPGADVYAVGSQLIVP
                     VGDELVGRVIDGMGNPIDGKGNIYLKEGYPVDNKPLRPLEREIIDSPLSTGIRAIDGL
                     ITVGKGQRIGIFSGSGVGKSTLISMIARNTAADISVIALIGERGREAKDFVEKELQNE
                     GLKRSVVIISTSDQPPMLRLRAGFLAHSVAEYFRDKGLHVNLLMDSITRFAMAQREVG
                     LAAGQPAATRGYPPSVFSLLPRLLERAGNKKCAGSITGFYSILVEADDMNEPIADAVR
                     GILDGHIVLDRKLAHKNHYPAIDVLGSISRCMNDVIDEEHKKAANKFRTLLAAYHEAE
                     DLIMLGAYARGSDPDVDAAIDMKPSMDEFLKQGIYEKDIYENIKTKLIELMFGSKVKQ
                     EQKRDFVSREPYFVQRNR"
     gene            complement(56863..57789)
                     /gene="fliH"
                     /locus_tag="PLJ75_01285"
     CDS             complement(56863..57789)
                     /gene="fliH"
                     /locus_tag="PLJ75_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HPD03885.1"
                     /translation="MSKLVFKPAEIQITATPKQIELPDKYQKNILTDEEYAEFEVDEQ
                     GNPVEVYQGPSIEEMEAELERYRRETEEEVRRMLDEAKARAAQIEEDGKKAAFLELQQ
                     AREQIKIEMERLKIESEREVERGKFEAEKMIKEAELKVSEIEHEAYKRGYDAGREEGY
                     KEGQAEVMRLIDRLGTIVSTAVDIRDDIIRSSEKLMTEMILMIARKVIKDEIVERREV
                     VINNIKEALQRVKDRDRIDIRVNFADLDMTTAHKDELIKMMESLKKVNIYEDSRVERG
                     GCIIETDVGAIDARISTQLEAIEEAIRNARPL"
     gene            complement(57782..58798)
                     /gene="fliG"
                     /locus_tag="PLJ75_01290"
     CDS             complement(57782..58798)
                     /gene="fliG"
                     /locus_tag="PLJ75_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPD03886.1"
                     /translation="MLQQKKSQLTGRQKAAIFLVSLGSDVSSEIFKHLREDEIEQLTF
                     EIARLDKVEPEDRDKVLMEFQELMMAQEFIATGGIDYAREVLERALGTQKAIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQGEHPQTIALILAYLDPQKAATILSGLNHQIQAD
                     VARRIATMDRTSPDVLREVERVLERKLSTLASEDYTSAGGIDSIVEVLTHVDRGTEKI
                     IIEALEEEDPELAEEIKKRMFVFEDIVLLDDRAIQKVLREVDTQDLAKALKGVDAEVQ
                     EKIFRNMSKRASALLREDMDFMGPIRLRDVEEAQQKIVNIIRKLEEAGDIVVARAGEE
                     ELVV"
     gene            complement(58937..60634)
                     /gene="fliF"
                     /locus_tag="PLJ75_01295"
     CDS             complement(58937..60634)
                     /gene="fliF"
                     /locus_tag="PLJ75_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HPD03887.1"
                     /translation="MDLFTKIFQQLKEIYAKLDTTKKIIIGVVTGVLVIAFIVLFSVS
                     SQKANIVLFSDLPSDEFGQVTKKLEEMGFHYTTSGTTTILVDPNQREVILTRLAQENM
                     IPRGIPGWKLFDISKWTETDKELNVKYMRALRDEIKRHIESLKNIEKADVEIAITEDE
                     LFTEKENAYTAAVTVYLAPGYDTLSKKEIKGIQYLVSRAVGTKLKPENVSITDEYGKI
                     ISDFDDQFDKAKEEFTLLEYRKKIEEKARVQMLKDIRLGLEKIFSSDRIQIVRLNMDF
                     NWDKIQEEQEEYTPIEMEKDNPLTPYSERKVKDALTISKKSTKEHFQGHGWNPEGPAG
                     TEGNKPPGYKASDDQFSQYDKEENVENNAINKTIRKIERDPFDISAVSVAIAIDGYQD
                     LPRLPDGSYDLDPAKKPVQLPLSEDELRQAENIVKKAINFNAARGDQVAVENIMFDRT
                     KQWDIAREEFRKKEQFKRMLLAALVGIMALFLGVILFRAISRELARRRRIREEQLALE
                     QQRMREAALRAAEEEGIDVELSLEEKARLELQQNAVNLARERPDDVAQLLRTWLAEE"
     gene            complement(60861..61190)
                     /gene="fliE"
                     /locus_tag="PLJ75_01300"
     CDS             complement(60861..61190)
                     /gene="fliE"
                     /locus_tag="PLJ75_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HPD03888.1"
                     /translation="MVQESNFISKSNIFTMKTTHPLHYDNKPEKIQDDDVAGSFMDAL
                     KSTLGKVNDLQVDAEDLTQQMIYEPESVDIHTVMIAQQKAEIALSLTKAVRDEAIRAY
                     RELINLR"
     gene            complement(61174..61344)
                     /locus_tag="PLJ75_01305"
     CDS             complement(61174..61344)
                     /locus_tag="PLJ75_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03889.1"
                     /translation="MYFEKGHINEICCSHELSTPITLSRRKKEWLQFGIGERQIVESR
                     IISGSVNYGTGK"
     gene            complement(61368..61832)
                     /gene="flgC"
                     /locus_tag="PLJ75_01310"
     CDS             complement(61368..61832)
                     /gene="flgC"
                     /locus_tag="PLJ75_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HPD03890.1"
                     /translation="MGMFDSFNIASTGLTAQRLRMDVISNNIANATTTRTPEGGPFKR
                     KRVIFAPVNIRPFYKSPLVPKRIDHGEPKGVRVVKIEEDNAEFRLIYDPAHPDAIKTG
                     PKQGYVEMPNVNVVMEMTDLISASRSYEANVMMINNAKSMFSKALEIGKGGV"
     gene            complement(61845..62273)
                     /gene="flgB"
                     /locus_tag="PLJ75_01315"
     CDS             complement(61845..62273)
                     /gene="flgB"
                     /locus_tag="PLJ75_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HPD03891.1"
                     /translation="MFEYSKMMQTNYLLEQAMDVESIRRKIIANNIANVDVPHFKRSE
                     VNFESELKRAIEHNKDSMQKVKALKTDPRHFDFFEPKNIQSVKPRINVDYTTTYRNDG
                     NNVDVEKEMVDAAKNQMRYNAYISSLNENYKILKMVMRTV"
BASE COUNT        19812 a        12425 c         9716 g        20323 t
ORIGIN      
        1 ctcttatttt tctattgcaa gcattttttt aaagatttgt tcagaggtct caattgtaaa
       61 cagcaggtat aacatcaatt cttctgtttg aaatgaaaat catttatgat tcccctgcat
      121 taaaaagctg atgtcaagca atttgagcgc ccatcatcat aattaatgca tgttatgata
      181 cttttccata aatgtccgag gattaattat cctggacttt ttaatgattt ttaattccaa
      241 caaatcttta tcacctgtta taattaaatc agcatcaata tattgagcaa gatataaaat
      301 attgttatca tctttatctt tacataaatc tggcatttca ccttcaggtt gaataaaacg
      361 aaatgcagtt aataaaaaat cctttactcg cttaagttct tttttaggag ttttaaattt
      421 cgtatttaat ttttcaatta cttcattaac tatccattgg gagatgaaaa gttgatgctt
      481 atcaatacaa atatctataa ctcgagaaga taatccttga gtaataaaag cagataaaat
      541 gacattagta tctaatacaa ttttcatgaa aaatctttaa atatgtcttc atcagtaaaa
      601 taacctgcct tttcgccaaa gggtatcaac attgatctaa tttcttgaaa ttcctcatgt
      661 aagatatact tttcaattgc ttttttgact aattcactct ttgaaacatt aaatttttca
      721 gcagcttttt cgactttttc ttttaatttt gagtcaatac ttattgtcaa tatttctctc
      781 atatacttca ccagtttaaa taatttatag ttcagtgtaa taaataatat tacagaagtc
      841 aataaatatt ttgtttttga agacattata gctggtttca ccattttact gtgtggcatt
      901 ggcattcagt ttttaggatt ataaaagttg taatacatcc cggttataat cttagaaaat
      961 ttttttccaa acagcataaa acatcttgtc aggaaacaaa tgaacatgaa aaggtatact
     1021 attattcatc tttttttata ttatcctttc tgaattgatt tggcgaaaca cctgtcatct
     1081 tagaaaaaac cgtacaaaaa gttgtattgg aattaaaccc aactgcaaaa ccaatcgaaa
     1141 tgattgaacg atctggttca tcaactaaaa gcttttgagc ttcttttacc ctgaattcat
     1201 tcacaaatgt attaaaattc ttcttaatat gctcatttaa aatttcagaa agctgatgta
     1261 ctgttatacc taaatctcgt gccaggtcag gcaggctaag ctcttcatca gcaaatgcct
     1321 tttcatctac cattatctca taaagccgtt gtataacact atcaacatcc aatcctttga
     1381 ttcgtgaacg ctcataacgt gcttttaagg cttcattctt tagcaactta ttataatcgg
     1441 gaagacgctg tgttaccaga tatacaaacg tcatggctgt attggctaaa attactgccc
     1501 actcaaccac ccttaacgat ataaacactc ctataacata tacaatattt gaaataacta
     1561 ttgacatcgc aaagatgtat ccccagcgtg aaacgcctgg tactttgttc caattgctct
     1621 ttttccatat atacgacgtc ataaaaaggt tctgcgacat aaatgaaatg agatagatat
     1681 tggggaagat aaaaaacagg tacaatattg tatcatagag cgatagttca ttaaatgtat
     1741 ggctaagaat gggcaatgca ggaaaacagg tatatccaca gtcactatcg ataaaaaatt
     1801 ggatcattat atatagtaac gaaaccacta ctggtgaaaa taagaggtaa tatttttttt
     1861 gaaagttaaa caaacttgac attatccatg tatagcgtat taccataagc ggcggaacgg
     1921 taaatacaat gggtatggaa aagatacaca catagtaacc aacggcagtg ccatcaaata
     1981 accctgaaga aaatatggaa atctgtaaca accaaatccc cagccctaaa aatgaaaatg
     2041 cataaatcct gttgatggtt tcacttttct tttgtaaaag ctgacctata gcaataatca
     2101 ccgaaaacgc agcgccataa aacataaaaa aatgtaaaat agactcagcc atatattact
     2161 caaaatttta tagttataac attgaaatat agcgaacttt caattgtata cacttgaaag
     2221 atttcaggca taatatttga tataatcata acagttattg aataccatcc agtcgaaata
     2281 attgatatgg atgagatgaa actatcggag gtaattatat ggctcagcat gaaaatgtca
     2341 attatgaaaa agaatttgaa gaatatttaa aacgccagaa gcaccttgga ctcatggcac
     2401 ttgatcgtgc tgaaaagttt ggtgattcaa agattgcagt gcgtcataaa gcgtatggca
     2461 catgggaagc atttacatgg aagcagtttg gtgatttaat aaaagctgct gcaaaaggtt
     2521 tgctatctct gggcgttaag gagtttgagt ttgttggtat tttttcaaac aacagagttg
     2581 aatgggctgt tgtagattat gcatgcttta ccatcagggc atgttctgta ccggtgtatg
     2641 ctaccaactc agccaaagaa ttggaatata ttgtcaacga ttgcggcatc cggattcttt
     2701 ttgttgaaaa acaggaacac tatgacaagg caatgagtgt atttgacaat tgcaaaacgc
     2761 tggaaaagat tatagtgttt gatagaaata ctaacatagt caacagtgat aaagtaatgc
     2821 tctttgatga ttttattcaa ttagggaaaa attctggatt ggatgctcag ctacaggagc
     2881 gattatcaaa agtacagagc gatgatcttt ccaccctgat ttatacatca ggaactaccg
     2941 gcgagccaaa aggggttatg cttacccata aaaattggtt tgcaatgctg tttggtacag
     3001 gataccatat taccgttgaa cccactgatg taaaccttgc atttttacct ttatcacatg
     3061 tttttgagcg tgcatggagt tattttatat tgtgcaacaa tgcgcaggtt gattactgcc
     3121 acgatacaaa agcattactg gattttctga aagaatctcg tccccatttc atgtgcagcg
     3181 ttccacgaat gtgggaaaaa attcatgcta ccatcatgga tggtattaag aattcaccac
     3241 ctgctaaaca aaagatattc aactgggctc ttgaggttgg cggcaactat cagtatttaa
     3301 aaaaagataa taaatccatc ccactgggat taaggctaaa acatgccata gccaccaaac
     3361 tggtactgca taaaatcaga gatatttttg gtggaaggaa taaagtattt aattgcggtg
     3421 gttctgcctt ttctggtgaa atcagcgaat ttttctttaa agcgggtgta ttcattttac
     3481 agggatatgg tttaaccgaa tgttttgtga tatgtgtatc caatccacat cgcaataaat
     3541 ttggcacctg cggcccggtt gttcccctga tgcaggtacg aatctcagac gaaggtgaaa
     3601 tacaggctaa gggccccagc atgacacagg gatattggaa taaaccagaa ctcacaaaag
     3661 ccctttttac accggatgga tgggtaaaaa ctggagatgt tggatttatt gacagcgacg
     3721 gattcattac cgtaaccgac cgacttaaag acctattcaa aacatcaggt ggtaaataca
     3781 tagctcctca gcaaattgaa accttactca atgaagattt ttattttgaa caggttgctg
     3841 caattggtga tggcagaaaa tttgtatctg cattaattgt tcctgccttt gagccactta
     3901 aaaattgggc taaaagtaat aatattccat tcacatcaca cgaagacctt atcaaacatc
     3961 ccgcagttat acaattttat cgggaaagga ttgatgaatt aacaaaaaat ttgggtcagg
     4021 ttgaaaaaat taagaagttt actttacttc ccaatgaatt tactcaggaa aaaggcgaaa
     4081 taaccccaac acagaagata cgcagaaaag taatcaatga gcattacaaa gatatcattg
     4141 aacagatgta taaagattaa atcatcttta aaaaagctcc tgtgcacgca ggggcttttt
     4201 tattataaaa ataccagata tcctgataca gtataaatca ttataaatac attgacatat
     4261 ttattgtttt actgtataat tattgacatc ttaacaatct ttcataatgt tatgcatatt
     4321 aatatttata cactggatga aattacaaaa acctatgaaa atatagatga ccatctctcg
     4381 acacgacaga ttatccgtaa attttcacaa aacaaaaatg atatacggtt actatcgctt
     4441 gaactatgta acctatcaca ctacaacaat attttagatt tagggtgtgg ttacggcttt
     4501 tttaccgaag cgctcaacaa ccgcatagct tcaggcgctt gcataacagg tgttgatgtc
     4561 attggcggtg aaaacaaacg catgttttta tcaacctgtg tacaaagcgg atacaatgca
     4621 caatttatag aagattctgc acatataatc tgcaatattc ctgataaaac atacgattgc
     4681 attatagcaa gttattcact gtactttttc cctgatttaa taaaccacat cgcacgtatc
     4741 cttacaaatg atggaacatt tatagcactt acccatagtg aattaaccct ttcacaaatc
     4801 acatctattt ttattgaaag tattcaccat gttataccgc taagcgatag ctctctccat
     4861 atagcaaaat tatttaaatc attctctgct gaaaatggtt ttgtgcagct tcagccatac
     4921 tttgattata ttatgtatct gcgatatccc aataatctta tatttaaatc ttcagacatt
     4981 gaccactgta taagctatat acacaaaaaa caatatttat ttttcaagga tattgctttg
     5041 ttagataggg atgaatatga tgcattaatc aatgattttt ataatcgttt acgatggcat
     5101 gtatcatcat ataaggattt tattgtcaac aaggatgatg caatatttat tgctcgcaat
     5161 cccgttaagc acattaatga ataattttat tgatggtaca atacgtgtaa aattgatagt
     5221 gaataaaaag caataaaata ggtattatac catggtgaag tatattttgt tgttttataa
     5281 ggaactcaat tatgaagaaa aaacgcatct actgcagctt ttgcggtgca cccataacgg
     5341 cccgttttat tgatgaaaaa tatcgcgatc actgcgataa ttgcaacact acattctatg
     5401 aaaatccgct tcccgttgca tcatgtattg tcattaataa aaaccgtgaa gtacttcttg
     5461 tgctaaggaa aaacgaacca tataaagata tgtggtgttt accaattggg tttgctgaat
     5521 ccggagaatc aattgaacag gcagctctgc gcgaactaca agaagaagca ggtgttaatg
     5581 gtgagatagt tcgtattatt gatgttgata ctgtttcaaa ctacttttat ggtgatttag
     5641 caatcataac atttgaagta caacaacttt ctgaaacata taaaccgggt gatgatgctg
     5701 ctgatgcaaa atttttttcc cttgataatt acccgcaact ggcatgggaa tccaacgaaa
     5761 aagctttgca taaatttatt gatttatata aagatatatg gacaatgatt gattcattaa
     5821 aaactatcca acctgatata accacccatc atgatatccc cagagaaaaa tcaaaacaat
     5881 atacatttat tgccaacatc attgcatcaa tgattgatga tgaaattgaa ttgtttaccc
     5941 agcagtggaa acgtgagatg cccaatgtta ataataatat ttttcatttt ttgattttta
     6001 tccatcagat ggcgttgcat acaataaaac tatggctaac cggcaatagg atatggaaaa
     6061 atttcaggga attttatatc atgggaaaac agttaaaaaa agaaaatata tccttaaagg
     6121 atatattgag tgccatagct cttagcagaa aatgtatctg gattcaggca attgaaaaaa
     6181 atatattgca ttcaccactg gaagtgtaca caatgctgga gattaataac aggattattt
     6241 tgttttatga taagattagc tattttctta tcaagggata tgaacattca tagcctatta
     6301 ttcacagggc atcccatgag gatgccctgt gttatgtatc ttatgctgat tgtcttcgct
     6361 ttttttcttt gatttttgca agcaccttct cctgcatctc tttaggcagc gcaacatatt
     6421 tatcaaattc cattgcataa tttgcccttc cacttgaaag gcttcgcaat gttgttgcat
     6481 acccaaacat ctccatgagt ggaacaaaac cattgacctt ttgggcaccc tttctaaaac
     6541 gacgcattga ctctacttta ccccttcgcc tattcaaatc accaataaca tcgccaatat
     6601 attcatcagg ggtgttaacc tctattttca tctgtggttc aagcaatata ggtttagctt
     6661 tcataaatgc ttctttaaaa catatcgatg cacatgttct aaatgccata tcagatgaat
     6721 caaccgggtg gaaactacca tcaagcaaca caacccggac atcaacaaca ggaaaatcag
     6781 ccaatatacc ttcttccatt gctgcatgta ttcccttctg aattgaagga ataaattcat
     6841 tgggtattgc tccacctttt atatgatcta taaattcata tcctttcccg ggatttggtt
     6901 ctattcgcat aacacaatgt gcatactgac ccttaccacc ggtttgctta acaaattttg
     6961 tttcctgttc tgcttcagca gttattgttt ctcggaatgc aacggaaggc tcgccaacct
     7021 gtacattaag tccaaattct tctttgagcc tatcaaccat aatctctaaa tggagttcac
     7081 ccatccctga tattatcgtt tcacctgttt cttcgtcaac gcgcacatta aatgatggat
     7141 cttcagtggc taatttatgg agagctatcc ccagcttctc ttcttctttt tttgaagcag
     7201 gcataacctg taaatcaata actgatggtg gaacatatat cgattcaagc aatagcggat
     7261 tatttgtatc acagagggta tcgcctgttt tggtaaattt aagcccaatt aatgctacta
     7321 tctcacccgc tcccgcttct gaaatttctt ctcgttcttt tgcatgtatt cgcatgatac
     7381 gcccaatacg ctcatattta cccttggtag agttatatac ctgcatgcca ctttgtaatt
     7441 ttccagaata tatacgtatg aatgtttgct gaccaacata gagatcgttg attagtttaa
     7501 atgccaatgc agcaaatggt tcatgtgtcg aagggtgacg cgtatgagtt ttttcaggat
     7561 aatcaacatc aataccaact atcgctccac tatcaacagg agatggaaga taatcaacaa
     7621 ctgcatcaag caaaagttga atacctttat ttttatatgc agcaccacaa aatacaggcg
     7681 tgataagcat ctttaatgta gcttctcggg ctgtcttttt tatcagttca gatggcatct
     7741 ctttttctgc aagataaagc tccataatct catcatcaaa atcagccagc ttttcaataa
     7801 tatgtttacg cgcttccttt gcttttcctt tataatcatc cggtatatcc acaatcaacc
     7861 gctcgttttc cctgaaaact atcgcccgtt gctcaacaag atcaatgcag ccttcaaagc
     7921 tgtcttcgac accaatggga atctggaatg gaacagggtt tgcatcaaga tatctatcca
     7981 tctgagaaac aacttcataa aagtcagcac caactctatc cattttattg ataaaagcaa
     8041 ttcgcggaac tttatatcta tcagcttgat tccatacagt ttcactctga ggttctacac
     8101 cgccaacagc gcaaaataca gcaataagcc catcaataac acgcaatgaa cgttcaacct
     8161 caaccgtaaa atcaacgtgt ccgggggtat caataatatt tatgttatga tcgttccatt
     8221 gacatgatat ggaagctgaa gaaatggtaa taccacgctc ctgctcctgc ttcataaaat
     8281 ccattacagc ctgcccatca tgaacctcgc ccaattttct ggtaacaccc gtaaaatata
     8341 aaatacgctc ggtgacggtt gttttacctg catctatatg agcagcaatt ccaatatttc
     8401 taatattacg caacggcata ttttactcct tataaactat aaaatatctg tcagcaaaat
     8461 aattttaagc tgaccatgaa aaccagtagt aaataaattg atagcttttt atttaaccag
     8521 aggaagtacc actacactat ggttggtcat ggttataaag atagcaaaca tgtcaacatt
     8581 ttaaaaattt tttttatatt ttttaataaa acttaccatt tttataaaat taccaacctg
     8641 tatggcattt taacaacaat actggtggcg atagttactc ttaatgaacc ttttttaatg
     8701 caataacttc tattttataa aacaacacaa ttttttttaa aatgcgatgg caaccctgta
     8761 tctgcttaat aatatagtca cagtaaccca ctcacccttt tagaatatcc tgatatgaat
     8821 acaaaggttt tgagcgatac agtgcataac cctgtcaata aatttatagc aaaacatttt
     8881 tctgcttgaa atattttagc cgtgtaacta aaaacttatc atttcataac agcagtaaag
     8941 tatacccaac atcacctttt caggagggaa catatatgga taacaacgat aggtcacttt
     9001 ccatctttgg aaatcccatc cccaaacaca cctataataa agcaatgaaa caaaaagcac
     9061 gctttgcaaa aaaatttggc tttaacccca atgatcgcta tgaattgtcg gcactaccca
     9121 atcctatctt aaaaaaatat tgtaatctat acactgtttc acaggaagaa ggttctgaaa
     9181 tagcaaaatc aaaatctatt attattggta ccatacgcat gggatatggt cattatcgta
     9241 ttgctatggc aattgcttca gcagctcatt caatggggat tgtcccatac tggtttgatc
     9301 ttttatcatt tgatacagcc ggtgcaaaaa tcatcaaaca tatggataac ctgtattcat
     9361 ttggttcacg cttgtcacag caattttatc tttttaacaa gttatactgg gagcatctta
     9421 ctgccattgg atttaaaaag ctaacatata atgctactga ccaggcaatg actcagctat
     9481 ttgccaatat ataccaaaat ttgccgcaat ctgtaccatt tgttgcaacg catgcatggg
     9541 catcgcaggc tgctgtccat gccggtacaa aaaaagtcat taatatgatc cccgataact
     9601 ggcctcttgc acttcatctt tccgaaggtg ccatccatac agtacaaact ccatcagcat
     9661 actttggtta tcgcaccctc aaaaatatgg gtaaaaaaaa ccaaatactc aatccaatgc
     9721 caaaagatga tatttattac accggtcact acgttgatca tgaactggtt gccaatgttg
     9781 aaaaggattg caatgctcgt ttaaccaggc ttaaaaataa aaagccgcga cgatttttat
     9841 taagcattgg aggggctggc gctcagcaaa agttatgcat ggatattatc atgcactgca
     9901 tcccacttct tgaaaatgaa aaattaacac tcatcatcaa caccggtgat cacaaagcta
     9961 tttttgatat gatacttaat accggattta gtcccagagt tccaaagact tcctatacac
    10021 aatgggcgga aactgaaaaa ctggtcaaca ctttaaccac aagggatgta cccggattgc
    10081 acattgtata taatgaagat attttttcag ccgtttatgc caccaatata ctcatgagag
    10141 tatgcgattg tcttataacc aagccaagtg agctggcatt ttatccagtt cccaagctgt
    10201 ttgttgccca tgtaggtggg catgaaatgt ggggagcaat acgaggcgct gagattggcg
    10261 atagtactgt ggaatgtgaa accgtacagc atacattgca agcaatggat ttattaatct
    10321 atgatgatga tttactgaca ctgtattgca ataatattat caaagcaaaa agtattggta
    10381 tttacaatgg cgcttacgag gtaatcaaat tggcttttgg aaaataggca atataataaa
    10441 aatagagtac agttgcagga ttgtaaacta atcctgcaac tgttatggta attacatacc
    10501 gggaatagag gtaaatactc cccgggggtc tacatcattt cgtattattt ccagaatttc
    10561 ttttgatgga acttcagtta ccggtatgtc acctgctggt ttaacaagct caaatccaca
    10621 tgcaagctga cataaatcaa aagatacacc aggatgcaat gattttacac gcattcgctt
    10681 ggtttctggt tcaaaatcaa acactgcagc ctgcgtaatt actgttacag gacctttgcc
    10741 cagtaaacct gctttctgcc tgctatcacc accgtcaata aatcctggcg aagtaataaa
    10801 atcaaccctt ggaacaaact tttgaggtga ctgcaatcca acaaggatca tgttttcagc
    10861 aagcgaagaa agatcattat tgccgcctga acctgttaat ctatgatcag gttttaaaaa
    10921 atctttccca atcatgtgag tgttaacatt cccatacata tctatctgtg caaagcctaa
    10981 aaatgccttg tcaacataac cattataaat ctgaccaaat gaatacgaca tctccaatat
    11041 taacggtgat ttgcgccatg ttgcaggacc acccaccgac catggcatgc caccttccat
    11101 ggggtcctga gcacctgatt cataaacaag tactatattg ggagcatgag ttttgcgagc
    11161 cagaataccc gctaccattg gtaatccagt tccaatatac acaacttcat gatccttaat
    11221 ctcttttgct aatacatatg ctaacaattc cagcggattc attggtttat ctgatctatt
    11281 acccataatt cctccttatg ttcaaatagt aatagtttat agttattata cattttaata
    11341 aaatttcatc ttggtgatat cttctggcca atcaccctga aattcttcat aattgaaatc
    11401 aacaccttca tcttcattat catattcttc ggctttggtt aatcttcgtg attcagcaat
    11461 ccactttgat ccacctaact tttcaatgaa ttcgtattga tctttaacgc cgaataccca
    11521 gtaatcaaga aatttctgga aattttcatc tgacttcatt ccaaacctga aagccatttc
    11581 ccaccactgt cgtgaccagt aataacatcc aggcatctgc ccggggacag caccatacgg
    11641 aagttcaacc actgcatcaa catacatagc aggaatagat acgcctttat tattattcct
    11701 gatatcactt tctggaacta tctcttcagc ggtaattata agcttgcgag tagcagctgc
    11761 cattgcaatg tcgtttattg ccggaccaaa aaaccgtgca ttcccttttt tatctgccgc
    11821 atgtacatgt ataatagata catcagggta tattgcggga atatacgcaa cagggtcagg
    11881 atcactgcgc attggatttt gcattacctt tacataggga ttatatttta tcatatcaga
    11941 gcctaaaagc tgcttgctga aaacgcccgg caatcctaac tgcgctgctt taaacccttg
    12001 agccattgtg ccatgactcc attcagataa aattgttacc ttacctgcct ttaactgccg
    12061 tgtgtaattt ctgtcaatcc ccctcatctc agcgccaagg tacgaattgt gcgaatatcg
    12121 cgcgcatcct gcattaatca tgtacgaatg attggtattg ggtgaactac aaaactgcaa
    12181 attcttcttt tgctgtcgta taacctcaaa atatgattgg attgaggttc gtacataaca
    12241 aaaaccacca tcagtcatga tatcattatc atgtacatac tctgcaacag cctctttcag
    12301 tgacatccgc ttatctttac gtgatcgatc cttgcttaga tgccactttc gagcatgctc
    12361 aaaatctatc aacctctcaa gaacagaggt atctgtaatt ttgagatttt taactgcttc
    12421 ttcattaaag ctgaattttc ccatttacat cctcctgcaa atataaagta tttcaaaaat
    12481 ccattatata tcaataatta taacatacaa ttttatatat tacaaaattt tctattcatt
    12541 aaataattat atgttattca ttattgttat ttactatata ttattttgta cttattattt
    12601 tatataattt ttgtcaatag ttttgtgcat aaaatattga tatataattg taatatgtat
    12661 gtcatagtat aatttataac atcaggaatt tatgttatct tttattatat gtaattatca
    12721 gtatatttgt ttatatatat ttatatatct atatgtttat atatattcat cattttatga
    12781 tactatcatt ctttaattct ttccctgcgt gaaagtattg tatcggggtc tatttcacca
    12841 acatgctcta caatatagtc cggctggtat gggaacctat ctaccattgc cagtgttgta
    12901 acacccgaca gcacaagcac acatgtcata ccagcctcag atccgcctaa aatatcagta
    12961 tccattctat ctccaatcat gaaacagttt gctgagtgaa catttaactt tttacgtgcc
    13021 cagtacatca ttgcaggatt aggcttccct aaaaagtatg gcgttacagc agttactttt
    13081 tctactggtg caacaagagc tccacatgcc ggtacaggac ccctgtcagt tggcccggtc
    13141 aaatccggat tggttgcaat aaactttgct ccctcctgaa taagataggt tgcttcaata
    13201 atacgggaat aatcatattc ttctgtttcg ccaataataa cataatcagg gtttttatac
    13261 gaaatgacaa taccattttt ttccaattcc tgtattaatc ctctgccacc aataacatat
    13321 gctgagcagc caggcttttg atataccaga aagcttgctg tagccatagc tgaggtatag
    13381 aatcgtgatt catcaacatc aagacccaat gtaaaaaatc tttcagataa ctctttaggt
    13441 gtataatatg aattattggt gaggaataaa aacggaaaat tacctttttg aagattgcca
    13501 ataaaatctt ttgctcctga aattaaggaa ttacctcgta caataacacc gtcaatatca
    13561 attaaaaacg acttttgata acctctatct gtcttcatat agtaccttaa cgtaatacct
    13621 ttaaaaattg ctgaccaatt tcaaatggta tgggcttacc atctgcatag tatcccatta
    13681 cttcctgttt atcgccatgg aaatcactgc cacctgaaat aagcaaagaa tatttttttg
    13741 caattgctaa aaattgctcc acatcctcaa ggctatgcat tgctgaatat acctctatcc
    13801 cctgaatacc cccatcaaat aaatctttta tcaggctttc gccttcagcc catgaaccaa
    13861 aacctaacga aatgggatgc gcaacaatag ggatgccacc actatctttt ataagttgta
    13921 ttgctttata aaatgttatc ttatcttttt taacataacc aggctttcct ttcaccatga
    13981 aattaacaaa aacatctttt acatctgaag cgtatccttt ctttaccagt acacgtgcca
    14041 catgcggtcg tcccggtgag ttgccaacgg cttcctgttc cacctcttta tcagaaatat
    14101 ctatcccatg tacctgtaag tcgtttatta ttttgtatat cctgtctttt cgtaactttt
    14161 gcaggtgcga cagttccctt tttaactctt cattgctatg atcaatcccc atcccaacaa
    14221 gatgaaatga tccatgagga tactcaatgc tgaattcgat ccctggtact aatgtaaacg
    14281 atttttgacg tgcataacaa agcgcctcat caagtccatc aatggtatca tgatcagtta
    14341 ttgcaaggat tttgacacca tgctcaatag cataatcaat aattgcacag ggtttatata
    14401 tgccatcgct tgcagtagtg tgacaatgca gatcaataag ctccattcat catatccttt
    14461 tataggtgta atggtatata atagcacaga agaaaaaaat tgcaatcatt attgtttatc
    14521 acaaatagtt acactgtaaa ctcagagcat ggcagcggag atgttatatg caataaccgt
    14581 ttaagagata gagcattaaa caccgcatac gcattgacat cattatcaaa ataacaatag
    14641 gtatttttat tccatgcaat aatcttgttt gcccactctt caagctcccg catggtgtat
    14701 tctgatgcat ataatttttt tgaaccatgc aaacgtatat agacaaaatt ggctgtaacc
    14761 acctcatgat aagggtatcg cgaggcagta tctgcaatgc accatgcaat attatgctgc
    14821 tgtaataatg aaaaacaact atcgcacaac catgaactgt ggcgtgcttc aatagcatag
    14881 cgatagttat ggggaagaag caatagaaaa tcaccaacaa gttgttcatc aaacttcagc
    14941 gatggtggta attgtataac aataacaccc aatttatccc tcaacaatga tgctgaagta
    15001 acacagcgct gtattgcatc gtcaacatgc aaaagccttt ttatatgagt aatatatcgc
    15061 gacaatttaa ggctatagca aaattcattg ggcaccctgt tataccagct ggtataggtt
    15121 gcatccttaa attgccgata gaaggtggca ttgatctcaa cagtggtaaa gatggatgca
    15181 taatattgta gccacctgta ttgtggaaca gtttcaggat aaaacaatcc cttccagtgc
    15241 ttatagttcc accccgagca accgatgtaa tattgtttgt tctctgttat cattgcgcaa
    15301 ttgcccttta aaaaacatca aactattgat actataatct ataaagcaaa aatagtgtat
    15361 acaatgtcat ggattattca acaaaattcc atacaattcc cccccctttc gttcaaatgg
    15421 gggaataagg ggggttatca gggctctgac cccatttttt atgtcactcc tacaggatta
    15481 aactgcaaaa actgttatga agaacacaaa cgtacttttt gctaataaac tcctccacac
    15541 aatttccacc ttttataaag gggggaatac agagaggggt atattattat cacgataata
    15601 aatagttgtg cacggaggca cggcgtatgg tgaaaaactg tcattctgag cgaaggatct
    15661 tgcccttttt gcaaaagtga aataatagat tcttcaggac tgcgaccttc agaatgacag
    15721 tagtatccaa tgacattcta aatgcagcga agaatctctc tgttttgtta aaacatagat
    15781 tcttcagtcg ctggcgctcc ctcagaatga cagtggtatc caatgacatt ctcattcgaa
    15841 gctacgaaac atagtgacag taaaatctgt tatcggcagc ttcgttgcac gcaggaaata
    15901 ttcaatacaa caatacattg tcacaggcaa caggtacctt accagtaact atcgcacatg
    15961 ctatagtact ataatggctt atatcaatac tggcactata atgtcttgac aaaatggata
    16021 tactgctcat tacagtatgt aaaatcttta tacattgcga tactgatatg gaaattccac
    16081 cggcaacaaa ttttattgaa gagattatca acgaagacct taaaaataat gtctgcacag
    16141 gtgttattac acgcttccca ccagagccta atggctattt gcacattgga catgcaaaat
    16201 caatatgcct taactttggt attgctataa agtacaatgg cagatgcaac ctgcgtcttg
    16261 atgataccaa ccccgagaag gaagatgttg aatttgttga atctattaag catgatattc
    16321 agtggttggg atttaactgg tatggcgagg tgcgccatgc ttcagattat tttcaacagc
    16381 tgtacctgtg ggcaatagtt ttaataaaaa ataataaagc gtatgtttgt gatatgagtg
    16441 ccgaagagat tgcagccaca cggggaacac cttcgcagcc gggaaaagaa agcccttacc
    16501 gcaatcgcag tgttgaggaa aatcttgatt tatttgagcg catgaaaaat ggtgaatttc
    16561 cagatggatc aaagacgctg cgcgcaaaga tagatatgtc ttcacccaac atgcacatgc
    16621 gcgacccggt gatgtaccgt ataaaacacg caacccatca tcgcaccggt gatacatggt
    16681 gcatataccc cacgtatgac tgggctcatg ggcaatcgga ttacattgaa ggcatcactc
    16741 attcaatatg tacattagaa tttgaagttc atcgtccatt atatgattgg tttttagatc
    16801 agatagcatc cggtcagcgt ccccgacaga ttgagtttgc acgtcttaat ttaagctata
    16861 ctgtcatgag caagcgtatg ttgctaaaat tagtacagca agggtacgtt tcaggatggg
    16921 atgatccccg catgccaacc attgctgggt tgcgccggcg gggttatacg cctgattcta
    16981 taaaaatttt tgcaaggaca gttggtgttg caaagaaaga tagcatggtt gatatagccc
    17041 ttttagaaca ctgtatccgt gaagacctta atacaaaagc aaaccgtgta atgtgtgtgc
    17101 ttaatccgtt gaaggtcatt attacaaact atcctgaaaa ccaggttgaa tatgttgaag
    17161 gcatcaataa tcccgaaaac caggttgcag gtacacgaaa aattccattt tcacggatta
    17221 tctatattga gcgcgatgat tttatggaaa atcctccaaa gaaatatttt agattatcac
    17281 cgggaagcga agttcgcttg cgatacacct attttataca atgtactgac gtcataaagg
    17341 atgccagcgg caatatcatt gaactgcatt gcacctatga cccacaaaca aaggatggct
    17401 atgctcccga tggcaggaag gtaaaaggaa ccatacactg ggtaagcgct caccattcac
    17461 tgccggttga agtgcgcctg tacgatagat tgtttaccag agaggatcct tcacaggttg
    17521 atgatggcag cgactttact gattatatta atcctgattc attgaagact gttcatggat
    17581 ttatagaacc atcggttgct aatgctaaag catttaccaa ttttcagttt gaacgcctgg
    17641 gttattttac cgttgaccct gattcacatg ataacaatgt aatatttagc agaacagtta
    17701 cattaaagga tacatgggca aaaatagtac aaaaagaagg gtgattgttt caaaagtatt
    17761 gacaaaattt ttatcagaat aaacattgtg ctataaaggt gttatggtgt tgttttgccc
    17821 ggtggtgtaa ttggcaacac atctgactct ggatcagaga agtccaggtt cgagccctgg
    17881 ccgggcagat gtccattata aagatacttc aattataagt catataagtt acaatttccc
    17941 cagcatatca attgccatgt atactgttgc tcttattatc ttcattatta catattcagg
    18001 gataatggta acccgcctgc caggcattaa cattgacagg ccatcagcag cttattttgg
    18061 agcaattgca atgattgctg caggtgtttt atcttttgaa gaggcaattg ccgctataga
    18121 tttcaataca atagcactgc ttttaggaat gatgattgtt attgcaactc ttgaactgga
    18181 tggatttttt gcttttgttg ctcataaaac aattgctttt tccaaaacgc catcacaatt
    18241 attgtggata ataacattta ctaccggtat tgccagtgca tttctggtta atgacgctgt
    18301 tgttttaatg tttacccctg ttattatctc aatatgccgc catgcacaat tagatcctaa
    18361 accttattta atagctgaaa ttttagcatc taacattgga agttcaatga ccattaccgg
    18421 taacccgcaa aatatgctga ttggtattta ttcaaagatt ccctatatat attttatact
    18481 gcatctgttc cctattgcta tactcggcat gataattatt attattgtta ttaaacggat
    18541 atattcgcaa acctttgaag caccatcact attacattac gatgaaaatg aagaaaatga
    18601 atatgattac aaccttagtt ctatgatctt ttctgtacca atttttttga ttatgactat
    18661 acttttcctt tttcatcata tgcttcattt gtcaatacct cttattgctc tgattggaag
    18721 ttcttttatt cttttattag ggaaaataaa gccttcaaaa attataaaag aaattgattg
    18781 ggtgttactg ttattttttg cttcactatt tattgtcgta aaaggtgctg aaaaagcagg
    18841 gatattgaat atagtgatgc attactcaca gcttaataat actttttata gtactattgt
    18901 attacatgga ataagtctta tctcttccca gataatcagc aatgtgccct ttgtcattct
    18961 tctcgttcca atactgaaac aaaacaacag caaactgcta tggctggcat tggcttcatc
    19021 atcaaccctg gcaggtaatg ccacaataat tggtgccatg gctaatctta ttgttctgga
    19081 atcagcagca agagcaggta tcaaaataag ctttatagaa tttttaaaac ctggattaat
    19141 ggttacaata ataaccttgt taatgtcaat tgtgctatta tatcttcaac tgaactttca
    19201 gttgctataa atttcatagt atcaactacc acgtttatat catacaggaa aatcaaaaca
    19261 aaattgtaaa tacccgtgtg attacaaaat atttgataag ctgcttttta tcttttgctt
    19321 tcaagcatat aaaaaaatga aaaaaacact attgatagct acctataaat atatgcaaag
    19381 gtaagtggta cataattatt ttaattattt gttgacatct ttcaataaat aaattattct
    19441 tatgaataat tatacaataa tttgttatta cctttcacaa cacattattg tatatcagga
    19501 tacactatta atattttaat tgatgagtaa ttgcatgcaa attgatgttc ataaagaagg
    19561 tccactatta atacttacct taaacggacg ctatgatatc tcaaagattc ataattttga
    19621 aactatatac cgacaaaatt tagaagataa tccaaaaatc attgcactta atttacggga
    19681 tctaaaatat attgactcat ccggtattgg ttcattggta cgctgtatga accttgcatt
    19741 gaaaaacaat attgaattta tttgttatga catcaatgaa aatgttgaaa gcatttttaa
    19801 aatagcaaaa cttgatcaat ttatatccat attaacccat gacgagttta taaaaaaata
    19861 tattaaaaag caataagttc ttttagtgtg cctttttctt ctaatagttg tattagtgca
    19921 tcaactataa gcgggtcaaa ttgcgtgcct ttattttttt ccaactcttg tttagcttca
    19981 tggagcgata gtccctttct atatggtctg tcgctcaaca tggcatcaag ggcatcggca
    20041 acagcaatga tcttagcttc aacagggatt ttattgcctg acaattcata atatccctgc
    20101 ccatcaattc gttcatgatg gtacatcaca attgttgcaa tatcctttaa ataatcaagc
    20161 gttgataata ttttcatgcc gcgtatggga tgactttgta aaagcaactt ttccctatca
    20221 gttaatttcc cattcttctc gataatttca attggtatgc caatttttcc aatatcatgc
    20281 gcagcggcag catgattgag caatcgcatc ttttcttttg gtaagccaag atgtttacca
    20341 attgcaagcg agtactgtgc cacccgtgca gtatgaccgc tggtataggg atctttatct
    20401 tccatggcgc ttatgagcac actaatgatt gcgaatacct tttcatctaa ttgtttatca
    20461 agtgatactt ctggattatt tgatccggct aacaatttct gcatatattt taaaatacta
    20521 tcgaaaccat tattatctat ggaaaataaa taggtataat tgcccttttc atactgtttc
    20581 agaaccttca gcatacttcg agcaacaccg taatattgtt tatgcaaaac ttttgctata
    20641 ataataaatg atatgttgaa aataacaata ataaccgcaa tgaagaatac acttttaagc
    20701 aataatgaca gatgatactg atttaaaata aaatgattaa ataacaggaa aagaacagtt
    20761 atagtaacta tcgcagacag taatatgcaa tagagtaaca ctgttacctt tttcattttc
    20821 atagtagtat attgtaaaaa acttgcaata gccatacatt acacctcatt actcaccata
    20881 cgtaaaataa tcgtttttga tgaaataaac caaaaatata atatttttta taagcaggtt
    20941 attaaaatta atattgacga tataatgcag acagttacat ctgtttatat caacattgca
    21001 tgcaatacac aaaattaacc atacttaaaa aaggtagcaa aaatgtcaag gatactttca
    21061 ggaatacaac catcgggtac attgcatata ggtaattatt ttgccatgat gaaacccatg
    21121 atcgaattgc aatataacaa tgaacttttc tgctttattg ttaattacca tgctttaacc
    21181 agcattcacg atccccgtct gctggcacaa aatacagtag atgctgctat ggattttatt
    21241 gcactggggc tggaccctga taaagcacat ttctgggtac aggccgatat ccctgaggtt
    21301 accgaattat catggatact atcgtgccac accagcatgg ggcttcttga acgaagtcac
    21361 agctataagg ataagattgc aaaagggatt atccccaatg ccggactttt tacctatcct
    21421 gtactgatgg ctgctgatat tcttttattt aatgcagagt tagtcccggt tggcaaggat
    21481 caaaaacaac accttgagat aacacgagat attgcacagc actttaatca gatgtatggt
    21541 gaaatctttg ttatacccga agcaatgatt cctgaggata ttgctgtaat accgggtatt
    21601 gatggacaga agatgtcaaa aagctacaac aataccatca atatatttga agatgaagcc
    21661 agccttaaaa agaaggtaat gcgaatagtt accgactcgc agccagtaga agcacctaaa
    21721 aatcctgata gctgtaatct atttgctttg tacaggctat ttgctgaacc tgaaaaaaca
    21781 caaatactta aagaccgata cttaaagggt ggcataggat atggtgaagt aaaaaaagag
    21841 ctttttgcaa tgatatggga atattttgca ccatttagag ctaaacgaaa agcgcttgaa
    21901 aataacaaac atgacatagc atccatacta aaaaaagggg cagaaaaaac ccggccactg
    21961 gctatagaaa ccattcaacg ggttaaagaa gccacaggat taaaatattt aaaataaaaa
    22021 tattaatccc ttatatgcta ttatttttca ctctttttta taactacaaa tttatggatc
    22081 cctccacgct ttaccagtaa aagcagcgtc tctttctttt gatagtgtgc taatagctca
    22141 ttatagcgtg atacattttc taccggcgtg ttttcaatct ctttgataat gtcaccaacc
    22201 atcatccccg cctgatatgc aggcccgtct tcactaacct ccatgactat aacacctgtt
    22261 gtatcggtta tcctgaattt atatgcattt tcctcggtta tatcgccaac acgcagccca
    22321 attatgtcaa gttctactgc tttttcttcc atagcttgct gttgagggag ttctgcaatg
    22381 atgacatcaa catccatctc tttgcctgct ctaaagattt taacttttgc agtagagcca
    22441 ggctttaaag cggcaacatt acggcgtaat tgatttatat ccgaaactgt tgtgccattg
    22501 acagaaacaa taatatcgcc aatttcaaga ttagcgtttt tcgcaggtga attgttcata
    22561 acatcggcaa cgacaacccc ttgcttaccg tcatatttaa aatttttagc aatttcagga
    22621 gtaatgtttt gaatatacac acccaaccag cctcgcgtta ccttaccttt ttgtatcagt
    22681 tcgtctacaa cctgctttac catgtttgaa ggtatggcaa accctatacc catataaccg
    22741 ccatttcggc taaatattgc tgtatttata cctacgagtt ttcctgtaac attaataagg
    22801 gccccgccag aatttcccgg atttattgcc gcatcagttt gaataaaata ttcataatca
    22861 gcaagcccaa aaccttctct gcctgttgcg ctgataattc ccatcgttac cgtatgccca
    22921 aatccaaaag ggtttcctat agctatggca atctctccaa cctttaatgt gtctgaatcg
    22981 gccatttcag caacaggaat gtctttggga acatctccct gtatttttat tactgcaatg
    23041 tcagtagcag ggtcagagcc tatgagctca catgggaatg tacgcttatc aaataaagta
    23101 acggttattg attttgtgtt ttttacaaca tgattattgg ttactatata tcctcgttta
    23161 tcaacaataa cacctgaacc aacagcttta gcctcttttg gcataggaaa cggcattggc
    23221 atctttctgg catcacgcgg tgaacccatt tttttctcgg cagatatact cacaacagta
    23281 ggaattgctt tattggttat ggtcaccaat tgctcattga atgtatttaa atcagcaatc
    23341 gatgttcctt gctgtgtttt tttgattgtg ttttttgcat caacagtaac tatcgcacac
    23401 aaaataatga cacaaactgt tataccatga aatttcattt tcataatgca ttccttgtaa
    23461 tgaaaaaaat atttttattg catttaaaag tatatgtatt caattttatt aacactgcat
    23521 gataacgtta taccattgtt cggtgcagta ttaatagcaa taactattgt aataccttta
    23581 tttcttataa tcatactatc attgttgtca agtcatttag ctttgaggtt ttaatttaat
    23641 taatcgttat gatgagcacc attaatacca ccagtgtttt atttttttct atatcgatat
    23701 aatgctatag tgtataaaga agatcacgat gggtggctta tgacctcgcc aaaaagacaa
    23761 aactatctgg cgatagttat cctgaaatcg tgtttcagta tttaacgtta cattcctgga
    23821 ttctgagtca ggtccgaaat gacaatgtgt tgagtgttat tctgagcgtt agcaaagaat
    23881 ctcattagtg tgaggagatt ttctttgaca ctgtgtgcca tcagaatgat aatgagtgaa
    23941 gattattttt cagttgcgtt ttttctttaa aatgatacta tcgtatatta ttattttcaa
    24001 cgaatctcag agaaaattta ttgtaaataa tatcatggat tagcgaatga aaaagattta
    24061 ttcaccgatg gttatagtag caatgtttgg tgcgcagtta ttactgtcat gctcacatcc
    24121 acaacgctat agtctgtcac aaatggctat gggtacaata gtaaccatca ctgttgacgc
    24181 tccatcgcat gaaaaagcac acaatgcaat acaagcagca tttgacaaaa tcaagcagat
    24241 tgaagcgttg atgagccttt acaagcagga cagtgatgtg tcaaaaataa atgcaaatag
    24301 caaaactcat gtaagcaaag acacctttac cgtcattgaa caatccctgg aactatcgca
    24361 caaaacaaat gggctttttg acataacctt tgcatcaata ggcaaattat ggaacataca
    24421 atcgccaaac ttcattcctc catcaccaca aaccatcaat acctataagc cgtgtatagg
    24481 atatcaacgt attcatctcc atccggaaaa tcttgaggtc atcaaagaaa aaccctgtat
    24541 acagattggt cttggcggca tagcaaaagg atatgcaata gaccatgcaa tagtaacatt
    24601 gaaaaatatg ggtatcaaag aagctattgt tgaagctggt ggtgatctta aggttattgg
    24661 taacaattac ggtcatggct acaccattgc aataaagcac ccgcgtaaag aaggaatttg
    24721 cggaaccgta ctgctgttca atggacagtc cattactaca agcggcgact acgaacgctt
    24781 tgttatttac aagggtaaac gctaccacca tatactcaat cctaaaacag ggtatcctgc
    24841 cacaagtgat gtcatctctg tttcggtgat tgccagcaat gctactgtat gcgatggata
    24901 tgcaactgct ttatttataa tggggtcaga gcaagcaatg tcgtttttgt tgcacaatgc
    24961 atcgttacaa ctccaatgta ttattattga ctcaacaatg aaaatatatg tatcaaaaag
    25021 tttggtgccc tgcacttcgt tcaaaggata tactgtggtt cctttttagt ttattatttt
    25081 tggcgatagt tactgaccat aaatcaagtt gctaaaaaat ttatttttcc caatgacaaa
    25141 atagttgcaa ttcttaattg tttatgattg attaacagtg tggaaatggt aaaacttttt
    25201 aatgaggatg actatggaac tttctgaata tgccgggcaa tcaacaagaa tcaatgactt
    25261 ctatattcat gtatctgatg gaatacaact ctattgcata gattttattc caaaacatga
    25321 caatgtatca aaaccaataa ttttttttgt tgcaggatgg ctttcccata tatccggctg
    25381 gaaagatgtt ttacgttcat tgaccgatga ataccatgta atatatattg aaaccaggga
    25441 aaaaatttct tctaaaatac ctgatggcaa atcatgcaca gatttttctt tcaacgtcat
    25501 gagaatggtt cttgatcttc atgaagtaat acaacagata atacctaaaa aaagaacatt
    25561 cgttatggca ggcagttcac tgggttcttc tgtaatttta gaatatttag ccatgcgaac
    25621 acgtgaacct gaatgtgcac ttctcattgg gccaatccct gtattcagat ttcctcccat
    25681 attaggagat atcgttccca atttgccccc atcatggtat aacgtgttaa aaaaattagt
    25741 tatatggtat ttaaaaactt tcagattaaa taccagaaaa gaaaaagaac agcttatcaa
    25801 atataccaat accatcaatg ctgctgatcc ttataagctc aaacccaatg cactatcatt
    25861 aaaaaattat agtctatggg ataaacttgc ttttattgaa acgccatgtt atatatttgg
    25921 tgcaaaaaca gacacattgc atgctactga agacataaaa cgattgctgc aggaactaaa
    25981 tcgtgctgat tacatagaat gtgagagcaa taaagaaacc cacagtgcaa ttgttgctct
    26041 atatttcata aatatcctta aaaacaagct gtataaaaaa ataaaagcca aaaagcctta
    26101 actttacttg atttttttat atattcattt tatattgaat acaaataacg caaaaaagga
    26161 attgagatgt cattatgcaa agttgacata gttactattg ttctggatga agaaagcaat
    26221 acacctatag ttgttctgca agacaatgaa acgggaaaca ttctgcccat catgattgct
    26281 cctttagaag caagccttat tgccattgag cttgaaggca aaaaaccaat acgtccttta
    26341 acacacgact taattatagc aatgcttcac acatttcatt attctcttga atcagtagta
    26401 attgatgatt taaaagataa catttatttt gcaaagctta atttagttgc tccagatagt
    26461 tctgaaatga tcattgattg tcgccccagt gatgctatgg caattgcatt acgagcaaaa
    26521 gcaccaattt atgtcaaaaa gaaagtattt gcaatagctc agggattaga taatctttca
    26581 cagatagata aagaaacaat gaaagaagtc cttgaagata ttgacataga cgatgttggc
    26641 ggtaaaatca tgtaaatttt tttattagcg gaggatctat gtacaccgaa aaaaacatat
    26701 cagcattgca taatattatc cgcgacctta ctgaaataga ctcccgcctg aacgaatata
    26761 ttgatacaac catgtggata agcgaccccc acggtgcggg agatagattt gtgtcaatat
    26821 taaaaggacg ctttggcctg gtatggcgca tatgttatga agcattaccc aaaacatttt
    26881 caaaagaaaa gatatactat ttaggcagga ttattcgcaa agaacgttat tttgaagatg
    26941 atgtttatag gttagaccgt caggatataa tctcctcact tataagaatc atccaatacc
    27001 gcgtacaaaa tgttcgtgat tttgatgaaa tccgtattaa tataaataaa gaccttaaac
    27061 atgttcttga gaaccttata ttaaactatc ctgttccaga tataatttat gaaaatgaat
    27121 taatagcaga taaaatcatc agttcactgt gtaaaattgt aaaacaggtt atattagggc
    27181 atttaattgt acttggcgat gtatttgaca ggggagatga acctgataaa ataataagaa
    27241 ttcttaatca aaaagacata aagcgatatc ttacattcat ctggggcaat catgatatat
    27301 tatggatggg agcatctgcc ggaaatcaat cacttattgc tgaagcttta cgtatcacta
    27361 ctcgttatga caatcttgct tttcttgaac gtattggtat aaatattgac aaattaaaaa
    27421 atttcgcact atctacatat actggtgaaa ttactggtaa ttttaaagca aaacaagatg
    27481 tatctcgtag aatggaaaag gcattatcca tcatccagtt taaacttgaa gagcaaacta
    27541 tacacaacaa tcctgaattc aatatggaat caaggctatg gcttaataaa ttagctgaga
    27601 tgttaaaaaa caacaatact gatggactta ccgatacaca tttcccaaca attgatttaa
    27661 acgatcccac aaaactttca ccagaagaag aagatattat caatgatcta tcatatcaat
    27721 ttgcaatcag taaaaaaatc cgttatctca tggaattttt atttgaacat ggaaaactat
    27781 atcatatcca taattatata ctcaatattc acgcattgat acccagcaca gaagatggtg
    27841 attttgaaga attctcaggg tacaaaggga aagaattatt agactattta caacatcgga
    27901 ttaaaacaat aggaaagaat tatttagaag gcaaattaca ggatccagga gatttagctc
    27961 tgatgttcta tttatggtgt gggtcaaaat caccattttt tggtaaaaat gcaatgaaaa
    28021 cctttgaacg ttattatctt gttgataaaa caacacataa agaaaaatta ttatattggg
    28081 gtgaaaatct attaaaaaat gaatttatgg ataaaataat gcatgaattt ggcgttgaac
    28141 gaatagtata tggacataca ccaattgata ttcgtaaagg tgagaaaatt gcttcaccag
    28201 atggcagagc tatcaatata gatggtggtt tttcagatgc atacttaaat agagggcatg
    28261 ctcttatcca gacaccttat tcattatatg caataatttt acctactcct gaagaaataa
    28321 tcaatgctcg ccaaaaaaaa gagccagtta atttacaagt tgaaacaata gataactttg
    28381 aaacaccatt acaggtaaag gatacttata ccgggaaact tcttattaag caaaggaatg
    28441 aattgtataa taaattatta gaatataccg atcactattt attggcaact gataatgata
    28501 aaaaggcatt ttattcactt gtataaaatg caacgggcag gtcaacctgc ccgtttttta
    28561 attctatata atatttcatc aataattttt acttactttt ttgcaatctg taaacgcgct
    28621 tttaactttt ttaattctat atcaattgtt aaacgttctg catatctttc tggtcttggc
    28681 aacgctgata tttctttaat ctttgcttct aacagatcag cccttaactc ccctttcatt
    28741 gcggcttctt gagctaaaat gatcagtgaa ttatttttaa tttcgacaaa tccaccatca
    28801 acataaaaat attcagtagc gtcaccatta cgcaatctaa tttcaccagt acctaattca
    28861 gatattaatg gtgcatgatt atacagaaat cccatctcac catcataagc ctgaacaacc
    28921 gcaaaatcaa tctgcccttc atatatctga cgttctggtg tcaatacatt gcaatttaat
    28981 ttcaatgcca taacagtatc ctctaaacga tattagttac cagcaagctt ctttgccttt
    29041 tcaactgctt cttcaattgt cccaaccata taaaaagcct gttcaggtaa atggtcgtat
    29101 tcaccattta tcaatccttt gaagcttttt attgtctctt ccaattttac atatttacct
    29161 gccataccag taaactgttc agcaacaaaa aatggctgtg ataaaaatcg ctcgattttc
    29221 cttgctcgct gtactaacag cttatcttct tcagataact cgtccatacc taatattgct
    29281 ataatatcct gtaaatcttt atatcgctgt aatattcgct ttacttcagt tgcaactgaa
    29341 tagtgttctt caccaactat ttcaggcgat agtattcgtg aagaagattc cagtggatca
    29401 actgctggat aaatcccctt ttccgctatc tgacgcgaaa gaacagtact tgcatcaagg
    29461 tgtataaatg cggttgccgg agcagggtcg gttaagtcgt cagcaggaac atagatagcc
    29521 tgtacagacg taatagaacc acgtttagtg gaagtaattc tttcctgcaa ttcacccatt
    29581 tctgttgcta acgttggctg gtaacctacg gctgaaggca tacgtcctaa caacgctgac
    29641 acctctgaac cagcctgtga aaatctgaat atattatcta taaacaaaag aacatcccta
    29701 cctgacaaat ccctaaaata ctcacacata gtcaaggcac tcaatgctac tcgtaatcgt
    29761 gccccaggcg gttcattcat ctgaccataa acaagacagg ctttgtttat aacaccagat
    29821 tccttcattt ccaaccacag gtcattccct tcacgagttc tttcgcctac accagcaaaa
    29881 actgaataac cgccatgttg catagcaacg ttgttaataa gctccataat aacgacagtt
    29941 tttccaacac cagcaccacc aaataatcca gttttaccgc ccttgatata aggctctatt
    30001 aaatcgacca ctttaatacc agtttcgaaa atctcagctt ttggttccag ctgatcaaat
    30061 tttgggggtt cctggtggat tgaccttctt tccttgacag ttattggtac acctttatca
    30121 actacatcac ccaggaggtt aaataccctc cctaatgttt cttcaccaac tggtactgta
    30181 attggagcac cagtatcaat gattgaagca cctcgtttta caccatcagt tgaacctaat
    30241 gcaacagctc taaccctatt gccacctaaa tgctgagcaa cttcacaaac aagggttttt
    30301 ctttcatcca taacctctac ttctaatttt aatgcattat aaatagcagg taacttgttt
    30361 tcctgaaact cagcatctaa tgtagatcca ataacctgaa caaccttacc tatattctca
    30421 gccatattta actccttaat tcatgaataa aatattatat atcatctacc cttattcaag
    30481 ggctgcagca ccacctacta tttcagagat ttccttcgta atcttagcct gacgagctct
    30541 attataagta atcgttaaat ctcttaccat ttctttagct gcatcagttg cattcttcat
    30601 tgctactcgc ctggcaaagt tttctgagaa tccagtctcc aaaacagcag tataaagctt
    30661 cattttaata tataatggta acaacgaaga aaatatctta taaggattag gttcaaaaat
    30721 atattctaca tggaactctt caacactttc ctgtattgca ccaccttcag gttttaatgg
    30781 taacaaacgt gaaattgtag ctttttgtgt tgatcgagtc ataatcttgg tatatgaaac
    30841 atatacttca tcaacttcac cggtaacaaa aagatttata agttcctcac ctaattttga
    30901 tgcatcatca aatttaaatt tatcttcagg atttaatacc gacttaaaca ttggctcctt
    30961 aataaatctt aaataatttg ttcctttttt ccctattgaa tagactaaaa cttctttgcc
    31021 ttcctcaatt gttagtttac ttttaaaacc aatagtattt tcaataattc gtgtattaaa
    31081 accaccacat agccctctgt taccagtaat cataagtatt aatattctat tgggatccgg
    31141 tctttcctgc attagcgggt caaatacatc ttcaacgccg gtttgtcgca gatgttctat
    31201 aatggtttct aacttttctg aataaggttt cgacattaca attctatcct gcattttttt
    31261 catcttagca gtagaaacca tttccatcgt tctggtaatc ttctgtgtat tcttaacaga
    31321 attaattcgc tttaatattt ctcgttgtgt tgccacagct attcctcttt tttagtttat
    31381 tacgctctta catctgcttt ttttccggct agatagcttt cgacaaaatc acccgctact
    31441 ttcccaaatt tatccggatc tttaagttcg ccagttgatg aaattccatc taataattca
    31501 ggatgcgcat cgcgcaaaaa ttttaaaaat tctttctcaa atcttttcac ttcgggtact
    31561 ggaactttat caagataccc ctgagttcct gcatatataa tagcaacctg ctcagcaaca
    31621 ggcattggct catattgaac ctgttttaat acctcaacaa gtctttgacc tctatcgagc
    31681 tgacgctgtg ttgcaggatc aagctcagtt cccaattgag caaaagcttc aagttcacga
    31741 aactgtgcta aatcaagtcg taatgaacca gcatactttt tcatagcttt aatctgagca
    31801 ttcccgccta ctcgtgatac agatatacca acatcaaccg caggccgcac gccagatgca
    31861 aataaaccag gtacaagata aatttgccca tcggtaattg aaataacgtt ggtaggaata
    31921 tatgctgaaa cctcaccttc ctgtgtttct ataattggta atgcagtcaa agaaccacca
    31981 ccaaggtcat ccgaaagttt tgcagcacgt tcaagaagtc gtgaatggca gtaaaaaatg
    32041 tcacctggat acgcttctcg tcctggaggc cttctaagca acaaagaaag ctgacgataa
    32101 ccattcgcct gttttgaaag gtcatcatat atacaaaggg aatggccatt cttctcatac
    32161 ataaaatatt cacccattgc acaacccgca taaggtgcaa tatattgtag tggtgctggg
    32221 tcagaagcac tggcagtaac aacgatagta taatccatcg caccatactg ctctaatttt
    32281 tcaacaacag atgcaacagt agaagctttc tgcccgatag ccacataaat gcaatatact
    32341 cctttacctt tctgattgat aattgtatca atcgctattg cagttttccc ggtcttacgg
    32401 tcaccaatta tcaattctcg ctgtccgcga cctatcggtg tcattgagtc gatagctttt
    32461 ataccagttt gtaatggttc ttttactggc tgtctatcgg caataccagg agctaaaaat
    32521 tcaacaggcc gcatctttgt tgtattaatt ggtcctttat tatccaatgg tatacccaga
    32581 ggattaacta ctctacctaa cagctctgga ccaacaggta ctgccaaaac cctgttcaat
    32641 ctcttaaccg tatcgccttc ttcaattgaa agatagtcac ctaaaataac agcaccgatt
    32701 gaattctctt ccaggttaaa aaccagacca tatgcaccat tttcaaattc aagcatttca
    32761 ccagccattg cattatctaa accataaatt cgtgcaattc cgtcaccaac ctggataaca
    32821 gtaccaactt cactaacatc caactgtgac ttataccctt caatctcctt cttaataatc
    32881 gattttattt cttcagtttt aatcttcata agccacttca cctctgatct tactttgtat
    32941 taatttttca cgtaacttta ttagatcttt ctttaaggat gcatctataa ttaaatcatc
    33001 tacttgcaca ataattccac ctaaaattga tggtataact tcttcttcta taattatatt
    33061 cttgttaaaa cgatcttcta aactcttttt aattctattt ataatatcag catcaagctt
    33121 tatactgcta attagtttaa ttttctgtcg attattgatt atatcaagta attcaccaaa
    33181 ggcgacatta atatctggta tatcttgttg acgtccatta tcaattagta cctttaaaaa
    33241 gttaaccatg agatcagata atttaccacc aaacacctta tcaacgaatt gtttttttga
    33301 atcattatta attccaggcg cattaaaata acgcaaaaga tcaggatcat tttttaataa
    33361 ttctgcaaga aaacttaact cttcttcaac ttttgctata gcgtttttct catgcgcaat
    33421 ctctaataaa gcacctgcat aaactttcgc tacttcactt gttgccacac cgataaccct
    33481 attaattaaa atttaaggac agttcagtac tactgaactg tccttatttt ttgtaatgcc
    33541 tcttctacaa gggctttctg atcttcaact ttgagattct tagctataat cttcgctgca
    33601 atatccgttg atattgtaac aacctcagat tgaatctctg ccagcgcttt atcttttgca
    33661 agatttatct cccgacgcgc tctttcaata agatctttgg cttcctgatt tgctttttca
    33721 actatggaat ttcttaacct ttccgcatca gccttacctt cagcgatgat acttgcagcc
    33781 tcctcttttg ccttatccat cattgcttta tgctcagcaa atagcctttc ggcctcaaga
    33841 cgggactttt ccgctgattc gatatcacct cgaatcttct cagccctggc atcaagcgcc
    33901 tctacaattg gcttccatgc ggctttccat aatatcaata acaacactat aaaggttatt
    33961 attgtccata acaggaggcc gggttcaacc tttagcaagc cttcttttaa aacttccata
    34021 gcgaactacc tacaataaaa tttacttaac ttcagtttgc tctgcttgag gtttctcaac
    34081 cggcgttgta gcaattggag cttttgttgc aagcataaaa gttacaacga gagcaaaaaa
    34141 agtgaaacct tcgataagag cagcagcaat aatcattgct gttcttaagt caccacttaa
    34201 ttctggctga cgagccatac cctctaaagc accagtagct aatttaccaa taccgagtgc
    34261 cgcaccaaaa actatcaaac cagctccgaa acctgctgcg aggtaagaaa ggcctaaata
    34321 ttccattgac tcctcctcga aaattttgaa tattaaattc taacaattat gttattgcta
    34381 agaaatatat tgattacctc ttaataatgc attgcttaat gtgcatgcat tgaggtacca
    34441 ataaacattg ccgaaagtac tgcaaatata tatgcctgta taaaagcaac caacaactca
    34501 agcaaatata tcatcaatga acctgctact gaacctatgc caacccagta actctgaaac
    34561 atcattacca gataaataaa tattattatt acaacatgtc ctgctgtcat atttgcaaaa
    34621 agtcggactg ttaaagcaaa tggcttaatt aataatccaa taatttcaat gggatacatt
    34681 agcggcaaaa caaacgcagg caccccatgt ggtacaagcc ctacccaata cccaaaaata
    34741 ccctgcttga ccattccaac tcccagcatt ccaattagtg tacaaagcgc caatccaccc
    34801 gtaacagcca ggttgccagt tgcagtagac atacctggaa caagtcctat caaattacaa
    34861 aaaagaataa agaaaaaaac agtaaggaaa taaggcattg cctttttaac ataatgatga
    34921 tcaaaatttg gctccacaac ctcatcatgt ataaatgcaa ttaacacttc ccatagatta
    34981 acccagcggg acctggaacc ataaacagaa ttttttattt tacgggctac aggaataaaa
    35041 ataagtaagc agagcaaagc agctatccac agcattataa cccatctggt gatagtcata
    35101 tcgaaaatac caaaaagttt ttcatgaaaa aaaagattat tgattttacc aatgaaacca
    35161 tcatgaatag ggtgatcagt aagatggtgc atgactatat catacatcga ctcttcttca
    35221 ccgctttttg aagattgaat aataactggt aaactttgta gcagctgaat cataacttta
    35281 catacctcat caactttttt tgttattgta ttgatgcaac gtcattgtta caaccattta
    35341 cgccttttag aaccgctact gttactatca tctgcctgtt gggatcgttt cagcaatcga
    35401 tccaactcgg ataaactttt catcaaattg taaaaaccta ttcccattcc aagtacagta
    35461 cctataacca caaacagagg actgctattg aaatgttgat caagaaaaaa accaccataa
    35521 agaaaaacaa gaatcgtaat tgcaagctgg attcctataa ctgaatatcc aagtatgatt
    35581 cttgtaacat cctttttcac ttacacctca aatatcagcg ttggattcca tacaaacacc
    35641 atagcttttt ttgtcaacaa aataagtctt atttttttta aaaaatatta ttgttatcat
    35701 atgcaatttt atatgtcaaa tataattatc ctatttattt tgatgtaaca ataaaaatta
    35761 gaattacttt ttgatattat ctttttcttt tatatagtcg atacaataaa taattagaat
    35821 gtaatcgctt atgctgaggt ttacatatga aattatctat taagctaatc gaaaataata
    35881 atgaatttat cgcaacctgc cctgaacttg atattagttg ttatgcaaca agcaaacatg
    35941 aagcaataaa aagaatacag aatatattat ttttttatat gcaatcagct caagaattag
    36001 gatttagcgt agaaaatttt gaaacaattg atgttgatgg caaacattat ctccttagat
    36061 atgatatctg tccgacaacc aattcaactc tccattaagt aaaatcaaga aattgtttta
    36121 tacaattaat atacataata tacactgtcc cgattaccga taatacgatg caaaggaata
    36181 ataaatggcg aggagtttac ccatgaccgg cctgcagcca cctatgctgg atggaaatat
    36241 tttgtagcaa tcctcctgaa ctttgattca ggatctaatt attgaaattc caggttaagc
    36301 tcagaatgat gtttataaat aattctattt tcagggaaaa aataattttt ttgacaacat
    36361 gattcctcct tgccatgctt atcttaacac atcctatcca tgttaatcat tgaaattttt
    36421 taacaatata tttttatttt ccctcaagct cagcctcatt gacactaaat ttatagatat
    36481 gcaacatatc ccgtttccat tttgctaaat tatcagctct tttttttcta taaatcatca
    36541 ttgctatttg atcttttact ttgtcaaaag atataatctg cttttcagtt ttacctttct
    36601 gatttgtata ggttgtaaag cgtgtatctt tgtatttttc atattcatca cgtacttctt
    36661 taggggttac gattacatcc ctatcggcaa tataatccat atattcttta gcgaataaca
    36721 tttgctttcg tttttctact tcctttttga attcttcatc attatgcata ttttccttta
    36781 tagctgctct tctcagcaat gctactttaa atgcattctt tgctatatca actttttttg
    36841 tatcttccat cacagattta tgaaacactg gttgatcatg tttaaaatat gaaatctttt
    36901 tttccagatc cccaacggta aaaatagtgt ccccaacttt aaaaataata tctttgggat
    36961 ttttactttt acatttttgt tcgttaaatt ggacatcctg tgcagacatt aaattatcaa
    37021 tataattgtt tgatgctttc ctgagtaaaa tctgttcaag gcgtttagct tgcatctcat
    37081 ctttaattat tttattcaaa ttcttctttg taatgttctg tttctcaaca atttgaatta
    37141 tatatatacc attctctgcc cggattggcg tttctaacaa tccaatatta gattcattga
    37201 tagcctggta tagcgaagat tgtatcatgt ctttaactat atatccaata tccccgccat
    37261 ttttctttga aaagtcatca gaaaactgct ttgccagttc ttcaaatttt tcaccatttt
    37321 ttaatctgcc aattatttct ctggctttat tctcaacatc gccataagcc ttttccagtt
    37381 cggattttgt taaatttaca cgcttattgt taacaatttt aaagtctctt actcgcaaaa
    37441 atatttgttt aatgtggaat gctggttcat tgaaatctgt gtttttcttg atttttttat
    37501 cgtataatgc tttcaacaaa accgattcaa aagccatatc ttctaaaaat ttaagctttt
    37561 cagatgcttg caaattatct ttttgcatct ctttcagagc tatagattca attgccataa
    37621 gctccagttt atctctttgc tgttttttat tttttaaaat tgtttcaatt ggatattgtt
    37681 tatctttcaa ccattgttta aactggcccc gtgtaatagt agtgtcacca tagcgtgcaa
    37741 ggatatcaga attacattta cacccaatat aagcaaatat aataattatt acaaacgatg
    37801 cagcaataag ttttttcatg atataatcct ccggaaataa aatggtaaat taagaaaaga
    37861 atcatgtttg ttaaacaata tcatggaata aaatatatct ttattcattc aattttatat
    37921 tttctgaaaa tctatatggc aaaaaccaat attttttgca attaactttt taaaaaaaat
    37981 tattttaatt gctattgaca gaattgaata tttaattatt gtgctcacat tagctctaga
    38041 ttaagatttt acaataattt tataagtgat agttacctta tgcgtgacaa agctttttat
    38101 aaaaatagat tgttttcgtt gatacaccat gacgctccca ttgattgtaa aacattaatt
    38161 gaatatggat atgatgctat tgaaaaagga aacttcataa aagcattcag attgtttatg
    38221 ttaggttatc gtttaatagg gaataatgct gaaatcctta atggtatggc actttcatta
    38281 tgcgagcttg gtagattaaa attttctaaa agtattttgt tatttgcact aaaaaaattt
    38341 cctgataacg tattattatt aacaaatctt gcaactgtat actttgatga atataattat
    38401 gataaggcaa tcttttatta tacaaaagca atttccattg atcctttttt tattgaagcc
    38461 catataaatc ttgttaatgt ctattgtgag cgtggtgata tctttatggc atacatagcc
    38521 tgccttaacc ttttaaaaaa acatcctgat aattttgaag ctcaagaatt atgtgacgat
    38581 atcattatta atatgggttt gtcactggtt taatcgtttt ttggcagttc accttttaaa
    38641 ctaatcttcc attccccatt ttcttttatc aatttgtgtt taattgtcat gcctatagca
    38701 tttagttttt tatgtttaac gcattgtacg gttacccatg cttcgttggc tgttctggac
    38761 actgcaacaa catcccatat agcattgtta tcatgatgca cccatacata aggatacatt
    38821 gttccggcat ctttctttaa tgtttcaata tattgtattg aatctctatc cagatagcca
    38881 tgcaatttct tttcatcagt agttgcaaga tagttaataa caacattagc tgcatcatca
    38941 acaggagctt tgctgcatga tagcattatt atcaaaagaa acagtaaata tttttttatg
    39001 gtatatgata gatttttcaa atagcatcct ccaaaaatag aattattaac atttatcact
    39061 atgagcttaa tacggaatat caatcatcgt caaatatcct gaaatgaatt ctggatggca
    39121 caccacaaat acattattct gaaaaacaca tcactttttg tcattctgag gaacatagtc
    39181 ccaaagaatc tcttctttca taaaatgatg agattcttca cttcgttcag aatgaaagac
    39241 attatcaaaa cgatgcgatt tttcactgtg ttcatgatga tatggcaact ctgtaataca
    39301 atccattgct ttgactttac tcagcacagg tacttcgttt aaaataatga ttggctttac
    39361 atcaaagaga caattatagc agcaaaactc tttcaccagt tactatcgca cataatatta
    39421 ttcccatgat ttgcgatagg ataaacgttc tttcaaggta tagaagtcac tccccttggg
    39481 aacatcacgc aatcgtgttt taagagtatc ctgatcaatc aactcactga atggcactgc
    39541 cttcagatca aaattgtcag ttactgaaac cattattcca aacatggttt cattgtaaaa
    39601 tttgtacgct ccatagccca gcatcgcacc taataccacg tcaaagctaa taggtggtgc
    39661 cgaacgcgtt tcgtagccaa tctgtttggc aataatttta acctccatgc cagttttttc
    39721 ctgatatcgt ttctttacac gctctgcaag tatctcccca attttagctt ccctatacag
    39781 gacatttcca tgtttatcag tatgctttgg tttgtactca tcagaaagct tatcaacaat
    39841 accttctgaa acagctataa ccccataggg acgtctgttg ttttcgcgtg ccagtataac
    39901 attggtaatt ttatcaacta acacttctaa atcaagcata tcctcttcaa tatcttcagc
    39961 agcaatcatc attattgcct cagccgctat tcctgctgca taggtaatcc accctgcttt
    40021 gcgccccata agctcaacaa taaaaaaact atctgttgac tgagcatctg ccttgagatt
    40081 aagtattgat ttttgtgctg tatcgacagc cgtccaataa ccaaaagtcc atggaatacc
    40141 atagtaatca ttatcaattg ttttggggat gtggataaca gggaacccta acaggtttag
    40201 gtaatttgca gtttttaatg tatcatcacc tccaatcgat atcaaagccc caatttttag
    40261 atattcaaat gcatctaata tgttgttaag cttttttact ttttcagaat ctttgatgtc
    40321 ctgaattgtt tttatatctt ttcctggatt tgctcgtgat gtttttagat atattccacg
    40381 ctcattgcgg acatgagtta ttgaatagtc tatctcttca aaatggacac cctttttgat
    40441 tttaggatag ttaatattaa agtcctgtaa aaattcatat cccctgtaaa agccaataac
    40501 gggtatacca ttgtctaaaa agtttaaagc aacagatgat ataacggtat ttgcactggg
    40561 cgcaggacca ccagaaaaaa gcatcccaac ccttttgtaa gcaggccgta atttcatata
    40621 caatcctcgt tttttaaaat tctataataa gcaatcccgg ttaaataata cttttatata
    40681 tgttttatag caattaaaat ttttattgtt ttaagataac cagtgcatca actgccaccg
    40741 gtttatcacc attaatgata attggattta tatcaatctc agctatttct tcatatgtat
    40801 tgataagttc acttaaacca actaaagctc tggcaagcat ttctttatca acagcaggcg
    40861 aacctctaaa cggtcctaat agtttttttg tcctcaattc atccatcatt tcatatgcgt
    40921 ctatactggt tatgggagca accctgaatg aaacatcctg caatgcctcg gtgaatacac
    40981 cacccaaccc aaacataaca catggaccaa actgtggatc tttaatagaa cctaatacaa
    41041 attccctgtt atcaccgtca agccactcct gtaccagaac accatcaagc ggtatacctt
    41101 taccagtaat ttcatcatag gcattggcta catcttcttt tgtcttcagt tttaacttta
    41161 ccattcctaa ttcagtttta tgagttagcg tatcagaaca tcctttcaaa accacaggga
    41221 aacccaactt ctgtgcataa tcaagagctt cctctttgct ttttgccaga tagttccttg
    41281 aaacaggtat ccctgccatt ttcattaatt gttttgaatc aaactctgat aatgcttttt
    41341 gtcgtttagc tattgcctga tttaaaattt cacgcatata ccatactcct tttggtgacg
    41401 taattataat agttatacat ctttgcaaca cacaacacag cctcttcggg tgtatcataa
    41461 atgacacact taaacctgcc accgcaatca tgaattgttt catcttcaaa agattttgaa
    41521 aatgataccg ttactatagg cttgttgtaa attgccatta attctgttaa tctgtcaaga
    41581 aattcctttt ctaatacgtt taatgatttc ataaaatcat cataaatact tttttccatt
    41641 tttcccagat ttaaaattga ctctgcagca cgcaaagcaa aaacttttgc gccaattatt
    41701 cccaatacta tcactacatc aaatgcatca ttagcaacca tcaattcaac agcatcttta
    41761 aaaatctgaa aatcaggttg cccaaccaga tcaacaggat tacctctgct ccagaacgga
    41821 ggtaaccgtt tatcaagctc ttctacaatt gattgtggca actcaggaat taaaagtcca
    41881 cgttcaacac attcatctgc tgttatcact ccccagccac cacccaatgt aacaatacca
    41941 attctattcc cttttggtaa agggaatgag tcaaaagctg ctgaaaggct caaaagctct
    42001 ttaggtgatt cagctaaaac aattcctgtt tgttgtacaa cacctttaaa tacagaatat
    42061 gaaccagcca gtgctcctgt atgtgatgct gctgctttgc ttcctatttc agttctccct
    42121 gctttcagcg ctattattgg cttttttaat gaagtatttt ttgcaatctc aacaaaactc
    42181 ttgccatcgt caattccttc aatatacatc agtattactt ttgtttcact atcgctttta
    42241 taatattcaa gataatcttc aatctttaat actgcctcat tacctgaacc aacaaattta
    42301 gatattccaa tattctgact tcttgcccat tccaaaatct ggaatcccag attgccgctt
    42361 tgtgcaataa ttgaaattcc gccaggtttt ggccgggtga tagttcctgt agcatacaat
    42421 gagcagtgtg tattgacaat acccatagta ttaggtccaa caatatgcaa tccccgtgat
    42481 tgagctaatg caacaatatc attttctaac attgcccctt ctttgccagt ttcactaaaa
    42541 cctgatgtta tcataacaat attggttata cccttctcta tgcaatcctg taatatgtgc
    42601 ataattgttt tagaaggcgt tgttataaat acaagatcta ccggttctgg tatatccaat
    42661 aatgaaggat atgttttata tccaaaaacc tcaggaacat taggattaac aggataaaac
    42721 cttccattaa attttccatc caaaatattg cttgcaatat atgttcccca tttcaatgga
    42781 acttcagaag caccaacaac agcaactgag cctggattaa acaaactttc aatcatagtt
    42841 tacctcatgg acattaatat ataatttgtt tactatcaat gaatgaatat tatatttgta
    42901 tgtcaacaat tttgttagat atatttcaat agccttttct cttttgctgt gattcccatt
    42961 tccaggctgt tgcaacaatt gaatcaatat gggtatagtg aggttgccat tgcaactgta
    43021 gtttcacttt ttttgatgaa gccaccaaaa ttgcagggtc cccttttctg cgttgacctt
    43081 cctgtacttt aaaatcccta ccggtgacac gccttactga tgcaataacc tcttttacac
    43141 taaaaccttt accattgcct aaattaaaaa cgtcactttg atttgtttca ataagctttt
    43201 tcaatgctaa aacatgtgca tcacccagat cattgacatg gatataatcc cgtatgcatg
    43261 ttccatccgg ggtatcatag tcagttccat aaattgttac agtatatcct ggatcaagca
    43321 cagcacggat taccagcggt attaaatgtg tttcggggat atgactttca ccaatttgcc
    43381 cgctttcatc agctcctgct gcattaaaat agcgtaaact cattgcatgg aatgaatctg
    43441 atgcagttaa atcctttaac acctcttcaa tacaatattt tgtttttcca taggggcttt
    43501 ccggcttcat aggactttct tcaggaatag gaacaacatc tggatttcca tatacagcag
    43561 cagatgaaga gaatataaat tgtttgatgt tggattgcaa cattgccttg aacagggtca
    43621 gcgttttagc aacattattt tcatagtatt ttaatggatt ctgcactgat tcgccaactg
    43681 caataaagga cgcaaaattc ataacacact gtatatcata cttttttaat acaaacatca
    43741 ctttatcaaa atcgccaata tcaccttcaa taaaatcaaa acctgaaaca aaatctttaa
    43801 atccgccgct gagattatca tagacaacag gattaaatcc attctttgcc agtagccgta
    43861 aaacatgact gccaatatac ccggctccac cggttactaa aatgcttttc attacaattc
    43921 ctccaaaata gaattatgca cgcaacgtca tcccgggcta aacccggaat ccagataggt
    43981 aacatcagat tctgaaataa attcaggacg acatcctgca tttgatgatg acaactgcaa
    44041 tccgacttca tctcatagtt ggttatcctg ccaaattatg gataaccgca agccgtccat
    44101 gggtaactcc tccgaaaata gaaacattat actggttaaa cctttttata aaaaatgcct
    44161 ctgttttgca caacccttgt aattttacgt tgtgcttcta aatcatcaag atatttattt
    44221 agttcatcgc tattgatatt aataatctgg gcaatatcat ttaccgtaca tggccttcga
    44281 gctatagtct ctaatattgc agattcaata tcatatcgat aggcagtatg aactttatca
    44341 atatgtgaag atggagaaga tataatgcta acattgggta gctgccagta ttgtataata
    44401 tgctccaatt catccatcgt tgctgctcga atgctggaca aaatacccgg tctatccagc
    44461 gtattaagct gtacctcatc aggatgtata gtcagtattg catgtttcaa tgcatcaaga
    44521 tcattattgt tatcatttat ctctggaata atgaaaattt ccagtataag cttcccttta
    44581 aatatctttt taaattcagt aattccatta ataatggttt gtatattcaa tgaaggatgc
    44641 ggcatgttaa tttttttaaa agtttcctgt gttgcagcat ccagagatgg gataacaaca
    44701 tccgcaccca agagctcttc acgtacctgt ggcatatata acaatgtgcc attggttaat
    44761 acggctgttt taaacgttcc atgctgttct tttaaaaaat caagtatgat gccaatgcca
    44821 gtatgcaatg tgggttcgcc cgatcctgaa aaggtaatat attccgggca gggattttcg
    44881 tttaagtaac gcttcaactc atcaatgaca tcatttattg gtacccattc ttttcgctct
    44941 attgtcagtt tggtggtttt accgcattca caatagatac aattaagcgt acatgtttta
    45001 tgcgggataa gatcaactcc cagcgaaatt cctaaccgcc gtgaaggtac agggccaaaa
    45061 agataggtat acatattaat atcccaatgc catcataacc gatccaataa acaatccaat
    45121 aataagatta ataaatttaa tcaatacaaa atatttcctt gattgtgcta ataacggcaa
    45181 cattgcatgg ccatcctgaa caatggagct tgtaaccagc acagatagcg gcaccaaccc
    45241 tttcgcaaac atcataacaa ataaaagatg gggacccgac tcaggtatta tccctactaa
    45301 tccggcaata atcattacca tccattttgc atgatgaatt gaagcatcaa tatgtaaaat
    45361 attcaccaat acatggataa caaatagcgc cgatactgtc catagaaata ttctgggaag
    45421 atgacgttta aaaacatgtt cccataaatg ctcctgtaaa aaatgctcgg gaactatcgc
    45481 cactataatc attgcaatta ctactattat cggtacagca acctttacaa aaccttcttc
    45541 ttcatggaat acaccggtta ctatcataaa aaatattatt actaatactg acatcaacga
    45601 agcccttgac aatgataggt ttttccacaa tgcagcaatg ttgtgcagcg tataagaaga
    45661 aaaagatact tcattatgta taacaagtcc atcacagcat tgttcactat tactgtgctg
    45721 tttaaaaata atatcaacga tatatcctgc tgctatagca atgattgcaa gccctatatt
    45781 catcagcaat gcagtttttg gaaacattgc aaacataaca aaggcttcgt cgccactggt
    45841 ggcaagcatt gtggcaacaa tagcacctaa tgatattgcc ccatgcgcat acatggctgt
    45901 tgccgcaaaa ccacccagac aaccagggat aattcccagt acaattgcta tacagtattg
    45961 ctgtaatttt tttgatttta aattagtaat ttttccttga gttacaacgt ttatatactc
    46021 aaccagaagc attataacga caacaaacat agtaataaac aatgattctt ttattatatc
    46081 aattatctga tgcatattgt tcctctattt tcctctatat agtaaatagc atagtataaa
    46141 catcgttaca aattttgtat atatcaaatt ttaaaaattt aaatacattg ctatcaagaa
    46201 ttatacctct atagagtttt tatataaaat ggatgttcat gttgccgtgg tattgcgtat
    46261 acataaattc attgcaacaa acagtattgt gctatgctat tgcaatttta gcaacattaa
    46321 aataactagt acaatttttt gctttaaaat tattgacaaa accattatat aactatttaa
    46381 attcatgagc aattaaggta atcatgaatt ttcaatgaat tcattatatt ttttatatat
    46441 accatatttt tgtaatattt ctttatgctc ggtactaatc agaataaagg taacaacgtg
    46501 aaacttcaga gtttaaaaca ttttgttgca tgtctatttc ttttaccagt actgatgctt
    46561 tcatgctcgg atgaaggtat taccataaag ttcatggttg tatcaaccgt taaaggattt
    46621 gatggttact atattgttga cggtgattct gttggatatt ttagtgctac tgaaaatgcc
    46681 tatggcattg caatgtatga gaaagaaata gatgatgtgg agtatcttga ggtcagcgca
    46741 acaactcgtc gggatgcaac atctattgaa atcaaggtat accgtgatga taaaaaggtt
    46801 aaaagcagtc agacaaacat tgaagaccct tttgaaccca caatacttaa tcttgaatat
    46861 tcaattggcg aagaagatga agaaaaaacc tccagctaaa ttaattcaaa ggcaggatgt
    46921 gctatgttaa actttgaaat acatgatgca acacgtgaac tttcaatagc gcgtgcatta
    46981 aaaaaatacg agcccccctt taaggtaatc gatagctacc ggttaattga tgatggatct
    47041 aatcctgaag caaccgtaca gatagaagcc aatggacgat atatccatga agcttccacc
    47101 ggtgacggac cggtagatgc actggcaacc gtattgaaaa aagcactcat accacttttc
    47161 cccgaaatag aaaatgtaaa gcttattgac tatcgtgcca atatactgga tgccaagcgc
    47221 ggtaccagca gcaatgttga agtaaccatt atctttactg atggtgaaca tgtgtggaag
    47281 gtatatgcac tttcagaaaa tatcaatgca gcttcattca gggttttgct tgatggattt
    47341 gaatttgcta tcttaaaaaa aaataaaaaa accatcaaca aatctcaaga gctataactg
    47401 caatatcatc atcaagggtt ttgttttgtg atgttaccac ttcaattatt tttggcactg
    47461 catcatgcag tgcaatttgt tttgttgcaa tgatttcttt agtgagtatg tcaaaccgta
    47521 aatctttttg attattgata tctatttcaa ttagaccgtc agtatataaa aataatctat
    47581 cccctttttt caattcaata ttttttacta aaagtttaac atttttctct atacccagcg
    47641 ggatacctga actatccagc atagcaatag catcacctga taacactaca ggatagggat
    47701 gtccgctccg tgtataggtt agcttaaatc cgccatctaa aagtggggtt attaatccat
    47761 acaatgcagt aaaataatat cccccaagaa taggaaataa tttttcattg agctgatgta
    47821 taaattcatc tggttggata attccattat tccataaatt ttcagtagca tatttcaaaa
    47881 ccgatgaaaa caatgcggca ggaacgccat gacctgttat atcagccatg aatacggcta
    47941 aatcatggtt aatctttttt atagtataaa aatcgcctcc aacaaaatca tacggacgat
    48001 agcaggtaac aatttttatt ccaggatatt ctgggatggg ggtattaatt attgattgct
    48061 gcaccctttt tgcatattct aagtccttgt ttataatatc atttcttttg cgaagttcct
    48121 cttcgctttt acgcaatgcc tcctcctgct ctttcttctc ggtgatatct tctttgatag
    48181 ctacaaaatg agtaacgttg ccatgctcat ccgcaactgg tgcaatgata caatgttccc
    48241 agaaatagct tccatttttt tgtttattat ggaaaattcc atcccatacc tccccacgta
    48301 atattgtttc ccacatattt tgataaaatt cctttggctg atgacctgat tttaaaatac
    48361 gcggattttg gccgatagct tcagtaaaac tatatccggt aatagattca aattttggat
    48421 ttacatattg aatgatcccc ctggtgtcag ttatgacaat tgaagccggg ctttgttcta
    48481 tggctcgtat taaaactgcc tgatcaatgg ctgataatgt tttcttcatg tatagttacc
    48541 ttttgttcct taatcacacg gcattgcacc ctaattcatt atactataaa aaataatctt
    48601 tatgaaaaaa atttattaac agtaactatc aaacaaaaaa aatactttaa tgaattcctt
    48661 attaccattg ttaccctatt gatgtataat attacacact cattgcaata tataaacaat
    48721 atttttcaat gttctgaaag aaaatatctt gacccttttt ctatcattac atttgatagt
    48781 gccatgagaa aaaataaccg ccagcatatt cttgatactg cccgtaaact tttccttgaa
    48841 tatggatata atggcatatc aatacgcaac attgcaaaac gggcaaagct tacaacaggt
    48901 gcaatttatt ttcattttaa aaataaaaaa gagatatata gcactatatg tttagaagct
    48961 atcaacacat tgcacaggct ttttagtgat ggcataaaac tgcgtaaaac tccagcacaa
    49021 aagcttatat caacatacga ctcgtattta aaattttact atgaatttca tgattattac
    49081 aatgtcctta ccgaatacag agccgaatat gacgatgagc ctgaccccta taaagatgaa
    49141 attcagaaac aaaatcttag tatacaaaat ttaatggcag atgtatttaa agagggcatc
    49201 gatcagggtg tgtttaaaga ccttgaccca aagatggtgg cattttttct ttcagcggtt
    49261 gctgaaggta tgttgcaatt taaaaagctg ggtgtaatgg atgccgcagg aatcaccgac
    49321 aacaaattca gggaatttat gaagattata gtcggacagg gcatacaaaa aaaatgaaca
    49381 ccttagcaaa tccactgacg atgtcacctg taacattgct cacctcacat ctaacacctt
    49441 acattgtaca ctgtacattt tgcatattac attgcatacc taacaactca atatctgttt
    49501 ttaaaatggc actttatgaa tgctattgtt tcatcttttt taacaataga tatcgctgca
    49561 actcgattat ggcagcagca acaatgagca tccctgcaaa aacaaggatt gttttcacga
    49621 tgctccatcc ccacagtatt ggtgcactca ccagaagtgt ccccaaaagt acatatggtg
    49681 caccaaatat actcattata ccagcttcta tagctgtggt ggttttaaat tctggatcag
    49741 ctattcttaa aagtaacaaa cctgttgaaa ctgtacctgt caccgtacca tagatcaaca
    49801 gcatcctttc cagatttacg ctccataacc tgcgtccata atagtaacat acaatcgttg
    49861 ttattattcc tgctaaaaca caaatcacta ctattggaac aatatattgc cacaccacta
    49921 cgacctgaat agccataact gttgcaacta acagataatc cactgaaaac ccggtgatac
    49981 gatgctgaat accgggatca ataagataca atccccctaa ttttgtaata ataaatctta
    50041 caatgaaagc tatcaaaagc ccgataaaaa agaaaaaacc ccacatcatt tgccccatct
    50101 cagggcttaa taaactgctt atcccataaa taacgacgta ggtgatcata taaacaacac
    50161 caacaagtga tgcctggaaa gcaagcgcat ctacatttga tgaatgcgtg gttaattcac
    50221 ctgccacctc cttttcagat tgctgagcca taacacctct tcgtaattcc attgataact
    50281 ctttgggagt gtacactgcc ttacctttgc gtatacctaa ataagcaagt ggaacaccta
    50341 taaaaaatga aaacatataa ccagcaacgg cgaaggttaa ccctaatgta gcagaatgag
    50401 catatcccag attttcccac actttcccaa ccgaaagcgc ctgcccggga ccttcttcaa
    50461 atcctatcgg agttaaaaaa ccaaatgaat taaaaagatt atatcctaaa taattcaaca
    50521 ccataacaca caaaccgcct ataattgcct gtatagaaaa tgaaactccc tcaatatttg
    50581 ccatccataa tgagccatgc agtacttcgc caacaccttt atcatcagcc ttttccttat
    50641 tataggtaag cccaattgat ataaaagaaa tattaaaaag atgaaatgca agcgtttcta
    50701 aaatatcggc tgataaaggt ataatttcgt aactccgaag aattaaacct attgcgcccc
    50761 cgattaaaca gcttggaatt aaaaatctct ggaatgcctt aacctttgct ctgaggataa
    50821 tgccaagcaa aagcattact gacatccagc caaatgcaat cattgcctca aacgtaaaag
    50881 gggtattcat atagcgccct ccatttttta tatatatttg taaaagttta caaatgaaaa
    50941 aagtatacga taatgcacct ttcagatgtt cagaacaacc tattcaaata atcttactgt
    51001 gtaataatac tgctgtcaat gtatttattg ctataaatag catacgtttg atacatcaaa
    51061 tttattataa ttttttattg attattttaa attaccaatt acaatcacaa caacatttgc
    51121 cttttagtaa ctatcagttt gatgtactat ttacaataaa aatcgcaata tctatattct
    51181 attggaggtt agtcatgaaa atcattgatc tttcatccat cattgaacca tcaccatcag
    51241 gaacacccga ttttttaaag attgatatca aatatcattc gcatggtgat ggagcaaaac
    51301 aggttgaggc acttttaaag gtaccagccc acctaatgcg taatggtgaa ggctgggcaa
    51361 ccgaaacaat tacccaatta ggcacccatg atagcaccca cgttgacgct ccatggcact
    51421 ataactcaac catccagggt aaacctgccc ctaccattga cgaacttcca cttgaatggt
    51481 tttatggcaa tggtgtaaag cttgatatga cccataaagc agaaggtgac cctgtaacac
    51541 ctcaagatat tgaaaaagaa ttgaaacgta ttaactatac tattcaaccc aatgacattg
    51601 ttttaatgta tacagggatg gacaaatttt atggacagcc tgattatatt ttcagggggt
    51661 gtggtgttac ctatgaagct actgtcatgc tgtataatgc tggtgttcgt gttatgggca
    51721 ttgatgcatg gggttgggat atgccactaa atcttcaggc acaaaaggca ttacaggaaa
    51781 ataaaccggg cattttctgg tcagctcatc aagctgattt aatatactcg catatggaaa
    51841 ggcttgttaa tttaggttta ttgccagcaa aaggatttaa ggttgcatgc tttccattaa
    51901 acatcaaacg tgcgagtgct gctcctgcac gggttgttgc tattgttgaa taatataatt
    51961 tccatataaa tttaatattg acaaactatc gaacaaaatc tggtatatat aaagaactta
    52021 taattattaa taattaaata agttcatgca taactgcaat ccaaaacgat tttaaatttc
    52081 atgttaaata attaaaatca ctatttatgg atgcagtcat aaaaacaatt aatataaaag
    52141 aggtaagcta tgggagatac tacacaaaac aaatgtcatt atatcattga aaatggaatc
    52201 gcaattatta caattgataa tccaccaatg aatgcattaa accatatcgt tttaaaggat
    52261 ttacgtaatt cagttaatgc acttattgat gatgccgccg tccgggtaat aatcataact
    52321 ggtgctggta aagcatttgt tgcaggtgct gacataaatg aaatcaaagt gcaaaacaca
    52381 aaagaagatg gtgcaaactt ccttaaaaac ggtcaggaaa tctacaacat aattgaaaat
    52441 gccgataagc catttattgc tgccattaac ggatattgtc tgggtggtgg tttagaatta
    52501 gcgttagcct gccatattcg tcttgctgat gaatctgcac aagtaggtct tcctgaaatt
    52561 aaattaggta ttattcctgg atacggtggt actcaacgaa gtcctcgttt atttggtaaa
    52621 gcagcagcat atgaattaat cctttcaggt aattttattt caggaaaaca ggccgaaaca
    52681 tatcgtattg tgaacagggc tacccaaaaa ggaacagttc ttaaagaagc tcaagaatta
    52741 gcaaaagcaa ttgctgctcg cagcaggctt gccgttaaag cagccatgca ggcaatagcg
    52801 cagggatata ccatggaatt ttatacagca ctaacctttg aacgtgagct ttttggtaaa
    52861 ctttgtgaga ccgaagataa aaaagaaggc attgccgcat tccttgaaaa acgcgagcct
    52921 caatttaaag acaaatagag ggatggacaa ccttatcatt cgtcatgctc attatgatga
    52981 gttagaagca attgctcgtt tagatgcact tgtttttggt caaaatgcgt ggccatttaa
    53041 tagttttgtc aatgaattgc agcttcattt ttcaaaatta ttaatagctg aattagatac
    53101 tacatttgca ggatttgcgt tagcgtggta tattgccgat gaaattcaac tgatgcgtat
    53161 tgctgtaaat cctgtcttcc agcgtaacaa aattggttca acacttattc aacatataac
    53221 tcattatgct caaaaccaga gcaaaattgt tttagaggtt gcagacaata atacacaagc
    53281 catagcattt tatacatcat taggttttta cagagtaggc gttagaaaaa attattatca
    53341 tttaaatagc gctattttaa tggaaaagat tattgatcat gaagattaat acagtacaat
    53401 ttataacaag tgctacaact ccgaatcact atccaaaata ttcgtttccc gaatttgcat
    53461 ttataggacg ttccaatgtg ggtaaatcat ctttattaaa tactatttta ggcaggaaaa
    53521 acatagcaaa aacgggtagt aaaccaggtg taacacaaac aataaatttt tattgcatca
    53581 atgatgccat aagcctggca gactgcccgg gatttggcta tgctaaaatt tcacaatcaa
    53641 agcgccgtga atttataaca atgatagata actatattaa tatacgatcg tcgttgaaat
    53701 tagtttttct tttaatagat atacggcgtg aacctgacga acatgagatt aatattataa
    53761 acaaatcgtt acataaaaat atcaatgtta tgattgtagc aactaaatgc gataaagttt
    53821 caaaaaacga aattaatgcg ggtatcaaaa ggatctgtac cagcttaaat ttgcatccgg
    53881 aatcggttat accttcttca aaaacaggca tgggtaaaaa tattatttta tcagttatta
    53941 gcaagcattg tcaataatgt gatgctcata gctgtgtcat caagtacatg accctgcttg
    54001 ctttttcttt tggcattaac gaaagcaggt acgttgaaat actcatcctt ccttcttttg
    54061 cagcattctg gtcaatctgg cgcaatacat ctattatgag cgtttcttcc cattgaacca
    54121 taatatcaac agcatcctgc gggggaatgc tttcaatctt ttttgctaaa tccataacat
    54181 tctttttata cccggaatac ttcttttttt ctaattccag agctttcttt tcctgatcaa
    54241 gccctttctg tagttgttct aatttttcct tttcttttga tattactttt tctgcctctg
    54301 ttatctttgc ttcgcgctta tccaattcct ctattcgttg caataattta tccttttctt
    54361 tttcaaactc ttcacgttct atcagcgatg gttcatcatc tgcagcatca acaacagatg
    54421 gagcctctgt aacaatatag tggtgatata ttttcccaag atttatcaag ccaatataat
    54481 ccatccagaa taatccaaca ataacaatga aaactatcat caatattaaa taaacaactt
    54541 ttgtggaatc agatactaat ttcatgttct actcctcgtg aattgctatc aacactgtta
    54601 ctcctcaata ttaaataaat cccccttgct ggtatattgt gatatagctt ttttcagcaa
    54661 attctgaatt tgttgcatat cagaggatgc cttactatcc gctttattat acaaaacact
    54721 tttcagtatt tcttctgtaa ttttattatc aaacgtatta tttttttgtt cgatagttcc
    54781 ttttatatca ggttgttggt tagtattttg ctgcattgca ttgggagtat tctgcactgt
    54841 tgttttttca gatggtgata ttctattata tgtcttttgt atcgcagcaa acctgctttt
    54901 tatttcagct attcttttct gcatctgttg tatactgttt atgtccatat atgcctcaca
    54961 ttgcattgta tattcgttga tttagatcat caagttcttt tcccatttct ctattaaact
    55021 gatacatata ttcttcatat tttttttctt ttaaacgttc aaccaccttt ctttcctttg
    55081 aagcttgcat taatttttga cgtaccctgt caatttcagg ctgcatctgt tccactcttt
    55141 tttttgataa gtctatagct ttttgagcgt tataagcaaa tcgttcgcac attaatgctt
    55201 cattatatga aaaggtaccg tccctcattt tttttgaaag tttattataa tattctttta
    55261 ttttttttct atactcttcc tgcattacta tctcttcgtt ttgttttgct ataagcatgg
    55321 ctagctcagc tttaattgca tgttcctttg aaatacgaat gtccaataat gtttgcaatc
    55381 tgaactgaaa ttttttcata acgtataatc tattttatta atttaacgat tcctttgtac
    55441 aaaatacggc tctctcgata caaaatctct tttttgctcc tgtttaactt ttgaaccaaa
    55501 cattaattct attaatttcg ttttaatatt ttcgtatata tccttttcat aaattccctg
    55561 tttaagaaat tcatccattg acggcttcat atctatagct gcatcaacat ctggatctga
    55621 acctcttgca taggcaccca gcattattaa atcttcagct tcatgataag cagctaaaag
    55681 tgtcctgaat ttgtttgccg cctttttatg ctcttcatct ataacatcat tcatacatcg
    55741 ggaaattgag cccaatacat caattgctgg ataatgattc ttatgagcta attttctatc
    55801 aagcacaata tgcccatcaa gaataccacg aacggcatct gcaatgggtt cattcatgtc
    55861 atcagcctca actaatatag aataaaaacc agttatactt cctgcacact ttttattacc
    55921 tgctcgttca agtaagcgcg gaagtaatga aaacactgat ggtggatatc ctcttgttgc
    55981 tgcaggttgc ccggcagcta aaccaacctc acgctgtgcc atagcaaatc gagtaatgga
    56041 gtccatcaat aaattaacat gcaacccctt atccctgaaa tattccgcaa ctgaatgagc
    56101 aagaaacccg gctcgtaaac gcaacattgg aggttgatca gatgttgaaa taatgacaac
    56161 tgatcgtttt aatccttcat tctgcaattc tttttcaaca aaatcttttg cttctcttcc
    56221 acgttcccct atcaacgcaa taacacttat atctgccgct gtatttcgtg caatcattga
    56281 aattaatgtt gacttcccta cccctgaacc agaaaaaatg ccaattcgct gacctttccc
    56341 aacagttata agtccatcga tagcacgtat cccggtagac agcggagaat caataatttc
    56401 cctttctaac ggtcttaacg gtttattgtc tacaggataa ccttctttta aataaatatt
    56461 ccctttaccg tctataggat tccccatccc atcaataacc cggccaacaa gttcatcacc
    56521 aacaggaaca ataagttgtg atcccactgc atacacatct gcaccaggaa cgacaccttt
    56581 catatcacca atcggcatta gtattatttt atttttatta aaaccaacta cctcagcatt
    56641 taaatatccg ccgtctttta atcttatgcg acataattct ccatattgca catccggtcc
    56701 atgtgattca atcgttaaac cggtaatttt ttcaactcgc cctgttgccc tgattgtttc
    56761 tacttcttca ataacttctt tgtactttgt aaatatgtcc accttttcat gtggtagtat
    56821 ctgaatcatt attgcctcgt taatctataa ttaaacatac cattataatg gtcgtgcatt
    56881 tctaattgct tcttcaatag cttcaagctg ggttgatatc cgtgcatcta tcgcacctac
    56941 atcagtttca atgatacacc caccccgttc aacacgcgag tcctcatata tatttacctt
    57001 tttaagcgat tccatcatct tgattaattc atctttatga gcggttgtca tatcaaggtc
    57061 agcaaagtta acacgtatat ctattctatc cctatcttta actcgttgca atgcttcttt
    57121 aatattatta ataacaacct ctcttctttc cactatctca tctttgatga ctttgcgtgc
    57181 tatcatcaag atcatctctg tcattaattt ttcagatgat cgtataatat catccctaat
    57241 atcaaccgca gtagatacaa tagttcccag cctgtcaata aggcgcatta cttcagcctg
    57301 cccttctttg tatccttctt ctcttcctgc gtcataacca cgtttatatg cttcatgctc
    57361 tatctcagat acctttaatt ctgcctcttt tatcattttt tcagcttcaa atttgccacg
    57421 ctcaacttct cgctcagatt caatctttaa gcgctccatt tcaattttta tctgttcacg
    57481 tgcctgctgt aactctaaga aggccgcttt tttaccatcc tcttcaattt gggcagctct
    57541 ggctttagct tcatcaagca ttcgacgcac ttcttcttct gtttcacgtc tataacgctc
    57601 taattctgct tccatctctt caatagatgg accctgataa acttccacgg gatttccttg
    57661 ctcatctact tcaaattcag catattcttc atcagttagt atatttttct gatatttatc
    57721 gggcaattca atctgtttgg gggttgcagt aatttgaatt tcagcaggtt taaatacaag
    57781 cttagacaac gagttcctcc tcgccagctc gtgctacaac aatatcgcca gcttcttcta
    57841 attttcttat tatattgact attttttgct gagcctcttc aacatcacgt aatctaatag
    57901 gacccataaa gtccatatct tctcttagaa gagcagaagc acgctttgac atatttctga
    57961 atatcttttc ctgtacttca gcatccacac cttttaatgc ttttgccaga tcctgagtat
    58021 caacttcacg caatactttc tgaattgctc tatcatcaag aagaacaata tcttcaaata
    58081 caaacatcct ctttttaatt tcttcagcta attctggatc ctcttcttcc agtgcctcaa
    58141 taataatctt ttcagtacca cgatcaacat gagtcaacac ctcaactatt gaatcaatcc
    58201 cacctgcaga ggtataatcc tcgctggcta atgtagacag ttttcgctcc agcactcttt
    58261 caacttcacg gagaacatca ggtgaagttc tatccatagt tgctatccgc cgcgctacat
    58321 ctgcttgaat ttgatgattt aatccagata aaatagttgc agctttttgc ggatccaaat
    58381 atgccagtat caaagcaata gtttgtggat gttcaccctg aataaagttt aagagatgac
    58441 ttgggtcggt cctgcgtata aaatcaaaag gcctaacctg caatgaggaa gtcaaacgat
    58501 taacaatatc aatagctttc tgagttccta acgcacgctc aagaacctcg cgagcataat
    58561 caattccacc tgtagcaatg aattcctgag ccatcataag ctcctggaat tccattaaaa
    58621 ctttatctct atcttcaggc tctactttat ctaaacgtgc tatctcaaag gttaactgct
    58681 ctatttcatc ttctctgagg tgcttaaata tttcagatga tacatctgat cctaacgaca
    58741 ccagaaatat cgcagccttt tgtctgcctg ttagctgtga cttcttttgc tgtaacatat
    58801 tctatctccg aattttgccg atatactgtt cccaaatcat tacctatctg tctgattata
    58861 gtatataccc cattctttca aaagtcaata cacgcttttt agtatatcgg cctatggcta
    58921 ttaaccaata acaacattat tcttctgcaa gccatgtcct caacagctgc gccacatcat
    58981 ccggtcgttc acgggctaaa ttaacggcat tctgctgcaa ctctaacctt gccttttctt
    59041 ctaatgacaa ttcaacatca attccttctt cttcagcagc acgtagggca gcttcccgca
    59101 tacgctgctg ctctaatgct aattgttcct cgcgtatacg gcgtctgcgt gcaagttcac
    59161 gggatatagc cctgaaaagt atcacgccta aaaacaacgc cattatacca accagcgctg
    59221 ccaaaagcat gcgtttaaac tgctcttttt tcctaaattc ctcacgagca atatcccatt
    59281 gttttgttct atcaaacata atattttcaa cagccacctg atcaccacgt gcagcattaa
    59341 aattaatagc tttctttaca atattttcag cctgccgtag ctcatcctcg ctcaacggta
    59401 gctgaacagg ttttttagca ggatcaagat catagctgcc atcagggaga cgtggtaaat
    59461 cctgataacc atcaatagct attgcaactg atactgctga aatgtcaaaa ggatctctct
    59521 ctattttacg aatagtttta ttgatcgcat tattttcaac attttcttct ttatcgtatt
    59581 gcgaaaattg gtcatcactt gctttatatc ctggtggttt attaccttcc gttccagcag
    59641 gaccttccgg attccaacca tgtccttgaa aatgttcttt agtactcttt tttgaaatag
    59701 tcaaagcatc tttaaccttt cgttcagaat acggcgttag tggattatcc ttttccatct
    59761 caataggagt atattcttcc tgttcttcct gaattttatc ccagttaaaa tccatgttaa
    59821 gccttactat ctgtatacga tcagaagaaa aaatcttttc cagtcccaga cgtatatcct
    59881 ttaacatctg gacacgtgct ttttcttcta ttttttttct atattcaaga agggtaaatt
    59941 cctcttttgc cttatcaaat tggtcatcaa aatccgagat tatttttcca tattcatctg
    60001 ttatagatac attttccggc tttaatttag taccaactgc tcttgatact aaatactgta
    60061 tacctttgat ttctttttta gacagagtat catacccggg tgcaaggtaa actgtaacag
    60121 cagccgtata ggcattttct ttttctgtaa aaagctcatc ttccgtaata gctatttcaa
    60181 cgtcagcctt ttcaatattt tttaatgatt caatgtgacg tttaatttca tcccgcaatg
    60241 ccctcatata tttaacattc agctctttat cagtctcggt ccattttgat atatcaaaaa
    60301 gtttccagcc aggtatccct cttggtatca tattctcctg tgcaagacgg gttaaaataa
    60361 cttcacgctg attgggatct accagaatag tggttgttcc tgatgttgta tagtggaaac
    60421 ccatttcttc caattttttt gttacctgcc caaattcatc agaaggaaga tcagagaata
    60481 aaactatatt tgctttctgt gacgacaccg agaataaaac aataaatgca ataacaagaa
    60541 cccctgttac cacaccaata ataatttttt ttgttgtatc taatttagca taaatctctt
    60601 tcaattgttg aaatatcttg gtaaataaat ccatatcttc ttccctccca caactaatta
    60661 aatatcatca ccttgctcca caataaccat gcttcacatt acaaaatgat tattactgca
    60721 ataacatcat caacatatca attgactgca aagtgacata atacatatag cattcacaca
    60781 taataaaaca tcagtattaa aaagtcaatt aatttactaa aatttgctac tttttttatt
    60841 atattcacag caatcacata ttatctgaga ttaatgagtt ccctgtatgc cctgatcgct
    60901 tcatcccgga cagcttttgt taagcttaat gcaatctcag ctttctgttg agcaatcatc
    60961 actgtatgta tgtctactga ctcaggctca taaatcatct gttgtgtcaa atcttctgca
    61021 tcaacctgta aatcatttac cttccccaat gttgatttca gcgcatccat aaaagaacct
    61081 gcaacatcat cgtcctgaat tttttctggt ttattgtcat aatggagagg atgcgttgtc
    61141 ttcattgtaa aaatatttga tttacttata aaattacttt cctgtaccat aattaacgct
    61201 ccctgaaatt attcttgatt caacaatttg tcgctcacca ataccaaact gcagccattc
    61261 cttttttcgt ctgcttaacg taatgggagt cgatagttca tgtgaacaac aaatttcatt
    61321 tatatgccct ttttcaaaat acatatctta aactccttcc gtaaaaatta tacaccaccc
    61381 ttgccaattt ccagcgcctt tgaaaacatg gattttgcat tattaatcat catgacattt
    61441 gcttcatatg aacgcgatgc agagattaaa tcagtcatct ccatgaccac attaacattt
    61501 ggcatttcaa catatccctg ttttggaccg gtcttaattg catcgggatg tgctggatca
    61561 tatatcagtc tgaattcagc attatcttct tcaattttca caactcgcac acctttaggt
    61621 tctccatgat caattctttt aggaacaagt ggcgatttgt aaaatggcct tatattgaca
    61681 ggagcaaata tcacccgttt acgtttaaat ggaccaccct ccggggtacg agttgttgtt
    61741 gcatttgcaa tattattgct tataacatcc atgcgcaggc gctgtgcagt caagcctgtt
    61801 gatgctatgt taaaactatc aaacataccc atatcaaacc ctcactaaac agttctcatt
    61861 accatcttta aaattttata attttcattt aagcttgaaa tatatgcatt gtatctcatc
    61921 tgattttttg cagcatcaac catctctttt tctacatcca cattgttccc atcatttcta
    61981 tacgtagtgg tataatctac attaattctt ggttttacgc tttgaatgtt ttttggttca
    62041 aaaaaatcaa aatgacgagg atccgttttc aatgctttca ccttttgcat ggaatcctta
    62101 ttatgttcta ttgccctttt caattcactt tcaaaattaa cttcacttcg cttaaaatga
    62161 ggaacatcaa cattggcaat attatttgca ataatcttgc ggcgtattga ttcaacatcc
    62221 attgcctgtt ctaaaagata gttggtttgc atcattttac tatattcaaa catact
//
LOCUS       DAOOJH010000005        57754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_635_length_57754_cov_10.132394, whole genome shotgun sequence.
ACCESSION   DAOOJH010000005 DAOOJH010000000
VERSION     DAOOJH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 57754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57754
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_635_length_57754_cov_10.132394"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PLJ75_01320"
     CDS             complement(<1..1257)
                     /locus_tag="PLJ75_01320"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPD03892.1"
                     /translation="MQNNRVTITILPENRSVTVPAGTTIADALLACNIILHTPCGGKG
                     ICKKCTVHASGLLSQPDDYEKSLGDSSLRLACRAVIIGDAYIWIDSITQRPRYPELNP
                     THTYAVAIDIGTTTVQIAFVDMTTKQAMPEITLLNPQRRFGHDVMTRISNANDPAKAS
                     QLTQILLQTIITTIQDVCNISGIDIHSIVTIAISGNTVMSYSFLGIDITPLGTHPYTT
                     LITSFDSYNHYPPLKKTFPNAQITVLPAVSAFLGGDFSGGVGTLPKTKGNSFFFDIGT
                     NGEMAIIKEDGTIVATSCAMGPALEGMNISSGMTATEGAITHFTLYNNTLTYTSLGDG
                     KPSGISGTALIDLIAILLRNGVIEKSGAIKTAIPQPLSEHIKLEHIETMKALNILNII
                     TINQQDIRNVQLAKGASLAAAKILLRE"
     gene            1416..2549
                     /locus_tag="PLJ75_01325"
     CDS             1416..2549
                     /locus_tag="PLJ75_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03893.1"
                     /translation="MDATIISYPDTSVVQLLSLTENRSKYMLPFAGKFRVVDFVIGNA
                     KEVDAHTIIYSNVNDDLANYVQLHGIDESRLKVVVDNFNSVDFFYSIVRDSDSAYFII
                     YNGDNPGIINFSHLIERFITIKKPSVLYKMHFEEKARMAHRILVTTKKALLDTIRKAL
                     KHNHSAPNIFEMIINMMVNIGLAKGEFSCMYWPFSTIVDYYNHNFDVINNADYSKHVF
                     DGHINAKLELYRYSMLGPDARISHSFLSDSTYINGEVVNSIIFPGVEIGKDAVVRDSI
                     ILPYNKIGSKARIMRTIIDERTVEPFSADIINIGNNCKIGTTEIGMKNSDYPRVLYNS
                     ITLIGKNCMIPDGITVGGACYIASGRTQQDFAKRKYIYNGTSV"
     gene            2533..3192
                     /locus_tag="PLJ75_01330"
     CDS             2533..3192
                     /locus_tag="PLJ75_01330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPD03894.1"
                     /translation="MAPRCDMFFPYSDENPTRSFSLFTVILILANCVIYYMEVYGQPA
                     FAQLINSYALVPCNVNKLHLDDYRYYVPFISHMFLHVNIIHVAGNMLFLWIFGNNVED
                     VLGGIRFYAFYVLCGLAAALTFYLFHHTECTPIIGASGAVSGVLGGYVVLFPSARVKV
                     WFIFFVLRLRAWFFILLWFLLQLSNLYGEQSGVSNVAWSAHVGGFIAGFVLIMFFKKR
                     Y"
     gene            3233..4843
                     /locus_tag="PLJ75_01335"
     CDS             3233..4843
                     /locus_tag="PLJ75_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD03895.1"
                     /translation="MKMYNKIEKYLCVAVFLLFSIRWLPVYADVAVIPYKVESADTSF
                     TEAMGSEYAKLVALAMYIQKGISIYSHDLLEKDMHEFSIDPQGVVAGYKLDTLGKARS
                     IDRIQIGTLTKTSKGFTVKSLLYDVATGKIVSRCNEYADTLYDVAIAEMRSLYLSYPD
                     NNRAMQQSIYDIAFVVDNSYSASREWKSIKQGIVALCDTISDNWNDMRVYVVPFLTQS
                     KKIGTYAVTGATTLDDVLQELSLAKGIVTGINPCLQYIAKGLPWRNDAKKLCIVVAAS
                     RCDYSNARSLRYLIKKKGITLYVIGTGGLTHTERTALSFIGDSYYDVTYRQRMYDVYG
                     NPVDVFCEAGRIFHGDAGDRWKDGVTATKTKAAKPFVAEVFGVQNALPYELPALYPKQ
                     TAIKILNAEELENNIVDICQTIAHNNKLTGQVIARVLIADGGYSLWLPVADKRILNYL
                     AENQHMRMYVGISPKQDLGAPYGVGLLPVAVVGLPGSHIPSMLKLTVKDIIQRPQWKG
                     FFNPPLWFIPVTVKQIIPLSGEEDIRNK"
     gene            5012..7621
                     /gene="clpB"
                     /locus_tag="PLJ75_01340"
     CDS             5012..7621
                     /gene="clpB"
                     /locus_tag="PLJ75_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPD03896.1"
                     /translation="MRMDKLTLKTQNALYEAQNVATQYNHPEITPEHVLYALVNQDDG
                     IFTPIAQRLGASVDEIKRETEMALSAMPKQYGGPGGGVMSSVLRDVLNTAWNEAVTLK
                     DEYLSTEHLILGIATTGKTKARDILNAHGFTKENILRVLADIRGNKRVTDESAEDKYN
                     ALERFGRDLTKLARQNKLDPVVGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIVE
                     GLAQRIVAGDVPESLKNKRIVALDMGALIAGAKYRGEFEERLKAVIEEVEQSEGDVIL
                     FIDELHVLVGAGAAEGSVDASNMLKPALARGELRCIGATTLNEYQKYIEKDKALERRF
                     QPVFTSEPSVEDTIAILRGLKERYEVHHGVRISDSAVVAAAVLSNRYITDRFLPDKAV
                     DLIDEAASLIKMEIDSMPIELDEIERKIRQLTIEREAVKKEKDKASLERLAKIEEDLA
                     NLNEKKKELSVQWQNEKKIIVEIRKIKEEIENLRIQEQKAEREGNLNLVAEIRYGKII
                     ELEKKLKALNDKLNDIQKDRKLLKEEVTEQEIAAVVSRWTGIPVSKMLEGEKEKLLKM
                     EEIIEKRVVGQEQAIQTIADAVRRSRSGLQDPNRPIGSFIFLGPTGVGKTETAKAVAE
                     FLFNDEKAIVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGQLTEAIRRRPYSVVLFDE
                     IEKAHPDVFNVFLQILDEGRLTDAKGRTVDFKNTLIIMTSNIGTAYISDPAIPYEERV
                     KGVQQELKMHFKPEFLNRIDDVIVFNPLTREHINKIIMIQLNEVGKRLKERGIKLELT
                     QKALDYLVNVGFDPQFGARPLKRALQRYLLNPLSVKILGGEIMDDDTVIVDYKNNEIQ
                     FNVKRSKAEKQAV"
     gene            7906..12303
                     /locus_tag="PLJ75_01345"
     CDS             7906..12303
                     /locus_tag="PLJ75_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD03897.1"
                     /translation="MEYEIYDVNNILLPVEHKIGALSRAKNLVAEMTHPNIDWKYMVT
                     EIRAYLYDYMYDIVPHSDRVLPVIFHYLKEATVRKRGSTIRAADTFLDRYLFLLKKEI
                     AGDSSLDNVTAIFDKEAIDFCRILLSDTQEGFYLEDVNQRIIQLMELSLQRKKLDAQL
                     FELCTDIIINQFKLYVERSIVVEEEYMDSLRNLWNSDGESIATLRQYVQTVTKEAYEK
                     KLQIALDSRTTITDYEKLLQQLINVIDFHHNETAWEKICIAARECIVRNIIQDDSVLL
                     SLLSFMVKKSQQGNDTNLQLYISRSVASLCGVLAQQQRFDLLKQVVQMVVPVLISEIE
                     RGGNYNAAFATIYTIGKTVVEINNRPIIDLFVDILVHARFCFPQFTGIASDWSVMVNA
                     SHLANIRTWLELIELNPVYMKRLAASLIANLTLGGVFLKDTDVFQRDISRLLNSNYKD
                     VFYLIISLAAVFPAFYHDIGATGNIRAFTERVDTNHQMNDLIHFVRKQVHVESSSRTV
                     ALLQRVMDFWLTGDKELLKGMVPLEVYNNLDRTFRLINLDNESIARRIYMETRHYFPE
                     YVNEKFWDFFYKVGKKRFMDAIAHHTFDGMDENEKKDALDCIVEYFDKQFPAEMTKML
                     HHIAGMFDIDTSRKQIWRFLYEIPDDDFRKLFEDVQRLDVSTVNIEKFITFLHVYRMI
                     FDKYNFSDIRAIEKLHQYASENLFNPPDDFFQKLEGNDLFQSLQAILDLQNRLKWDIL
                     LSQDVFEPVDTIEFKRHIAFGIPSMYGSYKEKKFDTLKVFFHCNIVRVLLFEKIIENI
                     SIYPHQKIDYDAIKRVVELFIQSFEIDGLANHEMRSVTSLLDAPNITVTQFRDIVNSL
                     LVIHGEISDRFNDTFKSVSRIAIKNIGMDNIIPDFIPPDQPANVEVIVDRFLRHRVMQ
                     SPLLQLMDNLLLKLRDHLNSVYSYMGNIVVLGAIDARMKKGKLIHVIGKYEGTYDETE
                     LYVPLWEVGAKAQGLIIAANMDGINVPEGIVISSELYKRIKDGNINNPRFKRKLIYML
                     KKYIDAFTGFRFANPQNPILLSVRSGAVFSMPGVMDTITNVGMTEEIVQYFTQFDEWF
                     AWDCYRRLIHDFAISAYGMDRHIFENLMTQAKDEAGVDLKEKLNGKQMSMLTLKYRYA
                     INKAGHSAPKDPYEQLFYAIIAVFKSWDSPIARNYRQFINISDDWGTAVIAQRMVFGN
                     LSPTSITGVVHSQYIEYEELQIVGEYKTRAQGHDIVSGVAKVFPINEQQKLKFARFAM
                     YPSLEKAYPNHYATLRDAVSRLRKRWGNDIEVEFTFENDVLYILQIRGMAKHMFDIEE
                     LVETPQQLSQYLLGQGLAASGGAVSGRAVFDINRIEAIRMQYPKDKIILIRPETNPED
                     VIGLQKSDGILTSVGGMTSHAVLQMRRLEKSGVSDFSIMKIAESENIAVINREQGGKI
                     VIREGDVITIDGNTGHVYLGAHPTRKVHR"
     gene            12482..13630
                     /locus_tag="PLJ75_01350"
     CDS             12482..13630
                     /locus_tag="PLJ75_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HPD03898.1"
                     /translation="MAVLPDAPIGVMGIGRIGKLLVWLYAAKKQQKEIVIATGRKTGT
                     SLEDLATYFEYDSTYGSFNRFIGGFSGKSAVEIKDGHVYLNGVKLIWLNEPDYRVPGK
                     IPWDTFGVNVVFDTTGKMLDPTRNDENSLRGHLNHAKKVIVTAPFKVKDKGKQIPDDA
                     VTVVGGVNFDKYDAGKHVIISNASCTTNCCAPPIKALVDHFGDKFISYALTTVHAVTN
                     SQSTLDVLPKDGDKDTRKRRSILNNMIPTSTGAAKAVLEVIPEVQKLGIGSQASSIRV
                     PVNTGSIVILDVSLLGDYDNQYIHKIFKQYSEKNPDIMCYSEKQLVSSDIVGSTYSTI
                     YDAEFTHHRTTKRGDNYYTMVDLNFWYDNEFGYVSSLSRLYDLVTGRR"
     gene            13785..14198
                     /locus_tag="PLJ75_01355"
     CDS             13785..14198
                     /locus_tag="PLJ75_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03899.1"
                     /translation="MKRVGFGFLIVLIFYNATTAFSKEVPFTLEDRDRIIRLEAKLDE
                     IDKRFEQIDKRFEQIDKRFEQVISFLWILTTIFIAITSATIGFAFWDRKTSLKPIEEK
                     VTINEKKLEHALAALRKLASVDKKVHAILKEFNLL"
     gene            14577..14801
                     /locus_tag="PLJ75_01360"
     CDS             14577..14801
                     /locus_tag="PLJ75_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03900.1"
                     /translation="MKKFVIVFALVFFVCAIAQHSISISLNPKRVACEQSCTEGKKKC
                     FADAKDSEVKKTACKVAFDECMQKCAKDFQ"
     gene            15251..16462
                     /locus_tag="PLJ75_01365"
     CDS             15251..16462
                     /locus_tag="PLJ75_01365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD03901.1"
                     /translation="MKRKIVITVSLVISLLVIVYAQNTNRLKESGFNNAFTLPPETPY
                     EQYVELMTKVIAAARLETDNKEAIVAMNSPFVYKPAEKPVNNIYSKGILLIHGLSDSP
                     YFLQAIGKYFASKGFLVYGLLLPGHGTVPGDLLKVTYDEWIKATHYGMLQLKKNAQEV
                     YMGGFSTGATLSVNYVLSNTNHDVKGLFLFAPAFAINSSVAWLAPIVSTFKDWTDIDD
                     DVDYTKYQSLTLNGAAQTYKLIKKVDSYFEEGKTISVPVFVVQSIDDETVKAERTLYV
                     MKNYVTGKKMFVLYTTQPEKDYDGEGNFVVTVNSFLPKEKILNFPHICVTIPPDDPHY
                     GRNGDYRNCLYYKQGSPERNKCLTDPNIWKGETTAENIEKYKVIARLTYNPYFDEMCQ
                     KIVEFLNIIKK"
     assembly_gap    16914..17013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17167..19632
                     /locus_tag="PLJ75_01370"
     CDS             17167..19632
                     /locus_tag="PLJ75_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD03902.1"
                     /translation="MDYLKLYRRFYLDMLIHGNALSAIIGAPLTVLFFILQTEITLSN
                     LHVVVVPGIIVFVVVTLMTYVWYQYLFAPFRWFFTAKAKGTQFSEKHALMLKERMSSV
                     AVLFSLGLIGQWTIGLAVFSITSALLYGLTFDQLLYLWFGGILSVGISIPFGSYFISK
                     TIKRLTDDGLFDEVKNVISVKPVTTFGSLIPILTGSTSMVNVLLVIVLVTFGIVLSHG
                     YMKTMFAQNINSISNQAAHKLDDMIDGVIIDCKLVSLLPDRNMINRIVSLKKYYADYA
                     IIENGNVTTSGTGRYDAIIEKGMLSRKSLGQDGEFITKPIVYEKDKKAYVVIGDTING
                     RIYAGLIAVDAIKEQIFKDVTIGRTGYIFVTYEDGSGLVHPQIDITSDALTHYEWSKK
                     LINNHNTLIAYKFNNDDNYGYCTKSGRGFLLIANIPKSELNEYSKLLALLSLGVGLFW
                     CVVATVILYRTNRGHLEPLKNAREVISKVAQGDLTQNLDIIKGDEVGILSSAVNILIK
                     ELREAIAHIIRISDELASSSTQMSQSITSFADNVQNEASTVEEISATVEEISAGMENV
                     ARRADGQNQSLSLLINLLNNLSTTIQTVADTMNASVKEVELIADRSREGSQSLNQMNS
                     TMQVLSKSSADMKNIVKIINDISEQINLLSLNAAIEAARAGDFGRGFAVVADEISKLA
                     EQTAQSIKEIDNLIKQNSTEMTKGTQNITQAIQNIGFIINGVKDISTHIEHVASEMKE
                     QLAINTNVQNMIADVQSGSEEIKNATEEQRVAILEISRSITNINDLSQSNAGAAEEMS
                     ANATNVESMAVQLKEVMAFFKIS"
     assembly_gap    19640..19739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19909..20958
                     /locus_tag="PLJ75_01375"
     CDS             19909..20958
                     /locus_tag="PLJ75_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPD03903.1"
                     /translation="MSLSLGIAGYQQSGKKTLLQLLTGNSFDMKKGLGVATIHDQRFR
                     TLVDIYKPRKVAPATINISLIGDIDDDIIKEGSIFNTIATMDALCVVIRAFTSDSVYH
                     IKGSVDPFRDYDKLISEFVLHDMMFAEMRINRLQQDKKKTAQQKEHEIALLNKFLQHL
                     ESGTLLNGLQLSNDEIAIIKSYPFITLKQLIIVFNTNDNDSGIINKFKAMFPEHAVYA
                     IAIPVKLENEINELASEKERMEFYASLNIDAPAINLLCNAMIHALGLQSFFTVGQDEV
                     KQWLIPEGITAPVAAGYIHSDIQRGFIRAELMHYDDFIVAGSEELLKKQGKFHLAGKD
                     YIMNDGDIVSFRFNV"
     gene            20973..21848
                     /locus_tag="PLJ75_01380"
     CDS             20973..21848
                     /locus_tag="PLJ75_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPD03904.1"
                     /translation="MNDILKELTHIRELYSETGAVDTIITNEFVTCIYNFFDSNYRNL
                     PWRNTSNAYYILVSEIMLQQTQVSRVIDKYKEFIKIFPTIHALADADLYDVLSAWQGL
                     GYNRRAKYLWECAKAIILQYNGLIPHDIMELQKLPGLGKATAASVAIFAYNIPAIYIE
                     TNVRTVYIYSFLYDTAKVSDAAIALLLQQTMDRLNPRKFYNALMDCGTFIKQHYNASR
                     HSEQYTIQSPFNSSLRKVRGQIIRYLVDKKKARKEELVMYLDCDRDKIEKALHSLCKE
                     KLVCEDNNIYTISRS"
     gene            21943..22746
                     /locus_tag="PLJ75_01385"
     CDS             21943..22746
                     /locus_tag="PLJ75_01385"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HPD03905.1"
                     /translation="MLKRSIFFVLFFFIICTSVNVFAVPVAYRSWTYLDLTALLSPEW
                     SWTLLSSHSYEFDRNNDPKGNVEKKTFFYEVFTGPTYTMKFGNLTVKLPLWYYYQGFP
                     IRSAVNPSDHDKYYYSHNIEFMPTLEYKIGNFKFWNRVIFHNKVYSSLYDSSSKNKGY
                     SLLIREYFRVEYNVTDQFAVMIGDEIFQGIIEDDDTKDITPSPLSPGFEYGGFTQNRL
                     YIGFSYKCTPTIIITPMYMYQTQYFDAGNELTEKDHYLFTTLTFIFKTY"
     gene            22897..23670
                     /locus_tag="PLJ75_01390"
     CDS             22897..23670
                     /locus_tag="PLJ75_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03906.1"
                     /translation="MSFDSLQKLFEFDALLHMVIEPASRFEPVNSRLVDCIEVYKPRV
                     IVKIGLGNPAYIESIMEHSKALVVVVEPSVTVLQSFYNAYGNKQWMNRLYTIAGNFSQ
                     FPVDYYKADMIICIDCLDLLDTGRAIDEFRRSLQFEGVFFYAGFVLPNEDAEGIFDEI
                     AHVINPLHTDYYLEDDLKTFMTLNEFGIVKLNTQKINMNILLLADYLQTRSSELEKLI
                     EEHKDTLTALYDLHSDGTVVLPYMIAAFIRQKPSQEEAL"
     gene            complement(23652..24950)
                     /locus_tag="PLJ75_01395"
     CDS             complement(23652..24950)
                     /locus_tag="PLJ75_01395"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD03907.1"
                     /translation="MQSVSLVKYSKSTDSLKQAIELCDGFSKLKPGDKVLIKPNLVAW
                     DELFPPAPYGVFTTTRMVEDLIIILKEYGCNDITIGEGSVEVKKGVGTMAAFAGLGYT
                     DLASHYGVKLVDFNESKSEKCSIDASTHLMIAKEALECDFFINFPVLKTHGQTKISLG
                     LKNLKGCLKASSKKLCHHPQLNLEYCFPFVADYVKPELTIIDGIYALEKGALHFGNAY
                     KKDIIIASTDILAADIIGAKVMGYEPSDIAHFVTYATRHNKSLVLSDYAVKGEKLEDH
                     IQPLKWDWSWTADNTGPGAFAKMGIQGVALPKYDDTLCSGCSPIANMCNILVMSAFKG
                     QPLPKVEILNGKKMQARPGYDKTVLLGNCIIKANKNNPTIKESVEVKGCPPNFDDVVK
                     ALKTCGLEVNEMAYLGYMKQQSEKYNGKEGYDPLYYRASS"
     gene            complement(24983..25150)
                     /locus_tag="PLJ75_01400"
     CDS             complement(24983..25150)
                     /locus_tag="PLJ75_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03908.1"
                     /translation="MVQVDIVWSYAFGASFAAVAAHQLKIVKHFYKLWNCIFTFAIFK
                     KVLHIYHDYFL"
     gene            complement(25164..>25842)
                     /locus_tag="PLJ75_01405"
     CDS             complement(25164..>25842)
                     /locus_tag="PLJ75_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03909.1"
                     /translation="KWIEYAPDWKLLKKKDDVLLYTRKLPSGIVQVKAEKIMYVKPEV
                     VEQLVRDVPAYKEFMYKCFIAETIKIFNDEDMIIHNATAMPWPLKPRDGVVRTQVTKD
                     WDYGRFEVLCYGLLPPESEQYVPQVEGFVRMYEVSAYMIAHILQREKVKCFYIIHADP
                     TGVPLFAVNHLIDSYPYYTIIKFEEMLKKPEYIDRGKNSKYLAQIEAFCKQKQSALPK
                     TNQHILP"
     assembly_gap    25843..25942
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25943..26140)
                     /locus_tag="PLJ75_01410"
     CDS             complement(<25943..26140)
                     /locus_tag="PLJ75_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03910.1"
                     /translation="MHTCLKWYIPLFVIVVCIPVYDSAVSSQNQITCLHEDWTECASN
                     DIKSKWIEYAPDWKLLKKKDDV"
     gene            complement(26306..26494)
                     /locus_tag="PLJ75_01415"
     CDS             complement(26306..26494)
                     /locus_tag="PLJ75_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03911.1"
                     /translation="MKMNGITASFKTLYVILVIVTLTAGSIALSGCDNYDIDESAVQK
                     IDCPDEYSVLPWQWDLKI"
     gene            26775..28016
                     /locus_tag="PLJ75_01420"
     CDS             26775..28016
                     /locus_tag="PLJ75_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD03912.1"
                     /translation="MKARISISLFLLGLGGQIAWAIENQFFNTFLYDRIIPDPKYVSI
                     MVALSAVTATVTAIIMGAFSDSIGKRKPFLLYGYLAWSVSIWVIPMAEWIKPVIVAAW
                     MVIILDSVMTFFGSTSYDAVYNAYLTDVTTDANRGKAQGLLSLATWIALLLVYGASGQ
                     IVESMGYFFFFGAAGALVLLSGVIGGIAAVEPQRAIVTDSKSIVKRIRGSLNMQALRH
                     NKNLLYVLLAIGFWGMAFNVFFPYLLIYLKHFLKIDIATSSVIIAVSIFIGGIAASIP
                     AGIIADKIGRKPVAIFAVILISFSLIAFSYARLPIAIGILATLWIIAQTIWTVATGAW
                     SKDLFPEERRGEFAGYFTLFYVAFTMIPGPLIGAIVADRWGIRATIDGKPGIIPTPEI
                     FVVSAVLILITIIPLLFAQEQ"
     gene            complement(28028..28651)
                     /locus_tag="PLJ75_01425"
     CDS             complement(28028..28651)
                     /locus_tag="PLJ75_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03913.1"
                     /translation="MKKFIFILSVVTLFTIALYFNVSLTHAQSNSIVFLSPEFTKFVC
                     DEWNRSELTQKLGTKAVGGNDWILTKNKYTGQVRNKQVFAMSRRDCDSMPKVQLTIEN
                     KDGRAICTYGGALVEEYKTVEWAFAPTTVQWYKFASGVWGYMQMPGIMKGFRGPMFVA
                     RANIDNFGIFWKVVGKAAKQINADYKAPCALSQDDIDAIEKYIKNTQ"
     gene            29131..30255
                     /locus_tag="PLJ75_01430"
     CDS             29131..30255
                     /locus_tag="PLJ75_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD03914.1"
                     /translation="MKRFIMYSIAGIILVIAVSISYLIITEYILIRFPVLVQTNPKIT
                     FIIGDSYFREDEKAQWKIAVVGQTLYQGYWVKTDPDSRMDIRFHDNTAIQLSESSEMA
                     IDSLTVKKLGLQIHTGSMYGKFDKLFKEHDLHVKTPTAFAAIRGTDLGFMVVPESYGN
                     DSLMGQQKKSAYNKGKNKGQTLKTKGATQNPDSGEADLQTLVYSLSGITEIYNPIFDD
                     QKVLLSYRNKVIIGEESTPTNPEELSEDEIKTLQEKLNSIHFDEVLLITDKITFNFGS
                     AEILPSSYPELDKIVEILKQKKVRVRIEGHTDDQGDAAFNQQLSEKRAEAIKNYLVTK
                     GLDPERFETVGFGESKPIADNKNEVGRSQNRRVEFLIIEK"
     gene            30313..30395
                     /locus_tag="PLJ75_01435"
     tRNA            30313..30395
                     /locus_tag="PLJ75_01435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30347..30349,aa:Ser,seq:tga)
     gene            30402..30488
                     /locus_tag="PLJ75_01440"
     tRNA            30402..30488
                     /locus_tag="PLJ75_01440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30436..30438,aa:Ser,seq:gct)
     gene            30556..30936
                     /locus_tag="PLJ75_01445"
     CDS             30556..30936
                     /locus_tag="PLJ75_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03915.1"
                     /translation="MHYYAVVKQFCFEHKQQIIASIAYIPYIGWLYPLYACEGDGVIQ
                     KHAKRGLLYAGVFVFAALVLFLLSFILPRSWRLLSFIVTIVLYINYFIYYAIVIIHIF
                     NIWKGKDFSIPKFEIYLEKIEQYI"
     gene            30962..31035
                     /locus_tag="PLJ75_01450"
     tRNA            30962..31035
                     /locus_tag="PLJ75_01450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30996..30998,aa:Arg,seq:acg)
     gene            complement(31135..31623)
                     /locus_tag="PLJ75_01455"
     CDS             complement(31135..31623)
                     /locus_tag="PLJ75_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03916.1"
                     /translation="MNFETTTCISSNHIIVLKHYAKILDVSVSKLISYLIQYAAKKEK
                     RKTQVFKNISYRDRNTDIKWIRFHLCISWSEYEFLLDIKKIWKVSIAKAIEFCVDTVL
                     DEFFNYVIHRRKESLTDNYLSYYENRSYTFDFFKVKGIHCCQFYWGPPPEILKINYGD
                     LH"
     gene            complement(31815..32882)
                     /locus_tag="PLJ75_01460"
     CDS             complement(31815..32882)
                     /locus_tag="PLJ75_01460"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPD03917.1"
                     /translation="MKKSYNIAVIPGDGTGPEVVAEGVKVLKAVAGVENISLHFTNYD
                     FGGERYLKTGETLPEGAIDELKKHDAIFLGAIGHPDVKPGILEKGILLALRFELDQYI
                     NLRPIKLYPNVETPLKDKGPEHIDYIVVRENSGDVYTGIGGVTMKGTPHEEAMQIMIY
                     NRFQVERCLRYAFEYARKRNKRKTLALVGKTNVLTYVYDLWERAFHEIGQKEYPDIKR
                     DYYHVDATCMWMVKNPEWFDVLVTGNMFGDIITDLGAMTQGGMGIAAGGNINPEGVSM
                     FEPIGGSAPKYTGKNVINPLAAICAAQMMMEHLGEEKTAARIEKGVIDVLQRDMKSLA
                     AGKMGLTTTQVGDKVAEYAVS"
     gene            complement(33341..34030)
                     /locus_tag="PLJ75_01465"
     CDS             complement(33341..34030)
                     /locus_tag="PLJ75_01465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPD03918.1"
                     /translation="MHNIITVTLRLLILATPLLLNDCYLPLIPKDATKLNLILDIIIY
                     IGWQTSLLYVAYDAGWFTWERLGINVKQPKQWLWGVVLFIAIFVAYAILTIALEYCKK
                     HYGFTLASFWYFPLPGWKPLYVFLYALYLSITAGIYEEIIYRGIAIHQLRLITSNKWI
                     LIIASTLLFVAIHWSMGPGTWLEAALWGALWAYLYIQTNSLLPIMVAHFLYDLTTLYG
                     LHLAIAHCIGL"
     gene            complement(34040..35377)
                     /locus_tag="PLJ75_01470"
     CDS             complement(34040..35377)
                     /locus_tag="PLJ75_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD03919.1"
                     /translation="MNLLSLFAPPPAKPQLPEEKVKKLYPRLRWHILQATFIGYATFY
                     IVRNNLSTVARDMQTAFGYDHSQIGSILAISALLYGIGKFLLGSVSDRSNVRVFMPVG
                     LILTALCNFAFGATQSYAIHFWLWALNGFIQGAGWPPCGRSIGHWFSVSERGTIFSVW
                     NIAHNVGGGVAGMLAAYCAKHFGLHNAFFIPGAIAILCALYLFWQLRDTPQSVGLPPV
                     EVYKNDYPPQTHEHIEKELSTKTLFVDYILKNKLLWVVAIANFFVYIARYSMLDWGPT
                     FLREIKGANITQGGFSIMVLEFAGIPSTILFGWISDKLQGRRGMVSMLCMIPVVLAFF
                     AIVVYPQPSLWFYYAMLSVIGFFVYPPVMMLGVMGLDLTNKKAVGTAAGFIGMFGYLG
                     RTVQAKGFGFILDYFATTYDIVTRWNIVLYSIVACTVISIVLLAFTWKVKPRG"
     gene            complement(35629..36963)
                     /locus_tag="PLJ75_01475"
     CDS             complement(35629..36963)
                     /locus_tag="PLJ75_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD03920.1"
                     /translation="MNLLSLFAPPPAKPQLPEEKVKKLYPRLRWHILQATFIGYATFY
                     IVRNNLSTVARDMQTAFGYDHSQIGSILAISALLYGIGKFLLGSVSDRSNVRVFMPVG
                     LILTALCNFAFGATQSYAIHFWLWALNGFIQGAGWPPCGRSIGHWFSVSERGTIFSVW
                     NIAHNVGGGVAGMLAAYCAKHFGLHNAFFIPGAIAILCALYLFWQLRDTPQSVGLPPV
                     EVYKNDYPPQTHEHIEKELSTKTLFVDYILKNKLLWVVAIANFFVYIARYSMLDWGPT
                     FLREIKGANITQGGFSIMVLEFAGIPSTILFGWISDKLQGRRGMVSMLCMIPVVLAFF
                     AIVVYPQPSLWFYYAMLSVIGFFVYPPVMMLGVMGLDLTNKKAVGTAAGFIGMFGYLG
                     RTVQAKGFGFILDYFATTYDIVTRWNIVLYTIVACTVISIVLLAFTWKVKPQ"
     gene            37392..38003
                     /locus_tag="PLJ75_01480"
     CDS             37392..38003
                     /locus_tag="PLJ75_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD03921.1"
                     /translation="MKITKKRSDGLKRQSEIMMIALELFSKKGYHNTKLEDILKKADI
                     AKGTFYLHFNGKDDLLHMIVDSQLEEMYRILSVLDISMDKPLTEVINIYRTIAQYLIR
                     DPKIKTFARIMLKEAMGLDKTLLQKLNDFYNKIIAMSADYIAKAQGKGRVFPMLDPYF
                     TSMCIVGSIKELVFQWSVNDESLDLEKAIMTAADIYFRGMVKG"
     gene            38106..38465
                     /locus_tag="PLJ75_01485"
     CDS             38106..38465
                     /locus_tag="PLJ75_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPD03922.1"
                     /translation="MKLSEIKKILDADVIVGEEMLNTVDVETAFASDLLSDVLAYAKE
                     RALFITGLTNPQVVRTVEVLDLIGVVFVRGKVPQMDTVELAKRKNIPLLRTKCIMFET
                     CGRLYGAGMKASIEKVD"
     gene            38478..39275
                     /locus_tag="PLJ75_01490"
     CDS             38478..39275
                     /locus_tag="PLJ75_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03923.1"
                     /translation="MQCANHIFSYTLKGGDFDNAGRISTSLKRELRTREYPSHMIQRV
                     AIVLYEAEINVVSYTTVGYFFAYCRPNSVHLVVKDKGKGIENIRLAVQEGFSTATDEI
                     RRLGFGAGMGLSNIKRNANVMRICSRPGEGTKICIDITSSNHYGDLIMDITVNEIQQK
                     TNFELLTTKADCTKGITGGYVGDMLSDVNANGKKGNIWITILTHPNAVAVASLKDFSA
                     IVVAGGIKPRDEFVKRADEEDIPVMYTDLSAYEVVVLLSSLGITANH"
     gene            complement(39305..40024)
                     /locus_tag="PLJ75_01495"
     CDS             complement(39305..40024)
                     /locus_tag="PLJ75_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HPD03924.1"
                     /translation="MNINQNYYERFSRTSLLIGKENLEKLFTARVFIAGLGGVGSYAF
                     EALVRAGVGTVHIIDFDTVEISNCNRQLLALGSTIGIPKVDVAEKRAKDINPDGMINK
                     NKIRITPDNVHGLIPHDIDFAVDAIDDVDAKTSLITTLLGRHIPFISCMGAANACNPE
                     SLRVSDISKTQYCPLARTMRKRLKNIGIAGGFMCVYFHEERTGSTTNVSTNNTRGSLS
                     YMPGIMGLAAAGYAINFLIGR"
     gene            complement(40195..40701)
                     /locus_tag="PLJ75_01500"
     CDS             complement(40195..40701)
                     /locus_tag="PLJ75_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03925.1"
                     /translation="MDFETTTCISCKHLEILDSYCKKLDVSLRTCIVYMILYAAKKEK
                     KKAIAFKRICYRKRDKDNPWKRVHLVLYQSEYEFCLDVKKLWKMSLANIIAFCVENVL
                     VEFFEYFSRRLKEINSDNYPYNLTSYYENRSYTFDFHKEKGIHCLKFYWGPPPEVIEF
                     NKKQKKLL"
     gene            complement(40762..41529)
                     /locus_tag="PLJ75_01505"
     CDS             complement(40762..41529)
                     /locus_tag="PLJ75_01505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD03926.1"
                     /translation="MRLVDVHCHLESQHFSGRLDAIIDAARKVGVIAFITCAIVPDEW
                     ELSQTISHSFHEVYFAAGVHPWYIKNQYFEELQKLYAITDSKYIAIGEIGLDAKTDMD
                     LAVQKKFFEEQLAIANDLNKPVIVHCRGAFNELIASLKRIGKINAGGVIHSYSGNADI
                     ARQLIRYGFYFSFGGALTYRNSKKKLQVLHHIYPDHVLLETDSPDIPPVEKSGQINLP
                     ENIIYVVKAMSELIGNNEEEIAEATTQNAKKLLNLNI"
     gene            complement(41531..41950)
                     /locus_tag="PLJ75_01510"
     CDS             complement(41531..41950)
                     /locus_tag="PLJ75_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014964235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPD03927.1"
                     /translation="MKSMTEYLTFNTRTKRDYINITTMVEDVVTRSGIKEGLCLVNAM
                     HITASVHINDDEKGLIHDIDDFLETLAPHEPISKYRHNRTGEDNGDAHIKRSIMGREV
                     VVAVTNGKLDFGPWEQIFYAEFDGQRPKRVLIKVIGE"
     gene            42193..42501
                     /locus_tag="PLJ75_01515"
     CDS             42193..42501
                     /locus_tag="PLJ75_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03928.1"
                     /translation="MKRFVILFTILIAVFIAAAIYSTNRVETEPAYSASPVDCGWDAV
                     KPHFVNDSPCPGAVYCSYGNCGEYCCPWGYFYSNPCTCRCYRTSYDAGQDCNSYFRCN
                     "
     gene            42813..42885
                     /locus_tag="PLJ75_01520"
     tRNA            42813..42885
                     /locus_tag="PLJ75_01520"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42846..42848,aa:Asn,seq:gtt)
     gene            43284..43874
                     /locus_tag="PLJ75_01525"
     CDS             43284..43874
                     /locus_tag="PLJ75_01525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD03929.1"
                     /translation="MKNAKSFQDIKYTEKENKKKIIIDAAERVFATKPFNQVTMRDIA
                     KEAGISAGAIYRYFPDQQALFVEAFLLGAEDIINEMEIIIATSKNPLKDTIYTFLSYL
                     NKHDPYFRMMTHFMLDANLSPDLINKLNEVERRVLDQFTKLFASRTNARLLAHSLFAS
                     LNGVMITFHNYPGRSKKEVRYHMNRLGETLYQLFSC"
     gene            complement(43860..45464)
                     /locus_tag="PLJ75_01530"
     CDS             complement(43860..45464)
                     /locus_tag="PLJ75_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD03930.1"
                     /translation="MALNNTYSFDEFLNWRNNVNFYADDPFLQKVAMHFAGNDFPQLD
                     AEARRLSKLASFSWRDISDDIAWPHNRIFMQHYDGHNHRIDRIVRPKALEEMEKQVFS
                     QGLFSSKTSPWERLIKMILIYENGEAGIACPLVCTEGLVALLERYHDHPELEKILQHC
                     KEGMDGNFAIGAQYLSEIHGGSDVAANDLEAVEEGDHWRLYGKKFFCSATHADYVVIT
                     ARPKGSEHVGLFVMPSWLDKQNEKRNNYTIDRLKWKLGTAELPTAEITFNGTIAYKTG
                     AVQRGVANVVGIVLTHSRLTVGLSAAAAMIRAAREATAYANFRTAFGFPINQFPLLKR
                     QLNEINLYAKRTLAGAFKIYEMFQSLPGGLKGGLTTDEDIATQQKRFRVRILIMLQKI
                     IASWDATDVLRKAISVFGGHGVMEDFSSLPRLFRDMMINELWEGPRNVLLTQIYRDMQ
                     RAASWYPLNQFINDLLYNADSCVIDEVTAAFDRCLSSNFIYPGQDNAELCLLWEEAAH
                     KLLHEFQNCALHEVEAAREDAALAGK"
     gene            45737..46591
                     /locus_tag="PLJ75_01535"
     CDS             45737..46591
                     /locus_tag="PLJ75_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03931.1"
                     /translation="MEFKICCYGYAIMTASSIEEILAIIAERFAKPFVVDDATIHYAE
                     STLGVDQHAIMDYLNDDEYGMMDLIVYPELSLRKKIEGYIPRHGFTSHQVEQLIERLA
                     SAIPCVAINVHNTTVDCYSAMCMRSYVHKLFLTKSNIPLDAIPAMYYNRYIAARVAIR
                     LYNTDSNLDVLHRLAVGLASDYNEDYIFDSIQVLISVAGNAGNIFDCMALYKYTVQKQ
                     ILAMHEFHELMKSYSMEYLMSMRYNPPMIDAAKLEYQIRLIDSICMVLFGKPAPGYVP
                     DFNDTWND"
     gene            46898..50749
                     /gene="cas12a"
                     /locus_tag="PLJ75_01540"
     CDS             46898..50749
                     /gene="cas12a"
                     /locus_tag="PLJ75_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas12a/Cpf1"
                     /protein_id="HPD03932.1"
                     /translation="MSNMQQYDNFINHYAIQKTLRFELQPIGKTREHIQKNGIIEHDE
                     ALEQKYQIVKKIIDRFHRKHIDEALSLADFSKDTAMLKRFEELYWKKNKNENEKNEFV
                     KIQSDLRKRVVSFLEGKVEGDARFAKVQQRYGILFDAKIFKDKEFISTACDDIEKDAI
                     EAFKRFATYFTGFHENRKNMYSADEESTAIAYRVINENLPRFLENKARFEKIQHTVDS
                     KTLNEIATELKPVLEKNKLETIFTLNYFQNTLSQAGITYYNTILGGKTKENGEKVQGL
                     NEIINLFNQKNKDTMLPLLKPLYKQILSEEYSTSFTISAFEKDNDVLQAIGSFCNDCI
                     FYAKNNVNGKAYNLLQTVQAFCNSIDTYNDNRLDGLHIERKNLATLSHQVYGEWNILR
                     DALQIHYEAYEQKDNGNNNNYLESKTFSWKALKDALTTYKSLVEEAQDIDENGFIAYF
                     KDMKFKEEIDGKTTSIDLIENIQTRYKSIETILQEDRNNKNNLHQEKEKVATIKGFLD
                     SVKYLQWFLNLMYIASPVDDKDYDFYNELEMYHDTLLPLTTLYNKVRNYMTRKPYSVE
                     KFKLTFEKSTLLDGWDKNKERANLGVILRKGNNYYLGIMNKKYNDIFDSIPGLTTTDY
                     CEKMNYKLLPGPNKMLPKVFFSKKGVQFYKPSQEIIRLYNNKEFKKGDTFNKNSLHKL
                     INFYKESIAKTEDWSVFQFKFKNTNDYADISQFYKDVERQGYKISFDKIDWEYILLLV
                     DEGKLFLFKIYNKDFSPYSKGKPNLHTIYWKNIFSHDNLNNVVYKLNGEAEVFYRKKS
                     IEYPEEILQKGHHVNELKDKFKYPIIKDKRYAEDKFLFHVPITMNFLSKGEPNINQRV
                     QQYIASTSEDYHIIGIDRGERNLLYLSLIDATGKIIKQLSLNTIKNENFNTTIDYHAK
                     LDEKEKKREEARKNWDVIENIKELKEGYLSQVVHQIAKLMVEYKAILVMEDLNTGFKR
                     GRFKVEKQVYQKFEKMMIDKLNYLVLKDRQATQPGGSLKAYQLASSLESFKKLGKQCG
                     MIFYVPAVYTSKIDPTTGFYNFLRVDVSTLNSAHSFFNRFNAIVYNNEQDYFEFHCTY
                     KNFVSEPSLQKNVKSSKMHEYNNLKDTTWVLCSTHHERYKKFKNKSGYFEYKPVNVTQ
                     SLKQLFDEAGIDYQAGADLKEAIVTGKNTKLLKGLGEQLNILLAMRYNNGKHGNEEKD
                     YIVSPVKNNYGKFFCTLDGDASLPVDADANGAYAIALKGLMLVERMKSNKDIKGRIDY
                     FISNNEWFNYLIAKNTLNKSK"
     gene            50768..51328
                     /gene="cas4"
                     /locus_tag="PLJ75_01545"
     CDS             50768..51328
                     /gene="cas4"
                     /locus_tag="PLJ75_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HPD03933.1"
                     /translation="MEPYIPISMLNDFIFCPRSIYFHQVHGGMEQDMYHTEVQIEGKA
                     AHQSIEKKYYSTQKDVLIGIDVYSEKYNVAGKIDIFDVKACKLVERKNKVVTIYDGYV
                     FQVYAQTFALREMGYEVKKIVIHDKTHNKNYPIPLPEDDDAMLNKFEKLIDDVNSFDL
                     HDTGFNANIEKCKRCIYSHLCDYCLC"
     gene            51322..52287
                     /gene="cas1"
                     /locus_tag="PLJ75_01550"
     CDS             51322..52287
                     /gene="cas1"
                     /locus_tag="PLJ75_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated endonuclease Cas1"
                     /protein_id="HPD03934.1"
                     /translation="MLSLPDFEEKSIIVCFTTEGQTVSFKNDNVVIKDKKGKTILQHS
                     CYRLFSLWIVGHTTITSGILQRSKKFGFSIYLFSYSFKITGVWNSCAEGNFLLREKQY
                     SYNNLDIAWHIVTNKIKNQIALLKSIRDKEEEQKSSIANLDVYLHHTVEPLDLKMILG
                     IEGITTKLFFKEWFTNIAWNGRKPRTKIDTTNTLLDIGYTFLFNIMDAMLNLYGFDVY
                     RGVYHRNFYQRKSLVCDMVEPFRCIIDKKIKNAYNLGQIKAADFTESRGQYFLNHDKN
                     KEYTGWLLQEIMKYKKDMFVYVQKYYRAFMKEKPIKDYPEFLIKE"
     gene            52295..52567
                     /gene="cas2"
                     /locus_tag="PLJ75_01555"
     CDS             52295..52567
                     /gene="cas2"
                     /locus_tag="PLJ75_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPD03935.1"
                     /translation="MLLVSYDISNTKLRTKFSKFLEKYGERIQYSVFEIKNSERVLKN
                     IETEIKQYFEKKFTQSDSILIFHLSEKCTITRYGYAKNRESDILIV"
     repeat_region   52723..53521
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=52723..52758
                     /rpt_unit_seq="gtctaaaggtattataaaatttctactattgtagat"
     assembly_gap    53541..53640
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     repeat_region   53651..54319
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=53651..53686
                     /rpt_unit_seq="gtctaaaggtattataaaatttctactattgtagat"
     gene            complement(54516..54830)
                     /locus_tag="PLJ75_01560"
     CDS             complement(54516..54830)
                     /locus_tag="PLJ75_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03936.1"
                     /translation="MTDLEYLINIMDNVEDPDGDHEPGYIRQWPMIRDDLENTDIVKE
                     QDIRYITNGAFFAEWVNEEYMYVENHEEWYWHIAEIVKGAYPLEKLPMHVRDSAYKLY
                     YK"
     gene            complement(54956..55489)
                     /locus_tag="PLJ75_01565"
     CDS             complement(54956..55489)
                     /locus_tag="PLJ75_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD03937.1"
                     /translation="MKYYESLEKFKTYNIVLLCGLYGAGKTEFASTYFKGSGLYRVSR
                     LELRKLMYEMTHFGESWEAEKFNEEDDVLAKHVERKVVEHFLQIKRNVLIINTFMTKK
                     SRQRFVNIAKETKKTIGAIFLDTPLDVCIAQSKRLNTAIPENVIRQLHSRKELPTKLE
                     GYNDVLIINDFSLSSNG"
     gene            complement(55647..56579)
                     /locus_tag="PLJ75_01570"
     CDS             complement(55647..56579)
                     /locus_tag="PLJ75_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD03938.1"
                     /translation="MNDTKLWMALSRVQGVGIVALKQIYTALSNLNISLYDIVELSPK
                     TMAFETGLPIELCQLVANVSSTIGTAQKEYEQLLDNTIEPILFFDVRYPQRLLRSMEY
                     PPAILYMYGNYDVITSKQIAILSDHTASERGHYIAYQAAHIAAQHSITVTSGMAKGPQ
                     SMAHRGAIEGGGNTIAVLPMGLLHLSIPQILQDCVDLSRWLFISPFEPAQEYAIHHSY
                     KRNSLIAAMANALIIIETTPDGGSFEAAKSAHNYKTPLFVVEYAEYPATASGNPGIIK
                     EYNATPLKGRMQNGTLLPNMDMCIAKARYGDSHS"
     gene            complement(57183..57308)
                     /locus_tag="PLJ75_01575"
     CDS             complement(57183..57308)
                     /locus_tag="PLJ75_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03939.1"
                     /translation="MRAHDRAACRRQPLHDIVDKAGEEARCAPAVERIYPEGAFL"
     gene            57535..>57754
                     /locus_tag="PLJ75_01580"
     CDS             57535..>57754
                     /locus_tag="PLJ75_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03940.1"
                     /translation="MGKKTILVALLGILMVAGSIPQTATQAQQEKKKIYISGITVEGA
                     PASMAREIRNIIEVTLYEYGSQYRVITDD"
BASE COUNT        18017 a        10033 c        11491 g        17813 t
ORIGIN      
        1 ttcacgtaat aaaatttttg cggctgcaag gcttgcccct tttgcaagct gaacattgcg
       61 aatatcctgt tgattgatgg taatgatatt tagaatgttc agggctttca tggtttcaat
      121 gtgctcaagt ttaatgtgct ctgatagcgg ttgtggtata gcagtcttta tggcaccact
      181 tttttcaata acaccattcc tgagaagaat agcaatgaga tcaataagtg cagtgccgct
      241 tatccctgat ggtttgccat cacctaacga agtataggtt aatgtattat tgtaaagagt
      301 aaagtgggtg atagctccct cggttgcagt cattccactt gatatattca ttccctccaa
      361 ggctggcccc atagcacatg atgtagcaac aatagtgccg tcttctttta taattgccat
      421 ctcaccgttt gtgccaatgt caaagaaaaa gctgttgcct ttcgtttttg gaagtgtacc
      481 aacaccacct gaaaagtcgc cgcccagaaa tgcactgact gctggtaaaa cggttatttg
      541 cgcgttagga aatgtttttt ttaaaggggg ataatggtta tagctgtcaa aagaagtgat
      601 gagtgtggta tagggatggg tacctaaagg tgtgatatca atccctaaaa aggaatagct
      661 catcacggtg ttaccactga tggcgatagt tactatggag tgtatatcaa tacctgaaat
      721 gttgcataca tcctgaatag tggttattat tgtttgtaaa agaatctgtg taagttgact
      781 ggctttagca gggtcatttg cattgcttat tcgcgtcata acatcatgac caaatcgtcg
      841 ttgtggatta aggagagtaa tttcgggcat tgcttgcttg gttgtcatat caacaaaagc
      901 tatctgcact gtggtggtac caatgtcaat tgccacggcg taggtgtggg ttgggttaag
      961 ctctggatag cgaggacgct gtgtaatgct gtcaatccag atatatgcat cgcctataat
     1021 tacagcacgg caggctaatc tcaaggaact atcgcccaga cttttttcat aatcatccgg
     1081 ctgggataat aaccctgaag cgtgtaccgt gcattttttg catatacctt ttccaccgca
     1141 tggcgtgtgg agtataatat tgcaagcaag caatgcatcg gctatggtag tgccagcggg
     1201 tactgttaca ctccggtttt cagggaggat ggtaattgtt acacgattgt tttgcattgc
     1261 taataacttt atattcaatt atagcaatat atacttgcta ttattgaaaa gtaaatattt
     1321 tattattttt ttctttacag atgaaatgtc cttgctattg aattgccctg ttgttttaat
     1381 cgtcatgaac ttttgttata ggatgcactg tgcacatgga tgcaacaatt atttcatatc
     1441 ctgatacctc ggttgtacaa ttactttcgc ttaccgaaaa cagatcaaaa tacatgctgc
     1501 catttgctgg taaatttaga gtggttgatt ttgtcattgg caatgcaaaa gaggttgatg
     1561 cacataccat catttacagc aatgtcaatg atgatcttgc caattatgta cagcttcatg
     1621 gtattgatga atcaaggctc aaggttgtgg ttgataattt taacagtgtt gatttttttt
     1681 attcaattgt tcgtgatagc gattcagcat atttcataat atataatggt gataatcccg
     1741 gtattataaa tttttcgcat cttattgaac gtttcattac cataaaaaaa ccttcggtac
     1801 tgtataagat gcattttgaa gaaaaggcaa ggatggcgca tcgcatcctt gttaccacta
     1861 aaaaggcttt gcttgatacc atacgaaaag cattaaagca taatcatagc gctcccaaca
     1921 tctttgaaat gattattaat atgatggtta atatagggct tgcgaaaggt gaattttcat
     1981 gtatgtactg gcccttcagc acaatcgttg attattataa tcacaatttt gatgttatca
     2041 ataatgctga ttattcaaaa catgtgtttg atgggcacat aaacgcaaag cttgagctgt
     2101 accgttattc catgcttggt cccgatgcaa ggatatcaca ttcatttttg tcagatagta
     2161 cctatataaa tggcgaggtt gttaattcta tcatatttcc gggtgttgaa attgggaagg
     2221 atgctgttgt ccgtgattcc attattttgc cttataacaa gattggatca aaggcgcgta
     2281 tcatgcgtac aatcattgat gaacgtaccg tagagccttt tagtgccgat ataataaaca
     2341 tagggaataa ctgcaagatt ggtacaaccg agatagggat gaaaaacagt gattatccca
     2401 gggtgctgta taacagtatt acccttattg gtaaaaattg catgatccct gacggtataa
     2461 cggtgggtgg ggcatgctat attgcatcag gcaggacaca gcaggacttt gcaaaacgaa
     2521 aatatatcta taatggcacc tcggtgtgat atgtttttcc cttattccga tgaaaatccc
     2581 actcgctcat tttcgctttt tactgttatt ctgatacttg caaattgtgt catctattat
     2641 atggaggttt atggacaacc tgcatttgca caactcatca acagctatgc gcttgtcccc
     2701 tgtaatgtga acaagttaca tcttgatgac tatcgctatt atgttccttt tataagtcat
     2761 atgtttttgc atgttaatat tattcatgtg gcaggcaaca tgcttttttt gtggatattt
     2821 ggtaataatg ttgaggatgt gctgggaggc atacgcttct atgcttttta tgtattatgc
     2881 gggcttgctg ctgctttaac attttattta tttcatcata ctgaatgtac accaatcata
     2941 ggagcatcag gtgccgtgtc gggggtactg ggtgggtatg tggtactgtt cccatcggcg
     3001 cgtgtcaagg tgtggtttat attttttgtg ctgcgattgc gtgcatggtt ttttatactg
     3061 ttgtggtttt tactacagtt gagcaacctt tacggtgagc agtctggagt aagtaatgtg
     3121 gcatggtctg ctcatgttgg tggatttatt gctgggtttg tgctcattat gttttttaag
     3181 aagagatatt gatggtgtat ttatggagaa ggtaacgata taaatactaa tcatgaaaat
     3241 gtataataaa atagaaaaat acctgtgtgt tgcggtattc ctgttgttta gcatacgctg
     3301 gttgccagtg tatgccgatg ttgctgtgat cccctataaa gtagagagtg ctgatacctc
     3361 gtttaccgag gctatgggga gtgaatatgc aaagcttgtt gctcttgcta tgtatatcca
     3421 gaaaggcata agtatctact cgcacgattt gctggaaaag gacatgcatg aattttccat
     3481 tgatcctcag ggcgtggtgg caggctacaa attggatacc ctgggtaaag cccgctctat
     3541 tgatcgcata cagattggta cgctaaccaa aaccagtaaa ggctttaccg tgaaaagctt
     3601 actgtatgat gttgcaacgg ggaagatcgt ttcgcgctgc aatgaatatg ccgatacgct
     3661 gtatgatgta gccattgccg agatgcgctc gttgtacctt tcctatcccg acaacaatcg
     3721 tgccatgcag caatcaatct acgatattgc gtttgttgtt gataattcgt acagcgcttc
     3781 ccgcgagtgg aaaagcatta agcagggtat tgtcgctctg tgcgatacaa tcagcgataa
     3841 ctggaatgac atgcgcgtat atgtggtgcc gtttttgacg caatcgaaaa agattggaac
     3901 ctatgctgtt acaggcgcta ctactctgga tgatgtttta caggagctat cgcttgcaaa
     3961 aggaattgtg acaggcatta acccgtgttt gcagtatatc gcaaaggggc tgccatggcg
     4021 caatgatgca aaaaagctct gtattgttgt tgcagcttca aggtgcgatt acagcaatgc
     4081 acgttcactt cgttacctta tcaagaaaaa gggcataacg ctgtatgtga ttggcacagg
     4141 aggtttaacc catactgagc gcacagcgtt atcgtttatt ggcgatagtt actatgatgt
     4201 gacatacagg cagaggatgt atgatgttta tggaaatcca gttgatgtgt tttgcgaagc
     4261 tggaaggata ttccatggtg atgcaggtga ccgatggaag gatggtgtga ccgctacaaa
     4321 gacgaaggca gcaaaacctt ttgttgccga ggtgtttggt gtacagaatg ctctgccgta
     4381 tgaactgcct gcgctgtatc caaagcaaac agcaataaaa atactcaatg cagaagagtt
     4441 ggaaaataat atcgttgata tatgccagac tattgcacat aacaataaac tgactggtca
     4501 ggtaattgcc cgtgttctta ttgctgatgg cggatactcg ctatggctgc cggttgcgga
     4561 taaaagaatc ctcaactatt tagcagagaa tcaacacatg cgcatgtatg ttggcatttc
     4621 tccaaaacag gatttaggtg caccctatgg ggtgggactt ttaccggttg cagtagttgg
     4681 gctgccgggt agtcatattc catcaatgct aaaattaacg gtgaaggata ttattcagcg
     4741 cccgcaatgg aaaggatttt ttaatcctcc cctatggttt attcctgtta cggtaaagca
     4801 gattatacca ttgtcgggag aagaagatat ccgaaataaa taatacttga cgattggaaa
     4861 taaattgagt atattaatat aaaatataat ttcctgtctg ttatagaact cgcattagca
     4921 ctctcctgtt ttattatttt atggttattg tattcatgta ttaatggtat tgtaatttta
     4981 ttattttata ttaatggagg tagatttacc tatgcgtatg gataaactga cattaaagac
     5041 acagaatgca ctgtatgaag cgcagaatgt ggcaacacaa tataaccacc ctgaaataac
     5101 ccccgagcat gtattgtatg cacttgtcaa tcaggatgat ggcatattta cgcctatagc
     5161 tcaacggctg ggtgcttcgg tagatgaaat taagcgtgaa accgagatgg cactttcggc
     5221 aatgccaaag cagtacggcg gtcctggcgg cggtgttatg tcatcagttt tacgtgatgt
     5281 gctgaatact gcatggaatg aagcagttac attaaaggat gaatatttaa gcaccgagca
     5341 tcttatactg gggattgcca caacaggaaa aactaaagcc cgtgacatat tgaatgcaca
     5401 tggttttacc aaagagaata tcctgcgtgt gcttgccgat atcagaggca ataaacgtgt
     5461 taccgatgag tcggctgagg ataaatataa tgcgctggaa cggtttggca gggatttaac
     5521 caaattggca cggcagaaca aacttgatcc tgttgttggc agagatgagg agattcgtcg
     5581 tgttatgcag gtactgtcac gcagaacaaa aaacaatcct gtccttattg gcgagccggg
     5641 tgttggcaaa actgccattg ttgaaggctt ggcacagcgt attgttgctg gtgatgttcc
     5701 cgaaagcttg aagaataaac gcattgttgc gcttgatatg ggcgcattga ttgccggtgc
     5761 taaataccgt ggcgagtttg aggaacgctt gaaggctgtt attgaggagg ttgaacaatc
     5821 cgaaggtgat gttattttgt ttattgatga gttgcatgtg ctggttggtg caggcgctgc
     5881 agaaggttcg gttgatgcat cgaatatgct taaacctgca ttggcacggg gagagctgcg
     5941 ctgcattggt gcaacaaccc tgaatgaata tcaaaagtat attgaaaaag acaaagcgct
     6001 ggaacgacgc ttccagcctg tatttaccag tgagccctca gttgaggata ctatcgccat
     6061 tttgcgtggt ttaaaagaac gctatgaggt acatcatggc gtgcgtattt cggactcagc
     6121 ggtggttgct gcagcggtgc tgtctaaccg ttatatcact gatagatttt tgcctgataa
     6181 ggcagttgat ttaattgatg aagctgcttc gctcataaag atggagattg attccatgcc
     6241 aatagagctt gatgagattg aacgtaagat acgccagctt accattgagc gtgaagcagt
     6301 aaaaaaggaa aaggacaaag catcattaga gcgtcttgcg aagattgaag aggatttagc
     6361 aaatttaaat gaaaagaaga aggaactttc ggtacagtgg caaaatgaaa agaagatcat
     6421 agtagaaata cggaagatta aagaagagat tgaaaattta cgaatacagg agcaaaaagc
     6481 cgagcgtgaa ggcaatctga atttagttgc tgagatacga tatggaaaga ttattgagct
     6541 tgaaaagaag ttaaaagcgc tcaatgataa gttgaatgat attcagaaag atcgaaagct
     6601 tttaaaagaa gaggttaccg agcaggagat agctgctgtt gtttccagat ggaccggcat
     6661 acccgtaagt aaaatgcttg aaggtgaaaa agaaaagctt ttaaaaatgg aggagattat
     6721 tgaaaagagg gtggttggtc aggagcaggc aatacaaact attgctgatg cagtacgccg
     6781 ttctcgttct ggattgcagg atcccaacag acccattggt tcgttcatat ttttaggacc
     6841 aacaggtgta ggaaaaaccg aaacggcaaa agctgtggca gagtttttgt tcaatgatga
     6901 aaaggcgata gtgcgtattg atatgtccga gtatatggaa aaacattcgg tagcacgcct
     6961 tattggtgca ccacctggct atgttgggta cgaagagggc ggacagctta ccgaagccat
     7021 ccgcaggcgt ccctattcgg ttgtcctgtt tgatgagatt gaaaaggcac atccggatgt
     7081 gtttaatgta tttttacaaa tactggatga aggcaggctt accgatgcaa aagggcgcac
     7141 agtagacttt aagaataccc ttataatcat gacatccaac attgggacag catatatttc
     7201 tgaccctgcc ataccctatg aggaacgggt aaaaggggtg cagcaggagc tgaagatgca
     7261 ttttaagcct gagtttttaa accgcattga tgatgtgatt gtctttaacc cgttgacccg
     7321 agagcatatc aataaaatta tcatgataca gctcaatgaa gtgggcaaac gtttaaaaga
     7381 acgcggtata aaacttgagc ttacacaaaa ggcgttagat taccttgtta acgttgggtt
     7441 tgatccacag tttggtgcac gtccgttgaa gagggcttta cagagatatc tgctcaatcc
     7501 actttccgta aaaattttag gcggcgagat aatggatgat gataccgtta ttgtggatta
     7561 caagaataat gaaatccaat ttaatgtaaa acgcagtaaa gctgagaagc aagcagtgta
     7621 agcatgtaag caataagggg atgtccatgc atccccttat ttttgtttaa ctttagatcc
     7681 tgaatcagag ttcagaatga catgccaata catggtcatt cagaataaca cagtttcgaa
     7741 gaatctctcc cctcacccaa aacattataa tactatagat atttatcatg aggttaaaat
     7801 cgtaacattt taaaatgctg tttaattatc gtttatgtaa tagtattgac aggcataggg
     7861 ctataccagt aatttattat tattacatgc agagaggctg ttgctatgga atatgaaatc
     7921 tacgatgtga ataacatact gttgccggtt gagcataaaa ttggtgcact gagccgtgct
     7981 aaaaacctgg ttgccgagat gactcatccc aacattgact ggaaatacat ggttaccgag
     8041 atcagagctt atctgtatga ttacatgtat gacattgtcc ctcacagtga tagggtgctg
     8101 cccgttatat tccattatct taaagaagca acggtacgca agcgtggttc aacaatcaga
     8161 gctgctgata catttctgga tcgctatctg tttttactaa aaaaagaaat tgcgggcgat
     8221 agttccctgg acaatgttac tgccatcttt gataaagaag caattgattt ttgcaggatt
     8281 ttactttcgg atactcagga aggattttat ctggaggatg ttaatcaacg tattatacaa
     8341 ctcatggagc tatcgcttca gcgaaagaaa ctggatgcac agctatttga actgtgcaca
     8401 gatatcatca tcaatcaatt taaactgtat gtagaacgaa gtattgtggt tgaagaagaa
     8461 tatatggact cattgcgaaa cctgtggaat agtgatggtg aatcaatagc aacactacgc
     8521 cagtatgttc aaacagtcac aaaagaagcg tatgaaaaaa aattacagat agcactggat
     8581 agccgaacta ccatcacgga ttatgaaaaa ctgttacagc aattaatcaa tgttattgat
     8641 tttcatcata atgaaactgc atgggaaaaa atatgcattg cagcccgtga atgcattgtg
     8701 cgcaatataa ttcaggatga cagcgtgctg ctttctttac tatcgtttat ggtaaaaaaa
     8761 tcgcagcagg gtaacgatac caatctccag ctttatatat cgcgcagtgt agcttcactg
     8821 tgtggtgtgc ttgcacagca gcagcgtttt gatttgctaa aacaggttgt acagatggtg
     8881 gtgccggtgc ttatatccga gattgaacga ggtggtaatt acaatgctgc atttgcaaca
     8941 atctatacta tcggtaaaac tgttgtggaa atcaataacc gccccatcat tgaccttttt
     9001 gttgatatac ttgttcatgc gcgattttgc tttccacaat ttacaggtat tgcttcagac
     9061 tggtcggtta tggtgaatgc cagtcatctt gccaatatcc gcacatggct tgaacttatt
     9121 gaacttaatc ctgtgtacat gaaacgcctt gctgcaagcc ttattgccaa ccttacactg
     9181 gggggtgttt ttttaaaaga cactgatgta ttccagcgtg atatatcacg gttgctgaac
     9241 agcaactata aagatgtttt ttaccttatc atatcactgg ctgcagtctt cccggcattt
     9301 tatcatgaca tcggcgctac cggcaatata cgtgccttta ccgaacgtgt tgataccaat
     9361 caccagatga acgatctcat ccattttgtg cgcaagcagg tgcatgttga gtcaagcagc
     9421 cggacggttg cattactcca gcgagtcatg gacttctggt tgaccggtga caaggaatta
     9481 ctcaaaggta tggtgccgtt agaagtatat aacaatttag acagaacatt caggcttatc
     9541 aaccttgata atgaatcaat tgcacggcgt atttacatgg aaacacgcca ttattttccc
     9601 gaatatgtaa acgaaaaatt ctgggatttt ttctacaagg ttggcaaaaa acgttttatg
     9661 gatgctattg ctcaccatac ctttgatggg atggatgaaa atgaaaaaaa ggatgctctt
     9721 gattgtatag tagaatattt tgataaacaa tttcctgctg aaatgaccaa gatgttgcac
     9781 catattgccg gaatgtttga tattgataca tcgcgcaaac agatatggcg ctttctctat
     9841 gaaattccgg atgatgattt cagaaaactg tttgaggatg tacaaaggtt agatgtttct
     9901 accgtgaata tagagaaatt tattacattt ctgcatgtgt accgcatgat ttttgataag
     9961 tataatttct ctgatattag agctattgaa aaactgcatc aatatgcatc tgaaaatctt
    10021 ttcaacccac ctgatgactt tttccagaaa cttgaaggca atgatttgtt tcaatcgctt
    10081 caggcaattc ttgatctgca aaacagatta aaatgggaca tcctgttgtc tcaggatgtg
    10141 tttgaacctg tggatacaat tgagtttaaa cgacatattg cttttggcat accatcaatg
    10201 tatggatcat ataaagaaaa aaagtttgat acattaaagg tatttttcca ctgcaatatt
    10261 gtgcgggtac tcctttttga aaagattatt gaaaatataa gcatctatcc tcatcaaaaa
    10321 attgactatg atgcaatcaa gcgagtggta gaacttttta tccaatcgtt tgaaattgat
    10381 ggacttgcca atcacgagat gcgttcggtt accagcttgc ttgatgcacc taatattacc
    10441 gtaacgcagt ttcgtgacat agtaaatagc cttcttgtta ttcatggtga gatctctgac
    10501 aggtttaatg atacctttaa gtcagtcagc cgtattgcta ttaaaaatat aggaatggat
    10561 aatataatcc ctgatttcat acctcccgat caacctgcta atgtcgaggt gattgttgac
    10621 aggtttttgc gtcacagggt tatgcagtca ccattattgc agttaatgga taacctttta
    10681 ttaaaattaa gggaccatct caattcagtg tattcctata tgggaaatat tgttgtactt
    10741 ggtgcaattg atgcccgtat gaagaaggga aagcttattc atgtgattgg aaaatatgaa
    10801 ggtacttatg atgaaacaga gctctatgtt cctttgtggg aggtgggtgc caaggcacag
    10861 gggctgatta ttgctgcaaa tatggatggg attaatgtcc ccgaagggat tgttatatct
    10921 tcagagttgt ataaaaggat aaaagatggc aatataaaca atccgaggtt taaacgaaag
    10981 ctcatctata tgttaaaaaa atatattgat gcatttactg gatttagatt tgccaatccc
    11041 caaaatccca tactgttgtc tgtgcgaagc ggtgctgttt tttccatgcc gggcgtaatg
    11101 gatactatta ccaatgttgg catgaccgag gaaattgtac aatattttac acagtttgat
    11161 gaatggtttg cgtgggattg ttaccgtcga ttgatacacg attttgcaat atcagcatat
    11221 gggatggacc gtcatatctt tgaaaatctt atgacgcagg caaaggatga ggctggtgtt
    11281 gatttgaaag aaaagctcaa tggcaaacag atgtcaatgc taacccttaa gtatcgctat
    11341 gcaatcaata aagccgggca ctctgctccc aaagatcctt atgaacagct attctatgcg
    11401 attattgctg ttttcaaatc atgggattcc cctattgcaa ggaactatcg ccaatttatt
    11461 aatatttcag atgattgggg cactgctgtt attgcacagc gtatggtttt tggcaatcta
    11521 tcgccaacct ccattaccgg tgttgtgcac agtcaatata ttgaatatga agagttgcag
    11581 atagtgggcg aatataaaac aagggcacag gggcatgata ttgtcagcgg tgttgcaaag
    11641 gtatttccaa taaacgaaca gcagaaatta aaatttgccc gctttgcgat gtatccttcg
    11701 ctggaaaaag catatcccaa ccattatgct acgctacgcg atgctgttag ccggttacgc
    11761 aagcggtggg gcaatgatat cgaagttgag tttacatttg aaaatgatgt attgtatata
    11821 ttgcagatac ggggtatggc aaaacatatg tttgatatag aagagcttgt agaaactcca
    11881 cagcagctat cgcagtacct gctgggacag ggattggctg cttcaggagg tgctgtaagt
    11941 ggcagggctg tatttgatat caatcgcata gaagctatac ggatgcaata tcccaaagat
    12001 aagattattt tgatacgtcc ggaaacaaat cctgaggatg tcattggttt acaaaaatcg
    12061 gatgggattt taacctcggt gggaggcatg acatcacatg ctgtactgca gatgcgccgt
    12121 ctggaaaaat cgggcgttag tgatttttcc ataatgaaga ttgctgaaag tgagaacata
    12181 gctgttataa acagagaaca gggggggaag atagtcattc gagagggtga tgtcataacc
    12241 attgatggca acaccgggca tgtttatctg ggtgcgcatc ccacacggaa ggtacatcga
    12301 taatatcgtt atgtgcaaaa aagcttgaaa tatttataat agcttttcca atagtcactg
    12361 gaagttgatt cttaatagta gcgttattat tatttaccta aatttaaata taaaaatgga
    12421 agctggggac ggcaacatcc tgtcgggtat ataattattt tactatatat ggaggatgat
    12481 tatggcagta ttacctgatg caccaattgg tgttatggga attggacgca taggcaagct
    12541 tctggtatgg ctctatgccg caaagaagca gcaaaaagaa atagttatag caaccggaag
    12601 aaaaacgggt acctcattag aggatttagc gacgtacttt gaatatgatt ctacctatgg
    12661 ttcttttaat aggtttattg gcggtttcag tggcaagagc gcggttgaga taaaagatgg
    12721 tcatgtttac ctcaatggtg taaagctaat ctggttaaac gagcctgact acagggtgcc
    12781 cggaaagata ccatgggata catttggcgt gaatgttgtt tttgatacta cgggtaagat
    12841 gcttgatcca acgcgaaatg atgaaaattc tctgcggggt catcttaatc atgctaagaa
    12901 ggtgattgtt acagcaccat ttaaagtaaa agataaaggt aaacagattc cggatgatgc
    12961 ggttaccgta gttggcggtg ttaattttga taagtatgat gctggaaaac atgttatcat
    13021 ttccaatgct tcatgcacta ccaattgttg cgcaccacct atcaaagcgc tggttgatca
    13081 ttttggcgat aaatttattt catatgcatt aacaacggtg catgccgtaa ccaattcgca
    13141 gtcaacgctt gatgtattac ctaaagatgg cgataaagac acccgtaaac ggcgcagcat
    13201 tttgaacaac atgataccca caagcaccgg tgctgcaaag gcagttctgg aggttatacc
    13261 cgaagtgcag aaactgggta taggttcgca ggcttcatcc ataagggtgc ctgttaatac
    13321 tggttcgata gttattctgg atgtttcgct tcttggtgat tatgataacc agtatatcca
    13381 taaaatattt aaacagtact cagaaaaaaa tccagatatc atgtgttact cagagaaaca
    13441 gcttgtaagc tcggatatcg tgggaagtac ctattcaact atctatgatg cagaatttac
    13501 ccatcaccga accaccaaac gcggcgataa ttactatacc atggttgact taaacttctg
    13561 gtatgataat gagtttggtt acgtatcttc actttcacgc ttatatgatc ttgttaccgg
    13621 acggcggtaa tgcgtacttt gttttgaaca atagctatag tttcaggggc aatccttata
    13681 tgtcagggat tgtctctgaa acaataccaa tacctgtcaa aaaataaact gttgacaatg
    13741 atagtttatt ttagtatagt tatataaaaa aggggagata atttgtgaag agggtagggt
    13801 ttggattttt gatagttttg attttttata atgctactac agcttttagc aaagaggtcc
    13861 cttttaccct tgaggatagg gacaggatta tccgtctgga agcaaaatta gatgaaatag
    13921 ataaacgctt tgaacaaata gataaacgct ttgaacaaat agataaacga tttgaacagg
    13981 ttatctcttt tttatggata ttgacaacta tctttattgc aataacctcc gcaaccatag
    14041 gatttgcatt ctgggataga aaaacgtcgt taaagcctat tgaagaaaaa gttactatta
    14101 acgaaaaaaa acttgaacat gcattggcag cgttgcgtaa attggcaagt gttgataaaa
    14161 aagtgcatgc catcttgaaa gagtttaatt tattataaaa ttgattgata cattttttaa
    14221 atttaaatct catctatttt acaagatttt ttactattat cctgccattt tttagttcca
    14281 ttgaatcgat gtgggtactc cgtcaatgat aggttgatgt gtgctattgt cgtggtatgt
    14341 tttttatttg aagataccat tgcattttgt attgcgccac tcaatagtgc aatgtatcgt
    14401 cagtgcaatg aacagtgctt ttatctttcc tcttcataaa aacataatcg tatattcggc
    14461 aaaaaaactg ttactgcata gtttgctttt tttataaaaa aggttgcatt ttgaaaaact
    14521 atcggtacaa taaggattgt ttttttgaat gaaactatat actatcagga ggagccatga
    14581 aaaagtttgt catagtattt gcgttagtat tttttgtctg tgcaattgcg cagcatagta
    14641 tcagcatctc gttaaatcca aagagagttg catgcgagca gtcatgcact gaaggaaaaa
    14701 agaaatgttt tgcagatgca aaagatagcg aagttaaaaa aacagcatgt aaggttgcct
    14761 ttgacgagtg tatgcagaaa tgcgctaaag attttcaata aaataactgg tttatttttt
    14821 attgaagtaa atattccaaa caggattgaa atatttggcg atagttatcc tgaaagttga
    14881 ttcagtatct aacgttgtat tctggattcc gggtcaggcc cggaataacg gtgttgaaaa
    14941 agagatccca ggttaagccg gggatgacaa tgaggtggta tgtcattgcg aaaggagtga
    15001 agcatctctt atataatcga atagattcat tgggactgtt tcactcagaa tgacaaaaag
    15061 tgcagtgcct cttataatga caatggtgaa taacactatt ctcagaggag ttacccatgg
    15121 ccggtgtgcg gctacccata ggggatgaac attatacatc cctcaccatc aggaaataat
    15181 attccccctc gtcttgcgaa ggagaatgac cgcggtgagg ttaataatta tagttctgga
    15241 ggaaaagtta atgaagcgaa aaattgtaat cactgtatcg ttagttattt cgttgctggt
    15301 aattgtctat gctcaaaata ccaataggct aaaggaatct ggttttaaca atgcttttac
    15361 actaccacct gaaacaccat atgaacagta cgttgagttg atgaccaagg ttatagctgc
    15421 tgctcgtctt gaaactgaca ataaagaagc aattgtggct atgaatagtc cgtttgtata
    15481 taaaccggca gaaaagccag taaataatat ctattcaaag ggaatattgt taattcatgg
    15541 tttatcggat tccccctatt ttttacaggc tataggtaag tattttgcaa gcaaaggttt
    15601 tttggtttat ggactacttt tacccgggca tgggactgtt ccgggtgatt tattaaaggt
    15661 aacctatgat gagtggataa aagctaccca ttatggcatg cttcagttaa agaaaaatgc
    15721 acaagaggtt tatatgggtg gattttctac cggggcaacg ttgtcggtta actatgtgtt
    15781 gtctaacaca aatcatgatg ttaaaggctt atttttattt gcccccgctt ttgctatcaa
    15841 ttcaagcgta gcatggttag cacctattgt aagcacattt aaagattgga ctgacatcga
    15901 tgatgatgtt gactatacca agtatcagtc gttgacttta aatggagcag cacaaacata
    15961 caagctcata aaaaaggttg atagctattt tgaggaaggt aaaacaattt cggtgccggt
    16021 atttgttgtt cagagtattg atgatgaaac ggtaaaagca gagcgcacct tatatgttat
    16081 gaagaattat gttactggca aaaaaatgtt tgttttatat actacacagc ctgagaaaga
    16141 ttatgatggt gaaggcaatt ttgttgtaac ggttaacagc tttttaccaa aagagaaaat
    16201 tttaaatttt ccgcatatat gtgttaccat accgcctgat gacccccatt atggcagaaa
    16261 tggtgattat agaaattgct tgtattacaa acagggcagc cctgaaagga ataaatgttt
    16321 aaccgatccc aatatctgga aaggagaaac cacagcagag aatattgaaa agtataaggt
    16381 cattgcacgg ttaacgtata atccctattt tgatgaaatg tgccagaaga ttgttgagtt
    16441 tttaaatata attaaaaaat aatgctattg agcttctaag gtaagttgta gtatacatat
    16501 gcgaaagtat aaaattatgc ggatatagtt ttttatgatt agatagcaat gcttctaata
    16561 gattttgatt ccgggagagg tcctgaatga cggtattaaa gtcatcctga acgtgattca
    16621 ggatctcagt tgattgctga gattccggtt caagtccgga atgacagtga gttggtatat
    16681 tttcctgagc aatagtgaaa gattatgttg tttggaacag gaatttttca gttgctttgt
    16741 ttctgtaaaa tgacaatggt gcataattct atctccaatg cttcagtatg aggctaccca
    16801 tagaggttca ccacttactt ttttaagcac gccacaaagc cccgaagatg ttgcgaaagc
    16861 agttgtaaaa gctattacaa caaaaaagcc tgaaatagtg gttcctgctt cgcnnnnnnn
    16921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaatagt ccaattatac ttaatttcta
    17041 aaaatatatg ctataattac tctatatagt tttaacggta tggagggtgc tcattgacta
    17101 atacggttaa tggcaataaa tcttttttta tttcaaagca attataacat catgaacgag
    17161 gcaattatgg attatctaaa actttaccga cgattttatc tggatatgtt gatccatggc
    17221 aatgcgctat ctgcaattat tggtgcaccg cttacggtat tattctttat tttacaaaca
    17281 gaaataacac tttccaacct tcatgtggtg gtagtgccgg gtattattgt ttttgttgtt
    17341 gttacattaa tgacgtatgt ctggtatcag tatttgtttg ctcccttcag gtggtttttt
    17401 actgcaaaag ctaaaggaac tcaattttca gaaaagcatg ctcttatgct taaggagcgt
    17461 atgagcagtg tagcagtgct gttttctctg ggattgatag gtcaatggac aattgggctt
    17521 gctgtattta gtattaccag cgcattattg tatgggctta cctttgatca attattatac
    17581 ctgtggtttg gcggtatact atcggttggg atttccattc cttttggatc gtattttatt
    17641 tcaaaaacaa taaaacgatt aaccgatgat ggattgtttg atgaagtcaa gaatgtgatc
    17701 agtgtaaaac ctgtaacaac atttggctca cttattccta tactgacagg ttctacaagt
    17761 atggtcaatg ttttgctggt tatcgtgctg gtaacatttg gcatagtact atcgcatggc
    17821 tacatgaaaa ccatgttcgc acaaaatatc aatagcatat ccaatcaagc ggcgcataag
    17881 ctggatgata tgattgatgg agttatcatt gattgtaaac tggtatcgtt gttacccgat
    17941 agaaacatga taaaccgtat tgtatccttg aaaaaatatt atgctgatta tgccattatt
    18001 gaaaatggta atgttacaac atccggaacg ggacgatatg atgcaattat tgaaaagggg
    18061 atgctatcaa ggaaatcatt gggtcaggat ggtgaattta ttacaaagcc aattgtatat
    18121 gaaaaggata aaaaggcata cgttgttatt ggtgacacaa taaatggaag gatatatgct
    18181 ggtttgattg cggttgatgc tattaaagag caaatcttta aggatgttac cattggaaga
    18241 actggttata tatttgttac ctatgaggat gggagtggtt tggtacatcc acagattgat
    18301 ataaccagcg atgcgttaac tcattatgaa tggagtaaaa aactcataaa taatcataat
    18361 actttaatag catataaatt taataatgac gacaactatg gatattgcac aaaaagtggc
    18421 aggggctttt tattaatagc aaatatcccc aaatctgagc tcaatgaata ttcaaagttg
    18481 ctggcactgc tatcattagg ggttggttta ttctggtgtg ttgttgctac agttatatta
    18541 tacagaacta accgtggcca tttagagcca ttgaagaatg cgcgtgaggt cattagcaag
    18601 gttgcacagg gtgatcttac acaaaatctg gatattataa agggtgatga ggttggcata
    18661 ttatcatctg ctgtaaatat attgattaaa gaattgcgcg aggcgatagc tcatattatc
    18721 aggatatctg atgaattggc ttcatcatca acacagatgt cacagtcaat tacatccttt
    18781 gccgataatg ttcagaatga agcatcaacg gtagaggaga tttctgctac agtagaagag
    18841 atatctgcgg ggatggaaaa cgttgcccgc agagctgatg gacagaatca gagtttatca
    18901 ttgctcataa atttattgaa taatctgtca acaacgatac agacagtagc ggatactatg
    18961 aatgcatcgg taaaagaagt agagcttatt gccgatagat cccgcgaagg gagccaatcg
    19021 ttaaatcaga tgaacagcac gatgcaggtg ttatcaaaaa gctctgcaga tatgaaaaac
    19081 attgtgaaga ttatcaatga tatttcggaa cagattaacc tgctatcgtt aaatgcagct
    19141 attgaagcgg cacgagccgg tgattttggg cgtggttttg ctgtagttgc ggatgaaata
    19201 tcaaagttag cagagcagac agctcagagc attaaggaga tagataattt aattaagcaa
    19261 aacagcactg aaatgacaaa aggaacgcaa aatattacac aggctattca gaatattgga
    19321 ttcatcatca atggggtcaa ggatatatca acacatatag aacatgttgc atctgaaatg
    19381 aaagagcaat tagctatcaa caccaatgta caaaatatga tagccgatgt ccaatccggt
    19441 tctgaagaga taaaaaatgc aactgaagag caacgtgttg ctatacttga aatttcccgc
    19501 tctattacta atataaatga tctatcgcag agcaatgcag gtgctgccga agagatgtcg
    19561 gcaaatgcta ccaatgttga aagcatggca gtacaattaa aggaagtaat ggcatttttt
    19621 aaaatctcgt aattttattn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
    19741 gtgataacat gtcattctga ggcgaagcca aagaatctcg tcgattggag aaagaataga
    19801 ttcttcggtc gctaccgctc cctcagaatg acaatggggc aaaagttccc ttcgtcttgc
    19861 gaaagggagg ggttatgggt aattcaattt tcaatggagt gaaacacaat gagcttatca
    19921 ttagggattg cagggtatca acaaagcggt aaaaagacat tactacaact gttaacaggt
    19981 aattcgtttg acatgaaaaa ggggttaggc gttgcaacca ttcatgacca gcgatttcgt
    20041 acacttgttg atatatataa accccgtaag gttgctcctg caactattaa tatttctctg
    20101 attggtgata ttgacgatga cattataaaa gaagggagta tattcaatac tatagcaaca
    20161 atggatgcat tgtgtgtggt catacgtgct tttaccagtg atagtgtata tcacatcaag
    20221 ggtagtgtag atccattcag agactatgat aagcttatca gcgagtttgt gctgcatgat
    20281 atgatgtttg ctgaaatgcg cataaaccgg ttgcaacagg ataaaaagaa aacagcacag
    20341 caaaaagaac atgaaatagc attgcttaac aaatttttgc agcatctgga aagtggtaca
    20401 ctactgaatg gcttgcaatt atcaaatgat gaaattgcta ttataaaaag ctatcccttc
    20461 attaccttaa aacaattaat tatagttttt aatactaacg ataatgattc aggtataata
    20521 aacaaattca aagcaatgtt tccagaacat gccgtgtatg ccattgccat acctgtaaag
    20581 ctggaaaatg agataaatga attagccagt gaaaaggaac gcatggaatt ttatgcatcg
    20641 ttaaacatag atgcgcctgc cataaacctg ttatgcaatg caatgataca tgctttaggc
    20701 ttacaatcat tttttacagt agggcaggat gaggtaaaac agtggcttat acctgaaggg
    20761 ataactgcac cggttgctgc aggatatatt catagcgata tacaacgggg atttatacgg
    20821 gctgagctta tgcactatga tgattttatt gttgcaggct ctgaagagtt attaaaaaaa
    20881 caggggaaat ttcatttagc tggtaaagac tatattatga atgatggtga tattgtatca
    20941 tttcgcttta atgtataggc ggcgcctgaa tcatgaatga tatattgaag gagcttactc
    21001 atatacggga actatatagt gaaacaggcg cggtggatac cattatcact aatgaatttg
    21061 taacatgcat ttataatttt tttgatagta actatcgaaa ccttccatgg cgcaatacca
    21121 gcaatgcgta ttatatattg gtatcagaga taatgttaca gcaaactcag gtttcccgtg
    21181 tcatagataa atataaagag tttatcaaaa tatttccaac aattcatgcg cttgcagatg
    21241 cagacttata tgatgtgctt tcagcatggc agggattggg ttataaccgc cgtgcaaaat
    21301 atttgtggga gtgcgcaaaa gccattatat tgcagtataa tggattaata ccgcatgata
    21361 taatggaatt gcagaaactg cctggtctgg ggaaagcaac agcggcttca gttgctatat
    21421 ttgcgtataa cataccagcg atatatatag aaactaatgt acggacggtg tatatatatt
    21481 cttttttata tgatacagca aaggtcagtg atgcagcaat tgcgttgttg ctacaacaaa
    21541 caatggatag gctcaatccg cgtaagtttt ataatgcatt gatggattgt ggaactttta
    21601 taaagcagca ttataatgcc tcacgccata gcgaacagta tacaatacaa tcacctttca
    21661 atagttcatt gcgtaaggtt cgcggccaga ttattcgcta ccttgttgat aagaaaaaag
    21721 cgcgtaaaga agaacttgtt atgtatttag attgtgaccg cgataaaata gaaaaagcgt
    21781 tgcattcttt atgtaaggaa aagttagttt gtgaagataa caacatatat accatttctc
    21841 gctcatagta aacaaatatt attgacatgc catcgtagat gttgaataac tacaaaatta
    21901 gattatttta attctttcat actatataaa gagaggttgc ccatgctaaa aagatctata
    21961 ttctttgttt tattcttttt tattatctgt acttctgtta atgtttttgc cgttccggta
    22021 gcttatcgtt cgtggacgta tttagattta acagcattac tttcgcctga atggtcatgg
    22081 acattgttat caagtcatag ctatgagttt gatcgcaata atgatccaaa gggcaatgta
    22141 gaaaaaaaga ctttttttta tgaagttttt accggtccta cctataccat gaagtttggt
    22201 aatttaacgg ttaaattgcc attatggtat tattatcaag gattccctat acgttctgca
    22261 gttaatccat ctgaccacga taaatattac tattcacata atatagaatt tatgccaaca
    22321 ttggaatata aaattgggaa tttcaaattt tggaacagag tcatttttca taataaggtt
    22381 tattcgtcat tatatgattc aagttcaaag aacaaaggat attcgctact aattcgtgaa
    22441 tatttcagag ttgaatacaa tgttaccgat cagtttgccg ttatgatagg cgatgaaatt
    22501 ttccagggga ttattgagga tgatgatacc aaggatataa caccttcgcc actttctcct
    22561 ggttttgaat atggtggttt tacacaaaac agattgtata ttggattcag ctataaatgt
    22621 acaccaacca ttattattac tcctatgtat atgtatcaaa cacagtactt tgatgctgga
    22681 aatgagttaa ctgaaaagga tcattatctg tttacaacat taacgttcat ttttaaaaca
    22741 tactgatgca atacagccca cgggttaaac cgtgggctgt attgctttac ggttggttgt
    22801 acagcgtcta tatcattttt atgcttgcat tttaaatcac aacagtagag tttgttatac
    22861 agtaactatc gcacaaaaaa taaagggaag gaccccatgt catttgattc attacaaaag
    22921 ctttttgaat ttgatgctct gttgcatatg gtgattgaac ccgccagccg ttttgagcct
    22981 gtcaatagcc ggcttgttga ttgtatcgag gtttataaac cacgcgtaat agtgaagatt
    23041 ggcctgggca accctgcata tattgaaagc ataatggagc attcaaaggc attagttgtt
    23101 gtggttgaac cttctgtaac cgtgctacag tcgttttata atgcatatgg gaataaacag
    23161 tggatgaacc gcctgtatac tatagcgggg aatttcagcc agttccctgt tgattattat
    23221 aaagcagata tgattatatg cattgattgt ctggatttac ttgatacggg cagggcaatt
    23281 gatgaattca gacgttcact tcaatttgaa ggggtattct tttatgccgg gtttgtactt
    23341 cctaatgaag atgctgaagg aatctttgat gagatagctc atgttatcaa tccgctacat
    23401 acggattatt accttgaaga tgatttaaaa acatttatga cactcaatga atttggtatt
    23461 gtgaagctaa acacgcagaa gattaatatg aatatattat tgcttgctga ttacctgcaa
    23521 acaagaagca gtgaattaga aaagctcatt gaagaacata aagacacact tactgctctt
    23581 tatgatttgc acagcgatgg aacggtagtg ttaccttaca tgatcgcagc ttttatacga
    23641 cagaaacctt ctcaagaaga agctctatag tataatgggt catatccttc tttgccattg
    23701 tatttttcac tttgttgttt catatagcct aaatatgcca tttcgttaac ttctaatcca
    23761 caggttttga gtgctttcac gacatcgtca aagttgggcg gacacccttt cacttccacc
    23821 gattctttga tggtgggatt attcttgttg gcttttatga tgcaattgcc caacaacact
    23881 gttttatcat aaccaggacg cgcctgcatc tttttgccat ttaaaatttc tacttttgga
    23941 agaggctgcc ctttaaacgc agacatcacc agaatattgc acatattggc aattggtgag
    24001 cagcccgagc atagcgtgtc atcgtatttt ggcaacgcaa ccccttgaat acccatttta
    24061 gcaaatgcgc cagggccggt gttatccgct gtccatgacc aatcccactt taatggctgg
    24121 atgtgatctt ccagtttttc tcctttcact gcataatcgc tcagtaccag cgatttgtta
    24181 tgtcgtgtcg cataggttac aaaatgagct atatcagatg gttcatagcc catgaccttt
    24241 gcaccaataa tatcggcagc taaaatatca gtagaagcaa tgataatatc ttttttgtat
    24301 gcgttgccaa aatggagggc tcctttctcc agtgcatata ttccatcaat aatggtaagt
    24361 tcaggtttta catagtcagc aacaaacggg aaacagtatt ctaaatttag ctgtgggtga
    24421 tgacaaagtt tttttgatga tgccttaaga catcctttca aatttttcag ccccagtgaa
    24481 attttagttt gtccatgagt ttttaataca gggaagttga taaaaaaatc acactccaat
    24541 gcttcttttg ctatcattag atgagtggat gcatcgatgg agcatttttc tgattttgat
    24601 tcattaaaat caacaagctt tactccataa tgtgatgcta aatcagtata tcccaatcct
    24661 gcaaaggctg ccattgttcc aacacctttt ttaacctcaa ccgaaccttc gccaatggta
    24721 atatcgttac atccatattc ttttaagatt attataaggt cttcaaccat ccttgttgta
    24781 gtaaatacac catacggagc aggagggaat aattcatccc atgccaccag gttgggttta
    24841 attaacactt tatcaccggg ttttaacttg ctaaaaccat cacataattc tatagcctgc
    24901 tttaaagaat cagtactttt ggaatatttt accaatgata cactttgcat gatagcctcc
    24961 atttgattaa aagagctact tactataaga aataatcatg ataaatatgc aatacttttt
    25021 taaaaatcgc aaaagtgaat atacaattcc atagcttata aaaatgctta acgattttaa
    25081 gttgatgggc agcaactgca gcaaatgaag caccaaatgc gtatgaccat acaatatcta
    25141 cctgtaccat agtaaaaacc tccttatggt aaaatatgtt gatttgtttt aggtaaagca
    25201 ctttgttttt gtttacaaaa tgcttctatt tgagctaaat attttgagtt tttaccccta
    25261 tctatatatt caggcttttt tagcatttct tcaaatttga tgatggtata gtatggatag
    25321 ctgtcgatta aatggtttac ggcaaaaagt ggaacgccgg taggatctgc atgaataata
    25381 taaaagcatt ttaccttttc cctttgcaat atgtgagcta tcatatatgc tgaaacttca
    25441 tacatcctga caaatccttc aacttgtggg acatattgtt cgctttcagg tggaagtaac
    25501 ccatagcata atacttcaaa tcttccatag tcccaatctt ttgtaacctg tgtccgtacc
    25561 accccatccc ttggctttaa aggccatggc atggccgtgg cgttgtgtat tatcatatct
    25621 tcatcattga atatttttat tgtttctgct atgaagcact tatacataaa ttctttatag
    25681 gcaggtacat cacgcacaag ctgttcaact acctcaggct ttacatacat gattttttct
    25741 gcttttacct gcacaatacc agatggcaac ttccttgtgt acaataaaac atcatctttc
    25801 ttttttaata atttccaatc aggggcatat tcaatccatt tgnnnnnnnn nnnnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnaacatcat ctttcttttt taataatttc caatcagggg
    25981 catattcaat ccatttgctt tttatatcat tactggcaca ttcagtccaa tcctcatgta
    26041 ggcaggttat ttgattttgg ctggatacag ccgaatcata gacgggaata catacaacta
    26101 ttacaaaaag tggtatatac cattttaagc atgtatgcat tgcaaacccc cataatacaa
    26161 aattgctatt ataatacatg ataagaaatc tcttgtcatg taaaaagtag aggttgctaa
    26221 atttgtccaa tcattaaagt atattatttc ctatagttgc ttggttttga ttgtgtaaat
    26281 tttttaaatg ttctgttaaa tgtctttaga ttttcaaatc ccactgccat ggcaatacac
    26341 tatactcatc cgggcaatct attttttgta ctgcactttc atcaatgtca tagttgtcgc
    26401 aacccgataa tgcaatgctg ccggcagtaa gagttacaat aactaaaata acatacagtg
    26461 ttttaaatga tgcggtaatg ccgttcattt tcatggtatg cagcagtaca ggtattgatg
    26521 aaaagcaata tagtattatt ttatataagc tgtcaattgc tattatatct taatactaaa
    26581 aatactggta taacaaaagt aaatgctttc aatagcagtc gtcatctccc catccattgg
    26641 gggggatgat ggaccatcaa aaatttccac ggtagttata ataactatat cttctttatt
    26701 acaaagtaat aatctttatt gacattaatt atcagtactc ttacaatcat tggcatattg
    26761 ctggaggata cacaatgaaa gcacgaataa gcatctccct ttttcttctt ggtttaggcg
    26821 ggcagatagc atgggctatt gaaaatcaat tttttaatac ctttttatat gacagaataa
    26881 tccctgaccc taagtatgtt tccatcatgg tggcgctttc tgcagtcaca gcaacggtta
    26941 ctgccattat catgggggcg ttctctgatt caattggcaa acgcaagcca tttcttctgt
    27001 atggatattt agcatggagt gtctctatat gggtaatacc aatggcggaa tggatcaagc
    27061 cggtaatcgt cgctgcatgg atggtcatca tccttgattc agttatgaca ttttttggtt
    27121 caacatcgta tgatgcagta tacaatgcct atttaaccga tgtaactacc gatgccaacc
    27181 gcgggaaagc tcaagggtta ctatcgcttg caacatggat tgcgctttta cttgtttatg
    27241 gcgcatcagg gcaaattgta gaatcaatgg gatacttttt cttctttggt gcagccggtg
    27301 cgctggtatt actatcaggt gttataggcg gcattgcagc agtggagcca caaagggcga
    27361 tagttaccga tagtaagtct atcgtaaaaa gaataagggg aagcttaaat atgcaagcac
    27421 tgcggcacaa taaaaatctt ttgtatgtgt tgcttgctat aggattctgg gggatggcat
    27481 tcaatgtttt tttcccatac ctattgattt acctgaaaca ttttttgaaa attgatattg
    27541 ctacgtcatc ggttattata gcagtatcta tctttattgg cggaatagca gcttccatcc
    27601 ctgccggcat tattgctgat aaaatagggc gtaaacctgt tgctattttt gctgtgatat
    27661 taatttcatt ttcgcttatt gcattttcgt atgctcgttt accgatagct atcggcatat
    27721 tagcaacctt atggataatt gctcagacaa tatggacagt tgctacagga gcatggagta
    27781 aagatttatt ccctgaagag aggcgaggtg agtttgcagg atattttaca ttgttctatg
    27841 ttgcatttac aatgatacct ggtccgctca ttggggcgat agttgctgac agatggggta
    27901 tacgggcaac cattgatggc aaaccaggta ttattccaac acctgagata tttgttgtat
    27961 ctgctgtgct cattttaatt actattatcc cgctgttatt tgcacaagaa caatagtagt
    28021 aattgtgcta ttgggtattt ttgatatatt tttcaatagc atcaatgtca tcttgtgaca
    28081 gagcgcacgg tgctttgtaa tctgcattga tttgttttgc tgctttgcca acaaccttcc
    28141 agaatattcc aaaattatca atatttgctc tggcaacaaa cataggtcct ctgaatcctt
    28201 tcatgatgcc cggcatctgc atatatcccc acacccccga agcaaattta taccattgaa
    28261 cagtggttgg tgcaaaagcc cactctacag ttttatactc ttcaactaac gcaccaccgt
    28321 atgtgcatat tgccctgcca tccttatttt caatggtaag ttgaactttt ggcattgagt
    28381 cacaatctct tcgtgacatg gcgaaaacct gtttgttcct cacctgaccg gtgtatttat
    28441 tttttgtcaa aatccagtca ttgccaccaa ctgcttttgt gcccaatttt tgggtcagtt
    28501 cacttctgtt ccattcatca cagacaaatt ttgtaaattc aggacttaaa aaaacaatag
    28561 aattgctctg agcatgggtc aatgaaacat tgaagtaaag agcaattgta aaaagggtaa
    28621 caactgataa aatgaaaatg aattttttca tgggtatcac ctcgtattaa aaattgtatt
    28681 ggtatgaatt ataagatatt tagttattaa aatgtcaata aaattattaa ataataataa
    28741 actatatatc taaaatatta aaaagaaata attgtatgta cgatagttac tatctattat
    28801 aatacagtat taatttttat atttgatttg acatgattgg ttgcatacga ttacatattc
    28861 aaaatgaggc agacagcacg acatggtgca atgcatccag aaggcaacac aatgtaattt
    28921 ttttaaaaag tcatcctgaa caaagtgaag aatctcgctg atttgtgaac gaatagattc
    28981 ttcgggaatg cgtcccttag aatgacaatg tcgagaacgt catcctgaac aatgtcctta
    29041 actggcttca ggatctaacg ttacacatct ggattccggt tcacgcccgg aatgccattg
    29101 aagggataat catattttag ggaggctgtc atgaaacggt ttatcatgta cagtattgct
    29161 ggtattattc ttgtgattgc agtaagtata tcatatctga taataacaga gtatatatta
    29221 attcgttttc ctgtgctggt acaaacaaat cctaaaatca catttataat aggagattca
    29281 tattttcgtg aagatgaaaa ggcgcagtgg aagatagcgg ttgttggtca aacattgtat
    29341 caaggttact gggtaaagac tgaccccgat tcaagaatgg atattcgttt ccatgataac
    29401 accgcaatac agctatcaga aagttcagag atggctatcg attcattaac agtaaaaaaa
    29461 ttaggattac agatacatac aggatcgatg tatgggaagt ttgataagtt gtttaaagaa
    29521 catgatctcc atgtaaaaac tccaacggca tttgctgcaa tacggggaac tgatttaggt
    29581 tttatggttg ttccagaatc ttatggaaat gatagtctta tgggacaaca aaaaaaatct
    29641 gcatataaca agggcaagaa taaaggacaa acattaaaaa caaaaggtgc gactcaaaat
    29701 cctgattcag gtgaagcaga cttgcaaacg ctggtttatt ctctttcggg gattacggag
    29761 atatacaatc caatatttga tgatcagaag gttttgctgt catatcgcaa taaagtcatc
    29821 ataggtgaag agagcacacc aacaaatcca gaagagttga gtgaggatga aatcaaaacg
    29881 ttacaggaaa aactaaactc aatccatttt gatgaagtat tgttaattac tgataaaata
    29941 acatttaatt ttggtagtgc agaaatactg ccttcatcgt atcctgaatt ggataaaata
    30001 gtggaaatct taaaacagaa aaaggttcgc gtaaggattg aaggacatac cgatgaccag
    30061 ggtgatgcag catttaatca gcaactttca gaaaaaagag ctgaggcaat aaaaaattat
    30121 cttgttacga aagggcttga tcctgagcgt tttgaaacag tggggtttgg tgaatcaaaa
    30181 ccaatagctg acaataaaaa tgaggttggc aggtcacaaa atagacgagt tgagttcctt
    30241 atcatagaaa aataaaaaaa ccgtgctcaa tttttatttg acaacaatac accttccaat
    30301 agtgttgcta tcggaaggtt gtccgagtgg tctatggagg cggtcttgaa aaccgttggg
    30361 tgaaagcccc gggggttcga atccctcacc ttccgaataa cggagagatg cccgagaggc
    30421 cgaaggggac ggtttgctaa accgttgtac tggaaacggt accgagggtt cgaatccctc
    30481 tctctccgaa tggggaaatt acactattac agcttacaga tagccaagta cagtaaaaaa
    30541 ctctacgcac aatttatgca ctattatgct gtggttaagc aattttgctt tgaacacaaa
    30601 cagcagatta ttgcatctat tgcgtatata ccatatatcg gatggttgta tcccttatat
    30661 gcctgtgaag gagatggagt aatccagaaa catgcaaagc gcggattatt atacgcaggt
    30721 gtatttgttt ttgctgctct ggttttattt ttgttgtcat ttatactgcc acgatcatgg
    30781 cgcctgctca gttttatagt taccattgtt ttgtatatca attattttat ttactatgcg
    30841 atagttatta ttcatatatt caatatctgg aaagggaaag atttttctat accaaagttt
    30901 gaaatttatc ttgaaaaaat tgagcagtat atataaatat tattgtattg tttttataca
    30961 tgcgtccata gctcagttgg atagagcgtc ggactacgaa tccgaaggcc agaggttcaa
    31021 atcctcttgg acgcacataa gcgatgtttc attacacctt aatttttatg ccaaatatat
    31081 acgttgttaa atcttcagta tcaactgctt tttcagtcaa ttgtaataac ccgctcaatg
    31141 taagtcacca tagttaattt ttaaaatttc aggtgggggc ccccaataaa attggcagca
    31201 gtgtatacct ttaaccttaa aaaaatcaaa agtatacgaa cgattttcgt aatatgatag
    31261 atagttatcg gttaatgatt cttttctgcg atgaattacg tagttaaaaa attcatccag
    31321 cacagtatcc acacaaaatt caatagcttt ggcaatggaa accttccaga tttttttaat
    31381 atcaagtaaa aattcatatt cactccagct gatacataga tgaaaacgaa tccatttgat
    31441 atctgtattt ctatcacgat aggagatgtt tttaaatacc tgtgttttcc ttttttcttt
    31501 ttttgcagca tactgaatga ggtaactaat aagtttgctg acggatacat ctaatatttt
    31561 tgcataatgt ttgagtacta taatatggtt acttgatatg caggtagttg tttcaaaatt
    31621 cataaaaccc ctccaaagtt gtatgtagaa atttgctgat acttttaaga ttatttttat
    31681 gtgattaatg attaaaaact tttactgcta ttttgctatt acaaatgcat tgtaaaattt
    31741 tctacacagt ctcttgatat aattcaaaag actgtgtaat taaaatttat gtaaagtatt
    31801 aataaattaa ctttttatga tacggcatat tctgcaacct tgtcaccaac ctgagttgta
    31861 gttaagccca ttttgccggc agcaagtgac ttcatatccc gttgcaatac atcaataaca
    31921 cctttttcaa tacgtgcagc agtcttttct tcgcctaaat gttccatcat catctgggcg
    31981 gcgcatattg ctgccagagg gttgataaca ttttttccag tatattttgg agcagatcca
    32041 ccaattggct caaacatgga aacaccttca ggattgatat tacctcccgc agcaatcccc
    32101 atgcctccct gtgtcatggc gcctaaatcg gttataatat cgccaaacat attcccggta
    32161 acaagaacat caaaccattc aggatttttc accatccaca tgcaggtggc atcaacatgg
    32221 tagtaatcgc gtttaatatc tggatattct ttctgtccaa tttcatgaaa tgctctttcc
    32281 cacagatcat acacataggt taatacattt gtcttgccaa caagtgctaa tgtttttctt
    32341 ttattacgtt tgcgggcata ttcaaatgca taccgcaaac atcgttcaac ctggaagcga
    32401 ttgtaaatca taatctgcat tgcttcttca tgcggcgttc ccttcatggt aactccgcct
    32461 ataccagtat acacgtcgcc tgagttttcg cgtaccacaa tatagtcaat atgttcagga
    32521 cctttatctt tcaatggtgt ttcaacattt ggataaagct tgattggacg tagatttatg
    32581 tactggtcta attcaaaacg aagtgccagt aaaataccct tttctaatat acctggtttt
    32641 acatcgggat gacctattgc tcctaaaaaa atggcatcat gtttttttaa ttcgtcgata
    32701 gctccttcag gtaacgtctc acctgtttta agataacgct cgccgccaaa atcataattg
    32761 gtaaaatgga gagatatatt ctcaactcca gcaacagctt ttaatacttt gacaccttcc
    32821 gcaacaacct ccggcccggt cccatcacct ggtatcacag caatgttgta cgattttttc
    32881 atatttatct cctaaaataa aattatgagc cactgttatt tatggcttga ctcgtaatct
    32941 aaacaattat cagaaatcct gaatcagtat tcagaatgac tatcaccaaa tatttttacc
    33001 ccattttgat gtctatattg ttgccatcaa ttctttaaca gtcataatga tccaataggg
    33061 acttttcata agattatata tttctataat aatagcactc cgcttagaat tataaaattt
    33121 tttgatgctt tgactgctgt aataatacta tttatgatag tgtagtactc aatatgatta
    33181 ctactgtgtg attatactac agtggggcat acccatatta atcagaaatt tttgttatac
    33241 atttattagt actgcgcaag ttcaagcttt aacaatacga tgcaaattat gcgctgcttg
    33301 aaacattgca tttactgtgt tgagtaagct tgatttgcag ctaaagacca atacagtgag
    33361 ctatcgccag atgtaagccg tatagtgttg tcaggtcata aagaaaatga gcaaccatta
    33421 ttggcagcaa agagtttgtt tgtatataca ggtatgccca tagtgctccc cacaatgctg
    33481 cttcaagcca tgtgccggga cccattgacc agtgaatggc tacaaaaaga agcgttgatg
    33541 caatgatgag tatccattta tttgatgtta taagacgaag ctgatgtatt gcaatgccac
    33601 ggtagattat ctcttcatag atgcctgcgg tgattgaaag gtacagcgca tacaaaaaaa
    33661 catacaaagg tttccaccct ggcaatggga aataccagaa cgatgcaagg gtaaatccat
    33721 agtgtttttt acaatattcc agtgcgatag ttagtatggc atacgctaca aatatagcaa
    33781 taaaaagtac tacaccccat agccattgtt tgggctgttt cacattgatg cccaatcttt
    33841 cccatgtaaa ccacccggca tcatatgcaa catataaaag cgaggtttgc cagccaatgt
    33901 agatgatgat gtcaaggata agattaagct ttgttgcatc tttcggaata agaggcaggt
    33961 agcaatcatt cagcaatagt ggtgttgcta aaattagaag tcgcagtgta acggttatta
    34021 tattgtgcat aaaaaaatct tatccccttg gtttaacctt ccaggtaaat gctaacagca
    34081 caatagagat aacagtgcat gcaacgatgg agtagagcac aatgttccat cgggtaacaa
    34141 tatcgtatgt tgtggcaaag taatccagaa taaagccaaa gccttttgcc tgaactgtcc
    34201 tgccaagata tccaaacatg ccaataaaac ctgctgctgt acctacagct tttttgttgg
    34261 tcaggtcaag ccccataaca cctaacatca taaccggcgg atacacaaaa aatccaatga
    34321 ccgaaagcat tgcgtagtaa aaccacaggg atggttgcgg gtataccact attgcaaaaa
    34381 atgccagcac taccggtatc atacatagca tacttaccat gccgcgccgt ccctgcaatt
    34441 tgtccgaaat ccagccaaac aaaattgttg atgggatgcc tgcaaactca agcaccataa
    34501 tgctgaatcc accctgtgta atgtttgctc cctttatctc acgtaaaaat gtcggacccc
    34561 agtcaagcat gctgtagcgg gcaatataga caaagaagtt tgcaatggca actacccaga
    34621 gcaatttgtt tttgagtata taatctacaa aaagagtttt tgtcgatagt tccttttcaa
    34681 tgtgttcatg tgtttgagga ggataatcgt ttttgtatac ctctacaggt ggcagaccaa
    34741 ccgattgcgg tgtatcacgc agttgccaga aaagatagag ggcacacagt atggcgatag
    34801 cacccgggat aaaaaaagca ttgtgcagac caaagtgttt tgcacaatag gcagcaagca
    34861 taccggcaac ccctccgcca acattatgtg caatattcca tactgagaag atggtgccgc
    34921 gctcactgac gctaaaccag tgaccaatgg acctgccgca cgggggccag cctgcaccct
    34981 gaataaatcc attcaaagcc catagccaga agtgaatggc ataactctgc gttgcaccaa
    35041 atgcaaaatt acataaagcg gttagtataa gcccaaccgg catgaatacg cgaacattgc
    35101 tgcggtccga gacagagcca agcaaaaatt tgccaatccc atacagtaac gcacttattg
    35161 ccagtatgct cccaatctgt gaatggtcat agccaaacgc tgtttgcata tctctggcaa
    35221 cagtggaaag attatttctg actatataga aggttgcata cccgatgaat gttgcttgca
    35281 ggatgtgcca tcgcaatcgc gggtaaagtt ttttcacctt ttcttctggc agttgtggtt
    35341 ttgccggtgg cggtgcaaag agtgatagca ggttcataat gacatccctt aaagtaaaaa
    35401 gtgtgatgca gtaaatgagt acatgatata tgcgtatttg caaatctttt ttattatgaa
    35461 aacctgcaaa attgttattc cgggtttgac cctgaatcaa aataatttta aatgaagcta
    35521 tattgataat agcaaagtag aagatatttt ttattatttt tatgaaaaga tatgtctttt
    35581 atacagtctg taaattcagg atgactatcg ccagatattt tcatattatt attgtggttt
    35641 aaccttccat gtaaatgcta acagcacaat tgatataaca gtgcatgcaa cgatggtgta
    35701 gagcacaatg ttccatcggg taacgatatc gtatgttgtg gcaaagtaat ccagaataaa
    35761 gccaaagcct tttgcctgaa ctgtcctgcc aagatatcca aacatgccaa taaaacctgc
    35821 tgctgtacct acagcttttt tgttggtcag gtcaagcccc ataacaccta acatcataac
    35881 cggcggatac acaaaaaatc caatgaccga aagcattgcg tagtaaaacc acagggatgg
    35941 ttgcgggtat accactattg caaaaaatgc cagcactacc ggtatcatac atagcatact
    36001 taccatgccg cgccgtccct gcaatttgtc cgaaatccag ccaaacaaaa ttgttgatgg
    36061 gatgcctgca aactcaagca ccataatgct gaatccaccc tgtgtaatgt ttgctccctt
    36121 tatctcacgt aaaaatgtcg gaccccagtc aagcatgctg tagcgggcaa tatagacaaa
    36181 gaagtttgca atggcaacta cccagagcaa tttgtttttg agtatataat ctacaaaaag
    36241 agtttttgtc gatagttcct tttcaatgtg ttcatgtgtt tgaggaggat aatcgttttt
    36301 gtatacctct acaggtggca gaccaaccga ttgcggtgta tcacgcagtt gccagaaaag
    36361 atagagggca cacagtatgg cgatagcacc cgggataaaa aaagcattgt gcagaccaaa
    36421 gtgttttgca caataggcag caagcatacc ggcaacccct ccgccaacat tatgtgcaat
    36481 attccatact gagaagatgg tgccgcgctc actgacgcta aaccagtgac caatggacct
    36541 gccgcacggg ggccagcctg caccctgaat aaatccattc aaagcccata gccagaagtg
    36601 aatggcataa ctctgcgttg caccaaatgc aaaattacat aaagcggtta gtataagccc
    36661 aaccggcatg aatacgcgaa cattgctgcg gtccgagaca gagccaagca aaaatttgcc
    36721 aatcccatac agtaacgcac ttattgccag tatgctccca atctgtgaat ggtcatagcc
    36781 aaacgctgtt tgcatatctc tggcaacagt ggaaagatta tttctgacta tatagaaggt
    36841 tgcatacccg atgaatgttg cttgcaggat gtgccatcgc aatcgcgggt aaagtttttt
    36901 caccttttct tctggcagtt gtggttttgc cggtggcggt gcaaagagtg atagcaggtt
    36961 cataatgaca tcccttaagg taaaaagtat gatacagtaa atgagtacat gatatatgtg
    37021 tatttgcaaa tcttttttat tatgggaacc tgcaaaattg tcattccggg tttgacccgg
    37081 aacccagatg ttcaacgtta gattctgaat cagcgttcag gatgagtttc tacgtttcaa
    37141 aatgacacag ttcacattgt cattctgtga gatgcagtac tttgtgtcat tctgagggag
    37201 cggttgcaaa tgaagaatct attctttaat tgagatgctg gattacgatt aaggatgacc
    37261 tcttgattgc aatttcagat acttgatata gtaaatgtgg tatgttataa taaaaatgta
    37321 tttgaaattt cttagcctgt atttattagt attgatatca atgtatctca atttgtatta
    37381 ttgagggagt gatgaagatt acaaaaaaac gcagtgatgg attaaaacga caatcagaaa
    37441 taatgatgat tgcccttgaa ctattttcaa aaaaagggta tcacaataca aaattggaag
    37501 atattctgaa aaaagctgat atagctaaag ggacatttta tcttcacttc aatggtaaag
    37561 atgacttatt acatatgata gtagatagtc aactggaaga gatgtaccgt attttaagtg
    37621 tacttgatat ttcaatggat aaaccattaa ccgaagtaat taatatctat agaactatcg
    37681 cccaatacct tattcgcgat ccaaaaatta aaacgtttgc acgtattatg ttaaaagaag
    37741 caatgggatt ggataaaaca cttttacaaa aacttaatga tttttataat aaaattattg
    37801 ccatgtctgc agattatatt gcaaaggctc agggaaaggg gagggtgttt cccatgcttg
    37861 atccttattt cacgtccatg tgtattgtag ggtccatcaa agaattggtt tttcagtggt
    37921 cggttaatga tgaatcatta gatttagaaa aagctattat gacagccgcc gatatctatt
    37981 ttagagggat ggtcaaagga tgatatttat tacttgacta aaaagtgata tgtataattg
    38041 tatgaaaaaa tgtaaaatgg gtgtattgta ctataacttc tgaataagga aaacgtagaa
    38101 ataatatgaa attgtccgag attaaaaaaa ttcttgatgc tgatgtcata gtgggagaag
    38161 aaatgctcaa tactgtagat gtagaaacag cttttgcatc agatttgtta agtgatgtgc
    38221 tggcatatgc aaaagagcgt gcattgttta ttaccggcct taccaatccg caggtggtgc
    38281 gtacggtgga agtccttgat cttattggtg tggtatttgt acggggtaaa gttccacaga
    38341 tggatacggt agagctggca aaacgtaaaa atataccatt gctgagaaca aaatgtataa
    38401 tgtttgaaac ctgcggaaga ctttatggtg caggtatgaa agcttctatt gaaaaagttg
    38461 attaaaaagt ttagcgcatg cagtgtgcaa atcatatatt ttcatataca ttgaaaggtg
    38521 gtgattttga taatgccggg aggatttcga cttcgctcaa gcgtgagttg agaacgcggg
    38581 aatatccttc ccatatgatt caaagggtag caatagtatt atatgaagcg gagataaatg
    38641 ttgtttcgta taccaccgtt ggatattttt ttgcatattg cagaccaaat tcagtacatc
    38701 tggtggtaaa ggacaaagga aaagggatag aaaatatcag gctggcagta caggagggtt
    38761 tttcaacagc aaccgatgaa atacgcaggc ttggatttgg tgcaggtatg ggtttgtcga
    38821 atattaaacg caatgctaat gttatgcgca tttgctccag accaggtgaa ggtacaaaga
    38881 tatgcataga tataacatca agcaaccatt atggagattt gattatggac atcactgtaa
    38941 acgaaatcca gcaaaaaaca aattttgaac tgttaaccac aaaagctgat tgcacaaaag
    39001 gcattactgg tggttatgtc ggcgatatgc tttcggatgt taacgctaac ggtaaaaaag
    39061 gaaatatctg gattaccatt ttaacgcatc ccaatgctgt ggcagttgca tcattaaaag
    39121 attttagcgc tattgtagtg gcaggtggaa taaaaccccg tgatgaattt gtcaaacgtg
    39181 ctgatgagga ggacattcct gttatgtata ccgatttatc agcttacgag gttgtagtgc
    39241 ttttatcatc gctgggtata acagcaaatc attaaaaaat gtttggcgat agttaccggt
    39301 gaagctatct ccctattaaa aaattaattg catagccagc agcggcaagt cccattatac
    39361 ccggcatata cgataaactt ccccttgtat tgtttgtgct aacattggtt gtactgcctg
    39421 tacgttcctc gtggaaataa acgcacataa atcctccagc aatcccaata tttttgagac
    39481 gctttcgcat ggtgcgtgcc agcggacagt actgtgtttt actaatgtcg ctaactcgta
    39541 aactttccgg attacatgca ttagccgcac ccatacatga aatgaaagga atatgcctcc
    39601 ccagcaatgt tgtaataagt gatgtttttg catcaacgtc atcaatggca tcaacagcaa
    39661 aatcaatgtc gtgagggatt aatccgtgaa cattatcagg tgttatacgg attttatttt
    39721 tattaatcat accatcaggg ttgatatctt ttgctctttt ttcggcaacg tcaacctttg
    39781 gaattcctat tgtactgcct aaagctaaaa gctgccggtt gcaattggat atctctacag
    39841 tatcaaaatc aataatatga acagtaccca ccccagcgcg aaccagtgct tcaaaggcat
    39901 atgaaccaac gccaccaagt ccggcaataa aaactcgtgc tgtgaatagc ttttcaaggt
    39961 tttctttgcc tattaaaagt gaagtacggc taaatcgctc ataataattt tgattaatat
    40021 tcatagttaa aaaacctgca ttgtaatata atttttttaa ttttagtgca gtatttattc
    40081 cttaaaaata atgttatata aaagcaaggt acttgcccgc tgtattctgc agcgatgata
    40141 tatattcatt agtgttttta taaaaataaa attatagcgg tttgtattgc tattttaaag
    40201 caattttttt tgttttttat taaattctat aacttctggt ggtggtcccc agtagaactt
    40261 caggcaatgt atgccttttt ctttatgaaa atcaaatgta tacgacctgt tttcataata
    40321 actggttaga ttataaggat agttatcgga attaatctct tttaatcgcc tgctgaaata
    40381 ttcaaaaaat tcaaccagca cattttctac gcaaaacgca ataatgttgg caagcgacat
    40441 cttccatagc ttcttcacat ccaggcaaaa ttcatattcc gactgatata acaccagatg
    40501 tactcttttc catggattgt ccttatcccg ttttctatag cagattcttt taaatgcaat
    40561 agctttcttt ttttcctttt ttgcagcata cagtatcata tatacaatac atgtcctcag
    40621 cgacacatct aactttttac agtaactatc tagaatttct aagtgtttac acgatataca
    40681 ggtagtagtt tcaaaatcca ttgcttcctc ccagagttta tattttttag atgctaaata
    40741 ttatttggta tgataaaaaa attatatgtt caaattcaac aatttttttg cgttttgtgt
    40801 tgtagcttct gctatctctt cttcattgtt accaataagc tcactcatag cttttaccac
    40861 atagataata ttttccggca ggtttatctg tccacttttt tccacagggg gtatatcagg
    40921 gctgtcggtt tccagtaata catggtcagg atatatatgg tgtaaaacct gcaatttttt
    40981 tttgctgttg cggtaggtta gtgctcctcc aaacgaaaag taaaaaccat aacggataag
    41041 ctgccgggct atatcagcat tccccgaata tgaatggata acgccgcctg cgtttatttt
    41101 cccaatacgc ttcagtgaag caataagctc attgaaagct cctctgcaat gaacaatgac
    41161 gggcttgttt aaatcattgg caatagcaag ctgttcttca aaaaattttt tttgcacagc
    41221 aaggtccatg tcggtttttg catcaagccc aatctcgcca atggcaatgt atttgctgtc
    41281 ggttatcgca tacagctttt gcaattcttc aaagtattgg tttttgatat accacgggtg
    41341 tacaccagcg gcaaagtaaa cctcatgaaa gctatgacta attgtttgcg atagctccca
    41401 ctcatcagga actatcgcac aggtaataaa agcgataaca ccaacctttc tggctgcatc
    41461 aatgattgca tcaagccggc cgctgaaatg ctgactttcc agatgacaat gtacgtcaac
    41521 aagtcgcatg tcattcgcca atcactttta tcagcacacg tttggggcgc tgcccgtcaa
    41581 actcagcata aaaaatctgc tcccatgggc caaaatccaa tttgccgttg gtcactgcta
    41641 caacaacctc tcttcccatg atgcttcgtt ttatgtgagc atcgccgttg tcctcgccag
    41701 tacggttgtg gcgatatttg ctgattggtt catgcggtgc cagtgtttct aaaaaatcat
    41761 caatgtcatg gataagccct ttttcatcat cgttaatgtg taccgaggct gtgatgtgca
    41821 tggcattgac aaggcacaac ccctctttga tgccgcttct tgtaaccaca tcctcaacca
    41881 tggtggtgat gtttatgtaa tcacgttttg ttctggtatt gaaggtaagg tattcggtca
    41941 ttgatttcat gggttgcctc cttttttttg tccgatagtt atcggtaatg atgataatgg
    42001 tataaaagtt tacctgttgt gtcaatacat tctacgctca atcaccatga atacatattg
    42061 actttttgat tgcagagcag tataataatg gtaatggtag tggggaaaaa taggttgaca
    42121 aatactatac gcatagtaat attaatatac atactagtat gtagaaaatt ataactttag
    42181 cgggaggctc ttatgaaacg ctttgttata ttgtttacca tcctgatagc tgtcttcata
    42241 gcggcagcaa tttattcaac caacagggtt gaaacagaac ctgcatattc tgcttcgcct
    42301 gttgactgcg ggtgggatgc agttaagcca cactttgtga atgatagccc ctgcccagga
    42361 gcggtgtact gttcgtatgg aaattgtggt gaatactgct gtccgtgggg atatttctat
    42421 tccaatccat gtacatgccg atgctatcgt acaagctatg atgcagggca ggattgcaat
    42481 agctatttca gatgtaatta gtatatctgt cttatatgaa tttactatta tattaccatg
    42541 tcaatacatt tattatcctg aataagtttt tgacgacctg tatgcttttg ataaagattt
    42601 tatttttaaa agcaaaatta aaaggcaaaa tttttattga caagttacat agtataaatc
    42661 taatagcctc gtctttaaaa tgaaagtttt tacataattt tttttgaaaa tattgtttca
    42721 gttagtacat taatttttac tatcatcctg tagtttaaat ggtaaaatca cagctttgtt
    42781 gtgattaatt accatggtgc aggttatacc attcccctgt agctcagtcg gtagagcgag
    42841 tggctgttaa ccaccaggtc gcaggttcga gtcctgccgg gggagcatac ataaaaccat
    42901 aatgagcatg actttttttt atcaatccca ttcataatct cgattaacac attttttaat
    42961 gcaataagta ttgtataaag gctctacttc tactaaacgt ttacaagaaa aatgtatatt
    43021 agtttgtact gagatgtatt tataaggtag aaaagcaacg aaaaagagaa aacaaattat
    43081 acaatagagg tattggagta tagtaacatt tatttactga agctaaccac caaaacagca
    43141 gatttcatct gcaatccgtt taaaaatgtt tcgttgtacc attgtcacag tgtttatatt
    43201 ttttgaaaat tatggatatg gtacaataat acataaacaa attatatctt gacaaattgt
    43261 agtttctaag taacaattct ggcatgaaaa atgcaaaatc ctttcaggac attaaatata
    43321 ctgaaaaaga aaacaaaaaa aagataatca tagacgctgc cgagcgtgta tttgccacca
    43381 aaccttttaa tcaggtaaca atgcgtgata ttgcaaaaga agcaggcatc tcggcaggtg
    43441 ctatctaccg ttatttccct gatcaacagg cgctgtttgt tgaagctttt ttactgggag
    43501 cagaagatat aattaatgaa atggaaataa ttattgcaac aagtaaaaat cctcttaaag
    43561 ataccatcta tacattttta agttatttaa ataagcacga tccttatttt cgcatgatga
    43621 ctcacttcat gctggatgca aacttgagtc ccgatttaat aaataagctc aatgaagtcg
    43681 aacgccgggt gctggatcaa tttacaaaac tttttgcttc acgtactaac gcacgactat
    43741 tagcgcatag cttatttgca tcattaaatg gagttatgat tacatttcat aattaccccg
    43801 gccgaagtaa aaaagaggta cgctaccaca tgaatagatt aggcgaaaca ttataccaat
    43861 tattttcctg ctaacgcagc atcttccctt gcagcttcaa cctcatgcaa tgcacaattc
    43921 tgaaattcat gcaatagctt atgagctgcc tcttcccaca gcagacatag ttcagcattg
    43981 tcctgcccag gataaatgaa attacttgat aaacatctgt caaatgctgc agtcacctcg
    44041 tcaatgacac aactatctgc attgtacagc aaatcattga taaactgatt aagcggatac
    44101 catgatgcag cgcgttgcat atcacgatag atttgtgtaa gcagtacatt gcgcggtccc
    44161 tcccatagtt cgtttatcat catatcgcgg aaaagacggg gtaaacttga aaaatcttcc
    44221 ataacgccat gaccgccaaa caccgagatg gctttacgca atacatctgt agcatcccat
    44281 gaggcaataa tcttttgtaa cattataagg atacgtaccc tgaaccgttt ctgctgggtt
    44341 gcgatgtctt cgtcagtggt aagcccccct ttcaaccctc ccggcaatga ttggaacatc
    44401 tcataaatct taaaagcgcc tgctaatgtt cgtttcgcat aaaggttaat ttcattgagc
    44461 tgtcgcttaa gcaatggaaa ctggtttatg ggaaatccaa atgctgttct aaagtttgca
    44521 tacgcagtag cttcgcgggc agcgcgtatc atagctgcag cagcagaaag tcccacggtc
    44581 aaccgtgaat gcgttagtac aataccaaca acattggcaa caccacgctg tacagctccg
    44641 gttttgtagg cgatagttcc attgaaggta atctctgcag taggcagctc ggcagtccct
    44701 aatttccatt ttagcctgtc aatggtgtag ttattacgct tttcgttttg tttatcaagc
    44761 cacgatggca taacaaacaa cccaacatgc tctgacccct ttgggcgtgc agtgatgaca
    44821 acataatcag catgcgttgc agaacaaaaa aactttttgc cataaagcct ccagtggtca
    44881 ccttcttcaa cagcctctaa atcatttgca gcaacatccg atcccccatg aatctcggac
    44941 aggtattgtg cgccaattgc aaaattgcca tccatgcctt ctttgcagtg ttgtaaaatt
    45001 ttttccagtt caggatgatc atggtatctt tcaagtaaag ccaccagacc ttcagtgcat
    45061 acaagcgggc aggcaatacc ggcctcccca ttttcataaa tcaatatcat ttttattaag
    45121 cgttcccatg gggatgtttt gcttgaaaac aaaccctgtg aaaacacctg cttttccatc
    45181 tcttctaaag ctttagggcg tactatcctg tcaatacggt ggttgtgtcc atcatagtgt
    45241 tgcataaata ttctgttatg tggccatgct atgtcatcag atatatcacg ccagctaaac
    45301 gatgctaatt ttgacaacct gcgagcttct gcatcaagct gtggaaaatc atttcccgca
    45361 aaatgcatgg ctaccttttg aagaaagggg tcgtcggcat aaaagttaac attgtttcgc
    45421 cagtttaaaa attcatcaaa gctgtaggtg ttgttcagtg ccataaattc cctccgtatg
    45481 taaatacaat aaacagtgtt tattgttgtg aacaacgttc atttgtcaag cacaaaaagt
    45541 atgtaaaaat cgctgactaa catgaacaat tgcataggcc ggtaaatata ggtaacaggt
    45601 cattatcgga tgattattta aaaaatgtat attctgtatt attgatagtt gtgtggaata
    45661 cgcataaaaa aaagtgaaaa gcttgactat ggctaatctg gggttaagta tatgctgaaa
    45721 attatttcat atgtgcatgg aatttaaaat ttgttgttat ggttatgcta tcatgactgc
    45781 atccagtatt gaagagatac ttgctatcat tgcagagcgt tttgcaaagc cttttgttgt
    45841 ggacgatgct accatccatt atgctgaatc aacattgggt gttgaccaac atgcaatcat
    45901 ggattacctc aatgatgatg agtatggcat gatggatttg attgtttatc cggaactatc
    45961 gcttagaaaa aaaattgagg gctacatacc ccggcatggt tttaccagcc atcaggtgga
    46021 acagcttatt gaaaggctgg ccagcgccat accatgcgtt gccattaatg tgcataacac
    46081 aacggttgat tgttacagtg caatgtgcat gcgctcgtat gtgcataaac tttttttgac
    46141 gaagagcaat atcccgctgg atgctatacc tgccatgtat tataacaggt atattgctgc
    46201 gcgtgtagcc atacgattat ataacactga cagcaacctt gatgttttac acaggcttgc
    46261 ggttgggtta gccagcgact ataatgaaga ttatatattt gatagtattc aagtattgat
    46321 tagtgttgcc gggaatgccg gtaacatttt tgattgtatg gcactgtata agtacacagt
    46381 acaaaaacag atactggcta tgcatgaatt tcatgaactt atgaaatcat acagcatgga
    46441 atatctcatg tccatgagat ataaccctcc catgatagac gcagcaaagc ttgagtatca
    46501 gatacgctta atagacagta tatgcatggt gctgtttgga aagccagcgc cagggtatgt
    46561 gccggatttt aatgatacat ggaacgattg aaaaatacac aatgcttacg ataattaagg
    46621 gattgataag ctttgctttg tattacaaca taaatgctaa taaatgcagt ataaaaatag
    46681 ttactggagg gacattatat gtcacagtag ttatgtatat taacaatgaa tatggatttt
    46741 ttattaaaat aagttgcttt atgcaagcac tgcgtgctac aactaatgaa tgctaaaacc
    46801 ccggagaagg ggtattagcg gataatgatt attattcaaa cccggtcagg ggtgatgaaa
    46861 tgatattgtg catcatttta tttttggagg aataacaatg agtaacatgc aacagtacga
    46921 taattttatc aatcattatg caatacagaa aacactacgg tttgaactgc aaccaatagg
    46981 aaaaacacgg gagcatattc aaaaaaacgg gattattgag catgatgaag cgcttgagca
    47041 aaagtatcag attgttaaaa agattattga tcgttttcac aggaaacaca ttgatgaagc
    47101 actttcttta gcagattttt caaaggatac tgctatgtta aagcgatttg aggaattata
    47161 ttggaaaaag aacaaaaatg aaaatgaaaa aaatgaattt gtaaaaatac aatctgattt
    47221 gcgcaaaagg gttgtttctt ttttagaagg taaagtagaa ggtgatgcaa ggtttgcaaa
    47281 ggtacagcaa cggtacggta tactatttga tgcaaaaata tttaaagaca aagaatttat
    47341 atcaacagcc tgtgatgata ttgaaaaaga tgctatagaa gcttttaaac gatttgctac
    47401 ctattttaca ggtttccatg aaaatcgtaa aaacatgtat tccgctgatg aagaatctac
    47461 tgccattgcc tatagagtaa tcaatgaaaa ccttccgcgt tttttagaaa ataaagcaag
    47521 gtttgaaaag atacaacaca cagttgatag taaaacgctt aacgagatag caacagaact
    47581 taaaccagta ttggaaaaga ataagcttga aaccatattt acgctgaact attttcaaaa
    47641 tacactatcg caggctggaa ttacctatta taacaccatc cttggtggta aaactaaaga
    47701 aaatggcgaa aaggttcagg gcttaaatga aattataaat ctttttaatc agaaaaataa
    47761 ggatactatg ctgccattat taaaaccact gtataagcaa attttaagtg aagagtattc
    47821 cacatcattt accatttctg cttttgaaaa agataatgat gttttgcagg caataggttc
    47881 tttttgtaat gattgtattt tttatgcaaa aaataatgtc aatggcaagg catacaacct
    47941 tttgcaaacg gtacaggcat tttgtaacag tattgatacc tataacgata acaggcttga
    48001 tggtttgcat atagaaagaa aaaaccttgc taccctttca catcaggtgt atggcgaatg
    48061 gaatatactg cgggatgcat tacagataca ctatgaagcg tatgaacaaa aggataatgg
    48121 caataataat aactatctgg aaagcaaaac cttttcgtgg aaagcattaa aagatgcatt
    48181 aacaacatat aaatcactgg tggaggaagc acaggatatt gatgaaaatg gatttatagc
    48241 ttacttcaag gacatgaaat ttaaagaaga aattgatggt aaaacaacca gtattgattt
    48301 aattgaaaat atacaaaccc ggtataaaag tattgaaacc atattgcagg aagaccgcaa
    48361 caacaaaaac aatcttcatc aggaaaagga aaaggtagct acaataaagg gctttcttga
    48421 ttcggtaaaa tacctgcaat ggtttttaaa ccttatgtat atagcaagcc ctgttgatga
    48481 taaagattat gatttttaca atgagcttga gatgtaccat gacacattgt taccacttac
    48541 tacactgtat aataaagtac gcaactatat gacacgcaaa ccatattccg ttgaaaagtt
    48601 taaattgacc tttgaaaaat caactcttct tgatggatgg gataaaaaca aggaacgagc
    48661 caatcttggt gttatactaa ggaagggtaa taattattat cttggcatta tgaacaaaaa
    48721 atacaatgat atatttgata gtatccccgg acttactacc actgactatt gtgaaaagat
    48781 gaactataaa ttgctgcctg gccccaataa aatgttgcct aaagtatttt tttcaaaaaa
    48841 aggtgtacag ttttacaaac cttcacagga aatcatccgt ttgtataata ataaggaatt
    48901 taaaaaggga gataccttta ataaaaatag tttgcacaaa cttattaatt tttataaaga
    48961 atccattgca aaaactgaag attggagtgt attccagttt aaatttaaaa acacaaacga
    49021 ttatgccgat ataagccagt tttataaaga tgttgaaaga cagggctata aaattagttt
    49081 tgataagatt gattgggaat atatactatt gctggtagat gaaggaaaat tatttctttt
    49141 caagatttac aataaggact tttcacccta tagcaagggt aagcctaacc tgcataccat
    49201 ttactggaaa aatatttttt cccatgataa tttaaacaat gttgtgtata agcttaatgg
    49261 tgaagccgaa gttttttacc ggaagaaatc catagagtat ccagaagaaa tactacaaaa
    49321 aggtcatcat gtcaatgagt taaaggataa atttaagtac cctattatta aagataaacg
    49381 ctatgccgaa gacaaatttc ttttccacgt tcccattacc atgaactttt tatcaaaagg
    49441 ggaacctaat ataaatcaaa gggttcagca atatattgca tctactagtg aagattatca
    49501 tattattggt attgaccggg gtgaaagaaa tttactgtat ctgtcgctca tagatgcaac
    49561 aggcaaaata ataaaacaac tatcgctcaa taccattaaa aatgaaaatt ttaacacaac
    49621 aatagattac catgcaaaat tagatgaaaa agaaaagaaa agagaagaag caagaaaaaa
    49681 ttgggatgtc atagaaaata ttaaagagct taaagaagga tacctgtcac aggttgttca
    49741 tcagattgca aaacttatgg ttgaatataa agcaatcctg gtgatggagg atttgaatac
    49801 cggttttaag cgtggaaggt ttaaagtgga aaaacaggta tatcaaaaat ttgaaaaaat
    49861 gatgatagat aaattaaatt accttgtgtt gaaggacagg caggctacac agccaggtgg
    49921 aagcttaaaa gcatatcaac ttgcatcgtc attggaatcg tttaaaaaac ttggtaaaca
    49981 gtgtggcatg attttttatg ttcctgcagt gtatacaagc aagattgatc caacaactgg
    50041 tttttacaac tttttaaggg tagatgtttc aacattgaac agcgcgcaca gtttttttaa
    50101 caggtttaat gcaattgttt ataataatga gcaggattat tttgaatttc attgcaccta
    50161 caaaaatttt gtttctgaac cgtcattgca aaaaaatgta aaaagcagca aaatgcacga
    50221 gtataacaat ttaaaagata ctacatgggt actatgctct actcatcatg agcgctataa
    50281 aaagtttaaa aataaaagcg gttattttga gtataaacca gttaatgtaa cccaaagctt
    50341 aaagcagctt tttgacgagg ctggtattga ttatcaagca ggggctgact taaaggaagc
    50401 gatagtaacc ggtaaaaata ctaaactatt aaagggtctg ggcgagcagt tgaacatact
    50461 attagccatg cgatataaca atggtaaaca tgggaatgaa gaaaaagatt atattgtttc
    50521 tccagtaaaa aataattatg gaaaattttt ctgcacgctt gatggtgatg catcattacc
    50581 tgttgacgcc gatgccaatg gtgcgtatgc catagcattg aaagggctta tgcttgttga
    50641 aaggatgaaa agcaataaag atataaaagg gaggatagat tattttattt caaataatga
    50701 gtggtttaac tatttaattg caaagaacac attaaataaa tcaaaataat taatcgaaaa
    50761 tgaatatatg gaaccatata ttcccatttc gatgctgaat gatttcatat tctgcccacg
    50821 ctccatatac tttcatcagg ttcatggtgg catggagcag gatatgtatc atactgaggt
    50881 tcaaattgaa gggaaagcag cccaccaatc catagaaaaa aaatattatt ctacacaaaa
    50941 agatgtactc attggcattg atgtatattc tgaaaaatat aatgtagcag gcaaaataga
    51001 tatatttgat gtaaaagctt gtaaacttgt tgaacgaaaa aataaagtgg taacaattta
    51061 tgatggttat gtttttcagg tatatgcaca aacatttgca ttacgcgaaa tgggctatga
    51121 ggtaaaaaag attgtaattc atgataaaac gcacaataaa aattatccca taccattgcc
    51181 cgaagatgat gatgcaatgc tcaacaaatt tgaaaagctt atagatgatg taaattcctt
    51241 tgacttacat gataccggat ttaacgcaaa cattgaaaaa tgtaaacgct gtatatattc
    51301 tcatttatgt gattactgct tatgttaagt ttaccagatt ttgaagaaaa aagtattatt
    51361 gtatgcttta caacagaagg gcaaacggtt tcatttaaaa atgataatgt ggttataaag
    51421 gataaaaaag ggaaaaccat tttgcagcac tcgtgctata gactgttttc gctatggatt
    51481 gttgggcata caactattac atctggtatt cttcagcgca gtaaaaaatt tgggttttca
    51541 atctatttgt tttcatatag ttttaaaatt accggtgtgt ggaattcgtg tgcggaaggt
    51601 aattttttac tacgcgaaaa acaatacagt tataataatc tggatattgc atggcacata
    51661 gtaacaaata aaataaaaaa ccagatagca ttattaaaaa gtatacgcga taaagaagaa
    51721 gaacaaaaaa gctccattgc caatttagat gtgtatttac accatacagt agagccatta
    51781 gatttaaaaa tgattttggg gattgaaggc attaccacta aattattttt taaggagtgg
    51841 tttaccaata ttgcatggaa tggaagaaaa ccgcgtacca aaattgatac aacaaataca
    51901 ctgttagata ttggctacac atttttgttt aatatcatgg atgcaatgct caacctctat
    51961 ggctttgatg tttatagagg tgtatatcac agaaattttt atcaacgaaa atcactggta
    52021 tgtgacatgg tagagccatt tagatgtatt atcgataaaa aaattaaaaa tgcgtataac
    52081 ctgggacaaa tcaaagctgc tgattttact gaaagcaggg ggcaatattt tttaaaccat
    52141 gataaaaaca aagaatatac cggctggcta ttacaggaga tcatgaagta taaaaaggat
    52201 atgtttgtgt atgttcaaaa atactataga gcttttatga aagaaaagcc aataaaggat
    52261 tatcctgaat ttttgataaa ggaataaaat aacaatgcta cttgtttcgt atgacataag
    52321 caatacaaag ttgcgaacta aattttcaaa gtttttggaa aaatatgggg aaaggataca
    52381 gtactcagtt tttgaaataa aaaacagtga acgcgtgcta aaaaatattg aaaccgagat
    52441 taagcagtat tttgaaaaaa aatttacgca aagcgatagt attttgatat ttcatctcag
    52501 cgaaaaatgt accataacca ggtatggata tgcaaaaaac agggaaagcg acatactcat
    52561 tgtatgattg caaacccccg tcttaatgga ataattgctc atgaagcgat aatttttgat
    52621 ataaaagttg taaaaatcag ctcattttgt gcacaaagta cacaattttt accccaaaaa
    52681 ggcttgtaat gataaaatag ttgacaaaag agcaaattac ctgtctaaag gtattataaa
    52741 atttctacta ttgtagatag aacactggcc attttactgc cactgtgtct aaaggtatta
    52801 taaaatttct actattgtag atgtttgtga ttaagtggtt aactaaagag gtctaaaggt
    52861 attataaaat ttctactatt gtagatatat tgacgaagta tattattttt gtaaagtcta
    52921 aaggtattat aaaatttcta ctattgtaga tatagtaaac tctttccttt cttggaagtc
    52981 taaaggtatt ataaaatttc tactattgta gatctataac tccttcttca tttcctaatc
    53041 ggtctaaagg tattataaaa tttctactat tgtagattga aaaacttaag cttgaagaaa
    53101 ggatgtctaa aggtattata aaatttctac tattgtagat tgatatcttc agaactttcc
    53161 aatatcggtc taaaggtatt ataaaatttc tactattgta gatatacctg ctgtagcaga
    53221 tccttcttca agtctaaagg tattataaaa tttctactat tgtagattga aatataagca
    53281 tcatataatt tccaggtcta aaggtattat aaaatttcta ctattgtaga ttttcgattt
    53341 ccagattttc gatttttggt ctaaaggtat tataaaattt ctactattgt agatattaaa
    53401 tgcttgacat tttcattata gtctaaaggt attataaaat ttctactatt gtagatatcg
    53461 cagtgtgcac agtacagcat tgtgggtcta aaggtattat aaaatttcta ctattgtaga
    53521 tatcgcagtg tgcacagtac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53641 agcattgtgg gtctaaaggt attataaaat ttctactatt gtagattata atttcgttta
    53701 gcgtaatatc aaaggtctaa aggtattata aaatttctac tattgtagat ttcatcgtga
    53761 catgatacga gatgaggtct aaaggtatta taaaatttct actattgtag atcgcgtatt
    53821 acaaagtcat accagcataa ggtctaaagg tattataaaa tttctactat tgtagattag
    53881 atgctcatgg gaagttacta cccgtctaaa ggtattataa aatttctact attgtagatt
    53941 tagattattt ttgtggttaa taattgtcta aaggtattat aaaatttcta ctattgtaga
    54001 taataatcat tttgtttttt agcattttgt ctaaaggtat tataaaattt ctactattgt
    54061 agatatgtca ggtttacgta tcatacaaaa cgtctaaagg tattataaaa tttctactat
    54121 tgtagatctg tatcgtatca attgccatca tacaaagtct aaaggtatta taaaatttct
    54181 actattgtag ataagatgtt tgggattgtc aaaaataatg tctaaaggta ttataaaatt
    54241 tctactattg tagattatca actaattctc tattccattt taagtctaaa ggtattataa
    54301 aatttctact atttgctaat tttggcatca gcaaaattca attctatttc cagtcattga
    54361 ttgaatgtat acttattcat gataattttt catcaaatca ctatgagtgt gctaaaaata
    54421 tagaaacaat tgctatcact attaagccaa tattctttgc aatgatatga aaatcatagc
    54481 ataatggtgg gaataaagtg taatcttttt acaatctact tataatacag tttataagca
    54541 ctatcacgta catgcatggg cagcttttcc agcggatatg caccctttac aatctctgca
    54601 atgtgccagt accattcttc gtgattttct acgtacatat attcttcatt cacccattcc
    54661 gcaaagaatg caccatttgt aatatagcgt atatcctgct cctttactat atcggtgttt
    54721 tctaaatcat cacgtatcat aggccattgc cttatataac ctggttcatg gtcaccatca
    54781 gggtcctcta cattatccat tatgttaatt aaatattcta aatcagtcat aaaatttttc
    54841 actaccgtgc agtattaaca aaagagctac ctgtttccat gaaaagatat ttttatacat
    54901 caaacaataa agatttacat ctaacattta acagctaaca tttcagagta tcttgttatc
    54961 cattggatga taatgaaaaa tcattgatta ttaaaacgtc gttataccct tccaattttg
    55021 ttggcagttc ctttctgctg tgcagttgtc ggatgacatt ttcaggaatt gctgtattca
    55081 aacgcttgct ctgtgcaata catacatcaa gaggagtgtc taaaaatatt gctccgatgg
    55141 tctttttagt ttcttttgct atgtttacaa aacgctgacg ggatttcttt gtcataaagg
    55201 tgttgatgat aagtacattg cgtttaatct gcaaaaaatg ttctacaacc tttctttcta
    55261 catgttttgc cagcacatca tcttcttcat taaatttttc agcctcccag ctttcaccaa
    55321 aatgagtcat ctcgtacatt agcttgcgca gctcaaggcg tgacacgcgg tatagccccg
    55381 aacccttaaa ataggtgctg gcaaattcag ttttacctgc accataaagc ccgcataata
    55441 aaacaatgtt gtatgtctta aatttttcta acgattcata atatttcatt tataataccc
    55501 ctgtttattg tttacagtaa tggtggtgcg atagctcaat gcaatcatca acttgtgaat
    55561 aaatcaatca caatttgcta tcatataagc agctattata cggtaacact ataatcagta
    55621 tcggttatat gatgcttata attaaattat gagtgactat cgccatagcg agcctttgca
    55681 atgcacatat ccatgttggg tagcagggtg ccgttttgca ttctcccttt aagcggtgtg
    55741 gcattgtatt ctttgatgat accggggttg cccgatgcgg tagcggggta ctcagcatat
    55801 tcaacaacaa acaacggggt tttatagtta tgtgcgcttt ttgctgcctc aaaactaccg
    55861 ccgtctggtg tggtttcaat gattatcagg gcattggcca tggcagcaat gaggctattg
    55921 cgcttatacg aatggtgaat ggcatattcc tgagctggtt caaatggcga tatgaatagc
    55981 cagcgggaaa ggtccacaca gtcctgtaat atctgcggga ttgacagatg aagcaatccc
    56041 atgggaagca cagcgatggt gttgccgccg ccttcaattg caccgcggtg tgccatgctt
    56101 tgcggacctt ttgccatgcc actggttacc gtgatggaat gttgtgctgc aatgtgtgct
    56161 gcctggtagg caatgtagtg accacgctcg cttgcagtgt ggtcggatag tattgctatc
    56221 tgttttgatg tgattacatc gtaattccca tacatataca gtatggcagg aggatattcc
    56281 attgaacgca acaaacgctg cgggtagcgt acatcaaaaa ataaaatggg ttcaatagtg
    56341 ttatcaagca attgctcgta ttctttttgt gctgtgccga tagttgatga tacattggca
    56401 acaagctggc ataattcaat gggaagtcct gtttcaaaag ccatcgtttt tggcgatagt
    56461 tctacgatat catacagaga aatattgaga ttggacaatg cagtataaat ttgtttcagc
    56521 gccacaatac ccacgccttg aacacgagac agtgccatcc ataattttgt atcattcata
    56581 gtattgtcga tgagtttatt attataaaat gaatttactc atggtaaaac aagctataaa
    56641 atcaacactt ttttattttc ttatttcaat acgttataag ctggccatgg gcaacgtcta
    56701 cgcaccattg tcattctgag aaagcgccag cgactgaaga atctatttgt tcatcaaaca
    56761 gcgagattct tcacttcgtt cagaatgacg gatgacataa ccccccttgt gttcccccct
    56821 ttataaaagg tgggaatggt gtaaagagcc ttgataacta ttcatacttt cttttgtggg
    56881 gcgacaaaag tttaatcatt tcgtaagggg atatttatgc gctaaatacc cccttaccaa
    56941 cccccaaagc gcacctcaaa cggcggtgag ccttattttc tgtataataa tataactttg
    57001 ccatcgtgct gcggaagaca tcctgtctat gtcctcgcac gagccgtaga gcgctacggc
    57061 atccgtgcct ccgctacttt ttcatttttc ccttgtgttc ccccctttat aaaaggtggg
    57121 aattgtgtaa aaagccttga taacttctca tactttcttt tgtgaggcaa caaaagaaag
    57181 tatcaaagaa aagcgccttc tggatatatc cgttccacgg cgggcgcaca acgggcttcc
    57241 tcgcctgcct tgtcaacgat gtcgtgtaat ggttgtctgc ggcaggctgc cctatcatgc
    57301 gcccgcatcc aggtgcgccg tgcatccgtg cacggctgct tattgttaga atgacaacag
    57361 tggtgaatta ttgttgttta tacaattatg ggattctgcg cctgcgctca ggatgacaga
    57421 cgatcccccc ccttgtttaa agtgggtaaa actatttttt tattgcaaac agtgcataga
    57481 cggtataaaa cagctatgaa taataattat ttatccaatc tgggggaacg ttttatggga
    57541 aagaaaacca tactggtagc tctgctgggt atattgatgg tagcagggag cataccacaa
    57601 acagcaacgc aggcgcagca ggaaaagaag aagatatata tatccggtat aacggtagag
    57661 ggtgcacctg catccatggc cagggagata cgcaacatca tagaggtaac gctgtacgag
    57721 tatggcagtc agtaccgtgt tatcaccgat gatg
//
LOCUS       DAOOJH010000006        57482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_640_length_57482_cov_10.671862, whole genome shotgun sequence.
ACCESSION   DAOOJH010000006 DAOOJH010000000
VERSION     DAOOJH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 57482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57482
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_57482_cov_10.671862"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1818
                     /locus_tag="PLJ75_01585"
     CDS             247..1818
                     /locus_tag="PLJ75_01585"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015477984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPD03941.1"
                     /translation="MAKNNEHILAIDAGTQSIRAIVFNLQGNIVDMVKTPIEPYFSNY
                     PGWAEQEPEYYWKTLCQTCKKLLARGKVKKDALRGVTLTTQRDTMINLDKNGKPLRPA
                     ILWLDQRRADKSIVKLPFYMKAGLRLVNMLDAVEYFITESEANWIRQYQPEIWEKTYK
                     FVFLSGFFTYRLTDDYVDSTGCQVGFVPFDYKKQSWAPPADFKWRLFPMNKDNLVRLV
                     KPSEVLGYITKKAANATGIPQGLPLIASAADKACEVLGSGCLTPNIACLSYGTTATIE
                     TTNTRYVEIIPFFPPYPSAVPGAYNTEVIIYRGYWMLSWFVKEFGAREEMQAKKMGIK
                     PEALFDRLVKEIPPGSMGLTLQPYWSPGIKIPGQEAKGAIIGFGDVHTRVHIYRAILE
                     GLAYALREGAERTQKRNKVPIEKVRVSGGGAQSDVAMQLTADIFNLPAERPHTFETSA
                     LGAAIDAAVGLKLYPDFKTAVAAMTRVGKVFYPNPKDVAMYDALYKNVYQKLYIRLRP
                     LYEEIREITGYPEKR"
     gene            1927..2130
                     /locus_tag="PLJ75_01590"
     CDS             1927..2130
                     /locus_tag="PLJ75_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03942.1"
                     /translation="MEIVSGILCEVCHENEAVVRCDGCGKALCKKCRLFDIWCYGCGH
                     GDTKAFCKQCNENPAINIWKTPV"
     gene            complement(2135..3055)
                     /locus_tag="PLJ75_01595"
     CDS             complement(2135..3055)
                     /locus_tag="PLJ75_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD03943.1"
                     /translation="MAIPTSRGNAITTAGIDEKSCTGCELCVTACPEKNIFIKDKKAA
                     FQLNPYFGCIGCGHCMAICPKGAIAIHGRDIDASNVFPLSYTTNAATYAQLLSLMQKR
                     RSTRLFKDTPVSKDSINKIIKAAQTAPMGIPPSEVNIIVWESKESVLTFTKDFCSLLE
                     QMNWMVSDWFLFIMKIFLSKTNYLFFKDFVKPLVNIYVDNMKHGINYVTYDAPATIYF
                     YGSPYCDYADPIIAATYAMLAAESLGLGTCMIGGIHPFLQNGSKAKAFREKYGITYKS
                     KTGLFVIFGYPKIKFKKGIKRSFASVTTIP"
     gene            complement(3068..4276)
                     /gene="icd"
                     /locus_tag="PLJ75_01600"
     CDS             complement(3068..4276)
                     /gene="icd"
                     /locus_tag="PLJ75_01600"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HPD03944.1"
                     /translation="METIKYENGIIEVPDNPVVLFIEGDGTGPDIWKATQIVLDAAVN
                     KAYNGKRRIHWQEILAGEKANTSTGSWLPDETLEKIRQYKVAIKGPLTTPVGGGIRSI
                     NVTLRQVLKLYACVRPVRYLTGVPSPLKKPERVNMIIFRENMEDLYAGIEWKQGTPEA
                     KKVIAFLNNEMGQHLSDDTGIGVKPISMSNTKNIVKSALRYAIENKRKSVTIMHKGNI
                     MKFTEGAFRDWAYQTAQEEFADYIITEDELWSTYQGKQPAGKIVIKDRIADALFQMVL
                     LRPDEFDVIVTPNLNGDYISDALAAQVGGLGMAPGANIGDDAAIFEATHGTAPKYANQ
                     DKVNPGSLILSGEMMLRFMGWTEAADLIIKGLETTIQQKRVTYDLERQMEGATLLKCS
                     EFGKAVADNM"
     gene            4665..5582
                     /gene="trxB"
                     /locus_tag="PLJ75_01605"
     CDS             4665..5582
                     /gene="trxB"
                     /locus_tag="PLJ75_01605"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPD03945.1"
                     /translation="MKELIIIGAGPAGLAASIYAVRSGLDTLVLEKISPGGQVMLTHA
                     IENYPGFIEPVEGWQLAYNMEQQARRLGVAIESKDVVSVEKYDDMFHVKTTDGAVIEG
                     RAIIAASGASLKRLGVPGESEFTGRGVSYCATCDAAFFKDRVTAVVGGGNTALEEALY
                     LTRFASKVYIIHRRDEFRGQKILQDRVLSNPKIEPIFDTLVTQVHGDEKVYSITLQNK
                     KTGESRELAIQGLFIFIGNTSNTTYLPEQVLNDSGEVKVDMKMQTPISGLFAAGDLRE
                     NSLRQVLMAAADGATAAISAYEYITHKDW"
     gene            complement(5623..6402)
                     /locus_tag="PLJ75_01610"
     CDS             complement(5623..6402)
                     /locus_tag="PLJ75_01610"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HPD03946.1"
                     /translation="MEKPYCFIIPHGGYQIPEELAQLCVLNEFELFFEADTCANDLFD
                     LQQIGTVIHTSIHRLFIDVDRDPYTLPPRSSNGVMKMHTSQKKLMFTSGVFPDEIAIA
                     AMMKRYWFPFHNSIEHTVKSTRCIIVCHTTMPVAPIVARDAGIPRPIIAIEHGTTNGS
                     DIITCEENTALKLLSFFQKAFGSEDCTVAAPFVIAKPAQRYIMQRYAKHQIPIIRVAL
                     SKALFLNEKYFDPHSLSVDSNRIQHIQERLHDCFSKFARYI"
     gene            6545..7051
                     /locus_tag="PLJ75_01615"
     CDS             6545..7051
                     /locus_tag="PLJ75_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPD03947.1"
                     /translation="MRPYKIDTLGEDILEILSHDAKATVEDIARQLDVSIDKVKKSIS
                     RLEKDRIILRYKTHINWQRVKHDEVRALIEVKVTPQRGVGFDSIAEVIYRFPEVTSVY
                     LLSGGYDLLVQVEGPSLREVAMFVAEKLATIDHVQSTVTHFMLKKYKEDGDILVEQPE
                     GKRLPVSF"
     gene            7058..7711
                     /locus_tag="PLJ75_01620"
     CDS             7058..7711
                     /locus_tag="PLJ75_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPD03948.1"
                     /translation="MFSLNTVYQKLFACYGPQHWWPGETPFEITIGAILAQNVSWLNA
                     AKAIASLKAHNLLTPHKLFKEDSVAIAHHIKPSGYYNQKAFAIQSFLQWFHRYDFSFD
                     ALNAIPTELLRKELLSIKRIGYETADSILLYALHRKVFVVDAYTKRIFTRAGFLTGNE
                     DYMAIQAMFHEHFTGNTYDYNEFHALIVKHGKDRCTKKPACNECCIADECKEYDVAK"
     gene            7976..8146
                     /locus_tag="PLJ75_01625"
     CDS             7976..8146
                     /locus_tag="PLJ75_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03949.1"
                     /translation="MNTNAPKSATWWIAVIIGVLGIVGKFVHISVLEAYSWWLVCIGF
                     IVLALATAIKGL"
     gene            complement(8191..8925)
                     /locus_tag="PLJ75_01630"
     CDS             complement(8191..8925)
                     /locus_tag="PLJ75_01630"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HPD03950.1"
                     /translation="MIQKSLRWIALIASLCFFSNPGGYPIKTIPYYSLYTLARASAPA
                     PITVSHSKPGMPAIIRGTLITYKNRNARKVYIAGDFSYWKRQPMERSNNGVWFYIIPF
                     DDEERIIRYKLMVDGIWINDPVNMDIDDDNTGSYYSIVRQDKLPESKQISFHIVNEST
                     RLVEFRVYAPHATIVALAGDFNNWNTEADLLKKDKNNIWRLQKRLPEGVCRYNFVVDG
                     TWQLDLYNPHTASNAAGMLCSVISLP"
     gene            9122..12124
                     /locus_tag="PLJ75_01635"
     CDS             9122..12124
                     /locus_tag="PLJ75_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03951.1"
                     /translation="MAVSEEIKNLTPEEEAEVKQIVDAFDSELSSIAQMRGKIGILHA
                     DSTDADFTITPGMRSKEMETTSAPVEGFEETEDIVDITDMIEEVPEDVVLASEAVLPE
                     VEETIESVEELPSEAVEIAPEEVLPPEFKEEFPAEEVPERKPLKKEAISPLDELEELT
                     QFEPETVEAHDIRTDEFVTEEPRIHGDVHLDEDIHLSELETEEISEISEIKAEELPQV
                     DISTMGMEEEMPEPTAFEEEIKEEPARARFETPSFDELDTFEEPLKKSEKIESVIDEF
                     KPEFEGLSVEEEEPAPIEEGVEISDAELRRLKKALLLLHPALRKKIKEIILRDELSPQ
                     DTRKLIDLIISGKPEENIQRFIEKRLNITLELEEEVPSHRRVITARPEYTREGMERQK
                     RLLRQTKTLALAVVVGFTLTVTVYQYIYKPFMARRLISKGVALILEPGDPATKKVKDY
                     KKAEQLFEDVDAHYKKDYLYGYNAYARAYFKKREFDYAYVKLKRAYSINNRDVETLNN
                     TGYFFAKIPEHYYNRIQNDAIKTFAPKDTYVISQVDLAIALYNKVLALDPNNITAMFG
                     IGNAYLYQGQYLKSRQYFENIIKVNPDSWIGYAGLLNLFIERDNLPEVLSIHTDLRAR
                     DILTDIPSPLLAKLAHYYLGKNRSDTFNVRVDLGVQSPRLKDEEDNLIPACRGVLDAL
                     KEKDPDYPPLYVASAKLAAAQKNLKLTQMLLQEAEEKAQEKGERYFAALHLLGEYHYT
                     IKQPVEAYKYFNAALTAYQYPPEFTYEDFYYESETIGKTYAYLGNIFYYFFDKVKFRF
                     GDELDEALLDEDSDKMANLDIARQKYELALQENYSSPELRYNLGRIFYINQNYQQALE
                     QWINLYEDIVTNPQVMLAIGNAFYHMNNLEASKGEYLKMIAVFERKAESIRRPMPERN
                     EHIIIFQSLASAYNNLGAVYQIQGNETKSSIAYWKAIEYATRIGRENEFARVNLARGF
                     KPRVTPIMPILDESIPYSIDIYREDMRK"
     gene            12143..12274
                     /locus_tag="PLJ75_01640"
                     /pseudo
     CDS             12143..12274
                     /locus_tag="PLJ75_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
     gene            12416..15160
                     /locus_tag="PLJ75_01645"
     CDS             12416..15160
                     /locus_tag="PLJ75_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD03952.1"
                     /translation="MQTVKAYHADAVMQYDDNAIIEHIAQHGFATDSRFLLKTESYVL
                     SCAYANNKMLSLSNSRTRILPHQIESTYTVVNALHKRFLIADEVGLGKTIEAGLIIKE
                     LFYRFNYERILIVCPASLLYQWQYELKSKFNEDFIIADSRVYRSEKGNPWKLNKLLCS
                     IDFIKNKHFAGKLSQTYWDTVVIDEAHRLRRDANSQTKSYQVAEILAKNSKALLLLSA
                     TPFRGKLEELYYLLSLLDKNLLGPIHSFQETYCVPEADLTLLRDTISKVIIRRTKYEV
                     GGFTKRLAKTIRFELYPEERELYNATTEYVADQFNKAMQTNNRTIGFVMTVFQKLLDS
                     SSYALLCALQKRYQYLTEIYNNHHHAIQSMQEIDENVIEAEDVERIVQVESAELENEM
                     NTLATLITMAQSIVRSKKGEKLLQLVRKLFAEKHNKILIFTQFRTTQDYLAQLLSHYK
                     VVLFHGSMSRDEKETAIEYFKSEAAILICTEAGGEGRNLQFCNVLINYDLPWSPLKVE
                     QRIGRIHRFGQSSNVIIYNFATRNTVAERVLEVLQHKLKLFEESLGTPDIILGDITDE
                     RFFNNIFMELIAGLKNKKRVAKDIELHIQAAKASYEKMGQLAVTKKIDLNFDEYYTIT
                     LQERDFSNKRIENFVNTLKNIEPTAVDFLSVQNKKTRFYGVHHNPFTLQPVNAQGTFD
                     SAVALENESLEFLAFGHPVVDWCVEHCKEYTFGGLSGIKQIQYSYDCSGIVFNYLLTI
                     HAKETMQRIVPVFIPYNTMDDAADDATIEDIASESMRQFGIVSSVVDDAVVKYYQHNI
                     YSCYNQSRKVLDQKIHHIIAGIKESMLLEVDPYTERVEDHYSKEIAHVKQRLEWQECQ
                     QRWYDRDMHSAITRSRNTIKNLEEEKLQQLQQYKNFITIQWDAKLINAGLLICKQQLP
                     "
     gene            complement(15152..15466)
                     /locus_tag="PLJ75_01650"
     CDS             complement(15152..15466)
                     /locus_tag="PLJ75_01650"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD03953.1"
                     /translation="MAEKRKYNRVQKKCKSVVESMEGVTYSTINDISEGGIFISTPEP
                     IHPGSTVDVELCLSPEEKITLKGTVRWIRIKEEDNKKAGMGIQFIPTHKEIEKLKKVI
                     SR"
     gene            15745..16344
                     /locus_tag="PLJ75_01655"
     CDS             15745..16344
                     /locus_tag="PLJ75_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPD03954.1"
                     /translation="MHIIYLSKKVLFPHCTITVELKRSRFLYTLKPGDKVIVYPVKKI
                     ADFILMQGVATLAEIVKSELSNNKYTITFKGIKRVTIDKRHGIFQCSFTEIHERAIVT
                     NEVTEQIRKKSQELIFLINVDESDKLIHLLNFISDAVQLSDFIANYFILDYDKRVMLL
                     NNCDVSSRIDVILALLNDLIKNVKQKREKESYEKENISG"
     gene            16319..16783
                     /locus_tag="PLJ75_01660"
     CDS             16319..16783
                     /locus_tag="PLJ75_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03955.1"
                     /translation="MKKKIYQDEVLYTLAWSPMYQFDKYSITKIIPELAGIVALMEKR
                     SEETYHYILFYSCWRDGMRLGLKNLFDPIFTRHPDLAKAMEGRQLWYKYTVVDSNPFD
                     LQDILWWLIQNYEPELNDIQNYEDSKRYKNIYVREMELDRETVVERLPKYGF"
     gene            complement(16780..17379)
                     /locus_tag="PLJ75_01665"
     CDS             complement(16780..17379)
                     /locus_tag="PLJ75_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03956.1"
                     /translation="MDEEKQIKDIESLLRERKPLAEIAQYILEGAFVELDMERKDSLD
                     RFLEFVYSKVQRGNPYIIHLAYPTKRMTDTELETKVKELINVHLNPDIVLPLLKIFTR
                     NVHNADSNLYIAYLIEADNIIKSIFDTYLLFKKDIFETDKEKRTQNVRRMQQFLAHIE
                     SESASPLDAAARLKYILEFIALKQNVKHIYTMDDIALSH"
     gene            complement(17383..18234)
                     /gene="bamD"
                     /locus_tag="PLJ75_01670"
     CDS             complement(17383..18234)
                     /gene="bamD"
                     /locus_tag="PLJ75_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPD03957.1"
                     /translation="MKRCMILFFIFMVHAQQIDAHPLLELADTFMNNKEYYHAITECM
                     RYQFLFPDGKEYPQSMLIMSRAYFLGGNYYRATDIAYDCYKKYTGTMYGDSALYNLAV
                     MRLMEGSPFFAYRTYQEYFSIYPDGKYRETARLDFIYAQILLNNIHDAKNNVDAFIKQ
                     TSDEELRNEAMACLQLLHDYEKRPQKNIWVSATGSLLVPGFGHFYAGKYTVGVLSFVS
                     TTLCAALAYRGYTHDNNFQLIFFGLAGFTFYQYSLVSAVLNVGEYNSNSINQLKKAIH
                     ITVQKRF"
     gene            complement(18231..19055)
                     /locus_tag="PLJ75_01675"
     CDS             complement(18231..19055)
                     /locus_tag="PLJ75_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03958.1"
                     /translation="MSALLLLTLFFVHPDDAFVHKQQQLISTLYYTANYYQCIAESRR
                     YSMLTDDNMDYTIALCYCKGNQLATAVAILQKKEKKEYCDYLLLSKVYTSLQHYQDAL
                     QALSLCDTSAQDKPLQMLYYQNILAVYINRYDWDNALNVYTEHSDRLQQMHQIYTILN
                     AAQKEKINPYVAVTLSAIIPGSGHCYARHHADGLISFITVAVLSAATYIAYLNGSFAL
                     TCTLGTLTAIGYAGNVYSAYNSAIHTQETINNNYRTELNRYLEYNPEQFLPQWMKK"
     gene            complement(19052..19294)
                     /gene="yidD"
                     /locus_tag="PLJ75_01680"
     CDS             complement(19052..19294)
                     /gene="yidD"
                     /locus_tag="PLJ75_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPD03959.1"
                     /translation="MHNAAGAIGYFFIRMFQVVISPQDGPNCRYRPTCSEYGRQAVMR
                     YGLVMGGFLAGDRIIRCNPFAIPNHDPVPQTLAEHP"
     gene            complement(19456..20076)
                     /locus_tag="PLJ75_01685"
     CDS             complement(19456..20076)
                     /locus_tag="PLJ75_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD03960.1"
                     /translation="MIIEQLLVSPMDVFCYLLVCPITKEAVLIDPAADFDTIMQAIRK
                     HNAHVKYIINTHGHFDHTSGNNTMMKLTGAELLIHENDVRQLTGITNRIMSHINRGSA
                     SPKPLHTVKEGDTIIFGNEKLTVMHTPGHTPGGICLYSPGNIFTGDTLFTEGMGRTDL
                     PGGSYQQIMESIYRILSLPDDTIVWPGHHYGRFPKSTVAEQKRYYM"
     gene            20708..21547
                     /gene="asd"
                     /locus_tag="PLJ75_01690"
     CDS             20708..21547
                     /gene="asd"
                     /locus_tag="PLJ75_01690"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HPD03961.1"
                     /translation="MKGLPMLLFFKIIPTRLLSRIFGIAAQIPLPQSILKKLIVWYSK
                     KFNVLLEEAHIPPGGFRTFDEFFTRNLKRGMRTIDPDIYSLISPVDAMVSSYGDIVYG
                     QIVQAKGINYDVASLIPGVMESTFIGGSFTTLYLSPGDYHRIHAPVTGTITAYQYDPG
                     RLYTVQDWLVNMLPGLFTVNERVTTYIQSAHGLVAVCKVGAMNVGRITLSYAPLYTNR
                     FCAKPYFYIMDRHKQVTIQKGDELGTFHLGSTIIVLTQKRCRFLPAIAGKKVTMGQAI
                     AFF"
     gene            21676..22389
                     /locus_tag="PLJ75_01695"
     CDS             21676..22389
                     /locus_tag="PLJ75_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPD03962.1"
                     /translation="MKDVAVKESAIFKQIEHWRRQKAVVGDKEKANILPFVTISREYG
                     CGGFDLAVKLSDILNNEYNAMPIWAAYDKQILDTITTDLGLTQKLTDTLTNAARSKMT
                     ELFQTTFSKFPPQVAVYRKLAEVIRTMAINGHVVIVGRAGNIITRGIPYGLHVRIIAP
                     LAYRVERIAAKNKVSKAEAKRIIEKKENERESFIKVFLKFDLEDTHNYDIVVNNERFA
                     IDELARLIIETMKHKGILA"
     gene            22405..22878
                     /locus_tag="PLJ75_01700"
     CDS             22405..22878
                     /locus_tag="PLJ75_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03963.1"
                     /translation="MQQFDCNETSIVYYPEKYIIVCIAYGDGKKVWAKKMHEPVAIQN
                     VLFDERYYYIACKTGDTEGIFLTLDKSNGSTVWFIPGCTSLEVLYRGYLYLIFVDDNE
                     QHFLIKVECDEGNKLWFHQIDSDLYYYHFKEDCIVLHYASGKKELIRYDGKLLLQ"
     gene            22999..23853
                     /locus_tag="PLJ75_01705"
     CDS             22999..23853
                     /locus_tag="PLJ75_01705"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD03964.1"
                     /translation="MKAIGIPTEAASIYNKALALSNMGNYDLAIEQYMRAVEVFPQFI
                     EAYNNIGELYSRMGNSEKAIQVYSKALSIKKNHKVLLNLGVEYYNAADYTIALQYFNE
                     SLLLKKDFLEGNFYTGMTYFNLKNYVSAKKYFEATVAIDAHHLKANYLLAYILYEDKE
                     YLKVIECLKRIEHIADDLMFINRYYGFCYYHLGDYKKAVKFLSAALTNSPKYAQFKAY
                     LESLTYENKLKEIGDIDATIQALERKIMAEKPNLSEYTRLSMLYIFKGQYKKAEDLLV
                     GVKSKIAR"
     gene            complement(23889..24374)
                     /locus_tag="PLJ75_01710"
     CDS             complement(23889..24374)
                     /locus_tag="PLJ75_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPD03965.1"
                     /translation="MVTEGKYVAIHYTGTFDDGQVFDSSLDGTPFEFQIGAGMVIPGL
                     DKGVVGMKLDEEKDIVITPEDGYGDYDENLLYAFPLEEVQQQFKPEIGMTIALQMDNG
                     SQIPAVIKEIGDDEVVVDMNHPLAGKTLHFHIKVINISDSPEYSNGCDACGGSHDGCS
                     C"
     gene            complement(24631..24945)
                     /locus_tag="PLJ75_01715"
     CDS             complement(24631..24945)
                     /locus_tag="PLJ75_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03966.1"
                     /translation="MIFCDTRQTIQIYYTLMIYAQPVNSSQITQELSPILTMLIIQLK
                     PFTGKLPIKKLLIHFTVDENKKPVGGATVFIWNSNEKCFYSQSEAWGPEGTVMSVHKL
                     KK"
     gene            24997..26499
                     /locus_tag="PLJ75_01720"
     CDS             24997..26499
                     /locus_tag="PLJ75_01720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPD03967.1"
                     /translation="MKSIQIGFIGLGRIADLHYLGYTNNKQAKVYAVCDSNLELAQKR
                     KKQWNAEKVYTDYRDLVADAHVDAVEILTPHSLHESMTVAAAHAGKHIALQKPMTVDL
                     KSSDRIIAATDTAGKIFKVTDNYPFYPPIRLAKKIIDNGEIGKPIALRIKFIGGGSGG
                     WHVPPEAWQWRIKENTESGGMRGLDTFDHGHHLWTTAWYLCGGVEKTAGWIDSIDGII
                     DAPAVMMWKYDNGCYGSIEFVHLPSLTIPCKYYANDEWIEVSGTHGIVVIRRCTGSIN
                     KGPVVGVFTNKGWKNYNVPSDWSLGFVGATHNFINAILGKEEPMLSGRDAREILRFSL
                     AIQKSARLNREVYTHELDSKFPSLYYVRHHCKELKAKKPKKSIAERLGLSANFAKYAP
                     QAKELTRQFIKRYNPEAAKQWTVTIALHIKKEGNADEFLYSIAINNGALMVKEGALPE
                     KWDMKITAQAGVWAAILLKKKRIEMAYLQGLLKIEGKSEEALRLRSAFGI"
     gene            26517..27878
                     /locus_tag="PLJ75_01725"
     CDS             26517..27878
                     /locus_tag="PLJ75_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD03968.1"
                     /translation="MKQSIAQTGLSLITKLGYAVGDFGANMVFQSVVLFLMFYLTDVF
                     LIPAGVAGTIFLIAKIWDAVSDPMMGYISDRTKSRWGQKRPYLLFGALPLGICMMLLF
                     YTPALEGSSKTMYALVMFLLVCTAYTVVNIPYGALTANLTLDTNERSRLTGYRMFFAI
                     LGTLFVAGATKPLVGLFDSSVEGWRFIGMLYGAIAAFFTLVTFATVKERIQHKDTDEY
                     HLRDIATTLKVNKPFIFLSIGVFLHLCAIGVLASMVNYLLKYNYAKEEFVPVAFLCMF
                     ATAAVAIPLWVFLSQKMGKKHAFNAGMGMLAVILLVLFFVKEFNSVLFIVLFVIAGIS
                     ISTVFFSPWAMIPDTVEYSQWKTGIRREGILYGFFYFSQKLAAACAGFIIGIGLTLCG
                     YLQPFLVDNTLTSRMQTEGALTGIRLLSTIIPMLLIIVGIVFIWLFPIDEKMHRKIVE
                     EIG"
     gene            27953..28222
                     /locus_tag="PLJ75_01730"
     CDS             27953..28222
                     /locus_tag="PLJ75_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03969.1"
                     /translation="MIYYDKEVQKAIKKSDIPTKIVKRFHNAFVSLDLTKDLNLFDVK
                     KVKGSFRRNYCRLRKGKFRTMFFIENDDFYIVYIGKREEVYDQWQ"
     gene            28213..28422
                     /locus_tag="PLJ75_01735"
     CDS             28213..28422
                     /locus_tag="PLJ75_01735"
                     /inference="COORDINATES: protein motif:HMM:NF039402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HPD03970.1"
                     /translation="MAVTSVRFNAKEEKIVKILKKHYNCDASTLIKKSLWELYEDIKD
                     KIIEAFEKCEKGGKTKFVTIDKMVQ"
     gene            28784..30631
                     /locus_tag="PLJ75_01740"
     CDS             28784..30631
                     /locus_tag="PLJ75_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD03971.1"
                     /translation="MNLKKLIRNYLSFVNFSTMIQLTMHWLWFRRKRVFFIFEGREYT
                     YNEVYKQSKRYATFFLSMRKKLIESGRLHDNQRLAFGVYMDNNPEFIFASFGAGLSNS
                     ILFAINTGFRGETLAKVIDQANISFLIINESTVHEVAPIINNSTVIHADDIYYAGNNN
                     GYAAQGFHSLEEAVDHAQHENIPKNYRTKIDNFSPVIVIYTSGTSGLPKGVPCTHIKL
                     VGAGFIVQFAVHLNKNDRGYISMPLFHSNAWYIGILPQLIVGGSFVLKRRFSASAFEE
                     DMLRYGVTFLNYVGQPLHYIIEALERKYGSGEAVEAALAHHPRNKFRKAYGNGATVTD
                     RIKLKRYLGMEHIYEIYGSTEAVITTANLPGDPIDSVGRVDSSIKILNEEGKECEPGI
                     VDENGALINYDKAVGEICRKVGDSNLRFEGYFGNEKATSQKFRDGWYHSGDLGHIRII
                     NGRRYLFFNGRTDDWIRKDGENFSAENVLDYAHRIPGVDIAIAYGIPSEVSDEKVMIT
                     IQMKEGASFNPDEVYHWFMQQQKEGGMDPKWMPDYIRIIDKFSVTNTQKIMVRPFKKE
                     NFNLDNNPTMKIYFRQRGDNTYRLLTYDEFVKIKEAFKTNGREAVLSRN"
     gene            30791..32008
                     /locus_tag="PLJ75_01745"
     CDS             30791..32008
                     /locus_tag="PLJ75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPD03972.1"
                     /translation="MEQLRSFTSPFDISRCKECGECLHQCPVLQMKPDEAISERKKLN
                     RGRVSAKIMKRCTTCFACNLVCPQGCNPAQQIIDLWHNAYRSKGLPLRARYFDPNHST
                     NFRTYVVDRLPYDERALIEKWKDTSECAEILYPGCNIITAPYLTQTSLLEGLEIRGSL
                     DVCCGETYYRMGLIDEMYKVAERLERYFKKLGVRKMIIPCTAGRNMFTNVLPSSGVKF
                     DFEVVHILPWLWERMEKGEIVIKEKLNMTVTVQESCYGKIFGNDYMDIPRKILNSIGV
                     QVIEEKLIRSHALCCGIAGGFSVKSGYHPVDIAHETFRSLGLAKQTGADAIAVYCAGC
                     LQMLTVGQILYPFNRMPVYHILELLQMAIGEKPARLNKNRAQTLFAGVLKHQVPLLFS
                     LNRHRVDVKKLDF"
     gene            32011..33462
                     /locus_tag="PLJ75_01750"
     CDS             32011..33462
                     /locus_tag="PLJ75_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPD03973.1"
                     /translation="MKQYRWFRDNRCRVCGQCLSFCPVVSLPAGAARRDRIIAREGSI
                     NESLSLSYCTTCNACNVICPTQSHPYELVLERYDEAQRNKGLPGIARLVFPNEYLNIW
                     AITGALLTRKEKHFLKQWENNLHEKHSTIMLTGFYTNLVPFLLDHVMFQNSTIPIAGS
                     EGLWGCGGDAHKLGLVDITEKVALLLKNKLHSMKVKKVICFMEAEAAMLQEILPQYYG
                     IDYNITIIPIEEWLLQQIKQGDITISAPLAIRIAVHDNCMSRYLNGKPQKTMREIVKR
                     CGGLSVEMEHAKEKSLCCGWAATIPSLYRNASLFGIALYLLYSLDKRLCEGVDAKADV
                     MVTGCPACYIFLSVAQYVTRRKIKIMHLTQVVSLAAGQPHNAMMDRRVRDILAGIIFF
                     LLRWISSRSVREHFHPASPQTDDVQTIPVLNKSDAKTLQRIRSILDSPLLTNRITQWL
                     IAMMVRLAVRLYGLCLAFKRKQFIRKQCSKVSG"
     gene            complement(33560..35425)
                     /locus_tag="PLJ75_01755"
     CDS             complement(33560..35425)
                     /locus_tag="PLJ75_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD03974.1"
                     /translation="MNTGIQQRAAAIARHSSLHKAVLQGTIAQYQDITVSEALVLGLL
                     QQGVTRYFGIFGHGSTAIAEVLRIYEYHGLVHTYNLRHETAASHAATMLNMLTGEVAA
                     VVTSIGPGALHAFAGSLASASNGAGVYHIYGDETTHDEGFNMQQIPKDEQALFLKLCS
                     VMGNAYALYEPWSVISALKRGAVCTKGLPFAQPFFLLAPMNVQPAVLTHFNLLQLPHA
                     HRHVPVVCYDDAVFEKATALAKNAKRVTIKIGNGARGCGSYIETLANLLDAAIVSGPS
                     SAGIVPYTNPRYMGVGGSKGSLSGNFAMEADCIIVIGARGVCQWDCSGTAWKNARSIV
                     NFNVNPFHAAHYNRCVSVVGDAQGNLQRWIEHLRANGFSMGDSHSEWFLALQEKKAQW
                     QALKQQRFDCPVLFDEMWKRDVLTQPAAIKTACDFADEHNYIKVFDAGDVQANGFQII
                     EDKHEKQTITETGASYMGFASSSVLVSGIVDKKMMAFCGDGSFIMNPQILIDGVEHGA
                     NATIIIFDNRRMAAITGLQYAQYGSEYKTSDSVAVDYVAMAHSVHGVQGIYGGYTPSE
                     LMQALQKADQYRGLSLIHVPVYYGQNELGGMGVFGSWNVGSWCSQVQEEYHRQGW"
     gene            complement(35591..36217)
                     /locus_tag="PLJ75_01760"
     CDS             complement(35591..36217)
                     /locus_tag="PLJ75_01760"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPD03975.1"
                     /translation="MKIAVINETSAADKNADIMQALQNRGHEIINAGMTISGQSPEIT
                     YIHTGLMAALLLNAKRVDLVVGGCGTGQGFLNAVMQYPAVMCGHIVTPLDAWLFAQIN
                     GGNCISLMLNQGYGWASEQNLTFIFDALFSVQWGNGYPAHRKESQQSSRQLLSAISNL
                     THASMAHIMQALPDEVVKPALEYPGFIDSMDVPSLDDELKHAIVARIS"
     gene            complement(36214..37050)
                     /locus_tag="PLJ75_01765"
     CDS             complement(36214..37050)
                     /locus_tag="PLJ75_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006091262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPD03976.1"
                     /translation="MQKLIITAALTGGIHGKEANPALPEQPDEIIQAAYDCYNAGAAI
                     VHIHARDRDGNQTASKDIFATINNGIKSKCPVIIQNTTGGPGLPVEERIASLDAHPDS
                     ASLNCGSVVFFYQGKQLPFHNYRHEIEAFAQAMLQRTIKPELEVYNPSMFLEVENLIA
                     KNLLALPYYINLVMGVNGMGGYPGTPENFLTMLRHLPDNSICNVSGIGKYQLTMNTMA
                     ILTGCNVRTGLEDNVFYHKGKLATSNAQLVERVVRLAKELGRDIATPDEARTLLRIGG
                     AS"
     gene            complement(37052..39193)
                     /locus_tag="PLJ75_01770"
     CDS             complement(37052..39193)
                     /locus_tag="PLJ75_01770"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD03977.1"
                     /translation="MQKRHPMNNAGFDFKKLHNELIASGNVILPEIESKHIVSRYAIP
                     TPKGKFVTTIAQLKEASTLLTFPVVCKVSGRLLHKTEIGGVITGIKSYDELKECFSML
                     DSSMKQKNIAGYNGILVEEQCHGHIEMIAGYTNDPVFGPTVMLGSGGIYTDLFKDVVF
                     HHAPVTEEDVMSMLSRLRCYPLLMGYRNIPTIDCKPLIQCFMNLNRLICDGARYIQSI
                     DFNPIIVDEKSCVVADASFTFYHAPIPDPFIVEKPAVQHLHTFFNPASVAVVGASTVV
                     NKIGNVIVDTLINGGFLGDIYPINPHQKEVLGLTAYHSINELAKSPDVVIIAVDLQMV
                     PEILDQMHAKGCHNAIIISGGGKELGGTREKLEKEIEAKAKCYDIRIIGPNCIGVFNS
                     LSRFDSFFYHRDRFTRPPQGTISFITQSGTWGVTFMELAQTTGLHIMVSYGNRVDVDE
                     GDLISYLMDDAGTGVIGSYIEGLQKGKKYAEAIAHAIKKHKPVVVYKTGRSDISSQAA
                     ISHTGAYGGSYTLYHDMLQDAGAILTDSLHECFAACMALSMQPPAKGNRVALVSNGAG
                     PMVNALDLFPQKNLVLAPLSDTTKKTMREHFSFFYIVHNPVDVTGSATSDDYDFVLQQ
                     LMNDDGVDIIMPFFVFQNTPLDEAIIEKMKRHSDLQKKPIVCSCTEGTYSQRMNNRLI
                     HAGIPVFHEISHWVVAVYALMQWGKIIQHEV"
     gene            complement(39168..39575)
                     /locus_tag="PLJ75_01775"
     CDS             complement(39168..39575)
                     /locus_tag="PLJ75_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD03978.1"
                     /translation="MFIDFSTINGTTVGRPFERELKVILSPDTNRSINDFTFILSTLA
                     PNGGCTDFHVHDTSGELMIFMSGSGKAWFEGMEYKIKPGTALYAPPGSEHKTMNTGNE
                     PLMIACIFIPAIASEYIVKSAEEANNAKEASNE"
     gene            40016..40777
                     /locus_tag="PLJ75_01780"
     CDS             40016..40777
                     /locus_tag="PLJ75_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HPD03979.1"
                     /translation="MAQLIQSLERGLRILEILGKSDKPLSLGEITQFFDIDKSSIFRL
                     LSTLTQNNFVYQDAETKKYMLGFKIMELSGAFGEQTKIETFLRPVLKAVCDKTRQNTH
                     LAILHGQEVVFIAVEQPRNGISMHISVGTREPAHATALGKVLIAFTQKNEQELLLQGI
                     VFTHYTPHTIVDGDEYRNHLSSIVQNRYAVDDEEYRLGVVCIAAPVFNHRGEVEYSIG
                     ISGPKDQISPHIADYITVVQDAGAECSRLLGMSSR"
     gene            complement(40781..41233)
                     /locus_tag="PLJ75_01785"
     CDS             complement(40781..41233)
                     /locus_tag="PLJ75_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03980.1"
                     /translation="MRILIHLLLISFIVACGTSKPLTPQQALQQLSDSYTSGDMAKVA
                     QLLSTNSKKRIQNAIQLIGSMNQSQLSSFAKHYRISAASLKNLTIPQYLSLQKTIASI
                     EGDDVILQALQQPVTDITINNAQATVTVYNGMTLIFVKEGAYWYFEYE"
     gene            complement(41221..42090)
                     /gene="panC"
                     /locus_tag="PLJ75_01790"
     CDS             complement(41221..42090)
                     /gene="panC"
                     /locus_tag="PLJ75_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPD03981.1"
                     /translation="MEIIHTIDALRDIIKKERTNNKRIGFVATMGYLHQGHLSLVHIC
                     KQHADFQVMSIFVNKMQFNDPRDYDTYPRNYDRDFALAKEAGVDLIFLPDDSEMYQNR
                     LTYIDIEMMTSFLCGSARPGHFRGVLTVVAKLFNIVQTDVSVFGQKDIQQAVIIQKMV
                     KDLNFPLDIIVAPIIRESDGLAMSSRNVHLKGDDRTNATCMYRSLKKAEELIQHGIIQ
                     ANTIIPAMTDIIAAGKPKKIDYISIVDYETLQPVDSLNNKSVIAVAAFFGPTRLIDNM
                     IVEKIGDSFTCVY"
     gene            complement(42103..42972)
                     /locus_tag="PLJ75_01795"
     CDS             complement(42103..42972)
                     /locus_tag="PLJ75_01795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD03982.1"
                     /translation="MNYEHVPCIICNSFDTVLRFSKPWGDNEMFTLVRCKHCGLEFVN
                     PRPFYNDMPQYYKGYFDKRTDRGYSNYFSKDMQNEVSRLLALNLADLNFYEFESTLPD
                     TRYALDIGCAAGYFVDYLAKRGWKSSGIDIAPDCINYATTQLHVDAYCGDFLTFNFTS
                     TYHLITLWATIEHLHYPDRFLEKIFSLLHDDGLLYISTCRSSIWSPRILFGKQWRYYN
                     FPHHLYFFSYLSLAKLLQLKGFTITRFATYGSGIGKAGSMLRTVADGLAKKLYLGDMM
                     LIAAKKQKHTFNC"
     gene            43126..44220
                     /locus_tag="PLJ75_01800"
     CDS             43126..44220
                     /locus_tag="PLJ75_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03983.1"
                     /translation="MLLYIFFTSAHFINTIRAMKISNKIIMITQNSNLFNTVKDFCTR
                     NHFDIETATELHSLDFIINEMEKTKSTAFLRAALHAFIRNEGIPFFIVTDYPVVATSQ
                     HDVNIQKIFKTMLISFMIIAQGSGLTNIKGNFFINVTGGDIKALKDTIIRPEKLIALI
                     KVNDQKVNAIMHHYADQKAFHSLFYIKPVVYKTNEEMLHELSTYVEAIHKRHALIEKL
                     IEKQEQVQGKNKDAAKVLVKINKDKIAVDREVVITRDKTYQKYEENHIYVIGDWSNFN
                     SRKVAGKVITAIKDGFADWKPGIEDQVCLHLEEAVVDHTTAATLAQIIFNDLKAYSNI
                     KIYCHEPNYKILESADGFSMIRKLVFIQKA"
     gene            complement(44217..>47639)
                     /gene="dnaE"
                     /locus_tag="PLJ75_01805"
     CDS             complement(44217..>47639)
                     /gene="dnaE"
                     /locus_tag="PLJ75_01805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPD03984.1"
                     /translation="LHNHSEYSILDGAIRIQQLIQFAVDNAMPAIALTDHGNMFGTIK
                     FYEEAHKAGIKPIIGQEFYIAPESRFHKQSTRGSNDENAYHLILLAQNYEGYKNLVKL
                     SSLGYLEGFYYKPRIDWELLTQYSTGLIASTACIAGEIPSLILDGKVSQAYKRASEFA
                     DLFGKDKFFLELQDHNLNEQNKVNKELITMAAQLNVGLIATNDAHYLNKEDAYYHDVL
                     LCIQTGKSLNDQKRMRFPNAEFYLKTPQQMETIFEDYPDALYNTVKIAEMIDCQIPLG
                     NAILPNFEVPEGYTRDSYLTELVNTGAQKRFGAIVPDHVKSRIEYELSVIQRMQFSGY
                     FLVVWDFIQFAKKHGISVGPGRGSAAGSMVSYCLEITELDPMKYNLLFERFLNPERNE
                     MPDMDIDFCALRRDEVIEYVKQKYGEDHVSQIITFNKMKSKGAIRDVARALEIPLQQV
                     NALTKLIRHEDLEEALQASDELKQLYKTNDTGKQLIDIAKKVEGLTRSAGKHAAGVVI
                     SREPLMEHVPLYKDPKDGSISSQYEKESLEKAGLVKMDFLGLANLTIIDTCIKLIKKH
                     KNIAIDIAAIPLDDAKTFKLLQKADTMGVFQLESSGMQNILLKLGPTNFDDIIAIVAL
                     YRPGPLDSGMVEEFIERKRNPKKVEYLHPLLEPILKDTMGVIVYQEQVMLISQAMGGF
                     TLPDADKLRKAMSKKKMDIIDAMEDKFVQGAKQKKIDESVARTMYELMRTFGRYGFNK
                     SHSAAYALVSYQTAYLKAHYPLEFMTALLTAQVDNIDNITKYYTDCKAKGIAVLPPDI
                     NHSYRDFSIEGKAIRYGLISIKGVGDKAIESIIEAREKVDSFHDLTQFFTHIDLMTVN
                     KGVIESLIKAGAFDPVYPNRGELFASVDVMLEKARNMQQDISTGQGDLFGASDKQFSR
                     INIEPAPAADWTENVKLQYEKEVLGIYVSAHPLSRYAKDIEKFACTPIKDLENNVNGS
                     PITLVGIVNNVQIKQSQKGNKFAQAAIEDLTGSLQVLLMPQTLSEYETLITSQEPVVI
                     TGTVEIENEKPSRMIAQKIFPFNQFKLTQISELHIIINPIGTNTELLEKIKKLLQRFQ
                     GNKPVYFHVRLPNGNENIIKANPLYHIKPDKKLMDGLTAIVGKDGITYSIGC"
     assembly_gap    47640..47739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48200..48361
                     /locus_tag="PLJ75_01810"
     CDS             48200..48361
                     /locus_tag="PLJ75_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005959792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPD03985.1"
                     /translation="MIDTKLLDILACPACKGDVEYDARNETIVCTECKKKYPVRDGIP
                     VMLVEEAEE"
     gene            48390..49520
                     /locus_tag="PLJ75_01815"
     CDS             48390..49520
                     /locus_tag="PLJ75_01815"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPD03986.1"
                     /translation="MFDAKHPVIKVAGYIIVGFFVLIIIISFGMPDFLSRMGMNPNTV
                     AKVNGEVIGYMDFIRYRDTHMFGKTDDQKQQRMIIERMIQEKLLVQLAKKEGIVVTKE
                     EIKSVIRNRFSDNAGSFNETFLKNFLEHFKIGVSDYYKYVEDEVYAGKLQNLLLAGIS
                     VSPWEIVTDYRIQNSKIKIQFAFLSNAELAKRYANDIAVTDEEVDAELKKNPKELKDP
                     KTDRQRIKEKLANAKLESIKQQLSKNINDLALAGKSFAEAQMVLKGVVSYSNDFKPGD
                     LVRERDDKGRILYPIQESGVFQSDLFTLRQGATSRLIAGFDGLYIFTPVVRVIPGMQV
                     PEKDKQSLEQNLFYAKANALYISLLTRLFEKSKIIRNQKFEM"
     gene            49576..50040
                     /locus_tag="PLJ75_01820"
     CDS             49576..50040
                     /locus_tag="PLJ75_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPD03987.1"
                     /translation="MNINAEYVNPFLEAASAVFKQLLNVDLRRGKLVIKDVPQPSHDV
                     AIIIGITGAVTGQVVFSMSLEMVGKIAKVLTPGLSDKDVANEWKDIVGEVANMITGNA
                     MNLFAYSGKRVEMTTPTVIEGESFTITLIKQTTLGINLYSPFGQLEMNVALK"
     gene            complement(50044..50859)
                     /locus_tag="PLJ75_01825"
     CDS             complement(50044..50859)
                     /locus_tag="PLJ75_01825"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD03988.1"
                     /translation="MNAVQLFLLCSVLLLQQNQPVNKEAILETILSETKPAKTIPMQF
                     NATANNESQQKGGSTPDEVLLKQGVELYNAQLYERSLQLLERLQKDYPQSPFKDQASL
                     WKAKILMQQQKYTNAMNELLAINDQSGQYPAALFMSGRVNQAMGKPFDAIEQYTKVAS
                     LYPDQDFSDDALILSAQLYISQKKGNAALQALIRVIKQYPDRETVDDAYYWLGKILAT
                     DPNLKDIELARKVYTIFLKRAEDPAYSAFYSSPLKERVADEIKRIDAIYFPEQ"
     gene            complement(50856..51485)
                     /locus_tag="PLJ75_01830"
     CDS             complement(50856..51485)
                     /locus_tag="PLJ75_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03989.1"
                     /translation="MKTIASILQKIVHTTNRMIDSIDIRTQQSIKEGFFFISFLLFIG
                     AILWGYHLGKESARPAGKPYAEITNDVFDLDIKMKREPGSFQSMLESALMEERQQSTD
                     TKIAYPTKQDEALTMPATPVDIQKDVKEVQKPLIPQNDAKPIEPYTSSDYEAVVKPID
                     TIQNVKKTSPQPSIKPTSPSAKTDEKEVIPSNNKKELKSMDIREKVFEK"
     gene            complement(51504..52397)
                     /locus_tag="PLJ75_01835"
     CDS             complement(51504..52397)
                     /locus_tag="PLJ75_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03990.1"
                     /translation="MLKRIIKVSIMGPNYRCYCIIICAIALSIAQCSLLNSASNKVVQ
                     LYHNVEINWTDQLITAKASSMPSITDDGTPVDRNTLETISINYARSRSIDEARDSAIE
                     SLINALGIIKIDPYRTLGDCIAGDDYTQKQLGLLTTDIKVRLKPDGTLATQCIATFPM
                     GRLLKLIPEALPSRDIPALPIKLPATDYTGLIIDTRGLKHTPMIFPSIFSEDGTEIFG
                     KDFIDSRDAYRYGTVAYCYTDDTALRHKKAGKLPYYCAAVRLYNACPVIANPDLRKII
                     ASNNTRNNLKKGNIIIIIDRE"
     gene            complement(52442..54115)
                     /locus_tag="PLJ75_01840"
     CDS             complement(52442..54115)
                     /locus_tag="PLJ75_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPD03991.1"
                     /translation="MLQYQITTSKSGNKSLQLVDGNKVIRLHSAYDPLKEAMRAAEEF
                     NPGRAGIIVVCGLALGYHIIALKQRFPDHSILVIEKDNELVSLVKKENPLVLSLCSIV
                     HNEEEIAAIIETWDIRSFRGTALFIHRPSYMLKRDFYDSMVAILHKQISSRISDLLTR
                     FEFEALWIKNILLNSVQMHTAYPVQSLFGKFKGIPGIIVSAGPSLIHSLEALRQAYDK
                     ALIVCVDTAYKVLQRHGIQPHIVMTLDAQTHSIKHFLGSSTKPLLLADVVSCPKVVRL
                     FNKKMFSTTAKYYNTPNGKIEKESTPIMDWVQHYTGYIGDVQSGGSVATSAFDLLLNA
                     GCSPIVLAGQDLAYTGREIHCRGTHHNDDWLSLTNRYNNLDTINQNIIRRRKIKYVPS
                     NDGTTVITDFVLDLYRSWFEDSAKKVGIPVYNTGAGAVIANTQYATLQSLVLQWQKAF
                     PSPEDIIYKQLSNKKYIDTSKLMKQLAKVKDALHNLDRAVQAGNTSCIYSILDDDAID
                     TLISPFMRKTLFYIARHNLEQEKIDTLIKDAALSAAHLLLKLFDRIAIK"
     gene            complement(54124..55599)
                     /locus_tag="PLJ75_01845"
     CDS             complement(54124..55599)
                     /locus_tag="PLJ75_01845"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPD03992.1"
                     /translation="MIPVNIPQLIDHFDKNEPVSISFYYPNEAISRIINALLATILSK
                     NDQIYLLDMMITILREIIVNATKANAKRIYFKKLNLDINDPQSYKTGIEHFKKDIVGQ
                     LDMLENELKNSNLKVTLSIRKEDKGVTFIIANPAPIHSEELNRIKTRIEKAKEYLDFT
                     QAYEDVYDDTEGAGLGIVLVILLLKNAGIDPSNYNIGTDGKVTRVVLSLPLQLRPEAI
                     TTKIKQQIINAIHDIPTFPDHILVLLQLCRNKEVEIKDITMTLMRDPALSADVLKLAN
                     SAGFVPGKRVNNLSEAVMTIGLKNLESLLITISARKILDDRFQKFEQIWEHCNRVASY
                     ARTIATKYKMNTIIEQVSLCGLLHDLGKIVLLSVDLDVTQWIAKLVSDRRIRTSTVME
                     EISIGISHSGIGELIASKWQLPEYLIEAIRHHHAPLSADSRFKGTVFVTYLANLLCGV
                     ESRRYFYYYCDNEVSRYFTLQSKSDFDNLNKLCQDIYNKLK"
     gene            complement(55785..>56014)
                     /locus_tag="PLJ75_01850"
     CDS             complement(55785..>56014)
                     /locus_tag="PLJ75_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD03993.1"
                     /translation="NKNCTYAAMLAKQFALQDIAVGDYALYAIKKITTIASVDAIPIY
                     FTIPHNKTMMDRDALIQLYYATKNHTMKGEK"
     assembly_gap    56015..56114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<56115..>57482)
                     /locus_tag="PLJ75_01855"
     CDS             complement(<56115..>57482)
                     /locus_tag="PLJ75_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPD03994.1"
                     /translation="ATIWCTGINGQYKAYCHFDHVHTIHNNDVIVFNATAYPTRNENN
                     SKQYLAQRGISSVFYLSNNSIIMHTVAAKNYRTKVQELLQHSLYRTMTHDAASLLAAL
                     YLGNKEFIHPHVQMAFKEAGVLHVLAASGLHVGIIMATILALCRLCKAPKKISVMIAS
                     SGVLIYLYISYQPVSLLRASIMCAVAATAVLLNMQKHSINLLCCAACIILCIYPYEIF
                     SAGFQLSFMATAGILLVYKQYHNGLKHCGRITSALAITFAAQLFTMPVSVYHFHELSV
                     TAFLANLVIIPMISIFMIAGLSIPLFCYAGLANVASFLIHHMYTIIVKLTQLFASLHG
                     TIYIDSLLLAGIYLIIVIIPLVYKKKANIVILCGIIALLLYHNQPQNKVIHYTGGDSS
                     WYTVLLDKGSACCIMDLKSFNDYGNLTSQLKKHAVTKVHIMLPNASNKNCTYAAMLAK
                     QFALQ"
BASE COUNT        17071 a        10719 c        12063 g        17429 t
ORIGIN      
        1 ggttgagcaa tgtcagatgg ttgatgctga tacaggtggt tgtttcaatg gtcattggtt
       61 cctccttcca gagtggtgta tatagtaccg ataactatcg tacactcatt attttgtaaa
      121 gacattttta aaagcaagat acttacaata aatattgata gtttgataaa ttttctattg
      181 atttaaatac ctatcatgtt ataaaaaggt ataaaaaata aatatcatta gtggcaggta
      241 atggatatgg ctaaaaataa tgaacacata ctggcaattg atgcaggaac acagtcgatc
      301 agggctattg tttttaatct gcagggaaat attgttgata tggtaaaaac gcccattgaa
      361 ccatatttta gcaattatcc gggatgggca gagcaggaac ctgaatatta ctggaaaaca
      421 ttatgccaaa cctgtaaaaa gctacttgcc agaggaaagg ttaaaaaaga cgcgctacgg
      481 ggggtaacgc ttaccacgca gcgtgatacc atgataaatc tggataaaaa tgggaaaccc
      541 ttgcgtccag caattctttg gcttgatcag cgacgtgcag ataaatctat tgtgaaattg
      601 ccattctata tgaaagcagg tttgcgttta gtcaatatgc ttgatgcagt tgaatatttt
      661 attactgaat ctgaagcaaa ctggattagg cagtatcaac cagaaatatg ggaaaaaaca
      721 tataaatttg tttttctttc aggatttttt acttaccggt taaccgatga ctatgttgat
      781 tcaacaggtt gccaggttgg atttgtacca tttgactata aaaagcagag ctgggcgcca
      841 ccggctgatt ttaagtggcg actgttccct atgaataagg ataatctggt gcggttggta
      901 aaaccatctg aagtattggg ttatatcacc aaaaaagctg ctaatgcaac aggcatccca
      961 caggggttgc cgctcattgc aagcgctgct gacaaagcct gtgaagtatt gggctcggga
     1021 tgcttaacac caaatatagc ctgtttaagt tatggcacta ctgctactat cgaaactacc
     1081 aatacccgat atgtcgaaat cataccattt tttccaccat atccaagtgc tgttcccggt
     1141 gcatataata ctgaggttat catctacagg ggttactgga tgttgagctg gtttgttaaa
     1201 gagtttgggg ctcgtgagga gatgcaggca aagaagatgg gcataaaacc tgaggctctg
     1261 tttgatagac tggtaaaaga aattcctcct ggttcaatgg ggcttacatt acaaccatac
     1321 tggtcaccgg gtattaagat accagggcaa gaagcaaaag gtgccattat aggatttggc
     1381 gatgtacata cacgagtaca tatttaccgg gctatccttg aaggattagc ctacgcactg
     1441 cgggagggtg cagagcgcac acagaagcgt aacaaagtac ccattgaaaa ggtgcgtgtg
     1501 tcgggtggtg gtgcacaaag tgatgtagct atgcaactga ctgctgatat atttaatctt
     1561 ccagcagaac ggcctcatac ctttgagacc tcggcattag gtgctgctat tgatgcagca
     1621 gttgggctta aactatatcc cgattttaaa actgctgtag ctgcaatgac acgtgttggc
     1681 aaggtgttct accccaatcc aaaagacgtg gcaatgtatg atgcgcttta taaaaatgta
     1741 tatcaaaaat tgtatatacg attgcgccca ttatatgagg aaattagaga aataaccggt
     1801 tatcctgaaa aaaggtaata tttttaaaga tgggtttgca gttacccttc ctttacagga
     1861 taaacccggg atagaagtga atatagcaat ttgcatcctg tggatttttt tgataaaggt
     1921 gacattatgg agatagtatc cggaatactg tgtgaagtat gtcatgaaaa tgaggcggtc
     1981 gttcgctgtg atggatgcgg gaaagccttg tgtaaaaaat gccgcttatt tgacatatgg
     2041 tgttatggat gcggacacgg tgatacaaag gcgttttgta aacagtgcaa tgaaaatcct
     2101 gccattaata tctggaaaac acccgtgtaa aagtttacgg tattgttgta actgaagcaa
     2161 agcttcgctt tattcctttt ttaaatttta tttttgggta accaaaaatt acaaaaagac
     2221 cggttttgct tttatatgta ataccatact tttctctaaa agcttttgct ttgcttccat
     2281 tttgtagaaa tggatgaatc ccgccaatca tacaggttcc aagccccaac gattctgcag
     2341 caagcattgc gtatgttgca gcaataatag gatcggcata gtcacaatag ggagagccat
     2401 aaaaatatat cgttgctggt gcatcatagg taacatagtt tatgccatgc ttcatattat
     2461 caacatagat atttacaagg ggttttacaa agtctttaaa aaacaggtaa ttagttttac
     2521 ttaaaaaaat tttcattata aataaaaacc agtcggaaac catccaattc atttgttcaa
     2581 gcaggctgca gaaatctttg gtaaatgtta aaacagattc ctttgattcc catacgatga
     2641 tattcacttc ggatggaggt atgcccatgg gtgcggtctg tgctgcttta attattttgt
     2701 ttatactgtc tttgcttacc ggggtgtctt tgaataagcg tgtgcttctt cgcttctgca
     2761 ttagcgatag taactgtgca taggttgcag cattggtagt gtatgaaagc gggaagacat
     2821 tgctggcatc tatgtccctg ccgtgaatag caatagcacc tttggggcat attgccatac
     2881 agtgaccgca tccaatacat ccaaagtaag gatttaattg gaatgcagct tttttatcct
     2941 taataaaaat atttttttcc ggacatgccg ttacacataa ctcacagcca gtgcaacttt
     3001 tttcatcaat gcctgccgtt gtgattgcat taccacgaga tgtgggtata gccatattat
     3061 tgtcctgcta catgttgtca gcaacagcct tcccaaattc agaacattta agaagcgttg
     3121 ctccttccat ctgccgttcc aggtcgtatg ttacgcgctt ttgctgtatg gttgtttcaa
     3181 gccctttgat gatcaaatca gcagcttccg tccagcccat gaagcgcagc atcatttcac
     3241 ctgaaaggat aagtgaaccg ggatttacct tatcctgatt ggcatatttt ggcgccgtac
     3301 catgtgtggc ttcaaaaatt gctgcatcat caccaatgtt agcacccggt gccataccaa
     3361 gcccaccaac ctgagcagcc agcgcatccg agatataatc accattgaga ttgggtgtca
     3421 caataacatc aaactcgtca ggacgcagca gcaccatctg gaacagtgcg tctgcaatgc
     3481 ggtctttgat gacaattttt cccgccggct gtttgccctg atacgttgac cagagttcat
     3541 cttcagtaat gatgtaatca gcaaattcct cctgtgcggt ttgatatgcc caatcacgaa
     3601 atgccccttc ggtaaatttc atgatattgc ccttgtgcat aatggttacg cttttgcgct
     3661 tgttttcaat agcatagcgt agtgcacttt taacaatatt tttagtattg gacatgctta
     3721 ttggttttac gcctattccc gtatcatcac tcaggtgctg tcccatctcg ttgtttaaaa
     3781 aggctatcac cttttttgcc tctggcgttc cctgcttcca ttctatacca gcatacaggt
     3841 cttccatatt ttccctgaag ataatcatat tgacacgttc aggttttttc agcgggctgg
     3901 gtacgccggt aagatacctc acgggacgta cacaggcata gagcttcaat acctgacgca
     3961 gggtaacatt gatacttctg atgccaccgc caaccggtgt ggtgagcgga ccttttattg
     4021 ccaccttata ctgtctaatt ttttcaagtg tttcatcggg cagccagctg ccggtgctgg
     4081 tgttagcttt ttcaccggcc agtatttcct gccagtgtat tctccttttc ccgttatatg
     4141 ccttgttcac ggctgcatcc agcacaatct gtgtggcttt ccaaatatcg ggaccggtgc
     4201 catcgccttc aatgaataac acaacaggat tatcgggtac ttcaataatg ccgttttcat
     4261 atttgattgt ttccatagcc tctctcgtat gcaatatgat ttaattatca gtaactatcg
     4321 cacataaaaa tttttttggc gatagctcct ggtatcaact cgttattaaa gattactgct
     4381 ttatgcagaa atagtggttg tgttaaataa agaaagtata gcacgaataa agtatttgac
     4441 attagttttt ggaacaaaac agaatgtcac aataaaaaaa tggatgcgat cattttttta
     4501 gacacagtat tttagaaaaa gcttaaatat tgtgattcaa tgataggaga tacagcttat
     4561 gtttttgtca tcaacatgca cggcgatcaa tagtgtttcc cctgctcgta caaagggaag
     4621 gggcaggtgg ggataatgct atgtatggat gagaggtata tttcatgaaa gaactcatta
     4681 ttattggtgc cggacctgca ggattggctg catccatata tgcggtgagg tcgggacttg
     4741 atacgctggt gcttgaaaaa atttcacctg gcgggcaggt tatgcttacc catgcgattg
     4801 aaaattatcc gggatttatt gagccagttg aaggatggca gcttgcctac aacatggagc
     4861 agcaggcacg tcggcttggc gtagccatag aaagcaaaga tgttgtgtca gtagaaaaat
     4921 atgatgacat gtttcatgta aaaacaacag atggtgctgt tattgagggg cgggctatta
     4981 ttgcagcttc aggtgcttcg ctcaagcgtt taggagttcc cggtgaaagt gaatttaccg
     5041 gcaggggcgt atcgtactgt gctacctgtg atgcggcatt ttttaaagat agagtaacag
     5101 cagtagttgg tggtgggaat acagcgcttg aggaagcact gtatttaaca cggtttgcaa
     5161 gcaaggtata tatcatccat cgtcgtgatg aattccgggg tcagaagatt ttacaggaca
     5221 gggtgctatc caatcccaaa attgagccaa tttttgatac actggtaacg caggtacatg
     5281 gtgatgaaaa agtgtacagc ataacgctgc agaataaaaa gacaggtgaa agccgtgaac
     5341 ttgcaataca gggactgttt atctttatag ggaatacttc caatacaaca tatcttcctg
     5401 aacaggtgct gaatgattca ggcgaagtaa aggttgatat gaagatgcaa acacccatca
     5461 gcggactttt tgcagcagga gatttgcgtg aaaattcttt acggcaggta ctcatggctg
     5521 ctgctgatgg cgcaacggct gctattagtg cttatgaata tataacgcat aaggattggt
     5581 gacggtttgt cgttgttata tgcagtatta cattgtacac aattatatat aacgagcaaa
     5641 cttactaaaa caatcgtgca gacgctcctg aatatgttgt atgcggttgc tgtcaacgga
     5701 taagctgtgc ggatcaaaat atttttcatt taaaaaaagt gcctttgata aggctacccg
     5761 tataatgggg atttgatgtt tggcatatcg ctgcatgata taccgttgtg ctggctttgc
     5821 aataacaaaa ggtgctgcta cggtacagtc ttcactgcca aaagccttct ggaaaaaaga
     5881 caacagtttc aaagctgtat tttcttcgca ggttataata tcggaaccat tggtagtgcc
     5941 atgttctatg gcaatgattg gacgtggtat tcccgcatcg cgagcaacaa ttggagcaac
     6001 aggcatggtg gtgtgacata caatgataca gcgagtagat ttaactgtat gctctatgct
     6061 attgtgaaat gggaaccagt accgtttcat catggcagct attgcaatct catcaggaaa
     6121 aactccgctg gtaaacatta attttttttg tgatgtatgc attttcataa ccccattgct
     6181 tgagcgtgga ggcaatgtgt aagggtccct gtctacatca ataaatagcc tgtgaatgga
     6241 ggtatgtatc accgtgccaa tttgttgtag atcaaaaagg tcgttggcac aggtatctgc
     6301 ctcaaagaac aattcaaatt cgttgagcac acaaagctgt gccaattctt caggaatctg
     6361 ataaccacca tgaggtatta taaagcagta tggtttttcc attagtttta tggtgttata
     6421 ttggtatagt agtaaaatta tattgactaa ttaagtataa caccattgaa ttgtgaagaa
     6481 cttttttatt atcgcaataa tttattgaga gaatatacaa cacttataaa aaaaggagaa
     6541 tgtcatgcgt ccctataaaa tagatacatt aggcgaggat attttagaaa tactttcaca
     6601 tgatgccaaa gccaccgttg aagatatagc acgccagctt gatgtaagta ttgataaagt
     6661 aaaaaaaagt atcagcaggc tggaaaagga tcgcatcata ttgcggtata aaactcatat
     6721 aaactggcag cgggtaaagc atgatgaggt gcgtgccctc attgaagtta aagtgacgcc
     6781 tcagagaggt gttggttttg actccattgc agaggttatc tatcgttttc ccgaagtaac
     6841 ctcagtgtat ctgttatcag gcggttatga tcttctggtg caggtggaag gaccatcgct
     6901 tcgtgaagta gcaatgtttg tagcagagaa gcttgcaaca atagaccatg tgcaatctac
     6961 tgttactcat tttatgttga aaaaatataa agaagatggt gatatactgg tagagcaacc
     7021 cgagggcaaa cgtttaccgg taagttttta gtttgcaatg ttttccctta atacggtgta
     7081 tcaaaaactt tttgcctgtt atggaccgca gcattggtgg cccggtgaaa ctccatttga
     7141 aataactatc ggcgccatac ttgcacaaaa tgtttcgtgg ttaaatgctg caaaggcaat
     7201 agccagctta aaagcacaca acctgctaac accgcacaaa ctttttaaag aagatagtgt
     7261 ggcgatagct catcatatta agccttcagg ctattacaat caaaaggctt ttgccataca
     7321 atcattttta cagtggtttc accgttatga ttttagcttt gatgcattaa atgctatacc
     7381 aacagaacta ctcagaaaag aactgctgtc aataaagcgt ataggttatg aaacagccga
     7441 ttcaatactt ttatatgcat tgcaccgaaa agtatttgtt gttgatgcat atacaaaacg
     7501 gatatttacc cgcgctggtt ttttaaccgg caacgaagat tatatggcaa tacaggctat
     7561 gtttcatgag cattttactg gcaacactta cgattataat gagtttcatg ccttgatagt
     7621 aaaacacgga aaggatagat gtacaaagaa gccagcgtgc aatgagtgtt gtattgcaga
     7681 tgaatgcaaa gaatatgatg tggctaaata aataataagt gattattgtg acaattcaat
     7741 atgatgcata aataaaattt ataatgactt tcgtacaatt gccaaaataa aattatacca
     7801 ctatcatcac cactaaacag cctcttgatg tttcggatat ctgtcagtgt agcgataaaa
     7861 atattttttt gttgtgattg tattgtcatg taaatttttt tttagaaaat aatattgaca
     7921 acaccatcat tctgatggta tcttttaagc aataaaatat aataggaggt ttgctatgaa
     7981 taccaatgca cccaaatcag ctacatggtg gattgctgtt attatcggcg tattaggtat
     8041 tgttgggaag tttgttcata tatcggtgct ggaagcctat tcatggtggc ttgtatgtat
     8101 tgggtttatt gttttagcac tggcaactgc tataaaggga ttatagcatc aacagtaact
     8161 atcgcccatg cagtagtgta ttattagctt ttatggtaaa ctaattaccg agcacagcat
     8221 acctgcagca tttgatgctg tatgggggtt gtacaggtca agctgccatg tgccatcgac
     8281 aacaaagtta taacggcata caccttcagg caagcgtttt tgtaagcgcc agatgttgtt
     8341 tttgtctttt tttagcaggt cagcttccgt gttccagtta ttgaaatcgc cagcaagcgc
     8401 aactatcgtt gcatgggggg catataccct gaactcaaca aggcgtgtac tttcattcac
     8461 tatgtggaag ctgatttgtt tgctttcagg cagtttatcc tgacgcacaa tggaatagta
     8521 tgagcctgta ttgtcatcat caatatccat gtttaccggg tcattgatcc agatgccatc
     8581 aaccataagc ttgtaacgaa ttatgcgctc ttcatcatca aatggtatta tataaaacca
     8641 cacaccatta ttgctgcgct ccattggttg ccttttccag tatgaaaaat cacctgcaat
     8701 gtataccttg cgggcatttc tgtttttata ggtaataaga gtgcctctta ttatggcagg
     8761 catgcctggc ttgctatgac tgacagttat tggggcaggt gcagatgctc ttgccaatgt
     8821 ataaagcgag tagtaaggaa ttgtttttat aggatagccg ccaggattgg aaaaaaaaca
     8881 aagggatgct atgagtgcaa tccaccgcaa tgatttttgt atcacaccag acctcaggct
     8941 cattgaaaat tttacgctca tgaattactt attaatatgt cgataggtat agagaaaata
     9001 tttatatgat tttgatgaaa ataaatatta atatataatt agaaaaatgt atttgatttt
     9061 atgtgcagag tatgaattat atgtttatgt cacattatat actgtataat gagagcccgt
     9121 tatggcggta agtgaagaga taaagaatct aacccctgaa gaagaagccg aagtaaaaca
     9181 gattgttgat gcatttgaca gcgagttgtc ttccattgcc cagatgcgag ggaagattgg
     9241 cattttgcat gctgattcaa ccgatgctga ttttaccatt acccccggta tgcgctcaaa
     9301 agaaatggaa acaacatctg caccggtaga aggctttgaa gagacggagg atattgtaga
     9361 tataaccgat atgattgaag aggttccgga agatgtagtg ctggcatctg aagctgtatt
     9421 gccagaggtt gaggaaacca tagaatccgt ggaagagttg ccttcagagg ctgtggagat
     9481 agctcccgaa gaagtattgc cgccggagtt taaagaagaa ttccctgccg aagaagtccc
     9541 tgaaaggaag cccctaaaaa aagaagcaat cagcccttta gatgaacttg aagaacttac
     9601 tcaatttgaa ccggaaacag ttgaagcgca tgatatacgt accgatgaat ttgtgaccga
     9661 agagccccgc attcatggag atgttcatct tgatgaagat atacacctat cagagctgga
     9721 aactgaagaa atatccgaaa tatctgaaat aaaggctgaa gagctaccgc aggttgatat
     9781 atcaacaatg ggaatggaag aagaaatgcc agaaccaaca gcatttgaag aagaaataaa
     9841 agaggaacct gcacgtgctc gttttgaaac tccttcattt gatgagcttg atacttttga
     9901 agagccttta aagaaatccg aaaaaattga gagcgttatt gatgaattta aaccagaatt
     9961 tgaaggcttg agtgttgagg aagaagagcc agccccgatt gaagaaggtg tagaaatttc
    10021 agatgctgag ttacgacgat taaaaaaagc tttgcttttg ctccatcctg ctttaagaaa
    10081 gaagatcaaa gagataattt taagggatga actgtcgcca caggatacac gtaaactcat
    10141 tgatcttatt atatcaggga aacctgaaga gaatatacag cgttttattg aaaagcggct
    10201 caacattact cttgaacttg aggaagaagt tccctcccac cggagggtta ttactgctcg
    10261 ccccgagtat acccgtgagg gtatggagcg tcagaaacgg ctgctgcgtc aaacgaaaac
    10321 gttagcactg gctgttgtgg taggtttcac attaacagta actgtctacc agtatattta
    10381 taaaccattt atggccagac ggcttattag taaaggggtg gcacttattc ttgagccagg
    10441 tgatccagca acaaaaaagg tgaaggatta taaaaaggca gaacaacttt ttgaagatgt
    10501 tgatgcgcac tataaaaaag attatctgta cggctataat gcttatgcac gtgcctattt
    10561 taaaaaacgt gagtttgatt atgcttatgt aaaattaaaa cgagcgtata gcataaacaa
    10621 tcgtgatgtt gaaacactca ataatacagg atattttttt gctaaaattc cggaacatta
    10681 ttataaccgc attcagaatg atgccattaa gacatttgca ccaaaagata cttatgtaat
    10741 atcacaggtt gatttagcta ttgcgctgta caataaagta ttagcgcttg atccaaacaa
    10801 tatcactgct atgtttggta ttgggaatgc ttacctgtat cagggacaat atttaaaatc
    10861 gcgccagtat tttgaaaata ttataaaggt taatcctgat tcatggattg gctatgcagg
    10921 acttcttaat cttttcattg aaagagataa tttgcccgag gtgttatcaa ttcatactga
    10981 tctccgtgca agggatattt taactgatat tccttcgccg ctgttagcaa agctggcgca
    11041 ttactactta ggtaaaaacc gctcagatac tttcaacgtt cgggttgatt tgggggtgca
    11101 gtcgcctcga ttgaaggatg aagaggataa tttaatccct gcatgccgtg gggtgcttga
    11161 tgcattaaaa gaaaaagatc ctgattatcc accattatat gtggcaagcg ctaaattagc
    11221 agcagcacaa aaaaacctta aacttacaca gatgctatta caggaagcag aagaaaaagc
    11281 acaggaaaaa ggagagcgct attttgcggc attgcatctt ttaggcgaat accactatac
    11341 catcaaacag cctgttgaag catataaata ttttaatgct gcacttactg cgtatcaata
    11401 tccacctgag tttacctatg aagactttta ttatgaatcg gagacaatag ggaaaacata
    11461 cgcatactta ggaaatatat tttactattt ctttgataag gttaaattta gatttggtga
    11521 tgaattagat gaagcgttat tggatgaaga tagtgataaa atggcaaatc ttgatattgc
    11581 ccgccagaag tatgaattag ccttgcagga aaattatagc tcccctgagt tgcgatataa
    11641 tttaggcaga atattctata taaatcaaaa ctatcagcag gcgcttgagc agtggattaa
    11701 tctgtatgaa gatattgtta ccaaccccca ggtgatgttg gctattggca atgcgtttta
    11761 tcatatgaat aatttagaag ccagtaaagg tgaatacctc aagatgattg cagtttttga
    11821 aagaaaggca gaatccatac gaaggccaat gcctgaaagg aatgagcata ttattatatt
    11881 ccaatcgctg gcttcggcat ataataatct tggtgcagta tatcaaatac aggggaatga
    11941 aacaaaaagc agcattgcgt actggaaagc cattgagtat gctacccgta ttgggaggga
    12001 aaatgaattt gcacgtgtaa atttggctcg tggatttaaa ccgcgtgtta ctcccattat
    12061 gccaatactg gatgaaagca ttccctacag cattgatatc tacagagaag acatgcgtaa
    12121 ataactttgt ttcaattaag cacaggtgtt gaaaagaaaa aatccgtatt tattaaaagc
    12181 aaaatatata ttaacggctg aacaaattgt taaaaatata attgatgccc atatttcttc
    12241 aaatgaagag acaatctttg gtgattggct ggaatgtgat tatcttagaa aaatttactt
    12301 gaataaagta actcattact acattatgtc tcctatatta caataaccag tgaaataaac
    12361 agtaactatc gcacataact attgtattga ataattatac atgacaatga atagcatgca
    12421 aacagttaaa gcatatcatg ccgatgccgt aatgcagtat gatgataatg ccattataga
    12481 acatattgct caacatggtt ttgcaaccga cagccgcttt cttttaaaaa ccgaaagcta
    12541 tgtcctttca tgtgcatatg caaataataa aatgctgtcg ctttcaaact cgcgcacacg
    12601 gattttacct catcagattg aaagtaccta cactgttgtc aatgcgttgc acaaacgttt
    12661 tttaattgct gatgaggttg gattggggaa aaccattgaa gccggcttga ttataaaaga
    12721 gcttttttat cgctttaact atgaacgaat ccttattgta tgccctgcgt cgctattgta
    12781 tcaatggcag tatgagttga aaagcaaatt caatgaagat tttattatcg ctgacagcag
    12841 ggtatatcgc agtgaaaagg gaaacccatg gaaattaaat aaattgctgt gttccataga
    12901 ttttatcaag aataagcatt ttgccgggaa actttcacaa acatactggg ataccgtggt
    12961 tatagatgaa gcacatcgct tacggcgtga tgcaaatagc cagacaaagt catatcaggt
    13021 agcggagata ttagcaaaaa atagcaaggc attgctgttg ctttcggcaa ccccttttag
    13081 aggtaagctg gaggagttat attatttact atcgctttta gataaaaatt tactggggcc
    13141 aattcacagc tttcaggaaa cctattgtgt gccggaagca gatcttacgt tgcttagaga
    13201 taccatttct aaagttatta taaggcgtac taaatacgag gttggcggat ttacaaaacg
    13261 gctggcaaag accatacgtt ttgaactata tcctgaagaa cgggagttgt ataacgctac
    13321 tacggaatat gtagccgacc agtttaacaa agcaatgcaa acaaataacc gcactattgg
    13381 ttttgttatg acggtattcc agaagctcct ggattcgtca tcgtatgcat tgctgtgtgc
    13441 attgcaaaag cgctatcagt atttaacaga aatatataat aaccatcacc atgcaataca
    13501 gagtatgcaa gaaatagatg agaatgtcat tgaagctgaa gatgtggagc gcattgtaca
    13561 ggtagagagt gctgaacttg aaaatgaaat gaatacactg gcaacactga taacaatggc
    13621 tcagagtatt gtccgcagca aaaagggtga aaaattatta cagcttgtac gcaagctttt
    13681 tgctgaaaag cataataaga ttcttatttt tacgcaattt cgtaccacgc aggattatct
    13741 tgcacaactt ttgagtcatt acaaagtagt gctttttcat ggttcaatga gcagggatga
    13801 gaaggaaact gcaatagaat attttaaaag tgaggcagca attcttatct gcactgaggc
    13861 agggggtgag ggacgtaatc tgcagttttg taatgtgctt atcaactatg atctgccatg
    13921 gtcaccgctg aaggttgaac agcgtattgg cagaattcat cgctttggac agagcagcaa
    13981 tgtcatcatc tataactttg caacccgaaa taccgtggca gaacgtgtcc ttgaggtgtt
    14041 acagcacaag cttaaacttt ttgaggaatc actgggtact cctgatatta ttttaggtga
    14101 tattaccgat gaacgttttt tcaataatat atttatggag ctcattgcag ggctaaaaaa
    14161 taaaaagcgt gttgcaaagg atattgaact gcacatacag gctgcaaaag ccagctatga
    14221 aaaaatggga caattagcgg taactaaaaa gattgatctc aattttgatg aatattatac
    14281 cataacatta caggaacggg atttttccaa taaaaggatt gaaaattttg taaacacgct
    14341 taaaaacatt gagcccactg cagttgattt tttgtctgtg caaaataaaa aaacacgatt
    14401 ctatggtgtg catcataatc catttacatt acaacctgtt aatgcacagg gtacctttga
    14461 tagtgcagtt gcgctggaaa atgaatcact ggagttttta gcttttggtc atcctgtggt
    14521 ggattggtgt gttgagcatt gtaaagagta tacttttggt ggattgtcgg gcataaagca
    14581 aatacaatat tcatatgatt gtagtggaat tgtattcaac tatctgctta ctattcatgc
    14641 taaggaaacg atgcagcgca ttgtacctgt ctttatcccc tataacacaa tggatgatgc
    14701 agcagatgat gcaacaatag aagacattgc cagtgaatca atgcgtcagt ttggtattgt
    14761 tagcagtgtg gttgatgatg ctgtggtaaa gtactatcaa cacaatatct atagttgcta
    14821 taatcaatcc cgcaaagtgc tcgatcaaaa aatacaccat atcatagccg ggataaaaga
    14881 atcgatgcta ctggaggttg atccttatac tgaacgggtt gaagaccatt actcaaaaga
    14941 gatagctcat gttaaacaac gtttggaatg gcaggaatgc cagcaaagat ggtatgatag
    15001 ggacatgcac agtgccataa cacgttcaag gaatacaata aaaaaccttg aagaggaaaa
    15061 actacagcaa ctgcagcagt ataaaaattt tatcactata cagtgggatg ctaaacttat
    15121 caatgcaggg cttttaatct gtaaacagca actaccgtga tatcactttt ttcagttttt
    15181 caatttcttt atgggttggg ataaattgaa tacccatgcc ggctttttta ttatcttctt
    15241 ccttaattcg tatccaccgt acagttcctt taagggtaat cttttcttca ggtgaaaggc
    15301 acagctctac atcaactgta ctgccaggat ggattggctc gggggttgat ataaagattc
    15361 caccttcact gatatcattt attgtactgt atgttacacc ttccatgctt tcaacaacag
    15421 atttacattt tttctggaca cgattatatt ttcgtttttc tgccatagta acctctgcta
    15481 ccattaaatt tatgatgaat aattgatact gtaatgataa actatataaa ataatattgc
    15541 aatatttttt tatgcaactc tctgtaaatg gagaggtatt gctttttggg gcgcattgca
    15601 atacgcctct acttgctgcg aggttatcat tgtttataat gatataatag gggtaatttt
    15661 ttgaataaca aataaaatga ttgcaaatgt tcatagcaat tattaaggtt cccttataaa
    15721 aataatacta ttcaggtgat atcgttgcat attatttatc tttctaaaaa ggtgttgttt
    15781 ccacattgta ctattacagt agaactaaag cgcagccggt ttttgtatac gttaaaacca
    15841 ggtgataagg ttattgtgta tcctgtaaaa aaaattgcgg atttcatatt aatgcagggt
    15901 gttgcaacat tagccgaaat tgttaaatca gaactatcaa acaataaata tactattacg
    15961 tttaaaggga taaaacgtgt aacaatagat aaaaggcatg gaatattcca atgcagtttt
    16021 actgaaattc atgagcgggc gatagttact aatgaggtaa cagagcagat aagaaaaaag
    16081 tcacaggaac ttattttttt aatcaatgta gatgagtcag ataaactcat tcacctttta
    16141 aattttattt ctgatgcggt acagctttca gactttattg ccaattattt tattctggat
    16201 tatgataaaa gggtgatgtt attaaataat tgtgatgttt catccagaat tgatgtaata
    16261 cttgccttgt tgaatgattt aattaaaaat gttaagcaaa aaagggaaaa ggaatcgtat
    16321 gaaaaagaaa atatatcagg atgaagtcct ctatacgtta gcgtggtcgc caatgtatca
    16381 atttgataaa tattcaataa caaaaattat ccctgagctt gccggtatag ttgcattgat
    16441 ggaaaaacgt agtgaggaaa cgtatcatta tatacttttt tatagctgct ggcgtgatgg
    16501 catgcgtttg ggtttaaaaa atttatttga tcctattttt acccgacatc ctgatcttgc
    16561 taaagcgatg gaaggaagac agctatggta taaatatact gttgtagatt caaatccttt
    16621 tgatcttcag gatattttgt ggtggttgat tcaaaactat gaaccggagt taaacgatat
    16681 acaaaattat gaagattcaa agcggtataa aaacatatat gtccgggaga tggaactgga
    16741 cagggagaca gttgttgagc gtttgccaaa atatggattc taatgtgata gggcaatatc
    16801 atccatagta tagatgtgtt taacattttg ctttagcgca ataaattcca gtatatactt
    16861 taaccgtgcg gcagcatcca gtggagatgc ggattctgac tcaatatgag ctaaaaactg
    16921 ctgcattcgc ctgacatttt gagtgcgttt ttctttatca gtttcaaata tatctttttt
    16981 aaaaagcagg taggtatcaa agattgattt tataatatta tctgcttcta taagataagc
    17041 tatataaagg ttactatcgg cattgtgaac attacgggta aaaattttaa gcaaaggcag
    17101 cacaatatca ggattaaggt gcacgttgat tagctctttt acttttgttt ctaattcagt
    17161 gtcggtcatt cgttttgttg ggtatgccag gtgtataatg taaggattgc ctcgctgtac
    17221 tttgctatag acaaattcaa gaaaacggtc aagtgagtcc ttccgttcca tatcgagttc
    17281 aacaaatgca ccttcaagta tatactgtgc aatctctgca agtggtttgc gttctcgcaa
    17341 cagtgattca atatctttaa tttgcttttc ttcatccata cattaaaacc ttttttgcac
    17401 tgtaatgtgt atagccttct ttaactggtt gatgctgttg ctattgtatt cacctacatt
    17461 gagcaccgcg cttaccagtg aatattgata aaaggtaaat ccagcaagcc caaaaaagat
    17521 aagctgaaaa ttattatcat gggtataccc tctgtatgca agtgcagcac acagcgttgt
    17581 tgatacaaat gaaagcacac ctactgtata ttttccagca taaaaatgac caaaaccagg
    17641 caccagcaat gaaccggttg ctgaaaccca gatatttttt tggggacgct tttcataatc
    17701 atgcaacagt tgtaaacatg ccatagcttc attgcggagc tcttcatcag atgtttgttt
    17761 aataaatgca tccacattat tttttgcatc atggatgttg tttaacaaaa tttgtgcata
    17821 gataaaatcc aaccgtgcag tttccctgta tttgccatcc gggtatatgg aaaagtattc
    17881 ctgataggtg cgatacgcaa aaaatggcga gccttccata aggcgcatca ctgcaagatt
    17941 gtacaatgca ctatcgccat acatggtgcc cgtgtatttt ttgtagcaat cgtacgctat
    18001 atcagttgcc cggtaatagt tgccccctaa aaaatatgcc cttgacatta tcagcatgct
    18061 ctgaggatac tccttgccat cagggaaaag aaactgatag cgcatacact cggtgatggc
    18121 atgataatat tctttgttgt tcataaaagt atcagccagc tcaagaagcg ggtgagcatc
    18181 aatctgttgt gcatgcacca taaaaataaa aaacaatatc atgcaacgtt tcactttttc
    18241 atccattgcg gtaaaaattg ttcagggttg tattccaggt acctgttaag ttctgtgcga
    18301 tagttattgt ttatggtttc ctgcgtatgg attgcgctat tatatgctga gtaaacattg
    18361 cctgcatacc ctattgcagt taatgtcccc aaagtgcagg taagagcaaa gctgccgttt
    18421 aaatatgcta tatacgttgc cgctgacaga acagcaacgg ttataaagct tatcagtccg
    18481 tctgcatggt gcctggcata gcaatgaccg cttcccggta tgatggcaga aagtgttact
    18541 gcaacatacg gattaatttt ttctttttgg gctgcattca atatagtgta tatctggtgc
    18601 atctgctgta gcctgtcgct atgttctgta taaacattca gtgcattgtc ccagtcatag
    18661 cgattgatat acaccgccaa tatattttga taatacaaca tttgtaaagg tttatcctgc
    18721 gctgatgtat cacacaaaga aagtgcctgg agcgcatcct ggtagtgttg cagcgatgta
    18781 tacacttttg ataaaagaag gtaatcgcag tattcttttt tttctttttt ttgcaagata
    18841 gcaactgctg tagcaagctg gttgcctttg caatagcaca gtgcaatggt gtaatccata
    18901 ttgtcatctg taagcatact atagcgtctg gattcggcta tacactggta ataattggcc
    18961 gtataataca gtgtggaaat taattgctgc tgcttgtgga caaaggcgtc atcaggatgt
    19021 acaaagaaga gtgtcagtaa cagcaatgcg ctcatggatg ctcagccagt gtttgtggca
    19081 cgggatcatg gtttggtatg gcgaagggat tgcagcgtat aatcctgtct cccgctaaaa
    19141 agccacccat gacaagtcca tagcgcataa ctgcctgtct gccgtattca ctgcatgttg
    19201 ggcggtagcg gcagtttggt ccatcctggg gggaaataac tacctggaac atccggataa
    19261 agaaataacc tattgctccg gctgcattgt gtatggctgg tgccgattgc ctccgttcag
    19321 tggttttttg tgccacgcta acatctgcat cccagggagg gaagtcagca aatgccgagc
    19381 tgttaatgat gagcgacagt gttataagca tgatagcaca ttgtttcata tactgtaccc
    19441 cacaatctaa tagttctaca tatagtaacg cttctgctcg gctacggtgc ttttaggaaa
    19501 tcgtccgtag tgatggccag gccatactat cgtgtcatca ggtagcgata gtatccggta
    19561 aatagattcc atgatctgtt gatatgaacc tccgggtaaa tcagtacgtc ccatgccttc
    19621 ggtaaacagg gtatcgccgg taaaaatatt tcctggcgaa tacaaacaga tgcctcccgg
    19681 cgtgtgaccg ggtgtatgca tcaccgtgag tttttcgttg ccaaaaataa tggtatcgcc
    19741 ttcttttact gtatgcagcg gctttggcga agcactcccc ctgtttatgt gtgacatgat
    19801 gcggttggta ataccggtaa gctgtcgtac atcgttttcg tggattaaaa gctctgcacc
    19861 tgttagtttc atcatggtgt tgttgcctga tgtgtgatca aaatgtccat gggtgttaat
    19921 gatatatttt acatgagcgt tgtgtttacg tattgcctgc atgattgtat caaaatcggc
    19981 tgccgggtct ataagcaccg cctcttttgt tatggggcac accagcagat aacaaaatac
    20041 atccattgga cttaccagaa gctgttctat aatcataact tttcctttaa tgaaaatagt
    20101 ttattctaat agtgcaatac tttataactg tttactttag taccatagtg agtaaccgta
    20161 atgtttaata agcagcataa tttattataa aaaagatata gaatcaggta gttactttaa
    20221 attcattcag attaaagaat gatattggat gttccataat aaaaatcaac atgatagtaa
    20281 tttttacaag aattttttta taaaaacaga ccatatggcc aatttttaat tgggtaattt
    20341 gtttaaacat tacaattagt ttttatacac cggatgagta aaaataagga aggaaatatt
    20401 tttgaggatt tttatattat ttgttaatat atgtgtatat aattatggca tttgtaaaaa
    20461 ttgtaggcat ggcattgcgt atgctgtgat gattgatact ataagctgtt ttgttgaagt
    20521 tagagaatga ataacgcttg attgaatact gatataagag ttgtcataca tatactgatt
    20581 tgtttgtact tttgcaaagg atagctaaat ataaatagac aatcaaataa aaggaattga
    20641 cttgattcca tagtattgta atctattatg catggtagta ataatttaat ttttgggaga
    20701 tacaacattg aaaggattgc caatgcttct gttttttaag attataccaa cacgtctttt
    20761 atcacgaatt tttggaattg cagcgcaaat ccctctgccg caaagtatac tcaaaaagct
    20821 tatagtctgg tatagtaaaa aattcaatgt attgcttgaa gaagcacata tcccgcctgg
    20881 tggattcaga acctttgatg aatttttcac aaggaatcta aaaaggggga tgcgtaccat
    20941 tgatcctgat atatattcgc tgatttcacc tgttgatgca atggtgagtt cctatggtga
    21001 tattgtatat gggcagattg ttcaggcaaa aggcatcaac tatgatgttg catccctcat
    21061 tcccggtgta atggaaagta catttatagg cggttcattt actacattat acctatctcc
    21121 gggtgactat caccggatac atgcaccggt cactggaaca ataacagctt atcagtatga
    21181 ccctggaagg ctatacaccg tgcaggattg gctggtgaac atgctgccag gactttttac
    21241 ggttaacgaa cgtgtaacca cctatatcca atcagcgcat ggattggtag cagtgtgcaa
    21301 ggttggtgca atgaatgttg ggaggataac gttgagttat gctccattat ataccaacag
    21361 gttttgtgct aaaccatact tctatataat ggacagacac aaacaggtaa caatacaaaa
    21421 aggtgatgaa ctggggactt ttcatcttgg ctctaccatt attgttttaa cgcaaaaacg
    21481 gtgcagattt ttacctgcga ttgcaggtaa aaaagtcacc atggggcagg caatagcgtt
    21541 tttttaagat atcgtaccaa actgttatat gtatatggca gatgctgacg gtaacaatct
    21601 gtttattttc cacttgctat tttttgttaa ttttagtata gtataggtgg tggcttgtat
    21661 agaggaggaa catctatgaa agatgttgct gtaaaagaaa gcgctatttt taaacaaatt
    21721 gaacattggc gaaggcagaa agctgtggta ggggataaag aaaaagcaaa catactgcct
    21781 tttgttacca tttcgcgcga atatggatgt ggtggttttg atctggctgt aaagctatca
    21841 gacattttaa ataatgaata taacgccatg cctatatggg cagcatatga taaacagatt
    21901 cttgatacca ttactactga tttaggattg acacaaaaat taaccgatac actaaccaat
    21961 gctgcccgaa gtaaaatgac tgagctgttc cagacaacat tcagtaaatt tcctcctcag
    22021 gtagcagtat acagaaaatt ggcagaggtt atccggacaa tggcaataaa tggacatgtg
    22081 gtcattgtag gaagggcagg taatatcatt acccgtggta ttccgtatgg tctgcatgtg
    22141 cgcattattg ctccattagc atatcgtgtt gaacgtatag ctgcaaaaaa caaagtaagc
    22201 aaggctgagg ctaaacgtat cattgagaag aaagaaaatg aacgtgagag ctttataaaa
    22261 gtttttttga aatttgatct ggaagatacc cataactatg atattgttgt caataatgaa
    22321 cgttttgcaa ttgacgaatt agcacgtctt ataattgaga ctatgaagca caaaggtata
    22381 ttagcgtaag tttgaagtta aactatgcag cagtttgatt gtaacgaaac atctattgta
    22441 tactatcccg aaaaatatat tattgtttgt attgcgtatg gtgatgggaa gaaggtatgg
    22501 gcaaaaaaaa tgcacgaacc agttgcaata cagaatgtcc tttttgatga gcgatattac
    22561 tatattgcct gcaaaaccgg tgatacagaa ggtatatttt taacattaga caaaagcaac
    22621 ggctctactg tatggttcat accgggttgt accagcctgg aggttttgta cagggggtat
    22681 ctgtatctta tctttgttga tgataatgag cagcattttt taattaaggt tgaatgtgat
    22741 gagggcaata agctgtggtt tcaccagata gatagtgacc tgtattatta tcactttaaa
    22801 gaagattgca tcgttctcca ctatgcatca ggcaaaaaag agctgatacg gtatgacggt
    22861 aaacttttat tgcaatagta ttatttacca gtaactatcg ccaaaaattg ctgtaaggat
    22921 ttctgaattt ctaaatgaaa atgaaaaatg taccgatagt tatcaataga agattttagc
    22981 atagtatgag gttttattat gaaggcaata ggaataccaa ctgaagctgc cagtatatat
    23041 aataaggctc tggcgctgtc caatatgggc aattatgatc ttgctataga acaatatatg
    23101 cgagctgtag aagtattccc tcaatttatt gaggcatata ataacattgg tgaactgtat
    23161 tcgcgaatgg gtaatagtga aaaagcaatt caggtatata gcaaggcatt gtccataaaa
    23221 aagaatcata aggttttgct taatctgggt gttgaatatt ataatgcagc tgattatacg
    23281 atagccttgc aatatttcaa cgaatcgcta ttgctgaaaa aagattttct tgaaggtaat
    23341 ttttatactg gcatgacgta ttttaatctt aaaaattatg tgtcggcaaa aaaatatttt
    23401 gaggctactg tcgccattga tgcccatcat ttaaaggcaa actatctttt agcatatata
    23461 ttgtatgagg ataaggaata tctgaaagtc attgagtgct taaagaggat agagcatatt
    23521 gcagatgatc tcatgtttat taaccggtac tatggttttt gctactatca tttaggtgat
    23581 tataaaaagg ctgtgaagtt tttaagtgct gcacttacca acagtcccaa atatgcccag
    23641 tttaaagcat atcttgaaag tcttacgtat gaaaacaagc tgaaagagat tggtgatatt
    23701 gatgctacaa tacaagcact ggaacggaag ataatggcag aaaaaccaaa tctgtcagaa
    23761 tatacacgac tcagtatgct ctatatattt aaaggtcaat ataaaaaagc agaagacctg
    23821 cttgtaggtg tgaagtcaaa aatagcaaga taataataac ccacgggtta acccgtgggt
    23881 tgtacgtgtt aacagctaca gccatcatgg gagccgccac aagcatcaca tccattgcta
    23941 tattcaggtg aatcgcttat attaatgact ttaatgtgga aatgcaatgt tttccctgcc
    24001 aatggatgat tcatatcaac aactacctcg tcatcgccga tttctttaat gacggcgggt
    24061 atttgtgaac cgttgtccat ctgcaaagca atggtcatgc caatttcagg tttaaactgc
    24121 tgttgcacct cttcaagggg gaatgcatat aatagatttt catcgtagtc accataacca
    24181 tcttcaggtg taataacgat atctttttct tcatcaagtt tcatgccaac cactcctttg
    24241 tccagaccgg ggattaccat gccggcacct atctggaatt caaaaggtgt accatcaagg
    24301 cttgaatcaa acacctgacc atcatcaaac gtgccggtgt agtgaatggc tacatattta
    24361 ccttctgtta ccataaaaac tccttaataa ttataattgg caattgctca ttgtatcaac
    24421 taacgatgct gtaatcaaat aggtaagagg aaggattata cgatgattaa agcattatac
    24481 tgttgcagta ttttacaaaa agcaatacat ccttaccaca atacaataca ctatgttgat
    24541 gtcaatatca atatttttta caacaattac atgctattga tgagaaaaaa aataaactgt
    24601 catcagttgt tatgacagct ctggaactgt tcattttttt agtttgtgca cgctcataac
    24661 agtgccctca ggcccccatg cttctgactg gctataaaag catttttcgt tgctgttcca
    24721 tataaaaaca gtagctcccc caactggctt tttattttca tcgacagtaa agtgtatcag
    24781 tagttttttt atcggcaatt ttccagtgaa tggttttaac tggattatca gcattgtcag
    24841 gattggtgat agttcctgtg ttatttgact tgaattcaca ggttgtgcat atatcataag
    24901 tgtatagtaa atctggattg tttgccgggt atcacaaaaa atcattgaca tttatgcaat
    24961 actattttat aatatgcaat aataaatgga ggtgtcatga aatctataca gataggtttt
    25021 attgggctgg gacgcattgc cgatcttcat tatctgggat ataccaataa taaacaggca
    25081 aaggtatatg cggtgtgcga tagtaacctt gaacttgcac agaaaagaaa aaaacaatgg
    25141 aatgccgaaa aagtatatac cgattaccgt gatcttgtag ctgatgcaca tgttgatgcc
    25201 gttgaaattc taacccctca ctcgctccat gaatcaatga cggtagctgc cgctcatgcc
    25261 ggaaaacata ttgcactgca aaaacccatg acggttgacc tgaaaagttc cgaccgcata
    25321 attgctgcga cagacactgc cggtaaaatt tttaaggtta ccgataacta tccattttac
    25381 ccgcctatca ggctggcaaa aaagattata gataacggcg aaattggaaa acctattgcc
    25441 ctgcgcataa aatttattgg aggaggcagc ggcgggtggc atgtgccgcc agaagcatgg
    25501 cagtggcgga ttaaggaaaa taccgagagc ggtggcatgc gcgggcttga tacatttgat
    25561 catggacatc acctatggac aacagcatgg tatttatgcg gcggtgttga aaagacagca
    25621 ggatggattg actcaattga tggcattatt gatgctcctg cagtcatgat gtggaagtat
    25681 gacaatggat gttacggctc aattgagttt gttcatttgc cttcgcttac catcccctgt
    25741 aaatattatg ccaatgatga gtggatagag gtaagtggca ctcatggtat agtagttata
    25801 cgtcgatgta cggggagtat taataaaggc ccggttgttg gagtttttac taacaaggga
    25861 tggaaaaact acaatgttcc atccgactgg agccttggat ttgtgggcgc tacccataat
    25921 tttataaacg caattcttgg taaagaagag cccatgcttt caggaaggga tgcacgtgaa
    25981 attttgcgct tttctttagc aattcaaaaa tcggcccggc ttaacaggga ggtttataca
    26041 cacgaactgg acagcaaatt tccctcactt tactatgttc gccaccactg caaagagctc
    26101 aaggcaaaaa agcccaaaaa aagcattgct gagcgccttg gcttatcggc aaactttgca
    26161 aaatatgcgc cacaggcaaa agagctgacc cggcaattta taaagcggta taatcccgag
    26221 gcagcaaaac agtggacagt aactatcgcc ctgcatataa aaaaagaggg caatgccgat
    26281 gagtttctgt acagcattgc aattaataat ggcgcgttga tggtaaagga aggggcgctc
    26341 cctgaaaaat gggacatgaa gattaccgcg caggcaggcg tatgggctgc aattttactc
    26401 aaaaagaaac gcattgaaat ggcgtatctt caggggttgc tgaaaattga agggaagtcg
    26461 gaagaggcgc tgcgattgcg ttcagcattt ggaatttgat tgaggggagg tttcgtatga
    26521 aacagagtat agcacaaacc ggtttatcac tcataacaaa attaggatat gccgttggtg
    26581 attttggcgc caacatggtg ttccagtcgg tagttctttt tttaatgttt tatttaacag
    26641 atgttttttt gatacctgcg ggagttgccg gcacaatttt tttaattgca aagatatggg
    26701 atgcagtaag cgacccaatg atggggtaca tcagcgatag aacaaaatca cggtgggggc
    26761 agaagcggcc atatcttctt tttggtgcgc tgcctctggg tatttgtatg atgctacttt
    26821 tttatactcc tgcgcttgaa ggttcatcaa aaacaatgta tgcgctggtg atgtttttgc
    26881 tggtatgcac tgcgtacacc gttgttaata ttccttacgg cgctttgacg gcaaacctta
    26941 cccttgatac caacgaacgc tcacgcctaa ccggttaccg tatgtttttt gcgatactgg
    27001 gcacattatt tgtagcaggg gcaacaaagc ctcttgttgg tttgttcgat agttccgttg
    27061 aagggtggcg ctttattgga atgttgtacg gcgccatagc agcatttttt acccttgtaa
    27121 catttgccac agtaaaggag cgcatacagc ataaggatac cgatgaatat catctgcgcg
    27181 atattgccac aacgttaaag gtaaataaac cgtttatttt tttatcgata ggtgtgtttt
    27241 tacatttgtg tgcaataggc gtgctggcat cgatggtaaa ttatctgctc aagtataatt
    27301 atgcaaaaga ggaatttgtt ccggttgcat ttttatgtat gtttgcaaca gcggcggtgg
    27361 caatacctct atgggttttt ttatcgcaaa agatgggcaa aaaacatgca ttcaacgcag
    27421 gtatggggat gcttgctgtt atactgcttg ttttgttttt tgttaaggaa tttaatagtg
    27481 ttctttttat tgtgctattc gtcattgcgg gtatcagcat ctccaccgtg ttcttcagtc
    27541 catgggcaat gatccccgat actgttgaat actcgcagtg gaaaacaggc atacgcagag
    27601 aaggcatttt gtatggattt ttttatttca gccagaaatt agcagcagcc tgtgcgggat
    27661 ttatcatcgg tattgggcta acgctgtgtg gctacttgca accgtttctt gttgataaca
    27721 cattaacctc ccgaatgcag actgaaggtg cgcttaccgg tatacggctg ctgagtacca
    27781 ttatccccat gctgcttatt atcgtcggta ttgtgtttat atggcttttc cccattgatg
    27841 agaaaatgca ccggaagata gtggaagaaa ttgggtaagg tggttatgta aaaattcatt
    27901 aaaaatcatc agaacaatta acaaattgca attaatgtag tacattgtaa acatgattta
    27961 ttatgataag gaagttcaaa aagcaattaa gaagagtgat attccgacaa agatagtaaa
    28021 acgatttcat aatgcctttg tatcgcttga tttaacaaaa gatttgaatt tgtttgatgt
    28081 caaaaaggtg aaaggaagtt ttagaagaaa ttattgtcga ttacggaaag gaaaattcag
    28141 gacaatgttc tttatagaaa atgatgactt ttatattgtt tatataggaa agcgagagga
    28201 ggtatatgat caatggcagt aacaagtgta cgatttaatg caaaagagga aaagatagta
    28261 aagattctta aaaaacatta taactgtgac gcttccacgc tcataaaaaa atccctgtgg
    28321 gaattatatg aggatataaa agataagata atagaagcct ttgaaaagtg tgaaaagggt
    28381 ggaaagacaa agtttgtaac cattgataaa atggtacaat aattataatt gtttataatt
    28441 gaaagaaaag cctcttgtgt acagtagtaa agcctggttg ctatatcata atgcttatgt
    28501 ttcctgcata gtatgccacc aaacattacc tattaaaaat acattaaaac ttctttaaaa
    28561 agtttacata tcgttgtcat gatgtaacat tgtcatatat attatttttt tgttgcgcaa
    28621 atattctatt gttatgaact tacaaatagg tatatcattt ttttctataa tgtatttatt
    28681 atacaatttt gtattattat gaaaaggata gcatgactga tggattatgt atacataccg
    28741 gttaggctaa cgatatatct tttataacag gagggtatta tatatgaatc ttaaaaaact
    28801 gatacgcaat tatttgagct ttgtcaattt cagcacaatg attcaattaa caatgcactg
    28861 gctctggttc aggcgaaaac gggtcttttt tatctttgaa ggtcgtgaat acacgtataa
    28921 cgaggtgtat aaacagtcaa agcgctatgc aacatttttt ttaagcatgc gtaaaaagct
    28981 tattgaaagt ggaaggttgc atgacaatca aaggcttgct tttggcgttt acatggataa
    29041 caacccggaa tttatctttg cctcgtttgg tgcgggtttg agcaactcta tcctctttgc
    29101 tatcaatacc ggattcagag gggaaacact ggcaaaggtt atagatcagg caaacatctc
    29161 gtttctcatt atcaatgaaa gcacagtaca tgaggttgca ccaatcatca acaatagtac
    29221 ggtgattcat gcagatgaca tctattatgc tggcaacaac aatggttacg ctgctcaggg
    29281 gttccactcc cttgaggaag ctgttgacca tgctcagcat gaaaatatcc ccaaaaacta
    29341 tcgaacaaaa attgataatt ttagtcccgt cattgtcatc tatacctccg gcacatcagg
    29401 gctgcccaaa ggcgttccct gtacccatat taaactggtg ggagcaggat ttattgtgca
    29461 atttgccgtc catctcaata aaaatgatcg cggttatatt tccatgccgt tgtttcattc
    29521 caatgcatgg tatattggaa ttttgccgca attgattgtt ggcggaagct ttgttttgaa
    29581 aaggcgtttt tctgccagcg cctttgagga ggatatgctg cgctatggag taaccttcct
    29641 taactatgtt gggcagccat tgcattacat tattgaagca ctggagcgca agtatggcag
    29701 cggtgaagcg gtggaagcag cgctggcaca tcatccgcgc aataaatttc gcaaagcgta
    29761 tggaaacggt gcaacggtga ccgatcgtat aaaattaaag cgctacctgg gcatggagca
    29821 catttacgag atttatggtt caaccgaagc ggttattacc actgccaacc ttccggggga
    29881 cccaatcgat tctgttggca gggttgattc atctatcaaa atattgaatg aagaagggaa
    29941 ggagtgcgag ccgggcatag tagatgaaaa tggagctctg attaattatg ataaagccgt
    30001 gggcgagata tgccgtaagg ttggcgatag taaccttaga tttgaaggat attttgggaa
    30061 tgaaaaggca accagtcaaa aatttcgtga tgggtggtac cattcgggag atctggggca
    30121 catacgaatc atcaatggca ggcgatattt gtttttcaat ggccgaacag atgactggat
    30181 tcgcaaggat ggcgaaaatt tttctgctga gaacgttctt gattatgcac acagaattcc
    30241 aggcgtggac attgcaattg cttacggcat ccccagcgag gtttccgatg aaaaggttat
    30301 gattaccata cagatgaaag agggcgcatc ttttaatcct gatgaagtgt accactggtt
    30361 tatgcagcag cagaaagagg gtgggatgga cccaaagtgg atgccggatt acattagaat
    30421 aattgataaa ttttctgtga ccaatacaca aaaaattatg gtgaggccct ttaaaaaaga
    30481 aaactttaat cttgataaca accccaccat gaaaatttat ttccgccagc gaggcgacaa
    30541 tacctacagg cttttaacat acgatgagtt tgttaaaatc aaagaagcat ttaagacaaa
    30601 tggcagggag gctgttttat cacgaaactg aaaaaatctg tgattatgga taaatgatgg
    30661 tgtagctttt taatgaacct gtttctggag ctggtgcagg agcaacgttt tttatttgtt
    30721 gcatgttgtt catgacaatg gtactataat tttatagtac catcatcctt gattggtaaa
    30781 ggaattggat atggaacaat tgcgcagctt tacaagccca tttgatatat cacgatgcaa
    30841 agaatgcggg gaatgcttgc accaatgtcc ggttttacag atgaaacctg atgaagctat
    30901 cagcgaaagg aaaaaattaa acagaggcag ggtttctgca aaaataatga agcggtgtac
    30961 cacctgtttt gcctgtaacc ttgtgtgtcc gcagggatgt aatcctgctc agcagataat
    31021 tgacctatgg cataatgcat accgcagtaa aggtctgccg ctgagagcaa ggtattttga
    31081 ccccaatcat agtacaaact tccgcaccta tgtcgttgac aggctgccgt atgacgagcg
    31141 agcactgatt gaaaaatgga aggatacctc tgaatgtgcg gaaatattgt atcccggctg
    31201 caatatcatt accgcccctt atttaacaca aacatcgctt ctggaagggc tggaaattag
    31261 aggaagcctt gatgtttgct gcggcgaaac ctattaccgc atggggctta tcgatgagat
    31321 gtacaaagtg gcggaacgtc tggagcgata ttttaaaaag ttaggcgtgc ggaagatgat
    31381 catcccctgt acagcagggc gcaacatgtt taccaatgtc cttccatcat ctggcgttaa
    31441 atttgatttt gaggttgtgc atatacttcc ctggctatgg gaacggatgg aaaaaggtga
    31501 gatagtaatc aaagaaaaat taaatatgac tgtgacagta caggaatcat gctacggcaa
    31561 gattttcggc aatgattata tggatattcc gcgaaagatt ctcaacagca taggtgtaca
    31621 ggttattgaa gaaaaattga tcagaagcca tgctctctgt tgtggcattg ccggaggctt
    31681 cagtgttaaa tcgggatatc atcctgttga tattgctcat gaaacgtttc gctctcttgg
    31741 gttagctaaa caaacgggtg ctgatgccat tgcagtttac tgtgcaggtt gcctccagat
    31801 gcttacggtg gggcaaattt tatatccatt caatagaatg ccggtgtatc acattctgga
    31861 acttttgcag atggcaattg gcgaaaaacc tgcacggctt aacaaaaaca gagcacaaac
    31921 attgtttgct ggcgttttaa aacatcaggt accactgctg ttctcgttaa accgtcacag
    31981 ggttgatgta aaaaagctgg atttttaagc atgaaacaat accgctggtt tcgtgacaat
    32041 cgctgccgtg tatgtggtca atgcctttct ttttgtccag tagtttcgct cccagccggt
    32101 gcagcacgca gggatagaat cattgcacgg gaaggctcaa ttaatgagtc actatcgcta
    32161 tcatattgta caacctgcaa cgcttgcaac gttatatgcc caacacaatc acatccatac
    32221 gagcttgttc tggaacgtta tgatgaagcc cagcgcaata aaggcctgcc ggggattgca
    32281 cgattggttt tccccaatga gtatctgaat atatgggcaa taacaggggc tcttttgact
    32341 cggaaagaaa aacacttttt aaaacagtgg gaaaacaatc tccatgaaaa gcatagcacc
    32401 attatgctaa ccggatttta tacaaacctt gttccttttc ttttagacca tgtaatgttt
    32461 caaaattcca ccattcccat tgcggggagt gaagggttat ggggatgtgg tggtgatgct
    32521 cataagctgg gacttgttga catcactgaa aaggtggctt tgctgttgaa aaacaagctc
    32581 cattccatga aggttaaaaa ggttatctgc tttatggaag ccgaggcagc catgcttcag
    32641 gagatacttc cccagtatta tggcattgat tataatatca ctataatccc gatagaagaa
    32701 tggcttttgc agcaaataaa acagggcgat attacaataa gtgctcccct tgccatacgg
    32761 attgccgtgc atgataattg catgtcacga tacctcaacg gcaaaccgca aaaaacaatg
    32821 cgcgagattg taaaacggtg cggcggctta tctgttgaga tggagcatgc aaaagaaaaa
    32881 tcgctgtgct gcggatgggc agccacaata ccatcgcttt accgcaatgc ctcacttttt
    32941 ggaattgccc tgtatctgct gtatagcctt gacaaaaggc tctgcgaggg tgtggatgca
    33001 aaggctgatg taatggtgac aggctgccct gcttgctaca tcttcctttc agttgcgcag
    33061 tatgtaacca gacgaaaaat aaaaataatg catcttactc aggttgtttc gttagcagca
    33121 gggcagccgc ataatgctat gatggatagg cgtgtccggg atattcttgc agggattata
    33181 ttttttttgc tgcgatggat ttcttcccgt tctgtccgtg agcatttcca tcctgcatcg
    33241 cctcaaactg atgatgtcca gacaatccct gtacttaaca aatccgatgc aaaaactctg
    33301 caaaggatac gcagcattct ggatagccct ctgttaacaa atcgtatcac gcaatggctg
    33361 attgcgatga tggtacggct ggctgtccgt ttgtatgggc tatgccttgc attcaaaaga
    33421 aaacaattca ttcgcaaaca gtgctcaaag gtgtctggat aggtaccggt aaaaaagctc
    33481 atggcggcag tgacagggat gcattgcgta aatagcagtt actgcaagga gtaaccggac
    33541 aggagttgtc cccacctgtt caccagccct gtcggtgata ttcctcctga acctgtgaac
    33601 accagctgcc aacattccag ctgccaaata cacccatgcc gccaagctcg ttttgaccat
    33661 aatataccgg aacatgtatg agcgatagtc ccctgtattg atctgctttc tgtaatgcct
    33721 gcatcaattc agaaggggtg taaccgccat agataccctg cacaccatga accgaatgag
    33781 ccatcgcaac ataatctaca gcaacactat ccgatgtttt gtattcgctg ccatactgag
    33841 catactgcaa gccggtgatg gctgccatcc ttctgttgtc aaaaataatg atggtagcgt
    33901 tggcaccgtg ctcaacacca tcaattaaaa tctgtggatt cattataaaa ctgccatcac
    33961 cgcagaaagc catcatcttt ttgtcaacaa tgccggaaac aagcaccgat gatgatgcaa
    34021 agcccatata cgatgctcca gtctcggtta tggtttgctt ctcgtgctta tcttcaataa
    34081 tctggaagcc attagcctgc acatcgccgg cgtcaaaaac ttttatataa ttgtgttcat
    34141 ccgcaaaatc gcatgctgtc tttattgctg caggctgtgt gagcacatcc cgcttccaca
    34201 tctcatcaaa tagtaccggg caatcaaagc gctgctgctt caatgcctgc cattgtgctt
    34261 tcttttcctg taatgctaaa aaccattcag agtgactatc gcccatagaa aagccgttag
    34321 cccgcaggtg ctcaatccac cgttgcaggt taccctgtgc gtcgccaaca actgaaacac
    34381 agcggttata gtgagctgcg tgaaatggat tgacattaaa gttgacgatg gaacgcgcat
    34441 tcttccatgc agtgcccgag caatcccact gacacacgcc gcgtgcacct ataacaatga
    34501 tgcagtcagc ttccattgca aagttgccgg acagtgaacc cttggaaccg cccacgccca
    34561 tgtacctggg attggtgtag ggaacgatgc cggcgcttga gggaccgctg acaatagcgg
    34621 catcaagcag gttggcaagc gtttcaatat aactgccgca accccgtgcg ccgttaccaa
    34681 tcttaatggt tacccgcttt gcattttttg ccagcgcggt tgccttttca aaaacagcat
    34741 catcgtagca cacaacagga acatggcgat gtgcatgggg aagctgcaaa agattaaagt
    34801 gtgtaagcac tgccggctgt acattcatgg gcgcaagcaa aaaaaacggt tgagcaaacg
    34861 gcagcccttt tgtgcacacc gctccgcgtt taagcgcaga gataacgctc catggctcgt
    34921 acagtgcata ggcattcccc atgacactgc acagcttaag gaacagcgcc tgctcatcct
    34981 ttggtatttg ctgcatgttg aagccttcat catgggttgt ttcatcgcca tatatgtggt
    35041 aaacgccggc accgtttgat gctgatgcca gcgaacctgc aaatgcatgt aatgcaccgg
    35101 gccctattga cgtaacaacc gccgccactt cgccggtgag catattgagc atggtagcgg
    35161 catgcgaagc tgccgtctcg tgtctgaggt tataggtgtg caccagtccg tgatattcgt
    35221 aaatacggag gacctccgca atggcggttg aaccatgtcc aaagatgcca aaatacctgg
    35281 tcacaccctg ttgtaacaat ccaagtacaa gagcttctga aacagtgatg tcctggtact
    35341 gtgcgatagt tccctgtaaa acagctttgt gcaatgagct gtgacgtgca atggcagccg
    35401 ctctttgctg tatgcctgtg ttcatgatgg tagctccttt gaaaaatagc attattctat
    35461 caatgtcatt ccgggcttga cccggaatcc agataggtaa cagagcgcct gaaaccagga
    35521 atatggttca ggatggctgt caccaaatat ttttatcccg tatgattgtc cataatccag
    35581 ccatcgtgaa ctacgatatc cttgctacga tggcatgttt aagttcatcg tcaagcgatg
    35641 gtacatccat actatcaata aagccaggat attctaaagc aggctttacc acctcatcgg
    35701 gcaatgcctg cataatgtgt gccatggaag catgcgtcag atttgaaatg gcagataaaa
    35761 gctggcggga gctttgctgt gattctttac ggtgcgctgg atagccgttt ccccattgaa
    35821 ccgaaaacaa tgcgtcaaat ataaaagtaa ggttttgctc tgatgcccac ccgtatccct
    35881 gattaagcat aagcgagata cagttgccgc cattaatctg cgcaaaaagc catgcatcaa
    35941 gcggtgttac aatatgtccg cacattacag cgggatattg cattacagcg tttaaaaatc
    36001 cctgtccggt accacaccct ccaacaacaa ggtcaacccg ttttgcgttt aacagcaaag
    36061 cggccatcaa tcccgtgtga atgtaggtaa tttccggcga ctgtccgctt atggtcatgc
    36121 ctgcattgat aatttcatgt cctctgtttt gtaacgcctg catgatatca gcatttttat
    36181 ccgcggcact tgtttcgttt ataactgcaa tcttcatgat gctcctccta tcctcaggag
    36241 tgtacgtgcc tcatcagggg tagcaatgtc tctgccaagt tcttttgcaa gccgcacaac
    36301 cctttccact aactgtgcat ttgatgttgc aagcttcccc ttatgataaa acacattgtc
    36361 ttcaagccct gttctaacat tacaacctgt aagtattgcc atggtgttca tggtaagctg
    36421 atatttgcca ataccggata cattgcaaat actgttgtct ggtagatgac gcagcatcgt
    36481 taaaaaattt tcaggcgtac cgggatatcc tcccatgcca ttgacgccca tgactaaatt
    36541 tatataatat ggtaaagcaa gtagattctt tgcaatgagg ttttcaacct caaggaacat
    36601 ggaagggttg taaacctcca gttcaggctt tatggtccgc tgtaacattg cctgcgcaaa
    36661 ggcttcaatt tcatggcgat agttatggaa agggagctgt ttgccctgat aaaaaaacac
    36721 caccgagccg caattgagcg aagccgagtc gggatgtgca tcaagcgatg caatgcgctc
    36781 ctctacagga agccccggac cgccggttgt attttgaatg ataacaggac atttgctttt
    36841 aataccgttg ttgattgtag caaaaatatc tttcgatgca gtttggttcc catccctgtc
    36901 acgcgcatgg atgtggacaa ttgccgctcc tgcgttgtag caatcgtacg cagcctgtat
    36961 aatctcgtcg ggctgttcag gtaatgccgg atttgcttcc ttgccatgaa tcccgccggt
    37021 cagggctgcg gttatgataa gcttttgcat attacacctc gtgctgaata atttttcccc
    37081 attgcattaa agcatacact gcaacaaccc agtgactgat ttcatgaaac accggtatgc
    37141 ctgcatgtat taatctattg ttcatgcgtt gtgaatacgt gccttcagtg caactgcaaa
    37201 caatgggttt tttttgtaaa tcagaatgcc ttttcatctt ttcaatgatt gcctcatcaa
    37261 gcggtgtatt ctggaaaaca aaaaacggca taatgatatc cacgccgtcg tcattcataa
    37321 gctgctgcaa tacaaagtca taatcatcgg aggtggctga accagtcaca tcaacaggat
    37381 tatgcacaat gtaaaaaaag ctgaaatgct cccgcatggt tttttttgtt gtatctgaca
    37441 gcggcgcaag cacaaggttt ttctgcggga agaggtcaag tgcattgacc attggtcccg
    37501 caccgtttga aaccagtgca acacggttgc cttttgcagg cggctgcatg gataacgcca
    37561 tacatgctgc aaagcattca tgcaggctat cggtaagtat cgcaccggca tcctgcaaca
    37621 tgtcatggta cagcgtataa cttccgccat acgccccggt gtgcgatatc gcggcctgtg
    37681 acgatatgtc gctcctgcct gttttataca ctaccaccgg tttgtgtttt tttattgcat
    37741 gagctatcgc ttcagcatat ttttttccct tttgcagacc ttcaatatag ctgccaataa
    37801 caccggtgcc ggcatcatcc atcaaatacg aaataagatc accctcatca acatcaacac
    37861 ggttgccgta acttaccatt atatgtaaac ctgtagtttg tgccagttcc ataaatgtga
    37921 ctccccatgt gccgctttga gttatgaagc tgatggttcc ctgcggcggg cgggtaaacc
    37981 tgtctctatg ataaaagaat gaatcaaacc gtgagagtga gttaaacacg ccaatgcaat
    38041 ttggcccaat aatgcgtata tcatagcatt ttgcttttgc ttcaatttct ttttccagct
    38101 tttcacgcgt gccccccagc tctttgccgc cgccggagat aatgattgca ttatggcagc
    38161 cttttgcatg catctggtca agaatttcag gtaccatctg taaatcaacc gcgatgataa
    38221 cgacgtcagg tgattttgca agctcattga ttgaatgata tgcggtaagc ccaagcacct
    38281 ctttttgatg gggattgatg ggatagatat ctcctagaaa gcccccattg ataagggtgt
    38341 caacaataac gttgcctatt ttgttgacaa ccgttgaagc gcctaccacg gcaacacttg
    38401 ctggattaaa aaaagtatgc agatgctgca cagcaggttt ttcaacaatg aaaggatcgg
    38461 ggatgggtgc atggtaaaag gtaaatgacg catcggcaac aacgcagctt ttttcatcaa
    38521 caataattgg attaaaatct atggattgta tataccgggc gccatcacaa atcaacctgt
    38581 ttaaattcat gaagcattgt atcaatggtt tgcaatctat tgttggaata tttctatacc
    38641 ccatcagaag aggatagcat cgcaatctgg aaagcatgga catcacatcc tcttcagtaa
    38701 ccggcgcatg gtgaaacacc acatccttaa acagatcagt ataaatgccg ccagaaccca
    38761 gcatgacggt aggtccaaac acagggtcat tggtatagcc ggcaatcatt tctatatggc
    38821 catgacattg ttcttcaaca agtatcccat tatagcctgc tatgtttttt tgtttcatgg
    38881 agctgtcgag catggaaaaa cattctttta attcgtcata acttttaata cctgttatga
    38941 caccaccaat ctcggttttg tgcagcaatc gtccgcttac cttgcacaca acggggaatg
    39001 taagcaaggt tgaagcttcc tttagctgtg cgatagttgt tacaaatttt ccttttggtg
    39061 tgggaatggc atagcgtgat actatgtgct ttgattctat ttcaggcagg attacgttgc
    39121 cggatgctat aagctcatta tgcagctttt taaagtcaaa accggcgtta ttcattggat
    39181 gcctcttttg cattgttagc ttcctccgca cttttgacaa tatactctga tgcgatggcg
    39241 gggataaaga tgcaggcaat cataagcggt tcgtttcctg tgttcattgt tttatgctct
    39301 gaccccggcg gagcatacag tgcggtgcct ggtttaattt tgtattccat gccttcaaac
    39361 catgctttgc cactaccgga catgaagatc ataagctcgc ccgatgtatc atgtacatga
    39421 aaatcagtac agccgccgtt tggagcaagc gttgacagga tgaaggtaaa atcgttaatt
    39481 gagcggtttg tatcgggtga tagtatcact tttaattccc gttcaaatgg cctgccaacg
    39541 gtggtaccgt tgattgttga aaaatctatg aacataatcc atgtacctcc atcattacaa
    39601 tgtgctatga tgaacctccc tgctataggg ggctttactc ttcatcgact catcgacatc
    39661 ataagcattt acctgccaca atgactatct cagaatttca attatggtaa atccagagac
    39721 ctgaccggat atatcatggt gccattacca tagcttctga agcgcgaaac atgcatatat
    39781 gtgatgctgc attatgagct gtcgccatga caggtttcct tcagcaccgt ttcctttaat
    39841 ttcaacgata gcatatatgc cttgctgata ttgtgtatta tgcaatatta ttgcataaat
    39901 gtcaacaact tttttggaga aagcataaaa attgctcatt tatcataaaa aaattagtac
    39961 ttgacactat agcgtgcatt gtcattgcat agtatgaaac ataaggagat ggaatgtggc
    40021 gcagttaatt caatcattag aacggggctt gcgtatttta gagatacttg gcaaatccga
    40081 taaaccgctt tcactgggcg aaataacgca attttttgat atcgacaaat catcaatttt
    40141 tagattattg agcacgctga cacaaaataa ttttgtatat caggatgctg aaacaaaaaa
    40201 atatatgctt ggtttcaaga ttatggagct ttcaggtgcg tttggcgagc aaacgaaaat
    40261 tgaaacgttt ttacggccgg ttttaaaagc tgtatgcgac aaaaccagac agaatactca
    40321 ccttgcaata ttacatggac aggaggtggt attcattgct gttgaacagc cgcgcaatgg
    40381 gataagtatg cacatatctg ttggcacccg cgaacctgcg catgccactg ctttaggaaa
    40441 ggtgctgatt gcttttacac aaaaaaatga gcaggaatta ctattacagg gtatagtgtt
    40501 tacacactat actccccata caatagttga tggtgatgaa taccgtaatc atctatcgtc
    40561 aattgttcaa aacaggtatg cggttgatga tgaagaatac cgattgggtg ttgtgtgtat
    40621 cgcagcaccg gtattcaacc accgtggcga ggttgaatac tcaataggga tatccggacc
    40681 aaaagatcag ataagcccac atattgctga ttacatcact gttgttcagg atgccggagc
    40741 ggaatgctca cgattactgg gcatgtcttc ccggtaacta tcactcatat tcaaaatacc
    40801 agtatgcacc ttcttttaca aagattaatg tcataccatt gtaaacggta accgttgcct
    40861 gtgcattgtt tattgttata tcggtaacgg gttgttgcaa tgcctgcaaa atgacatcat
    40921 caccttctat tgatgcaatt gttttttgca ggctcaggta ttgcggtatg gttagatttt
    40981 ttaaactggc ggcacttatc cggtagtgtt ttgcaaagga tgacagttgt gattgattca
    41041 ttgaacctat gagctgtatt gcgttttgta tgcgtttttt actgtttgta gaaagcagtt
    41101 gcgcaacctt tgccatgtca ccactggtat atgaatccga tagttgctgc aatgcctgct
    41161 ggggtgtaag tggttttgat gtgccgcagg caacaatgaa agaaatgagc agtaaatgta
    41221 tcaatatacg catgtaaatg aatctcctat cttttctact atcatattat caataagacg
    41281 tgttggtcca aagaatgctg ctacagcaat caccgattta ttgtttaaag agtcaacagg
    41341 ttgcaatgtt tcataatcaa ctatcgaaat ataatcaatc ttttttggct tacctgctgc
    41401 aataatgtcg gtcatagctg gtatgattgt gttagcctgt ataataccgt gctgaatgag
    41461 ctcttcagct tttttcaatg atcggtacat acaggttgca ttggttcggt catccccttt
    41521 taaatgaaca ttacgcgagc tcattgcaag cccatcgctt tcgcggataa tgggtgcaac
    41581 aataatgtcc agtggaaaat ttaagtcctt taccattttc tgaataatga cggcttgctg
    41641 aatgtccttt tgaccaaaca ccgaaacatc cgtttgtact atgttgaaaa gttttgcaac
    41701 aacggttaaa acacctctga aatggccggg gcgggccgac ccgcacaaaa agctggtcat
    41761 catttcaata tctatatagg taagcctatt ttgatacatc tcgctatcat ccggaagaaa
    41821 tatcaaatca acaccagcct cttttgccag tgcaaagtcc cggtcatagt tacgcggata
    41881 ggtatcatag tctctggggt cattgaattg cattttgtta acaaagatac tcataacctg
    41941 aaaatcagcg tgttgtttac aaatgtgtac aaggctcaga tgcccctggt gtaaatagcc
    42001 catggttgca acaaacccaa tccttttatt gttggtgcgc tcctttttaa ttatatcgcg
    42061 caatgcatca atggtatgga taatttccat ggttgtttta cctcaacaat taaatgtatg
    42121 tttttgcttt tttgcagcaa tgagcatcat gtcgcccaga tacaattttt ttgccaatcc
    42181 atcggcaacg gttctcagca tactgcctgc tttgccaata ccactgccat aggtagcaaa
    42241 ccgtgtaatg gtaaagccct ttaattgcaa caattttgca agcgataaat atgaaaaaaa
    42301 gtaaaggtga tgagggaaat tatagtatcg ccactgctta ccaaacaaga tgcggggcga
    42361 ccatatactg ctgcggcagg ttgagatata caacaagcca tcatcatgca gcaatgaaaa
    42421 aattttttca aggaacctat cggggtaatg gaggtgctca atggttgccc ataaggtaat
    42481 caggtggtag gtgctggtaa aattgaatgt taaaaagtca ccacaatagg catcaacatg
    42541 gagctgtgtg gttgcgtaat tgatgcagtc aggtgcaata tcaatgccac tggatttcca
    42601 gccgcgtttt gccagataat caacaaaata gcctgcagca catcctatat ccagagcata
    42661 ccgcgtatca ggcaatgttg attcaaattc ataaaagttt aaatcggcta agtttaatgc
    42721 aagcaatcgc gaaacctcgt tttgcatgtc ttttgaaaaa tagttgctat agccgcggtc
    42781 agtacgttta tcaaaatacc ctttgtaata ctgtggcata tcgttgtaaa aggggcgggg
    42841 atttacaaat tcaaggccgc aatgtttaca gcgcaccagt gtaaacatct cattgtcgcc
    42901 ccatggtttt gaaaaacgca acacggtgtc aaagctgttg cagatgatac agggaacatg
    42961 ttcatagttc atggattaag catcacaaaa aaatacaggg tattgcaaca actttttttg
    43021 tgcgatagtt actgtaaatg taatgttgtt gaaaaataaa aaaacctttg atttttaaac
    43081 tatatcctgt tacttttgtc ataaacataa gcataaagga gttacgtgct gctgtatata
    43141 ttttttacat cagcacactt tataaacact atacgtgcca tgaagatatc aaacaagatc
    43201 atcatgatta ctcaaaacag taatcttttt aatacagtaa aagatttttg caccaggaat
    43261 cattttgaca ttgaaactgc tacagagcta cattcccttg attttattat taatgaaatg
    43321 gaaaaaacaa aatcaacagc atttttaaga gcggctcttc atgcatttat ccgaaacgaa
    43381 ggtattccgt tttttattgt gactgattat ccagtagtag caacttcaca acatgatgtt
    43441 aatattcaga agatttttaa aacaatgctc atatccttta tgattattgc gcagggcagc
    43501 ggactgacaa atataaaagg taattttttt atcaatgtta ccggtggtga tataaaagca
    43561 ttaaaagata ctattattcg tccggaaaaa cttattgcac tgataaaagt aaacgatcaa
    43621 aaggtaaacg caataatgca tcactacgct gaccagaagg cattccattc tttattttat
    43681 ataaaaccag tggtatataa aacaaatgaa gaaatgttgc atgaactatc tacctatgta
    43741 gaagccatac ataagcgtca tgctctcatt gaaaagttga ttgaaaaaca agaacaggtg
    43801 cagggtaaaa acaaagatgc tgcaaaagtt cttgttaaaa taaataaaga taaaatagcc
    43861 gttgaccgtg aggtagttat aactcgtgat aaaacctatc agaaatatga agaaaaccat
    43921 atctatgtca ttggtgattg gtcaaatttc aacagccgta aggttgccgg taaagtgata
    43981 acagcaataa aagatggatt tgctgattgg aaacctggca ttgaagatca ggtttgcctt
    44041 caccttgaag aagcagtggt tgatcatact actgccgcga cgttggcaca gattatcttt
    44101 aatgacctga aagcgtatag caatataaaa atatactgcc atgaacccaa ttacaaaata
    44161 ctggaatctg ctgatggatt ttccatgata agaaaactgg tatttataca gaaggcttag
    44221 caaccaattg agtaggttat gccatctttc ccaacaatgg cagttaatcc atccataagc
    44281 tttttatctg gttttatgtg atacagagga ttagctttaa tgatattttc atttccgttt
    44341 ggaagacgta catggaaata taccggctta tttccctgaa aacgctgtaa taattttttt
    44401 attttttcaa gcaattctgt atttgttcct attggattaa taatgatatg caattcgctt
    44461 atctgagtaa gtttaaattg attaaatggg aaaattttct gtgcaatcat cctggagggc
    44521 ttttcatttt ctatttcaac ggtaccggtg atgacaacag gttcctggga tgttattaat
    44581 gtttcatatt ctgataatgt ttgtggcata aggagcacct gcaaactgcc tgttaaatct
    44641 tcaattgcag cctgagcaaa cttattcccc ttctggcttt gctttatctg cacgttgttt
    44701 actataccta ccagtgtaat gggactgcca ttaacattat tttccagatc ttttatggga
    44761 gtgcatgcaa atttttctat atcttttgca taacggctca aggggtgagc gctaacataa
    44821 attcccagca cttccttttc atattgcagc ttaacatttt cagtccaatc agcagcagga
    44881 gcaggctcaa tattgatacg tgaaaattgt ttgtcagacg ctccaaataa atcaccctga
    44941 ccggttgata tatcctgttg catattccgt gctttttcaa gcatgacatc aactgatgca
    45001 aaaagttctc ccctgtttgg atatacaggg tcaaaagctc ctgccttaat gagcgattct
    45061 ataacacctt tattaacggt cataagatct atgtgagtaa aaaactgcgt taaatcatga
    45121 aagctgtcca ctttctccct tgcttcaata attgattcaa tagctttgtc gccaacacct
    45181 ttaatagaga taagtccata ccgtatcgct ttaccctcaa ttgagaaatc tctatagctg
    45241 tgatttatat cgggtggtaa caccgctatc cctttagctt tgcaatccgt gtaatacttc
    45301 gtaatgttat cgatgttgtc cacctgagct gttaaaaggg ctgtcataaa ttctaatgga
    45361 tagtgtgctt taagatatgc ggtttgatat gaaactaaag cataagctgc agaatgtgat
    45421 ttattaaatc cgtagcgtcc aaatgttcgc atcaattcgt acatggtacg ggcaactgat
    45481 tcatcaatct ttttttgctt tgcaccctga acaaatttat cctccattgc atcaatgata
    45541 tccatcttct ttttgctcat ggcttttctc agtttatcag catcgggtaa agtaaagcct
    45601 cccattgcct gcgatatgag catgacctgt tcctgataaa caataacacc catggtatcc
    45661 tttaaaattg gttccagtaa aggatgcaga tattctactt ttttgggatt acgcttgcgt
    45721 tcaataaatt cttcaaccat ccctgaatca agaggtcccg ggcgatagag tgcaacaatt
    45781 gcaatgatgt catcaaagtt agtgggtccc aatttaagga gaatattttg catgccacta
    45841 ctttcaagct ggaatacgcc cattgtatcg gctttctgta aaagcttaaa tgtctttgca
    45901 tcatcaagcg ggatagctgc tatatcaata gcaatgtttt tatgtttttt aataagcttt
    45961 atacaggtat caataatggt gaggttggct aatcctaaaa aatccatctt taccagtcca
    46021 gccttttcca gactttcctt ttcgtattgt gatgaaatgc ttccatcttt ggggtcttta
    46081 tataatggca cgtgctccat caacggctct cgtgaaatga caacacctgc tgcatgtttt
    46141 cctgcagagc gcgtaagccc ttctaccttc tttgcaatgt caatgagctg tttgccggta
    46201 tcatttgttt tatataattg tttcagctca tccgaagcct gtaatgcctc ttcaagatcc
    46261 tcatgacgta tcaattttgt aagagcatta acctgctgca atgggatctc aagcgcacga
    46321 gcaacatcgc ggatagcacc ttttgatttc atcttgttaa aagtaatgat ctggcttaca
    46381 tggtcttcac catatttttg cttcacatac tctatgactt cgtcacgacg caacgcgcaa
    46441 aagtcaatat ccatatcggg catttcattg cgctcgggat ttaaaaatcg ttcaaagagc
    46501 aggttgtatt tcataggatc aagctcggta atctccagac aatatgaaac cattgaaccc
    46561 gcggcagaac cgcgccccgg tccaaccgat ataccatgct tctttgcaaa ctgaataaaa
    46621 tcccacacaa caaggaagta tccggaaaac tgcattcgct gtataaccga tagttcatat
    46681 tcaatacggc tcttcacatg atcaggaact atcgccccaa accttttttg tgcccctgta
    46741 ttaacaagct cggtaagata gctatctctg gtgtaacctt ctggaacctc aaaatttggc
    46801 aggatagcat tccccaatgg tatctggcag tcgatcatct cggcaatttt tacagtgttg
    46861 tacagtgcat cgggataatc ctcaaaaatg gtttccatct gctgaggagt ttttagataa
    46921 aattcagcat tggggaaacg catccgcttt tgatcgttca ggctttttcc agtttgtatg
    46981 cagagcaaaa catcatggta gtatgcatct tctttattga gatagtgagc atcattggtg
    47041 gctataagcc ctacattgag ttgggcagcc attgtaataa gctctttgtt aactttattt
    47101 tgttcgttca gattatggtc ctgcagttca agaaaaaatt tgtctttacc aaagagatcg
    47161 gcaaattcag atgcgcgttt atatgcctgg cttaccttgc catcaagaat gagtgaaggt
    47221 atttcaccgg ctatgcaggc agtgcttgca atgagccctg tgctatactg cgttagcagt
    47281 tcccaatcaa tgcgcggttt gtagtaaaat ccttccaggt accccagtga cgatagttta
    47341 acaagatttt tgtagccttc ataattttga gcaagcaaga ttaaatgata tgcattttca
    47401 tcattgctgc cccgtgtgct ctgtttatga aaccgtgatt ctggtgctat ataaaactcc
    47461 tgcccgatga taggttttat cccggcttta tgtgcttctt cgtaaaattt tatggttcca
    47521 aacatattgc catgatcggt aagcgctatt gcaggcatgg cattatccac ggcaaattgt
    47581 atcagttgct gtatgcggat tgcaccatcc agtatggaat attcagaatg gttgtgaagn
    47641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttgctgtatg cggattgcac
    47761 catccagtat ggaatattca gaatggttgt gaaggtgtac aaaatccata gctttctctc
    47821 attgtgttaa catactaaaa tttttcagct tgcgaaaatt tcaatatacc atgcatggca
    47881 tggcataata tttgtatgat tgtaatttcc atatgtgcca gggtgcgttt tgtacagcag
    47941 ggattcaaca gtaactatcg cccaacaaat acgacataat taaaatacca ctataaacac
    48001 tatagtggca ggtatttgca attttcaagc taaattttaa aaaattgtaa attgtatagc
    48061 gacaataatt aaaaaaacag gttgccagaa ttaaaaaagc aaattataat ggcaatgaaa
    48121 ttacggtgtg tgcctgcaat gtacgtgttt attttagcac agatagtgca ttcccaattt
    48181 atttttcagg agtaagctga tgattgatac aaagctgctt gacattttag catgtcctgc
    48241 ttgtaaaggt gatgttgaat atgatgctcg caacgaaaca atagtatgta ccgaatgtaa
    48301 aaagaaatac cctgtcaggg atggcattcc ggtaatgctt gttgaggaag cagaagaata
    48361 attttattgg ttaattacga ggtcaaaata tgtttgatgc aaaacatcct gttataaaag
    48421 tggcagggta tattattgtg ggtttttttg tgctgattat tattatttcg tttggtatgc
    48481 ctgattttct ttcacgcatg ggaatgaacc caaatactgt tgccaaagtc aatggcgagg
    48541 ttattggata catggatttt atccgctatc gagatacgca catgtttggc aaaactgatg
    48601 atcaaaaaca gcagcgtatg ataattgagc gtatgattca ggaaaaactt ctggtgcagc
    48661 ttgcaaaaaa agaggggatt gtggttacca aagaagagat aaagagcgtt atacgcaacc
    48721 gcttttcgga taatgcaggt tcatttaatg aaacattttt aaaaaatttt ttagaacatt
    48781 ttaaaattgg ggtgtccgac tactataaat atgttgaaga tgaagtatat gctggcaaat
    48841 tgcaaaacct gctgcttgct ggcataagtg tttcgccatg ggagatagtt actgattatc
    48901 gtattcagaa ttctaaaatt aaaattcagt ttgcattttt gtcaaatgca gaattggcaa
    48961 agcgttatgc taatgatatc gccgtaaccg atgaagaggt tgatgctgaa ttaaagaaaa
    49021 atccaaaaga attgaaagat ccaaaaactg ataggcagcg tataaaagaa aagcttgcca
    49081 atgcaaaatt agaaagcata aagcagcaat tgtcaaaaaa tattaatgac cttgctctgg
    49141 ctggtaaatc ctttgcagaa gcacagatgg tgcttaaagg tgttgtgtca tactctaatg
    49201 actttaaacc tggtgattta gtccgtgaac gtgatgataa agggcgaatt ctttacccca
    49261 ttcaggaatc aggggttttt caatctgatt tatttacttt aaggcagggt gcaacatcac
    49321 ggcttatagc tggttttgac gggctttata tatttactcc ggttgttcgt gttattcctg
    49381 gcatgcaggt tcctgaaaag gataaacaat cattagaaca aaatctattt tatgcaaagg
    49441 ctaatgcgtt gtatatttcg ctgctgacca gattgtttga aaagtcaaag attatacgta
    49501 atcagaaatt tgagatgtag gtattgagtg taataaaaac ttacagaatc catgtaacac
    49561 agtaaggggt actatatgaa tattaatgct gaatatgtta atccttttct tgaagcagca
    49621 agtgcagtat ttaaacagct tctcaacgtt gatctcagaa gaggtaaact ggtaatcaaa
    49681 gatgtccctc agccatcgca tgatgttgct atcattattg gtattaccgg tgcagttacc
    49741 ggacaggtag tgttcagtat gagtttggag atggtgggca agattgctaa ggttttaacg
    49801 ccaggacttt cggacaaaga tgttgccaat gaatggaaag atatcgttgg tgaagtagcc
    49861 aatatgatta cgggcaatgc aatgaatctt tttgcatatt ccggtaaacg tgttgagatg
    49921 acaaccccta ctgtcatcga aggtgaaagt tttaccatta cattgataaa gcaaacaaca
    49981 ttgggaataa atctttatag cccatttggg caattagaga tgaatgtagc gttaaagtag
    50041 tattcattgt tcagggaaat atatggcatc aatccgtttt atttcatctg ctactcgctc
    50101 tttaagtggt gatgaataaa aggcgctgta ggcagggtct tctgctcttt ttaaaaagat
    50161 ggtatatacc ttccgtgcca attcaatatc tttgaggtta ggatcagtgg caagtatttt
    50221 ccccaaccag taatatgcat catcaaccgt ttccctgtcc ggatattgtt taattaccct
    50281 gatcaatgcc tgcaatgctg cattcccctt tttttgagat atatataact gcgcagataa
    50341 aatcaatgca tcatctgaaa aatcctgatc agggtaaagc gatgcaacct ttgtatattg
    50401 ctcaattgca tcaaaaggtt tgcccatagc ctggttaacc cttcctgaca tgaacagtgc
    50461 agcagggtat tgtcctgatt gatcatttat tgccagtaac tcgttcattg cattagtata
    50521 cttttgttgc tgcatcaata tttttgcttt ccacaaactt gcctgatctt taaaagggct
    50581 ttgtggataa tctttttgaa ggcgctccag aagttgcaat gatcgctcat ataattgggc
    50641 attgtacagt tcaacaccct gttttaacaa aacttcatca ggtgttgacc cgcccttttg
    50701 ttgtgattca ttattagcag tagcgttgaa ttgcatgggt attgttttag caggtttggt
    50761 ttctgacagt atagtttcca gtattgcttc tttgtttacg ggctgatttt gttgcaatag
    50821 caataccgag cacagcagga atagttgtac agcgttcatt tttcaaatac cttttcgcgt
    50881 atgtccattg atttaagttc ttttttgtta tttgatggta taacttcttt ttcatcagtt
    50941 tttgcagatg gtgatgtagg ctttatggat ggttgtggtg aagttttctt cacattctgt
    51001 atggtatcaa taggttttac cactgcctca taatcactgc ttgtatatgg ctcaattggt
    51061 ttagcgtcat tctggggtat cagtggtttt tgtacctctt ttacatcttt ctgaatatcc
    51121 actggcgtgg ctggcatggt taacgcttca tcctgttttg ttggataggc tattttggta
    51181 tcagtggatt gttgacgttc ctccatcagg gcactttcaa gcattgattg aaacgatccg
    51241 ggttctcttt tcatctttat gtctaaatca aagacatcat tggttatctc agcgtatggt
    51301 ttaccggcag gccttgccga ttctttgcct aaatgatatc cccatagtat ggcaccaata
    51361 aataaaagaa aagaaatgaa aaaaaagcct tcttttattg attgctgcgt gcgaatatca
    51421 atagaatcaa tcatcctgtt tgttgtatgt acaatttttt gtaaaatgga cgctattgtt
    51481 ttcatgacat ttactttgct atactattct ctatcaataa taataataat attccctttt
    51541 ttcaagttat ttctggtatt attgcttgca ataatttttc tcaggtcagg atttgcaata
    51601 acagggcatg cattataaag gcgcactgct gcacagtagt acggtaattt gccagctttt
    51661 ttatgtctca gtgcggtatc atcagtatag cagtaggcaa ctgtaccgta ccggtatgcg
    51721 tcgcgcgaat caataaaatc tttaccaaaa atctccgtgc catcctcaga aaagattgat
    51781 gggaaaatca tgggcgtgtg ttttaatccg cgtgtgtcaa taatgagccc cgtataatca
    51841 gtagctggta attttatagg aagagcaggt atatccctgg atggcagagc ttccgggata
    51901 agttttaaaa gccttcccat cgggaatgtt gcaatgcatt gtgttgctaa tgtaccgtca
    51961 ggtttcaatc gcacctttat gtcggttgtc aataatccta attgtttctg ggtataatca
    52021 tcaccagcaa tgcagtcacc cagcgttcta tatggatcaa ttttaataat gccaagagca
    52081 ttgataagtg attcaatggc actatctctg gcttcatcta ttgaacggct tcgggcatag
    52141 tttatggaaa tggtttctaa ggtgttccta tcaacaggtg tgccatcgtc ggtaatagat
    52201 ggcatactgg aagcctttgc agtaatgagc tggtcggtcc agttgatttc aacgttatga
    52261 taaagctgaa cgaccttatt gctggcagag ttcaataaag aacattgtgc aattgaaaga
    52321 gctatcgcac aaataataat acagtaacat cggtagtttg gtcccataat ggatactttg
    52381 attattcgtt ttaacattta tcgtcatgca ggtggtcaaa atatagctaa attattcaat
    52441 attattttat ggcaatcctg tcaaatagtt ttaacagcag gtgagcagcc gataacgctg
    52501 catccttgat aagtgtatca atcttttcct gctcaagatt gtggcgtgca atgtagaaca
    52561 atgttttacg cataaatggt gaaatcagcg tatcaatagc atcatcatcc agaatgctat
    52621 agatgcacga agtattgccc gcttgtaccg ccctgtcaag attgtgtaat gcatctttaa
    52681 cttttgctaa ttgtttcata agttttgatg tgtcaatata tttcttgttt gatagttgtt
    52741 tataaatgat gtcttccggt gacgggaagg ctttctgcca ttgcaaaact aatgactgca
    52801 gcgttgcgta ttgtgtattg gcaatgacag cgccggcacc tgtattataa acagggatac
    52861 ctaccttttt tgctgaatcc tcaaaccagc ttcgatatag atccagaaca aaatcggtga
    52921 ttactgttgt gccatcattt gatggcacat attttatctt gcgtcttcgg ataatgttct
    52981 ggttgatagt atcaagattg ttatatcggt tagtgagcga tagccagtca tcattatgat
    53041 gagtgccgcg gcaatggatt tctcgtcctg tataggcaag gtcctggcct gcaagaacaa
    53101 ttggagagca tccggcattc agtaaaaggt caaatgcgct tgttgcaacc gaacctcctg
    53161 attgcacgtc accaatataa ccggtataat gttgcaccca atccattata ggtgttgatt
    53221 ctttttctat ttttccattt ggtgtgttat agtattttgc agtagtgctg aacatttttt
    53281 tattgaaaag cctgactacc tttgggcagc ttacaacatc tgcaagcagc aatggtttag
    53341 tactgcttcc aagaaaatgt tttatgctgt gtgtctgggc atccagtgtc ataacaatgt
    53401 gcggttgtat accatgacgt tgcagtactt tataggcggt atcaacgcat acaattaatg
    53461 ctttatcgta cgcctgccgt agtgcctcca gtgaatgtat caatgatggc ccggctgaaa
    53521 caatgatgcc gggtatacct ttaaatttgc caaacaacga ttgtactgga taagcagtat
    53581 gcatctgaac actgttgagc aaaatgtttt taatccataa cgcttcaaat tcaaatcgtg
    53641 ttaatagatc gctgatacgc gagcttatct gcttatgcag aattgctacc attgagtcat
    53701 aaaaatccct ttttagcata tacgatggac ggtgtataaa taaagctgtg cctctgaatg
    53761 aacgtatatc ccatgtttca ataattgcag caatttcttc ttcattatga acaattgaac
    53821 atagtgataa aaccagagga ttctctttct ttaccaggga aacaagctca ttatcttttt
    53881 ctattacaag aatgctatgg tcagggaaac gttgtttcaa tgctatgata tgatagccta
    53941 acgccaatcc gcatacaaca attatacctg ctctcccggg attaaattct tctgcagcac
    54001 gcattgcttc ttttaatgga tcatacgctg aatggagacg tatgacttta ttgccatcaa
    54061 ctaactgtaa tgatttattg ccgctttttg atgtggtaat ctggtactgt agcatcgtgc
    54121 atattatttt agtttattat atatatcctg gcatagtttg ttaagattgt caaaatcaga
    54181 tttggactga agcgtaaagt atcgggatac ttcattatca cagtaataat aaaagtaacg
    54241 gcgtgattcc acaccgcaca aaagattggc taaataggta acaaaaactg tgcccttaaa
    54301 cctgctatcg gctgacagtg gtgcatgatg atgtcgaata gcctcgatga ggtattctgg
    54361 cagttgccat tttgaagcga tgagctcgcc tataccggaa tggcttatcc cgattgatat
    54421 ttcctccatg acggttgatg tccgtattct acggtcagat accagtttag ctatccattg
    54481 cgttacatcc agatcaacag aaagcaaaac aattttacct aaatcatgga gcaagccgca
    54541 taatgaaacc tgttcaataa tagtattcat tttatatttt gtggcaatag ttctggcata
    54601 ggaggctact cggttgcaat gttcccagat ttgttcaaac ttctggaagc ggtcatccag
    54661 tatcttacga gcgcttatcg tgatgagcaa tgattctaaa ttttttagtc cgatagtcat
    54721 taccgcttcg cttagattat ttacccgttt gccggggaca aatccagccg agttggctaa
    54781 ttttaacaca tcagcggata acgctggatc acgcataagg gtcatggtaa tatcttttat
    54841 ttctacttct ttatttcggc aaagctgtaa tagcaccagt atgtgatccg gaaatgttgg
    54901 tatgtcatgt atggcattta taatctgttg ttttattttt gtagtaattg cttcaggacg
    54961 tagttgcaat ggcagtgata gcacaacccg tgtaaccttg ccatcagtgc caatgttata
    55021 attggacgga tctatgccgg cattttttaa tagtaagata acaagaacta tgccaagtcc
    55081 tgcaccttcg gtatcatcat aaacatcctc atacgcctgg gtaaaatcca gatactcctt
    55141 tgccttttca atacgggttt taatacggtt taattcttca gaatggattg gtgctggatt
    55201 ggcaatgata aaggtaacgc ctttatcttc tttacggata gacagggtaa ctttaagatt
    55261 gctgtttttt aactcatttt ccagcatatc taattggcca acaatatctt ttttaaaatg
    55321 ttcaatacca gttttgtaac tctgggggtc gttaatatca agattaagtt ttttgaaata
    55381 aatacgcttt gcatttgctt ttgttgcatt gacaataatc tcgcgtaaaa tagttatcat
    55441 catgtctaaa aggtaaatct gatcattttt agataggatg gtagccagca gggcgttgat
    55501 gattctggaa atagcttcat tgggatagta aaatgagatg ctaacaggtt catttttatc
    55561 aaaatgatca ataagctggg gtatattcac tggtatcata atatttttta ttacagtaca
    55621 atatataaaa cctattactt actatattat atagtatcgg ataatgcaaa tgtatacaat
    55681 aaaaaaagga ctatcaagta atttattttt ttataaaata tcaataggta ccattattta
    55741 caaacaaatg tttatccttt ttaatataat gtatactgca atggctactt ttctcctttc
    55801 attgtatggt tttttgttgc atagtatagt tgtatcaatg catctctgtc catcatggtt
    55861 ttattatggg ggatagtaaa ataaataggt atggcatcaa ccgatgctat cgtggttatt
    55921 ttttttatag catacaacgc ataatcccca actgcaatat cctgtaatgc aaattgtttg
    55981 gcaagcatgg ctgcatacgt gcaatttttg tttgnnnnnn nnnnnnnnnn nnnnnnnnnn
    56041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56101 nnnnnnnnnn nnnntcctgt aatgcaaatt gtttggcaag catggctgca tacgtgcaat
    56161 ttttgtttga agcattggga agcatgatgt gcacctttgt aactgcatgt ttttttaatt
    56221 gcgatgtcag gttaccataa tcgttaaaac ttttcaggtc cattatacag caggcacttc
    56281 ctttatcaag cagtactgta taccaggaac tatcgccacc agtataatgt attaccttgt
    56341 tttgcggctg gttatgatat agaagcaatg cgatgatgcc gcacagtata acgatattag
    56401 cctttttttt atataccaga gggattatta caataatcag gtatatgcct gcaagcagta
    56461 atgaatcaat atagatggtg ccatgcagtg aagcaaacaa ctgtgtaagc tttacaataa
    56521 tggtatacat atgatgaatt aggaatgatg caacgttagc taatcctgca tagcaaaaaa
    56581 gcggtatgct taatcctgca atcataaata tacttatcat tggtatgata acaagattgg
    56641 ctaaaaatgc tgtaaccgat agctcatgga aatgatatac actcaccggc atggtaaaca
    56701 gctgtgcagc aaaggtaatt gcaagagctg aagtaatacg tccgcaatgt tttaatccgt
    56761 tatgatactg tttgtaaaca agcaaaatac cagcagttgc cataaatgaa agctgaaagc
    56821 cggcgctaaa tatttcataa gggtatatgc acagtataat acaggctgca cagcacaaaa
    56881 gatttatgct gtgtttttgc atattcaata gtactgctgt tgctgcaact gcgcacatga
    56941 tggatgctcg caacagcgat accggctgat aggatatata aagatatatc agtacgcccg
    57001 atgacgctat catcactgaa atttttttgg gagctttgca aagcctgcat agtgcaagga
    57061 ttgtagccat aataatgcca acgtgaagcc cgcttgccgc taaaacatgg agtacacctg
    57121 cttctttaaa tgccatctgt acatgtggat ggataaattc tttattacct aaatacagtg
    57181 cagctaacaa tgatgctgca tcatgcgtca tggtacggta aagagaatgt tgaagtagtt
    57241 cctgcacttt tgtgcgatag tttttggcag caacggtatg cataataatt gaattgttgc
    57301 tcagataaaa tacagagctt atcccccgct gtgcaagata ctgtttgctg ttgttctcat
    57361 tgcgtgtggg gtaggcagta gcattaaata caatgacatc attgttatgt atggtatgga
    57421 catgatcaaa atgacagtat gctttatact gtccatttat gcctgtgcac cagatagtag
    57481 cg
//
LOCUS       DAOOJH010000007        57476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_641_length_57476_cov_11.334024, whole genome shotgun sequence.
ACCESSION   DAOOJH010000007 DAOOJH010000000
VERSION     DAOOJH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 57476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57476
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_57476_cov_11.334024"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            367..1662
                     /locus_tag="PLJ75_01860"
     CDS             367..1662
                     /locus_tag="PLJ75_01860"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPD03995.1"
                     /translation="MAKGDRVAIIDGCRTPFLRSGTAYREMMAYAICRHAVKGLVAKT
                     GIPNDMVDHGIMGSVLTDVKTTNVAREIMLAAALPNTIPAYTCTVACISANAAIISAA
                     NLIVNGNADCIIAGGVETFSDPDIKISKNYRQFIMDLTMFKRPRTIVGKLKLLKGMSF
                     KDFIVPEQPEIGEFSTQLRMGDTADRLAKRLGISQREQDAFAALSHNRAAAAWEQGIL
                     KKEVVPVVTPQGRLVDKDNGFRKGTRAEKLAALKPAFDKRYGTVTAGNSSFLTDGGAA
                     VLLMSETLAKKMRLKPLAYIKSYAFTAQDLWEELLLGPVFSIPKALAMGKLKFKDIGV
                     WEIHEAFGVQMVAVVQCLASDSFCKNRLGLSGKVGEINHDDLNIYGGSLSLGHPFGAT
                     GARLVTTCANRLQESGKRYGVVAGCAAGAIGNAIILERS"
     gene            1674..3797
                     /locus_tag="PLJ75_01865"
     CDS             1674..3797
                     /locus_tag="PLJ75_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPD03996.1"
                     /translation="MNAFTYCKPEVLNDVLVVTIDCPGKVNKVSSAMLNEMEVIINDV
                     KSKKYKGLAIVSGKDDNFVVGADLDELTSMKTEDEIRAYITKANSVLSGLEKLPFPTV
                     CVINGNCLGGGVELALVTDYRIATDSTNTVIGFPEIKLGLIPAAGGTQRLPRLIGIQH
                     ALPLIVQGSTIRARRAKRLGIIDEIVMPYGLKEIAVKKVLQLKGKRTIKRKRTFAEWL
                     LESNPLGRSIIFSKARTMVMRQTRGLYPAAVAVIDAVEYGYKTTIAKGIAKDSERFIH
                     LVQSYQSQSLVNLFFAMTDLKKNPFKKMEKNVSKLAVIGTGLMGSGIAFVSLPLCDTV
                     LLKDTSLDFVTRSMAQIRKSLEKRAQSGAIRNFDKEVMYGKLIPCDDYTYFKNTDLVI
                     EAVFEDLQLKQNLVKQVEAVCGDTTIVASNTSAIPISLIAKASKRPQNMIGMHYFSPV
                     PAMPLLEIIKTAKTADWVVATAVATGIAQGKSCIVVKDGPGFYTTRILAPYLNESVVL
                     IEEGVPMNDIDSAMLAFGYPIGPIALLDEVGIDVGAHVTIELGQMFTARGAAPSNVLP
                     LLLSKGFKGKKNKKGFYMYDSPKKKGMRLPNKEVYTVIGKVTPEAISVEEIQQRVSLM
                     MINEAAHCLQEGIISSPRDGDVGSVLGLGFPPFKGGIFRYIDHEGAGTIVDTMLQYYN
                     RGLERFKPAMILADMAKTDKRFYKD"
     gene            3802..4947
                     /locus_tag="PLJ75_01870"
     CDS             3802..4947
                     /locus_tag="PLJ75_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD03997.1"
                     /translation="MERSIQFTQEHALFRQTVRKFFEQQVMPFYDTWEHEGIVPREMW
                     NRCGQLGLLLPWLEEQYGGPGGDFLYSVILNEELAYSGANSFMIPLHNDIVAPYIYSF
                     GTEEQKMKYLPKCASGEIVLAIAMTEPEAGSDLAGMKTSAVSNGDSWVLNGQKTFISN
                     GILADLVIVAARSSKELPPHQSVSLFLVEDGTEGFTRGKPIKKIGLKAQDTAELFFDN
                     CRIPDNNLLGQEGQGFIYLMQKLQQERLVCALTSQAAMEKCLAITIDYVKERKLFGKP
                     LSKFQNTQFTIAEMASEIAAGRSFIDDLIHHHIQGHDVVKETCMAKYWICETAKRVAD
                     RCLQLFGGYGYCLEYPVSRFYVDARIQTIYAGTSEVMKMIVSRMMGL"
     gene            5043..5789
                     /locus_tag="PLJ75_01875"
     CDS             5043..5789
                     /locus_tag="PLJ75_01875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPD03998.1"
                     /translation="MNTVIRKGYTYATWLFIGVDTAFWGTISLFSWFVSPRGDLAHFC
                     MQNWSKNILWFCGIRYQVEGIENISKEHVQIFASNHQSFFDIWLLSAIIPVKFGWTAK
                     KELFKIPFMGWHMSLSGYIAIDRKNKERSRASMKEAAVKIRKGNRIMIFPEGTRSRTG
                     VLQPFKKGLFYLCIETGVPIVPIYIHGTYQRLKPETGDINPGKVYVMIGEEMPTDEYT
                     INTIEKAMNDLRDRILTLQNECLKRYGEAV"
     gene            5832..7118
                     /locus_tag="PLJ75_01880"
     CDS             5832..7118
                     /locus_tag="PLJ75_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPD03999.1"
                     /translation="MEDLKKAYRTIMDDHFPDTMTISFGNQTLIYKKRTWKINDDGQL
                     IEKGLRYGENPGQEAALYELVNGNLVLGDCEFISPGHSLVSGIDESMMLQFGKHPGKI
                     NLTDIDNALNIVKYLMKQPAAVIVKHNNPCGVAYGVTIAQAYNRANRADRIAAFGGCV
                     VCNRAVDKETAQLISQNYLEVLVAPDFEPGVMDILSKKKDLRIVRIQRIDRLEEYKQL
                     RFVDFKSLIDGGIIVQQSPVTIINSKDDLKPAQAEYKGQLYKIDRLPTPREYEDLLFG
                     WFVEQGVTSNSVLYVKDGVTVGIGTGEQDRVGVAEIAVQKAYTKYADMLCFDNYGISY
                     YQLALEVEKGLRKASDKIAIDEATKEAKGGLIGASMVSDAFFPFRDGVDVGIKQGISA
                     IVQPGGSIRDWESIMACNEANPKVAMVFTGQRAFKH"
     gene            complement(7131..7706)
                     /locus_tag="PLJ75_01885"
     CDS             complement(7131..7706)
                     /locus_tag="PLJ75_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04000.1"
                     /translation="MNYRVVKQFWINTVDNFFNFFYKIVDFLKIWVDVFWAFMDIWYH
                     FFAIFGNLFLYIYYIFLFIIDKSTESGTNILLTSRLPKQVPFRPQKAYVKDSYNPIPA
                     MYGVKNVAETVASSITPMRTTPKGTRTSIVKKLFTIIRDGIDIIVGLFKKPVKAIGNV
                     AARRMKPVKEEPHKTGSLIEEYIKEYEQKRH"
     gene            7927..9183
                     /locus_tag="PLJ75_01890"
     CDS             7927..9183
                     /locus_tag="PLJ75_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD04001.1"
                     /translation="MEYLTAIRPYIIKRLEQIKKADVLIGIPCFNNEATIANVMTMVS
                     EGLFTYYNDVRPVLFVSDGGSTDDTRDIAQSIELKPWQERIIQIYRGVAGKGSAFRAI
                     FEAAQILDVDACIVVDSDLRSISPDWIKLLLSPVLEKDFEYVAPVYTRYKYDGTITNN
                     IVYNIIRAVFGKRIRQPIGGDFAISKNLAKIYLDKPVWTTDIARFGIDIFMTVNAIVS
                     HVKICQANLGVKIHDAKDPAQSLGPMFVQVIGTLFNLMEGYEPYWKNIERSKPVPTFG
                     DINASEPEPIEVNLERLVNEYKLGFSQFHSLYQEMFSSDVYNALLSSSKLDVIHFSIP
                     VEVWVKVVYELSAIYHTWKINKVRLINLMVPLYFGRIASFINETLSMNSFQAEEEVEK
                     QAQVFENYKPYLIEKWQLQGKDIPFA"
     gene            complement(9191..11467)
                     /locus_tag="PLJ75_01895"
     CDS             complement(9191..11467)
                     /locus_tag="PLJ75_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD04002.1"
                     /translation="MKISEYLKQLVNKDKSITKSIRYALILTVILISLSFLLITCFYT
                     ANQMEQSMVNNYHEILLKQFEFIEYWLENKVEHIEKLSTASIINEYIESKQINKLIEY
                     LHQVIADQGEYYAITIMNDDGDVLCTTNSTFSFTMSAIYNQIKSTNDTAICKTIKIPF
                     KGNSITALPISCPIGKSIHYVKTKSPYIICFVNMDILFDSMRMINVGSHGNVYLIDKD
                     AQVILAANNNKPKNSYFNDYSLQDDRHSKNFKPVNKDGQLIASVKKCLSDFRSGIAQY
                     DNAYNDKVIGLWKWLSYFQYMFLIEISYAELLKPIYHTIIVYVMVSIAFAAIAIFFSI
                     TFSKNIKNKLSLFIKSFGEASVGNLSSEYPVTFNKNNIEIFMKQGETFIPYTSSKHCF
                     FEIGSIAKKFGNDIHCKFILEGQFKSCLQCSIYQSNAANEIDLLGAWFNAFMDKLRNI
                     IEDVSQIIQETFTASDEVSQSTQDFSENSNTLASSSEEIMATMEQLGAGFEEISKSVN
                     QQDVSISTMIATFDSLTEIMNDLNKIIIDAKENSNKFQTKAKEGQDMLQTMHESMTSI
                     QKSSQKIIDIIAIIDTISDHINLLALNAAIEAARAGEAGKGFAVVASEVGKLAEQTAT
                     SLKDIEKLILSSDNEIKNGIVNIQNTVSTIQSIIEGFSMIGTMMYEISNKMEIQNNTK
                     NQFVEEMTKVKDISSIINNSVREQQTASDNVIKSVSIINESTQGIAARSEELAANSIN
                     LKDRISLLQREIQFFKKV"
     gene            complement(11509..12462)
                     /locus_tag="PLJ75_01900"
     CDS             complement(11509..12462)
                     /locus_tag="PLJ75_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPD04003.1"
                     /translation="MNIRLLFRVCCCSLIFFIFFFCYILISCTGNPSATDVIEITKVN
                     HFITFDDRARGITATASTLPAVVISHETHSSASIQCKTCHHKQYNDERIKTCSFCHKG
                     TMGAEMYHTFCINCHTVKHKGPVTCNQCHKEKSSRLNEYNEIKKIYKKTFAFTENHHS
                     IHSKENIQCIQCHHNTKNNTKPESCSNCHAGHSKMVVLHNFCKDCHKKQGISISCEQC
                     HKGTQTNNIIKYETIILEKTGHRLPPIKFNHKAHIESYNTECIDCHHIGSLQKCSSCH
                     TKKDNKEIINLRSAFHQQCHECHRRSKGPLGCLRCHYEKVL"
     gene            complement(12712..13437)
                     /gene="rlmB"
                     /locus_tag="PLJ75_01905"
     CDS             complement(12712..13437)
                     /gene="rlmB"
                     /locus_tag="PLJ75_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPD04004.1"
                     /translation="MADQIIAGRKVVLEYLSSIEGDATLYISESAHGRILDVIADKAS
                     SLNIPINRVPRSWFDSHCQPHHQGVALKMSYRPHNTLQNRFHLPDIASSQGVIVACDG
                     ITDPHNIGSIIRTTEALGGNAVIVTKAHAPEITQTIIKASAGATAHIIVHYVANLASF
                     INDAKQSGFWIIGTSDSGTHPIHSIRQYKPAVLIIGSEGSGMRQLTQQLCDITVAIPL
                     RGKVSSLNASVAAGIVLWELLKE"
     gene            complement(13419..14846)
                     /gene="cysS"
                     /locus_tag="PLJ75_01910"
     CDS             complement(13419..14846)
                     /gene="cysS"
                     /locus_tag="PLJ75_01910"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPD04005.1"
                     /translation="MLALKIFNTLTRRLEPFIPGGIAKDSIAEYPPVTIYSCGPTVYS
                     YAHIGNFRTFIFNDFLRRYLKFRGYTVNHAMNITDVDDKTIAGALKEGVTLKEYTDKY
                     TRIFFEDCKTLNIEPVEHYPRATESIDAMIDIIAHLDKKGLVYEKDGSLYFSIAKFHK
                     YGQLSNVTSMDIKAGARYDADEYTKEDVRDFALWKAPKENELYWETPFGKGRPGWHIE
                     CSAMVRKIFGTTIDIHTGGVDLIFPHHENEIAQSEAAYDEPFVRYWLHVEHLLVEGSK
                     MSKSSGNFFTLRDLLNKGYSPRAIRYLLITAHYRKQLNFTFDGLTQAGQALFRIDNLI
                     ARLNDIKQEKPDNEEADKRCSQFIESFIEAVDDDLNIAEGIGTFFDFLHDINTLIDKR
                     MLGKADADTIMHTLNHIDTVFGFIFFHGAVTVNKEEIEALIEERNKARKEKNFTQADS
                     IRDELYSKGIIVQDTREGTRWLIKS"
     gene            complement(15203..15712)
                     /locus_tag="PLJ75_01915"
     CDS             complement(15203..15712)
                     /locus_tag="PLJ75_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04006.1"
                     /translation="MLFKRKKKVLPDVYEFTDDDGKKWLQTSTKDLLSSKELNDIIKE
                     IEKEKEEGEIVIARIDFKKVNKEYYERVVELEDTLKKRTDLLKRIIKESRETIDRKNK
                     KLKELIEYIKKLHLILAYYKLSPEDFNKIDFTKAYAKPSEIPAPQPSIEIKEKVVEYV
                     PVEEQIMQD"
     gene            complement(15776..17059)
                     /locus_tag="PLJ75_01920"
     CDS             complement(15776..17059)
                     /locus_tag="PLJ75_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04007.1"
                     /translation="MKKVRRAFFLSCLLMAMATSVVQAKTLLVVYPFCPDDLKNEATI
                     LFELLNKMTIENYSIENTKQIGSIDSFIKLSNEDSLSVIKKTARKRKNDYILFFEMSH
                     SVGQIFIESKLYSNTEGSFIATSFDWAYYHAIADIVKTIETKLSLIFQSKIIKITNVS
                     TLPDSTTNQITIHCETYGDNTSFAFVRSLFREGPYDTIATITTPSFTDNDVVPGIVYW
                     YRVQALYDSVVIDTSQPVSACIQAENKENLDLQKVLSLYKNPEEKPADDEQKETIKKH
                     LELLQPLYINSVKLRIIMQVVKSYVNKGQLIVLRNFDTTVMDTDNNCMYFIKKNSYVV
                     KLESKKPFNVLAASNFDLELLQRLSLNAIALGVPDNYIEITDDEGYTMQLPFYQAIGI
                     ATTYFTEYKHWASNTIMWNTSNKELNAKMKDYTNQ"
     gene            17368..18702
                     /locus_tag="PLJ75_01925"
     CDS             17368..18702
                     /locus_tag="PLJ75_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HPD04008.1"
                     /translation="MPKKHKQRDDIKAIYTHAIAAVDPYSAVCKFLQRKGNTLIIKGD
                     SNLVIDCDTIDAIYVVGAGKATARMAKAVEDIIGERIVEGIVVVKYGYREDLQYIQQL
                     EAGHPVPDENGLKASQKIIDVLGKAGEHDLIISCISGGGSALLPYPVEPITLAQKQDL
                     TQKLLKSGASIKELNVVRKHVSRTKGGNLALAAYPATVINIMISDVVGDDMDIIASGP
                     FVCDTSTYADALAILEKYKLTGTIAHAIIEHLEKGAHGEIPETPKDENIFKNVHNIIV
                     ASNSIACLAAYKKASELGYTPLFLSSMIEGDTGEAACFHGAIAHEIIKTGNPVQKPAC
                     IISGGETTVVVKGSGLGGRNMEFAMVMATLIENTSIVAASVGTDGTDGPTDAAGAVAD
                     GNTLNKAGRMGLDINDYMNNNDSYHFFERLGDLIKTGPTNTNVMDIRIILVV"
     gene            18849..19580
                     /locus_tag="PLJ75_01930"
     CDS             18849..19580
                     /locus_tag="PLJ75_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04009.1"
                     /translation="MKKKGLLFILSVALIFSLQLSPIVNAQQAQPQQAEKSEFTEATR
                     PEENYLARFHALERVEYLMKSNIDKIYMLKIIVTNYKDQGWEKDYEACYNGYKKGMDY
                     YYKRDVIYAQVELEKNRKDIYELYKKIAEVYKQRTQALLNECASKILDLSLDERSKSN
                     PDRNKVLFNNTARLRIAYGQYDDAQNMMDDHVYDTAVKHFRVAKSYAILILENIDPEN
                     SKGKYDLDKADNMNRILNQPQTQAK"
     gene            19672..20994
                     /locus_tag="PLJ75_01935"
     CDS             19672..20994
                     /locus_tag="PLJ75_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04010.1"
                     /translation="MIIVLFAVLFIFTFYTSCVQYEFPVYRVVVDPGHGGKALLPKDY
                     HGDRFDILSMKYLDMFREGAMYRQYYEHIYTYEIAVRIEKILQLTLPDGDFKKFCSIV
                     QKYTNKPFERVCIQSFVSRQPSLNSQMLHKDPNAPYRLFDSVFPDGTLQEGRISYINS
                     LHPHMVVSIHFSLNASRYYRGMNAVIAAPYSFMYKGLQYLQGNGGNRSFFYNSNYADW
                     FTETEDKSSFFWYCNDVIQYFMGYRMTNNYQPDIEAFRGYRYNMVQWIYNDPPGWAHI
                     AKYHPAKTAYADNVKQFIPGNAFFKREQSMYEKYRRDGGYEGYGGDNLYASNEIIRYV
                     LYNLYLQGVRHKEQRLAPPYISIWSIPLHVNAINAYMELGYLARPYTRYILDNHLDDM
                     AEGIAAGIYSLFAGIEVSNKIPCAPYGKKIDLKKYTINKNNNYFTIVR"
     gene            21016..22281
                     /locus_tag="PLJ75_01940"
     CDS             21016..22281
                     /locus_tag="PLJ75_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD04011.1"
                     /translation="MKKLSIIAALIFFQTLVYGDNISQRFIAAADKAGKAVVSIQVFV
                     VEKGHYQKVGYASGTIISSKGLVVTNYHVVAKGTVYQVNLCDGTECDVEPFADGSLFK
                     ADEKTDIALLKVKHSAGLPLTPIEFEDKKVFPGQWVMAIGNPFGLQQSVTAGVVSSLG
                     RSDIGFADIEDYIQTDVPINPGNSGGPLISLDGKLIGINTAIRTQSGGYQGISFAIPA
                     FIVKQVVGELVQYGRVRRGWLGFLVQEKMINNNSPVHVEIISIVDGSPAYKAGIEVGD
                     IVREVDGIPIKKISDLMKAIHSKQIGSLVNIVIARDGQLLNFDFVLQEKSKISRVNYW
                     INVLQQSYGITIEDTLYGAVITGVNQFGIAYNVLKKGDIIQSINDKQVTSLDDIIAIV
                     KKTKGTIENCVVLRSGKVIRCYFNLDGTE"
     gene            22285..22722
                     /locus_tag="PLJ75_01945"
     CDS             22285..22722
                     /locus_tag="PLJ75_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04012.1"
                     /translation="MNKRIILFIIAAFMSMCIQSFSDEKKAEVILDNEFYTSLQNSDA
                     IKRIRTFDMLKDVIVKGTGNVVKVEVADVLKKKFRIVLSLNLHGIQILYYCYTDQDEY
                     FDLLQPDDLFEFSGQFVVATPLNVRFDTFIIDVLLEEGAVVVH"
     gene            complement(22725..23342)
                     /locus_tag="PLJ75_01950"
     CDS             complement(22725..23342)
                     /locus_tag="PLJ75_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04013.1"
                     /translation="MKNIAKTADIKTNTPVRVYKKGYGYALLTVIENNELFLLCKSSE
                     DFLSHVKENDTVECYLWPYPDGSYDFNTTILGKINAPFPIILLQHTDTIVYNPSRRCL
                     KAQVNIPFTFFTFSINTSRSFSSEEIQWHEGIVKELTDREALLQTTTPLEHYVKGHLT
                     LGTKKIDITGKVTASDDTLYDITFVGLDDADRISILDYVFTVYRE"
     gene            23670..23963
                     /gene="rpsF"
                     /locus_tag="PLJ75_01955"
     CDS             23670..23963
                     /gene="rpsF"
                     /locus_tag="PLJ75_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPD04014.1"
                     /translation="MNKYELIVILRPTDKLDILKEKLRDILKKNQVVIESDEPWGVRA
                     MAYAIDRANDGYYEYFIVQIDPAQISAIIAEFRLQPDILRYLFVRLPNLSKTA"
     gene            23970..24392
                     /locus_tag="PLJ75_01960"
     CDS             23970..24392
                     /locus_tag="PLJ75_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD04015.1"
                     /translation="MPGDLNKVILVGRIVRDPELRYTPGGAAVVSFSIANNRIYVKNN
                     EKQEVVSFFNCVAWGKLAETVANYCKKGQQLALEGRLQQRSWDDKDGNKRSTVEVVAE
                     SLQFLGGKPHAETSPSVDSMETGDMGSTEPFSDDDIPF"
     gene            24429..24776
                     /gene="rpsR"
                     /locus_tag="PLJ75_01965"
     CDS             24429..24776
                     /gene="rpsR"
                     /locus_tag="PLJ75_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPD04016.1"
                     /translation="MSEEINQGETIQAQENIIPEDTKSSEVARHSEDKALSAAVQVQR
                     VPKFKKKVCRFCEDPNVAIDYKNPDLLVRFITDRGKILPRRITGTCAKHQRHLARAIK
                     RARTIALLPFVVK"
     gene            24792..25640
                     /locus_tag="PLJ75_01970"
     CDS             24792..25640
                     /locus_tag="PLJ75_01970"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPD04017.1"
                     /translation="MELIVHLIAGIALCVITAIVVYKLKYKALAIALVMVLSALFLGL
                     SYFYNEMIGLELIITGAVFGYVAKRFSFQRYFITSVTIVWLLLLIIHYILYFVKGIDA
                     ISGNQKIIEEIINASSIPVTEKQVLSQQLEDFLPLMRVLMPFSYFFSSAAISILVYVF
                     VFAFFVRFKFLQTEDNTISLFRVDEWVVFLLIASWLVVLLGKELQTVWAIALNIGLVL
                     CVVYTIQGISVILFVFEKKQWPKLYGFLILFVTILLGFQVMFFVITMLMGIGIIDFWM
                     NFRKIA"
     gene            25666..26109
                     /gene="rplI"
                     /locus_tag="PLJ75_01975"
     CDS             25666..26109
                     /gene="rplI"
                     /locus_tag="PLJ75_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPD04018.1"
                     /translation="MRVILQKDVQNLGEAGDIKEVAEGFARNFLLPKKLVIPYNESSA
                     KAIEHQKKLVKIKKEKRKKEGQKLAEALSSVALTIGAQVGEDDKLFGSVTAIDIADKL
                     LQMGYTIDKRKIVLPEPIKKAGEYEVKIKLEEGLTATIKVSVVKE"
     gene            26114..27448
                     /gene="dnaB"
                     /locus_tag="PLJ75_01980"
     CDS             26114..27448
                     /gene="dnaB"
                     /locus_tag="PLJ75_01980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPD04019.1"
                     /translation="MLDVGMPPHDTEAEAACLSSILLSKDALLKVIEILQVEDFYIDA
                     HRMIYEAIRELEIKSVPIDLITIKQRLIDKKQFEKVGEDQALVNIYRTVSTSANAEYY
                     ANRVKELSLRRKLIETCTTIAERSYDTTLDTKVLLDDAEKDIFLITQRRITSDFISLA
                     NVLKETLENIGHMYETKRPVTGIAGGFTDLDAMLTGFHPSELIIIAARPSMGKTALAL
                     NIANHVALREKQPTLFFSLEMPATQLATRILCIESLIDMQHVRTGQISNEQLRKLVDI
                     SGRLEKSPMFIDDTPGLSIFELRAKARRFAQKQKVGLIVVDYMQLIVANSRLERHQQI
                     AEISNALKQLARELSVPVIALSQLSRAVESRTDQMPQLSDLRESGAIEQDADVVLFIY
                     REEKVKKETERLGIADIIVAKQRNGPTGRIELRFWDKYMKFGTVEKFREEAE"
     gene            27450..28235
                     /locus_tag="PLJ75_01985"
     CDS             27450..28235
                     /locus_tag="PLJ75_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD04020.1"
                     /translation="MNNAEIIFLRPVMKFFESVGNHTLFFAQTVKWIFKPPFDTKNIL
                     LQMLEIGYKSLPVTCITLFFTGMVMALQIGRAMDSLMSGSSIFIGSAVSIAMFRELCP
                     VLTALLLSGRVGSAIAAEIGTMRVTEQIDALITLDANPIQYLSVPRFMACLVMTPVLV
                     VITDVVGSLGGAFIAYFNLGITIDKYIENVFNYLQVMDFLTGLLKSVFFGIEIAIIAC
                     YQGFNTFGGAEGVGKSTIRAVVASSMTILISDYFLTYLLQFIE"
     gene            28252..28896
                     /locus_tag="PLJ75_01990"
     CDS             28252..28896
                     /locus_tag="PLJ75_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HPD04021.1"
                     /translation="MKSLKKRKEEFIKLLQKNRIPKRIVDAFAIVNQEDFFDEIYSDA
                     LYGTQPIPVAPGKTGDEPLTLARMIHYCGIKKDFHVLEIGTGSGYSTAIMAHLAQSVT
                     TVEIDEELALLAKKRFSRLKLTNIRVFAGDGTTIDESSGIFNAAVIFAGCTKRPLMLL
                     HVMKQDGVVVFPMGPAHQQQIALMLNHRDEKVEGVFPVVFKEFCTFMPIAGIYG"
     gene            complement(28897..29241)
                     /locus_tag="PLJ75_01995"
     CDS             complement(28897..29241)
                     /locus_tag="PLJ75_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04022.1"
                     /translation="MVEIILYILSFGAGIALFLYMLSLYHHLQKKTSQSAKKETNKAA
                     TVMPAKTVDKNFTPASMKERTCPVCGSKLSRYEALYASKIVKDSTKILIYGCRYCYKP
                     DEDPDKIKKSDI"
     gene            complement(29247..29969)
                     /locus_tag="PLJ75_02000"
     CDS             complement(29247..29969)
                     /locus_tag="PLJ75_02000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04023.1"
                     /translation="MNEYHHLAPYYDYMLQHVNYNEWYRYLRNVMCKYISNPRTVLEL
                     GCGTGKFGPKFSMDDYEIYGMDKSIAMLSIAKTRANKNFHIFCGDITCFALSKTVDFI
                     FSVHDTFNYLLTYDDFAKALRCAYNCMSYNSIFLFDITTQYNIMKNFHRRLFAYTVKG
                     TDITWYNEYDEKSKMVYTKLTFATQSRTIIEEHLQRIYTIDEIIPIIKKCGLLVVDIA
                     GDYTMKPVTDHTIMINVITKRA"
     gene            complement(29966..31096)
                     /locus_tag="PLJ75_02005"
     CDS             complement(29966..31096)
                     /locus_tag="PLJ75_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPD04024.1"
                     /translation="MHDSSWISLNGGSTITTPHGAVTLFAHHHDSDSFPITTAQLYLD
                     DYCRIHHIEPLHLHECIFFDIETTSLTSGTGNYAFLLGMGYFANNVFTVEQLFMREYA
                     DEPALLYYFADRVNNYKAIVTFNGKTFDIPIIKTRFRINRIPGFPVSMPVIDLLKPAR
                     SIFKSIYASCSLKSLEELLLDVTRTDDIPGYLIPDVYFTYQQTGFDPRIINVIEHNRI
                     DITSMVLLLVFFNTLYQMLHAKQFHQVPSNLYTNIAKHLYSRNIQLFVELVEYAGFAL
                     LHNEHSLFKKYSTALKRLGYTDRALAFWEKHTNLFSLEELAKHYEHAVKDYEKALEYC
                     SRAIVIVNVSPDDNELNSRYKARFAHRINRLQKKLQWKHIAP"
     gene            complement(31110..33389)
                     /locus_tag="PLJ75_02010"
     CDS             complement(31110..33389)
                     /locus_tag="PLJ75_02010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD04025.1"
                     /translation="MNIQQFIEYLTNYEPFIKQVAAWHTIPAKEAEYALYPDNCDASI
                     IAVYKKRGIEKLYTHQRSACDAVQRKENIVVVTPTASGKTLCYNLPVLNHIVKHPDAR
                     ALYLFPTKALSQDQLSELQEIIGFAGLSVKTYTFDGDTPQSARKAIRTAGNIVITNPD
                     MLHSGILPHHTIWVKLFENLQYIVVDEIHTYKGVFGSHFANLMRRLKRICSFYGSNPQ
                     FICCSATIHNPKELAETIFELPFTIIDNNGAPRGKKHFVIYNPPVVDKALGIRASAIK
                     EAASIGSLCVKNNVSTIIFCRSRLRVEIIATYLKEKCNNYSIAAYRGGYLPNERRQIE
                     KGLREKKINAVVSTNALELGIDIGMLDCAISVGYPGSIASLQQQSGRAGRRGAESLAI
                     MVATSSPLDQYIARHPHFVFENNPETANINPDNLLILMDHIKCAAFEIPFSTDELFTH
                     HHLTTKEMLHYLASKNILKESGGKFHWMSDIYPANDISLRSASQDNVVIVHNDNHAEV
                     IGQIDYPSAPTMIHDDAIYIHQGQQYYIDKLDWERKIAYCHEVESDYYTDAEEKTDIH
                     VLEEFNNKSFSIATLSLGEVNVRTKAIMFKKIKFGSHENIGWGKINLPELEMHSTSVW
                     CSFTDECVQQFEREEAGEVFIGLSYILRTIAPLYTLCDYGDIRVVFENLSHFTQRPTI
                     FIYDAVPGGVGISERIFAIFGLIAQASKEVIAACTCTNGCPACIGPMITRTGIKEKII
                     ALLEILHNEFEGQTGHILH"
     gene            complement(33392..34240)
                     /locus_tag="PLJ75_02015"
     CDS             complement(33392..34240)
                     /locus_tag="PLJ75_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPD04026.1"
                     /translation="MAMEEQSLELSHKAILQPLLSQIQLPISEYSFANLYLFRKEHNY
                     SYISGDIVAVRGITYDGTSYIMPVKPLDCEYAEKLTQLLLPGSCLFPIADDWLHFFTE
                     KGLSYYYNDGDSDYVYTKEKLATYPGKKLHGKRNLLYQFTAKYSTTVLPLTSERINDA
                     LYVLDEWKKESGTSADVSACSEACMLLDELNLCGSIYYVDDKPAAFIIGEAITVDMFA
                     IHFAKAITSYKGIYQYLYNHCANALPANYKFFNFEQDLGVEALRFAKRSYQPDMIVTK
                     YRVSYP"
     regulatory      34397..34534
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            34550..35947
                     /locus_tag="PLJ75_02020"
     CDS             34550..35947
                     /locus_tag="PLJ75_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPD04027.1"
                     /translation="MQFFLCPCPHKGVFMSFRKKFLKAVSPFQILVLGYLLITVSGAF
                     LLSIPFASKDGQWHNYVDSLFMAASAISTTGLVVVDTGSFYTLFGQLVLMTIFQIGGI
                     GYMSLLMFVLYLFDIKTSITVQLTAKESLSGPTLRILGKFFLVTVIFTLIFEASGAIV
                     LALYWMQKYNSITAVYYGVFHSISTFCTAGFCLFPDSLMSYKDSIIINGSINFLSLAG
                     GIGFIVLYETFAFVSKRLRGIKVKHVSVHTKVVLLTTFIIVITATLLLLVTEHWDYST
                     SIIQRFVYSSFQVISASTTDGFNTIDIGGMSSASIIIIMLLMFVGASPGSTGGGIKTT
                     TFAIICMYIVSLLKGRSNGVVNIYEREIPTESINKALGIFIMFIIVTMIDLISMSMTE
                     KCNFENILFEIISALGNTGLSTGITSQLSITGKVLLTVTMFIGRVGPITFGFFILGNK
                     KVQIVHYPQEDIFVG"
     gene            36307..36570
                     /locus_tag="PLJ75_02025"
     CDS             36307..36570
                     /locus_tag="PLJ75_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04028.1"
                     /translation="MIQNKHDQAAAIEEVSASIEELSAGNENVKLHTDRQFDRIAILS
                     QIMITASDLIKEMIDLTQKALTRMQDIFKKAQSGEASLALMSE"
     gene            36571..36933
                     /locus_tag="PLJ75_02030"
     CDS             36571..36933
                     /locus_tag="PLJ75_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD04029.1"
                     /translation="MGTIAKSSGEINGIIAIIINDISDRINLLSLNAAIETARAGDHR
                     RGFAVVAGEVSKLADQTAVSIKTIGSLIINNDREIKTGMQNVSQAIGTINSIIKDIDV
                     IVSHISAISDTFKAGRGK"
     gene            36958..37164
                     /locus_tag="PLJ75_02035"
     CDS             36958..37164
                     /locus_tag="PLJ75_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04030.1"
                     /translation="MSLLSEQIKIAMDEQKSAISEIAKTVSSINELAQENTAKGLDIT
                     ETSRALVGKVQSINKEIDEFSSGT"
     gene            37392..38021
                     /locus_tag="PLJ75_02040"
     CDS             37392..38021
                     /locus_tag="PLJ75_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPD04031.1"
                     /translation="MPFYKFIPHEIKFFDLFDRQADKMVEAVICFKEFAEKGIFDDSC
                     IERMRLLEHQSDSIAHDIIDSLNRTFITPFDREDIHSLTNKLDDVVDLIYAISKRMRL
                     YKLKGKNADVQEFSRLIMQSVQSLSLAIKGLRNHRQPQIIQQACIEINKIENISDQLR
                     DVVIQRLFKSKKDPIAIIKWKEIYELLETVLDVCEDVSNIVVSILVKQG"
     gene            38028..39029
                     /locus_tag="PLJ75_02045"
     CDS             38028..39029
                     /locus_tag="PLJ75_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPD04032.1"
                     /translation="MTIYIFFLIALALLFDFLNGFHDAANSIATVVSTRVLSPRYAVL
                     WAAFFNFVAFLFFGLHVAGTIGNGIIDVSIMDNTVILATLTGACMWNIITWYLGLPTS
                     SSHALIGGLIGAALMKVGTKALVLDGIIKTVIFIFLSPIVGFGLGLVFGTAVYWIFRH
                     SHPAKVDKIFRKGQLISAAFYSLGHGGNDAQKTMGIIAGLLFSNGLMQVAEGQRFQDA
                     IPLWVVLACHSAIALGTMFGGWRIVKTMGQRVVKLRPVDGFCAELGAAFNLYVITSYF
                     GIPVSTTHTITGAIMGVGTLRRFTAVRWGVAGQIVWSWILTIPLSALIAAITYSIVT"
     gene            complement(39049..40911)
                     /locus_tag="PLJ75_02050"
     CDS             complement(39049..40911)
                     /locus_tag="PLJ75_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD04033.1"
                     /translation="MQLIEIIKELEATDKNIPSLEIDSYRKHFSKSVFYAKNYNKEFP
                     DKFVTITGEEFLTLQYNIASALLAYGVEKDTIVGIFSRNNLYYAATVFAVMSLGAIVM
                     PIYPTILQDELEEILRDSGCRTIFVGDAAQFQKSFAILNKVQSPLRRLITYFDSKTKH
                     EQLVTFDELIQFGEEKAHYEEINRAIEKINSEDYAALMYTPGTSGDPRGVPLTHGNFI
                     SQHVVIPIFKFSDADIRLAHLPFSHVYGFAADLLISSFTGAPMAISTTLESEEIYRNM
                     IDIKPTVIATVPRMYEKLYINTIHTIRRFNPIKRFIYFSSINIGRGCYIAQTSGKTIP
                     KGAALFNKLLSPITKSIQKLMHMQQVRLLFSGGAPLPIDVSIFFGGIGLPIIEGYGLT
                     ETSPTVTINRIEKNKPGTVGYPIPGAEVKISDEGEILIKGPMVFKGYHKMTPEERLEV
                     FTASGYFRSGDIGTIDEDGYLRITGRLKDIIITSQGKNIVPLHIEKKFEAEPLINYIC
                     VVGDNRKYLSALIVPNFHLLRQYAREHGISYKTDEELVSHHQIQTLFKTRINEICQTV
                     APYEQIKKFTLLAHDFTVKTGELTPTFKFRRSQIHEKYKDLIDLMYPKSDTTRL"
     gene            41277..42497
                     /locus_tag="PLJ75_02055"
     CDS             41277..42497
                     /locus_tag="PLJ75_02055"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPD04034.1"
                     /translation="MKTANIPELIEEIRNGRMIILVDNEDRENEGDLVVAAQYCTPDK
                     INFMATHGKGLICIAMTQERLQELGLPLMVPENSDKFCTAFTVSVDAKEGTSTGISAY
                     DRAKTVHALIDPRTKPQDLNKPGHIFPLTARKGGVLVRAGHTEGSFDLTRLAGLYPAA
                     VMCEILNDDGTMARRPDLDAFAEKHNLKIGTIEDLIKFRQHKERLVHRVSEAHLPTEY
                     GDFKIIAYETEVDNATHVALVKGEVDGRENVLVRVHSECLTGDVFGSHRCDCGSQLHQ
                     ALRMIEKEGLGTLVYMRQEGRGIGLSNKLKAYHLQEQGYDTVEANIKLGFPADLRNYG
                     IGAQILVDLGLSTIRLMTNNPKKVIGLEGYGLTIVERVPIEIPPIKENYRYLKTKKDK
                     LGHLILQAQEDKKS"
     gene            42494..43402
                     /locus_tag="PLJ75_02060"
     CDS             42494..43402
                     /locus_tag="PLJ75_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04035.1"
                     /translation="MKNGQAPTIVVVDDDEVIRYSLQKKLSRLGYNIISLSKAEDALF
                     LLKNDEREIDLIITDIKLRKMDGIELLRHVNVSENPVPTLIITGQGNIEDAIKALRYG
                     ALDFIRKPFDMNEVASVVRNILRRRQQHSLENTLGQYIVDDHRTLVIPIDETIADLVA
                     YELTKHLSYAKFCNRSTAENISMALLEALSNAMYHGNLEIPSEVREEHGVKGFHDEIQ
                     KRLQDSQYNQRKVTIRYSYTGEYVEYIIEDEGKGFDYKNVADPRDPENFLKNSGRGLF
                     IIQVHMDEVEWDNGGRRIRMRKYKTL"
     gene            43415..44449
                     /gene="mtnA"
                     /locus_tag="PLJ75_02065"
     CDS             43415..44449
                     /gene="mtnA"
                     /locus_tag="PLJ75_02065"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPD04036.1"
                     /translation="MYYTIKWDDTAVSMLDQRMLPVQEIYRHYTNYHDVADAIRSMVI
                     RGAPAIGIAAAMGIALAAVKNKLSVHDEFKTEMVRVCDEFAQTRPTAVNLFWAISRMK
                     TIIEKATDIQHCKEALVDEAKKIHQEDIAINKTIGENGQKFLDDGMTVMTYCNAGALA
                     TGGYGTALGVIRSAIEHGKKIKVYACETRPYLQGARITAWELYKDGIETTLICDNMAG
                     YFMKKGEINCVIVGADRIAANGDTANKIGTYTMSLLAKAHAIPFYVAAPLSTLDTNTI
                     NGDSIIIEERSIDEVAYIQGVQIAPEGIFIKNPAFDITPADNITAIITEQGIVEHPAA
                     TGIAPLFSVR"
     gene            44689..44976
                     /locus_tag="PLJ75_02070"
     CDS             44689..44976
                     /locus_tag="PLJ75_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPD04037.1"
                     /translation="MNKYYIYMITNRNNNVLFIGVTNDLKRRVFEHREKLVEGFSSKY
                     NCSKLVWYEQTNNIGSAILREKQMKKWKREYNNNIINEMNPEWNDLYETIL"
     gene            45052..45480
                     /locus_tag="PLJ75_02075"
     CDS             45052..45480
                     /locus_tag="PLJ75_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04038.1"
                     /translation="MRQAISIIPVILSMVLYGTIGVARDIPYTLEDRERLIRVETKLE
                     EGYKALHSRIDAVDKRIDAVDKRIDDVKGLLYVLIAAIFAQTVGVVGFVVWDRRTALS
                     PAINKTKEIEEKEDKIITALKQSAMKDKNIAEALRKAGML"
     gene            complement(45497..46261)
                     /locus_tag="PLJ75_02080"
     CDS             complement(45497..46261)
                     /locus_tag="PLJ75_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPD04039.1"
                     /translation="MNDTLIVAFAFIIGASWGSFLYTFIIRYTNGMYTNNPCHALTYP
                     SHCPHCHKKIKPVYLIPVIGYCLTKGCCAYCKKPVSLRYPLSELFCGIVAVIAVLQFP
                     LAIAPVLFVAFTTSVSIAIIDTITMEIPDLLVLIILIAGIIITAIEGEVVSHIEGAAL
                     MLVVFILPLVIKKGGFGGGDIKYAVAIGFFLGFSETIVALEIALVSGALFGILFAVIK
                     KKTLKAKIPFAPFLTLGFIAALLYGKEIILLYYYYI"
     gene            complement(46444..47124)
                     /locus_tag="PLJ75_02085"
     CDS             complement(46444..47124)
                     /locus_tag="PLJ75_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPD04040.1"
                     /translation="MKTVHHTIIITIALLLFIFIEHEHAFAIGEFSIGLNAGLTQDPN
                     HLEPAITQWNTQAKTTASDVDQISIPYSFVWGINVRYQFNYFLFRIGAHFTSPYSVRG
                     EADSTRIKISTYQFGLPATVGLIMPLKQRAYFFLGAGLTYNYAYLEIKQTNPDFKVRY
                     ADSIPGFHYIMGAEAPLTSKITLSVEWMHQEGRSIPIKDEVSGTDKRTISVKGDFLLF
                     GVNYYIGM"
     gene            complement(47136..47888)
                     /locus_tag="PLJ75_02090"
     CDS             complement(47136..47888)
                     /locus_tag="PLJ75_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04041.1"
                     /translation="MYYKLRKILFMAAIALAPIAVYASPFNQAISFYAGYIPSLGDNL
                     YSYEQENTFGSTNGVDGINKSLSGFNTSQIHRLIGATAGINLKIVMYDFFALRIGANY
                     FKNVWGGKGKTVYDDGGTTRLLECTYRYDGFDIPVTFGIVIPFYKDMKITFSCGIAYA
                     WGRYRNKFESDAPLLYRGEFKGHDYPLVLNVEGEYFMDEKVALATSLSYYRGTTTVIK
                     DSKQGDGNTDYAPLDFTGYRFLLGISYYFKPI"
     gene            complement(47878..48534)
                     /locus_tag="PLJ75_02095"
     CDS             complement(47878..48534)
                     /locus_tag="PLJ75_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPD04042.1"
                     /translation="MVKKAIILTTLVIVLHTGIVTGYAQRYAGIGIHVGAQHDVGDIT
                     TFYPDAKTDPQNSILFGIAVRLSIPYFFIRTGADTSFTLNKGKVLEDEATVGVKSYNV
                     KYAMIPLYAGLQFPLLDKGVFYLGGGISYFYGTGNVRLSDGSSKDIDATCLGTGFLAG
                     IQLHITSSTSLYMEWMYIDAQSDPVITTTGTLNPNGYEDLFINYGGHRIMLGVMYYVL
                     "
     gene            complement(48624..48935)
                     /locus_tag="PLJ75_02100"
     CDS             complement(48624..48935)
                     /locus_tag="PLJ75_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04043.1"
                     /translation="MDLLRIWQEADMKDIQQHLLMADDLFGFCPGCKTPGLKLQDLHT
                     CPTCNREFKYVTSKDARGGKSAEVVMRLKKKLPHLIFVDYDDYERLSSKNKAADLFKN
                     M"
     gene            complement(49010..50722)
                     /locus_tag="PLJ75_02105"
     CDS             complement(49010..50722)
                     /locus_tag="PLJ75_02105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD04044.1"
                     /translation="MKKITAHASITWFSSFFNKENIILLLILFFGLLFRFLTITNIET
                     GGDAASYWFAAKRLYYGLPYILNHHTVRFGMILPVTLSQCIFGTHPIVYYIMPLLFYI
                     LQIVFLYKIAVRACGINIAFLSSILLILYPKMFSHAVQLKPDGFLAAYILISAWFIVK
                     FHDSSKHQYWYLLVSAFFMFMAYLTKETALFFIPGFAICIWMMKRKINHVLFFGFVLF
                     VLFLCETALYYYLAGMPLGRAQIVAGSHLSSGNLMALPSVWSLLSRYAQLNVFEKLYF
                     YAYILSTCYLIIRMKKLKPDVTIQSLIIIPLVFFILLTFAVKSIVPVVPAMSFNPRHL
                     VPAAPFMCFILAYAIVTVKNSLRMSSTTSHHISPAAQSSVAMYAWITVVLSVVSTIAT
                     IIVLPHFPQAARSNFLREHPFVETFRYYQLLNDAYAHGIPIIQEKVVADRWKKPVEAV
                     MFFLNQGYSLQEACQKAKVTEKDYLYCYKRVQQGDYKTFKIFTHIFWDANFKLGSDIT
                     FPKMEEASIKAKSIGFIVNEECKKNINYTSQFFNNEENVVVVMQEKPLRVKKMKLKDF
                     LTTE"
     gene            51079..53163
                     /locus_tag="PLJ75_02110"
     CDS             51079..53163
                     /locus_tag="PLJ75_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HPD04045.1"
                     /translation="MAKKDTSDWKFIVVNTLFWLLLGGLLFSNKSRFTIGGAILFLII
                     GSLTIYLGLKKSGGLRYPGLVRIMAVVSGIIYILTSGWLFIDYFTSDEHISMKGRIAQ
                     VANKETTTLDLSGRKLTALDPKIMELSTLTFLNLENNRLTELPPEIGRLVNLTFLNLE
                     DNQLTELPPEIGRLVNLTFLNLDNNQLTELPPEIGNLTNLKILWLRNNHITNLPPEIG
                     NLHKLESLALSDNQLSTLPSEIGQLSSLQHLALYRNQLTVLPREIGQLSNLTTLFLDD
                     NQLTKLPPELERLTQLQTLSLNRNPLGELPDRFMEQAQSRGLKISYKPDEKLPPQGDG
                     GLLLFLVVFVAIILITFGLNRWIGKREEQLKLNLNTSGRVYAIPAGGRAGCIFVLLLK
                     IMVDVFLFIASLHPEETGVAPAAGIGIPLLFSPLIIICLYILVHYDGFIVLTGDAIIL
                     RRLWGEKRLPYHEITDVKESSLWLPFNMIIKGTHTKLSIPRSIEKFPELYGALMQQIT
                     RKPSVPDITRKFDAPSFPYHLALTRKARIFSIGGSCLLVILYLGIGLAGFWVPYIQAK
                     PPAYDFAFFLFGAISLFFLPAIFIFVKGMIAAKQPIEMILTAQSIGFRFPGKSWQTWP
                     IEQISAIRLEPVKINISVKNSPVFLWSEATAYRIILKSNTQQLMIEQDRARQFGFQPE
                     QLYRVFQQLYHG"
     gene            53712..54638
                     /locus_tag="PLJ75_02115"
     CDS             53712..54638
                     /locus_tag="PLJ75_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD04046.1"
                     /translation="MKNHFQSFSDKYSEKYDTEYGKIRLERIDSFKDSLISCGDYTKG
                     IARIQCSNPECKHEIFRPFSCKRFYFCPSCSQKRAILFGEHISNEVLFKLPHRHFVFA
                     FPKMLRLFFRNNKKVLSDIARLICEMIIEYYKMLTGKEIRTGIVLAFQSYGDFLRYNP
                     HFHALIMEGGLAKNLLSWKHSGFSIDNSIRVATSDNKAREAIAQYMARCPISLNKIIY
                     EPFKGKVIFKTKYNKYFKENHKVYDADEFIAKLVQHIPLPRIRLIRYFDLYSSKSRGK
                     WKDWDHVSKLAPDGWKEQNGIGIQGEEIEKTG"
     gene            55225..55872
                     /locus_tag="PLJ75_02120"
     CDS             55225..55872
                     /locus_tag="PLJ75_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04047.1"
                     /translation="MKKNRTALGVIASICLIVPVLSGCGPKIITVSLRDNIKFAERTP
                     KIALPIGLYVSPEAREYVIKYQLDGPLGKVNEFRHMPIGAALMPNGLRSLSKVFQTVQ
                     PMDSPANAPAYYVELKVDPASSLEMGRWKFSEKSVDLHLVCMVKRGNGALIWKKTMNS
                     KAAKSNPQGWLAGVWMPAAIKSSNTKLKAAGEESLYLCLEALNAELIKNRAAIFR"
     gene            56299..56952
                     /locus_tag="PLJ75_02125"
     CDS             56299..56952
                     /locus_tag="PLJ75_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04048.1"
                     /translation="MIIKGTHTKLSIPRSIEKFPELYGALMQQITRKPSVPDITRKFD
                     APSFPYHLALTRKARIFSIGGSCLLVILYLGIGLAGFWVPYIQAKPPAYDFAFFLFGA
                     ISLFFLPAIFIFVKGMIAAKQPIEMILTAQSIGFRFPGKSWQTWPIEQISAIRLEPVK
                     INISVKNSPVFLWSEATAYRIILKSNTQQLMIEQDRARQFGFQPEQLYRVFQQLYHG"
     gene            57252..>57476
                     /locus_tag="PLJ75_02130"
     CDS             57252..>57476
                     /locus_tag="PLJ75_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPD04049.1"
                     /translation="MIKIGTRGSDLALWQAQFVAGKLACDYEIVTIKTKGDRVHNVSF
                     DKMEGKGFFTKELEDALLEGRIDMAVHSMKD"
BASE COUNT        17617 a        10157 c        11927 g        17775 t
ORIGIN      
        1 tcctcgcacg agccgtagag cgctacggca tccgtgcctt cgctgttttt tcgttcttat
       61 acagtaataa gtcattatca gcataaaata tgctgttaat tcttcataac agtttttgtg
      121 gtttcattcc gtagagatga aaaaaatggg gtcagagccc tggtactttt cttcgtttgt
      181 tcaaaaaaag gggggaggta cacgattctt tggcagcggc tcaggatgac cgggaaaaaa
      241 taaaaaattt aaaaaacagg ttgacaatca cttaaaatgt aatacaataa tactataata
      301 cattaaatat aaacataata atatttatga ccatacaatt tatgtcataa ctttaaggag
      361 gtttcaatgg caaaaggtga cagagttgca attattgatg ggtgccgtac ccctttttta
      421 cgttccggaa cagcatacag ggagatgatg gcatatgcta tctgtcgtca tgctgtgaag
      481 ggtttggtgg caaaaaccgg tatccccaat gatatggttg accatggtat catgggatcg
      541 gtgctcaccg atgttaaaac aaccaatgtt gctcgtgaga ttatgctggc ggctgcactg
      601 ccaaatacca tccccgccta tacgtgtact gtggcatgta tatctgcaaa tgctgctatt
      661 ataagtgctg ccaacctgat cgttaatggg aatgcggatt gtatcattgc aggcggtgtt
      721 gaaacatttt cggatccgga tataaaaatt tcaaagaact atcgccaatt tattatggac
      781 ttaaccatgt ttaaacgtcc cagaactatt gtgggaaagc ttaaactgtt aaagggcatg
      841 agctttaaag attttatcgt tcccgaacaa cctgaaatag gtgagttttc cactcaactg
      901 cgtatgggtg atactgctga taggctggca aaaaggctgg ggatatcaca gcgcgagcag
      961 gatgcatttg ctgcgctgtc gcataaccga gctgcagcag catgggaaca agggatatta
     1021 aaaaaagagg ttgtgccggt tgtaaccccg cagggcagac tggtagacaa agataatggt
     1081 ttcagaaaag ggacaagagc tgaaaaatta gctgcactca agcctgcatt tgataagcgc
     1141 tacggtacag taactgccgg caactcttca tttttaaccg atggcggcgc tgcagtgctt
     1201 ttgatgagtg aaacgctggc taaaaaaatg agattaaaac cgcttgccta tatcaaatcg
     1261 tatgccttta cggctcagga tttatgggaa gagctgttac ttggtcctgt cttttccata
     1321 ccaaaagcac tggcaatggg taaattaaaa tttaaagaca ttggagtgtg ggaaattcat
     1381 gaggcatttg gcgttcagat ggttgctgta gtgcaatgcc ttgcctctga ctcgttttgc
     1441 aaaaacaggc tgggattgtc cggtaaagta ggtgagatta atcatgatga ccttaacatc
     1501 tatggcggtt cgctatcgct ggggcatcca tttggtgcaa caggtgcacg ccttgtaaca
     1561 acatgtgcca atcgtttgca ggaaagtggc aagcgttacg gtgttgttgc cgggtgtgct
     1621 gctggtgcga ttggcaacgc aattatatta gaacgatcat aaggaggact gcaatgaacg
     1681 cattcaccta ttgtaaacct gaggttttga acgatgtgct ggtagtaaca atagattgtc
     1741 ccggcaaagt taataaagtt tccagtgcta tgctcaatga aatggaagta attattaatg
     1801 atgtaaagag caaaaaatat aaaggtttgg cgatagttag cgggaaggat gacaattttg
     1861 tggtaggggc tgatcttgat gaacttacat ccatgaaaac tgaagatgag attagagcgt
     1921 atattacaaa agcaaatagt gttttgtccg gattagagaa acttcccttc ccaacagttt
     1981 gtgttatcaa tggcaactgt ctgggtggcg gtgtggagct ggcactggtt accgattacc
     2041 gcattgcaac tgattccaca aacaccgtga taggatttcc tgaaattaaa ttaggactta
     2101 ttcctgctgc gggaggaaca caacgtcttc cacgattaat cgggattcaa catgcacttc
     2161 ccctcattgt gcagggatca actatacggg caaggcgtgc aaaacggctt gggattattg
     2221 atgagatagt tatgccgtat ggattaaaag aaatagcagt gaaaaaggta ttacagctaa
     2281 aaggtaaacg aacaataaaa cgaaaaagaa catttgctga gtggctttta gaatcaaatc
     2341 cgttagggcg cagtatcata ttttcaaagg cacgcaccat ggtgatgagg caaacacggg
     2401 ggttatatcc ggctgctgtt gctgtcattg acgctgttga atatggttat aaaacaacta
     2461 tcgcaaaagg cattgcaaag gacagtgagc gttttattca tcttgttcaa agttatcaat
     2521 cacaatcact tgtaaacctc ttttttgcca tgaccgattt aaagaaaaat ccgtttaaaa
     2581 aaatggaaaa aaatgtgtca aagctggccg tcattggaac ggggctcatg ggtagcggta
     2641 ttgctttcgt ttcattgccg ctgtgcgata cggtgctgtt aaaagacacc tcgcttgatt
     2701 ttgttacacg cagcatggca cagattagaa aaagcctgga aaagcgggcg cagtcggggg
     2761 ctatccgtaa ttttgacaag gaggtaatgt acggaaaact tattccatgc gatgattata
     2821 cctattttaa aaatactgat ttggtcattg aagccgtttt tgaagatttg caattaaagc
     2881 aaaatctggt aaaacaggtt gaggctgttt gcggcgatac cactattgtt gcatcaaata
     2941 cctcggcaat tcctatcagc cttattgcga aagcatcaaa gcgtccgcag aatatgatag
     3001 ggatgcatta cttttcaccc gtacctgcca tgccattgtt agaaataata aaaaccgcaa
     3061 aaacagcaga ttgggtggtg gcgacagctg ttgcaaccgg gatagctcag ggaaaaagtt
     3121 gtattgtggt aaaggatggt cctggttttt atacaaccag aatattagca ccgtatctga
     3181 atgagagtgt tgttttgatt gaagaaggtg taccaatgaa tgatattgat agcgctatgc
     3241 ttgcgtttgg ctatccgatt ggtcccatag cactgctgga cgaggttggt atagatgttg
     3301 gtgcccatgt taccattgaa ttaggacaga tgtttaccgc gcggggtgct gcaccgtcca
     3361 atgttctgcc gttgctttta agcaaaggct ttaagggcaa aaaaaataaa aaaggatttt
     3421 atatgtacga tagtcctaag aaaaaaggca tgcgtttgcc caataaagaa gtgtacacgg
     3481 tcatagggaa agtaactcca gaagcaatat cagtagaaga gattcaacag cgggtaagcc
     3541 ttatgatgat aaatgaagca gctcactgct tgcaggaagg tataatctcc tcaccgcgtg
     3601 acggcgatgt tggttccgtt ctgggattgg gttttccacc atttaagggc ggaatattta
     3661 gatatattga ccatgaaggt gccggtacca ttgttgatac catgttgcaa tactacaaca
     3721 ggggtctgga gcgttttaaa cctgcaatga tactggcaga tatggcaaaa acggataagc
     3781 gattttataa ggattaaata aatggaacgt tcaattcagt ttacgcagga gcatgctctg
     3841 ttcaggcaaa ccgtacgcaa gttttttgag caacaggtaa tgccttttta cgatacatgg
     3901 gaacatgaag gcattgtgcc tcgtgagatg tggaatcgct gcggtcagct tggtttgctt
     3961 cttccatggc ttgaggaaca atatggcggt ccaggtggcg acttcctcta ttcggttatt
     4021 ctcaatgagg agctggcata cagcggtgct aattcattta tgattccact ccacaatgat
     4081 attgttgcgc catatatata ctcatttggc acagaagaac aaaagatgaa atacctgcca
     4141 aaatgcgctt caggggagat agtgcttgcc attgcaatga ccgaaccgga ggcgggttcg
     4201 gatcttgcag gcatgaaaac cagtgctgtt tccaatggcg atagctgggt gctcaatggt
     4261 cagaaaacat ttatatcaaa tggtatcctg gcagatctgg ttatagttgc agccaggtcg
     4321 tcaaaagagt tacctcccca tcagtcggta agcctttttt tagttgaaga tgggactgaa
     4381 ggctttacca gagggaaacc catcaaaaag attggtctta aagcgcagga tacagccgaa
     4441 ctattttttg ataactgtcg catccctgac aataatcttt taggacagga aggacaggga
     4501 tttatttatc ttatgcagaa gctgcaacag gaaaggctgg tatgtgcgtt aacttcacaa
     4561 gcagccatgg aaaaatgttt ggcaataact atcgattatg taaaagagcg aaagttattt
     4621 gggaaaccac tttcaaaatt tcagaatacg cagtttacca ttgccgaaat ggcttcggaa
     4681 attgcagctg gcagaagctt tattgatgat ctgatacatc accatataca gggtcatgat
     4741 gtagtgaaag aaacctgtat ggctaaatac tggatatgtg aaactgccaa acgtgttgct
     4801 gaccgttgct tgcagctttt tggagggtat gggtattgtc tggaataccc ggtatcacga
     4861 ttttatgtgg atgctcgtat acaaactatt tatgctggta catctgaagt tatgaagatg
     4921 attgtatcca gaatgatggg gttgtgattt attagttgac aaatcccttt gtagacgatg
     4981 taatccatgc gttttcacaa atatgttcat aaatcaaaat atgttgattt gcggaggttt
     5041 atatgaatac tgtgatacgc aagggttaca cctatgcaac gtggttattc attggcgttg
     5101 ataccgcatt ctggggaacc atctcgcttt tttcatggtt tgtcagcccc agaggtgacc
     5161 ttgctcattt ttgcatgcaa aactggtcaa agaatatact atggttttgt gggatacgat
     5221 atcaggtgga gggtattgaa aatatttcaa aagaacatgt tcagatattt gcatccaatc
     5281 atcaaagttt ttttgatata tggcttttga gtgccattat tccggtaaag tttggctgga
     5341 cggcaaaaaa agaactattc aaaataccat tcatgggatg gcacatgtct ttaagcggtt
     5401 atatagccat agatagaaaa aataaagagc ggtcaagagc cagcatgaaa gaggcagcag
     5461 taaaaatacg aaagggaaac cgtattatga tatttcctga gggtacgcgc agccgtacgg
     5521 gagtgctcca accctttaaa aaagggttat tttatttatg cattgagaca ggcgtgccga
     5581 tagttcctat ttatatccat ggcacctatc aacgattaaa gcccgaaacc ggggatatta
     5641 accctggaaa agtatatgtt atgatagggg aggagatgcc aacagacgaa tatactatta
     5701 ataccatcga gaaggcaatg aatgatttac gggatagaat tttaacttta caaaatgagt
     5761 gcctgaagcg atatggtgaa gcagtgtaag attttgaatg caatttatag atagtatgga
     5821 gattttcaaa tatggaagac ttaaaaaaag catatcgaac catcatggat gaccattttc
     5881 cggataccat gaccattagc tttgggaatc aaacgctgat atataaaaaa cgaacctgga
     5941 agatcaatga tgatgggcag ttgattgaaa agggattgcg gtatggtgaa aatcccgggc
     6001 aggaagcagc actctatgag cttgttaatg gcaatcttgt gctgggggat tgtgaattca
     6061 taagccccgg tcattcgctg gtaagtggca ttgatgaatc gatgatgctg cagtttggga
     6121 agcatcccgg caagattaac cttaccgata ttgataacgc tctgaatata gtaaaatatt
     6181 taatgaagca accagctgct gttatcgtta agcacaataa tccctgtggt gttgcttatg
     6241 gagtaactat cgcccaggca tataacaggg caaacagggc tgaccgtatt gcagcttttg
     6301 gtggatgtgt tgtctgcaac agagctgtag ataaagaaac tgcacagctt atttcacaaa
     6361 attatctgga ggtgctggtt gctccggatt ttgagcccgg agtcatggat atattgtcaa
     6421 agaagaagga ccttcgcatt gtaaggattc agcgtattga caggcttgaa gagtataagc
     6481 aattgcgctt tgttgacttt aaatcgctga ttgatggcgg catcatagtt cagcaatcac
     6541 cggttactat cattaattct aaagatgatt taaaacctgc acaggcagaa tataaggggc
     6601 agctttataa aattgaccgt ttgccaaccc cccgggaata tgaagacctt ttgtttggct
     6661 ggtttgtgga gcagggtgtt acctcaaatt cggtactgta tgttaaggat ggggtaaccg
     6721 ttggcatagg taccggcgag caggataggg taggcgtagc cgaaattgcc gtgcagaaag
     6781 cctataccaa atatgccgat atgctatgtt ttgataacta tggaatttcg tactatcagt
     6841 tagcacttga ggttgaaaaa ggattgcgta aagcaagcga taaaatagca attgatgaag
     6901 ctacaaaaga agcaaagggc ggactcattg gagcaagcat ggtttcggat gcattcttcc
     6961 cattcaggga tggtgtagac gtgggtataa agcagggtat ctctgccatt gtgcagcccg
     7021 gcggttcaat ccgcgattgg gagagtataa tggcatgcaa tgaagctaat ccaaaagtgg
     7081 ctatggtttt taccgggcag cgtgcgttta aacattaaag gcaaaggcgt tcaatgacgc
     7141 ttttgctcat attcctttat atattcttct ataaggcttc ctgttttatg tggctcttct
     7201 tttaccggtt tcatgcgccg ggctgcaaca ttacctatgg ctttaacggg tttcttgaac
     7261 agtccaacaa taatatctat accatcacga attatagtga acaatttttt tacaatagat
     7321 gttctggtgc cttttggagt agtacgcata ggagtaatgc ttgaggcaac tgtctctgct
     7381 acatttttta caccatacat tgctggaatt ggattgtagc tatccttaac atacgctttt
     7441 tgtggtctaa aggggacctg ttttggcaat ctgcttgtta gtaatatgtt tgtccctgat
     7501 tcggttgact tatcaataat aaacaggaat atataatata tatagagaaa taaatttcca
     7561 aagatggcaa aaaagtgata ccatatatcc atgaatgccc agaagacatc aacccagatt
     7621 tttaaaaaat ctacaatctt atagaagaag ttgaaaaaat tatcaacggt attaatccag
     7681 aattgcttta caacacgata gttcataaca gtaacctcag caatactaat aaatgaagac
     7741 tataaacata tagtaaattc cctgtaaaaa gagtcaatta aattattgca attgcaggct
     7801 gctatattat ttgtactatt ttgtttatag cttgttttcg atagtttttt aaattaattg
     7861 tattttataa gtgtatttta tttaaaaaat cttgataata gtgttgtata ttatatactt
     7921 gacactatgg aatatttaac agctatacgg ccatatataa ttaaaaggtt agaacaaata
     7981 aaaaaagcgg atgtacttat tggcattccc tgttttaaca atgaagcaac gatagccaat
     8041 gttatgacaa tggtaagcga aggtctgttt acatattata atgatgtaag gccggtactg
     8101 tttgtttctg atggtggttc tactgatgat acacgcgata tcgctcaatc aatagaactt
     8161 aagccatggc aggaacggat aatacagatt tatcgtgggg ttgccgggaa aggttcagca
     8221 ttcagagcaa tttttgaggc ggcacagata cttgatgtag atgcgtgcat agttgttgat
     8281 tcagatttac gcagtatatc tcctgattgg attaaactgt tgttatcgcc agtacttgaa
     8341 aaagactttg agtacgttgc accggtgtat acacggtata aatatgatgg aactatcacc
     8401 aacaacattg tctacaacat tattcgtgca gtgtttggga aaaggatacg acagccaatt
     8461 ggtggtgact ttgcaatctc aaagaatctt gcaaaaattt atttagacaa gccggtctgg
     8521 acaaccgata tagcccgttt tggtattgat atattcatga ctgtcaatgc tattgtgagt
     8581 catgtgaaga tatgtcaggc aaatcttggt gtaaaaattc atgatgcaaa agatcctgcg
     8641 caatcacttg gtcctatgtt tgttcaggtt atcggaacgc ttttcaatct tatggaaggg
     8701 tatgaaccgt actggaaaaa tattgaacgc agtaaacctg tgccaacctt tggcgatatc
     8761 aatgctagtg agcctgaacc aatagaagtt aatttagaaa ggctggtgaa tgagtacaag
     8821 ttagggtttt cacaatttca ttcattgtat caagagatgt tttcaagtga tgtgtataat
     8881 gcgctgttgt cttcatcaaa gcttgatgtt atacattttt caatacctgt tgaagtctgg
     8941 gttaaggttg tgtatgaact ttctgctatt tatcatacat ggaagataaa taaagtacgt
     9001 ttaataaacc ttatggtgcc attatatttt ggcaggattg cttcatttat caatgaaaca
     9061 ttgtcaatga attcgtttca ggctgaggaa gaggtggaaa aacaggcgca ggtttttgaa
     9121 aactataaac cgtatttaat agaaaagtgg cagttacagg gtaaggatat accatttgcg
     9181 taatagcata ttatactttt ttaaaaaatt gtatttcacg ttgtaataat gatatcctat
     9241 cttttaaatt tatagaattt gcggccagct cttctgaacg agcagcaatt ccctgggttg
     9301 attcattaat gatactgaca gattttatta cattgtctga tgcagtttgc tgttccctta
     9361 cggaattatt gatgattgaa gaaatatctt taactttagt catctcttca acaaactgat
     9421 tcttagtgtt attttgtatt tccattttat ttgatatttc gtacatcatt gtaccaatca
     9481 ttgaaaatcc ttcaataatg ctttgaattg ttgaaaccgt attctgtata ttgacaatac
     9541 cattttttat ttcgttatcg ctggataaaa tcaatttttc tatatctttt aaacttgtag
     9601 cggtttgctc tgctaatttt ccaacctctg atgcaacaac agcaaaccct ttgccagcct
     9661 caccggcacg ggctgcttca atggctgcat taagtgccag tagattaata tgatcagaaa
     9721 tagtgtcaat aattgctata atatcaatta ttttttgcga gcttttctgt atggaggtca
     9781 tagattcatg catggtttgg agcatatcct gaccttcttt tgcttttgtc tgaaatttat
     9841 tgctgttttc ctttgcatca attataattt tatttaaatc attcattatt tcagttaatg
     9901 aatcaaacgt agctatcatg gttgagatac ttacatcttg ctgattaaca cttttgctga
     9961 tttcttcaaa tcctgccccc aattgttcca tggttgccat tatttcttct gaagaagagg
    10021 ctaacgtatt gctgttttca gaaaaatctt gtgttgattg agaaacttca tctgatgctg
    10081 taaaggtttc ctgaattatt tgacttacat cttctataat atttcgcaat ttatccatga
    10141 atgcattaaa ccatgcaccc agtaggtcta tttcatttgc tgcattactt tggtatattg
    10201 agcattgcaa gcatgatttg aattgtcctt ccaatataaa tttgcaatgt atgtcgttac
    10261 caaacttttt agcaatactg cctatttcaa aaaaacaatg ttttgaagat gtatagggga
    10321 taaaggtttc accttgtttc ataaaaattt caatattgtt tttattgaat gttactggat
    10381 attcagatga aaggtttcca acacttgctt cgccaaaact tttaataaag agtgatagtt
    10441 tatttttaat atttttggaa aaggtaatgc taaaaaatat ggctattgct gcaaaagcta
    10501 tgctaaccat aacatataca attattgtat ggtatatagg ctttagcaat tcagcataag
    10561 aaatttcaat caaaaacatg tactgaaaat atgaaagcca tttccataat cctatgacct
    10621 tatcattgta tgcattatca tactgtgcaa tgccagatct aaaatcagaa aggcattttt
    10681 ttacactggc aattagctgc ccatctttat tgacaggctt aaaattttta ctgtgcctat
    10741 catcctgcaa agaataatcg ttaaaatatg aattttttgg tttattgtta tttgctgcca
    10801 ggattacttg tgcatcctta tcaatgagat aaacattgcc atggctaccc acatttatca
    10861 tcctcattga atcaaatagt atatccatat tgacaaaaca aattatataa ggtgattttg
    10921 ttttaacata atgtatagat ttgccaatag gacaactaat aggcaatgca gttatactat
    10981 tacctttgaa cggtattttt attgttttac aaattgcagt atcattggta ctttttattt
    11041 gattgtagat agcactcatg gtaaatgaaa atgtgctatt ggtggtacac agtacatccc
    11101 catcgtcatt cataatggta atggcgtagt attcaccctg atcagcaata acctgatgca
    11161 aatattcaat gagtttattt atttgtttgg attctatata ttcattaatg atagatgccg
    11221 ttgataattt ttcaatatgc tctactttgt tttccaacca atattcaata aattcaaatt
    11281 gttttagcaa tatttcatgg taattattta ccatgctttg ttccatctga ttagctgtat
    11341 aaaagcatgt aattaacaaa aaagataacg atatcaatat aacggtaagt ataagcgcat
    11401 atctgataga ttttgttata cttttatcct tattaaccag ttgttttaaa tattctgata
    11461 tcttcatata ccatttcaat taataataaa ttttgttcaa tttgttctct ataaaacttt
    11521 ttcataatga cagcgtaaac atcccaatgg acctttgctt cttctatggc attcatgaca
    11581 ttgttgatga aatgctgaac gcaaattgat tatttccttg ttatcctttt ttgtatgaca
    11641 ggatgagcat ttttgtaatg aacctatatg atgacagtca atgcattcgg tgttatacga
    11701 ttctatatgg gctttgtggt taaattttat aggtggtaat ctatgccccg ttttttcaag
    11761 tataatagtt tcatatttta taatgttgtt ggtttgagtt cccttatggc attgttcaca
    11821 gctaattgaa ataccttgtt tcttatgaca atctttgcaa aaattatgca aaacaaccat
    11881 ttttgaatgt cctgcatggc aattactaca tgattccggt tttgtgttgt tttttgtatt
    11941 atgatgacat tgtatacatt ggatgttttc tttactatgg atagaatgat ggttctcagt
    12001 gaacgcaaag gtttttttat atattttctt aatttcatta tattcattca gcctgctgct
    12061 tttttcttta tggcattgat tacaggttac aggaccttta tgctttactg tatgacagtt
    12121 aatacaaaag gtatggtaca tctctgcacc catggtaccc ttgtggcaaa aggagcatgt
    12181 cttaatacgt tcgtcattat attgtttatg gtggcatgtt ttgcattgta ttgatgcgct
    12241 ggaatgtgtt tcatgagata tcacaactgc tggaagggtt gatgcagtag cggttattcc
    12301 ccttgccctg tcatcgaatg taataaaatg atttactttt gttatttcta taacatcggt
    12361 tgctgaagga ttaccagtac atgatatgag tatataacaa aaaaagaata taaaaaatat
    12421 taatgagcaa caacaaacac gaaataacaa tcgtatgttc atcccttcat ttccttcacg
    12481 ctataacatt acaaaatctt ctatatttct ctctattgct caatagtttt gtatgttaac
    12541 acaatagaca atctttgcat tcattgtttt aaaaccaaat atcaaagaac gtattaaaca
    12601 ttaatctata tactattaat cgtcggtagt tgtcaagaag taaattaata taattaaaaa
    12661 aatgttgcat atatagttgc tatcgtaatc aaatttgtgt atacatattt tttactcctt
    12721 caataactcc cacaacacaa tgccagccgc caccgatgcg ttgagtgatg aaacctttcc
    12781 tcgtaatggt attgcaaccg ttatatcgca cagttgttgt gtaagctggc gcatgccgct
    12841 cccttcactg ccaataataa gtaccgccgg cttatactgc cgtatgctat ggatgggatg
    12901 cgtaccgctg tcggatgtgc caataatcca gaagccagat tgttttgcat cgtttataaa
    12961 gcttgctaaa ttggcaacat aatgaacaat aatatgagca gttgcacctg ctgatgcttt
    13021 tatgatggtt tgcgttattt caggggcatg ggcttttgtt acaatgacag cattgccacc
    13081 cagcgcttcg gtagtgcgta ttatggagcc aatattatgg ggatcggtaa tgccatcgca
    13141 ggcaacaata accccctgtg aactggcaat atcgggcaaa tgaaatctat tctgtagtgt
    13201 attgtgtggg cgatagctca tcttaagagc tactccctga tgatgaggct ggcagtggct
    13261 atcaaaccag cttctgggaa ccctgttaat ggggatattc agactggaag ctttatctgc
    13321 aataacatca agtatcctgc catgtgcact ttcggaaatg tagagggttg catcaccttc
    13381 aattgatgat agatattcaa ggacaacctt gcgtcctgct atgatttgat cagccaccgc
    13441 gtcccctctc tggtatcctg tactataatc cctttgctat acagttcatc acggatacta
    13501 tcggcttgtg taaaattttt ttctttacgg gctttattcc gctcttcaat aagagcttca
    13561 atttcttctt tgtttactgt tacagcgcca tggaagaata taaatccaaa aacggtgtca
    13621 atatggttaa gagtatgcat tatagtatcg gcgtcagctt ttcccagcat acgtttgtct
    13681 atcaatgtat tgatatcatg taaaaagtca aaaaatgtac caatcccttc tgcaatatta
    13741 aggtcatcat caacagcttc aataaatgat tctataaact gtgagcatcg tttatctgct
    13801 tcctcattat caggcttttc ctgttttata tcgttaaggc gggcaatgag gttgtcaatt
    13861 ctaaagagtg cctgcccggc ctgagttaaa ccgtcaaagg taaaattaag ctgttttctg
    13921 taatgggcgg taatcaatag atatctgatg gcacgtggcg aatagccttt attaaggagg
    13981 tcacgcaggg taaagaagtt ccccgatgat tttgacatct ttgaaccttc aacaagcagg
    14041 tgctcaacat ggagccagta acgtacaaaa ggctcatcat acgctgcttc gctctgggca
    14101 atttcatttt catgatgagg aaatattaaa tctacaccac cggtatgtat gtcaatggtg
    14161 gtgccaaaaa ttttgcgcac catagccgag cattcaatat gccacccagg tctgccttta
    14221 ccaaaagggg tttcccagta tagctcattt tctttaggag ctttccacag tgcaaaatca
    14281 cgaacatcct ccttggtata ttcatcggca tcgtatcgcg cacctgcttt gatatccata
    14341 ctggtgacat tgctcaactg tccatattta tgaaactttg caatgctgaa ataaagtgag
    14401 ccatcctttt cataaacaag ccccttttta tcaagatgtg ctataatatc tatcatggcg
    14461 tcaatagatt ctgtggcacg ggggtaatgt tcaacaggtt ctatgtttaa cgttttacaa
    14521 tcctcaaaaa atatccgtgt atacttgtcg gtgtattctt ttaatgtcac cccttcttta
    14581 agagctccgg ctattgtctt atcatcaaca tcagtaatat tcattgcatg gtttactgta
    14641 tatcctctga atttaagata gcgccgcaaa aaatcattaa atataaatgt acggaaattg
    14701 ccaatgtgag cataactgta caccgttggc ccgcaggaat atatggtaac agggggatat
    14761 tctgcaatac tatcttttgc aatgccaccg gggatgaatg gctctaaacg tcgtgttaat
    14821 gtattaaata ttttcagcgc caatatatag cctccattat tctgcacagt atgtatacat
    14881 actgttgtgc acagtatgct ctaattattt tgttttctaa aaaagatgtc atatagtttc
    14941 aattctaaaa acatagattt aatttttcca aaaaatagca aagttggatt aatttttatg
    15001 ggtaatccca gccttttata cctatacata gtgttcatgc cgggtagttt ggtaatctgt
    15061 ttttgcgtta cagtgtgcgt cattggtaaa tgtttacaat agttttaaca ttcattttcc
    15121 attgacaggc atgccgtcga tagcctttca attgctatag tatgtcaact atagctggta
    15181 aagatagctg tcaataacta aattaatcct gcattatctg ttcttctacg gggacgtact
    15241 caacaacttt ttcttttatt tcaatagaag gctgcggggc tggtatttca gagggttttg
    15301 cataagcttt ggtaaaatca atcttattaa aatcttccgg cgaaagttta tagtatgcta
    15361 atataagatg gagtttttta atatattcaa taagttcttt aagttttttg ttcttgcggt
    15421 caatggtttc acgtgattcc ttaatgatac gttttaacaa atcagttctt ttttttaacg
    15481 tatcttccag ttcaacaaca cgttcataat actctttgtt gactttttta aagtcaatac
    15541 gggcaataac aatttcacct tcctcttttt ctttttctat ctctttaata atatcattaa
    15601 gttcttttga agacaaaaga tcttttgtcg atgtctgcag ccattttttg ccatcgtcat
    15661 cggtaaactc ataaacatca ggcaatactt tctttttacg tttaaataac ataactattt
    15721 tgtgagatta ttatttatta tactatcgac tatttataca ttttatttaa tcaaatcact
    15781 ggtttgtata atctttcatt tttgcattta actctttatt agacgtattc cacataatgg
    15841 tattggaagc ccaatgtttg tattctgtga aataggttgt tgcaatacca attgcctgat
    15901 aaaatggcag ttgcatagta tacccttcat catcggtaat ttcaatgtaa ttatcaggaa
    15961 caccaagcgc aatcgcatta agtgataacc gttgtaaaag ttctaaatca aaattgcttg
    16021 cagctaatac attaaaaggt tttttagatt ccagctttac aacatagcta ttttttttaa
    16081 tgaaatacat gcaattatta tccgtatcca tgacagttgt atcaaaattc cttaacacaa
    16141 tgagttgtcc tttgttaacg tatgatttta ctacctgcat aatgatgcgc agtttaaccg
    16201 agtttatata cagtggctgt aataactcta aatgtttttt gatagtttct ttttgctcat
    16261 catctgctgg cttttcttca ggatttttat agagcgatag taccttttga aggtccagat
    16321 tttctttgtt ttcagcctgt atacaggcgg atacgggctg tgaagtgtca atcacgacac
    16381 tatcgtaaag agcctggacg cgataccagt aaacaattcc cggtacaaca tcgttatcag
    16441 taaatgatgg agttgttatt gtggcaatag tatcatatgg tccttcacga aaaagagaac
    16501 gaacaaatgc aaacgaagta ttgtcgccat aggtttcaca atgtatggtt atctgattgg
    16561 tggtgctgtc aggtagcgta gagacattgg ttattttaat gatttttgac tgaaaaatca
    16621 atgataattt tgtttcgata gttttaacaa tatcagcaat ggcatggtaa taagcccagt
    16681 caaatgatgt tgcaataaat gatccttccg tgttagaata taactttgat tcaataaaga
    16741 tctgcccaac tgaatgcgac atttcaaaaa aaagtatgta atcatttttt ctttttctgg
    16801 ctgtcttttt tattacggaa agcgaatcct cgttacttaa ttttatgaat gaatctattg
    16861 aacctatttg ctttgtgttc tctattgaat agttttcaat agtcatttta ttcaataatt
    16921 caaaaagtat ggtagcttca ttttttaagt catcagggca aaaagggtaa acaactaaaa
    16981 gtgttttagc ctgtacaacg cttgttgcca ttgccatcaa taaacatgat aagaaaaatg
    17041 cccttcttac tttcttcata taaataccta attttttata attgctattt gctgatagca
    17101 ttgtaaaata tttgacaatt ggttatgcaa ctatcatact caatatactc aatggtaatg
    17161 cctgtatgca agtaaatttt atggcatgtt gtataaaaat agtgatgagc tcatcaatca
    17221 tttttcaggg aattaccaat ggttgatgtt tagtcactaa gaatggatga aaagtataca
    17281 tacctcaccc ttattccctc tccttaaata aagagaggaa aacaaggccg gggctgaggc
    17341 taataattct attttggagg atttgccatg cctaaaaaac ataaacagcg tgacgatata
    17401 aaagccatat atacccatgc tattgcagca gttgatccct atagtgccgt ttgcaaattt
    17461 ttacaacgga agggaaatac attaattatt aagggcgata gtaacctggt cattgattgt
    17521 gataccattg atgcaatcta tgttgtaggt gctggtaaag caacagcaag gatggcaaag
    17581 gctgttgaag atatcatagg agagcgtatt gttgagggta ttgttgttgt aaaatatgga
    17641 taccgtgaag atttgcagta cattcagcag cttgaagcgg ggcaccctgt acctgatgag
    17701 aatggactta aggcatcgca aaagattata gatgtactgg gaaaagcagg tgagcatgat
    17761 cttattattt catgtatttc aggaggaggg tcggcacttc tgccctatcc agtagaacca
    17821 attacattag cccagaaaca ggatttaacg caaaaacttt tgaagagcgg agcatccatt
    17881 aaagaattga atgtggtacg caaacatgta tcgcgcacaa aaggcggcaa cctggcactg
    17941 gcggcttacc cggcaacggt aatcaatatt atgatatcgg atgtggttgg tgatgatatg
    18001 gatatcatcg catcgggtcc ttttgtttgc gataccagca cgtatgctga tgcactcgct
    18061 attttagaaa aatataaatt aacaggaact atcgcccatg ccattataga gcaccttgaa
    18121 aaaggggcac atggtgaaat tcccgagaca cccaaggatg aaaatatatt taaaaatgtt
    18181 cacaatatta ttgttgcatc aaatagcatt gcctgcctgg cagcgtataa aaaagccagc
    18241 gaattgggct atacaccgct gtttttgtca tcaatgattg agggcgatac cggggaggct
    18301 gcgtgctttc atggtgcgat agctcatgag attattaaaa ctggcaatcc agtgcaaaag
    18361 cctgcctgta ttataagtgg cggtgaaaca accgtggtgg taaagggtag cggtttgggt
    18421 ggccgcaata tggagtttgc catggtgatg gcaacgctta ttgaaaatac ttcgattgtt
    18481 gctgccagtg ttggaactga tggaacggat ggcccaaccg atgcagcagg tgccgtcgca
    18541 gatggcaata cgctgaataa ggcaggaaga atggggcttg atatcaatga ttacatgaac
    18601 aacaatgact cgtatcattt ttttgaacga ttaggggatt taataaaaac aggaccaaca
    18661 aataccaatg tcatggatat caggattata ctggtggtgt aaaatttttt tatttgataa
    18721 aaaaaataaa aaaaatactt gctaaacaca aggttgcttg atatcattaa tttttaatat
    18781 aaggttttgc aaggatgcca atactttaat gcatgctaat ttcaattatc aattaaagga
    18841 gtaatacaat gaaaaagaaa ggtttgcttt ttattttatc ggttgcattg atattttcac
    18901 ttcagctttc accaatagta aatgcgcagc aggctcagcc gcagcaggct gaaaaatcag
    18961 aatttaccga agcaacacgt ccggaagaaa attatctggc aaggtttcat gcgttagaac
    19021 gtgtggaata cttaatgaaa tcaaatattg ataaaatata tatgctaaaa ataattgtga
    19081 ccaactataa agatcagggc tgggaaaaag attatgaagc atgctataat ggctataaaa
    19141 aaggtatgga ttattattat aagcgggatg taatctatgc acaggtggag ctggagaaaa
    19201 accgtaaaga catctatgaa ttatacaaaa agatagctga agtttataaa caaagaaccc
    19261 aggcattgct taacgaatgt gcaagcaaga ttcttgatct ttcgcttgat gagcggagca
    19321 aatcaaatcc tgacaggaat aaggtactat ttaacaatac tgctcgtttg cggattgcat
    19381 atggacaata tgatgatgca caaaatatga tggatgatca tgtctatgat acagccgtaa
    19441 aacatttcag ggttgcaaaa tcgtatgcaa tactcattct ggaaaatatt gatcctgaaa
    19501 attcaaaagg gaagtacgat ctggacaaag cagataatat gaaccgaatt ctgaatcaac
    19561 ctcagacaca ggcaaaatag ttacaagtaa cgataataat aaaaaagggg taatgcatta
    19621 cccctttttt atgatggtat agtattgctg atgattgtag ccagaaacat aatgataatc
    19681 gtgttgtttg ctgttctatt tatttttaca ttttatactt cttgtgttca gtatgagttt
    19741 cctgtctatc gagttgttgt tgatcccgga catggaggta aagctctact ccctaaagat
    19801 tatcatggtg acagatttga tatcttgtcc atgaaatatc ttgatatgtt ccgtgaggga
    19861 gctatgtata gacagtatta tgaacatata tacacatacg agattgctgt acgtatagaa
    19921 aaaattttac aattaacgtt acctgatggg gattttaaaa agttttgttc catagtacag
    19981 aaatatacca ataaaccgtt tgaacgtgtc tgcatacaat catttgtgag ccggcaacct
    20041 tcattaaatt ctcaaatgct acacaaagat cccaatgcgc cttaccgact gtttgattct
    20101 gttttcccgg acgggacatt gcaggaaggc aggataagct atatcaatag tttacacccc
    20161 catatggttg tatccataca cttttcgtta aatgcttcac gctactatcg aggtatgaat
    20221 gcagttattg ccgcccccta tagttttatg tataagggtt tacagtatct tcagggtaat
    20281 ggtggaaaca gatcattttt ctataatagc aactatgctg actggttcac tgagactgaa
    20341 gataaatcat cctttttctg gtactgcaat gatgttatcc agtattttat ggggtatcgt
    20401 atgaccaata actatcaacc agacatagaa gctttcaggg gataccgcta caatatggtt
    20461 cagtggatat ataacgatcc tccgggatgg gctcatatag caaagtacca tccggcaaaa
    20521 actgcatacg ccgataacgt aaagcaattt ataccaggca atgctttttt taagcgtgaa
    20581 caatctatgt atgagaaata ccgaagggat ggcgggtatg agggatatgg tggtgataac
    20641 ctttatgcat caaatgaaat tatacgatat gtattataca acctgtacct gcaaggtgtg
    20701 cggcacaaag agcagcgcct tgccccgccg tatatttcca tctggtcaat acccctccat
    20761 gtcaatgcta tcaatgcata catggaatta ggatatttag caaggcccta tacacggtat
    20821 attctggata accatctgga tgatatggct gaaggtatag cagcaggtat atactcactt
    20881 tttgctggta ttgaggtttc aaacaaaatt ccatgtgcgc catatggaaa aaagattgac
    20941 cttaaaaaat atactatcaa taaaaacaat aattacttta ccattgttcg atagttactg
    21001 taaaggtggc agatagtgaa aaaattgagc attatagcag cattgatttt ttttcagaca
    21061 cttgtttatg gagataatat aagtcagcga tttattgctg ctgcagataa agctggcaag
    21121 gctgttgtca gcatccaggt atttgttgtt gaaaaaggac actaccagaa ggttgggtat
    21181 gcttcgggaa ccataatatc atcaaagggg cttgtggtta ccaattacca tgtggttgca
    21241 aaaggcacag tatatcaggt taatttatgt gatggaactg aatgtgatgt ggagccattt
    21301 gctgatggca gtttattcaa agctgatgaa aaaaccgata tagccctttt gaaggtaaaa
    21361 cattctgcgg gattgccttt aacacctatt gagtttgagg ataaaaaagt atttccaggc
    21421 caatgggtta tggcaatagg caatccgttt ggtttgcagc aatcggtaac tgctggtgtt
    21481 gtatcatcgc ttggcaggag tgatattggt tttgctgata ttgaggatta catccagacc
    21541 gatgttccta ttaatccggg caattcagga ggcccgctca tttcacttga cggtaaactt
    21601 atagggatca atactgctat acgtactcaa agcggtggat atcagggaat aagttttgct
    21661 attcctgctt tcattgtaaa acaggtggtt ggtgaacttg ttcaatatgg cagagtacgg
    21721 aggggatggt taggattttt ggttcaggag aagatgatta ataataattc accggtacat
    21781 gtagagatta tttctattgt tgatggttca ccagcatata aggcaggtat tgaggttggc
    21841 gatattgtta gagaggtgga tggtatcccc ataaaaaaga tttctgattt aatgaaggca
    21901 atacattcca aacaaattgg ttctctggtt aacatagtta ttgctcgtga tggacagtta
    21961 ttaaattttg attttgtttt acaggaaaaa agtaaaatat ccagagttaa ttattggatt
    22021 aatgtattac agcaatcata tggaattact attgaagata cgctgtatgg tgctgtaatt
    22081 accggtgtaa atcaatttgg tattgcctat aatgtactaa aaaagggtga cattatacaa
    22141 tcaattaatg ataaacaggt tacatcatta gatgatatta ttgcaatagt caaaaaaaca
    22201 aagggtacaa ttgaaaattg tgttgtgctg cgctcaggta aggtgatacg atgttacttt
    22261 aatctggatg gaactgaata atatatgaat aaacggatta tactttttat tattgcagct
    22321 ttcatgtcta tgtgtattca atcattttct gatgaaaaga aagctgaagt tatactggac
    22381 aatgaatttt atacatcgtt gcaaaatagc gatgctatca agcgtataag aacatttgat
    22441 atgcttaaag atgtaatagt aaaaggaaca ggaaatgtag taaaagttga agttgctgat
    22501 gttttgaaaa aaaaattcag aattgttctg tcgctcaatc tgcatggcat acaaattctt
    22561 tattattgtt atactgatca ggatgagtat tttgacttat tacaaccgga tgacctattt
    22621 gaatttagcg gccaatttgt tgttgcaact ccgttgaatg taaggtttga tacctttatt
    22681 attgatgtat tgcttgaaga aggtgcggtt gtagtgcatt aatattattc gcgataaaca
    22741 gtgaagacgt agtctaaaat ggatatccta tctgcatcat ctaacccaac aaaggtaatg
    22801 tcgtacaaag tatcatccga agctgttact ttaccggtaa tatcaatttt tttagttcct
    22861 aaagtaagat gccctttaac ataatgttct aatggtgtag tggtttgcaa tagggcttct
    22921 ctatctgtta attctttgac gataccttca tgccattgta tctcttcaga tgaaaaactt
    22981 ctggacgtat tgatggaaaa agtaaaaaaa gtgaatggaa tattgacctg tgccttcaaa
    23041 catcgtcgtg agggattata tactattgta tcggtatgtt gcagaagaat aattggaaat
    23101 ggagcattaa tttttcccag aatagtagta ttaaaatcat acgagccatc aggataaggc
    23161 cataggtagc attctacagt atcattttct ttcacatgtg ataaaaaatc ttctgaagat
    23221 ttacatagta aaaaaagttc attattctca atcaccgtta agagagcata tccataacct
    23281 tttttataca ctctgacggg tgtatttgtt ttaatatcgg ctgtttttgc aatgtttttc
    23341 atgggatacc cctgaatata aatatgtagt agcaattaat aatgtaaatt gtatggttat
    23401 tgttacaatt actttctcca tttactctat cactggagaa acagatttaa aatttaattt
    23461 tagtagccac cttttataga agcatggctg tgcagtcaag ggtaaatttg ttactatgca
    23521 ataaaataga tattttttaa aaaattgaaa acctgacaaa aaagaattga aaaaaaattg
    23581 ttgcaaatca tactatgtgc aaaaatagtg gtattccttc tgctacatac actatgtaat
    23641 gtagctatta ataaccatag ggggtaacag tgaataaata cgaattaata gtgattttac
    23701 gtcctactga taagctggat atactcaaag aaaagcttcg agatatctta aaaaagaatc
    23761 aggtggtcat tgaatctgat gaaccatggg gcgttcgtgc tatggcatat gctatagaca
    23821 gagcaaatga cgggtattat gagtacttta ttgtacagat agatccagct cagatcagtg
    23881 ctattattgc tgaattcaga ttacaacccg atatcctgcg ttatctattt gtacgattgc
    23941 caaatttaag caaaacagcg taaattacca tgccaggcga tctgaataaa gttatacttg
    24001 ttggcaggat tgttagggac cctgagttgc ggtatactcc agggggcgcc gctgttgttt
    24061 ctttttcaat agctaataac cgcatatatg tgaaaaataa tgaaaagcag gaagttgtat
    24121 catttttcaa ttgtgttgcg tggggtaaat tagccgaaac agttgctaac tattgcaaaa
    24181 agggacagca attagcctta gaggggagat tacaacagcg ttcatgggac gataaagatg
    24241 ggaacaagcg ttccaccgtt gaggtggtag cagaatcatt gcaattttta ggtggaaaac
    24301 cgcatgctga aacatcacca tcagtagaca gcatggaaac tggcgatatg ggttctaccg
    24361 aaccattctc tgatgatgat attccatttt agtaaaatta taattttatt ttttaaagag
    24421 gtatataaat gagtgaagaa ataaatcagg gtgaaactat acaggcacag gaaaatataa
    24481 ttccggaaga tacaaaatca tctgaagttg caagacattc agaggataaa gcattatctg
    24541 cagccgtgca ggtgcagcgt gtaccaaaat ttaaaaagaa ggtgtgtcgt ttttgcgagg
    24601 atcctaatgt agctattgat tataaaaatc cggatttact ggtcaggttt attactgacc
    24661 gtggcaaaat attacccaga cgtattactg gcacctgtgc aaaacatcag cgccatttag
    24721 cacgtgcaat taagcgtgca aggaccatag cactgttacc atttgtggta aaataggttg
    24781 tatttgctat tgtggaactt atagtacatc ttatagctgg tatagcgtta tgtgttatta
    24841 ctgctatagt agtttataaa ttgaaatata aagctctggc gatagctctg gtcatggttc
    24901 tatcggcttt atttttaggt ctatcatatt tttataatga gatgattggt cttgagctta
    24961 ttataacggg tgctgtgttt ggatatgtgg caaaacggtt ttcattccag cgatatttta
    25021 ttaccagcgt taccatagta tggcttttgc ttttgattat tcattatata ctctattttg
    25081 taaaaggaat tgatgctata tccggcaatc aaaaaattat tgaggaaatc attaatgctt
    25141 catcaattcc tgttactgaa aagcaggtcc tgtcacaaca attggaagac tttttaccac
    25201 tgatgcgcgt gcttatgccg ttttcatatt ttttcagttc tgctgcaatt tcaattttag
    25261 tctatgtatt tgtttttgcc ttctttgtac ggtttaagtt tttacagact gaagacaata
    25321 caatttcgct tttccgtgtt gatgaatggg tggttttttt gttgatagca agctggcttg
    25381 ttgttctatt aggtaaagaa ttacaaactg tttgggcgat agctctgaat attggacttg
    25441 ttttatgtgt agtctatact atacagggta tatcggttat actgtttgtg tttgaaaaga
    25501 agcaatggcc taaattgtat ggattcctta tactttttgt tacaatttta ttaggattcc
    25561 aggtgatgtt ttttgttatc accatgctga tgggtatagg tattattgat ttctggatga
    25621 atttcagaaa gattgcataa attcaatttt cggaggaatt tcactatgag agtaatatta
    25681 cagaaagatg ttcaaaattt aggtgaagct ggtgatatca aggaagtagc agaaggattt
    25741 gcccgcaatt tccttttgcc caagaaactt gttataccat ataatgagtc aagtgcaaaa
    25801 gcaattgagc atcaaaaaaa actagttaaa ataaaaaagg aaaaacgtaa aaaagaaggg
    25861 cagaagcttg cggaggcatt atcaagtgtt gccttaacta ttggtgctca ggttggtgag
    25921 gatgataagc tatttggttc ggttactgct atcgacattg ccgacaaact tttacaaatg
    25981 ggatatacca ttgataaacg aaagatagtg ttgcccgaac ccataaagaa agctggtgag
    26041 tatgaagtaa agattaaatt agaagaagga ttaacagcta ctataaaggt tagcgtggta
    26101 aaggagtaat cgcatgcttg atgttggcat gcctcctcat gatacagagg ctgaagctgc
    26161 gtgtttatct tctatactat taagtaaaga tgcattgcta aaggttatag aaatcctcca
    26221 ggtggaggat ttctatattg atgcacatag aatgatctat gaggcaatca gggagctgga
    26281 aataaaatca gttcctattg acctcattac tataaaacag cggctcattg ataaaaaaca
    26341 gtttgaaaaa gttggcgaag atcaggctct ggtcaatata taccgtactg tatctacctc
    26401 agctaatgct gaatattatg caaacagggt caaagagtta agcttacgcc gtaaacttat
    26461 tgaaacatgc accaccattg ccgaacgttc atacgataca acattggata caaaagtttt
    26521 actggatgat gccgaaaaag atattttcct cattacacag cgcagaatta ccagcgattt
    26581 tataagttta gcaaatgttt taaaagaaac ccttgaaaat atagggcaca tgtatgagac
    26641 aaaaaggccg gtcacaggta ttgccggtgg atttactgac cttgatgcaa tgcttaccgg
    26701 ctttcatcct tcagagctca tcattattgc tgcacgtccc tcaatgggta aaactgcgct
    26761 tgcgctgaat attgccaatc atgttgcatt gcgtgaaaag caaccaacat tgttcttttc
    26821 attggaaatg cctgctacac agctagctac ccgtatctta tgtattgaat cattaattga
    26881 tatgcagcat gtccgaaccg gtcagataag caatgagcag ttgcgcaaac tggttgatat
    26941 ttcgggcaga cttgagaaat caccaatgtt tattgatgac acccctggat taagcatatt
    27001 tgagttgcgg gcaaaagcac gacgatttgc ccagaagcag aaggttggct taattgttgt
    27061 agactatatg cagcttatag ttgcaaatag ccgtttagag cgtcatcagc aaatagctga
    27121 aataagcaat gcattgaaac aattagcccg tgagttatcg gtaccggtca ttgcactatc
    27181 acagctttca cgtgcggtag aatcacgtac cgaccagatg ccacagttgt ccgacctgcg
    27241 cgagtcagga gctattgaac aggatgctga tgttgtgtta tttatttacc gtgaagagaa
    27301 ggtaaaaaaa gaaaccgagc gccttggcat tgcagacatc attgttgcca aacagcgtaa
    27361 tggcccaaca gggaggatag agctgcgatt ctgggataaa tacatgaagt ttggaactgt
    27421 tgaaaaattc agagaagaag ctgaatgata tgaataatgc tgagattata ttcctccgcc
    27481 ctgttatgaa attttttgag tctgtaggca accataccct tttttttgct caaacggtta
    27541 aatggatttt taaaccgccg tttgatacaa agaatatttt attgcagatg cttgaaattg
    27601 gctataaatc actgccggta acatgcataa cgcttttttt tacggggatg gtaatggcat
    27661 tgcagattgg cagggcaatg gattcgctca tgtccggttc atcaatattt atcgggagtg
    27721 cggtgtccat tgccatgttc agagaactgt gcccggtact tacagcgctc ctattatcag
    27781 gaagggtagg ttcagctatt gctgccgaaa ttggcaccat gcgtgttacc gaacagattg
    27841 atgctctcat tacgcttgat gccaatccca ttcagtacct ttcggtgcca cgctttatgg
    27901 catgccttgt catgacaccg gtactggttg tcatcaccga tgttgtgggc tcgttagggg
    27961 gagcatttat tgcatatttt aatttaggaa ttactatcga taaatatatt gagaatgttt
    28021 ttaattattt gcaggttatg gattttttaa caggattatt gaaatcagtt ttttttggta
    28081 ttgagattgc catcatagcc tgttatcagg gtttcaatac atttggtgga gcagaaggcg
    28141 tcggcaaatc tactatccgc gcagtggttg catcatcaat gaccatactc atatccgatt
    28201 actttcttac atacctttta caatttattg aatagaatta aagaattgcc tatgaaatcg
    28261 ctgaagaaaa gaaaagaaga gtttattaag ctattgcaaa aaaacaggat accaaaaagg
    28321 attgttgatg cttttgcgat agttaatcag gaagattttt ttgatgaaat atatagcgat
    28381 gcactgtatg gaacacagcc cattcctgta gcccctggta aaacaggtga tgagccgctg
    28441 acattagcac ggatgattca ttactgtggc attaaaaagg atttccatgt ccttgagatt
    28501 ggcaccggtt caggatattc aacagcaatt atggcacacc ttgcacaatc tgtaacaact
    28561 gttgaaattg atgaagagct ggcattgctt gcaaagaagc gattttcccg cttgaagtta
    28621 accaatatca gagtttttgc aggcgatggt accactattg atgaatcatc agggattttt
    28681 aatgcagccg tgatatttgc aggttgcaca aaaaggccat tgatgctgct tcatgtcatg
    28741 aagcaggatg gcgtagtggt gtttcctatg ggtccagctc atcaacagca gattgcactg
    28801 atgcttaatc acagggatga aaaggttgaa ggcgtttttc ctgttgtgtt taaggagttt
    28861 tgcaccttta tgccgattgc tggcatatat ggatgattat atatctgatt ttttaatttt
    28921 atccggatcc tcatcgggct tgtaacagta acggcaacca tagattaaaa tctttgtgct
    28981 gtcttttaca atttttgatg catacagtgc ctcatagcgc gaaagctttg agccacatac
    29041 agggcatgtt ctttctttca tggaagcagg ggtgaaattt ttatcaacgg ttttagctgg
    29101 cattacagtg gcagctttat ttgtctcttt ttttgcagat tgcgaagttt ttttttgcag
    29161 atgatgatac agcgaaagca tatataaaaa taatgcaata cctgctccaa acgacagtat
    29221 atataaaata atctcaacca tggatgctat gccctttttg ttataacgtt aatcataatg
    29281 gtatgatccg ttactggttt catggtataa tctcctgcta tatccactac caataatcca
    29341 cattttttta taataggaat tatctcatca atggtgtata tgcgttgcag atgttcttca
    29401 ataatggtgc ggctttgtgt tgcaaatgtc aatttagtat ataccatctt tgatttttca
    29461 tcatattcat tataccatgt tatatccgtt ccttttactg tatatgcaaa aagcctgcga
    29521 tgaaagtttt tcattatatt atactgcgtg gtaatatcaa agaggaatat actgttatac
    29581 gacatgcaat tgtatgcaca acgcagtgct tttgcaaaat catcataggt aagcagatag
    29641 ttaaaggtat cgtgtactga aaatataaaa tcaactgtct ttgatagtgc aaagcaggta
    29701 atatcaccac aaaagatgtg aaaattttta ttagcccgcg tttttgcaat ggataacatt
    29761 gctattgatt tatccatgcc gtatatttca taatcatcca tggaaaattt tggcccaaac
    29821 ttgccggtgc cgcagcccag ttcaagcacc gtgcgtggat tggaaatgta cttgcacata
    29881 acgttgcgca ggtatcggta ccattcatta tagttaacat gctgaagcat gtagtcataa
    29941 tacggtgcta agtggtgata ttcgttcatg gggcaatatg cttccattgt aatttttttt
    30001 gtaagcggtt tatccggtga gcaaagcgtg ctttgtagcg gctgttgagt tcattgtcat
    30061 ctggtgaaac attaacaata actatcgccc tgctacagta ttcaagtgcc ttttcatagt
    30121 cttttactgc atgctcgtag tgtttggcaa gttcttcaag tgaaaaaagg ttggtatgct
    30181 tttcccagaa cgccagtgcc ctgtcagtat aacccaatcg ctttaatgct gttgaatatt
    30241 ttttaaaaag cgaatgctcg ttatggagta atgcaaatcc tgcatattca acaagctcaa
    30301 caaacaactg aatgttacgg ctataaagat gtttggcaat gtttgtataa aggtttgatg
    30361 gtacctgatg aaattgtttt gcatgcagca tttgataaag ggtgttaaaa aaaacaagca
    30421 gtaaaaccat tgaggttatg tctatacggt tgtgttcaat aacattgata atacgcgggt
    30481 caaaacctgt ttgctgatag gtaaaatata cgtcggggat aaggtagcca gggatatcgt
    30541 cggtacgggt aacatcaagc aaaagttctt ccagcgattt tagcgagcag cttgcataaa
    30601 tagatttgaa tattgagcgg gctggtttta gcagatcaat gacaggcatt gatacgggaa
    30661 agcccggtat gcggttaatc ctgaacctgg tttttataat gggaatatca aatgttttcc
    30721 cgttaaaggt tacaatagct ttgtagttgt ttaccctgtc agcaaaatag tagagcagtg
    30781 ctggttcatc ggcgtattca cgcatgaata gttgctcaac ggtaaagacg ttattggcaa
    30841 agtatcccat gccaagtaaa aatgcgtagt ttcctgttcc gcttgtaagg gatgttgtct
    30901 caatgtcaaa aaatatacac tcatggagat gcaggggttc tatatggtgt atgcgacagt
    30961 aatcatcaag ataaagctgg gcggtagtga tagggaagct atcactatca tggtggtgag
    31021 caaatagcgt gacggctcca tggggagttg ttatggtgct gccgccattg agtgatatcc
    31081 atgaactatc gtgcacaaca gtttcctgct tagtgtaata tatgtccagt ctgtccttca
    31141 aattcattgt gcaatatttc aagaagtgct attatttttt cttttatccc tgttcgggta
    31201 atcattggcc ctatgcaggc aggacagccg tttgtgcagg tgcaggcggc tataacttct
    31261 tttgaggctt gagctatcaa accaaaaata gcaaaaattc gctcggagat accaacgcca
    31321 cctggaacgg catcatagat aaatatcgtt ggcctctggg taaagtgtga aaggttttca
    31381 aacaccacgc gtatatcgcc atagtcgcat aatgtgtaca acggtgcaat ggttcgcaaa
    31441 atataggata gccctatgaa aacctccccg gcctcttcgc gttcaaattg ttgcacacac
    31501 tcgtcggtaa agctgcacca cactgatgtg gaatgcatct ccaattcggg aagattaatt
    31561 ttgccccagc cgatattctc atgcgaacca aattttatct ttttaaacat gatggctttg
    31621 gtgcgtacat tgacctcgcc taaactgagt gttgctattg aaaatgattt attattaaat
    31681 tcctcaagta catggatatc agttttttcc tctgcatcgg tataataatc agattcaacc
    31741 tcatggcagt aggcaatttt acgttcccag tctaatttgt caatgtagta ctgctgtccc
    31801 tgatgaatat agattgcatc atcgtgaatc atggtgggtg ctgatggata gtcaatctga
    31861 ccaatgacct cagcgtgatt atcgttatgc actatcacca cattgtcctg tgaagcactg
    31921 cgaagcgaaa tgtcatttgc aggatagata tcgctcatcc agtgaaattt tccgccagat
    31981 tcttttaaga tattttttga tgccaggtag tgcagcatct cttttgtggt tagatggtga
    32041 tgggtaaaca gttcatcagt tgaaaaggga atttcaaatg ctgcgcattt aatgtgatcc
    32101 ataagtatca gcagattgtc agggttgata ttggcagttt ccggattgtt ttcaaaaaca
    32161 aaatgaggat gtctggcaat gtactgatca agcggagagc ttgttgcaac cataatggcc
    32221 agtgattcgg cgccgcgacg gcctgcacgt cccgattgtt gctgaagcga tgcaatagag
    32281 cctggatagc ctactgatat ggcacagtcg agcataccaa tatcaatgcc cagttctaaa
    32341 gcgttggttg aaactacagc gttaatcttt ttttcacgaa gtcccttctc aatctgacgc
    32401 cgttcattgg ggagatatcc accacgatat gcagcaatag aatagttgtt gcacttttcc
    32461 tttaaatagg tagctatgat ctccacgcgc agcctgcttc tgcaaaaaat gatagttgaa
    32521 acattatttt ttacgcacag cgagcctatg cttgctgcct cttttatggc tgatgctcgt
    32581 atacccagcg ctttatccac aacaggaggg ttataaataa caaaatgttt tttcccgcgc
    32641 ggtgcgccgt tgttgtcaat gatggtaaag ggcaattcaa aaattgtttc agctaactct
    32701 tttggattat ggatggtagc tgagcagcag ataaattgcg gattgctgcc gtaaaacgag
    32761 catatacgtt ttaaccgtcg catgaggttg gcaaaatggc tcccaaaaac gcctttatag
    32821 gtgtgtattt catcaacaac gatgtattga agattttcaa aaagcttaac ccatatggta
    32881 tggtgaggca aaattcccga atgaagcata tctggattgg taatgacaat attgcctgct
    32941 gttcgtattg ctttgcgtgc agattgtggt gtgtcgccat caaaggtata tgttttaacc
    33001 gatagtcccg caaagccaat gatctcctgc agctcagaaa gctggtcctg tgaaagcgct
    33061 tttgttggga aaagatacaa tgctcgtgca tcaggatgtt ttactatgtg attgagtacg
    33121 ggaagattat agcacaatgt tttaccagat gctgttggtg ttacaacaac aatgttttct
    33181 tttcgttgta ctgcatcgca ggcgctacgc tgatgggtgt aaagcttttc aatcccgcgt
    33241 tttttataca ctgctataat tgatgcatca caattatcag gatataaggc atattctgct
    33301 tctttagcag ggatggtatg ccatgcagca acctgtttta taaatggttc ataatttgta
    33361 agatattcta taaactgttg aatattcatt gttatggata actcacacga tattttgtta
    33421 ctatcatatc cggctggtat gaacgctttg caaagcgcag agcttcaaca cctaaatcct
    33481 gttcaaagtt aaaaaattta tagttagctg gtaatgcatt tgcacagtgg ttgtaaagat
    33541 actggtatat tcccttatag cttgttatag cttttgcaaa atgtattgca aacatatcaa
    33601 cggtaattgc ttcgccaata ataaatgctg ccggtttatc gtcaacataa tatatactgc
    33661 cgcacagatt aagttcgtca agcaacatgc atgcttcact acaggcgcta acatcagccg
    33721 aggtgcctga ttcttttttc cattcatcaa gtacatacag cgcatcgttt atccgttcgg
    33781 atgtcagtgg caaaacagtg gtagaatatt ttgctgtaaa ttgatacagc aggtttcttt
    33841 tgccatgcag ctttttaccg gggtatgtag ctaatttttc ctttgtgtaa acatagtccg
    33901 aatcgccatc gttgtagtag tagctcaacc ctttttccgt aaaaaaatga agccaatcat
    33961 cagcaattgg gaaaagacag cttccgggaa gtaacagttg cgttagcttt tcggcatact
    34021 cacaatccag gggtttaact ggcatgatgt agcttgtacc atcataggtt atgccccgga
    34081 ccgcaacaat atctccgctt atatacgaat agttgtgttc ttttctaaac agatataaat
    34141 ttgcaaacga atattcagaa attggcagtt gaatctggct taaaagcggt tgtaaaatag
    34201 ctttgtgcga tagttccagt gattgttctt ccattgccat aactaatccc ttttatcaat
    34261 atgcctttgt ccgggcatag tacggagtat tccttctttt tcaatcttat ttttttattt
    34321 tataagtgct ataaaatttt tataaatcta ttgacgatag tattatttgt cagtatattt
    34381 ctcattaatt ttaaatagct gcaataagcc gagatccatg aaagcagcaa tggatgcggg
    34441 ggaaccactt tatggggcgt attccttgcg aaggataggg tacttccaaa cccaagcccg
    34501 tcagctaacc tcgtaggctt ttggaagggc aagagcgtta aactgctttt tgcagttttt
    34561 tttatgtcct tgcccccata agggggtatt tatgagcttt cgcaaaaaat ttttgaaagc
    34621 agtatcacct tttcaaatac tggtattggg atatctgcta ataacagttt caggtgcatt
    34681 ccttctttcc atcccatttg cttcaaaaga cggacaatgg cacaattatg ttgattcgtt
    34741 atttatggca gcatccgcca taagcaccac cggtttggtt gtagtagata ccggcagttt
    34801 ttatacatta tttggacagt tagttttaat gactattttc cagattggcg ggataggata
    34861 tatgtcgttg ttgatgtttg tactgtatct ttttgatata aagacatcaa taactgtaca
    34921 gctaacagca aaagaatcac tttcgggccc tacgcttaga atattaggga aatttttttt
    34981 agttactgtt atatttacat taatctttga agcaagtggc gcaatagttt tagcattgta
    35041 ctggatgcaa aaatataata gtataactgc tgtctattat ggcgtgtttc attcaatttc
    35101 aactttctgc acggcaggat tttgtttatt ccctgacagt ttaatgagct ataaagatag
    35161 tattataata aatggttcaa taaatttttt atcacttgca ggcggtatag gttttattgt
    35221 attgtatgaa acgtttgcgt ttgtttctaa acgattgagg ggtataaaag ttaaacatgt
    35281 atctgttcat acaaaagtag tattattgac aacttttatc attgtaataa ctgctacact
    35341 attactactg gtaaccgagc attgggatta tagtacaagc attatacaaa gatttgtcta
    35401 ttcttcattt caggtaatat cagcatcgac aactgatgga tttaatacca tagatattgg
    35461 tggaatgagt tctgccagta ttataattat aatgttactt atgtttgtgg gtgcttcacc
    35521 tggcagcaca ggcggtggaa ttaagacaac aacatttgca atcatatgca tgtatattgt
    35581 atccttattg aagggaagga gcaatggagt tgtaaatatt tatgaaagag aaatcccgac
    35641 agaatctatt aataaagcgc ttggtatttt tataatgttt attatagtaa ctatgattga
    35701 tttgatatca atgagtatga ccgaaaaatg taattttgaa aacattttat ttgaaataat
    35761 atcagcttta ggaaatacag gattatcaac agggataacc tcccaattaa gtattaccgg
    35821 taaggtttta ttaacggtga caatgtttat aggccgggta ggacctataa catttggttt
    35881 ttttattctt ggtaataaaa aggtacagat tgttcactat ccgcaggagg atatatttgt
    35941 tggttaaaga tttatgatat tttttaaata atgggcaaaa cattattctg taatgataat
    36001 tatttatagt ataattatta ccataataga taaaaatttc cttgatgata aaaacgagca
    36061 cacaatatta tataaaaata ttttctaaaa agtatctcta ttaataacgt ttttcattat
    36121 taaaacattg ttaaaatatg atacaacatt tttcattact tttccacaat ggaatatcat
    36181 aaatctaatg atcaaaggtt ccctgatatt taggcgtata cattgttgta atattacaag
    36241 gtaaaagtta aaaataaact atgaatgcaa ggatgtaata tgccgggcac tcttaaagga
    36301 taaaaaatga tacaaaataa acacgatcag gcagcagcaa tagaagaagt atcggcttcc
    36361 atagaagaat tatctgctgg taatgaaaat gttaagcttc atacagatag gcagtttgac
    36421 aggattgcca ttttatcaca gattatgata acagcttctg atcttattaa agagatgata
    36481 gatcttactc agaaagcgct cacaagaatg caggatattt ttaaaaaagc acaatccggt
    36541 gaggcatcac ttgctcttat gagtgagtaa atgggaacta tcgccaaaag ttctggtgaa
    36601 atcaacggaa tcattgcaat aataataaat gatatatcag acagaattaa tttactgtcg
    36661 ctcaatgctg ctattgaaac cgctcgtgct ggggatcata gaagaggttt tgcagttgtt
    36721 gcaggtgaag tatcaaaatt agccgatcag acagctgtaa gtataaaaac cataggatcg
    36781 ctaataataa acaatgatcg tgaaataaaa actgggatgc aaaacgtttc tcaggcaata
    36841 gggacaatta atagcataat taaagatata gatgttatcg tttcacatat cagtgcaata
    36901 tctgatactt ttaaggcagg ccgaggaaaa taataaagct gtgaaggtgg gaacgctgtg
    36961 agtttgcttt cagagcagat aaaaattgca atggatgaac aaaaatcagc aattagcgaa
    37021 atagcaaaaa cggtgagttc tatcaatgaa ttagcacagg agaatactgc aaaaggattg
    37081 gatattaccg aaacttccag agcacttgtc ggcaaagtgc aatctataaa taaagagata
    37141 gatgagtttt catctggaac ataggtatct cacggtatga tggtaaatgt aaaattttca
    37201 ttttactgcc cccataatgt atactattga tacatctgat attagcaaga tttttgccgg
    37261 attagatagc ccggtttttg atgtacccat aaaataacta ttgacaggag ttgttccctg
    37321 ataaattgta acttttcaat aattaatgat ttaagataca tatattttta gctatatacg
    37381 gagataataa tatgccgttt tataaattta taccccatga aataaaattt tttgatttgt
    37441 ttgatagaca ggctgataaa atggtagaag ctgtcatatg ctttaaggaa tttgctgaaa
    37501 aaggaatatt tgacgatagt tgtattgaaa ggatgcgttt gttagagcat caaagtgaca
    37561 gcatcgcaca tgatattatt gatagcctta acagaacttt tataacacca tttgataggg
    37621 aagatatcca ctcattaacc aataagcttg atgatgtggt tgatttaatt tatgctatat
    37681 caaagcgtat gcggctatat aaattaaaag ggaaaaatgc agatgttcag gagttttcca
    37741 ggctcataat gcaatcagtg caatcattat ctcttgctat aaaaggtttg cgaaatcaca
    37801 gacagccaca aataattcag caagcctgta ttgaaataaa taaaatagaa aatatcagtg
    37861 atcagttgcg cgatgtggtt atacagaggt tatttaaaag taaaaaggat cctattgcaa
    37921 ttatcaagtg gaaagaaata tatgaattat tggaaactgt attagatgtc tgtgaagatg
    37981 tttccaatat tgttgtttcc attcttgtta aacaaggtta aatctttatg actatctata
    38041 tctttttttt gattgcgctg gcattgctgt ttgatttcct taatggtttc catgatgcag
    38101 ccaattcaat agctaccgtc gtatctacaa gggtactatc accccgctat gctgtattgt
    38161 gggcagcctt ttttaatttt gttgcttttt tattttttgg actgcatgtt gctggtacca
    38221 taggcaatgg tattattgat gtttctatca tggataatac agtaatactg gcaacactaa
    38281 ccggtgcgtg tatgtggaat atcataacat ggtatttagg gttgccaaca agttcatcac
    38341 atgctcttat tggagggttg attggcgctg cattgatgaa agttggcacg aaagcattgg
    38401 tgcttgatgg tattataaaa actgttattt ttatcttttt atcgccgata gttggttttg
    38461 gattagggct tgtttttggg actgcagttt actggatatt ccgacactca catccagcta
    38521 aggttgataa aatattcaga aaaggacaat tgatatcggc agcgttctat agcctgggac
    38581 atggcggaaa cgatgcacaa aaaactatgg gtattatagc aggtttgcta tttagcaatg
    38641 ggctgatgca ggttgctgaa gggcagcgat ttcaggatgc tattccctta tgggttgtgc
    38701 ttgcatgcca tagtgcaatt gcattgggca ccatgtttgg tggatggcgc attgtaaaaa
    38761 cgatgggaca gcgtgtggta aagttacggc cggtagacgg tttttgtgct gaattgggag
    38821 cagcttttaa tctttatgtt ataacatcat attttggtat ccccgtgagt accacgcata
    38881 ccatcactgg cgcaattatg ggggttggaa ctttacgccg atttactgct gttcggtggg
    38941 gcgttgcagg acagatagta tggtcatgga tactgacaat tccattatct gcactcattg
    39001 cggcaataac atacagtatt gttacataat ctcagttgca ctattggttt atagccttgt
    39061 tgtatcactt tttggataca tcaaatcaat caaatcttta tatttttcat gtatctggct
    39121 gcgtctgaat ttaaatgttg gagtaagctc tcctgttttt acagtaaagt catgcgctaa
    39181 cagcgtaaat ttttttatct gttcataagg agccactgtc tggcatattt cattgattct
    39241 ggttttaaac aatgtttgta tctggtggtg tgatacaagc tcttcatcag ttttatagct
    39301 tattccgtgt tcacgggcat attgccgtaa aagatggaaa ttagggacta tcaacgcaga
    39361 caaatatttt ctgttatcac caacaacaca tatataattt attaaaggtt cggcttcaaa
    39421 tttcttttca atatgcaaag gaactatatt cttaccctgt gaggtgataa tgatatcttt
    39481 gagccttcct gttattcgta aatagccatc ctcatctatt gtgcctatat caccagagcg
    39541 gaagtagccg ctggcggtaa aaacttccaa tctttcttca ggtgtcatct tgtgatatcc
    39601 cttgaaaacc attggccctt tgatgagtat ttcaccttcg tcagatattt taacttctgc
    39661 accggggatg ggatatccaa cagtaccggg cttgtttttt tctatacggt tgatagtaac
    39721 tgttggagat gtttcggtta agccgtatcc ctcaataatg ggaagtccaa tcccaccaaa
    39781 gaaaatagat acatcaatag gtaaaggagc accacctgaa aaaagtagcc tgacctgctg
    39841 catgtgcatt aacttttgta tacttttagt aattggtgat aatagtttat taaatagtgc
    39901 agcaccttta ggaatagttt taccagatgt ttgtgcaatg tagcatcccc ttccaatatt
    39961 gatagatgaa aaataaataa atctttttat tggattgaaa cggcgaatag tatgaatggt
    40021 atttatatag agcttttcat acattctggg gactgtagca ataaccgttg gttttatatc
    40081 gatcatgtta cgataaatct cttcactttc aagagtggtt gagatggcca ttggtgcacc
    40141 ggtaaatgag cttattaata aatcggcagc aaaaccataa acatggctaa atggcaggtg
    40201 agccaaccgt atatctgcat cggaaaattt gaatataggg attacaacat gctgtgagat
    40261 aaagttgcca tgagtaagtg gaacgcccct tgggtcacct gatgtacctg gagtatacat
    40321 aagtgctgca taatcttcgg aattgatttt ttctattgct cggttaattt cttcgtaatg
    40381 agctttttct tccccaaatt gtatcagttc atcaaaggtt acaagctgct catgctttgt
    40441 tttagaatca aagtatgtta taagtcttcg caggggagat tgtactttat ttaatatagc
    40501 aaagcttttt tgaaactgtg cagcatcgcc aacaaagatt gttctacatc cagaatcacg
    40561 cagtatttct tccagttcat cctgtaagat ggtaggataa attggcataa ctatcgcccc
    40621 aagggacatg acggcaaaaa ctgttgccgc ataatataaa ttattgcggc taaatatacc
    40681 tactatggta tctttttcaa ctccataggc taatagtgct gaagctatat tatactgaag
    40741 ggttaaaaac tcttcccctg ttattgtaac aaatttatca gggaactctt tattgtaatt
    40801 cttagcataa aaaacagact tgctaaaatg ttttctgtaa gaatctattt ccaaagaagg
    40861 tatgttttta tcagtagctt caagctcttt aattatttca attaactgca tatattactc
    40921 ctgaaagtat tatattgcaa cagcatttga cattctttga aattcatata gtgcatagta
    40981 tatttgtata tatgattttg cgtcaacaaa ataagataat tttcacaatt attttatcat
    41041 gaaagttgtt tttctgcaaa aaattgtaaa atatgaaaat atgatggaag atattgaaaa
    41101 ctttactttt tgattccacg gtgaataaaa aatgaagata aaatcccttg tttcaattgt
    41161 tcatcagatg aaaatataag tttttagagt tgtccataaa tataaaaaac gtcaaactat
    41221 aaaactcttg actataattt agttattttt ttagtgtaaa taaaaggagt tacgtgatga
    41281 aaactgctaa cattcctgaa ttgattgaag agatacgcaa tggaagaatg attatccttg
    41341 ttgataatga ggatagagaa aatgaagggg atctggttgt tgccgctcaa tattgcactc
    41401 cggataaaat taattttatg gctacgcatg gcaaaggctt gatatgtatt gccatgacac
    41461 aggaacgtct gcaagaattg ggattgccac tgatggttcc cgaaaattca gataagtttt
    41521 gtacagcatt taccgtttca gttgatgcca aagaaggaac gtcaaccggt atttctgcct
    41581 atgatagggc aaagacggtg catgcattga ttgatccacg caccaaaccg caggatttaa
    41641 acaagccggg gcatattttc ccccttactg cacgaaaggg cggcgtgctg gtacgagcag
    41701 ggcatactga agggtctttt gatttaaccc gtttggcagg tttatatcct gctgctgtta
    41761 tgtgcgagat attaaatgat gatgggacga tggcccggcg ccccgatctg gatgcatttg
    41821 ctgaaaagca caatttaaaa attggaacca tcgaagattt aataaaattt cgccagcata
    41881 aagaacgtct tgtacataga gtttcagagg cacacttacc aacggagtat ggtgacttta
    41941 aaattattgc ctatgaaaca gaggtggata atgctacgca tgttgcactg gtaaaaggag
    42001 aggttgatgg cagggaaaat gtacttgtca gggtacattc agagtgttta accggtgatg
    42061 tctttggttc gcacaggtgt gattgcgggt cacagttgca tcaagcgctc aggatgatag
    42121 aaaaagaagg gttgggcacg ctggtatata tgcgtcagga aggccgcggg atagggctca
    42181 gtaataaatt aaaggcatat catcttcagg agcaggggta tgatacggtt gaggcaaata
    42241 taaagttagg atttcctgct gatcttcgta attatggtat tggggcacag atacttgttg
    42301 atctgggact gtcaacaata cgtctgatga caaacaatcc aaaaaaggtg attggattgg
    42361 aaggatatgg gcttaccatt gtggaacgtg tccccatcga aattccacct ataaaagaaa
    42421 actatcgcta cctaaaaaca aaaaaagata aattaggaca tctaatatta caagctcaag
    42481 aggataagaa gtcttgaaaa atggacaggc gcccacaata gtagttgttg acgatgatga
    42541 ggtaattcgc tatagcctcc agaaaaagtt gtcaagatta ggttacaaca ttatttcatt
    42601 aagcaaagcg gaagatgctc ttttcctgtt gaaaaatgat gaacgggaga tagaccttat
    42661 aataacggat ataaaactta gaaaaatgga tggtattgag ttattgcgtc atgttaatgt
    42721 tagcgaaaat cctgtaccta cacttattat aacagggcag ggaaatattg aagatgcaat
    42781 taaagcgtta cgatatggtg cactggattt tatcagaaaa ccatttgaca tgaatgaggt
    42841 tgcttcggtt gtcaggaata ttttgagaag aagacaacag catagccttg agaatacatt
    42901 gggacagtat attgttgatg atcatagaac gcttgtcatc ccaatagatg aaacaattgc
    42961 tgatcttgtt gcctacgaat taacaaaaca cttatcgtat gcaaagtttt gtaatcgttc
    43021 aacggctgaa aatatatcaa tggcacttct tgaagcgctt tccaacgcaa tgtaccatgg
    43081 caatttagag ataccctctg aggtgcgtga ggagcacggg gtgaaaggat ttcatgatga
    43141 aatacaaaag cgcttgcagg attctcagta taatcaacga aaggttacca tacgttatag
    43201 ctatacaggg gaatatgttg aatatattat agaggatgag ggcaaaggtt ttgattataa
    43261 aaatgttgca gatccacgcg accccgaaaa ctttttgaaa aatagcggga gaggactttt
    43321 tattattcag gttcatatgg atgaggttga atgggataat ggtggcagac ggatacgcat
    43381 gcgaaaatat aaaacactgt aatgaggtgc taacatgtac tatactataa aatgggatga
    43441 tacagccgtt tcaatgcttg atcaacgtat gttaccggta caggaaattt accggcatta
    43501 caccaattat catgatgtag ctgatgcaat tcgttctatg gttatacgtg gagcgccagc
    43561 tattggtatt gcagcagcta tggggatagc ccttgcagcg gtaaaaaaca agctatcagt
    43621 tcatgatgaa tttaaaactg agatggtaag ggtatgtgat gagtttgcac aaacccgccc
    43681 cacagcagta aaccttttct gggcaataag ccgtatgaaa acaattattg aaaaggcaac
    43741 tgatattcag cattgcaaag aagcccttgt tgatgaggca aagaagattc atcaggaaga
    43801 tattgccata aacaaaacaa ttggtgaaaa cggacaaaag tttctggatg atggtatgac
    43861 ggtgatgaca tactgcaatg cgggtgcgct ggctaccgga ggttatggta ctgcgctggg
    43921 tgttatacgc agcgccatag agcatgggaa aaaaataaaa gtgtatgcct gtgaaacccg
    43981 tccttactta cagggtgcac gtatcaccgc atgggagctt tataaagatg gcattgaaac
    44041 aacgcttatc tgtgataata tggcagggta ctttatgaaa aaaggagaaa ttaactgtgt
    44101 gatagttggt gctgatagaa ttgctgcaaa tggcgatacg gcaaacaaga taggtaccta
    44161 cacaatgtcc ctgcttgcaa aggcgcatgc cattccattt tatgttgccg ctccgctatc
    44221 aaccttagat acaaatacca ttaatggcga tagtattatt atagaagaac gcagcattga
    44281 tgaggtggca tatattcagg gtgtgcagat tgcaccggag ggcatattta ttaaaaatcc
    44341 cgcatttgat ataacgccgg ctgacaatat aacagctatt attactgaac agggaatagt
    44401 agagcatcct gctgcaactg gcatagcccc gctttttagt gtgcgatagt tactattaaa
    44461 ttaaaagctg tataccactg caataaaata aattttttta gttgctttta ttcataatgt
    44521 gtaggagtgt aatctatgtt atagcgaaaa acagccatct gggtgaaatc gttaaaaaag
    44581 agattaaaac tcttgaagcc ttaaagcaat tgaacgggta gcagtttaaa tttgtgggca
    44641 atgtcattcc cgcgaaggcg ggaatctaat aaggcatgtc gagtaaaaat gaataaatat
    44701 tacatatata tgatcaccaa tagaaataat aatgtccttt tcattggtgt aacaaatgac
    44761 ttgaaacgaa gggtttttga gcacagggag aaactggtgg aaggatttag ctcaaaatat
    44821 aattgcagca aacttgtgtg gtatgagcaa acgaataata ttggaagcgc tattctaaga
    44881 gaaaagcaga tgaaaaaatg gaaaagggaa tataacaata acattataaa tgagatgaat
    44941 cccgaatgga atgatttata tgagactatt ctatgagatt cctgcattcg caggaatgac
    45001 agaaagtatg caggaatgac agaagtgtat aaatctatta tgggaggaat tatgaggcag
    45061 gctatcagta ttatacctgt tatacttagt atggtgctgt atggtacaat cggtgttgca
    45121 agggacattc cctacacact tgaagataga gaacggttga tacgggttga aacaaaatta
    45181 gaagaaggct acaaggcttt gcatagcagg attgatgcgg tggataaaag gattgatgcg
    45241 gtggataaac gcatagatga tgttaagggg ttgctgtacg ttttaatagc agcaatattt
    45301 gcacaaactg ttggagtagt tggctttgta gtatgggacc gacgaaccgc attatcacct
    45361 gctattaata aaacaaaaga gattgaagaa aaagaagata agattattac tgcattgaaa
    45421 caatcggcaa tgaaggataa gaatattgca gaagctttgc gaaaggcagg gatgctttaa
    45481 tagtccaaaa tgtccattat atgtaatagt aatatagtaa tattatctct ttcccataca
    45541 gtagtgctgc tataaatcct aatgtaagaa acggtgcaaa tggtattttt gctttcaatg
    45601 tctttttttt tataacagca aaaagtattc caaacaatgc gccgctcaca agagctatct
    45661 ccagagctac tatcgtttct gaaaagccta aaaaaaagcc aattgcaaca gcatatttta
    45721 tatcgccacc gccaaacccg ccttttttta tcactaacgg cagtataaaa acaacaagca
    45781 tgagcgctgc gccttcaatg tgtgaaacca cttcaccttc tatagcggtg atgatgatac
    45841 ctgcaatgag gattattaaa acaaggaggt cagggatctc catggttata gtgtcgatta
    45901 tagctatgga tacgcttgtt gtgaaggcaa caaatagcac aggagctatc gccagtggga
    45961 attgaagaac agcaataaca gcaacaatgc cgcaaaacag ttcagaaagc gggtaacgta
    46021 gtgaaacagg ctttttgcag taagcacagc acccctttgt taaacaatag cctataacgg
    46081 gaataaggta cactggcttt atttttttat ggcagtgtgg acaatgcgag ggataggtca
    46141 gtgcatggca gggattgttg gtgtacatgc cgttggtgta gcggatgata aatgtatata
    46201 aaaagcttcc ccacgaggct ccaataataa atgcaaacgc aacaataagt gtatcattca
    46261 tacgtaattg ttaatccatg tggataaaag ttatgttaat ctattggtaa cgatactata
    46321 tcaaaggacg tttactcaat agcataaata tgttattgta attatgcgat gctgaatcgg
    46381 gtgtaaagct ataacgttga cgatgataat gtttatctcc atttatgtaa gcaagtatta
    46441 tagttacatg ccaatatagt agttaacacc aaaaagcaaa aaatcgccct ttaccgagat
    46501 ggtccgctta tctgtgcctg aaacttcatc tttaataggg atggaacgtc cttcctgatg
    46561 catccattcg actgaaaggg taatttttga tgtaagcggt gcttcggctc ccatgatgta
    46621 atgaaaacca gggattgaat ctgcataacg aactttgaag tcgggattgg tttgtttaat
    46681 ttcaagatag gcatagttat aggtaagccc tgcccccagg aagaaatacg cacgctgctt
    46741 taacggcata ataagaccaa cggttgcagg caatccaaac tggtaggttg atatttttat
    46801 tctggtgcta tccgcttcac ctctcacgct gtaggggctt gtaaaatgtg cgccaatcct
    46861 gaaaagaaaa tagttaaact ggtagcgtac attaatcccc catacaaagg aataggggat
    46921 gcttatctgg tcaacatctg atgcagttgt tttggcttga gtattccatt gggtaatagc
    46981 tggttctaaa tggttgggat cctgggtaag gccagcatta aggcctatgg aaaactcgcc
    47041 aatggcaaat gcgtgttcat gttctataaa tataaataat agcagggcga tagttattat
    47101 aatagtgtgg tgtactgttt tcataatgtt catagctaaa ttggtttaaa gtaatacgat
    47161 atccctaaaa gaaatcgata gcctgtgaaa tccaatggtg cataatctgt attgccatca
    47221 ccttgttttg aatccttaat aacggttgtt gtaccacgat aatatgataa cgatgttgca
    47281 agtgccacct tttcatccat gaaatattcg ccttcaacat ttaataccag tggataatca
    47341 tgccctttaa attcgcctct gtagagcaaa ggtgcatcgg attcaaactt atttcggtat
    47401 ctgccccatg catacgcaat gccgcatgaa aatgttatct tcatgtcctt gtaaaacggt
    47461 ataacaatgc caaaagttac cggtatatca aagccatcat atcggtaggt gcattccagt
    47521 aatcgggtgg taccgccatc atcatatact gttttgccct tcccgcccca tacgttttta
    47581 aaatagttgg caccaatacg cagtgcaaaa aagtcataca ttacaatctt aagattaatc
    47641 ccggcagttg ctcctatgag cctgtgtatt tgcgatgtat taaatcctga aagagatttg
    47701 tttattccgt ctactccatt ggtggagccg aaggtatttt cctgttcata tgaataaagg
    47761 ttatcgccta atgacgggat ataaccggca taaaagctaa tagcctggtt aaatggcgaa
    47821 gcatatacag caatgggtgc gagagctatc gccgccataa aaagtatttt ccgtaattta
    47881 taatacatag tacattacac ctaacatgat tctgtgaccg ccataattga taaaaagatc
    47941 ttcatagccg tttgggttca atgttcctgt tgttgttatt actggatctg attgagcgtc
    48001 tatatacatc cattccatgt acagtgaggt ggagcttgtt atatggagct gtataccagc
    48061 taaaaatcca gtaccaagac aggttgcatc gatatcttta gatgaaccat cagaaagcct
    48121 tacgttgccg gtgccgtaaa aatacgaaat gccaccccct aaataaaata cacctttatc
    48181 aagcagtggg aattgcaagc ccgcatacaa cggaatcata gcatatttta cattatagga
    48241 tttaacgcca acggtagcct catcttcaag aaccttacct ttattgaggg taaatgatgt
    48301 atccgcaccg gtgcggataa aaaagtaagg tattgacagc cttactgcaa ttccaaaaag
    48361 aatactgttt tgagggtcgg tctttgcatc aggataaaag gtggttatat caccaacgtc
    48421 atgttgtgca ccaacatgaa tacctatgcc ggcataacgt tgtgcataac cggtaacaat
    48481 acctgtatgg agtactatta ccagggttgt aaggataata gcttttttaa ccattaggaa
    48541 ccttcttaat agattacatc gacgataatt gagaaaatca aaagtaaaaa agtcaatact
    48601 ataattatta agggtttaca cggttacata ttcttgaaaa ggtcagcagc tttatttttg
    48661 ctggatagcc tttcataatc atcataatcc acaaagataa ggtgtggtaa tttcttttta
    48721 aggcgcataa caacctcagc gcttttgcca ccacgggcat cctttgatgt tacatattta
    48781 aattctctat tacatgttgg gcatgtatgc aaatcctgca gtttaagccc tggtgtttta
    48841 caaccggggc aaaatccaaa aaggtcatca gccattaaca ggtgctgttg aatgtccttc
    48901 atatcggctt cctgccatat ccgtaataag tccatagcat atatccttaa caattttttt
    48961 caatttcaat ggtacgatag ttattgtgaa aatgcaaata ttttttacat tattctgttg
    49021 ttaaaaaatc ttttagtttc atcttcttaa cacgtaaagg cttttcctgc attaccacta
    49081 caacattttc ttcattatta aaaaattgtg atgtgtaatt aatgtttttt ttacattctt
    49141 cattgacaat aaaaccaata gactttgctt taatggaagc ttcttccatt tttggaaatg
    49201 tgatatcact gcccaatttg aaatttgcat cccagaaaat gtgagtgaaa attttaaaag
    49261 ttttataatc gccctgctgt actcttttat aacagtagag ataatccttt tcagtaacct
    49321 ttgctttctg gcatgcttcc tgcaatgaat atccctggtt cagaaaaaac ataactgcct
    49381 ctaccggttt tttccatctg tcggcaacaa ccttttcctg aatgatgggt attccgtgtg
    49441 catacgcatc gtttagcaat tggtaataac ggaatgtttc aacaaacgga tgctcacgca
    49501 aaaagtttga acgggctgcc tgagggaagt gcggcaaaac gataatggtt gcaattgtgc
    49561 tcactacaga caatactact gtaatccatg catacattgc aaccgacgat tgcgcggcag
    49621 gtgatatatg atggctggta gtggatgaca tgcgcaaaga atttttaaca gtaactatcg
    49681 catacgccag tataaaacac atgaatggtg cagcgggaac aagatgtcgt ggattaaacg
    49741 acattgcagg gaccaccggc acgatgctct ttactgcaaa tgttaacaga ataaagaata
    49801 caagcggtat aatgatgagc gactgaatgg taacatccgg ttttaatttt ttcatacgaa
    49861 tgatgagata gcatgtgctt agtatgtatg cataaaaata taacttttca aatacattga
    49921 gttgagcata tcgtgacaat aagctccaca cggagggtag tgccatgagg ttgccactgc
    49981 ttaaatgcga accagctaca atttgtgctc tgcctaatgg catccctgct aaataatagt
    50041 aaagggcggt ttcgcataaa aaaagaacaa acagcacaaa accaaaaaat agcacatgat
    50101 taattttcct tttcatcatc catatacaga tggcaaaacc tggaataaag aataatgctg
    50161 tttctttggt gaggtatgcc ataaacatga aaaacgcaga taccagaaga taccagtatt
    50221 ggtgtttgct ggaatcatga aacttaacaa tgaaccatgc gcttatcagg atgtatgctg
    50281 ctaaaaatcc atccggttta agttgcactg catggctgaa catcttggga tacaatatca
    50341 gcagtatgga actaagaaat gctatattga ttccacatgc tcgtactgca attttatata
    50401 aaaaaactat ctgaagaata taaaacaaga gtggcataat ataatacaca atggggtgag
    50461 tgccaaaaat gcactgactt aacgttaccg gcagtatcat gccaaagcgc acagtatggt
    50521 gattaagaat atatggcagt ccatagtaaa gccgtttggc agcaaaccag tagcttgcgg
    50581 cgtcgccgcc tgtttcaatg ttggtgatgg taagaaacct gaataaaagc ccaaagaaga
    50641 gtatcagcaa aagtatgatg ttttctttgt taaaaaaaga cgagaaccat gtgatactgg
    50701 catgtgcagt aatttttttc atgcttattt tttccttatg caaaagattg tacaatcatt
    50761 atttcattat tcataaccat tatctgaata aacatcaatt tcatgatctg gaaaaaaggg
    50821 ataaatatac aataaaattt ttgtaaaaaa tcatgtattt agtttgcgat agttactgct
    50881 gatatagtca tatgtgcccc attttttcag ataaccgctc aggtaaactt ttttttgaaa
    50941 aaattggaga ttttgtttga ctttccagat gtctggtcta ttatcattta tgttgatgtc
    51001 aataaaatgg ttgccgatta caaagcaagt gtgtataaat ccttagggga tattaaaaat
    51061 taattctata ttgtgcttat ggcaaaaaaa gacacatccg attggaaatt tatcgtcgtc
    51121 aacacactgt tctggctact cctgggcgga ctgctgtttt ccaataagag tcgcttcaca
    51181 ataggcggag ccattctgtt ccttattata ggcagcctca ctatctattt agggcttaag
    51241 aaatcaggtg gactaagata cccgggtctt gtccggataa tggctgtagt gtcgggaatc
    51301 atctacattc tcaccagcgg gtggttgttc atcgattact tcacaagcga tgaacatatt
    51361 agcatgaagg gtcgaatcgc acaggttgcg aataaagaaa cgaccactct ggatctgtcg
    51421 ggaagaaaat tgaccgctct cgatccaaaa atcatggagc tttcgacact aacatttctc
    51481 aatctggaaa acaaccgttt aaccgaactg ccgccggaaa ttggtaggct ggtcaacctt
    51541 acattcctta atctggaaga caatcaactg accgaactgc cgccagaaat tggtaggctg
    51601 gtcaacctca catttcttaa tctggataac aatcaactga ctgaactgcc gccagaaatt
    51661 ggcaatctca ctaaccttaa aattctctgg ctgcgtaata accatatcac caatctccca
    51721 ccagaaattg gcaatctcca caagttagaa tctctggcac taagtgacaa ccagctatcc
    51781 accctgccat ccgaaattgg ccaactttcc agccttcaac accttgctct ctatcgcaat
    51841 caattgactg tgctgccccg tgaaattggg cagctctcga accttaccac tttgtttctt
    51901 gacgataatc aactgacaaa actgcctcct gagctggagc gacttaccca actacagact
    51961 ttgagtctta accgtaatcc attgggagag ttgcccgata ggttcatgga gcaggcgcaa
    52021 tccagggggt taaaaatcag ctataaacca gatgaaaaac tccccccaca gggagatggt
    52081 ggtttactgt tgtttctggt tgtctttgtc gccatcattt tgataacctt tggtcttaac
    52141 agatggattg gcaaacggga agaacagcta aaactaaatc taaacacttc aggaagggtc
    52201 tatgcgattc cggcaggtgg cagagcggga tgtattttcg tgttgttgtt gaaaataatg
    52261 gtggacgtat ttctgttcat agcttctctc catcctgaag aaaccggcgt tgcgccagca
    52321 gcgggaatcg gcattccact tctgttttca ccattgatta ttatctgttt gtacatactt
    52381 gtgcactacg atggattcat cgttttgaca ggagatgcta ttattttgcg acgcctatgg
    52441 ggtgagaaac gattgcccta tcacgaaatc accgacgtca aggaatcaag tttgtggttg
    52501 ccttttaaca tgattatcaa aggaactcat acgaaactga gtattccgcg ttcaatagaa
    52561 aaatttcccg aactctacgg agcacttatg caacagatca ccagaaaacc cagcgttcct
    52621 gatatcacca ggaaattcga tgctccttca ttcccctatc atttagcgtt gaccagaaag
    52681 gcacggattt tcagcatcgg gggaagctgc ctccttgtta tattatacct gggaatcgga
    52741 ttggccggtt tttgggttcc ttacattcag gcaaagcccc ccgcctatga ctttgcgttt
    52801 tttctctttg gcgctatcag cctctttttc cttcccgcaa tttttatatt cgtcaaggga
    52861 atgatagccg caaaacaacc catagaaatg atacttacag cacaatccat tggctttcga
    52921 ttccccggaa aatcatggca gacatggccg atagaacaaa tatcggcaat cagacttgaa
    52981 cccgtgaaaa taaatatttc tgtgaaaaat tctccggttt ttctttggtc tgaagcgaca
    53041 gcataccgga taatcttgaa atccaacacg caacaactta tgattgagca agatcgcgcc
    53101 cgacagtttg gctttcaacc cgaacaatta tacagagtgt ttcaacaact ctatcacggt
    53161 tgaaacagcg taatgatact ggaaaaatag atcaacatgg atagaggaat agaatggtat
    53221 tagatcttct ctttcgagaa aaagcaaaaa gtggatagag aggtttgcat tctggaccag
    53281 atatattcca ccgactacct catcacattc attgcagagt tccctcgaaa accgctgtac
    53341 ctctgccatc ccctctacta aacttaccgt tactttcgct ccagccttat aacccgcata
    53401 agcaaaacgc ggctaagtca gcgctagcca tgtcagactc gaaccgctgg ttagacttat
    53461 ccctcataca tttttgaaga ttatcctgat gttcttgatg atttgttaag atgaaaacaa
    53521 acagcctgcc tgtttttgtt catttctgct cataatctct aaagccaatt atgcaatatg
    53581 tcgaataata aatattattt actaaatttt atatattatc atttcttgac ttgccttcac
    53641 aacaaacaca actccaacaa tacgatccgg ctgtctatac gcagcgcagc ccgaatatcc
    53701 tcaaagctat cttgaaaaat cactttcaaa gcttttctga taaatacagc gaaaaatacg
    53761 atacagaata cggaaaaatt cgtcttgaac gaatagactc ttttaaggac agtttgatct
    53821 cttgcggcga ttacactaaa ggaatagccc gcatacaatg ttcaaatccc gaatgcaagc
    53881 atgaaatatt caggcctttt tcctgcaagc ggttctactt ttgcccctca tgcagtcaga
    53941 aaagagctat tcttttcgga gagcatattt caaatgaagt attatttaag cttccgcaca
    54001 ggcatttcgt tttcgcgttt ccgaaaatgc tgaggttatt tttcagaaac aacaagaaag
    54061 tgctttcaga tatagctcga ctgatctgtg aaatgataat agaatattat aaaatgctca
    54121 caggcaaaga aatacgcaca ggcatagttt tggcattcca gagctatggc gacttcttaa
    54181 gatacaatcc gcactttcac gctcttatca tggaaggagg attggcgaag aatttattat
    54241 catggaaaca cagcggattc agcatagaca attcgatcag ggtagcgaca tcggataaca
    54301 aggcaaggga agctattgcg caatatatgg caagatgtcc gatctctctg aataagatca
    54361 tctatgagcc gtttaaaggc aaagtgattt tcaagacaaa gtacaataag tatttcaaag
    54421 agaatcataa agtatatgat gctgatgagt ttattgccaa gcttgtgcag cacatacctt
    54481 tgccaaggat aaggctgata cgctattttg atctatattc ttccaaaagc agagggaagt
    54541 ggaaagactg ggatcatgtt tccaaacttg ctcctgacgg ctggaaagaa cagaatggaa
    54601 tagggattca gggagaagaa attgaaaaaa ccggataaga aggaattacc ggacctgcag
    54661 ccaaacctgt agatatttcc gcaaagaaaa gcaaatccac gtgggcaagg cttatagcta
    54721 aaatttatga agtggatcct ttagtctgca caaagtgcgg aagtgagatg aagatattgg
    54781 ctgttattac ggactcttat gagataaaca aaatactaaa acatcttatg aagataaaca
    54841 aagctccgac tggagttgag gagaaatatt tacttgatgt ataacttgat gtataacatt
    54901 ttatactttg attttttacc ctcgccaggg aaaggaaaag ttatgctctg gcaataaaaa
    54961 atacgcttat ttttttggct gattttattg cttttcgtga aaaatgcccg tcttactaaa
    55021 atatttcaca caaattaaat tattttttct ttctgaatga agactacgca tgattttttt
    55081 gaaaaactat ggaaattgag ttgactttcc tgaaaacccg cctatcgtcg ttcctatcaa
    55141 tcgtacattg acagtaatcc gagagacggt tattctatat cgtacgccca taccgggcaa
    55201 atactcattt aaaggagaag tccagtgaaa aagaatcgaa cggctttagg cgtcatcgcc
    55261 tcgatctgcc tcatcgtacc ggtgttgtcg ggatgcggac cgaagatcat caccgtgtcc
    55321 cttcgcgaca atatcaaatt tgccgagcgc actccgaaga tagcgttgcc gatcgggctc
    55381 tacgtgagcc ccgaggcccg cgagtatgtc atcaaatacc agctggacgg ccccctcggc
    55441 aaggtcaacg agttccgcca catgccgatc ggcgccgcgc tgatgccgaa cggcctgaga
    55501 tccctgtcga aggtctttca gactgtgcag cccatggact ccccggcgaa tgcccccgcc
    55561 tactatgtgg agctcaaggt cgacccggcc tcgagcctgg agatggggcg gtggaagttt
    55621 tccgagaaga gcgtggacct gcacctggtg tgcatggtga aacgcggcaa cggcgcgctc
    55681 atctggaaga aaaccatgaa ctcaaaagca gccaagtcga atcctcaggg atggttggcc
    55741 ggggtgtgga tgcccgccgc gataaagagc tcaaacacga agctcaaggc ggcgggagag
    55801 gagagcctct atctttgcct cgaagcgttg aacgccgagc tgataaagaa cagggcggcg
    55861 atattcagat agagcgttca ttgccgcacg gccgggcgca tcattaagat atgatgcgcg
    55921 ccggtcttgt tgtggcgccc ggcgtatcga agcggggaga gatcaaaaaa aggagcactt
    55981 actcaacgag ggatacgagc tgggttagtg gaccgctatg gacattggat gacaaccctg
    56041 tggctattgg taccatcgca ctccgcttca gccttttagc cggtaaacac gtggagaatc
    56101 accgacgtaa ttttcaagta tctcccggat attcatacaa cacgttttat gatgattctc
    56161 cgccgattat tttttataat ccttcagccc aatccccggt gcgagatacc tcttccagta
    56221 tgaggtgagg gaggtgcggg taaaaatagg tcactctttt cacagccagt gccctggcgt
    56281 aggtggttgc ctttcaacat gattatcaaa ggaactcata cgaaactgag tattccgcgt
    56341 tcaatagaaa aatttcccga actctacgga gcacttatgc aacagatcac cagaaaaccc
    56401 agcgttcctg atatcaccag gaaattcgat gctccttcat tcccctatca tttagcgttg
    56461 accagaaagg cacggatttt cagcatcggg ggaagctgcc tccttgttat attatacctg
    56521 ggaatcggat tggccggttt ttgggttcct tacattcagg caaagccccc cgcctatgac
    56581 tttgcgtttt ttctctttgg cgctatcagc ctctttttcc ttcccgcaat ttttatattc
    56641 gtcaagggaa tgatagccgc aaaacaaccc atagaaatga tacttacagc acaatccatt
    56701 ggctttcgat tccccggaaa atcatggcag acatggccga tagaacaaat atcggcaatc
    56761 agacttgaac ccgtgaaaat aaatatttct gtgaaaaatt ctccggtttt tctttggtct
    56821 gaagcgacag cataccggat aatcttgaaa tccaacacgc aacaacttat gattgagcaa
    56881 gatcgcgccc gacagtttgg ctttcaaccc gaacaattat acagagtgtt tcaacaactc
    56941 tatcacggtt gaaacagaga aacatggggt cagagcctat cataaggatt aggctcatct
    57001 ttatggttat aatacaatgc attccgagat atgaaaacga ataatcgttt ctcagcagga
    57061 agaaataacg gataaattcc acaagggaaa tataatatcc agagctatcg accatgccag
    57121 gctcaagcac tttccactga tttattacaa tttttatttg acacattccc tgccgctatc
    57181 acaatgatga aacaccagct catcatgttt attgttgtca aattacttat actgtgatac
    57241 atactattac tatgataaaa attggaacac ggggcagtga tttagcgtta tggcaggcgc
    57301 aatttgtggc aggcaagctt gcatgtgact atgagatagt aactatcaaa acaaagggtg
    57361 atcgtgtaca caatgtgtca tttgataaaa tggaaggcaa aggttttttt accaaagagt
    57421 tagaggatgc actgcttgaa ggcaggattg atatggcggt acactccatg aaggat
//
LOCUS       DAOOJH010000008        56191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_659_length_56191_cov_9.624501, whole genome shotgun sequence.
ACCESSION   DAOOJH010000008 DAOOJH010000000
VERSION     DAOOJH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 56191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56191
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_56191_cov_9.624501"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PLJ75_02135"
     CDS             <1..474
                     /locus_tag="PLJ75_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPD04050.1"
                     /translation="MILTDYLNKKNIIINPSVKDRWELIEAMLDMAIKNKSLKAEHRD
                     AIKKTLFEREKSMSTGIGKGVAIPHCSCPYVDDVVMIMSLSQKGINFDSIDNLPVHIV
                     ILLIVPKDKISQHIKTLANIAKLMNDDSLRESLIGAKDVQDIIKILKEYEKKGRK"
     gene            452..1495
                     /gene="ruvB"
                     /locus_tag="PLJ75_02140"
     CDS             452..1495
                     /gene="ruvB"
                     /locus_tag="PLJ75_02140"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPD04051.1"
                     /translation="MKKKEENNNPGIISPLSLDEEDFEKKLRPQRLSEFIGQKQLTEN
                     LRVFIESAKLRKKPLDHVLLAGPPGLGKTTLAFIVANELGVNIKATSAPAIEKSGDMA
                     SLLTNLEAMDVLFIDEIHRLSPAIEELLYSAMEDGELDIILGQGVGAKSIKLQLAPFT
                     LIGATTRTGLLTSALRDRFGIPLRLDYYEIDDLETIALRTAAIIGCSIEEEAAQEIAR
                     RSRRTPRIVNRLVKRVLDFAIVHKKDMIDKQIALYALDKLHIDMLGLDDMDRKILSTI
                     IDKYDGGPVGIKTIAISIGEEITTLEDFYEPYLVQIGMLKRTPRGRVAARLAYEHLGL
                     AYKGIEDIQTLFE"
     gene            complement(1497..2096)
                     /gene="yedF"
                     /locus_tag="PLJ75_02145"
     CDS             complement(1497..2096)
                     /gene="yedF"
                     /locus_tag="PLJ75_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPD04052.1"
                     /translation="MKTIDARGLECPQPVLLTKSAIEDENPDELEVLVSSQTSAGNVT
                     RFAEHSGYRVQTSIKDDYFTIVLHKAIEAKPFASAKLQSSMNNGKNVVVFITSNEFGR
                     GDTELGQLLMKSFINTMNEANVCPTTIIFAHSGIFLTTEDSPVIQPLQQIEKKGVQIL
                     NCGTCLEYYKRTKLLKVGTISNMYTITETLMMADKLIKL"
     gene            2259..5969
                     /locus_tag="PLJ75_02150"
     CDS             2259..5969
                     /locus_tag="PLJ75_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HPD04053.1"
                     /translation="MEKIFKRDFKYREIPYNYTSFSDKEIIVKYFDAHTWDLVEALRE
                     KRRTGRSAKLLFENIGDIFIIDRNPYIQFDIIENPTKLKNLQKRHQRRLATVKEGANG
                     DGQVLELIDKIEKLDDTAFKKLKECKKQQEKILNKLKHITSEKNIQFSPFHRAAHVTD
                     ATDWRVEYPFVVVYPDTVEEVQELVKAAKKLGLVIIARGGGTGLTGGAIPLLPNTMVI
                     NTEKFNRIGRIEKTYLNGNEVPVITVDAGAVTEDVIQYCEHQNYIFATDPTSAWACTI
                     GGNIAENAGGKKCVMWGTCIDNVLSFDMVDHNGELITVKRVKHLYRKIFPNDEVEFEV
                     IKNNKVVKTITLSGLDIRKKGLGKDITNKALGGLPGLQKEGCDGIIVSATFVLYKPFK
                     FTRTVCFEFFGNNMINASKAIVEIVKTFENDPVVFLTALEHFDEQYVKAIQYKNKSLR
                     TEIPKAVLVVDIESDEEEHLEKATLDLVAKVKQYNTEGIIAKDAGMREKFWQDRKNLS
                     AIARHTNAFKLNEDVVIPLERLQDFSDFIEKLNIQKELENNIQVIAVLADYLQERIKT
                     EDDKLCLERCQSGVAQLKTMQSRYMDILDNIDTHAKDYFKYDSEYALRMDSVFQLLQN
                     NELSINFDADVIEPLSKLFYGYDDILNTIYQLQADTQKRRIIVATHMHAGDGNIHVNI
                     PVHSNDYLMMKDAEETAANVMQATVTFGGVVSGEHGIGLTKIRFIDDDTLAKFAEYNR
                     LADPEDLFNPLKLTRDFNLETVYTPSFNLLEGEAFILKATDLETVFNSIATCIRCGKC
                     KSVCNTHYPDGMVFYNPRNKILATALVMEAVIYDIQTSTSLSFKHFTNLREISNYCTI
                     CHNCQKPCPVSIDFGAITLKIRSILEARRKSTFKPVTSFTLFYLKQKGYYINKLFRVL
                     LLRWVYSLQRFGYYIVKPIPHVVQFISPYIAMMLKGRLPKSGHKSLRDLLQLKNTNTF
                     YVFRNKNKPVIKTVVYFPGCGSERMFPDISIATVALLYYAGVRVIIPPTYLCCGYPMR
                     ANGKLNQARIKTNENRVLFHRMADTFSYLDIGDIVVSCGTCYEMLLDYQLENVFRGAR
                     LIDVNEFIAREGLYSKTINETLIYHEPCHTPLKAMGYQKTFSLLLNTKPLSVPNCCGE
                     GGTLALSTPDISNSLRERKETNIRANVVRKNVMVLTTCPSCVQGLCKIQDTIKVTGKS
                     LVVYIAEQCLGKEWKRQFLKEIKAQCFDRYIY"
     gene            6433..>6690
                     /locus_tag="PLJ75_02155"
     CDS             6433..>6690
                     /locus_tag="PLJ75_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPD04054.1"
                     /translation="MDALQPIKRVKLSESIVDMIIEKIKDGGFAPGTKLPPERDLMVQ
                     LGVGRSTLREAIQSLALMGILDVRPGEGTFIRLIRREDIISP"
     assembly_gap    6691..6790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6931..9801
                     /locus_tag="PLJ75_02160"
     CDS             6931..9801
                     /locus_tag="PLJ75_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04055.1"
                     /translation="MRYMYSIIFLIILFVSLPLQGYSAIDTFNNSNHDVTITPTPLVY
                     KKGTLLYRRVQVVFPSKETIQKLTIRTSVDGSISTMHTDNPDTSIFLDIKVPDLNTHK
                     AVFTMQCDTTTVKKKITIPAVKPWKIYCVPFTHVDIGFTQSQKNIRIQNYKNIDIELE
                     LVEKTKHYGKDASFKLFTEVSWAVQEYLSDATIEQDKKQKLIKALQKGDFELGAFLIS
                     HQNRFMSPDAIIASLQSTFNLAKKYNVTIHTACIHDVMDFSKVTKILCANDIPYLLVG
                     PNDTRYVLPPLFYGVSPDTSANVLVWHSAGLNGYGENFDLAMRLKLPFDEQAFTTMEN
                     SIALHLAQLEKGYPTAEIQQYFDYGNAQWDYPYDAYLLPYYPAQGGDNQPQNIVPSEI
                     AREWNKKMINPQIIIATPSQFFDYVRSRYNSVIPRIKGEMAPFWGEQIYLDFIQADPQ
                     RLLISYMFDRGMYQLASTVVDAMVHDKPINDSFHSIAESAYKAILLNNDHNPRPVPFG
                     KTHYTSDDVKDWMNTRSEWVKLPQILMHKAGYSEAFHDGRSWEKIKYKKNIIIIDSHF
                     YRVTIDQKKGRITGVYDKELQKEWIRNNAQYGFNQYVQTVRGENGARRDYYQCIEGFK
                     SPDVLVYRYHDDYRLIIEGTVKEYYKGMELIADFLDKSFHVSLPPWLLKVVYFFYQLF
                     TPSLTLTQEIVLPADEKKIELIQTFSGSQPQIAEHCFAYPLTSDALYYDAAFTPLLWE
                     SIYNGGNIIPAAKNTAPFKSINDTLYPFQWMYGLPPSFHCDAFTMVRDDAMYAVFMTK
                     DSKAIVPESSYSRDSNGFYHCCIGWTLWGKVGLGQSLKDKTVFRSTFTTFSADSITYA
                     LAKAYDKSFQEYGYTTSSPVTVSNPAIAVVWMYPITNKKFTVGLFETAGQSQSTHISI
                     KSIQPIAVYYSTCYGERKGDVADKQLLSLKPHELRFVTIEIQ"
     gene            9815..10519
                     /locus_tag="PLJ75_02165"
     CDS             9815..10519
                     /locus_tag="PLJ75_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HPD04056.1"
                     /translation="MAPAYTHEQIIELCKRLEKTYTPAVCDTLDEMGFMHQAMCSGFS
                     PIMPEAAIAGPAFTMEEAKTRKSTRLNEYDPEFVAKVLEDLFGSMQKGQVAVVNVNEF
                     YEAGAWGELMSTTCKYYGGVKGAVVDGPIRDINRVIEVEFPVWARGNIPTDSVGRVDL
                     VGIGGPIWCGGVRVNPGDIIFADCDGVVVIPINDVDLKEVVEKAEEVVTAERRSREEI
                     RGGKSLVEIYKKYGKL"
     gene            10537..11517
                     /locus_tag="PLJ75_02170"
     CDS             10537..11517
                     /locus_tag="PLJ75_02170"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD04057.1"
                     /translation="MSAICVTGCSGYLGRQVVKTLLEKNFAVTGIDLKDMNEPSVISN
                     KKFTFIRGDIVQKDTVHKACKGVQAIVHCAAALAQFVRDEKDMFAINVTGTKNVVEVC
                     KELKIPQLVFISSVEVYGIDVPVPCSEDVPLQPVCPYGRHKVEGERMCTQLAEKGYGV
                     SIVRPPTINGPGQNEPFLVAQMEAVYKGSPVILPGGGKTKLQMVDVRDVANAIYLCLE
                     KIKKGIIIANVSSDNVPTTRQLVEALFKHAGKKPKIISVPAWIARTIVKGLSAIKLSP
                     IEPQHLEIALRDYVFDNTRAKKLLGWQPEKSDIESTIDAYEYHINMRGWV"
     gene            11514..12215
                     /locus_tag="PLJ75_02175"
     CDS             11514..12215
                     /locus_tag="PLJ75_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD04058.1"
                     /translation="MKIEFTLIGQATVCINLDGIVVMTDPWWGSFEFLRAVPLTVDPE
                     SIMPVHYMLVSHNHVDHWCKRAIDVAVKRNIPIIGSTSAVKRAVKHNATKVKAMTPGQ
                     SVECEGFTVIAVPAFHPMAKDAVGFIIKKDSMVLYFSGDTLYTEELKKALTPYTITIA
                     MVQVACSTYPIIGKDGMDLQQAAQFVRECKPTIVIPIHYQVKGKHLVDETLQQWDVEA
                     QKVILPHGKGIAVTV"
     gene            12279..>12589
                     /locus_tag="PLJ75_02180"
     CDS             12279..>12589
                     /locus_tag="PLJ75_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04059.1"
                     /translation="MIENPPKGKQLLKVLGPGLVWSASAIGVSELVFATRAGALFGYL
                     LLWAPIVSLIMKYAIFEMAGRYTIATGENVLIAFTRVELDFKLFKLKTGWIIVLFWIV
                     F"
     assembly_gap    12590..12689
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12690..13181
                     /locus_tag="PLJ75_02185"
     CDS             <12690..13181
                     /locus_tag="PLJ75_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04060.1"
                     /translation="KGMVPQGEKLALQISQIAGENFGRIGEIIFLVGIFGTLFSTLLA
                     NIDGLCRVASNSVWYQRRKKDDTKLHYYRLFVIIYVLLTALFSVLFPAPVILLQISAV
                     IDTIFLPLIIGLGIYICHKHLEAIYRPGKLLTITGVLSVLFFVFFIVLFFYAVIMKVD
                     FSM"
     gene            13255..>13851
                     /locus_tag="PLJ75_02190"
     CDS             13255..>13851
                     /locus_tag="PLJ75_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPD04061.1"
                     /translation="MMGKEHTITSQFIAWGEILLRLSPPGYSRLFQTNMFEAYFGGAE
                     ANCAVTLSQVGIPCRIVSALPDTVLGDAAKRYLQGFGVITDNIVMKQDTRMGLYFYEQ
                     GAGLRASRLIYDRNNSAFAQLQHEDINWNAIIPSNAWFHTTGITLALSNTICDESIKA
                     ASIVNEKKGVVSFDINYRQTLWKSVDDARDAFLRLMPSI"
     assembly_gap    13852..13951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14201..14581
                     /locus_tag="PLJ75_02195"
     CDS             14201..14581
                     /locus_tag="PLJ75_02195"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HPD04062.1"
                     /translation="MMLQVLDIFFLLFHTLFTLFNMTGWIFARTRKIHLVTMLLTAFS
                     WCVLGIWYGWGYCFCTDWHWQVRNALRKPIVYNSYIQFLLHEITGYTPDAHITDITVA
                     GVFIICLCFSVILNIKDYTAKRKA"
     gene            complement(14585..15568)
                     /locus_tag="PLJ75_02200"
     CDS             complement(14585..15568)
                     /locus_tag="PLJ75_02200"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019534816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HPD04063.1"
                     /translation="MIKIAELLPPHPSALWRMVKQCGIEHVVGGMQLYAGWETMPQDF
                     WPWSYNSLVHIKTTYEDAGFKLEVIESRPPMDAIKLGLPERDREIENVIMLIKNMGAL
                     GIPVWCYEWMPVFGWTRTSTTTPIRGGALATSYNHEYMEHAPLTEYGIITEEQLWKNL
                     EYFLKAVIPEAEKAHVKLAMHPDDPPVSPIRGVARIMRSIDNFQKLLDLYPSPVNGIA
                     LCQGNFALMTDNLPQVIHHFGKQGKIFFVHFRNVKGTPYKFTETFHDDGMLDMYQCMK
                     AYHDTGFDGVMRPDHVPTMEGDSNENPAYSSIGRLFAIGYMKGLLEAVSKA"
     gene            15786..16538
                     /locus_tag="PLJ75_02205"
     CDS             15786..16538
                     /locus_tag="PLJ75_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HPD04064.1"
                     /translation="MKLIETLETKLDELIHIHGLVALKGGTEVEDMTHNELLFMKEIA
                     INIPVIVKIGGPEARNDIRYCQSIGIDGILAPMVESEYSLENFVSAMMDIYSNNTLPY
                     LAINVETITAYNNLPAIYSNPYFLYVNQVTVGRSDLSQSMKKAVDDNDVINATAHIVQ
                     CSKIAGKVTSVGGQINPHNAELVQKAIQPDRINTRHLVFDCAKTHNIATSIMVGLEFE
                     LDLYRAFSNVEPLKSTIYQKRIAVTTDRMAIV"
     gene            16665..16811
                     /locus_tag="PLJ75_02210"
     CDS             16665..16811
                     /locus_tag="PLJ75_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04065.1"
                     /translation="MHSIFVTIMVISSIIVLLIISKIGKNEAPGDVNNNWDGVWKERL
                     KIDN"
     gene            complement(16808..17440)
                     /locus_tag="PLJ75_02215"
     CDS             complement(16808..17440)
                     /locus_tag="PLJ75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPD04066.1"
                     /translation="MVKLDNPYSQYRPTWKVFIPMLVMYPLLKVAIQYAYFSFLYQYS
                     IVLLLLIVIAQYCDKGLFTKERIFAFDLPFERKIFYLMVMVIILIPIRNIAIQFTQNS
                     QTLHFYQGQMSSLESMLVMLAIIAPCEELFFRGFVQYNLTFYFGKYGAIVIGALFYSL
                     VGIAAHSLYLAVIFFLIGITIGYIYSTVKSLLQSIVLHVITIIFMYIYPF"
     gene            17605..18579
                     /locus_tag="PLJ75_02220"
     CDS             17605..18579
                     /locus_tag="PLJ75_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04067.1"
                     /translation="MRRIIVIVCLLHTSLAYPQLLINEIACNVPAGDWVELFYCSEQR
                     LSMDISSLYVTMYYGTNEPLSQSPVTIYSYDRAETPWDDRFVVVHCTKPGIPDECDAT
                     GDTNGNGVLDVYCNNYINSLWNTDCVVAIDSDDEPSNGGIIDFVAYANRDGTLNQSIE
                     KYLKSAIDHNQWVGAYASEECMVDIGQKGILEYQSIIRKNGVDTNSADDFVITSYQTP
                     GRPNILPINHSKSEFIKIKRTIVISKKNMQPLNLFAIQPCKFRIRLFSSVGYKIYESP
                     LINALPGVQLLDWTNTIKHLTCGLYLGIAEAQDNANRESKKFYCIVVE"
     gene            18579..19820
                     /locus_tag="PLJ75_02225"
     CDS             18579..19820
                     /locus_tag="PLJ75_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04068.1"
                     /translation="MGSLLLTITMFIGAFENAYKVALPLHVQDPLLGKLYNNYPAFVC
                     SLSYANPFGFPQLKETNTNVAYKGKKYDGGIRWHYFGIPEYREDTLSTGLDWAIAHWI
                     KVGCRVHDYILSIKTQEFTTTKHSVDYGLLATVTPFQRLSCTLFTNNIQHYGDDEYIT
                     PTTIFSCDADIFDGCGIEYTLLYNGDIQQCIWIKGYITRHVSVLVGYSKELELSTAGV
                     NVAWGNILLYYMLQHHSFLGNTHRFGITYAGAGYVFPAHSEYKQNTAIKLNIQTCSQQ
                     ELIALGIAPDVLCQRIIKYREMFGAVSTRSLYQLGFTTQQIRQLQEVTCNYCDDDKDE
                     KKDYHKKYQGRTYISKEEQNKKIKRLFQSMVAADIPSDVAIVLAEEYQKAGIPGILRS
                     PLYRSLDTYQQKMVRAACGIQ"
     gene            19805..21361
                     /locus_tag="PLJ75_02230"
     CDS             19805..21361
                     /locus_tag="PLJ75_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04069.1"
                     /translation="MRNTIVVVLSMVYTVVFIHAVMALSTISVEYREDAMDYEADTQR
                     YLRSSISVTTDYAKVYATLIKTNNGKTTATWAFSNTGEYAAIVAGYYFIHNASGLLLG
                     KPAVYNPDPYSFPVRSSETGISLCKSGNPDYAMHGIVASLYDRLNKPQVRLRIGASLQ
                     ERFMRSDDVEGKLYPYSIGGLLSHDSRDGLYSEPVNIITYFANVTLQPFEYVVLQGCY
                     YHTGLYHENTKIVFDKDNENATAIQSFGGYSLYGRYQDNILTVFTEWAISQKDVKGNS
                     TSTSQYNNAHLYGVTVKKKGIQLCSIIQKTERYFYAPFSSSFGGNAPRDIYYYSAQIK
                     PIRWMLLSWTYMDQNYLVPSTYYAEYPHKRLHCIRTQFTINAFKIQTDYRFMTFYKAG
                     AEQRDSRYQTGMEWMVAKKVSLNMKYGLYTGEESAWYMRSGYTIQWGSLQQAMSVVYV
                     NSNGRKVYCAMLPLANSNILTEVCSSTAVYLAMRVGYRSKLCQLSLRLLSELSHIKNA
                     KGEFGATAWF"
     gene            21421..22224
                     /gene="larE"
                     /locus_tag="PLJ75_02235"
     CDS             21421..22224
                     /gene="larE"
                     /locus_tag="PLJ75_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPD04070.1"
                     /translation="MDEKKIEQVHQILKKLQPYIVAYSGGNDSTLLVAISMRWNLDFC
                     AVHCISEFTIPGETEYARYFAQHHRIPYHEITVSVMNNSQIIANTEMRCYHCKHEIFK
                     NMVAYAHANGFINVIDASNASDSNDYRPGKKALAELGILSPFVEAGITKDDIYSALAD
                     IQIPHKQFSNSCLATRIPYHTSITNIMLDSIAKAETFIAMLGFTAVRVRYHYPIARIE
                     IPEEEIAYFMDATLRNKVVAFIKELGFLYVTVDCEGFSSGNLNRMLPHE"
     gene            22217..22897
                     /locus_tag="PLJ75_02240"
     CDS             22217..22897
                     /locus_tag="PLJ75_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04071.1"
                     /translation="MSDILAAFVARNTNEIQTNLDNIQECPFPNMMPLIINDGVEIES
                     ASELADTGIHFVIHEEPLGYGGALVSALSYARDFSLHYLFIMPVEAFTGWQAIATYVQ
                     HFETSDILTGNRLPSSCSAPEGFYGEIVTCLNNGTGLHIVDPLSPVKGIAMKHCDSFE
                     ITEFPDTAFLQLLIQARHFHISIKEFQCSYPESLITSQMQDVEDLPNIKDFIAGELYL
                     YPYIQKGH"
     gene            22897..23976
                     /locus_tag="PLJ75_02245"
     CDS             22897..23976
                     /locus_tag="PLJ75_02245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPD04072.1"
                     /translation="MTVQRIGLIIVLLLNVGLCCSAPEHRLPEQYSGLPSESSVLFVG
                     DMMFEWAVKDTMSKKGVDYPLQNIQSFLHSFDFVMANLETPLTNNSFGDSSFDKKYIF
                     KTTMDIAPLLHKAGIHAVTIANNHILDYGVGGLTDTLVALSSCNILYAGAGINETTAS
                     QPIFCKLGTMQMVIICYTQICSKDMIARDNPGVNFFELSKAIQDIQRYRFADFIVINL
                     HWGNEYFYYPSSKQIGIAHALIDAGADVIVGHHPHVYQGIEIYKNKPIIYSLGNFLFG
                     SMHEGINDTIACALYIAPGGKITRIRVYAIKGKFDDQIIQPEVLGADDATHLLHHFFQ
                     ISKPLDGNFVNRAYITNNYIEYNFR"
     gene            complement(23977..24918)
                     /locus_tag="PLJ75_02250"
     CDS             complement(23977..24918)
                     /locus_tag="PLJ75_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPD04073.1"
                     /translation="MSYTAYCFHPHYLNHTTGDSHPESPKRLEQLDKYIRNNNNYSSL
                     LYVQPRKADPKVLTYAHDINYIQSIANAKPNGITCIDGYDTVICDETYDVSLWAVGGI
                     LEVCDYIMSGEAHNGFCAIRPPGHHAEIAQAMGFCIFNNVAIAARYLQYTYNIRKIAI
                     IDWDAHHGNGTQHIFEADPSVFYISLHQFPFYPGTGSVDEIGIGPGYGYTLNIPMNPG
                     SNDKTYKSAFTEIIIPALDHFQPEFILISAGFDAHKSDPLSSLNLSSSAYYMFTRMLL
                     DIAHKYSNKRVIVVLEGGYEINSMVESVDFVLKALME"
     gene            complement(25014..>25214)
                     /locus_tag="PLJ75_02255"
     CDS             complement(25014..>25214)
                     /locus_tag="PLJ75_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083777221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPD04074.1"
                     /translation="YEVLTPPVGRNVLETIRQIHAFQLVRESKGTQATPSGWRPGKDT
                     LKPAPDLVGKVWEVWSVDKAFD"
     assembly_gap    25215..25314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25397..25816)
                     /locus_tag="PLJ75_02260"
     CDS             complement(25397..25816)
                     /locus_tag="PLJ75_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008085364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPD04075.1"
                     /translation="MATITEYITIATKGQGDIIDITMDAQAIVTNNTINNGLLCLFVP
                     GSTAAITTIEYEPGLQKDINIFLETIIPRNAHYNHHDTWHDDNGSSHLRAAMIGASIT
                     VPIVQGKLTLGTWQQIVLIECDTRKRDRKIVAQIVYS"
     gene            complement(25824..26315)
                     /locus_tag="PLJ75_02265"
     CDS             complement(25824..26315)
                     /locus_tag="PLJ75_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04076.1"
                     /translation="MKKLSPKSQKWLKAFHILCVSLWVGGAITVSGKQFFIQAKTDGE
                     LYGIVSTMDYVDLFIIIPGALGCFVTGLMYSTLTPWGWFKYRWIIVKWIICLYGIIFG
                     TYFLGPWLGKLVHIAQTQGLASYDNPQFNSTVILLMIFGTFQAATLIVACFISSLKPW
                     GKR"
     gene            26817..27875
                     /locus_tag="PLJ75_02270"
     CDS             26817..27875
                     /locus_tag="PLJ75_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPD04077.1"
                     /translation="MLERLLNIRLFNNTVKEYCVSLLIFIVGIIAIYIFKTIIIRLLK
                     SWARKTTTELDDIAVEAFEKKFIPLIYICSFYVSIIQLTLHPSLKKTLDVIVLILMTI
                     FGVMFVLNGIKIFISAIIQKEGNVTKQKALRALNPLIQILVWSFAIFILLENLGIKLT
                     PLIAGLGIGGLAVALAAQNILGDLFAYFVMLFDRPFEIEDFIIIDKDFMGTVEKIGIK
                     SSKIRGIGGEQIIISNTNLANARVRNYKRMNRRRVVFTIGVVYETGLEDLTLIPEIIK
                     EIIQNIDGTTFDRAHFFSYGDFSLNFEIVYYVNSNDYNVYMDIQQEINLKIKDEFEKH
                     NIEFAYPTSLIYVTKSKM"
     gene            27921..28067
                     /locus_tag="PLJ75_02275"
     CDS             27921..28067
                     /locus_tag="PLJ75_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04078.1"
                     /translation="MRLVIISNRLPVTVSEKNGRLIIKESVGGLSTGLKAYIDFIKNS
                     SSEN"
     gene            28572..30698
                     /locus_tag="PLJ75_02280"
     CDS             28572..30698
                     /locus_tag="PLJ75_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD04079.1"
                     /translation="MKTFFYPESMVVVGVSLTKINLGKIILLNNIRQGYKGILYGVST
                     HEGEIEGVIVYKDILDLPTTPDVAIIITPAKTVPEQLEKCGKKGICHVVIESGGFSEY
                     SEQEQSMEEQILGIAKKYGIQIIGPNCIGIINFEEKVIMPFAFIQNIPEAGQVSLISQ
                     SGGVGNTYLHALPENHIFMNKFISIGNKLMLDEVDFLNYLVNEDEATRIILCYLEGFN
                     RGKEFFEIARLSEKPIIVHKSNRSPISAKIAQSHTTALSASDAIVDAAFAQSGVIRAE
                     DEEEMIKAVKAMQLPLMKGRNVAVLSRSGGHAVISADACAKYNFNLVSFPQSYIDIIR
                     TIYNTRIIAHQNPLDLGEIFDYTIFGRIVEETIKLDNVDGILFNHLYQSEYEATMSRT
                     FLDTVERLIKQYNKPVMIAMISDAQEMLDINMNHPMPIFNTPYSAAHALAISAEYYQK
                     KQMRNNRGEIDYRKMEKVKQDFAVLNDVLCNNKNPLLHESISFCESIGLPIVNGALIT
                     TVDEIYEKEFQFPVVAKIVSPDATHKTEVDGVRLNIMDVEQLEDAFIDMKQSLYDYNP
                     NAVFKGIYVHTMAKAGVEFIVGAQKDAVFGPIVMVGLGGIYVELFKDIAIRLAPVTKV
                     EAWDMIQQLKSAQLFEGFRGKEPLDAEALSEVIMTLSYAISAIDEIADIECNPVMVYP
                     KGEGCIAVDARMVLQKQTIVSKATTS"
     gene            30813..32195
                     /locus_tag="PLJ75_02285"
     CDS             30813..32195
                     /locus_tag="PLJ75_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD04080.1"
                     /translation="MQRTVETLPLWKKIIYALGQLGWSLASFSVGNLLVYFYLPPEES
                     NNAIFQPYIYQGYVIGVLTIIGLIYAFGRLWDAITDPIIATMSDRSKSRFGRRRKFLA
                     IGGLPFALLSILAFTPPVNGMSPWNAVWLFVVVTLFYLFMTMYVTPFFAWLSELGHNP
                     RERLFLSTLISITWAIGFAIGSQAVALQSMFEKSYGYSSHRAFQTVMVIFGGISLILM
                     YLPVLFIDERRYCESNVSTEGTFEALRSAFKNKNFLFFTISDLAYWVALTFITTGLVY
                     YVTVLLQMDKAVYSQYMIILFALSFIFYVPVNVIAQKIGKKKLMIIAFILFSMGYVIA
                     SMLGWFPFDKNIQGLIVIIITSLPLAIFGILPNAIIADIAEADGIEKGNFKAGIFFGA
                     RTFMSKMGQMIAALVFPSLILLGKTPENDIGVRLTCIAACFFCIAGLLLFMKYDEKKI
                     ISILAKKEKV"
     gene            32572..33036
                     /locus_tag="PLJ75_02290"
     CDS             32572..33036
                     /locus_tag="PLJ75_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04081.1"
                     /translation="MIKVTISRTFSFPIDEVYSAIDHINKLARLKPAFRNYRIARDEE
                     GLQLIDVDIRFIVNKFPMRLTYTAIPFKYCELKKLKGAIKEYSYTYTFEQSDNKTSVT
                     ADCAIVLPLRYFIVQPFLSAIIKHRLAKELTILEKIVNQKFNGYDKTTYRSE"
     gene            33008..34690
                     /locus_tag="PLJ75_02295"
     CDS             33008..34690
                     /locus_tag="PLJ75_02295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD04082.1"
                     /translation="MIKPLIVQSDNTLLLEVDNPLYEEVRDAILPFAELEKSPEHIHT
                     YRITPLSLWNAASSGLTTKEILERIAQYSRYDVPSIVVTTIKEQMQRYGLLTLIKHNE
                     NCILQSNDAHIIREIVTHKSIQKFIDSQLSDYELVIKPYSRGHIKQALIKIGFPVEDK
                     AGYIDGDPCPFALRTVTQSGSPFVIRDYQQNAINSFYRHGSDEGGSGVVVLPCGAGKT
                     IVGMGIMHIYQTKTLILVTNTVALRQWIDELLDKTTLSTDMIGEYSGDVKDIKPVTIA
                     TYNILTYRRKKEDEFKHFKLFTENNWGLIIYDEVHLLPAPVFRMTAEIQSKRRLGLTA
                     TLVREDGLETDVFSLIGPKKFDMPWKMLEECNWIAKAICSEIRIELPESIRYQYTVAG
                     DRQKFRIASENEKKLYYIERLLNAHPNDRVLIIGQYIDQLQIIKERFNYPIITGSTPV
                     KEREELYKQFKGGTITRLIVSKVANFSIDLPDANVAIQVSGTFGSRQEEAQRLGRILR
                     PKPGDNTAYFYSIITAHTVEEHFAHNRQLFLTEQGYTYIIMNESMFHEKFSA"
     gene            34671..36476
                     /locus_tag="PLJ75_02300"
     CDS             34671..36476
                     /locus_tag="PLJ75_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPD04083.1"
                     /translation="MKSLALSLILVFSSTVSSWKPLLVQNNGFLYYHEAKCKGLPANI
                     VINDIQVHGNIIYLTTSQHGIFKNNGGDSYWENITPASAYTRTIHSPCNQYRSISAFC
                     VDGCDPARLYCATKYTLYTSGDAGKRWEVIPMTGLPANACITALYAKGGQLVVGTSWN
                     GIYRYTAGRFVAVNGGFPRKMYSDSIGFYETIAHVSGGSPVRAATRFTPQVYSYGASG
                     WKMVFAGNGGPYYGCSLYEDGWAIVDGDRLVVNNNGTFVYQELSPKNFCCAVLTDGTH
                     YIFVKAVKTSNDSIRGLYAATPTSWQTIKKCVGYAKRSGCNALVFDIKDDYGNVYGIN
                     NETAAMIGALQTSLPLHELCNYCHANGIQAIARMVVFKDKRLFSGFKHRYAIVDAATK
                     KAWQGNPKEYWIDPYSDFVHRYTIAIAQQAQDAGFDEIQFDYVRFPTDGPVNRCVFTF
                     KPDNGIYKYEAITDFLRTAKASLAVPVSVDVYGFTVWYEFGQWLGQDIEAMTGIIDVL
                     CPMVYPSHYGRKFFDGKITPEGYYTIVRESLSRAYMYSKATVRPYLQAFNMLSPGWGY
                     DYIQKQIQACTDTGTQGYIFWNAGRDYEVLINKNK"
     gene            36875..37168
                     /locus_tag="PLJ75_02305"
     CDS             36875..37168
                     /locus_tag="PLJ75_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04084.1"
                     /translation="MRISEGLSLFSLPSAVSSQPKSTMMVWPVYDDTRVTPIKGIARR
                     LENNVQYVKPTPDEHARLLSQIHDNKEPLYTNNAFIQKNMQYTIPGTFFDAIA"
     gene            37289..37774
                     /locus_tag="PLJ75_02310"
     CDS             37289..37774
                     /locus_tag="PLJ75_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04085.1"
                     /translation="MDFETTTCISYEHLNTLYSLADKFNMPLHSCIVYLLIYAAKKEK
                     APAKAFKGISYRSRNKLNPWKRVHLYLAFKEYEYLLDVKKIWKMSVARAIAFCVENVL
                     DEFVQFLNNLIEAEREGDIDNYLVYEINRSYLFEYDTKEGVHCCRFYWGLPRKYARCK
                     P"
     gene            38033..38635
                     /locus_tag="PLJ75_02315"
     CDS             38033..38635
                     /locus_tag="PLJ75_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04086.1"
                     /translation="MVKKLSLCIMALCMACMTTGLLAQELTDDVAFLRVGYQWNTVNF
                     DKNLPVIGDNLENNGIAIEGEYNLNLGGWLLGVSLEWARLVNDDDSDFVFYYLSPMIS
                     LKFIAAGGLYIGPGISARYMMSADTPSGVDEPDKEVDLWVNAVAGWMTPIAEAVYLDV
                     QARFGWNLTKNQFEDVGDIDNNYDYAVYVGIGTRTRSTGI"
     gene            complement(38709..40766)
                     /locus_tag="PLJ75_02320"
     CDS             complement(38709..40766)
                     /locus_tag="PLJ75_02320"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD04087.1"
                     /translation="MNNTTLVSVTIAMIFSCSSLAYSAEATYDMRNLKFDTTTTYAIK
                     AQWLFWWNQIIDPVSIDDNKAPQPTDIVMMPQHWTDYKLNGTLLPAKGCATYALKILL
                     PDNAIYGIYLDHMVTAYRLYINGVEVANNGKVSKNLSAMRNECRHQAIYFTPKNKTAL
                     CVLQISNRDYRTAGMWHPLIVGKDRAILAHYNTKVISDLFLFGAILIIGLYNIALFLF
                     RTRDRAPLWFGLFCLTVCARIAATGSTTIANIFPGFPWELQIKMELSVFYLGSLFFGL
                     FFHEIYPMEIKKKHLQFFNGIFILLTVLAITTPVSFFNRLVPYMQIAVMAGLSYILVC
                     LIIAALRKRPHIHYFLAGFVLFMVTAVNDILYAKNILPTMYILPAGLFVFIFTQAIAI
                     ARIFSFSFLQVEHLSAHLSDLNISLERFVPHEFLSFLQKQSILDVQLGDQTLKDITIL
                     FADIRSFTTLSEKMSPEETFKFLNSYLNRIGPIIRSHNGFIDKYIGDGIMALFPQSPQ
                     DALNAAIAMVKRIAEYNDERKKYQLSPISIGIGIHTGSAILGIIGETMRIESTVISDT
                     VNLSSRIESLTKHFRTTILVSGDVYEQVKDIPEYNFRYISKVIVKGKSQPTKVYEVLS
                     MYPDDAVALFTSTKEQFKKALTLYNKKQYTEALHIFKEIALKNPCDMITKNFIEDMEE
                     KKD"
     gene            complement(40949..41941)
                     /locus_tag="PLJ75_02325"
     CDS             complement(40949..41941)
                     /locus_tag="PLJ75_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPD04088.1"
                     /translation="MDNTEKPLITISFNLNAEEKDRIESVLSPIARIIYIKDLNADER
                     AKLLQTSHVVLAWAPHSEFSPAEITLLEKVKFMQLLSAGADHLDFSRLPANLTVASNV
                     GAYAQPMAEHVLAMVLALSKRLYIHHEKLKQGIFDQFSENRALSGTTAAILGFGGIGR
                     EVARLFKPFNMHILAINRSGKSPDPAEFLGTPDDMGKVLPLADIIVITLPLNRATRGL
                     IGEREFKLMKPDSILVNVGRGEIIDEGAFYHHLKNTPTFMAGIDAWWTEPFRHGHFEM
                     KYPFLDLPNVLGTPHNSALVHGALATAAQKAAQNIRRFLLSEAITGLVRPEDYE"
     gene            42493..43311
                     /locus_tag="PLJ75_02330"
     CDS             42493..43311
                     /locus_tag="PLJ75_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPD04089.1"
                     /translation="MEYKYYLVEKKPPVAWVFLNRPEKKNAMNPAAWWESPSVFKELD
                     ADDDIRVIVLAAKGASFTVGIDLIEMAGEIKELAEPSQKGGIKRSLLQKIYRMQDTMT
                     CIEKCRKPVIAAVHGYCIGAGLDMIASCDIRLASADAQFSLREAAVGFVADVGVLQRL
                     PHIVGQGITRELAYTAKYITAQRAKEISLINEVFADQKALYAGAEKMAMEIAENSPLA
                     VQASKDVLNFGIGKTTDEALKYVASISANIIPSDDLFEAFTAFAQKRKPNFTGK"
     gene            complement(43375..44805)
                     /locus_tag="PLJ75_02335"
     CDS             complement(43375..44805)
                     /locus_tag="PLJ75_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HPD04090.1"
                     /translation="MKRFLKWLAIVIGGLIALVIIASIVLMLIINKDMIAQQAEKVLN
                     RHVTIESIDVSIFSVLSGIEVKGVAISNFKTPQQLSALKGKPVDKADLFAGLDSFTFK
                     LKILPLLQGKFELRELLLSGPKVNIVRYKSGAFNFSDLMKPSTKEEKKVEEVKKEEPS
                     KPLTADTLPVSITIGRIGIEKGTVTFTDQSSGQKVMLYNCNALVHNIEIDPKDLKNKN
                     QVKLTVDIGIKTIGKTSTGSVESFDIGLSAKGNVKPFDLKTRILNPEIALKAGSPYGT
                     MTGLQIFDAIKSNETLANYAGKFDFLAKDVKWKNGYIDIWYKGGLLKITNGKISTDDY
                     AGNFKADINLNTSTVNADIDMILNEKHNKSIYNGIKGNASKLIKGDVAKYVKPENVAD
                     QAFKYLTNKDGKVYLKFAVTGPMNKPKTKLVEPKLPSLNDIIKDMAGDVVDVAKEKAV
                     KKVSQTVEKTTDKAKDKATKKLKKLF"
     gene            45175..46533
                     /locus_tag="PLJ75_02340"
     CDS             45175..46533
                     /locus_tag="PLJ75_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPD04091.1"
                     /translation="MKSKIIITVSFVLCVGYALFLGCKSTPHYRLAQFISPSTCGGCH
                     DEIYEQWKNSMHNLSQKDVIYKEFARFYLDDSKSQNPVNNDELKEAESCVKCHIPVGY
                     ITGKPVSVTEELSMEKDLPEITMEGIQCDYCHTIVEAKKYYNARFVYKPGNGEADPGI
                     KRGPFKDANPDWHTAEYSSFHTKASFCGTCHDVRHVVYGTKLETTFEEWTKSPYNTGD
                     SKTTVPCQGCHMYHRPGVPATGSTPRPDNPGFAAFGGPQRDHIFTHYFVGGNSVIPSL
                     YKDTVKAQMAQERLKNAAVVEIETSDSNSGTVGIAVTNIGAGHKLPTGLTDVRQMWLE
                     IVVVNQYGREVFAIGKADKKGYLPDNTIIFKTVFGDGSGKSVMNIAKAREILFDNRIP
                     PKQKVTSTIMLPKGLKGNYTIKVRLLYRIADQKAVDTVMKENAVKLDIIEMATATKNI
                     IL"
     gene            46540..47136
                     /locus_tag="PLJ75_02345"
     CDS             46540..47136
                     /locus_tag="PLJ75_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04092.1"
                     /translation="MVTVKASNLSKRYTRILFRDMSFCIATGQSLAIAGPNGSGKSTL
                     LKIVAGVMTPDSGNVEYWYNDKPVDTAYIRNYIGFAAPYINVYKYLTALENIAFAIGA
                     VDDDAVQLLDLLGLSPHSDTKLMFYSTGMIQRVKLALAFLKKASVLCLDEPGSNLDEQ
                     GKSVLFSLLEKVQPATAIVIATNDVHELNFCQRSIVLD"
     gene            47129..47791
                     /locus_tag="PLJ75_02350"
     CDS             47129..47791
                     /locus_tag="PLJ75_02350"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HPD04093.1"
                     /translation="MIKALWRNIQKDILIEFRSGIIVSIVLSFSVIVTISIGFATGGI
                     INSPLLHAVILWIIIFFSGMNTLSHVFFREIEESTALFGVIHYPAAVIYLSKLLINCC
                     VMVVIAIMVIIIYTFVISVSVYYGVYLIAILIAGSLSLAFATTVIGAIAALAGARGGL
                     FTILAFPVVLPLFTVAIQSTARCFQQQVFSPLSAIIFLLAFSIVLGMVSYLLFPYIWR
                     EF"
     gene            47674..48456
                     /gene="ccsA"
                     /locus_tag="PLJ75_02355"
     CDS             47674..48456
                     /gene="ccsA"
                     /locus_tag="PLJ75_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPD04094.1"
                     /translation="MFSTAGFFSIKRYNFSTCIFNSSWNGIIFIISIYLARILIVTRI
                     AMIVILLNGIVMMLSLYLIYIWVPPAAILGETSRIVYLHVPLAWVSCLAFAVAGIASL
                     LFLKKSKINYIVYARSSAHIGLFFSILTVITGSLWAKVMWGSYWNWDPRETSITLLLL
                     LYIAYFSLQQIVSGNPKAPMLESVYLIIAFVVSPFLMFVAPRLYPTLHPEPVINAQGQ
                     LMLDTEMRLVLGISLVSFTLLYYILLGLWVRIHVLKSKLHEE"
     gene            48458..48631
                     /locus_tag="PLJ75_02360"
     CDS             48458..48631
                     /locus_tag="PLJ75_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HPD04095.1"
                     /translation="MKLYAIVTAMHFLAQTDSNYSKTLFWVSAVVWCGIVCYLVVLHR
                     KIKQLEKRVNGKK"
     gene            48618..48989
                     /locus_tag="PLJ75_02365"
     CDS             48618..48989
                     /locus_tag="PLJ75_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HPD04096.1"
                     /translation="MAKSKIIIVAIIVICSAVALFSFKGLLTPYVPFDDAKNNPDYVQ
                     IIGRLVKTVPMQNTTDGFLFVIEDNALERMTVLYRGAKPLNFEHATSVVAIGIYNNKK
                     QMFEADKILVKCPSKYTREQK"
     gene            48990..51146
                     /gene="ccsA"
                     /locus_tag="PLJ75_02370"
     CDS             48990..51146
                     /gene="ccsA"
                     /locus_tag="PLJ75_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPD04097.1"
                     /translation="MNSLYYLPWITALLIMLSTMAAVYSLSKPKIFTLAKYLYYAATG
                     IVALMLICLLMAFVTFKFQYSYVYHYSSVDLPVLYRIAGVWAGQEGSIVLWTFFIMIA
                     GVIAVTREEQLKQGFLTIVGLVSLIFVIHCINNNPFTYIWDTSKNFPVNIIPTDGAGL
                     NPLLQDFWMIIHPPVLFIGYALSTIAFIYAILSLLYNNYSIILTAYRYVLATVLTLGA
                     GIFLGGYWAYKVLGWGGYWGWDPVENASLIPWLISIALLHGLILQKRKQVLVRANIAM
                     AIGVYVFVLFGTFLTRSGVLSDFSVHSFNGAGNFTDLLLFLSTTVVLSIGLLIYRSRD
                     ARGKVLDNDVLSKENIMVYGIAAMVFFALFVIIGTCMPLLTRLLSQPSTVRESYYISI
                     VKPVGLVIVILLLLATFTKKLPLKARTFVIPITIALTLTILLYIGETIRIFPLVLMFV
                     SFFIIVTTIKGLFPYTHLNRAARVAHSGVALLIIGIITSQTQSLDYHTELIQKEKKTI
                     YPVSLELVGMTGGAKNALVFHYSYYNKGKTITTPYFIDTRTNQLFREPYIDYNILYDV
                     YISPVEYMSGYDKLTQVMLKKGEIRTIGDITIRFDNFDVDRAAMMKGNAIVYAQCTVT
                     HKGRQYTVKPGVAYTNDRRQDIKAMIPGTNRTIVLLDFDVRDAIVVLHIDAHDNAVLP
                     PDSVIVDISFKRLIWLVWLGTVIIVVGVALPFVRRS"
     gene            51171..51623
                     /gene="smpB"
                     /locus_tag="PLJ75_02375"
     CDS             51171..51623
                     /gene="smpB"
                     /locus_tag="PLJ75_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPD04098.1"
                     /translation="MSSETEIVQNKKARFEYEILDTIEAGIVLKGTEVKSVRLKKVNI
                     QDSYARIKDGEAFLYGMNISPYESGNIFNHDPLRVRKLLLHKQEIKRLTGKQQEKGLT
                     LVPLKVYWKNGKVKVLLGLAKGKTLYDKRHDIKKRDSDRELQRYKRMR"
     gene            complement(51655..53202)
                     /gene="guaA"
                     /locus_tag="PLJ75_02380"
     CDS             complement(51655..53202)
                     /gene="guaA"
                     /locus_tag="PLJ75_02380"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPD04099.1"
                     /translation="MSSQNKVLILDFGSQYTQLIARKIRELHVYAEIVPYTITIADIK
                     KENPGAIVLSGGPSSLYEDNAPHISDDLFSLGLPVLGICYGLYAVVNAFHGIINPSPF
                     KEYGRATITLIEESPLFKDLPRTQTVWMSHGDTVERIPETFKTIARSENAPHAAIENR
                     EKKIYGVQFHPEVYHTQYGKEILRNFLFTICNFQPTWTMQSFIDAAIDDIRTMVKNGT
                     VLLGLSGGVDSSVTAVLLQKAIGDRLYCVFVNNGLLRKDEHIKVIERFKKHLKLNLIY
                     ADAEEHFLHKLKGVIDPEEKRKIIGKEFITIFKQEAAKIGTFDFLAQGTLYPDVIESV
                     STKGPSDTIKSHHNRVPEVLELINEGKVIEPLKELFKDEVRELGRQLGMPEELINRHP
                     FPGPGLAIRIIGEVTRERLAIVREADDIFIEEIKNAHFYNTLWQAFAVFLPVKSVGVM
                     GDKRTYDNVIALRAVTSLDAMTADWAYLPEDLMRRISTRIINEVKGVNRVVYDISSKP
                     PSTIEWE"
     assembly_gap    53206..53305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            53953..55308
                     /locus_tag="PLJ75_02385"
     CDS             53953..55308
                     /locus_tag="PLJ75_02385"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04100.1"
                     /translation="MSLLSITFFIVGASIVGALLVIIRAHQYPAKVRKAQEYLDNGDI
                     AKASEIVKLVLERKKDYIPARYLRAQILIQQKQYLLAIAELNGILSIPDFDKYVEELN
                     IHYQLADLYHETQQWQKEVEEFRVILQFNPDDINANHRLGIVLYRQHDYTGAKDYLLK
                     AVTLDPALSDIYAPLGICLYNTGEYGTAENFLIKAVETNATNYEAHYYLGMIFKMKKD
                     YTAALEMLSKARNDRRYFDKSQYAIGQLYFDNDAYSKAIEELEKGLPYIKDNTDEGLA
                     YRYLLAECYEMENKIPEAVHHWEKIASINPNYRSTQVKLDDYKLILENKNLKFLFTST
                     MAELQPLITEIIAQLNYSIVYKKEVSANEYMYKCFSVKRINEPPVLINFIRTTRDITE
                     GQINTFYQRMLEEKCKSGIYITTSKFSINAKSQAANKMIEIMDGRFLQKAMEKIKGKK
                     G"
     gene            55311..>56191
                     /locus_tag="PLJ75_02390"
     CDS             55311..>56191
                     /locus_tag="PLJ75_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04101.1"
                     /translation="MILRYLSTCTIVLLLITLSCSRQKTVNEGIELYQEGLYQSAFIK
                     IKDGLIQYSMEKTLDEQSFVCTPYAVFSTETLLTMLYPKKHTYEFPREVVSVWYDPAD
                     DVCVVTDGIMVSNFSSDEEIIINNPTTKPIKAVSYCNKDIYLLCGEELYVHSQDDKKI
                     RRLSSIDFPPPETLKYYNGYMHAVNNKLIIIVGIAGMYNAWVLSTEGEILLSRIRIAS
                     WKNILKDDMLFVITGSAGHWDLKSISITTKKDRTIKSFTKLQDVHLLNDGIIINNGDE
                     IVVYNYDNTFYAIPFTY"
BASE COUNT        17374 a         9679 c        11654 g        16984 t
ORIGIN      
        1 atgattttaa cagattattt aaataaaaag aatattatta tcaatccatc tgttaaggat
       61 agatgggaat taattgaggc aatgctcgac atggcaatca agaataaatc gctgaaagca
      121 gagcatcgtg atgcaataaa aaaaacattg tttgaacgcg aaaagtccat gagtaccggg
      181 attggcaagg gggttgcaat tccgcattgt agctgtccct atgtcgatga tgttgttatg
      241 atcatgtcat tgagccaaaa aggcattaat tttgattcta tagataatct accggttcac
      301 attgttattt tacttattgt tcccaaggat aaaatatcac aacatatcaa aacactggct
      361 aatattgcca agctcatgaa tgatgatagt ttacgtgaat cgctcattgg tgctaaggat
      421 gttcaggaca ttataaagat tttaaaagag tatgaaaaaa aaggaagaaa ataataaccc
      481 gggcattatc tcaccattat cactggatga agaagatttt gagaaaaaat tacgccctca
      541 acgtctcagt gagtttatag gacagaagca gttaaccgaa aacttacgtg tatttattga
      601 atcggcaaaa ttacggaaaa agccactgga ccatgttttg cttgcagggc ctccaggact
      661 gggcaaaacc acactggcat tcattgttgc caatgagctg ggcgttaata ttaaagctac
      721 ttcggcaccg gcaatagaaa agtctggcga tatggcttca cttttaacca atctggaagc
      781 aatggatgtg ctttttattg atgaaattca taggctttcg ccagcgattg aagagctttt
      841 gtatagcgct atggaggatg gtgagcttga tattatttta gggcagggtg ttggtgcaaa
      901 gagcataaaa ttgcaacttg ccccctttac cctgattggc gcaacaacgc gcacaggtct
      961 tttaacctca gcgttgcgtg ataggtttgg tataccgctg cgtcttgatt attacgagat
     1021 agatgatctg gaaactattg ctttacgaac cgcagcaata atagggtgta gcatagaaga
     1081 agaagctgca caggagattg caaggcgttc gcgtcggaca ccacgcatag tcaatcgcct
     1141 tgttaagcgg gtgcttgatt ttgcgatagt tcataaaaag gatatgattg ataaacaaat
     1201 tgccctgtat gctctggata aacttcatat tgatatgctg ggattggatg atatggacag
     1261 aaagatattg tccaccatta ttgataaata tgacggaggc ccggtgggga taaagacgat
     1321 agccatatca ataggtgaag agattacaac gctggaagat ttttatgaac catatctggt
     1381 gcagattggg atgctcaaac gtaccccgcg aggaagggtt gcagcacgcc ttgcgtatga
     1441 acatttaggg cttgcctata aagggataga ggatatacaa acactctttg aatagtttat
     1501 agctttataa gcttatctgc catcattaat gtttcagtaa tggtatacat atttgagata
     1561 gttcccactt taagcagttt tgtgcgttta taatactcaa ggcaggtacc acaattgagt
     1621 atctgtaccc cttttttttc aatctgctgg agtggttgaa taaccggtga atcctcggtt
     1681 gttaaaaata tgcccgagtg agcaaagatt atggttgttg ggcacacatt ggcttcgttc
     1741 atggtgttga tgaatgattt catgaggagc tggcctagtt cagtatcgcc cctgccaaat
     1801 tcattgctgg ttataaaaac aacaacattt ttcccattat tcattgatga ttgtagtttt
     1861 gctgaagcaa acggtttggc ttctattgct ttatgcaata caatagtgaa gtaatcatct
     1921 tttatactgg tttgtacacg gtatccacta tgttcggcaa aacgggtaac attgcccgct
     1981 gatgtttggc tgcttactaa cacttccagt tcgtcagggt tttcatcctc tattgcagat
     2041 tttgtcaata gtaccggctg tgggcattcc agtcctcgag catctatcgt tttcatataa
     2101 aggctccttt taaaaaaatt tttaattttg ctattgacca atacatacgg aaatatgata
     2161 taaacaataa ctatcgctcc aatattgtaa atcattgtaa ttgtgataca attattaatt
     2221 aatgttttgt ttacaaacgt ttttttacag gaattgtaat ggaaaagatt tttaaacgag
     2281 attttaaata tagagaaata ccttataact atacctcatt ttcagacaaa gagattatag
     2341 taaaatattt tgatgcccat acatgggatt tagtggaagc actacgggaa aaacgacgta
     2401 caggcagaag tgcaaaattg ctgtttgaaa acattggaga catctttatt atcgatagaa
     2461 acccgtatat tcagtttgat attattgaaa atcccacgaa attaaaaaat ctgcaaaagc
     2521 gacatcagcg aaggcttgcg acagtcaaag aaggtgctaa tggagatggg caggttcttg
     2581 aattgattga taagattgaa aaattggatg atactgcttt taaaaaatta aaagaatgta
     2641 aaaagcagca agaaaaaata ttgaataaat taaagcatat tacatcggaa aaaaatatac
     2701 aatttagtcc ttttcaccga gctgctcatg ttaccgatgc tactgattgg cgggttgaat
     2761 atccctttgt tgttgtttac cctgatacag tagaagaagt acaggaactg gtaaaggctg
     2821 caaaaaagtt agggcttgtc attattgctc gtggaggagg gacggggctt accggcggtg
     2881 ctatccccct tcttcccaat acgatggtca tcaatactga aaaatttaac cgtattggca
     2941 ggattgaaaa gacatacctg aacggtaacg aagttcctgt tataacagtt gatgccggtg
     3001 cagttaccga ggatgtaata caatactgtg aacatcaaaa ttatatcttt gcaactgatc
     3061 caacttctgc atgggcatgt accattggcg ggaacattgc tgaaaatgct ggcggtaaaa
     3121 aatgtgttat gtggggaacc tgtattgata atgtgctgtc gtttgatatg gttgaccaca
     3181 atggagaact tattaccgta aaaagggtta aacacctgta tagaaaaata tttcccaatg
     3241 atgaggttga gtttgaggtt ataaaaaata ataaagtagt caaaaccata acactttccg
     3301 gacttgatat acgaaaaaaa ggattgggca aggatataac caataaagca ctgggcggct
     3361 tgccaggatt acaaaaagag ggatgtgatg gtattattgt ttcggctacc tttgttttgt
     3421 ataagccttt taaatttacc cggacggtat gctttgagtt ttttggcaac aacatgatta
     3481 atgcgtcgaa agcaattgtt gagattgtta aaacctttga aaatgatcct gttgtgtttt
     3541 taacagcgct tgaacatttt gatgagcagt atgttaaggc aattcagtat aaaaataaat
     3601 cgcttcgtac agaaatcccc aaagcggtac ttgtggtaga tattgaaagc gatgaagaag
     3661 aacatttaga aaaggctacc ttagatttag ttgcaaaggt taaacagtac aatactgaag
     3721 gaataattgc aaaagatgca gggatgcgtg aaaaattttg gcaggataga aaaaatttaa
     3781 gcgccattgc cagacatacc aatgcattta aattgaatga agatgtcgtt atcccactgg
     3841 aacggttgca ggacttctca gattttattg aaaagcttaa tattcaaaaa gaattagaga
     3901 acaatattca ggtgatagct gttcttgcag attacctgca ggaacgtatt aaaaccgaag
     3961 atgacaaact atgtctggaa cgatgccagt caggggttgc tcaattaaaa acgatgcagt
     4021 cccggtacat ggatatactg gataatattg atacgcatgc aaaagattat tttaaatatg
     4081 attcagaata tgctctgaga atggattctg tttttcagtt actccagaat aatgaattaa
     4141 gcattaattt tgatgctgat gttattgaac cactgtcaaa acttttctat ggatatgatg
     4201 atattttaaa cacaatttac cagttacaag ctgatacaca aaagcgtcgt atcatagtag
     4261 caactcatat gcatgctggt gatggtaata tccatgtcaa tattcctgtt cattcaaatg
     4321 attatcttat gatgaaagat gctgaagaga ctgcagccaa tgttatgcaa gctactgtta
     4381 catttggcgg agtggtaagc ggtgaacatg gcatagggct tacaaagata cgatttattg
     4441 atgatgatac attggcaaaa tttgctgagt acaacagatt agctgatcct gaagatcttt
     4501 ttaatccctt aaaattaacc agggatttta atctggaaac tgtctataca ccttccttta
     4561 atcttcttga gggtgaagca tttattttaa aagcaaccga tttagaaaca gtttttaact
     4621 ctattgctac ctgcattcga tgtggtaaat gtaaatcggt atgcaacacc cattaccccg
     4681 atggtatggt attttacaat ccaaggaaca aaattttggc gacagctctc gttatggagg
     4741 ctgtaatcta tgatatacag acatccacaa gcttgagttt taagcatttt actaatttac
     4801 gtgagatttc aaattattgt accatctgtc ataactgtca aaaaccatgt ccggtctcta
     4861 ttgactttgg agccattacc ttaaagatac gatcaattct ggaggctcga agaaagtcaa
     4921 cattcaagcc cgtaacatca tttacgctct tctatctgaa acaaaaaggg tactatataa
     4981 ataaactatt cagggtgctg ctgctacgat gggtgtattc tttacagcgg tttggatatt
     5041 atatcgtcaa acctatccct catgtcgtgc agtttatttc gccctatatc gctatgatgc
     5101 tgaaagggag attgcctaag tcagggcata agtctttgcg cgatctgtta cagttaaaaa
     5161 atacaaatac attttatgtg ttcaggaata agaataagcc agttataaaa accgtggtat
     5221 attttcctgg ctgtggttca gaacggatgt tccctgatat tagtattgca actgttgctc
     5281 tgctgtatta tgcaggagta agggttatta ttccgccaac atatctgtgc tgcggctatc
     5341 caatgagagc aaatggtaaa ttgaatcagg cacggataaa aaccaatgag aatagggtcc
     5401 ttttccacag aatggcagac actttttcct atctggatat aggagatatt gtagtatcct
     5461 gcggtacctg ttatgagatg cttctggatt atcaattaga gaatgttttc agaggagcaa
     5521 ggctcattga cgtcaatgag tttatagccc gtgaaggatt atattcaaaa accatcaatg
     5581 agacactgat atatcatgaa ccgtgtcata caccgttaaa agctatggga tatcaaaaaa
     5641 cattttccct gttgctcaat acaaagccac tatcggttcc taattgctgt ggtgaagggg
     5701 ggacattagc attatccaca cctgatattt caaacagttt acgtgaacgg aaagaaacca
     5761 atataagggc aaatgttgtc aggaaaaatg tcatggtgct cacaacctgt ccaagctgtg
     5821 tccagggatt atgtaagatt caggatacaa taaaggttac cggtaaatcg cttgttgtat
     5881 atatagctga acaatgtctt ggtaaagagt ggaagagaca gtttctgaaa gaaataaaag
     5941 cacagtgctt tgacaggtac atttattgaa actggaaacc agcagtgcaa aaaaatgtca
     6001 ttggcaacct tttagctggt aatacaatca tgcttgtggt ctttgctggt attctcaaat
     6061 agatttaatg aaaaaatatc gaggattttt gctggtttca gctttccagc gctcccattt
     6121 ttttttacag gttgtgtgcg atatctggat acgtatcgat aacagtatat gtctcaccgg
     6181 ggtcattctc cagatcatac accactgtcc agcgatatcc cagttgatct ttgaccaacc
     6241 gattaattaa attggttcaa tctaacggta taagccatac atagcgatct atttttgaat
     6301 gctattcaca tttaccagaa cgaatatcta ccatgccctc atgattatcg gtgcaaaaat
     6361 tgtaatattt tcttgacaaa tactggctat tattatatct tatcatcaga ccatcagaat
     6421 atcagaggta atatggatgc attacaacct atcaagcggg tgaagctctc ggaatctatc
     6481 gtggatatga ttattgaaaa gatcaaggat ggagggtttg cgccagggac aaaactgccg
     6541 ccggaaaggg atttaatggt tcagctgggt gttggacgct ctaccctgcg tgaagcaatc
     6601 cagtcattag cgcttatggg tatccttgat gtacgccccg gagaaggtac ctttatacgc
     6661 ttgatacggc gtgaggatat cataagccca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn gattcacata ctatcatatt tcaatcaatt aaagatggtg atgaaaagaa
     6841 agcgtaccag aatatgcaca ggcatatcga ggatgttgtg atatggtata aaaaactggg
     6901 tattatttaa taattttttt ggagggcgcc atgcgataca tgtatagcat tattttttta
     6961 ataatacttt ttgtatcgtt accacttcaa gggtattcag caattgatac atttaataat
     7021 agcaatcacg atgtaactat tactccaaca ccgctggtat ataaaaaggg tacactgctc
     7081 taccgccgtg tacaggttgt atttcccagt aaagaaacta ttcaaaagct gaccatacgg
     7141 acatcagttg atggttctat cagcacaatg catacagata acccggatac cagtatcttt
     7201 ttagacatca aggtgcctga tttaaacacg cacaaagctg tttttaccat gcagtgcgat
     7261 actaccactg tcaaaaaaaa gattacaatc cctgctgtta aaccatggaa gatatactgc
     7321 gttccattta cccatgttga tattgggttt acccaatcgc agaagaatat tcgtatacaa
     7381 aattacaaaa acattgatat tgaactggaa ctggtagaga agacaaaaca ctatggtaag
     7441 gatgcatcat ttaagctttt taccgaggtt agctgggcgg ttcaggaata tttgtcggat
     7501 gctaccattg aacaggataa aaagcaaaag cttatcaaag cattacaaaa gggcgatttt
     7561 gagctgggag cattccttat ttcacaccag aatcgattta tgagccctga cgcaatcata
     7621 gcttcactgc aatcaacctt taaccttgca aaaaagtaca atgtaacaat acatactgcg
     7681 tgtattcatg atgtcatgga tttttcaaag gtaaccaaga tactgtgtgc aaatgacatc
     7741 ccctatcttc tggttggccc aaatgataca cgatatgtac tccctccgct gttttatggt
     7801 gtttcgccgg ataccagtgc gaatgtactg gtatggcaca gtgccggact taatgggtat
     7861 ggcgaaaact ttgaccttgc tatgcgtcta aaactgccct ttgatgagca agcatttacc
     7921 actatggaga acagcattgc cctgcatctg gcacaacttg agaaagggta ccctaccgct
     7981 gaaatacaac agtattttga ttatggcaat gcacagtggg actatcccta cgatgcatac
     8041 cttttgcctt actatcctgc acagggtggc gacaatcaac ctcaaaacat cgtcccctct
     8101 gaaatagccc gggaatggaa taaaaaaatg ataaatccac agattatcat tgctacacct
     8161 tcgcagtttt ttgattatgt gcgttcgcgc tacaacagtg taataccgcg catcaagggt
     8221 gagatggcac cattctgggg cgagcagata tatctggact ttattcaggc ggacccccaa
     8281 agacttttga taagctacat gtttgataga ggaatgtatc agcttgcttc aactgttgtt
     8341 gatgctatgg tgcatgataa gcccattaat gattctttcc atagtattgc tgaatcggca
     8401 tacaaagcaa tactcctcaa taatgatcac aatccccgtc ccgttccttt tggtaaaaca
     8461 cattatacct cggatgatgt taaggactgg atgaataccc gctccgaatg ggtaaagctg
     8521 ccacagatat tgatgcataa ggctggatat agtgaggctt tccatgacgg gcggagctgg
     8581 gaaaaaataa agtataaaaa aaatataatt attatcgata gtcattttta cagggtaact
     8641 atcgaccaga aaaaaggacg cattaccggt gtatatgata aagagctgca aaaggagtgg
     8701 atacgaaaca atgctcagta cggatttaat cagtatgtac agacagtgcg gggcgaaaat
     8761 ggtgcacggc gggattatta tcaatgcatt gaaggattta aaagtcctga tgttttggtt
     8821 taccgttatc atgatgatta tagacttatc attgagggta cggttaaaga gtattacaag
     8881 gggatggaac tgattgcaga ttttctggat aaatcatttc atgtttcatt gccgccatgg
     8941 ctgttgaagg tggtgtattt tttttaccag ctttttacac cttcgcttac ccttacccag
     9001 gagattgtac ttccagcgga tgaaaaaaag attgaattga tacagacatt cagcgggtca
     9061 cagccacaga tagctgaaca ttgttttgca tatcctttaa catcggatgc actgtactat
     9121 gatgctgcat ttactccatt actctgggaa tccatttata atggtggcaa tatcattcct
     9181 gctgcaaaga atactgcacc gtttaaaagt atcaatgaca ctttatatcc cttccagtgg
     9241 atgtatggat tgccgccatc gttccactgt gatgcgttta ccatggtaag ggatgatgca
     9301 atgtatgctg tatttatgac aaaggacagc aaagcgatag ttcctgagtc aagttattcc
     9361 agggatagta atggatttta tcattgctgc attggatgga cattatgggg aaaagtagga
     9421 cttgggcaga gcctgaagga taaaacagta ttcaggagta cctttacaac ttttagcgct
     9481 gatagcatca cctatgcact ggcaaaggcg tatgataaaa gtttccagga atatggttat
     9541 acaacctctt caccggtaac agtgtccaat ccagcaattg ctgtggtgtg gatgtacccc
     9601 ataaccaata aaaagtttac cgttggacta tttgaaactg ccggacaatc acagagtacg
     9661 cacataagta tcaaatccat acagcctata gcagtttatt attctacctg ctatggggaa
     9721 aggaaaggtg atgtggcaga taaacagtta ctatcgctta agccacatga attacgtttt
     9781 gttactattg aaattcaata aaaggaggct ttatatggca ccagcgtata cccatgaaca
     9841 aataattgaa ctgtgtaaaa ggttggagaa aacctatacg cctgcagtat gtgatacgct
     9901 tgatgagatg ggatttatgc atcaggcgat gtgctcagga tttagtccta tcatgccaga
     9961 ggctgctatt gctggtcctg cttttaccat ggaggaagca aaaacccgca aaagtacgcg
    10021 cctcaatgag tatgaccctg agtttgtagc caaggttctg gaagatcttt ttggaagcat
    10081 gcaaaaaggt caggtggcag tggtcaatgt taacgagttt tatgaagcag gtgcatgggg
    10141 tgagcttatg tcaacaacct gcaagtatta tggtggtgtg aaaggagcgg tggtggatgg
    10201 tcccatccgt gatattaaca gggttataga ggtagaattt ccagtatggg cacgtggcaa
    10261 tatacctact gattctgttg ggagggttga ccttgtaggt attggaggcc ccatctggtg
    10321 tggtggtgtc agggttaatc cgggtgatat tatatttgct gattgtgacg gcgtggtggt
    10381 aatccccatt aacgatgttg atttgaaaga ggtggtggaa aaggcagaag aggttgtaac
    10441 agcagaacgg cgttcacgag aagagatacg agggggaaaa tctctggtgg agatatataa
    10501 aaaatacggt aaactataaa ttatctggag ggagccatga gtgcgatatg cgtaacagga
    10561 tgttcgggct atttaggacg acaggttgta aaaaccctgt tagagaaaaa ctttgctgta
    10621 acagggatag acctgaaaga catgaatgaa ccatcagtta tttccaataa aaagtttacc
    10681 tttattaggg gtgatattgt acagaaagat actgtgcaca aggcatgcaa gggtgtacag
    10741 gctattgtcc attgtgcggc ggctttagcg cagtttgtgc gcgatgaaaa ggacatgttt
    10801 gccattaatg ttacgggaac aaaaaatgtt gtagaggtat gcaaagaatt aaaaattccg
    10861 caacttgtct ttatctcatc ggtagaggtg tatggcattg atgtcccggt cccctgcagt
    10921 gaagatgttc cgttacagcc tgtatgccct tacggcaggc acaaggttga gggggagcgt
    10981 atgtgcacgc agctggcaga gaaaggatat ggtgtttcaa tagtgcgccc accaacgatc
    11041 aacggtcccg ggcagaatga gccattttta gttgcccaga tggaagcggt gtacaaagga
    11101 agtccggtta tactgccagg tggtggtaaa acaaaattac agatggtgga tgtacgcgat
    11161 gttgcaaatg caatttattt gtgtcttgaa aaaataaaaa aaggtataat cattgcaaat
    11221 gtttccagtg ataatgtgcc aacaacgcga cagcttgtag aggcgctctt taaacatgcc
    11281 ggcaaaaagc caaaaataat ttcagttcct gcatggattg cccgtactat tgtaaaggga
    11341 ttatctgcca taaaactatc gcccattgaa ccgcaacatc tagaaatagc attacgcgac
    11401 tatgtctttg ataatacacg tgcaaaaaaa ttattaggct ggcagcccga aaaaagtgat
    11461 attgaaagta ccatcgacgc gtatgagtac catattaaca tgcgggggtg ggtatgaaga
    11521 ttgaatttac ccttattggg caggcaacgg tatgtattaa ccttgatggc atcgtggtta
    11581 tgaccgatcc atggtggggt tcctttgaat ttttacgggc agttccctta accgttgatc
    11641 ccgaatcaat aatgcctgtt cattatatgc tggtttcgca caaccatgtt gaccactggt
    11701 gtaagcgtgc tatcgatgtt gcagttaaac gaaatatacc aatcataggt tcaacgagtg
    11761 cagtcaaaag ggctgtaaaa cataacgcaa caaaggtaaa ggctatgaca ccagggcagt
    11821 ctgttgagtg tgaaggattt accgtcattg ctgttccagc ctttcaccct atggcaaagg
    11881 atgccgttgg attcattatc aaaaaggata gcatggtgct gtatttttca ggggataccc
    11941 tgtacacgga agagttaaaa aaggctctga ccccctatac aataactatc gccatggtgc
    12001 aggtggcatg ctctacctat cctatcattg gcaaagatgg gatggacctt cagcaggcag
    12061 cgcagtttgt gagggaatgc aagccaacca ttgtgatccc catccattat caggttaagg
    12121 gtaagcatct tgttgatgaa acgttgcaac aatgggatgt ggaagcgcaa aaagttatat
    12181 tgccccatgg aaaaggaata gcggttacgg tataggcaaa ggcagggaac actatactct
    12241 ctgcgtcttt aaaaaacgtt ataagggggg atactaccat gatagagaat ccaccaaaag
    12301 ggaaacaatt actaaaggtt ttaggtccgg ggcttgtatg gtcggcaagc gccatcgggg
    12361 tgtctgagct tgtttttgcc actcgtgcag gggcactttt tgggtattta ttgctgtggg
    12421 cacctattgt ttctttgatt atgaaatatg ccatatttga aatggctgga cggtatacca
    12481 tcgcaaccgg tgaaaatgtg cttattgcgt ttacccgggt agaattagat tttaaacttt
    12541 ttaaattaaa aacaggatgg atcattgtat tattctggat agtgtttatn nnnnnnnnnn
    12601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aaggaatggt gccgcagggt gaaaagcttg
    12721 ctcttcagat atcacagatt gccggcgaaa actttggacg cataggggag ataatctttt
    12781 tagtggggat ttttggtacg ctgttcagca ccctgcttgc aaacattgat gggctatgcc
    12841 gtgttgcttc aaattccgta tggtatcagc gtcgtaaaaa ggatgataca aagttacact
    12901 attaccgtct gtttgtaatc atatatgtac tgttaacagc tcttttctcg gtattatttc
    12961 cggcgccggt tattctttta caaatctcag cggtgattga tactatattt ttaccgttaa
    13021 tcatagggct tggtatctat atctgccata agcatctgga agctatatac cgcccgggaa
    13081 agctgcttac cataacaggg gtgctgtcgg tgctattttt tgtttttttt attgtactat
    13141 ttttctatgc ggtgataatg aaggtagatt tttcaatgta accatgtaca ctattaccca
    13201 tggatgatag ggcaaaaaat tgttgtgggc gatagttact gatgatggtg ctggatgatg
    13261 ggaaaagagc atactatcac aagtcaattt attgcctggg gtgagatact gctgcgatta
    13321 tcacctccag gctacagcag gctttttcag acaaatatgt ttgaagcgta ctttggagga
    13381 gcagaggcaa actgtgcggt gacactttca caggtgggaa taccatgcag gattgtttct
    13441 gctctgcctg ataccgttct gggtgatgct gcaaagcgtt atttacaggg gtttggcgtc
    13501 attactgaca acattgtgat gaaacaggat acgcgcatgg ggttgtattt ttatgaacag
    13561 ggtgccggac tcagggcttc acggcttatc tatgacagaa acaacagtgc atttgcacaa
    13621 ttacagcatg aagacattaa ttggaacgcc ataatcccaa gcaatgcatg gtttcataca
    13681 acaggtataa ccctggcgct ttcaaatact atctgtgacg aaagcattaa agcagcaagt
    13741 attgtcaatg aaaaaaaggg ggttgtttca tttgatatta actatcgcca gacattatgg
    13801 aaaagtgttg atgatgccag ggatgcattt ttacggctga tgccctctat tnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngctgcaggg tttatctatt cattgataca
    13981 acagcaatcg cttgaaacag cagttgatac tgcccttgcc ttgtgtgccc tggcgcatag
    14041 tgttcatggt gataatttta ttgttggtaa agcagatatg gacgcaatca tacagaataa
    14101 aagcagccga ttgctgcgat agataatgag acaatgtgct attacattgc caatccgcac
    14161 tctatttacc atgatgggat gatatacatg gttatgcact atgatgcttc aggtacttga
    14221 tatatttttt ttacttttcc ataccctgtt tacactcttc aacatgacag ggtggatttt
    14281 tgcaaggaca cggaagatac accttgtgac gatgcttcta accgcttttt catggtgtgt
    14341 actgggtata tggtatggct ggggctattg cttttgtacc gactggcact ggcaggtacg
    14401 gaatgcactg agaaaaccta ttgtatataa ttcatatata cagtttttac tccatgaaat
    14461 aacaggctat actcccgatg cgcacataac tgatatcacc gtagccggtg tttttattat
    14521 ttgtttatgc ttttcagtaa tcctcaatat caaagactac acagccaaaa gaaaagcata
    14581 aacattatgc cttgctgaca gcttccagaa gtcctttcat gtacccaata gcaaaaagcc
    14641 tccctatgga tgaatatgct ggattttcgt tgctatctcc ttccatggtg gggacatggt
    14701 cagggcgcat gacaccgtca aagcctgtat catggtatgc tttcatgcat tggtacatat
    14761 cgagcatgcc atcatcatga aaggtttcgg taaatttata gggagtacct ttcacatttc
    14821 tgaagtgcac aaagaatatc ttaccctgtt tgccaaagtg atgtataact tgaggaaggt
    14881 tgtcggtcat gagagcaaaa tttccctgac acagtgcaat accattgacc gggcttggat
    14941 aaaggtcaag aagcttttga aaattatcaa tagagcgcat aatgcgggca acgcccctaa
    15001 tgggcgaaac agggggatca tcaggatgca tggcaagttt aacatgcgct ttttcagctt
    15061 cagggatgac cgcctttaaa aaatattcta aatttttcca taactgctct tcggtgataa
    15121 tgccatattc cgttagcggt gcatgctcca tatattcgtg attgtatgaa gtagccagcg
    15181 cgccgccgcg tataggggtt gtggtggaag tacgtgtcca gccaaaaaca ggcatccatt
    15241 cgtaacacca caccggaatt cccagtgcac ccatattttt tataagcatg atgacattct
    15301 caatctccct gtccctttcg ggcaatccta attttatagc atccattggt gggcgcgact
    15361 caatgacttc aagcttaaaa ccagcatctt cataggtggt ttttatgtgg actaacgagt
    15421 tatagctcca tggccagaaa tcctggggca ttgtttccca gcccgcataa agttgcatgc
    15481 cacctacaac gtgctcaatg ccacattgtt taaccatacg ccacagtgct gatgggtgag
    15541 ggggtaataa ttcagcaatt tttatcatat acgattctcc ttgcataaat gaattgtaca
    15601 actaatcatc atatggtctg atgataatat attaaaagca tcaatcgatg tcaaggatgt
    15661 tttatatatt tgttcataaa taatgctcat accggtgaaa atgatacatg aaaaaagaaa
    15721 ccgtgccgat agtatgattg gatagtaagt tgccttcatc ttgcttatga aaggagaatg
    15781 aaattatgaa actcattgaa acattagaaa caaaactgga tgaattaata catattcatg
    15841 ggcttgtagc attgaaaggt ggtacagaag tagaagacat gacacataat gaactgttgt
    15901 ttatgaagga gattgctatc aatataccgg ttatagtcaa aataggagga cctgaggcac
    15961 ggaatgatat ccgctactgt caatcaattg gtattgatgg aattttagca ccaatggttg
    16021 aatcagaata ttcacttgaa aattttgttt cggccatgat ggatatttat agtaataata
    16081 ctttaccata cttagcaata aatgtggaaa ctatcactgc atataacaat ctgccagcaa
    16141 tctatagtaa tccatacttt ctttatgtta accaggtcac tgttgggaga agtgatcttt
    16201 cacaatcaat gaagaaggca gttgatgaca atgatgttat caatgcaaca gcacatattg
    16261 ttcaatgttc aaagatagcc gggaaggtaa cctctgttgg cggtcagatt aatccacaca
    16321 atgccgaact ggttcagaag gctattcagc cagatagaat aaatacacgc catttagtct
    16381 ttgattgcgc aaaaacacac aatatagcta catcaattat ggtaggacta gaatttgaat
    16441 tggatctgta tagagctttt tcaaatgtcg aaccattaaa aagtactatc taccagaaaa
    16501 ggatagcggt taccactgac agaatggcga ttgtgtagta taacacaaca acggtgctac
    16561 tataacaaaa attttataaa aagcttgacg aaaaacctta tttttaacga tatccttcta
    16621 ctattgatac tacttacagc tattgcctaa aaaggagtgt tgctatgcat tcaatatttg
    16681 tcactataat ggttatttca tcgattatag tgttattgat tatatcaaag attggtaaaa
    16741 atgaagcacc gggtgatgtt aacaataact gggacggtgt atggaaggaa cgcttaaaga
    16801 tagataatta aaaaggatag atatacataa aaataatggt tatgacatga agaactatcg
    16861 attgcagcag tgattttacc gtgctgtaga tatatcctat tgttatacca atgaggaaga
    16921 agatgactgc aaggtacagt gaatgtgcgg ctatccctac caaactatag aataatgcgc
    16981 cgataactat cgccccatat ttcccaaaat aaaaggtaag gttgtattgt acaaatcccc
    17041 tgaaaaaaag ctcttcacat ggtgcaataa tagctaacat gacaagcatt gactcaagcg
    17101 atgacatctg cccctgataa aagtgtaacg tttgagagtt ttgtgtaaat tgtatggcta
    17161 tattacggat agggatcagg ataattacca tgaccatgag gtaaaatatc tttcgttcaa
    17221 aaggaaggtc aaaggcaaaa atccgctctt ttgtaaaaag tcctttatca caatattgtg
    17281 caatgacaat taacaataaa agcacaatgc tatactgata taaaaaagaa aaatacgcat
    17341 actgtattgc tactttcaat aacgggtaca tcacaagcat tggaataaaa actttccatg
    17401 tggggcgata ctgtgaataa ggattatcaa gctttaccat ggcaaatact tttccattta
    17461 aatttagttg gtcaagcaat taattgatgc attggcatat tttaaaaaat ttttataaaa
    17521 aaacaaaaaa attcccccat tcttttaatt ttttaacagt ccggcgtatt aataaacaaa
    17581 ccttacaaca cggagatgct ttgtatgcgt cggattattg ttattgtgtg tttgcttcac
    17641 acatcattag cctatccgca acttctcatt aatgaaatag cctgtaatgt accagcgggt
    17701 gattgggttg agctatttta ctgctctgaa caaaggctat cgatggatat ttcatcgctg
    17761 tatgtaacca tgtattatgg aaccaatgaa cctctttcac aatctccggt tacaatctat
    17821 agctatgata gggcagaaac accatgggat gacaggtttg ttgttgtgca ttgtacaaag
    17881 cctggaatac cagatgagtg tgatgctact ggagatacca atggcaatgg tgtgcttgat
    17941 gtttattgca ataactatat caatagctta tggaataccg attgtgtggt ggcaatagat
    18001 agtgatgatg agccgtcaaa tggcggaata attgattttg tggcgtacgc caacagagac
    18061 ggtactctta accagtcaat tgaaaaatac ctcaaaagcg ctattgacca taatcaatgg
    18121 gtgggtgctt atgccagtga agaatgcatg gttgatattg gtcaaaaagg aattttagaa
    18181 tatcagagta ttattagaaa aaatggtgta gataccaata gcgctgatga ttttgtcatt
    18241 acctcgtatc aaacgccagg gcgccccaat attttgccaa taaatcattc aaagtccgaa
    18301 tttataaaaa ttaagcgcac gatagtcatc tctaaaaaaa atatgcagcc gcttaatctt
    18361 tttgctatcc agccatgcaa gttcaggata cgcctgtttt cctctgttgg ctacaaaata
    18421 tacgaaagtc cactgattaa cgcattgcct ggtgtccagt tacttgattg gacaaacact
    18481 atcaagcatc ttacctgtgg tttatatctg ggtattgctg aggctcagga caatgccaac
    18541 cgcgagagta aaaaatttta ctgtatcgtg gttgagtaat gggttcattg ctgctgacaa
    18601 taaccatgtt cataggcgca tttgaaaatg catataaagt agcattaccg ttacatgtgc
    18661 aggacccatt attggggaaa ttgtacaaca attatcctgc gtttgtgtgc agccttagtt
    18721 atgcaaatcc ttttggtttt cctcagctta aggaaacaaa tacaaatgtt gcctataagg
    18781 gtaaaaaata tgatggcggc atacggtggc attattttgg cattcctgaa tatcgtgaag
    18841 atacactgtc aacagggctt gattgggcga tagctcactg gataaaggtt ggctgcaggg
    18901 tacatgacta tatcctttca ataaaaactc aagagtttac cacaacaaaa catagtgttg
    18961 attatggatt gctggctacg gtaacaccct ttcaacgatt gtcgtgcacc ctatttacca
    19021 acaatataca gcattacggc gatgatgagt atattacgcc aactaccata ttttcatgcg
    19081 atgctgatat ttttgatgga tgtggtattg aatatacgct tttgtacaat ggggatatac
    19141 agcaatgtat atggataaaa ggatatataa ccaggcatgt aagcgtactg gttggataca
    19201 gtaaggaact ggaattatct acagccggtg taaatgttgc atggggcaat atccttcttt
    19261 actatatgct tcagcatcat tcatttctgg gaaacacgca tagatttggg ataacctatg
    19321 caggtgcggg atatgttttc ccggctcata gtgaatataa acaaaatact gcaataaagc
    19381 tcaatataca gacatgcagc caacaggagc ttattgctct gggtattgca cctgatgtct
    19441 tgtgtcagcg tattatcaaa tatagagaga tgtttggagc tgtcagcaca agatcattat
    19501 atcagctggg atttacaaca caacagatac gtcaattgca ggaggttacc tgtaattatt
    19561 gtgatgatga caaggacgaa aaaaaggatt accataagaa atatcaaggc agaacatata
    19621 tatcaaaaga agagcagaat aaaaaaataa aaaggctctt tcagtctatg gtagctgccg
    19681 atataccgtc ggatgttgcc atagtgctgg ctgaggaata tcaaaaggcg ggtataccgg
    19741 ggatactgcg ttcaccatta tatagaagtc ttgataccta tcagcaaaaa atggtcaggg
    19801 ccgcatgcgg aatacaatag ttgtagtttt aagcatggta tatacggtag tttttattca
    19861 tgcagtaatg gcattgagca ctatatcagt tgaatataga gaggatgcca tggattatga
    19921 agctgatacg caacgatatc tacgttcctc aataagtgtc acaacagatt atgcaaaggt
    19981 ttatgcaaca ttgataaaaa caaataatgg caaaacaacg gcaacctggg cttttagcaa
    20041 tacaggggaa tatgcggcga tagtagctgg atattatttt atccataatg catcagggct
    20101 attgctgggt aagcctgctg tttataatcc cgatccatac tcattccctg tacggagcag
    20161 cgaaacggga atatcactat gtaaaagcgg aaatcctgac tacgcgatgc atgggatagt
    20221 tgcatcgctc tatgatagat taaacaaacc gcaggtacga ttgcgtattg gagcatcatt
    20281 gcaggagaga tttatgcgat ctgatgatgt tgagggtaag ctgtatccct atagtattgg
    20341 tggtttgctt tcgcatgaca gcagggatgg attatattcc gaaccagtta acattataac
    20401 ctattttgct aatgtaacgt tgcagccctt tgaatatgtt gttttgcagg gatgctatta
    20461 ccataccgga ttatatcatg agaatacaaa aatagtattt gataaagata atgagaatgc
    20521 aacagcaata cagtcgtttg gagggtatag cctctatgga agatatcaag ataacatact
    20581 aactgtattt actgagtggg ctatctcaca aaaggatgta aaaggcaata gcacttcaac
    20641 atcacaatac aacaatgctc acctgtatgg ggttaccgtt aaaaagaaag ggatacaatt
    20701 gtgcagcata atacaaaaaa cagaacgata tttttatgct ccattcagca gttcatttgg
    20761 cggtaatgcg ccgcgtgata tttattatta ttcagctcag ataaaaccaa tcaggtggat
    20821 gttgctttca tggacataca tggaccagaa ctatcttgta ccatcaacct attatgctga
    20881 atatcctcat aaacggctcc attgtatacg aactcagttt accataaacg cctttaagat
    20941 acaaacggat tatcgcttta tgacatttta caaagctggt gcagagcagc gggattcgcg
    21001 atatcaaaca ggaatggaat ggatggttgc caaaaaagta tcactgaata tgaaatacgg
    21061 gctatacacg ggagaggaat cagcatggta tatgcgatca ggctatacta tacagtgggg
    21121 ttctttacag caggcgatga gtgttgtcta tgtaaacagt aatgggagga aggtgtactg
    21181 tgcaatgctt cctttagcaa acagcaatat actcaccgag gtttgctcca gtacagccgt
    21241 ttatctggct atgcgggttg gctatcgcag caagttatgt cagctgtcat tgaggttatt
    21301 atcggaacta tcgcacataa aaaatgctaa aggtgaattt ggtgcaactg catggtttta
    21361 gataaattga ttctatagag tagttgcact aaaaggtatt tgtggtataa tggttaccgt
    21421 atggatgaaa aaaagattga acaagtacat caaatactga aaaaactcca gccgtatatc
    21481 gtggcatatt caggaggtaa tgattcaacg ttacttgttg caatatccat gcgctggaat
    21541 cttgattttt gtgctgtgca ttgcatcagc gaatttacca ttccgggtga aacagaatat
    21601 gcccgttatt ttgcacagca ccatcgtatc ccctatcacg aaattacagt atcggtaatg
    21661 aacaatagcc agattatagc aaatactgaa atgcggtgct accactgtaa acatgaaatt
    21721 tttaagaata tggttgcgta tgcacacgct aatggtttta taaatgtcat tgatgcaagc
    21781 aatgcttctg atagtaacga ttataggccc ggaaaaaagg cgctggcaga attagggata
    21841 ctatcgccgt ttgttgaggc tggcataaca aaagatgata tttatagtgc tctggcagat
    21901 atacagatac cgcacaaaca attttccaac tcatgtttag ccacgcgcat accgtaccat
    21961 accagcataa caaatattat gcttgatagt attgcaaaag cagaaacttt tattgccatg
    22021 ttgggattta cagcggtacg ggtgcggtat cattatccca ttgcacgtat tgagatacca
    22081 gaagaagaga ttgcatattt tatggatgca accctccgta ataaagttgt tgcatttata
    22141 aaggagcttg gttttttata cgtaacggtt gattgtgaag gtttttcatc aggaaattta
    22201 aacaggatgt taccacatga gtgatatatt agcagctttt gttgcccgta atactaatga
    22261 aatacaaaca aatctggaca atatacagga atgtccattc cccaacatga tgccgcttat
    22321 catcaatgat ggtgtggaga ttgaatctgc atctgaactt gctgataccg gaatacattt
    22381 tgtcattcat gaagaaccgt taggatatgg aggagcactg gtcagtgcac tatcgtatgc
    22441 acgggatttt tcattacact acctatttat aatgcctgtt gaagcgttta ccggctggca
    22501 ggcaatagca acgtatgtcc agcattttga aacatcagat attcttacag ggaacagact
    22561 accctcctca tgcagtgccc ctgaagggtt ttatggggaa attgttacct gcctgaataa
    22621 tggaacagga ttgcatatcg ttgatcccct gtcacctgtt aaagggattg ccatgaagca
    22681 ttgcgatagt tttgaaataa cagaatttcc tgatactgct tttttacaat tattaatcca
    22741 ggcacgtcac ttccatatat caataaagga gtttcaatgc tcgtatccag aatcgcttat
    22801 tacatcgcag atgcaggatg ttgaagactt gcccaatata aaagatttta tagctggtga
    22861 gctttatctg tatccgtata tccagaaagg gcattaatga cagtgcaaag aattggattg
    22921 attatagtac tattgttgaa tgttggatta tgctgctcag ctcccgagca tcgcctgcca
    22981 gagcaatatt ctggattacc ttctgaaagc agtgttcttt ttgttggtga tatgatgttt
    23041 gaatgggcag ttaaagatac catgagcaaa aagggtgtgg attatcccct gcaaaacata
    23101 caaagctttt tacattcatt tgactttgtc atggcaaatc tggaaacacc tttgaccaat
    23161 aatagttttg gcgatagttc ctttgataag aagtatatct ttaaaacaac gatggatatt
    23221 gcccccctat tacataaagc aggcatacac gctgtaacca tagcaaataa tcatatattg
    23281 gattatggcg ttggtggatt aaccgacaca cttgttgctc tttcttcctg taacatattg
    23341 tatgcaggag caggtattaa tgaaacaaca gcttctcagc caatattttg taaattgggt
    23401 accatgcaga tggttattat atgctatacg caaatctgtt caaaagacat gattgccagg
    23461 gataaccccg gtgtaaattt ttttgaatta tcaaaggcta ttcaggatat tcaacggtat
    23521 cgttttgcag atttcattgt tatcaatctg cactggggta atgaatattt ttactatcct
    23581 tcttcaaaac agattggaat agcccatgcg cttatagatg ctggagcaga tgttattgtt
    23641 ggtcatcatc cgcatgttta tcagggtatt gaaatatata aaaacaagcc cattatttat
    23701 tcattgggga attttttatt tggatcgatg catgaaggaa tcaatgatac cattgcatgc
    23761 gctttatata ttgcacctgg gggaaaaatt accaggatac gtgtctatgc aattaaaggt
    23821 aaatttgatg atcagattat acaacctgag gtgctgggtg cagatgatgc aacacacctc
    23881 ttgcaccatt tttttcaaat atcaaagccg ctggatggca attttgtaaa cagagcttat
    23941 attactaaca attatatcga atataatttt cgatgattat tccatcagtg ctttcagcac
    24001 aaagtcaaca ctttctacca ttgaattgat ttcataacca ccttcaagca ctacaatcac
    24061 tcttttattg gaatatttgt gtgcaatatc taacagcatg cgtgtgaaca tgtagtatgc
    24121 actacttgat aaatttaatg aggataatgg atcgctttta tgggcatcaa aaccggctga
    24181 tattaatata aactctggtt gaaaatggtc aagtgcaggg attatgattt cagtgaatgc
    24241 acttttatag gttttatcat tgcttcccgg attcattggt atattaagag tatatccata
    24301 tccaggtcca atgccaattt catcaacact accggtcccg ggataaaaag ggaattgatg
    24361 gaggctgatg taaaaaacag aaggatcggc ttcaaaaata tgctgtgtac cgttgccgtg
    24421 atgtgcatcc caatcaataa tagcaatttt tcggatattg tatgtatatt gtaaataacg
    24481 agcagctatt gctacattgt taaatatgca aaaacccata gcctgagcta tctcagcatg
    24541 atgaccggga gggcgtatgg cacaaaatcc attgtgagct tcacctgaca taatatagtc
    24601 gcatacttct aaaatgccgc ccactgccca caatgataca tcatacgttt cgtcgcatat
    24661 tactgtatcg tatccatcaa tacaggttat tccattgggt tttgcattgg ctattgattg
    24721 aatataatta atatcatgag catacgttaa cacttttggg tctgcttttc gtggctggac
    24781 atacagcaat gatgaataat tgttattatt acggatatat ttatctaatt gttcaaggcg
    24841 tttaggggat tcagggtgag aatcccctgt tgtatggttt aaataatgtg gatgaaagca
    24901 atatgctgta tagctcataa ttattaaatg tttgatagtc gaattataat gtacacataa
    24961 cttaataata ttgtaatata atattaaaaa aaatcaagta ttttttaaaa attttagtca
    25021 aaagctttat caacagacca aacttcccac accttaccta ccagatcggg agctggtttt
    25081 aacgtgtctt tgcccggacg ccatcctgaa ggtgttgctt gagttccttt agattcacgg
    25141 accaattgga aagcatggat ttgtcggata gtttccagaa cattacgccc aaccggtggt
    25201 gttaaaactt catannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttgtc
    25321 ggatagtttc cagaacatta cgcccaaccg gtggtgttaa aacttcatac taacaataat
    25381 attatgatgt tgaaatctat gaatatacaa tctgagctac tatcttccta tccctttttc
    25441 gggtatcgca ttctatgagt actatctgct gccatgtccc cagtgtaagc ttaccctgaa
    25501 ctattgggac agtgattgaa gcacctatca ttgcagctct gaggtgcgaa gaaccattat
    25561 catcatgcca tgtatcgtga tggttataat gagcgttgcg tggaatgatt gtctcaagaa
    25621 aaatatttat atctttctgt aagcctggtt cgtattcaat ggtggtgata gcagcagtgg
    25681 aacctggcac aaaaaggcat aatagcccat tgttaatagt attattagta actatcgctt
    25741 gcgcatccat tgttatatca atgatatcac cctgaccttt tgttgctatg gtaatgtatt
    25801 cagtaatggt tgccataaac tgctcacctt tttccccatg gtttaaggct tgatataaag
    25861 caggcaacta ttaatgtagc ggcctgaaaa gtaccaaaaa tcatcaacaa gataaccgtg
    25921 ctgttgaatt gtgggttgtc ataactggca agcccctgtg tttgtgctat atgaacaagt
    25981 ttgcctaacc atggaccaag aaaataagta ccaaaaataa taccatataa acatattatc
    26041 catttaacaa taatccatct gtatttaaac cacccccatg gtgttaatgt tgagtacata
    26101 agtcctgtta caaaacaacc aagtgcgcca ggaatgataa taaaaagatc aacataatcc
    26161 attgtagata caataccata tagttcacca tcagtttttg cctgtataaa aaattgctta
    26221 cctgatactg tgattgcacc gccaacccat aatgaaacac atagtatatg gaaagctttt
    26281 aaccattttt gacttttagg agatagtttt ttcataattt taccctgcat taaaagtttt
    26341 gtaaattgaa atagttatag tattagattt aattacgaat ctttacatag aaatcaagta
    26401 caatattttt tgcaataaaa cataagaggc tgtaatcaat ggttacatac gtttcatgac
    26461 catggttcaa agattacaaa acatataagg caatagcgag gtgcgatagt tacttgaaat
    26521 atgcaaaaat ggatagatga agtttgaaag gagtgccata tctgttgacc ttgtaattat
    26581 gtatcacaac ataaaacatg aaaaaagttt gacaaagtat aaatttatta tagatattca
    26641 atatggttta taataaaacg aagtcatgaa tattgaataa aagtattata ataattatac
    26701 tatatgtata attcacagat gaacatatct gcttgaataa tttttttgca ataaattctt
    26761 ctcatttcat acaataacta ttacctttga ttacatgata atgtgaggat atagctatgc
    26821 ttgaaaggtt gctcaatata aggctattta acaatacagt gaaagagtat tgtgtaagtt
    26881 tgttaatttt tattgttggg attatagcta tttatatttt caaaacaata attataagat
    26941 tattaaaatc atgggcacgg aaaacaacaa cagaattgga tgatattgct gttgaagcat
    27001 ttgaaaaaaa atttatcccc ttgatatata tatgttcatt ctatgtcagt attatacaat
    27061 taacattaca tccttcttta aagaagacat tggatgttat tgtattaata ctgatgacta
    27121 tatttggtgt gatgtttgtt ctcaatggga ttaaaatttt tatttccgct ataatacaaa
    27181 aagaaggcaa tgtaacaaaa caaaaagcat taagagcatt gaatccgttg atacagattc
    27241 ttgtatggtc gtttgcaatc tttattttat tagaaaattt agggattaaa cttaccccgc
    27301 tgattgccgg gcttggcata ggcgggttgg ctgttgcatt ggctgctcaa aatatactgg
    27361 gagatctatt tgcatatttt gttatgctct ttgatcgtcc ttttgaaatt gaagatttta
    27421 tcattattga taaagatttc atgggtacag tagaaaaaat aggtatcaag tcgtcaaaga
    27481 tacggggtat aggcggtgaa caaataatta tttctaatac caaccttgcc aatgctcgtg
    27541 ttcgcaatta taaaagaatg aatagaagaa gggttgtttt taccattggt gttgtgtatg
    27601 agacagggct tgaagattta acgttaatcc cagagattat aaaagagatt atccagaata
    27661 ttgatggtac aacctttgat agagcacatt ttttttcgta tggtgatttt agtttaaatt
    27721 ttgagattgt atactatgtg aacagcaatg attacaatgt atatatggac atacaacagg
    27781 aaatcaatct taagataaag gatgaatttg aaaaacataa cattgaattt gcctatccaa
    27841 caagtcttat ttatgtaaca aaaagcaaga tgtagccgta tggattattg tataattaat
    27901 aaatagataa gtaggtgagt atgcgtttag taattatttc aaacaggttg cctgttacag
    27961 tttctgaaaa aaatggcagg ttgattatta aagaaagtgt gggagggctt tcaaccggat
    28021 taaaagcata tatcgatttt ataaagaatt catcctccga aaattgaata tataatatat
    28081 atggatagga tgggacatta gtagggtttg aaaatgtcct gattattgat ggtgaaaacg
    28141 gattccttgt tgatccctat gatattgatg gatgtgccaa aaaagtaatt gagattctta
    28201 aaaatgaaga gcttagaatc agtatgggga aaaatggaaa agaacatgta aggaagaact
    28261 tcctcattac aagagaactt atggatcatc tggacatgtt aattgatgtg ttattgtaga
    28321 tgtatgtgtt atgataatag taaagacccc cttaaaaaat aacagtagac atgtaaaact
    28381 ttaatgttca ttcctgcaaa atctcgtaaa aaagacttca atcattactt atcataatgg
    28441 taagcctggt aaaacgtcaa tagcaatacc attaaaataa aataataaaa gtaaaattac
    28501 ttgactatta aaatatagct ataacatatc gttaaagatg taatattgta tgataattat
    28561 atgaggttga catgaaaaca tttttttacc ccgagagcat ggttgtagtt ggtgtttcat
    28621 taacaaaaat aaatttagga aagataatac ttctaaacaa tatacggcag ggatataaag
    28681 gaatactgta tggggtaagc acccatgaag gagaaataga gggggtaata gtatataaag
    28741 acattttaga tttgccaaca accccggatg ttgcaattat tataaccccc gctaaaactg
    28801 ttcctgaaca gttagaaaaa tgtggtaaaa aggggatttg ccatgttgtt attgaatcag
    28861 gagggtttag tgaatattct gaacaagagc aaagtatgga ggaacaaata cttggaattg
    28921 caaaaaaata tggaatccag attataggtc ccaattgtat aggaataata aattttgaag
    28981 aaaaggtaat tatgccattt gcatttatcc agaacatccc ggaggcagga caggtatcac
    29041 tgatttcaca aagtgggggc gttgggaaca cttatttaca cgctctcccc gaaaatcata
    29101 tatttatgaa taaatttatt agtattggta ataagttaat gcttgatgaa gttgattttt
    29161 taaactattt agtaaatgag gatgaagcga ccagaataat attatgctat cttgaaggat
    29221 ttaatagagg gaaggaattt tttgaaattg cccgtttatc cgaaaaaccc attattgtac
    29281 ataaatccaa caggtcgcct atcagtgcta agattgctca atcgcacaca acagcactat
    29341 ctgccagtga tgcaatcgtt gatgcagcct ttgcacagag cggagtgata cgagcagaag
    29401 atgaagaaga gatgattaaa gctgtcaaag cgatgcagct tccactaatg aaaggacgaa
    29461 atgttgccgt gttgtcccga tcaggaggac atgccgttat ctcggcagat gcgtgtgcaa
    29521 aatataattt caacctggtt tcattcccgc aatcatatat tgatataata cgcaccattt
    29581 ataacacacg tatcatagcg catcagaatc cgctggatct tggagaaatt tttgattata
    29641 ccatatttgg cagaatagtt gaagaaacta ttaagctgga taatgttgat ggtatactgt
    29701 ttaaccacct ctatcaatcc gaatatgaag ctactatgtc acggacattt ttagataccg
    29761 tagaacggct tatcaaacaa tataataaac cagtcatgat tgccatgata tctgatgcac
    29821 aggaaatgct ggatataaac atgaatcacc ccatgccaat attcaacaca ccatacagtg
    29881 ctgcacatgc acttgcaata tcagcagagt attatcaaaa aaaacaaatg cgaaataata
    29941 gaggtgaaat tgactataga aaaatggaga aggttaaaca agattttgcc gtgctcaatg
    30001 atgtattgtg caacaataaa aatccgttgt tgcatgaatc aattagtttt tgtgaatcaa
    30061 ttggattacc cattgtcaat ggtgcactta ttacaacagt tgatgagatt tatgaaaaag
    30121 aattccaatt tccggttgtg gcaaaaattg tatcacctga tgcaacgcat aaaacggagg
    30181 ttgatggagt caggctcaat ataatggatg ttgaacagtt ggaagatgct tttatagata
    30241 tgaaacagtc actgtatgat tataacccca atgcagtatt taaaggcata tatgtgcata
    30301 caatggcaaa agcaggcgtt gagtttattg taggagcaca aaaggatgca gtctttggtc
    30361 ccattgtaat ggttggatta ggggggatat atgttgaact ttttaaagat atagctatac
    30421 ggttagcccc ggttacaaaa gttgaagcat gggatatgat acaacagttg aaatctgcac
    30481 aattgtttga aggcttcaga ggtaaagaac ctcttgatgc agaagcatta agtgaagtga
    30541 ttatgacctt gagctatgct atttcagcta ttgatgaaat tgctgatatt gaatgcaacc
    30601 ctgttatggt gtatcccaaa ggtgaaggtt gtattgctgt tgatgcgcgt atggttttac
    30661 aaaaacaaac aattgtttca aaagctacca ccagttaaaa tggaaatcac agttttaaat
    30721 gtggtgggtg ctatgagaaa aaataattgc aatttagtat atatatcata ataatgtcga
    30781 tatcgatata ataagcagtg tgaggtggta tcatgcaacg tactgttgaa acacttccat
    30841 tatggaaaaa gattatctat gcattaggcc agctgggatg gtcacttgct tcatttagtg
    30901 ttgggaacct tctggtatat ttttacctgc caccagagga aagtaataat gcaattttcc
    30961 agccctacat ttatcagggg tatgttatag gtgttttgac tattattgga ttgatctatg
    31021 cgtttggcag gctgtgggat gctattactg atcctattat tgcaacaatg tcggacaggt
    31081 caaaatcaag gtttggaagg cgcagaaaat ttttggcaat tggtggactc ccttttgctc
    31141 ttttgagtat attggcattt acccctccgg ttaatggtat gtcaccatgg aacgcggtat
    31201 ggctttttgt tgtggtgaca cttttttact tgttcatgac aatgtatgta acaccatttt
    31261 ttgcatggtt atctgagttg ggccataatc cccgtgaacg gcttttttta agtacgctca
    31321 tttcaattac atgggctata ggatttgcaa taggttcaca ggctgtggcg ctgcaatcaa
    31381 tgtttgaaaa aagctatgga tatagttctc accgagcctt ccagacagtg atggttatat
    31441 ttggtggaat atcactaatc ctcatgtatc tgccagtatt atttattgat gaacgcaggt
    31501 attgtgaatc aaatgtttca actgagggaa cgtttgaagc attacgttca gcttttaaaa
    31561 ataaaaactt tttgttcttc accatttctg atttagcata ttgggtagcg ttaacattta
    31621 ttactactgg acttgtatac tacgtaacag tgcttttaca aatggataaa gcagtatatt
    31681 cacagtatat gattattctt tttgctttaa gttttatatt ttacgtaccg gtcaatgtaa
    31741 ttgcccaaaa gattggtaaa aaaaagctta tgataattgc atttattctt ttttccatgg
    31801 gatatgttat tgcctcaatg ctgggatggt ttccctttga taaaaatatc cagggattga
    31861 tagttattat cattacatca ttaccgctgg caatctttgg gatattgccc aatgcaatta
    31921 ttgctgatat tgctgaagca gatggaatag aaaaaggtaa ttttaaagcc gggatatttt
    31981 ttggagccag aacgtttatg tcaaaaatgg gacagatgat agcagctctg gtgttccctt
    32041 cattaatact gttgggtaag acaccagaga atgatatagg agtacggtta acctgtattg
    32101 cagcatgttt cttttgtatt gccgggttgt tattgtttat gaaatatgat gaaaagaaaa
    32161 taataagcat attggcaaaa aaagagaagg tttaagataa cattcatcac cagggatgac
    32221 cagtctcatc cggttttagc atcagtcaat ccatgaagga tggaaatgtg gatgttgaca
    32281 gttatcctga acggctgatt cagtatctaa ttattttgat tttgggccaa gctcagaatc
    32341 tcgttgtttt atgaataaat aggttcttcg ggactgcgtc cctcaaaatg acaggcatgc
    32401 agggtgtcgt cctgaattcc gattcaggat ctatcatgac agggggtgta taattctatt
    32461 ttcgcgggag atagtattgt tttgtggttt gaaacagcac aattaagtat ttgacaaatt
    32521 tttcatagga tgcagaatac atggtgcagt ttttggtaaa agagatgtat catgataaaa
    32581 gtaaccattt ccagaacatt ttcattcccc attgatgagg tttattcagc gatagatcat
    32641 atcaataaat tggcacggtt aaaacccgct ttcaggaact atcgcatagc tcgtgacgaa
    32701 gaaggattac agcttattga tgttgatatt cgttttattg taaataaatt tccaatgagg
    32761 cttacctata ctgcgatacc ttttaaatac tgtgagctaa aaaagttaaa aggggctatt
    32821 aaggaatata gttataccta tacatttgaa caatctgaca ataaaaccag tgtaacagca
    32881 gattgtgcta tagtactccc tttacggtat tttattgttc agccatttct aagtgctata
    32941 ataaaacaca gacttgctaa agaactcaca attttagaaa aaatagtaaa ccaaaaattt
    33001 aacggatatg ataaaaccac ttatcgttca gagtgataat actctgcttc tggaagtaga
    33061 caatccacta tatgaagagg tgcgggatgc tattcttcct tttgctgaac ttgaaaaaag
    33121 tcctgaacat atacatacat acaggatcac accattatcc ctgtggaatg cagcatcgtc
    33181 gggattaact acaaaagaaa tacttgagcg cattgcgcag tattcacggt atgatgtgcc
    33241 atcaattgtt gtcaccacca ttaaagaaca gatgcagcgc tatggacttt tgacattaat
    33301 aaaacacaat gaaaattgta tattgcaaag taacgatgct catatcattc gtgagatagt
    33361 tacccataaa tccatacaaa agtttattga tagtcaactg tccgactatg aactggtaat
    33421 aaaaccatac agtcgtggac atataaaaca ggctcttata aaaataggat ttcctgttga
    33481 ggataaagcc ggctatattg atggcgaccc ctgtccattt gcattgcgaa cggttacgca
    33541 aagtggtagt ccgtttgtta tccgtgatta tcaacaaaac gcaataaaca gcttttatag
    33601 gcatggcagt gatgaagggg ggagtggtgt tgttgtgctt ccctgtggtg cggggaaaac
    33661 cattgttggg atgggcatca tgcatatcta tcaaacaaaa acacttatcc tggtaaccaa
    33721 caccgttgca ttgcgtcagt ggattgatga actgctggat aagacaacgc tgtcaactga
    33781 catgattggt gagtattccg gtgatgttaa ggatataaaa ccggtaacta ttgccaccta
    33841 taacatatta acctaccgtc gaaaaaaaga agatgaattt aaacatttta aactgtttac
    33901 cgaaaacaac tgggggctta ttatatacga tgaggtgcat ctactgccag ctccagtgtt
    33961 cagaatgact gccgagattc aatcaaaacg caggttaggg cttactgcaa cactggtgcg
    34021 tgaggatgga cttgaaacag atgtatttag cctcattgga ccaaaaaaat ttgatatgcc
    34081 atggaagatg ctggaagaat gcaactggat tgcaaaggca atctgttctg agatacgcat
    34141 tgaactgccg gaaagcatac gctatcaata caccgtggca ggcgacaggc aaaagtttag
    34201 aatagcatca gaaaacgaaa aaaaactata ctacattgaa aggctcctca atgcacatcc
    34261 caatgacagg gtattgatta ttggacaata tattgatcag ttgcaaataa tcaaggagcg
    34321 ttttaactat cctatcataa ccggaagtac accggtaaag gagcgcgaag agctctacaa
    34381 acaatttaaa ggtggtacta ttacacggct cattgtatca aaggttgcaa atttttctat
    34441 tgacctgccg gatgcaaacg ttgccattca ggtgtcaggc acatttggtt caaggcagga
    34501 agaggcacaa cggctgggga ggattttgcg cccaaagcca ggtgataata ccgcttactt
    34561 ttattcaatt attaccgctc atacggttga ggaacatttt gctcataatc gccagctttt
    34621 tttaaccgag cagggataca cctatatcat catgaatgag agcatgttcc atgaaaagtt
    34681 tagcgcttag cctcattctt gttttttctt caacggtatc ctcatggaag ccattgctgg
    34741 tacaaaacaa tggcttttta tattatcatg aagcaaagtg taaaggatta ccagcaaaca
    34801 ttgtcatcaa tgatatacag gttcacggga acatcatata ccttacaaca tcgcagcatg
    34861 gtatttttaa aaataatggt ggcgatagtt actgggaaaa tataacccct gcaagcgcat
    34921 atacacgcac catacattca ccgtgtaatc agtatcgaag catatcagca ttttgtgttg
    34981 atggctgtga ccccgcccgg ctgtactgcg caacaaaata tacgctgtat acatcgggtg
    35041 atgcgggcaa acgctgggag gttataccaa tgacagggct gcctgctaat gcatgcatta
    35101 ctgcgctcta tgcaaagggc ggtcagcttg ttgtggggac atcgtggaat ggcatatacc
    35161 gttatacggc aggcagattt gttgcagtaa acggcggttt tcccaggaag atgtattcgg
    35221 acagcattgg cttttatgaa acaattgcgc atgtttccgg tggctcgccg gtacgggcgg
    35281 caacacgctt tacaccgcag gtgtattcat acggtgcgtc aggctggaag atggtgtttg
    35341 cggggaatgg cggtccatac tatggatgtt ctctttacga agatggctgg gcgatagttg
    35401 atggtgatag gctggttgta aataacaacg gaacatttgt ttatcaggaa ctatcgccaa
    35461 aaaacttttg ttgtgccgta ttgaccgatg gcacccatta tatttttgta aaagcagtaa
    35521 aaaccagcaa cgattccatt cgaggattat atgccgctac gccaacctca tggcaaacta
    35581 tcaaaaagtg tgtggggtat gcaaaacgct caggctgtaa cgccctggtg tttgacatta
    35641 aagatgatta tggcaacgtg tatggcataa acaatgaaac agcagccatg ataggtgctt
    35701 tgcaaacatc actcccatta catgagttgt gcaattattg tcacgccaat ggcatacagg
    35761 ctatagcgcg tatggtggtg tttaaagaca agcgtttatt tagcggtttt aagcataggt
    35821 atgcaattgt tgatgctgca acaaaaaagg catggcaggg aaatccaaag gaatactgga
    35881 ttgatccgta cagcgatttt gtacacaggt atactattgc gattgcacaa caggcacagg
    35941 atgcaggctt tgatgagata cagtttgact acgtacgctt ccctacggat ggtccggtca
    36001 atagatgtgt gtttaccttt aaaccggata atggcatata taagtatgaa gccattaccg
    36061 attttttgcg tacagcaaaa gcatcacttg ctgtgcctgt atcggtagat gtgtatggat
    36121 ttacggtatg gtatgagttt ggacaatggt tggggcagga tattgaggca atgacaggca
    36181 ttatagatgt gctctgtcct atggtatatc cgtcgcacta tgggaggaaa ttttttgatg
    36241 gaaagataac cccagagggt tattatacaa tagtgcgtga aagtcttagc agggcatata
    36301 tgtattcaaa ggcaacagtg cgcccttacc tgcaggcgtt taacatgctt tctccgggct
    36361 ggggatatga ctatatacag aagcagatac aggcatgcac tgataccggt acgcagggat
    36421 atattttctg gaatgccggc agggactatg aggtgcttat taataaaaac aaataaaata
    36481 aacgatgtac atcagtattg atgatcatat ttttggggaa tattgttgaa tctaataata
    36541 actataataa cgtataattg actaaaaaaa ttgcagtgct ggttgatgat attgtatata
    36601 atttgatgaa gaaaggtgca catggtgata gacaaacttt atcccattgt atggaatagg
    36661 caacagtatt gtatggtacc catggaataa tagtttctga taacaaaatg gatgaagttg
    36721 tacatggtcc tgcccatgtc agagagttga aggggttaaa ggaaattatg caaagaaact
    36781 ataacatggt tccttaacgg taccgttgat gaaacagaaa ccattataaa ataattggct
    36841 tcatttttga tactgttatg ccgatagtta ctctatgaga atctctgaag gattatcact
    36901 attttcacta ccatcggcgg tttcgtcaca gccaaagtca acgatgatgg tttggcctgt
    36961 atatgatgat acgcgcgtta ccccaatcaa aggcattgca cggcggttag aaaataatgt
    37021 acagtatgta aaaccaaccc cggatgaaca tgctcgctta ctttcacaga tacatgacaa
    37081 taaagagccg ctgtatacaa acaatgcatt tatacagaaa aacatgcagt atacaattcc
    37141 cggtactttt tttgatgcaa ttgcataaat aatcaataca ccaataaaat atatagtatt
    37201 aaaatatagt tgaataatat gatgttattt tatagtctac cattataaat agaaaaataa
    37261 tagtatatag tatataggag attgtaccat ggattttgaa accactactt gtatttcgta
    37321 tgaacattta aacacacttt atagtttagc agataaattt aatatgcctc ttcactcatg
    37381 tattgtatac cttcttatct atgccgcaaa aaaagaaaaa gcaccagcaa aagcgtttaa
    37441 aggtatttca tatagaagca ggaacaagct taatccgtgg aagagggtgc atttatattt
    37501 agcatttaag gagtatgagt atttactgga tgttaagaaa atatggaaaa tgagtgttgc
    37561 aagggcaatt gcgttttgtg ttgaaaatgt gctggatgag tttgtacaat ttttaaataa
    37621 cttgattgaa gcagaaaggg aaggggatat cgataactat ctggtttacg agataaatag
    37681 aagctaccta tttgaatatg atactaaaga aggtgttcat tgctgccgat tttattgggg
    37741 attgccacga aaatatgccc gatgtaagcc atagattaaa aaaccaattt tttaatcaac
    37801 aatgaccatt catcaaaagc atgtcaaata ggctatttac taaaataaca ttaataccat
    37861 gagcataaat aagtaaggcc aatgtatcgg taaaaaaagg tgtatgaata aaaataaggc
    37921 ttgattttca tatatactaa ttgctataat gcgaaatctc tattattaca ttttgtttta
    37981 tgatacataa aacaaagatg agtaaagatt atattatttc aggaggtttt caatggttaa
    38041 aaaattgtcg ttgtgtatca tggctttatg catggcatgt atgactactg gattactggc
    38101 acaggaacta actgatgatg ttgctttttt gcgtgtaggc tatcagtgga atacagtgaa
    38161 ttttgataag aatttaccgg tcataggtga taatttggaa aataatggta tagcaataga
    38221 aggtgagtat aacctgaact taggtggatg gttgctggga gtttcactgg aatgggcacg
    38281 tttggtaaat gatgatgata gcgactttgt tttttattac ctttcgccaa tgatttcatt
    38341 aaaatttata gctgccggcg gcttatacat tggtcctgga atttctgccc ggtacatgat
    38401 gagtgcagat acaccttcag gtgttgatga acctgacaaa gaggttgacc tgtgggtcaa
    38461 cgctgttgca ggctggatga cccccatcgc cgaggctgtt tatctggatg ttcaggcacg
    38521 atttggctgg aaccttacca aaaaccagtt tgaagatgtt ggtgatattg ataacaacta
    38581 tgattatgct gtgtatgttg gtataggtac acgcacccgt tcaacgggga tatagtatag
    38641 acatgcattt ttatagctaa cagcttagaa aacagtacag gcagggaaac cctgcctgta
    38701 ctgttttcct agtccttttt ttcttccatg tcctctataa aattttttgt aatcatgtcg
    38761 caggggtttt ttaatgcaat ctctttaaag atgtgaagcg cttcagtata ctgttttttg
    38821 ttatagaggg ttaaagcctt cttgaattgc tcctttgtag atgtaaacag cgccactgca
    38881 tcatccggat acatggataa tacctcataa acttttgttg gttgggattt tccctttact
    38941 attaccttac tgatgtagcg gaagttatat tctggaatgt cttttacctg ttcgtagacg
    39001 tcacctgaaa caagtatggt ggtgcgaaaa tgttttgtta agctctctat acggcttgat
    39061 aggtttactg tgtcggatat aacggtactt tcaatacgca tggtttcgcc aattattcct
    39121 aagatagcgc tgccggtgtg aatacctatt ccaatactga tgggtgaaag ctgatatttt
    39181 ttacgctcgt cattatattc agcaatacgt tttaccatgg ctatcgccgc attcaatgcg
    39241 tcttgaggag attgcgggaa aagcgccatg atgccatccc ctatgtattt atcaataaaa
    39301 ccgttatggc tgcgtataat tggcccgata cggttaaggt acgaatttaa aaatttgaat
    39361 gtttcttccg gagacatttt ttcggataac gtggtaaacg aacgaatatc tgcaaagagg
    39421 atggtaatgt ctttgagcgt ctggtcgcca agctgcacat caaggatgct ctgtttttgc
    39481 aaaaatgata aaaattcatg cggaacaaag cgttcaagcg atatgttgag atcgctaaga
    39541 tgtgcggaaa gatgctcaac ctgcaaaaaa gaaaatgaaa agatgcgtgc aatagcaata
    39601 gcctgggtaa atataaaaac aaacaatcct gcaggaagaa tatacatggt tggcaatata
    39661 ttttttgcat agaggatgtc attaacggct gttaccataa atagcacaaa ccctgcaagg
    39721 aaatagtgta tatgcggacg tttgcgtaat gctgcaatga taagacaaac aagaatatat
    39781 gaaagtcctg ccataactgc tatttgcata taaggtacca acctattaaa aaatgaaacc
    39841 ggtgtggtta ttgccagcac tgtcagcagt ataaaaatgc cgttgaagaa ttgcagatgt
    39901 ttctttttta tttccatggg ataaatttca tggaaaaaaa gtccaaagaa aagactgcct
    39961 aaataaaata cactcaattc cattttgatc tgcaactccc atgggaatcc cggaaatata
    40021 ttggcaatgg ttgtactgcc ggtagctgca atgcgggcac atactgttaa acaaaataac
    40081 ccaaaccaca gtggagccct gtccctggtt cggaaaagaa acaatgctat gttgtataaa
    40141 ccaatgatga ggattgcgcc aaacagaaaa agatcgctta tgactttagt gttgtagtgc
    40201 gcaagtattg ctctatcttt accaacaatg agggggtgcc acataccagc ggtgcgatag
    40261 tcccggttac tgatttgcag cacgcacaat gcagttttat tttttggagt aaaatatatt
    40321 gcctggtgcc tgcattcgtt acgcattgct gacagattct ttgacacctt cccgttattt
    40381 gcaacctcaa caccgttgat gtacaatcgg tacgctgtga ccatgtggtc taaataaatc
    40441 ccatagatgg cattatcagg aaggagtatc ttcagtgcat aggttgcaca tccttttgca
    40501 gggagcaagg tgccattgag cttgtagtcg gtccagtgtt gcggcatcat aacgatatcg
    40561 gtaggttgag gcgctttgtt gtcatcaata gatacagggt caatgatctg attccaccag
    40621 aatagccatt gtgctttaat ggcataggtt gttgtagtgt caaattttag attacgcatg
    40681 tcataggtag cctcagcgct ataagccaga gaagaacaag aaaaaatcat ggcgatagtt
    40741 actgatacaa gagtggtatt attcataatg gattatcctg taatacaata tttatatgat
    40801 attacagtat aatcaatact attgcaatag aaaaatgttt ttggtataaa ggcagctgtg
    40861 gttgatgcag actacacaag gttgattttt aatcctgaaa tgtgattgca ttactttata
    40921 ccagtttata tttccctgtc aagactgcct attcatagtc ttcagggcgt acaagccccg
    40981 taatggcttc tgatagcaaa aatcgcctga tgttttgcgc agctttttgt gctgctgtag
    41041 caagtgcacc atgcacaagt gctgaattat gaggtgtgcc aagcacattt ggcagatcaa
    41101 ggaagggata cttcatctca aaatgaccgt gtcgaaacgg ttcagtccac catgcatcaa
    41161 tcccggccat aaaggttgga gtatttttaa ggtgatggta aaatgctcct tcgtcgataa
    41221 tttccccgcg gcctacgtta accaaaattg aatctggttt catgagttta aattcacgct
    41281 cgccaataag ccctcgcgtt gcacggttta agggcaatgt gatgacaatg atgtcggcaa
    41341 gtggtaatac cttgcccata tcatcaggtg tacctaaaaa ttcagccgga tcaggacttt
    41401 tgccactacg gttaatggca aggatatgca tgttaaaagg cttgaaaagc cttgccacct
    41461 ctctaccaat cccgccaaaa cccagtatag ctgcggtggt gccggacaat gctctgtttt
    41521 ccgaaaattg gtcaaaaatt ccttgcttta acttttcatg atggatatag agtctcttgc
    41581 tcaaagccag caccatagca agaacgtgtt cagccattgg ttgggcgtag gctcctacat
    41641 tgcttgccac tgtcaggttt gctggcagac ggctgaagtc aagatgatca gcgccagcag
    41701 acaaaagttg cataaacttt actttttcca gcaaggtaat ttcagctggt gaaaattcac
    41761 tgtgtggtgc ccatgccagc acaacatggg atgtttgcaa caattttgcg cgttcatcag
    41821 cgttgaggtc ttttatataa ataatccttg caatgggaga cagaacagac tctatacgat
    41881 ctttttcttc agcatttaag ttaaagctta ttgttataag aggtttttca gtattgtcca
    41941 tatcattcct ccttcaagaa agatacctat gactatggca agatacccaa aatgctggat
    42001 tatcgattca aaattcattg tgaaccctta tactctttct tttcctctat ggcactcaaa
    42061 agttcctgta tgtaatcaat ttgtaaatta agaatttaaa aaagtttatt cacaaataat
    42121 catgccttat agaactttct tgtggaacat tattataaag atgtattatt ggtgtagatt
    42181 attcctcaga gaaatttata acgcagctaa ttttatgaat aaacattgtt cttattgctg
    42241 taaatccatt actatagcgt ttcatagaag cagtacattg aatatttttc tttttgaaaa
    42301 ttgaaaatct ataggtaaat aaaaaaatgc aaaaaaatat tttttaagga atataaacat
    42361 attcataatt ataatctgta tttgttgata gtaactatcg aacacaataa aacataacag
    42421 tgtaattttt aattgataaa taaatgcatg gtgattacta tgttcataaa tattgtaata
    42481 ggggtataga ctatggaata caaatattat ttagtagaga aaaagccgcc ggttgcatgg
    42541 gtctttttga acaggcctga aaagaaaaat gctatgaatc ctgcagcgtg gtgggagtcg
    42601 ccgtctgtgt ttaaagagct ggatgctgat gatgatatac gagttattgt cttagctgct
    42661 aaaggggcat cctttacggt tgggattgat ttgattgaga tggctggtga aataaaagag
    42721 cttgccgaac catcccagaa aggagggatt aagcgaagcc tgttacagaa aatttataga
    42781 atgcaggaca ccatgacatg cattgaaaaa tgccgtaaac cggttattgc cgcagtgcat
    42841 ggatactgca ttggtgccgg gctagatatg atagcctcgt gtgatatcag gctggcaagt
    42901 gctgatgcac agtttagcct tcgtgaagct gcggtaggat ttgtggctga tgttggtgtt
    42961 ttgcagcgcc tccctcacat tgttggtcag ggcattacac gtgagttagc ctataccgca
    43021 aaatatatta ccgcacagcg tgcaaaagag atatcgttaa ttaatgaagt gtttgctgat
    43081 caaaaggcac tgtatgcagg ggctgagaag atggctatgg agattgctga aaattcacca
    43141 ttagcggtac aggcatcaaa ggatgtatta aactttggta ttggtaaaac tactgatgag
    43201 gcattaaaat atgtagcatc aataagtgcc aacatcattc cttctgatga tctatttgaa
    43261 gcctttacgg catttgctca gaagcgaaag ccaaacttta ccgggaaata ataatgaacc
    43321 ctcattatcg ccctctcctt aaaaaaagga gagggagtaa tgatgaggtt tgatctaaaa
    43381 caacttttta agtttctttg tagctttatc ttttgctttg tcggttgttt tttctactgt
    43441 ttgtgatact tttttaacgg ctttttcttt agcaacatca acaacatcgc ctgccatatc
    43501 tttaataata tcatttaaag aaggaagctt tggttctaca agttttgttt taggtttatt
    43561 cattgggccg gttacagcaa atttaaggta aaccttcccg tctttattgg ttaagtattt
    43621 gaaggcttga tcagcaacat tttcaggttt tacatatttt gcaacatcac ccttgataag
    43681 tttagaagcg ttgcctttta taccgttata gatactctta ttgtgcttct cgttgaggat
    43741 catatcaatg tcggcattaa ctgtgctggt gttaagattt atgtcggctt tgaagttgcc
    43801 tgcataatca tcagtgctta ttttaccgtt agtgatcttc agcaatcctc ccttgtacca
    43861 tatatcaata tagccgtttt tccatttaac atcctttgcc aggaaatcaa atttcccggc
    43921 gtagtttgcc agtgtttcat tgctctttat agcatcaaat atctgtaatc ctgtcatggt
    43981 accatagggg cttcccgctt taagcgctat ttcaggattt aagatacgtg ttttaagatc
    44041 aaatggtttt acgttgcctt ttgcagatag ccctatatca aatgactcga cactcccggt
    44101 acttgtcttg ccaatggtct tgatgcctat gtctacggta agctttacct gatttttatt
    44161 ctttaaatct ttaggatcaa tttctatatt gtgcaccaat gcattgcaat tatagagcat
    44221 taccttttgc cctgatgatt gatcggtaaa ggtgacagtt cctttttcta tgccaatcct
    44281 gccaatggta atacttaccg gcaatgtatc tgctgttaaa ggttttgatg gttcttcttt
    44341 tttaacctct tctacttttt tctcttcttt tgtagatggt ttcattaaat cggagaaatt
    44401 aaatgcaccg cttttatagc gtacaatatt taccttcgga ccactgagta aaagttctct
    44461 tagctcaaat ttaccctgta acaatggaag gattttgagc ttaaaggtaa acgaatcaag
    44521 ccctgcaaaa agatcagctt tatcaacagg ttttcccttt aatgctgata gttgttgcgg
    44581 tgttttgaaa ttggatatag caacgccttt cacctcaatg ccagaaagca cactgaatat
    44641 actgacatca atggattcaa tggttacatg acggttaagg actttttctg cctgctgagc
    44701 aatcatatct ttgttgatga tgagcattaa aacaatactg gcaataataa ccagtgcaat
    44761 caatccacca ataactatcg caagccattt taaaaatctt ttcataactc cctccagtaa
    44821 ttatataatt gttgtaataa atgtattaat aagtatagta aaacatattt tggttacatt
    44881 cagagtaatt aactatgtat tgtaatatga tagctaacca ttctatatta gcaagcaaaa
    44941 attttcttga aaacaaatgg gatattttca acaataacgt aattgacatt tttgtttata
    45001 aatactgaaa tgaacaagaa atagtgatgc tatagcatag taattgaaat gaatagttta
    45061 tctttgtgag taaggtagta aaagcagtca ttgtataatt ttaaatatga tagttgaaga
    45121 tagtaattac tgtatagtgc aatgatgaga ataaacaggt gtaagggata cagtatgaaa
    45181 tcaaaaataa taattaccgt tagttttgtt ttatgtgttg ggtatgcact ttttcttggc
    45241 tgcaaatcaa cgcctcatta tagattggca cagtttataa gtccttccac atgtggtggt
    45301 tgtcatgatg agatatatga acagtggaaa aattcaatgc ataatttatc tcaaaaggat
    45361 gtcatctata aagagtttgc acgattttat ctggatgata gcaaaagtca aaatcctgtc
    45421 aataatgatg aactaaaaga ggcagaaagc tgtgtaaagt gccacatccc tgtgggatat
    45481 attactggta agccagtatc agtaactgaa gaattatcca tggagaagga cctgccggaa
    45541 ataaccatgg aaggtatcca gtgcgattac tgtcatacta ttgttgaagc aaagaaatac
    45601 tataacgcac gatttgtgta taaaccaggg aatggcgagg cggatcccgg gataaaacgt
    45661 ggtcctttta aggatgctaa tcctgattgg catacagcag aatattcttc gttccataca
    45721 aaagctagtt tttgtgggac atgccatgat gtacgccatg tggtgtatgg aacaaaattg
    45781 gagactacct ttgaggaatg gacaaaaagc ccttataaca ccggtgactc caaaacaacg
    45841 gtgccctgtc agggttgtca catgtatcac cgaccagggg tgcctgcaac tggctccaca
    45901 cctcgtcctg acaatcccgg ttttgcagcg tttggtggtc cacagcgtga tcatatcttt
    45961 actcattatt ttgttggcgg aaatagtgtc atcccttcat tatacaagga taccgtgaaa
    46021 gcccagatgg cacaggaacg gctgaaaaac gcagcagtag tggaaattga aaccagtgac
    46081 agtaatagtg gtacggttgg tattgctgta accaatattg gtgccggtca taagcttcca
    46141 acagggctta ctgatgtgcg gcagatgtgg cttgagatag ttgttgtaaa tcagtatgga
    46201 agagaggttt ttgcaatagg gaaggctgat aaaaaaggtt atttgcccga taatacaata
    46261 atttttaaaa ctgtttttgg tgatggtagc ggtaaatcag tgatgaatat tgcaaaagcc
    46321 agggagatac tctttgataa caggattcca cccaaacaaa aagttaccag caccattatg
    46381 ctgccaaaag gattaaaggg taactatact atcaaggtac ggttgctgta tagaattgct
    46441 gatcaaaaag cagttgatac ggtaatgaag gaaaacgctg tcaaacttga tattattgag
    46501 atggcaactg caacaaaaaa tattatactg taaggtgttg tggtaacggt taaagccagt
    46561 aatctttcaa aacgatatac cagaatatta ttcagggata tgtcgttttg tatagctaca
    46621 ggacaatccc ttgccattgc aggcccaaat gggagtggca agtcaacctt gcttaagatt
    46681 gttgcaggag tgatgacacc tgatagtggg aatgtggaat actggtataa tgataaacct
    46741 gttgatactg catatatcag aaactatatc ggttttgctg caccgtatat caatgtgtat
    46801 aaatatctaa cagcacttga aaatattgcg tttgcaattg gggctgtgga tgatgatgca
    46861 gtacagcttt tagatttgct gggactatcg ccccatagcg atacaaagct tatgttttat
    46921 tccaccggta tgatccagcg tgttaagtta gcgcttgcat ttttaaaaaa agccagtgtg
    46981 ctttgtttag atgaacctgg tagcaatctg gatgagcagg gtaaaagtgt gcttttttct
    47041 ttgctggaga aggtacagcc agcaacggct attgtcattg caacaaatga tgtccacgag
    47101 ttgaactttt gtcaaaggag tatagtgctt gattaaggca ttatggcgga acatccagaa
    47161 ggatatattg attgaattca gaagtgggat tatcgtttca attgttttat cattcagtgt
    47221 cattgttact atttccattg gatttgcaac aggtggtatc atcaatagcc ctttactgca
    47281 tgctgttata ctatggataa tcattttttt ttccgggatg aatacactat cgcatgtttt
    47341 tttcagggag atagaggagt caactgcact gtttggtgta atacattatc cggctgctgt
    47401 tatctatctt tcaaaactct tgattaattg ttgtgtaatg gttgttattg ccattatggt
    47461 catcattatc tatacttttg ttatctcagt ttctgtgtat tacggtgtct atcttatagc
    47521 aatactcatt gcgggaagtt tatcgcttgc ttttgcaaca acggttatag gagccattgc
    47581 agcattagcc ggcgcacggg gaggattatt taccatattg gcttttccgg tagtattacc
    47641 gctttttact gttgcaatac aatcaacagc caggtgtttt caacagcagg ttttttctcc
    47701 attaagcgct ataatttttc tacttgcatt ttcaatagtt cttggaatgg tatcatattt
    47761 attatttcca tatatctggc gcgaatttta atagtaacga gaatagctat gattgtaata
    47821 cttttaaatg gcattgtcat gatgctatca ttgtatttaa tctatatctg ggtgcctccc
    47881 gcggctatat taggagaaac aagccgtata gtatacctcc atgtgccact tgcatgggta
    47941 tcttgtttgg cttttgcagt agcaggtatt gcttcactat tgtttttgaa aaaatcaaaa
    48001 ataaactata tagtgtatgc acgaagttct gcacacatag gtcttttttt tagcatcctt
    48061 actgtaatta ctggctcgtt gtgggcaaag gttatgtggg gcagttactg gaactgggac
    48121 ccgcgtgaaa cctccatcac actattgctt ttattgtata ttgcctattt cagcctgcaa
    48181 cagattgtta gcggtaatcc aaaagcccct atgttagaaa gtgtgtatct tataattgcg
    48241 tttgttgtat caccgttttt aatgtttgta gcaccacggc tttacccaac gctgcatcca
    48301 gaacctgtta ttaatgctca gggacaattg atgctggata ctgaaatgcg gttggtgctt
    48361 ggtatatcat tggtatcatt tacgcttttg tactacatat tgcttggatt gtgggtgcgg
    48421 attcatgtac tgaaatcaaa attgcacgag gaatagtatg aaattatatg cgatagttac
    48481 cgcaatgcat tttttggcgc agacagattc aaattattca aaaacgcttt tctgggtatc
    48541 tgcggttgta tggtgtggaa tagtctgcta tcttgttgta ttgcaccgta aaataaaaca
    48601 gctggaaaag agggtcaatg gcaaaaagta agatcattat tgtggcaatc attgttatat
    48661 gttctgctgt agcacttttt tcgtttaagg ggcttttaac gccgtatgtt ccctttgatg
    48721 atgcaaagaa taatcccgat tatgttcaga taattggcag gctggttaaa acagtgccta
    48781 tgcagaatac cacagatggt tttctatttg tgatagaaga taatgcattg gaacggatga
    48841 cggtactata caggggtgcc aaaccgttga actttgaaca tgctaccagc gttgttgcaa
    48901 tcggcatcta taataacaaa aaacagatgt ttgaagcaga taaaatattg gttaaatgcc
    48961 catcaaagta taccagggag caaaaataga tgaatagttt atattatctg ccatggatta
    49021 cggcactatt gattatgcta tcgacaatgg cagctgtata ttcattgagc aaaccaaaaa
    49081 tatttacttt agcaaaatat ctttattatg ctgcaacagg gattgtagca ttaatgctta
    49141 tatgtttgtt aatggcattt gttaccttta aatttcaata ttcttatgta taccactatt
    49201 catcggttga tttgcccgta ctataccgta ttgccggggt atgggcaggt caggaaggtt
    49261 ccatcgtctt atggacattt tttataatga ttgccggggt tattgccgtt accagggaag
    49321 aacaattgaa acaaggcttt ttaacaatag tgggtttagt atcgcttatc tttgttattc
    49381 attgtatcaa taataatccg tttacctata tatgggatac atcaaaaaat tttcccgtaa
    49441 atataattcc cacagatggt gccgggctta atccactatt acaggatttc tggatgatca
    49501 ttcatccacc ggttcttttt attggctatg cattatctac catagcgttt atttatgcaa
    49561 tactatcgtt actatataat aactattcca ttatacttac cgcttaccgt tatgtgctcg
    49621 caaccgtgtt gaccctggga gccgggatat ttttaggtgg ctactgggca tacaaggtat
    49681 tggggtgggg tggttattgg ggttgggacc cagttgagaa tgcatccctt ataccatggc
    49741 ttatcagtat tgcactattg catggattaa tactgcagaa acgaaaacag gttctggtac
    49801 gggctaatat tgccatggca ataggggtat atgtttttgt cctttttggc acatttttaa
    49861 cacgaagtgg ggtgctttca gatttttcgg ttcattcatt caacggggca ggcaatttta
    49921 ctgatctttt gttgtttttg agcacgacag ttgtattaag cattggcttg cttatctatc
    49981 gctccaggga tgcacgtggc aaagttcttg ataatgatgt gctttcaaaa gaaaatataa
    50041 tggtatacgg tattgctgca atggtatttt ttgcattgtt tgttattatt ggcacctgta
    50101 tgccactgct taccagatta ctatcgcaac catcaaccgt tagagaatcg tattacataa
    50161 gcattgtaaa gccagttggc ctggttatcg tgatattatt actgcttgct accttcacaa
    50221 aaaaattacc gttgaaagca aggacttttg ttataccgat aactatcgcc ttaactttaa
    50281 caatcctttt atatattggg gagacaatcc gcatattccc attagtatta atgtttgtaa
    50341 gcttttttat tattgttacc actataaagg gcttgtttcc ttatacccat ttaaatagag
    50401 ccgcacgggt agcccacagt ggcgtggcac tgcttattat tggtataatc acatcacaaa
    50461 cccagtcgct ggattatcat acggaattaa tccagaagga gaaaaaaacc atttatcctg
    50521 tttcacttga gcttgtaggg atgacaggtg gcgctaagaa tgctctggtt tttcattata
    50581 gttactacaa caaaggcaaa actattacca caccttattt tattgatacc agaacaaacc
    50641 agctattcag ggagccttac attgactaca atatactcta tgatgtgtat atttcacctg
    50701 ttgaatatat gagcgggtat gataagctga ctcaggtgat gctcaaaaaa ggtgagatac
    50761 gtacgattgg tgatatcacc atacgttttg ataactttga tgtcgatagg gcagccatga
    50821 tgaaagggaa tgccattgtc tatgcacaat gtaccgtcac ccataagggg cgacagtata
    50881 cggttaaacc tggcgttgcc tataccaatg ataggcgaca ggatatcaaa gctatgatcc
    50941 ccggaacaaa ccgcactatt gttctgcttg attttgatgt acgggatgcg atagttgttt
    51001 tgcacattga tgctcatgac aatgcagttc ttcctcctga ttcggttatt gttgatatat
    51061 cgtttaaacg acttatctgg cttgtctggt taggtacggt gataatagtt gttggtgttg
    51121 cattgccgtt tgtcagacgt agttgagcat aggctataaa ggaaataatc atgtcatcag
    51181 aaacggaaat agtacagaat aaaaaggcac gctttgagta tgaaatactt gatactatag
    51241 aagcaggtat agtattgaag ggcactgagg taaaatctgt acgcttaaaa aaggtaaaca
    51301 ttcaggactc gtatgcccgc atcaaggatg gcgaagcttt tctctatggg atgaatattt
    51361 caccgtatga gagtgggaat atctttaatc atgatccgtt acgggtacgc aagcttttgc
    51421 tacataaaca ggagataaaa cgtcttaccg gcaaacagca ggaaaagggg cttactctgg
    51481 taccgttaaa ggtatactgg aaaaatggca aggttaaggt gctgcttgga cttgctaaag
    51541 ggaaaactct ttatgacaaa cgccatgata taaaaaaacg tgactcggat agagaattgc
    51601 agcggtataa gcgcatgcgg tgattatgta taaagagcta ccgattcaat ttttttattc
    51661 ccattcaatg gttgatggtg gttttgatga gatatcatat acaactctgt tcacgccttt
    51721 tacttcatta ataatgcgtg tagagattcg gcgcattaaa tcttcaggca gatatgccca
    51781 gtcggctgtc attgcatcaa gtgaggtaac tgcacgcagt gctatgacat tatcgtaggt
    51841 acgtttatca cccatgacac ctaccgattt aacgggtaaa aataccgcaa aagcctgcca
    51901 caatgtgtta taaaaatgag cattcttaat ttcttctata aatatgtcat cagcttcacg
    51961 cactatggca agacgttctc tggtaacctc gccaatgatg cgaatagcta aaccaggacc
    52021 ggggaatgga tgcctgttaa ttaattcttc gggcattcct aattgtctgc ccaattcgcg
    52081 aacctcatcc ttaaatagct cttttaatgg ttcaatcact ttgccttcat tgataagctc
    52141 cagcacttca ggcaccctgt tgtggtggct ttttattgta tcagatggtc cctttgtaga
    52201 aactgattca atgacatcgg gatagagtgt accctgtgct aaaaagtcaa aggttccaat
    52261 tttagcagct tcttgtttaa aaatagtaat aaattctttg ccaattattt tacgcttttc
    52321 ttccgggtca ataacgcctt taagtttatg taaaaaatgt tcttcagcat cagcatagat
    52381 gagattgagt tttagatgtt ttttgaaacg ttcaataacc tttatgtgtt cgtcttttcg
    52441 taaaaggccg ttgttaacga aaacacaata taagcgatca ccgatagctt tttgtaacaa
    52501 cactgcggta acagaagaat caacgccgcc ggacagtccc agtaaaacag ttccattttt
    52561 taccatagtg cgtatatcat caatcgcagc atcaatgaaa gattgcatgg tccatgtagg
    52621 ctggaaattg cagatagtaa ataaaaaatt acgtaaaatt tcctttccgt actgggtatg
    52681 atacacttca gggtgaaatt ggacaccgta aattttcttt tccctgtttt ctatagcagc
    52741 gtgtggtgca ttctcggaac gtgctatggt tttgaatgtc tcgggaatac gttctacggt
    52801 atcaccgtga ctcatccata ctgtttgcgt gcggggcaga tccttaaaca atggagattc
    52861 ttcaattaac gttatggttg cccttccata ttccttgaat ggagaaggat tgatgatacc
    52921 atgaaatgca ttaacgacag catataaccc atagcaaata ccaagtaccg gcaatcccag
    52981 tgaaaacaga tcatctgaga tgtgcggggc gttatcctca tatagtgagg aaggcccgcc
    53041 tgaaagcaca atagcaccgg gattttcttt ttttatgtcg gcgatagtta ttgtataggg
    53101 aactatctct gcatatacat gcaattcccg tattttacga gcaatgagct gtgtgtactg
    53161 cgaaccaaag tccagtatga gcactttatt ttgagaggat attccnnnnn nnnnnnnnnn
    53221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53281 nnnnnnnnnn nnnnnnnnnn nnnnntgagg agtacggtga gcagaatacg cttttacatc
    53341 atacgaaaca ttaaaatcat ctaatgtctg gaaacacccc tttaattcag gaaggtccga
    53401 atcactgcca acgataatcc ctatttctgt catatattcc tccatggaaa ataaatttat
    53461 taccctcacc ccctgtctct ctccttcgta aggcgaggaa tgaagtgtcc tcataaaata
    53521 ggtactatac acccctccca tactggactt gatccggaaa ctctttttca ccatgagatc
    53581 ctgaatcgga gttcaggatg atatttcttg ccattgccat gattaatccc ggactatcgc
    53641 cagggcaggc ttatttcagc atctatttca taactgatat cctgaaacaa aactgtcatg
    53701 gaattgatcc atagtacagt ataagcacaa aggaacctct aataattata aagtgaaagt
    53761 atttttaatt tttagagtta cctctttgat atgatgaaaa acttacttgc atataatgcg
    53821 ttatattata cacataacct cataagttga aatgctatca tatcatatca ttttatgcta
    53881 ttacagctat aatgcttgac ttaaaatata actcatgtag taataaaatc aaatgtaaaa
    53941 tatgaaaaat ctatgtcatt attaagcatt acatttttta tagttggcgc atcaattgtt
    54001 ggcgcgctgc tggtaattat acgggcacat caatatcctg caaaagtgcg aaaagcccaa
    54061 gaatatcttg ataatggaga cattgcaaaa gcaagtgaaa ttgtcaagct ggttcttgaa
    54121 cgaaaaaagg attatatacc agcgcgttat ctcagggcgc agatattaat tcaacaaaaa
    54181 cagtatttac ttgctatcgc agaacttaat ggaatactca gtatacccga ttttgataaa
    54241 tatgtagagg aactgaatat ccattatcag cttgctgatc tataccatga aacacaacaa
    54301 tggcaaaaag aggttgaaga gttcagagtt attttacagt ttaatcccga cgatatcaat
    54361 gccaatcaca gattagggat tgtgctttac cgtcagcatg attataccgg agccaaagat
    54421 tatttattga aagcagttac actggaccct gctctttctg atatttatgc accgttaggt
    54481 atatgtctgt ataatacagg tgaatatgga acggcagaaa attttctgat taaagctgtt
    54541 gaaacaaatg ctacaaatta tgaagcgcat tattatcttg gtatgatctt taagatgaaa
    54601 aaagattata ctgctgcttt agagatgctt agtaaggctc gtaacgatag acggtatttt
    54661 gataaaagcc agtatgctat aggacagtta tattttgata atgatgcata ttctaaagca
    54721 atcgaagaac tggaaaaagg attgccatat attaaggata atacagacga aggattggca
    54781 tatcgttatt tattggcgga atgttatgag atggaaaata agataccaga agcagtacat
    54841 cactgggaaa agattgcaag tataaatcct aattatcgta gcacacaggt aaagcttgac
    54901 gattacaaat taattcttga aaataaaaat cttaaatttt tgtttacttc tactatggca
    54961 gagttacaac cacttattac tgagattatt gctcagttga attatagtat agtatataaa
    55021 aaagaagttt cggctaatga gtacatgtac aaatgtttta gtgttaaaag gataaatgaa
    55081 ccgccggtct taattaattt tataagaaca acccgtgata taaccgaagg gcagattaac
    55141 actttttatc agcgtatgct tgaagagaag tgtaaaagtg gtatttatat aacaacatca
    55201 aaatttagta ttaatgctaa atctcaggca gcaaataaaa tgatagaaat catggatggg
    55261 cgatttttac aaaaggcaat ggaaaaaatt aaaggtaaaa aaggttaaac atgattcttc
    55321 gttacctttc aacgtgcact attgttcttt tacttattac tctatcatgt tcaaggcaaa
    55381 aaacggtaaa tgaaggaatt gaactgtatc aggaggggtt atatcaaagt gcctttataa
    55441 aaattaaaga cgggctaata cagtattcta tggaaaaaac gttggatgag caaagttttg
    55501 tttgtactcc atatgcagtt ttttctacag agacattact caccatgctg tacccaaaaa
    55561 aacatacata tgaattccca cgtgaagtag ttagtgtatg gtatgatcct gccgatgatg
    55621 tttgtgttgt aactgatggc attatggtat ctaatttttc ctcagatgag gaaatcatta
    55681 tcaataaccc aacaaccaaa cccattaaag cagtttcgta ttgcaataag gatatttatt
    55741 tgctctgcgg ggaagagtta tatgtacata gccaggatga taaaaaaata agaagattgt
    55801 catctataga tttcccacca ccggaaacat tgaaatacta caatggatat atgcatgctg
    55861 tcaataataa gctcatcata attgtgggaa tagccggaat gtataatgca tgggtgttat
    55921 caactgaagg ggagattttg ctatcccgta tacggattgc ctcatggaag aatatactaa
    55981 aagatgatat gctgtttgtt attaccggta gtgctggaca ttgggattta aaaagtatca
    56041 gtatcacaac gaaaaaggac aggacaatta aaagctttac aaaactgcag gatgttcatt
    56101 tgctcaatga tggtattatc ataaacaacg gcgatgaaat agtagtttat aattatgata
    56161 atacatttta tgcaatacca tttacttata g
//
LOCUS       DAOOJH010000009        54131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_709_length_54131_cov_9.394926, whole genome shotgun sequence.
ACCESSION   DAOOJH010000009 DAOOJH010000000
VERSION     DAOOJH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 54131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54131
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_54131_cov_9.394926"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PLJ75_02395"
     CDS             <1..378
                     /locus_tag="PLJ75_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04102.1"
                     /translation="ENYQMGVEWFVIDGLAIGGGISYNTWDEAETEVFTVMPTVTYYY
                     AMDKLIPYIGAGFKYIDISDDPALGDGDGYSIFGKLGVAYMLGNNLALFAEFQYSYDT
                     LDLDAGDEDGNVMSGSLGVKAFF"
     gene            578..1711
                     /locus_tag="PLJ75_02400"
     CDS             578..1711
                     /locus_tag="PLJ75_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HPD04103.1"
                     /translation="MPAFVHISDTHLGFNDLDRVDDNGINVREADVYGAFEHAVDRIV
                     AMKPDFVLHTGDLFHRASPSNRALIQGLNHIKRITQAGIPFIVIAGNHCTPRTIYTSP
                     ILQALQTIEGVFAVYSQRYEYVTIDGIHIHCLPHIHDDMRYVEQIRTIEPKQGCNILL
                     MHTSVGQDYLLDEYGERIFPAEYYHLFNAFDYVALGHWHNFKVLPPWDTVCYSGSTER
                     FSDREAGREKGFLTVTIAQNKNITFNAIPIRPWVRFDITRCSELPIDDIKTRIQHFSS
                     QVEDGSIVSIYLHDVDTRQSAGISSVWITEQFKNAMAVIPKRIFKKQGGDSYFQSEHV
                     PVDELFVQYCTNAIADEKERALLVASGRRYFKNYYDRVDEKRE"
     gene            1715..>2270
                     /locus_tag="PLJ75_02405"
     CDS             1715..>2270
                     /locus_tag="PLJ75_02405"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPD04104.1"
                     /translation="MIIQSLTLVNFRQYRDETIEFKEGLIGIIGKNGSGKSTLFNALV
                     CALYGELPSMKEHIRSSFVQPNETVSVTVCCEIENSDYKIIREFKGKNLTPYAALYKN
                     GNEIAVGATDVTRTIERIIGMPKEAFTRSVFAAQGELREISESRGGQRRELIRKMMGF
                     QMLDDILQTIRDDKNKMKNVIDGQQ"
     assembly_gap    2271..2370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2371..4272
                     /locus_tag="PLJ75_02410"
     CDS             <2371..4272
                     /locus_tag="PLJ75_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HPD04105.1"
                     /translation="QTIRDDKNKMKNVIDGQQAMLLLDSERDEKKAALKSLYDELSNI
                     KKEYDDVTRTHDNMLQQFSNVQKEFEKQQKIFKEYTVLQQQLTQEKTRLENCIKNISE
                     TEEALKQLYKASDEFHALHEQEVKFNTVRKQKDELDTVRVRDAQKKQLHQQFALIEKQ
                     IHEKSELLQAESNEVLEYETILQQLNNALKDKSKIAQALQAFHNKKNDISSTIGGIQK
                     TIKDRQKHIIQIEKLGREAECPVCLRPLHDAYDATIQRLTNEIEEYERREMASLQHAL
                     QTVTSNIAESEKQLSKTDELIGTLQQKKAKLEEKQNTIKKIKKEIDGQQQSREDIRMK
                     LDDLSSVSFDEAVYNDIVQQYHKLEAMHTRYLALQEKINGIPESEQKKAALNKEKEAL
                     HAVIHDVTKHIEALHYSEHAYEAVKNEFDAMAKTKDSIVQRLNDVHLQLTNHNNKITQ
                     IEHELQKDDENRHKVEKARNELNIVLQLEQLMDGFRTQVLQFIKPAIAQYAGNLFNQL
                     TQGRYQSIDMDEDFNFFIMDDDVQYPLSRFSGGEIDCANLCLRIAISNAIRDFSGGGA
                     VGFMAFDEIFGSQDNDRRAAIMDVLYRLQEHYRQIFIISHIDDVKELFPSILHIQNSG
                     TGSTATWLA"
     gene            4843..5769
                     /locus_tag="PLJ75_02415"
     CDS             4843..5769
                     /locus_tag="PLJ75_02415"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD04106.1"
                     /translation="MVTRKSFLNNIKDLLMAIPVAGLPWLKNIYAKDMVSSRDLVVVK
                     AKTITDASIAAMVKVGLQEMGGIERFVKRGSKVIIKPNIAFNSTPDRAHTTNPSLVET
                     VARICKNAGAQVTIMDRPVHNARLCYHSSRIEEAAKRAGVNCEYMNRDRFKTVHVPQG
                     LNLDTVDVYEEILEADCIINMPIAKHHNAAMVSLSMKNLMGVIGGNRGFYHVNLHRNI
                     VDFNKAVKVHLIIMDGLRILTKHGPSSGGPEDIKETKTIIFGINPVMVDTYTAHYLFG
                     IEPSSIGYLSLASNAGMGSINVNTYSVITKTV"
     gene            5766..7259
                     /locus_tag="PLJ75_02420"
     CDS             5766..7259
                     /locus_tag="PLJ75_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD04107.1"
                     /translation="MKSKHWSLFRAIIQLAFWLIFVGSIVYLHDPLSDVRYGLYPSLS
                     IYSIFIGSITGHTLKYAFLGIAIALLTVLFGRFFCGWICPLGATIDASDTVIETKAKN
                     KQLKKITRIKILILLATLLIAFLGFNATGYSDPITIAYRGYELFLYPFISYLGSEPLS
                     WVLPGYAARDYPFYYYNWIWPAIIFIGILALSLFFKRFWCRVLCPLGAIYALLSHFSL
                     LRRTVDADKCVHCGACVKGCRMGALSSDGTGTIEHECIKCFDCLKHCNYGAIQFRFLP
                     PYKSKSIAHPAQGITRKDLLVVAGSSLMIASTRLTLFAASHESYIIRPPGALPESDFC
                     NACIRCGQCMNVCPTNALHPCMIEAGFYGMFTPRLVSRIGYCDFSCTRCGTICPTGAI
                     QKLSLAKKQTFVIGTAYIIKDLCIPWSESTNCIVCEEVCPVQNKAITLDRKVITNKKG
                     EAVEVLLPVVHTELCIGCGICENRCPVSGGGAIMVRKPKLTMGDSYG"
     gene            7252..8160
                     /locus_tag="PLJ75_02425"
     CDS             7252..8160
                     /locus_tag="PLJ75_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPD04108.1"
                     /translation="MANEKHHEAYVARKVTWIGMIVNIMLSVFKLVAGIIGHSQAMVA
                     DGVHSISDLTTDAAVLIGIRYWSTPADEDHPYGHERIESLVTAFVGVALFIVGIGIAY
                     NGIVTAFEPDIKSPELIALIAAFVSIVSKELLYRWTVKLGKEIDSSSVVANAWHHRSD
                     ALSSIPALLAVGLAMIHPKLAFFDHLGAVVVAIFIIKVSIDIMKPVIMELSDKGAPKP
                     QISQIDSIVMGVEGVKEVHSIRSRRCGSGYFVDLHVLVDGNMSVQSGHDIARTVKYQL
                     LDKGPKVLDVMVHLEPYQTSSKKNST"
     gene            8157..8933
                     /locus_tag="PLJ75_02430"
     CDS             8157..8933
                     /locus_tag="PLJ75_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPD04109.1"
                     /translation="MKEYSVTIRFYEELNDFLPANKRKVAFTVSWKGKRSVKDLVESL
                     GVPHVEIDLIVVNGVSVGFDYIVQNDDYISVYPVFEQFNINHITQLRPHPLRHPTFIC
                     DVHLRKLVKYLRLFGFDVLYNEQWDDKDLAQIAAEQKRILLTRDRQLLMRKIVDRGLI
                     VRNILPQKQIVEILNRLDLWDMVTPFSRCIMCNNHVRQLLINDHSFDNEKKKIPIGVL
                     QWCTVYYKCLGCGRIYWEGSHYERLTKKIKTILSEKNKRS"
     gene            9221..10858
                     /locus_tag="PLJ75_02435"
     CDS             9221..10858
                     /locus_tag="PLJ75_02435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HPD04110.1"
                     /translation="MKILLGYCVEKEHYHDYYVTLLPVGLVSIASYLSNSGYDVVLAN
                     FSKKSTEQIIKEITHIKPQLIGFSLFTHNRSITLYIVNKIKILFPNSVIVLGGPHATF
                     LDKEILQRFPSVDCIVEGEGEAAMLAIIKNIEQGKPPRHEIINMHSVNDVSNIPFPSQ
                     FNGTLYNINVNEQYKYIITSRGCYNNCTFCDSPAFWGKKVRFREVDDVIEELETIYRK
                     YGIIYFSIRDDNFTVKKDRVQELSYKMIRKKLYMMWNCQARVDAVDESMLIAMKKAGL
                     EHIQYGVESGSEKILALYNKKTTIDTIIHAANITRKVGIYLSIYLMVGMKGEDYNDIK
                     KTKKLLASILPGDCIVSPVALYPGTKLYEEEKKNGALSDSIWFNKNDAGIFLRNEPVI
                     KEWMNELLMATYALRDKAWYKAKDFAMHKKIAGDCWVTDILEGDYYSDNELYPEAATV
                     YGNVIKNIPDNPWGYMRYGKLHFIIGDYETAMKSFLEVTKIVPNYYGGWLKFAQSCIA
                     LGLNEKAKQAINKALQLNPYDERINALNSLLKNNRRK"
     gene            10973..11944
                     /locus_tag="PLJ75_02440"
     CDS             10973..11944
                     /locus_tag="PLJ75_02440"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPD04111.1"
                     /translation="MAQNNIHDKAYKYLFSNPLIVKELLESFVAMDWIKQVNFNKAQP
                     IDKSYVNDDYKEYEADIIYKLYFKDRQLYLYLLIEFQSTVDRFISFRMLNYVMELYRE
                     LIYKQKLKKLPVVFPILIYNGDRKWTAPQSLQELIDMPESLKQCTAYIPQFQYYPIIE
                     NELSRKTLESMMNVISTVFLFETGDTMVFLKTVDRIKELLHIYNDEPLMLRTLFYWLM
                     HYLQTHGKIDDAAVILETIEEPEEVHSMLSKTIQKMQEDFRKEGFHEGIHIGIDKGIQ
                     KGINEGISIERRNIAINMLKKGIDKKTIIEITKLNKKQLADIEKGMQ"
     gene            12043..13326
                     /locus_tag="PLJ75_02445"
     CDS             12043..13326
                     /locus_tag="PLJ75_02445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPD04112.1"
                     /translation="MIANKNQVAAINAEQGAHLVIASAGSGKTYTIIQRSIALINKKL
                     CNPDEILLLTFSRKAADELKQRIVAVLGNAGSRVVAATFHSFCLQFIIKQTMNDIKVL
                     DEDISQNIMKEIINNHIAELYGIPSMVVYKIVSKLQTNRISQLHGDLYAKMIMIKEEY
                     ASYKRNHNYIDFDDMINISSKLLHENPLLRKKIHEQFKYIMVDEFQDTSENNFVLLKL
                     LLPEDKQNVFMVGDDWQSIYKFRNAKIEYIVNAKKYFNNLTVHSLNSNYRSKKEIVTL
                     SNRLIAKNKFRSRRWIKAVRGNGGLILFHKASSFAEEVTITASILEKYDSTHLIGVLY
                     RNNWQGTYLQSQIQSYGNVQFMTIHSAKGLEFDVVVLCGVKDRLLPDPYTDIEEERRL
                     MYVALTRAKNCLHILYHCNYGNQKPQFVMECERCF"
     gene            13408..14292
                     /locus_tag="PLJ75_02450"
     CDS             13408..14292
                     /locus_tag="PLJ75_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015281936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04113.1"
                     /translation="MDYILEVNNLYKGFPGVKAVDGVSFSIPYGICFGLLGPNGAGKT
                     TTIEVIEGIQKPDEGIILYKGKNRDHRFKQEVGIQLQATELQQYLTVKEILLSFRNLY
                     AKRADLNDLIAMCYLDEILNRDNRKISGGQKQRLLLAIALANDPELLFLDEPTTGLDP
                     QARRHVWDIVQTVKMKGKTIILTTHYMEEAQNLCDVVAIMDQGKIIAMDSPINLLNQY
                     CSGVTITLPSVIDVDMLNSIECNWYMVNNTIEIQTEKPHDCLRVLIDKNIDITGMVVR
                     SQNLEDLFLKLTGTQLRA"
     gene            14299..15327
                     /locus_tag="PLJ75_02455"
     CDS             14299..15327
                     /locus_tag="PLJ75_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD04114.1"
                     /translation="MLYRIWTIFVARTKEFYRDRSALGWNVIFPLLIIVGFSVVFRSD
                     SSPILKVGIVALAEKPAENGISNIQFNEFLKIKYINFIQFNSKEKAIGSLQKFKIDMV
                     IDSNSKTYWINESSPNGYLAEQLLKGAQNKSSVFKKQSFYNKGVQYVEWLFPGILGMN
                     AMFNSLYGVGYVLVMYRKNGILKRFSVAPVRPFEFLSAQILSRMFVVLATTVVVYVGT
                     VLLYGFKCKGSLFTLFILFALGSFCMISLGLLIASRSDSQEFADGIINIITWPMMFLS
                     EVWFSLEGSRPWVQKVSQFLPLTHIVDGARMIMNEGATLFDLQLQIVYLVAISLLFLF
                     LGSLLFRW"
     gene            15442..15513
                     /locus_tag="PLJ75_02460"
     tRNA            15442..15513
                     /locus_tag="PLJ75_02460"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15474..15476,aa:His,seq:gtg)
     gene            15526..15599
                     /locus_tag="PLJ75_02465"
     tRNA            15526..15599
                     /locus_tag="PLJ75_02465"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15560..15562,aa:Arg,seq:tcg)
     gene            15620..16261
                     /locus_tag="PLJ75_02470"
     CDS             15620..16261
                     /locus_tag="PLJ75_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04115.1"
                     /translation="MNSTVKDFDIFNYLPKEEDSVINNPYLQEILTYRNNQFDEYQYW
                     NVRYDLIKKYAFAVPTIDTIKIIQELSPIIEIGAGNGYWAYILNQVKVDIIATDKYPP
                     DETALPFSNYSSNQWFHNQWYDVIKAEAIEAGNHPERALMLCWPEPQSMMAYESLYAY
                     KNAGGHTIIFIGDMIACAESNFYDALAQCKLEVKQQIWGYKYFNEIIAIYKIY"
     gene            16347..17081
                     /locus_tag="PLJ75_02475"
     CDS             16347..17081
                     /locus_tag="PLJ75_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPD04116.1"
                     /translation="MIEAHKKILIVSMPMVDSDLKLYLAPSRCIDSDDPLVVDFAHSA
                     IGSEADAVKKSVKLFYAVRDNIYYDPYSPFYKPEHYHGRFVLQRGRGFCVSKASLLCA
                     LARACNIPSRVGFATVKNHIATKQLLETLGTNVFAYHGFTEFYLNKKWVKATPAFNKE
                     LCILHKVSPIEFNGVDDAIFPEYNSENKLYMEYLDYHGSFYDVPVETIITAWKECYGS
                     ERVELWITLFEQNFANRKFEGEEIVK"
     gene            17085..20597
                     /locus_tag="PLJ75_02480"
     CDS             17085..20597
                     /locus_tag="PLJ75_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04117.1"
                     /translation="MQIVPIIDILKFIDCDFICVDSLLEIIHSNNDSFINKNIADTFP
                     YSLIIDDVKRYCLELSTNAGVKTVVKFQNENCLYQFSLFDSKYGDKTIMVIIAKPKKQ
                     IPEVIQDICHNLFKVKIADDLQHFVDVFEYILNSIIQVTGMDCGGIYIIDQLTMKLKL
                     TFARGLTNSYIKSASIYNNESWNYQLVMNGTPQFIDYTNMELCDNDFRRLEGIKAVAI
                     VPIKFRNSIIGVINVGSHSTNTISQNIYPLLEVFGEQLGTIMDNYMVKSVLAQSKYKK
                     EIIDTIHDVVFQLDKDGIVQYMSGNISTITGFTPDEILGKYFINFIYPEDVAMFIESC
                     ELAKRRIFEPLEYRLITKEGSYRYILTISESHYDEKGNYIGLTGVVTDIHDKKLAEIK
                     LKESDERFKTLASSVAAGIFVINDFRVYYVNPAVIQILEYPEEEILGKLFWEFISEED
                     RGWIIERAKARLEGKSVPTRYELRLVTRNNTIKWVDFSATQIMLNGKPATLGSVFEIT
                     EKKRIESELKQSEEKFYKAFHSSPIPMTINTYNDGMYREVNEAALTTFGFSLQEALGK
                     SIYDLDVFADKNELKQYLQELRTNREVKDMDILFQTKNGKLKQCSINSDIFISSKEEF
                     VVSLIVDITQIKQNAQIILDQFNELQKINADLANVQAKITTQNIQLSEEKELLNRILE
                     SITDAVIVTDSYGKIVLVNTITCSILGKPANLILNTMASDLLTIECKQSVSLNFKEIY
                     DTLKNNSIHQECILRVNERKYDIEFNLLPLKNQHNDVNGAVILLRDITQKKIVEQQMA
                     KTSKLESTGLLAAGIAHDFNNILTSIVGNLSIVKYKINENSELYNSIRGAETAAFKAK
                     ELTQQLLTFAKGGSPLKKLTSLKNILVETSTFVLRGTAIEFRFIIADDLYNVEVDEAQ
                     IGQVIQNIVINSREAMQGYGVLKIAAHNVIENDPVRVIIGNELYVQISIEDNGVGIPR
                     DILEKVFDPYFTTKPHGTGLGLAVVYSVIKQHGGYIFVDSEAGKGSRFEIYLKASKNK
                     ATLTEIPYCDSITVTGCGKVLILEDDSNIQFILKTMLEELGFEIDITIDGEEAFYKYM
                     NAKYNNNKYAFAIMDLTIPGKQGGKDTIRQIRAIDKDFKVIVSSGYSNDPVMSNYSEY
                     GFNAILPKPYRLEDLKKVIATIMA"
     gene            20783..20855
                     /locus_tag="PLJ75_02485"
     tRNA            20783..20855
                     /locus_tag="PLJ75_02485"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20816..20818,aa:Lys,seq:ctt)
     gene            20924..21005
                     /locus_tag="PLJ75_02490"
     tRNA            20924..21005
                     /locus_tag="PLJ75_02490"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20958..20960,aa:Leu,seq:tag)
     gene            21010..21081
                     /locus_tag="PLJ75_02495"
     tRNA            21010..21081
                     /locus_tag="PLJ75_02495"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21042..21044,aa:Gly,seq:gcc)
     gene            21396..21468
                     /locus_tag="PLJ75_02500"
     tRNA            21396..21468
                     /locus_tag="PLJ75_02500"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21429..21431,aa:Thr,seq:tgt)
     gene            21474..21555
                     /locus_tag="PLJ75_02505"
     tRNA            21474..21555
                     /locus_tag="PLJ75_02505"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21508..21510,aa:Tyr,seq:gta)
     gene            complement(21680..21889)
                     /locus_tag="PLJ75_02510"
     CDS             complement(21680..21889)
                     /locus_tag="PLJ75_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPD04118.1"
                     /translation="MKTSITKDMTFGELVQKYPVAASILASYGLHCIGCHIGIYETIE
                     QGSRAHGLTDAQINEMLEKLNNTIK"
     gene            22171..23574
                     /locus_tag="PLJ75_02515"
     CDS             22171..23574
                     /locus_tag="PLJ75_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD04119.1"
                     /translation="MKQQIKNRKTIKTRIIIYCIICLIILYKHINVLADSYANASLSD
                     CLQIALQNNISIKIAFEEVNGALSNVKITKGSNNIQVNGELRTIEILKSDAANSSFNV
                     PGKDTLIGLFIGASATYKLYDANKSNYEQLKFLEYNLAILSALKIKNNVMRDVKITYY
                     QLLLAKKKSELLEKLVKSFEIKYLNAEQLFTSGQRTMVDVSKAQVDLTNARLKYERAQ
                     NDATKIKSTLVSLMGMQSDGFDIDTAGFDINNVVKELPYNYDELLQLALLYSFDIKSA
                     EINQELSKTQINIEKDKRLPQLNVVASLGYENREIQNGSFSESLSDRGNWKPTFHAGV
                     SANFPIYTSGVVSSSIDRAMISYNKAIYNVKTVTDSITSNIKSYYTTINDIKIQLDIA
                     QQLIDNAKKYLDLVQKNYDIGIITQNELQDAELNYIQANIDYMQIITDYYVNFHYLIS
                     LLCIDENTLFSKGAALK"
     gene            23571..24668
                     /locus_tag="PLJ75_02520"
     CDS             23571..24668
                     /locus_tag="PLJ75_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPD04120.1"
                     /translation="MIKSFLTKRKTIVIIGIILIAIVIIIRPCSDKSGYEYDFGQVSI
                     GDVSRTISTTGTIDLLNPVSILSQVDGVVKKVYVDFNQRVKQHQLLAIIDAGDLNNEI
                     AKMQKTIERLKIDLQNTEKDLNVKRSMYKENMISNNALQLAENEYKKMQLSYSSVIVD
                     YNNLLQMLRDTKIYAPISGIIVSRDINESDPVTRNKRLFLMVDDLKNMSITISIDETD
                     IGKVKTGQIIKFTVNAYPDQMFHGTIKQIRFNPINRGGVITYDAIAICDNSQYLLKPG
                     MTAIVTIEVEKKQKILIVPNEALTVIPPTIDMFKLDKNKKYVWKKKGGNIVPVEIQTG
                     LLGDSYTEILKGNVKNGDKILIRVRKSVSIK"
     gene            24686..25405
                     /locus_tag="PLJ75_02525"
     CDS             24686..25405
                     /locus_tag="PLJ75_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04121.1"
                     /translation="MNAAIIGIENLHKIYKLSEEISVHALKGVNFKVKRGEFVAIMGA
                     SGSGKSTFMNIIGFLDTPTSGRYILDGIDGTSLNENQKAEIRNRKIGFVFQGFNLLSR
                     TPAIENVELPLFYRGGFSHEEMRERAGKFLAMVGLSGRETHHPNKLSGGEQQRVAIAR
                     ALVNDPAILLADEPTGNLDSKNTSEIMNIFTYLNKEYNLSIVMVTHEPDVAEYADRKV
                     IFKDGLIIDEVIIKKLKKRKK"
     gene            25407..26585
                     /locus_tag="PLJ75_02530"
     CDS             25407..26585
                     /locus_tag="PLJ75_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD04122.1"
                     /translation="MDFSNLLITALRALAKNKVRSILTSIGIVIGVSSVIVMIGIGQS
                     ASIAVEERVGTYGKNAMEIASYRNLLTENDSIYIKNNIPQVNYVSIVITKSNVQINYK
                     DRALMSALYGVENDYFKMKIWPLFSGRYFTDMEIDTHERVVIIGSTVMREIFLYEEPI
                     GKTVIINSVPFKVIGVLSELGQSFGGKDFDNIVIMPYGTVQRKIEGNRKIDRIYVSVY
                     SEQQLDETIKYIRDYLHRVHRIPPDVNEFRITTSKERLEMAEYISKTLAMLLAGIASI
                     SLFVGGVGIMNIMLVSVSERTREIGIRMAIGAKRRDILSQFLIESITLSGLGGIIGIL
                     LGLFFYYIITYIVDWPYIFSFTSVIISFLFSCAVGIFFGYYPAKKASDLKPIEALRYE
                     "
     gene            26758..27648
                     /gene="xerD"
                     /locus_tag="PLJ75_02535"
     CDS             26758..27648
                     /gene="xerD"
                     /locus_tag="PLJ75_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPD04123.1"
                     /translation="MKDIIAIKRFKRFLQTEKGLSPNSIYSYIYDLKKFSDFLANKNK
                     TILNASQEDIEQFLFFEKNKKRNSARTLARSLAAIRQFYNYMANIEHIANPTVKIETP
                     HFQKALPDFLSIEEIDRLFSSIREDDMYQLRDKTIFELLYSAGLRISEAVELKAVDVD
                     LKNKFIKVMGKGNKERIVPIGDEAIRLIEKYMRDSRPVILGSRMSEYLFISKKGGMLN
                     RKSVWRLLKGYAQMVNIRKNITPHTLRHSFATHLLERGADLRAVQELLGHVDISTTQI
                     YTHLARKQLQEIHKRFHPKG"
     gene            27690..28832
                     /locus_tag="PLJ75_02540"
     CDS             27690..28832
                     /locus_tag="PLJ75_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPD04124.1"
                     /translation="MYVIIALLVMIGCVPSPEYLAKTGKRVGLDNDYIRVLLLKDQVK
                     VIIETTSKTKVVQLSNGTILYNGNPKHLEYFIHKLPETLQFETWNNEIIVNGIKVRGM
                     IQLIPVVGKIQVVNVLKMNEYLYGVLPSEIMPGWPIETLKAQAVAARTYAYYHIMKNN
                     STYYDLDASTNFQVYRGAISETETTNKAVDSTSGIIMVYNSIPILAYFHSTCGGRTAD
                     DRFVWKGEDLPYLTSVNCSYCKNSPVYSWQVNISLDEIYEALLKKYKTVGQIKAITLG
                     REDNRVSNIKIEHSNGIIRMTGNDFRMMFETKKIKSLYFEANQTHDGLTLNGHGWGHG
                     VGMCQWGAKEMAGRGKNFYDILKYYYRGISVVSINTVPQKYRKSNF"
     gene            28899..29939
                     /gene="queA"
                     /locus_tag="PLJ75_02545"
     CDS             28899..29939
                     /gene="queA"
                     /locus_tag="PLJ75_02545"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPD04125.1"
                     /translation="MKKKIYTLSDFDYFLPEELIAQYPAEHRGMSRLLIVNRKDGQLI
                     HDSFYNISHYLHKDDILIFNNAKVIPARIYCRRSTGSKVELVLTKHDTINNTWIAICN
                     RLKRLKAGEILYPIGNENINIIITEKLDDEIIIKTNPDLSNDILAKIGQLPLPPYIQR
                     APEPMDYERYQTVYAKKEGAVATPTAGLHFTEEILKQLHNKGVAIAFLTLYVSWGTFH
                     PVRHEDISQHTMHYEEYYLPPETVDAIYNAKSKGGRVIAVGTTSLRVLESVYKEGEYI
                     SGYGETNLFIYPPYTIQSVDALVTNFHTPKSTLLMLVSAFAGYDCIMNAYDEAIKHHY
                     RFFSYGDAMLII"
     gene            30272..30802
                     /locus_tag="PLJ75_02550"
     CDS             30272..30802
                     /locus_tag="PLJ75_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD04126.1"
                     /translation="MHTHTTCSDGTLTPQQVALIYEEKGYDFIALTDHDYLLKPGFEK
                     IYNNVKTSLMLFSGIELTVFLKGYIHINRIFGDVNLLHIFNHLPEYDLDIGQIMNRLY
                     YLQKMFQIDAVEITAKGFKVDGFDTIHLPYHSIVSDDAHTEVGIGRAWIELDAKKNKD
                     SILRTIKHGDFWNCYC"
     gene            30854..31999
                     /locus_tag="PLJ75_02555"
     CDS             30854..31999
                     /locus_tag="PLJ75_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD04127.1"
                     /translation="MANRVAICAVAQIKNDPNLWHARFQNMLLECFEGIISQTNVGFD
                     MEKGIRTVLTCSDDVWDARTISDNAITDVVGAHFRGEEKIAQESLNGLGYAMACILSG
                     HDDIILYMGHMKESQTESRGMCTNLAFDPFYCRPLGMDFVNVNAMQARAYMATSGINE
                     EHLANIVVRSRKNAKKNPYARNNVLVKEDDVMKSPLLSDPLRELHYYPATDWAFGMLL
                     CNEKRAKEFTDKPVWMTGFANCIDSYFLGDKDLTSNFTLKNATERALKKAGIKGLKNN
                     FELYELCDHAAYQLPMWAEGIGLIKDGDGGKWISEGGLDECNINISGGMLNGNPLLLG
                     GAARVIECFYQLRGEADKRQVKGVKRALAQSTYGAAGQHQAVVIMEV"
     gene            32013..33209
                     /locus_tag="PLJ75_02560"
     CDS             32013..33209
                     /locus_tag="PLJ75_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD04128.1"
                     /translation="MAIEKNRNVAIIGIGQTKHSSHREDVNQPEMIYEAVSSALKDAN
                     LSIKDVDCVVHGNMELFEMIHQPDLWHVLGTGAYGKDSIRITTGGTVGATIACVSDNL
                     IASGMYDVVMAIGFEKLQEGHTTGGITNMADPLWFRNLQTGALTGSTAYDVMQEFGED
                     RAKRASMMYRIIMDKHACLNPNAHRAFGLEFDQIETLMNTSPQLVGELRLIHMCSQSD
                     GACAVILASEKKAKELSKKPVWIRDHITIHREETFNIFGYHQKYPVYKTHKFAAEQLF
                     KRNDIKQPLEYFDVFEMYDPASWWGLDWLRDFFFLKGDEHLKLVENKEIMIGGSMPIN
                     PSGGVIATNPIGATALLRVAEAALQIRGDAGAHQIPNNVKHALASGFGGTMWTVLMLL
                     EKELNW"
     gene            33219..34400
                     /locus_tag="PLJ75_02565"
     CDS             33219..34400
                     /locus_tag="PLJ75_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPD04129.1"
                     /translation="MEYWGVKVEDIFNTMHNRFRPEGAKGVVAEFGYDIIGEGKWKIK
                     VNDGELEVIKTDDISNCVAVMVCDGETFVGINIGKVDATNAFTSGKVKVEGDFGQFGK
                     TAKMFKKFVLKKAAMSVRDYILDMFGTLVQRFQPNNAPQEAIIVYDIGGEDGGVWTAY
                     IKDGKCELKEESPAKFSVKLEVQEAKDWVDVMLGKTDAFSLLSAGKGSIVGETQIALK
                     LEEIFSKYQDPMAGGTPEQELVVLKKIISVDQKFSTGPVMGKFLNALKNKKILGNKCP
                     KCGRIQLPAREVCAECRVRVDEWVEVGPKGQVRYMEYVYYASPDPLTGETRETPYGMA
                     NILLDGCKGNDTFAHLIRRDQIDLIKGGKDDVSGTRVRPVWNEHRTGSIFDIKYFEID
                     E"
     gene            34413..35840
                     /locus_tag="PLJ75_02570"
     CDS             34413..35840
                     /locus_tag="PLJ75_02570"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPD04130.1"
                     /translation="MALVYDKKDCFIVEGKLALPYSYFAGRVGSTFITTLRDKKKIMG
                     VKCPVCNKVYLPPRQTCERDLTDIRDNWVEVQPAGEVVNFTVVRYDDKYLPRKAPFVM
                     ALIKLDGADTPIVHILDGVSPEEVVIGMKVEAVFSSSPTNTILDIAHFAPKKPEKVYV
                     SQRKTGAKEKEGAQPISDEEKLKQERRKGMSKKVIITAALSGAATMKNQNQAVPYTPQ
                     EFAEESYKCYKAGAAMIHVHAREDNGMPTHDHKRIKDTHDAIKEKCPELIVNLSSAVG
                     MGKTPEQRISQILYVKPEMASLNTNTMNFSIVDRKTGKVFIDFIFENTFTMLQDFGKA
                     MEENNIKPEVEVYDLGGLDNWFLIAKQGFFTKPYNFNFVWGVAGGMEFRPDMFMVLKN
                     ALPEGANFTTCGVGTEQFPSINLSCMVGGHMRVGLEDNIRIPTGELAKGSYEQVEWAV
                     KIAQILGREPATPDEAREIMGLKKQ"
     gene            36385..37164
                     /locus_tag="PLJ75_02575"
     CDS             36385..37164
                     /locus_tag="PLJ75_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPD04131.1"
                     /translation="MNDKCYYQIEKENVAVVYFNNPPINALDTETMESFEEIMRILIT
                     DDKVHVIVLTGKGSTFIVGADVNEVAKVNTFDKGVEITTKAQAIVSLCELSPKPVIAM
                     INGLCLGGGMEIALSCHIRIASNTAQMGLPEIMLGIIPAFGGTQRSPRLLGKAKALEF
                     MLTGMFINMQEALQIGLVNRVYAPDELIEESLKFASSIAKKGQIAVRSIVEAVMKGSS
                     MNLEDGLRLESTLFGRLAESHDKKEGIDAFLNKRKPIFKHC"
     gene            37182..39008
                     /locus_tag="PLJ75_02580"
     CDS             37182..39008
                     /locus_tag="PLJ75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD04132.1"
                     /translation="MEEKTINQVFRNRVQKYGSRIALEKKLNGKWESVSWEDYYKRAA
                     YVGLALNDMGLQKGDRVSLLSENRLEWLYADMGVLGAGGCIVPIYPTLIDDEVEYIVS
                     HSDSKFLIVENNAQLQKGLYTAKRYPGLKKIIVIDTTEVNVNPMIMDYNELLDKGKVL
                     YNKNPEAFASLADTIAQDDLATIVYTSGTTGVPKGAMITHKNIMAVIKALDTIEPHYA
                     YDSDQTVPFLPLSHVFERVAGHFYGMYVGITASYAESLDTLIQDIQEKRPTVVLAVPR
                     VLEKVYQQIISQVQEQSPFKQKVFYWGQKVGSKISELREQKKNPGFNLSLKYKIAYAI
                     IFKKLQNALGGRVRWMTASGAPTAREIILFFNSAGIMVIEGYGMTECCAPATMSNIAD
                     YRIGTVGRPLPGVDIKIADDGEILIKGDNVFKGYWKMPEETKDSFTQDGYFMSGDIGL
                     FDDAGFLMITDRKKDLIITSGGKNVAPQKIENLIKSDPLFTEAIVIGDKRKYLIALVN
                     ISQEQAERIAKEKGINVNSLHDLFNNPRFQTIVEEHIEKCNQKLARYETVKKVGIIKN
                     EFSKETGELTATLKVKRKAVQQKYQPIIDSLYEEDAKTIQIY"
     gene            39086..>40098
                     /locus_tag="PLJ75_02585"
     CDS             39086..>40098
                     /locus_tag="PLJ75_02585"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPD04133.1"
                     /translation="MALGTRDNDTEGVMLSGKNELFTDPDPDKARAFFRNKSRSMTPK
                     LAQIKDVVRDMIKDGDYIAIGGFGANRIPTAIVHEIVRQHKKHLGFAGHTSTHDFQVL
                     IAGKCIDRCDVAYIIGLEARGLSPNARRVVESGTIKLTEWTNASLSWRLKAAAMGISF
                     IPTRNILGTDTFKHSAAKTITCPFTGKKYAAMPALFPDFAAIHVHEADIYGNCRVYGA
                     SVSDFDLAKASKRVVITTERIIPHDEIRREPEKTVIPYWCVDAIIEVPYGSYPGNMPY
                     EYFSDEEHLKTWLKVERDPEELEIFLQEQIYGVNDFYEYLNLNGGIEKMKKLRAIEYL
                     INN"
     assembly_gap    40099..40198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40199..40564
                     /locus_tag="PLJ75_02590"
     CDS             <40199..40564
                     /locus_tag="PLJ75_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HPD04134.1"
                     /translation="SLCWRTMMMTVQDARRFTDKLDFITSPGYLKGGRSRYDAGLPEG
                     TGPYRIITNMAVMGFDDETKWMMVLSINPGYSRQDVQDNCGFELKWAKKIDDTKPPAD
                     EELRILREEVDPHHYIIGR"
     gene            40577..41038
                     /locus_tag="PLJ75_02595"
     CDS             40577..41038
                     /locus_tag="PLJ75_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPD04135.1"
                     /translation="MGNIFHGIEPSVYKSQINVPYQWWAGETASKYFIALRDEKKILG
                     KQCPQCGKVYCPPKKTCPSCFTETNAWKEVGPKGKLISYTIVLRQLAALQKKVPVAFG
                     LICIDGADNAMLHYIGDVDVNNITIGMEVEAVFADDRNATINAISHFKPLT"
     gene            41049..42188
                     /locus_tag="PLJ75_02600"
     CDS             41049..42188
                     /locus_tag="PLJ75_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD04136.1"
                     /translation="MDAAIIGIGMTTIEKNKIACNYADLAWEAVNKALADASITIDDI
                     DNVITTSSDFWDGRTISCMAVGDACAVRHKNCSAVEGDGTFGAAYGLSRTLSGDYKTT
                     LVTAHSKGSESISSIITNAAFDPIYERSLGLDMISASAIQAKQYMHVTGTTPEQLALV
                     SCKNHKNGKDNPYAQLPMDITVNDVLNSEMIAEPLHKLDCSPVSDGCCAIIIANAEFV
                     KKVKAKPVWIKGVAFCSDAYFMGDRDLTKTIALENAAKKAYMMAGITNPENEIDIAEI
                     YDAFTYQELMWIEGLGLAEYGNAGKLLEKGIFNKNGKLPVNPSGGLLSGHPVIAAGLY
                     RIAEIVLQIRGEAGARQVNAKRGIAHGVNGICGQSHCVWVLDKEK"
     gene            42198..43361
                     /locus_tag="PLJ75_02605"
     CDS             42198..43361
                     /locus_tag="PLJ75_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD04137.1"
                     /translation="MAKRRVAIVGTGQTNHKSHRPDVNGQELIHEAVVRALQDAELSI
                     NDIDAIIIGNMDHFEGINYVDCWSIDGSGGYHKPIMKLTTGGTTGSTLAIGGYHLVAS
                     GMFDKVLVIGWEKNSESDTTGAITTAFDPIWDRLVFAGAISGLAVEAQSYMAKYGATD
                     KDGARVSVRDRKHACNNPYAQLRKEVTLEEVLASPMLADPIHLLDVCPRTDGACAVVM
                     ASEDCAETITSTPDWIWATANRHTYSYLGDVNWGELDSIADASKELFKKSGISEPRKQ
                     IDVIEMYQPYSLAGIIWIEDMGIIPRGKGAEYIWSGATDMGGELPINPSGGVIACNPI
                     GATGLIRCAEAAMQVMGKAEGRQVPDVNFAVSTGFGGCMWTDMILHGKNKPNL"
     gene            43372..43836
                     /locus_tag="PLJ75_02610"
     CDS             43372..43836
                     /locus_tag="PLJ75_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HPD04138.1"
                     /translation="MENLEHIIIESGKAVQPFHYSVGMYGSKFFHELKNNRKFVGVRC
                     PKCKKVYVPPRQVCGECFMEMKDFIEVGPKGIISTFTILRFAFIDPETGLQKPVPYGY
                     GFIKLDGADTLFQHFIDVTEESKVKIGARVEPVFSDEKKGTIKDILYFRIID"
     gene            43840..44631
                     /locus_tag="PLJ75_02615"
     CDS             43840..44631
                     /locus_tag="PLJ75_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04139.1"
                     /translation="MSLIEVNNDMRVIMDREAFFVMLLAGTDKIRSGYGNGDSLLIDS
                     EHSIFAIADATERFPNASRILLSKIMGGLASIPDTSEQWRAYINAIYRQQEYHLKTTL
                     SLIALKQSHNGCKAFVVHGGDSSLKIFNRLNKKLLYKSTANMNFAGRSTSIDTIDSIN
                     LESNTRIIIHSDGFNDIMKIGAIDFDDIIAAPLFDCISLILSQVNAKNFNNEYDDVGL
                     LIVDVPEIFNVISGGILMGGTTHCEERCLQNNLTICDYSSLIKTF"
     gene            44686..45312
                     /locus_tag="PLJ75_02620"
     CDS             44686..45312
                     /locus_tag="PLJ75_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04140.1"
                     /translation="MRVKQERNSIYDTRRLQLTAAAYNVVSKKGYYNFTIKDIADEAG
                     MSAGLVHYYFKNKQDLLLNLLRQINTNIRLALQNDLANIEDPVDKLSVFILRACNLII
                     EEKNYFYVLLDFWTQLNHNERMRKAIQKLYQSYRKVCSDILNEGIIKGKIKQIDVIYT
                     ATMIVSMVQGLIIQYVIEPEAFDYSYYVNKIKNQIIETIISKGVNNGI"
     gene            45302..46450
                     /locus_tag="PLJ75_02625"
     CDS             45302..46450
                     /locus_tag="PLJ75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013260040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD04141.1"
                     /translation="MEFSFSEEQLLFRDTIYKYAKNEIVPLCEEADLKSEFSFEIWQK
                     LARMGLLGLPFPEEYNGQGADVVTCCLASEALGHAGVDSGHLLALGAHTYLCTDTLFK
                     HGNEHQKKTYVHKLANGEWIGCMGLTEPGAGSDAAALQTTAVKKGDRWILNGSKTFIT
                     NAPVADVCVVYATVDKSKKHAGITAFIVEKNFKGFSTGKPFHKLGVRASTTSEIFLED
                     CEIPEDNVLGNIGEGFMYAHQTLEWDRSALLAPFVGAMQFMIEQCARYATERVQFDKP
                     IKSFQAIQHKLADMKMVMEAARLAVYRVASLKDAGYPINHLQASIAKAFVGDWGFKVA
                     SDAVQIFGGYGYIHDYPVERMIRDAKLAQIGGGTSEIQRLIISRLLTL"
     gene            46476..47645
                     /locus_tag="PLJ75_02630"
     CDS             46476..47645
                     /locus_tag="PLJ75_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD04142.1"
                     /translation="MNYELNDIQKSIKENFKKFCENEIKPNASILDAGDEKAVELLKN
                     NIKKLGQLGYMGMIHEEAFGGTDQDLINFAIAGEEVSKACAATFLSAGASCGLCGMPI
                     RLFGNDKQKEKYLPGIISGDVIGSFGLTEPQAGSDATAIATTAKRKGAKWILNGTKTF
                     ITNAPIADVCLIMAYNDKEAGPGAGVTAFLVDTHTPGFSIGKPFDKLGYRGSPTSEIF
                     LEDCEVSDDAVLGELGKGFIQAMQTLEYGRIGMATVSLGVAVACMDEAVKYASVRKAF
                     GKPINRYQEISFKLADMMILTDLSRLLIYKAAWAKQMNDPEAAVLASCAKLFASEAAT
                     QIASWALQVHGGYGYIKEFPVERLYRDAKLGEIGEGTSEIQRILIARDILQKYGS"
     gene            47660..48097
                     /locus_tag="PLJ75_02635"
     CDS             47660..48097
                     /locus_tag="PLJ75_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPD04143.1"
                     /translation="MTVDKKFIGKTWPTVIYEVGKEKIKEFAQAIKSNNPYYFNDEFA
                     LQSKYKTIVAPPTFAVVFGAKLIEPIFMDNELSLNIAMLVHGEQEFEFYTMVKAGDIL
                     YSNARIIGIENKEKLDVVTIEIMTKNQYNQDVCKGIYTFVVRK"
     gene            48109..48510
                     /locus_tag="PLJ75_02640"
     CDS             48109..48510
                     /locus_tag="PLJ75_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HPD04144.1"
                     /translation="MYEKNNIDAGDSFKVQQEIDKYRAIYYAGASGDFNPIHIDAEFG
                     KMVGLGGAILQGLCTLGFTADAVTTWIGDPGRLKKLKCRFSLPVKMGDIITIEGLVTE
                     VRGDSGTVEIKVINQNGSDVLTNVQAEVVAK"
     gene            complement(48623..>50483)
                     /locus_tag="PLJ75_02645"
     CDS             complement(48623..>50483)
                     /locus_tag="PLJ75_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPD04145.1"
                     /translation="SFLTAALTFIIAFLQLCKSIVMYPQLYLDTIFNKNIIYKNFQIL
                     LTNNFSPDFFMALQCSIVVPFIAAFVFVVYRQLFNHMKIYFQQMASLRNKKKQMIVAC
                     VIVTIVTISLLPITIAHNKTTTKPNIIILASDALRPDHFSGYGYYRKTTPTIDTLIQQ
                     GANFHNVYTVVPRTFPAWVSIITSQLPMKHHISHMFPMAVTRKQNFITLPKILSHHGY
                     YTSVVGDFAADIFPRIDLGYETVKAPTFNMRVMIQQIILKNHTFLLPFLTNKAGFYCF
                     PEIKEFAEFADPEFVVQDIKTEISKSIKTKKPFFITSFFSITHFPYPACYPYYNLFTD
                     RHYSGPFKYSKNRVVSLDSEGEAHEYSPSDNEVAYIRALYDGCVYAFDEAVGKVIQYC
                     KDKNIYDNTIIIILSDHGENLYEYDYGMGHGEHLRGKYSMVIPCIFAGPGIPYQHHRA
                     VRSAIDIAPTILDLLGIQKPESFDGTSLLEKHKRNFDAYCETGIWFDNTGNFFFQKKR
                     IMYPDITGISTIDFHYHDEITITPYYEYCITIAKHRCIIANNYKLIYMPTHYGIEYEL
                     YDLSNDSDERNNIFTTSLQSEMLKKKFFSLFKDDKTFTLYNEYLLPVIDKPIF"
     assembly_gap    50484..50583
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            50834..51598
                     /locus_tag="PLJ75_02650"
     CDS             50834..51598
                     /locus_tag="PLJ75_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPD04146.1"
                     /translation="MDTNVLLKKNNYVATIILNRPPANSINIHVREELYAIVKELEDD
                     NTIRVLIITGAGDKGFSAGMDVTDVANLDKGPDAIELFNYIDRYPKPVIAAINGYALG
                     GGCELALACHFRLMTDMPKAFIGCPEVNLGITPGWGGIQRMTRLLGRSKALDCILFSK
                     RLTPQEALSIGLVDRVFPHEHFMEEVQNFADSLARRAPLAVSCILKGMITGIEKGIDE
                     GLKVDRQCSAMLAKTEDAAEGFMAFAQKREPVFKGR"
     gene            complement(51645..53450)
                     /locus_tag="PLJ75_02655"
     CDS             complement(51645..53450)
                     /locus_tag="PLJ75_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD04147.1"
                     /translation="MGTKFYSEENLKFLLQEVHDIDEIFSCDTFSDYNVESMNIILDT
                     AKNIASGMMYPYFSEMDKNPPEYTGSTIKVHPSVKPFLEKFAEGGWINASFPSDIGGQ
                     QMPFILFNACMFIFASANYSMSVYPGLTSGALALIYQFATKELYQIFAPPMIAGKWQG
                     TMALTEPEAGSSLSDVKTMAIPTDKGYYLIKGQKTFISAGDHDAVENIVHLMLARIKD
                     APQGVKGLSLFIVPKYRPDENGKLIFNDVNCAGCYHKMGYKGAPIAQLSMGEADDCRG
                     YLVGEPNKGLSYMFHMMNEARINVGLGAAAIAGAAYQTSLEYAKERRQGRKITEKNPS
                     VPQIPIIHHADVKRMLLKQKAIVEGSLSLIIQTGKLVDLINTSAGEEKEKYELFLDML
                     TPIVKTYPAEMGVVSTSLAIQCMGGYGYSSEFPVEQYMRDSRIHPIHEGTTGIQGMDL
                     LGRKVTMKNGATAKVYMTEIYKTIASAKQYPSLISYAEDLDKAAALWQEVTGFLIQFA
                     VKGDIERFLADATLFLEFSGLICIAWQWLLQGIAVQHSINNSSGSYSKDFLEGKMQTL
                     AYFFRYELTGIYALEKIIRGSDGLTVSMQENYFED"
     gene            53763..>54131
                     /locus_tag="PLJ75_02660"
     CDS             53763..>54131
                     /locus_tag="PLJ75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD04148.1"
                     /translation="MEEKSVMQDQRTITFTEFITKFIPFTIRIPYILYHGIKGLNMIS
                     GKKNISWGLLLEKTAEKYPNNIAIKSHEGTLTYKELNEKANKFAHFLKSKNIVKGDTV
                     TLCIDTRPELLVLYCGCTKIG"
BASE COUNT        18216 a         7977 c        11170 g        16468 t
ORIGIN      
        1 gaaaactatc agatgggtgt tgagtggttt gttattgatg ggttggcaat aggtggcgga
       61 atatcctata atacatggga tgaggcagaa acagaagtat tcaccgtaat gccaacagta
      121 acatattact atgcaatgga taagcttatc ccctacatag gtgctggatt caaatatatt
      181 gatatttcag atgatccagc gcttggtgat ggcgatggat attctatttt tggtaaactg
      241 ggtgtggcgt atatgctggg gaacaacctt gcattgtttg ctgagttcca gtatagctat
      301 gatacacttg atttggatgc tggtgatgaa gacggaaacg tcatgagtgg atctcttggt
      361 gttaaagctt tcttctaagg attagctatt gatactattg aaataagttg aattgacaca
      421 tttccagggg caaaatactt ggtaacaaaa ttatgaggaa gagcgctgta taaaggcgct
      481 cttttttttg tcagcagcag tgtgtaatga taaaattact tgacatctat acataagtgt
      541 agataaaact attcccatca ctctgaaaga ggtatatatg ccagcatttg tccatatctc
      601 ggatacccat cttggattta atgatcttga cagggttgat gacaatggca tcaacgtccg
      661 cgaggctgat gtatatggtg cctttgaaca tgctgttgat cgcattgttg ccatgaagcc
      721 tgattttgta ctgcacaccg gtgacctgtt ccaccgtgca tcgccttcca accgtgcact
      781 gattcagggt ttaaaccata taaaaagaat aacacaggcc gggattccgt ttattgtcat
      841 tgcgggcaat cactgtacgc caaggactat ctatacaagc cccatattgc aggctttgca
      901 aactattgaa ggcgtttttg cggtgtacag tcagcgctat gaatatgtta ctatagatgg
      961 tatccatata cattgccttc cccatattca tgatgatatg cgctacgttg aacagataag
     1021 gacaattgaa ccaaaacagg ggtgcaacat tctattgatg catacgtcgg tgggacagga
     1081 ttatctgctg gatgagtacg gtgaacgtat tttccccgct gagtattatc atcttttcaa
     1141 tgcatttgat tatgttgcac tgggtcactg gcataatttt aaggttttgc ctccatggga
     1201 tactgtgtgc tacagcggtt caactgaacg tttcagcgac agggaagctg gcagggaaaa
     1261 aggtttttta acagtaacta tcgcacagaa taaaaatatt acgtttaacg ccataccaat
     1321 acgtccatgg gtgcgatttg atattacccg ttgcagcgag cttccaattg atgacattaa
     1381 aacacgcata caacattttt catcacaggt tgaggatggt tcaatagtca gcatttatct
     1441 tcatgatgtg gacacgcgcc agagtgccgg catcagcagt gtgtggatta ccgagcagtt
     1501 taaaaacgct atggcggtaa ttccaaaacg aatttttaaa aaacagggtg gcgatagtta
     1561 ctttcaaagc gagcatgttc cggtggatga gctttttgtt cagtattgca ccaacgctat
     1621 agccgatgaa aaggaaaggg ctttgcttgt tgcatcgggg agacgatact tcaaaaatta
     1681 ctatgacagg gttgatgaaa aaagggaata gcctatgatt atccagtcgt taacgcttgt
     1741 caattttaga cagtatcgtg atgaaacaat agaatttaag gaagggctaa ttgggattat
     1801 tggcaaaaat ggcagcggga agagcacgct gtttaacgct cttgtgtgtg ctctctatgg
     1861 tgagctaccc tcaatgaagg agcatatacg aagcagtttt gtacagccca atgaaacggt
     1921 gagcgttacg gtatgctgtg agattgaaaa tagcgactac aaaattatcc gtgagttcaa
     1981 agggaaaaat ttaaccccat atgcggcatt gtataaaaat ggcaacgaga tagccgttgg
     2041 agcaaccgat gttacccgca ctattgaaag aattataggc atgccaaaag aagcttttac
     2101 ccgttcggtg tttgctgctc agggtgaact cagagaaata tcagaatcca gaggcggtca
     2161 gcgccgggag cttatccgca agatgatggg atttcagatg ctggatgata tattgcaaac
     2221 tattcgtgac gataaaaaca aaatgaagaa tgtcatcgat ggacagcagg nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn caaactattc gtgacgataa aaacaaaatg
     2401 aagaatgtca tcgatggaca gcaggcaatg ctgttgcttg atagcgaacg agatgaaaaa
     2461 aaagctgcgc tgaagagttt atatgatgag ctgtcgaaca taaaaaaaga atacgatgat
     2521 gtaacacgta cacacgacaa catgctacaa cagtttagca atgtacaaaa agagtttgaa
     2581 aagcagcaaa aaatttttaa agaatatacg gtgctacagc aacagttgac gcaggaaaaa
     2641 accagattgg aaaattgtat aaaaaacatc agcgaaacag aagaggcgct gaaacagttg
     2701 tacaaagcca gcgatgagtt ccatgcattg catgagcagg aggtaaagtt taataccgtt
     2761 cggaagcaaa aagatgagct tgacaccgtg cgggttcgtg atgctcagaa aaagcaactg
     2821 catcagcagt ttgcgttgat tgaaaaacag atacacgaaa aaagtgaact gttgcaggct
     2881 gaaagcaatg aagtgctgga atatgaaacc atactacaac agttaaacaa tgcgctaaag
     2941 gataaatcaa aaattgcaca ggcactacag gctttccata acaaaaaaaa tgatatttca
     3001 tcaacaattg gcgggataca aaaaactatt aaggacagac aaaaacatat tattcagatt
     3061 gaaaagctgg gcagggaggc tgaatgtcct gtttgtttgc gtccgttgca tgacgcctat
     3121 gatgcaacca tacagcgttt aacaaacgag atagaggagt atgaacgacg ggagatggct
     3181 tcattacaac atgctttgca aacggttacc agcaacatcg ctgaatctga aaaacaattg
     3241 agcaaaactg atgaattgat tggcaccttg cagcagaaga aagcaaagct tgaggaaaaa
     3301 caaaacacta taaaaaaaat aaaaaaagag attgatggac agcaacagtc acgggaagat
     3361 atcaggatga agctggatga tctttcttca gtttcgtttg atgaggcagt ttataatgat
     3421 attgtgcaac agtatcataa actggaagca atgcatacac gataccttgc attgcaggaa
     3481 aagataaatg gtattccaga atctgaacaa aaaaaagctg ccctcaataa agaaaaggaa
     3541 gcactgcatg ctgtaataca tgatgtaacc aaacatattg aagcattgca ctattcggaa
     3601 catgcttacg aagcggtaaa aaatgaattt gatgcaatgg caaagactaa agatagcatt
     3661 gtacaacgcc tcaatgatgt tcatttgcag ttaaccaatc ataataataa aattacacaa
     3721 attgaacatg agttgcaaaa agatgatgaa aaccgccaca aggttgaaaa agccaggaat
     3781 gagctgaata ttgtactgca gcttgagcaa ctgatggatg gatttagaac acaagtttta
     3841 cagtttataa agccagctat tgcacagtat gccgggaacc tttttaacca gcttacacag
     3901 gggcggtatc aatcaattga tatggatgaa gattttaatt tttttatcat ggatgatgat
     3961 gttcaatatc cattaagcag attttcgggc ggtgagatag attgcgccaa tctttgtctg
     4021 cggattgcaa ttagcaacgc tatcagagat ttcagtggag gcggagccgt tggttttatg
     4081 gcgtttgatg aaatctttgg cagtcaggat aatgatagac gtgcagccat tatggatgtg
     4141 ctatacaggc tgcaggagca ctatcgtcag atatttatta tctcgcatat agatgatgtc
     4201 aaagagttgt tcccttccat actgcacatt caaaatagtg gtactggttc aacagcaaca
     4261 tggcttgcgt agaatgttag caatgtatgc actattgttt ttacaaatat aatgaaaaat
     4321 tttggcgata gttatcctga atcatgaaat cggtaatgag ggaagtaaaa gcaaagaatt
     4381 atattgtgcc gtgtcatcct gaacaaagca attgtcattc tgaacgaagt gaagaatctt
     4441 gtcttttgtg aaaacggaga gattcatcgg ggttgagtcc ctcagaatga caatgctgga
     4501 atatgtcatg ctgagatggc agttgcatgg aacatgtaaa gagatactga tagcattgga
     4561 caattataaa aatggtgaaa atcaatatag aatgacgcaa aatgcttgct attattataa
     4621 ttatgagcaa tactatcaat tggtaagatt taacagtacc aaaaaatggt atggaggcga
     4681 cccggagcga atagttacag catgaaaaat aaagcttgta ttggatggta gtcatcataa
     4741 cgtttcaatg gtaaatgcga ataaggaaaa tgtgctctgt aacttttgat ggtaaaaata
     4801 gtatgttgtg tgaaacagta taataatact ggagaggatt atatggtaac acggaaatca
     4861 ttcttaaaca atataaaaga tttattgatg gctattcctg ttgcaggatt accatggctg
     4921 aaaaatattt acgcaaaaga tatggtaagc agcagggatt tagtagttgt aaaagctaaa
     4981 actataactg atgcatccat tgcagcgatg gttaaagtag gtttacagga aatgggtggg
     5041 attgaacggt ttgtaaaaag gggtagcaaa gtaatcatca aacctaatat agcttttaat
     5101 tccacacctg acagagcaca caccaccaat ccttccctgg tggagactgt agcacgaata
     5161 tgcaaaaatg ctggagcgca ggtaacgata atggaccgcc cggttcataa tgccagatta
     5221 tgttaccact caagcaggat tgaagaagca gcaaaaagag caggtgttaa ctgtgagtat
     5281 atgaacaggg acaggtttaa aacagtacat gtgccccagg ggcttaattt agataccgtt
     5341 gatgtttatg aggaaatact ggaagctgat tgcattatca atatgccaat agccaaacat
     5401 cacaatgcag ccatggtatc actttcaatg aaaaatctga tgggcgtcat tggaggtaac
     5461 cggggttttt atcatgttaa ccttcataga aatattgttg atttcaataa ggctgttaaa
     5521 gttcatctta tcatcatgga tggattgcgg attttaacaa aacatggacc atcatcggga
     5581 ggtcccgaag atataaaaga aacaaaaaca attatatttg gaatcaatcc tgttatggtg
     5641 gatacatata ctgcccacta tctttttggg atagagcctt catctattgg ttatttaagt
     5701 ctggcttcaa atgccgggat gggaagcatt aatgttaaca catattctgt tataacaaaa
     5761 accgtatgaa atcaaagcac tggtcattat tcagagctat aattcaattg gctttttggc
     5821 ttatttttgt tggcagcatt gtttatctgc atgatccctt atctgatgtt agatatggat
     5881 tgtatccttc actatccata tattcaattt tcattggctc tattacaggt catacgctga
     5941 aatatgcatt tctgggaatt gctattgcgt tgctgacagt attgtttgga agatttttct
     6001 gcggatggat ttgtccttta ggcgctacca ttgatgcttc tgatacagtg atagagacaa
     6061 aagcaaaaaa taaacagctt aaaaaaataa cgcgaattaa gattctgata cttttagcaa
     6121 cactgctgat agcatttctg ggatttaatg caacaggtta tagtgatcca ataactatcg
     6181 catatagagg atatgaatta tttctttatc catttatttc atatttgggg tcagagccat
     6241 tgtcgtgggt tttgccagga tatgctgcac gcgattaccc gttttattac tataactgga
     6301 tatggccagc gattattttt attgggattt tagcattgtc attgtttttt aagcgattct
     6361 ggtgcagggt gttatgtccg ttaggtgcta tctatgcgct gctatcacat ttcagtttat
     6421 tacgacgaac ggtagatgct gataaatgtg ttcattgtgg tgcatgtgta aaaggttgcc
     6481 gcatgggagc actatccagc gatggtacgg gaacgataga acatgaatgt atcaaatgtt
     6541 ttgattgttt aaagcattgc aactatggtg caatacagtt caggtttctt ccgccttata
     6601 aatcaaaaag cattgctcat ccggcacagg ggataacacg aaaagatttg cttgttgttg
     6661 ctggttcatc gttaatgatt gcttcaacac gcttaacact atttgctgca tcgcatgaaa
     6721 gttatattat caggcccccc ggtgcacttc cagaatctga cttttgcaat gcatgcatac
     6781 gctgcggcca gtgtatgaat gtgtgtccta ccaacgcatt acacccatgc atgattgaag
     6841 cagggtttta tggaatgttt acgccccgac ttgtttcgcg gattggatac tgtgattttt
     6901 cctgtacacg gtgtggcact atatgtccaa caggtgctat acagaaacta tcgcttgcaa
     6961 aaaaacaaac atttgttata ggcactgctt atattataaa agatttgtgc ataccatggt
     7021 cagaaagcac aaattgtatt gtttgtgaag aggtttgccc tgttcaaaac aaagcaataa
     7081 cgcttgacag gaaggttatt accaataaaa aaggggaggc agttgaagtg ctattgcctg
     7141 ttgtacatac agagctgtgt atagggtgtg gcatttgtga aaaccgctgc cctgtttcag
     7201 gaggcggtgc tataatggtg cgaaaaccaa aactgactat gggagatagt tatggctaat
     7261 gaaaaacatc atgaagcata tgtagcccgt aaagtgacat ggattggaat gatagtaaat
     7321 atcatgcttt ctgtttttaa acttgttgcc ggtattattg gtcatagcca ggccatggtt
     7381 gccgatgggg ttcacagtat ttctgatctt actaccgatg ctgctgtact tataggaata
     7441 cgttactggt caacacctgc ggatgaagat catccttacg ggcatgaacg gattgaaagc
     7501 cttgtgactg cgtttgtggg tgttgcgctt tttattgttg gtattggtat tgcatataat
     7561 ggcattgtta cagcatttga acctgatata aaaagtccgg agttgattgc acttattgca
     7621 gcgtttgttt ctattgtatc aaaagagttg ctataccgat ggacggtaaa gctgggcaag
     7681 gagattgatt catcgtcagt ggtggcaaac gcatggcacc atcgctcgga tgctttaagt
     7741 tctataccgg cacttttagc agtaggactg gcaatgattc acccaaaatt ggctttcttt
     7801 gatcatcttg gcgcagttgt ggtggcaatc tttatcatca aggtatccat agatatcatg
     7861 aaacctgtta tcatggaact atctgataaa ggtgcaccca aacctcagat atcacagatt
     7921 gattcaattg ttatgggtgt agaaggtgta aaggaggtac acagtatacg ttcgcggcga
     7981 tgcggcagcg gttattttgt tgatctgcat gtgcttgtgg atggcaatat gagtgttcag
     8041 agtggacatg acattgcacg tactgtaaaa tatcaactgc tggataaggg cccaaaggtt
     8101 ctggatgtta tggttcatct tgaaccgtat caaacctcat caaaaaagaa tagtacgtga
     8161 aggaatactc agtaacaatc agattttatg aagagctgaa tgattttttg ccggctaata
     8221 aacgcaaggt tgctttcacg gtaagctgga aaggcaagcg cagtgtaaag gatcttgttg
     8281 aatcactggg tgtaccccat gttgagattg atcttattgt agtaaacggc gtttcagtag
     8341 gatttgacta catagtacaa aatgatgatt atatcagcgt ataccctgtc tttgagcagt
     8401 ttaatataaa ccatattaca caattacgcc ctcatcctct gcgtcatccc acatttattt
     8461 gtgatgtgca tctgcgaaag ctggtaaaat atctcaggtt atttggcttt gatgtcctct
     8521 ataatgagca gtgggatgat aaagaccttg cacagattgc tgcagagcaa aaaaggattt
     8581 tgttaactcg tgataggcag cttttaatgc gcaaaattgt ggatagaggt cttattgtgc
     8641 gtaatatact gcctcaaaaa cagatagttg agatcttaaa caggctggat ttgtgggata
     8701 tggttacacc attcagcagg tgtataatgt gcaataatca tgttcgacag ttactgataa
     8761 atgatcattc ctttgataat gaaaagaaaa agattcccat aggtgtattg cagtggtgta
     8821 cggtttacta taaatgtttg ggttgtggca gaatatactg ggaagggagc cactatgaaa
     8881 ggttaacaaa gaagattaaa accatacttt cagaaaaaaa taagagaagt tgattgattc
     8941 aaaaaaagcg agattcttca tctttgcctc agaatgacac atcacttccg tcattccggg
     9001 cttgacccgg aattcacatg tgtaacgtta aattctaaaa tgatcctgaa accaattcac
     9061 gacatggttc gggatgacaa atcgtgaata ttttcatcac gtatagatgg ctataagccg
     9121 gccattgtga actactctga aatagcatgg tgatggaagt ttcatttttt gttatttaag
     9181 aaaatcatat agtaatgcga tacaatagta aaggggtatt atgaaaatat tattgggcta
     9241 ttgcgtggaa aaagagcact accatgatta ctatgttacc ctgttgcctg taggtcttgt
     9301 ttcaatagct tcatatttat caaatagcgg gtatgatgtt gtactggcaa atttttcaaa
     9361 aaaaagtact gaacagatta taaaagaaat tactcatata aaaccacaac ttataggttt
     9421 ttcgctgttt acccataacc gtagtataac tctttatata gtaaataaaa taaagatatt
     9481 gtttcccaat agtgtgattg ttcttggcgg tcctcatgca acatttcttg ataaagagat
     9541 actgcaacga ttcccatcgg ttgattgcat tgtagaaggt gagggtgagg ctgccatgct
     9601 tgcaatcatc aaaaacattg agcagggtaa accaccacgt catgaaatta taaacatgca
     9661 ttctgtcaat gatgtaagca atatcccctt cccttcacaa ttcaatggaa cattatataa
     9721 tataaatgta aacgaacagt ataaatatat tattacatca cgtggatgct ataacaattg
     9781 taccttttgt gattcgcccg ccttctgggg taaaaaggtt cgtttcagag aagttgatga
     9841 tgttattgag gaattggaaa caatttatag aaaatatggc ataatctatt tttccatacg
     9901 cgatgataat tttacggtaa aaaaggaccg tgtacaggaa ctatcgtaca aaatgattcg
     9961 taaaaagctt tacatgatgt ggaattgtca ggcaagggtt gatgcagttg atgagtcaat
    10021 gcttattgcg atgaaaaagg caggacttga gcatatacag tatggtgttg agtcaggttc
    10081 tgagaaaata ttagcattat ataacaaaaa aacaactatt gataccatta tacatgctgc
    10141 aaacataaca cggaaggtgg gtatatattt atcaatttat ctgatggttg gaatgaaagg
    10201 tgaggattat aatgatataa aaaaaaccaa aaagcttttg gcttccattt taccaggtga
    10261 ttgtattgtt tcccctgtgg cattgtatcc aggaacaaaa ttatatgaag aagaaaaaaa
    10321 gaatggagct ttaagcgata gtatatggtt taataaaaac gatgcgggaa tttttttgcg
    10381 caatgaacct gttatcaaag aatggatgaa tgaattattg atggcaacct atgcattgcg
    10441 tgacaaagca tggtataaag caaaagattt tgccatgcat aaaaaaatag ccggggattg
    10501 ctgggttaca gatattctgg aaggcgatta ttattctgat aatgagttgt atcctgaagc
    10561 tgcaactgtt tatggaaatg ttataaaaaa tataccggat aacccatggg gatacatgcg
    10621 ctatggtaaa cttcatttta tcataggaga ctatgaaact gctatgaaaa gctttcttga
    10681 agtaacaaag atagtcccta attattatgg aggatggttg aaatttgctc aaagttgcat
    10741 tgcattggga ttaaatgaaa aagcaaaaca ggcaatcaat aaagcactgc aactaaatcc
    10801 atacgatgaa agaatcaatg cattaaatag tttgttgaaa aataatcgtc gaaaataaat
    10861 gtattttttt agtaaaatta caaatttttt cttcatttct tttaattttt tcctgcaaaa
    10921 gcgtattaat agatagctca atattaataa cttgaatcct tttgaggtat ctatggcgca
    10981 gaacaatatt catgataagg catataaata ccttttctcc aatccattga ttgtaaaaga
    11041 gttgctggaa tcttttgttg ccatggattg gattaaacag gttaatttca acaaagcaca
    11101 gcctattgat aaaagctatg taaatgatga ttacaaggaa tatgaggcag atatcatcta
    11161 taaattatat tttaaagata gacagttata tctatattta cttattgaat ttcaatctac
    11221 tgtggataga tttatttcat tcaggatgct gaattatgtt atggaacttt atagggaact
    11281 catctacaaa caaaagctga agaagctacc ggtggtattt cctatactta tctataatgg
    11341 tgacagaaaa tggacggccc cacagtcatt acaggaactt attgatatgc cggaaagttt
    11401 aaagcaatgt actgcatata ttccacaatt tcaatactat cctatcattg agaatgagtt
    11461 gtcacggaag acattagaat caatgatgaa tgttatatca actgtctttt tatttgaaac
    11521 aggagatacc atggtgttcc ttaaaacagt tgacagaata aaggaattat tgcatattta
    11581 taacgatgag ccactgatgc tacggacatt gttctattgg ttaatgcatt atttgcaaac
    11641 acatggaaaa attgatgatg cagcagtaat actggaaacg atagaagaac cagaggaggt
    11701 gcacagcatg ctatccaaga caatacaaaa aatgcaagag gattttcgca aagaaggatt
    11761 ccacgaaggg attcacatag gcatagataa gggaatacaa aaaggaatta atgaaggtat
    11821 atctattgaa agaaggaata tcgccatcaa tatgttgaaa aaggggattg ataaaaagac
    11881 gataatagaa atcacgaaat taaacaaaaa gcaattggcg gacatcgaaa aaggtatgca
    11941 gtagcatacc ataaaaccgt taacactgtc aatacagatt atcatcagaa aaatagatta
    12001 tgcattatta caacaatatt attgtagata aaaacatata caatgattgc aaataaaaat
    12061 caagttgcag ccattaatgc agaacagggt gcacatctgg ttatagcttc tgctggcagt
    12121 ggcaaaacct atactatcat tcagcgaagt attgcactca ttaataaaaa attgtgtaat
    12181 cctgatgaga ttcttttatt aaccttcagc cgaaaagccg cagatgagtt aaagcaacga
    12241 attgttgcag tacttggaaa tgctggtagc agggttgttg ctgctacatt ccattcattt
    12301 tgcctgcagt ttattattaa acaaactatg aatgatataa aggtgctgga tgaagatatt
    12361 tcacaaaata taatgaagga aatcatcaat aatcatatcg cagaattgta tggtattcca
    12421 tcaatggttg tttataaaat tgtatcaaaa ttacaaacta atcgcatatc acaattacat
    12481 ggcgatttat atgcaaaaat gataatgatt aaagaggaat atgccagtta taaacgtaat
    12541 cataattata ttgactttga tgatatgatt aatatttctt caaagctgtt acatgaaaat
    12601 ccattattac ggaaaaaaat acatgagcag tttaaatata taatggttga tgagtttcag
    12661 gatacatcag aaaataattt tgtactgtta aaattgcttc tgcctgaaga caaacaaaat
    12721 gtgtttatgg ttggtgatga ttggcagtca atttataaat tcagaaatgc aaagatagag
    12781 tatatcgtta atgcaaaaaa atattttaat aatcttactg ttcattcatt aaacagtaac
    12841 tatcgctcaa aaaaagagat tgttacattg tccaatcgat tgatagcaaa aaataaattc
    12901 aggtcacgac gctggattaa agcggtgagg ggaaacggag gattaatact atttcataaa
    12961 gcttcatcgt ttgctgagga agtaactata acagcatcta tcctggaaaa atatgatagc
    13021 acacatctca taggggtgct ataccggaat aactggcagg gcacttattt gcaatctcag
    13081 atacaaagtt atggcaatgt acaatttatg acaatacatt cagctaaggg gctggaattt
    13141 gatgtagttg tgctttgtgg tgttaaggac agattgttgc ctgaccccta tactgatatt
    13201 gaggaagaac gacgattgat gtatgttgcg ttaacaaggg caaaaaattg cttgcatata
    13261 ctataccatt gtaattatgg taatcaaaag ccacaatttg ttatggaatg tgaaagatgt
    13321 ttttgatttt tgttgtaaca taccatctgg tccctttcca aacatagaga tagtgctgta
    13381 ctaaacaact ggactttaag caaccgtatg gattatattc ttgaggtaaa caatctttat
    13441 aaaggttttc ctggtgttaa ggctgtggat ggagtaagct tttcaatacc ctatggtatt
    13501 tgttttggtt tacttggtcc taatggtgcg gggaaaacaa caacaattga agttatagaa
    13561 gggattcaaa aacctgatga agggataata ttatataaag gtaaaaatcg tgatcaccga
    13621 tttaagcagg aggtcgggat acaactccag gcaacagaat tacagcaata tcttactgta
    13681 aaagagatac ttttgtcttt tagaaattta tatgcgaaac gtgctgacct gaatgatttg
    13741 atagcaatgt gctatcttga tgagatatta aaccgagata acaggaaaat ttcaggtggt
    13801 caaaagcagc gtttgcttct ggcaatagct ctggcaaatg atcctgaatt attatttctg
    13861 gatgagccaa caacaggatt ggacccgcag gcacggagac atgtgtggga tatagtacaa
    13921 actgtaaaaa tgaaaggtaa gacgataata ttaacaactc attatatgga agaggctcaa
    13981 aatttatgtg atgttgttgc aattatggat cagggtaaaa ttattgctat ggattcacca
    14041 atcaatctgc taaaccaata ttgcagtggg gttacgataa cattaccttc ggtaatagat
    14101 gttgatatgt taaatagcat tgaatgcaat tggtacatgg ttaataacac aatcgaaatt
    14161 caaactgaga aacctcatga ttgcttacgc gttctcatcg ataaaaatat tgatattacc
    14221 ggcatggtgg tgcgctcaca aaatttagaa gatttattct taaaacttac gggaacacaa
    14281 ttaagggcgt aactttttat gttatataga atctggacaa tatttgtagc ccgaacaaaa
    14341 gagttttaca gggataggtc tgcactggga tggaatgtta tttttcctct attaataatt
    14401 gttggattca gtgttgtatt tcgaagcgat tcttcgccca ttttgaaggt tggtattgtt
    14461 gcattagcag aaaaaccggc ggaaaatggt atatccaata tacagtttaa tgaattttta
    14521 aagattaaat atataaattt tatccaattt aattctaagg aaaaagcaat cggaagtctt
    14581 caaaaattta agattgacat ggtgattgat agcaacagca aaacatactg gattaatgaa
    14641 tcatcgccta atggatatct ggcagagcag ttgctcaaag gagcacaaaa caagagttct
    14701 gtttttaaaa agcagtcatt ttacaataaa ggtgtacaat atgtagaatg gctttttccg
    14761 ggtattctcg ggatgaatgc catgtttaat tcactatatg gtgttggata tgttcttgtg
    14821 atgtatcgta aaaacggtat attaaaacgt tttagtgtag ctccagtgcg gccatttgaa
    14881 tttttaagtg ctcagatact atcacgaatg tttgtggttt tagctactac agttgtcgtg
    14941 tatgttggaa cagtattgct ctatggattt aagtgcaaag gatcattgtt tacgttattt
    15001 attctcttcg cgttaggtag tttttgtatg atctcattgg gattacttat tgcatcacgg
    15061 agcgatagcc aggaatttgc tgatgggata atcaatatta taacatggcc tatgatgttt
    15121 ttatctgaag tatggttttc actggagggg agtaggccct gggttcagaa agtttcacag
    15181 tttttaccgt taacgcatat cgtagatggt gcaagaatga taatgaatga aggagcaaca
    15241 ctatttgatt tgcaactcca gatagtatat ttagttgcta tctcattatt gtttttattc
    15301 cttggttcat tactttttcg atggtaaaga aaccagtcct atttttagta aagaatacag
    15361 aaaattattt gaattgaaat ataattgaca ctataataaa atgttaaata atgtcataag
    15421 caattgatga tgtaacacat ggtggatgta gctcaatggc agagccctgg actgtggatc
    15481 cagttgttgc gggttcgagc cccgtcatcc acccataata taaacgcgcc cgtagctcag
    15541 ctggatagag cgtcggactt cgaatccgca ggccgcaggt tcaactcctg ccgggcgcgc
    15601 agtgtaatat attcaaacaa tgaacagtac tgttaaagat tttgatatct tcaattatct
    15661 gcctaaagaa gaagattcag tcatcaataa cccgtatctt caggaaatat taacatatcg
    15721 taataatcaa tttgatgaat atcagtactg gaatgtacga tacgatttaa ttaaaaaata
    15781 tgcttttgct gtacctacca ttgataccat aaaaataatt caggagctat cgcccatcat
    15841 agaaattggt gccggtaacg gctattgggc ttatatcctt aatcaggtga aggttgatat
    15901 aatagcaact gataagtacc ctccggacga aacagctttg ccattttcaa attattccag
    15961 caatcaatgg ttccataatc aatggtatga tgttataaaa gccgaagcaa tagaagcagg
    16021 taatcatcct gaaagggcgt tgatgctatg ctggcctgaa cctcaaagca tgatggcgta
    16081 tgaatcgcta tatgcttata aaaatgccgg aggccatact ataattttta ttggtgatat
    16141 gatagcatgc gctgagagca acttttatga tgctttagca caatgtaaac tagaggttaa
    16201 acaacagata tggggatata aatactttaa tgagataatt gcaatataca aaatatacta
    16261 agtattaaga tatatgcggt gttatttatg ttattttgat acaataaaca ctattaatta
    16321 ttgaaatatt aattgtgaga gatagtatga tagaagcgca taaaaaaata ttaattgtat
    16381 caatgcctat ggtagatagt gatttgaaat tatatctggc gccatcacga tgtattgaca
    16441 gtgatgatcc attagtagtt gattttgcac attctgcaat tggttctgaa gcagatgcgg
    16501 taaaaaaatc agtgaaactt ttttatgcag tacgggataa tatatattat gatccctatt
    16561 ccccatttta taaacctgaa cattatcatg gaaggtttgt tttgcaaaga ggtagaggat
    16621 tttgtgtttc caaagcttct ttattatgtg cgcttgcaag agcttgtaat atcccgagcc
    16681 gcgtaggttt tgcaacagta aaaaatcata tagctacaaa acaattactt gaaaccctgg
    16741 gaacaaatgt ttttgcttat catggcttta ccgaatttta tttgaataaa aaatgggtta
    16801 aagctacccc tgcctttaat aaagagttat gtatattgca taaagtatca ccaattgaat
    16861 ttaatggtgt tgatgatgca atcttcccgg aatataattc agaaaataaa ctgtacatgg
    16921 aatatcttga ctatcatgga agtttttatg atgttcctgt cgaaaccatc attacagcgt
    16981 ggaaagaatg ttatggaagc gaacgagttg agttgtggat taccctcttt gaacaaaatt
    17041 ttgctaatag aaaatttgag ggtgaagaga tcgttaaata gtttatgcag atagtgccta
    17101 ttattgatat tttgaaattt attgattgtg attttatttg tgtagatagt ttattggaaa
    17161 ttattcattc caacaatgac tcttttataa ataaaaatat tgctgataca ttcccctatt
    17221 cattaataat tgatgatgta aaaagatatt gcctggaact atcaacaaat gccggtgtta
    17281 aaacagtagt aaaatttcaa aatgaaaatt gtttatatca attttcattg tttgatagta
    17341 aatatggtga taaaaccatt atggttatta ttgcaaaacc caaaaaacaa ataccagaag
    17401 taatacagga catatgccat aatttattta aagtaaagat tgctgatgac cttcaacatt
    17461 ttgttgatgt ttttgaatat attttaaatt caattattca agtaaccgga atggattgtg
    17521 gaggcatcta tattattgat cagcttacca tgaaacttaa acttactttt gccagaggtt
    17581 tgactaactc atatattaaa tctgcatcca tttataacaa cgaaagctgg aactatcagt
    17641 tagtaatgaa tggcacgccg caatttattg attatacaaa catggaactt tgtgacaatg
    17701 attttagacg attggaagga ataaaagctg ttgcaatagt acccataaaa tttagaaatt
    17761 ctataattgg tgttataaat gttggatccc attctacaaa tacaatttct caaaatatat
    17821 atccgctgtt agaagttttt ggtgaacagc ttggtaccat tatggataac tatatggtta
    17881 aaagtgtatt ggctcaatca aaatataaaa aggaaataat tgataccata catgatgtgg
    17941 tgtttcaact ggataaagat ggtatagtac aatatatgag cgggaatata agtactatca
    18001 caggatttac acctgatgaa atacttggta aatattttat aaactttatc tatcctgaag
    18061 atgtggctat gtttatcgaa agttgcgaac tggctaaaag gaggatattt gaaccccttg
    18121 aatatcgatt aattactaaa gagggaagct atcgatatat cttaacgata tctgaatcac
    18181 attatgatga aaagggaaac tatattggat taacaggagt tgttactgat attcatgata
    18241 agaaattagc tgaaataaag ctgaaagaaa gtgatgaacg ttttaaaaca ttagcttcgt
    18301 cggtagcagc tggtattttt gttatcaatg acttcagggt gtattatgtt aatcctgcag
    18361 ttattcaaat tctggaatac ccagaagagg aaattctggg aaaattattt tgggagttta
    18421 tatcagaaga agatagggga tggataattg aacgggcaaa agctcgatta gaaggaaaat
    18481 ctgttccaac ccgatatgaa ttaagacttg ttacaagaaa taatacaatt aaatgggtcg
    18541 atttttccgc tacgcagatc atgttaaacg gtaaacctgc cacattgggc tcagtatttg
    18601 aaattacaga aaaaaaacgt attgaatcgg aattaaaaca atcagaggaa aaattttata
    18661 aggcattcca ttccagtcct atcccaatga ctatcaatac ctataacgat ggaatgtaca
    18721 gggaagttaa cgaggctgca ttaaccacat ttggtttttc acttcaggaa gcattaggga
    18781 aatccatcta tgatctggat gtgtttgcag ataaaaatga gttaaaacaa tatttacagg
    18841 aattacgaac taaccgtgaa gtcaaagata tggatattct tttccagaca aaaaatggga
    18901 agctgaagca gtgtagtata aattcggata tttttatttc ttcaaaggag gaatttgttg
    18961 tcagcttgat tgttgatatc acacagataa agcaaaatgc tcaaattatt ttagatcaat
    19021 ttaatgagtt gcaaaagata aatgctgatt tagctaatgt gcaggcaaaa attaccaccc
    19081 agaatataca attatcagaa gagaaggaac ttttaaatag aatattagag tcaataacag
    19141 atgctgttat tgtaaccgat agttatggta aaatagttct tgttaatact attacctgta
    19201 gcatattagg aaaaccagct aatttgatcc tgaatactat ggcaagtgat ttactcacca
    19261 tagagtgcaa acaatctgtt tcactaaatt ttaaggaaat atatgatact ttgaaaaaca
    19321 atagtattca tcaggaatgt atattacggg ttaatgagag gaagtatgat attgaattta
    19381 atttattgcc attaaaaaat caacacaatg atgtgaatgg cgctgttatt ttattgcgag
    19441 atataacaca gaagaaaatt gttgagcaac aaatggcaaa gacctctaaa ttagaatcaa
    19501 ctggactttt agctgctgga attgcacatg attttaataa tatattaact tctatagttg
    19561 gtaacctttc aatcgtaaaa tataaaatta atgaaaattc agaactatat aatagtatac
    19621 gtggcgccga aactgctgca tttaaagcaa aagaacttac tcagcaatta ttgacattcg
    19681 caaaaggtgg ttcccccctt aaaaaactta cttcattaaa aaatattctt gtagaaacat
    19741 ctacattcgt attacggggt acagccatcg aattccgctt tataattgcc gatgatctat
    19801 acaatgttga ggttgatgaa gcacagatag ggcaggttat acaaaatata gtcattaatt
    19861 cacgtgaagc tatgcaagga tatggcgttt taaaaattgc ggcacataat gttatagaaa
    19921 atgatcccgt acgagttatt ataggtaatg aattatatgt acaaatatca attgaagata
    19981 atggagtggg tatacctcgg gatattttag aaaaagtatt tgatccctat tttacaacaa
    20041 aaccgcatgg tacaggatta gggcttgcgg tagtctattc tgttataaag cagcatggtg
    20101 gttatatttt tgttgattct gaagctggaa aaggttcgcg ttttgaaata tatcttaaag
    20161 ccagtaaaaa taaagctaca ttaacagaga ttccttattg tgatagtata acagttacag
    20221 gctgcggaaa agtattaatt ttagaggatg acagcaatat acagtttatt ttaaaaacaa
    20281 tgctggaaga attgggattt gaaattgata ttacaattga tggagaagaa gcattctata
    20341 aatatatgaa tgcaaagtat aataacaata aatatgcttt tgcaattatg gatttaacta
    20401 ttcccggtaa acaaggtggc aaagatacaa taagacagat acgggctata gataaagatt
    20461 tcaaagttat agtatcaagt ggatattcaa atgatccagt catgtcaaat tactctgaat
    20521 atggatttaa tgcaatatta ccaaagccat atcgattgga ggatcttaaa aaggttattg
    20581 ccacaataat ggcttaaata atattttttt gtttttttaa ataatccggc tattttttat
    20641 tataatagta attgtaaaga atatttattg tagtataata aaaaaaactt gacaccgtct
    20701 ggtataataa actattgctt taatgcaatg agtaaaaaaa tgttctttgg taataaagtc
    20761 aggtaaatgg ttatacgaga aagggttgct agctcaactg gtagagcacc tgactcttaa
    20821 tcagatggtt ccgagttcga gtctcgggca acccaatttc cgctacgctg tgaaaattta
    20881 gcttgaaaaa ttatattgtt tttctgatat tgtttactga tatgcgagcg tggcggaact
    20941 ggtagacgcg ctagacttag gatctagtac cttatggtgt gggggttcaa gtcccttcgc
    21001 tcgcaataag cgggaataac tcagcggtag agtgcaacct tgccaaggtt gaggtcgcgg
    21061 gttcaaatcc cgtttcccgc tcatatacaa taataatccc gataataata tcgggattat
    21121 tattataaaa ggttgtaatt taacccatat aataatgatt tacaactcat aagtctcatg
    21181 gtaattgaat gctggtcata ttataatttt cacaagcata gggaatatta tttcttgata
    21241 tagattcgta gacagcgttt tccttttcta ttgttctttc cgtgtccgcg gtgccttttt
    21301 tatattccac tatccagctt gcctgacttt aaaagttttt ttccaaaaaa ttattgacac
    21361 ttatgctata ttagatatga aggtcaacgt atcatgctgg cgtagctcaa tcggtagagc
    21421 agctgatttg taatcagcgg gttgggggtt caattcctct cgccagcttt gaaggtgagg
    21481 ttcccgagcg gccaaagggg gcagactgta aatctgttgg cgaagccttc ggtggttcga
    21541 atccacccct caccaatatt aaaattaaaa aattagtaag taaaaaacta aataacttct
    21601 ttttggttaa taatcaatct attatttaat ttaaatgaat atgtatcgta taagaaatac
    21661 cgtgttttat gttgaacatt tactttatgg tattatttaa tttttccagc atttcattaa
    21721 tttgggcatc agttaatcca tgtgctctgc ttccctgttc gatagtttca taaattccta
    21781 tatggcaacc aatacaatgc aaaccatatg atgcaagaat agaagctgcc acggggtatt
    21841 tttgaacgag ttcaccaaat gtcatatctt ttgtaatgga tgttttcatt gttataacct
    21901 catatatact tactgctata attattgtaa gctaattata gcaattatat aatatttttt
    21961 catattatta atgtaataat ctttactata tgatagatta aatttaaggt ttaaacatac
    22021 atatatttat ataagcgttt ataataagtt atttttttat tgaatataat ttcatggata
    22081 tctattatgg cattcatgta gtctagaaat acgattaaga aaacatgaaa atctcaacat
    22141 cattttctga gtagctatat taaaaatagt atgaaacaac aaataaagaa tagaaaaact
    22201 attaaaacca ggataatcat ttattgcatt atatgtttaa taattttata taaacatatc
    22261 aatgtattgg ctgatagtta tgcaaatgcc agtttgtctg attgtttgca gattgcattg
    22321 cagaataaca ttagtataaa aatcgcattt gaggaagtaa acggtgcttt atctaatgta
    22381 aagattacaa aagggagcaa caatatccag gtaaatgggg aattgcggac aatagaaatt
    22441 ttaaagtctg atgctgctaa cagctcattc aatgtacctg gcaaggatac attaataggg
    22501 ttatttattg gtgcttcagc aacttataaa ttatacgatg ctaacaaaag taattatgaa
    22561 cagcttaaat tcttagaata taatttagca atattatctg ctttaaagat aaaaaataat
    22621 gtcatgcgag atgtaaagat aacgtattat caactgttgc ttgcaaaaaa gaaaagtgaa
    22681 ttattggaaa aattagtaaa aagttttgaa ataaaatatc tgaatgctga gcaattattt
    22741 acaagtggtc aacgtacaat ggttgatgtt tcaaaagctc aggttgatct gacaaatgcc
    22801 agattaaaat atgagagggc tcaaaatgat gctacaaaaa tcaagagcac attggtttct
    22861 ttaatgggta tgcaatcaga tggctttgat attgacacgg caggatttga tattaataat
    22921 gtagtaaaag agctgccata taattatgat gaattgttac aattagcttt gttgtattca
    22981 tttgacataa aatctgcaga gataaatcag gaactatcta aaactcaaat caatatagaa
    23041 aaggataagc gtttgccaca attaaatgtg gtagcttcgc ttggatatga aaatagggag
    23101 atacagaatg gttcttttag cgaatcatta tctgatagag gtaattggaa accaacattt
    23161 cacgcaggtg ttagtgcaaa tttcccaatc tataccagtg gtgtagtttc ttccagtatc
    23221 gatagagcaa tgatatcata taataaagct atctataatg taaaaactgt tacagatagc
    23281 ataacaagta atatcaaaag ttattatacc acaattaatg atattaaaat tcaattagac
    23341 attgcacaac aattgattga taatgcaaag aagtatcttg atttggtaca aaaaaattac
    23401 gatataggca taattacaca aaatgaattg caggatgctg aacttaatta tattcaagcc
    23461 aatattgatt atatgcaaat tattacagat tattatgtaa attttcatta tttaatcagt
    23521 ttgttgtgta ttgatgaaaa tacattattt tctaaggggg ctgccttgaa atgattaaat
    23581 catttttaac aaaaagaaaa acaatagtta tcattggaat tatattgatt gcgatagtga
    23641 ttattatacg tccatgttct gataaatcgg gatatgaata tgattttggt caggttagta
    23701 ttggggatgt atcaagaacg atatccacta ccggaacgat tgatttactt aatcccgttt
    23761 ccattctatc tcaggtggat ggtgttgtta aaaaggttta tgttgatttt aatcaaagag
    23821 ttaaacagca tcaattatta gcaataatag atgcaggtga tttgaataat gaaattgcaa
    23881 agatgcaaaa gacaattgag cgtcttaaaa tagatcttca aaatacagaa aaagatttaa
    23941 atgttaaaag aagtatgtat aaagaaaata tgatctccaa taatgctttg caattggcag
    24001 aaaatgaata taaaaaaatg caattatcat atagctccgt aattgttgat tacaataatc
    24061 tacttcaaat gctcagggat acaaaaattt atgctccaat aagtggtata attgtttctc
    24121 gcgatataaa tgaatctgat cccgttacac gtaataagag attattttta atggttgatg
    24181 atcttaaaaa tatgagtata acaataagca ttgatgaaac tgatattggt aaagtaaaaa
    24241 cagggcagat cattaaattt actgttaatg cctatcctga tcagatgttt catggtacaa
    24301 taaagcaaat acgttttaat ccaataaata ggggtggtgt gattacctat gatgctattg
    24361 caatatgtga taattcgcaa tatctattaa aacctggcat gacagctatt gttaccatcg
    24421 aagtagaaaa aaagcaaaaa attttaatag taccaaatga agcattaact gttataccgc
    24481 ctactattga tatgtttaag ctggataaaa acaaaaaata tgtatggaaa aagaaagggg
    24541 gtaatattgt gcctgttgag attcaaaccg gactgcttgg agattcatat actgaaatat
    24601 taaaaggaaa tgtaaaaaat ggtgataaga tactaattag agtcaggaaa tccgtgtcaa
    24661 ttaaataatt atgaatagtt aatctatgaa tgcagcaata ataggtatag aaaatttaca
    24721 taaaatttat aaattaagcg aagaaatttc tgttcatgct ttaaagggtg ttaatttcaa
    24781 agtcaaacgt ggtgaatttg tagccataat gggtgcttct ggttcgggta aatccacttt
    24841 catgaatatc ataggctttt tagatacacc aacatcaggg cgatatatac tggatggtat
    24901 tgatggtact tcactaaatg aaaaccaaaa agctgagatc cgaaacagaa agattggctt
    24961 tgtttttcag ggatttaatc tgctttcacg aacacctgca attgaaaatg tagagttgcc
    25021 tctattttat agaggtggat tttctcatga agagatgaga gaaagagcag gcaaattttt
    25081 ggctatggta gggttgtccg gaagggaaac gcatcatcca aataaactgt caggtggtga
    25141 gcagcaaaga gtagctatag ccagagcatt ggttaatgat cctgctattt tactggcgga
    25201 tgaacctacc ggaaatcttg attcaaaaaa tacctctgaa attatgaata tttttaccta
    25261 tctaaataaa gagtataatc tcagtatagt tatggtaacg catgaacctg atgttgcaga
    25321 gtatgctgat aggaaggtta tttttaaaga tgggcttatt attgatgaag taattataaa
    25381 aaagttgaag aaaagaaaaa agtaacatgg atttttcaaa tctgttaata acagcgctca
    25441 gggcgctggc aaagaataag gttcgctcta ttcttacgag cattggtatt gtaattggtg
    25501 tttcatcggt tatcgttatg attggtatag ggcagagtgc cagtatagct gtagaggaaa
    25561 gggtaggtac ctatggtaaa aatgctatgg agatagcatc atatcgtaat ttgcttactg
    25621 aaaatgacag tatttatatc aaaaataaca tacctcaggt aaattatgtt tcaattgtta
    25681 ttacaaaatc aaatgtacag ataaattata aagatcgtgc attgatgagt gcattatatg
    25741 gtgttgagaa tgattatttt aaaatgaaaa tatggccatt gttttcagga aggtatttta
    25801 ccgatatgga aattgatact catgaaagag ttgttatcat aggttcaacg gtaatgaggg
    25861 agattttttt atatgaagag ccaattggta agacagtgat tattaatagt gttccattta
    25921 aagtaatagg tgttctttca gaattgggac agtcatttgg tggaaaagat tttgataata
    25981 ttgtcatcat gccttatgga acagttcaaa ggaaaattga aggtaataga aaaatagatc
    26041 gtatatatgt ctctgtatat tctgagcagc aattggatga aacaattaaa tatatacggg
    26101 attatttaca tcgagttcat cgaatacctc cagatgtcaa tgaattcaga attacaacaa
    26161 gcaaagaaag actggaaatg gctgaatata tttccaagac attggcaatg cttttagccg
    26221 gtatcgcttc aatatcacta tttgtaggcg gtgtggggat tatgaatatc atgcttgtct
    26281 ctgtcagtga gcgtaccaga gagataggta tacgaatggc aataggagca aaacggcgtg
    26341 atatattaag ccagttttta attgaatcta ttacactgag tggattagga ggtataatag
    26401 gtatactatt aggattgttt ttctattata taattactta tatagttgat tggccttaca
    26461 ttttttcttt tacatcagtt ataatatcat tcctattttc atgtgctgtt ggtatattct
    26521 ttggttacta tcctgcaaaa aaagcatctg atttaaaacc tatagaagca ttacgatatg
    26581 aataagtagt atttataatt tttttctcaa atcccttcaa gacataatcc tcttttttcc
    26641 gatattatat acgacataaa aaatttttaa actatatgtt tatgttttga ttttattaca
    26701 taacctggat taacaaattg cgaatcaagg ggaagtatta agaagaggtg atatcaattg
    26761 aaagatatta tagctattaa acggtttaaa cggtttttac aaactgaaaa agggttatct
    26821 cctaattcaa tttactctta tatctatgat ttaaaaaagt tttcagattt tttagcaaat
    26881 aaaaataaaa ctattcttaa tgcttcacaa gaggatatag agcaattttt gttttttgaa
    26941 aaaaataaaa aaagaaactc agcacgtaca cttgcacgat cattggcagc gataaggcag
    27001 ttttataatt atatggcaaa tatagaacat atagccaatc ctacagtgaa aattgaaact
    27061 cctcattttc agaaagcatt acctgatttt ttatctatag aagagataga caggttgttt
    27121 tcttcaatac gtgaggatga tatgtatcaa ttacgtgaca aaacaatatt tgagcttcta
    27181 tattcagcag gattgagaat atctgaagca gttgagttaa aagctgtaga tgttgattta
    27241 aaaaataaat ttattaaggt tatggggaag gggaataagg aacgtattgt accaattgga
    27301 gatgaggcta tacgtttaat agaaaaatat atgagagatt caagacctgt tattttaggt
    27361 agcaggatga gtgaatatct tttcataagc aaaaaaggtg gtatgttaaa cagaaaatca
    27421 gtatggagat tattaaaagg gtatgcgcaa atggtaaata tacgaaaaaa tattactcca
    27481 catacattgc gtcattcatt tgcaacacat cttttagaac gaggtgctga tttaagagca
    27541 gtccaggaat tattagggca tgtagatatt tcaacaacac aaatttatac tcatctagca
    27601 cgaaaacaat tgcaagaaat tcataaacgt tttcatccaa aaggataaca ggtatgcttc
    27661 ttggtatgcc cacaatatgt aagatgcgat tgtatgtaat aattgctctt ttagttatga
    27721 ttggatgtgt cccatcccct gagtatcttg ctaaaacagg aaaacgggtt ggactggata
    27781 atgattatat aagagtgctt ttattgaaag atcaggtaaa agttataata gaaactactt
    27841 ccaaaacaaa agttgtgcaa ttgagtaatg gtactattct ttataatggt aatccaaaac
    27901 atttggaata ttttatccat aaattaccag aaactttgca atttgaaaca tggaataacg
    27961 agattattgt taacggaatt aaagtacgag gaatgattca actaattcct gttgttggaa
    28021 aaatacaggt ggtgaatgtt ttaaaaatga atgaatatct ttatggtgtg ttgccatctg
    28081 aaatcatgcc tggttggcca atagaaactt taaaagcaca ggcagttgct gcacgaacat
    28141 atgcatatta ccatatcatg aaaaataaca gtacatatta tgatttagat gcatcaacca
    28201 attttcaggt ttataggggg gcgatttcag aaaccgaaac tacaaataaa gctgttgata
    28261 gtacatcagg tatcattatg gtttacaata gtatccctat tttagcatat ttccattcaa
    28321 cttgtggcgg tagaacagct gatgatagat ttgtctggaa gggtgaggat ttaccgtatc
    28381 taacttcggt aaattgttca tactgtaaaa actccccagt ctatagctgg caggtcaata
    28441 tctctttaga tgaaatatat gaagcattac taaaaaaata taagactgtt ggtcagatta
    28501 aagctattac tttaggcaga gaagataatc gtgtatcaaa tataaaaata gaacattcga
    28561 atggtataat acggatgaca ggaaatgact ttagaatgat gtttgaaaca aaaaaaataa
    28621 aaagtttata ttttgaagca aatcaaactc atgatgggct tacactgaat ggacatggtt
    28681 ggggacatgg tgttgggatg tgtcaatggg gagcaaaaga aatggcagga cgaggtaaaa
    28741 atttttatga tattctaaaa tattattatc gcggtatttc agttgtatca ataaatacag
    28801 tcccacaaaa atatagaaaa tctaattttt aatatgctgg tttaaattga taaacaaata
    28861 tacttgcaaa taattatgtt ttctaattgt tgggtcctat gaaaaaaaaa atctataccc
    28921 ttagtgattt tgattatttt cttccagaag aacttattgc tcaatatcca gcagaacatc
    28981 gtggaatgtc gcgattatta attgttaacc ggaaggatgg tcagctaata catgactcat
    29041 tttataatat atctcactat ctgcataaag atgatattct tatttttaac aatgcaaaag
    29101 taatacctgc tcgaatttat tgtagacgtt caactggttc aaaagttgaa ttagttttaa
    29161 caaaacatga tacaataaat aatacatgga tagcaatttg caataggtta aaacgactaa
    29221 aagcaggtga aatactttat ccaataggta atgaaaatat aaatattatc ataacagaaa
    29281 aacttgatga tgagataatt attaaaacaa atcccgattt gtcaaatgat atattagcaa
    29341 aaattggtca attgcctctg cctccgtata tacaacgtgc tcctgaacct atggattatg
    29401 agcgttatca aacggtgtat gctaaaaaag aaggtgcagt ggcaacacca actgctggat
    29461 tacattttac tgaagaaata ttgaaacaat tacacaataa aggggttgct attgcttttc
    29521 taacattata tgtttcatgg ggtacattcc atcctgtacg acacgaagat atttcacaac
    29581 ataccatgca ttatgaggaa tactatcttc ctccagaaac agttgatgcc atttataacg
    29641 ctaaatctaa aggtggtaga gtaatagctg ttggaactac ttctttgcga gttcttgaat
    29701 ctgtttataa agaaggggaa tatatttcgg gatatggaga aacaaattta tttatttatc
    29761 cgccttacac aatacaatca gtggatgcac tagtaactaa ttttcatact ccaaaatcta
    29821 cattgttaat gcttgtatca gcatttgctg gatatgattg tatcatgaat gcatatgatg
    29881 aagcaataaa acatcattat cgatttttct cgtatggtga tgcaatgttg ataatataaa
    29941 gaaataaaaa aaattatagc aagcaacact ctcactgtcg gcgttaactc ttcttcaggt
    30001 atttcaggta tttcaggtat ttgtatcttt atgtccatgg ggacatgata atagtactca
    30061 ttaaaattaa tagtcttttt ttatttacct gcattttttg agcagttacc ctttaaaaat
    30121 aaaaaataat taaaaaaact gttgacatat ttttatgtct tctgtaactt gataattgat
    30181 tggatgtcca attaaagtaa cttattacct gattgaatgt ccattcaaat atttattctg
    30241 atattataat aaaatgttaa tgaaaggatg tttgcacact catactacat gttcagatgg
    30301 aacgctaaca ccgcaacagg tagctctcat ttacgaggag aagggttatg attttattgc
    30361 acttactgat catgattatc ttttaaaacc tggatttgag aaaatataca ataacgtcaa
    30421 aacctctttg atgctttttt caggaataga attaacagta tttttaaaag gatatattca
    30481 tattaacaga atatttggag atgtaaactt actacatata tttaatcatt taccagaata
    30541 cgatctcgat attggacaaa ttatgaatag gttatattat cttcagaaaa tgtttcaaat
    30601 agatgcagtt gagataacgg ctaaaggatt taaagtggat ggatttgata caatacatct
    30661 accctatcat tctattgttt cagatgatgc tcatacagag gttggtattg gaagagcatg
    30721 gattgaactg gatgcaaaaa aaaataaaga ctcaatatta agaactatca agcatggtga
    30781 tttttggaat tgctattgct aaagtatgat atagtttaag tattataaat ttttatatgg
    30841 aggttcgtgg gttatggcaa atagagttgc tatctgtgct gttgctcaaa tcaaaaatga
    30901 tcctaatctt tggcatgctc gtttccagaa catgctattg gaatgttttg aaggtatcat
    30961 atcccagaca aatgttggtt ttgatatgga aaagggaatc cgcactgttt taacatgctc
    31021 agatgatgtt tgggatgcgc gtactatctc tgataatgca ataactgatg ttgttggggc
    31081 tcatttcaga ggtgaagaaa aaattgcaca ggaaagtttg aatggtttgg ggtatgcaat
    31141 ggcatgtatc ctttccgggc atgatgatat cattttgtac atggggcata tgaaggaatc
    31201 acaaactgaa agcaggggta tgtgtaccaa tttggcattt gatccatttt attgcagacc
    31261 attaggaatg gattttgtaa atgttaatgc tatgcaggca agggcgtata tggctacttc
    31321 tggtatcaat gaagaacatt tagctaatat tgttgtacgt tcaagaaaaa atgcgaaaaa
    31381 aaatccttat gcacgaaaca atgtattagt taaagaagat gatgtcatga aatccccttt
    31441 gttgtctgat cctttgcgag agcttcatta ttaccctgcg actgattggg cttttgggat
    31501 gcttttgtgc aacgaaaaaa gagcaaaaga atttacagat aaaccggtat ggatgactgg
    31561 atttgccaac tgcattgata gctatttttt aggcgataaa gatttaactt ccaattttac
    31621 tctaaaaaat gcaactgaac gtgcattaaa aaaagccggg atcaaaggat taaaaaacaa
    31681 ttttgagtta tatgaattat gtgatcatgc agcatatcaa ttgcctatgt gggcagaggg
    31741 tattggttta ataaaagatg gagatggtgg taaatggata agtgaaggcg gattggatga
    31801 atgcaatata aacatttcag gaggaatgtt aaatgggaat ccattgcttt taggaggcgc
    31861 ggcgagagtt atagagtgct tttatcagtt aagaggcgaa gcagataaaa gacaggtcaa
    31921 aggtgtcaaa cgtgcattgg cacagagtac ctatggggca gccggtcagc atcaggctgt
    31981 tgtaattatg gaagtttaga ggaggtaagc tcatggcaat agagaaaaat agaaatgtgg
    32041 caataatagg tattggacag acaaagcatt caagtcatag agaggatgtt aatcagccgg
    32101 agatgattta tgaagctgtt tcatctgcat tgaaggatgc taatttaagt atcaaggatg
    32161 ttgattgtgt tgtccatggg aatatggagc tatttgaaat gatccatcag cctgatttat
    32221 ggcatgtatt aggtacgggt gcatatggca aggatagcat acgcataaca actggaggaa
    32281 cagttggagc aacgatagca tgtgttagtg ataaccttat agcttccggg atgtatgatg
    32341 tcgttatggc tattggcttt gagaaactac aggaaggaca tactacaggt ggcattacca
    32401 atatggctga tccattatgg ttcaggaatc tgcaaaccgg agcattaact ggttcaacag
    32461 cgtatgatgt tatgcaggaa tttggagagg atagagcaaa gcgtgcttca atgatgtacc
    32521 gtattattat ggataagcat gcatgcttaa atcccaatgc tcatagagca tttggacttg
    32581 agtttgatca gatagaaaca ttaatgaata catctcctca attggttgga gaactcaggc
    32641 ttatacacat gtgttcgcaa tctgatggtg catgtgctgt tatattagcc agtgagaaaa
    32701 aagcaaaaga gctatcaaaa aaacctgttt ggatacgtga tcatattacc atacaccgtg
    32761 aggaaacatt taatatattt ggctatcacc aaaaatatcc tgtatataaa actcataaat
    32821 ttgctgctga acaattgttc aaacggaacg atattaaaca accactggaa tattttgatg
    32881 tttttgagat gtatgatcca gcttcatggt ggggtttaga ttggttgcgt gatttctttt
    32941 ttctaaaagg ggatgaacat ttaaaactgg tggaaaataa agagatcatg attggaggat
    33001 caatgccaat aaatccatca ggaggagtca tcgctacaaa tcctattgga gctactgcat
    33061 tattgcgtgt tgctgaagct gccttgcaga taagaggaga tgctggagcg catcagatac
    33121 ccaataacgt gaaacatgct ttggcttcag ggtttggtgg gactatgtgg acggttctta
    33181 tgttactaga aaaagaatta aactggtagg agggtgctat ggaatattgg ggagttaaag
    33241 ttgaagatat atttaatact atgcataaca ggtttagacc tgaaggtgcc aaaggagtag
    33301 ttgctgaatt tggttatgat atcattgggg aaggtaaatg gaaaataaaa gttaatgacg
    33361 gtgagttaga agttatcaaa acagatgata tcagtaactg tgttgcagtt atggtatgtg
    33421 atggggaaac gtttgttgga attaatatag gcaaggttga tgctacaaat gcttttacat
    33481 caggtaaggt aaaggtagaa ggtgattttg gtcagtttgg aaaaacagca aagatgttta
    33541 aaaaatttgt actaaaaaaa gctgctatgt ctgttagaga ttatatttta gatatgtttg
    33601 gtacattggt acagcgtttc caacccaata atgcaccaca agaagcaatt atagtatatg
    33661 atataggtgg tgaagatggt ggtgtatgga ctgcgtatat caaagatgga aaatgcgaat
    33721 tgaaagaaga atcacctgca aaattttccg tgaaattaga agtacaggag gctaaagact
    33781 gggtggatgt aatgctgggt aaaacagatg cattttcact tctttctgca gggaaaggtt
    33841 caatagttgg tgaaactcag atagcgttga aattagaaga gatattctct aaatatcagg
    33901 atccaatggc tggcggcact cctgagcagg aacttgttgt attaaaaaaa attatttcag
    33961 ttgatcaaaa attttcaaca ggtccagtaa tgggtaaatt tttaaatgca ttaaagaata
    34021 agaaaatact gggaaacaaa tgcccaaaat gtggccgtat tcaattgccg gcacgtgaag
    34081 tatgtgctga atgtcgtgtg cgtgtagatg aatgggttga agttggccct aaaggtcagg
    34141 ttcgctatat ggaatatgtg tactatgcaa gtcctgaccc gttaacaggt gaaaccaggg
    34201 agacaccgta tggaatggca aatatattat tagatggatg caaaggtaat gatacatttg
    34261 cacatttaat tcgccgtgat cagatagacc tgataaaagg tggaaaagac gatgttagcg
    34321 gtacacgtgt tagacctgta tggaatgagc acagaacggg gagcatcttt gatataaaat
    34381 attttgagat tgatgaatga tggaggaacg atatggcact tgtatatgat aaaaaagatt
    34441 gttttattgt tgaaggaaaa ttagctttac catattctta ctttgcagga agagtaggta
    34501 gtacctttat tacaactcta cgggataaga aaaagataat gggggtaaaa tgtcccgttt
    34561 gtaataaagt atatttgccg cccagacaaa catgtgaacg tgatttaact gatataaggg
    34621 acaattgggt agaggtacag cctgctggtg aagtggttaa ttttactgta gtgcgttatg
    34681 atgacaaata tttaccacgc aaagcaccat ttgttatggc tttaattaaa ctggatggag
    34741 ctgatacacc aatagttcat atccttgatg gagtttcacc ggaagaagtc gtgataggga
    34801 tgaaggttga ggcagtgttt tcatcttcac caacgaacac tattctggat attgcacatt
    34861 ttgcaccaaa gaaaccagaa aaagtttatg tttcccaacg caaaacaggt gcaaaagaaa
    34921 aagagggggc tcaaccaatt tctgatgaag aaaaattaaa acaagaaagg aggaaaggaa
    34981 tgtcaaaaaa ggttattatt acagcagcat tatcaggggc tgcaactatg aagaatcaaa
    35041 atcaagcagt gccctataca cctcaggagt ttgcagaaga atcatataaa tgctataaag
    35101 caggtgcagc tatgattcat gtacatgctc gtgaagataa tggtatgcca acacatgatc
    35161 ataagcgtat taaagatacc catgatgcta taaaagaaaa atgtcccgag ttgattgtta
    35221 atttaagttc agcagtgggt atgggtaaaa cacctgaaca acgtataagc cagatacttt
    35281 atgtaaaacc tgagatggct tcactgaata ccaatactat gaacttcagt attgtcgata
    35341 gaaaaactgg taaagtattt atagacttta tatttgaaaa tacatttacg atgttacagg
    35401 attttggcaa agcaatggaa gaaaacaaca taaagcctga ggtggaagta tatgatcttg
    35461 gtgggcttga taattggttt ttgatagcta agcagggatt ttttacaaag ccatataatt
    35521 tcaattttgt atggggtgtt gcaggaggca tggaatttag gcctgatatg ttcatggttt
    35581 tgaagaatgc attaccggag ggtgcaaatt ttacaacatg tggtgttgga acagaacagt
    35641 tcccttcaat aaatttgtca tgcatggttg gaggacatat gcgggttggt cttgaagata
    35701 atattagaat tcctaccggt gaattggcaa agggaagtta tgagcaggtt gaatgggcgg
    35761 taaaaattgc acaaatattg ggtagagaac cagctactcc ggatgaagca cgagaaatta
    35821 tgggtctgaa aaaacaataa tatttcttat ccctgcatct gcttattatc acggatagat
    35881 gcagggaata tctattttgt acgatagtta tttgtaaact actggcacat tgagaaatta
    35941 cgattatacc aatgatgtga tgacattagc tatttgatga ggagatgtga tttgatagag
    36001 tttgggaatt ttgagtaacg atgattttaa aaatggttaa tggaagcata tggtttttat
    36061 ctgttagcat accattaatg atgatggttt tagttaagaa gttgttcatg gccagcgtgc
    36121 aaccacctat aaggaatgaa aataggttgc atgacaaacc ccgtaaggat gacattatac
    36181 tatcagtatt gacgattaca atgtagattc cgggtcaagc ccggtatgac gttgagtagg
    36241 acgtcatcct gaatttattt caggatacaa cgttacatgt tgagattcca gattaagcat
    36301 ggaatgacag ggatgcatat tacataggta tggatgagat aatgcgtact tttactatat
    36361 gcaattaaaa ttcaggattg aattatgaat gataaatgtt attatcagat agaaaaagaa
    36421 aatgttgctg tagtttattt taataatcca ccaattaatg ctttagatac tgaaacaatg
    36481 gaatcgtttg aagaaattat gaggatattg ataaccgatg ataaagtgca tgttatcgtg
    36541 ctcacgggga aaggctctac atttatagtt ggtgctgatg taaatgaagt cgcaaaagtt
    36601 aataccttcg ataaaggggt agaaattaca acgaaagcac aggcaatagt ttcattatgt
    36661 gaactatcgc ccaaaccagt tattgcaatg attaatgggt tgtgtttagg tggtgggatg
    36721 gagatagctc tttcctgcca tatcaggatt gcaagtaata cagctcagat gggtttgccg
    36781 gagataatgc tgggtattat tccggcattt ggtggaacac agcgtagtcc acgactgctt
    36841 ggcaaggcta aagcactgga gtttatgtta acaggaatgt ttattaatat gcaggaagca
    36901 ttacaaatag gattggttaa tagagtttat gctcccgatg aattaattga agaaagttta
    36961 aagtttgctt cttcaattgc aaagaaagga cagattgctg tacgatctat tgtagaagca
    37021 gtcatgaagg gtagctcaat gaatctggaa gatggattac gattggaaag taccctgttt
    37081 gggaggttag ctgaatcaca tgataaaaaa gaaggtatcg atgcattttt aaataaaaga
    37141 aaaccaatat ttaaacattg ttaagaaatt ggaggcaacg catggaggaa aaaacgatta
    37201 atcaggtttt taggaataga gtgcaaaaat atggttcacg catagctctt gaaaaaaaat
    37261 taaatggcaa atgggaaagt gtttcatggg aagattatta taaaagggct gcatatgttg
    37321 gattggcgtt aaatgacatg ggattgcaaa aaggtgatag ggtatcattg ctttcagaaa
    37381 accgtttgga atggctttat gctgatatgg gcgttctggg agctggaggt tgcatagtcc
    37441 ctatttatcc tactttaatt gatgatgaag ttgagtatat agtttcccat tctgattcaa
    37501 aatttttaat agtagaaaat aacgctcaat tacaaaaggg tttgtatact gcaaagcgtt
    37561 atcctggttt aaaaaagatt attgttatag atacaactga agttaatgtt aatcccatga
    37621 ttatggatta taatgagctt cttgataaag gaaaagtgtt atataataaa aatcctgaag
    37681 catttgcatc attagctgat actattgctc aggatgatct tgcaaccata gtgtatacat
    37741 caggaactac cggtgttcct aaaggagcta tgattaccca taaaaatatc atggcagtta
    37801 taaaggcact tgataccatt gaacctcatt atgcatatga tagtgatcaa actgtaccat
    37861 ttttaccgct tagccacgtt tttgaaagag tagcagggca cttttatgga atgtatgtag
    37921 gtataactgc atcatatgca gaaagtttag atacgttaat acaggatatt caggaaaaac
    37981 ggccaacagt tgtgctggca gttccccgtg ttcttgaaaa ggtatatcag caaataatat
    38041 cacaggtaca ggagcaatca ccttttaagc aaaaggtatt ttattggggg caaaaagttg
    38101 gcagtaaaat cagtgaattg agagagcaga aaaaaaatcc aggatttaat ttgtccttga
    38161 aatataaaat agcatatgca ataattttta agaagttgca aaatgcatta gggggaagag
    38221 tccgctggat gacagcttca ggcgctccaa cagcaagaga aataatttta ttttttaact
    38281 cagccggaat tatggttatt gaagggtatg gaatgaccga atgctgtgct ccagcaacca
    38341 tgagcaatat tgctgattat agaattggaa cggtaggcag accattgccg ggagttgata
    38401 tcaaaattgc tgatgatggt gagattctta ttaaaggtga taatgttttt aaaggctact
    38461 ggaagatgcc tgaagagaca aaagactcat ttactcagga tggatatttc atgagtggtg
    38521 atataggttt atttgatgat gctggttttt taatgattac agacaggaag aaggatttaa
    38581 ttattacttc aggcggcaag aatgttgcac cacaaaagat tgaaaatctt attaaatccg
    38641 atcctctatt cacagaggcg atagttattg gtgataagcg aaagtattta attgcactgg
    38701 ttaatataag tcaagaacag gcagagcgaa ttgcaaaaga aaagggtatt aatgttaata
    38761 gccttcatga tcttttcaat aatccacgat ttcaaacgat agttgaagaa catatagaaa
    38821 agtgcaatca aaagctggct cgatatgaaa cagtaaaaaa agtgggcatc ataaagaatg
    38881 aattttccaa agaaacaggt gaacttaccg ctactcttaa agttaagcgt aaagctgttc
    38941 agcaaaaata tcagccaatc attgattcct tatatgagga ggatgcaaaa actattcaga
    39001 tttattaaaa gcgtatatta ttgattagat gttcaatcaa tttatgaatg tatgctttat
    39061 tttgaatgat aagcaggagg ttactatggc attaggtact cgggataacg atacggaagg
    39121 tgtaatgcta tcggggaaaa atgaactgtt taccgatcct gatcccgata aagccagagc
    39181 gtttttcaga aataaatccc gttctatgac acctaagttg gcacagatta aagatgttgt
    39241 ccgggatatg attaaggatg gtgattatat tgccattgga ggttttggtg caaaccgtat
    39301 acctacagca attgttcatg aaattgtgcg tcagcataaa aaacatctgg gatttgccgg
    39361 tcacacgtca acacatgatt ttcaggtgct tatagcgggc aaatgtattg atcgctgtga
    39421 tgttgcctac atcataggat tggaggctcg aggactatcg cccaatgcac gtcgtgttgt
    39481 tgaaagcggc acaattaagc taaccgaatg gacaaacgct tcactttcat ggcgactcaa
    39541 agcagcagct atgggtatat cttttatacc tacccgtaat atattgggaa cagacacatt
    39601 taaacacagt gctgccaaaa cgataacatg tccatttacg ggaaaaaaat atgcagccat
    39661 gccagccttg tttccagatt ttgccgctat tcatgttcat gaagcagata tctatgggaa
    39721 ttgcagggta tatggagcat cagtatcaga ttttgatctg gcaaaagctt caaaaagagt
    39781 ggtaataaca acagaacgga ttataccaca tgatgaaata cgaagagaac ctgaaaagac
    39841 tgtcattcct tactggtgtg ttgatgcaat tattgaagta ccgtatggca gttaccccgg
    39901 taatatgccg tatgaatatt ttagcgatga agagcatctc aaaacatggc tgaaagttga
    39961 acgagatcct gaagaattag aaatattttt gcaggagcaa atttatggag tgaatgattt
    40021 ttatgaatac ctcaatttga atggtggtat tgaaaaaatg aaaaagctca gagccataga
    40081 atatctgatc aataataann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
    40201 actgtgctgg cgtacgatga tgatgacggt gcaggatgct cgcaggttta ccgataaact
    40261 tgattttata acttcaccgg gctatttaaa aggtggcaga tcacgatatg atgccggctt
    40321 gcccgaaggt acaggacctt accggataat taccaatatg gcagtaatgg gatttgatga
    40381 tgaaacaaag tggatgatgg tactgtcaat taatccaggt tatagccgac aggatgttca
    40441 ggataattgt ggttttgaat tgaaatgggc aaagaagata gatgacacca aacctccagc
    40501 ggatgaagaa ttgcggatat tacgtgaaga ggttgatcct catcattata ttataggaag
    40561 ataaaaggat aaaaatatgg gaaatatatt tcatggtatt gaaccatcgg tatataaaag
    40621 tcagatcaat gttccctatc agtggtgggc aggtgaaact gcatctaaat attttatagc
    40681 attaagggat gaaaaaaaaa tattggggaa acaatgccca caatgcggta aagtttattg
    40741 tccaccaaaa aaaacctgcc cttcatgctt tactgaaacc aatgcatgga aagaggttgg
    40801 tcctaaagga aaattgattt catatactat agttttacga caactggcag ctttacagaa
    40861 aaaagttccg gttgcttttg gtttaatttg tatagacggt gcggataatg caatgcttca
    40921 ttatattggt gatgtggatg taaataacat tactataggg atggaagtag aggctgtttt
    40981 tgcagatgat agaaatgcaa caattaatgc catttcgcat ttcaaaccgc tgacttagga
    41041 ggaaaagtat ggatgcagct atcattggta taggaatgac aacaatagaa aaaaacaaaa
    41101 tagcctgtaa ttatgcagat ttagcatggg aagcggtcaa taaggcatta gcagatgcat
    41161 caataacaat agatgatatt gataatgtga taacaacctc aagcgatttt tgggatggca
    41221 gaaccatttc atgtatggcg gtaggagatg cctgtgctgt acggcataaa aattgttcgg
    41281 cagttgaagg ggatggaact tttggtgcag catatggatt aagcagaaca ttgtcagggg
    41341 attacaaaac cacgctggta actgctcata gtaaaggttc agagagtata tccagcatca
    41401 taaccaatgc tgcatttgat cccatttatg aacgaagctt aggactggac atgataagtg
    41461 ccagtgcaat acaggcaaag cagtatatgc atgttacagg aacaacccct gaacaattag
    41521 cccttgtttc ctgtaaaaat cacaaaaatg gaaaggataa tccttacgcg caactgccaa
    41581 tggatattac agtaaatgat gtactcaatt ctgaaatgat tgctgaacca ttacacaagt
    41641 tagattgttc tccggtttca gatggttgtt gtgccattat catagcaaat gcagagtttg
    41701 ttaagaaagt aaaagctaaa ccggtgtgga ttaaaggagt ggctttttgc agtgatgcgt
    41761 attttatggg cgacagagat ttaacaaaaa ctatcgcact ggaaaatgct gctaaaaaag
    41821 catatatgat ggcagggata accaatccag aaaatgaaat tgatattgct gaaatatacg
    41881 atgcgtttac ctaccaggag ttgatgtgga ttgaagggct tgggttggca gaatatggga
    41941 atgctggcaa acttttagaa aaaggaattt ttaataaaaa tggtaaatta ccggttaatc
    42001 catcgggtgg attgttaagt ggacatccag tcattgcagc aggtttgtat agaattgcag
    42061 agattgtttt gcagattcgc ggagaagcag gtgcacggca ggtaaatgct aaacgtggca
    42121 ttgcccatgg tgttaacggt atatgtggtc aatcacactg tgtatgggtc ctggataaag
    42181 aaaaatagga ggatggtatg gctaagcgca gagttgcaat tgttggcaca ggccaaacta
    42241 atcataaaag tcatagacct gatgttaatg gacaggaact aatacatgaa gcggtagtta
    42301 gagcattaca ggatgcagaa ctgtccatta atgatatcga tgccattatt attgggaata
    42361 tggatcactt tgagggtatt aactatgtag attgctggag tatagatggt tcaggtggtt
    42421 atcataagcc tattatgaaa cttacaactg gtgggactac ggggagtaca cttgcaattg
    42481 gtggatatca tcttgttgca tcaggaatgt ttgataaagt tcttgttata ggctgggaaa
    42541 aaaattctga atcagatact accggtgcta ttaccacagc atttgatcct atatgggaca
    42601 ggcttgtatt tgcaggagct atctccggat tggccgttga agcgcaaagt tacatggcaa
    42661 aatatggtgc cactgacaag gatggtgcgc gtgtttctgt tcgtgaccga aagcatgcgt
    42721 gcaataatcc atatgcccag ttgcgaaagg aggttacctt agaggaggta ttagcatcgc
    42781 ccatgctggc agatccaata catcttttag atgtgtgtcc gcgaactgat ggtgcgtgtg
    42841 cagttgttat ggcatctgaa gattgtgcag aaacaataac atcaacacct gactggattt
    42901 gggcaacggc aaatcgacat acgtattcat atttaggaga tgtcaattgg ggcgagctgg
    42961 atagcatagc tgatgcttca aaagagcttt ttaaaaaatc tggtatcagt gaaccaagaa
    43021 aacaaattga tgtcattgaa atgtatcagc cgtattcact ggcaggaatt atatggattg
    43081 aagatatggg gattatccca cgaggaaaag gtgctgaata tatatggagc ggagcaacag
    43141 atatgggtgg ggaactcccc atcaatccgt caggtggtgt tattgcatgt aatcctattg
    43201 gcgctacagg attaatacgc tgtgcagaag ctgccatgca ggtcatggga aaggctgaag
    43261 gaaggcaggt tcctgatgtt aactttgctg ttagcactgg ctttggtggt tgtatgtgga
    43321 cagacatgat actacatggt aaaaacaaac ccaatcttta ggagagaaag tatggaaaac
    43381 cttgagcata taattataga atctggaaaa gctgtacaac cttttcacta ttcagtgggt
    43441 atgtacggca gtaagttctt tcatgaactt aaaaataaca gaaaatttgt tggagttcga
    43501 tgtcctaaat gcaaaaaagt atatgttccc ccgcgtcagg tgtgtggtga atgttttatg
    43561 gaaatgaagg attttataga ggtaggacca aaaggaataa tcagtacatt tacaatcttg
    43621 cggtttgcgt ttatagatcc ggaaaccgga ctgcaaaaac ccgtgccgta tgggtatgga
    43681 tttattaagc tggatggtgc tgacacactt ttccaacatt ttattgatgt aacagaagaa
    43741 tctaaagtaa agattggtgc acgtgtagaa cctgtttttt cagatgaaaa gaaaggcacc
    43801 attaaagata tattatattt caggatcatt gattaacata tgagtttgat agaagtaaat
    43861 aatgatatgc gggtaataat ggacagggaa gctttttttg ttatgctttt ggccggtacc
    43921 gataagatta gatctggata cggtaatggc gatagtttgt taattgatag tgagcatagt
    43981 atatttgcta ttgctgatgc aaccgaacga tttcccaatg catcacgcat attgctttca
    44041 aaaattatgg gaggtttagc cagcattcct gatacttctg aacagtggcg tgcctatatc
    44101 aatgcaatat accgacaaca ggaataccat ttaaaaacca cattgagttt gatagcacta
    44161 aaacagtcac ataatggatg caaagcattt gtagtacatg gtggcgatag ttctttaaag
    44221 atatttaatc gcttaaataa aaaactttta tataaatcaa cagccaatat gaattttgca
    44281 gggagaagta catctattga taccattgat tccattaatt tagaatcaaa cacacgaata
    44341 attatacata gtgatggatt taatgatatc atgaagattg gcgctattga ttttgatgat
    44401 attattgctg ctccactatt tgattgtata tcattaattt taagccaggt aaatgctaaa
    44461 aattttaaca atgaatatga tgatgtggga ttattgatag ttgatgtacc tgaaatattt
    44521 aatgttatat ctggtggtat tctaatgggt ggaacaactc attgtgagga acgatgtttg
    44581 cagaataatt taaccatatg tgattatagt tctcttataa aaactttttg aatatggtat
    44641 agtttataat tgattggtca ttcaatcaga atgtagagga tactgatgag agttaagcag
    44701 gaacgaaaca gcatatatga tacacggcgt ttacagctta ctgctgcagc atataatgtc
    44761 gtgagtaaaa aaggatacta taattttact attaaagata tagcagatga agccgggatg
    44821 tctgcagggt tggttcatta ttattttaaa aataaacagg atttgctgct aaatcttctg
    44881 cgtcagataa ataccaatat acgattggca ttgcaaaatg atctggctaa tatcgaagat
    44941 ccagttgata aattgagtgt ttttatactc agggcatgta atctcataat tgaagaaaaa
    45001 aattatttct atgtactttt agatttttgg acacagctca accataatga acgtatgagg
    45061 aaagcaatac aaaagttata tcaaagttac cggaaagtat gctcggatat actcaatgaa
    45121 gggataatta agggcaagat taagcaaatt gatgttatct atacagctac gatgattgtt
    45181 tccatggtgc agggtttaat cattcagtat gttatagaac ctgaagcttt tgattattca
    45241 tactatgtaa ataaaataaa aaatcaaatt attgaaacaa taatttcaaa gggggtaaac
    45301 aatggaattt agtttttctg aggaacagct tttattcagg gacacaatat ataaatatgc
    45361 aaaaaatgag atagtaccac tatgtgaaga agctgatttg aaatcagagt tttcatttga
    45421 aatctggcaa aaattagcta ggatgggatt attgggatta ccattccctg aagagtataa
    45481 cggacagggt gctgatgttg ttacatgttg tctggcatca gaggctttag gtcatgcagg
    45541 cgttgatagc ggccatcttc tggctttagg agcccatacc tatttatgta ctgatacatt
    45601 atttaaacat ggaaatgagc atcaaaaaaa aacatatgta cataaattag caaatgggga
    45661 atggatagga tgcatgggat tgacagaacc aggtgctggc tctgatgctg cagcgttaca
    45721 aacaacggca gtaaaaaaag gcgatagatg gatattaaat ggttcaaaaa catttattac
    45781 caatgcacct gttgctgatg tttgtgtggt gtatgcgact gttgataaat caaaaaaaca
    45841 cgctggcata acggcattca ttgtggaaaa aaattttaaa ggattttcca ccgggaaacc
    45901 ttttcataaa ctgggggtca gagcatctac tacttcagaa atatttttag aagattgtga
    45961 aatccctgaa gataacgtat tgggtaatat tggcgaagga tttatgtatg ctcaccaaac
    46021 gctggaatgg gatagaagcg ctttactggc tccctttgtt ggtgcaatgc agtttatgat
    46081 agagcaatgt gcacgatatg caactgaacg agttcaattt gataaaccta taaaaagctt
    46141 tcaggcaata cagcataaat tagctgatat gaaaatggtt atggaagcag cacggttagc
    46201 ggtatacaga gttgcttcat taaaggatgc aggatatcca ataaatcatc tccaggcttc
    46261 tatcgccaaa gcatttgttg gtgattgggg ctttaaagta gcatctgatg ctgtacaaat
    46321 ttttggtggg tatggctata ttcatgatta tccggttgaa aggatgatac gggatgcaaa
    46381 attagctcaa ataggcgggg gaacatctga aatacagcgg ctaataatat cacgtctgtt
    46441 aacattgtaa tatagttagg tcaggaggct attatatgaa ctatgagtta aatgatatac
    46501 aaaaatcaat aaaagaaaat tttaaaaaat tttgtgaaaa tgaaataaaa cccaatgcca
    46561 gtatcctgga tgcaggcgat gaaaaagctg ttgagttatt gaaaaataat attaaaaaat
    46621 taggtcagct tggttatatg ggcatgatac atgaagaagc atttggcgga actgatcaag
    46681 accttattaa ttttgcaata gctggtgaag aagtttcaaa agcatgtgct gcaacttttt
    46741 tatcagccgg tgcaagttgt ggactatgtg gtatgcctat tcggctattt ggcaatgata
    46801 agcaaaaaga aaaatattta cccggtatta tatcgggaga tgtaattggg agctttggat
    46861 taaccgagcc gcaagccggt tcagatgcaa cagctatagc tacaacggca aaaaggaaag
    46921 gtgctaaatg gattttaaat ggtactaaaa catttataac caatgccccc attgctgatg
    46981 tatgtcttat aatggcttat aatgataaag aagccggtcc tggtgcgggg gttactgctt
    47041 ttttggttga tacgcataca ccgggatttt ctattggaaa gccatttgac aaattagggt
    47101 atagaggttc gccgacatct gagatatttt tagaggattg tgaagtttcg gatgacgctg
    47161 tcctcggtga attgggtaaa ggatttattc aggctatgca aactcttgaa tatggaagaa
    47221 taggtatggc gacagtttct cttggagtgg ctgttgcctg tatggatgag gcggtaaagt
    47281 atgcatctgt gcgaaaagca tttggtaaac caataaaccg ttatcaggaa atatcattta
    47341 aactggcgga catgatgata ttaactgatt tatctcgatt actgatatat aaagctgcct
    47401 gggccaagca aatgaatgat cctgaggcag cagtattggc ttcatgtgca aaactgtttg
    47461 ccagtgaagc agcaacacaa atagcaagct gggcactgca agttcatggt gggtatggct
    47521 atattaagga attccctgtg gaacggttat accgggatgc aaaattgggt gaaattggtg
    47581 aaggtacttc tgagatacag aggatactca tagcacgaga tattttacaa aaatatggaa
    47641 gctaatggag gagatatcta tgactgttga taaaaagttt attggtaaaa catggccaac
    47701 agtgatatat gaagtgggta aagaaaaaat aaaagagttt gcacaagcaa taaagagtaa
    47761 caatccttat tatttcaatg acgaatttgc tttacaatct aaatacaaaa cgatagtagc
    47821 accaccaacg tttgctgtag tgtttggagc aaaactgata gaaccaatat ttatggataa
    47881 tgagcttagt ttaaacatag ctatgcttgt gcatggtgag caggaatttg aattttatac
    47941 catggtaaaa gctggtgata ttttatattc caatgccagg attattggta ttgaaaataa
    48001 agaaaagtta gacgtagtga caattgaaat catgacaaaa aatcagtaca atcaggatgt
    48061 atgtaaagga atatatactt ttgtagtgag gaaataagga tgaaaacaat gtatgaaaaa
    48121 aacaatatag atgctggaga ctcttttaaa gtacaacagg agattgataa ataccgagct
    48181 atttactatg ctggtgcatc aggagatttt aatccaatac atattgacgc tgaatttggg
    48241 aaaatggttg ggttgggtgg tgcaatattg caggggctgt gcactctggg ttttactgct
    48301 gatgcagtta ccacatggat tggtgaccct ggcagattaa aaaaattaaa atgccgattt
    48361 tcgttaccgg taaaaatggg tgatatcata accatagaag gattggtaac agaagtgcgt
    48421 ggggactctg gaacagtgga aataaaagtt attaatcaaa atgggagtga tgttttaaca
    48481 aatgtacagg ccgaggtagt ggcaaaataa attgtatggt attgctgcta tctttgcaaa
    48541 ctattctttt aatccaggaa aactattgat aaggtggttt cacaatcttt tgaaaatagt
    48601 atcgcaatgc atcatttcat tcttagaaaa taggcttatc aataacaggt aaaagatatt
    48661 cattatatag tgtaaaggtt ttgtcatcct taaataatga aaagaacttt tttttcaaca
    48721 tttcagattg tagtgaagtt gtaaaaatat tatttcgttc atcactatca tttgataaat
    48781 catacagttc atattcaatg ccataatggg ttggcatata tatcaattta taattgtttg
    48841 caattatgca tcgatgcttt gcaatagtaa tacaatattc ataatatgga gtaatggtaa
    48901 tctcgtcatg gtagtgaaaa tcaattgttg agataccggt aatatccggg tacattatac
    48961 gctttttctg aaaaaagaag ttaccggtat tatcaaacca tatacctgtt tcacagtatg
    49021 catcaaaatt gcgtttgtgt ttttctaaaa gggatgtgcc atcaaatgat tctggttttt
    49081 gaatacccag tagatcaagg atagtgggtg ctatatctat agcacttcgt acagcacggt
    49141 gatgttggta tggtataccc ggacctgcaa agatacaggg gattaccatg ctatacttac
    49201 cacgcagatg ttcaccatgt cccatgccgt agtcatattc gtaaagattt tcaccatgat
    49261 ccgaaaggat tatgatgatg gtgttgtcat atatattttt atctttacaa tactgtataa
    49321 cctttccaac agcttcatca aaagcataga cacaaccatc atataatgct ctaatatatg
    49381 caacctcgtt atcagaagga gagtattcat gtgcttcacc ttcggaatca agtgatacca
    49441 ccctgttttt agaatattta aatggaccac tatagtgcct gtcagtaaat aaattatagt
    49501 agggataaca tgcagggtaa ggaaaatgcg ttattgaaaa aaatgatgtt ataaaaaatg
    49561 gcttttttgt ttttatactt ttacttattt cagtttttat atcctgaaca acaaattcgg
    49621 ggtcagcaaa ttcggcaaat tcttttatct ctggaaaaca ataaaaacca gctttattgg
    49681 ttaaaaatgg caaaaggaac gtatgatttt ttaagatgat ctgttgtatc ataacacgca
    49741 tattaaatgt tggtgctttc actgtttcgt agcccaaatc aatacgcggg aaaatatcag
    49801 cagcaaagtc gccaaccacc gatgtatagt aaccatggtg tgacaatatt tttggcaatg
    49861 ttataaaatt ttgttttcgg gtaaccgcca ttggaaacat gtggctaata tgatgtttca
    49921 tgggtagctg tgatgttata atactaaccc atgccgggaa tgtacgcggt acaacagtgt
    49981 aaacattgtg aaaatttgct ccctgttgta ttagcgtgtc gatagtgggt gttgttttac
    50041 gataataacc atacccgcta aaatggtcgg ggcgcaatgc atcgctggct aaaataataa
    50101 tgtttggttt ggtggtggtt ttattatggg cgatagttat tggtaataaa gatattgtta
    50161 caatagtaac tatcacacat gcaacaatca tttgcttttt tttattgcgt aacgatgcca
    50221 tctgctgaaa atatattttc atatgattaa ataattgtct atacacaacg aatacaaaag
    50281 cagcaatgaa tggcacaaca atactgcatt gcaacgccat aaaaaaatca ggagaaaagt
    50341 tattggttaa aagtatttga aaatttttgt agattatatt tttattgaag atggtatcca
    50401 gatataattg cggatacatg acaatgcttt tgcataattg caaaaatgca ataataaatg
    50461 tcaatgccgc agttaaaaaa cttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50581 nnnataattg taaataatta aatattgatt aattacatta tattggatgg gtcattatat
    50641 atattttagt taagcatgat tatatccaat acataatggt atttgaaata aatataaaaa
    50701 tatcacaata taatagaaca tgttctatta tattcttgac aatttctacc attttaagtg
    50761 attatcaata taattgggaa gtatagactt tcccattata ttaaaaaatt aattataaac
    50821 ggaggtaatt ttaatggata caaatgtttt actaaagaaa aacaattatg ttgcaactat
    50881 aatacttaac cgtcctcctg caaattctat caatattcat gtcagagaag agttgtatgc
    50941 tatagtaaag gaattagaag atgacaatac tattagagtt ctgataataa ccggtgcagg
    51001 cgataagggt tttagcgcag gtatggatgt aactgatgta gcaaatctgg ataaaggtcc
    51061 tgatgcaatt gaacttttta attatattga tcgatatcca aagccggtaa tagcagctat
    51121 aaatggctat gcattgggtg gcggttgtga attagcgtta gcatgccatt ttagattgat
    51181 gacagatatg ccaaaagcat tcattggttg cccggaagta aatttaggta ttactcctgg
    51241 ttggggaggt attcaacgta tgacccgcct gttaggacgt agcaaagcac ttgattgtat
    51301 actttttagc aaacgattaa ctcctcaaga agcattatca attggtcttg ttgatcgagt
    51361 gttcccacat gaacacttta tggaagaagt acaaaatttt gctgattctc ttgcaagacg
    51421 tgcaccatta gcggtaagct gtatcctgaa gggtatgata actggtatag agaaaggtat
    51481 tgatgagggt ttaaaagtag atcggcaatg ctcagctatg ctggcaaaaa cagaagatgc
    51541 agcagaagga tttatggctt ttgctcaaaa gcgtgagcct gtttttaaag gcagataata
    51601 aatagatagt aactatcgcc tactaattgt aatgcaaggt ataattaatc ttcaaaataa
    51661 ttttcttgca tggatacagt aagtccatca gagccacgta tgattttttc taaagcgtag
    51721 ataccagtaa gttcataacg gaagaaatat gcaagtgttt gcatctttcc ttcaagaaaa
    51781 tccttactgt agcttcctga actgttgtta atactatgtt gcacagctat tccctgtaat
    51841 aaccattgcc atgctatgca aatgagaccg ctaaattcaa gaaaaagagt agcgtcggca
    51901 agaaaacgtt caatatcacc tttaaccgca aattgaatga gaaagccggt tacctcctgc
    51961 cataaagctg cagctttatc caaatcttca gcatagctga taagcgaagg gtattgtttt
    52021 gctgaagcga tagttttgta aatttcagtc atatatactt tagcggtagc accgtttttc
    52081 atcgtgactt tgcggccaag aaggtccatt ccctgtatgc ctgttgtgcc ctcatggatg
    52141 gggtgtatgc gtgagtcgcg catgtattgt tctacaggaa actcgcttga ataaccatag
    52201 ccacccatac actgaatagc taaacttgta gaaacgacgc ccatctctgc aggataggtt
    52261 ttaactatgg gtgtgagcat atctaaaaaa agttcatact tttctttttc ttcgccagca
    52321 ctggtattga tgagatccac cagtttacct gtttgtatta ttaatgaaag tgaaccctct
    52381 acgatagctt tttgctttaa aagcatcctc ttaacgtctg catggtgtat gatgggtatt
    52441 tgaggaacag aaggattctt ttcagtaatt ttacgtccct gacggcgttc ctttgcatat
    52501 tccagcgatg tctggtaggc agctccggct atggcagcag ctcctaaacc cacattaatt
    52561 ctagcttcat tcatcatatg aaacatatag ctaagccctt tattgggttc gccaacaagg
    52621 tatccacggc agtcatccgc ttcgcccata cttagctgtg cgataggggc tcccttatat
    52681 cccattttat gataacaccc cgcacagttg acatcattga aaataagttt tccgttttca
    52741 tccggtctat attttggaac aatgaaaagt gataaaccct ttactccttg tggcgcatcc
    52801 ttaatacgtg caagcatcag atgaacaata ttttcaacag catcatgatc accagcagat
    52861 ataaatgttt tctggccttt tatgagatag tatcctttat cagtaggtat tgccatggtt
    52921 tttacatcgc ttaatgagct tcctgcctct ggttcggtta aagccattgt gccctgccat
    52981 ttaccggcta tcatgggcgg tgcaaaaatc tggtaaagtt cctttgttgc aaattgataa
    53041 atgagtgcga gtgctcctga tgtaagaccg ggataaacac tcattgaata atttgcggag
    53101 gcaaagatga acatacaggc attaaaaagg ataaaaggca tttgttgtcc cccaatgtct
    53161 gaaggaaacg atgcattaat ccagccaccc tctgcaaact tttccaaaaa gggttttact
    53221 gaaggatgaa ctttgatggt ggaacctgtg tattccggag ggtttttatc catttcggaa
    53281 aaatatggat acatcatgcc cgaagctatg ttttttgctg tatctaaaat gatgttcatg
    53341 ctttcaacgt tgtaatcact gaaagtatcg caagaaaaaa tttcatcaat gtcatgaact
    53401 tcttgtaata aaaatttaag attttcttcg ctgtaaaatt ttgttcccat actcaattct
    53461 ccttcaatag taatagttga tttaactgtg aaatggcaat ttaaaggtta tggactttta
    53521 aaaaaatgaa cttaattaca cttaattaaa gataccctat caaatgattt aatcgataaa
    53581 tttatgtcaa taataaaata gaacatgttc taaaattata ttgacaaatt atattacagt
    53641 attacattag tattagtttg ttaaacaagt tttgtgtgga atagtattgt agttaattat
    53701 tttttctgtt tttctgtatg aacaaataca aactattgtt caccatatag gaggtcattc
    53761 aaatggaaga aaaatctgtg atgcaagatc aacgcacaat aacttttaca gagtttatta
    53821 caaagtttat tcctttcacg atacgtatac cgtatatttt gtatcatgga ataaaaggtt
    53881 taaacatgat aagcggaaaa aaaaatatat cgtggggact tcttcttgaa aaaactgcag
    53941 aaaaatatcc caataatata gcaataaagt cacatgaggg aacgcttaca tataaagagt
    54001 tgaatgaaaa ggcaaataaa ttcgctcatt ttttaaaatc aaaaaatatt gttaaaggag
    54061 atactgtcac tttatgtatc gataccagac cggaattact ggtattatat tgtggctgta
    54121 caaaaattgg a
//
LOCUS       DAOOJH010000010        48320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_836_length_48320_cov_10.929183, whole genome shotgun sequence.
ACCESSION   DAOOJH010000010 DAOOJH010000000
VERSION     DAOOJH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 48320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_836_length_48320_cov_10.929183"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLJ75_02665"
     CDS             complement(<1..352)
                     /locus_tag="PLJ75_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04149.1"
                     /translation="MPYSVKIYKHLDELEPKVKRVIMELLEEIDKNVKESVKKEDFND
                     LKNIVARLADAQEKTEQKINQLVESQKALEITMKELAEAQKRTEQRVDALTVKMEELA
                     EAQKRTEQRVDALTV"
     gene            complement(552..1757)
                     /locus_tag="PLJ75_02670"
     CDS             complement(552..1757)
                     /locus_tag="PLJ75_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPD04150.1"
                     /translation="MATGIKDKVAIIGMGCTKFGERWDADANALMVEAYNEAIADAGI
                     EPGEIQAAWVATCFDEINVGKSALAASIALRLPNIAVTRVENFCASGTEAFRGAVYAV
                     ASGAYDIVLAIGCEKLKDIGYGGLPEFSQALGILNGLWFPNVTAPGCFAMLAGGYAAK
                     YGLKMEDVKRAMAHISVKSHANGALNPKAHLQRPVTEEQVLQSPMIAYPLGLFDCCGV
                     SDGAACAIVCSVDVAKAMKKKDFVTVKALQISVSNGEEMAFSNWDGAHVVNTEKAAIR
                     AYAEAGIKNPREELSLLEVHDCFSITELVTMEDLHISERGKAIDDVMDGFFDSTGKIP
                     CQIDGGLKCFGHPIGASGLRMVYEIYLQLHGRADKRQLKNPDIGLTQNLGGFPMMNVI
                     SVAIVGRYT"
     gene            complement(1768..3210)
                     /locus_tag="PLJ75_02675"
     CDS             complement(1768..3210)
                     /locus_tag="PLJ75_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HPD04151.1"
                     /translation="MIGIVSYGGYIPLRRLSRMQVVQQMAWYNSLLYSLMTGERSVAN
                     WDEDAITMAVAAAVDALQNVEQKSIDAVYAASTSFPFDDRQNAAVIAEALNCRNNVRT
                     VDMAGSLKAGVSAVVAALDAVESDSANKVMVTAGERRFTRAASTLELMYGDGAAALVV
                     GRENVIAECIGSYSYHEDFVDHYRGADKRFDYQWEERFIRDEGFGRIIPTAIKLFCDN
                     YHTDINEFACVIYPCPFAREHAGIAKKIKSAHVYTNLHDGVGDTGVAHPLVMLCHALD
                     SAKPGDKILVVGFGQGCEIVAFKVTDAIESFKRPRGITRSIANKKDIPYPKYLKFREL
                     INADTSIRAEAVPNTSLTALWRHRKMLNGFVGYKCKACGTPQFPPYPICVNPACNKSG
                     AMDDYVFSDKMGTVLMYTGDMLAASVEPPALYGLVEFDGGGRTLLDFTDCELEKLNVG
                     TKVAMSFRIHRQDSMRGFTGYFWKAVPIME"
     gene            complement(3327..4229)
                     /locus_tag="PLJ75_02680"
     CDS             complement(3327..4229)
                     /locus_tag="PLJ75_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD04152.1"
                     /translation="MSIKLDGKVAVVTGAGSGIGRTYALEFARRGAMVVVNDLGSLRD
                     GSGANHSAADAVVEEIKKAGGKAVANYDSVATVQGGENIIKTAMDAFGRVDIVVNNAG
                     ILRDKTFVKMTEDEWDIVVSVHLRGAFCVTAPAFKIMREQGWGRIIFTASGAGLYGNF
                     GQTNYASAKMGLVGLMNALKLEGAKYNILVNTIVPVAASRMTEDVLPKEIFEKLKPEF
                     VTPLVMYLCSDECKDTGMLINCGGGWYSRAAIVCASGTIIQGKDEITAEDIMAQWSKI
                     TDLSDAKPLNNIGETFAYMAPLLK"
     gene            complement(4257..5105)
                     /locus_tag="PLJ75_02685"
     CDS             complement(4257..5105)
                     /locus_tag="PLJ75_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPD04153.1"
                     /translation="MPLNETCIGKKYKSDPIKISQHESIYYALAYNEDNDAYFDNRRQ
                     GGIIAPPMYAVFYAAGPTAQLMFDAEIGINMMFVVHYSQYFEWIKAVKPGDEVTSEGT
                     ITHITVKEKGSILGWETVTKNQHDDIVVRAQWEFFDRSAGSGKPDGKAEEGKIPSQFI
                     FDDAMRVRNGQTYIYAEPSGDHNPIHVDDDFAKKVGLPGIILQGLCTMAFAHKMAVDH
                     LTPDRDPLKVKELYVRFARPVLPGQVLTFKGYTMEKLAEKTKYGIVALNDSGKDVLRN
                     GWCTVA"
     gene            5428..6798
                     /locus_tag="PLJ75_02690"
     CDS             5428..6798
                     /locus_tag="PLJ75_02690"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPD04154.1"
                     /translation="MPHNDVKRYYNHDQGIQTLKVSKIITPLTAVVIALFLYQDIYIL
                     HFYFTFWYRFIPIMLCIVYFLLTLSLLKRHVHYVIPLYTLLLLSGIIMILGIAYRIFT
                     NPLYPETFLSNVSTGIQTVFIVLFLIASGARRFLPIVYTVSLVLFLISLSFNKQLTSL
                     ELSALSNPIATTLFLSLLAYVFESMNSREFALRFTVEQNEDELNQYKDTMEKELAFAS
                     YLHRLIMPLKPPVPSISVYYHPYHNLGGDFYDFIPLDKKKDRIGIFISDVTGHGVSSA
                     FITATLKHYLVVGKKYHENPQKLMYYLNEYLTGNIGQNFVTALYCIIDFNKKELIYSN
                     AAHYAPIIVGKDGNIHELEKTGKPFPLGIIKKRKLIKYSKNFSYSTIQLQQGSKLVLY
                     TDGIIELYNAGNPSNFDTGVFFQLLQQYHLLSSADMLNEIDRHLTSQHGNNYINDDIC
                     VIVVDI"
     gene            complement(6801..7259)
                     /locus_tag="PLJ75_02695"
     CDS             complement(6801..7259)
                     /locus_tag="PLJ75_02695"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPD04155.1"
                     /translation="MNKKISILFLCTGNSCRSQMAEGFANALRGDIIEAYSAGIEPKT
                     VDPLAIKVMKEAGVDISHHVSKHVNELINNQFDFVITVCGHAYENCPVFPGKTKVMHR
                     GFEDPPQLAGEAKTQEEALYHYRKVRDQIKAFILELPEWLDKEKTEFIMS"
     gene            complement(7434..8477)
                     /gene="arsB"
                     /locus_tag="PLJ75_02700"
     CDS             complement(7434..8477)
                     /gene="arsB"
                     /locus_tag="PLJ75_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPD04156.1"
                     /translation="MADGVAKKLSFVDRFLTLWIFFAIGAGVAIGYFLPEIKTFIYSL
                     QSGTTNIPIAIGLILMMYPPLAKVKYEEMGKIFQNKRLLGISLLLNWVVGPIVMFGLA
                     VILLRGYPEYMVGVILVGLARCIAMVIVWNELAGGDRELAVGLVAFNAVFQVLFYAVY
                     IYLFITLALNWFGLAEGLDISISISESAKTVFIYLGIPFLAGVATRFGLISYMGKQWY
                     EAKFIPRISPLTLIALLFTIIVMFSMQGNKIIERPQDVVIAALPMLLYFVLMWFVTFY
                     IIKKITGDYSKTAAVAFTAGSNDFELAIAVAIAIFGINSGQAFATVIGPLVEVPVLIG
                     LVDVALVFKKRYF"
     gene            complement(8546..8779)
                     /locus_tag="PLJ75_02705"
     CDS             complement(8546..8779)
                     /locus_tag="PLJ75_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPD04157.1"
                     /translation="MKKFEILGTGCSKCTTLQHLTEQAAKELGIPYQLNKVTDIKQIM
                     SYGVMITPALVVDGQVKIAGKVPSIEEIKKLIQ"
     gene            complement(8825..10123)
                     /locus_tag="PLJ75_02710"
     CDS             complement(8825..10123)
                     /locus_tag="PLJ75_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPD04158.1"
                     /translation="MDWKKELKICMYMGIAFVIFFHLPAGNPRFDNAVSEALKLTRWY
                     AREHVLLCLVPAFFIAGAIATFVSQNSVMKYLGPAAPKLMAYGVASVSGTILAVCSCT
                     VLPLFAGIYTMGAGLGPATAFLYSGPAINVLAIILTARVLGLEIGIARAVGAIVFSIF
                     IGGLMYLIFRKEEIQKANAMMNMPQPETSRPLWQTVIYFLLMVAILVFVNWGKPDAPE
                     GIWYYVYSYKWEITGVVSLLFGLVLWAWFTVTWWKIVIAIIVTVMSGFLFPHRPLIPF
                     VAGIIALIAIGITEKGELLTWLEQTWSFAKQILPLLLGGVLAAGFLLGSPGHEGIIPS
                     QWIAMAVGGNSFFANFFSSVVGAFMYFATLTEVPILQGLLGNGMGHGPALALLLAGPT
                     LSLPSMLVIRSVIGTQKTAVYVSLVIVMATITGILFGLLW"
     gene            complement(10146..10493)
                     /locus_tag="PLJ75_02715"
     CDS             complement(10146..10493)
                     /locus_tag="PLJ75_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD04159.1"
                     /translation="MDTMTQKKYEARAAVMKALAHPTRLYMVEELQKGAQCVCKLQEL
                     IGADMSTVSKHLSVLKSAGLVYDKKEGTTVYYFLKMPCVSEFISCIDTVIQRNATEQL
                     EVARCCINSTQQL"
     gene            complement(11356..11490)
                     /locus_tag="PLJ75_02720"
     CDS             complement(11356..11490)
                     /locus_tag="PLJ75_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04160.1"
                     /translation="MAESKVYALTTMSQPSKKDSSIESIMEATRMRYRRGVVRMYLNS
                     "
     gene            complement(12140..12213)
                     /locus_tag="PLJ75_02725"
     tRNA            complement(12140..12213)
                     /locus_tag="PLJ75_02725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12177..12179),aa:Arg,seq:tct)
     gene            complement(12257..12331)
                     /locus_tag="PLJ75_02730"
     tRNA            complement(12257..12331)
                     /locus_tag="PLJ75_02730"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12294..12296),aa:Pro,seq:tgg)
     gene            complement(12410..13216)
                     /locus_tag="PLJ75_02735"
     CDS             complement(12410..13216)
                     /locus_tag="PLJ75_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HPD04161.1"
                     /translation="MGKPFFDKPARIFAHRGNSREYPENTALAFEKAIELGADVIETD
                     AHITKDGYVVIAHDEELSNITDGNGKIGELPLLQIKQLDAGYNFTQDGETYPFRGKGI
                     TIMTLEEMLETFPHQRFNVDLKDNNILLAEKFCDVIEKHNAQHRVVGASQHGKNLAYI
                     RKRLPGMATSFGLWGVLGFYFLFRSGLLGLFKQFSGDALQIPEFIGPSHLANGTLVRM
                     AHERGIQVDVWTVNSERDMRRLLDIEVDGIITDDIELLKKVLQDYPVEGE"
     gene            13434..13586
                     /locus_tag="PLJ75_02740"
     CDS             13434..13586
                     /locus_tag="PLJ75_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04162.1"
                     /translation="MIQNDMPIPRRYPLIQLRTAQAASPQNDDNNPFSSLTGGNEPAV
                     GNASIK"
     gene            13813..14142
                     /locus_tag="PLJ75_02745"
     CDS             13813..14142
                     /locus_tag="PLJ75_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04163.1"
                     /translation="MKYGKTIFIIIAILSTIFIIGEIGTYIYVEAPLSTDFYSSIPKK
                     DVYKLQNHYGIKVVTGNNWIHLGWIANPDEEYYTIELKNVHGTVKKVGTTQFGSFLFH
                     GKGATSG"
     gene            14220..>14774
                     /locus_tag="PLJ75_02750"
     CDS             14220..>14774
                     /locus_tag="PLJ75_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04164.1"
                     /translation="MCVPFITDNYRTLFIPEHSGYYINDHTIFQDAYGNWQLIGITSK
                     TDGNPDAEKYFAVASSKTFPPNDIMKEESPVADVGELAWAPHVIKHKNIYHMFWSPHK
                     LHHMTSTNGIRWGNHDVIMEKPYHKFFRDCMILQVAKDQWLLYTTARGTFFSQIDVYQ
                     SFNLTQWQYIRTALQSSWGSERNSP"
     assembly_gap    14775..14874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14875..15085
                     /locus_tag="PLJ75_02755"
     CDS             <14875..15085
                     /locus_tag="PLJ75_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04165.1"
                     /translation="QYIRTALQSSWGSERNSPALITTLKAHAPEWIYVPQTKQWYITT
                     CGWKWVATLTNGEAAIAPVSWKCKK"
     gene            complement(15094..18018)
                     /locus_tag="PLJ75_02760"
     CDS             complement(15094..18018)
                     /locus_tag="PLJ75_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04166.1"
                     /translation="MKKIIRYVCYGILLVVFVYACGYMVIPKLLTIGKHSNHNVYVSL
                     GFHVNMYHSYRIDTNDEAGFGKDIRVIRKIIEVLDEKNKKGIPVKGVWDFENLFTLEE
                     ILPQYAPDIIANIKRRVKEHGDEIILMSYNNGLVSAMTQDECMYSVKYAISNSRRSGV
                     KDIFKDYSPFVRPQEMMATSGNYELYKQLGLQGIVLYYSGIPFDAFRVFVDELSLEEA
                     HNPLMYHNKNTHEKMMVIPAYNQADLIENVSIYNWVHMLRYQQLRGTINNDVMICINA
                     DADDSYWYGYDLPSYLKWLPNTGGLSQLIDDIASLDFVTFTTIGDYVKNHKPVKEISF
                     GQDTADGSFNGYVSWAEKKYCSDYWTAVEKDRNIHKLAEAYYSFIGQSAPGDITTALK
                     RSFEKRMRLLSTTNFGMATPFLAKNREHVVETITHSMESLTKPVVADIKKGAKGIIAK
                     QAINTNDGYEYVTSLLLLQQDTAPFVFKLPMELPQGYTYYMQSKDMLTPVNMFSSNGV
                     STIFIGRQPLHSGVYHIVRTQPGTKHYAAEATDTMIKNNTITVTVDNGFIQGVEAHGS
                     TILNKMSLIPWIAYNGQDYRPEKITASVLSDGKNGVAAVALRGEIALPENVTRGYFDY
                     TIYLVDDIDMLFVEGTITYPDTPRTTIFKPGMPALARIYDERWQQVIPCPLFAAMTAT
                     KDIPFTVIKKNYFGVEDTYKIDYFKHSKENLRLANVNNHITAGYVAVTNSKEFIAVAK
                     DNEVLSNFAFCPLKIDYGMVKGFTVSLNPFGTFFGRQYFQPTWGNGQGFKSAILNGDQ
                     YHSGACTYSGTTQRFSLAIAHGRGALQQSVKAQLQSFAHQPFIVAMNEVTPVQQKQKL
                     QSPKGVVALYNNGGVYINFEKVDGAAGYNIYCGNKDALKKIGVTSNTSFFAKNNWDGK
                     PFVEGNTYVAAVSAYDDNGNVNKVPVTYTFVAKPVKESLDLPLTLQLKILYHCLVAQL
                     RW"
     gene            18470..19396
                     /locus_tag="PLJ75_02765"
     CDS             18470..19396
                     /locus_tag="PLJ75_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HPD04167.1"
                     /translation="MKKQLTVMTLFHVLIFFFSISCSSKTENHVTPIVQNILYTKTAI
                     EDYLKNRINQQSSPILFASFWDFDGTIIKGDCSEGLQEENKQVYKGLVQVAIENNFSK
                     KYKNNEYNKFLQHYAYLETHKGHSDAYIYLATLFTGRDIRDLQQLSQHYFSHTLRYYF
                     FASSIAIMNQLKAHGIKIYIISASPDYFVKGAAYSLTVSADHIYGIKIKTNNGIATEQ
                     VIPPLTYAEGKTKLLKSIIDRLKKENKTNHVYAIAAFGNSYHTDGNFLNYVATQTLPA
                     GKPVSVMINGGSPPKKYHALFECVIQNDVIAK"
     gene            19401..20339
                     /locus_tag="PLJ75_02770"
     CDS             19401..20339
                     /locus_tag="PLJ75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPD04168.1"
                     /translation="MKRYAIFFFIILFGLLPLCTPAQNQWGYYCKDTYSNNSKHYWDN
                     TLKSLSVLCFTGITISKNACNIPSFSNEHYLFTKAQQYNIAIIPHVTFTSVTEGMSFL
                     KAREWDNVITTLANALHTNAWAGIHFDFEYLPAAYSNAYAQFLKKFRSIAPDIKLSAA
                     LFPQIEFHKEHAAFHNYEKLSPFLNEVVIMCYDYHNPKTKPGPVTDMQWTMKNIEYAL
                     RFFKAHQIWLGMPAYGYMWKNGKYHSVITTRAIPKYTSIYSNYRHSSGTLCIEYIKND
                     DMFIALVSDTVTIKKLQHLATVYSLKGTALWRLGFE"
     gene            complement(20390..21499)
                     /locus_tag="PLJ75_02775"
     CDS             complement(20390..21499)
                     /locus_tag="PLJ75_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPD04169.1"
                     /translation="MKFLKGFVYMAMMTFATAAFAQEAVKEEIKDIKISGKAYLEWNK
                     ELSNYDDTTEHQNTFEIKRVYLDFKKEFDSVWSARVTTDVGQVESEDEIEVTDTQSGN
                     PETVDVKAKSDPYTVYVKYAYIQAKQKMDFVDANFKFGMIGTPLIGLIDDKSDYRWVE
                     QNYVDQAKNIIGTSIDSSADMGVSVDLSFFKMVKLSGSLVNGEGYKKVNEDELGDDGK
                     AYYGMLTITPVKGLDIFGTYRNQITDDGHGSDNYSRYMAFGASYSTDLVKVGAMYVMP
                     EVKKGGVENEYTLVDSWLLANLESVINIPILITGRYSYGENEDVDDSEVTIWAAGLGY
                     AINKNIRVMAYYQDADNDAKEDPETTFYVKTEVVF"
     gene            21919..22830
                     /locus_tag="PLJ75_02780"
     CDS             21919..22830
                     /locus_tag="PLJ75_02780"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010882079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPD04170.1"
                     /translation="MKPNNLTAEQYSIFKENGTEPPFNNAYWNKKEHGIYVDIVSGEV
                     LFSSLDKFDSGTGWPSFTKPVNNNAVITCSDPSHGMIRTEVRSKSSDGHLGHVFADGP
                     APAGLRYCINSASLRFIPVKKIIEEGYNDYIYLFENTPIGFEQIIIGGGCFWGVEAYF
                     KKVKGILDTTVGYSGGTIKNPDYTMVCTGTTGHAETVLITFNPDIVNLERILKHFFSI
                     HNPSTLNRQGPDIGTQYRSALYYINDNQKITIDTIIQQITSRGAIVTTEVAKAINFFK
                     AEEYHQDYLDKNPGGYCHINLNKIYQV"
     gene            complement(22833..23495)
                     /locus_tag="PLJ75_02785"
     CDS             complement(22833..23495)
                     /locus_tag="PLJ75_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPD04171.1"
                     /translation="MSSFSSYDIPKTDNNDVFQRFTEYFHKPSASHVDSLSDKLRFFR
                     QHNNLSYEQLAAITGICVELLQKIEERRAYPSLRVIHQLTRSLQQAKVIAPGTPVEWG
                     YTVIKKDDVIQLKNERPKNIFLNNVEPSHTADEADVNEVVAVKAYIITLEGNYTKKDF
                     SIHNGEEFIHVKKGSIKITLGSNEVILNHGDSIYFLSLIPHKIENVASSESVIHVIVY
                     QE"
     gene            23787..24641
                     /locus_tag="PLJ75_02790"
     CDS             23787..24641
                     /locus_tag="PLJ75_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04172.1"
                     /translation="MERIAKSIIPHYDLHKIMGFSESMPIPNKDAASQIVHDFSESKY
                     FFKLVCALMEAQEYGIMGRRYPIAHLHEIVTEIINLGFIFDKNNHIFIENANTRKTQH
                     WGVLEENEEYIITLLRMDIAANTQLVRKYTPPLIAHTYQNIQTIVTSAVEQRNGRIWN
                     WEGDGCIAAFILGNKNMTAVFAAIEILHELFLFNELVSKLPEAVNARIAIDTGYCAYK
                     HSSEELKKTELVKKIMEIESHYTPINAITISNTIYASLHPDIAKLFILTRKSATPYYT
                     HTVKWEDI"
     gene            24638..25600
                     /locus_tag="PLJ75_02795"
     CDS             24638..25600
                     /locus_tag="PLJ75_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04173.1"
                     /translation="MNITVLYHKKNASSLQKTFGTLMKSKKYSIEFIDRDEYNKPKKT
                     TANSLYYLDITGLQQADIQKYIKYFNKESMFYGIIDYKNTFSDIAWFFHNNACDYITK
                     ETCNNPISESRIRAIVTFKSITLSETPQKKYIIARDSWKSIKPEQEYTFCFLLIEILD
                     VHELKKHIASEHLDSKIFSFRHFVENTLNQLDGKLWMWMDATGLFIFPFDGKSLPAVD
                     SAIRLTVNRSIISLEHTLFNQIIHYRIAMHIGNTVYQPHGKTGTIISDCINSLFHLGQ
                     KYTADEGLFITEDVYNVLNSRTKGMFIQTHLFEGRQIYRCKNII"
     gene            complement(25606..25911)
                     /locus_tag="PLJ75_02800"
     CDS             complement(25606..25911)
                     /locus_tag="PLJ75_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HPD04174.1"
                     /translation="MNNIHRRKVKYLKACAHGLDPVVLIGKNGLSKAVLNAIDEVLLC
                     HELIKIKFIDCKEERKELARQIVEKTGSNYITMIGNVLIIYRQHPEADKRQYELKAI"
     gene            26382..27152
                     /locus_tag="PLJ75_02805"
     CDS             26382..27152
                     /locus_tag="PLJ75_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014244941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD04175.1"
                     /translation="MSNDTILTKDPGFRYHEKAPKIDAKEFQKVIDSRRSVRIYEKEP
                     IPDKIIQQCLRNALLAPNSSNLQPWEIYHIATPEVRQQMNKACLEQIAAKSAAALFVA
                     VARTNTWKEHAKEMVQLFEAAGNVPKSAIAYYKKLVPFVYNQGLFGIWGLIKRILFFI
                     RGLKTPVIHEPVSHCHMKIWAVKSTALACQNLMLSLRAYGYDSCPMEGYDSKLIKKIL
                     QLPEDAVVVMVISAGKRAIGGIYGPRIRYDENRFLKRV"
     gene            27159..27968
                     /gene="zupT"
                     /locus_tag="PLJ75_02810"
     CDS             27159..27968
                     /gene="zupT"
                     /locus_tag="PLJ75_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HPD04176.1"
                     /translation="MLHNPNVVFAFILTLLAGLATGIGSAISIISKKTNTKVLSLALG
                     FSAGVMIYVSMMEILSKSFDVLYHEYGKSGGWFALISFFGGIALTGIIDIIVPSAENP
                     HEIKRVETMPLPATNREQQLMRLGLLSALAIGIHNFPEGLATFVAALHDTKVGIPIAI
                     AIALHNIPEGIAVAVPIFHATKSRRKAFVYSFISGLAEPVGALIGFLILLPYMSDTIF
                     GIVFGCIAGIMVFISVDELLPSAIKYGEHHYSILGFISGMFIMSVSLILLQ"
     gene            28061..28834
                     /locus_tag="PLJ75_02815"
     CDS             28061..28834
                     /locus_tag="PLJ75_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPD04177.1"
                     /translation="MNSNSFHEIVDDIVIITLNRPKVRNAIDRDTADELSNAFQQFDA
                     NKNLKVAILTGAGGNFCSGADLLAIAEDGHRKNRLNPNIKDNGPLGPTRMLLSKPVIA
                     AISGYAVAGGLELACWCDIRMADMTAKFGVFCRRFGVPLIDGGTQRLPRLIGLSRALD
                     LIITGREINAEEAYQFGLVNYLVTDKDIKQAALELAHKIASFPQQCMRSDRMAVYKGF
                     NMPLSEALEIEFARGTDVIRNGETLEGAKLFAQQKGNKI"
     gene            complement(28890..29420)
                     /locus_tag="PLJ75_02820"
     CDS             complement(28890..29420)
                     /locus_tag="PLJ75_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04178.1"
                     /translation="MKGYNSIIIVLLVYALFSGCATQQKKVGCIEGKCDEGYGVYVWE
                     DGTMYAGNFKSGKFHGQGTYTWANGMTYTGIYVDGKRHGYGVMTWPDGSSYRGEFYND
                     EITGKGKYIYADGKIYTGPMVKGKANGIGILEWPDGTRYEGEFVDNRIEGKGTLIYPD
                     GRTLKGRFINGILVNE"
     gene            complement(29474..29743)
                     /locus_tag="PLJ75_02825"
     CDS             complement(29474..29743)
                     /locus_tag="PLJ75_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD04179.1"
                     /translation="MKITVEKIAEDDLKKQGVLSWPIWQKEISDFDWFYDTTEQFYVL
                     EGEVEVDTVDGQKVRFGAGDFVTCPKGIQCTWHVKKPIRKHYNFK"
     gene            complement(29998..30669)
                     /locus_tag="PLJ75_02830"
     CDS             complement(29998..30669)
                     /locus_tag="PLJ75_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04180.1"
                     /translation="MPSARFTITPEVKRQIVKIIDDRINEVHVTKEDFSELKSIVKDI
                     AKAQKELTQAQKKTEQRVAELAEAQKELADAQKRTEIEVKSLVAGLHDTRVELGGLSR
                     SFSYAFENEAYRHLPAVLKEKYGYTIKERIVRAEIGGKEINFFARAMKDGKEVFIVGE
                     SKIRLESTWRKDVFSELEDKVNAVKAEFGDVEVVRLLVTHFAKKDFLDEAEKMGVIVV
                     QSYQW"
     gene            30995..31306
                     /gene="trxA"
                     /locus_tag="PLJ75_02835"
     CDS             30995..31306
                     /gene="trxA"
                     /locus_tag="PLJ75_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007329897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPD04181.1"
                     /translation="MALIQEVTDATFDSSVLQTSGKVLVDFWAPWCVPCRLQTPILER
                     LVSNGINAKIVKCNTDVNHGTAQKFGISAIPTLILFDNGKEIDRMVGVQPEEVLKRRL
                     Q"
     gene            complement(31415..32185)
                     /locus_tag="PLJ75_02840"
     CDS             complement(31415..32185)
                     /locus_tag="PLJ75_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04182.1"
                     /translation="MELLRLHNVSLWRNSTIILRNINLTVNTGDQWAVLGPNGSGKSF
                     LMNIIATLVFPSEGQVVIAGETLGQVNVWDVRKHIGIVSDYLQTHYPQETMVAEVVAS
                     GFFSSMGLYQELSPAMEQKALTILHSLELVHYAQTPFGKLSYGEQKRVLIGRALVYDP
                     DILILDEPCNGLDMKAREEFLQQLTMLIKTGKHIIYVTHHVDEIMPWINRVLLLNNGQ
                     CMYAGTKNILLNEDVISKVMGCSVGIFQHNSRIFSYVK"
     gene            complement(32237..33235)
                     /locus_tag="PLJ75_02845"
     CDS             complement(32237..33235)
                     /locus_tag="PLJ75_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPD04183.1"
                     /translation="MRYKSIILVLFLILTACSKKEAVDTIVTINSFFGDVTITSHEGN
                     KPPAVGQVLTISDTIVTGANSVVDLLYRNSGIIRVNENTTVVVSSLIKAGSDDVVLTL
                     EKGKTFATLGKLKKGDGFAIKAKTVIAAVRGTSFRLVADEKASRVDVVSGKVMVKPVN
                     NETVIEDIEVIVEENQTVTLDTKTAATISQKIDENKVEVVAEQEQQKIIDSIKETIKP
                     TEIPKETVKEIKQEVKDIPVITTVQEEIKQEIQKVIETDKEAKQRKNEEKLKKAEEAK
                     ALKLKLQNEQEAKILAEKLAKEKAEQEKVEKEKAEQEKAAKEKKIKEDRIKNIPTL"
     gene            33683..34978
                     /locus_tag="PLJ75_02850"
     CDS             33683..34978
                     /locus_tag="PLJ75_02850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPD04184.1"
                     /translation="MDRITQNDTLIDTLGNCTIDSPLTIPKFISDSQRIAVDIEADII
                     EQSLEKGKIPSFENAGPRKKIFFKPQNTRAAIVTCGGLCPGINNVIQGIVRMLTFQYG
                     VKTIFGVKYGFEGLVPRFGHSFIELTPQFVHDLHEKGGTVLGSSRGRQDEKEIVDTLV
                     AHDINILFVIGGDGSLRGAYDIYREVKKRNLPIAIVGIPKTIDNDIWFVDKTFGFSTA
                     VSTAAQAIYAAHAEAIGAKNGIGIVRVMGRDAGYIAAYVTLASNEVNYCFIPEVHFDL
                     EGAHGFLPHLEKRLYAASHAVIVVAEGAGQEYFDEPKLTDVSGNIKYGDIGLLLNNTI
                     GAYCKQRNIPVTIKYIDTSYLVRSTPASSDDAVYCIMLSQNAVHGAMAGKTGFVVGSW
                     HSYYTYIPLSLVTKKRKRIDPHGYLWSIVKQSTCQPDFF"
     gene            35265..36257
                     /locus_tag="PLJ75_02855"
     CDS             35265..36257
                     /locus_tag="PLJ75_02855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPD04185.1"
                     /translation="MTANDMDQLFKIKQLYANIGKLITSSLEIEHILEGIMEEVHTFF
                     GPDNWSLLRYDPNSEELYFVISHGIDFDSIKNIRLQKGEGVAGYVVETKKPVFIPNTS
                     HDTRFSDRIDRITGFSTKSVMAVPLIFRDTMYGVIELINKSTGGIFSSDDYLILQTIA
                     DFGAIAFANSLLYEKALTLTALDSLTGLYNNKKLNDIIASWSKDEAHIRRDDTTHVIA
                     VLNIDIKGLNEINTIAGHRDGDRVIKHISRILKTSLRENDMTFRTGGGNFISIINCDN
                     YFDMKSLVERILHKISAITVPDYPDLHIQVSHGLSIGTRNRINELIQETLEQKS"
     gene            complement(36307..36777)
                     /gene="tadA"
                     /locus_tag="PLJ75_02860"
     CDS             complement(36307..36777)
                     /gene="tadA"
                     /locus_tag="PLJ75_02860"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPD04186.1"
                     /translation="MNNNDNNPEFYMTIALEEAKKAHDEGEIPVGAAIVFKDGVIATA
                     HNTTRQCSDPTAHAELIAIKQAAMVLKNERLNGCRMYVTKEPCAMCAGAIVHARIEKL
                     YIGVKDKRFGACGTVLNVCGNGVLNHKPQIIFGILEEECKNLLKSFFEQLRKKG"
     gene            37032..38903
                     /locus_tag="PLJ75_02865"
     CDS             37032..38903
                     /locus_tag="PLJ75_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD04187.1"
                     /translation="MMRNPLVDHRDVYFVLFEVLETDKLQQFPKFAEHDKDMYEATID
                     LALKMATEEVYPVNKEADKIGVRYDPDTKKVIIPQGYKGGLRKYNEAGFVGMIEDPEI
                     GGMGMPGAIAIAVNEIFTSASLSFTTYPGLSHGAAALIENFGTDEQKKVYLEKMLSGQ
                     WGGTMCLTEPEAGSDVGNLKTKAIPQPDGTYRIQGQKIFITSGDNDFYENIIHPVLAR
                     IEGDPPGTKGISIFIVPKYRINPDGSIGELNDVVCSGIEHKMGIHGSATCSLNFGDNG
                     TCAGYLLGQPRKGMKIMFHMMNEARLGVACQGLALSSTAYMHAVTYARNRIQGVHVTQ
                     MLNPDAQKVAIIEHPDVKRMLLWMKGYVEGMRMLNYYLAHNATLIEVLDGEAKKEAQA
                     IVEILIPIAKAGCTDSAVLITSEAIQVYGGYGYISDYPVEQYMRDAKITQIYEGTNGI
                     QSMDLTMRKILMNPEQYNYTIMKKRMSEAIANAKGIVEEKYINLVENGMQKMDEVILM
                     LKEQMAAGKFLHIFAACTPVQQAMFMLTLAWLHLWAMTLTIPKMKALVGEKKGEERDA
                     FLSENEEASYYSGKVLSSQFYLGAEFPKFFGKIDAILFNETAIIKASKDIFTGALLE"
     gene            39129..39611
                     /locus_tag="PLJ75_02870"
     CDS             39129..39611
                     /locus_tag="PLJ75_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04188.1"
                     /translation="MDFETTTCISVEHRALLSQYCEKLDIPLRTFIVYCIMYAAKHEK
                     KKEVAFKRISYRKRNTDNPWKRVHLELYQSEYEFFLDVKKLWKMSLANIIAYCVDNVL
                     FEFFEYFSKQIEKIKTDNYLSYYENRSYSFNFYRVKGIHCLKFYWGPPPEMLGQPLTQ
                     "
     gene            complement(39742..40401)
                     /locus_tag="PLJ75_02875"
     CDS             complement(39742..40401)
                     /locus_tag="PLJ75_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD04189.1"
                     /translation="MQITEHVYQIGGSTLSHPSDAAIYCVTDGKVTALIDAGTGDGHD
                     RVLKNIAKENIDASTVHYLFLTHCHFDHVGGAHKLRGTFNCKVVAHQLDAQYIETGDQ
                     YVTAANWYGSFLTPCPIDIKVNDSKKSFAIGSLTLNMYHIPGHSPGSVALTVESEGKL
                     VLFGQDVHGPLNDVLKSNRDDYIQSLEFLLSLDADILCEGHFGVYCGREEVKNFIESF
                     L"
     gene            complement(40527..41885)
                     /gene="thrC"
                     /locus_tag="PLJ75_02880"
     CDS             complement(40527..41885)
                     /gene="thrC"
                     /locus_tag="PLJ75_02880"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007330874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPD04190.1"
                     /translation="MGGDSNYRAVFRCIAGCSEEYPLDEVVYRCKKCGGLLEVYHDRD
                     RLRELSPKKWKELFNSRVGTTKWPYGSGVWSKKEWVCPEVDNENIVSMFEGNTNLFWA
                     KRFGAMIGMHDLWIKMCGNSHTGSFKDLGMTVLVSMVNEMIMRGKPIKAVACASTGDT
                     SAALAAYCAYAGIPSIVFLPRNKISTAQLVQPMSNGSIVMSLDTDFDGCMRIVQEITK
                     DNTIYLANSMNSLRIEGQKSISVEIVQQFDWEVPDVIIIPGGNLGNVSALGNGFDMML
                     QLGLITKKPRIVLAQAQNANPLYRAYRNGFKEFKPITPQKTLASAIQIGDPVSVTKAI
                     KALQQFDGIVEQASEDELSDAAALADRTGMYSCPHTGVALAVLLKLVEKQVISKTDKT
                     VVISTAHGLKFSEFKVGYHQGTLPDVKSIYANRPIELEANADRVREVLFNELQRRSTV
                     AY"
     gene            complement(41888..43165)
                     /locus_tag="PLJ75_02885"
     CDS             complement(41888..43165)
                     /locus_tag="PLJ75_02885"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPD04191.1"
                     /translation="MKVRCGLIGFGTVGSGVYHLLEKHRGDILKKTGIDIELVKICDV
                     RNIASMVAIPVTDKWEDIIYDTSIDTVIELIGGTEPAKSIIVSALKAGKNVVTANKKL
                     LAENGDEIIAASMSVKGSLAFEAAIGGGIPCVAALRNGLVGNSIQSVVGILNGTTNYI
                     LTRMSEDDMSFAEALALAQDKGYAEADPTFDIEGYDAAHKIVLLSMLAYNVGVSMKSV
                     YVEGITNITALDITYAHEMGYTIKLLGVSRMLDCKLDIRLQPVMIPLTNPLASVRNEF
                     NAIMYHGDMTGPVTLYGKGAGSFPTASAVVSDIVSIAQKQNGGKDCIFIEKDAEYISP
                     EYRMLRYYIRLYSEDRAGILSKIAGVLGGYNISIASVIQKEAMTEYVPIVITTHRANE
                     AGMRKAKNEIERFNFVNGHVMIIPIEDFDNTGE"
     gene            43464..44195
                     /locus_tag="PLJ75_02890"
     CDS             43464..44195
                     /locus_tag="PLJ75_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04192.1"
                     /translation="MGEKITIINLHKSFGQQVVFSGLHLSVHEGEILCIIGKSGSGKS
                     VLLKHCIGILKPDSGSILVDGQKISNDDSDASIIKKFGVLFQGGALFDSMNVYDNIAF
                     PLRRQGASEEIIAQKIPELLAHVGLKGIEARMPSELSGGIQKRVALARAIAKNPEIML
                     YDEPTTGVDPINSGSLYRLIAYMRKIFHVTSVIVTHDMRLAFTIADRIAMLHDGTIIV
                     EGTPQQIKECQDAVVQQFVSGKAKV"
     gene            complement(44197..44586)
                     /locus_tag="PLJ75_02895"
     CDS             complement(44197..44586)
                     /locus_tag="PLJ75_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPD04193.1"
                     /translation="MIEVKVTTYSIGELAKLLEMSTRTIRYYEELGLLNSVKRIDNGR
                     RIYTDDDVRRLKLIKRLKILGLTLSEMHELEYIWQIHKTNDIVLKRLLEILDLHIRRI
                     DDRMKDLEILKNEIGEYKSRIKNKING"
     gene            complement(44599..46251)
                     /locus_tag="PLJ75_02900"
     CDS             complement(44599..46251)
                     /locus_tag="PLJ75_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD04194.1"
                     /translation="MAFKRLTIGKALYDTVEKYPDHEALVCPEFGVRLTYKQFYHKCR
                     EVARGLIALGVKKGDHVSVWATNVPEWVYLQFSLGMIGGVLVTVNTSYKSHELEYQLK
                     QSDSTTLFMIEGYRDSNYHQIVRELIPNLDCAQPGMINEPKLPMLKNIVYIGKRDATP
                     GMFTFHDMVMMGNAISDAALDKRISECDVDDVINMQYTSGTTGFPKGVMLTHYNIVNN
                     GLMVGDVMGTSPHDKLLIHVPLFHCFGCVMSTLNCVTHGAAMVVMESFDPLKSLQAVA
                     SEKCTAINGVPTMFIAMLNHPDFDSYDMSSLRTGIMAGAPCPVETMNQVRTRMHCPDI
                     VIAFGQTESSPVMTMTRRDDPVSLRVSTIGRLLPDIEGKIVDPETGEDLPPNIQGEIV
                     TRSACVMKGYYKMPEATANAIDKDGWLHTGDLGSVDENGYFKVTGRIKDMIIRGGENI
                     YPREIEEFLLTNQNIQDVQVVGIPDEKYGEQVLAVIQLKEGQSSSAEEMVAFCTGRIA
                     RHKIPKYWEFVKAFPMTASGKVQKYKLREMFASKYGMAASVF"
     gene            46725..47147
                     /locus_tag="PLJ75_02905"
     CDS             46725..47147
                     /locus_tag="PLJ75_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPD04195.1"
                     /translation="MDYTILTAPCGLDCFNCPMYLAANDGSLRIKIAKSMNISDEQAV
                     CKGCRNENGTIGFLNMKKQCTVYACTTSKGIHFCHECDDFPCDNLHPYADRASQVPHN
                     TKVFNLCLIKKMGLEKWAQEKAKDVKKIYFGGKFTLTE"
     gene            complement(47203..47982)
                     /locus_tag="PLJ75_02910"
     CDS             complement(47203..47982)
                     /locus_tag="PLJ75_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD04196.1"
                     /translation="MQLNNFKEEITTVWSFPERGKWATHNSRYRGNYAPQVARNLIQR
                     YTGQYDWILDPMVGSGTSLIEAKLLCRNIIGIDINPYAIELSKKALSFEVEDGNCKQE
                     AYVGDARKLSNISDESIDFIIFHPPYLNIIKYSQGVIKEDLSNISNVGKFFDEIEIIA
                     KELLRVLKHDKYCAVLIGDTRKGQHYIPLSYFLLQRFLKLGFILKEEIIKTQHNCVYS
                     KRWEENAKRFGFYLIMHEHLFVFRKPKLGENLLKIRWSCHI"
     gene            complement(47984..>48320)
                     /locus_tag="PLJ75_02915"
     CDS             complement(47984..>48320)
                     /locus_tag="PLJ75_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04197.1"
                     /translation="FECEAEKHIFRWHSSRNRTCQICKKSRATIIKKVLPCTDNEGYI
                     AIQKNVFVTGKDAIFPDVNECPFVVVCKPRSENFKKLNPPKSISILGQTGWESARTKA
                     EEGGGGLMS"
BASE COUNT        15092 a        10182 c         8111 g        14835 t
ORIGIN      
        1 ttactgttaa tgcatcaaca cgctgctccg ttcgcttctg tgcctccgcc aattcctcca
       61 tctttactgt taatgcatca acacgctgct ccgttcgctt ctgtgcctcc gccaattctt
      121 tcatagtaat ctcaagtgct ttttgagatt ctactaactg attaattttt tgttccgttt
      181 tttcctgggc atcagctaaa cgagcaacaa tattcttcaa atcattaaaa tcttcctttt
      241 ttactgattc cttaacattt ttatcaatct cttcaagaag ttccatgatt acacgtttca
      301 ctttgggttc aagctcatcc agatgtttat aaatctttac actatacggc atcctgatac
      361 ctctttttac tgaatatttt tatatatgct atgcaagatt ttgttaccca ttaactatct
      421 attccaattc aaaaatagta agattatgta ttttcatcaa taattttatt aatttttttt
      481 aaaaggtatc attaatctta taactttaat gatacctttt tattgacaat atatatctat
      541 tgttcagtgg ctcatgtata tctaccaaca atcgcaacac tgatgacatt catcattggg
      601 aatccaccta aattttgcgt taatccaata tcaggatttt taagctgccg cttatcagcc
      661 ctgccatgta attgcaggta tatctcgtat accatccgta agcccgatgc accaataggg
      721 tgcccaaagc atttgagccc tccatcaatc tgacagggaa ttttaccggt actatcaaaa
      781 aatccatcca tcacatcatc aatagctttt cctcgttcag atatatgtaa atcctccatg
      841 gttacaagct cagtgatgga aaaacagtca tgaacctcta aaaggctcaa ctcctcacga
      901 ggatttttta tgcccgcttc cgcataagca cgaattgcag ccttttcagt attgacaaca
      961 tgtgcgccgt cccaattgga aaatgccatc tcttcgccat tggataccga aatctgtaac
     1021 gcttttactg tcacaaaatc cttcttcttc attgcctttg caacatcaac actgcatact
     1081 atcgcgcagg ctgctccatc gctaaccccg caacagtcaa atagccccag aggataggca
     1141 atcattggcg attgcagtac ctgctcttcc gttaccggac gctgcagatg agcttttgga
     1201 ttcagagctc cattagcatg acttttaacc gagatgtgag ccattgccct tttaacatct
     1261 tccatcttta aaccatactt tgctgcataa ccgcctgcca gcatagcaaa gcaacctggt
     1321 gccgtaacat tgggaaacca tagtccatta agaattccca gagcctggct aaattcgggc
     1381 aagccgccat agccaatatc tttgagcttt tcacagccaa ttgccagcac aatatcatac
     1441 gcaccggagg caacagcata cactgcacca cggaatgctt cggtaccgct tgcacaaaaa
     1501 ttttcaaccc gtgtaaccgc aatgttgggc aatcgcagcg caattgaagc ggctaatgca
     1561 cttttaccaa cattgatctc atcaaaacag gttgcaaccc atgctgcctg tatctctccg
     1621 ggttcaatac ctgcatcagc aattgcttca ttgtacgctt caaccataag ggcgtttgca
     1681 tccgcatccc agcgctcgcc aaattttgtg caccccattc caattattgc aactttatct
     1741 tttattccag tagccatata ttcctcctta ctccattatt ggtaccgctt tccagaaata
     1801 tccggtaaag cctcgcatgc tgtcctgtct atggatacga aacgacatag ctacttttgt
     1861 acccacatta agtttctcga gctcacagtc tgtaaaatca agaagcgttc ggccaccacc
     1921 atcaaactca acaagcccat acagtgccgg tggttcaaca gatgctgcaa gcatatcacc
     1981 agtatacata agcactgtgc ccattttatc cgaaaataca taatcatcca tggcacctga
     2041 tttgttacat gcaggattaa cacaaatagg ataaggagga aactgcggtg taccacatgc
     2101 cttacattta taccccacaa aaccattcag catctttcgg tgacgccata atgcagtaag
     2161 ggatgtattg ggaacagctt cagctcgtat acttgtatcc gcgttaatta attccctaaa
     2221 ctttaaatat tttggatacg gaatatcttt cttatttgca atgctccggg taatccctct
     2281 gggacgctta aaactttcaa ttgcatcagt taccttaaag gcaactatct cacaaccctg
     2341 tccaaaacca acaacaagta ttttatcgcc aggttttgca ctatcaagcg catggcaaag
     2401 catcaccagc ggatgtgcca cacctgtatc accaacccca tcgtgcaggt tagtataaac
     2461 atgtgccgat ttaatttttt ttgcaatacc tgcatgttcc cgcgcaaatg gacagggata
     2521 aataacacag gcaaactcat ttatatctgt gtgatagtta tcgcaaaaca gctttattgc
     2581 ggtagggatg atacgcccaa atccctcatc gcgtataaaa cgttcctccc attgataatc
     2641 aaagcgttta tcggcaccgc ggtaatgatc aacaaaatct tcatgatacg aatatgaacc
     2701 aatacattct gctataacat tttcacgtcc cactaccagg gctgcagcgc catcaccata
     2761 cataagctcc aatgttgatg ctgctctggt aaaccgtctt tcaccagcag taaccataac
     2821 cttgttagcc gaatcagatt ctacagcatc cagcgcagca acaactgccg aaacgcctgc
     2881 ttttaatgaa ccagccatat caacggttcg cacattgttc ctgcaattga gtgcttctgc
     2941 aataactgcg gcattctgtc tgtcgtcaaa tggaaacgat gttgaagctg cataaacagc
     3001 atcaatactt ttttgttcaa cattttgcag tgcatcaact gctgctgcca cagccatagt
     3061 aattgcatct tcgtcccagt ttgcaactga ccgttcacca gtcatcagtg agtatagcaa
     3121 agaattatac catgccatct gctgtacaac ctgcatcctg gataatcttc ttaaagggat
     3181 gtatccccca tacgaaacaa tcccaatcat tgaaaaagcc ccccattaaa atatttttta
     3241 tacattatcc tgaccccctg cattgttttt attatggtag atttatagtt tcagggttta
     3301 gaaccatgtg agcaaaattt tttcacttac ttaagtaatg gtgccatgta tgcaaaggtt
     3361 tctccaatgt tattcaatgg ttttgcatca cttaaatcgg ttatcttact ccactgcgcc
     3421 atgatatcct ctgctgttat ttcatcttta ccctgtataa tagtgccact ggcacaaaca
     3481 attgcagcac ggctatacca accgccacca cagttaataa gcatccctgt atccttacat
     3541 tcatctgaac atagatacat caccagcggt gttacaaatt ccggttttaa tttttcaaaa
     3601 atctctttag gcaatacatc ttctgtcatg cgggacgctg ccaccggaac tatcgtgtta
     3661 accaatatat tgtactttgc accttctaat ttcaatgcat tcatcaatcc aacaagtccc
     3721 atctttgctg atgcatagtt tgtttgccca aaattgccgt ataaaccggc tcctgaagca
     3781 gtaaagatga ttctgcccca accctgctca cgcattatct taaacgccgg tgcagtaaca
     3841 caaaatgctc cacgcagatg caccgatact acaatatccc attcatcttc ggtcatcttg
     3901 acaaacgttt tatcacgtaa aatgccagca ttgtttacta caatgtccac ccgtccaaaa
     3961 gcatccattg cagttttaat aatgttttca ccaccctgca cagttgcaac agaatcataa
     4021 ttagctactg ccttgccccc agcttttttt atttcctcca caacagcatc agcagcacta
     4081 tggtttgcac ctgatccatc acgcaaactg cctaaatcat tgacaaccac cattgcacca
     4141 cgtcgtgcaa actctaaggc ataggttcgc ccaatccctg aacctgcacc cgtaacaaca
     4201 gcaactttgc catcaagctt aatactcata ctaccctcct gctcattaat aatatttcag
     4261 gcaacagtac accatccatt gcgcagtaca tccttgccac tatcatttaa cgctacgatt
     4321 ccatattttg ttttttctgc aagcttttcc attgtgtacc ctttaaacgt aagaacttgg
     4381 ccgggcaata ccggtcgggc aaaacgcaca tacaattcct taacttttaa aggatctcta
     4441 tccggtgtaa gatgatccac tgccatctta tgagcaaatg ccatggtaca tagcccctgc
     4501 aaaataatcc ccggcaaccc aacctttttt gcaaagtcat catcaacatg gatagggtta
     4561 tggtctccgg aaggttcagc atagatatag gtctggccat tccttaccct catggcatcg
     4621 tcaaaaataa attgtgaagg tattttaccc tcttcagcct ttccatcagg ttttccactt
     4681 cctgcacttc tgtcaaaaaa ttcccactgt gcccttacta caatgtcatc atgctgattt
     4741 ttagtaactg tctcccagcc taaaatgctt cctttttctt ttaccgtaat atgggtgata
     4801 gttccctcgg aggtaacctc atcgccgggc ttaaccgctt ttatccattc aaaatattga
     4861 ctgtaatgta caacaaacat catgtttatc ccaatctcag catcaaacat tagttgtgcg
     4921 gttggaccag cggcataaaa aacagcatac atcggaggag ctataatacc accctgcctt
     4981 ctgttatcaa agtatgcatc attgtcctca ttataagcaa gggcataata gatactttca
     5041 tgctgtgaaa ttttaatagg atctgattta tattttttgc caatacacgt ctcattcagc
     5101 ggcatacgca cctcctgttt ttatatttaa gtgaagtaga tttttcccac agtcccaatt
     5161 aaatgtaata caaacataca tgttttttgg tgtcaagata ttaatctgac aaaaaatagt
     5221 tatattaatt tagctatata gttatacatt acttgttttg gcaagaatta atgatatatt
     5281 gttaagaaat gatcataata aatacgtatt gacttaacca actttaatta tcataccatg
     5341 attgaatgca atggtggtaa aaaatttttt tgataatggc tggtgagtta aaaataattt
     5401 ttacaggtat ctattaatgt taactatatg ccgcataatg atgtaaaacg atattataac
     5461 catgatcaag gtatacaaac acttaaggta agcaagatta ttactccact tactgctgtt
     5521 gttattgcgc tgtttcttta tcaggatatt tatattctgc atttttattt taccttctgg
     5581 tacagattca ttccaataat gctatgtata gtctactttt tgctaacgct ttctttacta
     5641 aaaaggcatg tacactatgt tattccgcta tataccttac ttttattatc agggattata
     5701 atgatactgg gtattgcata tcgcatattt accaaccctc tctatcctga aacattttta
     5761 tcaaatgtat ctacaggcat tcaaacagta tttatagtgc tatttttaat agcatcaggt
     5821 gcaagaagat ttttaccaat agtatacact gtttctttag tgcttttcct gatatctcta
     5881 tcattcaata aacaattaac atctttggag ttatcagcac tttccaatcc tattgccaca
     5941 actctttttt taagtttatt agcttacgta tttgaaagta tgaattccag agaatttgca
     6001 ttgcggttta ctgtagaaca aaatgaggat gaactcaatc aatataaaga taccatggaa
     6061 aaagaattag ccttcgcatc atacctgcat agattaatca tgccgcttaa acctccggta
     6121 ccatctattt cagtatacta tcatccatat cataatttag gcggtgattt ttatgacttt
     6181 ataccacttg ataaaaagaa agacaggatt ggcatattta tcagtgatgt aacagggcat
     6241 ggggtatctt ctgcatttat tactgcgaca ctaaaacact atttagtagt tggcaaaaaa
     6301 tatcatgaaa atccacaaaa attgatgtac tatcttaatg aatatttaac agggaatatt
     6361 ggccaaaatt ttgtaacagc attatattgt atcattgact ttaataaaaa agaattaata
     6421 tacagcaatg cagctcatta tgcaccaata atagttggga aagatggcaa catacatgaa
     6481 ctggaaaaaa caggaaaacc tttcccatta gggattataa aaaaaagaaa attaataaaa
     6541 tatagcaaaa atttttctta ttcaacaata cagttgcagc aaggttcaaa attagtatta
     6601 tataccgatg gtattattga actatataat gcagggaatc catccaattt tgatacaggt
     6661 gtattctttc aattattaca gcaataccac ctgctatcat ctgccgatat gttaaacgaa
     6721 attgacaggc atcttaccag tcagcatggg aacaattata ttaacgatga tatatgtgta
     6781 atagttgttg atatataata tcagctcatt ataaattctg ttttttcttt atcaagccat
     6841 tcgggcaatt ccagtataaa tgctttgatc tgatcgcgta cttttctgta atgatacaat
     6901 gcttcctcct gtgtttttgc ttcccctgcc agttgaggtg gatcttcaaa tcctctgtgc
     6961 atcacttttg ttttaccagg aaacaccgga caattttcgt atgcgtgccc acaaaccgtt
     7021 ataacaaaat caaactgatt attgattaac tcattaacat gctttgacac atgatgtgaa
     7081 atatcaacac ctgcttcttt cataactttt attgccaaag ggtcaactgt tttgggttca
     7141 atccctgccg aatatgcttc aatgatatca ccgcgtaatg cattggcaaa accttctgcc
     7201 atctgactac ggcaggagtt tccggtacac agaaataaaa tggaaatttt tttattcata
     7261 acaacctttt taaaaaagat ttttatatca tggcatatca ccattgttat cacaactaca
     7321 gaaacagtag tataaatttt ttgttaattt ttgttataca atatttttat atatacttac
     7381 taccagtttt gtaaatctcg taaaagttca aagtaaaaca cgcaaattct taattaaaaa
     7441 tatcgtttct taaacaccag ggcaacatct acaagtccaa ttaataccgg aacctcaacc
     7501 agaggaccaa taacagttgc aaatgcctgc cctgagttaa ttccaaaaat tgcaatagca
     7561 acagctatag ctaactcaaa atcatttgaa ccagcggtaa aagctactgc tgcagttttt
     7621 gaataatctc ccgtaatttt tttgataata taaaatgtaa caaaccacat caaaacaaaa
     7681 tataacagca ttggtaatgc tgcaatgaca acatcctgtg gacgttcaat gattttattc
     7741 ccttgcatag aaaacattac aataattgta aaaagtaatg caatcagtgt tagcggactt
     7801 atcctgggta taaattttgc ttcataccac tgtttaccca tataagatat taaaccaaaa
     7861 cgcgtagcaa cacctgctaa aaacggtatt cctaaataga taaaaactgt tttagcagat
     7921 tcagatattg atatagaaat atcaagtcct tcagcaagac caaaccaatt caaagccagt
     7981 gttataaata aatagatata cactgcataa aacaatacct gaaatacagc attaaatgca
     8041 actaaaccaa ctgccaattc cctgtcacca cctgctaatt cattccatac tattaccatg
     8101 gcaatacatc tagcaagccc tacaagaata actccaacca tatattccgg atatcccctt
     8161 aaaagaataa cggccaggcc aaacataaca attggaccaa caacccagtt cagaagcaat
     8221 gatattccaa gcaatctctt attctggaat atttttccca tttcttcata ttttactttt
     8281 gcaagtggtg gatacatcat aagtatcaaa ccaattgcga taggaatgtt ggtagtaccc
     8341 gactgaagtg aatatataaa tgttttaatt tctggtaaaa aatagcctat tgctacccct
     8401 gccccaattg caaaaaaaat ccacagagtt aaaaatctgt caacaaacga aagcttttta
     8461 gctaccccat cagccataaa ttcacctctt tacttatatt ctgcaagccc ccttgtaggt
     8521 acaaccatta aaaaaataaa tctatttatt ggattaactt tttaatctct tcaatagagg
     8581 ggactttgcc agcaatctta acctgaccat caacaacaag agctggcgtt atcatcacac
     8641 catagctcat tatttgttta atatcggtta ccttattcaa ttggtacggt atacctaatt
     8701 cctttgctgc ctgctcggtt aaatgctgaa gtgtggtaca ctttgaacag cctgttccta
     8761 aaatttcaaa ttttttcata accacctcaa aaaatagact atatatgggg tcagagcctc
     8821 aatactacca tagcaatcca aacaatattc cggtgatagt tgccatcaca atgaccagtg
     8881 aaacatatac agcagtcttt tgtgtaccaa taacgctacg aataaccagc atactgggaa
     8941 gggacaatgt aggtccagcc agaagaagtg ctaacgccgg accatgaccc ataccattac
     9001 ctaaaagccc ctgtaaaatg ggaacttcgg taagcgttgc aaaatacata aaggcaccaa
     9061 caactgatga gaagaaattg gcaaagaatg aattaccacc cactgccata gcaatccatt
     9121 gtgatggtat aataccttca tgccccggac tgccaagtaa aaagcctgca gccagaaccc
     9181 cacccaaaag caatggcagt atttgtttgg caaatgacca tgtttgctcc aaccatgtca
     9241 acaactcacc tttttcggtt atgccgatag ctatcaaagc aataatacct gcaacaaatg
     9301 gtattaatgg cctgtgcgga aataaaaagc ctgacattac tgtaacaatg atagcaataa
     9361 ctatcttcca ccatgttaca gtaaaccatg cccacagcac caatccaaag agtagtgaca
     9421 caacaccggt aatctcccat ttataactgt acacataata ccagattccc tcaggtgcat
     9481 ctggtttacc ccagttaaca aacaccagta tggctaccat taaaagaaaa tagataaccg
     9541 tctgccacag gggacgtgac gtttcgggct ggggcatatt catcatagca ttggcttttt
     9601 gtatctcttc ttttcggaaa ataagataca tcaatccacc aatgaaaata ctgaaaacaa
     9661 ttgcaccgac tgcgcgtgca atgcctattt caagtccaag cacccgtgct gtcaaaatga
     9721 ttgccagtac attgatggca ggtcccgaat acaaaaaagc tgttgcagga cctaatcctg
     9781 cgcccatagt ataaataccc gcaaaaagcg ggaggaccgt gcatgaacaa acagctaaaa
     9841 ttgtccccga aacagatgcg acaccatacg ccataagttt tggtgcggca ggccccaggt
     9901 acttcatgac cgaattttga ctaacaaatg tggcgatagc tcctgcaatg aaaaacgctg
     9961 gtacaaggca taacaagaca tgttcgcgag cataccacct tgtcaacttc agcgcctcag
    10021 atacagcatt gtcaaaacgg ggattgccag caggcaaatg aaagaaaatg acaaaggcta
    10081 tacccatata catacatatt tttagttctt ttttccagtc catggccaat ccttatacct
    10141 ggtaattata actgttgcgt actgtttata cagcaacgag caacctctaa ctgttctgtt
    10201 gcattccgtt gaatcaccgt atcaatacag ctaataaatt cactaacaca gggcatcttc
    10261 aaaaaataat aaacggttgt gccctctttt ttatcatata ccagccctgc acttttcaat
    10321 accgataggt gtttggatac tgtggacata tcagcgccaa tcaattcctg cagtttgcat
    10381 acacactgtg cccctttttg cagctcttct accatatata accgcgtggg atgtgccagc
    10441 gccttcataa cagcagctcg tgcctcatat tttttttgtg tcatggtatc cataataacc
    10501 tcgtattatt tcttatttgg caatttagca aaatagtcaa taattatcaa aaaaaattac
    10561 agtaataaaa tatcaaccaa tcctaaatat tacatggtat gttgaagttc tgcaaataaa
    10621 tatgttgtac actacttttt acatgtcact aacaagccgc attgaagtaa atagttttta
    10681 caactgtatg ctgaaaagaa tgatagtggc acgccgatta aaatattgac aattgatagt
    10741 tttaaaatgt aaatagtaat tgttaattac atgcataacg atgcacacaa aaaagttctt
    10801 gaatatggca tgttaaagta taataaatgt aaaccgtaat tatgatcttc aacaatagtt
    10861 tacatactat aaccatggag tgtaattaca caataataca cgatagcagg tgcatatgga
    10921 taaaaatact atacgtacag cttcacaatt agcttttacc ataaccaata ccattatcaa
    10981 acactttgga gcgcgtcctt catgctctga acagtcaaac aatgctgcaa aagtactgca
    11041 gtttatgtat tcaatacatt gtgatagctc tcaacttgaa acattcacta cccatcccag
    11101 tgcgttttta ggcttcctta ccctggtgcc atggatttat atagcctgcc ttatttttat
    11161 tattttacac tgttatatca ttgctgcagt tgggtttacc atttccatta tcattgctac
    11221 atcacaattc attttttata aaggaacatt tgatttttta tatacacaaa aagagggcac
    11281 taacgctatt ggtagtattg aaccgtcaaa cgatgtacgc cagcagatta taatcagcgg
    11341 acatcatgac agtgcctatg aattcagata catgcgcacc acaccgcgcc tgtaccgcat
    11401 acgtgttgct tccattatac tttcaatact actatccttt ttgcttggct ggctcatcgt
    11461 tgtcaatgcg tacacctttg attccgccac tgcacatact gttctcctaa cctttcttat
    11521 aattctgggc attgctaata tgcagatgct tttttttaaa agcaaacacg caactcccgg
    11581 agccggagac aacttaatcg caagtaccat tactgtagca ctggcaaaca tttttgcaaa
    11641 ctatcgcccc aactataccc gcataatttt tgcaagcttt gatgctgagg aaagcgggct
    11701 caagggctca aaagcatttg ccaccagtca caaacatgaa attggcagca tgccaacatt
    11761 tcatttcaac atggacagcc tctataaggt tgatttaata caatttttaa caagcgatgt
    11821 aaacggcttc gttcctcttt caaaagaact tgcaaggcaa tgtgtggcga tagctcatgc
    11881 tatgggctac cctgccaaaa catttgccat gtaccccgga acaggtgcaa ccgatgcagc
    11941 acctgttgca caagctggcg caatagcaac aacgctcatt gcactcccaa cggaggttga
    12001 aaaacaacac agcgtgtatc acaccatgga cgacaccacc agcaatatac agcctgaagt
    12061 tgttgaagca gcaatagcaa ttgctgtgaa gcttgttcac tatattgaca atgaaatacg
    12121 cggatacagg tggggaaatc gcgccagaca ggactcgaac ctgtggccca cggcttagaa
    12181 ggccgttgct ctatccagct gagctactgg cgcacatagt agtacattat atattcttag
    12241 actatatttt aaaaattcgg ggcgagagga tttgaacctc cgacctcatg gtcccaaacc
    12301 acgcgctcta gccggactga gccacgcccc ggaatgacag taaaaaaaat tatatcgcac
    12361 acaacgattt tgtcaaagta ataaatagta tagttgtgtc aatatttttt tattcccctt
    12421 ctacgggata atcctgcaaa acttttttta acagttcaat gtcatccgta atgataccat
    12481 caacctctat atccagcagc cgtcgcatat cacgttcgct attcactgtc cacacatcaa
    12541 cctgtatgcc cctttcatgc gccatgcgta ccagtgtacc atttgccaga tgtgaaggtc
    12601 caataaactc cggaatttgc aacgcatcgc cggaaaactg tttaaataat cctaacaatc
    12661 ctgaacggaa taaaaaataa aaaccaagaa caccccatag cccaaatgat gttgccatac
    12721 caggtaaacg cttgcgaata tatgcaaggt tttttccatg ctgcgaagcc cctaccaccc
    12781 tgtgttgagc attgtgcttt tcaatcacat cacaaaattt ttcagccagc agaatgttgt
    12841 tatctttcag gtcaacatta aaccgctgat gaggaaatgt ctccagcatc tcctcaagcg
    12901 tcattatggt tatacctttc cccctgaatg gataagtttc cccatcctgt gtaaaattgt
    12961 atccagcatc aagctgttta atctgcaaca ggggaagctc accaatcttt ccgttaccat
    13021 ctgttatgtt cgatagttcc tcatcatgag ctatcaccac ataaccatcc tttgttatgt
    13081 gagcatctgt ttcaatgaca tcagctccca gctctattgc tttttcaaac gccaatgctg
    13141 tgttttcggg atattccctt gaatttcccc tgtgagcaaa gatacgagca ggtttatcaa
    13201 aaaaaggttt tcccataatt gttaaccctc aatttttgcg actttgcatg ttatccatga
    13261 ccttgatgct gataccttca gccagaatac actgtttgat actcctcaga aaatagtatt
    13321 atttaaccat gtcattccgg gcccggtccg aaatctcttt taccactacc gtcattccga
    13381 acattgtccg gaatccgaat aattaacgtt agattctgaa accagttcgg gatatgattc
    13441 aaaatgacat gcccatccca cggcgatacc ccttaattca acttcgcacg gcacaggcgg
    13501 cttcgcctca gaatgacgac aacaatccat tttcatccct tacaggtgga aatgaaccag
    13561 ccgtcggcaa cgcctcaata aaataatact acaattaata ctacaatccc tgttattcat
    13621 atttactgta tctttaaaaa ccctgtcaac atttttactt accagacagt agcaaaaaaa
    13681 catacagtat tttacttgat gaaaataaag taatatgtat aataaactca tattaatttg
    13741 aattttcaaa caaatagatg aagcatttat gaattgacat acatagaaca tggaggtaca
    13801 ggggagtatt gaatgaaata tggcaaaaca atctttatta ttatagcaat actatcaacc
    13861 atttttataa ttggtgaaat tggtacttac atctatgtag aagctcccct gtctacagat
    13921 ttttattcat caatcccaaa aaaggatgtg tataagcttc aaaaccacta tggcattaag
    13981 gttgttaccg gtaacaactg gattcactta gggtggattg ctaatcccga tgaagaatac
    14041 tatacaatag aacttaagaa tgttcatggc actgttaaaa aagttggaac gacacaattt
    14101 ggttcatttt tatttcatgg taaaggagca acttccgggt aataaggata aataaaaaaa
    14161 caaagcatca tatagagctt ggcaacattc aggtttttgc ttccagcacc aaggatggta
    14221 tgtgtgttcc atttattacc gataactatc gcacactatt cattccagaa cattccgggt
    14281 attatataaa tgatcatact atatttcagg atgcatacgg caattggcag cttattggaa
    14341 ttacctcaaa aactgacggc aatcctgatg cagaaaaata ttttgccgtt gcttcaagta
    14401 aaacatttcc acccaatgat ataatgaagg aagaatcacc ggttgctgat gttggcgaat
    14461 tagcatgggc accgcatgtt ataaaacaca aaaacatcta ccatatgttc tggtcaccac
    14521 acaaacttca tcatatgacc tcaaccaatg gcatacgatg gggtaatcat gacgtaatta
    14581 tggaaaagcc gtatcataaa ttcttcagag attgcatgat actacaggtg gctaaagacc
    14641 agtggttgct atacaccact gcccgtggta catttttttc tcagattgat gtatatcaat
    14701 cattcaacct aacccaatgg caatatataa ggactgcgct gcaaagtagt tggggtagtg
    14761 agcgcaattc accannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngcaata
    14881 tataaggact gcgctgcaaa gtagttgggg tagtgagcgc aattcaccag cattgattac
    14941 aacattaaaa gcgcatgcac ccgaatggat ttatgttcca caaacaaaac aatggtacat
    15001 aacaacctgc ggatggaaat gggttgcaac gcttacaaac ggtgaagctg caatagcacc
    15061 ggtgtcatgg aaatgtaaaa aataaaacac actctaccag cgtagctgtg caacaaggca
    15121 gtgatataaa atcttaagtt gcagtgttaa cggcaaatca agactttctt ttacaggctt
    15181 tgccacaaat gtatatgtta ctggaacttt gtttacattg ccgttatcat cataagcgct
    15241 aactgctgca acataggtat tcccttcaac aaatggtttg ccatcccaat tattttttgc
    15301 aaagaacgaa gtattgctgg ttacaccaat tttttttaat gcatccttgt tgccacaata
    15361 aatattgtat cctgctgcgc catcaacctt ttcaaagttt atatatactc cgccattatt
    15421 atataacgcc accactcctt ttggtgactg cagcttttgt ttttgctgaa ccggtgtcac
    15481 ttcattcatg gctacaataa atggctgatg agcaaatgat tgcagttgag cctttacact
    15541 ttgctgcaaa gcccccctgc catgagctat cgccaaacta aaccgttgtg ttgttccact
    15601 ataggtacat gcccctgaat ggtattgatc cccgttaaga attgctgatt taaatccctg
    15661 tccattaccc catgttggct ggaagtattg cctgccaaaa aaagtaccaa acggattaag
    15721 gctaactgta aaacctttaa ccatcccata atcaatcttc agcggacaaa aagcaaaatt
    15781 tgaaagcacc tcattatcct ttgccactgc aataaattct ttactgttgg ttactgcaac
    15841 atagccggcg gtaatgtgat tgttcacatt ggcaagccgt aaattttctt ttgaatgttt
    15901 aaaataatca attttgtaag tatcctcaac accaaaataa tttttcttga tgacagtaaa
    15961 cggtatatct tttgtggctg tcatcgctgc aaacagcgga cagggtataa cctgctgcca
    16021 ccgctcatca tagatgcgag caagcgccgg catacccggt ttaaagatag tggtgcgggg
    16081 agtatccgga taggttattg taccctcaac aaacagcatg tcaatgtcat caacaagata
    16141 tatggtatag tcaaaatatc cccttgttac attttcaggc agagctatct cgccacgcag
    16201 tgccactgca gccaccccat ttttgccatc actcaatact gaagcggtaa tcttttccgg
    16261 gcgatagtcc tggccattgt aggcaatcca tgggattaaa ctcattttgt taagtatggt
    16321 actgccatga gcctcaacgc cctgaataaa tccattgtca accgttaccg ttatggtatt
    16381 attttttatc atggtatcgg tagcttcagc agcatagtgt tttgtacccg gttgtgtgcg
    16441 cacaatatga tagacaccgc tatggagagg ttgcctgcca ataaagattg tagacacacc
    16501 attgctggaa aacatattaa caggggtaag catatctttt gactgcatgt aataggtata
    16561 tccctgcggt aactccattg gcagtttaaa aacaaaggga gcagtatcct gctgtaacag
    16621 cagcagtgat gtaacatact cataaccatc gtttgtgttt attgcctgtt ttgctataat
    16681 cccttttgca ccttttttta tatcagccac caccggtttt gttaatgatt ccatggaatg
    16741 ggtgattgtt tcaacgacgt gctctctgtt ttttgccaaa aatggtgttg ccataccaaa
    16801 gtttgttgtt gaaagcaatc gcatgcgttt ttcaaacgat ctttttaatg ctgtcgttat
    16861 atctccgggt gctgattgtc caataaatga ataatatgcc tcagccaatt tatgaatatt
    16921 tctgtccttt tcaacagcag tccagtaatc actgcaatat tttttctctg cccatgaaac
    16981 atacccatta aagcttccat cggccgtatc ctgcccaaag ctgatttctt tcaccggttt
    17041 atgatttttt acataatcac ctattgtcgt aaacgtaaca aaatccagag atgcaatatc
    17101 gtcaataagc tgtgacaggc cgcccgtatt agggagccat tttagatatg acggcaggtc
    17161 atatccatac cagtacgaat catcagcatc ggcattgata catatcataa cgtcattatt
    17221 tattgttcct ctcaactgct gataccgtaa catatgcacc caattgtata tgctaacatt
    17281 ttcaattaag tcagcctgat tgtatgcggg tatcaccatc attttttcat gagtattttt
    17341 gttatgatac ataagcggat tatgagcttc ttcaagcgat agttcatcta caaaaactct
    17401 aaatgcatca aatgggatgc cactatagta taaaacaatt ccctgcaagc ccagctgttt
    17461 atagagctca taattcccgg atgtagccat catctcctga gggcgaacaa aaggtgagta
    17521 gtccttaaat atatctttga cgccacttct tctggaattt gaaatagcat atttgactga
    17581 atacatacat tcatcctgtg tcattgctga taccagacca ttattatacg acatcaggat
    17641 tatttcatca ccatgttcct tcacacggcg ttttatgttt gcaataatat cgggagcata
    17701 ctgaggcaaa atttcttcca gtgtaaaaag gttttcaaaa tcccatacac cttttaccgg
    17761 aatccctttt ttattttttt catccagcac ttctatgatc tttcgtataa cgcgtatatc
    17821 tttcccaaat cccgcctcat cattggtatc tatcctgtat gaatgataca tgttcacatg
    17881 aaatcccagc gaaacataga cgttatgatt ggagtgtttg ccaatagtca aaagttttgg
    17941 aataaccata taaccacagg catagacgaa gacaaccaga agtatgccat aacaaacata
    18001 ccgaataatc tttttcatac tgtcctccac aataaaaatg atagcatctg aaccaacgct
    18061 aacatcgcta accttcaatg cagaaagtta agtcattccc attatttatg attttttcca
    18121 tttattcatc acaatacctg atgaataccg ttacgctcat acctgccatt catacatcat
    18181 aaggattatt gtaacatcat gctataaaaa tacaactaaa atagtgaaag tattaatgta
    18241 cactaacccg tttaatttta tattgatttt aatgtttaaa cacactacaa tattccggga
    18301 cagtgtaaaa tggtcatttc acaatggcaa aattatatac catgcatcag gtgtaatcct
    18361 attatgggca taaactattg gtgtaagggg ctctcatcca ttatttactg tacaatacaa
    18421 acctttttca atgtgcccat gctataaaac gtttttaaaa ggattaaata tgaaaaaaca
    18481 actaacagta atgacattgt ttcatgttct tatttttttc ttcagcatat cctgcagcag
    18541 caaaactgaa aaccatgtaa cacctattgt gcaaaatatt ttatacacaa aaactgccat
    18601 agaagattat ttgaaaaaca gaataaacca gcaatcgtca cctatactat ttgccagttt
    18661 ctgggatttt gacggcacaa taataaaagg tgattgttct gaaggattac aagaagaaaa
    18721 caaacaagtc tataaaggac tggtacaggt agccatagaa aataattttt caaaaaaata
    18781 taaaaataat gaatacaata aatttttaca acactacgca tatcttgaaa cacacaaggg
    18841 acacagcgat gcatatatat atctggcaac attatttacc gggagggata tccgcgatct
    18901 acagcaatta tcgcaacact atttttcaca tactcttcgt tactattttt ttgcatcatc
    18961 aatagcaata atgaatcaac ttaaagcaca tggaatcaaa atatacatta tttcagcaag
    19021 tcctgactat tttgtaaaag gtgctgcata ctcattaacc gtatcagcag accacatcta
    19081 tggaataaaa attaaaacca ataatggaat agcaacagaa caggtgatac caccacttac
    19141 ctatgctgaa ggaaaaacaa aattgctaaa atcaatcatt gacagattaa aaaaagaaaa
    19201 taaaacaaat catgtgtatg caatagctgc gtttggcaac agctatcata ccgacggtaa
    19261 ttttttaaac tatgttgcaa cgcaaacact ccctgctggt aaaccggtta gcgtgatgat
    19321 caatgggggt agcccgccca aaaaatacca tgcattattt gaatgcgtca tacaaaatga
    19381 tgtaatagca aaataacttt atgaaacggt atgccatatt ttttttcata attttatttg
    19441 gtttattgcc tctttgtaca ccggcacaaa atcaatgggg gtattattgt aaggacacat
    19501 acagcaataa ctccaaacac tactgggata acacactcaa aagcctttca gtactgtgtt
    19561 ttactggaat cactatttct aaaaacgcat gtaacatccc ttcatttagc aatgagcact
    19621 atctttttac taaagcacaa caatataata ttgcaattat acctcatgta acattcacaa
    19681 gtgtaaccga ggggatgtca tttttaaaag cccgagagtg ggataatgtc attacaacac
    19741 ttgcaaatgc attacacaca aatgcatggg caggtattca ttttgacttt gaatatctac
    19801 ctgcagcata cagcaatgct tatgcacagt ttttaaaaaa atttagatcg atagctcccg
    19861 atataaaact ttcagcagca ctttttccac agattgagtt tcataaagaa catgcagcat
    19921 tccacaatta tgaaaaactg tctccattct tgaatgaagt tgtcattatg tgttacgatt
    19981 atcacaatcc taaaacaaag cctggaccgg taactgatat gcaatggacc atgaaaaata
    20041 ttgaatatgc attacggttt tttaaagcac atcagatatg gttaggcatg ccggcttatg
    20101 gatatatgtg gaaaaatggg aaataccatt cagttattac tacacgtgca attccaaaat
    20161 atacatcaat ctacagtaac tatcgccaca gctccggaac actgtgtatt gaatatataa
    20221 aaaatgatga tatgttcatt gccctggtat cggataccgt aactataaaa aaactacagc
    20281 accttgcaac agtatattca ttaaagggaa ctgctttatg gcgtttgggc tttgaataaa
    20341 aaaggggcat ccctgcccct taatcgtgga gtatctggag ggttaacact tagaatacaa
    20401 cttcggtctt aacatagaac gtagtttccg gatcttcctt tgcatcattg tctgcatctt
    20461 gatagtatgc catcacacgg atgtttttgt tgattgcata ccctaatcct gctgcccaga
    20521 tggttacttc agaatcatca acatcttcgt tttcaccata tgaataccgc ccggtgatta
    20581 agattgggat attgataacg ctttccagat tggcaagcaa ccagctatca accaaggtat
    20641 attcattttc aacgcctcct ttcttgacct cgggcataac atacatagcg ccaaccttga
    20701 ccaaatcagt tgaatatgat gcaccaaatg ccatgtagcg gctataattg tccgaaccat
    20761 gaccatcatc agttatttga tttcggtagg ttccaaatat atcaagccct ttcacaggag
    20821 taatggttag cataccgtaa tatgctttcc catcatcgcc caattcatcc tcgtttacct
    20881 tcttgtatcc ttcgccgttt accagagatc ctgacagctt aaccatttta aagaaagaaa
    20941 gatcaacact tacacccata tcggcggaac tgtctattga tgtaccaatg atgtttttag
    21001 cctgatcaac ataattctgc tcaacccatc ggtaatcact cttgtcatca ataagcccaa
    21061 taagtggagt accaatcata ccaaacttaa aatttgcatc aacaaaatcc attttttgtt
    21121 tagcctgtat ataggcatat tttacatata ccgtatacgg atcgcttttt gctttaacat
    21181 ctactgtttc aggatttcct gactgggtgt ctgtaacttc aatttcatct tctgactcta
    21241 cctgcccaac atcggttgta acccgtgcgc tccacacact atcaaattcc tttttaaaat
    21301 caaggtaaac acgcttgatt tcaaatgtgt tctgatgctc ggtagtatca tcgtaattgc
    21361 tcagttcctt attccactct aaatacgctt tccctgaaat tttaatatcc ttaatctctt
    21421 cttttacagc ctcctgagca aacgctgccg tagcaaacgt catcatagcc atatacacaa
    21481 aaccctttaa aaacttcatg taacaacctc cttaactaat agttaattgt attgattttt
    21541 aaaaacaatc tgagggtaag aaagcagtct gatataaaat agatttgaag gatatgttaa
    21601 tatttagtta aatttcattt tttttactgc tgtcgctttt tttattgccg gtaatcgtgc
    21661 tctgtcacag ggcaacggca ttcttccagg cagcaattat gtacgttgaa aaaatcccgg
    21721 ctgtacactt aagtttttaa gggtgaaggc ttattaccga taactatcgc caaaataata
    21781 actacaatat taaaatgatt atattatttt attgactttt ttattgtttt cactatattt
    21841 agataggata ctaatggcca tgaaattgtt tttttagcat ggttttttat ttacagaaaa
    21901 acttcaagag atacgggcat gaaacctaac aatctaacag cggaacaata ctctattttc
    21961 aaagaaaatg gcaccgaacc accatttaac aatgcgtatt ggaacaaaaa agagcatggc
    22021 atttatgttg acatagtatc aggagaagtg ctattttcat cgcttgataa gtttgactca
    22081 ggtaccggct ggccaagttt tacaaaacca gtaaacaaca atgctgttat cacatgctct
    22141 gacccctcac atggaatgat acgcaccgaa gtgcgcagca aaagctcaga tggccattta
    22201 gggcatgtat ttgccgatgg tcctgctcct gcgggattgc ggtattgcat caattctgcc
    22261 tcattgcgat tcatcccggt aaaaaaaatt atagaagaag ggtataacga ttatatctac
    22321 ctctttgaga atacgcctat agggtttgaa cagataataa ttggcggcgg ttgtttctgg
    22381 ggtgttgaag catactttaa gaaagtaaag ggcatacttg ataccacagt tgggtattcg
    22441 ggtggaacaa ttaaaaatcc cgattatacc atggtgtgta cgggtaccac cggtcatgca
    22501 gaaacggtgc tcattacatt taatccagat attgtaaatc ttgaaagaat actaaaacat
    22561 tttttttcaa ttcataatcc ttctacgctc aaccgccagg gccctgatat aggcacacaa
    22621 tatcgttcag cgttatacta tatcaacgat aatcaaaaaa ttactattga taccattatt
    22681 cagcaaatta catccagggg ggcgatagtt actactgaag tagctaaagc tataaatttt
    22741 tttaaagccg aagaatacca tcaagattat ttagataaaa atccaggcgg gtattgtcat
    22801 attaatctga ataagattta tcaggtttaa ggttattcct gataaacaat gacatggatt
    22861 actgattcgc tggatgcaac attttctatt ttgtgtggta tgagcgatag aaaatagata
    22921 ctgtcaccat ggtttaaaat aacttcattt gaaccaagag ttattttaat ggaacctttt
    22981 ttaacatgga taaattcttc accattgtgg atggaaaaat ccttctttgt ataattccct
    23041 tccagtgtaa taatgtatgc tttaacagca actacctcat taacatcagc ttcatcggca
    23101 gtatgcgatg gttcaacatt gttcaaaaaa atatttttag gcctttcatt ttttaactgt
    23161 ataacatcat ccttttttat gaccgtatat ccccattcaa caggggtgcc cggggctatt
    23221 acctttgcct gttgcagtga ccgtgtaagc tgatgaatta ctcgcaagga aggatacgct
    23281 ctgcgttctt ctattttctg taacaactcc acacatatgc cagttatagc ggccagctgt
    23341 tcataactca gattattgtg ttgcctgaaa aagcgtaatt tatcgcttaa cgaatcgaca
    23401 tgagatgctg atggtttatg aaaatattct gtaaagcgct ggaaaacgtc gttgttatct
    23461 gttttgggaa tatcgtatga tgaaaaagag ctcatggaat gttccttaac acaattagta
    23521 tatattcaac tgcaaaacaa cctctcgtta tgatccttac catatgcacc accacacgaa
    23581 catgcatcaa ttccgctgct ataacatcaa cttctgataa ttaatatatc ctcataatta
    23641 tttttatcat ataaaatatt cttttacaac aaaaaatttt tgacaaaatt ttaaatgccg
    23701 tttaatgtat caatattatg aaaattccct tctccatttt atcgataacg gaaaaagcac
    23761 tgacaaaatc catggatgtc cacaccatgg agcgcattgc aaaaagtatt atacctcatt
    23821 acgatctcca caaaatcatg ggcttttcag aaagtatgcc aatccccaat aaagatgccg
    23881 catcccagat tgtgcacgat ttcagcgaat caaaatattt ttttaagtta gtgtgcgccc
    23941 tgatggaggc acaggaatac ggcatcatgg gacgcaggta ccctatagct catctgcacg
    24001 aaatagttac agaaataatc aatttgggtt ttatctttga taaaaataac cacattttta
    24061 ttgaaaacgc aaacaccaga aaaactcaac attggggtgt tttagaagaa aatgaagaat
    24121 acatcatcac gttgctcaga atggacattg cagcaaatac acagcttgta agaaaatata
    24181 caccaccgtt aattgctcat acctatcaaa acattcaaac cattgtaacg tctgctgttg
    24241 agcagcgtaa tggcaggata tggaactggg aaggtgatgg ctgcatagct gcttttattc
    24301 tgggcaataa aaatatgact gccgtttttg ctgctataga gatattacac gaactttttt
    24361 tatttaacga gcttgtatca aaactgcctg aagcagtcaa tgccagaatt gcaattgata
    24421 ccggttattg tgcttacaaa cattcttcgg aagaattaaa aaaaactgaa ctggtaaaaa
    24481 aaatcatgga aatagaatca cactatacgc caatcaatgc aattaccata agcaacacaa
    24541 tatatgcatc attacatccc gatattgcaa aactttttat tttaaccagg aagagcgcca
    24601 caccttatta tactcacaca gtaaaatggg aagatatatg aatatcactg tactctatca
    24661 taaaaaaaat gcatcatcgt tacaaaagac ttttgggaca ttgatgaaat caaaaaaata
    24721 ttctattgaa ttcattgatc gtgatgaata caataaacca aaaaaaacca ctgcaaattc
    24781 attatactat ttagatatta ccgggctaca acaagctgac attcagaaat atataaaata
    24841 ttttaataaa gaatcaatgt tttatggaat aatagattac aaaaatacgt tcagcgatat
    24901 tgcatggttt tttcacaata atgcatgtga ttatataaca aaagaaacct gtaacaatcc
    24961 aatttcagaa agtcgtatac gggcgatagt tacctttaaa tcaattacat tatcagaaac
    25021 accgcaaaaa aaatacatta ttgccagaga ttcatggaaa agcatcaaac cagaacagga
    25081 atacaccttc tgttttcttt taattgaaat tcttgatgtg catgaattga aaaaacatat
    25141 tgcatctgag catctggata gtaaaatttt ttcatttcgt cattttgttg aaaatacact
    25201 gaaccaactg gacggcaaac tatggatgtg gatggatgct acgggtttat ttattttccc
    25261 ctttgatggg aaatcactcc ctgctgtcga tagtgcaatt cgtcttacgg tcaatcgcag
    25321 tattatatcg cttgagcaca cactctttaa tcaaataatt cattacagga tagccatgca
    25381 tattggtaat accgtatatc aaccacatgg caaaacaggt acaataatat cagattgcat
    25441 taattcattg tttcacttag ggcaaaaata cactgctgat gaaggactat tcattactga
    25501 ggatgtatat aatgttctta attcaagaac aaagggcatg tttatacaaa cacatctttt
    25561 tgaagggcga cagatatatc gatgcaaaaa cattatctaa ataagttata tagccttcaa
    25621 ttcatattgg cgcttgtcag cctcaggatg ttggcggtag ataataagca cattaccaat
    25681 catggtaata taattacttc ctgttttttc aacaatctgc cgggctaatt ctttgcgctc
    25741 ttctttgcaa tcgataaact ttatcttaat aagctcatgg cacaataaca cttcatcaat
    25801 tgcattgagt acagccttag aaagaccatt tttacctatc aacacaaccg gatctaagcc
    25861 atgtgcacag gctttaagat atttaacttt tctgcggtga atgttattca tacaaccctc
    25921 aaaataatga aaaaattaaa attgctctat cacacattct tgccgcacat accttatggt
    25981 attttaaaac tgcttgtttt tccaatccat aacttgcaaa aacagtttta tcattttctt
    26041 gtgtccatag aggtattaca atttaaagtt ttgaatatgc cttatttttt agtaattgac
    26101 attacattgt cagtataata acaattaatg tctatacaat taatgatagt attattgcaa
    26161 taattttttt attaattgtt acacggtggt tgcaaatgca aaataattct gcgaagtgag
    26221 ttgacaatgg ttggcatccg gtcatccata cggtatgaaa ataactgacg atagttatcc
    26281 tgaaccctga ttcgggattt tggataatta tttagattac agcaggggcg cattataatg
    26341 cgctaaaata acaaaatcta ttttcaggag gaataataac aatgagtaac gatactatct
    26401 taacaaaaga ccctggtttc cgataccacg aaaaagcacc aaagattgat gctaaagaat
    26461 ttcaaaaagt gattgattcc agacgatcgg ttcgcatcta tgaaaaggaa ccaattcctg
    26521 ataaaataat tcaacaatgc cttcgcaatg cactgctggc accaaattca tcaaaccttc
    26581 agccatggga gatatatcac attgctaccc cagaagtacg gcagcagatg aacaaggcat
    26641 gccttgaaca gatagcagca aaaagcgctg cagcattgtt tgtggcagtt gcacgaacaa
    26701 atacatggaa agagcatgcc aaagagatgg tacaactttt tgaggcagcc gggaatgtgc
    26761 caaaatcagc cattgcctat tataaaaagc tggtaccgtt tgtttacaat caaggattat
    26821 ttggaatatg gggattgata aaaaggatac tctttttcat tcggggatta aagactcctg
    26881 tcatccatga accagtttca cactgccata tgaaaatatg ggcagtgaag tcaacagcgc
    26941 tggcatgtca aaaccttatg ctttcactac gtgcgtatgg atatgattca tgccccatgg
    27001 aaggatatga ttcaaagctc ataaaaaaaa tattacaatt accagaggac gctgttgttg
    27061 tcatggtaat ctctgccggt aagcgtgcaa tcggcgggat atatggtccc cgtatcagat
    27121 atgatgaaaa cagattttta aaaagagtgt aatatactat gctacacaat cccaatgttg
    27181 tatttgcctt cattttaaca ttgttagcag gacttgcaac tggtataggg agtgcaatct
    27241 caatcatcag caaaaaaacc aatacaaagg tactatcgct tgcattggga ttttcggctg
    27301 gtgtaatgat ctatgtttcc atgatggaga tactttcaaa atcatttgat gtattgtacc
    27361 atgaatatgg aaagtcagga ggctggtttg cattaatatc ctttttcggt ggcatagcat
    27421 taacaggaat aattgatatt attgttccat cggcagaaaa tccgcatgag ataaagcgtg
    27481 ttgaaactat gcctctgcca gcaacaaacc gggagcagca gcttatgagg cttgggcttt
    27541 tatcagccct tgctattggc atacacaatt ttccagaagg gctggctaca tttgttgctg
    27601 ctctccatga tacaaaggtt gggattccta ttgcaattgc tattgcgctg cataacatcc
    27661 ctgaaggtat tgctgttgct gtacctatat tccatgcaac caagagcaga agaaaagctt
    27721 ttgtatattc ttttatttcc ggattagcag aacctgttgg agcgttaata ggttttttaa
    27781 tcctattacc ctacatgtcc gacaccatct ttgggattgt atttggatgt attgcaggca
    27841 ttatggtgtt catatcagtg gatgaattat taccatcagc aataaaatat ggtgagcacc
    27901 attattcaat tttaggattt atctctggca tgtttatcat gtcggtatcg cttatactat
    27961 tacaataaat tttatctttg cactacacta ttaatttgac aacaagtagt tttttttgta
    28021 atgtaatagt ttcaaaatta aacaatgaaa ggattagata atgaatagta atagtttcca
    28081 tgagatagtt gatgatattg tgataatcac cctaaacagg ccaaaagttc gtaatgccat
    28141 tgaccgtgat actgccgatg agttgagtaa tgcttttcaa caatttgatg ccaacaaaaa
    28201 tttaaaagtt gcaatcttaa ccggagccgg gggcaatttc tgttccggtg ctgatctact
    28261 ggcaattgct gaggatggac accgtaaaaa caggcttaat ccaaacatca aggataatgg
    28321 gcctcttgga ccaacgcgca tgttgctttc aaagcctgtt attgcagcaa tatcagggta
    28381 tgcagtggca ggggggctgg aattagcctg ctggtgcgat attcgcatgg ctgatatgac
    28441 tgctaaattt ggtgtgtttt gcagacgatt tggcgttcca cttattgacg gaggtactca
    28501 acggttgccc cgcttaattg gattaagcag agcattagat ttaattatta ctggcaggga
    28561 aattaatgca gaagaagcat atcagtttgg attggtcaac tatctcgtta cagataaaga
    28621 tataaaacag gcagcactgg aattggctca caagattgct tccttcccac aacaatgtat
    28681 gcgaagtgac aggatggcag tttataaggg ttttaatatg ccattatctg aagctctgga
    28741 aatagaattt gccagaggaa ctgatgtcat acgcaacggt gaaacattgg aaggtgcaaa
    28801 attatttgca cagcaaaaag gaaataagat ttaactattg atgaatgata aattgccgtt
    28861 gtggtacact aattttttac aaagctacac tattcattaa ctaaaatgcc attaataaaa
    28921 cgtcccttta atgtacggcc atcaggatag ataagagttc ctttgccctc aatcctgtta
    28981 tcgacaaact caccttcata tcgtgtacca tcaggccatt ccaatatacc aataccatta
    29041 gctttaccct ttaccattgg gcctgtgtat atcttaccgt cagcatatat atatttccct
    29101 tttcctgtaa tctcatcgtt gtaaaattct cctctgtaag aactaccatc aggccatgtc
    29161 ataacaccat agccatgcct tttgccatca acataaattc ccgtgtatgt cataccattt
    29221 gcccatgtat aggtaccctg cccatggaac ttgcctgatt taaagttacc agcatacatt
    29281 gtaccatctt cccagacata cacaccatac ccctcatcac acttaccttc aatacatccc
    29341 actttttttt gctgtgttgc acaacctgaa aaaagagcgt atacaagtaa aacaataatt
    29401 atactgttgt agcctttcat tttccaacct ccatcaaata aaaaatactt tccaaaaaat
    29461 atgacaatca acactattta aaattatagt gctttcgtat tggttttttc acatgccatg
    29521 tgcattgtat accttttgga caggtaacaa aatcaccagc accaaatcgc accttttgcc
    29581 catcaacggt atcaacctca acttcaccct caagaacata aaattgctcg gttgtatcat
    29641 aaaaccaatc aaaatcggat atctccttct gccatattgg ccaagacaat acgccttgtt
    29701 ttttaaggtc atcttctgct attttttcaa cagtaatttt catgggcatt cttctccaaa
    29761 ataaaattat gcgtcaatat cattccgggc ttgacctata atcaaaataa ttatcataaa
    29821 tcctgaaacc tgagcatcat atgattcaga atgacacgtc cagcattgtc attctgaggg
    29881 atgcaattcc aaagaatttg tttgtttata aagaagattc ttcgctattg ctcaggatga
    29941 cattgcatta atactaagat catttactgt tacatcgtat cttgctatca ccgataatca
    30001 ccactgataa ctttgcacaa caataacacc catcttttca gcttcatcca aaaaatcttt
    30061 tttggcaaaa tgggtaacca gtaatcgtac aacctcaaca tcaccaaatt cagcttttac
    30121 agcattcacc ttatcttcaa gttcagaaaa aacatctttc cgccaggtag attctaaccg
    30181 tatttttgat tcacctacta taaaaacctc tttaccgtct ttcatagctc tggcaaaaaa
    30241 atttatttct tttccaccta tttctgctct tactattcgc tctttgatag tatatccata
    30301 cttttccttc agtaccgcag gcaaatgcct atatgcttca ttttcaaatg catagctaaa
    30361 acttcttgat aaccctccta attctactct tgtatcatgt aatcctgcta caagactttt
    30421 aacttctatt tcagtacgtt tttgagcatc agcaagctct ttttgagctt cagctaactc
    30481 tgctactcgt tgttcagtct tcttctgtgc ttgtgttaac tctttttggg cttttgcaat
    30541 atccttaaca atagatttta actctgaaaa atcttctttt gttacatgaa cctcattgat
    30601 gcgatcatca ataattttaa caatctgcct cttgacttct ggtgttatcg taaatcgagc
    30661 tgacggcata gtatccccct ttttattttt atctttcaat attttacccc tggtactcat
    30721 caatgatact atatataaat actgttttaa tttcaagaaa aattatagac ttctaaaaaa
    30781 catcttccgc accccataag taataaacaa atatagttat accaatagtt ttgattcaaa
    30841 aatgtttcaa aaattaattt tcactataca ccctttgatt ttgtaaattt aaaaaaaatt
    30901 gtaaataact atcgccaata aaaatatttt tgttgtcagt atttaagaat tttattatat
    30961 tcttaatata tatttcaata acggaggctc tttaatggct ttaattcagg aagttaccga
    31021 tgcaacattt gactcatcag tgttgcaaac aagtggaaaa gtactggttg atttttgggc
    31081 tccatggtgt gtcccctgta gattacaaac gccaatttta gaaaggcttg tatcaaatgg
    31141 catcaatgca aaaatagtta aatgcaatac cgatgttaat cacggtactg cccaaaagtt
    31201 tggcataagc gctataccaa ctcttatact ttttgacaat ggtaaagaaa ttgaccgtat
    31261 ggttggtgtg cagcccgaag aagtcttgaa gcgaagactc caatagcccc ctactgttgt
    31321 atcaatacgg ctgattactc agccgtattg tttaatgaaa aaaatttaca aaccgttaag
    31381 caacccaatg caggtatatt tttaccaagt atttttattt aacataagaa aagatgcgcg
    31441 aattatgttg aaagatacct acagaacatc ccatgacctt acttatgaca tcttcattaa
    31501 gcaaaatatt tttagtgcca gcatacatac attgcccatt attcaaaagt agcaccctgt
    31561 ttatccatgg cattatttca tcaacatgat gcgtaacata gataatatgt ttacctgttt
    31621 taatcagcat ggttaattgc tgcaaaaatt cttcgcgagc cttcatatcc agaccattgc
    31681 atggttcatc caggataaga atatccgggt cataaacaag tgcccgcccg atgagaacac
    31741 gtttttgctc cccatacgaa agcttgccaa acggagtctg tgcatagtgt accagttcaa
    31801 gtgagtgtaa aatagtcaat gctttttgtt ccatggcagg cgatagctcc tggtataagc
    31861 ccatgctgct gaaaaatcct gatgcaacta cttcagcaac catggtttcc tgtgggtagt
    31921 gtgtttgtag ataatcactg acaataccaa tgtgtttgcg aacatcccat acattgacct
    31981 gccccaatgt ttcacctgca atgacaacct gtccctctga gggaaaaacc aatgtggcaa
    32041 ttatgttcat taaaaaagat ttccctgaac cattaggtcc aaggacagcc cattgatcac
    32101 ccgtattaac agtcagattg atatttctta agattatggt tgaattacgc cacaatgata
    32161 cattatgtaa tcgcaaaagc tccatagtat atgaaatagc acatatagct atggagcttc
    32221 aaatttttta aaccatttac aatgttggta tatttttaat tctatcctct ttaatctttt
    32281 tttccttggc tgctttttct tgttcagctt tctctttttc aaccttttcc tgttcagctt
    32341 tttcctttgc taatttttct gccaaaattt tagcttcttg ttcattttgt aatttaagct
    32401 ttaatgcctt tgcttcttct gccttcttta acttttcttc atttttgcgc tgttttgctt
    32461 ctttatccgt ttcaattacc ttttgaatct cctgtttaat ctcctcttga actgttgtaa
    32521 taacaggaat atctttaacc tcctgtttta tttctttaac agtttcttta ggtatttcag
    32581 tgggtttgat ggtttctttt attgaatcta taattttttg ctgttcctgt tctgctacta
    32641 cctcaacttt attctcatca atcttttgag atattgttgc agcagttttg gtatccagag
    32701 taaccgtttg gttttcttct actatcactt ctatatcttc aattaccgtt tcattattaa
    32761 ccggtttaac cataacctta ccagaaacaa catcaactct ggaagctttt tcatcggcaa
    32821 ccaatctgaa tgaagttccc cgcactgcag ctataacggt ctttgctttt atggcaaagc
    32881 catcaccctt ttttaactta cctaatgtag caaatgtttt gcctttctct aatgttaaaa
    32941 caacatcatc gctaccagct ttgataagtg agcttacaac aacagtggta ttttcattaa
    33001 cacgaataat accactattt cggtatagga ggtcaacgac agagtttgct cctgttacta
    33061 ttgtatcact aatagtaaga acttgcccaa cagcaggagg cttattcccc tcatgtgagg
    33121 taatggtaac atcaccaaaa aaggaattga tggtaacaat agtatcaacc gcctcctttt
    33181 ttgagcatgc agttagtatc aaaaacaata ctaaaattat acttttatat ctcatgctat
    33241 tcacccccat tcaataaaat ttatcacatc atgcttgcca taatcaactc accaacacat
    33301 ttcccatcta cagtgcaaag gacgtgacga gattatttcc gcgcagtttg cgtacagcat
    33361 aatgcttcta tggtaaactt ttgcaaataa cgacaccata ccacaactgc tctatagtaa
    33421 atgcaaaaat tgctttttgt acacagttta taccagtgct atgatactta taaatcacta
    33481 taaaagtgca acaaatttta gactacatca gttattatat tttatgaata ttgactgctt
    33541 ttacacctgt caacaacaat taatggctat ataccataaa aaaatagtta tcgttttata
    33601 tcttgacatt ctgaactgaa tataaaaaaa taatttattt gctatagcaa tattgattgt
    33661 tatacatatg gaatggacat gtatggaccg gattacacaa aatgataccc tcatagacac
    33721 gctgggcaat tgtaccattg attcccctct tacaatacca aaatttataa gcgactcaca
    33781 gcgtattgca gtggacatag aggctgacat tattgaacaa tctcttgaaa aggggaaaat
    33841 tccctcattt gaaaatgccg gacctcgtaa aaaaatattt tttaaacctc aaaacaccag
    33901 agcagctatt gttacatgtg gagggctttg cccgggtatc aacaatgtta ttcagggcat
    33961 tgtgcgtatg ctaacattcc aatatggcgt taaaacaata tttggagtaa aatatggttt
    34021 tgaaggtctt gtcccacgtt ttgggcattc atttattgaa ttaacacctc aatttgttca
    34081 tgacctccac gaaaagggtg gaacagtatt aggttcttca cgaggaaggc aggatgaaaa
    34141 ggagattgtt gatacactgg tagctcatga tattaacatt ctttttgtca tcggtgggga
    34201 tggttcatta cgcggtgctt atgatatata tcgtgaagta aaaaaaagaa acctgcctat
    34261 agcaattgtt ggcataccaa aaactattga caacgatata tggtttgttg acaaaacctt
    34321 tggattttca accgccgttt ccactgcagc acaggcaatt tatgcagcgc atgctgaggc
    34381 tataggtgct aaaaatggaa ttggtattgt acgtgttatg ggtcgtgatg ccgggtatat
    34441 tgcagcttat gttacattgg catcaaatga ggtaaattat tgttttattc cagaggttca
    34501 ctttgattta gaaggagcgc acggtttttt acctcatctt gagaaacgac tgtatgctgc
    34561 ttctcatgcg gtcattgtag ttgctgaagg tgcaggccag gaatattttg atgaaccaaa
    34621 actgacagat gtttccggga atataaaata tggtgatatt ggtttacttc ttaataatac
    34681 aattggtgca tactgcaagc aaagaaatat accagtaact atcaaatata ttgatacaag
    34741 ctatctggtg aggagtaccc ctgcctcaag cgatgatgca gtatattgta taatgctttc
    34801 acaaaatgca gtacatggag ctatggctgg caaaaccggt tttgtagtag gtagctggca
    34861 ctcatactat acatacatcc cattatcgct ggtaacaaaa aagcgtaagc gaattgatcc
    34921 ccatggatac ctctggtcaa ttgtaaaaca gagcacctgc caaccagatt ttttttagcc
    34981 agtacaaata atattttttt ataaccacag caacaaacta aaaagagcac cagtgaatgg
    35041 cataaaaaag gacatcgaaa ttcacaaaac agccatcagt agcaattttc cgtaacacag
    35101 cattatatta tatgcagggt tcaataaagc acattacagt atgatacata aaaaaatgct
    35161 atgctatagt atgtgatata aaaattttta attgtatttt tatttatttt attttatttt
    35221 tttagaattc atattaaatt attgactata ctggtatttg agatatgact gctaatgata
    35281 tggatcaact ttttaaaata aaacagctct atgcaaacat cggcaagctt ataacttctt
    35341 ctctggagat agaacatata ctggaaggca tcatggaaga agtccacacc ttttttggac
    35401 ccgataactg gagcttgttg cgctatgatc ctaattctga ggaactgtac tttgtcatat
    35461 cgcatggtat tgattttgac agtataaaaa atattcgtct tcaaaagggt gaaggcgttg
    35521 cagggtatgt ggtagaaaca aaaaaaccgg tttttattcc caacaccagt catgataccc
    35581 gatttagtga taggattgac cgcattaccg gattttctac taaatctgtt atggctgtac
    35641 ctctcatatt tcgtgacacc atgtatggtg ttattgaact tattaataaa tcaacaggtg
    35701 ggattttttc ttctgatgac tatctcatat tgcaaactat tgcagacttt ggcgctatag
    35761 catttgctaa ctcactgtta tacgaaaaag cattaacgtt aacagcactt gattcactaa
    35821 caggacttta caacaataaa aaactcaacg atataattgc atcatggtca aaagacgaag
    35881 cccatatccg acgagatgac actacccacg tcatagcagt attgaatata gatataaaag
    35941 gccttaatga aattaatact atagctggac acagggatgg tgatcgtgtt ataaaacata
    36001 tatcacgaat acttaaaacc agcctgcgtg aaaacgacat gactttcaga acaggcgggg
    36061 gtaattttat ttctatcatt aattgtgata attattttga tatgaaatcg cttgtggaac
    36121 gtatactgca caaaatcagc gccataactg ttcccgatta tcctgatctg catatacagg
    36181 taagtcatgg actgagcatt ggcacgcgta accgcatcaa tgagcttatt caagaaaccc
    36241 ttgaacaaaa atcatagata tcactacatc tggattcatc aatgtgtttg caatgattaa
    36301 taaacattaa ccctttttac gaagttgttc aaaaaatgac ttcagcaaat ttttgcactc
    36361 ttcttccagt atcccaaaaa taatctgtgg tttgtgatta agcacaccat tgccacatac
    36421 attaagaact gttccgcatg caccaaaacg tttatcctta acaccaatat aaagtttctc
    36481 aatacgtgca tgaacaatgg caccggcgca catagcacat ggctctttag ttacatacat
    36541 gcgacagccg tttaaccgtt cgtttttcaa taccatggca gcctgcttaa tggcaattaa
    36601 ttcagcatgt gcagtggggt cagagcattg ccgcgttgtg ttatgtgcag tggcaatgac
    36661 accatcttta aaaactatcg ccgccccaac aggtatttca ccctcatcat gtgctttttt
    36721 agcttcctca agggcgatag tcatataaaa ttcaggatta ttgtcattat tgttcatggt
    36781 agtttcccaa tttcgctatt ttgccagctt cttcaaatgg atacataata gcctcttcac
    36841 tatactatga gtaaccatac agataaaact gtctaccaat caacaaaaaa taatgagagt
    36901 aactatcgcc aaacttttct tttatccata taatacaatt gacaccataa gattatatat
    36961 tatgtatgtc attaatacag taataaaata ttatttttgc tatactctat ttataatatg
    37021 ggaggtttgt tatgatgcgc aatccactgg tagatcatcg agatgtatat tttgttttat
    37081 ttgaagtgct tgaaacagac aagctgcaac aatttccaaa gtttgctgag catgataaag
    37141 acatgtatga agctacaatt gatttagctt taaagatggc aacagaagag gtttatccag
    37201 taaataaaga agcagacaaa ataggtgtac gctatgatcc tgatactaaa aaggtaataa
    37261 ttccacaagg ttacaaaggt gggttacgaa aatataatga agctggtttt gttggcatga
    37321 tcgaagaccc tgaaattggg gggatgggta tgccaggagc tattgctatc gctgtaaacg
    37381 agatattcac gtcagcaagt ctatcgttta ccacctatcc cggattatca catggtgcag
    37441 cagcactcat tgaaaatttt ggcactgacg aacagaaaaa ggtgtactta gaaaaaatgc
    37501 tgagcggaca gtggggcggc accatgtgcc tgaccgaacc tgaggctggc tcggatgttg
    37561 gaaatctaaa aacgaaagcc ataccacaac ctgacggcac ttaccgtata cagggacaga
    37621 agatattcat tacatcaggc gacaatgact tttatgaaaa cattatacat ccggtattgg
    37681 cacgtattga aggtgatcct cctggtacca agggcatctc tatatttatt gttcccaaat
    37741 acaggattaa tcctgacggt agcataggtg aattaaatga tgtagtatgt tccggtatag
    37801 aacacaagat gggtatacac ggttcggcaa cctgttcgct caattttggt gataatggta
    37861 catgcgctgg atatctgtta ggacagccac gcaaagggat gaagatcatg tttcacatga
    37921 tgaatgaagc ccgacttggt gtagcctgtc aggggctggc attatcgtct actgcatata
    37981 tgcatgcagt aacatatgca cgcaaccgca ttcagggagt tcatgttacc cagatgctta
    38041 atcccgatgc acaaaaggta gccatcattg aacatcctga tgtgaaacgc atgttgttat
    38101 ggatgaaagg ttatgtggaa gggatgcgca tgctcaatta ctatctggct cataatgcaa
    38161 ccttaattga agtattagac ggtgaggcaa aaaaggaggc acaggctata gttgagatac
    38221 tcattcccat tgcaaaagca ggctgcactg attcggcagt gcttataact tctgaagcca
    38281 tacaggtata tggcggctat ggatacatat ctgattatcc agttgaacaa tatatgcgtg
    38341 atgcaaaaat tactcagata tacgaaggta cgaatggcat ccagtcaatg gatttaacaa
    38401 tgcgaaagat actgatgaat cctgaacagt acaattatac tatcatgaag aaaagaatgt
    38461 cagaagcaat tgccaatgca aagggaattg ttgaagaaaa atacattaac cttgttgaaa
    38521 acggcatgca aaagatggat gaggtaattt tgatgcttaa agagcagatg gctgcaggga
    38581 agtttttaca tatctttgca gcatgcacgc cagtacagca ggctatgttt atgctaacat
    38641 tagcgtggct acatctgtgg gctatgactc ttactattcc gaagatgaaa gcacttgttg
    38701 gcgaaaagaa aggggaagaa agagatgcgt ttttatcaga aaacgaagaa gcatcatact
    38761 attcaggcaa agttttatct tcacagtttt atttaggagc agaatttcct aaattttttg
    38821 gaaaaattga tgcgatacta tttaatgaaa ctgcgatcat taaagccagt aaagatatct
    38881 ttactggagc cttattagag taaacaggat caaccatcaa aataatatga gagggtgttg
    38941 gcatcctctc tttttttgtt taaactataa aaatcatcca ctcattttat caaaaaccgt
    39001 gtttttcata atttttattg taaaaattgt ttacatcctt tttgaagttt tataaatatg
    39061 tagcaataga attttatttt tgacatataa acagattttt atacttttta atactctgga
    39121 gggaaacaat ggattttgaa accacaacct gtatttcagt agagcatagg gcactgcttt
    39181 cacaatactg tgaaaagctg gatatacccc tgcggacgtt tattgtttac tgtatcatgt
    39241 atgctgcaaa acatgaaaaa aagaaggaag ttgcctttaa acgtatatcg tacagaaaaa
    39301 gaaacactga taatccatgg aagagggtgc atctggagct gtatcagtcg gaatatgagt
    39361 tttttcttga tgtgaagaag ctgtggaaga tgtcgcttgc caatattatt gcgtattgtg
    39421 ttgataacgt gctttttgaa ttttttgaat atttcagcaa gcagatagaa aagataaaga
    39481 ccgataacta tcttagttac tatgaaaaca ggtcgtattc attcaacttt taccgggtaa
    39541 aaggcatcca ttgcctcaaa ttctactggg gaccgccacc ggagatgctt ggacaaccac
    39601 tcacccaata aaaattaaca ttaatcattt agtctgttat tgagcatgca gtatcatggc
    39661 atatctgtat tttaaagata tgcttgatgc agaaaaaatt attttcacat tgtataatat
    39721 tatgtaaaaa ctatctgcca attataaaaa tgactctata aaattcttta cctcttccct
    39781 gccacagtaa acaccaaaat gcccctcgca taaaatatca gcatcaagcg acagtaaaaa
    39841 ttccagcgac tgtatataat catctctgtt tgatttcaat acatcattca gtggtccatg
    39901 aacatcctgt ccaaaaagta caagtttgcc ttctgattca acagttaacg ctaccgaacc
    39961 tggcgaatga cccgggatgt gatacatgtt cagcgttaag ctcccaatag caaatgactt
    40021 tttactgtca ttgaccttga tgtcaattgg acagggagta agaaaactac cataccagtt
    40081 tgccgctgtt acatattgat cacctgtctc aatatactgt gcatctaatt ggtgagccac
    40141 caccttgcag ttgaatgtgc ccctcagctt atgcgcacca ccaacatgat caaaatggca
    40201 atgtgtcaaa aaaagatagt gtaccgttga tgcatcaata ttttcttttg cgatattttt
    40261 taaaaccctg tcatgtccat cgccggtccc tgcatcaatg agggctgtaa ccttcccatc
    40321 agtaacacaa tatattgctg catcagaagg atgacttaat gttgaaccac caatctgata
    40381 tacatgctct gttatctgca tagtaacgat cctgtatata taatttaaaa acttattcga
    40441 tacacgcact cattctaaat atagaaagaa ctgtgcactg ctgtagctaa ccggttatag
    40501 tgtagtacac ctgccatcca tcactattaa taggctacgg tgctccgtct ttgcaactca
    40561 ttgaacagta cctcgcgcac cctatctgca ttggcttcta attctatagg cctgttagca
    40621 taaatgctct tcacatcggg aagggttccc tgatggtatc caaccttgaa ttcgctaaac
    40681 ttcaatccat gtgcagttga tatgaccaca gttttgtcgg tctttgatat aacctgtttt
    40741 tcaacaagct taagcaacac agccaatgca acgcctgtat gaggacagct atacatgcct
    40801 gttctgtcag ctaaagcagc agcatcggaa agctcatcct ctgatgcctg ctccacaatg
    40861 ccgtcaaact gctgcaatgc cttgatagcc tttgttaccg aaaccgggtc gccaatctga
    40921 attgcggatg ccagtgtctt ttgcggagta atgggtttaa attctttaaa accattacgg
    40981 tatgctctat acagtggatt ggcattttgc gcctgtgcaa gcacaatacg cggttttttt
    41041 gttataagtc ctaactgcaa catcatgtca aaaccattcc ccagagcaga aacattaccg
    41101 aggttgcctc ccggaataat gataacatcc ggcacctccc agtcaaactg ctgaacaatt
    41161 tcaaccgaga ttgacttctg cccttctata cgcaacgaat tcatagaatt agccagatag
    41221 atggtattgt cttttgttat ctcctgcaca atcctcatgc aaccatcaaa gtcagtatca
    41281 agcgacatga cgatagagcc attactcatg ggctgcacca gttgagcggt agaaattttg
    41341 ttccgtggta aaaatacaat cgatggtatc ccggcgtatg cacagtatgc tgccaatgct
    41401 gccgatgtat caccggttga agcgcatgca accgctttaa ttggcttccc ccgcatgatc
    41461 atttcattaa ccatggatac cagcaccgtc atccctaaat ccttaaatga accggtatgg
    41521 ctgttgccac acatcttaat ccacagatca tgcatgccaa tcatagcacc aaagcgttta
    41581 gcccagaata aatttgtgtt accttcaaac atggaaacaa tgttttcatt gtcaacctca
    41641 gggcacaccc attccttttt gctccagacg ccgctcccgt atggccattt tgtggtgcca
    41701 acacggctat taaaaagctc tttccatttt tttggcgata gttcccgtaa acggtctcta
    41761 tcgtgataaa cctctaacag cccaccacac tttttgcacc tgtatacaac ctcatctaat
    41821 ggatactctt cactgcaacc agctatacag cgaaaaactg ctctatagtt actgtcgccc
    41881 cccataacta ttcacctgta ttatcaaaat cttctattgg aataatcatc acatgaccat
    41941 taacaaaatt gaaccgttca atttcgtttt ttgcctttcg cattcctgct tcatttgctc
    42001 tatgcgttgt gatgacaata ggaacatatt cagtcattgc ctccttttgt attaccgaag
    42061 caattgaaat attataccct cccagtacac cagctatctt tgaaagtatg cctgccctat
    42121 cctcagaata caaccgtata taatacctca gcatcctgta ttcaggcgat atatattctg
    42181 catccttttc aataaatata caatccttcc caccattttg cttctgagca atagaaacaa
    42241 tatcactgac taccgcagat gctgtaggga agcttccggc acctttgcca tacagcgtca
    42301 ccgggccggt catatcaccg tgatacataa tagcattaaa ttcattgcgc accgaagcca
    42361 gcggattagt aagcggaatc atcaccggct gcagccgtat gtcaagctta caatcaagca
    42421 tacggctaac acccagaagc ttgatggtat atcccatctc atgagcatac gtaatgtcca
    42481 gagcggtgat attggtaatt ccttcaacat aaaccgactt catggaaaca ccaacattat
    42541 aggctaacat cgatagcagc acaatcttat gcgctgcatc gtacccttca atatcaaagg
    42601 ttggatcagc ttcagcatac cctttatcct gtgccaatgc aagtgcctcg gcaaacgaca
    42661 tgtcatcttc tgacatgcgt gtcaatatat aattggtggt accatttagt attcccacta
    42721 ccgactgtat ggaattccca accagcccat tgcgcagtgc cgcaacgcag ggtataccac
    42781 caccaatggc agcctcaaat gccagtgacc cttttactga catgcttgct gcaattattt
    42841 catcaccatt ttcggctaaa agctttttgt ttgctgttac aacattttta cccgccttca
    42901 gtgctgaaac aataatagat ttagcaggtt ctgttccacc aataagctct atcaccgtat
    42961 caatgctggt gtcatatatg atgtcttccc atttgtcggt aaccgggatg gcaaccatag
    43021 aagcaatgtt gcgcacatca catatcttta ccagctcaat atctattcct gtttttttta
    43081 aaatatcgcc ccgatgtttt tctaaaagat gataaacacc acttcccaca gtaccaaaac
    43141 caataagccc gcatcgcacc ttcatagcct ttcctcattt tttatagatg atagtcattc
    43201 ataatccggc aacagtttaa ttgtcaactt tatttatagg tattgactta tgcagcatat
    43261 atgttgacag gtttttgaca gccgattttt gtctgcccat aatagttaac aatatctggc
    43321 gatagtcatt ctaaacaaag tgtctgtcat tcaaagcaaa gcgaagaatc atggtgctgc
    43381 actcaaacgt ataaaacggc atccatgcag tttatattaa ttttttactt tataagaata
    43441 tgccattata ctataatagt accatgggtg aaaaaattac aattatcaac ctccacaaat
    43501 catttggaca acaggttgtt ttttccgggc ttcatctttc ggtccatgag ggtgagatac
    43561 tgtgtattat aggcaaaagc ggttcgggaa aatcagtact gctaaagcat tgtattggca
    43621 ttttaaaacc tgatagcggc agcatacttg ttgatggtca aaaaatttcc aatgatgata
    43681 gcgatgcatc tattataaag aaatttgggg ttcttttcca gggcggtgcg ctgtttgact
    43741 caatgaatgt atatgacaat attgcctttc ccttacgcag gcagggagca agcgaggaga
    43801 taatcgcaca aaaaatccct gaattgcttg cccatgttgg gctaaaggga attgaggcaa
    43861 ggatgccttc agagctttcc ggcggcatcc agaaaagggt ggcgcttgca agagctatcg
    43921 ccaaaaatcc tgaaattatg ctgtacgacg aaccaaccac cggcgtggat cccataaact
    43981 caggttcgct gtacaggctg atagcataca tgcgaaagat attccatgtt acttcggtga
    44041 tagttaccca tgatatgagg cttgccttta ccattgccga ccgcattgca atgcttcatg
    44101 atggcacaat cattgttgag ggaacgccac agcaaataaa ggaatgtcag gatgcagttg
    44161 tacaacagtt tgtttcagga aaagcaaaag tataaattat ccattaattt tatttttaat
    44221 cctgctttta tattcaccaa tctcgttttt caggatttca aggtccttca tcctgtcgtc
    44281 aattctcctg atgtgcaaat ctaaaatctc caataagcgt ttaagcacaa tatcatttgt
    44341 tttatgtatc tgccagatat actctaactc gtgcatctca gaaagggtca atccaagaat
    44401 cttcaggcgc ttgattaatt tcaagcgcct gacatcatca tcagtataga tacgtctgcc
    44461 attgtctatg cgcttcaccg agttgagcag tcccagctct tcataatacc gtatggtgcg
    44521 cgtgctcatc tctaaaagct ttgctaactc accaattgaa taggttgtca cttttacttc
    44581 aatcacctgt tattattttt aaaaaaccga agctgccatg ccatattttg atgcaaacat
    44641 ctcacgtaat ttatacttct gaaccttacc acttgccgtc atggggaatg cttttacaaa
    44701 ttcccagtat tttggtatct tatggcgagc tatcctgcca gtacaaaaag caaccatttc
    44761 ttctgcactg gacgattgtc cttcctttaa ttggatgaca gctaaaacct gctcgccata
    44821 cttttcatca ggaataccta caacctgtac atcctgaata ttttgattcg tcaacaaaaa
    44881 ttcttcaatt tcacgcggat agatattttc accaccgcgg ataatcatat cctttattcg
    44941 tccggtaacc ttaaaatagc cgttttcatc aaccgaacct aaatcgccag tatgcaacca
    45001 gccgtctttg tcaattgcat ttgcagtagc ttctggcatc ttatagtatc ccttcattac
    45061 acaggcactg cgtgtaacaa tctcgccctg aatgttgggt ggcaaatctt cacctgtttc
    45121 aggatcaacg attttacctt ctatatccgg tagcagtctg ccaatagttg acacacgcag
    45181 actcaccggg tcatcacggc gtgtcatcgt cataacaggc gaggattcgg tttgcccaaa
    45241 tgcaatgaca atatccggac aatgcatgcg tgttctaacc tgattcatag tttcaacagg
    45301 acacggagcg cctgccatta taccagttcg cagtgatgac atgtcatagc tgtcaaaatc
    45361 gggatggtta agcattgcaa tgaacattgt gggaacacca tttatggctg tacacttttc
    45421 actggcaaca gcctgcaacg atttcagcgg atcaaaactt tccataacaa ccatcgcagc
    45481 accatgggtc acacagttta atgtactcat aacgcatcca aaacagtgga acagcgggac
    45541 atgaatgagc agcttatcat ggggtgatgt ccccataaca tcaccaacca ttagcccatt
    45601 attgactatg ttataatgag ttaacataac accctttgga aaacctgttg taccggatgt
    45661 gtactgcata ttgataacat catcaacatc gcactcgctg attcgtttgt ccagtgccgc
    45721 atcacttata gcattcccca tcattaccat gtcatgaaag gtaaacattc cgggagtagc
    45781 atcgcgcttt ccaatataaa caatattttt aagcatcggc agttttggct catttatcat
    45841 accgggttga gcacaatcta aattagggat taattcccgt acaatctgat ggtaattgct
    45901 atctctgtat ccttcaatca taaacagcgt tgttgaatct gattgtttta actggtattc
    45961 cagttcatgc gacttataac tggtattcac cgtcaccagc acaccgccaa tcatacccag
    46021 tgaaaactgc aaatataccc attcaggtac attggttgcc cataccgaaa catgatcacc
    46081 tttttttaca cccaatgcaa ttaatcctct ggcaacctcc ctgcatttgt gataaaactg
    46141 cttatacgtc agccttaccc caaattcagg acataccaat gcctcatgat ctggatattt
    46201 ttccacagta tcataaagcg ccttccctat cgttaatctc ttaaaagcca taccaacctc
    46261 tcctttttta aaatatatat agcttataat aatacaacaa aataattttt ataagaccag
    46321 tttacgtaaa ctgttatatg actgcatggt atgtcaacat aattacataa ttttttatca
    46381 atttttgaaa tttacaaaac agcagacact acctgcccgg tatacaaccc taccactctt
    46441 ttttttattc tttgtgcgat agttactgct tattgacatt ttgtgcaata aggtatatcc
    46501 tgtcaatatt taattggctg tccaatcaat tttattaatg tattctgcac catcaaatat
    46561 atttttgcca gataaaaatt atagtgcaat caatgtatta tataaacaat catgttttac
    46621 atgaatcata acaaaccttg catacacatt ttatggaagg cgttgtaaca ggcaaagtat
    46681 aactgtcatt gagtaacctg attacattac aacaaggagg ttttatggat tatactattt
    46741 tgacagcacc atgcggatta gactgcttta actgccccat gtatctggca gcaaatgatg
    46801 gcagccttcg catcaagatt gcaaagtcta tgaatatcag tgatgaacag gcagtgtgca
    46861 agggttgccg taatgaaaac ggaacgattg gttttttaaa catgaaaaag caatgcacgg
    46921 tatacgcatg tacaacttca aaagggatac atttttgcca tgaatgtgat gatttcccct
    46981 gtgataacct gcacccctat gccgacagag catcacaggt accccataat acaaaggttt
    47041 tcaacctgtg ccttataaaa aagatgggtc ttgaaaagtg ggcacaagaa aaggcaaagg
    47101 atgtgaagaa aatctatttt ggagggaaat ttacactgac ggaataaatt gagtggtact
    47161 attactgtag tatatttatg tgaattaatg tggcatccgg atttatatat ggcaactcca
    47221 tcgtattttt aataaatttt ctcctagttt aggtttccta aacacaaaca aatgttcatg
    47281 cataattaaa taaaacccga atcgttttgc attttcttcc catctttttg aataaacaca
    47341 attatgctgt gtttttatta tttcttcttt taagataaat cccaatttta aaaatcgttg
    47401 taataaaaaa tatgaaagtg gtatatagtg ctgacctttc cttgtatcgc ctattaacac
    47461 tgcacaatat ttatcatgtt tcaagaccct taataactct ttggcaataa tttcaatttc
    47521 atcaaaaaat ttaccaacat tagaaatatt tgataaatcc tctttaatga caccctgaga
    47581 atattttatt atatttaaat atggtggatg aaaaataatg aaatctattg actcatcaga
    47641 aatattagac agttttcttg catcacctac ataggcttct tgtttacaat taccatcttc
    47701 tacctcgaag cttaaggctt tttttgatag ttcaatagca taaggattga tatcaattcc
    47761 tataatattc ctacataaaa gttttgcctc aattaatgac gttccagacc ctaccattgg
    47821 atcaagtatc caatcatatt gacctgtata tctttgtatc aaatttcttg ctacttgagg
    47881 tgcataatta cctctatacc ttgaattatg agtagcccat ttcccccttt caggaaatga
    47941 ccatacagta gttatctctt ctttaaaatt attaagttgc atattatgac attaaacctc
    48001 ctcctccttc ttcagctttt gttctggcac tttcccaacc agtttgtcct aaaatactaa
    48061 ttgatttggg tggatttaat tttttaaaat tttcacttct tggtttgcat acaacaacaa
    48121 atggacattc atttacatcc gggaatatag catctttacc tgtaacaaaa acattttttt
    48181 gaatggcaat atatccttca ttatctgtac atggtaaaac ttttttaata attgttgccc
    48241 tacttttttt acaaatctga catgttctgt ttcttgatga atgccatcga aaaatatgtt
    48301 tttctgcttc acactcaaaa
//
LOCUS       DAOOJH010000011        47372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_862_length_47372_cov_10.640552, whole genome shotgun sequence.
ACCESSION   DAOOJH010000011 DAOOJH010000000
VERSION     DAOOJH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 47372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47372
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_862_length_47372_cov_10.640552"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLJ75_02920"
     CDS             complement(<1..458)
                     /locus_tag="PLJ75_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD04198.1"
                     /translation="MEALPWYREYKILGIPKTFKPYPDKPVYDILEIAAKQYRKNGLI
                     QENFFLSYPELLIYVNKLTSAFHAFGLKKNDIIATILPTSTEFIICDYAISRAGLIHL
                     PCSALEPAEALEHKFKEAKPKAIICTVIGDHITVVKNLMKKFSLQFIIVTT"
     gene            complement(538..1095)
                     /locus_tag="PLJ75_02925"
     CDS             complement(538..1095)
                     /locus_tag="PLJ75_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPD04199.1"
                     /translation="MAITSNTTVEQFLTEIMPKEALGMLQNSNAPKELNGTEITMVVE
                     VDGKSYGFKVNDGKDITPCGDIPNAMLRLKISEADLKKMIDTNNLDMLLGMQKDLTRA
                     RYNALSSLKGSFIAKLKNDDGSEFTIEAILNGATSPQSIFRMSTKDSAALMKKETNPV
                     NLFMSGAMKIEGDMSFAMATQPLFT"
     gene            1312..1926
                     /locus_tag="PLJ75_02930"
     CDS             1312..1926
                     /locus_tag="PLJ75_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04200.1"
                     /translation="MSRADETRDKILLYAKKLFSEKGYYSSQISDIVESAKIGRGTVY
                     QYFSNKEHLFISLLERFLIEWEEYIKELSVSEDLSIISHYTYLKRRLYKAFKFFFSDR
                     DRANIILRAGIGLPEHFEQKIQHFENKLINMIIADLAIAVEFNHIDMNEDLSFAANLV
                     AGGVFRLAHYYLLIESNDEVLLQNLDDISSKAAHFIGNGIFSKK"
     gene            1990..3087
                     /locus_tag="PLJ75_02935"
     CDS             1990..3087
                     /locus_tag="PLJ75_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD04201.1"
                     /translation="MVEEISFALSEEQQMMQDTVRQLVQATIADGIHQMDENRQLDKQ
                     AVQKFWELGIIQSMIPEEFGGYGMDSSPVLHTLVLEELAYGDMTFAIYAMLPAVFVLP
                     IVHLGAKEQKELYLPEFCNATFVPATLAINELSVAFDVWNCATTAETKGDKYIINGSK
                     CFVPLADESNFMIVVAQCEGNPELFIVTRDNSGIKVGEREKNCGWWALPTFEVTFENC
                     IIPASHRLGDSTAITWLLQRTRTALSAIGTGISRASYEYAKKYAKERQQFGEPIASRQ
                     AIAFMIAEMAYEVDAMRLLSWKAASTIENNLDATRLSYLAKMYAGEMTMKITDYGVQI
                     LGGHGYIRDHPVERYYRNGRSIALCEGMAII"
     gene            3106..4302
                     /locus_tag="PLJ75_02940"
     CDS             3106..4302
                     /locus_tag="PLJ75_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD04202.1"
                     /translation="MIQLVTPEYLVTLQNNMRKLAEGVLRPISRKYDEAEHEYPKELD
                     MFRNIKVMARPKKKKDGEKSSSGEEKKPKRGANLGMVATIEEMCWGDAALLLSFPNAG
                     LGNAAIIAVANDEQFERFGNKWAAMAITEPGAGSDSGAITTTATRDGDFWVLNGEKIF
                     VTCADRSEVVVVWATVDKKAGKSGIKSFVVEKGTPGFTLEHLEHKLGIRASDTGTFVL
                     SDCRIPYNNILGDPEVKMSTEGFKGVMKTFDNTRPMVAAMATGIARAAVEFTKEQFEQ
                     KGYTFDYKKNTANISAVERELYMMEANVEAMRLLTWRAAWMADNGQFNNLEASMAKAK
                     AGRYATLVTQRCCELLGPVGYSTHYLAEKWMRDSKIMDIFEGTGQIQHLIVARNVLEM
                     SSKELK"
     gene            4345..5508
                     /locus_tag="PLJ75_02945"
     CDS             4345..5508
                     /locus_tag="PLJ75_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPD04203.1"
                     /translation="MNTIDDVKNIAVIGSGAMGHGIAQVCAQNCFPVTMIDIKQEFLD
                     TALKKVKESLDFLESKGKLNGTVDEVMKKINVTTDMKKGVSDAQVIIEAVPENMKLKQ
                     EVFSQASAACAADAILATNTSTMSITDIATAVNNPERFAGMHFFNPVTRMKLVELIYG
                     AKTSEKTIDILYELAKKIDKIPVRVLKDRPGFIVNRINAPNQALLSAILDEGKIHPAQ
                     IDGKMKKMGVKMAPFETADFVGLDVFCHTLEYYAQTLSPQYKPGKFLMECLSKGNLGM
                     KSGKGIYEWKNGRAVIPEMPDTEEITPLHLMAVQVNEAVKVFKEGIAANVADIDDGVK
                     YGMNAFAGPFALASGVQPQQLTDCLNYLSQRYKLDILKPEPEIIDGSFKTIGR"
     gene            5519..6286
                     /locus_tag="PLJ75_02950"
     CDS             5519..6286
                     /locus_tag="PLJ75_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPD04204.1"
                     /translation="MSDAVLLEQKGMVAILTINKPKANQLSHDVFETMRKYLDTLEID
                     NNIRVLVITGSGDKIFCAGADLSQGFGDFSAVDFLKRGQDIWNKIEDYPKPVIAAING
                     HALGGGCELSMACHIRIMKKGARIGLTETNLGIMPGYGGTLRLPRLVGRAKALEMILF
                     GKQLEADEALALGLVHRVYDEGYMDEVMKFAEELTNRPPLSIKAILKVMSAGPSMSPE
                     MHLKMEREELAKLFTTKDCMEGMMAFAQKRKPEFKGE"
     assembly_gap    6365..6464
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6465..7087
                     /locus_tag="PLJ75_02955"
     CDS             <6465..7087
                     /locus_tag="PLJ75_02955"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04205.1"
                     /translation="FLLNFEESTMAGTGVRENGKQRRLIMEKSQVLFWEKGYAETSMK
                     DIANECGFRPANIYNFFASKEEILFFILKEEMEEIVNPIRFLEYEENDPLQQLRFLIE
                     IHVKITLGEKRASKLLFDTELRNLSAERRKVILDLRDDYNRIGYSILKRGINKGLFRA
                     IDEKIALNLIASMIVRTRLWFSPEDWMTIEDLVEFIFQFALKGLSK"
     gene            7101..7421
                     /locus_tag="PLJ75_02960"
     CDS             7101..7421
                     /locus_tag="PLJ75_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPD04206.1"
                     /translation="MGKKECIRCGACCFVDMVAYATPEDFLRWEKEKRYDILHIIENE
                     NSIWAGDHLISTRDGHLIGRCPFLIMENNGLYSCMIYDTRPAVCKDFEPGVSIMCPLF
                     ALKE"
     gene            7457..8395
                     /locus_tag="PLJ75_02965"
     CDS             7457..8395
                     /locus_tag="PLJ75_02965"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPD04207.1"
                     /translation="MNKNWAVSIIIVNGLIFLVQLLLRNVFVPYDIAGNVVSIDIVTY
                     YLGLIPQLVVEKLYIWQIISYMFLHSTSSFAHILFNMYAVFIFGMPVERLWGSKKFII
                     YFLICGIGAGVAIFTIALITGGMGYYIPTIGASGAVFGLLLAFGVLFPNVEILLFFII
                     PIRARTLVILYGGLELFLELSGGADNISHIGHLGGLATGILYFIVTRKHAIRYKTKGF
                     VAAQMKKETIPDKPDVNYKREAELQRAIVQKIREHGVESLNDDEVQYIRYKEIMIDIE
                     EEEVPCDLQIFLDDNCNDCENRDACFIKIVKSFKKE"
     gene            8584..11298
                     /gene="ppdK"
                     /locus_tag="PLJ75_02970"
     CDS             8584..11298
                     /gene="ppdK"
                     /locus_tag="PLJ75_02970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004364315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPD04208.1"
                     /translation="MTKRVYLFGGNVTEGTADMKNLLGGKGANLAEMARIGIPVPAGF
                     TITTEVCNEYYKNNQKLTESLKEEVTEALRQVEGIMGAKFGDHSNPLLVSVRSGARAS
                     MPGMMDTILNLGINEDVIKGLVKKTGNERFAWDSYRRFIQMYGDVVLGLKPESKDEHD
                     PFEVIMDELKEKRNVKNDLDLTVEDLKELVASFKKLIQEKLKVTFPDDPYEQLWGAIG
                     AVFRSWNNERARLYRKMNNIDDSWGTAVNVQAMVFGNMGNDCATGVAFTRNPATGAKE
                     FFGEYLINAQGEDVVAGIRTPLQITLEGSRRWARENNISEDERKNKYPSLEEYMPEAY
                     NELVNVYKTLEKHYRDMQDIEFTIQNKKLWMLQTRNGKRTARAAINIAVDMVEEGLIT
                     KDEAVLRVDPQSLDQLLHPTFDPKAQRKVIAKGLPASPGAATGKIVFNAEDAHDWKGR
                     GEKVILVRIETSPEDLKGMNAAEGILTARGGMTSHAAVVARGMGKCCVSGCGMLDIDY
                     TKRQIKVDTTILKEGDYISIDGSTGEVMLGKVATMEPEVTGKFGTIMQWADEIRKLKV
                     RTNADTPHDARVARNFGAEGIGLCRTEHMFFEGDRIKAVREMILAPTYEGRKKALDKL
                     LPIQREDFYGIFKAMEGLGVTIRLLDPPLHEFLPQDIETQKEMAKELGVTVDDVKAKI
                     EELHEFNPMLGHRGCRLGITFPEITEMQSRAIFEAACQLVKEGIDVKPEVMVPLVGHV
                     NELIMQKEIIIKTAEVVMKEMGVTVKYMVGTMIEVPRAAITADEIAKVAEFFSFGTND
                     LTQMTFGFSRDDAGKFLKEYVDKKILPDDPFRVIDRDGVGQLIKMACEKGRSVNPNLK
                     LGICGEHGGDPSSVEFCHVVGLDYVSCSPFRVPIARLAAAHAAIRFPK"
     gene            11373..12098
                     /locus_tag="PLJ75_02975"
     CDS             11373..12098
                     /locus_tag="PLJ75_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04209.1"
                     /translation="MRLLIVIILTLSSIAPAYAQYDKAVKQFEQGNYDAALAEIASNL
                     DVSQDFEPNSLNYNLRYLAGHIHAKKGNYESALAHLKRCAEIKKDTIDPIIDIAFIYI
                     DKKSYTDAITWARKAIQIKQSAIAYYILGLSFSGIQSYWQAKEYLEKAIAIDPEMYYA
                     YNELGNVLMHLGKLSQAQTAYSAAYALYPSSAYICNNLALCYAKSGDIKKAKEMIGKA
                     HTFDASNTIITANYNRIINVANK"
     gene            complement(12268..13047)
                     /locus_tag="PLJ75_02980"
     CDS             complement(12268..13047)
                     /locus_tag="PLJ75_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04210.1"
                     /translation="MKYLLITFFYSLFFLFIAVPFSNLFSSEFDELDKPPEGMHKDQL
                     LILGAVSIGMPYGNLITAEEKFTKKSSYQLTPETSKLIYLSHLAFGITISGEYMPADY
                     IGIRSKLRRSIIIQNTNFGSDYKSWKKTLYSDYSIYLGPSFHMTSRKPWDIALSPLIG
                     IAFSQYNPTPIAKELLDGYDGPPSEQFTSLTYGVDLLLSIFFSGGLVIQLGGEWVRNN
                     ITFSSTPNAENPQTHQIFYNKKSAVIDSILFHVSAGYAFYN"
     gene            complement(13044..13661)
                     /locus_tag="PLJ75_02985"
     CDS             complement(13044..13661)
                     /locus_tag="PLJ75_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HPD04211.1"
                     /translation="MKKLQFISLILLISLFSCTSIDVAYTNKQIIQKRMKRVAVLPFE
                     ITGADWGNEFADAISHQFFKSGKVATIEREAIEKILQEQQLQASGLIDEKQAIKLGKL
                     FGADVIIIGRGTALQAYRKNKYEPNCIDTFTLKAINTENGEILLTVRKEPGIAWDWRY
                     RAQYCCGLTLIWDTNDILVESSQYDDIAKQLVKKILLIMENDKNK"
     gene            14027..14629
                     /locus_tag="PLJ75_02990"
     CDS             14027..14629
                     /locus_tag="PLJ75_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04212.1"
                     /translation="MQNATKIIIKYELISNLTHLLIMDNGGNKYYLLEHEHITEMLNN
                     VTHKLYDDLIITNNAYQVVYSMQNKNYYGRRIFDISPQLQQCLMKCNYNDISICEPSE
                     YPVMSGNFNFCVVYPITSNERNKGFLIGFINKIKLYDVLPEDAIIVDKYGMVIIKPEK
                     SILKTIPIPDLQNNYVTFEQKQYAIIEIKYKNIEWYLLSP"
     gene            complement(14633..15877)
                     /locus_tag="PLJ75_02995"
     CDS             complement(14633..15877)
                     /locus_tag="PLJ75_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04213.1"
                     /translation="MKLYITLTFRCDDKNFLLFIQKVIQADCTIQSINYLSSEQNYKI
                     YKAELVYTDRRLFKEKILQWITKECTLIECSDIMNQGIDGGLLKVSSKIPFETPDEFE
                     GKVLGYFELIKDNTITKDTYRYTGISKNIGMIILNEEKNELQPDEIYNYLLIERDSLI
                     ISHCNNYNPQPLLLKYKSKEDIIKTLYSVSGNYALMRVAIMNNIEDISLFHQMSEVTP
                     PIINREFDEIPLYITVKIMNLLKLHNIKLSDSVVGFIGVTTSLLRCTRLLNSIGCSKV
                     LGFDPNEKNLYYFEKESGIATSTDNVFENADILLFVSDICENIDYYKLQKGQLVLSNP
                     AIHFDMLISEERGVKANEYLNIENSYLLLPGIIDMMLMNDIKTLNDNCLINIARALVK
                     ANIDIDNTENIYTIKDIIKNIQ"
     gene            complement(16009..16596)
                     /locus_tag="PLJ75_03000"
     CDS             complement(16009..16596)
                     /locus_tag="PLJ75_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPD04214.1"
                     /translation="MQESKKTNYDVVVLCGSPHKEGNTVAILQLLFKELSNITKAIHF
                     AYELHVHPCIDCGFCHNEFGCIFHDDMDMLYATIQQSTLMIVASPLYFSHLPSPLKAI
                     IDRCQVFWEYYKNNPSQKKQHAMAILIGGGNYYDMFMPGKITLRHYFNSLQYHYDEDN
                     FLLLPNINSKNDIENADIMKKVEQTIHYIHTVLQR"
     gene            complement(16574..17575)
                     /locus_tag="PLJ75_03005"
     CDS             complement(16574..17575)
                     /locus_tag="PLJ75_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HPD04215.1"
                     /translation="MIAIIRFLFIITSAVVTGLYFFQISLQSAIIAAVIASAIALILI
                     IAIEYISHTISTRMVIAALVGIFIGLIFAHLLVIAFKSLPLTMQPNTVNIISAIIYHI
                     IGFGSVLFFIINCEDIAIFDAIVPVKRDEARDGASYKILDTSVIIDGRISDICDTGFI
                     EGILVIPNFVLNELQMIADSSDSIKRNRGRRGLDILNKMQKDQRILVKISDVDFPEVS
                     EVDAKLVKLAKLMTAKIITNDFNLNKVAEFHGVKVLNINQLSNALRPIVLPGEEMSVS
                     LIKEGKDPNQAIGYLDDGTMVVVENGRSRLNSTVDVIVTSVLQTTAGRMIFARIKED"
     gene            complement(17701..18180)
                     /locus_tag="PLJ75_03010"
     CDS             complement(17701..18180)
                     /locus_tag="PLJ75_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPD04216.1"
                     /translation="MFKIGDKIVYPLHGVGIINAIEKKVVLNKRNEFYLITIINSGMK
                     VMIPTAKAESIGLRKVIPKKDVTKVLNLLRQTDIETEDDWKIRYQNNVDKIKSGSIFA
                     VAEVARDLYKRGKDKELSIMERKLYENAYQLMIHEIALAKDIDVDEAGNIVSDALSS"
     gene            complement(18341..20743)
                     /locus_tag="PLJ75_03015"
     CDS             complement(18341..20743)
                     /locus_tag="PLJ75_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPD04217.1"
                     /translation="MKERLKACLNSFKQFIIKYMHDTRTWFYGIIVVSLIGGLLFGYI
                     VAQVQNCLGIDNLKRFQPSVPTKLYDVNGELIAELFQEKRDLVSFEELPKTLIRAFVA
                     TEDQDFYTHIGISPMAMTRAMVKNIISLKIAQGGSTITQQLAKRLFTSGERTFTRKAL
                     ELVLTLQIEKKFSKDEILEMYFNQIYLGHGCYGIASAAELFFNKEVRFLSLAEGSVLA
                     ALPSAPGRYSPFMNTRNAYIKNWDILNRMVDCGYLTNEKAKEVYTSFWPIFVDTIKTE
                     FPTKTAYSKIVDNAPFFTDYVRQILISRYGKDVVYNEGLQVYTTLNLKRQQAGEKYLV
                     EGLTRQNEISARANQYLSGAVDRGLFSVYQQLGLLFNLPRIIVKSDTETIFKKYMVDN
                     LLNEMELLALLCNVDAMHNDIEKFMIVTSGISSSLKVEGALIAIEPKTGYITTMVGGS
                     EFAVSNQYNRSTQARRQPGSAFKPFVYGAGIESKLINTAMALPDAPIVDIDAQGSTWS
                     PNNYEGEFMGMVHLRRALAASINVISVRIYDIIGADTIIDFASRVLKVSPSRFHPGPS
                     LALGSSELTPLEMATGYAIYANRGRDVIPFSIRYITDRDGNEIENIEEDVGNIIAAKE
                     ADGSIQIIPENVNYIMTSLMQTVAEGGTPTEALRLIAKFDKKCAGKTGTTQNWTDAWF
                     CGFTPDIATVVWVGYDKPFMSLGRHQAGAAVAAPIWGFYMKEVYNGMPDPQWPAQPKD
                     VFYGATCKYSGCAPGPNCHELISDLMIPGGTPRCICNGIHYNMKSVFEQYMEKEGLKN
                     ED"
     gene            complement(20820..21029)
                     /locus_tag="PLJ75_03020"
     CDS             complement(20820..21029)
                     /locus_tag="PLJ75_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04218.1"
                     /translation="MNKYTHYILIIAGIVAVGYITFRYIIPLLFKLISFIVGFLAYGA
                     AVVLAVAIVLIIIGYIVRMYKTHSQ"
     gene            complement(21050..22195)
                     /locus_tag="PLJ75_03025"
     CDS             complement(21050..22195)
                     /locus_tag="PLJ75_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD04219.1"
                     /translation="MKRKILQIVPCILLIGVVFFVFNNFSCSNSEPSVFGNNSSSPLK
                     GHQETARAIEIQDAFRKIYELYKDRVVFITTEQVVELPPNPFFDDPFFRQFFGIPQQK
                     SRKQKRSGLGSGFVLSSDGYICTNYHVIANVDSVKVKVKNKEYEATIVGYDQRTDLAL
                     LKINPGDKLEPVFLGDSDQVKVGDWAIAIGNPFGLDRTFTVGVISATSRKDVDMMGGS
                     QSHIQTDAAINPGNSGGPLVNINGEVIGINRMIYSQSGGYMGIGFAIPINTAKSILEQ
                     LKKYKKVKTGYIGVQIVPLTEEYANELGLKNTEGALVGGIMENSPAEKAGIQPGDVII
                     SVDDKNIKSYEDLIEIVNKTPIGKTLKVGVWRNKQKIYLFVTVKERP"
     gene            complement(22348..23013)
                     /locus_tag="PLJ75_03030"
     CDS             complement(22348..23013)
                     /locus_tag="PLJ75_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04220.1"
                     /translation="MNKLIIIPEDKLIAYCLNLSHPALESVVIRECVDPLCNGDNPSL
                     YRRHFSLFHALYTIKTCPQYFHLMVYIHPMDIVIRSYPTNNACIHYDPWCDAFCNRET
                     VDGIYCSFHYEFYEPYNHNCLYDPLMKFYCDSYNINYEFSVELNKVYHGFKKYIASLK
                     EVEKALFFFQFNAIDTCNRSLLQKRYRELVKKYHPDRYNGDMAMIKQINEHYSLLKEI
                     VVI"
     gene            complement(23006..23203)
                     /locus_tag="PLJ75_03035"
     CDS             complement(23006..23203)
                     /locus_tag="PLJ75_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04221.1"
                     /translation="MKKIVICLIISILFFGVSCKKNDDNKVPMEQIQIDGSHDFSIIF
                     KDIIEKEKKDASRYKKGPFDE"
     gene            23359..23949
                     /locus_tag="PLJ75_03040"
     CDS             23359..23949
                     /locus_tag="PLJ75_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04222.1"
                     /translation="MRISNDLPVPSMMVPSTGRKTGVPVAQVNNVQHHVSLQKTISAY
                     TVVQEAHKLVTMALNISFELIQKAYSKESSYTEIIAKLATIDSYFNRLSPGTANVPAI
                     PPADVVAGSSQEITQLQKAVANNDIKTMNAIHNSLINNSQILESYKQTLSRALGVADN
                     DSPDIKVIKKIIEDNASNAFMVHQPLHHDNVKKLLS"
     gene            23977..24381
                     /locus_tag="PLJ75_03045"
     CDS             23977..24381
                     /locus_tag="PLJ75_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04223.1"
                     /translation="MELFDGFIAAIASEFKMKFNKTSEGAYTTTIEFENNRSQEILIT
                     LNKDESGDRIINYYSVIGTIKNESFELYKFSLKLNSTFDYGALALLNDTLILRNSLLL
                     KDCDPQRFMKSLVYIAAKADELEEMLITRNIY"
     gene            complement(24378..24902)
                     /locus_tag="PLJ75_03050"
     CDS             complement(24378..24902)
                     /locus_tag="PLJ75_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04224.1"
                     /translation="MVINKIPAILSLILFMTIEIVLPGMVYSDDTTTFNTLRISSEDI
                     PAGFMFGEIPQFAKKVFPENPCIVSTEGIQFLAGKLYPEGNPYNIKNMYVAIMSESSR
                     PFGDDIVCYVLMFKDQTSASSEIKKIQEFYKYNKDRIVLIIKGQIAIMLFADDINNYK
                     YIAELGNKLSSKLD"
     gene            25181..27268
                     /locus_tag="PLJ75_03055"
     CDS             25181..27268
                     /locus_tag="PLJ75_03055"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPD04225.1"
                     /translation="MSDVITTTPQFIRKRDGRIVPFDIEKITEAIFKAAKAVGGTDRN
                     AAVHIAESVVDILSILYKDEHIPTVENVQDLVEKMLIEKGHAKVAKAYILYREQHRKI
                     RESKDLLNEGIQLIEDYLDRSDWRVNENSNMSYSLQGLNNHIASAITAKYWLERIYPP
                     EIRDAHTNCDLHLHDLGLLSAYCVGWDLRDLLLTGFGGVPGKVESRPAKHFRSALGQI
                     VNFFYTLQGESAGAQAFANFDTYLAPFIRYDNLDYAGVKQCLQEFIFNMNIPTRVGFQ
                     TPFTNVTMDLTVPRSIANEHVIIGGESMDAIYAEFQKEMDIFNTAFAECMFEGDARHR
                     IFTFPIPTYNITKDFDWDNPSIHLLWEITAKYGIPYFANFVNSDMDPDDARSMCCRLR
                     LDNRELRKRGGGLFGANPLTGSIGVVTINMPRLGYLSKSKDEFLASLSHLMDVASNSL
                     EIKRKVLENFTSNNLYPYTRFYLRDVYKRFKKYWINHFSTIGLVGMNEACLNLLGQDI
                     TTDEGKAFTGEVLDFMRSKIVMYQHATGNLYNLEATPAEGVSYRFARHDKNRFSDIIT
                     SGKNEPYYTNSIHPPVGYAHDIFDLLDHQDQLQAKFTGGTVVHCFIGEKIDDPEMVKI
                     LVKSIAYNYHIPYFTITPTFSICPVHGYIPGNHTYCPYPHTKEELEMFGIEVEIEDRE
                     LSKPIETTHRIVS"
     gene            27640..27831
                     /gene="nrdD"
                     /locus_tag="PLJ75_03060"
     CDS             27640..27831
                     /gene="nrdD"
                     /locus_tag="PLJ75_03060"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPD04226.1"
                     /translation="MQRNTEKKIPVEVYSRVVGYFRPVNQWNKGKQEEFHERKEFMAK
                     ELLKVVDHEYSGYTENITH"
     gene            27794..28489
                     /locus_tag="PLJ75_03065"
     CDS             27794..28489
                     /locus_tag="PLJ75_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPD04227.1"
                     /translation="MNIRGIQKTSLIDYPGAISTVLFTGGCNLRCSYCHNPSLAYNDC
                     NLHYYSNEEVIDLLRSRKHLIGAVVLTGGEPTIAKGILQFLENIKSLGMKIKLDTNGF
                     QPDILKKIIIKNLVDYVAIDIKTSPQKYSLLTKTKLSFNCIKESIRLLKDFDIQFEVR
                     TTCVPEFVTLEDLHSIKEQIGQVSHYYLQQFNNDHTMDEDLHRVKPYPEEILLLFRQF
                     VQTFATYCDIRGL"
     gene            complement(28892..31564)
                     /locus_tag="PLJ75_03070"
     CDS             complement(28892..31564)
                     /locus_tag="PLJ75_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04228.1"
                     /translation="MVKYCITIVLSLYIVAIVTVDASSDIIGHDTVTMYCSGDSQALA
                     EQYVNGAQNLTIPNLLILADSEHIQQIIGIEKTIELLELLSTNAQSPYEKMFINDIAD
                     ISKERFYKNDVKGNQRWYIYDNSKEPMPKSVISFQTQLDTLILPDKNKIAVMQSCAGY
                     IPVKQYTQILHATGLCSASFELHKPVSMYVFSNMEYAIFINGTKVCVNTTTTVKKDAR
                     IIHIEPQEGFSIAIYYKPDNDMYLKILFRDETNATMQLPATDTMYYNPVNCYEIFYEY
                     QNQLLSNYMSNHSPDNALQLALFSESIHSTEAFKYYHQALRSHNDFISYRLCSLLLRY
                     SRAFPDSILLLRTNVQKNIQPQTVLGGYFYDMLYSPESMNIAEIQWLPILIQTLYIQK
                     EELINKKADLDFLFKRFPQSHELAFTIASIYSSYDNHKATTILETYKNLNDKELTLLI
                     HCYTENKQFEKIVTILSGVREHTFFYDYINALIALGKYGEAKSALFKTIAQGFDTEAY
                     ALLATIAQLEGYDASMYSQKHHAIVNSLVVHPDYMKATFDDYVQNYFFKQLAYEIINH
                     TTNKLMYSCYAVRMVHDALYCTIYDAYHCTDISVFNTYQFSPDTENISYTLYAYNNEG
                     KMKSYTFYPGNYTIQQKIQEYLHDSHCEFFVIAISFRLRKNIDIMHIQQNYKQSPFTL
                     DIIVLSPESDVTISGTMRLNEITTNVRYIHHYTCNSNELLSPNNREFVIMAIQPQALI
                     TWIEEQNALFKRGIELLDIKEIQGNSIDEAIHEVLQELQTYTIADNVYGITSIVQWTL
                     LHKGTQLDAMLYARYLLAKKGIISCIALGDSHKKIVAKKNNLYFDTVFLYIPLTPDKG
                     IWLTYKNSYPSSEVLNISNILLIVGGEIISKQSN"
     gene            complement(31575..32936)
                     /gene="mnmE"
                     /locus_tag="PLJ75_03075"
     CDS             complement(31575..32936)
                     /gene="mnmE"
                     /locus_tag="PLJ75_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPD04229.1"
                     /translation="MQETICAPATPPVHAPIAIIRISGPESLRVASSIFKQHNKQTIS
                     TPRYAYYGTIFDGEYEIDDAIVIYYAQPHSYTGEDMVEIFCHGNPIIVNKIMNRLLAL
                     HVRIAQPGEFTKRAFFNGKMDLTEAEAINHIITARSEWEIATAIQQKHGSLRTTINKL
                     KQTIVELKADIECAIDFIDQDIEIISYQDAQQRIEDIIHTIQEIINRCKVGNNLSHGI
                     DIAIVGKPNAGKSSFLNCVVNHERAIVSDEPGTTRDVIKESVTIHGIQCNLYDTAGIH
                     DVASGIELLGVQKSYRIIDEVSLLFFILDATSGFTVEDSHILERISSKPYIPVINKCD
                     IASPEQINAINTQLPKKGIVFAATTGYGMDVLEHTVSDFIQSSYVNYTNSFIADIRII
                     QLLEQSSAGAHEVREQLLKQSPHEIIASALQDVIDTIGAITGEITVDDVLNSIFSRFC
                     IGK"
     gene            complement(32938..33579)
                     /gene="jag"
                     /locus_tag="PLJ75_03080"
     CDS             complement(32938..33579)
                     /gene="jag"
                     /locus_tag="PLJ75_03080"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPD04230.1"
                     /translation="MKVLDIEGKTVEEATKKALLQLKIDDMNKINIEVLDEGKSGIFG
                     FGISRPAKIRVYYQQQNDVGEKAKEIIEKIMSLMDVNATVKDFKESENKVYVELESLN
                     SGLIIGKKGKTLEALQFMINLLVNKVTSSEKKIILDIESYRAKRERALRKLSKEIALK
                     VARTGKPWTLEPMNPFERRLIHLTLQNDSKVTTKSEGQGIYRKVKIMPIEKGR"
     gene            complement(33736..35355)
                     /gene="yidC"
                     /locus_tag="PLJ75_03085"
     CDS             complement(33736..35355)
                     /gene="yidC"
                     /locus_tag="PLJ75_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPD04231.1"
                     /translation="MEKRALIAVLLSLLIWILWFYLFPPQEKKPAVQDKKVEVEQTVE
                     IKKPELKQLSKFLELSRLKEEVTVDSHYYKAVLSNHGAACTSFIDKTRNIELVVNKSK
                     YIARGIFDFPVHFTTGEFLLSTESDYTNWKLQKETDSIRFFTGAIVQNNPLHIVKTFT
                     FDEKGQFFTVSFSITNRAINEFSFPEGRLIYSPSDFLGPSMDFNNSYNELNSIYYLDD
                     DFEKARKGSGFFSNHEDIKEINGVVKWAGIMSRYYLLIMIPQGFQGSGVVYDNREHTG
                     FRTGIIVPAKPLKPGEKIEHVFKIYVGPKNKDKLLAVDKQIGDAADVSKWIEPIRDFM
                     MWCLLGINKLLGNLGWSLVIFSVITKIMFLPLTKRSTESMKKMQELNPVVQELREKYK
                     DKPDILNKKIMEVYKKNKVNPMGGCLPLLLQMPFFFALYSALINSVDLWQAPFILWIK
                     DLSLPDTVFTISGFNINILPIVMTATTYLQQKMSTGEASQQQKMLLFMPLIFIVIFWN
                     MPSGLVLYWIMQNVLQIAHQMVVNRKPKTEK"
     gene            complement(35466..35687)
                     /gene="yidD"
                     /locus_tag="PLJ75_03090"
     CDS             complement(35466..35687)
                     /gene="yidD"
                     /locus_tag="PLJ75_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014951464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPD04232.1"
                     /translation="MKINIISLPILLIKMYKLIISPLLPNVCRFYPTCSSYAIEALSK
                     HGLCKGGFLSIKRILRCHPYCDGGYDPVP"
     gene            complement(35684..36049)
                     /gene="rnpA"
                     /locus_tag="PLJ75_03095"
     CDS             complement(35684..36049)
                     /gene="rnpA"
                     /locus_tag="PLJ75_03095"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPD04233.1"
                     /translation="MKRRYSIKGRRSFKDVIVNGRRFYSKGIRMIVHIQPAHETLFIG
                     NESVSHFSIAVNKNFGDAVERNKIRRRIKSILNTLLPQIQQGFAVIIFPDKTCKAMPY
                     THLSGLVKQLLLRSGVLKQ"
     gene            complement(36075..36233)
                     /gene="rpmH"
                     /locus_tag="PLJ75_03100"
     CDS             complement(36075..36233)
                     /gene="rpmH"
                     /locus_tag="PLJ75_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014934381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPD04234.1"
                     /translation="MKRTFQPSVIKRLRTHGFRERMSTKAGREILKRRRQKGRCKLTV
                     SDEKRNVK"
     gene            complement(36432..37235)
                     /locus_tag="PLJ75_03105"
     CDS             complement(36432..37235)
                     /locus_tag="PLJ75_03105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04235.1"
                     /translation="MIPLLCPICKDSRSKQIANDIFLCKPCKILFNNAYQPSPYTADY
                     FGEDYKNQYGKTYEDDHDAIYTASLLRIEKIKKLLNNNLSEKSLLDIGCALGFFCKAA
                     KDSGFQFVEGIEISEYAAQYCKEKFSISVTTVDFEKFLTAKKYDVITAWYVMEHFANP
                     FAMFQKVYSMLHNDGIFACTLPSYFGPLFYCHRDIWIKNHPPDHRIDVSPRGITRLLK
                     NMGFVKVKCYPSGYHPERFCSLASKHKKIYIKLSDSICFSDTIMVIAKK"
     gene            complement(37241..37729)
                     /locus_tag="PLJ75_03110"
     CDS             complement(37241..37729)
                     /locus_tag="PLJ75_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HPD04236.1"
                     /translation="MNQVKKIEKILESALNEDLTSYFLLIKAMRKHLPLFGSTKRITA
                     ALLKECIGDISKLDLSEITSLNMAKSDGTIVFEDIKNGKARIFINIGKNHKITPGDLI
                     REISKRSGIDGKLVGKIDIHSTYSFIEIPEQYAELVLVSLDKARIKGIPIVVEPAKKK
                     KQ"
     gene            complement(37822..38469)
                     /locus_tag="PLJ75_03115"
     CDS             complement(37822..38469)
                     /locus_tag="PLJ75_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPD04237.1"
                     /translation="MKIKTALITRSKELVSEVRLVQPLDASIHGSINTLKDEGFDIIL
                     FDSEQIDISDAQFYLSKIRKRFSHVPVILIVRTGYLESINRDWFFDDFIIFPFRKGEL
                     KVRIDCLIGSEIEIKDAVIRVGNIAIEPDKYIVSVNNEKVSLTFKEFELLKLFIENKG
                     VVFTRQELLSRIWGIEYIGGTRTVDVHIRRLRSKIGDEFNNIIETVRNVGYKCKD"
     gene            complement(38459..39634)
                     /locus_tag="PLJ75_03120"
     CDS             complement(38459..39634)
                     /locus_tag="PLJ75_03120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD04238.1"
                     /translation="MELHKKINILIINYEFPPLGGGGGVATYDLALEWVKVANVHIIT
                     SSFKGLPPYEYINGIHVHRVKILFRKSRDAATFISMFSYLPAGFIRALSLFKKYKFDI
                     INTHFVVPSGPLGYVLSKLFKIPNVLSLHGGDIYDPSKKFSPHRNRFFKLIVQYLLNK
                     ADAIIAQSSNTRNNAFHYYKIKKDIGIIPLAFHPPVIPKSSRKLLGLHEKDFICITIG
                     RLIKRKAIDVLIKAIARINNQHVKLIIMGDGPEREYLEQLVQELALENIVTFLGFVSD
                     DKKYAYLSVSDLFVLTSLHEGFGIVFMEAMYCGLPIVTTNSGGQVDFLENEHNALLID
                     VGDIQKCAECIMRFKNDKKLYAQCSKNNKARITAYYAENVAKQYMDIFYQLVDMNEN"
     gene            40527..41546
                     /locus_tag="PLJ75_03125"
     CDS             40527..41546
                     /locus_tag="PLJ75_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPD04239.1"
                     /translation="MSIKDILIYFYLPVIVFTVLCFCAIVVGIIYWIKKYKELLIILF
                     YFLIHAGMTAMYIYPFNVFAFSLQYAFFLFCLLMFCFSAVFMVLPDASNNSDSSYRAV
                     QCIVGKSILVYVSIVFLTPKEQLIISTVDCMVSLVFISVKPKPATSCVQPFAEKEKFI
                     FIIVITIVFLWISIAKIFLFHVLILGMTILVIVNVVLVCVIALKVYVYKDMDFIAIQQ
                     ENYCGMYEKHFKIVQIIKEHINEYYTHPIDKYTIALQCEMNPDYLCKIFKSNEGITIN
                     EYIKNIRLKKALETLSNTDRKIIEIALDVGYANLATFYRHFTQFYKISPNQIRIKKSN
                     NITTV"
     gene            41747..42076
                     /locus_tag="PLJ75_03130"
     CDS             41747..42076
                     /locus_tag="PLJ75_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04240.1"
                     /translation="MIELADKGNGTFVVKINMQEVHTLDVPDLKEKLQQAIVNKGIKR
                     LVVDLADVKMITSSGIGIFLNINQNLKSQLRLAAPTPEVQKVLELTKVTSMIKVFNSV
                     DAALQSF"
     regulatory      42219..42319
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            42395..43555
                     /locus_tag="PLJ75_03135"
     CDS             42395..43555
                     /locus_tag="PLJ75_03135"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009180908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HPD04241.1"
                     /translation="MKAEIIPGNSAGIVTTNYFTFAEPPDELLLVSGKKLGPITLAYE
                     TYGTLNSDKSNAILILHALSGSAHAAGYHSKKDIYPGWWDIYIGPGKTFDTSHYFVIC
                     SNVIGGCSGSTGPSSINPETGKEYGLDFPIVTIQDMVNAQYHLIKFLGIQKLLSVVGG
                     SMGGMQALQWSISYPEMVQSVVAIATAASLSAQGIAFNEVGRQAIMLDPNWNYGKYYN
                     NENPKNGLSLARMIGHITYLSEKLMHEKFGRRLQHSDIFKFHLDLEFEVESYLHHKGN
                     DFVQRFDANSYLYITKAIDYFDLKNDFNGDLANAFVNVNSNYLVISFSSDWLYPTSQS
                     REIVRALRMNSKNVIFTEIQSDKGHDTFLLPHEQLEHNIANFLKREFEKVHR"
     gene            43555..44178
                     /gene="metW"
                     /locus_tag="PLJ75_03140"
     CDS             43555..44178
                     /gene="metW"
                     /locus_tag="PLJ75_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HPD04242.1"
                     /translation="MKEYRVAYDLILKEIPYGARVLDLGCGDGQLLKLLQTTRKVKGF
                     GVEISPEGVSQCVEKGLYCYQGDIDEGLNDYKDNSFDYVIVNQTLQSTKKPEIVIRES
                     LRISLNAIFSFPNFAHYSLRLYISIYGRMPKNKLLPYEWYETPNIHLLTIKDFRDFCN
                     AFGYPIKKEMHFSTIRSHSVTIPIMPNLFAEYGFFVLDGGPFTSDTI"
     gene            44806..46695
                     /locus_tag="PLJ75_03145"
     CDS             44806..46695
                     /locus_tag="PLJ75_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPD04243.1"
                     /translation="MKIQFVQLPLIDHGLQYITGNIPNAAATLHAFCELTSGGTMKGL
                     QLPYSIQNYGSDSMIVNHILHNEPTHVAFTCYLWNVERSLAIAKTIKAMASHIIILFG
                     GPEIQVNSYILKQHQPCIDIFVIGTGEFFFNHYYNNTCQQFIHMVNGNAVFIQGSDTF
                     IPLSQIVEPYTSGYLTPMHDKSLSVEIIRGCPFKCTYCLYSKNTSRVRALPPHKLADI
                     IKKSMSDEIQELYLLAPTFNQSSHFNDYLQTLISSRCSVSLHTELRADTITSNIAMTL
                     KKANFRSAEVGIQTLNKKCLQKIGRKTNPEKELQGIFYLKDAGINLQVGIIPGLPYDT
                     PESFYTTMDTLLDAGLGNELELYPLMVLPGTALYDEAIAAGATFLQKPPYYLIEGFNF
                     TQHDITAITCYFEDTTGFTFDTPYLPSFIFQQGELVSGACIDLTKRRTFPGEVFDYMQ
                     TSHVTFYLLCDSYPQIEHAVELIIQQYPWDTMLCSIVLMYDAVLNDEDIASKVFTATH
                     NTFYCRMMYFQDTYDKLPVRIFQVFESLKTYLRAKDHYRCINPIIKLLRSNYDEITQY
                     HFAYPVPAVVAKGCAGIYGQWIKEQYAECIDIVSFEDDDDQNMIYKTLGIDGKPTIPF
                     TTLSI"
     gene            complement(46692..46979)
                     /locus_tag="PLJ75_03150"
     CDS             complement(46692..46979)
                     /locus_tag="PLJ75_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04244.1"
                     /translation="MVMLFIVYHNAVDEEVVEITKKYAKGYTKFVGVLGEGNKEPQLG
                     THIWGGLNNCLMVAIEDNKMKDAIASDIASLKVKFKDAGVSIFVLPLKEML"
     gene            complement(46992..>47372)
                     /locus_tag="PLJ75_03155"
     CDS             complement(46992..>47372)
                     /locus_tag="PLJ75_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPD04245.1"
                     /translation="LTGSQFSVATLLGIIMLVGIVVNNAIVLVDYTDILIQRGWGLRE
                     ALVEAGTKRLRPIMMTTLTTVLSLIPMAFSGGDGSEMSQPIGRAVMGGMSTGFIFTLI
                     FIPVIYMIVETRREKYSAKHKAKF"
BASE COUNT        15388 a         7756 c         8495 g        15633 t
ORIGIN      
        1 gttgttacta taatgaattg taaggaaaac tttttcatta aattttttac tactgtaata
       61 tgatcgccaa taacagtgca gataattgct ttaggttttg cctctttaaa tttatgctca
      121 agcgcttcag ctggttccag ggcactacat ggtaaatgga ttaaccctgc acgcgatatt
      181 gcataatcac atataataaa ttctgttgaa gtgggaagta ttgttgctat gatatcattt
      241 ttttttagtc caaacgcatg gaatgcagat gtcagcttat taacatatat taataattcg
      301 ggatatgata aaaagaaatt ttcctgtata agtccatttt ttctatattg ttttgctgct
      361 atctcaagta tatcataaac cggtttgtcg ggatatggtt taaaggtttt tggtatacct
      421 aaaattttat attctctata ccacggtagt gcttccattg cttacctcca taagacataa
      481 aaataaaaaa atatctcaac cacattgatg tatgtggttg agataaacaa tcatttatca
      541 agtgaatagg ggttgggttg ccatagcaaa gctcatgtct ccttcaatct tcattgcgcc
      601 tgacatgaaa agatttactg gattggtttc ctttttcatc aacgctgcag aatcttttgt
      661 actcatacga aaaatagact ggggggatgt ggcaccattg agaattgctt caatagtaaa
      721 ttctgaaccg tcatcatttt ttagtttagc tatgaagctg ccctttaatg atgatagtgc
      781 attgtaacgg gcacgtgtca gatctttctg catgcccagc aacatatcaa gattattggt
      841 gtcgatcatc tttttaagat cagcttcaga aatttttaat ctcagcatag cattagggat
      901 atccccacaa ggtgtgatat ctttaccatc gttaaccttg aatccatacg actttccatc
      961 tacttcaact accatagtaa tttctgtgcc atttaattct tttggtgcat tggaattttg
     1021 aagcattccc aaagcttctt taggcataat ttcggttaaa aattgttcaa ccgtggtatt
     1081 ggatgtaata gccataatca tcctcctact ataaaaaatt taaaacgtac aggaaataaa
     1141 aatttttctt gaaatactga catgtcagta ctataatgtt ttgtcaagat tttttctaaa
     1201 aattttctaa aaatttttag tgctggatag ttattctata ataattttgc tttgcttact
     1261 atccataata agtgtaatgg tacacaatgt aataagtttt ggaggtaacc aatgtcaagg
     1321 gctgatgaaa cgcgagataa aattttattg tatgctaaaa aattattttc tgaaaaagga
     1381 tattattcat cacagatttc tgatattgtt gaatctgcta agattggcag aggaactgtc
     1441 taccagtatt tttcaaataa ggagcacctc tttatatcat tacttgaaag atttttgatt
     1501 gaatgggaag aatatattaa agaactatca gtcagtgagg atctaagtat tatttcacat
     1561 tacacctatt taaaaaggcg tttgtataag gcatttaaat ttttttttag cgacagagat
     1621 agagcaaata ttattttaag ggcggggatt ggtttgcccg agcattttga acaaaaaata
     1681 caacattttg agaataagct gataaacatg attattgctg atctggcaat agctgttgaa
     1741 tttaaccata tagacatgaa cgaagattta agttttgcag ctaatcttgt agctggtggt
     1801 gtattccgat tggctcatta ctatcttttg attgaatcca atgatgaggt actgttacag
     1861 aatttagatg acattagcag caaagcagca cattttattg gaaatggtat ttttagtaaa
     1921 aaatagttga ctaattttta tggttgataa agtactgaca tgtcagtact attattatgg
     1981 gaggtaagta tggtagaaga gattagtttt gctctatctg aggaacaaca gatgatgcag
     2041 gatactgtac ggcagttggt gcaggctacc atcgcagacg gtatccatca gatggatgaa
     2101 aataggcagc tggacaaaca ggctgttcaa aaattctggg aattgggcat tattcaatca
     2161 atgattccag aagaatttgg cggttatggg atggatagtt cacctgtatt gcatacgctt
     2221 gtattggaag aattggctta tggggatatg acatttgcaa tatatgcaat gcttcctgct
     2281 gtttttgttt tgccaatagt acatttagga gcaaaagaac aaaaagaact atatcttcca
     2341 gaattttgta atgcaacatt tgttcctgct acattggcta tcaatgaatt aagtgtagcc
     2401 tttgatgtat ggaactgtgc aactactgct gaaacgaaag gggacaaata catcatcaat
     2461 ggcagcaaat gctttgtgcc gctggctgac gaatcaaact ttatgattgt cgtggctcaa
     2521 tgtgagggta acccggagct ctttatagtt accagagata actctggaat aaaagttgga
     2581 gaacgggaaa aaaattgtgg atggtgggca ttgccaactt ttgaagttac ttttgaaaat
     2641 tgtatcattc ctgcatcaca tagattaggt gatagcaccg ctattacatg gttgctgcaa
     2701 cgaacaagaa cagcccttag tgcaattggt acaggtattt cacgagcatc gtatgaatat
     2761 gcaaagaaat atgcaaaaga gcgtcagcaa tttggtgaac caatagccag ccgacaggca
     2821 attgcattta tgatagctga aatggcgtat gaggtagatg ctatgcgact gttatcatgg
     2881 aaggctgctt caaccattga aaacaattta gatgcgacaa ggttatcata tttagcaaag
     2941 atgtatgcag gtgaaatgac catgaagatt actgactatg gtgtccagat acttggaggc
     3001 catggttata ttcgcgacca tcctgttgag cggtattatc gcaatggtag aagcatagca
     3061 ctatgtgaag gtatggcaat tatataaacc agtggaggta tatgtatgat tcaattagtt
     3121 acacctgaat atttagtaac attacaaaac aatatgcgca aattggctga aggagtattg
     3181 cgccctatat cacgaaagta tgatgaagct gaacatgaat atccaaaaga acttgatatg
     3241 ttcaggaata ttaaagttat ggctcgtccc aagaaaaaaa aagacggtga aaaatcatca
     3301 tctggtgaag aaaaaaaacc aaaacgaggt gcaaatttag gaatggtagc aactattgaa
     3361 gagatgtgct ggggtgatgc agcgctgcta ttatcgttcc ccaatgctgg tttgggcaat
     3421 gcagctatca ttgcagttgc taatgatgaa caatttgaac gattcggaaa taaatgggcc
     3481 gcgatggcaa ttaccgagcc aggtgctggt tctgattcgg gtgccattac cactacagca
     3541 actcgggatg gtgacttctg ggtattgaac ggtgaaaaga tatttgtaac ctgtgctgat
     3601 agaagtgagg tggtagttgt ttgggcgaca gttgataaaa aggctggcaa atcaggtatt
     3661 aaatcatttg tagtagagaa aggaactcca ggatttacat tagaacattt agagcataaa
     3721 ttgggtatca gagcctcaga taccggaacc tttgttttaa gcgattgtag aataccctat
     3781 aacaacatat taggcgatcc agaagtaaag atgtccactg aaggctttaa aggagtgatg
     3841 aaaacctttg ataatacccg accaatggtt gctgctatgg ctaccggtat agcacgagca
     3901 gcagtagaat ttactaaaga acaatttgaa caaaagggat acacatttga ttataagaaa
     3961 aatactgcaa atatttccgc agttgaacgt gaattgtata tgatggaggc aaatgtagaa
     4021 gcaatgcgtc ttttaacatg gcgtgctgca tggatggctg ataatggtca atttaataat
     4081 cttgaagctt caatggcaaa agcaaaagcc ggacggtatg caacactggt tacgcagcgc
     4141 tgctgtgagc ttttagggcc tgttggctat tcaactcact atttagctga aaagtggatg
     4201 agagacagca aaattatgga tatatttgag ggtacgggac aaattcagca tcttattgtt
     4261 gcccgcaatg tactggaaat gtcaagtaaa gagttaaagt aatatagatt tataatcatt
     4321 atcattattt cataggaggt gtttatgaat actatcgatg atgtaaagaa cattgcagtt
     4381 ataggctcag gtgctatggg acatggtata gcacaggttt gtgcccaaaa ttgctttccg
     4441 gttaccatga ttgatattaa acaagagttt ttagatactg cattaaaaaa agttaaggaa
     4501 agtttagatt ttttagaatc aaaaggaaaa ctgaacggca cagttgatga ggtgatgaaa
     4561 aaaataaatg ttacaactga tatgaaaaaa ggggtgtctg atgcgcaggt tatcattgaa
     4621 gctgtacctg aaaatatgaa gcttaaacaa gaggtatttt cacaagcttc tgctgcatgt
     4681 gctgctgatg caatattagc caccaatact tcaacgatga gtattacaga tattgcaact
     4741 gcagtaaata atcctgagcg atttgccggg atgcattttt ttaatccagt aacaagaatg
     4801 aaattagttg aattgatcta tggtgcaaaa acatcagaaa aaactatcga tatattgtat
     4861 gaacttgcca aaaagattga taaaattcct gtgagggttt taaaagatag gccgggattt
     4921 attgttaata gaatcaatgc accaaatcag gcactactga gcgctatcct tgatgaaggg
     4981 aaaattcatc ctgcacagat tgatggtaaa atgaagaaga tgggtgttaa aatggcgcct
     5041 tttgaaacag cagattttgt tggacttgat gtattttgtc atactctgga atattatgca
     5101 cagacactat cgccacagta taagcctgga aaatttttaa tggaatgctt atccaaggga
     5161 aatttaggaa tgaaaagtgg caaaggtatt tatgaatgga aaaatggaag agcggttata
     5221 cctgaaatgc ctgatacaga agagattact ccgctgcacc ttatggctgt acaggtgaat
     5281 gaagcagtca aagtatttaa ggaaggaatt gccgccaatg ttgctgatat tgatgatggt
     5341 gttaaatacg gaatgaatgc atttgctggt ccttttgctt tagcatctgg ggtgcaacct
     5401 caacaattga cagattgcct gaattatctg tcgcaacgat ataaacttga cattttaaag
     5461 ccagaacctg aaataattga tggttcattt aaaactatag gaagatagga ggtatgctat
     5521 gtcagatgca gtattattag aacaaaaagg aatggttgca attttaacta tcaacaaacc
     5581 aaaagcaaac cagttgagcc atgatgtttt tgaaacaatg cggaaatatc ttgataccct
     5641 ggaaattgat aacaacatcc gcgtgcttgt tattactggt tcgggtgata aaatattttg
     5701 tgctggtgct gatctatcgc agggctttgg tgatttcagt gcagttgatt ttttaaagcg
     5761 tggtcaggat atatggaata agatagagga ctatcctaaa ccagtcattg ctgcaataaa
     5821 tggtcatgca ttgggaggcg gatgtgaact gtcaatggca tgccacatac gaataatgaa
     5881 aaaaggtgct cgtattggtt taacggaaac aaatctgggg ataatgcctg ggtatggtgg
     5941 gacactccgt ttgccgcgcc ttgttggacg tgctaaagcc ctggaaatga tactttttgg
     6001 aaaacagtta gaggcagatg aagctctggc tttaggtttg gtacacagag tatatgatga
     6061 aggatatatg gatgaggtga tgaaatttgc tgaagaacta actaatcgtc ctccattgtc
     6121 aattaaggca atattaaaag ttatgtctgc cggtccttct atgtcacctg aaatgcactt
     6181 aaagatggaa agagaagaat tagctaaatt atttaccaca aaagattgta tggaagggat
     6241 gatggctttt gctcaaaagc gaaaaccaga attcaaaggc gaatagataa agagctataa
     6301 agaacctcct gcttatgaaa acctcacatt gttttgtgag gtttttttct tgtttttaga
     6361 attgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttttt tgttaaactt
     6481 tgaggagtct acaatggctg gtacaggtgt tagagaaaat ggcaaacaaa gaagattaat
     6541 tatggaaaag tctcaggttc tcttctggga aaaggggtat gccgaaacaa gtatgaaaga
     6601 tattgctaat gaatgtggtt tcagacctgc aaatatatac aatttttttg caagtaaaga
     6661 agagatactt ttttttattt taaaagaaga gatggaagaa attgttaatc ctatacggtt
     6721 tctggaatat gaagaaaatg atcctttaca acaattacgc tttctaatag aaatccacgt
     6781 aaaaattact ttaggggaga aaagagcttc caaattatta tttgatacag aactcaggaa
     6841 tctttcagca gaaagaagaa aagtaatatt agatttacgc gatgattata acagaatagg
     6901 atattcaata ctaaaaagag gaattaataa agggttgttt agagccatcg atgaaaagat
     6961 tgcgttaaat ctgattgctt ctatgatagt gagaacacgc ttatggtttt cccctgaaga
     7021 ttggatgaca attgaagatt tggtagaatt tatttttcaa tttgctctta aagggctttc
     7081 aaagtagtta taattttttt atgggaaaaa aagaatgtat acgttgtggt gcatgttgtt
     7141 ttgttgatat ggttgcttat gcaacaccag aagatttttt acgctgggaa aaagaaaaaa
     7201 gatatgatat acttcatata atagagaatg aaaacagtat atgggcagga gatcatttaa
     7261 tatcaacgcg tgatggacat ttaattggac gatgtccttt tttgataatg gaaaacaatg
     7321 ggttatatag ctgcatgatt tatgatacaa gacctgctgt atgcaaggat tttgaacctg
     7381 gtgtttctat aatgtgccca ctgtttgcat taaaagaata ataaattgcc aattaataac
     7441 tgagcaggat aaaaaaatga ataaaaactg ggctgtttcc ataattatag taaatggatt
     7501 aattttttta gttcaattgt tattacgtaa tgtgtttgtt ccatatgata ttgccggaaa
     7561 tgttgtgtca attgatattg taacgtatta tctggggcta attcctcaat tggtagttga
     7621 aaaattatat atatggcaaa ttatttctta tatgtttttg catagcacat caagttttgc
     7681 tcatatcctt tttaatatgt atgcggtttt tatttttggt atgcctgttg aacgattgtg
     7741 gggtagtaaa aaatttataa tatatttttt aatatgtggt ataggggctg gcgtagcgat
     7801 ctttacgata gctctcataa ctggtggtat gggatattat atacctacaa tcggggcatc
     7861 aggtgctgtt tttggattac ttttagcatt tggcgtgtta ttccctaatg ttgagatatt
     7921 actctttttc attattccga ttagagcaag gacattggtc atactttatg gaggattgga
     7981 attattttta gaactatcag ggggggcaga caatatttca catattggcc atttaggagg
     8041 gcttgcaaca ggtattctgt attttattgt tactcgcaag catgctatcc gctataaaac
     8101 aaaaggattt gttgcagcac agatgaaaaa agaaactatt cccgataaac cggatgttaa
     8161 ttataaaagg gaagcagaac tacaaagggc aatagtacaa aaaatcaggg agcatggagt
     8221 tgaaagttta aatgacgatg aagttcaata tatacgctat aaagagataa tgattgatat
     8281 agaggaagag gaggtaccct gtgatttaca aatattttta gatgacaatt gtaacgattg
     8341 tgagaacaga gacgcatgtt ttataaaaat tgtgaaatca tttaagaaag aatagaatac
     8401 accaataaaa tgattttgat aatgtaaaat taatctttac aacaaaattg tagtatacta
     8461 acaatgtaca agttttaaaa aattaatatt tatataaaga ttcttcttgc tggataatgt
     8521 aaaaaatatg attatcattt ttgtaatgat aataacaaaa ttttattaga agggagaatt
     8581 gcaatgacaa aacgcgtata tctgtttggc ggtaatgtta ctgaaggtac tgctgacatg
     8641 aaaaacttat tgggtggtaa gggcgcaaac ctggcagaaa tggcaagaat tggaataccg
     8701 gtgccggcag gctttacaat tactacagaa gtatgtaatg aatattataa aaacaatcaa
     8761 aaattgacgg aaagtttaaa agaagaggtt acagaagcat tgcgtcaggt tgaagggata
     8821 atgggtgcta agtttggcga tcattccaat ccattattgg tttcggttcg ttcaggtgct
     8881 cgtgcttcaa tgcctggaat gatggatact attttaaatc tgggtattaa tgaggatgtc
     8941 ataaaaggtc ttgtgaaaaa gacaggaaat gaacgttttg catgggattc ataccgcaga
     9001 tttatccaaa tgtatggtga tgttgtttta ggtcttaaac cagaaagtaa agatgaacat
     9061 gatccatttg aagttataat ggatgaatta aaagaaaaaa gaaatgtaaa aaatgatctt
     9121 gatctaactg ttgaagattt aaaagagctt gtagcaagct ttaaaaaact tatacaggaa
     9181 aaacttaaag tgaccttccc tgatgatcct tatgaacaac tctggggagc tataggcgct
     9241 gtattccgat catggaacaa tgaacgtgca cgactctatc ggaagatgaa taatattgat
     9301 gatagctggg gtactgctgt caatgtgcag gcaatggtgt ttggtaatat gggaaatgat
     9361 tgtgcaacag gtgttgcatt tacgaggaat ccagcaacag gagcaaaaga attttttggc
     9421 gaatatttaa ttaacgctca aggtgaggat gtggttgctg gtatcagaac accgttacag
     9481 ataacactgg aagggtcgcg acgttgggca cgagaaaata acatctctga agatgaacgt
     9541 aagaataaat atccttcact tgaggagtac atgcctgaag catataatga gcttgttaat
     9601 gtatataaga ccctcgaaaa acattatcgt gatatgcaag atatagaatt taccatacaa
     9661 aataaaaaat tgtggatgct gcaaacacgt aatggcaaaa gaacagctcg cgcggctatc
     9721 aatattgcag ttgatatggt tgaagaaggt ttaattacaa aagacgaagc agtactaaga
     9781 gttgatcctc aatcattaga tcagctatta catccaacat ttgatccaaa agctcagcgt
     9841 aaagtgattg ctaaaggtct acctgcgtca ccaggggcgg caacagggaa gattgtattc
     9901 aatgctgaag atgcacatga ttggaagggg cgtggtgaaa aggttatttt ggtacgaata
     9961 gaaacatctc cggaggatct taagggaatg aatgctgcag aagggatttt aacagcacga
    10021 ggtggcatga cttctcatgc tgcagttgta gctagaggta tgggtaaatg ctgtgtatca
    10081 gggtgtggaa tgctggatat agattatact aaacggcaga taaaagttga tactacaatt
    10141 cttaaagaag gagattatat ctcaattgat gggtcaacag gggaagtgat gcttggaaaa
    10201 gttgctacta tggaacctga agtaacagga aaatttggta ctattatgca gtgggcggat
    10261 gaaataagga aattaaaggt aagaacaaat gcagatactc cgcatgatgc cagggtggca
    10321 cgaaattttg gggctgaagg aattgggctt tgtcgtacgg agcatatgtt ctttgaaggt
    10381 gatagaataa aagctgttcg tgaaatgata ttggccccta cctatgaagg cagaaaaaaa
    10441 gctcttgata aattattgcc aatacaacgt gaagattttt atggtatatt taaagctatg
    10501 gaaggtcttg gcgtaacaat acgtttactt gatccaccac tacatgagtt tttacctcag
    10561 gatatagaaa cacagaaaga gatggcaaaa gagttaggtg ttactgttga tgatgtaaaa
    10621 gcaaaaattg aagaacttca tgaatttaat ccaatgttag gacatagagg gtgccgtttg
    10681 gggataacat tccctgaaat cactgaaatg caatcacgtg caatatttga agcagcgtgc
    10741 cagctggtaa aagaaggcat tgatgttaaa ccggaagtta tggttccttt agttggacat
    10801 gttaatgaac ttatcatgca aaaggaaata ataataaaaa cggctgaagt agttatgaaa
    10861 gaaatgggag taacagttaa atacatggtt ggtacaatga tagaagttcc acgagctgct
    10921 ataacagccg atgagatagc aaaagttgct gaattttttt cgtttggaac taatgactta
    10981 acccagatga cctttggatt tagccgtgat gatgctggaa aatttttaaa agaatatgtt
    11041 gataagaaga tattaccaga tgatccattc agggttattg atcgcgatgg tgtaggacag
    11101 ttaataaaaa tggcatgtga aaaaggtcgt tcagtgaatc caaacctaaa attaggtatt
    11161 tgcggggagc atggtggtga tccaagttcc gttgagtttt gtcatgtggt tggtctggat
    11221 tatgtaagct gttcaccttt tagagttccc attgcacggt tagctgcagc tcatgctgct
    11281 atccgttttc caaaatgatg tttgtagaga acgtctatct atgaattata atagatagac
    11341 gttctattag tttgcaggag gttcagataa ctatgcgatt attaattgtg ataattttaa
    11401 cacttagtag tattgcaccg gcatatgctc aatatgataa ggctgtaaag caatttgagc
    11461 agggtaatta tgatgcggcg ttagctgaga tagcatctaa tcttgatgta tcacaagatt
    11521 ttgaaccaaa ttccctcaat tataatttgc gttatttagc tggacatatt catgcaaaaa
    11581 aaggtaacta tgaatccgct ttagcacatt taaaacggtg tgctgaaatt aaaaaagata
    11641 ccatcgatcc aattattgat attgctttta tatatattga taaaaaaagt tatactgatg
    11701 ctattacatg ggcaaggaaa gctattcaaa taaaacaatc agctatagct tattatatat
    11761 taggattatc gttttcaggg attcaatcgt attggcaagc aaaagagtat ttagaaaaag
    11821 ctattgcaat tgatcctgag atgtattatg catataatga attagggaat gtattaatgc
    11881 atctgggtaa attaagtcag gctcaaactg cttattctgc tgcgtatgca ctttatccct
    11941 cttcagcata tatatgtaat aatttagcac tgtgttatgc taaatcagga gatatcaaaa
    12001 aagctaaaga aatgattggg aaagcacata cattcgatgc atcaaataca atcataacgg
    12061 caaattacaa tagaattatt aacgtagcga acaaataatt tacgttgcaa gtgtattacg
    12121 gtgtttatca aatgtaatta ataacaggtt aggtacttaa gtattttcta tatcgtattc
    12181 ctgatagtga attaataaat aactatactt atttatatcc tgctttttgt atccatccta
    12241 ttatataaca aaaagaaaga gcaattatca gttatagaaa gcatatccag cagaaacatg
    12301 aaaaagtata gaatcaataa cagcactttt tttgttataa aaaatttgat gagtttgtgg
    12361 attttctgca ttaggggtag aagaaaatgt tatattatta cgaacccatt ctcccccaag
    12421 ttgtataaca aggccaccac taaaaaatat gcttaacagt aaatcaacac cataggttaa
    12481 gctggtaaat tgttctgaag gtgggccgtc ataaccatcc agtagttctt ttgctatagg
    12541 agtgggatta tattgcgaaa atgctatacc aattaaagga gacagagcta tatcccatgg
    12601 tttccgagat gtcatatgaa aagagggacc taaatagata gagtaatcac tatataatgt
    12661 ttttttccat gatttatagt cagaaccaaa atttgtgttt tgtattatta tagaccttct
    12721 taatttagaa cggataccta tataatctgc aggcatatat tcaccagaaa ttgttattcc
    12781 aaatgccaga tgggaaagat agataagctt tgaggtttcg ggtgtaagtt gatacgatga
    12841 tttttttgtg aatttttctt ctgctgttat aagattgcca taaggcatgc caattgaaac
    12901 agcaccaagt ataagaagct gatccttatg catcccttcg gggggtttat ccaattcatc
    12961 aaattcggat gaaaataaat tggaaaatgg tacagctata aataaaaaaa acaatgaata
    13021 aaaaaaagtt attaataaat atttcattta tttttatcat tttccataat gagtagtatt
    13081 tttttaacca gttgctttgc tatatcatca tattgtgaac tttcaactaa tatatcattg
    13141 gtgtcccaga ttaatgtaag tccacaacag tattgtgctc tatagcgcca atcccatgct
    13201 atgcctggtt ccttacgcac tgtaagaaga atctcgccat tttcagtatt aatagctttg
    13261 agagtaaatg tatcaataca gttaggttca tatttatttt tacgatatgc ctggagtgct
    13321 gtacctcgac cgattattat tacatcagca ccaaataatt ttcctaattt tatagcctgt
    13381 ttttcgtcaa ttaatccgga agcctgcaat tgctgctcct gcaatatttt ttcaattgct
    13441 tctctttcaa ttgttgctac cttgccactt ttaaaaaatt gatgggagat agcatcggca
    13501 aattcattgc cccaatcagc tccggttatc tcaaagggta aaactgcaac acgcttcatt
    13561 cttttttgaa tgatctgttt gttggtatat gctacatcta tgctggtaca tgaaaataaa
    13621 gaaattaaaa gtattaagct aataaattgt aattttttca taatagaacc ttttgaatta
    13681 tttaaataag tttataaatt atttaattat accatttgaa tataaatttt ggcaaatatt
    13741 attttattag attattaatt attaatatgc tatttacttg attccttttt attgtaagat
    13801 tcagtgataa aaccgattat aaatatgtat agtataataa aaaaatacaa ataatgaaag
    13861 gattatgata gatttgagta ttagaataca atgccatatt ttacaatata ttagagttgc
    13921 aaccattata cttcttataa ctttaatttc gtcttgtact aataggcaaa taactaacaa
    13981 ttataacaat ccagttgtat cttttttttc acattatgtg tgcgatatgc aaaatgcaac
    14041 taaaattatt atcaaatatg aacttatttc caatttgact catctgctta taatggataa
    14101 tggtgggaat aaatattact tactggaaca tgaacatatt accgaaatgc tcaataatgt
    14161 tacccataag ctttatgatg atcttataat tactaataat gcctatcagg ttgtttactc
    14221 gatgcaaaat aaaaattatt atggacgcag aatatttgat atatcgcctc aattacagca
    14281 atgtttaatg aaatgcaatt acaatgatat atctatctgt gaaccatctg aatatcctgt
    14341 gatgtcagga aattttaact tttgtgttgt ttatcctatt acgtctaacg aaagaaataa
    14401 aggttttctt atcggattta ttaataaaat caagctttat gatgttttac cagaagatgc
    14461 aataattgtg gataaatacg gaatggttat aataaaacct gagaagagta ttttaaaaac
    14521 tatacccata cctgatttac aaaataatta tgttacattt gaacaaaagc aatatgcaat
    14581 aattgagatt aaatataaaa atattgaatg gtatttgtta tctccgtaac ggtcactgta
    14641 tattttttat aatatccttg atggtatata tgttttcagt attatcgata tcaatatttg
    14701 ctttaaccag agctcttgca atgtttatta aacaattatc gtttaaggtt ttaatatcat
    14761 tcattaacat catatcaata ataccaggca aaagaagata tgagttttct atattaagat
    14821 attcgtttgc tttaacacct ctttcttcag atattaacat atcaaaatga atggctggat
    14881 tagacaatac taattgtcct ttctgaagct tataataatc gatattttca caaatatctg
    14941 atacaaacaa aagtatatct gcattttcaa agacattatc agtgctggtt gctataccgc
    15001 tttccttttc aaaatagtaa aggttttttt cattaggatc aaaacctaat actttgctac
    15061 acccaatact gtttaacaat cgagtacatc gaagtaaaga tgttgttaca ccaatgaatc
    15121 caacaacgga atctgataat ttgatgttat gcaacttcaa tagattcatt atttttacag
    15181 taatataaag tggtatctca tcaaattctc tattaataat agggggtgtt acctcactca
    15241 tctgatggaa taatgatata tcttctatgt tattcataat ggcaacacgc attaaggcat
    15301 aattgcctga aacagaataa agtgttttta taatatcttc ttttgattta tatttcaaca
    15361 aaaggggttg aggattataa ttattgcaat ggctaataat gagtgaatca cgttcgatca
    15421 ggagataatt atatatttca tctggttgca attcattttt ttcttcattt aggataatca
    15481 tcccaatatt ttttgatata cctgtgtatc gataggtatc ttttgttatg gtattatctt
    15541 taatcaactc aaaatatccc agaactttgc cctcaaattc atctggagtt tcaaatggta
    15601 tcttgcttga gactttaagc agaccaccat ctatgccttg attcataatg tcactgcatt
    15661 ctatgagtgt gcattctttc gttatccatt gtaatatttt ttctttaaat aatcttctat
    15721 cagtataaac aagttctgct ttatatattt tatagttttg ttcagatgat aaataattaa
    15781 tgctttgaat ggtacaatct gcttggatga ctttttgaat aaataataaa aaatttttat
    15841 catcacatcg gaaggttaga gtaatatata atttcatatt tcctccaaaa aaaagcaatc
    15901 attatcattg ttttatcggg aaaaaattgc taataataaa catttatcta ctttcagtaa
    15961 agctaaaccc atcaaagtgt ccttataaaa gggaatgcat aactactact atctttgtaa
    16021 tactgtatgt atataatgga ttgtttgttc tacctttttc ataatatcgg cattttctat
    16081 atcatttttg ctatttatgt ttggtaataa taagaagttg tcctcatcat agtgatactg
    16141 caatgaattg aaataatgtc gtaatgtaat tttccccggc ataaacatat cataataatt
    16201 cccaccacca attaatatag ccattgcatg ctgttttttt tgtgatggat tattcttata
    16261 gtattcccag aaaacctggc agcggtcgat aatcgctttt agaggtgaag gaagatgcga
    16321 aaaatacaat ggtgatgcaa caatcattaa tgtactttgt tgtatggtag cgtaaagcat
    16381 atccatatcg tcgtggaaaa tacagccaaa ttcattatgg caaaatccac agtctataca
    16441 tggatgtaca tgtagttcat aggcaaaatg tattgctttt gttatgttcg atagttcctt
    16501 aaaaagtaat tgcaaaattg caacagtatt tccttcttta tgaggacttc cgcaaagaac
    16561 aaccacatca taattagtct tctttgattc ttgcaaagat cattctccct gcggttgttt
    16621 ggagtactga agtaactatc acatcgaccg ttgaatttaa ccgtgatctc ccattttcca
    16681 ctactaccat agtcccgtca tcaagatatc caattgcttg attggggtct ttcccttctt
    16741 ttattaatga aacagacatt tcttcacccg gtagcactat tggcctgagg gcgtttgaaa
    16801 gctggttaat atttaaaact ttaacgccat ggaactcagc aactttgttt agattgaaat
    16861 cattggtaat aatttttgca gtcataagct tggctaattt gaccaatttt gcatcaactt
    16921 ctgaaacttc gggaaaatca acatcagaaa tttttactaa aattctctga tccttctgca
    16981 ttttattgag tatgtctaaa cctcgtcttc ctctattacg cttaatagaa tcagatgaat
    17041 cagctatcat ttgcaattca ttcaatacaa aattgggaat cacaagaata ccttcaataa
    17101 atccagtatc acagatatct gaaatacgac catctataat gacgctggta tcaagaattt
    17161 tatatgatgc gccatccctt gcttcgtcac gttttacagg aactatcgca tcaaaaattg
    17221 caatatcctc acaatttatt ataaaaaata atacggatcc aaatcctata atgtggtaaa
    17281 taatggcact tataatgttt accgtgtttg gttgcatggt aaggggtaat gatttaaatg
    17341 ctatcactaa aagatgcgca aagatgagtc ctataaaaat gccaaccagc gctgcaataa
    17401 ccatccttgt tgagatagta tgtgaaatat attctattgc aataatgagt ataagtgcta
    17461 tagcagatgc aatcacagca gctattatgg ctgattgcaa tgaaatttgg aaaaaatata
    17521 aaccggtaac aacagctgaa gtaataataa ataaaaaacg tataattgct atcatggata
    17581 tactgactcc tttatatata ttaaaataaa attttgataa aaaaaacatt atggatccat
    17641 aaagttaaat attttataaa taaagtgact gatacaaaaa ttataaactg tagtgagcaa
    17701 ttatgaggaa agtgcatctg atacaatgtt gcctgcttca tcaacatcaa tgtcctttgc
    17761 taaagctatc tcatgtatca tgagctgata agcattctcg taaagctttc gctccattat
    17821 agaaagctct ttatcttttc cacgtttata tagatccctg gcaacctcag ccactgcaaa
    17881 tattgatcca cttttaatct tatcaacatt gttttgataa cgaattttcc agtcatcctc
    17941 ggtctcaatg tctgtttggc gtaacagatt taatactttt gttacatcct tttttggaat
    18001 aaccttcctc aaccctatgg actctgcctt tgcggtagga atcataacct tcatgccact
    18061 gttgatgatt gttataagat aaaattcatt ccttttgttt aatactacct ttttttcgat
    18121 agcgtttatg atacctaccc catgtaaagg atacactatc ttatcaccta ttttaaacat
    18181 aggataccct caaaataaat taacccacat ttattataac gtataaatta ataattttag
    18241 cacataattt taataaaatc aagtagaaaa ttttttttaa gaaagtatta tcggctagta
    18301 atagagtttg ttatttgatt gtgaaatttg acaatagcta ttaatcctca ttttttaaac
    18361 cttctttttc catatattgt tcaaaaacag atttcatatt atagtgtatt ccattacaaa
    18421 tgcaacgagg agttccgcca ggtatcatta aatcactgat taattcatga caatttggac
    18481 caggtgcaca gcctgaatat ttacaggtag caccataaaa aacatctttt ggttgagctg
    18541 gccattgagg atccggcatt ccattgtaaa cttccttcat gtaaaatccc catatgggcg
    18601 ctgctactgc tgcaccagcc tgatgtcgtc ccaatgacat aaaaggttta tcatatccaa
    18661 cccaaacaac tgttgcaata tcaggagtaa aaccacaaaa ccatgcatct gtccagtttt
    18721 gagtagtgcc tgttttgcct gcacattttt tgtcaaattt tgcaattaag cgcaaagcct
    18781 cagtaggtgt acctccttcg gcaacagtct gcattaatga tgtcattata taatttacat
    18841 tttcgggaat aatctgaatt gaaccgtctg cttcttttgc tgcaatgata ttgccaacat
    18901 cttcttcaat attttctatt tcgttgccat ctctatcggt aatataacga atggaaaatg
    18961 gaatgacatc cctccctctg ttagcataaa tcgcatatcc tgttgccatt tctaatggtg
    19021 ttaattctga acttcctaaa gctaatgatg gcccgggatg gaagcgtgaa ggagaaacct
    19081 ttaatacgcg tgatgcaaaa tctatgatag tatctgcacc tatgatatca tatatccgta
    19141 ctgaaattac attgattgat gcggcaagag ccctccttaa atgaaccatt cccataaatt
    19201 cgccttcata gttattcggg ctccaggtgc ttccctgagc atcaatatca acaataggtg
    19261 catctggaag agccatggct gtattaatta attttgactc aatacctgca ccataaacaa
    19321 atggtttaaa tgctgatcca ggctgacggc gagcctgagt tgatctgtta tattgattgc
    19381 tcacagcaaa ttctgatcca ccaaccattg tcgttatata tccagtttta ggttcaatag
    19441 ctataagagc cccttcaact tttaatgaag aagaaatccc actggttact atcataaatt
    19501 tttcaatatc attatgcatt gcatcaacat tgcataataa cgctaaaagc tccatttcat
    19561 tcaacagatt atcaaccata tattttttga atattgtttc tgtatcactt ttgacgataa
    19621 ttctgggtaa attaaaaaga agtcctaatt gctggtatac agagaataat cctctatcaa
    19681 cagctcctga aagatattga ttggcacgag cagatatctc attttgcctg gttaaacctt
    19741 ccacaagata tttttcacct gcttgttgtc gtttcaagtt taatgtggta tatacttgca
    19801 atccttcatt atacactaca tccttgccat atcgggaaat aagtatctga cgtacataat
    19861 ctgtaaaaaa tggggcatta tccacgattt ttgaatatgc ggttttagta ggaaattcag
    19921 tttttatggt atcaacaaaa atgggccaga aagaggtata tacttcttta gctttttcat
    19981 tagttaaata gccgcagtca accattctgt ttaaaatatc ccaattttta atataagcat
    20041 tacgggtatt cataaatggg gagtaccgac caggagctga aggtaatgca gctaaaacgc
    20101 ttccttcggc aaggctaaga aatctaacct ctttattgaa gaaaagctca gcagcagatg
    20161 caatcccata acatccgtgt cctaaataaa tttggttaaa gtacatttca agaatttcat
    20221 ctttggagaa tttcttttca atttgcaatg ttaaaactaa ttctaatgct ttacgggtaa
    20281 atgttctttc gcctgacgta aaaagccgtt ttgccaattg ctgggtaatg gttgagccgc
    20341 cctgtgcaat tttaagagaa ataatatttt taaccattgc ccgtgtcatt gccataggac
    20401 ttattcctat atgcgtatag aagtcctgat cctcagttgc aacaaacgcc cttatcaatg
    20461 tttttggaag ttcttcaaat gatactaaat cacgtttttc ttgaaaaagt tcggcaataa
    20521 gttcaccgtt aacatcatac agtttagtag gaacacttgg ctggaaacgc tttagattat
    20581 ctataccaag acaattttgt acctgagcaa caatataccc aaaaagcaaa ccgccgatga
    20641 gtgatactac tataatcccg taaaaccatg tgcgtgtgtc atgcatgtat ttaattataa
    20701 actgtttaaa actgtttaag caggctttca aacgctcttt cacgttgttc tgacctcaat
    20761 tatttattat attactatag cttagtttag cattcaaatg gatagtggta tcaagttatt
    20821 tactgagaat gagttttgta catccgaaca atatagccaa taattattaa tactatcgct
    20881 acagctaaaa caactgctgc tccataagcc aaaaagccta ctataaagct tattaattta
    20941 aataatagcg ggataatata gcgaaaggtt atatatccca cagcaactat tcccgctatt
    21001 attaatatat aatgtgtgta cttattcata ctgtgcatgc ttttaatatc taaggccttt
    21061 cttttaccgt aacaaataag tagatttttt gtttattacg ccatacgcca acttttaagg
    21121 ttttgccaat aggtgtcttg ttaacaattt ctataagatc ttcatatgat ttgatatttt
    21181 tatcatcaac tgatataatg acgtcacctg gttgtatacc agctttttca gcagggctgt
    21241 tttccataat accaccaaca agagcacctt cagtattttt aagccccaat tcgtttgcat
    21301 attcctctgt caatggaact atctgtacgc caatatagcc ggttttaact tttttatact
    21361 tctttaactg ctctaaaata gacttagccg tattaatagg gattgcaaat ccaatcccca
    21421 tatagccacc actctgggaa taaatcatgc ggttaatgcc tataacctcc ccattaatat
    21481 taaccagagg cccaccagaa tttccggggt taattgctgc atcagtctgt atatgcgact
    21541 gtgaaccacc catcatatct acatcctttc ggcttgtagc actaataact ccaacagtaa
    21601 atgtacggtc caacccaaag ggatttccaa tagcaattgc ccaatctcca acctttactt
    21661 gatcagagtc acctaaaaaa acaggttcaa gcttatcgcc aggatttatt ttcagtaatg
    21721 ccaaatctgt tctctggtca tatccaacaa ttgtagcttc gtactcttta tttttaacct
    21781 ttacctttac tgaatcaaca ttcgcaatga catggtagtt agtacagata taaccatcgc
    21841 ttgatagaac aaaaccactc cccaatccgg aacgtttttg tttacgtgat ttttgttggg
    21901 gaatcccaaa aaattggcgg aaaaagggat catcaaaaaa agggtttgga ggcaactcaa
    21961 ccacctgttc ggttgtaata aaaactaccc tgtccttata tagttcatag attttcctga
    22021 aagcatcttg aatctcaata gcacgagctg tttcctgatg ccccttcaat ggtgaactgc
    22081 tattattgcc aaatactgaa ggttcgctat ttgaacagga aaaattatta aaaacaaaga
    22141 aaactacacc aatgagaagt atacatggaa ctatctgtaa tatttttctt ttcattagtt
    22201 atctcctaat tactataaat tattaaatca aaagttattg tataataggg acttaattta
    22261 ttcaataata tatggtaaaa taaaaattaa taaaaaatat acaccataat aatatatatt
    22321 atcattgtca atatataata gaaatattta tattacaacg atttctttca acaagctgta
    22381 gtgttcatta atctgtttaa tcatcgccat atcaccgtta tacctatcgg gatggtattt
    22441 ttttaccagt tccctgtaac gcttctgtaa caatgatctg ttacaggtat ctattgcatt
    22501 aaattgaaag aaaaataaag ccttctccac ctccttgaga ctggcaatat atttcttaaa
    22561 accatggtaa accttgttga gctcaactga aaattcataa ttaatattat atgaatcaca
    22621 ataaaatttc attaatggat catagaggca attatgattg tatggttcat aaaattcata
    22681 gtgaaacgag caatatattc catcaactgt ttctctatta caaaaggcat cgcaccaggg
    22741 atcataatga atacacgcat tgtttgtggg atagcttcgt ataacaatat ccataggatg
    22801 gatatatacc attaagtgaa aatattgtgg gcaggtctta atggtatata aagcatgaaa
    22861 cagtgaaaaa tgcctgcggt atagtgaggg attatctcca ttacatagag gatcaacaca
    22921 ttcgcgtata accactgatt ccagtgcagg gtgtgacaaa ttgaggcaat atgcaatcaa
    22981 tttgtcttca ggaataatta tgagcttatt catcaaaggg tcccttttta tatcggctgg
    23041 catctttttt ttctttctcg ataatatctt taaaaatgat agaaaaatca tgtgatccat
    23101 cgatctgtat ctgttccatg ggtactttgt tatcatcatt ttttttgcac gataccccaa
    23161 aaaacagaat agatattatt aaacaaatta ctatcttctt catagagtat aatccattat
    23221 ataaatatat tcttatattt acaaacatac aacacatgtt gtaatttttc aatattgatt
    23281 tttttgatct tcaaaatttt actgttgaca ttattaattc catatactac acttactggt
    23341 gatcagggag gggattacat gaggatatca aatgatttgc ctgtaccgtc aatgatggtc
    23401 ccatcaacag gcagaaaaac aggtgtgcct gttgcacaag taaataatgt acagcatcat
    23461 gtatcgttgc aaaaaacgat ttctgcatat acggttgtcc aggaagctca taagcttgtt
    23521 actatggctc ttaacatttc gtttgaactt atacaaaaag cttattctaa agaatccagt
    23581 tatacagaaa taattgctaa acttgctacc atagacagct attttaacag gttatcacct
    23641 ggtacagcaa atgttcctgc tatcccacct gctgatgttg ttgcaggatc atctcaagaa
    23701 attactcaac tacagaaagc ggttgctaac aatgatataa aaacaatgaa tgctatacat
    23761 aatagtttaa ttaataactc acaaatactt gaaagctata agcaaacact atcacgtgct
    23821 ttaggagttg ccgataacga tagtcctgat ataaaagtta ttaagaaaat tatagaagac
    23881 aatgcatcaa acgcttttat ggttcatcaa ccgttacatc atgacaatgt taaaaagttg
    23941 ttatcataat tattgattat tgtgaaaggg tggaatatgg agctttttga cggttttata
    24001 gcggcaatag catcagaatt taaaatgaag ttcaataaaa cttcagaggg tgcatataca
    24061 acgacgatag aatttgaaaa taaccgatct caggagatat taattactct taataaagat
    24121 gaatctggag atagaataat taattattat tctgtaattg ggacaatcaa gaatgaatct
    24181 tttgaattat ataaattttc attaaaactc aatagtactt ttgactatgg cgctttggca
    24241 ttattaaatg atacacttat tttacgtaat tctttattgc ttaaagattg cgaccctcag
    24301 cggtttatga aatcgcttgt ttatattgct gcaaaagctg atgaattaga ggagatgtta
    24361 attaccagaa atatttatta atcaagcttg cttgatagtt tgttacctaa ttctgcaata
    24421 tatttgtaat tatttatatc atcagcaaat aacataattg ctatctgccc ttttattatc
    24481 aaaactatcc tatccttatt atatttatag aattcctgaa ttttttttat ctcgcttgaa
    24541 gcagatgttt gatccttaaa catgagcaca taacatacta tatcatcacc aaatggccgt
    24601 gatgattctg acattattgc aacatacatg tttttgatat tatatggatt gccctcagga
    24661 taaagcttgc cagctaaaaa ctggatacct tcagtgctaa caatacaggg attttccggg
    24721 aatacctttt ttgcaaactg tggtatttcc ccaaacatga acccagcagg aatatcttca
    24781 gaagatattc gtaatgtatt aaaggtagtg gtgtcatctg aataaaccat gccaggtaat
    24841 actatttcta tagtcataaa gagtattaag ctcagtattg ctggaatttt atttataacc
    24901 atacttcata cctctttgta gttaatatag tgtaactatc ggtaatacca tctcaaagta
    24961 caatttaaat ttttgcgtaa tatttatact atagtgattt atataaatat atgtttagtg
    25021 tttcattttt ttttgttacg tgacataatt atgattgact tttttatttt attattctaa
    25081 cgtaatatac aaaaacatat cttatcaagc taataataac catcgcattg tgtgctatac
    25141 agtgatcgtg ttggtttatt tcttttttgg gagtctttat atgtcagatg taataacaac
    25201 aacccctcag tttataagga aacgtgatgg cagaatagtg ccatttgata ttgaaaagat
    25261 cacagaagca atctttaaag cagcaaaagc agttggtggt accgatagga atgcagcagt
    25321 acatattgcc gaatcagttg ttgatatatt atccatattg tataaggatg aacatatccc
    25381 gacggtagaa aatgttcagg atttagttga aaagatgctc attgagaaag ggcatgctaa
    25441 ggtagctaaa gcatatatcc tctaccgtga acagcacaga aagatacgag aaagtaaaga
    25501 ccttcttaat gaaggaatcc agctcattga agattatctt gataggtcag attggcgggt
    25561 aaatgagaat agtaatatga gttattctct tcagggatta aataaccata ttgcttctgc
    25621 aattactgca aaatactggc ttgaacgtat ttatccacct gagatacgcg atgctcatac
    25681 caattgtgat ctgcatcttc atgatttagg tttgctgtcg gcttactgtg taggctggga
    25741 cttgcgagat ttattattaa ctggttttgg aggagttccc ggaaaggttg aaagcaggcc
    25801 ggcaaaacat ttccgaagcg cattggggca gatagtcaat tttttttata cattacaggg
    25861 ggaatcagcc ggtgcacagg catttgcaaa ctttgatacg taccttgcgc catttatacg
    25921 gtatgataat ctggattatg caggtgttaa gcagtgtctc caggaattca tctttaatat
    25981 gaacatacca actcgtgtag gattccagac gccatttacc aatgttacca tggatctgac
    26041 cgtacctcgc tcaattgcca atgaacatgt cattattggt ggagaaagta tggatgcaat
    26101 ctatgctgaa tttcagaaag agatggatat atttaatact gcttttgcag aatgtatgtt
    26161 tgagggtgat gcgcgacacc gaatttttac atttcctata ccaacgtata acatcacaaa
    26221 agattttgat tgggataatc cttccataca tcttttatgg gagataaccg ctaaatatgg
    26281 tatcccatac tttgcaaact ttgtaaattc tgatatggat cctgatgatg cccgctcgat
    26341 gtgttgccga ttaaggcttg ataatcgtga actgcgaaaa cgaggtggtg ggctttttgg
    26401 agctaatcct ttaacgggaa gtataggtgt ggtaaccatt aatatgccaa ggttaggata
    26461 cctttctaaa tcaaaggatg aattccttgc aagtttatca catttgatgg atgtagcaag
    26521 caattcactt gagatcaaaa gaaaagtctt agaaaacttt acttctaaca atctttatcc
    26581 ctataccaga ttctatctcc gtgatgtata taaacgattt aagaagtact ggataaatca
    26641 tttttcaaca attggacttg ttggtatgaa tgaagcctgt ttaaaccttt tagggcagga
    26701 cattacaact gatgaaggaa aagctttcac cggggaagtg ctggatttta tgcgcagtaa
    26761 aatagtcatg tatcagcatg cgacaggcaa tttatataat cttgaagcta cccctgctga
    26821 aggcgttagc tataggtttg cacgacatga taaaaataga ttttcggaca ttattacctc
    26881 tggtaagaat gaaccatatt atacaaattc gatccatcca ccggtgggat atgctcacga
    26941 tatatttgat ctccttgacc atcaggatca gttacaagca aagtttaccg gcggtaccgt
    27001 tgttcactgt tttattggag agaagattga tgatccggaa atggtaaaaa tactggtaaa
    27061 atcaatagct tataactatc acataccata ttttaccatt acgccaacat ttagtatttg
    27121 ccctgttcat ggatatatac ctgggaatca cacatattgt ccttatccac atacaaaaga
    27181 agagcttgag atgtttggaa tagaagtcga aatagaagat agagaattat caaaacccat
    27241 agagacaacc catcggattg tctcgtaaac gtataagttg ttgaatagat tgtatttatg
    27301 agtattgctt gactgtatat ttattttcaa gggattgttg atggctgcat tgcagttatg
    27361 gtaagtgata aaaatggagt actattgtca ttctgaacaa agtgaagaat cttttcgttt
    27421 tatggaagaa tagattctct gggagcgcgt ctctcagaat gatagtgtgc agtgtgttat
    27481 cctgaataat attcttaact ggtttcagga tcatttaagg atataaggtt acaaatttgg
    27541 attcctgaaa aggtcaggat tgacaaaagc ggcaaaagag attcttgttc aagtacagaa
    27601 tgatattggc acataaaact atattcggag ggggttctga tgcaaagaaa tactgaaaag
    27661 aaaatacctg ttgaggtata ttctcgtgtt gttggttatt tccgacctgt gaatcaatgg
    27721 aataaaggca agcaggaaga gtttcatgaa agaaaggaat tcatggcaaa agaattatta
    27781 aaggttgtag atcatgaata ttcggggtat acagaaaaca tcactcattg attatccggg
    27841 tgctattagt acagtacttt ttaccggggg atgtaatttg cggtgtagtt attgtcataa
    27901 tccatcatta gcatataatg attgtaacct acactattat tcaaatgaag aagtaattga
    27961 tcttttacga agtagaaaac atctcattgg agcggtagta ctgacaggtg gtgaaccaac
    28021 gattgcaaaa gggatactgc aatttcttga aaatattaaa tcgctgggaa tgaaaataaa
    28081 attagatacc aacggtttcc agcctgatat tctaaaaaaa attataataa aaaatcttgt
    28141 tgattatgtt gcaatagata taaagacatc tcctcaaaaa tattcgcttt taacaaaaac
    28201 aaagttatcg tttaattgta tcaaagaatc tattcgtttg cttaaagatt ttgatatcca
    28261 atttgaggtg cgcactacct gtgtgccaga atttgtaaca ttagaagatt tacatagcat
    28321 taaagaacag attggtcagg tatcccatta ttatttacag caatttaaca atgatcacac
    28381 aatggatgaa gacttacaca gagtaaaacc ttatccagaa gaaatattat tattgttccg
    28441 ccaatttgtc caaacctttg ccacttactg tgacatcaga gggctataga aaatctgttg
    28501 acattattgt atgatgtttt tctgtttcat gtaatctggc atttagcaag taattgcgat
    28561 tttttttatc aattgggcaa gttaaatata atactataat aattactcat tataaacaat
    28621 aattgtcact ataaaatggg tatatcaagc ttaaaaattt tgctatagta taaagaaaag
    28681 actcattatc atgatacaat atacaatcga agtaaagaaa taaatttgtt gttaaaaatt
    28741 gtatttccct gataatggct acattaattt ttttaaagga gacctgaaaa tctatgagca
    28801 agcagtataa tccaaggatt aaacgaaaac gagctaaacg aagactcaaa agaaaaaaga
    28861 aactaatggc aaagaagata gggcgataaa tttagttgct ctgtttgctg ataatctcac
    28921 cacccacaat aagtaaaata tttgatatat tgagtacttc tgatgagggg tatgagtttt
    28981 tataagttaa ccatatcccc ttatcagggg ttaatggtat atacagaaac actgtatcaa
    29041 aataaagatt atttttttta gcaacaatct ttttgtgtga atcccccagc gcaatgcagg
    29101 atataatacc ttttttagct aataggtatc gtgcgtagag catagcatca agctgtgttc
    29161 ctttatgcag aagagtccat tgtactatag aagtgatacc ataaacatta tcggcaatag
    29221 tataggtttg tagttcctgg agcacctcgt gtattgcctc atctattgag ttgccctgaa
    29281 tttcttttat gtctaagagt tcaatgccgc gtttaaacag ggcattctgt tcttctatcc
    29341 atgtaattaa tgcttgtggt tggatagcca tgataacaaa ttccctattg tttggcgata
    29401 acaattcatt actattgcag gtatagtgat gaatatacct aacattggtt gttatttcat
    29461 tgaggcgcat tgttcccgat atggttacat ctgattcagg agataaaacg ataatatcca
    29521 gcgtaaatgg tgattgcttg taattttgtt gtatatgcat tatatcgatg ttttttcgca
    29581 atcgaaatga tatcgctatt acaaaaaatt cacagtgact atcgtgtaaa tattcctgta
    29641 tcttctgttg tattgtatag ttgccgggat aaaaggtgta tgatttcatt ttcccttcgt
    29701 tattgtatgc atagagagta taactaatat tctcagtgtc gggagaaaat tggtaggtat
    29761 tgaatacgga tatatcagta caatggtatg cgtcgtatat ggtacaatat agtgcatcat
    29821 gaaccatccg cactgcataa catgaataca ttaatttgtt tgtggtatga ttgataattt
    29881 cataagcaag ctgtttaaaa aaataatttt gcacgtaatc atcaaaagtg gctttcatat
    29941 aatcgggatg cacaacaaga ctattgacaa tcgcatgatg cttttgacta tacatgctgg
    30001 cgtcatagcc ttctaattga gctattgtgg ctaatagcgc ataagcttca gtgtcaaaac
    30061 cctgtgcaat tgttttaaac aaagcgctct ttgcttcacc atacttacca agtgcaatga
    30121 gggcattgat ataatcataa aaaaatgtat gttccctgac accagaaagg atagttacta
    30181 ttttttcaaa ttgtttattt tcagtatagc agtgaatcag caatgtaagc tccttatcat
    30241 tgaggttttt atatgtttcc aatattgttg tcgccttgtg attatcatag gatgaataga
    30301 tagatgctat ggtaaaggca agttcatggc tttgcggaaa gcgtttgaac aaaaaatcta
    30361 aatctgcttt tttattaatg agttcttctt tttggatgta taaagtttgt ataagtatgg
    30421 gtagccactg tatttctgca atattcatgg actctggtga atagagcata tcgtaaaaat
    30481 agccacccag cactgtttga ggttgtatat ttttttgcac gttagttctc aacaacaaaa
    30541 tgctatccgg gaatgcacgg ctatacctca gtagcaatga acacaatcgg tatgagataa
    30601 aatcgttgtg tgaacgcaat gcttgatgat aatatttgaa agcctctgtg ctgtgaattg
    30661 attctgaaaa aagagcaagt tgcagtgcat tatcaggcga atggttgctc atatagtttg
    30721 acagaagttg attttgatat tcatagaata tctcataaca gttaacagga ttataataca
    30781 tggtgtcggt agcaggtaac tgcatcgttg cattggtttc atcacgaaac agtattttta
    30841 agtacatatc attgtctggt ttgtaatata ttgctatgct gaatccctcc tgtggttcaa
    30901 tatgaatgat tcgagcatct ttttttactg ttgttgtcgt attaacacat acttttgttc
    30961 cattgataaa aatggcatac tccatattgg aaaaaacata catagaaacc ggtttatgta
    31021 attcaaacga tgcgctgcat agtcctgttg cgtgtaaaat ctgggtgtat tgctttacag
    31081 gtatataccc cgcgcatgat tgcattactg ctattttgtt tttatcagga agtattaggg
    31141 tatccagttg tgtttggaaa gatatcacgc ttttgggcat tggttcctta ctattatcat
    31201 agatatacca gcgttgattt ccttttacat cgtttttata gaaacgttct tttgatatat
    31261 ctgcaatatc gttgataaac attttttcgt atggtgattg tgcattggta gaaagcaatt
    31321 ccagcagttc tattgttttt tcaattccaa ttatctgctg tatatgttca ctatccgcta
    31381 aaatgagcag gtttggtatt gtaaggtttt gcgcaccgtt tacatactgt tcagcgagtg
    31441 cttgtgaatc tccactacaa tacatagtaa cggtatcatg acctatgata tctgaagatg
    31501 catcaacagt aactatcgcc actatatata aacttaacac tatagttata caatatttta
    31561 ccattaccat gccatcattt gcctatacaa aagcgtgaaa agattgaatt gagaacatca
    31621 tcaacagtaa tttcaccggt tattgcacca atagtgtcaa tgacatcctg caatgctgat
    31681 gctataatct cgtgagggct ttgcttgagt agctgctccc gcacctcatg ggcacctgcg
    31741 ctggattgct ctaaaagctg tataatacga atatcagcaa taaatgaatt ggtgtagttg
    31801 acatacgagc tttgaataaa atcagacacc gtatgctcca gcacatccat accatagcct
    31861 gttgtggctg caaacacaat cccttttttg ggcaattgtg tattgatagc attgatttgt
    31921 tcgggtgagg caatatcgca tttattaata acggggatat atggtttgga actgatgcgc
    31981 tcaagaatat gggaatcttc aacggtaaaa ccacttgtag catcaaggat aaaaaataag
    32041 agtgaaacct cgtcaataat cctgtagctt ttttgaactc ccagcagttc aatccctgat
    32101 gctacatcat gaatccctgc tgtgtcgtat agattacatt gaatgccatg aatggtaact
    32161 gattctttta taacatcacg cgttgtaccc ggttcatctg aaacaattgc acgctcatga
    32221 ttgaccacac agtttaaaaa gcttgatttc cctgcattgg gtttgccaac aatagcaata
    32281 tctatcccat gagataaatt attcccaacc ttacatctgt tgataatttc ctgtatggta
    32341 tgaataatat cttctatcct ttgctgagca tcctgataag agattatttc gatatcctga
    32401 tcaataaagt caatggcaca ttcaatatca gcttttaact ctactattgt ttgtttcagt
    32461 ttattgatag tagtgcgtaa cgatccatgt ttttgctgta ttgccgtagc tatttcccat
    32521 tcactccggg ctgttataat atgattgata gcttcagctt cggtgaggtc catttttccg
    32581 ttaaaaaaag ctcgtttagt aaattctcct ggctgggcta tacgtacatg caatgccaga
    32641 aggcgattca ttattttatt aacaataata ggattgccat gacagaatat ttcaaccatg
    32701 tcctcgcctg tatagctatg cggttgtgcg taataaataa ctatcgcatc atcaatttca
    32761 tattccccat caaagattgt cccatagtat gcataacggg gtgtggatat agtttgttta
    32821 ttgtgttgtt taaaaataga agaagcgacc cgtaaggatt cagggccgct gattcgtata
    32881 attgctattg gtgcatgtac aggcggtgtt gctggagcac aaatggtttc ttgcatctta
    32941 cctgcctttt tcaatgggca taatttttac ctttcgatat atgccctgtc cctcactttt
    33001 tgttgtcact tttgagtcat tttgtaatgt taaatgaata agccttcgtt caaacggatt
    33061 catgggttca agtgtccatg gttttccggt tcgggctact tttaaagcta tctccttcga
    33121 taattttcgt aaggctcgtt ctctttttgc tcggtacgat tctatgtcaa ggataatctt
    33181 tttttctgaa ctggtaacct tattaaccag aagatttatc atgaattgca atgcttccag
    33241 tgttttacct tttttaccta ttattaaacc tgaatttaaa ctttctaact ctacatatac
    33301 cttattttcg ctttctttaa aatctttaac agtagcatta acatccatta atgacattat
    33361 tttttctatt atttcttttg ctttttcacc aacatcattt tgctgctgat agtatactct
    33421 aatcttagca gggcgtgata tgccaaaacc aaatattccg cttttacctt catcaaggac
    33481 ttcaatgttg attttgttca tatcatcaat ttttaattgt aataatgctt tttttgttgc
    33541 ttcttcaacg gtttttcctt caatatctaa aaccttcatc tccacgcctc ctatgatact
    33601 tataataatg aattatgata atagtatttg ttacacacct cctgaaaaaa tcaaaaaatg
    33661 gtagaaatct catattgtat ttaatgtact ataatttttg cattaagact tacatttatc
    33721 ctcgtttcca tgttgctact tttcagtctt tggtttccgg tttactacca tttgatgagc
    33781 tatttgtaaa acattctgca tgatccagta taataccaat ccggatggca tattccagaa
    33841 tataactata aatataagtg gcataaacaa aagcattttt tgctgctgag aagcttcgcc
    33901 agtagacatt ttttgttgta aatatgttgt tgctgtcatc actataggta aaatattaat
    33961 gttgaatcct gaaatggtga aaacagtatc aggtaatgaa agatccttaa tccacaaaat
    34021 aaatggtgcc tgccataaat ctacagaatt aatcagagcg ctataaagtg caaagaaaaa
    34081 tggcatttgc aacaatagtg gtaagcaccc acccatgggg ttgaccttgt tctttttata
    34141 tacttccatt atcttcttat tcaagatatc aggcttatct ttatactttt cgcgaagttc
    34201 ttgaacaact gggtttaatt cctgcatctt cttcattgac tcagtagaac gtttggttaa
    34261 gggtaagaac attatttttg ttataactga aaatattact aatgaccagc ctaaattccc
    34321 taacaattta ttaatgccaa gcaaacacca catcataaaa tcacgtatag gctctatcca
    34381 tttgcttaca tcggctgcat ctccaatctg tttatccaca gctaaaagtt tatccttatt
    34441 tttaggccca acatatatct tgaaaacgtg ttctattttt tcgccaggct ttagaggttt
    34501 tgccggtaca ataatccccg tcctgaatcc agtatgttcc ctgttatcgt aaacaacacc
    34561 agaaccctga aaaccttgcg gtatcattat gagcagatag tacctgctca tgatgcctgc
    34621 ccattttaca acaccattga tctcttttat atcctcatgg tttgaaaaaa atcctgagcc
    34681 tttccgtgcc ttttcaaaat catcatcaag ataatatata ctgttcaact cgttgtacga
    34741 attattaaaa tccattgagg ggcctaaaaa atctgaagga gaataaatta atctgccttc
    34801 aggaaaagaa aattcattta tagctctatt ggtgatactg aatgacaccg taaaaaattg
    34861 ccctttttca tcaaaggtaa atgtctttac aatgtgcaat ggattatttt gcacgatagc
    34921 acctgtaaaa aagcgtatcg aatctgtttc tttttgtaat ttccagtttg tataatcact
    34981 ttctgttgat agcaaaaatt ctccagttgt aaaatgtaca ggaaaatcaa aaatacccct
    35041 ggcaatgtat ttgcttttat tgaccacaag ctcaatattt cgggttttat caatgaacga
    35101 ggtacatgca gcgccatgat tggaaagcac ggctttataa taatgtgaat caacagtcac
    35161 ttcttccttt aagcgggata attccaggaa ttttgaaagt tgtttcaatt caggtttttt
    35221 aatttcaacc gtttgttcaa cttcaacttt cttatcttga actgcaggtt ttttttcctg
    35281 cggcggaaat aaataaaacc ataatatcca tatcagcaaa gataataaaa ctgcaattaa
    35341 agcccttttt tccataacaa cctcttgttt aactttctag atataaaata aaaaaatatg
    35401 actattattg aaatattata ataggtgaaa aggagtgata tgaggggtaa ctgtcaagaa
    35461 ttgtgctaag gaacaggatc atacccaccg tcacaatatg gatgacagcg tagtatccgc
    35521 ttgattgata aaaaaccacc tttacagagt ccatgtttgc tcaatgcttc tattgcatag
    35581 cttgaacatg ttggataaaa tctgcaaaca ttgggtaaca atggtgaaat aataagctta
    35641 tacattttta tcaataatat tggcaatgaa ataatattta tcttcattgt ttgagcactc
    35701 ctgatctaag caacaattgt tttaccaaac ctgaaaggtg agtatatggc attgccttac
    35761 aggttttatc aggaaaaata ataacagcaa aaccctgttg tatctgaggc aaaagagtat
    35821 taagtataga ttttattctt cttctgattt tgttacgttc aactgcatcc ccaaaatttt
    35881 tattaacagc tatggaaaaa tgtgaaactg actcattccc aataaacaat gtttcatgtg
    35941 ctggttgtat atgcactatc attcgtatac ctttactata aaatcgtctg ccattaacaa
    36001 taacatcttt aaaactcctc cgccctttta ttgaatatct ccttttcacc ggaaaataat
    36061 tatacaaata tatgctactt tacattacgt ttttcatctg acacagttag tttacaacgt
    36121 cccttctgtc ttcttctttt cagtatttct cttccagctt tggtactcat cctttctcta
    36181 aatccatgtg ttcgtaatcg cttgattaca cttggttgaa atgttctttt cattattcca
    36241 cctcattatt atgaaaatag tacgatacag tttcgcagtt aaatattcga taagtaagta
    36301 ttttattaat aattaatatg ttaaattgat aatatcaatt atagcttttt aataatagca
    36361 caaatttgta aataaacata acaaaattac aattttattt tttagaatgt ctatgtcaag
    36421 aatttattat attacttttt tgctattacc atgatagtat ctgaaaaaca aatactatca
    36481 gatagtttta tatatatttt tttatgtttt gatgccaaag agcaaaaccg ttcaggatga
    36541 tatccagaag gatagcactt tacttttaca aaacccatgt tttttaataa tcgtgtaata
    36601 ccacgtggtg atacatcaat tctatggtct ggggggtgat ttttaatcca gatatctctg
    36661 tgacaataaa acaatggtcc aaaatatgat ggtaacgtgc atgcaaaaat accatcatta
    36721 tgtaacatcg aatatacttt ttgaaacatt gcaaaaggat tagcaaaatg ctccataaca
    36781 taccacgccg ttataacatc atatttttta gctgtcagga acttttcaaa atcaactgta
    36841 gtaactgata tggaaaactt ttccttacaa tattgtgctg catattctga tatttctatt
    36901 ccttcaacga attgaaaacc actgtcttta gctgccttac aaaaaaaacc taatgcgcat
    36961 cctatatcaa gaagactctt ttcggacaga ttattattca gtaatttttt tattttttct
    37021 attctcagta atgaagcagt gtatattgcg tcatgatcat cttcgtatgt ttttccgtac
    37081 tgattcttgt agtcttcacc aaaataatca gcagtataag gtgatggttg atatgcattg
    37141 ttaaacaata ttttacatgg tttgcataaa aaaatatcgt ttgcaatttg ttttgatcgt
    37201 gagtccttgc agattggaca tagtaaaggt atcatttatc tcattgtttt ttcttttttg
    37261 ctggttctac tacgatagga atacctttaa tccttgcttt atcaagtgat actaatacaa
    37321 gttcagcata ttgttctggt atctcaataa acgaataagt ggaatgtata tcaattttgc
    37381 caaccagttt tccatcaata cccgaacgtt ttgatatctc acggataaga tcaccagggg
    37441 tgattttatg attcttacca atatttataa atatacgtgc ttttccattt ttaatatctt
    37501 caaagacaat tgttccgtca gattttgcca tgtttaatga cgttatttca gataaatcca
    37561 gttttgagat atccccgatg cactctttca aaagcgctgc agtaatcctt tttgttgaac
    37621 caaataaggg taaatgtttt ctcatagctt taatgagcaa aaaatatgaa gttaaatcct
    37681 cattcaaagc agattctaaa atcttttcaa tctttttaac ctgattcata attactcctg
    37741 aaaaaactga ttacaaaaac ttaataaagt ttttttacat taacgaaaaa aacatgtaga
    37801 ttggcaagca tatttctttc atcaatcctt acacttatag ccaacatttc tcactgtttc
    37861 aataatatta ttgaattcat cgcctatttt tgaccgcaaa cggcggatgt gaacatcaac
    37921 agtgcgtgta ccgccaatat actctatacc ccagatacgt gataataatt cctgacgtgt
    37981 aaaaactact cctttatttt caataaaaag ttttaacaat tcaaactctt taaatgttaa
    38041 tgaaactttt tcattgttaa ccgatacaat gtatttgtca ggttcaatag ctatatttcc
    38101 aactctaatg actgcatctt ttatttcaat ttcagagcct attaaacagt ctatcctgac
    38161 cttaagctca ccttttctga aagggaagat gataaaatca tcaaaaaacc aatccctgtt
    38221 tatagactca aggtatcctg tacgaacgat gagaataacc ggtacatgag aaaaacgttt
    38281 tcgtattttt gataaataaa attgtgcatc acttatgtcg atctgctcac tatcaaaaag
    38341 tattatatca aatccttcat ctttaagagt attaattgaa ccgtgaatgc ttgcatccag
    38401 gggttgtacc agtcgtactt ctgaaaccag ttctttactc cgggtaataa gtgctgtttt
    38461 aattttcatt catatccact aattgataaa aaatatccat atattgcttg gctacatttt
    38521 cggcataata agctgtaatt ctggctttat tattttttga acattgagca tagagctttt
    38581 tatcattttt aaatcgcatt atacattctg cacatttttg tatgtcgcct acatcaatca
    38641 gcaaggcatt gtgttcattt tctaaaaaat caacctgccc accggaatta gtggtaacaa
    38701 ttggcagtcc acaatacatc gcttccataa aaacaatccc aaacccctcg tgcaatgatg
    38761 ttaaaacaaa aaggtcggat actgataagt atgcatattt tttatcatca gatacaaacc
    38821 ctaaaaatgt aacaatgttt tcaagtgcca attcttggac cagttgttca agatactcac
    38881 gttcgggtcc atcacccatg ataatcaatt ttacatgttg attattaatc cgggctattg
    38941 ctttaatcaa cacatcaatt gcttttcgtt taatgagcct tccaattgtt atacagataa
    39001 aatctttttc atgcagccct aacaatttcc ttgagctctt tggtataaca ggaggatgaa
    39061 aagcaagagg tattatgcct atgtcctttt tgattttata gtagtggaag gcgttgtttc
    39121 gagtatttga ggattgtgct atgatagcat cagccttgtt aagaagatat tggactatta
    39181 atttgaagaa acgattgcga tgtggtgaaa attttttact ggggtcataa atatcaccac
    39241 catgaagcga taagacattt gggattttaa atagctttga tagcacatat cccaatggtc
    39301 ctgatggaac aacaaaatgt gtattgataa tgtcaaattt gtatttctta aagagcgaca
    39361 gtgctctgat aaaacctgct ggtaaatagc taaacatcga gataaatgtt gctgcatcac
    39421 gagactttct aaataaaatc ttaactctat ggacatgtat cccattgata tattcatatg
    39481 ggggtaaacc tttgaaagag gaagttatga tatgtacatt ggcaactttt acccactcta
    39541 aggctaaatc ataggttgca acacccccgc caccacctaa cggtgggaat tcataattta
    39601 taattaagat attgattttt ttgtgtaatt ccatgtggat tatcctatgg tattgtaaac
    39661 ttttttcatt tattgctgga attgaataat acttcattgc acttacgcta ttattgtatt
    39721 ttcttcaata atttttttac caacattgca atatttttaa agaactattt gttataacta
    39781 tgcgatgaaa taaaataatt gattgacaca ttgtacagtt tgtttatggt caataagaaa
    39841 ataacagaat gaattaatat aataaaaaag tatagcaaag gaatatggta taacaatata
    39901 cacacaaggt ggaatacaac atatagtgtt caatagagtg cttctatgag atagttgtcg
    39961 atggactacc cgtaggggat gaaaattgca tagtatcgtc atgctgaggc gaaaccgata
    40021 gtcattctga acgaagtgaa gaatctcgtc attttataaa cgaatagatt cttcggggct
    40081 gcgttcctca gaataacaat gtggggtgtg tcatcctgaa taatgttctg aaccggtttc
    40141 agtatcattt caggctctaa gattacatat ttaaattccg tggcaagccc ggaatgacga
    40201 atgtggaaaa agagattccg gacaaagtct gggatgacat tggcacatac ttctattttc
    40261 ggaggagcca tatgctcttt tgggtgggga agaaacattg agctgttgtt atacaaatat
    40321 gataaacgat agggtaatgt tatttcgagg ataaaaacta tcatggcaat catgttgata
    40381 gagaaaagat atttttgcca aaatgtaata ggttgtgcaa tgactatgaa tagataaatg
    40441 caatgtgaag atgcatcact ccgatacaaa cacactgagg gatggcatag accacgatat
    40501 tgtaattttt ttggaggagg gttgttgtga gtataaaaga tatactgatt tatttttatt
    40561 tacccgttat agtatttact gtattatgtt tttgtgcaat tgttgtaggt attatttact
    40621 ggataaaaaa atataaagaa cttttaataa ttctttttta ttttttaatc catgcaggaa
    40681 tgactgcaat gtatatttat ccttttaatg tatttgcgtt tagtttgcaa tatgcgttct
    40741 ttttattttg tctgttgatg ttttgtttta gtgcagtttt catggtgtta ccagatgcat
    40801 caaataattc agattcatcc tatagagcag tgcaatgtat tgttgggaaa agtattcttg
    40861 tttatgtcag tatagtgttt ttgacgccaa aagagcaatt aataataagc actgttgatt
    40921 gtatggtatc cttagtattt atatcagtga aacctaaacc tgcaacaagc tgtgtgcaac
    40981 catttgcaga aaaggaaaaa tttattttta taatcgtgat tacaattgtt tttttgtgga
    41041 taagtatagc aaaaatattt ttatttcatg ttttaatatt gggtatgact atccttgtca
    41101 ttgtcaatgt tgttttagta tgtgtcatag cattgaaggt atatgtctat aaagacatgg
    41161 attttattgc aattcagcag gaaaattatt gtggtatgta tgaaaaacat tttaaaattg
    41221 ttcagataat taaagaacat attaatgaat actacaccca cccaattgat aaatatacaa
    41281 tagcactcca atgtgagatg aatcctgatt atctttgcaa gatttttaaa agcaatgaag
    41341 gaattaccat caatgaatat ataaaaaata tacggttaaa gaaagcattg gaaacattgt
    41401 caaatactga taggaaaata attgaaattg ctttagatgt aggttatgcc aatttagcaa
    41461 ctttttacag gcattttaca cagttttata aaatatcacc aaatcagata aggataaaaa
    41521 aaagtaataa tataacaact gtttaagact aacaggtaca ttccacaata actatcgcac
    41581 aaataaacat gcatcattgt aaaggctgtt caaagattat tcagggcaag ctaaaaagtt
    41641 ttttaaatat ttttttgttg acttgaaaat gtaaatagta ctattattta ctgtttatta
    41701 agtattaatt taataattca tactacaaaa gtaaaaggtg gacagtatga ttgaattggc
    41761 agataaaggg aatggtacat ttgttgtaaa gattaatatg caagaggttc atacccttga
    41821 tgttcccgac cttaaagaaa aattgcaaca agctattgtg aataaaggga taaaacggtt
    41881 ggttgttgat cttgcagatg ttaaaatgat aacgagttcg gggataggta tatttttaaa
    41941 tattaatcag aatttgaaat cacaactacg cttagcagca ccaacaccgg aagtacaaaa
    42001 ggttctggaa cttactaaag taacatcaat gataaaagtt tttaattccg tagatgctgc
    42061 cctgcaaagt ttttaatgca aaactatcaa caaaaaaatt atttaccctg cttcatttat
    42121 gtgctatact tttatttaat tatcaattgt agtttttata gtattaatcg ttgtaataca
    42181 ttgtatatag cacgattata aagaatagta atcgtactat cttatcaaga gtggtagagg
    42241 gactggccct gtgaaaccac ggcaaccgga acgaaaagcc ggtgccaatt ccagcggaag
    42301 atattccggg agatgagata gcaataagta tccgcttaca cgatcttccg gtaagcgttt
    42361 ttttatttta agggggagtt gattatttac ttagatgaaa gcggagataa tacctggaaa
    42421 ttcggctggt atcgttacta ctaattattt cacttttgct gagccacctg atgaattgtt
    42481 attggtttca gggaaaaaac ttgggccaat aacattagcc tatgaaacat acggtactct
    42541 taacagtgac aaaagtaatg ccatattaat acttcatgcc ctttcaggtt cagcacatgc
    42601 ggcaggatac cattcaaaaa aagatatata tccgggttgg tgggatattt atattggtcc
    42661 cggtaaaact tttgatacat cacactattt tgttatttgt tccaatgtta ttggcggatg
    42721 cagcggttca accggtccat catccattaa tcctgaaacg ggaaaagaat atggtttgga
    42781 ttttcccatt gtcactattc aggatatggt aaatgcgcaa tatcatttaa ttaaattttt
    42841 ggggatacaa aagcttttat cagttgttgg aggttcaatg ggtgggatgc aggcattgca
    42901 atggtcaata tcatatcctg agatggtgca gtctgtagta gcaattgcaa cggcagcgtc
    42961 tctttcggca cagggaatag cattcaatga agttggcaga caggcaatta tgcttgatcc
    43021 caactggaat tatggtaaat attataacaa tgaaaatcct aagaatggac tatcacttgc
    43081 gcgtatgatt ggacatataa cctacctgag cgagaagctg atgcacgaaa aatttggcag
    43141 aagattgcaa cattcagata tttttaaatt tcacctggat cttgagtttg aagttgagtc
    43201 ataccttcac cataaaggga atgattttgt tcagagattt gatgctaact catatctgta
    43261 tatcacaaaa gctattgatt attttgattt gaagaatgat tttaatggtg atctggctaa
    43321 tgcttttgtt aatgtaaatt ctaattacct tgttataagt ttttcttctg actggttata
    43381 cccaacttca caatcgcgcg agattgttag agcgttgcgt atgaacagca aaaatgttat
    43441 ttttaccgaa atacagagtg ataaagggca tgatacattt ctactgccac atgaacaatt
    43501 ggagcataat atagctaatt ttttaaaacg ggagtttgaa aaggttcacc ggtaatgaag
    43561 gaatacaggg ttgcatatga tttgattcta aaagagattc catatggtgc acgggtgctt
    43621 gatttaggat gtggtgatgg tcagttgttg aagttgctac aaactacaag aaaagttaaa
    43681 ggatttggtg ttgaaatctc accggaaggt gtaagccaat gtgttgaaaa agggttgtac
    43741 tgttatcagg gtgacattga cgaagggctg aatgattata aagacaattc atttgattat
    43801 gtgattgtta atcagacgtt gcaaagtaca aaaaaaccag agattgttat acgcgaatcg
    43861 cttcgtatat cattaaatgc tatcttcagt tttcctaact ttgcccatta tagcctgagg
    43921 ttgtatattt caatctatgg ccgaatgccg aaaaataaat tattacccta tgaatggtat
    43981 gaaacaccga atatacatct tttaacaatt aaggacttca gagatttttg caatgcgttt
    44041 ggttatccta taaaaaaaga aatgcatttt tcaaccattc gttctcattc agtaacaata
    44101 cctattatgc ccaacctttt tgccgagtat ggtttttttg tattggatgg aggaccattt
    44161 acctctgata ctatatgatt ccatcccgtt gggataaggt attaagtttt ttctggtgag
    44221 tagtgcaaaa aaaaataaaa caaaaatttt ggctacaccc aacccaatgt ttttcttttt
    44281 taaaagataa taatatatcc ttcaaaggta ttccgctaca tcataatcgt tttactgata
    44341 gagtaattac tcttacaatt accagttagt gtagccaaaa taaactataa tgtattgatg
    44401 cagtttttta ttatctaata gtaaactttt acgataagaa attactattc attttatgtc
    44461 aaatattttt ttatattaaa ataaaaaaat atattattta tataatatgt tgttataata
    44521 tataacaaat aattatattt aatccttcta tttattgaaa ctattaaaaa cattgtgttg
    44581 aaattgttgc aatggctttc attgtatcaa aaagcatggt aaacccaata gtgataaatt
    44641 caggttaatt tgttgataaa attattgatt tcaaataacg tataataata gttgtatatg
    44701 ttcaacggat gcattggatg catttatagt tttataaatt atgtaataaa gaattgcagg
    44761 cgatagttac ttgaagattt cattacacct tattgatcca tttcaatgaa gattcaattt
    44821 gtacaacttc cgctgattga tcatggatta cagtacatta ccggtaatat ccccaatgct
    44881 gctgcaaccc tgcacgcctt ctgtgagcta acctctggtg gtaccatgaa aggattacaa
    44941 ctgccgtata gcatccagaa ctatggctct gattcgatga ttgtgaatca tattttgcac
    45001 aatgagccaa cgcatgttgc attcacttgc tatttgtgga atgtagagcg ttcactggca
    45061 attgctaaaa cgattaaggc tatggcatca catatcataa tactctttgg tggtccagaa
    45121 atacaggtaa actcatatat cctgaaacag caccaaccgt gcatagatat atttgtcatt
    45181 ggcacaggcg aatttttttt caatcattac tataataata cctgccagca gtttattcat
    45241 atggtcaatg gtaacgccgt ttttattcag ggctctgaca cctttattcc gctgtcacag
    45301 atagtggaac cctatacatc tggatacctc acaccaatgc atgataaaag cctgagtgtt
    45361 gaaattattc gggggtgtcc tttcaaatgc acctattgtc tctattctaa aaataccagc
    45421 agggtcagag ccttaccgcc gcacaagctt gccgatatta tcaaaaaaag tatgagtgat
    45481 gaaatacagg aactgtatct tttagcccct acatttaatc aatcatcaca cttcaatgat
    45541 tatctgcaaa cactgatatc cagcagatgc tctgtctcac tgcatactga gctcagggcc
    45601 gataccatta ccagcaacat tgctatgacc ttaaagaaag ccaatttccg tagcgctgag
    45661 gtaggcattc aaacattaaa taaaaaatgt ttacaaaaga ttggcagaaa aacaaacccg
    45721 gaaaaagagc tacaggggat attctattta aaagatgcag gcatcaattt acaggttggc
    45781 ataatccccg ggcttcccta tgatacccct gaatcatttt atacaacaat ggacactctg
    45841 cttgatgcag ggcttgggaa tgaactggaa ctttacccac tgatggtgct gccgggtacc
    45901 gctctgtatg atgaggcaat tgctgcaggt gcaacatttt tgcaaaagcc accttattac
    45961 ctcattgaag ggttcaattt tacgcagcat gatataacag ccatcacatg ttattttgaa
    46021 gacaccacag ggtttacctt tgatacccca tacctgccat cgttcatttt tcagcagggg
    46081 gagcttgtat cgggagcctg cattgacctt acaaagcgcc gtaccttccc tggggaagta
    46141 tttgattata tgcaaacaag tcatgttacc ttttatttgc tatgcgatag ttacccgcaa
    46201 attgaacatg ccgtagaatt aataatccag cagtatccat gggacacaat gctctgctca
    46261 attgtcctta tgtatgatgc agttcttaac gatgaggata ttgccagcaa agtatttaca
    46321 gcaacacata atacctttta ttgcaggatg atgtattttc aggataccta tgataagctg
    46381 cctgtgcgta tcttccaggt atttgaatca ttgaaaacat atctcagagc taaagaccat
    46441 taccgctgta tcaaccctat cattaaactg ttgcgcagca attatgatga aattacacag
    46501 tatcactttg cctatcctgt gccggctgtt gtggcaaaag gttgtgcagg gatatatggc
    46561 caatggataa aagaacagta cgctgaatgt attgatattg taagctttga ggatgatgat
    46621 gatcaaaata tgatatataa aactctgggg attgacggta aaccgacaat cccctttaca
    46681 acactctcta tttaaagcat ctcttttaac ggcaatacaa aaatgctaac gccagcatct
    46741 ttaaatttaa cctttaaaga tgctatatcg gacgcaatag catctttcat tttattatct
    46801 tctattgcta ccatcaaaca gttgttaagc cctccccata tatgcgtccc taattgtggc
    46861 tctttattcc cttcacccag tacacctaca aactttgtat acccttttgc atactttttt
    46921 gttatctcaa caacctcttc atcaacagca ttgtgatata caataaataa catcaccatg
    46981 gtaattcccc cttaaaactt tgctttatgt tttgccgaat acttttcacg cctggtttca
    47041 acgatcatgt atataacagg aataaatata agtgtaaaaa taaatcccgt actcatgcca
    47101 cccataacgg ctctgcctat tggttgtgac atttcagatc catcaccgcc actgaatgcc
    47161 atagggatta gcgaaagtac agttgtgagg gtagtcatca taattggcct gagtcgtttt
    47221 gttccggctt caaccagcgc ttcgcgtaaa ccccatcccc gttgtattaa aatatcagta
    47281 taatccacaa gaactatcgc attattgacc actataccta ccagcataat gatgcctaaa
    47341 agtgtagcaa cactgaactg tgaacctgtt aa
//
LOCUS       DAOOJH010000012        44287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_952_length_44287_cov_9.126515, whole genome shotgun sequence.
ACCESSION   DAOOJH010000012 DAOOJH010000000
VERSION     DAOOJH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 44287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44287
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_952_length_44287_cov_9.126515"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..1527
                     /locus_tag="PLJ75_03160"
     CDS             406..1527
                     /locus_tag="PLJ75_03160"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPD04246.1"
                     /translation="MSTTIHALTIQRYANNGFGIGFLHNKAVFIPYCAIGDVLTVEII
                     NSTKTYSFARIVDIVNPSPIRIQPRCPAFACCGGCDFLHIPYEEELSLKKNLFINTLQ
                     RIGGIPIQSVPGIEIIASQRFAYRSHATFQSNGADIGFFKKDSHVIQPLPQTGCLLVH
                     NDINRAIVTGKKNSGAIRIAVDHYNKIGSDPNDVITEIEQGIVYKRNPQLFFQANRFL
                     RGKMLSIVNELTDNATHFLDVGCGVGFFSLYMSKRMEGTGIDSNTLSITFAKMNSTIN
                     TIPCNFYATTIENYHPYTNCADCVIIDPPRQGISRKGRKTIIAMDPATIVYISCNPVT
                     FARDVRSFIDSNYRLQKLFMIDMFPCTHHTEVIGLMKKS"
     gene            complement(1570..2139)
                     /locus_tag="PLJ75_03165"
     CDS             complement(1570..2139)
                     /locus_tag="PLJ75_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPD04247.1"
                     /translation="MVSGQQNILKVTLSISICMHSAVLFFTFPAIQSTTPKEELTVTI
                     HGQTVLPYVEKAGDTTRLKKSTTPNSPAECHEQSITAAQNYTISGQDSEEVMMQYTDM
                     IRSRIQQALIYPSTARKDGIEGKAYVKFVIDSAGNVLSVAMVKSSGCALLDDESVKAI
                     YRASPFPRIPENLNKKSLSFVQGLTFTLK"
     gene            complement(2142..2537)
                     /locus_tag="PLJ75_03170"
     CDS             complement(2142..2537)
                     /locus_tag="PLJ75_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPD04248.1"
                     /translation="MDIERRKKVSVELNIAPLIDVVFQLLLFFALTTSYVTNPGIEVA
                     LPKAASAVPVQKEHIELYITKDNAVYCNNEKVTMGSLRKRLENMPSLSKTVILKADKS
                     VELGMVVQVIDVVKQTNIKDLVIATTQEE"
     gene            complement(2540..3130)
                     /locus_tag="PLJ75_03175"
     CDS             complement(2540..3130)
                     /locus_tag="PLJ75_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPD04249.1"
                     /translation="MIDIFLKGGPVMWPILFCSILSLAIFFERLYMLMKSRRYVWGAI
                     ARYKVEPLTDEGDSASCNGIVKDLLSYADNYSSNTLHDIEHRMYRFATVFIQGLEKRL
                     NWLAIIGNVAPLLGLLGTVTGMIRVFMAIQNMQGQVNPSALAGGIWEALITTAFGLIV
                     AIPTLVAYHYFEDRIDEMVNAIKDIIHVILENKHEC"
     gene            3702..3941
                     /locus_tag="PLJ75_03180"
     CDS             3702..3941
                     /locus_tag="PLJ75_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD04250.1"
                     /translation="MAGIKEKHVIQISSRRGRKKSPYLPPDYKTLQEVIDAIERDYIL
                     HTLELTNWNLQKTSRVLDIARNTLKAKMKKYNIDM"
     gene            complement(3948..4961)
                     /locus_tag="PLJ75_03185"
     CDS             complement(3948..4961)
                     /locus_tag="PLJ75_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04251.1"
                     /translation="MPDDKQYDSLSDQTQLINRALQYTNGNLEKAQQMVAGIFQDVAV
                     IKGKFKASKVGAFYVFLNIESNYIININAIVTTYTDIYNKIRIFDRWKQFYAMFGDIA
                     AELGASGGESSKFANHLADAIEGYNIYEPIKTGNMQLVSELFEEIIGKYFSMNADCQI
                     EIDRTTSLELENEDIPMELMDRQKADEAILTPDEMKMREIESQVEYIIPGSVIVSPVK
                     GKYINDIKQGEKISVMLTGKDPVSDRIARMFNAITSDGEYLPVNARIKEKFPLSSGGY
                     AIYALVAKNVMVKIIEEENVKIDIEKVEQKKEESNENMLFIYVALLLGLLFISALIVF
                     ALI"
     gene            complement(4962..5432)
                     /locus_tag="PLJ75_03190"
     CDS             complement(4962..5432)
                     /locus_tag="PLJ75_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04252.1"
                     /translation="MKQIIKKKDSGEDDSLFGLLASYKNLYSYITVIESPEQDIIAII
                     IAKNPSSFDELVKSKYQQFKPMRRIKIGVTRLFIKYITPEFIIKYFGPLLGIKVPENK
                     VHIINNLESGYFVLEIPGNYKEQVIQCIKRIVNLTPQKKYEIIEKSLADEEVII"
     gene            complement(5556..6110)
                     /locus_tag="PLJ75_03195"
     CDS             complement(5556..6110)
                     /locus_tag="PLJ75_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HPD04253.1"
                     /translation="MIVEIKKLAQGISVTRKSTDLFTRYVGRLLFQKIIEKLQHIAPH
                     EVVIIDFDGIRAVDASFVDECLVPLVKLSQTNETPFYGKLINISDNVEYIVGQVISME
                     KEHERYILMTDRLCKNGCHALGRLSEIEKDIIEYFVINRQATSDDIASFLHLSRDEAN
                     TVCSNLHKARAIRKIDDSYVFYCV"
     gene            complement(6115..7026)
                     /locus_tag="PLJ75_03200"
     CDS             complement(6115..7026)
                     /locus_tag="PLJ75_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04254.1"
                     /translation="MLFLKYKGLIVSTVSLPDQFKDPFSFETILSLHQTILSTKTAKL
                     DMTNITFIEPYSLLCLLLMGRNYLRLTGERLMLHRVPPHIMQYATRMDFFKYNIFTVD
                     ESLHGNAGLKRSASSSRLLEIIEIPGKEKESVKVIAKVIATFRKRAIVILKNWFTLTT
                     IDHLVTVISEICQNIFEHSLDSGFVVIQSYTYMNERIFRIAIVDSGIGVPGSFEHLPM
                     VLSLSNAKLLEAVLTTPLSSKRRYGYGLCQVNAIMEQLKGSIFLRSENAYISVLHHKR
                     AAKGSYIFLKDDCPTFPGTQFSITLAG"
     gene            7443..8216
                     /locus_tag="PLJ75_03205"
     CDS             7443..8216
                     /locus_tag="PLJ75_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04255.1"
                     /translation="MLTDHEIETLITKLRNKYNDYAHKYNARWFSVDAFEERLQTALR
                     NKINMEAFIVAEIAHFEKIKQRYEQKNTTSFSTKVDALIEELTARINHYPEIAFHPEA
                     HFEIMHMYGACNQLLELYFPVLWLIIDDRSTVYNFEQQLHYLCGRTSEKESKRIEDHI
                     ALLNRHGVKSIEIEKNKNEYLKECAFLLHAIHEWLNNILNNYDTTPLNFSKLYIRGDK
                     KHKIISLFNNETCRSALATIQNYIHQIIEDFRLQAFKKI"
     gene            complement(8213..9802)
                     /locus_tag="PLJ75_03210"
     CDS             complement(8213..9802)
                     /locus_tag="PLJ75_03210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04256.1"
                     /translation="MEPIAEQIKILVLDDDPFIGRMLTSMLIGYNVRCATTFNEFHTI
                     IEEFIPDIAIIDLFLPDANGLDVCKWIRNQSTYEDTILYILTSNTDEQTLVRAYSIGV
                     MDYIIKPFNKFILFSKLHRCALMIEIKRKLETSLEYQKDLKKRLLQLNDFVKKCITID
                     DLDSLVALMVYVQHIVPVDGLCVFAVNNNKYTPLELIPHQRSPKIITALYRAAAITKN
                     DSVLFFFDNKNNIYVSRIALQQHGFVFLVKEKQFNPDEKNMLTLFAELLKVIISRTEI
                     HDYVQKQNRIYQEEISKVRQIQVAQLPKFKNIHGFEIASTFLPAEDISGDFYDGFFIN
                     GNTDIFQIVLCDVSGHGVASSYIGNAIRALIRTYSKENIYPAQVLYKLNQQMAIDGKG
                     LFYYGTIVIVRLYPDGKIVYASGGHPPLYYFNSANQSIEEIQNTGPLVGIFYNIEFKD
                     SILNMNSKDMLLLYTDGIIESMDPELRKLYGFERLKNNIMAFSGEPVMDMLHSICGSM
                     YEFTGYAPLEDDVTLICIKKT"
     gene            10192..10674
                     /locus_tag="PLJ75_03215"
     CDS             10192..10674
                     /locus_tag="PLJ75_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPD04257.1"
                     /translation="MLLCYILIALCLLIVLKRVVKVVPADSICIIEKKHHYYKTLRPG
                     WHCITPFYSQARIVPVEKRNSIPVTKEITFGDSSRHAVTVSTTFLLWDPYTPFFTFEK
                     LDTSMSNQALKELETIASTCSMQEFTHTIDAIKQKCLDALNQFGKEYGIEFTQIIISL
                     "
     gene            10671..12515
                     /locus_tag="PLJ75_03220"
     CDS             10671..12515
                     /locus_tag="PLJ75_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04258.1"
                     /translation="MIVAKNLSKKFGNSLLFEDVTFSIGSKEKVGLVGRNGLGKTTLL
                     KIIAGTEQPDEGAITIPRHYTLGFMSQNLSFTKMVARDEALSLLPQSEQHEYWRAEKI
                     LSGLGFSNDDMVKRVSELSGGFQVRLQLAKTLILNPDMLILDEPTNYLDITSIRWLAR
                     FLQSWRGELLLVTHDRSFMDAIITHTLGIHRNHIKKIKGTTEQWYEQIAQEEEIYEKT
                     RINDERKQKEIQEFISRFRAKARLANLVQSRIKTIEKMQKREKLIPVKNLDFEFRYKP
                     NNAKYIMQVDSLTFGYNEKPLINNFSLSIASGDRICIIGPNGKGKTTLCELLAGTMKP
                     QSGIITRASGVEIGYFEQTNSIMLNPNATVEDEIVLSDPDKNRQLARNLCGLLLFEGD
                     DALKPISVLSGGEKSRVLIGKILVKPINLLILDEPTNHLDMEACDALLAAIDSFDGAV
                     ILVTHNELLLKALAQRCIIFQKNTITIFEGDYQRFLETIGWENESEPVKKEYENINEQ
                     KLNKKELRRIRSQLLMEKQNECKPLQETVSSIEQEITQHENLLKILNEEIINASQLQK
                     SDDIQQLSIEISKIQKQLSALYDKLEEIMSQHEERTRHYERLMQEYSL"
     gene            complement(12532..12924)
                     /locus_tag="PLJ75_03225"
     CDS             complement(12532..12924)
                     /locus_tag="PLJ75_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04259.1"
                     /translation="MKDIIAKILIVDDEDVVLKSCLRILQKLDYEVDTAYSAQTALEY
                     LQKKKYDIVVTDLMMPGMDGLQLLNEIKKLYPDTVVVIFTGFATVETTRQALKAGAFD
                     YIPKPFTPDELRTVIENAVKYCKNSNQK"
     gene            complement(12934..14886)
                     /locus_tag="PLJ75_03230"
     CDS             complement(12934..14886)
                     /locus_tag="PLJ75_03230"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPD04260.1"
                     /translation="MNKRFASQIRFKLFISILSIVLLSGVLSTFIGIRTIQKHVVGQA
                     FDTVLKDLDTADFIYTSRVALKSKMLKHISLLEYIQIAILHNDRAVLRSKCKEIQQEL
                     GIDIVNITDAQGKIIVRANNDSYGDDVSDDNQVSYVLKNKIPCEGTDVVLYKHLEREG
                     ESIASKAIIKIIPTPKSRKVQRTIIKDALTISVAIPIFHNNNFIGIIYGIRILNNYNG
                     IVDQIKQLVFKEEKLGGLDVGTSTIFLDDVRVCTNVKKPDGTRAIGTLLSEEVYNKVI
                     LQGKLWWDKAFVVNNWYISAYKPLLNIYGKPVGILYVGILEKKFDEIKKSSTLNFAFT
                     TALIAIIAVGISLYLLNIIIRPVRTLVKASESVINGNYDTHIEIETEDEIGYLCYTFN
                     QMIDAIKERDKKLKEQTQDQILQSEKLASLGRLASGIAHEINNPLTAILTYSSLLLED
                     LKETDFEEDLRVIIKETLRCRDIVRNILDFARSTKPDIKRTNVNNLINEVLLILEKHV
                     NFHNIAINKYLDPDVPDMFIDENQFKSVINNLAMNAADAMPQGGTLTVQTKYNFGNDS
                     ISIIVSDTGVGIKEEHLDKIFDPFFTTKEQGKGTGLGLAVTYGIIKRYHGNINVHSEV
                     GKGTVFTITFPLLKNKEEYSEYDKSK"
     gene            complement(15171..15572)
                     /locus_tag="PLJ75_03235"
     CDS             complement(15171..15572)
                     /locus_tag="PLJ75_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04261.1"
                     /translation="MAKKILLVDDDRDLVESLKQALVMNGYEVEVAYSGAEGLKKLLS
                     YKPDCLILDVMMETDTAGFEISYQIRSNRETSKYKDVKNVPIIMLTAINQVTNSRFSL
                     NEDQSFLPGINDFLTKPVRIDELLDKIKKYA"
     gene            complement(15595..16125)
                     /locus_tag="PLJ75_03240"
     CDS             complement(15595..16125)
                     /locus_tag="PLJ75_03240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HPD04262.1"
                     /translation="MFAKLSKKAFPKSLWVYHCNSGACNGCDIEILNILTPYYDVERF
                     GIKLVASPRHADVMLISGAVTRPTFPLVKKAYDAMPSPKIVFGIGSCATGGGCWFDTY
                     NVTGGADKAVPVNYFIPGCPPRPEAIIYGVALALGLVDKKAAPVELKQIEFPIDIYQR
                     TKAWEERNVIYQLLYD"
     gene            complement(16128..16673)
                     /locus_tag="PLJ75_03245"
     CDS             complement(16128..16673)
                     /locus_tag="PLJ75_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD04263.1"
                     /translation="MWLTSKIKQLFVVTKPGTVTLAYPFDPRPVPENFRGQPYWDHHK
                     CIGCGGCANHCPARTILVRDLCNEIRVMVYDGSRCTYCGRCADLCPEKAITMTPEYEL
                     ATNDKRDVNETMELFMLTCQRCGRCFDHEIKNAIDRLTYLGYRYDNLEVRAVIPVSTQ
                     VFDKDMYEKTMTYKRPGKIGE"
     gene            complement(16698..17597)
                     /locus_tag="PLJ75_03250"
     CDS             complement(16698..17597)
                     /locus_tag="PLJ75_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPD04264.1"
                     /translation="MDILLILLNIILFLGLAPLYEGIIRKLTAKIQSRQGPPIYQPYL
                     DIMKLLGKDNLVSGNWAFKLAPMMAFAAVTAVITIVPIGFKPNYLTHYADIITIVYLL
                     TLAGISILLGALSSKNAYAMIGASREMVTMIMVEPILAMTFILGAVKMKSLAIDASMF
                     SVLSNGYSWSVVLMLVVYVMALQAFVARQPFDISEAEVEILEGPFIEYSGPNYALFKY
                     SMMLKQMFYAVLFVTVFMPFLFTGIFIIDVLLQLIAVAVVFAVIALLGSTNPRLRIDQ
                     AVKYYAVLIGISLCTIAMSVYGI"
     gene            complement(17610..19283)
                     /locus_tag="PLJ75_03255"
     CDS             complement(17610..19283)
                     /locus_tag="PLJ75_03255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPD04265.1"
                     /translation="MPSIDQIKSELTAKFKGSLKGFDTPLPTRLFVDVDKNSIRSLCN
                     AVVGMGARYLVSIGYDNIARDNTLGLVHTFSFDNDNVFCCVRTKVPADNPVCDSITPD
                     IPNAGWSEREYMDLLGMKFEGHPKPKRLILADDWPAGIYPLRKEVPWNLMPPAAEDVA
                     YQLDEAPEGCTTVPIGPFHPTLHEPAHFAVYVDGETIKGCDYRGFMTHRGIEKLCTTQ
                     VTYNEVPFIAERICGICGSVHATSYSQAVELAAGITISRKAEYIRTFMLELERIHSHL
                     LWLGVAGHLIGFDTVFMHSWRVREPVMWLAEKITGNRKTYGMIIIGGVRRDILQHKDD
                     ILNVMNTVEKELIAIKNAIVGDTVIHRRTKGVGYVSTEDTVKWSLVGPVARARGVNID
                     TRRDHPYAAYDAVKFDVPVVDTCDVWGTLLVRILETFESIKIVRQVLNDMPVDEPLLY
                     EFDEPIKPLIHSIAAVEAPRGEVVHYLITGEENRADRWRVRAPTYPNLQGVPLMLLNN
                     QLADAPIIIGSIDPCFSCTDRMALVDLTTGKHEVLNGEQFEKLSRSKKG"
     gene            complement(19309..21171)
                     /locus_tag="PLJ75_03260"
     CDS             complement(19309..21171)
                     /locus_tag="PLJ75_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPD04266.1"
                     /translation="MNIDVYGILLQAMAVLLGAGLVVPLLANKRKLAGWINTIVIAIA
                     SSLLLYVAYNAIFVMESSETQAIYFGGVCVYFLIDAFSGFFIAIIAFMAVISALYSID
                     YMEHYKDYSLRPYFINFPLFILGMVCIVTVDDLSIGFTVAWQLMTIASYFLIRFEYKN
                     KENVRNANKYLILMELAWVFIVTGAFIINGSAAGDSIHTLTQKIGASTGIVPVIVYTL
                     LFLGFSFKAGVFPFGQLWLPDAHSIAPSPISALLSGVMIKTGIYGIIRTFFWMVPHDG
                     NTINPLVWGAIIATFGVVTLFIGTSQSLKQSDAKRLLAYSSIGQIGYIVLAIGAGLYM
                     YHSSNMFVKMLSFVAIIGILYHVLNHAIFKGLLFLTSGSILYETGTKDLNKLGGLMTF
                     MPISAVIAGIASLSIAGVPPFSGFASKWTIISATLLGGSQVFFLVLFGIMALFTSALT
                     LACYVKFFGMSFASTGVEWNGDHKPHEVSWKMLLPKCVLAILCILQGIIPFVYYDTFI
                     KIFKSSSGSIIQHAFAELSTQNYIFTNAYGVTVNVPGMHYANAVAVPLVMIVIIIIGF
                     AVAYMVRKSAGAQEKVMPTWLCGYQDLNNNNRYKDKHMFAALKSILWWTGGNVK"
     gene            complement(21161..21664)
                     /locus_tag="PLJ75_03265"
     CDS             complement(21161..21664)
                     /locus_tag="PLJ75_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPD04267.1"
                     /translation="MSFTALQNIFNTTTCIVGLGNYYRHDDAVGLFILDRLKAIDSNP
                     GVLLFNVEDIIESYVFTIAESNCNNVLIIDAIVGEAEPGTIVFGKLHDIVETGLNIST
                     HKLALQMSCKIWEASQKDVYLLGIVINNDDYGKGLSPVIHDKAETIVNMLQASIMSNQ
                     KECVYEH"
     gene            complement(21661..22494)
                     /locus_tag="PLJ75_03270"
     CDS             complement(21661..22494)
                     /locus_tag="PLJ75_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPD04268.1"
                     /translation="MSEQKIVTVPEIATIEEIKDEIVDVKTFYLRFDNKEIDGNFKIK
                     SGQFIMCTVFGAGEFAVSLPPSPENDRFHITVRKIGKVTNALHDLEVGDKVGVRGPFG
                     NGFPFEEIKGKNVIYVAGGIGLIPLRSSIVHVLQHRNEFGRILLLYGSRSPQDLMYQY
                     MLEQWQKIEGFETFITVDNGTPEWKGNVGIITTLFNKVEIPVENTVAFVCGPPVMFNA
                     VIKELMQRGIKDDMIISTLERHMKCGVGKCQHCAIGRTLVCTDGPVYTYRQIKILGEQ
                     I"
     gene            complement(22504..23556)
                     /locus_tag="PLJ75_03275"
     CDS             complement(22504..23556)
                     /locus_tag="PLJ75_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPD04269.1"
                     /translation="MYLIKRDDIKKIAHALEEKYTLYGPYLDEVYGQCIFDTVNGDTV
                     NVNAPIPTNPPKQVVFPHLEKIMQYKYDKKAKKAEIAMLFDDAPKALFGLRSCDLTGL
                     QCIDRFFLGQEFVDEVYRHHRSRMFIVSSTCVVPFKQCFCVCTDSGPAANEGYDINLT
                     DLGDSYLAEVGSEKGKAFIEAFHFPAATDEHAKLKQKLVEKSITMFEDIAVESKAWVS
                     RVMNRVTTGFIKEEVWEYIGKQCIECGACAYVCPTCTCFNINDTSTVEGDTYRLRTWD
                     SCSYEGYSRMAGGHNPRKPIEDRRNKRFFCKLSYSQSKKYLRPGCVGCGRCAWVCPGD
                     IGLPNVVTYIRREIHE"
     gene            complement(23558..25525)
                     /locus_tag="PLJ75_03280"
     CDS             complement(23558..25525)
                     /locus_tag="PLJ75_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPD04270.1"
                     /translation="MSYLPGVCTFCGTGCGHYLKVDGASVNGVFPSRVHPVSKGRLCV
                     RGWNIHELLSTHDRITKPHVKEGEVSYRDAITKLVSALSSYKPEEIAFYVSPRSSNED
                     NFALMKLARAVFKTNHISIQSESGHRNSLDVLFKGTGCAGMLGSLEDIRKAEVIIVAG
                     MDITTQNPIVGSELHYAARNGSLLVTIDSRTTKIARLSKKFMNIKPGTMRQVFDAIAY
                     YIDSEKWGKTTIAKYDEYRRYLETVPFKTIEEVAGVSVSDIMFVAEKLAKAKSAAVFF
                     TSGISGLQYDTVASIYNCFAIADKIGREGCGVNPVTGINNLQGSFDMGCAPDLLTGFQ
                     SIDDTAAKKRFEQQWNTKLPELKGRSVEELLADSTSPLKALVIVDHDDGIIKFADRLK
                     SLPFVAYIGAFTNKVADFAHVVLPITTYIETDGTFTNAERRVQLSVKKCNPAFDVLPL
                     WKLASDIAAAAGKPFNYAHASDIMDEIASLTPCYEALSHKALAKKHGIQWPCTKKNPD
                     GTKYCATDKPAFVLPQKPYAMVQANESYPYLLMLGKAQHYWHQNTIMHKTFIPLREYN
                     ATLLLYPDGYVAIAPEDAKKLGVRDRWPVKVVAEKASMKAMVMISDDVAPGTAYAPYF
                     IRDSITSFLLAYSDVVSQGEEATIPVRIEKV"
     gene            complement(26146..26835)
                     /locus_tag="PLJ75_03285"
     CDS             complement(26146..26835)
                     /locus_tag="PLJ75_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPD04271.1"
                     /translation="MKYEILGKPDFPCVKINLNAGESIVAESGAMVAMSTNMEIKTES
                     RGGIMKSLKRSVLGGESFFINTFTASDKAGEILLTSATMGDLEVIELSGNDIILSTHA
                     FVCSSQSVSIDSTWGGFRAFFGGEGLFFLKASGSGTLFFSSFGAIHQIQLQGEYIVDT
                     GHIVAFDPSVSFTIEKVGGLKSLFLSGEGLVCRFSGSGRLWIQTRNQNSFAAWADQFR
                     RVESSDNSDTM"
     gene            complement(26845..27519)
                     /locus_tag="PLJ75_03290"
     CDS             complement(26845..27519)
                     /locus_tag="PLJ75_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPD04272.1"
                     /translation="MNINIKYNPSYALAVVSLNADEQIKAEDGAMVSKSVNVSIETKK
                     ASSSGIMKSAKIAFFGGTSFWLNTFTAQGSRGEVTLAPTLPGDIKKLDIAGTVFVQSS
                     SFLAASPSLEINTKFQGLKGFFSGESLFFLQISGDGPLLIASYGGIEELTVSESLIVD
                     TGHIVAFQDSLTYRIEKLGNWKSFFLGGEGLVCHFTGSGKIWVQSRNVSSLTSWLREK
                     LPPKRE"
     gene            complement(27523..28185)
                     /locus_tag="PLJ75_03295"
     CDS             complement(27523..28185)
                     /locus_tag="PLJ75_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006883275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HPD04273.1"
                     /translation="MEYVFKHNPTYTLLALKLAPDEKVKAEAGAMVYMSPEISIETGF
                     GSGFLSAVARKFFGGESLFVNIFTASHGGGEIGIAPQYVGDIQHKKMLGEAIMLQSGS
                     YLCSSPELTVTPQFGGLRSLFGGEGLFLLRVSGNGDLFFSSYGSIIEIPVSGNYVVDT
                     GHIVAFEDSLSFAVKKVGGWKSTFFSGEGLVCEFAGSGKLWIQSRVPNSFINWITKLL
                     PR"
     gene            complement(28320..31169)
                     /locus_tag="PLJ75_03300"
     CDS             complement(28320..31169)
                     /locus_tag="PLJ75_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04274.1"
                     /translation="MSTILVIDDKKDNLVSITALLKNLIADCMVITAEDGKEGIEKAK
                     QSNPDVIILDIKMPGMDGFDVCRILKDDPMTRNIPVIFLTAIHTDTHSKVKGLKLGAD
                     AFLTKPIDQAELIAQINAMMRIKSAEDMLRKEKDLLEDMVLQRTGELLASQMRYKSLF
                     NSISDMVIIHKSDGNILEVNTTTCNRLGFSHESLAMMTLQQLTSEGDSNRIHTFLQSI
                     SENTDWYETAFVDSHGNTIPVEIASTTIYYRDENAVLSVARDITERKQIEQVRTQLSI
                     LLDNSDDAIIGTTLDGTIASWNKAAEKIFGYAFDEAAGCHVSLLSPPYRPDEFEQLIE
                     RVKHNEGIAHYESEGVDRHGAIHNMSMTVTPLEDERGTIIGCSIIARDLTDVKRTKEK
                     LKQGLDIIQNLEDIAPAYFLTLSEEGTVITCNKAMLQATGYKASEVTGKSCCTLFIPD
                     FGQRAFRNQLSHMKKTKATHIFETSIVMKDATERIVQWHARPVYDADVFNYFMLIGID
                     MTEKKQLEKKILQANIDERNRIARDLHDGLGQHLAGIIFKTEMLRLKLKDEFSNEVPE
                     IVEIQSMVHQAIEHTRLIAQGLSPVDLNNKGLHVSLQEIIADIDKNFKMKAILEWDVT
                     SNIDALVDTVHVYYIVREAVNNAVKHGNPKHIAVSVCEDDNDYCIRIMDDGRGIPDDV
                     DVTKGMGMHIMQYRAWIINAALQIQNNKTGGTLVQCTIHKSLKPVTEKLKSTKKFSIV
                     VVDDHPIVRQGLQQLIASHKNLEVIGEARSADEAIDVINKRVPDCVLVDISLNGTSGI
                     ELVKALKQRFPKLPALVLSMYDEKLYAERAIKAGARGYLMKNEAPDKIINAIYTVLKG
                     DIYLSGEVKELIVNALSDRGVIDSSVEGLTNREFEIFQLIGHGLGNKHIAEKLHISVK
                     TVENVREKIKMKLHLDSSQDLLQYAIQWVREHGGD"
     gene            complement(31166..32221)
                     /locus_tag="PLJ75_03305"
     CDS             complement(31166..32221)
                     /locus_tag="PLJ75_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04275.1"
                     /translation="MNKEFLHSISHELRSPLNSILTLSKVLLMQADGRLTPEEKEYLA
                     IIERNGSRLLALVNDLIEVFEIDSGIELHITDVMVSTILSDILERYEGILEKKGLLLH
                     THLDDVILQSDETRVYQVFEHLIDNAVKFTEEGCITVSAHAHDGMCTVSIADTGIGIR
                     SEFLDTLFTGFTKASGSLSSKYPGTGLGLTIVLKLLSILGGTIHVESQLGKGSCFTVQ
                     LPLQVEAKPQADRKKILVIDDDSDSVVVVKALLSKLYDITETQSGTRGIALAHEVKPD
                     CILLDLSLPDLHGLEVVKELKSDDHTKHIPVIIVTASSLKNERAVIMASGCDDFIQKP
                     FPIEEFVLKVQHWVSQS"
     gene            complement(32214..32711)
                     /locus_tag="PLJ75_03310"
     CDS             complement(32214..32711)
                     /locus_tag="PLJ75_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04276.1"
                     /translation="MTKPAELFIKSKNLDEFRGDIIACDGNFDFDIEDMIALGNAYFE
                     RFPDCFSNRNCQDVQLGYQLVRICLVEKLLSGFPATVKDAFRKIFFSASAVADTMKNL
                     QATSEYQELSNIVATINTRLEEYNRIIDLLPKGMIKERFVGGITNLFNIAYLIKMNFQ
                     QKAHE"
     gene            complement(32869..33840)
                     /locus_tag="PLJ75_03315"
     CDS             complement(32869..33840)
                     /locus_tag="PLJ75_03315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04277.1"
                     /translation="MKMQVTAIIMVCCAAISCARDITKVTIAKKPLPDNFTVVMPEKV
                     AVSNDAVTCTIYAKKFAQGNVAYCEIIPAKDVQLVSVKFIDKEIVLSKKTWGYRGFFA
                     IDPELKPGVYHCIIYGDSFHIEIPVTIKRSFFPVSVIPMEFDKYSQKDYIETPEVKQF
                     IAECTKKKQKAFATRFDDCIQSIPYHPRNTHTINSAFWAKRRYLLYDSSTKKRQTVTR
                     IHRGIDLNGNSGDPVYAMLDGIVVLADKLFYEGNMVIVNHGNGIFSYYMHMEKLMVSS
                     GDRVVAGQLIGAVGSTGMSTGPHLHVSLMVHGVQANPLSLLVLPIRE"
     gene            34039..34392
                     /locus_tag="PLJ75_03320"
     CDS             34039..34392
                     /locus_tag="PLJ75_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04278.1"
                     /translation="MSIQISFSKLEKEFMPQFRERITTSEDIVDVQKIFSYTIKEMLQ
                     KAFEKEGITINEENIALTSSAPYYTITINNHSINELWNTSDLKDILQRFAEVAHKRYI
                     HLKKNMAKTKMKIRR"
     gene            complement(34402..34773)
                     /locus_tag="PLJ75_03325"
     CDS             complement(34402..34773)
                     /locus_tag="PLJ75_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HPD04279.1"
                     /translation="MYIFELIAEQRIRDAMERGEFDNLPGMGKPLPRDELDTIPPDIR
                     MAYKIMKNAGIIPEEVELRKDIYSLQQLINACCDDDKRLEGLVKQLNAKQLRYTILME
                     QKGIHVPDYEYRKKVMEKLAK"
     gene            complement(34760..35071)
                     /locus_tag="PLJ75_03330"
     CDS             complement(34760..35071)
                     /locus_tag="PLJ75_03330"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04280.1"
                     /translation="MVLKEHVISQLQHNFLSKGVDRVILFGPFARNTQTGKSDVDLIV
                     IKQTDKRFFDRYDDFNDIESYVCVPVDCLIYTPAEWEIIYNRIFFKNVLKKATEIHVH
                     L"
     gene            35190..37001
                     /locus_tag="PLJ75_03335"
     CDS             35190..37001
                     /locus_tag="PLJ75_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04281.1"
                     /translation="MKPLLYIVPILMLSHLAFANEYWQRQIESLQKEQKQVHKQPYAS
                     FVLEYIDARLAMAQELSALYHESLNNKHSEHAATTHYVTVLSQCFDDAAINITRQQVI
                     QQLQPLQTSDTIDCTTNDYYIIHTELVLASITSYTSTGIEEKDNDTMNNDMSAPGAPP
                     MANLSASVRFNELYQKACHTKQKAEYIQSILTLCSGDTTSTAQYMHNPSLLLQLFPSL
                     AAKIHTKTIYSNVYNSGNGIPYQITIPHEIDSQKAVNDINAKRDELIATSTVPTSEID
                     AIAHAYIDPVHDAIAEQQKLLATMQSINGITIENEEALNTFIEHYTTQSRYLLDYAQA
                     NTLYCNLVQFKEPAIDYQKRYEHIATLAHQLQQQTIAGDSSGKTMPAIERIVSILKAF
                     ACTQHKKENDAAVQNLMQSLHTIKEAIRSRCTAPENNNLNPALCSREIAAGIETIEKG
                     IHLFQSQHYAKEALINYATVVNEAFEQASTGYGSNILQQIVTLQSAIPAVSNFDIQKI
                     ITEYTGQCYLLRKLRYDCASLVQRLQFFEKQGIAMPQKDKAFQLLETMKTLQPLYSVD
                     VYKYTMNQNNIIIIDRQCAAALTRISQKNKMLTAKDI"
     gene            37214..38284
                     /gene="mnmA"
                     /locus_tag="PLJ75_03340"
     CDS             37214..38284
                     /gene="mnmA"
                     /locus_tag="PLJ75_03340"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPD04282.1"
                     /translation="MNIAVAMSGGVDSSVAAALLVKEGHNVIGLTAKILLCSEMDGSQ
                     PRFDVCCSPENLHDAKKVANSLGIHHYTVNLEDEFYCNVIEPFCNEYCSGRTPSPCIN
                     CNTSIKFPKLLHHAKELGCDALATGHYASVCKNSRYYIKRGKDADKDQSYFLFNLNQE
                     ILAHVLLPLGLYTKEAIRAIAHDMGLHVAHKPESQEVCFVPDNDYPRYIEQHIGKRLP
                     AGDIVTTDGKVIGRHNGIHRYTIGQRRGLGIAWQHPLYVVAIDAANNRIIVGSNDELL
                     SSGLIASGINFMKATALNNTPVWIKTRSTQKPCKATATTDNGRLIITFDQPQSSITPG
                     QAVVCYDDDGAILAGGWIKEKL"
     gene            38330..38611
                     /locus_tag="PLJ75_03345"
     CDS             38330..38611
                     /locus_tag="PLJ75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HPD04283.1"
                     /translation="MIIRANDIKTKGVLLFEKLIAPGEDIIINVRGKNKYVVLDIERY
                     KQLRELELDTAYEKVMDDIKAGNYKKQTAREHVDDIINELYNNNNRDIP"
     gene            38574..38846
                     /locus_tag="PLJ75_03350"
     CDS             38574..38846
                     /locus_tag="PLJ75_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012857774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HPD04284.1"
                     /translation="MNYTIIITETYRKKVIKFFRKHREMIHTYAKVLSILETNPYHPS
                     LRLHKLQGKLHEYYSISLDTQYRMLIVFNIDESTIIPIDIGTHDEI"
     gene            complement(38913..40736)
                     /locus_tag="PLJ75_03355"
     CDS             complement(38913..40736)
                     /locus_tag="PLJ75_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPD04285.1"
                     /translation="MKKVFYSIRLSQMPLVMMDIACVIAGYVMASMLLHGMGYAVMKV
                     AAVHAVCTIAGLYVAQCYRIVWRYATIRDFYRVGEGFVLGGVLLLIAAYGITIPDAAT
                     LFIACGTGFVLLIAYRIVLRELTSSSVTIAPGKKSVILIAGAGEAGQTIATEFKRNGR
                     YDSIIGFVDDDKKKVGTLLSGKPVLGTTGDIPALVQQYTVKECIIAMPSAPKKAVDRI
                     ISIIQSCDHPVTISILPAVSRLFEKALTPELKGIGVEELIGRQEIQVDSNAIEQFFAG
                     KVVLVTGAGGSIGSEACRQLLHFRAKTIVAVGRGEHSIYNLTLMLNDYAKYFDKPDIR
                     YYIADINDATRMRYILDNEKPDVVFHAAAHKYVDIMEHNPQEAVRNNCLGTVTVLEAA
                     KHAGVKQFVCISTDKAVHPVSVMGATKRIAEMVTLAYNNSMRTCCVRFGNVIGSRGSV
                     IPLFYKQIAKGGPVTITHPDMTRYFMSIPEAVMLVINATVLSQGGEIFVLDMGKQYRV
                     EEVARNLIALLGYQPDVDIPIVYTGVRPGEKLQEELWNEGEELVPTAHTKIYTIAHNG
                     VDKELVNEVVSLKADHVQSLSAVECKSLIQHFVKDYNPYKC"
     gene            complement(40740..>40958)
                     /locus_tag="PLJ75_03360"
     CDS             complement(40740..>40958)
                     /locus_tag="PLJ75_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPD04286.1"
                     /translation="LAERGINTSVHFIPLYRFTYFKKRLGDSYRTFPSAEWVFEREIS
                     LPIYPSMKDEEIAYVIDNVIDIAQHYKR"
     assembly_gap    40959..41058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<41059..41770)
                     /locus_tag="PLJ75_03365"
     CDS             complement(<41059..41770)
                     /locus_tag="PLJ75_03365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPD04287.1"
                     /translation="MNIPFHVPYIDDDDIDAAVGAIKSGWTTMGPKTIAFEDAFARYV
                     NVPNAVAVSSCTAALHLALKVCGVGKDDEVIVPANTFVATAEVVEYCGATVVMADIDR
                     ETHCISVKSVKQLLSPKTKAIIPVHYAGHPCDMDAIMDSAKAHNCYVIEDAAHSLPSY
                     YKGKLAGTVGHCGCYSFYATKTLSTGEGGMLVTANNRWADQARSLRLHGMNRDAWKRY
                     DNHGSWYYDVEQQGYKYNM"
     gene            complement(41867..>44287)
                     /locus_tag="PLJ75_03370"
     CDS             complement(41867..>44287)
                     /locus_tag="PLJ75_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04288.1"
                     /translation="YQQFTSQYNVIVPLLSYYRVYRSDELSQTWYGNPLFWYSSHSTP
                     HDSEYTWWAPIIPLTYHHVRNNYGHRNIVWLLDYSWDEHGLQRLWCLPVFMWGKDLSK
                     ATIEHYTVTPLFGRRTVYDTSTQHYTYNNIWFPVIPVYYHSAGDDSSHTNILWLIDWR
                     RNHNALHTLWIVPLLFHEFTDNGYRYYVPLYCSPRRTAYEGVSFGLFHFHRWQPDMDR
                     LWIWLYYHSKHYAINNAPQKNGVEQYYIHFLPIYFSWRNDTSKGNILLPFVYNYSDTN
                     TTVHINLTGYARKTYTGIFKPDVGVNLSQSEGRKYLDTDVSWLYAMWSFSSRIPYSRD
                     KANTGLGDSYLQNADAVDNNKPADYDADSALMKRTIANNHLNGSDTTHPQINKKKTVN
                     REDSQFFWGWKIFFGLMAFEKADSMRHFRLLPLSWLTWDEASKDKLFVLLPLYVSYYS
                     DAEEYFVLFPFYGYQRDNNAFASMYLINCYWHEYDTAGYDEKTILWPFINWYHSDTKR
                     GFRIFPFYWFSVYTEDGYKVTRSFSLIHYNKEYSNNGLIAKSLYINPLYYKSVNTMDK
                     HDDTFAIPLCLMWQHRDDDGQSLFVAGYYRYRSKSYTRDTMLFLYDYKNYYSSGEAIK
                     TQHSLLLTTMTVTTEPEVLRLRLLWGALFEYDRDKNKPMYDVDALLWLVGVEEDNYSF
                     SHKILPLYYYYSEHQFNLLIIPVLLSGCYRHYYKIVDLGLLGLLYYRNEDLLMNDGRR
                     MVLLGTIFDETKKPERKYHSVGSLWGILWAYEKEEETGFTKFSILKGLYKYTEKDGSG
                     KHTFLWFL"
BASE COUNT        13571 a         9735 c         7676 g        13205 t
ORIGIN      
        1 ttctgttcaa tatgtcgaca aaacaggctt taaagtactc ctcctgcatc atatcacgcc
       61 actcgataca gcggctcatg atgtgatagg tatgctcggg cagttgtagg cgcaatttcc
      121 gtgccatggg tattcctccg tgtttttatt ataatgtaca tccctactat acataacgcc
      181 ggagatagaa aatattaaaa agaattggtg tatttgttga tactttttgc gatttttttt
      241 gaaaaaacac tatctacaag gggtcagagc atatattcac aaattttaat tgaatattga
      301 tccctaactt ccaacagtat ccttatacta tgtaatcatc attggacaat tgcataggta
      361 tagcatgata tctttttacc atcaatagtt atccagtatc tatatatgag cacaacaatt
      421 cacgcactca ctattcagcg ttatgccaat aacggttttg gtattggttt tttacacaat
      481 aaagccgtct ttataccata ctgcgccatt ggcgatgtcc ttactgttga aattataaac
      541 agtacaaaaa cctattcatt tgccaggatt gttgatattg ttaacccttc gccaattcgt
      601 atccaacccc ggtgccctgc gtttgcctgc tgcggaggtt gtgatttttt acacattccc
      661 tatgaagagg aactatcgct taaaaaaaat ctttttataa acacactgca aagaattggt
      721 gggataccca tacagagcgt ccccggtata gaaatcattg cctcgcaacg ttttgcctac
      781 cgaagccacg caacatttca atcaaacgga gcggatattg gattttttaa aaaagactct
      841 catgtcatac agccattacc acaaacgggc tgcctgctcg tacacaatga cattaatagg
      901 gcgatagtta ctggcaaaaa aaattccggt gccatcagga ttgctgttga tcactataat
      961 aaaataggct ctgaccccaa tgatgtcatc accgagattg aacagggtat cgtttataag
     1021 cgaaatccac agcttttctt ccaggcaaat aggtttcttc gcgggaaaat gctttccatt
     1081 gtcaatgagc ttaccgacaa tgctacacac tttttagatg ttggatgcgg cgtgggattt
     1141 ttcagccttt atatgtcaaa acgaatggaa ggcaccggca tagattcaaa tacattgagc
     1201 attacatttg caaaaatgaa ttcaacaatc aacactattc catgcaattt ttatgcaaca
     1261 actatcgaga actaccatcc gtatacaaac tgtgcggatt gcgttattat cgaccctcca
     1321 cgtcagggta taagcaggaa gggacgtaaa accatcatag ctatggaccc ggcaacgata
     1381 gtctatattt catgcaatcc tgttaccttt gctcgcgacg tcagaagctt tattgatagc
     1441 aactatcgcc tgcaaaagct tttcatgatt gatatgtttc cgtgtaccca ccataccgag
     1501 gtcattggat tgatgaaaaa aagctaatca caggatatta ccatgcattg gcaaacaatt
     1561 acaaaaccat tatttaaggg taaacgttaa accctgaaca aacgatagtg attttttatt
     1621 gaggttttca ggaatacgtg gaaaaggtga tgccctgtat atagccttca ctgattcatc
     1681 atccaacaaa gcacatcccg atgatttcac catcgcaacc gacagtacat tccctgcact
     1741 atctattaca aatttcacat aggctttacc ttctatgccg tctttccgtg cagtcgaagg
     1801 atatataagt gcctgctgaa tacgacttcg tatcatgtcg gtatactgca tcataacttc
     1861 ttcactatcc tgacctgaaa ttgtgtagtt ttgtgctgct gtgattgatt gttcatgaca
     1921 ttctgcggga ctattgggcg tggtactttt ttttaaccgt gttgtatccc cggctttttc
     1981 cacatagggc aatacagtct gaccatggat ggttactgtt aactcttcct ttggggttgt
     2041 tgattgaatt gctggaaatg taaaaaaaag cacagcacta tgcatgcaga ttgaaatgct
     2101 taacgtcact tttaatatat tctgttgccc actcaccatt gtcattcctc ttgcgttgtt
     2161 gctatcacaa gatcttttat attagtttgc ttaaccacat caatgacctg caccaccatc
     2221 cccagctcta cactcttatc ggctttcagg attactgttt tagataacga gggcatattt
     2281 tccagccttt ttcttaaact acccatagtt accttttcat tattacaata tacagcatta
     2341 tcttttgtaa tatacagttc aatatgttct ttttgtacgg gaacggctga tgcagccttt
     2401 ggtaacgcta cttctatacc gggattggtt acatatgatg ttgtaagcgc aaaaaataaa
     2461 agaagctgaa agaccacatc aataagcggc gctatgttaa gctcaactga aacctttttt
     2521 ctccgttcaa tatccacagc taacactcgt gtttattttc cagtattaca tggattatat
     2581 cttttattgc atttaccatc tcatctattc gatcttcaaa ataatgatat gcaacaagtg
     2641 ttggtatggc aacaataagt ccaaatgctg tggtgataag tgcctcccat ataccgccag
     2701 caagcgccga aggatttacc tgcccctgca tattttgtat agccataaat acccgtatca
     2761 tccctgtcac tgttcccaat aatcccagca gtggtgcaac attgcctatg attgccagcc
     2821 agttgaggcg cttttctaaa ccctggataa aaactgttgc aaagcgatac atcctgtgtt
     2881 caatatcatg caatgtgttg ctgctgtaat tatctgcata tgacagtaga tcttttacaa
     2941 tcccattgca tgatgcactg tcaccttcat ctgttaacgg ttctacctta taccgggcaa
     3001 ttgcccccca cacatatctg cgtgatttca tgagcatata taaccgctca aaaaagattg
     3061 caagcgatag tattgaacaa aaaagtattg gccacattac cggcccgcct tttaaaaaaa
     3121 tatcaatcat aggttcacac cgttacaaat tatttataat tgctacaata tgaatatcat
     3181 actgaaaata atgcaacagt tttatgtttt tttcacagtt tcttccctac aattgctttt
     3241 tcctcaaatc agcttcgtgt aacaacagtt ttaccactat tttgttcttt ttatgattta
     3301 tccagtggta catattctgg ataaaacatg atacctgtac tataacatat tcacagtttg
     3361 aaaagattca aaaagtgtta aactttacat cagaatgagt ggcagttttt acagcaataa
     3421 gcaagcggta taacctgttt tttcatcagc agtaatactt cgcaatatat taacattatt
     3481 aatattatgt ctattattta ttataagact attccagaaa acaattatat caaaatataa
     3541 atattttttt aataatataa aaaatgtatt gaatattttt atgggaatga tttaagctga
     3601 accagtcata ctatagttta atatatgaca ttttttagat atcattttac atattttgta
     3661 tattagtaat ttaaattttt ttgatgagca aggagttctc tatggctggc ataaaagaaa
     3721 agcacgtgat acaaatcagc tcccgcaggg gcagaaaaaa atctccctat ctacctcccg
     3781 attacaaaac actgcaggag gttattgatg caatagaacg tgattatatt ctgcatactt
     3841 tagaattaac taattggaat cttcagaaga caagccgtgt attagatata gcccgaaata
     3901 ctttaaaggc taaaatgaag aaatacaata ttgatatgta ataattatca tattaaggca
     3961 aatactatga gggcagatat gaataataaa ccaagaagga gagctacgta aataaataac
     4021 atattttcat tgctttcttc ttttttttgt tctacctttt cgatatcaat ttttacgttt
     4081 tcttcttcaa ttattttaac cattacattt tttgctacaa gtgcatagat agcataaccg
     4141 ccactggaaa gaggaaattt ttcttttatg cgggcattga caggcagata ttcaccatca
     4201 cttgttatag cattaaacat gcgtgctatt ctatctgata cgggatcctt tcctgtaagc
     4261 attacagaaa ttttttcacc ctgcttaatg tcatttatat attttccctt aacaggtgat
     4321 acaatgaccg aacccggaat aatatattca acctgtgatt ctatctcgcg cattttcatt
     4381 tcatcagggg ttaatattgc ctcatcagct ttctgtctat ccattaactc catgggaata
     4441 tcttcatttt caagttcaag tgatgtcgtc ctgtctatct ctatttgaca gtcagcattc
     4501 attgaaaaat atttgccaat tatctcctca aaaagctctg acactaactg catattgcct
     4561 gttttaattg gttcatagat attatatcct tcaattgcat cagctaaatg attggcaaac
     4621 tttgatgact cgcctcctga agcacctaat tcggcagcaa tatcaccaaa catggcataa
     4681 aattgcttcc acctatcaaa gatacgtatt ttattatata tatcagtata ggtagtaact
     4741 atcgcattga tattaataat ataattgctt tcaatattga gaaacacata gaatgcacca
     4801 actttactgg ctttaaattt tccttttatt accgccacat cctgaaaaat ccctgcaacc
     4861 atctgctggg ccttttccag attcccattg gtatattgta aagctctatt aataagctgg
     4921 gtctgatcac tcaaagaatc atattgctta tcgtccggca tctatattat tacctcttca
     4981 tcagctaaac ttttttcaat tatttcatat tttttttgtg gtgtcaggtt aacaatgcgt
     5041 tttatacatt gtattacctg ttctttataa ttaccaggta tctccagtac aaaataacca
     5101 ctttcaagat tattaataat gtgtacttta ttttctggaa cttttatacc aagcaatggc
     5161 ccaaaatatt taataataaa ttcgggcgtt atgtatttaa taaataacct tgtaacacct
     5221 atttttattc ttctcattgg tttaaattgc tggtatttac tttttactaa ttcatcaaat
     5281 gacgatggat ttttagctat gataatagct atgatatcct gttctggcga ttcaattaca
     5341 gtgatataac tgtaaagatt tttatatgaa gccagcaatc caaacagcga atcatcttct
     5401 cctgaatctt tttttttaat tatctgtttc atagagtagc attctattat agtatcaaaa
     5461 aataaatgcc gttattgcaa gagctatcgc ctgcaatgga gcgtcacttt ttaactcaat
     5521 actatatata aaggcattat taatcaagta ttttactaaa cgcagtaaaa aacataactg
     5581 tcatctattt ttctgatagc tcttgcttta tgaagatttg aacacacagt gtttgcctca
     5641 tctctggaca aatgcaaaaa agaagcaata tcatcagatg tagcctgtct gttgataaca
     5701 aaatattcaa taatgtcttt ttcaatttca gaaagcctcc ccagagcatg gcatccgttt
     5761 ttacatagtc tatcggtcat aagaatatat cgttcatgtt ctttctccat actgattacc
     5821 tgacccacaa tgtattcaac attgtcactt atattgataa gtttaccata aaaaggagtt
     5881 tcatttgttt gcgacagttt tacaagaggg acaagacatt catcaacaaa ggaagcatca
     5941 actgctcgta tgccgtcaaa atctatgatg acaacctcgt ggggagcaat atgttgcaat
     6001 ttttctataa tcttttgaaa cagcaatctt ccaacatagc gtgtaaatag atccgtactt
     6061 ttacgggtta cgctaatgcc ttgcgctaat ttttttattt ctacaatcat gcatttatcc
     6121 agcaagtgtt attgaaaact gtgttcccgg gaatgttggg cagtcatcct ttaagaaaat
     6181 ataacttccc tttgccgcac gcttatggtg cagcacactg atatatgcat tttcagatcg
     6241 taaaaatatt gatcctttaa gctgttccat tatcgcatta acctggcata gtccataacc
     6301 atagcgtcgt ttacttgaca atggtgtggt caataccgct tcaagcaact ttgcattgga
     6361 caaactcaaa accatgggta agtgctcaaa cgatccggga acgccaatcc ctgaatcaac
     6421 aatagcaatc ctgaaaatac gttcattcat ataggtataa ctctggatga caacaaaccc
     6481 tgaatcaaga ctatgttcaa aaatattctg acatatttcg gatataacag taacaagatg
     6541 gtctatcgtt gtcagggtaa accagttttt taaaatgacg attgcccgtt ttcggaatgt
     6601 agctataacc tttgcaataa cctttacgct ttctttttcc ttcccgggga tttcaataat
     6661 ttccaataaa cggctggatg aagcacttcg ttttaatcct gcattcccat gcaacgattc
     6721 atctacagta aatatattat acttaaaaaa atccatcctt gtagcatact gcataatatg
     6781 aggcggaaca cggtgcagca taagcctttc accggtaagc cgtaaataat tacgccccat
     6841 gagcagcaaa cacagcaatg aatatggctc tatgaaggta atatttgtca tatcaagctt
     6901 tgcagtttta gtggatagta tagtttgatg caaagaaagt atcgtttcaa agctgaaagg
     6961 gtccttgaat tgatcgggta aggatacagt actcactatc aatcctttat attttagaaa
     7021 tagcattcag gatgattaca tcccctgcta tactcatcga catttttgta aaaattttca
     7081 aacggtattc ctctatttta ttgttacctg cgtagcatat taacagtaat gtactattcg
     7141 attttttcaa catattttta tagcatgggc gatagttact gttcatgggc aatactgcac
     7201 gataatagct ataccgctga taattatttt tttgttgcaa tcactttgcg acaagtttac
     7261 actgtaaatg agattatcca gctttgttga tgataaccaa tggcaacacg tttaagaagt
     7321 gaacatggat gcactatgat gaaatgttca atcctgtgca gatcgcatta tggatacaat
     7381 gcagtacaat acgatatcat gcagttatat aatgaacaat aactatcgaa cataaatttt
     7441 ttatgcttac agatcatgaa attgaaaccc tgattacaaa actgcgtaat aaatataatg
     7501 attacgcaca taaatacaat gcccgctggt tttccgtcga tgcctttgaa gagcgtttgc
     7561 aaaccgcatt gcgtaataaa atcaacatgg aggcttttat tgttgctgaa attgcacact
     7621 ttgaaaaaat aaaacaacgc tatgagcaaa aaaacactac aagcttcagc acaaaagttg
     7681 atgcgctcat tgaagagctc actgccagga tcaaccatta tccagagata gcattccatc
     7741 cagaagccca ttttgaaata atgcacatgt atggtgcgtg taaccaactg ctggagctgt
     7801 attttccagt attatggctt atcattgatg acaggtcaac ggtgtataat tttgaacaac
     7861 agcttcacta tctttgtgga cgtacatctg aaaaagaatc aaagcggata gaagatcata
     7921 ttgcactatt aaaccgtcat ggtgtaaaaa gcattgaaat agaaaaaaat aaaaatgaat
     7981 atttaaaaga gtgcgcattt ttgcttcatg ccatacatga gtggttaaac aatattctta
     8041 ataactatga cactactcct ctcaattttt caaaacttta tatacgtggc gacaaaaaac
     8101 acaaaataat aagtttgttt aacaacgaga catgccgctc ggcacttgca acaatacaaa
     8161 actatatcca tcaaataata gaagatttta gactgcaggc atttaaaaaa atttaggttt
     8221 tcttaataca gataagggta acatcatcct ccagcggtgc atagccggta aattcgtaca
     8281 tgctgccgca aatggaatgc agcatatcca taacaggttc accggaaaat gccataatat
     8341 tattttttaa acgctcaaaa ccatataatt ttctcaattc agggtccatg gattcaataa
     8401 ttccatcagt atataacaat agcatgtctt tactgttcat gttcagaata gagtctttaa
     8461 actctatgtt ataaaatatt cctactaaag ggcctgtatt ctgaatttcc tctatactct
     8521 ggtttgcact gttaaagtag tacagcggcg gatgaccccc tgatgcatac acaatcttcc
     8581 catcaggata taatctaaca ataacaatgg taccatagta gaagagccct ttcccatcta
     8641 ttgccatctg ttgatttagt ttatacagca cctgtgcagg gtatatattc tctttactat
     8701 aagttcgtat taatgcgcgt atggcattgc cgatatatga tgaagcaacc ccatgccccg
     8761 acacatcaca taacactatc tgaaaaatat cagtattgcc gttaataaaa aatccatcat
     8821 aaaaatctcc cgagatatct tctgcaggta aaaatgtaga cgcaatttca aatccatgaa
     8881 tatttttaaa ttttggcaac tgcgcaacct ggatctgtct tactttggat atctcctcct
     8941 gatatattct gttttgcttt tgcacataat catgaatttc agttctgctg ataattactt
     9001 ttagcaattc agcaaacaat gtgagcatat ttttttcatc aggattaaac tgcttctcct
     9061 ttactaaaaa aacaaatcca tgctgctgca gtgcaattcg tgaaacatag atgttgttct
     9121 tattatcaaa gaaaaacaaa acgctgtcat tcttagttat agcagcagcc ctatataatg
     9181 cagtaataat ttttggtgaa cgttgatgtg gtattagttc taatggtgta tatttgttat
     9241 tattaactgc aaatacacat agcccatcaa caggaacgat atgctggacg tacaccataa
     9301 gagcaaccag tgaatccaga tcatctatag ttatacattt tttaacaaaa tcattaagct
     9361 ggagcaaacg tttttttaag tccttctggt attccagtga ggtttccaat ttccgtttaa
     9421 tttctatcat cagagcgcac cggtgcaatt ttgaaaataa tataaattta ttgaatggtt
     9481 tgatgatata atccatgaca ccgatgctat aggcccgtac aagcgtttgc tcatcagtgt
     9541 ttgatgtcaa tatatagagt attgtatcct catacgtact ttgattccgt atccatttac
     9601 acacatcaag cccgttggca tctggcaaaa aaagatcaat aatggcaata tcaggtataa
     9661 attcctcaat tatagtatga aattcattaa aagttgtagc acacctgaca ttatacccta
     9721 taagcataga ggtcagcatc ctgccaataa acggatcatc atccagcaca agtatcttta
     9781 tttgttcagc aatgggttcc atatttttta cctgcaaata tatatctttt atacacaaac
     9841 atttccatat tttcaagtgt atttttattg gtatttttcc aaaaatagca atatgcaccc
     9901 ctgtcattcc gggcttgacc cggaatctct tttgccactg ccgtcattcc ggaatccata
     9961 tgagtaacgt tatatcctgc aatgaattca ggattacgtc ctgcgtttca ggatgactat
    10021 cgccaaaaaa tactaaaaat cttttattga aattattgca aaaattacta tcattgtata
    10081 ctattagcat tcagttttac tataaaattt tggttaacga tattgcgtga ccttaattgc
    10141 tattgcagct tcttaattat aattatttaa atattttatt ggaggtaggt tatgttattg
    10201 tgctacatac ttattgcgct ttgtttgctc atagtgttaa agcgagtggt aaaagttgta
    10261 cccgctgatt caatatgtat aatagaaaaa aaacatcact attacaaaac gttacggcct
    10321 ggctggcatt gcattactcc tttttattct caggcacgta ttgtacccgt tgaaaaacgc
    10381 aacagcattc cagtaactaa agaaataacg tttggcgata gttcccgaca tgcagtcact
    10441 gtatcaacaa catttttatt atgggatccg tacacccctt tcttcacctt tgaaaagctt
    10501 gacacaagca tgagcaacca ggctctcaaa gaattagaaa caattgcttc tacctgctca
    10561 atgcaggaat ttacccatac cattgatgct ataaaacaaa aatgccttga cgcattaaac
    10621 cagtttggaa aagagtacgg aatagaattc acacaaataa taatatcact atgatagtag
    10681 caaaaaacct ttcaaaaaaa tttgggaatt cattgctctt tgaggatgtg accttttcta
    10741 ttggcagtaa ggaaaaggtt ggacttgttg ggcgtaatgg gcttggcaaa acaacccttt
    10801 taaagattat tgcaggaaca gaacagcctg atgaaggcgc tataaccatt cccagacatt
    10861 ataccctggg cttcatgtcc cagaatctta gctttacaaa aatggtagca cgtgatgaag
    10921 cactgtcttt gcttccccaa tctgaacaac atgaatactg gcgagccgag aagatactat
    10981 cagggttggg tttttcaaat gacgatatgg taaaaagagt ttctgaatta tccggtggat
    11041 ttcaggtaag gctacaactg gcaaaaacat tgatactaaa ccctgacatg ctcatactgg
    11101 atgaaccaac caactattta gatataacat caatacgatg gttagcgcgt tttttgcagt
    11161 catggcgggg tgagctttta cttgttaccc atgacagaag ttttatggat gcaataatta
    11221 ctcatacact gggtatacat cgtaatcaca taaaaaaaat caagggcacc acagaacaat
    11281 ggtatgaaca aattgcgcag gaagaagaaa tttatgaaaa aaccagaata aacgatgaac
    11341 gtaagcaaaa agaaattcaa gagtttattt cacgtttcag agcaaaagcc cgtcttgcta
    11401 atcttgtaca atcaagaatt aaaactatag aaaaaatgca aaaaagagaa aagctcatcc
    11461 cggtaaaaaa tttagatttt gaattcaggt acaaacccaa taatgcaaaa tatataatgc
    11521 aggttgactc gttaaccttt gggtataacg aaaagccttt aatcaataat ttttctttgt
    11581 ccattgcttc aggtgataga atttgcatca ttggccctaa cggtaagggg aaaacaaccc
    11641 tttgcgaact tcttgctggc accatgaaac cacaatcagg gattattact cgtgcatcag
    11701 gagttgaaat tggctatttt gaacaaacca atagtattat gcttaatcct aatgctactg
    11761 ttgaagatga aattgtgcta agcgatccag ataaaaatag acaacttgcc cggaaccttt
    11821 gtggtttgct tctttttgag ggtgatgacg cattaaaacc aatctcagtt ctttcaggtg
    11881 gcgaaaaaag tcgtgttctt ataggaaaaa tactggttaa acctattaac ctgctcattc
    11941 tggatgaacc aacgaatcac cttgatatgg aagcatgtga tgcgctgtta gcggcaatag
    12001 atagttttga cggtgccgtt atactggtaa ctcataacga actactgctc aaagccctgg
    12061 cacaacgatg tatcatcttt caaaaaaaca ctatcaccat atttgaaggc gattatcaac
    12121 gttttttaga aactatcgga tgggaaaacg agagtgaacc tgttaagaaa gaatatgaaa
    12181 atatcaatga gcaaaaattg aataaaaaag aattgcggcg tatccgttca caactattga
    12241 tggaaaaaca aaatgaatgc aaaccattgc aggaaacagt atcatcgata gagcaggaaa
    12301 ttacacagca tgaaaatctt ttaaaaattc ttaacgaaga aattatcaat gcatcacagt
    12361 tacaaaaaag tgatgacatc cagcaacttt ccatagagat cagcaagatt caaaaacaac
    12421 tatctgcttt atatgataag cttgaagaaa ttatgagcca gcatgaggaa agaacacgcc
    12481 attacgaacg actgatgcaa gaatattcat tataataaac taaaaaacca gctacttttg
    12541 attgctgttt ttacaatact taactgcatt ttcaataact gtgcgcaact catcaggggt
    12601 aaatggcttt ggaatatagt caaatgcgcc agccttcaac gcctgcctgg ttgtttctac
    12661 tgttgcaaac cctgtaaaga tgacaacaac agtatcagga tagagctttt ttatttcgtt
    12721 caatagctgc aatccatcca taccaggcat cattaaatca gtaaccacaa tatcatactt
    12781 cttcttttgt aaatattcca gtgctgtttg agctgaataa gcagtgtcaa cctcataatc
    12841 taacttttgt aaaattctga ggcagctttt caacactaca tcttcatcat ctactatcaa
    12901 tatttttgct ataatgtctt tcatagttag tacctatttt gatttatcat attctgagta
    12961 ttcttcctta ttttttaaca atgggaaggt tatggtaaat actgtcccct ttcccacttc
    13021 gctgtgaaca ttgatattgc cgtggtaccg cttgataata ccataggtaa cagccaatcc
    13081 taaacctgtt cctttgccct gttccttcgt tgtaaagaag ggatcaaata tcttgtcaag
    13141 atgctcctct ttgataccaa caccagtatc agaaacgatg atagatatgc tgtcattccc
    13201 aaaattatat ttggtttgaa cggttaacgt accaccctgt ggcatagcat cagcagcatt
    13261 catagctaaa ttattgatca ctgatttaaa ttgattttca tcaataaaca tatccgggac
    13321 atcaggatca agatacttgt tgatagcaat attatggaaa ttaacatgtt tttcaagtat
    13381 taacaaaacc tcatttatga ggttattaac attggtacgt ttgatatcag gtttggtgga
    13441 acgggcaaaa tccagaatat tgcgcactat atctctacat cgcaaggttt ccttgatgat
    13501 tacgcgcaaa tcctcttcaa aatcagtctc tttaagatcc tccagaagca gtgaactgta
    13561 ggttaaaatt gctgtgagcg gattatttat ctcatgagct atccccgaag ccaaccgccc
    13621 cagcgatgcc agcttttcag attgtaaaat ctggtcctgt gtttgttctt tcaacttttt
    13681 gtcgcgctct tttatggcat caatcatctg attaaatgta taacacaggt atccaatttc
    13741 atcctctgtt tcaatttcaa tatgtgtatc ataattccca ttgatgaccg actcagaagc
    13801 tttaaccagt gtcctcactg gccgaataat tatatttaat agatacagtg aaatgccaac
    13861 agcaatgatt gcaatcagcg ctgttgtaaa tgcaaaattc agagtagaac ttttttttat
    13921 ctcatcaaat ttcttttcca gaatgcctac ataaagaata cctaccggtt tgccatatat
    13981 attcaaaaga ggtttgtatg cagagatata ccagttgttc acaacaaatg ctttatccca
    14041 ccacagctta ccctgcaata ttactttatt gtatacctcc tctgataata atgttccgat
    14101 tgcccttgtc ccatcaggtt tttttacatt ggtacaaacc cgtacatcat ccagaaatat
    14161 tgttgatgtg ccaacatcta aaccaccaag cttttcctct ttgaatacaa gctgtttaat
    14221 ctgatcaact atcccattat agttgttcag tattctgatg ccatatataa ttccaataaa
    14281 gttattgtta tgaaaaattg gtattgcaac actaattgtc agagcatctt ttatgattgt
    14341 tcgctgcacc tttcgtgatt ttggtgttgg tataattttt ataatagcct tgcttgcaat
    14401 ggattcgcct tcccgttcaa gatgcttata taatactaca tctgtacctt cacatgggat
    14461 tttattcttc aacacatacg aaacttgatt atcatcagat acatcatctc cataactatc
    14521 gttgtttgct cttactataa ttttaccctg tgcgtcggtg atattgacaa tatctattcc
    14581 caattcttgc tgaatttcct tacatttact cctcaaaact gctctgtcat tgtgcaggat
    14641 agctatctga atatattcca gcaaagaaat atgctttaac atcttgcttt taagagctac
    14701 tctgctggta taaataaaat ctgctgtatc cagatccttc aaaactgtgt caaatgcctg
    14761 cccaacaaca tgcttttgaa tagttcgtat acctataaac gtcgatagta cacccgagag
    14821 cagcactatt gacaatatac taatgaacag cttaaagcgt atctgtgatg caaatcgttt
    14881 attcattgca ggactattat ttattattta ctattattga ataccataaa ttatattgca
    14941 tttttatgca atcctttttc attgttacac aattactaac ggatattgga aaatgttaat
    15001 gttcataccc ccgcggccac taataaatat tggttccatt gactgttaac aatacccata
    15061 agaaacaaaa ctattcgcat acagaaaaat aaccggaaca tcaaatatga tattccggct
    15121 ttgcgattgt aaacaggttg atttgatact atatgcacat cactgtaaac tcatgcatat
    15181 ttcttaattt tatctaaaag ctcatcaatg cgaacaggtt ttgtaagaaa atcatttatc
    15241 ccgggtagga acgattgatc ttcatttaac gaaaatcgag aattggtaac ctgattgata
    15301 gcagtaagca tgatgatagg tacattttta acatctttat atttagaagt ttcccggtta
    15361 ctcctgatct gatatgatat ctcaaaacca gcagtgtcag tttccatcat gacgtccaga
    15421 atcaaacaat caggtttgta cgatagtaac ttttttaacc cttctgcacc actgtatgca
    15481 acctccactt cataaccatt catcaccaga gcctgcttca agctttcaac cagatccctg
    15541 tcatcatcaa ctaataatat tttcttagcc attgtgtgca ctccatttca aattttagtc
    15601 atagagtaat tgataaataa catttctttc ttcccatgct ttggtccgtt gatatatatc
    15661 gataggaaat tctatctgtt tcaactcaac aggtgcagcc tttttatcca caagacccag
    15721 tgccagcgca actccataaa taattgcttc tggccgggga ggacatccgg gaataaaata
    15781 atttactggt actgctttat cagcaccccc cgttacatta taggtatcga accagcatcc
    15841 accacctgta gcacatgagc caatcccaaa aacaatcttt ggagatggca ttgcatcata
    15901 agcttttttt acaagcggaa aggttggacg tgtaacagct cctgatataa gcattacatc
    15961 agcatgccgt ggggaagcaa ccagtttgat gccaaaacgt tcaacatcat aatatggcgt
    16021 cagtatattc aatatctcaa tatcacaccc attacatgca ccggaattgc agtgatacac
    16081 ccataatgat tttgggaatg ccttttttga taatttagca aacataacta ttctcctatc
    16141 ttcccgggtc ttttataggt catggttttt tcgtacatat ctttatcaaa tacttgagtt
    16201 gatacaggga taactgctct cacctcaagg ttatcatatc tatatcccag atatgtcagc
    16261 ctatcaattg cattttttat ttcatgatca aagcatctgc cacatcgctg gcaggtaagc
    16321 ataaaaagtt ccatcgtttc attaacatcc cgtttatcat tggttgctaa ttcatactcc
    16381 ggggtcatcg ttatagcttt ttcaggacag aggtctgcgc aacgtccgca gtaagtgcat
    16441 cgagaaccat catacaccat taccctgatc tcattacata agtcgcgcac caatattgta
    16501 cgcgctgggc aatgattggc gcatccacca catccaatac atttatggtg atcccaatat
    16561 ggctgacccc taaaattttc cggaacaggc cgtggatcaa atggataggc taatgttacc
    16621 gtccctggtt ttgtcaccac aaaaagctgt tttattttac ttgtaagcca cacgatcgta
    16681 ctccttatgt tttagaatta tattccatac acactcatcg ctatggtaca cagtgaaata
    16741 ccaatgagca ctgcgtaata tttcaccgcc tgatcaattc gcaaccgcgg gttggtagac
    16801 cctaacaatg ctatgactgc aaacacaact gcaactgcta taagctgcaa taatacatcg
    16861 atgataaata taccggtaaa caaaaacggc ataaacactg tcacaaacag tactgcatag
    16921 aacatctgct ttagcatcat ggaatattta aatagagcat agttgggacc actgtattca
    16981 ataaatggcc cttccagaat ctctacctca gcttcagaga tatcaaatgg ctgtcgtgca
    17041 acaaaagcct gtaatgccat tacatacact acaagcataa gcaccaccga ccacgaatac
    17101 ccatttgaaa gcacactgaa catacttgca tctatagcaa ggcttttcat cttaaccgca
    17161 cccaaaataa atgtcattgc taagattggt tctaccatta tcatggttac catctcacgg
    17221 cttgccccaa tcatcgcata ggcatttttg cttgaaagcg ctcccaataa tatagaaatc
    17281 cctgctaacg taagcaaata cacaatggtg attatatccg cataatgggt aagatagtta
    17341 ggtttaaatc ctatgggaac tatggttatt accgcagtaa cggctgcaaa tgccatcatt
    17401 ggcgccagct taaatgccca gttgccacta acaaggttat ccttccccaa tagcttcata
    17461 atatctaaat atggctgata gataggtggc ccctgccggg actgtatctt tgccgtcaac
    17521 tttcgtatta ttccttcata caatggtgcc agccctaaaa acaatataat gttaagcagt
    17581 attaatagaa tatccatata acacctacct tacccttttt tacttcgtga tagtttttca
    17641 aactgttcac cgttcaacac ctcatgtttc cctgttgtaa gatcaacaag tgccatcctg
    17701 tcagtacatg agaaacatgg atctatactg ccaatgataa tgggtgcatc ggctaattga
    17761 ttgttcagca acatgagcgg aacaccctgc aaattagggt acgtgggggc acgtacccgc
    17821 catctgtcgg ctctgttttc ttcaccggtg atgagatagt gtaccacctc accgcgtggt
    17881 gcttcaaccg cagctatgga atggattagt ggctttattg gctcatcaaa ttcatataat
    17941 aatggttcgt caacaggcat atcgttcagt acctgccgta ctatcttaat tgactcaaag
    18001 gtttccagaa tacgaacaag cagcgttccc catacatcac aggtatcaac cacgggcaca
    18061 tcaaacttca ccgcatcata cgctgcgtag gggtgatcac gcctggtatc gatattaact
    18121 ccacgtgcac gagcaacagg accaaccaga ctccatttga ctgtatcctc tgtcgaaacg
    18181 tatccaacgc cttttgtcct tcggtggatg accgtatcac caacaattgc attctttatt
    18241 gcaataagtt ccttctctac cgtattcatt acatttaaaa tatcatcttt atgctgaagt
    18301 atatcccttc gcacaccacc aatgatgatc atcccgtagg ttttacgatt tccggtaatc
    18361 ttttctgcaa gccacattac cggttcacga acgcgccacg aatgcataaa cacggtatcg
    18421 aatcctatta agtgtcctgc aacaccaagc cacaacaggt gtgaatgtat tctttcaagc
    18481 tccagcatga atgtgcgtat atattccgcc ttgcgcgata ttgttatgcc tgctgcaagc
    18541 tcaacagcct gagaatacga tgttgcatga actgaaccgc aaatcccgca tatacgctcg
    18601 gcaataaatg gcacctcatt ataggttacc tgtgttgtac aaagtttttc aataccacgg
    18661 tgtgtcataa aaccgcgata atcacatccc tttattgttt caccatcaac atatactgca
    18721 aaatgagcag gttcatgtaa ggttggatgg aatggcccaa tgggtaccgt tgtacatcct
    18781 tccggtgcct catctagctg gtaggcaaca tcttcagctg ctggaggcat caaattccat
    18841 ggcacctcct ttcgcagggg ataaatacca gctggccagt catcagcaag gataagccgt
    18901 ttgggttttg gatgtccctc aaatttcata ccaaggaggt ccatatattc tcgctctgac
    18961 caccctgcgt ttggaatatc aggggttata gaatcacata caggattatc agcaggcacc
    19021 tttgtgcgaa cacagcaaaa aacattatcg ttatcaaagc taaaggtatg taccaatccc
    19081 aatgtattat cacgtgctat attgtcatat ccaatgctta ccagatatcg agctcccata
    19141 ccaacaaccg cattacacag agagcgtata ctgtttttat ccacatcaac aaacaagcgt
    19201 gttggcagtg gcgtgtcaaa acccttcaat gagcccttaa actttgccgt taattcactt
    19261 tttatttgat ctatcgatgg catattctac ctcgtactta atgttgtgtt atttaacatt
    19321 accacctgtc caccacagga tggactttaa tgctgcaaac atgtgtttat ccttatatct
    19381 gttattatta ttaagatcct gatagccgca tagccatgtt ggcattacct tctcctgtgc
    19441 acctgcactc ttcctcacca tgtatgccac ggcaaaccct atgattataa taactatcat
    19501 cacaagagga acggcaacag catttgcata atgcataccc gggacattaa cggtaacacc
    19561 ataggcattt gtgaaaatat agttctgtgt ggatagctca gcaaatgcat gctgaataat
    19621 tgaacccgat gaggatttaa atattttaat aaaagtatca taataaacaa acggtattat
    19681 accctgaagt atacacaaaa ttgccagtac acatttgggc agcaacatct tccatgaaac
    19741 ttcatgaggt ttatggtcac cattccattc aacaccggtg gaggcaaacg acatcccaaa
    19801 gaatttaaca taacaggcca gggttaacgc actggtaaaa agagccatga tgccaaacaa
    19861 tacaaggaag aaaacctgac tgccccccag cagagttgcc gaaattatgg tccatttgct
    19921 tgcaaatccg ctaaaggggg gaactcctgc tattgacaac gatgcaattc cggctatgac
    19981 agcgctaatt ggcatgaatg tcataagtcc gcccaattta tttaagtctt ttgttcctgt
    20041 ctcgtacagt atgctaccgc tggtaaggaa caacaatccc ttaaatattg catggttaag
    20101 aacatgatac agaatgccaa tgatagcaac aaatgaaagc atctttacaa acatattgct
    20161 gctgtgatac atgtataaac ctgctcctat agccagaacg atgtatccta tctgcccaat
    20221 ggagctatag gcaagcaggc gttttgcatc gctttgcttt aacgattgcg aggtcccaat
    20281 aaataaggtt accacaccaa atgttgcaat aatagcgccc catacaagag gattgatagt
    20341 attaccatca tgaggtacca tccagaaaaa tgttcgtata attccatata tccctgtttt
    20401 aatcattaca ccacttaaca aggcgctgat tggggaagga gcaattgagt gagcatcagg
    20461 cagccacaac tgaccgaagg gaaaaacccc ggctttaaag ctaaatccaa gaaataacaa
    20521 tgtatagact attaccggca caatacccgt tgaagcgcca atcttctgag tcaatgtgtg
    20581 tattgaatcg cctgctgcag aaccattgat gataaatgca ccggtaacaa taaacaccca
    20641 tgccagctcc atcagtataa ggtatttatt ggcgttgcgt acattttctt tgtttttgta
    20701 ttcaaacctg atcaaaaaat atgaggcaat cgtcatgagc tgccatgcaa cggtaaaccc
    20761 aatgctgaga tcatcaacag taacgatgca caccattcct aaaataaata agggaaagtt
    20821 tataaagtag gggcgtaatg aatagtcttt gtaatgctcc atatagtcaa tggagtacag
    20881 tgcacttatt actgccataa aggcgataat ggcaataaaa aatcctgaaa aagcatctat
    20941 taaaaaatag acacagacac cgccaaaata tattgcctgt gtttcagagg attccataac
    21001 aaaaattgca ttatatgcta catagagcaa caaactgctt gcaattgcta taacaattgt
    21061 atttatccag cctgccagct ttcttttatt agctaaaagc ggcaccacta aacctgcacc
    21121 taataaaaca gccattgcct gtaataaaat accatacaca tcaatgttca tacacacact
    21181 ccttttggtt gctcattata gatgcttgca acatattcac tattgtttct gctttatcat
    21241 gaataacggg cgatagtccc tttccataat catcattgtt tataactatg cccagtaaat
    21301 agacatcctt ctgtgatgct tcccatattt tgcatgacat ctgtaatgct aatttatgcg
    21361 ttgatatatt caatcccgtc tccacaatgt catggagttt accaaaaacg atggtaccgg
    21421 gttctgcttc cccaacgatc gcatcaatga ttaacacatt gttgcaattt gactcagcta
    21481 tggtaaaaac atagctttca ataatatcct ctacattaaa cagcagaaca ccaggattac
    21541 tatctattgc ttttaaccta tcaagaataa aaagaccaac agcatcatca tggcgatagt
    21601 aattccctaa tcctacaatg catgttgtag tgttaaagat gttttgtagt gcagtaaaac
    21661 tcatatctgc tcacccaata tcttaatctg tcggtaggta taaacagggc catcagtgca
    21721 taccagggtc ctcccgatag cacagtgctg acactttccc acgccgcatt tcatgtgcct
    21781 ctctaatgtt gagattatca tatcatcctt aatgccgcgc tgcatgagct ctttaataac
    21841 tgcattaaac ataacaggag gtccgcatac aaatgctacc gtattctcaa cgggtatctc
    21901 caccttatta aacagtgttg ttataatacc cacattgcct ttccattcgg gtgtaccatt
    21961 atctacggta ataaatgttt caaagccttc tatcttctgc cattgctcca gcatatactg
    22021 atacatcaaa tcctgcggcg aacgcgagcc atataaaagc aaaatgcgcc cgaactcatt
    22081 tctgtgctgc aatacatgaa caatggaaga acgcagtgga ataagcccaa taccaccagc
    22141 aacatagata acattcttgc cttttatttc ttcaaagggg aacccattac caaaaggtcc
    22201 acgaacacca accttatctc caacctccag atcatggagg gcattggtta ccttgccaat
    22261 ctttcgcacg gttatatgaa atctgtcgtt ctcagggctt ggcggtagag aaacagcaaa
    22321 ctcgccggcg ccaaataccg tgcacatgat aaattgtccc gacttaatct taaaattgcc
    22381 atcaatttct ttattatcaa acctgagata aaatgttttc acgtcgacta tctcatcttt
    22441 aatttcttca atggtggcaa tttccggtac tgttacaatc ttctgttcgc tcataactat
    22501 cacctattca tgaatttctc ttcggatata tgtaacaaca ttaggcagcc caatatcacc
    22561 gggacatacc catgcacacc gcccgcaacc aacacatccc gggcgtagat atttctttga
    22621 ctgggaatat gacagtttgc agaagaatcg cttgtttctg cggtcttcta ttggtttccg
    22681 tggattgtgt cctccagcca ttcgcgaata tccttcatac gaacatgaat cccatgtgcg
    22741 cagccggtaa gtatcccctt caactgtgct ggtatcattg atattaaaac acgtacatgt
    22801 tgggcaaaca tacgcacatg caccgcattc tatacactgc ttaccaatat attcccatac
    22861 ctcttctttt ataaatcctg ttgttaccct gttcatcact cgagataccc atgcctttga
    22921 ctcaaccgcg atatcctcaa acatcgtgat ggacttctcc acaagcttct gcttcagttt
    22981 agcatgctca tcagttgccg ccggaaaatg gaaagcttca ataaatgcct ttcctttttc
    23041 actgccaacc tctgccaggt aactatcgcc cagatccgta agatttatat catacccttc
    23101 attagcagca ggaccgctgt cagtacatac acaaaaacac tgcttaaacg gtacaacaca
    23161 ggtactggaa acaataaaca tgcgactgcg atgatgacga tatacctcat caacaaattc
    23221 ctgccccaga aaaaacctgt caatgcactg caatcccgtt agatcacatg aacgcaatcc
    23281 aaataacgcc ttgggcgcat catcaaagag catggcaatc tcagctttct ttgccttttt
    23341 atcgtatttg tactgcatga tcttttctaa atgcgggaat acaacctgct tgggtggatt
    23401 ggttgggata ggagcattaa catttaccgt atccccgttg actgtatcaa agatacactg
    23461 tccatacact tcatcaagat atggaccata taacgtatac ttttcttcta aagcatgagc
    23521 aattttctta atatcgtcac gtttaatcag atacatatca caccttctca attcgtactg
    23581 ggattgttgc ttcttcgccc tgtgacacaa cgtcactata tgctaataaa aatgatgtta
    23641 tggaatccct tataaaatag ggagcatagg ctgtgcccgg tgcaacatca tcggatatca
    23701 taaccatcgc tttcatactt gccttttcgg caaccacctt caccggccat ctatcccgca
    23761 cgccaagctt ctttgcatcc tcaggagcta tcgccacata gccgtcgggg tataaaagca
    23821 gggttgcatt gtactcacgt aacggaataa atgtcttatg cattatagta ttctggtgcc
    23881 agtaatgttg tgctttgccc agcatgagaa gatacgggta actttcattt gcctgcacca
    23941 tggcgtatgg cttttgagga agaacaaacg ctggtttgtc tgttgcacaa tattttgtcc
    24001 cgtcagggtt tttctttgta cagggccatt gtatgccgtg tttttttgcc aatgctttat
    24061 ggcttaaggc ttcatagcat ggggtaagcg atgcaatctc atccatgatg tctgaagcat
    24121 gagcatagtt gaaaggtttc cccgctgcag cggcaatatc acttgccagc ttccacagtg
    24181 gtaaaacatc aaatgcagga ttgcattttt ttaccgaaag ctgtacacgg cgctctgcat
    24241 tggtaaatgt gccatcggtt tcaatatatg tagtaatggg taaaacaacg tgagcaaaat
    24301 cagctacctt attggtaaat gctcctatat aggcaacaaa gggtaatgat tttaatctat
    24361 cggcaaattt tataataccg tcatcatgat caacaataac gagagcttta agcggggatg
    24421 tgctgtcggc aagtaattct tcaacgctcc ttcctttcaa ttcaggaagt ttagtattcc
    24481 attgttgctc aaatcgtttt tttgccgcag tatcatctat gctctggaaa ccggtaagta
    24541 aatcaggagc gcaccccata tcaaagctgc cctgaagatt attgataccg gtaaccggat
    24601 ttactccgca accttccctg ccaattttat cggcaatggc aaaacagtta tagatagaag
    24661 ccaccgtgtc atactgtaat ccggatatac ccgaggtaaa aaaaacagcg gcgctttttg
    24721 cttttgctaa cttttcagca acaaacatga tatcacttac actaacgccg gcaacctctt
    24781 caatagtttt aaatgggaca gtctctaagt atctcctgta ttcatcatac ttcgcaatag
    24841 ttgttttccc ccacttttca ctgtcaatgt aatatgctat tgcatcaaac acctggcgca
    24901 tcgttccggg ttttatattc ataaacttct ttgatagcct ggcaatcttt gttgttcggc
    24961 tgtcgatagt taccagcaat gagccgttcc gcgcagcata gtgcaattcg ctgccaacga
    25021 tagggttctg tgttgttatg tccatgccag caacgatgat aacctctgcc ttgcgaatgt
    25081 cctcaagcga gcccagcatc ccagcacaac cagttccttt aaataaaaca tcaaggctgt
    25141 tacgatgccc tgattcagac tgaatactta tatggttggt tttgaatact gcacgggcta
    25201 acttcattaa tgcaaaatta tcctcattgg atgatcgcgg cgaaacataa aaagcaatct
    25261 cttccggctt ataagaggat aatgctgaaa caagtttagt aatagcatcc ctgtacgaca
    25321 cctcgccttc ttttacatgc ggttttgtaa tcctgtcatg agtggataat agctcatgaa
    25381 tattccagcc ccgtacacat aatctgcctt tgctgacggg gtgtactcgt gatgggaaaa
    25441 ccccattgac tgatgcgcca tctactttga ggtagtgacc gcatcctgtt ccacaaaagg
    25501 tgcacacacc tggcaaataa ctcatagatt atcctcgcta caattgtgag attgaaaaat
    25561 tatcaaaaca ttcacttcca gtcttttaac tggatacaat atggcacaat caataaccat
    25621 tgtaaatagg ggattttgtc ttttttttaa taggggaatt tgcttatgcg tatggaatat
    25681 atatgtttat tttaatttta gtaatttttt acaacatctt cccgaacttt atctcgtata
    25741 gaatcaagct cttcataatg ctttgcgcag tgttttcaat ttcctgcaac actctttacc
    25801 gggtgaacgt atactccatt gtatgtaatg ttaaattcaa taccgtcggc agcaagaata
    25861 tcacatatcc cttggagaat ggtaatctgc ccattttttg ctattttcac tgtacttttc
    25921 atcccttatt actttttatt cattcttttt ttttgttact accacgttgt catatacact
    25981 tatcaaaaca gcaatactac aatatccagc tttccttcta cccggatgag catataaaag
    26041 ttaaagttat gactaatttc aatctacctt ccatgcattt acttacatgt aaatatcagg
    26101 aaacatcata tcaatgaaat aacaataatt tcaatgcata atatgtcaca tggtatcgct
    26161 attgtcagac gattctactc tcctaaattg gtcggcccat gcagcaaatg aattttgatt
    26221 acgtgtctgt atccataatc ttcccgagcc actaaatcta cataccagtc cttcacctga
    26281 gaggaaaagt gactttagcc cacccacctt ctcaattgta aagctgactg atgggtcaaa
    26341 agcaacgata tgacctgtat caacgatgta ttctccctgc aattgaatct gatggatggc
    26401 gccaaagctt gaaaagaaaa gagtaccact gcctgatgct ttaagaaaaa agagcccttc
    26461 accaccaaaa aaagccctga aacctcccca tgtactatcg attgaaacgc tctgacttga
    26521 acatacaaat gcatgggttg ataatataat atcgtttccc gaaagctcta tcacctcaag
    26581 atcacccata gttgccgaag tcaataatat ttcacccgct ttatcagatg cagtaaaagt
    26641 gttaataaaa aacgattcac cgccaagaac agagcgctta agcgatttca taattccacc
    26701 acgagattca gttttaatct ccatgttagt agacattgcc accattgcac ccgattcagc
    26761 tactatggat tcacctgcat taagattaat cttcacacat ggaaaatcag gcttaccaag
    26821 tatttcgtat ttcattatta cctcctactc tctttttggc ggaagttttt cacgcaacca
    26881 tgaagtgaga ctgctaacat ttcgcgattg tacccagatt ttaccgctcc ccgtaaaatg
    26941 gcatacaagc ccttcaccac caaggaaaaa cgatttccaa tttcccaatt tttctattct
    27001 ataagtaagt gaatcttgaa acgctacaat gtgccctgtg tcaacaataa gactttcact
    27061 tacagtgagc tcctcaatac ccccatagct tgcaataagg agcgggccat caccgctgat
    27121 ttgcaaaaag aatagtgact ccccactaaa aaaacctttt aatccctgaa actttgtatt
    27181 tatctctaaa gagggactgg ctgcaagaaa tgaacttgac tgtacaaaaa ccgttccggc
    27241 aatatcaagt ttcttgatat caccaggcaa ggtaggagca agggtaacct cacctctcga
    27301 accctgcgca gtaaaagtgt ttagccagaa tgaggtacca ccaaaaaaag caatctttgc
    27361 tgatttcata ataccactgc tcgatgcctt ttttgtttct atggagacat tgacgctttt
    27421 acttaccata gcaccgtctt cagcttttat ctgctcatca gcattgagcg atacaacagc
    27481 aagtgcgtat gacggattat acttaatatt aatattcatg gctcacctcg ggagcagttt
    27541 agtaatccag ttaataaaac tattgggcac acgcgattgt atccacaatt ttcccgaacc
    27601 agcaaactca caaaccaacc cttcaccgga aaagaaagta gatttccaac caccaacctt
    27661 cttaaccgca aaagaaagag aatcttcaaa tgctacaata tgcccggtat ccaccacata
    27721 gttcccacta acaggaattt cgataattga accgtatgat gaaaaaaata aatcgccatt
    27781 accagacacc ctgagcaaaa atagcccttc tcctccaaac aaagaacgaa gaccaccaaa
    27841 ttgtggagta acagtcaact caggtgatga acacaggtac gaaccagatt gcagcatgat
    27901 tgcctcaccc aacatcttct tatgttgtat atcaccaaca tactgcggag caatgccaat
    27961 ttcaccacca ccatgagatg ctgtaaaaat atttacaaag agcgattctc caccaaagaa
    28021 ttttcgagca acagcagaca gaaatccact gccaaaacct gtctcaattg aaatttccgg
    28081 gctcatataa accattgcac ctgcctcagc tttgaccttt tcatctggag caagtttcaa
    28141 tgcaaggagt gtataggtag ggttgtgttt aaacacgtac tccatgcgaa cctcctttga
    28201 atcatgatat cattcctatc tttttataat gcctataagc tttataacct gcattattgt
    28261 gtatgtcaag tttttatggt atagctgtca cctcaatgcc caaaaagtat atcaatatct
    28321 taatccccgc catgctcacg aacccactga atagcatact gcaacaaatc ctgagatgag
    28381 tccagatgca atttcatctt tatcttttca cgaacatttt caacagtttt aacacttata
    28441 tgcaactttt ctgctatatg cttgttgccc agaccatgtc ctatcaactg aaatatctca
    28501 aactccctgt tggtaagacc ctccaccgag ctgtcaataa cacctctatc cgacaatgca
    28561 ttaactatca gctcttttac ctcacctgac aaataaatgt ccccttttaa aaccgtatag
    28621 atagcattga taatcttatc aggtgcttca ttcttcatta aatatcctcg tgcacctgct
    28681 ttaatggcac gttctgcata aagcttttca tcatacatgg aaagcaccaa tgcaggcaat
    28741 tttggaaagc gttgttttaa tgctttcacc aattctatgc cactggttcc atttaatgag
    28801 atatctacca gtacacaatc aggcaccctt ttattaatga catcaattgc ctcatcagcc
    28861 gaacgtgctt cgccaatgac ctctaaattt ttgtggcttg caataagctg ctgcaatccc
    28921 tgccgtacaa taggatgatc atcaaccaca actatgctga acttcttagt gcttttcagc
    28981 ttctcagtaa caggctttaa acttttatgt attgtgcact gaaccagcgt cccgcccgtt
    29041 ttattgttct gaatctgcaa tgcagcattg ataatccatg ccctgtattg catgatatgc
    29101 atccccatgc cttttgttac atcaacatca tctggtatgc cccgcccatc atccataatc
    29161 ctgatgcaat aatcattgtc atcctcgcat actgatacgg caatatgttt gggattacca
    29221 tgcttaactg cattgttcac agcctcacgg acaatatagt aaacatgtac cgtgtcaacc
    29281 aacgcatcaa tattgctggt cacatcccat tcaagaatgg ctttcatctt aaaatttttg
    29341 tcaatatctg cgataatctc ctgcagggaa acatgcaatc ctttgttgtt taaatcaacc
    29401 gggctcagcc cctgagcaat gaggcgggta tgttcaatag cctgatgcac catactctgt
    29461 atttctacaa tttcgggcac ctcattggaa aattcatctt ttaatttaag ccgcagcatc
    29521 tctgttttaa aaattatgcc tgcaagatgc tgccccaacc catcatgcaa atcccgcgct
    29581 atacggttgc gttcatcaat gtttgcctgt aaaatctttt tttccagctg cttcttttcg
    29641 gtcatatcaa tgccaatgag cataaaatag ttaaatacat cggcatcata gacggggcgg
    29701 gcatgccatt gcacaatgcg ctcggttgca tctttcatta ctattgaggt ttcaaaaatg
    29761 tgtgtggctt ttgttttttt catgtgcgat agttgatttc taaatgcacg ttgcccaaaa
    29821 tcagggataa acagcgtaca gcagcttttg ccggtaacct cgctggcttt gtatcctgta
    29881 gcctgcaaca tggctttatt gcaggtaata acagtaccct cttcgctcaa tgttaaaaag
    29941 taagctggtg ctatatcctc cagattttgt ataatgtcaa gtccctgctt taatttttcc
    30001 tttgtccgtt taacatcagt taaatcccgg gcaatgattg agcagcctat tatggtgccg
    30061 cgttcatcct caagcggggt tacggtcatt gacatattat ggattgcgcc atgcctgtct
    30121 accccttcag actcataatg agctatccct tcattatgtt taacccgttc aatgagctgt
    30181 tcaaattcat caggacggta tggcggcgac agcaagctta catgacagcc agctgcctca
    30241 tcaaacgcat agccaaagat tttttctgct gctttattcc agcttgcaat tgtcccatca
    30301 agagttgttc caataattgc atcatccgaa ttgtccagca aaatagatag ctgcgtgcgc
    30361 acctgttcta tctgttttcg ttcggttata tcacgggcta cgctcaacac tgcattttca
    30421 tcacgataat agattgttgt gctggcaatc tcaacgggga tggtgttgcc atggctatca
    30481 acaaaagctg tttcatacca atcggtattt tcagagatcg actgcaaaaa tgtatgtatc
    30541 cggttactat cgccctcaga ggtaagctgc tgcaacgtca tcatggcaag cgattcatgt
    30601 gaaaacccca aacggttgca tgttgtagta ttaacttcaa gaatgttccc atcacttttg
    30661 tggataataa ccatatccga tatgctgtta aacaatgact tataacgcat ctgcgatgct
    30721 aaaagctcac cggtcctttg cagcaccata tcttccagca gatccttttc tttcctgagc
    30781 atatcctcag cagattttat acgcatcatg gcatttatct gcgctatcag ctcggcctga
    30841 tctattggct tggttaaaaa tgcatcagct cctaatttaa gccccttaac cttactgtgt
    30901 gtatcagtat gtatggcggt aagaaaaata accggaatat ttcgtgtcat gggatcatct
    30961 ttcaaaatgc ggcatacatc aaagccatcc atccccggca tcttgatatc aagaataata
    31021 acatcaggat ttgattgctt tgccttttca attccctctt tgccatcctc agcagtaata
    31081 accatgcaat cagcaataag attttttaac aaagcagtaa tgcttaccag attatccttt
    31141 ttatcatcaa taaccaggat tgtactcatg attggctaac ccaatgctga acttttaaaa
    31201 caaattcttc tattggaaac ggcttctgta taaaatcatc acatcctgaa gccataatga
    31261 ctgcacgctc gtttttaagc gacgatgcgg taacgatgat aacgggtata tgttttgtat
    31321 ggtcatcgct tttcagctcc ttcaccacct ctaatccatg taaatccgga agcgatagat
    31381 caagcaatat acaatctggc tttacttcat gggcaagcgc tatacctctg gttcccgact
    31441 gggtttcggt aatatcgtat agttttgaca acagagcttt caccaccaca acgctgtccg
    31501 aatcatcatc tatcaccaaa atttttttgc gatctgcctg cggttttgct tccacctgta
    31561 atggcaactg caccgtaaag cacgaacctt tcccaagctg actttcaaca tggatggttc
    31621 cgccaaggat ggataataat ttcaatacta tggtaagccc cagccccgtc cctggatatt
    31681 ttgatgacag cgagcctgat gctttggtaa aacctgtaaa caatgtatcc agaaattcac
    31741 tgcgaatacc tattccggtg tcggctatgc ttactgtgca catcccgtca tgtgcatgcg
    31801 ctgacactgt tatacagcct tcctcggtaa acttcaccgc attatcaata aggtgctcaa
    31861 acacctgata caccctggtc tcatcactct gtagtataac atcatccaga tgcgtatgca
    31921 gcaaaagtcc ttttttctcc agtatacctt cataccgttc caaaatatcg ctcaatattg
    31981 ttgaaaccat aacatcagtt atatgcaact caatgccgct atctatctca aatacttcta
    32041 taaggtcatt taccagtgca aggaggcggc taccattgcg ttcaataatt gctaaatatt
    32101 ccttttcttc cggggtaagt ctgccatcag cctgcataag caaaaccttt gacagcgtta
    32161 atatggagtt taacggggag cgtaattcat ggcttatgct gtgcaaaaat tccttattca
    32221 tgggcttttt gctgaaaatt catttttatt aaatatgcaa tgttaaacaa atttgttatt
    32281 ccgccaacaa accgttcttt aatcatccct ttgggcaaga ggtcaattat cctgttatat
    32341 tcctccagcc tggtattgat tgtagcaaca atgttcgata gctcctgata ttctgatgtt
    32401 gcctgtaaat ttttcatggt gtcagcaaca gcagaagcgc taaaaaatat cttcctgaac
    32461 gcatctttta cagtcgcagg aaaccctgaa agaagcttct caaccagaca tatcctgacc
    32521 agttgatatc ctaactggac atcctgacag ttccggtttg aaaaacagtc cggaaaacgt
    32581 tcaaagtagg cattccccag tgcaatcata tcttcaatgt caaaatcaaa atttccgtca
    32641 caggcaataa tatcgcctct gaactcatcc agattttttg actttatgaa tagttccgcc
    32701 ggttttgtca taacattata ccagtccata aacaataact atcgcacaaa aactatagca
    32761 cataagcatt gttgttaata gttaaaatac cataccgttg cagcacactg ctacgatatt
    32821 tttatagtta aaatagcata gctttttttg taaagaagaa ttaatgccct actctctgat
    32881 tggcaaaaca agaaggctta acgggtttgc ctgcacacca tgaaccataa gcgaaacatg
    32941 aaggtgcggc cctgttgaca tgccggtgct tcccacagcg ccaataagct gtccggcaac
    33001 caccctgtcc ccggatgaaa ccatcagctt ttccatatgc atgtaatagc tgaatatgcc
    33061 gttgccatga ttgacgatga ccatgttgcc ttcgtaaaaa agtttatctg caagcaccac
    33121 aatgccgtca agcattgcat acaccgggtc accactgttg ccattcagat caataccgcg
    33181 gtggatacgc gtcaccgtct gccttttttt agtagagcta tcatacagca gatatcgtcg
    33241 ttttgcccag aatgctgaat tgatggtatg ggtattccgt ggatggtagg gaatgctttg
    33301 tatgcagtca tcaaaccttg ttgcaaatgc tttttgcttt ttctttgtgc attcagcaat
    33361 aaattgtttt acctcgggag tctctatgta atccttctgg gaatacttat caaactccat
    33421 tgggataacc gaaacgggaa aaaaagatcg cttaatagtt acaggaatct caatatgaaa
    33481 ggaatcacca tatatgatgc aatgataaac gcctggtttt aactcaggat caatagcaaa
    33541 aaatcctctg tacccccacg ttttttttga taatacaatc tctttatcaa taaatttcac
    33601 agaaaccaat tgtacatcct tagcaggtat gatttcacaa tacgccacat tgccctgtgc
    33661 aaattttttt gcatagatag tacaggtaac agcatcgtta ctgacagcaa ccttttccgg
    33721 catcactacc gtaaagttat ccggcaaagg ctttttggcg atagttacct ttgtaatatc
    33781 tcgtgcgcat gaaatggctg cacagcatac catgattatt gcagttactt gcatcttcat
    33841 gatgatagca ctccctctaa aaataaaatg gtgcacaacc agtttccctc tccttattca
    33901 aggagaggga tgaagggcga ggtttgtgaa cctgccacat actattttat cactatatta
    33961 ttgatttttt catgctaata ttgtataata ctatcatagc actataacca ttttcaacat
    34021 ctatttgtgg aggtcactat gagcatacag atttcattca gcaaactgga aaaagaattc
    34081 atgccgcaat tcagggaacg gatcactacc tctgaggata tcgttgatgt acagaagatt
    34141 ttttcatata ccataaaaga gatgttacaa aaagcttttg aaaaagaagg gattacaata
    34201 aacgaggaga atatagcact taccagcagc gcaccttact ataccataac cattaataat
    34261 cactcaataa atgaactatg gaacaccagt gacttaaaag acattttgca gcgctttgcc
    34321 gaggttgctc ataagcggta cattcatttg aaaaaaaata tggcaaaaac aaagatgaag
    34381 atacgtcggt aaagaattat actattttgc taatttttcc attacctttt ttcggtattc
    34441 ataatcagga acatgtatcc ctttttgttc catgagtatg gtataacgca gttgttttgc
    34501 attaagctgt tttacaagcc cttccagccg tttatcatca tcgcagcagg cgttgataag
    34561 ctgctgtaat gagtatatat cttttcgcag ttctacctct tcaggaatga tgcctgcatt
    34621 cttcattatt ttatatgcca tcctgatatc aggcggaata gtatccagtt catcccgtgg
    34681 aagaggtttc cccatccccg gaaggttatc aaactcacct cgctccatgg cgtcacggat
    34741 acgctgttca gctatcagtt caaagatgta catggatttc agtagccttt tttagaacgt
    34801 tcttaaaaaa aattctattg taaattattt cccattcagc aggggtatag atcaaacaat
    34861 caacaggcac acaaacgtat gactcaatgt cattgaagtc atcataccga tcaaaaaaac
    34921 gtttatccgt ttgtttgatg acaatcaagt caacatcact tttccctgtc tgtgtattac
    34981 gagcaaatgg accaaagagg ataaccctat caacaccctt tgacaaaaag ttatgctgta
    35041 attgcgatat aacatgttct ttaagcacca taacagtaaa gtaacgatat agtgacgatg
    35101 atgtcaatag tattttttta acaatatgcc gctataccgt aatccatcaa cctcttttgt
    35161 tcgataaata aaaaaaggaa gcgaaaaata tgaaacctct actctatata gtacctattc
    35221 ttatgctgtc acatcttgca tttgccaatg agtactggca gcgccagata gagtctttgc
    35281 aaaaagaaca gaaacaggtg cacaaacagc catacgcctc gtttgtgctt gaatatattg
    35341 atgctcgttt ggcaatggca caggaactgt cagccctgta ccacgaaagc ttaaacaata
    35401 aacacagtga gcacgccgca accacccatt atgtaaccgt tttatcccaa tgttttgatg
    35461 atgcagccat taacataaca cgacagcagg ttatacaaca gttacaacca ttgcaaacat
    35521 ctgatacaat agattgcacc accaatgact actacatcat tcataccgag ctggtgcttg
    35581 cctccataac cagctatacc agcacaggca tagaagaaaa agacaacgat accatgaaca
    35641 acgatatgtc cgcgccaggt gcaccgccta tggcaaacct gtcagcaagc gtcaggttta
    35701 acgagctata ccagaaggct tgccatacaa agcaaaaggc tgagtacata caatctattc
    35761 ttaccctgtg cagcggcgat acaacatcga ccgctcaata catgcacaat ccatcattgc
    35821 tgctgcagct gttcccatca cttgcagcaa aaatacatac aaaaactatc tatagcaatg
    35881 tttataactc cggcaatggc ataccatatc agataactat cccccacgaa atagatagcc
    35941 agaaagcagt aaacgacatt aacgcaaaac gagatgagct cattgcaacc agcactgtac
    36001 caaccagtga aattgacgca atagcacatg catacattga tccagtacat gatgccattg
    36061 ccgaacagca aaaacttctt gccaccatgc aaagcataaa cgggataacc attgaaaatg
    36121 aggaggcgct taacacattt attgagcatt ataccacgca atcgcgctat cttctggatt
    36181 atgcacaggc aaatacgctc tactgcaatc ttgtacagtt caaggaacca gcaattgatt
    36241 atcaaaagcg ctatgagcat attgcaacgc ttgcccatca attgcaacaa caaacaattg
    36301 cgggcgatag ctccggtaaa accatgcctg ctattgaacg cattgtttca atattaaagg
    36361 catttgcctg cacccagcat aaaaaagaaa acgatgctgc cgtacaaaac cttatgcagt
    36421 cactgcacac catcaaagaa gctatacgca gccgttgtac agcgcctgaa aataataacc
    36481 tgaaccctgc actctgcagc agggaaattg cagcaggcat tgaaacaata gaaaaaggca
    36541 tacacctttt tcagagccag cactatgcaa aagaggcttt gataaactat gcaacggtgg
    36601 ttaatgaagc atttgagcag gcttcaacag gttacggcag caacatactg cagcagatag
    36661 ttaccctgca aagtgccatt ccagcggtca gcaactttga tattcaaaaa atcatcactg
    36721 aatataccgg ccagtgttac ctgctgcgca aattgcgata cgactgtgca tcactggtgc
    36781 aacgattaca gttctttgaa aagcagggca ttgctatgcc tcaaaaagat aaagcctttc
    36841 agttgctgga aaccatgaaa accctgcaac ccctgtatag cgttgatgtt tacaaatata
    36901 caatgaatca gaacaatatc atcattattg accggcagtg tgcagcagcc ttaacaagga
    36961 tatcacaaaa aaataaaatg cttaccgcaa aggatatata aagcatacaa tgtcattgcg
    37021 ttattgtacg gtacagcagt cttgaatgaa taaataaata aaacaatagc agaaacaata
    37081 gtgtgtagca caacccaatg atatccccat ccattaaaaa ttgaataatt gcaacgcaca
    37141 taaaaaatat ttttacatgg ctgtcattgc ctgatatact tataagtaca ttgttgcaac
    37201 attaaggata attatgaaca tagctgtagc catgagcggt ggcgttgatt cctcagttgc
    37261 cgctgctctg cttgttaaag aaggacataa cgtcattgga cttactgcaa agattttact
    37321 gtgctcagag atggatggca gccagccccg ttttgatgtt tgctgcagcc cggaaaacct
    37381 gcatgatgca aaaaaggtag caaacagttt gggaatccat cattacactg ttaaccttga
    37441 agatgaattt tattgcaacg ttattgaacc tttctgcaac gaatattgca gcggcagaac
    37501 accaagcccc tgtatcaact gcaacacctc tataaaattt ccaaaactgt tgcaccatgc
    37561 aaaagaatta ggctgcgatg cgctggcaac aggacactat gcttccgtgt gtaaaaatag
    37621 ccgctattac attaaacgcg gaaaagatgc tgataaagac caatcgtact ttttatttaa
    37681 tctgaatcag gaaatacttg ctcatgtgtt gctgccactg gggctttaca caaaagaggc
    37741 aatcagggcg atagctcatg atatgggtct tcatgttgct cataagcccg aaagtcagga
    37801 ggtgtgtttt gttccggata acgactatcc ccgctatata gagcagcaca ttggcaaaag
    37861 gctgcctgct ggcgatatag taacaaccga cggaaaggtt ataggcaggc ataacggcat
    37921 tcaccggtat acaatagggc agcggcgggg acttggcatt gcatggcaac atcctctcta
    37981 tgtcgtggca attgatgctg caaacaatcg cattattgtg ggaagcaacg atgagctttt
    38041 atccagcgga cttattgctt caggcataaa ctttatgaag gcaaccgcat taaacaatac
    38101 accggtatgg ataaaaacac gctccacgca aaaaccatgt aaagctactg caacaacaga
    38161 taatggcagg ctcatcataa cctttgacca gcctcagagc agtataactc caggacaggc
    38221 ggtggtttgc tatgatgacg acggtgcaat actggcaggc gggtggataa aggaaaaact
    38281 atagagtatt atttatctta ctattaaaag tacttttaag gatactattg tgattatcag
    38341 agcaaatgat ataaaaacaa aaggggttct tttgtttgaa aagctaatag cgccaggtga
    38401 ggatatcatt attaatgtac ggggcaaaaa taaatatgtg gtgctggaca ttgaacgcta
    38461 caaacaattg cgtgaattag aacttgatac agcttatgaa aaagttatgg atgatataaa
    38521 agctggcaac tataaaaaac aaaccgccag agagcatgtt gatgatatca taaatgaact
    38581 atacaataat aataacagag acataccgta aaaaagtcat taaattcttc aggaaacacc
    38641 gtgaaatgat acatacgtat gcaaaagtat tatctattct ggaaacaaat ccctatcatc
    38701 catcattacg actgcataaa ttacagggaa aattacatga atactattcc atatcattgg
    38761 atacacagta caggatgctg attgtattca atattgatga aagcaccatc atacccattg
    38821 atattggaac gcatgatgaa atatagtgaa agtatattga catactatca caacggtaat
    38881 ggcaaacatt ttaataatac tccctgtgcc agtcagcact tatacggatt atagtccttt
    38941 acaaaatgct gtataaggct tttgcattct acagcgctta agctttgaac atggtcagcc
    39001 tttaatgata ccacctcgtt aaccagctcc ttatcaacac cattgtgcgc tattgtataa
    39061 attttagtat gcgctgttgg cacaagctct tcgccctcat tccataattc ttcctgcagc
    39121 ttttcaccgg ggcgcactcc tgtatataca atagggatat caacatcagg ctggtatcct
    39181 aaaagtgcaa tcaaattgcg cgccacctct tcaacacgat actgttttcc catatcaaga
    39241 acaaaaatct ccccgccctg tgaaagcacc gttgcattga taacaagcat caccgcctcc
    39301 ggtatgctca taaaatagcg cgtcatatca ggatgggtaa tggttaccgg acctcctttt
    39361 gcaatctgct tataaaacaa cggtataacc gagccgcggc tgccaatgac attgccaaag
    39421 cgcacacagc atgtacgcat actgttgtta taagccagcg taaccatctc ggcaatacgc
    39481 ttggttgcgc ccatgacgct taccggatgc accgccttat cagtagagat acatacaaac
    39541 tgtttaacgc cagcatgctt tgcagcttcc agcaccgtta ctgttccaag acaattgttg
    39601 cgcacagctt cctgaggatt gtgctccatg atatcaacat atttatgcgc agcagcatgg
    39661 aataccacat caggcttttc gttatcaaga atataccgca ttcgcgttgc atcattgatg
    39721 tcggctatat agtatcggat gtcaggctta tcaaaatatt ttgcataatc attcagcatc
    39781 aatgttaaat tatatattga atgctcaccc ctgcccaccg ctacaatagt ttttgctcta
    39841 aaatgtaaaa gctgcctgca cgcctcagag cctatacttc cgcctgcacc ggtaacaagc
    39901 accaccttac cggcaaagaa ttgctcaata gcattgctgt caacctgtat ttcctggcgc
    39961 cctatcagct cttcaacgcc aataccctta agctcggggg tcagagcctt ttcaaacaga
    40021 cggctgacag ccggaagtat ggatatggta acaggatgat cacagctctg gataatgcta
    40081 ataatcctat ccacagcctt ctttggtgcc gacggcatgg caatgatgca ctcttttact
    40141 gtgtactgct gcaccagcgc cggaatatca ccggttgtgc caagcacagg tttgccgctt
    40201 aaaagcgtgc caaccttttt tttatcatca tcaacaaatc ctataatgct gtcatacctg
    40261 ccattgcgct taaactcggt tgctatggtt tgtccagctt caccggcgcc ggcaatgagg
    40321 ataacagatt ttttacccgg ggcgatagtt actgaagatg aggttagctc ccttaaaact
    40381 atcctgtatg caattaacaa aacaaagcct gtgccacatg caataaacag ggtggcagca
    40441 tccggaatag ttatgccata ggcagcgata agcagcagta caccgcccag cacaaagcct
    40501 tcgccgacac ggtaaaaatc ccgtatggta gcataccgcc atactatgcg atagcactgt
    40561 gccacatata atcccgctat cgtacatacc gcatgaactg ctgcaacctt catcacagca
    40621 tatcccatcc cgtgcagcag catggacgcc atcacatacc ctgcaataac gcatgctatg
    40681 tccatcataa ccaacggcat ctgcgaaagc cgtatactgt agaatacctt tttcatggat
    40741 tatcgtttat agtgttgtgc aatatcaata acattgtcta taacataggc aatctcttca
    40801 tccttcatag agggatagat ggggaggctt atttcacgct caaacaccca ctccgcagaa
    40861 ggaaatgtac ggtaactatc gcccagcctt tttttaaaat acgtaaagcg gtacaatgga
    40921 ataaaatgca cgcttgtgtt tataccgcgc tctgcaagnn nnnnnnnnnn nnnnnnnnnn
    40981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    41041 nnnnnnnnnn nnnnnnnntc atgttatatt tatatccctg ctgttccaca tcgtagtacc
    41101 agcttccatg attatcatac cgcttccatg catcgcgatt catcccatgc aggcgcagcg
    41161 agcgcgcctg gtcagcccac ctgttgttag cggtcacaag cataccaccc tcaccggttg
    41221 acagcgtttt ggttgcataa aagctatagc aaccgcaatg ccccaccgtg cctgccagct
    41281 tccctttata atacgatggc aatgaatggg cagcatcctc aatgacatag caattgtgcg
    41341 cttttgcact atccatgatt gcatccatat cgcaggggtg tcctgcatag tgtaccggta
    41401 taattgcttt ggtcttaggc gatagtaatt gcttaactga ctttacactt atacaatgtg
    41461 tttctctgtc aatatctgcc ataacaacag tggctccgca gtattccacc acctcagctg
    41521 tagcaacaaa ggtatttgca ggaactatga cctcatcgtc cttgcccaca ccacatacct
    41581 ttaaagctaa gtgaagcgcc gccgtgcacg agcttaccgc aacggcattg ggcacattga
    41641 cgtagcgagc aaaggcgtct tcaaatgcaa tggttttggg acccatggta gtccatccgc
    41701 ttttaatggc acctacggca gcatcaatat catcatcatc aatatagggg acatggaacg
    41761 ggatgttcat ggaagtatat ccttatgcac aacatcgtat tatttctatt ttcggataaa
    41821 agcggcggta agaatatcaa aagattttta ttttcaatga caactatcac aaaaaccaca
    41881 ggaatgtatg cttgccagag ccatcttttt ctgtatattt atacaaaccc ttcagaatgg
    41941 aaaacttggt aaaacccgtt tcttcttcct tttcatatgc ccataatata ccccacaaac
    42001 tgccaactga atggtatttc cgttcaggct tttttgtttc atcaaaaatt gttcccagaa
    42061 gaaccatccg cctgccatca ttcattaaca agtcttcatt gcgataatac aacaagccta
    42121 aaagccccag atcaacaatc ttataataat gtcggtaaca tccactgagc aaaacaggta
    42181 taatcaatag attgaattga tgttcactat agtaataata cagaggaagt atcttatgac
    42241 tgaatgaata gttatcctcc tctacaccaa caagccacaa cagagcatca acatcataca
    42301 tgggtttgtt tttgtcccgg tcatattcaa acaatgcacc ccagagcagt cgtaagcgta
    42361 gtacttcggg ttctgtggtt actgtcatag ttgtcaataa caatgaatgt tgcgtcttaa
    42421 tggcttcccc tgaagaataa tagtttttat aatcatataa aaaaagcata gtatcccttg
    42481 tatagctctt tgatcggtag cggtaatacc ctgcaacaaa aagtgactga ccatcatcat
    42541 cacgatgttg ccacatcaaa cacagcggga tggcaaatgt atcatcatgt ttatccattg
    42601 tgttgacgct tttgtagtat aacggattta tataaagact tttagcaatc aagccattgt
    42661 tactatattc tttgttgtaa tgaataagtg agaatgacct tgtaacctta taaccatctt
    42721 ctgtatacac actaaaccag taaaaaggaa aaatcctgaa cccgcgctta gtgtctgaat
    42781 gataccaatt gatgaaaggc catagtattg ttttttcatc atagcccgca gtatcatatt
    42841 catgccaata gcaattgata agatacatgc tggcaaaagc attgttatcc ctttgatatc
    42901 cgtaaaaagg aaataaaaca aaatattctt cagcatccga ataatatgaa acatacaatg
    42961 gtagcaaaac aaacagcttg tcttttgaag cttcatccca ggtaagccat gaaagtggaa
    43021 gcaatctaaa atgtcgcatg ctatcagctt tttcaaatgc cattaaacca aaaaatatct
    43081 tccaacccca gaagaactgt gaatcttccc tgttaacggt cttcttttta ttaatctgtg
    43141 gatgcgttgt atcactacca ttcaaatgat tattggcgat agttctcttc atcaaggcac
    43201 tgtcagcatc atagtctgct ggtttattat tatcaacagc atcagcattt tgcaaataac
    43261 tatcgcccaa acctgtattt gctttatccc gtgaatatgg aatacgagat gaaaagctcc
    43321 acattgcata taaccacgat acatcagtat caagatattt tctcccttca gattgggaaa
    43381 gattaacccc aacatctggc ttaaaaatac cagtataggt tttacgcgca taccctgtaa
    43441 ggttaatatg aacagtagtg ttggtatcac tatagttgta aacaaaaggc agtagtatat
    43501 tccctttgct tgtatcatta cgccatgaaa aatagatggg caaaaaatga atatagtatt
    43561 gctcaacacc gtttttttga ggcgcattat ttatggcata gtgcttgcta tggtaataca
    43621 accatatcca tagcctatcc atatccggct gccagcggtg aaaatgaaat aagccaaagg
    43681 aaacaccctc ataagctgtc ctgcgtggac tgcagtataa tggcacatag tagcggtatc
    43741 cattatcagt aaattcgtga aacagcaatg gcacaatcca cagggtgtgt aatgcattat
    43801 ggtttcttct ccaatcaatc aaccatagta tattggtatg tgagctatca tcacctgcac
    43861 tatgataata tacagggata accggaaacc agatgttgtt gtaggtatag tgttgtgtgc
    43921 ttgtgtcata aacagtgcgc ctgccaaaaa gtggtgttac cgtatagtgt tcgatagtag
    43981 cctttgataa gtctttaccc cacataaata ccggcaaaca ccataaacgc tgcaacccgt
    44041 gttcatccca gctataatcc agcaaccata caatattacg gtgtccatag ttgttgcgca
    44101 catggtggta agtgagcggt attattggtg cccaccatgt atactcacta tcatgaggag
    44161 tactatggct actataccaa aaaagtgggt tgccatacca tgtttgtgac agttcatccg
    44221 aacggtatac acggtaatat gacagcaatg gaactatgac attgtactga gaggtaaact
    44281 gctggta
//
LOCUS       DAOOJH010000013        43324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_992_length_43324_cov_9.676258, whole genome shotgun sequence.
ACCESSION   DAOOJH010000013 DAOOJH010000000
VERSION     DAOOJH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 43324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_992_length_43324_cov_9.676258"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..556
                     /gene="tatA"
                     /locus_tag="PLJ75_03375"
     CDS             389..556
                     /gene="tatA"
                     /locus_tag="PLJ75_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPD04289.1"
                     /translation="MPNLGFGELVIIFFIVIIVFGAGKIPKIAKDIGSGIKEFKKALN
                     SDDDVKDEKKS"
     gene            984..2018
                     /gene="rlmN"
                     /locus_tag="PLJ75_03380"
     CDS             984..2018
                     /gene="rlmN"
                     /locus_tag="PLJ75_03380"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPD04290.1"
                     /translation="MKKYIKNFSLEEVEQIFASINEKSYRAKQLFNWLYERNVDNFNT
                     MTNFSKKLRQYLTDNYEISALQLIDRKKSNCDGTEKFLFATHDNHFIESVFIRNDGTD
                     EGRLTICVSSQIGCPMGCTFCETAKIGFKRNLECAEILDQINKVRQLTGLKNNNIVFM
                     GMGEPFLNYDNVMKAAAIMNYTFGFHISVRKITISTCGIYEGIQRFIDEKMPYNLAIS
                     LNDTDMVKRSRYMPVEKKYPMQNIIRLLDVKFPVSRNRVTIEYVMRKDNISEEDAQRL
                     KSMFKYNRIKLNLIPLNKGKHNMEIPVEEEINKFIKRLEIMNIPISIRKSAGSDIDGA
                     CGQLSGRHYL"
     gene            2049..4304
                     /locus_tag="PLJ75_03385"
     CDS             2049..4304
                     /locus_tag="PLJ75_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD04291.1"
                     /translation="MITDIIAWIREHKVFTFVGIPLICCFLIILYTSVVYIEWLHDKQ
                     NALDKLFKYKQLIDRTEELKGGYSYTYSEIDVSAKVVDIPTRIYDRNNEIIGEFFEQK
                     REIVPYSYFPKWLIKAVIASEDRNFYNHKGVNYKGIARAFITNIIHFRVVQGGSTITQ
                     QLAKVLFTDMERSIKRKIYEIFCAFEIEKHYDKQDILSMYLNYIYFGNGAYGVEAASK
                     MFFGKSIVDCNETECAMIVATISNPLIYSPLNNLDLSLKKTKRIMQSMIDAGFISSDQ
                     AQLQYRNFIKQWEVKFSKDTIAESSLIGSFIFSLYRINRAPFFNEYIRRILVNKFSDE
                     VVKKGGLTVYTTIDAKKQDIAITALRKGIQLQREYHEKIAKALKNPDRALLEMEKANN
                     IEGAFVSLNPFTGEIISYAGGYNFSYQNQLDHVQQIRRQPGSSFKPIIYVAAIQDKVI
                     TPSSVFDDIPMTFKGGYAPKNYDGFYSGKIIIREALKKSVNIVAVQVLEKEGYSRVMS
                     IIQKGLNLTNSELDSRFYKTLSLALGTYELSPLENAVLHSVIVNGGDFVVPYGIRYVN
                     DYNGNIVWNNEEEVLLQIQEKRKEFGKIIEPSACAIVVSMLKGVFEEGGTASWILKNR
                     STPVEIAGKTGTSSDFVDAWFIGYTPDSVSAVWIGNKAGAISLGRGRSGGVLSAPVWA
                     DYIFAAYKDAEYKSFSIPADDITKQTICLDTGLVPRGEGWCPRIAKDELFYAGTEPGE
                     YCTMHTVPVLK"
     gene            4315..5181
                     /locus_tag="PLJ75_03390"
     CDS             4315..5181
                     /locus_tag="PLJ75_03390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04292.1"
                     /translation="MTKSIHLSVTIAIIVVSLLSVSFQLPVQNGTIHSTFGEPRSDHF
                     HNGVDISSIDKKVYPVKDGKLIFLWDKSLFPLEQYPGVGNYCILEHNKQEKSLYVHLD
                     NGISMSDNYTAGDSFAIMGNTGSSYGTHLHFGIIKNGRISINPFVVLPHVDDVKTPVI
                     EAIYIKVEDKYTNIHEGSTIRLTKHHPIAIQCFDAVKKNERCGIYKLKVIVNNDIVAD
                     YKFDSIVLNDKNKLTIDGFAFENLYDAKGNYLVWSINYKQGINTINVIAEDFAGNQTS
                     KSISFMVVLDFK"
     gene            5224..5628
                     /locus_tag="PLJ75_03395"
     CDS             5224..5628
                     /locus_tag="PLJ75_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD04293.1"
                     /translation="MILFFRRKKNIKQVDMAKALNVSPSYLCKIERGVQEPTEEFKRM
                     CARYLNISVSKLFPERVKNSKDVINDIVTAKNKIWNIRMAKGIKQYELAKLLHCSPSY
                     LSKVEKGLQEPTEEFKKVCAKVLKVKEIELFT"
     gene            5652..6905
                     /locus_tag="PLJ75_03400"
     CDS             5652..6905
                     /locus_tag="PLJ75_03400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPD04294.1"
                     /translation="MTLSIHLQFVIILVCLFFSAFFSGSETVLFSFKKSELIRFSFSE
                     NKLENQIAKFLSYPHNILITILTGNLFVNILISSLSTSFLLTVWPQYGHIIAILFVTP
                     VIILFVEILPKIVSLYSYKTIVYYIMPAILFFHKVLLPVRYVLLFISNTFINVFKLRI
                     DSSFDISEDDLSMAIYYGEKFGFLSQDEKQFIHNLLRFTKKTAENAMIHRSKTIFIPY
                     NATISEVISIVKETGIVRAPVYKNNLDTIVGLIDSRDIVSYATGFKKGTSIKKIIYPI
                     YHYPASRALGELLEEFLRNKIQIAVIVDEYGGTLGIVTLKRIIAELFGRGYTLWEDTP
                     KPEIKKEKGAVIIPGDMQIDEFNMMFNVSIESSESETIGGYIIEKLGYLPKRRASIMI
                     DDYIVTVRYVAKNRIQSIEVKRAES"
     gene            6905..8173
                     /locus_tag="PLJ75_03405"
     CDS             6905..8173
                     /locus_tag="PLJ75_03405"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPD04295.1"
                     /translation="MNIYIGTQIIILIFILFSAFFSATETALVSANVLYIQNLINKGN
                     KKAKDILYLIENPDKALTMLLIGNNIANIGATAFITFFASRALLIHDSKLFIVTIVQT
                     IFFLIFCELFPKIYSRSKSEKILFFNYWLIKIFNILFMPLISLIIFITDFVKKIININ
                     DTQLLPISSRDEIDTLFRAVEKEGIIDSQHYIYFNEILSFKDVRANEVMTPLIDIISI
                     EKNQSIKLCITLIEKTRFSRIPVFEERVDNIVGYIYYQDILRNPLIKNLQQIMHPPHF
                     VPSTKKIYDLIKEMEANNVAMVFVVNEYGAVEGLLTDEDIAEEIVGEIQTRDHPQDES
                     IKEIAPWKYSIAGNLDIEFFQRRFGIKIEKHDFETVAGFLLYQMGYIPKPGEKIRYMD
                     ITFQIERATDRAIERVIVTIPKRKTTIAQA"
     gene            complement(8157..8501)
                     /locus_tag="PLJ75_03410"
     CDS             complement(8157..8501)
                     /locus_tag="PLJ75_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPD04296.1"
                     /translation="MGCLFCDIITGKTPSNKVFENDKVYAFRDISPQAPFHVLVVHKE
                     HIKNIDELTPKNSYIINDIFLAIKKIAEQEKLDKGYRVIINNGSAAGQVIWHLHVHIL
                     SGKDDLGPMLVQ"
     gene            8597..9727
                     /locus_tag="PLJ75_03415"
     CDS             8597..9727
                     /locus_tag="PLJ75_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04297.1"
                     /translation="MRTINEIQYLVYPHKNINDEIVKRFYSTAIPHKIPYKNEDDLVL
                     IVNELKDSCIDSKDVIIIKPFLGRFYQVCPGSPNMICCRYRVINTCFNCLYNCTYCYM
                     HIYLNSYGIIQFNNTDRLFAELNNFVTTCSSSMVYRIGTGEFTDSLMMDEITNLGRQL
                     IQFFSVHDTIFFELKTKSSSVDHLLDVQTKGNAVLAWSLNTSANIALYEHGTASLTER
                     INAAVKAIDAGYYIAFHFDPIIMYDNWEEDYYNIIALLKQYINPQRVVWISLGGFRYH
                     LHFREILRNKFNHEMMTTGEMFPGRDGKYRYFKPIRVKMYHFMYKHLQELFHKTYIYL
                     CMETDDVWHQITGKYFNSAEELESDMANHLYKHFVQKKTILD"
     gene            9816..10931
                     /gene="prfB"
                     /locus_tag="PLJ75_03420"
     CDS             9816..10931
                     /gene="prfB"
                     /locus_tag="PLJ75_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_120190971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPD04298.1"
                     /translation="MIETKDLLRELSQIKNDIGELYTTLNIDDKKKQLSLLIEETFKE
                     NFWQDQAHCTQIQQQITQFKRTIEPLVALKQEVEDSIVLLEMAIDEKDNDVMKDIGSK
                     IEQFRNRFEQLETYELLSGADDDKNAIMTIHPGAGGTESQDWASMLMRMYMRWAEDND
                     FEVEVLDYMPDIEAGIKEATLLIKGQYAYGFLKGESGIHRLVRISPFDSNQRRHTSFA
                     SVDVIPEAPDNIDIDIKESDLRIDTYRASGSGGQHVNRTDSAVRITHIPTGIVAQCQN
                     ERSQHKNKAYAMKILKSRLYERKRQELEKEKMEKFGEKKDIAWGNQIRSYVFHPYTMV
                     KDHRTGEETGSVEYVMDGEINKFIYAYLKSLKNINRK"
     gene            11179..12387
                     /locus_tag="PLJ75_03425"
     CDS             11179..12387
                     /locus_tag="PLJ75_03425"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPD04299.1"
                     /translation="MINYKKYILYTLSLLLLILFIIINAINTIFAAGSGGTSGADFLE
                     IGVGSRPLGMGEAFTAEVNDINAIYYNPAGLATLKFPVMSIMHQELILDSRFENISLA
                     LPLYGGFFGFSTSVFWVPPFDKIDINGNKVGTVTFYNVSGILSYGYSLGFMEIGGSFK
                     YIYQKIDTLQLHSVAFDVGILKRITMYSPFDAPIKNLSFGLSVQNIGTNANDDSLPRL
                     IRGGVSYKMSHFFGINVDFIENAINSSDLYDFTSGFNESFRVNTGIELNYLDILFFRT
                     GYRFNDAGTYTFGMGFNYAIKNVAFVIDASYQDAGVFGPIYSFTVTFKLIPRVITIED
                     KLKAEAHYQRGIKYFITDDIDGALEEFKKASDYDPYHKNVDKKIKDLEELKELKRQNE
                     LLEKEQKTVQ"
     gene            12350..12556
                     /locus_tag="PLJ75_03430"
     CDS             12350..12556
                     /locus_tag="PLJ75_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04300.1"
                     /translation="MSYLRKNKRQYNEMREVTVYTDYCPNALGSVLYQCGNTQVICAV
                     SCSNQVPQHALDKGTGWLTAEYNG"
     gene            12571..13092
                     /gene="rph"
                     /locus_tag="PLJ75_03435"
     CDS             12571..13092
                     /gene="rph"
                     /locus_tag="PLJ75_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPD04301.1"
                     /translation="MLPYATVPRTERNRIKPDGRSVEIQRLIGRCLRTVVDLTALQNI
                     ALVVDCDVLQADGGTRTAAITGAYIALQIAIQKLIHRNVIDVNPVIAMVAAISAGYIG
                     DSLLLDLDYAEDSQCDVDMNLVMDSNYQIIEIQGAGEKRSYAFHELDSMITIAKKGIE
                     KLFQIQNEILKLC"
     gene            13159..13890
                     /locus_tag="PLJ75_03440"
     CDS             13159..13890
                     /locus_tag="PLJ75_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04302.1"
                     /translation="MQKLIMIIMCILCFDVAYAQYYVRNTTVIDTPTAYTIARGTYQL
                     SFLGYDNGGVELKAFLGLHNNFYLGASFDCQSAIGKDDPDPNVPGVVGKIKFTDGWEM
                     FPISFAIGYDSFYIGEEGKTYNSKNDLDQVIYGPYFVITKPIYLFDDEQHIHFGMRMP
                     TQPDYVPEDTSYFLSFDIPLGDMFVFKAEMERVYYNFSNPEDWLYNVGMRYSYMQRLG
                     IEFDVLFQKHENPNRIIRIEYINEF"
     gene            13981..14424
                     /locus_tag="PLJ75_03445"
     CDS             13981..14424
                     /locus_tag="PLJ75_03445"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPD04303.1"
                     /translation="MIYGYAFLTNHSGYIYANDKRHCIITIVNTLNEKIQCNVEIALT
                     DEEHATGLMNRTALCDDCGMLFVFADETYRTFWMKDTAIPLSIAFIDANGIINDMHDM
                     NPFQTFPEYSSKYPARYALEVNKGWFKKNAISVGSKVIMHGCISK"
     gene            14405..15577
                     /gene="hflX"
                     /locus_tag="PLJ75_03450"
     CDS             14405..15577
                     /gene="hflX"
                     /locus_tag="PLJ75_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPD04304.1"
                     /translation="MDASVNRIPISEKERAILIGVLLPEADESEISHSLDELTQLVHT
                     AGAVVVTRYIVKRMAFDPAYLIGMGKLTEIKNLIAEQSIKLVVFDLNTIRPVQIRNLE
                     NYLHCRVIGRTEVILDIFARRARTTEAKIQVELAQLNYILPRLKGLGGVLSRLGGGIG
                     TRGPGEKMLETDRRHILRRITTLKKKLIHISHRRDLQRQQRLHNEIIGAVVGYTNAGK
                     STLVNILAKDDLFVEDRLFATLDPYTRSVYLDNQHNVLLTDTVGFINNLPSHLIESFK
                     STLEEIKYSDFIIHVVGLDINIDRAVAVVAHELQLLDSNNKPNILFFNKCDIVSPDVI
                     HHVRLLYPDSIIGSCLTGEGIINLKERMIKIIETTHHKKQQSIINKNIELKYHHLN"
     gene            15659..16834
                     /locus_tag="PLJ75_03455"
     CDS             15659..16834
                     /locus_tag="PLJ75_03455"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04305.1"
                     /translation="MYDFKRYTFPEKRKINNSIYGFFSILFILVICIFTLIPGKSNNK
                     GISAASNLNSVENKLIIDGVKMEDDMSPAETEGEIVYDDSFDDDIDINNIVTIEHNDK
                     KIDKKDDKKKEKSFDIEKLKNNDKRWHLSKYTIKKNDNIWKIAKKFNVNYKLIIMANE
                     IDNPDLLKPGKTIMVPNKNGVLYQVKKGESLYVIARQYNVSPDRIIKHNELKNANKVL
                     SGQKIFIPDAVEVIKKNPGDVLRDNKSIAKNNVTGNINKSNNINLSLLWPVQGKITSG
                     FGTRRNPLGKGKQFHCGLDISCEPGTTVKAALDGNVIFSGWKDGYGNVIILKHDKGYI
                     TVYGHNSNNLVVEGDTVEKGQEIAKSGMTGSVTGAHLHFEIRKYLTPLNPLLYFKEK"
     gene            16931..17914
                     /locus_tag="PLJ75_03460"
     CDS             16931..17914
                     /locus_tag="PLJ75_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04306.1"
                     /translation="MKYDNPNQIIKIFYTQNDYVAWKAIVKGRILSIGVREDAAKQEL
                     TRTVQPKTKITARLYNTEGIHKGTTLFIINSNNLIVSRFTVENIFHSQSFGDMLIGYG
                     YFRLSTIGDRVVQRFDGAYPEYAKVHKARGDYYVNIGDYGNAIKEYNEAIIMDMNYPE
                     THLALGKLYYNDNVYQFAFTEFEQAYKNKDRMVDKQDYYELLYLMAKTRYIEAYETNI
                     PKKLKEHYLKEGMKYANEALSINPEAVELLYMLGVMYYKSPEPDDVAAKKTFEKIIEL
                     DPTHIDAYIALSELYLKHKNKDKASYYSQKALTIDPANERARFLYRMTKEK"
     gene            18106..18891
                     /locus_tag="PLJ75_03465"
     CDS             18106..18891
                     /locus_tag="PLJ75_03465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPD04307.1"
                     /translation="MITVMQNEIVQSLLPLIIINAITLLSCIIFIFIYPRRKKDPEIA
                     SRMHETFLGLFFREYWYWLNKPFINFFIYFKIKPNAITTLSLITAFISAYYFYSGNFG
                     LAGWILIVSATLDIIDGRVARSTNTVTKSGAYWDSCADRYSEGVVLLGIALYYQNNFI
                     ACLATIATLIGSELVSYTKARGEAIGITTTRGVMQRAERLFTLCIVSVLHPFFQVLFK
                     NTNVNPEIVMISAMILMAVATNLTAITRLRTIFMEIKRQENNA"
     gene            18884..20167
                     /gene="mtaB"
                     /locus_tag="PLJ75_03470"
     CDS             18884..20167
                     /gene="mtaB"
                     /locus_tag="PLJ75_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPD04308.1"
                     /translation="MHEKTFSIKTLGCKCNQYESAQIASQLLASGFTAVPFGSLADIV
                     IINTCTVTDKSNKKCRNYIRQGARFSKSGGVIVTGCMVQTHKHEIETMDEVIAAYTNE
                     QKGQLISLLGTIDNTKQKSLNHFETKTSPFALPYMRTRGYIKIQDGCDGHCSYCIVPA
                     VRGVPQSRPIDDVINHARYLIDSQCPEIVLTGITIGKYHDKGYSLAQLIEKIVQLDGK
                     FRVRVTSVEPVHVDDGLLAMLSHHKVCNHIHLPLQSGSDAVLKHMRRPYNFTYYMHCV
                     EKMKRVLPDIAIGTDIIVGYPVEGDSDFALTLQAVTECQFAYIHQFTYSPREGTCSSE
                     CKTIPHDIVAMRAEQLRNAAQKCSLQYKKQFIHATREAVIEKGNDSIVALTDNYLKVK
                     LESNHHTRSKVGQLLPVTITTNSSTGLQGKIACDN"
     gene            complement(20221..21159)
                     /locus_tag="PLJ75_03475"
     CDS             complement(20221..21159)
                     /locus_tag="PLJ75_03475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HPD04309.1"
                     /translation="MHKINNKIQLPAHSLIAAIGNTPLIELTKLSPSNTTILAKFEGT
                     NPSGSIKDRTAWYMIKSAIESGRLTHDKIILEPTSGNTGIGLAMVGSVLGFQVTLCMP
                     SCVSVERQHTLIAFGAHLILTDGCMGTDGAIIQAQKMCMEDPTYFMPNQFSNPANVMA
                     HYETTGVEILQQTAGAIDAFVAGLGTTGTIMGVARRLKEHNSSIKIIGVEPPPGHSIQ
                     GLKNMQEAIVPEIYDPGLLDDIITIDDETAFEYSRKLALQEGLFCGMSSGAAVAGAVK
                     VAQGMKHGNVVTILPDRGDRYLSTSLFQSICAKCPP"
     gene            complement(21335..21406)
                     /locus_tag="PLJ75_03480"
     tRNA            complement(21335..21406)
                     /locus_tag="PLJ75_03480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21372..21374),aa:Gly,seq:ccc)
     gene            complement(21542..21973)
                     /locus_tag="PLJ75_03485"
     CDS             complement(21542..21973)
                     /locus_tag="PLJ75_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD04310.1"
                     /translation="MANATLTGVFLSTQRHPDFNRLLKAETLKARYEHSKINRLDIDR
                     TVQLLMYIMNEEKAFADEELSLSQLADELDIRQQQLSRILNEKLNKNFSGFVNEFRIE
                     EAKKMLLEEPKRSILSIGISVGFNSATSFNLAFSRYEGCSH"
     gene            22696..24504
                     /locus_tag="PLJ75_03490"
     CDS             22696..24504
                     /locus_tag="PLJ75_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPD04311.1"
                     /translation="MASLDAVVKQVVSKYGNDKTRLMDIISDVQNELGCVSDDAVVQI
                     ARALNISTVDVDGVITFYHFFTKTPRGKYTIYLNNSAVAIMKGRAAVAKAFEEAAGCT
                     FGKTTSDGLIGLYDTSCIGMNDQEPAAIINGVVFTHLSKDKVQDIVAGMKAGKKINDL
                     VKEYGDGANQSELVRSMVKNNIMRKGPVLFAPYEKGAAIKVALAKKPEDVIEEVKIAN
                     LRGRGGAGFPTGMKWEFCRKAQGAKHYVVCNADEGEPGTFKDRVILTELPHMLFEGMA
                     IAGYAVGASEGVLYLRAEYLYLKKYLEKILDDLKAKNILGKNIAGKGFDFDIRIQMGA
                     GAYICGEESALIESAEGKRGEPRNRPPFPVEKGYLGYPTTVNNVETLCCTTRIMLEGG
                     EWFAKMGTAQSKGTKLLSVSGDCDNPGVYEVEFGTTISTLLELVGGRTAKAVQVGGPS
                     GTCIGKKDFGRRICFDDLATGGSIIIIGPNRDLLEIVHNFMDFFVEESCGWCVPCRAG
                     NVILKQKLEKIISGKASAKDIQELEEWCRIVKATSRCGLGQTSPNPIYTTIQNFREIY
                     EAKVKKDTDYVSTFDLLAAVEESCKTAGRIPKVEEH"
     gene            24506..25201
                     /locus_tag="PLJ75_03495"
     CDS             24506..25201
                     /locus_tag="PLJ75_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPD04312.1"
                     /translation="MSTINFTIDGKSCKAKPGQTIVEASKDNGIYIPTLCHFEGLKPA
                     GTCRICTVKVGGRNMAACTTPVTEGMVVENKTSELEDYRKAIVEMLFVEGNHMCPTCE
                     KSGNCELQALGYRYKMMAPRFPFLFPQRTLDASLPKIIIERNRCVQCLRCVRGITTSD
                     GKRIFGMVNRGGHVEITVDKELAANITDDMAKKAMDLCPVGAILKKEVGFAIPIGKRK
                     FDAKPIGSEIEKM"
     gene            25236..25775
                     /locus_tag="PLJ75_03500"
     CDS             25236..25775
                     /locus_tag="PLJ75_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HPD04313.1"
                     /translation="MSKPVVATASLAGCFGCHMSLLDIDDRILKLIELVEFNKSPIDD
                     IKTFTKQCDIGLIEGGCCNSENVENLKLFRKNCKILISVGECAIMGGLPAMRNGISVK
                     ECLEEAYLKCPTVDDNIIPNDDELPMILDKVYPCHEIVKIDYFLPGCPPRADLIWEAL
                     VALLTGKELDLPYEVVKFD"
     gene            25799..27247
                     /locus_tag="PLJ75_03505"
     CDS             25799..27247
                     /locus_tag="PLJ75_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HPD04314.1"
                     /translation="MARKITIEPVTRVEGHGKVTIQLDPQNNVLESRFHIVEFRGFER
                     FVQGRPYWEAPVLVQRLCGICPVSHHLAAAKAMDVIVGAGPDGLTPTAEKMRRLMHYG
                     QMFQSHALHFFHLVSPDLLFGVDADPAIRNVIGVALKFPDLAVQGVMMRKFGQEIIRA
                     TAGKKIHGTGAIPGGINKNLSIQERDEFLKGQDPMNITKMIEWAKAALDIFKDYHKKN
                     KDFIDSFATFPSSHLSLVRKDGALDLYHGVLRAVDSDGKKILNDVDYQDYLKYVGEEV
                     KSWSYMKFPYLKSLGKKDGWYRVGPLARLNTADFIPTPLAQKEFEEFKAYTKGKPNNM
                     SMHMHWARLIELLHSAEMIEQLLKDSDLQGDKLVAKGKKVKEGVGLIEAPRGTLFHHY
                     RINDNDQIEMANLIVSTTNNNEPMNRAVNWVAMNTINGKKEITEGMLNAVEVAIRAYD
                     PCLSCATHAIGNMPLEVSLVDADGNILDQKRK"
     gene            27250..27720
                     /locus_tag="PLJ75_03510"
     CDS             27250..27720
                     /locus_tag="PLJ75_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPD04315.1"
                     /translation="MPDVKCLIYGYGNPGRQDDGLGIELATMVENNPQLKHHVDVDYN
                     YQLNIEDALTISEYDKVIFIDAAFNIDKPFVFYTIQPADTITFTTHYLSAESIVALCN
                     DLYKKQPESYMLAIQGYNWELGLPMSEQANSNLQLAYNFISEVILNRFSCCITG"
     gene            27754..28131
                     /locus_tag="PLJ75_03515"
     CDS             27754..28131
                     /locus_tag="PLJ75_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04316.1"
                     /translation="MASKKLILVIDDDPDILDSIKAILTANAFDVVTAMSGKEGIEAV
                     SKTKPDLILCDMMMERIDAGTKVAQELKKDNPNLPIYLLSSIGTATATNIEIDKLGFN
                     GVFQKPVNPDNLVATIKNVLNIS"
     gene            complement(28218..29402)
                     /locus_tag="PLJ75_03520"
     CDS             complement(28218..29402)
                     /locus_tag="PLJ75_03520"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPD04317.1"
                     /translation="MAIKYLDQINVNNKKVMIRADFNVPYDKNMNITDDTRITATLPT
                     IEYCLKQNAIVILVSHLGRPKGKIVPEMSLKPVAKRLSELLKKEVLFIDKPITQGIQE
                     EINKLKPGDVVLLENIRFYPEEEKNDESFGKLLGSLTDVYVNDAFATAHRGHASNEAI
                     TRYVKECVAGFLMKEELEYFKKALVHPEKPVTAIIGGIKISTKIDVLKNIIPKVNNLL
                     IGGGMSYTFLKALGYNVGKSVVEDDHLATAKEIMELAKKYNVNLMLPEDIIVANKFDS
                     PEGKEVVPINNIPDNKEGVDIGPITRKKFSDVIKQSKTIIWNGPLGAFETKDFADGTN
                     EIAKVVAESACTSVIGGGDTGAAINASGYANKMTYISTAGGAFLELMEGKVLPAVKAL
                     DK"
     gene            complement(29482..30471)
                     /gene="tatC"
                     /locus_tag="PLJ75_03525"
     CDS             complement(29482..30471)
                     /gene="tatC"
                     /locus_tag="PLJ75_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPD04318.1"
                     /translation="MAREQKPKNDSTKNNSGKKRKNSQKKETKVTRKKKSLSTNSEDL
                     SNNQQKQEKKNLIDELIKEADNDQTSKSEIEENEVDPAALSRGDKPMNIVDHLDEFRS
                     RLLIVLITILGLTIIGFYFSDILLDAINRPFIDSGYKLNIFNLVEGFTLRLKASFVSG
                     LLIGLPLLVYQLWQYIKPAVAIENRKFAKYSSVAAIFLFYGGLSMTYFYLLPLSIKVL
                     LNFTPPDMDNMINASKYFNFAVLFSIAIGITFEMPIIIMILTRIGIVSPSFLISKRKY
                     AIVLIWIFAAVITPTTDPLTLSLVALPLMLLYELSIIISKFMVIRKKRKELSQ"
     gene            30779..33796
                     /locus_tag="PLJ75_03530"
     CDS             30779..33796
                     /locus_tag="PLJ75_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04319.1"
                     /translation="MFNDYTHLDAVVIPIIICDNKGKVKYVNNAFRTTKYSHSSDNEL
                     LNQQSVFTIFTLNENQKSEFKKVINNELNNITIILDTFQLLISKIKRNRTLVQIFKTH
                     KDFNTALHMYNANVDSFKNTFAPFFSAFDLLDDLIHITDKDGCIIYANKALNKLYGDI
                     KGQYCYSVFSKYDNYCPDCKMSLVINGSTIRYEAKLEKNGQYYDIINSPFIDFDGNIY
                     KISIMRNLEEIIDKQNTLLLFQNAINRSRNLVSFFKENGEISYANDTFIQYFGEQYKK
                     DINFLKLINCTNDELFYDVVDKEISLTLNNNLIFLKIFIYPIHSSDNEIIFVFIASDI
                     TNLKKLEKQLAVERNYFKSIIDNTIDGFFVINKNHNLIDCNPAFLKMFDLSYDNFKNI
                     RMEDLFDSQYKKDFDKKINYLNKTGISQITEVSHTKNGFKNYIISLNKLTSSEIDDAQ
                     YYGFVKDITDITRLHKIIENERNYNRRIIETVQLGFVVINEFGEIIDYNNAFLDLIGC
                     NGKNVKGEIYKSFIIPVSRDIMENFNVVSSNNPIEFEAIIQSSDNNNKNVIISMSKLY
                     DILGNYLGNFAFIRDITHQKKIENEILYQTNKVIELVNSLTQFTANVVRIDTIDNLLF
                     AISSFIQQLINPDYTEIFINNNTIFVSHKFNNGVHTVNNTEISQKTSFVVQKLTKNKT
                     ILYISNPLIELNDEDQLHFKNILEFQKIILIPIFIGDELKVILLSAFKTNEEKIDNVI
                     LTILTGVTNLLAITLEKIISVAEQNKMREALDRYDKLVAIGRIIAGVAHEINNPLSIM
                     QLDIAELNQKLQGDEYDNEIRDIIKSLFEEIQRISNIVKQLKDYSKPATLNDDIINLN
                     EIFRTYPLKILSKNIQKKGIPIVINVPKQKIFIKMPLTRLIQVIMNLLTNAEDAVIEK
                     GEGSIIITISQIQKDDICYACLSIQDTGIGIDEENMPLIFEPFFTTKPKEGTGLGLSI
                     SYSIIKSYNGEIEINSKKNMGTTVSVLLPAYKK"
     gene            complement(34000..35454)
                     /gene="ahcY"
                     /locus_tag="PLJ75_03535"
     CDS             complement(34000..35454)
                     /gene="ahcY"
                     /locus_tag="PLJ75_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPD04320.1"
                     /translation="MTQPSGKGMLNIAEYDHKLPFKVKDITMAELGRKELQLAENEMP
                     GLMALRKKYGNQKPLEGLRISGSLHMTIQTAMLIETLYHLGADIRWASCNIFSTQDHA
                     AAAIAQAGTAAVFAWKGETLEDYWWCTEQALIWPDGSGPDCIVDDGGDATLFVHLGVK
                     AENDSSIINKNYDSKDMQCLIDRLQLSVSKNPNRWTTIASKIRGVSEETTTGVHRLYQ
                     LEKSGELLFPAINVNDSVTKSKFDNLYGCRESLADGIKRATDIMVAGKVVVVCGYGDV
                     GKGCAQSMRGFGARVIVTEIDPICALQAAMEGYEVKTLEDVVESGDIFVTATGCCDVI
                     RGEHMERMKDEAIVCNIGHFDSEIEMSYLENNDKCKRLNIKPQVDKWMLEKGHSIIVL
                     SEGRLVNLGNANGHPSFVMSNSFTNQTLAQIELATNQALEKKVYTLPKKLDEEVARLH
                     LGRLGVKLTRLNQKQASYLGVNIDGPYKPDHYRY"
     gene            35568..36104
                     /locus_tag="PLJ75_03540"
     CDS             35568..36104
                     /locus_tag="PLJ75_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPD04321.1"
                     /translation="MKCKNIFYVLSLILILSLSSCTPSQWHYRNIPLKRKQIINTATK
                     YIGIPYRHGGSNPSGFDCSGYVYYVYTKNGIPIPRSTTMQFKKGKKIPFRVAQPGDLV
                     FFSIQSSGVSHVGIFLGNDTFIHAPSSGKAIRYDLISNPYWKKRFVATVTYLNNYSQL
                     NEMKTIGSSYSEERFEWK"
     gene            36190..36669
                     /gene="coaD"
                     /locus_tag="PLJ75_03545"
     CDS             36190..36669
                     /gene="coaD"
                     /locus_tag="PLJ75_03545"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPD04322.1"
                     /translation="MQIGIYPGSFDPLTCGHLDIIKRATQLFDRLVVAVARNSEKFPL
                     FSVEERLAMLSECCRDISKVELTSFDGLLAEYCKKNNINFIVRGLRAIVDFEYEYAIA
                     LMNKEIAPTVETIFLMSKSEFSFVSSKMVKEVAGYGGDISRLVPQFVNKKLKEKFQS"
     gene            36739..38274
                     /gene="rny"
                     /locus_tag="PLJ75_03550"
     CDS             36739..38274
                     /gene="rny"
                     /locus_tag="PLJ75_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPD04323.1"
                     /translation="MSVLTILLIVALPLAAVSGYLFRAYIGKIKLTTAEAQSRKIIQD
                     AVKEAESKRKELLLEAKDQLLKERNQFEKEMRERRIELQNIERKILQKEEMIDNKIQQ
                     LEKQEKAVQLKEKENIAKEQELNKHLEKHKQELERISGLTREEAKQLLLKNLENEVRF
                     ESIKLINKVEEEARRSADKKAKEIVLAALQRSASDYTQESTITTVSLPSDDMKGRIIG
                     REGRNIRTLENLTGVDMIIDDTPEVVVISGFDPIRREIARLSLERLIENGRIHPARIE
                     EVVEKVKNELEENMLDEGEHAAFELGIPGLSRDALYYIGKLKYRSSYGQNVLSHSKEV
                     ANLAAIMAGELKLDVAIAKRAGLLHDIGKGSIVEGEGAHAIVGSELAKKFGENDIVVN
                     IIASHHNDKEPESFEAILVQVADAISASRPGARRESLDTYLKRLENLENIAYGFKGVE
                     KCYAIQAGREIRVMVSNEQVTDEEATILARDIASKIESELKYPGIVRVTLIRETRIVD
                     YAR"
     gene            38336..39139
                     /locus_tag="PLJ75_03555"
     CDS             38336..39139
                     /locus_tag="PLJ75_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPD04324.1"
                     /translation="MESLKVLFIGDIVGKPGRAIIKNYLSDICQEYSIGLVIANCENA
                     AGGMSITPEIANELFSYGIHVLTTGNHIFNNRNSIQLLETNTHFLRPANFPDGVPGTG
                     FTTLTINNITIAVINLIGRIGMEPVDCPFIKFNTIYETIQSTSDLIFIDFHAEATSEK
                     KAFGWFVDGKATAVCGTHTHVQTADECILPQGTAYITDVGMTGPFDSVIGMDKDSSIH
                     NFIYHTRIKFKVANDDPKLNAVVITCNNVSKAVNIQRIIKSYEISTTNP"
     gene            39228..41150
                     /gene="dxs"
                     /locus_tag="PLJ75_03560"
     CDS             39228..41150
                     /gene="dxs"
                     /locus_tag="PLJ75_03560"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPD04325.1"
                     /translation="MLDDINGPEDLKKLSIGELSSLSEEIRSRILEVVSHNGGHLASS
                     LGVVELTIALHYVFNSPTDRIIWDVGHQCYAHKIITGRNKLFHTLRMFHGISGFPKTS
                     ESVHDHYDTGHSSTSLSLALGEAIARDINSGNYQIVAVIGDGSLTGGMSFEALNHIGQ
                     SKKDIIIILNDNEHSISRNVGALSEYLMRLITGGVYNKLRRKSYEFIHRIPKIGPMLF
                     NLFDRIEARIKGIFVPGSFFEDLGIRYFGPIDGHNIPTLIDLFNRIAKLNKGPKIIHV
                     YTKKGKGYVHAEKNPSKFHGIGPFDIKTGKPLKKSSISYSDIAGKALVDVARRDKKIF
                     AITAAMKLGTGLSEFEDHFPQRFIDVGICEQHAITLASSLAKNGFKPFVSIYSTFMQR
                     AVDQIIHDVALMNLPVKLLIDRAGIVGDDGETHHGLFDIGIIRMIPNFVLLSPTNGNE
                     LRDMIHYASIYNNGPIAIRYPRGVDDKYVHSANSELIKLPLLKAYYKSIQPMIALFAV
                     GDMIKLATEVHMKLNEHDIKSSVYGILSLKPLPINQIEIILQNIPYYTVFENCYTSGG
                     ISEYLNSQINCSLRYKNIYNVAFPDNFILHGRVSELFAHYSMDSDSITNKIISLIHTE
                     KVSHVKKSLRSISSSK"
     gene            41110..41919
                     /locus_tag="PLJ75_03565"
     CDS             41110..41919
                     /locus_tag="PLJ75_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HPD04326.1"
                     /translation="MSKNRLDQYLLQNKICVSREKARNLILAGCVSVNGQIVLKPSFI
                     INENDNIILLQASQRFVSRGGEKLQRALDIFDINISNRIVLDLGSSTGGFVDCLLKNG
                     ARKVYAVDVGYGQLDYSLRMNPNVIVMERHNARYVIRDDFSDNIDFITADLSFISIAK
                     VMKNIVTIFDSTIDSVLLIKPQFEAEYYQHKKGVVKKPEYHYDIVYKVIHELTLLDIQ
                     IKGLTYSPIKGPKGNIEFLLLCTIHPDTDTNIKDIQLLIKNCVDEAHKILD"
     gene            42066..>43324
                     /locus_tag="PLJ75_03570"
     CDS             42066..>43324
                     /locus_tag="PLJ75_03570"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HPD04327.1"
                     /translation="MKHKTVVRKKQPQKIVSVENDWKEAIKATLFETVLRFGIAAYCE
                     MVEEEITALCGERYKHLFKREYTRWGKKNTSVVLGGRKIALQHSRIRDIQNNGEKAIA
                     IVEQYKGKEVLSQRQMEQMIIGVSSRKYGRSLELGKGAVESHAYSKSTVSRNFIAKTQ
                     EKVEAWRHAAITAEYPFLMIDGIVYATTTVLVALGIDRDGTKKVLGAWEGSSENSRVC
                     IDLLQHLVERGFDVTKCFLVIIDGSKALRKAIHDVFGDSILVQRCQVHKERNVIDYMP
                     KGQREAVKRALSEAYSADTYDRAKRLLGNIHQWLAKDYPQAARSLEEGLEETLTLHKL
                     GAHKKLRKSLGTTNPIESLNSGIRTVTGRVKRWRNATMVMRWVWSGIIESERNFRKIN
                     GHTYIDALLLNIAMFGKKVIEENNKIA"
BASE COUNT        14964 a         6424 c         8090 g        13846 t
ORIGIN      
        1 tacacctttt ctttttataa ggaaaatggc atacattgct gccaatacca ctggggacca
       61 cccccggaag tactccagca gaacgcttag tttctgatta taccatttta ttgttttagc
      121 atcaagctga ttagggtatc tttaaataaa gctacataaa gctttattta aagtattatg
      181 gccatacaat aatgttaaaa ttgcattttg aatgttgata atatcaaaaa aagttttatt
      241 gatcccatta aataaaattt gatttgctat aaataatggt tatattgtag ttgttacaac
      301 aatattttaa acagtaattt aaattatttg acaaattttt atagcaaacc aagagttgaa
      361 aaatcaattt aatagggagg cttaaacaat gccaaatctt ggttttgggg aattagtaat
      421 aatatttttt atagtcatca ttgtttttgg tgctgggaaa atacctaaaa ttgctaaaga
      481 tataggtagt ggtattaaag agttcaaaaa agcactaaat tcagatgatg atgtaaaaga
      541 tgaaaaaaaa tcatgataaa aaaatacacg ggatataacc atatatttat gggtgtatcc
      601 tgtgtagatt tttttttgta gtataaataa atatatagaa taatgtatat ttttgcacat
      661 tgatagagag tatatactgg atttcattag ttatgaatac atcaagctaa tttaaaaata
      721 gctactgtga aacaatctat gaaccaataa taccatatac aaatatggta atagaaagtg
      781 ataattttat aaaaataaat ttacaaaaga tcaatatcaa tagtagcatc aaaaaactta
      841 tcaattgtaa tgcagtacaa tgctccattg gcagcttgaa ttggtaaata gttcaaaaag
      901 tagtattaca gtagtattaa tagtgatata attttattgt catttaaaat tcaattttta
      961 ccatcataga acatgcatac aatatgaaaa aatatattaa aaacttttct cttgaggaag
     1021 ttgaacagat atttgcgtca ataaatgaaa agagctatcg agcaaaacag ctttttaact
     1081 ggttgtatga gcgtaatgtt gataatttta acacaatgac taacttctca aaaaaattac
     1141 gtcaatattt aaccgataat tatgaaattt ctgcattaca attaatagat cgtaaaaaat
     1201 caaattgtga tggtactgaa aaatttttat ttgcaacaca tgacaatcat ttcatagaat
     1261 cggtatttat ccgcaatgat ggtaccgacg aggggagatt gaccatttgc gtttcaagtc
     1321 aaatagggtg tcctatggga tgtacctttt gtgaaacagc aaaaattgga tttaagcgca
     1381 atctggaatg tgcagaaatt cttgatcaga ttaataaagt acgacagcta accggtttga
     1441 aaaataacaa tatcgttttt atgggaatgg gtgagccttt tttaaactat gataatgtca
     1501 tgaaagcagc tgctattatg aactatacat ttggatttca tatatcagta aggaaaatta
     1561 ctatttcaac ctgtggtatt tatgaaggta tacagcgctt tattgatgaa aaaatgccgt
     1621 acaatttagc aatatcattg aatgataccg atatggttaa gcgttcaaga tatatgccgg
     1681 ttgaaaaaaa atatccaatg caaaatatta ttagattact ggatgtaaaa tttcctgttt
     1741 cacgtaacag agtaaccatt gaatacgtca tgcgcaaaga taatatatct gaagaagatg
     1801 cgcaacgttt aaaaagtatg ttcaaataca acagaataaa gttaaatctt atcccattaa
     1861 ataaaggaaa acataacatg gaaattccag ttgaagaaga aataaataaa tttataaaaa
     1921 gacttgaaat tatgaatata cccatatcta taaggaaaag tgcaggcagt gatatagatg
     1981 gtgcatgtgg tcaattgtct ggcagacatt atttataaaa aatatttgta cgatagtagg
     2041 tccggataat gataactgat ataattgcat ggattcgtga gcataaggta tttacttttg
     2101 tcggtattcc actcatatgt tgttttctca ttattctgta tacatcggtt gtttatatag
     2161 aatggctgca tgataaacaa aacgcactgg ataaactctt taaatataaa cagcttattg
     2221 accgaactga agagctaaaa ggaggttatt cgtataccta tagcgaaata gatgtttcgg
     2281 caaaagtagt agatattcct acccgcattt atgatagaaa taacgaaatt attggtgagt
     2341 tttttgaaca aaaacgggag atagttcctt attcatactt tccaaagtgg cttattaaag
     2401 ctgtcattgc aagcgaggac agaaattttt ataatcacaa aggtgtaaat tacaaaggta
     2461 ttgcacgtgc atttattact aacatcattc attttcgggt tgtacaggga ggcagcacca
     2521 ttacccagca acttgcaaaa gtattgttta ctgatatgga acgaagtata aaaagaaaga
     2581 tatatgagat attctgtgca tttgaaattg aaaaacacta tgataagcag gacatcttat
     2641 ccatgtatct taattatatt tactttggta atggggccta cggtgttgaa gcagcatcaa
     2701 agatgttttt tggaaaatcg atagttgatt gtaatgaaac agaatgtgca atgattgttg
     2761 caaccatttc taacccgttg atttactcac cacttaataa tcttgattta tcacttaaaa
     2821 agaccaaacg gatcatgcaa tccatgatag atgctggatt tataagtagc gaccaggctc
     2881 agttacaata tagaaatttt atcaagcaat gggaggtcaa gttttcaaag gatactatcg
     2941 ccgaatcttc tttaatagga agtttcatct ttagcctgta tcgtatcaat agggcgccgt
     3001 tttttaatga atatatacgg cgtatactgg ttaataaatt ttcagatgaa gttgttaaaa
     3061 agggtggttt aacagtatat acaactatcg atgctaaaaa acaggatatt gccattacag
     3121 cattgcgaaa aggtattcaa cttcaacgtg aatatcatga gaaaatagct aaagcattaa
     3181 aaaatcctga tagagcattg ctggaaatgg agaaagccaa taatattgag ggagcatttg
     3241 tttcgttgaa tccttttacg ggtgaaatta tatcatatgc tggtggctac aatttttcat
     3301 atcagaacca gttggatcat gtacagcaga tacgacggca accaggttca tcatttaaac
     3361 ccattattta cgttgcggca atacaggata aagtaatcac tccttcatct gtttttgatg
     3421 atattccaat gaccttcaag ggtgggtatg ctccaaaaaa ttatgatggt ttttattccg
     3481 ggaagataat aattcgggag gcattaaaaa agtcagtcaa tattgttgca gtgcaggtac
     3541 ttgaaaaaga aggatattca cgggttatgt ctattattca aaaagggttg aatcttacca
     3601 atagtgaatt ggattcacga ttttataaaa cattatcatt agcattaggc acctatgaac
     3661 tttctccgct ggaaaatgca gtgttacatt ctgttattgt taatggtggt gattttgtag
     3721 taccctatgg gatacgatat gttaacgatt ataatggtaa tattgtatgg aataatgaag
     3781 aagaagtgtt gcttcaaata caggaaaaac ggaaagaatt tggtaagatt attgaaccgt
     3841 ctgcatgtgc catcgttgtt tcaatgttga aaggagtttt tgaggaaggt ggtactgcct
     3901 catggatact aaaaaataga agtacaccgg ttgaaattgc aggtaaaaca ggtacaagca
     3961 gtgacttcgt tgatgcatgg tttatagggt acactcccga ttctgtgagt gcagtatgga
     4021 ttggtaataa agcaggtgcc atatcattag gacgtggaag aagcggtggt gttttatctg
     4081 caccagtttg ggctgattat atctttgcgg cttataaaga tgcagaatat aaatcctttt
     4141 caattcctgc tgatgatatt acaaaacaaa ctatatgtct tgatactgga ttagttcctc
     4201 ggggagaagg gtggtgccct cgcattgcaa aggatgaatt attttatgcg ggcactgagc
     4261 caggtgaata ctgtactatg cacacagtgc ctgtattaaa gtgatggtga tactatgaca
     4321 aaatcaatcc atttatcagt aactatcgcc ataattgtcg tatcattgtt gtcggtttca
     4381 tttcaattgc cagtacaaaa cggtactata cattcaacat ttggtgaacc tcgatctgac
     4441 cattttcata atggtgttga tatatcctca attgataaaa aagtttatcc agttaaagat
     4501 ggaaaattga tttttttgtg ggataaatca ttgtttcccc ttgagcagta tcccggtgtc
     4561 ggcaattatt gcatacttga gcataataag caagaaaaat ctttgtatgt tcatttagat
     4621 aatggtatta gtatgagtga taattatacg gcaggtgata gttttgcaat aatgggcaat
     4681 actggcagct catatggtac acatcttcat tttgggatta taaaaaatgg aaggatttcg
     4741 attaatcctt ttgttgtatt accacatgtt gacgatgtca aaacacctgt cattgaagcc
     4801 atatacatta aagttgaaga taaatatacc aatatccatg agggcagcac tatacggcta
     4861 acaaagcatc atcctattgc aatccagtgt tttgatgctg ttaaaaaaaa tgagcgatgt
     4921 ggaatttata aattaaaagt gatagttaat aatgatatag tggctgatta taaatttgat
     4981 tctattgttt taaatgataa aaataagttg acaattgatg gctttgcatt tgagaattta
     5041 tatgatgcaa aaggtaatta tttagtgtgg tctattaatt ataaacaggg tatcaataca
     5101 ataaatgtta ttgcagaaga ctttgctggc aatcaaacct caaaatcaat tagctttatg
     5161 gtggtgcttg attttaaata ataaagagct ttcatagcaa ggaggggtat ctttgtgtcg
     5221 aacatgattc tattttttcg aaggaaaaaa aatatcaagc aagttgatat ggctaaagct
     5281 ttaaatgttt caccatcata tttatgtaaa atagaacgtg gggttcagga acctacagag
     5341 gaatttaaga ggatgtgtgc cagatattta aatataagtg tatcaaaatt atttcctgaa
     5401 cgtgtaaaaa attctaaaga tgtaataaat gatattgtta ctgccaaaaa taaaatatgg
     5461 aatattcgca tggcaaaagg tattaaacaa tatgaattag caaaattatt gcattgttct
     5521 ccatcatatc tatcaaaggt tgaaaagggg ttgcaagaac ctaccgaaga atttaaaaaa
     5581 gtatgtgcaa aagttttaaa agtaaaagaa atagagttat ttacttaacg tatcaggttt
     5641 aatgaatacc tatgactctc tcgatacact tacaatttgt tatcatactt gtgtgtttgt
     5701 ttttttcagc atttttttcc ggatcagaga cagtattgtt ttcttttaaa aagagcgagc
     5761 ttatacggtt ttcattttca gaaaataaat tagaaaatca aatcgcaaaa tttttatcgt
     5821 atccgcataa tattttaata acaatcctta ctggaaattt atttgtaaat attttaatat
     5881 cttccctgtc aacttcattt ttgctaactg tatggccaca gtatggacat attattgcaa
     5941 ttctttttgt tactcctgtt atcattttat ttgttgaaat actcccaaaa attgtatcgc
     6001 tctatagcta taaaaccatt gtttattata ttatgcctgc gatattattt ttccataagg
     6061 tgttactacc tgtcagatat gttttacttt tcatatcaaa tacttttatt aatgtattca
     6121 aattaagaat agattcatcc tttgatattt cagaagatga tttatctatg gctatttatt
     6181 atggagagaa gtttggattt ttatcacaag atgagaagca atttatacat aatctattac
     6241 gttttacaaa aaaaactgcc gaaaatgcta tgatacatag aagcaaaaca attttcatac
     6301 cctataatgc aacaatttct gaagttataa gcatagttaa agaaacaggt attgtgagag
     6361 cccctgtata caagaataat cttgatacaa tcgtgggatt aattgattca agagatattg
     6421 tttcatatgc aactggtttc aaaaaaggta catcaataaa aaagatcata tatccaattt
     6481 atcattatcc tgcttcaaga gcgctgggtg aacttttaga agaatttttg cgcaataaga
     6541 ttcagatagc agttatagtt gatgagtatg gtggtacgct ggggattgtt accttgaaaa
     6601 gaattatagc cgaattattt gggcgtgggt atacattatg ggaagatacc cctaaacctg
     6661 aaataaaaaa agaaaagggt gctgtgataa taccgggtga tatgcaaatt gatgaattta
     6721 atatgatgtt taatgtttca atagaatcat cagaaagcga gacgattggt ggttatatta
     6781 ttgaaaagtt aggatactta cctaaacgta gagcatccat aatgattgat gattatatag
     6841 tgacagttcg ttatgttgca aaaaaccgta tacaatccat tgaagtgaaa cgagcagaat
     6901 catgatgaat atctatattg gtacacaaat tataatattg atatttatac ttttttctgc
     6961 ttttttttct gctactgaaa cagcattggt ttctgcaaat gttttataca ttcaaaatct
     7021 tatcaacaaa ggcaataaaa aagcaaagga tattctttac ttaatcgaaa atcctgataa
     7081 agcattaacc atgcttctga taggtaataa tattgccaat attggagcta cagcgtttat
     7141 cacttttttt gcaagcagag ctcttcttat acatgattca aaattattta tagtaactat
     7201 cgtccaaaca atattctttt taatcttttg cgaacttttc ccaaagattt attcacgttc
     7261 aaagtcagaa aagattttat ttttcaacta ttggttaatt aagattttta acattttgtt
     7321 tatgccatta atatcgctca ttatttttat tactgatttt gtaaaaaaaa ttattaacat
     7381 aaacgacacc caactgctgc ctataagttc gcgagatgaa attgatacat tgtttcgagc
     7441 tgttgagaaa gaaggcatta ttgattcaca acattatata tattttaatg agatactttc
     7501 attcaaagac gtgcgcgcca atgaggttat gactcctttg attgatatta tctcaataga
     7561 gaagaatcaa tcgataaaac tatgtattac gttaattgag aaaacacgat tttcacgaat
     7621 tcctgttttt gaagaacgtg ttgataacat tgtaggctac atctactatc aggatatatt
     7681 aagaaaccca ttaataaaaa atcttcagca gataatgcat ccaccacatt ttgtcccttc
     7741 aacaaaaaaa atatatgact taataaagga gatggaagca aataacgttg caatggtttt
     7801 tgtggtaaat gaatatggcg ctgtagaagg attgcttact gatgaggata tagcagaaga
     7861 gatagttggt gaaatacaaa caagggatca tcctcaggat gaatcaatta aagagatagc
     7921 tccatggaaa tacagtattg ccggtaattt agatattgaa ttttttcagc gcagatttgg
     7981 tataaaaata gaaaaacatg attttgaaac ggtagctgga tttttattat atcaaatggg
     8041 atatatacca aagccaggag aaaaaatccg ctacatggat ataacattcc agattgaaag
     8101 agcaactgat cgtgcaattg aaagggttat tgtaactata ccaaaacgca aaactactat
     8161 tgcacaagca taggacccaa atcgtcttta cctgacagaa tgtgtacatg cagatgccat
     8221 attacctgac ctgcagcaga tccattatta atgataacgc gatacccttt atccaatttt
     8281 tcctgctcag ctattttttt tattgcaagg aaaatatcat tgatgatata gctgtttttt
     8341 ggggtaagtt catcaatgtt ttttatatgt tctttatgta caacaagtac gtgaaaggga
     8401 gcttgcgggc ttatatccct gaatgcataa accttatcat tttcaaatac tttgtttgaa
     8461 ggtgttttcc ctgtaataat atcgcagaat aagcatccca taaatatttc ctccattaaa
     8521 aatttatatt tactatagac catgtgttga taaaatagat tgtaaactaa aaaaaattca
     8581 agaaaaattt tagaaaatga gaacaattaa cgaaatacag tatcttgttt atcctcataa
     8641 aaatattaat gatgaaattg ttaaaagatt ttatagtaca gcaatacctc ataaaatacc
     8701 gtataaaaac gaagatgatc ttgtattaat tgtaaatgag ttgaaagata gttgtattga
     8761 ttcaaaggat gttattatca taaagccgtt tttaggcaga ttttatcagg tttgtcctgg
     8821 gtcgccaaat atgatctgtt gcaggtacag ggttattaat acctgcttta attgtttata
     8881 taattgcact tattgctata tgcatatcta tttgaacagt tatggtatta tacagtttaa
     8941 taatactgat cgactttttg ctgaattgaa taatttcgta acaacttgtt catcatcaat
     9001 ggtctataga attggtaccg gggaatttac cgattcactt atgatggatg aaataaccaa
     9061 tttgggaagg caattgatac aatttttttc tgttcatgat actatctttt ttgaattaaa
     9121 gacaaaatca tccagcgttg atcatttact tgatgttcaa acaaaaggca atgcagtatt
     9181 agcatggagc ttaaatacca gtgctaatat agcactgtat gagcatggta cagcatcgtt
     9241 gactgaaagg attaatgcag ctgttaaggc tattgatgca ggttattata ttgctttcca
     9301 ttttgatccc attattatgt atgataattg ggaagaggat tattacaata taatagcatt
     9361 attgaaacag tatattaatc cacagcgggt tgtatggatt tcgttaggag gttttagata
     9421 tcacttacat tttagagaaa ttttacggaa caaatttaat catgaaatga tgacaacagg
     9481 tgaaatgttt ccgggaaggg atggtaaata tcgttatttt aaacctatac gagtgaaaat
     9541 gtatcatttt atgtataagc atttacagga actatttcat aagacatata tatatctatg
     9601 tatggaaact gatgatgtat ggcatcagat aacaggaaaa tattttaatt cagccgagga
     9661 attggaatca gatatggcta atcatttata taaacacttt gtgcaaaaaa aaaccatttt
     9721 agattgaccg tgtgctatat tgcatattta ttgtaattaa taaagattgt cattgtattt
     9781 tatgtaatta ttaaatggga gtacatacat tggaaataat tgagacaaaa gatctgttac
     9841 gggaattgtc ccaaataaag aatgatattg gtgaacttta tactacactg aatatagatg
     9901 ataaaaaaaa gcaattaagc ctgctaatag aagaaacgtt taaagaaaat ttttggcagg
     9961 atcaagcgca ctgcacacag attcaacagc agataactca atttaaaaga actatcgaac
    10021 cattggttgc cttaaaacaa gaagtagaag attcaattgt tttgctggaa atggccattg
    10081 atgagaaaga taatgacgta atgaaggata ttggcagcaa gattgagcaa tttcgcaacc
    10141 gatttgagca actggaaaca tatgaattgt tatcaggtgc agatgatgat aaaaatgcca
    10201 ttatgacgat acatccgggt gcaggtggga ccgaatctca ggattgggca agtatgttga
    10261 tgcgcatgta catgcgatgg gctgaagata atgattttga ggttgaggtg ctggattata
    10321 tgcctgatat tgaagcaggt attaaagaag caaccttgct tattaaaggc cagtatgctt
    10381 atggtttttt aaaaggggaa agcggtatcc acaggttggt tcgtatttcc ccatttgata
    10441 gtaatcagag gcgacatacg tcatttgcat cggtagatgt tatccctgag gcaccagaca
    10501 atattgatat tgatataaaa gaatctgatt tgcggataga tacttaccga gcatcaggat
    10561 ctggcggtca acatgttaac cgaactgatt cagcagtgcg tattactcat attccaaccg
    10621 gaattgttgc tcaatgtcag aatgaacgtt cacaacataa gaataaagca tatgcaatga
    10681 aaattttaaa atcaagattg tacgaacgga aaagacagga attagagaaa gaaaaaatgg
    10741 aaaaatttgg tgaaaagaag gatatcgcat ggggaaatca aatacgttca tatgtttttc
    10801 atccatacac aatggttaag gatcatagaa caggggaaga aacgggttcg gttgaatatg
    10861 ttatggatgg agaaattaat aaatttatat atgcttattt aaaaagctta aaaaatatta
    10921 atcgtaaata aaaaagcaat tcttcacttt gcttatttgt attcaatgca ttattggata
    10981 tttaaaatat ctaagcttga ctattttttt attgcataaa ttataattag tcatgtactt
    11041 attataataa atacgaatat atactctata gtttttagtt attagttgtt ggaatatctt
    11101 ttataatttc attatgcatc aacaatagta cgaaagcatg agtgatatat attcgtaaaa
    11161 atgtaattat tcaattatat gataaattat aaaaaatata tactatatac actttcccta
    11221 ttgttactta tattatttat catcattaat gcaataaata ctatctttgc tgctggttca
    11281 ggaggaactt caggtgcaga ttttctggaa attggggttg ggagccgtcc tttaggaatg
    11341 ggcgaagctt ttactgcaga ggttaatgat ataaacgcaa tatattacaa tcctgcaggt
    11401 cttgctacat taaaatttcc tgtaatgtca ataatgcatc aggaattgat actggattca
    11461 cggtttgaaa atatatcatt agctttacct ctttacggtg gtttttttgg attttcaaca
    11521 tctgttttct gggttccccc ctttgacaaa attgatatca atggtaataa agtaggtact
    11581 gttacttttt ataatgttag cggcattctt tcgtatgggt actcactggg gtttatggaa
    11641 attggtggat cattcaagta tatttatcaa aagatagata ccttgcaatt acattctgtt
    11701 gcctttgatg taggtatttt aaaacgtatt accatgtatt ccccatttga tgctcctata
    11761 aaaaatttat catttggttt atctgttcaa aatataggaa ccaatgcaaa cgatgacagt
    11821 ctgccacggt taatacgagg cggggtttcc tataaaatgt cgcatttttt tgggattaat
    11881 gttgacttta tagaaaatgc cattaattca tcggatcttt atgatttcac ctccggattt
    11941 aatgaaagct ttagagtaaa taccggtatt gagttaaatt atcttgatat cttatttttc
    12001 agaacaggct ataggtttaa tgatgctgga acatatactt ttggtatggg ttttaactat
    12061 gcaattaaaa atgttgcatt tgtcatagat gcgtcatatc aggatgctgg agtatttgga
    12121 ccaatctact cgttcacagt aacatttaaa cttattccca gggtaattac aatagaggat
    12181 aagctaaaag ctgaggcaca ttatcaacgt ggtatcaaat actttataac ggatgatata
    12241 gatggtgccc ttgaagagtt taaaaaagcc agtgattatg atccatacca caaaaatgtt
    12301 gataaaaaaa taaaggatct ggaagaactt aaagagttga agaggcaaaa tgagttactt
    12361 gagaaagaac aaaagacagt acaatgaaat gcgtgaagtg acagtttata cagactattg
    12421 ccccaatgcc ctgggttcag tactgtatca atgcgggaac actcaggtta tatgtgctgt
    12481 atcatgctca aaccaggtgc cccagcatgc actggataaa ggtacgggat ggttaacagc
    12541 agagtataat ggttaacagc agagtataca ttgcttccct atgctactgt tccaagaaca
    12601 gaacgaaata ggataaaacc tgatggaaga tcggttgaga tacagcgtct gataggacgc
    12661 tgcttgcgaa cagtggttga tttaactgca ttacaaaata ttgcattggt tgttgactgt
    12721 gatgttctac aggctgatgg tggtacacgt actgcagcca ttaccggtgc atacattgct
    12781 ttacagatag ctatccagaa attgatacat cgcaatgtaa tagatgttaa ccctgtaatt
    12841 gccatggttg ctgcaatatc agctggatat ataggtgata gcttactgtt agatttagac
    12901 tatgcagagg attcacagtg tgacgtagac atgaatcttg ttatggacag caattaccag
    12961 attattgaaa ttcaaggtgc tggtgaaaaa cgttcatatg ctttccatga acttgattcc
    13021 atgataacta tcgccaaaaa gggtatagaa aaactcttcc agatacaaaa tgaaatactc
    13081 aaattgtgct gatattagcc gaatataaat atataattaa atatcctatt tcaaaataac
    13141 ctggatggta ttacaataat gcaaaaatta ataatgatta taatgtgtat tttatgcttt
    13201 gatgttgcat atgctcaata ttacgttcgc aatacaacgg taatagatac tcctactgcc
    13261 tatactattg cacgtggcac ctatcaatta tcatttttag gttatgacaa cggtggagtt
    13321 gaactaaaag catttcttgg actgcacaat aatttttatc ttggtgcttc atttgattgt
    13381 caaagtgcaa tagggaaaga tgatcctgat ccaaatgttc caggcgttgt aggcaagata
    13441 aagtttaccg atggatggga gatgttcccc atttcatttg ccattggcta tgattcattc
    13501 tacattggtg aagagggtaa aacatataat agcaaaaatg atcttgacca ggtaatttat
    13561 ggcccttatt ttgtaataac aaaacctata tatctttttg atgatgaaca gcatatacat
    13621 tttggtatgc gaatgccaac acagcccgat tatgttcccg aagatacctc atatttttta
    13681 tcgtttgata ttccactggg tgatatgttt gttttcaaag cagagatgga acgtgtttat
    13741 tataatttca gtaatcccga ggattggctt tataatgtgg gcatgcgata ttcatacatg
    13801 cagcgactgg gcattgaatt tgatgtactc ttccagaagc atgaaaatcc aaacaggatt
    13861 atacgtattg aatatattaa tgagttttaa ttatagcgac atcaatttaa atgacttatt
    13921 gaaaaaatga gaaaaaaaat tatattaata tatatgagta gtatagtagt tttctgttca
    13981 atgatttatg gttatgcttt ccttaccaat catagtggat atatatatgc taatgataaa
    14041 cggcattgta tcataacaat agttaataca ttaaatgaaa aaatacaatg caatgttgag
    14101 atagctctca cggatgaaga gcatgcaact ggattgatga atagaactgc attatgtgat
    14161 gattgcggta tgctgtttgt ttttgcagat gaaacttacc gaactttctg gatgaaagat
    14221 accgctattc ccttatcgat tgcttttatt gatgccaatg gtattatcaa tgatatgcat
    14281 gatatgaatc cattccagac ttttccagaa tactcatcaa aataccctgc tcgttatgca
    14341 ttggaagtca ataaaggttg gtttaaaaaa aatgccatat cagttggttc aaaggtaata
    14401 atgcatggat gcatcagtaa ataggattcc tatttcagaa aaagaaagag caatccttat
    14461 tggtgtgttg ctacctgaag ctgatgaatc agaaatatca cattcactgg atgaattaac
    14521 acaattggtg catacagcag gtgcagttgt agtaacgcgc tatattgtta agcgcatggc
    14581 ttttgatcca gcatacttga ttggaatggg taaacttact gaaataaaga accttattgc
    14641 agaacaatct ataaaacttg ttgtttttga tctaaataca atcaggccgg tacaaatacg
    14701 caatttagaa aattatttac actgcagagt tataggtaga actgaggtta ttttagacat
    14761 ttttgcaaga cgagcaagaa caacagaagc aaaaattcag gtagagcttg cacaacttaa
    14821 ctatatcctc ccgcgtctca agggtttggg tggagtttta tcccgtttag gtggcggaat
    14881 aggcaccaga ggacccggag aaaagatgtt ggaaactgac aggagacata tattacgaag
    14941 aataacaaca ctgaaaaaaa aattaattca catatcacat agaagagatc tacaaaggca
    15001 gcaacgttta cataatgaaa taattggtgc tgtagtaggt tatacaaatg caggtaaatc
    15061 cacattggta aatatattgg ctaaagatga tttgtttgta gaggatagac tatttgctac
    15121 ccttgatcca tatacaagaa gtgtttattt agataaccaa cacaatgtat tacttacaga
    15181 taccgttgga tttattaaca atctcccgtc acatcttatt gaatcattta aatcaactct
    15241 tgaagaaata aaatattctg attttattat tcatgttgtt ggattggata taaatattga
    15301 tagggcagta gcagttgttg cccatgaact gcaattgtta gattctaata ataaacccaa
    15361 tattttattt tttaataaat gcgatattgt ttcgcccgat gttatacatc atgtacggct
    15421 gttataccct gattcaatta ttggttcatg tttaactggt gaaggtatca tcaatttaaa
    15481 agaaagaatg ataaaaataa ttgaaaccac tcatcataaa aagcagcaat caatcataaa
    15541 caaaaacatt gaactgaaat atcaccattt aaattgatta attcatgtaa cagcagatgt
    15601 cgaatataat agtatggtat tttaatgaca aatttatata gttctgggta ggctttgcat
    15661 gtatgatttt aaacgatata cctttcctga aaaaaggaaa ataaataata gtatctatgg
    15721 atttttctct attctattta tccttgtaat ctgtattttt acattaattc ccggaaaatc
    15781 aaataacaaa ggaattagcg cagcgtctaa tcttaattca gtagaaaata agcttatcat
    15841 cgatggtgtt aaaatggaag atgatatgtc acctgctgaa acagaaggtg agattgttta
    15901 tgacgatagt tttgatgatg acattgatat taataatata gtaactatcg aacacaatga
    15961 taaaaagata gataaaaagg atgacaaaaa gaaagaaaaa agttttgata ttgaaaagct
    16021 taaaaataac gataagcgct ggcatctttc taaatataca ataaaaaaaa atgataatat
    16081 atggaagatt gcaaaaaagt ttaatgttaa ttataaatta ataattatgg ctaatgaaat
    16141 tgataaccct gatttattaa aaccagggaa aactataatg gttcccaata aaaatggagt
    16201 attgtaccag gttaaaaagg gtgagtcact atatgtaatt gctcgccagt ataatgtttc
    16261 tcctgatagg attataaaac ataatgaatt gaaaaatgct aataaagtac tgtctggtca
    16321 gaaaatattt attcctgatg cagtagaggt gataaaaaaa aatcccggtg atgtattgag
    16381 agataataaa tcaattgcaa aaaataatgt taccgggaat attaataaat caaataatat
    16441 aaatctttca ctgttgtggc ctgttcaggg taaaattact tccggatttg gaactcgaag
    16501 gaatccatta ggtaaaggta agcaatttca ctgtggatta gatatcagtt gtgaacctgg
    16561 tactaccgtc aaagctgctt tagatggtaa tgttatattt tcagggtgga aagatggcta
    16621 tggtaatgtt ataatcctta agcatgataa aggctatata actgtttatg gacataatag
    16681 caataattta gttgtggaag gcgatactgt agaaaaaggt caggaaattg caaaaagtgg
    16741 catgacaggt tcagttacag gagcacattt gcattttgaa atccgcaaat atttaactcc
    16801 tttaaatccc ttgctatatt ttaaggaaaa atagataatg aaaaattttc catccataat
    16861 aattataaaa atattttttt tcattacatt ttgtttactg attgagataa caatttttcc
    16921 ggatactcat atgaaatatg ataatcccaa tcagattatt aaaatatttt atactcaaaa
    16981 tgattatgtt gcatggaagg cgatagttaa gggtagaatt ttaagtattg gtgtaagaga
    17041 agatgctgca aaacaggagt taacacgtac tgtacaacca aaaacgaaaa taactgcacg
    17101 attatataat accgaaggaa tacataaggg tactacactg tttattatta attcaaataa
    17161 tttgattgtt tcgcgtttta ccgttgaaaa catatttcac tcacaatcat ttggggatat
    17221 gcttattggt tatggatatt ttaggttgtc aactattggt gatagggttg tacaacgttt
    17281 tgatggagca tatccagaat atgctaaagt ccataaagca agaggcgatt attatgttaa
    17341 tataggtgat tatggtaatg ccataaaaga atataatgaa gcaatcataa tggatatgaa
    17401 ttatcctgaa acgcatttag cattaggaaa actatattat aatgataatg tttatcagtt
    17461 tgcatttaca gaatttgaac aagcatataa aaataaagat agaatggtag ataaacagga
    17521 ttattatgaa ttgttatatt taatggctaa aacacgatat attgaagcat atgaaaccaa
    17581 catacctaag aaattaaagg aacactatct taaagaaggt atgaaatatg caaatgaggc
    17641 actttcaata aatcctgaag ccgtggaact attgtatatg ctgggagtta tgtattataa
    17701 aagtcctgaa ccagatgatg tggctgctaa aaaaacattt gaaaaaatta ttgagcttga
    17761 ccctacacat attgatgcat acattgcctt atcggaattg tatctaaagc ataaaaataa
    17821 agataaagca agttactatt cacaaaaagc actgacgata gaccctgcaa atgaacgtgc
    17881 acgatttctc tacaggatga caaaggaaaa ataataaatt aagcatacca ttatattgta
    17941 tatgttaaag tatataattt tttgtaaagt tgcgtgtatt gtgctctttg aattgatatt
    18001 caaaaaaatt gacttgactg taagtagcat tttttactat gattcttgcc tgtaccatga
    18061 aacaaatatt ttataataaa aaaaataaat aataggtaat agtatatgat tactgtaatg
    18121 caaaatgaaa tagtgcaatc gcttttgccg ttgattatca taaatgcaat tactcttttg
    18181 agttgcatta tattcatttt tatttatcca cgaaggaaaa aagatcctga gatagcaagt
    18241 cgaatgcatg aaacattttt aggtttattt ttcagagaat attggtactg gcttaataag
    18301 ccttttatta atttctttat ttattttaaa attaaaccta atgcgattac aactctatcg
    18361 cttattacag cttttataag tgcttattat ttttatagtg gaaattttgg tttggcagga
    18421 tggatattaa ttgttagtgc tacccttgat attattgatg gaagggttgc aaggagtact
    18481 aacaccgtta caaaatctgg agcatactgg gattcatgtg ctgatagata cagtgaaggt
    18541 gttgtgttat taggcattgc actttattat caaaataatt ttattgcatg ccttgccact
    18601 attgctacat taattggttc ggaattagta agttatacca aagcacgagg tgaagctatt
    18661 ggcataacta ctacgcgagg tgttatgcag cgtgcagagc gcttgtttac tttatgcata
    18721 gtttcagtgt tgcatccctt tttccaggta ctatttaaaa atacaaatgt aaatcctgaa
    18781 atagttatga ttagcgctat gatactaatg gctgttgcta ctaatcttac agcaattacc
    18841 agactgcgaa ctattttcat ggagattaaa agacaagaga acaatgcatg aaaagacttt
    18901 ttcaattaaa acactgggat gtaaatgcaa tcaatatgaa tctgcacaga tagcctcaca
    18961 attgttagcc tctggtttta ctgctgtgcc atttggaagc ttagctgaca ttgttatcat
    19021 caatacctgt acggtaacgg ataaaagcaa taaaaagtgc cgcaactata tacggcaggg
    19081 tgcgcggttt tcaaaatcag gtggtgttat cgttactgga tgtatggtac aaacccataa
    19141 acatgaaatt gaaacaatgg atgaagttat tgctgcttat accaatgaac aaaaagggca
    19201 attgatttca ttactaggaa ctatcgacaa cacaaagcaa aagtctttaa atcattttga
    19261 aactaaaaca tctccatttg ccttacccta tatgcgtacc cgtggttata ttaagattca
    19321 ggatggctgt gacggtcatt gttcttattg tattgttcca gcagtgcgtg gtgtaccaca
    19381 atcccggcca atagatgatg ttatcaatca tgcccgctac cttattgatt cacaatgtcc
    19441 cgagattgtt ttaacgggta taaccattgg taaatatcat gataaaggtt actcgcttgc
    19501 gcagcttatt gaaaaaattg ttcaactgga tggcaaattc agggtcaggg ttacgtcggt
    19561 tgagcctgtt catgttgatg atggacttct tgccatgctt tctcaccata aggtttgcaa
    19621 tcatatacat ctgccgctgc aatcgggctc tgatgctgtt ttaaaacaca tgcgtcgtcc
    19681 ctataatttt acttattaca tgcattgtgt tgaaaaaatg aaaagggtac tgcctgatat
    19741 agctattggt actgatataa ttgtagggta tccagttgag ggcgatagtg actttgcatt
    19801 gaccttgcag gctgtcactg agtgccagtt tgcatatatt catcagttta cctattcgcc
    19861 gcgtgaaggt acttgctcca gtgaatgtaa aactatccct catgacattg ttgcgatgag
    19921 agcagagcaa ttgcgaaatg ctgcacaaaa atgttcatta cagtataaaa aacaatttat
    19981 ccatgcaaca cgggaagctg ttattgaaaa aggtaatgat agtatagtag ccctcacaga
    20041 caattatctc aaggtaaaac ttgaaagtaa tcatcatacc agaagtaagg taggacagct
    20101 attacctgtt accatcacca ctaattcaag tacaggtttg cagggtaaaa ttgcctgtga
    20161 taactaatac tatgcatcga atcacaggtc aggaactaca aatcatagta aactaacaaa
    20221 tcacggtggg cattttgcgc atatagactg gaataacgaa gtagataggt atctatcgcc
    20281 acgatcgggc agtatggtga caacattacc atgcttcata ccttgtgcaa ccttcacagc
    20341 gccagcaaca gcagcaccgc ttgacatacc acagaataat ccttcctgta aggctaattt
    20401 tcgtgaatac tcaaaggcag tttcatcatc aatggtgata atatcatcta ataagccggg
    20461 atcataaatt tcaggaacta tcgcctcctg catatttttt aaaccctgta tggaatgacc
    20521 gggtggaggc tcaacaccaa taatcttgat tgaagaatta tgttctttaa ggcgacgggc
    20581 aactcccata atggtaccgg tagtacccaa tcctgctacg aaagcgtcaa tagcgcctgc
    20641 tgtttgttga agaatttcaa cgccggtagt ttcatagtgc gccatgacat tggcaggatt
    20701 tgaaaattga ttgggcataa aataggttgg gtcttccatg cacatcttct gagcctggat
    20761 aattgcacca tcagttccca tacaaccgtc tgtcaatatc aggtgtgcac caaatgcaat
    20821 taaggtgtgc tgacgttcaa cacttacaca tgaaggcatg cacagcgtta cctgaaaacc
    20881 taacaccgag ccaaccatag ccagaccaat ccctgtgttg ccgctggtgg gttctaaaat
    20941 tattttatca tgagttaacc tgccagattc aatagcggac ttaatcatgt accatgcggt
    21001 tcgatctttt atagatccgc tgggattggt accttcaaat ttagctaaaa ttgttgtatt
    21061 ggaaggtgaa agtttcgtaa gttcaatgag aggtgtattg cctattgcag caattaatga
    21121 atgtgctgga agctgaattt tattattaat tttatgcatg atggctacat tacagtatca
    21181 caaataataa tggtaatggt atataaaaat agtatatacc acaatcacta caaagtatat
    21241 cacatgggtg atagaaaata catataattt taaaagcaag catagagatt ttttataacg
    21301 taaatgatgg aacaaataaa aattaacaca tatgagcggg tgatgggact cgaacccacg
    21361 gcgttcagct tgggaagctg acattctacc actgaattac acccgctttg ataattgtga
    21421 ggtaaccata acaaaagggt attaattttg caattatttt ttggaaagta tgacatctac
    21481 atcattgcag aagtaaaatt tatggcttta tagtttgttt caaatatttt ttcctgtatg
    21541 ttcaatggga acatccttcg taccgtgaaa aggcaaggtt aaaggatgtt gcggagttaa
    21601 atccgaccga tatgcctata gacagtatgg atcgttttgg ttcctcaagc agcattttct
    21661 tcgcttcttc tattctgaat tcgttcacaa acccagaaaa attcttattc aacttctcat
    21721 ttaatatccg ggagagttgt tgttgcctta tgtcaagttc atcggcaagc tgcgacaggg
    21781 atagctcctc gtctgcaaaa gctttttcct cgttcatgat gtacataagt agctgtaccg
    21841 ttctgtcaat gtcgagtcta tttatctttg agtgttcata ccgtgccttg agggtttcag
    21901 cctttaataa acgattaaag tcaggatgac gctgagtaga tagaaacact ccggtaaggg
    21961 tagcatttgc catcagcact gaccaccttg taagttcggt tgaaaaaaat cttccgcagc
    22021 cataagtgct gttgaaagga taatacagta cgggaatagc ttcttacaaa cgccccattc
    22081 atgtagagtt atggcccgtt tgccagcaat tccctatgaa caagacaggg aaggttgatt
    22141 taatggcact tatagaaaaa gcaaaggaaa taacggaaga gttgcggaaa caggggaagt
    22201 gggatgcaaa gtaacggtag taattgacat acatgacggc gggagttgta ccgccgtttt
    22261 tttttttgat taaaaagtgc atcacggtat ttcattataa ttctttttta tttcattatg
    22321 gtaaaaggag tgccatttta aatggcattc tttattttgt actatcaatt atttaatctg
    22381 tttcaccaaa aagatatggt atactatatt tgtagtgatt ccaataatgt atgttttata
    22441 attttagttg aattttttat agtggataaa acattccatt gtataaataa ttattaatgg
    22501 tagcatatcg tgttaatata ataacattat ttattacaaa taagtaaaaa agaataggag
    22561 gtttaatttt ttaattgaaa tataatgtta tttttagagg attgtacata gtaatcttta
    22621 gtagttatat ttattgtatc aatcattaaa tttttaatat ctataatatt attaataaat
    22681 attaaaagag gtaatatggc aagcttagat gcggttgtta aacaggttgt ttcaaagtat
    22741 ggtaacgaca aaacccgtct tatggacatt atttctgatg ttcagaatga actgggttgt
    22801 gtgtcggatg atgctgttgt tcagattgcc agggcattaa atatctcaac agtggatgta
    22861 gatggtgtta tcaccttcta tcactttttc actaaaacgc ccagaggtaa atatacaata
    22921 tatctgaata acagtgcagt agctatcatg aagggtcgtg ctgctgttgc taaagcgttt
    22981 gaagaagcag cgggttgtac gtttggcaag acaacttcag atggccttat agggttgtat
    23041 gatacttcgt gtatcggaat gaatgaccag gagcctgctg caataattaa tggggttgta
    23101 tttactcatt taagtaagga taaagtacaa gatatcgtag caggtatgaa agctggtaaa
    23161 aagattaacg atcttgtgaa agaatacgga gatggagcaa atcaatcaga gcttgttcgc
    23221 tctatggtaa agaacaacat tatgaggaaa ggtcctgtat tgtttgcacc atatgaaaag
    23281 ggagcagcaa ttaaggttgc acttgcaaag aaacctgaag atgtaattga agaagttaaa
    23341 attgctaatt tgcgcggtag aggtggtgca ggattcccaa caggtatgaa gtgggaattt
    23401 tgcagaaaag ctcagggtgc aaaacactat gttgtatgta acgctgatga aggtgaacca
    23461 ggaaccttca aagatagggt gattttaact gaattacctc atatgctttt tgagggtatg
    23521 gcaattgcag gttatgctgt tggtgcaagt gaaggagttc tttatttgcg ggctgaatac
    23581 ctgtatttga aaaagtatct tgaaaagata cttgatgatt taaaagctaa aaatatttta
    23641 ggtaaaaata ttgccggtaa aggatttgac tttgatatta gaattcagat gggtgcaggt
    23701 gcttatattt gtggtgaaga atcagcactc attgaatcag ctgaaggaaa acgaggagaa
    23761 cctcgaaaca gaccaccatt cccggtagaa aaaggatatc ttggatatcc aacaacagta
    23821 aataatgttg aaactctttg ctgtacaaca cgcataatgc tggaaggcgg cgaatggttt
    23881 gcaaagatgg gtacggcgca atccaaaggt accaagctat tgagtgtctc aggagattgc
    23941 gataatcctg gagtatatga ggttgaattt gggacaacta tctcaacatt attagaatta
    24001 gttggcggaa gaactgcaaa agcagtgcag gttggtggcc cttctggaac atgtatagga
    24061 aagaaagatt ttggaagaag gatttgtttt gatgatttag ccacaggcgg ttctataata
    24121 ataatagggc caaaccgtga tttgcttgag atagtacata attttatgga cttttttgtt
    24181 gaagagtcat gtggatggtg tgttccttgt agagcaggta acgttatatt gaaacaaaaa
    24241 ctggaaaaga taatatccgg taaagcttca gcaaaggata ttcaagaact ggaagaatgg
    24301 tgtagaatag ttaaagctac aagccgatgt ggtcttggac aaacttcacc taatccaatt
    24361 tatacaacaa tccagaattt cagagagata tatgaagcaa aagttaagaa ggatactgat
    24421 tatgtatcca catttgatct tttagctgct gtcgaagaat catgtaaaac agcaggaaga
    24481 attccaaagg tagaggaaca ttaatatgag tacaataaat tttactatag atggaaaatc
    24541 ctgtaaagca aagccaggtc aaactattgt agaagcctca aaagataatg gcatttatat
    24601 tcctacttta tgccattttg aaggtttgaa acctgctggt acatgtcgta tttgtacagt
    24661 taaggttggt ggcagaaata tggctgcatg tacaacacca gtaacagaag gcatggttgt
    24721 agaaaacaaa acatctgaac ttgaagatta tcgcaaagcc attgttgaaa tgctttttgt
    24781 agaaggcaat cacatgtgtc caacatgcga aaaaagtggt aattgtgagt tgcaggcttt
    24841 aggatatcgc tataaaatga tggcacctcg attcccattt ttgtttcctc aacgaacgct
    24901 tgatgcttct ttgccaaaga taattattga acgcaataga tgtgttcagt gcttacggtg
    24961 tgttcgtggt attacaacat cagatggtaa gcgtattttt ggtatggtta acaggggtgg
    25021 tcatgtagaa attacagtgg ataaggaatt agctgccaat ataaccgatg atatggcaaa
    25081 aaaggctatg gatttatgcc ctgttggtgc tatcttaaag aaggaagtag gttttgcaat
    25141 accaattggt aaacgtaaat ttgatgctaa accaataggt agtgaaattg aaaaaatgta
    25201 atgattatca taatgaagat tgatggagga atacaatgag taaacccgtt gtcgcaacag
    25261 cttcactggc aggttgtttt ggatgccata tgtctttact ggacattgat gatagaattt
    25321 taaaattaat tgaattggta gaatttaata aatctccaat tgatgatatc aaaacattta
    25381 ctaagcagtg tgatattgga ttgatagaag ggggatgctg caatagtgag aatgttgaaa
    25441 atttgaaact tttccgaaag aattgtaaga tattaatatc agtaggggaa tgtgctataa
    25501 tgggcggatt gcctgcaatg cgtaatggca tttcagtgaa agaatgttta gaagaagcat
    25561 accttaaatg cccaacagtt gatgataata taattccaaa cgatgatgaa ttgccaatga
    25621 ttcttgataa agtgtatcca tgccatgaaa tagtgaaaat tgactatttt ttgccgggat
    25681 gccctccacg tgcagattta atctgggaag cattggttgc acttctgaca ggtaaagagt
    25741 tagatttacc atatgaagtt gttaagtttg attaattttt ttcaggagat gaaattatat
    25801 ggctagaaaa ataacgatag aacctgtaac aagggtagaa ggtcatggta aagtgaccat
    25861 tcagcttgat cctcagaaca atgttttgga atcaaggttc catattgttg aattcagagg
    25921 atttgaacgt tttgtacagg gcagaccata ctgggaagca cctgttttag tgcaacgcct
    25981 ctgtggaata tgtcctgtta gccatcattt agcagcagct aaagcgatgg atgttattgt
    26041 tggtgctgga cctgatggat taacaccaac tgctgaaaag atgcgcagat taatgcatta
    26101 tggacagatg ttccaatcac atgcattaca cttttttcat cttgtatcac ctgatttact
    26161 gtttggtgta gatgctgatc cagctatcag aaatgttatt ggtgttgctc taaaatttcc
    26221 cgatttagca gttcagggtg ttatgatgcg taaatttggg caggaaataa ttagagcaac
    26281 tgccggtaag aaaatacatg gtacaggtgc catccctggt ggtattaata aaaatttaag
    26341 tattcaggaa cgggatgagt tccttaaagg tcaagaccca atgaatatta caaagatgat
    26401 agagtgggca aaggctgctt tagatatatt caaagattac cataaaaaga ataaagattt
    26461 tatcgatagc tttgcaacat tcccatcaag tcatttaagc cttgttagaa aagatggagc
    26521 tcttgatctt taccatggtg ttttacgagc agttgattct gacggtaaga aaatattgaa
    26581 tgatgtggac taccaggatt atttgaaata tgttggtgag gaagtgaaat catggagtta
    26641 tatgaaattc ccatatctca aatcgctggg taaaaaagat ggttggtaca gagttggtcc
    26701 gctggcacgt ctgaatactg cagattttat accaacgcca ttagctcaaa aagaatttga
    26761 agaatttaaa gcatatacaa agggcaaacc caataacatg tcaatgcata tgcattgggc
    26821 acgattgata gaattattac attcagctga aatgatagaa cagcttttaa aagattcaga
    26881 tcttcaaggt gataaactgg ttgctaaagg aaagaaagtt aaagaaggcg ttggcttaat
    26941 agaagcacct cgaggaacct tattccatca ttatcgtatt aatgataacg atcagattga
    27001 aatggctaat ttgatagtat cgacaacaaa caacaatgaa cctatgaata gagctgttaa
    27061 ttgggtagct atgaatacca tcaatggaaa gaaggaaata actgaaggta tgctcaatgc
    27121 agttgaggta gcaatacgtg catatgatcc ttgcttaagt tgtgcaactc atgcaattgg
    27181 taatatgcct cttgaagttt cacttgtaga tgctgacggt aacatactgg atcaaaaaag
    27241 gaaatagcaa tgccagatgt taagtgctta atatacggat atggtaatcc tggacgtcag
    27301 gatgatggat tgggtataga actggcaacc atggtagaaa ataatccaca attaaaacat
    27361 catgttgatg ttgattataa ttatcagctc aatattgaag atgcattgac tatttcagaa
    27421 tatgataaag taatatttat tgatgcagca tttaacattg ataaaccatt tgtattttat
    27481 acaattcagc cggcagatac cataacgttc actacgcact atttgtcggc tgaatctata
    27541 gttgctcttt gcaatgattt gtataaaaag caacctgaaa gttatatgct tgcaatacag
    27601 gggtataact gggaattagg attaccgatg tcagaacagg caaattctaa tttgcagctt
    27661 gcatataatt ttatttcaga agttattttg aatcgttttt catgttgtat tacaggttaa
    27721 actttatttt agattaaaat caggagtgat agtatggcaa gtaaaaaact gatattagta
    27781 attgatgatg atccagatat tttagattca attaaagcta ttcttacagc taatgcattt
    27841 gatgttgtta ctgcaatgag tggcaaggaa ggcatcgaag ctgtaagtaa aaccaagccg
    27901 gatttaattt tatgcgacat gatgatggaa aggatagatg ctggtacaaa agttgctcag
    27961 gaattaaaaa aggataaccc caatttacct atttatctat taagtagtat tggaacagcc
    28021 actgcaacca atattgaaat tgataaatta ggttttaatg gtgtttttca aaaaccagtc
    28081 aatcccgata atcttgttgc aacaattaag aatgtgctaa atatatcata atatgcattg
    28141 cttaatcatt tcgcttttca atacaaaagc gggtttaatt aacccgcttt tgtattaact
    28201 aatattggtg atagatacta tttatctaaa gcctttactg cagggagcac tttcccttcc
    28261 ataagttcta gaaaagcacc acctgctgtg gaaatatatg tcatcttatt tgcatagcct
    28321 gaagcattta ttgcagcacc agtatcacca ccgccaataa cgctggtaca agctgactca
    28381 gctaccactt tggcaatttc attggttcca tcagcaaaat cttttgtttc aaatgctcct
    28441 aatggcccat tccatattat tgttttggat tgtttaatga catcggaaaa ttttttgcga
    28501 gttattggac ctatgtcaac accctctttg ttatcaggaa tattattaat gggaactact
    28561 tctttccctt cgggactatc aaatttgtta gcaacaataa tatcttcagg taacatcaaa
    28621 ttaacattat atttctttgc aagctccata atttcttttg cagtagcaag gtgatcatct
    28681 tccacaactg atttgccaac attgtagccc aatgctttta aaaatgtata tgacatgcca
    28741 ccgccaataa gaagattatt aactttagga ataatatttt ttaaaacatc aatttttgta
    28801 gaaattttga tgccacctat tatagcggtt actggttttt caggatgtac cagcgctttc
    28861 ttaaaatact ccagttcttc tttcattaaa aagccagcaa cacattcttt tacataccgc
    28921 gttatagcct catttgaagc atggcctcga tgagcagtgg caaatgcatc gttaacatat
    28981 acatctgtca atgaacccaa aagcttacca aaactttcat catttttttc ttcttctgga
    29041 taaaatctaa tattttcaag taaaacgaca tcacctggtt ttagtttgtt tatttcttcc
    29101 tgaattcctt gagttatagg tttgtctata aaaagtactt ctttttttaa caattcagat
    29161 aaacgtttag ctactggttt tagtgacatt tccgggacta tctttccttt tggtcttcct
    29221 aaatgtgaaa caagtataac aatggcattt tgtttaaggc aatattcaat agtaggtaat
    29281 gttgcggtaa ttcgtgtatc atcagtgatg ttcatgtttt tatcatacgg aacattaaaa
    29341 tcagctctaa tcataacttt tttattgttg acgttaatct ggtcaagata tttaattgcc
    29401 atatttcacc tcataaaata ttattttaaa taattctatt ttaattcaat atcaaaaaat
    29461 tattaaaaaa aagtcaaata gttattgcga tagttccttg cgttttttcc gtataaccat
    29521 aaattttgat attatgatag acagttcgta taataacatt aatggaagag ctacaaggga
    29581 tagtgtcaat ggatcagttg taggagttat tacagcagca aaaatccata taagaactat
    29641 tgcatatttg cgtttgctaa ttaaaaatga tggtgaaaca attcctatgc gtgtcaatat
    29701 cataatgata ataggcattt caaatgtaat acctattgca attgaaaaaa gaacagcaaa
    29761 gttgaaatat tttgatgcat taatcatatt gtccatgtct ggaggtgtaa agtttaaaag
    29821 aactttaatt gaaagaggta gaagataaaa gtatgtcata gataaaccac cataaaataa
    29881 aaaaattgct gctacacttg aatatttagc aaatttacga ttttcaatgg cgacagccgg
    29941 cttaatatac tgccataatt gatatactaa aagtggcaaa cctattagca aaccacttac
    30001 aaacgatgct tttagcctca aggtaaaacc ttcaacaaga ttaaatatat ttagtttata
    30061 tcctgaatca ataaatggtc gattaattgc atccagaaga atatccgaaa aataaaatcc
    30121 tatgatggtt aatcccaaaa tagttatcag gactattaat aacctagaac gaaactcatc
    30181 cagatgatct actatattca ttggtttatc acctcgacta agtgctgcag gatcaacttc
    30241 attttcttct atttcagatt tagaagtttg atcattatca gcttctttaa tcagttcatc
    30301 tatcaagttt ttcttttctt gtttttgttg attgttgcta agatcttcac tgttagtgga
    30361 tagggatttc tttttccttg tgactttagt ttcttttttt tgggaatttt ttcttttctt
    30421 cccggaatta tttttggtgc tatcattttt tggtttttgt tcacgtgcca atgtcatacc
    30481 tcaatatttt aaaatataga attatgggcg cttctaaaaa tgtttttcat aatcattatt
    30541 gaaaaaatca tataccattt taccaaacgc ttgtgcccat aaggggacat caaaaatata
    30601 attattagaa gctgctgtat aattgtataa gataacttaa ttatctaaaa aattgtcaag
    30661 ctattataat aattaaaatt taatatattg agttattttg ataaaacttg acaatatact
    30721 attaatatag tatcatacca tatcttaata atactattaa ctaagtgttt agtctaaaat
    30781 gtttaatgat tatacacatc ttgatgctgt tgttattcct attattattt gcgataataa
    30841 aggcaaagtt aagtatgtaa ataatgcatt cagaacaact aaatattctc attcaagtga
    30901 caatgaacta ttgaaccaac aatctgtttt tacaattttt acacttaatg aaaatcaaaa
    30961 aagtgaattt aaaaaagtta ttaataatga attaaacaat attacgatta tattagatac
    31021 atttcaatta ttgataagta agataaaaag aaatcgcaca ttagttcaaa tttttaaaac
    31081 acataaggat tttaatactg cattacatat gtataatgca aatgtagata gttttaagaa
    31141 tacttttgct ccattttttt ctgcttttga tttacttgat gatctcatcc atataactga
    31201 taaagatgga tgtatcatat atgcaaataa agcactcaat aaattatatg gcgatattaa
    31261 agggcaatat tgctattcgg tatttagtaa atatgataat tattgtcctg actgcaagat
    31321 gagtttagta ataaatggga gtaccatacg gtatgaagca aaattagaaa agaatggtca
    31381 atactatgat atcattaatt ctccatttat cgattttgat ggtaatattt ataaaatatc
    31441 tataatgcgt aaccttgaag agattataga taaacaaaat actctcttat tatttcagaa
    31501 tgcgattaac agatcaagaa atcttgtaag ttttttcaag gaaaatggtg aaatttcata
    31561 cgcaaatgat acttttatac aatattttgg agaacaatat aaaaaagata tcaatttttt
    31621 aaaactcata aattgtacta acgatgaatt attttatgat gtagttgata aagaaatatc
    31681 cttaacactt aataataatc ttatatttct taaaatattt atttatccca ttcattctag
    31741 tgataatgaa attatttttg tttttatagc aagtgatata accaatttga aaaaattaga
    31801 aaaacaactt gctgttgagc gcaattactt taaaagtata atcgataata ccatagatgg
    31861 gttttttgtt attaataaaa atcataatct cattgattgc aaccctgcct ttttaaaaat
    31921 gtttgatcta tcatatgata attttaagaa tattcgcatg gaagatcttt ttgattcaca
    31981 atataaaaag gattttgata aaaaaattaa ttatttgaac aaaacaggaa tatcacagat
    32041 tacagaagtt agccatacta aaaatggttt taaaaattat attataagtt taaataaatt
    32101 aacttcgtca gaaatagatg atgcacaata ttatggattt gtaaaagata taacagatat
    32161 tacccggctg cataaaatta ttgagaatga aagaaattat aaccggcgga ttattgaaac
    32221 ggtacagctt ggatttgtag taataaatga atttggtgaa attattgact ataataatgc
    32281 atttcttgat ttaatagggt gtaatggtaa aaatgtaaag ggtgaaatat ataagtcttt
    32341 catcataccg gtatcaaggg atataatgga aaatttcaat gtagtatcat caaataatcc
    32401 aattgaattt gaagcaatta ttcaaagttc cgacaataat aataaaaatg ttataatatc
    32461 aatgtctaaa ttatatgata tactgggaaa ctatctggga aattttgctt ttatacgaga
    32521 tattactcat caaaagaaaa ttgaaaatga gatcctatat caaacaaata aggtaattga
    32581 gttagtaaat tcattaaccc aatttactgc caatgttgta cgaatagata cgatagataa
    32641 cctgttattt gcaatatcat catttataca acaactcatt aacccggatt atacagaaat
    32701 ttttattaat aacaatacca tatttgtaag tcataagttt aataatggcg tacatactgt
    32761 taataatact gaaataagtc aaaagacgtc ttttgtagtt caaaaattaa caaaaaataa
    32821 aactatacta tatatctcaa atccattaat agaattaaat gatgaagatc aattgcattt
    32881 taaaaatatt cttgaatttc aaaaaatcat attgatccca atattcattg gagatgaact
    32941 taaagttata ttgctcagtg catttaaaac gaacgaagaa aagatagata atgtcatatt
    33001 aactatatta acaggtgtta ctaatttatt agctattact ttagaaaaaa ttatatcagt
    33061 tgctgaacaa aataaaatgc gtgaggcttt agatagatat gataagttag tagccattgg
    33121 tagaataata gctggtgtag cacatgaaat taataatcct ttatcgatca tgcagcttga
    33181 tattgctgaa ttaaatcaaa aattacaagg tgatgagtac gataatgaaa taagagatat
    33241 tattaaatcc ctgtttgaag aaatacaacg tataagcaac attgtgaaac aattaaaaga
    33301 ttattctaaa cctgcaacgt taaatgatga cattattaat ctgaatgaaa tattcagaac
    33361 atatccatta aaaattcttt ccaagaacat tcaaaagaaa ggaataccca ttgtaattaa
    33421 tgtaccaaaa cagaaaatat ttataaagat gccgttaact cgtttgatac aggtcattat
    33481 gaatttacta accaatgcag aagatgcagt tattgaaaaa ggggagggta gtatcatcat
    33541 aactatctca cagattcaga aagatgatat atgctatgcc tgtttatcaa ttcaagatac
    33601 cggcattgga attgatgagg agaatatgcc tcttattttt gaaccatttt ttactacaaa
    33661 acctaaagaa ggcactggat tgggattatc tatatcatat tcaattataa aaagttataa
    33721 tggggaaata gagatcaata gcaaaaaaaa tatgggaaca actgtgtcag tattgttgcc
    33781 tgcatataaa aaatagtacc tgtatagttg catcggctac taaaaaaaat atctattatt
    33841 acaaggtttc aattatttac tttatgatat ttactttatg ttgatagtaa agaaataaaa
    33901 tcaatatata ctaattcaac agaataatat aaaagcaata atagaataaa tagtatagta
    33961 accattacag gaactgaatg gaagtgcccc tgcaatgttt taatatcgat aatgatctgg
    34021 tttgtatggg ccatcaatat taactcccag atatgaagct tgcttttgat ttaaacgagt
    34081 taatttaact cccaatcttc ccagatggag acgtgcaact tcttcatcaa gtttttttgg
    34141 tagtgtataa acttttttct ctaatgcttg atttgttgca agctcaatct gcgctaatgt
    34201 ctgattggta aaactattac tcataacaaa gcttggatgt ccattagcgt ttcctaaatt
    34261 aaccaatctg ccttctgaaa ggacaatgat agaatgtccc ttttccagca tccacttatc
    34321 aacctgtggt ttaatattaa gtcgtttaca tttatcatta ttttcaagat agctcatctc
    34381 tatctcgcta tcaaaatgtc caatattaca tacaattgct tcatctttca tgcgttccat
    34441 atgctcgcca cgtattacat cacaacagcc ggttgctgtc acaaatatat ctccagattc
    34501 tacaacatct tcaagggttt tgacctcata tccttccata gcagcctgca atgcacagat
    34561 aggatcaatt tcggtaacta tcacccttgc cccaaaacca cgcatggatt gtgcacaacc
    34621 tttaccaaca tcaccgtaac cacatacaac aacaactttg cctgcaacca ttatgtccgt
    34681 agcacgcttt attccatcgg ctagcgattc ccggcatcca tacagattat caaattttga
    34741 ttttgtaact gaatcgttta cattaattgc agggaaaaga agctctcctg atttttcaag
    34801 ttgatagagg cggtgtacac ctgttgttgt ttcttcggat actccacgga tttttgaagc
    34861 gatagttgtc cagcgatttg gatttttaga aacagatagt tgcaacctgt caataaggca
    34921 ttgcatgtcc ttgctgtcat aatttttatt gataattgat gaatcgtttt ctgccttgac
    34981 acctaaatgg acaaacagag tggcatctcc gccatcatca acaatgcaat caggaccact
    35041 gccatcaggc cagatgagtg cttgctccgt acaccaccaa tagtcttcta atgtttcgcc
    35101 tttccatgca aaaactgcgg ccgtaccagc ctgtgcaatt gctgcagcag catggtcctg
    35161 tgtagaaaat atattgcatg atgcccagcg tatatcagca ccaaggtgat aaagtgtttc
    35221 aataagcatt gcagtttgaa tggtcatgtg cagactgcca cttattcgca aaccttccaa
    35281 tggtttttga tttccatatt ttttacgcaa tgccataagc cctggcatct cgttttctgc
    35341 taattggagt tctttgcgac caagctctgc cattgtaata tctttaacct taaacggcaa
    35401 tttatgatca tattcagcaa tattcagcat cccctttcct gaaggttgag tcatacaaca
    35461 cctcacataa ttattaatta ttatttaaat ttttttgtac tatttcggca tttaccagct
    35521 aaattgtcaa tataattatt ttatcctttc atgtcgatgt ttattatatg aaatgtaaaa
    35581 acatttttta tgtactatcg cttatactca tattatcatt atcttcttgt actccgtctc
    35641 aatggcatta tcgtaatata ccgttaaaac gaaaacaaat tataaatact gcaacaaaat
    35701 acataggtat accttatcgc catggtggta gcaatccctc aggttttgac tgttcaggat
    35761 atgtgtacta tgtatataca aaaaatggta tccctattcc acgaagtaca acaatgcaat
    35821 ttaaaaaagg aaaaaaaatt ccttttagag tagcacagcc aggtgatctg gttttttttt
    35881 ctatccagag ttctggtgtt tcacatgttg gaatattttt aggaaatgat acatttattc
    35941 atgctccaag tagtggaaaa gctatacggt atgatttgat aagcaaccca tattggaaaa
    36001 agcgttttgt agcaacagtg acgtatttaa acaattatag tcagttaaac gaaatgaaaa
    36061 ccattggttc atcatattct gaagaacggt ttgaatggaa gtaaaaaaaa attgaaatat
    36121 gaataaatgt tgacaaaatg taataataag attattttag tagaaccgat caggataggt
    36181 tagttttata tgcaaattgg aatttatccg ggatctttcg atcctttaac gtgtggacac
    36241 ctggatataa ttaaaagagc aacacagttg tttgatagat tagtagttgc agttgctcgc
    36301 aacagcgaaa agtttcctct tttttcggtt gaagaacgtc ttgcaatgct ttctgagtgt
    36361 tgcagggata tatctaaagt tgaattaaca agttttgatg gactccttgc agaatattgc
    36421 aagaaaaaca atattaattt tattgttaga ggattgcggg cgatagttga ttttgaatat
    36481 gaatacgcca tagctttaat gaacaaggaa atagccccta cagttgagac catttttttg
    36541 atgtcaaaga gtgaattttc ttttgtatca tcaaaaatgg ttaaagaggt ggcaggctat
    36601 ggtggagata tatcccgact tgtcccacaa tttgtgaata agaagctcaa ggaaaaattc
    36661 caatcctgat gcaacctgtc ctgaagctac atccgtagca gcaaaagtta tatttaaaat
    36721 catgtaagga ggattattat gagtgtactt actatcctcc tgattgttgc tctgccatta
    36781 gcggcagtgt caggctattt gttcagagca tacataggga agattaagct taccactgct
    36841 gaagcacagt caagaaaaat aattcaagat gctgtgaaag aagcagaatc aaaaagaaaa
    36901 gagcttttac ttgaagctaa ggatcaattg ctgaaagagc gtaatcaatt tgaaaaagaa
    36961 atgcgtgaaa ggaggattga attacaaaat attgaacgta agattttaca aaaagaagag
    37021 atgattgata ataaaataca gcaattagaa aaacaggaaa aagcagttca gttaaaagag
    37081 aaggaaaata ttgcaaaaga acaggagttg aataagcatt tagaaaaaca taaacaggaa
    37141 ttggaaagaa tttcaggatt aacccgcgaa gaggccaaac aactattgct gaaaaattta
    37201 gaaaatgagg ttagatttga atccataaag ctgattaata aagtagaaga agaagcccga
    37261 aggtcagccg ataaaaaagc aaaagagata gttcttgcag ctttgcagcg cagtgcttcg
    37321 gattatactc aggaaagtac tataacaacc gtttccctgc catcggatga tatgaaagga
    37381 agaataatcg ggagggaagg gagaaatata cgaactcttg aaaacttaac cggtgttgac
    37441 atgattattg acgatacacc agaggtggtt gttatttcag gatttgatcc tatacgtcgt
    37501 gagatagcac gcttatcttt agaacgctta attgaaaatg gaagaattca tcctgcaagg
    37561 attgaagagg ttgttgagaa ggtaaaaaac gaacttgaag aaaatatgct ggatgaagga
    37621 gaacatgcag catttgaatt ggggattccg ggtctttcca gagatgccct gtattatatt
    37681 ggaaagttaa aataccggtc aagttatgga cagaatgtac tatcacacag caaagaggtt
    37741 gcaaacttag ctgccatcat ggcaggtgag ttaaagcttg atgttgccat tgcaaaaaga
    37801 gctggattgc tgcatgacat aggcaaggga agcattgttg agggtgaagg tgctcatgcg
    37861 atagttggct cggaattagc taaaaagttt ggagaaaatg atattgttgt taatatcatt
    37921 gcatcgcatc acaatgataa agaacctgaa tcatttgaag cgatacttgt tcaggtagcc
    37981 gatgctatct cagcctcacg accaggtgca aggcgtgaat cactggatac ctatctcaaa
    38041 cggctggaaa atctggaaaa tatagcatat ggatttaagg gagttgaaaa gtgttatgct
    38101 atccaggctg gtagagaaat tagagttatg gtttctaatg agcaggtgac tgatgaagaa
    38161 gcaacaattt tggcacggga catagcctca aagatagaat ccgaactaaa atatcctggc
    38221 atagtacgag taacccttat acgtgaaacc agaatagttg attatgcacg ataatttgaa
    38281 tgtaactata cgacaagcaa tgtggtacat tgcttgtcgt attttataaa actcaatgga
    38341 atcactaaaa gtattattca taggcgatat tgttggcaaa cctggtaggg ctataataaa
    38401 aaattatctt agcgatatct gccaggaata ttcaattgga cttgtcattg caaattgtga
    38461 gaatgcagca ggtggcatgt ctattacacc ggagatagca aatgagcttt tttcgtatgg
    38521 catacatgtc cttacaaccg gtaaccacat ttttaataac cgaaacagta ttcagctact
    38581 tgaaacaaat acacattttt tacgtcctgc aaattttccc gatggtgttc ctggaactgg
    38641 atttacaacc ttaacaatca ataatattac catcgcggta attaacttaa ttggaaggat
    38701 agggatggaa cctgttgact gtccttttat taaatttaat acaatatatg aaaccataca
    38761 atcaacatct gatttaatat tcattgattt tcatgctgaa gccacttccg aaaaaaaagc
    38821 atttggatgg tttgttgatg gaaaagcaac agcagtatgt ggtacacata cacatgtcca
    38881 gactgccgat gaatgcatat tgccacaggg aacggcttat attaccgatg ttggtatgac
    38941 cggtcctttt gattcggtta ttggcatgga taaagattca tctattcata atttcatata
    39001 ccatacacgc ataaaattta aagttgcaaa tgatgatcct aaattaaatg cagtggtaat
    39061 cacctgtaat aatgtaagca aagcagttaa catacagcgg ataattaaat cttatgagat
    39121 atcgacaaca aatccttgac aatttatggc agggttaatt ttaatgatag cgatagtaaa
    39181 tatgtgatat aatacatttt aatatattat tttaaaaaag atactgtatg ttagatgata
    39241 ttaatggacc cgaggattta aaaaaacttt caatagggga actatcttcc ctgagtgaag
    39301 aaatacgctc acgcatactt gaagttgtat cccacaatgg aggtcatctg gcttcatcac
    39361 tgggtgttgt agaattaact attgccctcc attatgtatt taattctcca actgacagga
    39421 ttatatggga tgttggtcac caatgttatg cgcataaaat tataacggga agaaataagc
    39481 tatttcatac gttaagaatg tttcatggca taagcggatt tcctaaaaca tcagagtctg
    39541 tacatgatca ttatgataca ggtcatagca gtaccagcct ttctttagct ttgggagagg
    39601 ctattgctcg agatataaat tcaggtaatt atcagattgt agctgtcatt ggtgatggtt
    39661 cattgaccgg tgggatgtca tttgaagcat tgaatcacat tggacaatca aaaaaagata
    39721 ttatcattat attaaatgat aatgagcatt caatatcaag aaatgttgga gcattgtctg
    39781 aatatcttat gcgccttata acaggaggcg tatataacaa attaagaaga aaatcatacg
    39841 aatttatcca tagaattcca aagattggac ccatgctttt taatttattt gaccgcattg
    39901 aagctcgtat aaaagggatc tttgtgcctg gaagtttctt tgaagatttg ggaatacgat
    39961 attttggtcc aattgatggt cataatattc ctactttaat tgatttattc aataggattg
    40021 caaagttaaa caaaggacca aaaattatac atgtatatac aaagaaaggg aaagggtatg
    40081 ttcatgcaga aaaaaatccc tcaaaatttc atggtattgg tccatttgac attaaaacag
    40141 gtaaaccttt aaaaaaatct tctatcagtt attctgacat agccggcaaa gctcttgtgg
    40201 atgtagcaag acgagacaaa aagatttttg ccattactgc tgctatgaaa ttaggaaccg
    40261 gtttatccga atttgaagac cattttccac aacgctttat tgatgttgga atatgtgagc
    40321 agcatgctat aacattggct tcctcattag ccaaaaatgg atttaaacca tttgtatcaa
    40381 tatattctac ttttatgcag agagctgttg accagattat tcatgatgtt gcattgatga
    40441 atttaccggt taaacttctg atagatagag cagggattgt tggagatgat ggcgaaacac
    40501 atcatggact ttttgatata ggcattatac gaatgatccc aaattttgtt ttgctttcac
    40561 ctactaatgg aaatgagcta agggacatga tacactatgc atctatttat aataatggtc
    40621 ctatagctat acgctatcct cgaggagtcg atgataaata tgttcattct gcaaatagtg
    40681 agttgataaa attaccactt ttgaaagcat attataaatc catacagcca atgattgctt
    40741 tatttgctgt tggagatatg atcaaacttg ccactgaagt tcatatgaaa ttgaatgaac
    40801 acgatataaa gtcttcagta tatggaatat tgtccttaaa accattgcct attaatcaga
    40861 ttgaaatcat tttacagaat attccctatt atactgtttt tgagaattgt tatacaagtg
    40921 gtggcatttc agagtacctg aattctcaga ttaactgttc attgcgttat aaaaatatat
    40981 ataatgttgc tttcccagac aattttatac ttcacggaag agtatcagaa ttatttgcac
    41041 attatagtat ggattctgat tcaattacta ataaaattat cagtttaata catacagaga
    41101 aagtttctca tgtcaaaaaa tcgcttagat caatatcttc ttcaaaataa aatatgtgta
    41161 tcacgcgaaa aagctagaaa tctaatactt gcaggatgtg tcagtgttaa tggtcagatt
    41221 gtgctaaaac catcatttat tattaatgaa aatgataaca ttatattact gcaagcatca
    41281 caaagatttg taagcagagg aggcgaaaaa ttacaacgag ctcttgatat ttttgacata
    41341 aatatttcta acaggattgt tttagatttg ggttcatcaa caggaggatt tgttgattgt
    41401 ctgttgaaga atggagcaag aaaagtatat gcagtagatg tggggtatgg acagttagat
    41461 tattctctta gaatgaatcc caatgttatt gtcatggaac gccataatgc tcgctatgta
    41521 ataagagatg atttctctga taacattgat tttataactg cggatctctc gtttatttct
    41581 atcgctaaag ttatgaaaaa tattgtgact atttttgaca gtactatcga ttctgtacta
    41641 cttattaaac ctcaatttga agcagaatat taccagcata aaaaaggagt agtaaaaaaa
    41701 ccagaatacc attatgacat tgtgtataag gttatccatg aattaacatt gcttgatatt
    41761 caaataaaag gcttaaccta ttcgccaata aaagggccta aagggaatat tgaattttta
    41821 ttattgtgta caattcaccc tgacaccgat actaatatta aagatataca attattaatc
    41881 aaaaattgtg ttgatgaagc tcataaaata ttagattaat gtaacttttt atttggtata
    41941 gagaatatat taattgacga aaagtattga gcttgtccca gaatctgttg aaaatgagtg
    42001 gcatctcccg gggcatcaga gtataatgcc ccaaaacaat accaacaata ataggagata
    42061 ccacaatgaa gcacaaaaca gtagtcagaa aaaagcaacc acaaaaaatt gtatcagtgg
    42121 aaaatgattg gaaagaagca atcaaagcaa cattatttga gacggtgctg cgatttggga
    42181 tagcagcata ttgtgagatg gtagaagaag agataacggc actatgcggg gaacggtata
    42241 agcacctatt caagcgggaa tacacgcggt gggggaagaa aaacacctcg gttgtattag
    42301 gtggtaggaa gattgcattg cagcattcac gtatacgaga catacagaat aatggtgaaa
    42361 aagcgatagc aatagtagag caatacaaag ggaaagaggt actgtcgcag cgacaaatgg
    42421 aacagatgat tataggcgta tcgtcaagga aatatggacg ttcccttgag ttaggcaagg
    42481 gtgcagtaga atcacatgcc tattcaaaga gcacggtgag tagaaatttt attgcaaaga
    42541 cacaggagaa agtggaagca tggcgtcatg cagcgataac cgcagagtat ccatttttga
    42601 tgatagatgg catagtatat gctacaacaa ccgtattggt agcacttggc attgataggg
    42661 atggtacgaa gaaggtatta ggtgcatggg aaggctcaag tgagaatagt cgggtatgta
    42721 ttgatttatt gcagcatctg gtagaacgag gatttgatgt cacgaagtgt tttcttgtga
    42781 ttattgatgg gagtaaagca ttacgaaaag caattcatga tgtttttggg gatagcattt
    42841 tggtgcaacg gtgtcaggta cataaggaaa ggaatgtcat tgattatatg cccaaggggc
    42901 aacgggaagc ggtaaaaagg gcactcagtg aagcgtatag tgccgatacg tatgatagag
    42961 caaaaaggtt actggggaac attcatcaat ggcttgcaaa agactatccg caggcagcac
    43021 gttcacttga agaaggatta gaagaaacat taacgctgca taagcttggt gcgcacaaaa
    43081 agttacggaa aagtttgggg acgaccaatc ccattgaaag tttgaatagt ggtatacgca
    43141 ctgtaaccgg gagagtaaaa cggtggagaa atgctacgat ggtaatgcga tgggtatgga
    43201 gtggcataat agaaagtgaa agaaacttcc gaaagattaa cgggcatacg tacattgatg
    43261 cattattgct aaatattgca atgtttggga aaaaagtgat tgaagaaaat aataagattg
    43321 cata
//
LOCUS       DAOOJH010000014        42620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1011_length_42620_cov_9.995724, whole genome shotgun sequence.
ACCESSION   DAOOJH010000014 DAOOJH010000000
VERSION     DAOOJH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 42620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42620
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1011_length_42620_cov_9.995724"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2630)
                     /locus_tag="PLJ75_03575"
     CDS             complement(<1..2630)
                     /locus_tag="PLJ75_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPD04328.1"
                     /translation="MVLKKLTQIYASTDECKPLLASRGAFSIEGIHPSTFPFIVATAF
                     NHAPSQIIAVTADYHAMNQFIACLSAFTSEEHIFPYPPLEVLPYEMVNPSSAIERDRI
                     TALYQLLSGNPVIVVATVESLLRAIPHKEFFLKKGITIHKGEEYPFEVILETLASYGY
                     ERETRVDRFGQFSVKGGIIDIFLPNCTNPVRCDFFGDTCESIRYFDPVTQVSLTETDA
                     ITIYPRRELLLSPSQKEQLKKALQPVGDSSLQKQLDSMEKQSIPGIYDLFPIIIPGTT
                     ILSIATQPTVFLCDPSQLKAKLAELERTYHQLYMHKKDMAVFPHDSQILLGDSYPEIE
                     KQAVRLQPFIADSSLVWQLKSIPSFQGRIADVRSECSKRIDEGWHIIVSTSFEGQARR
                     LYDLFGEFHPEDTFDAFNPDKQFTIVISNLHEGFEISAIKTLIITDHEIFGKSYRKKK
                     HFKQKYSRPIDTFLELSPGDYVVHINHGIGVFKAIERMSAGGVERDFIVIEYADGDKL
                     YVSLDQINMVQRYVGLDGRQPRIDALGKKSAWNRIKQRVQESVEEIAHDLLKIYSQRK
                     ALKGYQFPPDTIWQEEFESKFEYEETPDQITAIEDVKDDMEKSQPMDRLVCGDVGFGK
                     TEVAIRAAFKAVMAGKQVAILVPTTVLAMQHYNTFIKRFADYPVTIDMLSRFKTQQQI
                     KSIKENIKAGTVDIVIGTHALLAKDVSFKNLGLVVIDEEQHFGVKHKEHLKKLRLLVD
                     VITLSATPIPRTLHMALAGIRDLTIIATPPESRQSIETLVIEDNPDIVRMAIQNEIQR
                     KGQVFFVHNRISTIYEQAQYLEKLVPEATFCVAHGRMHEHELEEVMVDFINGRYDVLV
                     STSIIESGLDMPNVNTIIINR"
     gene            complement(2793..3518)
                     /locus_tag="PLJ75_03580"
     CDS             complement(2793..3518)
                     /locus_tag="PLJ75_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04329.1"
                     /translation="MRYLSILIILFLTCTGAFAQINTAAQPLQYPGATISTQTPYFIW
                     CDIYNTAAKPFVVKLTLTDSRGQSNEFTLTPLTVDSIYCVATIPVVLTPDTYTYTIHL
                     LSNNKPVNSRYYHHKRYPVKGSFTIDVSRHNAVDTLNANELVYYLSQSRSNMLHNGYN
                     AMFFSASGIISTCAGVAVYYWTNFGIVTTIISAITITSGVIGISAGIYYGMQYYHNEA
                     TINKVVTHDTVPQPHLAPDAGKQ"
     gene            3605..4306
                     /locus_tag="PLJ75_03585"
     CDS             3605..4306
                     /locus_tag="PLJ75_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04330.1"
                     /translation="MKNLVLVVLLLLSGQTDTDELQKLFHNFNVQSIDASIVQVIAET
                     GKETQRYEGRYRAYNDCMRIDYSKPFRQIVIVNGGKLLWYYPETKELWTMSQGNVPAV
                     HPYAQRDILNERIVVKSKKKVWYGFFNSGYRYTLFDSKTGVTLELTIDKDKGYPVKKI
                     TYTAQSIEVMRELYSDYTLIQGIWFPATVEVIARTHTGTTRNVTRYYNIILNGNVSTD
                     IFTLQLPKGVRAKTM"
     gene            4314..5690
                     /locus_tag="PLJ75_03590"
     CDS             4314..5690
                     /locus_tag="PLJ75_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04331.1"
                     /translation="MRQCSGVLFFLFFFLLFAADCMATIAVEGLTVNVDIQDIINQGV
                     NNQKEAEYWAIVITNRLHSQGYTTSYVAHYELRDDVCTLYIVESHIADIQVHTDAYIQ
                     DALTQDVTALLGSVYNKHTVYNLLAQLKQKYMLRSINVNVVNYNNGSDVSLIIEAKPE
                     HFIYSFTIAMLPIYGIKPAITVTMPMQDAYCSVKGQMAFDDQRITLKQGGFDYMNYRS
                     KAAWHTGIDAQEEYGVWERYSRSFTLTQCRAYLGLGTIMEAGMYDVSAFIYGVAAYYS
                     MSELENDVSHDAGAELRITATDQRHSIVYEKSMVTLSFIAAYANEAFITSRIAGSVPI
                     QITSRFYCIPNIYSFHTTSSERIYAEYVFDSYLLGYSDRYTSTHSRHIVGMQLVYEAL
                     YELLYCKIFGSAGIYKDEYAAWDNTASYGFAVDIVYGRIVATVGCAWNVEEHFDTCYF
                     FTGVKARW"
     gene            5877..6302
                     /locus_tag="PLJ75_03595"
     CDS             5877..6302
                     /locus_tag="PLJ75_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPD04332.1"
                     /translation="MDTECGMVKEVRGNLVCVAADPASLCATCSSQCMMRQKGRDIWI
                     QNTLDAKVGDRVYFVFPPGAVVLSSGVLYGIPIVLLIAGIIIGALYPLPFFNDRELSA
                     IVMGIIFLALSFVFIQIVSQYIAAKNMVNPVMVKVEKAV"
     gene            6665..7153
                     /gene="greA"
                     /locus_tag="PLJ75_03600"
     CDS             6665..7153
                     /gene="greA"
                     /locus_tag="PLJ75_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPD04333.1"
                     /translation="MAVNIADKKKQVKEEIEKLQHELKIELPKRIAEARSKGDLKENA
                     EYHAARERQSFVQAQISYLSKQLSLIDSIDISTIEQGKVDFGSVVTVRNLDNDEVARF
                     TIVTPDEVDASEGKISLSSPIGRALHKKTVGDEVSVIIPAGTKRFRIEKLVTIHGEEL
                     SL"
     gene            7173..8396
                     /locus_tag="PLJ75_03605"
     CDS             7173..8396
                     /locus_tag="PLJ75_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPD04334.1"
                     /translation="MATIKEFTALRPGKDVVTKVASLPYDVVTAQQAKKIAQNNPCSF
                     FYVTMPEIDLPLSTNPYSDEVYKLGKKKLSKFITDGIMVQDDKPYLYLYTLQIDSHKQ
                     TGIVAVVSIDDYINGIVKKHELTRKAKEMDRMNHINIVGAQTGLVYLFYKDSNDLRNI
                     MHHALSSPVLYDFVADDGVRHIIQRIEDGDYAQLVKQAFDEQILYIADGHHRAASAVR
                     VGLQRREQGYTGSEPFNYFIAAIFPHSELNILPYNRVVKDLYNHDFETFMRLLSDSFV
                     VQKTDNGIAKNRHEIRMYYDHQWYTLTFIKKLPLDAVDALDVSILQNTILSPILGIKD
                     PREDMRIDFVGGTNAIATMVELVDSGSYVIGFSLFPTSIDELMTISDRGEIMPPKSTW
                     FEPKLRDGLLVYCID"
     gene            complement(8402..8474)
                     /locus_tag="PLJ75_03610"
     tRNA            complement(8402..8474)
                     /locus_tag="PLJ75_03610"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8439..8441),aa:Ala,seq:ggc)
     gene            8757..9038
                     /locus_tag="PLJ75_03615"
     CDS             8757..9038
                     /locus_tag="PLJ75_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04335.1"
                     /translation="MAKEIDELRKIVQTYENVLKLNEQELANAYQIISMYENIVEYSR
                     TELKLATETAQARESASQLSRMELMQAFEKIQKLEEDNKKLRAKKENAS"
     gene            9074..10426
                     /locus_tag="PLJ75_03620"
     CDS             9074..10426
                     /locus_tag="PLJ75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04336.1"
                     /translation="MLITYAIIFLFIIILVIFVLYFANIYIFPRKLEEIQQLIESGQT
                     KIAIRKLNDIIEKDDRNHYAHYLLALAYEKENNIQYAILEYRQVLKIGRYDEKVNEVN
                     IRNHLASIYKARNAVEEAKKEYLLLTKLDPANFEPFYELGIIYFNAGQIDKAIPYFKK
                     SIANNNKHGASFYYLGQAYYRMQNYPDAKQAFIEAIKVDPHDYKAHYFLGLVLRQLGD
                     YEWAIKEFETAQKSDDIKVKCFLAKGTCYLEREQLPKAVIEFERGLKFAKRGSDTELN
                     LRYFLAEAQEKMRDLHSAISNWEKIVAVNPTFRDVQAKLKSYAEFRQDDRIKDFMIAG
                     LAQFEHTCRKISESMGLTVMDVDIISDTEVEIIATDTEGKWRNTRRTNKIVRILRTTD
                     VVNDKLLRKLHEKLKEKNATRIIIITAGDFTQSAVDFSNTRPIELYGKNDLLRLLKQI
                     "
     gene            10431..12239
                     /locus_tag="PLJ75_03625"
     CDS             10431..12239
                     /locus_tag="PLJ75_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD04337.1"
                     /translation="MVNKAILLLNQVPIFKHLMADELTALSLLAKPHTVKKGSTFHCN
                     AQSIYIVEKGVFGVQKSSESIYLSRTSYFGQLPFCEKSTGVVRALSDASVLEFANTDL
                     YQFICNNFKALRGYCRILDHYRIPLSQTATTLLKNKACVYTIFSTDKHCGKTTLAISV
                     ATACSYYKRTILLDASYEGISVFDMCGKPLIPPLSQKNPDDVTGYKFIQQRIEKISET
                     MDIANIAYGSKVKINPDIINPLLFYLSFEYSYIVIDCSNSDIHLRDVIMKNSDSIINV
                     INEKSAINHQVVNDTLLTQGQQVYNVVNKHFVKIQKIDQPSYVFKTIPVETNETIFQA
                     VLKNKMYNDELFKNIVSDKRAMVLSHGMYETLYYSGVLESLREEKLFDVIYANGIPYA
                     VLMCYLATQTTEDYKKLLFDYFSDDSLHTFFDIAFPDKYLFKNKPLHNFASAISHIKR
                     IEWNAILPYTQLMHNNTQRFFSTGDAEKIISANLAFSPLFEGISIHGDAYFSAFPLYP
                     LIPESLLRTHINYIGYIAIANPYNPISVKSKIPGLYRKIVDFLPDCYLYACDNTFCCN
                     RLISLTLPDVTSTHDLIKQSLKNTDMFISMIKKMIK"
     gene            12280..12726
                     /locus_tag="PLJ75_03630"
     CDS             12280..12726
                     /locus_tag="PLJ75_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04338.1"
                     /translation="MRSKATNILQTGVLLTGIIYIIIGLLYGFSPIYFANIFGIEVNP
                     DWYNLIKYDTFIAPLYHFSRIFALLLAIVGISMLLPLFDPLKYRGMIYYHSILFPLVS
                     VPSLLINGIAYKHLPLTICGCLLLIIFLFVGFGLIITKKYAKMGLE"
     gene            12843..13181
                     /locus_tag="PLJ75_03635"
     CDS             12843..13181
                     /locus_tag="PLJ75_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04339.1"
                     /translation="MNLKTKKVGNIVVVYLQGRLDVHLSADIEKEINKLIKDDPASHL
                     LLNLAGVEYMSSSGLRIFVSTMRILKESNRKLKICSMNNAVKKIFEVVELMDMFEIYE
                     TEEEAIASFK"
     gene            13271..15451
                     /locus_tag="PLJ75_03640"
     CDS             13271..15451
                     /locus_tag="PLJ75_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04340.1"
                     /translation="MAIPLELSEGIKNFEIYVKHNYFSWPYWIAHYTNRENVRIQSLP
                     FILYNNHCRTWYTINSFLNSGAIRIDPCGMIHPDWAKWSVECMVYSDGAYIKFHDDTC
                     IHQQNERGVVKTSYQHHLFRFLQSVYIAHTNVDEAIVINKINIKKKNQSYFIIAIRPY
                     TTTTLSGINSIELLKNNLVKINDDHACSLIEKPDYILVGNEIIGDIDILQKDDKYRIS
                     SSSGMATMAFAYELKNTDHEYGIRIATGKNTKLSVSTLSVKSALNDYTSYVDSIMKQG
                     IAISIGRHTINEWTDFCKLSTLVHASTMASIKTTQLQRLKQYYYISSTLNRMGYLKQT
                     ELAIDTIKKNIKITDDYEGVLSSSFFCLIVGDCYRTIKDSSFVQNNYSLCKQYAYSIT
                     EFFKKIGDITKENNFKDNPQIAINAIINIAYIISALENVAFMSRSLGLFGEETKIFSD
                     IKRLQNVILKSIFTINSETNGEEEKLIEWTPKYSKPIRKQKIIVKDSLEFSHFVNVND
                     NRIISYSPLLYPYMKDIMQPYIVKNIINAIIHAGKGLPLYNTIISAVDIVNSLSLANA
                     MIYAHDKRAIDSINAIINLCNKQKFLPDFLDPVTGHAVVNDPVSTIAVSLLFQAIRNL
                     CFIDHPERLEIFPVPWEEWFNDCQMNIKNAHSRFGIISFTATSTSNEFVIQFTDIPRF
                     IPHDILVNLPFKAKIKKSDDFIVKYQTEKSWCINGWPSEIRFKR"
     gene            15521..17065
                     /locus_tag="PLJ75_03645"
     CDS             15521..17065
                     /locus_tag="PLJ75_03645"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04341.1"
                     /translation="MISYLVISLLIISGFVYYLVYIRPKFNPLNKAEQFLKTNQLHDA
                     IIEYKKVIDEYPDNFVVHHRLAELYLTMGEIDQAVIHLEKILEIGKFNYEVDQLSVLK
                     KLAKSYYVRDEVEKAFKMYMDILKNFPADIDSLYHVSFILLSQEEFDIAQRYFERLLK
                     LKNDSYEILFGAGICNYQNMRIQEAIDNFRLALQLKPQSEIAQIALAFAFNRKRDYRQ
                     ALTYITKVAESAQDPDVQYLAKRFQAFLYMRLKRHDEGIKLFQDLLEIARKNANDDAE
                     ALALHDLGYALLKNEQSSLAYDVWNELYRKDRNYKNIQFLVTQLRKEMEADKFVKEES
                     ASITETITAWMEEAFPQNLLWDMCGLKGSKRYNLKDVVVTTRITFDDGKLSSTSDEII
                     QKYIALDTENFRIISNRLVSKVGFKVDQILQTYREADGVDFLAVNPETKDKVLIWVRR
                     WTKTNVGEITLRNFAQAINDMKVKQGILITTADLTDIAKETLKQLSKISVIYPDEIEK
                     LLRGLL"
     gene            17066..17995
                     /locus_tag="PLJ75_03650"
     CDS             17066..17995
                     /locus_tag="PLJ75_03650"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HPD04342.1"
                     /translation="MPFDNFTSTMIPLQSINYINDYYKISKPLSPELIKSIKQNGVLE
                     PVILIEGNGCYIPVCGHNRMLASIQTELANVPAKIINGLSEDDFMAIITLKLYNNLMG
                     TIGKLKSLFILKEYFKINKKQLIAIAHELSVPDALIETTSLKKIIGLPSSLMNYIDSK
                     NIPFKFIMPVLRLSDESIQLVAAIINACQLRVNYFRDIVVMIEDICRNQHYESVLKKI
                     TVLINKENRTDDEIYRNIYLLRYPQYSEYVEKINYLKNKFLSYGLKIEVPEYLEGNEV
                     NIIIQVKKNEVVHPLLQQDKEIRALIQSIIELL"
     gene            complement(18006..18983)
                     /locus_tag="PLJ75_03655"
     CDS             complement(18006..18983)
                     /locus_tag="PLJ75_03655"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPD04343.1"
                     /translation="MDYFANLEKYLNAHDHFIISTHESPDPDGLGAEIGFKEILLHLG
                     KHAIIINSDPTPTKFNFIDPDNEIIIFDEKMIIDNISNYTIFVLDTNDFNNIGKAYHY
                     LNNIVKDVFIIDHHEGANNKLETNYIKVEASSTSEIICTIMERYNIKPSFKAAQALYA
                     GIVFDTGCFKYPKTSSHTFTIAAQLISSGIKPYEVYQWIYENNTLEVFRLRALMLSSM
                     EVHFGGKLILMELTPEMIKATNAPFSEGEVNINLPLTINGVVASVVVKQDVDGPVKVS
                     MRTKGDLDVAAIAIANGGGGHKNAAGFKSPYNFETTKQKILQEMSAFFK"
     gene            complement(18983..19888)
                     /gene="glyQ"
                     /locus_tag="PLJ75_03660"
     CDS             complement(18983..19888)
                     /gene="glyQ"
                     /locus_tag="PLJ75_03660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HPD04344.1"
                     /translation="MYFQDLIASCNSFWAKQGCIIAQGYDMEVGAGTFNPATFLRVLG
                     PEPWNVAYVEPSRRPTDGRYGDNPNRLQHYYQYQVIMKPSPLDIQDLYLDSLRNLGIK
                     LEEHDVRFVEDDWESPTLGASGLGWEVWLDGMEITQFTYFQQCGSIELDPIPVEITYG
                     LERICMYIQGVESVFDIQWNDTLTYRDVHHRSEFEFSHYNFSIADTALLFQLFDLYEK
                     ECLKILDYEPVQVILPAYDYVLKCSHVFNLLDARGAISVTERVGYITRVRNIARRCAE
                     RYVAIRESMGFPLCKYAKYASPEKA"
     gene            complement(19976..21496)
                     /locus_tag="PLJ75_03665"
     CDS             complement(19976..21496)
                     /locus_tag="PLJ75_03665"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPD04345.1"
                     /translation="MEGTQQTSHYFCKPVDYKEALTRGEDVYLQFSLLTPEVEACLIR
                     VLSIYLSQLDLLYLKDMFINILKELVNNAIKANAKRLYFKRKNLDINKKEEYRNGMET
                     FKEDVFVGNPEFLEELGKSNFIVRVFFSITGDIHKIHVINNVPIIPEEMNKINARIKK
                     AYTYHGISDAFDDILDDSEGAGLGLIITLILMKNAGFPPEAFSMSTNGKITSATINID
                     ESFRNMDLKVKMAQEIINEVESLPSFPQNILEIQRLCANPESTIKAIADSIKRDPGLV
                     TAILKLANSAGYITSKRVETIEEAVKIIGTKGINTLLVASGVQKIIDSRYKKFETIWN
                     NSYKTAFYAQWIAMKYRKERLSEFAYLASLLSDIGLMVLLSIKPDEIQKIREIVGLKQ
                     LDNISILEEISLGVSHSTLGSLICQKWKFNDALVKAIEFHHRPHLAPSQYRDLIYIVY
                     LAYVFTSIESKNFRFEIADEDCLDYFKLNNKTNFDELHQLIKAKFEEYSQNNKITN"
     gene            21972..23072
                     /locus_tag="PLJ75_03670"
     CDS             21972..23072
                     /locus_tag="PLJ75_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD04346.1"
                     /translation="MSVVYFTDLHMSSKENIVSKIKKLFYKLSANEIINEKDFVAIKT
                     HFGEYGNIAYTPPVIIHTFVDCVKDLKANPFVTDSTTLYRGSRSNAVDHLKNAAMNGF
                     SLVSCGAPVIIADGLRGNDFKELPATGKWYSTIKVASAIHDADSMVVVSHVKGHELYG
                     FGGAIKNIAMGCAPPSMKQSLHSDLKPKVKQTLCTSCGACIKRCPAFAISFDANKKAF
                     INSDKCFGCGECTVICVYNAIPVLWKTDHTTLYERTAEYVNAFMKHKENKVLFFNFIM
                     NVSPECDCCYWNDIPIVPNIGIVASTDPIAVDKASIDLINKATPMPDSRIAHKDSSKD
                     NIEALYPVKWNCLFEYATSIGAGNMEYELVTI"
     gene            23106..23546
                     /locus_tag="PLJ75_03675"
     CDS             23106..23546
                     /locus_tag="PLJ75_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04347.1"
                     /translation="MKSPHDFPAINPKRPDGIKPNGIPYKVMVIDDKEFHRKQIIQIL
                     ESEGYEIIAAASNGAEALKLYESLAADLDLITTDLDMPKLDGYALMYELSHKKPKAKI
                     VFISEETTKGVLSDLLKMGAADFILKPIQRGTILERIKAVLQKK"
     gene            23653..24729
                     /locus_tag="PLJ75_03680"
     CDS             23653..24729
                     /locus_tag="PLJ75_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04348.1"
                     /translation="MEENDKAYMHLSEQTRKINIAMHYTHGDMERARQMVSGNYNDLY
                     AIKVRFASSTLFGAFLLFFNTIYNSISGLSLIVSPSYTLLNIDPALDWILFEKEIAKQ
                     MQKDEYDHVLAKTFSDKMIKNFSTILQNELSRNIEIKNDIAIDNLFRKLIQETFALQR
                     VDSLVVYQSTSSLTMELNSITIPKLESVKVEGEQTQESEVIKADTPSGEYTPLVGVDG
                     VKLIIKATLILSPIKGKHISQLSAGDRIMISIIDSKPVAVKVAKALQAYQDEQFKPIP
                     ARITHVQCIPQKGYKIYGLIAKGIVVDITEEEENIKVALDPTYAATVQAAEEEAKPNY
                     IIIAIVAVTFLIVIGILYLLLNAN"
     gene            complement(24722..25489)
                     /gene="hisF"
                     /locus_tag="PLJ75_03685"
     CDS             complement(24722..25489)
                     /gene="hisF"
                     /locus_tag="PLJ75_03685"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPD04349.1"
                     /translation="MLAKRIIPCLDVKDGRVVKGVNFVNLSDAGDPVNNGMFYDAEGA
                     DELVFLDITASSDQRAIILKMVKDVAETVTIPFTVGGGIRTIDDVRMILENGADKVSI
                     NTQAVLMPQLITQCAMRFGSQCIVVAIDAKQNSAGWNVYLHGGKTKTDIDALQWAKQA
                     EALGAGEILLTSMDRDGTKAGYDLKLTQSIADIVHIPVIASGGAGTLEHLYEGFVYGK
                     ADAVLAASIFHYREYSIKQAKDFLASHGIPIRPVDFS"
     gene            25603..26670
                     /locus_tag="PLJ75_03690"
     CDS             25603..26670
                     /locus_tag="PLJ75_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPD04350.1"
                     /translation="MSKKIEKHNTFHDFSSRYVPLINQYIDSFYNNKELSAKNHELKI
                     AYRLLQEYCTREGKRIRPLLLLLAFAGYGGLVKKINAMIPVASSLECMHAFLLIQDDI
                     IDGAVVRRGKPAFHILCDSSYYQYNANIGTWVAIVLADVVMSNALEMIATLESSRIKY
                     FLNEFAVMYERTALGQLLDIIWSHPETISDDGIAEMIATMKTAHYTVSGPLKLGYILA
                     GGNDSNELQRLTEMGYSLGFAFQMRDDIIGVFGDEKDMGKSASSDIAEGKYTVLVQQA
                     WNKLNEKDRTHFLDLLRKDEKSSEEIDTIKHYITKSGAVTVLHAIIDEHLQKVHAILP
                     QLQCNDFMKQCMLQFIEGLFQ"
     gene            26925..27608
                     /locus_tag="PLJ75_03695"
     CDS             26925..27608
                     /locus_tag="PLJ75_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04351.1"
                     /translation="MAMVAKKAHKETFERISPEKRDRIITIATKEFAKNGFKGANINI
                     IAKKAGISIGSMYNYFESKEDLLLTVINEAYKLLENVLGSVSLENKNFYEKIEELVCA
                     AQKFARQYPEIHQVYLDITSEGLSHLTPRLSRTIEYITIKFYRHELEQAKIQGIVRED
                     ADVNVASLCIDNILVLTQFSYTSEYFKERLKLFIGEDAFDDDERIVKGVVDFIKNALM
                     PRMNIHNNK"
     gene            27644..28243
                     /locus_tag="PLJ75_03700"
     CDS             27644..28243
                     /locus_tag="PLJ75_03700"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04352.1"
                     /translation="MKRKNVNVIELRRNQLTRAAYKVVSRKGYYNFTVRDIAKEAGLS
                     TGLVHYYFKNKDDLLVSVLRVMNENLSSYLAKALEKIDDPEEKIIIFMDEAFHLVERE
                     KEYFHVLIDFWTQINHNERMRKANIKLYQSYRAECAKIIQEGIDKNVFKDVDVQYTAT
                     MIVAFVQGLIIQYIIDNEAFDYTEYTKKIKLQIVDNLMK"
     gene            28339..29280
                     /locus_tag="PLJ75_03705"
     CDS             28339..29280
                     /locus_tag="PLJ75_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HPD04353.1"
                     /translation="MLKDISENGNYQKHLENIVKTLCQPQSYKKVYHRINHDVPMPSI
                     QQLEEIVEECKMVLFPGYFGNSEITPHNMQYYIGSAIDGIARKLTEQIKRGYCFSCSE
                     NETIDCIECEHRSRKIMFQFLDMLPEIRRMLAMDVQAAYEGDPAAKSHGETIFCYPSI
                     TAMIHYRIAHALYILDVPLIPRIITEMAHSQTGIDIHPGATISENFFIDHGTGVVIGE
                     TTIIGNNVRIYQGVTLGAKSFPLDENGNPMKGIPRHPIVEDNVIIYSGATILGRVTIG
                     KGSVIGGNVWLTESVPPKTHILQQKPSEMMFEHGGGI"
     gene            complement(29277..30245)
                     /locus_tag="PLJ75_03710"
     CDS             complement(29277..30245)
                     /locus_tag="PLJ75_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HPD04354.1"
                     /translation="MKTKEFISLKWSLYEKNPIIKYPFPSPIIADPSFITPDESKDGK
                     WHLFAHSLLGIHHYISMDGITYTKLKKQTLRRAMRPFLLYDNGMYYLYYEKTKPFILW
                     YSWLPHQWYSYIAMRSSKDCYTWSNEKIILKPSLPWHHNNLGYSISNPCVVKTKSTYY
                     LYYSAALAYIMDCGFNEPLHIGLAKAQSPEGPFECCSEPILSPSQYNRWCNLGCGSFK
                     VLAFDDGFIGLQNGIYWDYTINHSGSAILVYSSKDGKTFKTLLKDPLLKPDNINGSFM
                     KSHVYAFDAKPVDKEQWFLYFNARNDWHWSKGREHIGLLIGKKNKH"
     gene            30430..32451
                     /locus_tag="PLJ75_03715"
     CDS             30430..32451
                     /locus_tag="PLJ75_03715"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04355.1"
                     /translation="MVLLTRFFWDNNDMYSINVKAIKILMLNCIIVLISCSAHSPGVF
                     QSLEGTWKLATKNIEYDYSNKRLPEYVEINIPCSIKVPHTFENNNMQLPVKGYVWIQK
                     EIFIESLPANDLALQFGEIMNADVAYFNGKQIGASGRFPPDFKSAWGKFRHYTIPRDI
                     INKGANVIALKVYYDSEFWITPPVRLIDNVAGSKIASLLDYLRIQLMENIFILLILLS
                     FFFLMMFYFQREYMEYLLFGIACFTIAMPQALHFLENLYPDIGLTSDTILKLTQPGLI
                     LFPAFLNLFYRYHIGKQKKWLTIVYIAVPIVFSTIMVLQQTRAGILLYRNLSIIVSIV
                     YMIDLFILSGWQVLHKKKAGALIFIGLVPMVVLGIHDVLAFGLHIIPSYIVLYVYGLP
                     FLIFIIAIYLVNIFVKALKEAKDLNIQLQKLLLDAKRLAYLEKELEIAKAIQSQLLPS
                     KIPNIEGIDIAIKFQPAMKIGGDIYTIFQIKDGVVFFVADVVGHGIPAALISAMVNVL
                     LKMFQMLAIDPALLLRSLNVNICTMLPQTFVTAVCVYCNRSHNFMRIARAGHPPMIKC
                     SNDGNISMYMPSGRALGIQTQTRFKTVELTMEKGDRLIIYTDGLVEASLPGNSKDIYG
                     EQRLKNMIQQTINLPLDKAIEVVYNDVISYVGSPQYLTDDITVIMIEIL"
     gene            33206..33568
                     /locus_tag="PLJ75_03720"
     CDS             33206..33568
                     /locus_tag="PLJ75_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04356.1"
                     /translation="MKKIVIGIFLLLLTNSYAQSFLYELSENIDNYKNKNITMIMKLK
                     YYDTLFQVITFYDAKNHDISFDCIDVMKHPWFLQQKNNLHPGMEYNVTFTVKGLGNMK
                     EIIAQLQYFEPVILQKIP"
     gene            33891..34391
                     /locus_tag="PLJ75_03725"
     CDS             33891..34391
                     /locus_tag="PLJ75_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD04357.1"
                     /translation="MKNVLAVMSVFAFAFMVACSSTPAVQQPAGDPNVALVNAQNKQL
                     ERLPVTGFAYKSSVVPKQDWDKWVEAAAPVVKEVINKLPEGYVLQVTGHTDASGPEEA
                     VGNKPGNLKISTDRAKAVYNALKAKGIDSPRMTYKGVGSSELLPGVDPRDGANRRVTF
                     KVVPKK"
     gene            34493..35425
                     /gene="rsgA"
                     /locus_tag="PLJ75_03730"
     CDS             34493..35425
                     /gene="rsgA"
                     /locus_tag="PLJ75_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPD04358.1"
                     /translation="MKGVVSKVMGLYCTVYCDAIYINCVMRGKLRQLIEAGSKKFSNI
                     IAVGDEVVITKLDDGSGVIEEILPRRNYFSRKEKGKHKKIDMIAANCDCVVAMQSFAL
                     PRLNLRFVDRIAVRCVEQNIPLVVCINKCDLAKKKDYEYVTNYYKSTDVTVIISSVNT
                     SYNIHALQDILHQKTSVIVGYSGVGKTSILNAMYPQLSLVVGEVSGSTGKGKHTTTNV
                     SMIIIDETTRIIDTPGLREFGIMDIEPHVLSKYFYEFDNYAPKCSFKPCTHDHEPDCE
                     IKRLVQKGIICEERYISYRNILYSLKDYYATMYS"
     gene            35422..36174
                     /locus_tag="PLJ75_03735"
     CDS             35422..36174
                     /locus_tag="PLJ75_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04359.1"
                     /translation="MIALEHVQLLFNNHYVLQDVNLFVQQGSTYVILGKNGSGKTVLL
                     KTIAGLFPQFSGTIAINGISLHEYTQLPINEKIHKNITMGYVFQKGGLFDSMTVYDNV
                     AFGLQRRASTEDEIKNKVLSALEWVGLAGSENKLPSQLSGGMQKRVGLARALCLSPAI
                     ILYDDPTAGLDPVLTDSISELIVATQNKYAMTSILVTQDLAVAEKTAHRIGLLHNGMI
                     IVDCSADEFFSKTNPYAKQFIDGDSEGPIEII"
     gene            36420..37457
                     /locus_tag="PLJ75_03740"
     CDS             36420..37457
                     /locus_tag="PLJ75_03740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPD04360.1"
                     /translation="MQTERTKSDLDWKNLPFGYTKTDYNIRYYYQDGKWSAGELSSEE
                     TIPIHIAAPGLHYGQQAFEGLKVFETVDKRIVAFRPEENARRMKVSCERICIPELPMG
                     LFMEAVEKVVATNTKYIPPYGTGASLYVRPVIYGAGARVGLGPASEYVFIVFATPVGP
                     YYKGGFKPVKALVVEQFDRAAPNGVGDCKVGGNYAAGLRGDEYAKKLGFPVPLYLDPK
                     EKKYVDEFGTSNFIAIKGNTYLTPESSSILPSITNKSLMDIAALLGMNVEKRPIAIDE
                     VEHFDEVGAVGTAAVVSPVCSIHYRDKIFTFCDEDKAGPVLTRLYEYLTKLQTGEIKD
                     TFGWLHEIVVK"
     gene            37491..37916
                     /locus_tag="PLJ75_03745"
     CDS             37491..37916
                     /locus_tag="PLJ75_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPD04361.1"
                     /translation="MNNQDICWIQRFKHLMQAFLQLEKAIHLANQRSLTELEEQGLIQ
                     SFEYTHELAWNVFKDFLEEQGIQNLYGSKDTTREAFRRGLIENGEIWMDMIKSRNLTS
                     HTYHSELAQEIIKRIKESYFEELKRFIEKIKLLYNNQIS"
     gene            37913..38221
                     /locus_tag="PLJ75_03750"
     CDS             37913..38221
                     /locus_tag="PLJ75_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04362.1"
                     /translation="MNFGLSNTIIEQICSVFSKHPVVEKVIIYGSRAKGNYKNGSDID
                     IALIGGKDLTVDVMFIIIEEIDDLFLPYTFDISLLRYINDHDVLDHIKRVGKVFYKKK
                     "
     gene            38322..>39167
                     /locus_tag="PLJ75_03755"
     CDS             38322..>39167
                     /locus_tag="PLJ75_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPD04363.1"
                     /translation="MLSKQQYALLLDTFKSAQNAKNSDAFLSDIVDLLYNELKPISVS
                     VYTLVGDERLVLHHEAGKNFDELVVNIYNFSLIVRKAIKKKSVLFLPTSKPFQNDEIN
                     ISVAIIPLLHSDIVVGMICLVFPLEELQHIQNSTPFFTLIGKIAGTIRYYQTGLKVAK
                     ADTAEEEFEQKITSLRVLAQGVAHEFNNILAVIKGYAELLLMNEAVDESVKEAVAIID
                     KQTIRGSSLIDRLSVFVTGKEISFEYCSINDLIKEIVDVQRFTLEKESIECKLKLGKV
                     PEVFVD"
     assembly_gap    39168..39267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <39268..40081
                     /locus_tag="PLJ75_03760"
     CDS             <39268..40081
                     /locus_tag="PLJ75_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04364.1"
                     /translation="ESIECKLKLGKVPEVFVDPRQIKEVLLNLITHARSAIKPNKKGT
                     VTIETSFNSDAVMLKFVNDGVPGCWENGDPDKIKATNGVDSVGNGISLAIAFGIMKSH
                     GGDMTLCHDPAKCNEVTIYFKKFNPSFADAEKKEEKEMLYLGEVRILIVDDEEPIRQF
                     LSSAFERMGYQVLTTDNGQEAIDICTFEDIDIVFLDYLMPGYKGDAVFKSIHDVSPAT
                     DVVFITGVDSIPDIDKLLMMGLEGVLRKPFKIDTIIKLTNEIMARRYNTLQE"
     gene            complement(40090..40287)
                     /locus_tag="PLJ75_03765"
     CDS             complement(40090..40287)
                     /locus_tag="PLJ75_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04365.1"
                     /translation="MAKPLLGEILVEDAVITKEQLNEALAIQKRDGGLIGIILMNLGY
                     INESTLVKYLAVQAERVIKSE"
     gene            complement(40350..41834)
                     /gene="glpK"
                     /locus_tag="PLJ75_03770"
     CDS             complement(40350..41834)
                     /gene="glpK"
                     /locus_tag="PLJ75_03770"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002741576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPD04366.1"
                     /translation="MGKKFLIAIDLGTTGNRVFCFDEHGLPISSSYAEFTQYFPKPGW
                     VEHNAMEIWDSVAQLIPDAIAKGNLNPNDAIAIGITNQRETSVLWNEETGKPIYNAIV
                     WQCRRTTDICNQLKEAGYEELFREKTGLVIDAYFSGTKVKWMLDNVKGARDLAKSGKL
                     LFGTIDTWILWKLTGGKSHKTDYTNASRTLMFNIKNKSWDLELLEILNVPRTILPEVR
                     ESASYFGETQGVPGLPDAITIGGIAGDQQAAMVGQNCVFEGTSKNTYGTGCFMLLNMG
                     NEWILSKHGLVTTLTCDNKGQPVFAFEGSVFIGGAVVQWLRDYMKFIEKSSDAEKVSL
                     SVNKEDEVVFVPAFVGLGAPYWKMDARGAIFGLTRDTTPEQIVRAAVKSTALQSYELL
                     EAMQSDSGKNIHELRVDGGATRDTYLMQYQSDILGIPVLLPEITESTALGAAYLAGIT
                     GKVYNTIDEVAKFNKIAKRYNPSMNETTRQREINLWKNAVRRLL"
     gene            42443..>42620
                     /locus_tag="PLJ75_03775"
     CDS             42443..>42620
                     /locus_tag="PLJ75_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002844980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04367.1"
                     /translation="METNFHNRYLEKIAQKKPVVIAEIGLNHNGNLNEAIELIHRAAD
                     SGADLVKFQIINAEL"
BASE COUNT        13822 a         6905 c         8296 g        13497 t
ORIGIN      
        1 cggttgatga tgatggtgtt tacattgggc atatcaagcc ctgattcaat gatggatgtg
       61 gaaaccagaa catcgtatct gccgttaata aagtctacca tgacctcctc aagctcatgc
      121 tcgtgcatcc tgccatgtgc cacacagaac gttgcctcgg ggaccagctt ttccaggtac
      181 tgtgcctgct catagatggt actgatgcga ttatgaacaa aaaatacctg ccctttgcgt
      241 tgtatctcgt tttgtatggc catgcgaact atatcaggat tgtcttcaat aaccagtgtt
      301 tcgattgact gcctgctttc cggcggtgtt gcaataattg ttaaatcccg tatgccagcc
      361 aatgccatat gcagggtacg cggaatggga gtagccgaaa gcgtaatgac atcaacaagc
      421 agccgcaatt tttttagatg ctccttgtgt ttaaccccaa agtgctgttc ttcgtcaatg
      481 acaacaagcc ctaaattttt gaagcttaca tcctttgcaa gcagagcatg tgttccaata
      541 acaatatcca cagttcctgc tttaatattt tcctttatgg attttatctg ctgttgtgtt
      601 ttaaagcgcg atagcatgtc aatagttacc ggataatcag caaaacgttt gataaaggta
      661 ttatagtgct gcattgctaa aactgttgta ggaacaagta tcgctacctg tttcccggcc
      721 ataactgcct taaatgctgc tcgtattgcc acttctgttt ttccaaatcc aacgtcaccg
      781 caaaccaatc tgtccatagg ctgtgatttt tccatgtcat ccttaacatc ttcaatggcg
      841 gttatctgat ccggtgtttc ttcatattca aactttgatt caaactcctc ctgccatata
      901 gtatcaggcg gaaattgata gcccttcagc gcttttcgct gcgaataaat ttttagcagg
      961 tcatgtgcta tctcctctac tgattcctgc acacgctgct ttattctgtt ccatgctgat
     1021 tttttcccca gtgcatcaat gcgcggttgc ctgccatcaa gccccacgta gcgctgaacc
     1081 atattgatct gatcaagcga tacatagagc ttatcgccat ctgcatattc aataacaata
     1141 aaatcacgtt caacgccacc tgcgctcatg cgttcaattg ccttaaaaac acctatgcca
     1201 tggttaatat gaacaacgta atctcccggc gatagttcta aaaatgtatc gattggtcgg
     1261 gaatattttt gtttaaaatg ttttttcttc cggtatgatt tcccaaaaat ttcatggtcg
     1321 gtaataatca aggtttttat tgccgatatt tcaaaacctt catgaagatt tgaaatgaca
     1381 atagtaaatt gcttatccgg gttaaatgca tcaaaggtat cttcaggatg aaactcgccg
     1441 aagaggtcgt ataatcttct tgcctgtcct tcaaacgatg tgctgacaat gatgtgccat
     1501 ccttcatcaa tgcgctttga acattcactg cgaacatcgg caatgcgtcc ctgaaaggat
     1561 ggtatgcttt ttaactgcca taccagggat gagtcagcta taaacggttg aagacgtacc
     1621 gcctgctttt caatttccgg gtaactatcg cccagcaaaa tttgtgaatc atgggggaac
     1681 actgccatat cctttttgtg catgtagagc tgatggtatg ttcgttccaa ttctgcaagt
     1741 tttgctttga gctgtgatgg gtcacacaaa aaaacggttg gttgtgttgc aattgacagt
     1801 atggtggtgc ccggaataat gatagggaac aggtcgtaaa tacccggtat ggattgtttt
     1861 tccatgctat caagttgctt ttgaagggaa ctgtcgccca caggttgtaa ggcttttttt
     1921 aattgttctt tttgtgatgg ggacaacaaa agttcacggc gcggataaat ggtaattgca
     1981 tcagtttccg taagcgaaac ctgcgtcaca gggtcaaagt agcggatgga ttcgcaggta
     2041 tccccaaaaa aatcacagcg caccggattg gtgcaattgg gcaggaatat atcgatgata
     2101 cctcccttta ccgaaaattg cccaaaacgg tcaacacggg tttcacgctc atagccgtag
     2161 ctggcaagcg tttcaaggat aacctcaaag ggatattcct cgcccttatg gatggtgatg
     2221 ccttttttta aaaaaaattc tttatgaggg atggctctga gcagagattc aacagttgca
     2281 acaacaatga cgggattacc cgataaaagc tgatacagcg ctgtgatgcg gtcgcgctcg
     2341 atagctgatg aaggattaac catctcatag ggtaaaacct caaggggagg atagggaaat
     2401 atatgttctt cgctggtaaa tgctgatagg caggctataa actgattcat tgcgtggtaa
     2461 tctgctgtaa ctgcgattat ctgcgatggt gcatggttaa acgctgttgc aacaataaac
     2521 gggaaggttg aagggtgaat accttctata gaaaaagctc cccgggatgc taatagtggt
     2581 ttgcactcat cagtagaggc atagatttgt gttaattttt tcaggaccat tgcattatta
     2641 caactttata gaggatacta tttagcacca cagtgaatcc gtatcaggct ctgaccccat
     2701 ggttgcgatg ttgattgatg cattgataat tattactata gtgtacagtg tttttgggca
     2761 tttttcaatc tttttatgat gctctgaccc cactattgct tgccagcgtc aggggcaagg
     2821 tgtggctgtg gcaccgtatc atgagtaacg actttgttga ttgtagcctc gttgtgatag
     2881 tactgcatac cgtaatagat tcctgctgat atgcctatta cccctgaggt tatggttatt
     2941 gctgagataa tggttgtaac aatcccaaaa ttggtccaat agtataccgc aacgcctgca
     3001 catgttgaaa tgataccgct ggcagaaaag aacatggcat tgtagccgtt gtgcagcata
     3061 ttagaacgcg actggcttaa ataatacaca agctcatttg cattgagggt atcaacagca
     3121 ttgtgccggg atacatctat ggtaaagctt ccttttacag ggtatcgttt atgatgataa
     3181 tatcggctat tgacgggttt gttgtttgaa agcaggtgta tggtgtatgt gtaggtgtcc
     3241 ggggtcagaa caacaggtat ggtagcaacg caatatatgc tatccactgt caggggagtc
     3301 agggtaaatt cattgctctg acccctgctg tcagtgagtg ttagcttaac tacaaatggt
     3361 ttggcggcgg tgttatagat atcacaccat ataaaatagg gtgtttgggt gctgattgta
     3421 gcaccagggt attgcaatgg ctgcgccgca gtgttgatct gtgcaaatgc accggtgcag
     3481 gttaaaaata aaataatgag tatactgaga tatcgcatag ctttaccttg acaattacat
     3541 gagagtactt tacagtgtca gagcaatgat ggtacaacaa taaaatggtg atgagaataa
     3601 tgcaatgaaa aatcttgtat tggtagtttt attattgctt tcgggacaaa ccgataccga
     3661 tgaacttcaa aaactttttc ataatttcaa tgtacaaagc attgatgcat ccattgtgca
     3721 ggttattgcc gaaacaggaa aagaaactca gcgctatgaa gggcgatacc gggcatataa
     3781 cgattgcatg cgcattgact acagtaagcc ttttcggcag atagttattg tcaatggagg
     3841 gaagctcttg tggtattatc ctgaaactaa agagctatgg acaatgtcac aggggaatgt
     3901 tcctgctgta catccgtatg cgcagcgtga tatcttaaac gaaaggattg ttgttaaaag
     3961 taaaaagaag gtatggtatg gtttttttaa tagcggctat cgctatacat tgtttgattc
     4021 aaaaaccggt gttaccttag aattaaccat tgataaagat aaaggctacc cggtaaaaaa
     4081 gattacctat actgcacagt ctattgaggt tatgagggaa ttgtacagcg attacacctt
     4141 gatacagggt atatggttcc cggctaccgt agaggttata gcacgtacgc ataccgggac
     4201 aacacgcaat gtaacgcgct attataatat tattctcaat ggtaacgtat ccactgatat
     4261 ctttacctta cagcttccaa aaggtgtcag agctaaaacg atgtgaggat agtgtgcgcc
     4321 agtgtagtgg tgttcttttt tttcttttct tttttctttt atttgctgct gattgcatgg
     4381 caacaatagc tgttgagggc ttaacggtta atgttgatat tcaggacatc atcaatcagg
     4441 gtgtcaataa tcaaaaagaa gcggaatact gggcgatagt tattaccaac aggctccaca
     4501 gtcagggata taccacaagt tatgtagcac actatgaact tcgtgatgat gtatgtactc
     4561 tgtatattgt tgagtcgcat attgctgata ttcaggtcca tacggatgcc tatatacagg
     4621 atgctctaac acaggatgta acagcattgc tgggctcagt gtacaacaaa catacagtct
     4681 ataaccttct tgcacagtta aagcaaaaat atatgcttcg gtccatcaat gtcaatgttg
     4741 tcaattacaa caatggcagt gatgtcagcc tcattattga ggccaaacca gagcatttta
     4801 tctattcatt tacaattgca atgctgccca tctatggtat aaagcctgcc atcacggtga
     4861 ccatgcccat gcaggatgcg tattgttcag taaaggggca gatggctttt gatgaccagc
     4921 gtataacctt aaaacagggc ggatttgatt acatgaacta tcgctcaaaa gctgcctggc
     4981 atactggtat tgatgctcag gaggaatatg gcgtgtggga gcgctattca cgcagcttta
     5041 ccctgacaca atgcagggcg tatctgggat tgggtaccat catggaggca ggcatgtatg
     5101 atgtttcagc gtttatctat ggcgtggcag catattacag catgtcagaa cttgagaacg
     5161 atgtatcgca tgatgctgga gcggagttgc gtattacggc aactgatcaa aggcattcta
     5221 ttgtatatga aaaatccatg gttaccctgt cgtttatagc agcgtatgcc aatgaagctt
     5281 ttataacctc ccgtattgct ggctcagtcc ctattcaaat aaccagccgt ttctactgta
     5341 ttccaaacat atatagcttc catacaacat ccagtgaacg gatatatgcc gagtatgtgt
     5401 ttgatagtta cctgttagga tattctgacc gctatacctc aactcattcc cgtcatatag
     5461 tcggaatgca attggtatat gaagctttgt acgagctttt gtattgtaag atatttggtt
     5521 cggcaggcat ctataaggat gaatatgcag catgggataa caccgccagt tatgggtttg
     5581 cagtagatat tgtgtatgga cgtatagttg caacagtggg gtgcgcatgg aatgttgagg
     5641 agcactttga tacatgctat ttttttacag gggtaaaagc acgctggtaa ttttatatgt
     5701 catatctcct gttcatttat ttcatttatt tcatttattc attttttggt ttgtatttaa
     5761 tcagttgttg tattgtcaca tggtagttat tactgagtaa caaataatta ttttacataa
     5821 atgcctgttt ttgtggtata tatcatttat atatcataat taagaaggac cggtggatgg
     5881 atactgaatg tggtatggta aaagaggtgc gaggcaacct tgtttgtgtg gcggctgatc
     5941 ctgcttcgct ttgtgctaca tgttcaagcc agtgtatgat gcgccagaag ggaagggata
     6001 tctggataca aaatactctt gatgcaaagg ttggcgacag ggtatacttt gtttttccac
     6061 caggggcggt tgttctttct tcaggggtgt tgtatggcat acccattgtg cttcttatag
     6121 ctggcattat tataggggcg ctttatccgt taccattttt taacgacagg gagctttcgg
     6181 cgatagttat ggggattatc tttttagcgt tatcatttgt ttttatacag atagtgtcac
     6241 aatacattgc agcaaaaaat atggtaaacc cggtaatggt aaaggttgaa aaggcagtat
     6301 aaaaaaattg tagacatctt ttattaaaga agcaacagta tcactttgac gttatgctgg
     6361 gtgatagtta ctgatacata gagtaactat cgcataactg tagttttgtg ataagttacc
     6421 catggttgtt aagcttacca actacgggat gaaaatgtag agttatcgtt attctgagcg
     6481 atagcgaaga aactccttta taaacgaata gatgcttcgg ggctgtgtcc ctcagaatga
     6541 cggtaatgct ggtatgtcat tctgaatgaa atgaagaagc tcgctgttaa aaggataggt
     6601 tttttggtcg ctaacgcttt ttcataatgg cgcagtacta tttctatttt cggaggaata
     6661 cgtgatggca gtaaacatag cagataagaa aaaacaagtg aaggaagaga ttgaaaaatt
     6721 acagcatgaa ttaaagatag aattgcctaa acgcattgcc gaagcacgat caaaagggga
     6781 tttgaaggaa aatgctgagt accatgctgc ccgtgaacgc caatcgtttg tgcaggcaca
     6841 gatttcatat ctaagcaaac agctcagcct tattgatagc atcgacatat ccactattga
     6901 acagggaaag gttgactttg gctctgttgt gactgttcgc aatctggata atgatgaggt
     6961 tgcccggttt actatcgtta ccccggatga ggttgatgct tcggaaggca agatatcatt
     7021 gtcatcgcct ataggcaggg cattacataa aaaaactgtt ggcgatgaag tatcggttat
     7081 tattcctgca gggaccaaac gcttcaggat tgaaaagctt gttaccatac atggcgaaga
     7141 actatcgctt tgaaaaatta aggaacttgt acatggcaac tatcaaagaa tttaccgcat
     7201 tgcggccggg gaaggatgtt gttacaaagg ttgcatcact tccgtatgat gttgtaacag
     7261 cacagcaggc aaaaaaaatt gctcaaaata atccctgtag ctttttctat gtaacaatgc
     7321 ccgagataga tttaccattg tcaaccaatc cttattctga cgaagtatat aaacttggga
     7381 aaaaaaaatt aagcaaattt ataactgacg gcataatggt gcaggatgat aagccctatt
     7441 tatatctcta taccttacag attgatagtc acaaacaaac gggtattgtt gctgttgtgt
     7501 ccattgatga ttatataaat gggatagtaa aaaaacatga gcttacccgt aaagccaaag
     7561 aaatggacag gatgaatcat atcaacattg ttggggcaca aacagggctg gtgtatcttt
     7621 tttataagga tagcaatgat ttacggaata taatgcatca tgcattatca tcacctgtac
     7681 tttatgattt tgtagcagat gatggtgtgc ggcacataat tcaaaggatt gaagatggtg
     7741 attacgcaca attggtaaaa caagcttttg atgaacaaat cctctatatt gctgatggtc
     7801 atcatcgcgc agcttcggct gtaagggttg gattgcaacg ccgggagcag ggatatactg
     7861 gcagcgaacc gtttaattat ttcatagcag ctatctttcc tcattctgag ttaaatattt
     7921 tgccgtataa tcgtgtagtg aaagatttat ataatcatga ttttgaaact tttatgcgac
     7981 ttctatctga ttcgtttgtt gttcaaaaaa ctgataatgg tattgcgaaa aatcggcatg
     8041 agatacggat gtactatgat catcaatggt atacattaac ctttattaaa aaattgccac
     8101 tggatgcggt tgatgcattg gatgtttcta tcctgcaaaa tacaatactt agtcctattc
     8161 tgggaataaa agatccacgg gaagatatgc gtatagactt tgtaggtgga accaatgcta
     8221 ttgctacaat ggttgaattg gtggatagtg gcagctatgt aattggtttt tcgcttttcc
     8281 ctacgtcaat tgatgagctt atgacaatat cagacagggg ggagataatg ccgcccaagt
     8341 caacctggtt tgaaccaaaa ttgcgggacg gcttgctggt atattgtatt gattaacgcc
     8401 ttggagacga ggggaatcga acccctgacc tcttgaatgc cattcaagcg ctctcccaac
     8461 tgagctacgc cccctgattg ttgtgcagtg taactttatc atttatttaa aggaataatt
     8521 atcagaaaat ttgtcaatat attttaaata tattttaaat atattttaaa tatattttaa
     8581 tagtacttat aaaaatttac tatctgtggt gatttattct tgcaaaaatg gtgattgctg
     8641 tgatactata gctatacgat atgcatgata gtggggctga actattacaa cataaaatat
     8701 tttgaggtta ggttatgtta tcagaaattg ataagcttaa aaatgaaaat gaaattatgg
     8761 caaaagagat tgatgagctg cggaagatag tgcagacata tgaaaatgtt ttaaaactca
     8821 atgaacagga attggccaat gcctatcaaa tcatatcaat gtatgaaaat attgtagaat
     8881 attcccgaac cgaattaaag ttggcaactg aaacggctca ggcacgtgaa agtgcatcac
     8941 agttaagcag gatggaattg atgcaggcat ttgaaaagat tcaaaagctt gaagaagata
     9001 ataaaaaact cagagccaaa aaagaaaatg catcctgata aaattaagat gaaggtaatt
     9061 gggtagtaaa aacatgttga ttacgtatgc tataatattt ttgttcatta taattctggt
     9121 aatatttgtt ctttattttg ctaatattta tattttcccg cgtaagctcg aagaaatcca
     9181 gcaacttatt gagtcagggc agacaaaaat agccatcaga aaattaaatg atattattga
     9241 aaaagatgat cgtaatcatt atgctcatta tttgctggcg ttagcatatg aaaaagaaaa
     9301 taatattcag tatgcaattc ttgaatatcg gcaggtttta aaaattggaa gatatgatga
     9361 aaaggttaat gaagtcaaca tacgcaatca tttagcaagt atttataagg cgcgaaatgc
     9421 agtagaagaa gcaaagaagg aataccttct tttaacaaaa ttggatcctg caaactttga
     9481 acctttttat gaacttggta tcatttattt taatgcagga cagatagata aagctatccc
     9541 gtattttaaa aaaagcattg caaataacaa caagcatgga gcttcatttt actatttggg
     9601 acaggcatac tatagaatgc aaaactatcc tgatgcaaag caagctttta ttgaagccat
     9661 taaggttgat ccacatgatt ataaagctca ttacttttta ggtttggttt tgcgacagtt
     9721 aggcgattat gaatgggcaa ttaaagaatt tgagacagca cagaaaagcg atgacattaa
     9781 ggttaaatgt ttccttgcta aagggacatg ttatttagag cgtgagcaat tgccaaaggc
     9841 agtgattgaa tttgaacgtg gtttaaaatt tgctaaaaga ggtagcgata ccgagcttaa
     9901 tttgcggtat tttctggctg aagctcagga aaaaatgcga gatttacact cagccatatc
     9961 aaactgggaa aagatagttg cggtcaatcc tacgtttcgt gatgtacagg caaagcttaa
    10021 atcgtatgca gaatttagac aggatgatcg tattaaagat tttatgattg cagggctggc
    10081 acagtttgag catacctgcc gtaagatatc tgaatcaatg ggacttactg ttatggatgt
    10141 agatatcatc agtgatacag aagttgaaat catagccacg gatactgaag gaaaatggcg
    10201 taatacacgc cgtactaata aaattgtacg aatacttaga actaccgatg ttgtaaatga
    10261 taaattgctg cgcaaactgc atgaaaagct taaagaaaag aatgcaacac gtattatcat
    10321 tattactgca ggtgatttta cacaaagtgc tgttgatttt tcaaatacca ggcctataga
    10381 gttatatggg aaaaatgatc ttttacgatt attaaaacaa atataattgt atggtaaaca
    10441 aagcaatact tttattgaat caggtgccaa tcttcaaaca tttaatggca gatgagctaa
    10501 cagctttatc gctattagct aaaccacata ccgttaaaaa gggaagtacg ttccattgta
    10561 atgctcagtc gatatacatt gttgagaaag gtgtttttgg tgtacaaaag tcatctgaat
    10621 caatatattt atcccgaaca tcatattttg gtcagttacc attctgtgaa aagagcacag
    10681 gtgttgtcag ggcattgagt gatgcatctg ttctggagtt tgccaatact gatttatatc
    10741 aatttatctg taataatttt aaagcactgc gcggttattg caggatactt gatcattata
    10801 gaatcccgct cagtcaaact gctacaacat tattaaaaaa taaagcctgt gtatacacta
    10861 tttttagtac tgataaacat tgtggcaaaa caacactggc aatatcagtt gccactgcct
    10921 gctcatacta taaaagaaca attttgctgg atgcctcata tgaagggata tctgtatttg
    10981 acatgtgtgg taaaccactt ataccgccgc tgtcgcaaaa aaatcccgat gatgtcaccg
    11041 gttataaatt tattcagcag cgtatagaaa aaatttcgga aaccatggac atcgccaata
    11101 ttgcctatgg gtcaaaagta aaaataaatc ctgatataat aaatccgcta ttattttatt
    11161 tatcatttga atattcatac atcgttattg attgttcaaa cagtgatatc catctgcgcg
    11221 atgtgataat gaaaaattca gacagtatta taaatgtcat taatgaaaaa tctgccatta
    11281 atcatcaagt agtaaatgat actcttttaa ctcagggaca acaggtttac aatgttgtta
    11341 ataaacattt tgtaaaaata caaaaaattg atcagccatc ctatgtgttc aaaactattc
    11401 cagtagaaac aaatgaaacg atttttcagg cagtattaaa aaataaaatg tataatgatg
    11461 agctatttaa aaatatagta agtgataaaa gggcgatggt attgtcccat gggatgtatg
    11521 aaacgctata ctattccgga gtattggaat cattgcggga agaaaaactt tttgatgtta
    11581 tatatgctaa tggtataccc tatgcagtat tgatgtgtta tttggcaaca caaacaacag
    11641 aagattataa gaaattattg tttgattatt tttctgatga ttcattacat acattctttg
    11701 atattgcgtt tcctgataag tatctgttta aaaataaacc attgcacaac tttgcttcag
    11761 ctataagtca tataaaaaga attgaatgga atgcaatact tccttataca cagttaatgc
    11821 ataataatac gcaaaggttt ttctctaccg gtgatgctga aaaaattatt tccgctaatt
    11881 tagcttttag tcccttattt gaagggatat ctattcatgg tgatgcctat ttttctgctt
    11941 ttccgcttta ccccctgatt cccgagtcat tactgagaac gcatataaat tatataggtt
    12001 acattgctat tgctaatccc tataatccaa tttctgtaaa aagtaagatt cctggattat
    12061 atagaaaaat tgttgatttt cttcctgatt gttatttata tgcctgtgat aatacctttt
    12121 gttgcaatag attaatttct ttaacgttac ctgatgtcac atcaacacat gatctgatta
    12181 aacaatcgct taaaaatact gatatgttta tttcaatgat aaagaaaatg ataaagtaaa
    12241 tgataaagta aatgataaag taaccaatta ggagggtgca tgagatcaaa agctaccaat
    12301 atcttacaaa caggtgttct tttaacaggc attatttata tcattattgg attattgtat
    12361 ggtttcagtc ctatatactt tgctaatatt tttggtatag aggtaaatcc cgactggtat
    12421 aatttaataa aatacgatac atttatagct ccgctttatc acttttccag gatatttgct
    12481 ttattattag ctattgtagg cataagtatg ttgctaccat tatttgatcc attaaaatat
    12541 cgtgggatga tatattacca tagcatacta tttcccttag tttctgtgcc atctcttttg
    12601 ataaatggta ttgcctataa acatttgcct cttacaatat gtggttgcct tttattgata
    12661 atttttctct ttgtaggttt tgggttaata attacaaaaa aatatgcaaa aatgggactg
    12721 gaataactat aataaatgct tgctttttat aattgaacat gtattgattc actttaataa
    12781 taaaaagaat ttttatgcag ctttacttat gttatttcta atatttttat caggaggata
    12841 tgatgaatct gaaaactaag aaggttggga atattgtagt agtttatcta cagggacgat
    12901 tggatgtcca tctttcagcg gatatagaaa aggaaatcaa taaattaatt aaagatgatc
    12961 ccgcttctca tctactgctg aatcttgcag gtgttgaata catgagcagt agtggtttaa
    13021 gaatttttgt ttctactatg cgtatattaa aagaatcaaa tcgcaaatta aagatatgtt
    13081 caatgaataa cgctgttaag aaaatttttg aggttgtcga actcatggat atgtttgaaa
    13141 tctatgaaac agaagaagaa gctattgctt cgtttaaata atgtttatgc tgtcgtcttc
    13201 aggtaagtcc aaaagttcca aacaacagtt atttaaaaca ttgctatcgc aaaacgctga
    13261 gtatgagact atggcaatac ctttggaatt atctgaaggt ataaaaaatt ttgaaattta
    13321 tgtaaaacac aactattttt catggccata ctggatagct cattatacta atagggaaaa
    13381 tgttagaata cagtctctcc cgtttatttt atataacaat cattgcagaa catggtatac
    13441 aataaatagt tttttaaaca gtggagcaat aaggatagat ccttgcggta tgattcatcc
    13501 tgactgggca aaatggtcag ttgaatgtat ggtctactct gatggggcat atattaaatt
    13561 tcatgatgat acatgtatac atcaacaaaa tgaaagggga gttgtaaaaa catcgtatca
    13621 gcatcattta tttcgttttt tacagtcagt gtatattgct catacaaatg ttgatgaagc
    13681 gatagttatc aataaaataa atattaaaaa aaagaaccaa tcctatttca tcatagcaat
    13741 cagaccttat acaacaacaa cgttatccgg catcaattca atcgagttgt taaaaaataa
    13801 tttagtgaaa atcaatgatg atcatgcatg ttcattaatt gaaaaaccgg attatattct
    13861 tgttggtaat gaaatcatag gtgatattga tatattacag aaagatgata aatataggat
    13921 aagtagttca agtggtatgg cgacgatggc ttttgcatat gaattgaaaa atacggatca
    13981 tgaatatggt attcgcatcg ctaccggtaa aaatacaaaa ttatccgttt caacattatc
    14041 tgttaaatca gctcttaatg attataccag ctatgtagat agcataatga aacaggggat
    14101 tgccatatca attggcaggc atactataaa cgagtggact gatttttgca aattatcaac
    14161 attggtgcat gcctctacta tggcatctat aaaaacaact caactgcaaa ggttgaagca
    14221 atattactat atttcttcaa ccttgaacag aatgggttat ctaaaacaaa ctgagcttgc
    14281 cattgatact atcaaaaaaa atataaagat tacggatgat tatgaaggtg tcctatcttc
    14341 atcatttttt tgtttaatag ttggcgattg ctataggaca ataaaagatt cctcttttgt
    14401 gcaaaataat tattccttat gcaagcaata tgcttattcc atcactgaat tttttaaaaa
    14461 aataggtgat ataacaaaag aaaataattt taaggataat cctcaaatag ccataaatgc
    14521 gattatcaat attgcatata ttatatctgc tcttgaaaat gtagcattta tgtcgcgttc
    14581 gttggggctt tttggtgagg aaacaaaaat atttagtgat ataaaaagac tgcagaatgt
    14641 aatattaaag tcaatattta ccataaacag tgaaacaaac ggtgaagagg agaaattaat
    14701 tgagtggact cctaaatata gtaaacctat acgaaaacaa aaaataatag taaaagacag
    14761 ccttgagttt tcccattttg taaatgtaaa tgataacagg attatatcat acagcccctt
    14821 gctttaccct tatatgaagg atataatgca accgtatata gtgaaaaata taattaatgc
    14881 tatcatacat gcaggtaaag gattgccttt atataacacc ataataagcg cggttgatat
    14941 agtaaattct ttaagtcttg ctaatgcaat gatctatgct catgataaaa gagctatcga
    15001 tagtattaat gcaattatta atttatgtaa taaacaaaaa tttttacctg attttcttga
    15061 tcctgtaaca ggtcatgccg ttgtaaatga ccctgtttca actattgctg tttcattatt
    15121 atttcaagct atccgtaatt tatgctttat agatcatcct gaacgactgg aaattttccc
    15181 ggttccatgg gaagaatggt ttaatgattg tcagatgaat ataaaaaatg ctcattcacg
    15241 gtttggtata attagtttta cggctacatc cacatcaaat gaatttgtaa ttcaatttac
    15301 tgacataccc cggtttatac cacatgatat tttagttaat cttccattta aagcaaaaat
    15361 aaaaaaatca gatgatttta ttgtcaaata tcagacagaa aaatcatggt gtattaatgg
    15421 ctggccttca gagattagat ttaaacgtta attactgttc tatcgataat aaagtagtaa
    15481 ttgcggtggc agttgtaatt ttaatactgg aaaatgaaaa atgatatcat atcttgtaat
    15541 atcactatta attatcagtg gatttgtata ttatttagta tatatcagac caaaatttaa
    15601 tcccttaaat aaggcagaac aatttttaaa aacaaatcaa ttgcatgatg caattattga
    15661 atataaaaaa gtcatcgatg aatatcctga taattttgta gtccatcatc gtctggctga
    15721 actttattta acaatgggtg aaattgatca ggcagtgatt cacttagaga aaatattaga
    15781 aataggtaaa tttaactatg aggttgatca actttctgta ttaaaaaaat tagcaaagtc
    15841 atattatgtc cgtgatgagg ttgaaaaagc attcaagatg tatatggata tattaaaaaa
    15901 ttttcctgct gatattgatt ctctttacca tgtatcattc attttgttaa gtcaggagga
    15961 atttgatatt gcacaacgtt attttgaaag attattaaaa cttaaaaatg atagttacga
    16021 gattttattt ggagcaggaa tctgtaatta tcaaaatatg cgaatccagg aagcgattga
    16081 taattttaga ttggctctac aattaaaacc acaatcagaa atagctcaaa ttgcattggc
    16141 ttttgccttt aaccgtaaac gtgattaccg acaggctctg acgtatatta caaaagtggc
    16201 tgaatctgca caggatcctg acgttcagta tttagcaaaa cgattccagg cttttttata
    16261 tatgcgcctt aaacgtcatg atgagggtat taaactattt caggatttac tggaaatagc
    16321 ccgaaagaat gctaatgatg atgctgaagc tttagcttta catgatcttg ggtatgcttt
    16381 gttaaaaaat gaacaatcat cattagcata cgatgtgtgg aatgaactat accgtaaaga
    16441 tcgaaattac aaaaacatac aatttcttgt gacgcaattg cgaaaagaaa tggaggctga
    16501 taaatttgtt aaagaagaat cagcctctat aacagaaact attacagcat ggatggagga
    16561 agcttttcca caaaatttac tatgggatat gtgtggatta aaaggcagta aaagatacaa
    16621 tttaaaagat gttgttgtga caacacgtat aacttttgat gatgggaagc tatccagtac
    16681 ttctgatgag attatacaaa agtatattgc tttagatact gaaaatttca ggattatatc
    16741 aaatagattg gtgtcaaaag tgggatttaa agtagatcag attttgcaaa cataccgtga
    16801 agctgatggt gttgattttt tagctgttaa tcctgaaaca aaagataagg tgcttatatg
    16861 ggtcaggaga tggacaaaga caaatgttgg tgaaatcacc ttgaggaatt ttgctcaggc
    16921 aattaatgat atgaaagtta agcaggggat attaataacc acggctgatc tgactgatat
    16981 tgcaaaggaa actttaaagc aactatcaaa gatatcagta atctatcctg atgaaataga
    17041 aaaattactg cgaggacttc tttaaatgcc atttgataat ttcacatcaa caatgatacc
    17101 tttgcagtca attaattata tcaatgatta ttacaaaatt tcaaaaccat tgtctcctga
    17161 attaataaaa tcaataaaac aaaacggtgt gctggaacca gttatcctga ttgagggtaa
    17221 cggttgttat attccggttt gtggtcacaa caggatgctt gcatcaattc aaacagaatt
    17281 ggccaatgta ccagcaaaaa taatcaatgg gctttccgaa gatgatttca tggctataat
    17341 aacattaaaa ctatacaaca atttaatggg aactatcggc aaattaaaaa gtttatttat
    17401 actaaaagag tattttaaaa taaataaaaa acaattgatt gcgatagctc atgaattatc
    17461 agtcccagat gctttgattg aaacgacttc tttaaaaaaa attattggtc tgccatcaag
    17521 tttaatgaat tatattgatt caaaaaacat accttttaaa tttataatgc ctgtccttcg
    17581 cttatcagat gaaagtattc aacttgttgc tgcaatcata aatgcctgtc aactgcgtgt
    17641 aaattatttc agggatattg ttgtgatgat agaagatata tgtcgtaatc agcactatga
    17701 aagcgttttg aaaaaaatta ctgttctgat caataaggaa aacagaactg atgatgagat
    17761 atatcgaaac atctatttgt tacgatatcc acagtatagc gaatatgtag aaaaaattaa
    17821 ttacttaaag aataaatttt taagttatgg tttaaagata gaagtacccg aataccttga
    17881 aggtaatgag gtgaacatta ttattcaggt gaaaaagaat gaagttgtac acccgctgtt
    17941 acagcaggac aaagaaatac gtgcgttgat tcaatcaatt attgaattgc tttaaattaa
    18001 tggatttatt taaaaaatgc tgacatctct tgaagaatct tttgtttagt agtctcaaaa
    18061 ttgtaaggag atttaaaacc agcggcattt ttatgtccgc ctccaccgtt tgctattgca
    18121 attgctgcaa catctaaatc gcctttagtt cgcatgctta ctttaaccgg accgtcaaca
    18181 tcctgtttta caacaacact ggcaactaca ccattaatag ttaatggtaa attaatattt
    18241 acttcgcctt ctgaaaaagg tgcattcgtt gctttaatca tctctggagt taattccatt
    18301 aatataagtt ttccaccaaa atgtacttcc atggatgaaa gcatcaaagc acgcaatcta
    18361 aatacttcta acgtgttatt ttcatatatc cattgatata cttcgtatgg tttaattcca
    18421 gatgaaatta attgtgctgc aatagtaaat gtatgtgatg atgttttagg atatttaaaa
    18481 cagcccgtgt caaaaacaat accagcatag agtgcctgag ctgccttaaa tgatggtttt
    18541 atgttatacc gttccattat cgtgcagatt atttctgatg ttgatgatgc ctcaacttta
    18601 atgtaatttg tttctaattt attgttagca ccttcatggt gatcaataat aaaaacatct
    18661 ttaacaatat tgtttaaata atggtacgct tttccaatat tattaaaatc atttgtatcc
    18721 aatacaaaaa tagtataatt acttatattg tctatgatca ttttttcatc aaaaataatt
    18781 atttcattat caggatcaat aaaattaaac tttgtgggag ttggatctga attaataatg
    18841 atagcatgtt ttcctaaatg tagtagtatt tccttaaatc ctatttcagc gccaagacca
    18901 tcaggatcag ggctctcatg tgttgatatt ataaaatgat catgggcatt taaatatttt
    18961 tcaaggttgg caaagtaatc cattaagctt tctcgggtga tgcatatttt gcatatttac
    19021 aaagcggaaa tcccattgat tcacggatag caacatatcg ctcggcacag cgtcgggcaa
    19081 tgttgcgaac tcttgtgatg taacctacac gttcagtaac tgaaattgca cctcgagcat
    19141 caagtagatt aaatacatga gaacatttta aaacataatc ataagccgga agtattacct
    19201 gaaccggttc ataatcaagg atcttcaggc attctttttc atagagatca aatagttgaa
    19261 ataataatgc ggtatcagca atagaaaagt tataatgtga aaattcaaat tcactgcgat
    19321 gatgaacatc tcggtaggta agtgtatcat tccattgaat gtcaaaaacg ctctcaactc
    19381 cctgaatata catgcagata cgttcaagac cgtaggtgat ttctaccggt ataggatcaa
    19441 gctcaatgct tccgcattgc tgaaagtaag taaactgtgt aatttccatc ccatcaagcc
    19501 aaacctccca tcctaatcct gatgcaccaa gtgtaggtga ttcccagtcg tcttcaacaa
    19561 atctgacatc atgttcttca agtttgatgc ctaagttacg taatgaatcc agatataaat
    19621 cctgtatatc aagaggtgat ggtttcataa ttacctgata ttgataatag tgctgcagtc
    19681 tatttggatt atcgccatat cttccgtctg ttggacgtcg tgatggttca acatatgcta
    19741 cattccatgg ctcaggacca agtacacgta aaaaagttgc aggattaaat gtacctgctc
    19801 caacttccat atcatatccc tgtgcaatga tacaaccctg ttttgcccaa aagctattac
    19861 aactggcaat gagatcctga aaatacatgc gatagtctcc tgaaaataga attatgttac
    19921 aatgtcattc ttagggatgc agtaaagaag aatttcctta acaatgattc cataactaat
    19981 ttgtgatctt attgttttgg gaatattctt caaactttgc ttttattaac tggtgcaatt
    20041 catcaaaatt agttttatta ttaagcttaa aatagtcaag acagtcttca tctgcaatct
    20101 caaatctaaa gttttttgac tcaatgctgg taaagacata tgccagatag acaatgtaga
    20161 tcaaatctct gtactgcgag ggagccagat gcggtctgtg atgaaattca atagccttca
    20221 ccagcgcatc attaaatttc catttctgac atatcaaact tcctaatgtg ctatggctta
    20281 ctcctaagga tatctcttcc agtattgaga tattatcaag ctgttttaag cctacaattt
    20341 cccgtatttt ttgaatttca tctggtttga tagaaagtaa taccataagc ccaatatccg
    20401 aaagcaatga tgccagataa gcaaattcag aaagccgttc cttgcgatat ttcatcgcga
    20461 tccattgtgc ataaaatgct gttttatatg aattgttcca gatggtttca aattttttat
    20521 agcgggaatc aattatcttt tgaacaccac tggcaacgag cagtgtattg ataccttttg
    20581 tgccaatgat ttttactgct tcctcaatag tttcaactcg ttttgatgtg atgtagcctg
    20641 ctgaattggc aagcttcagt atggcggtaa caagccccgg gtcgcgcttg atgctgtcgg
    20701 ctatagcctt tattgtagat tcaggatttg cacataagcg ctgtatttct agaatatttt
    20761 gtggaaatga agggagtgat tcaacctcat ttatgatttc ctgtgccatt ttaaccttta
    20821 gatccatgtt gcggaagctt tcatcaatgt ttatggtggc actggtgatc ttaccatttg
    20881 tggacatgct aaatgcctca ggaggaaaac cggcattctt catgagtatc aatgtaataa
    20941 tcaaacctaa tcctgctcct tccgaatcat ctaaaatgtc atcaaatgca tcggaaatac
    21001 catggtaggt atatgctttc tttattcgtg cattaatttt gttcatctct tcaggtataa
    21061 tgggaacatt gtttattaca tgaattttat gaatatcacc ggtaatggaa aagaataccc
    21121 gtacaataaa gttggatttt ccaagctctt caagaaactc gggatttcca acaaacacat
    21181 cttctttaaa tgtttccatc ccgttacggt attcttcttt tttattgata tccagatttt
    21241 ttcgtttaaa gtaaagccgt ttagcgttag ccttgatggc attattcacc agttctttta
    21301 gtatgtttat aaacatatct tttaaatata aaagatcaag ttgtgacaaa taaatactca
    21361 gcactctgat taaacatgct tcaacctcag gggttaatag tgaaaactgt aggtaaacgt
    21421 cttcacctct tgttaatgct tctttataat caactggctt acaaaaataa tgagatgttt
    21481 gctgtgttcc ttccaatatt tacctcatcc aaaaaatata acaaaacaat acagggtatt
    21541 gtgaatattg caagaaaatt acaatgaaac gtcaagtttt tattcaatta tacttttata
    21601 tagtgtatcg gtttttttat atttttatta atggaaaatg gtggcgatag taactctcaa
    21661 ttttgttcat tattgtaaat agacttttgg tggaaatatt tatatcgttg taaatctgat
    21721 tgcattgacc ggtatgtgtt aaaatgtatt gaatattttg attgttacca ctctcatgaa
    21781 catacaatag tgataaatga tttttttgtt ttatagacta ttgttgatac tatatactat
    21841 ttttttaagg tacctataaa atttgtgcat cagcaatgaa tggtgatatt aatgagtatt
    21901 gaaaactgtt attgacaaat catggtgatt atactaatac tatcagaaac tgttgtgcaa
    21961 gcgaggtaca tatgtctgtt gtttatttta cagatttgca tatgagcagt aaggagaata
    22021 tcgtcagcaa aattaaaaag cttttttaca aattatctgc aaatgagata atcaatgaaa
    22081 aagattttgt tgcaatcaaa acacattttg gtgaatatgg caacattgcc tatacgccgc
    22141 ctgtaataat acatacattt gttgattgtg ttaaggattt gaaagcaaat ccatttgtta
    22201 ctgattccac cacattgtat cgaggaagca gaagtaacgc tgttgatcat ctgaaaaatg
    22261 ctgctatgaa cggattttct ttagtttcat gcggtgcacc ggtgattatt gccgatggat
    22321 tacgaggaaa cgattttaaa gaactccctg ctaccggtaa atggtattca actattaaag
    22381 tagcttcagc aattcacgat gctgattcga tggtagttgt gagccatgta aaagggcatg
    22441 aactgtatgg ttttggtggt gctataaaaa atattgctat gggatgtgcg ccaccttcca
    22501 tgaaacaatc attgcattct gatttaaagc ctaaagtaaa acaaacacta tgtacctcat
    22561 gtggtgcgtg cattaaacga tgtccggcat ttgccatttc atttgatgca aataaaaaag
    22621 catttatcaa tagtgataag tgttttggtt gcggagaatg tacagttatt tgtgtttata
    22681 atgctatacc ggtattgtgg aaaaccgacc ataccacact gtatgagcga acggctgaat
    22741 atgttaatgc ttttatgaaa cataaggaaa ataaagtttt attttttaac tttatcatga
    22801 atgtatctcc tgaatgtgat tgttgctact ggaatgatat ccctatagtt cccaatatag
    22861 gaatagttgc ctcaaccgat cccattgcag ttgataaagc atcaatagat ctgataaata
    22921 aagcaactcc gatgccagat tcacgaatag ctcataagga tagctcaaaa gataatattg
    22981 aagcgttata cccggttaaa tggaactgtc tctttgaata tgctacatct attggagccg
    23041 gtaatatgga atatgaatta gtgacaatat aacattgata tttttataaa atggagtgag
    23101 gttttatgaa aagtccacat gattttcctg caatcaatcc taagagacct gatggtataa
    23161 aaccaaacgg gataccatat aaggttatgg taattgatga taaagagttc catcgcaagc
    23221 agattattca aatactggaa tctgaaggat atgagataat tgctgctgct tccaatggag
    23281 ctgaagcttt gaagctatat gaatcactgg ctgctgatct tgatcttatt acaactgatc
    23341 tggatatgcc aaagttagat ggatatgcgc ttatgtatga attaagtcat aagaaaccaa
    23401 aagctaaaat tgtgttcatc agtgaagaaa ccacaaaagg ggtgctgtct gacttattaa
    23461 aaatgggtgc tgctgatttt attttaaaac ctatccagcg tggaacaata ttggagcgga
    23521 tcaaagcagt attacaaaaa aaatgatagt atactatgaa taccatcaat tccattttgc
    23581 tattacattt taactgataa agtctattgg tataaaaaat tgatataaag cacttttcac
    23641 aggatagtat ccatggaaga aaatgataaa gcttacatgc atctgtctga acaaacaaga
    23701 aagataaata ttgcaatgca ttatacccat ggtgatatgg aaagagcccg ccagatggtg
    23761 tcagggaatt acaatgattt atatgctatc aaggttcgtt ttgcatcttc aacactgttt
    23821 ggagctttct tgcttttttt taatactatt tataattcaa tttccggatt atcgctcatt
    23881 gtatcacctt cctatacact gctgaatata gatccggcac tggactggat actatttgaa
    23941 aaagaaatag caaaacagat gcaaaaagat gaatatgacc atgttcttgc taaaactttt
    24001 agtgataaaa tgattaaaaa ttttagtact attttgcaaa atgaattatc ccgaaatatt
    24061 gaaataaaaa atgatatagc aatagacaac ctattcagga agcttattca ggagacattc
    24121 gcattgcaac gtgttgattc acttgtagtg tatcaatcaa catcatccct gacaatggaa
    24181 ttgaattcaa ttactatccc aaagttggaa tctgtaaagg tggaagggga gcaaacacag
    24241 gaatcagagg taataaaagc agatactccg tcaggcgaat atacaccatt ggtgggtgtt
    24301 gatggtgtta agcttattat aaaagcaaca ttaattttat cacccattaa agggaagcat
    24361 atttcacaat tatcagccgg agacagaatc atgatttcta ttatcgatag taaacctgtg
    24421 gcggtaaagg ttgcaaaggc attgcaagcg tatcaagatg aacaatttaa acccatccct
    24481 gcgcgcatta cacatgtcca atgtattcca caaaagggat ataaaatcta tgggcttatt
    24541 gcaaaaggca ttgttgttga tataactgag gaagaagaaa atataaaggt tgcattggat
    24601 ccaacgtatg ctgctacagt gcaggcagca gaggaagagg caaaaccaaa ttatattata
    24661 atagcaattg ttgcagttac atttttaatt gtaataggaa ttctgtattt attgttaaat
    24721 gctaactaaa atctaccgga cgaataggga tgccgtgtga agctaaaaaa tcctttgcct
    24781 gcttgatgct gtattctcgg tagtgaaaaa ttgaagctgc cagtactgca tctgctttac
    24841 cataaacaaa tccttcgtat agatgctcta aagttccagc acctcctgaa gcaataaccg
    24901 gtatatgcac aatatctgca atgctttgcg ttaactttaa atcataacct gctttagtgc
    24961 cgtcccggtc catgctggtt aaaaggatct cacctgctcc cagtgcttcg gcttgttttg
    25021 cccattgcaa tgcatctata tcggtttttg ttttaccgcc gtgcagataa acattccagc
    25081 cggcactgtt ttgtttagca tcaatggcaa caacaataca ttgagatcca aatcgcatgg
    25141 cacattgggt aataagctgt ggcattaaca ctgcctgagt attaattgat actttgtcag
    25201 caccgttttc taaaatcatc ctgacatcat caatagtacg tataccacca ccaacggtaa
    25261 aagggatagt aaccgtttcg gcaacatctt ttaccatttt tagtatgatt gcccgttggt
    25321 cgcttgatgc ggtaatatca agaaacacca gctcatcggc accttcagca tcgtagaaca
    25381 taccgttgtt aacaggatca cctgcatctg aaaggtttac aaaattaaca cctttaacaa
    25441 ccctgccatc tttaacatcc agacagggta taatacgttt ggctaacatt aatagcaact
    25501 atcctataaa attttgtatt gacataaggt ttagatcaaa agggacaatt gtatcaaaac
    25561 tcatttgaca ataccatagt caatctatca aatggaattc atatgtcaaa aaaaattgaa
    25621 aaacataata cttttcatga cttttccagc agatatgttc cgctcatcaa tcagtatatt
    25681 gacagttttt ataataataa agaactatct gccaaaaatc atgagctaaa aattgcatat
    25741 agactgttac aggaatattg tactcgcgaa ggcaagcgta tccgtcctct gttgcttttg
    25801 cttgcttttg caggatatgg gggcttggtt aaaaaaatta atgcaatgat tcctgttgca
    25861 tcgtcacttg agtgcatgca tgcatttttg ttgattcagg atgacattat tgatggtgca
    25921 gttgtaaggc gtgggaagcc ggcattccat atactgtgtg acagctcata ttatcagtac
    25981 aatgcaaata ttggtacatg ggtggcgata gttcttgctg atgttgttat gagcaatgca
    26041 cttgagatga tagcaacact ggagagcagt cgtataaaat attttttgaa tgaatttgca
    26101 gtaatgtatg aacgtaccgc gttaggtcaa ctgcttgata ttatctggtc acatcctgaa
    26161 acaatcagcg atgatggcat agcggagatg attgctacaa tgaaaacggc tcactataca
    26221 gtatctggcc ctttgaaact gggatatata ctggctggtg gtaatgatag taatgagttg
    26281 caacggctaa cagaaatggg ctattcattg ggatttgctt tccagatgcg tgatgatatc
    26341 ataggagttt ttggtgatga aaaggatatg gggaaatcag caagttctga tatagccgaa
    26401 ggaaaatata ctgtactggt acaacaggca tggaacaaat taaatgaaaa agacagaacg
    26461 catttccttg atttattaag gaaggatgaa aaatcaagtg aagaaataga tacaataaaa
    26521 cactatataa caaaaagtgg tgctgtcaca gtactccatg ctatcattga tgaacatctg
    26581 caaaaggtgc atgccatatt gcctcaactg cagtgcaatg atttcatgaa acaatgcatg
    26641 ctgcaattta tagaggggct gtttcaataa cagtttacca tccagttttt ttggctattg
    26701 tttccctgcc ttatttacgg ggaggaatca agggtgaggt ttctatcatt ttaatccatt
    26761 caggtagctg catgaagcca cccgcagccc taccaataat aatgtaataa ataattattt
    26821 tcttgtcaga ttgtaaataa caatttactt tgtaaaatta attgacgaat acattaatta
    26881 tgagtttagt ttgaaaagaa tataatgaat tatggagaat aataatggct atggttgcca
    26941 aaaaagctca taaagaaaca tttgaaagga tttcgcctga aaagcgggat aggataataa
    27001 ccatcgcgac aaaggagttt gcaaaaaatg gttttaaggg agcaaatatt aatataatag
    27061 caaaaaaagc tggtatcagt attggttcaa tgtataacta ttttgaaagc aaagaagatt
    27121 tactgttaac ggttatcaat gaggcgtata aattactgga gaatgtgctt ggttcggtaa
    27181 gcctcgaaaa taaaaatttt tatgaaaaaa tagaagagct ggtatgcgca gcacaaaagt
    27241 ttgcccgaca atatccggag attcatcagg tttatcttga tataacgtcc gagggtttat
    27301 cacatcttac accgcgatta tccaggacaa tagaatatat taccattaaa ttttatcgcc
    27361 acgaattaga gcaggcaaaa atacagggta tcgtacgaga agatgctgat gttaatgttg
    27421 cttcactatg tattgataat atattagtat tgacccagtt ttcatataca tctgaatact
    27481 tcaaggagcg attaaaactt ttcattgggg aagatgcatt tgatgatgat gaaaggatag
    27541 taaagggtgt ggttgatttt atcaaaaatg cattgatgcc caggatgaac atacacaata
    27601 ataaataatt atctctattt tcgattagaa aggttgtctt taaatgaaaa ggaaaaatgt
    27661 aaatgtcata gaactacgaa gaaatcagct tacccgtgcg gcatataaag ttgtaagcag
    27721 gaaaggatat tataatttta ctgttcgtga tatagcaaaa gaagcggggc tttcaacagg
    27781 tctggtgcat tattatttta aaaacaaaga tgatctcctg gtgtctgttt taagagtaat
    27841 gaatgaaaat ctgagcagct atcttgcaaa agcacttgaa aagatagatg atccagaaga
    27901 aaaaataata atctttatgg atgaagcgtt tcaccttgta gagcgtgaaa aagaatattt
    27961 ccacgtcctt attgatttct ggacacagat aaatcataat gagcgtatga gaaaagcaaa
    28021 cattaaatta taccagagct atagggcaga atgtgctaaa ataatacagg aaggtattga
    28081 taaaaatgta tttaaagatg tggatgtgca atataccgca acgatgattg ttgcttttgt
    28141 tcagggactg attatacaat atattattga taatgaagca tttgattata ctgaatatac
    28201 aaagaagatt aagttgcaga tagtggataa cttgatgaag taatcaaggg tgttgcaaaa
    28261 ttttgatttg tatgctatat tgcgataaac ttactgacca aacatggtcg taatggtata
    28321 tcaggattac agaattatat gttaaaagat atttcagaaa atggaaatta ccagaagcat
    28381 ttagaaaaca tagtaaaaac attatgccag cctcagtcat ataaaaaggt atatcaccgg
    28441 ataaatcacg atgttcccat gccttcaatt cagcaattag aagagattgt tgaagaatgt
    28501 aaaatggtgc tttttcctgg ctactttgga aattcagaaa ttactcctca caatatgcaa
    28561 tattatattg gttcagctat tgacgggatt gcccgaaaac ttactgaaca gataaaacgt
    28621 ggctattgtt tttcatgttc ggaaaatgaa actatcgatt gcatagaatg cgagcatcgt
    28681 tcacggaaga taatgtttca atttctggat atgttacccg aaatacgtcg catgctggca
    28741 atggatgtgc aggcagcgta tgagggagat ccagcagcaa aaagtcatgg tgagacaata
    28801 ttttgctatc ccagtataac cgccatgata cattacagga tagcccatgc actctatata
    28861 ctggatgtcc cgcttatccc gcgtataatc accgagatgg ctcactcgca aaccgggata
    28921 gacatacatc ctggtgcaac aatcagcgaa aatttcttta tagatcacgg taccggtgtt
    28981 gttattggtg aaacaactat cattggtaat aatgttagaa tttatcaggg tgttacattg
    29041 ggggcaaaaa gttttccgct tgatgaaaat ggtaacccca tgaaaggaat tccccgccat
    29101 cctattgttg aagataatgt cattatttat tcaggagcaa caatattggg aagggttacc
    29161 attgggaagg gatcggtcat tggaggcaat gtgtggctta ccgaaagcgt tccacctaaa
    29221 acccatatat tgcaacaaaa gccatcagag atgatgtttg aacatggtgg tggtatttaa
    29281 tgtttgtttt ttttaccaat taaaagtccg atatgctctc tgcctttact ccagtgccaa
    29341 tcattgcggg cattaaaata taaaaaccat tgttctttat ccacaggctt tgcatcaaag
    29401 gcatatacat ggcttttcat aaatgatcca ttgatattgt ccggtttcag taacgggtct
    29461 ttcaataaag ttttaaatgt tttgccatcc tttgaagaat agacaagtat agccgaaccc
    29521 gaatgattga tagtataatc ccagtatatg ccattttgca aacctataaa tccatcatca
    29581 aatgctaaaa ctttaaatga cccacatccc agattgcacc atcgattata ttgcgaaggt
    29641 gatagaatgg gttcgctgca gcattcaaag gggccctccg ggctttgtgc ttttgccaat
    29701 ccaatatgta gcggttcatt aaagccacag tccattatat atgctaatgc agcgctatag
    29761 tagaggtaat atgtactctt agttttaaca acacaggggt tgcttattga atatccaaga
    29821 ttgttgtgat gccatggtaa tgatggtttt aaaataattt tttcattact ccatgtataa
    29881 caatcttttg aagagcgcat tgctatatat gagtaccatt gatgggggag ccatgagtac
    29941 cataaaataa agggtttagt tttttcatag tataaatagt acatgccatt atcataaagc
    30001 aaaaacggac gcatagctct gcgaagagtc tgctttttta attttgtata ggtaatccca
    30061 tccatagaaa tatagtggtg aatgccaagc aaagagtgag caaaaagatg ccatttccca
    30121 tcctttgatt catctggagt aataaatgat gggtcagcaa taattggcga tgggaaagga
    30181 tattttatga ttggattttt ttcatacaac gaccatttca aacttataaa ttcttttgtt
    30241 ttcataactg ttcctttcat tgtgaatgca ctgcaatttt ttaatgctgt atgttcgata
    30301 gttactatta ttggtaactt ttgtattcca tttagtcaag tgcatttatt ataataattg
    30361 tatattaaaa aaactgtcgc atatatttaa gacctgaata tgaaaagcct ttgacataat
    30421 gctggtgata tggtactatt aacacgtttt ttttgggaca ataatgatat gtattccatt
    30481 aatgtaaagg cgataaaaat tttaatgctt aactgcatta tagttttgat atcatgttca
    30541 gcacattcac ctggtgtgtt ccagtcgctg gaaggcacat ggaagcttgc tactaaaaat
    30601 atagagtatg attattccaa taaaaggttg cctgaatatg tggaaattaa tattccctgc
    30661 tcaataaaag tgccccatac atttgaaaat aacaatatgc agttgcctgt taaaggatat
    30721 gtatggattc aaaaagaaat cttcattgaa tctttgccag ctaatgattt ggcattgcag
    30781 tttggtgaaa tcatgaatgc tgatgttgct tacttcaatg ggaaacaaat aggggcgtca
    30841 ggacggttcc cgccggattt taaatctgca tggggtaaat tccgtcatta tactataccc
    30901 agagacatta taaacaaagg cgctaatgtt attgcactaa aagtttacta tgattcagaa
    30961 ttctggatca cccctcctgt caggttgatt gacaatgttg cagggagtaa aattgccagc
    31021 ttgttggatt atttaagaat ccaattgatg gaaaatatat tcattttgtt aatactatta
    31081 tcattttttt ttctcatgat gttttacttc cagcgggagt acatggaata tttattattt
    31141 ggcatagcgt gttttaccat tgctatgcca caggcacttc attttttaga aaatttgtat
    31201 cctgatattg ggttgacgtc tgatacaatt ctgaagctaa cacagcctgg attgatatta
    31261 ttcccggctt tccttaatct gttttaccgg tatcacatcg ggaaacaaaa aaaatggtta
    31321 actatagttt atattgctgt gccgatagta ttcagcacta ttatggtgct gcagcaaaca
    31381 agggcaggaa tattattgta tagaaatcta tcgatcatag tatccattgt ttatatgata
    31441 gatttgttta tattatcagg atggcaggtg ttgcataaaa agaaggcggg agcgctcatc
    31501 tttattggtt tagtgcccat ggtagttctt ggaattcatg atgttttagc atttggactg
    31561 cacattatac cttcgtatat agtgttatat gtttacggat tgccattttt gatatttatc
    31621 attgcaattt atttagtaaa tatttttgta aaagctttaa aagaagcaaa agatttaaat
    31681 atacagttac agaagctatt gcttgatgca aaacgattgg cgtatctgga aaaggagctg
    31741 gagatagcaa aagctataca aagtcaattg ttaccatcaa aaattccaaa tatagaaggt
    31801 attgatattg ctattaagtt tcaaccggct atgaaaattg gaggagatat ctatactata
    31861 tttcagatca aggatggcgt agtgttcttt gttgccgatg ttgttggaca cggcatcccg
    31921 gcggcattga tttcagcaat ggttaatgtg ctgcttaaaa tgtttcagat gcttgctata
    31981 gatcctgctc ttttgctaag aagcttaaac gtaaatatat gcaccatgct ccctcaaaca
    32041 tttgtaactg cggtatgtgt ttattgtaac cgatcacata attttatgcg catagcgcgt
    32101 gccggacatc ctcctatgat aaagtgcagt aatgatggga atatttctat gtatatgcca
    32161 tcagggagag cattaggtat acaaacacaa acgcgattta aaactgttga attaacaatg
    32221 gagaaagggg acagattaat tatttatact gatggtctgg ttgaagcatc gttaccggga
    32281 aatagcaagg atatatatgg tgaacaaaga ttaaaaaata tgatacaaca aaccataaac
    32341 cttcctctgg ataaagctat tgaggtagta tataatgatg tcatatcata tgttggttcc
    32401 cctcagtatc ttaccgatga cattacagtc atcatgatag aaatactgta aatactgagt
    32461 gtcggctaaa tcaatagtta gtcattgatg ttgcaaattt gacacggtgt ggcaataatc
    32521 aaaaaaaatc tattatcagt tactatcgcc aaaaacaaaa accctgaatt ttccagcatt
    32581 tccatttgta aaaaatattt taagttggca tgataattgc ttgtataata aatagaacgt
    32641 tggaggagca taaccatgat gaatatacca attttacagt tttttgctct tttctgggta
    32701 tgtgtgcttc tttgggttat tattgaatat attgcttatc gtagtcataa gagagggcat
    32761 taatcaaagc atatccgata ggatacaatc cgtttattca taacaatcaa tcccagtttt
    32821 tatttttgac tgcagcttta gtagctgcag ttttttttta aaggtgttgc ctgtatcccg
    32881 tttgtacaac tcatggtttg gctggccacc aatcatggga tgaaaatgcg gtgtgtgcgt
    32941 catcctgatc cgcgattcag gatatcaatt atatgtggat tctggaacat gtccagaatg
    33001 acagaaggtg gaaaaggaga ttcatgatca agcagggaat gaccatgagg aataattcta
    33061 ttttttttgg gaggggtagg ttaatcttca ttgtatacat attatagata tattgttatt
    33121 gacgaatgat ttaatacatt gtttagatta tactatctat acaaatgggt gtttttttta
    33181 taataagtta ttggagaata gtcttatgaa gaagatagta attggtattt ttttgcttct
    33241 actaacaaat agctatgctc aaagtttttt gtatgaactt tcagaaaata ttgataatta
    33301 taaaaataaa aatataacta tgattatgaa attaaaatac tatgatacat tatttcaggt
    33361 tattactttt tatgatgcaa aaaatcatga tatatcattt gattgtattg atgtaatgaa
    33421 acacccatgg tttttacagc aaaaaaataa tttgcatccc ggtatggaat ataatgttac
    33481 atttactgta aaaggtcttg gtaacatgaa agaaatcatt gctcagttac aatattttga
    33541 accagtgatt ttacaaaaaa ttccttgaga atacattgat gcaaagacaa tgagataggc
    33601 ctatttacta aaaaaacgaa aggcaagata gctgggcggc tatcctgcct tcccctgaat
    33661 gggtaaacgt atgcgcagtg ccttgagcac aaaaagtagt gatcgggagg gatttactct
    33721 tgtgccctta tcagttatta ttatcggtaa ggtttaataa aatcttaaat aaaaaatatt
    33781 ttagctatta aaaaactact tgacacagta tgtataaaac atgtatcttt tataaagaaa
    33841 aagaggttgc atttttatta ataaaaaaaa ttataagaag gagtgttaca atgaaaaatg
    33901 tattggcagt aatgagtgta tttgcatttg cattcatggt ggcgtgtagc agtacacctg
    33961 ctgtacaaca acctgctggt gatccaaatg tagcattggt taatgcacag aataagcagc
    34021 ttgaaagatt accggtaact ggttttgcct ataaaagttc agtagtacca aaacaggatt
    34081 gggataaatg ggttgaagct gctgctccgg ttgtaaaaga ggtgataaat aaattaccag
    34141 aagggtatgt gctacaggta acaggtcata ctgatgcttc aggaccagaa gaagcagtgg
    34201 ggaataaacc tggaaattta aagatttcaa ctgacagggc aaaagctgta tataatgctc
    34261 tgaaagcaaa aggcattgat tcacccagga tgacctacaa aggtgttggt tcatccgaac
    34321 ttttaccagg cgttgacccg cgtgatggcg ctaacagacg tgttaccttt aaggttgttc
    34381 caaagaaata acgttttaac catttatgtg tttataaatt actaaaaaaa tatagagcca
    34441 ggataaaact ggctctattt gtttatatta tttaattctg gtatgatagt tcatgaaagg
    34501 tgttgtttcc aaagtaatgg ggctttattg tacagtttac tgcgatgcaa tatatataaa
    34561 ttgtgttatg cgcggtaaat tgcgacaatt aattgaagca ggaagtaaaa aattttcaaa
    34621 tatcattgca gttggtgatg aagttgtaat aacaaaactt gatgatggta gcggtgtaat
    34681 agaagaaata ttaccacgga ggaattactt ttcacggaag gaaaaaggga aacacaaaaa
    34741 aatagatatg attgctgcaa actgcgattg tgtggtggca atgcagtcgt ttgcattgcc
    34801 aagattaaat ttgcggtttg tggataggat tgcagtacga tgtgttgagc agaatattcc
    34861 gctggtggta tgtattaata aatgtgatct ggcaaaaaaa aaggattatg aatatgttac
    34921 taactactat aaaagtactg atgttaccgt aatcatttcc agtgtcaata ccagctataa
    34981 tatacatgca ctacaagata ttttgcatca aaaaacttct gtaatagttg gatattcagg
    35041 tgttggtaaa acaagcatcc tgaatgctat gtatccacaa ttatcactgg tagtgggtga
    35101 ggtttctgga agcaccggca aggggaagca tacaacgact aatgtttcaa tgattatcat
    35161 tgacgaaaca acacgaatta ttgatactcc gggattgcgt gaatttggca ttatggatat
    35221 tgaaccacat gtattgagca aatatttcta tgaatttgat aactatgcgc ctaaatgttc
    35281 ttttaaaccg tgcacccatg accatgaacc tgattgtgaa ataaagcgtt tggtacaaaa
    35341 gggtattata tgtgaagaac gatatatttc gtatcgtaat attctctatt cattaaaaga
    35401 ttattatgca acgatgtact catgatagca ttagagcatg tacagctatt gttcaataat
    35461 cattatgtcc ttcaggatgt aaatctgttt gttcaacagg ggagcacgta tgttatcctt
    35521 ggtaaaaatg gcagtggtaa aactgtgctg ttaaaaacaa ttgccgggct gttcccccaa
    35581 ttttcaggaa ctatcgccat aaatggaata tcgcttcatg aatatacaca gttgcctatc
    35641 aatgaaaaga ttcataagaa tatcaccatg ggatatgtat tccagaaagg cgggttgttt
    35701 gactcgatga cggtttatga taatgttgca tttggtttgc aaagacgagc gagcactgaa
    35761 gatgaaataa aaaataaagt gctgtcagca ttggaatggg ttggattggc cggttcagaa
    35821 aataagcttc cttcacagct tagcggtggt atgcaaaaaa gggtagggct tgcacgagca
    35881 ttgtgtttgt cgcctgcaat tatattgtat gatgatccaa ctgctggtct tgatccggta
    35941 ctaacagatt ccatatccga acttattgtt gccacacaga ataaatatgc aatgacatct
    36001 attctagtta ctcaggatct tgcagttgcg gaaaaaacag ctcacaggat aggtttattg
    36061 cacaacggca tgattattgt tgattgcagt gcagatgaat tttttagtaa aacaaacccc
    36121 tatgcaaaac aatttataga tggcgatagc gaaggaccaa tagaaattat ataaacagcc
    36181 tggtgatact atactatgaa tgcacatcgt cgcaataatg taatttttga tgtaataagc
    36241 tcttcaactg ttgtttgatg aactatgaaa caatgaatgg aagtcattga tagttagatt
    36301 tatgaacaaa atatatttga cagcttgctc attaattgct acattgaata acaatggcgt
    36361 attatatttt tagccatatc attatgtatg gtttttattt ttttatgggg gtcaaaaaaa
    36421 tgcaaactga acgtacaaaa tctgatcttg actggaagaa tctccccttt ggatatacta
    36481 aaacagacta taatatcaga tattattatc aggatggtaa atggtctgca ggcgagttgt
    36541 catcagagga aactatacca attcatattg ctgctccagg acttcactat gggcaacagg
    36601 cttttgaggg gttaaaagta tttgaaacag ttgataagcg tattgtggca tttagacccg
    36661 aggaaaatgc cagacgtatg aaggttagct gcgaacgtat atgcattccg gaactgccga
    36721 tgggattgtt tatggaagca gtggaaaagg tagttgctac taatacaaaa tatattccgc
    36781 cgtatgggac aggtgccagt ttgtatgtac gcccggttat atatggtgcc ggtgcaaggg
    36841 tagggcttgg cccggcaagt gagtatgttt ttatagtatt tgctacacct gttggaccgt
    36901 attataaagg cggttttaag ccagtgaaag cattggtagt agaacaattt gacagagctg
    36961 ctcccaatgg tgttggtgat tgtaaggttg gaggaaacta tgctgctggt ttgcgtggcg
    37021 atgaatatgc taaaaaatta ggatttcctg ttccgctgta tcttgacccc aaagaaaaga
    37081 aatatgtaga tgaatttgga acatcaaact tcattgcgat caaaggtaat acctatctta
    37141 ctcctgaatc aagttcgata ctacccagta taaccaataa aagtttaatg gatattgctg
    37201 cgctgttagg gatgaatgtt gaaaaaagac caattgcgat tgatgaggta gagcattttg
    37261 atgaagttgg cgctgttgga acagcagcgg tagtatcacc ggtatgcagt attcattatc
    37321 gtgataaaat atttacattt tgcgatgagg ataaagctgg cccggtatta acaaggcttt
    37381 atgaatatct gactaaattg cagacaggtg aaataaaaga tacctttggc tggttgcatg
    37441 agattgtagt taagtaaagt cttttttaat cattctggag catagatagc atgaataatc
    37501 aagatatctg ctggatacaa aggtttaaac atctcatgca agcgtttctt caattagaaa
    37561 aagcaattca tcttgctaat cagcgttcat taacagaatt ggaggagcaa ggcttgatac
    37621 aaagttttga atatacgcat gagctggcat ggaatgtgtt taaagatttc cttgaagagc
    37681 agggtattca gaatttatat ggttcaaaag atacaaccag agaagccttc aggcgaggac
    37741 taattgagaa tggtgaaatc tggatggata tgataaaaag tagaaatctg acatcacata
    37801 cctatcatag tgaacttgct caggagataa taaagaggat caaagaaagt tattttgagg
    37861 agttgaaacg ttttatagaa aaaattaaat tactatataa caaccagata tcatgaattt
    37921 tggattaagc aatactataa tagaacaaat ttgttctgtt ttttctaaac atcccgttgt
    37981 ggaaaaagtt attatatatg gttccagggc aaagggtaat tataaaaatg gttcagatat
    38041 tgatattgcc cttattggcg gaaaagatct tacggttgat gtcatgttta ttattataga
    38101 agaaattgat gatctttttt taccctatac atttgatata tcattattac gatatattaa
    38161 tgatcatgat gtgctggatc atatcaaaag agttggtaaa gtattttaca aaaagaaata
    38221 aaaccatacc cgcaattttc ctacatttat tgaataaaaa tagattgtgt gctatagtct
    38281 ctaatagatt ctaatagaaa taattgtacc cggaattata tatgctaagt aaacagcaat
    38341 atgccttatt gcttgacact tttaaatcag cccaaaatgc aaaaaacagt gatgcttttc
    38401 tttcagatat tgttgattta ctttataatg aattaaaacc catttctgtt tctgtatata
    38461 cgctggttgg cgatgaacgt ttggtattgc atcatgaagc tggtaagaat tttgatgaac
    38521 tggttgttaa tatttataat ttttcgctta ttgtccgtaa ggcaattaaa aaaaagagcg
    38581 tattgttttt accaacatca aaaccatttc agaacgatga aataaacata tccgttgcga
    38641 ttattcctct tttgcattct gatatagtgg tggggatgat atgtctggtg ttcccattgg
    38701 aggaattgca gcatatacaa aatagcacac catttttcac actcataggt aaaatagcag
    38761 gcacaatacg atattatcaa acagggctta aagtagctaa agctgatact gctgaagagg
    38821 agtttgagca aaagataaca agcttaaggg ttttggcgca gggtgtggca catgaattta
    38881 ataatatact ggcggttatt aaaggatatg ctgaactttt gctgatgaat gaagctgttg
    38941 acgaatccgt aaaggaagca gttgctatca ttgataagca aaccatccgt ggctcttcac
    39001 tcattgacag gcttagtgta tttgttaccg gcaaagaaat ttcttttgag tattgttcaa
    39061 taaatgattt aatcaaagag atagtggatg tacaacgatt tacactggaa aaagaatcaa
    39121 ttgaatgtaa attaaaatta ggtaaagttc cggaagtatt tgtagatnnn nnnnnnnnnn
    39181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    39241 nnnnnnnnnn nnnnnnnnnn nnnnnnnaga atcaattgaa tgtaaattaa aattaggtaa
    39301 agttccggaa gtatttgtag atccacgaca gataaaagag gtgttgctca atttgattac
    39361 tcatgcgcgt tcagcaatca aacccaataa aaaaggaact gtaactattg aaacatcgtt
    39421 taacagtgat gcggtgatgt taaagtttgt caatgatggt gttccaggat gctgggaaaa
    39481 tggagatcct gataaaataa aagctaccaa tggagttgat agtgtaggta atggaataag
    39541 tttagctatt gcatttggca ttatgaaaag ccatggtggg gatatgacac tatgtcatga
    39601 tccagcaaaa tgtaatgagg taaccatata ttttaaaaaa tttaatcctt catttgctga
    39661 tgctgaaaaa aaagaagaaa aagagatgct atatttaggt gaagtcagga tattgatagt
    39721 agatgatgag gagccaattc gacaattttt gtcttcagct tttgaacgta tggggtacca
    39781 ggttttaaca accgataatg ggcaggaggc tattgatatt tgcacatttg aggatatcga
    39841 tattgtattt ttagattatt tgatgccagg ctataaaggt gatgcagtat ttaaatcaat
    39901 acatgatgta agccctgcca cggatgttgt atttattaca ggagttgatt caatacctga
    39961 tatagacaaa ctattgatga tgggattaga gggtgttctc agaaaaccgt tcaagataga
    40021 tacaatcatt aaacttacca atgagattat ggcacgtaga tacaatactt tacaggaata
    40081 aagctaattc tattctgatt tgataactcg ttctgcctgt acagctaaat atttgaccaa
    40141 tgtagattcg ttgatatatc ctaaattcat cagtattata ccaataagcc ctccatcacg
    40201 tttctggata gcaagagctt cattaagctg ctccttggtt atcacagcat cttctacaag
    40261 tatttcaccc agtaatggtt tagccatagc gcctccttgc aatagtaaat gttgatgttt
    40321 gttacattcg atgcaacagt aatatgtgat tataacaatc gcctgacagc attcttccac
    40381 aaattaatct cacgctgcct ggttgtttca ttcattgatg ggttatatcg ttttgcaatt
    40441 ttattaaatt ttgctacttc atcgatagtg ttataaacct tgccggtaat acctgctaaa
    40501 tatgctgctc ccaatgctgt tgattcagta atttcgggta gcaaaaccgg tatacccagt
    40561 atgtcagact gatattgcat caggtatgtg tcacgggttg ctcccccatc cactctgagt
    40621 tcatggatgt tttttcctga atcgctttgc atagcttcaa gtaattcata cgattgtaaa
    40681 gcagtggact taaccgcagc acgaacaatc tgttcaggtg ttgtatcacg ggttaagcca
    40741 aagattgcac cgcgtgcatc cattttccaa taaggtgcac ccaatccaac aaatgccggg
    40801 acaaatacaa cctcatcttc tttatttact gaaagtgaaa ccttctcagc atcggagctt
    40861 ttttcaatga atttcatata atcacgaagc cattggacta cagcaccgcc aataaatact
    40921 gagccctcaa atgcaaagac tggctgcccc ttgttgtcac aggttaatgt ggtaaccagg
    40981 ccgtgttttg acagtatcca ttcattcccc atgttgagaa gcataaaaca gccggtacca
    41041 taggtatttt ttgaagttcc ttcaaatacg cagttttgtc ctaccattgc tgcctgctga
    41101 tcaccggcta tgccacctat ggtaattgca tcaggcaatc cgggcactcc ctgtgtttcg
    41161 ccaaaatatg aggcagattc cctcacttca ggcaaaattg tgcgcggtac gttgagtatt
    41221 tcaaggagct ccaggtccca gcttttgttt ttgatattaa acatcaatgt tcgtgatgca
    41281 ttggtataat cggttttgtg tgattttcca ccggtaagtt tccataaaat ccatgtgtcg
    41341 atagttccaa ataacaattt gccacttttt gctaaatcac gagccccctt aacattatca
    41401 agcatccact ttactttggt gcccgaaaaa tatgcgtcaa tgacaagccc tgttttttct
    41461 ctgaatagtt cttcgtagcc agcttcttta agctgattgc atatatcggt agttctgcgg
    41521 cactgccata cgatagcatt gtagattggt ttgccggttt cttcattcca taaaactgaa
    41581 gtttcccgtt gatttgttat tccaatagca atagcatcat ttggatttaa atttcctttt
    41641 gcaatagcat cagggataag ttgtgcaacc gaatcccata tctccatggc attgtgttca
    41701 acccagcctg gttttggaaa atattgtgta aattcagcat aactgctgga gatgggaagc
    41761 ccatgttcat caaagcagaa tacacggttc cctgtagtgc ctaaatcaat tgcaataaga
    41821 aatttcttgc ccatagcgat ctccaatggt attttcttag acgatagttc atggtatgta
    41881 atcattgttt aaaaaaagct ataatgcaag taataaattt tatagctgat agcttgtttc
    41941 aacgatggtt aatcatcatg agattgatac aaccatagag tatagagatt tgaattataa
    42001 aatcaaaatt tgtcaataat aaaattatgt gagaataaaa actcttatta taaacattaa
    42061 tgccaatcac aataaagtag ttgacaatac atggaaacat tgtacagaaa ataaaacacg
    42121 tgctaatttc atatctatgg caaatgacgt tgtatacctg taaatctcaa tgatagtatt
    42181 atttttttat cacttttgta ggggaacttc tcacgggcat attctgaatg catcattgtg
    42241 aacatgagca caagcagggt atagcagttt attagtttaa tttttaaata ataaaaaata
    42301 tgtgtaacgg tatcaataaa aagtagacaa ataaaacatg atggcaaagc tcattcatgc
    42361 tattgtaaaa caaatattaa tgaatgaaat attcatttat tgtataaaac ttagagaaag
    42421 atgaaataca gtgtgttatg gtatggaaac taattttcac aacagatatt tagaaaaaat
    42481 agctcaaaaa aagcctgttg tcattgctga gattggactc aatcataatg gcaaccttaa
    42541 tgaagcaatt gaactcattc accgggcagc agattccggt gcggatttag tcaaatttca
    42601 gataatcaat gccgaactgt
//
LOCUS       DAOOJH010000015        41272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1062_length_41272_cov_9.429895, whole genome shotgun sequence.
ACCESSION   DAOOJH010000015 DAOOJH010000000
VERSION     DAOOJH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 41272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41272
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1062_length_41272_cov_9.429895"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLJ75_03780"
     CDS             <1..695
                     /locus_tag="PLJ75_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HPD04368.1"
                     /translation="RAIQRLIGMDIPLIMVSSKTHSEIMKLHGALGLHHPFVFENGAG
                     IGFADGSYILLGKDIQELSNHKTIIRAMVKNCIWVDDIPVQELSLYTGLSHDDVTLML
                     QRKASLLFMAEQLPTTITDINSSIAEYGIAITTGGKFFTVQDSRVDKGRAVQYIKNLF
                     QNRYNDMYTIGIGDGYNDIDMFNAVDEAYFVGDDTLFISIQGQCNKVTMTQRKGPDGF
                     SDIINMITQPNR"
     gene            complement(685..825)
                     /locus_tag="PLJ75_03785"
     CDS             complement(685..825)
                     /locus_tag="PLJ75_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04369.1"
                     /translation="MAHYKLDGAKFETLDELKEVMWQLYKDKMTREEFDSYVEQHVQV
                     SD"
     gene            987..4178
                     /locus_tag="PLJ75_03790"
     CDS             987..4178
                     /locus_tag="PLJ75_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HPD04370.1"
                     /translation="MKRYITINILLLVFLCAIVTGGLCQTLYIDDTFTFSEAGLHLDY
                     LTGVDPSTGIDEIVKRYNNHEFKPSPSVHPSFGYSTQCLWLSLTVTNTTSLPVQWLLE
                     YDYPLIDDITLYIPHDHSFTAIQTGDRLPFSHRQIPYRTFVFPIVQKPGTTTYFMRVQ
                     SQGSLVVALKAWTPHAFEEMRNKTLPFIWMHYGLMIALFFYNLFLFITVRDKSFLYLV
                     IFIIGVTLFAMANNGIAAQYLWPESPWWGNVCHPFFIFLAMVGVVLFTREFLNLPVVI
                     PVLGIVLLIMAIVTAMGGIIPFVAPYYYATQISVLMAAIEAIIAIIIGIVAQIKKVKE
                     ALYFNVATFSFVAGVILIVLRSFGMLGDNFFTAWGYQLGSSAMVILFSLGIADKVNTW
                     RKDKERALSQLATSEAKYRTLVENAHDGIMLVQNWNMVYSNNALARMLGYTVEELLNK
                     NINFITADSSLGQEVIIQYENRMLGKAAKLQYEAQLKRKTGTVMDVLISAQKVILDGI
                     STSIAIITDISSLKEAHYTIQQQYEEIQSQYEELEAMNEEMVRTHYELTEATHNLEEE
                     KERLHATLRSIYDAVIATDIQGKIIIINARAEQLLGNISTEVIGNQFVTVFPLRYTTM
                     EEFKNPVNEILRYGSLHTAGEPLVLQLHTKEIIVELSGAPIYVEGKIIGTVIVLHDIT
                     QQYWMEKEMHKISKLESLSILAGGIAHDFNNLLTAIIGNLSILGMKLSENTVYQKYIE
                     RIENAAQRAVDLTKQLLTFSKGGEPIKAVASLEQLITDTAQFVFSGSNVRCHLDIQPE
                     LWNCEIDAGQMGQVFQNIMINAMQSMPQGGIVTIKAYNYVYDGKAQIPLLPGDYIAIT
                     IKDEGIGIPEENLSKIFDPFFTTKEHGSGLGLATSYSIIKRHGGYIEVTSDVGKGSEF
                     IIYLHATHETNEEKKEGSAIINSYEGKILLMDDDFTIYEVVSHMLDMYGFTVDWVESG
                     EKAIAQYKKSLDEKRPYDIIIMDLTVPGAMGGAQATKEILRINPDAKVIVSSGYSNDP
                     IMSHYKEYGFKGVIAKPYRIEEIMNVINDLMKQ"
     gene            4320..5012
                     /locus_tag="PLJ75_03795"
     CDS             4320..5012
                     /locus_tag="PLJ75_03795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04371.1"
                     /translation="MKKQCIVFIGIAALLTINASVVKQKQSDSAKVKEQYSNDIKRKE
                     DIQKKIYDLMKEINAHAGGDALIVPVDPEKADNVVDHDDYQKKEAGEKKADDKKTDDT
                     ALIAPTKGKTTSKYGYRQNPVTKKYAFHKGIDIAAPKGTKVVASAGGVVATSNYLNNG
                     YGNCVIIEHANNVKTLYAHLAQRTVAAGDTVIQGQKIGTVGATGRATGPHLHYEVYKG
                     GKSINPEDLVHY"
     gene            5030..5668
                     /locus_tag="PLJ75_03800"
     CDS             5030..5668
                     /locus_tag="PLJ75_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPD04372.1"
                     /translation="MNKKACILGATGLVGGELLSLLLHDDYYAHITIIVRKKIAIPSQ
                     KVAVIVTDFANLEDASEAFQVDDVFCCLGTTMKKAGSKDAFKQVDYEYVLKAAAIAHK
                     QGAQQFLVISAVGANPHAYAFYNRIKGLMKKDVSSIPLQTIHIFRPSLLVGKRQEKRL
                     LEDIARLFAKPMQFLLKGSFAKYAPVEARTVAEAMAGAAKLNTKGVHIHEAL"
     gene            5914..6804
                     /locus_tag="PLJ75_03805"
     CDS             5914..6804
                     /locus_tag="PLJ75_03805"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HPD04373.1"
                     /translation="MTGNDIKTVLIIGSGTMGQQIGFVCALHGCHVHMYDVHEEALKS
                     AQNRLTQLAQWFEKHTVKNTTNALSLIRYYTDPQQAAIDVDFVSESVPENPELKAKVF
                     STFHALCREQTIFTTNTSSLVPSQIAAATGRPDRFLAFHFHDIRTTKIVDVMPHAGTS
                     QEVINATVEFAKKIGQIPIVLKKENNGYVFNTMLMTLLASALTLASNGVADIHDIDKS
                     FMGVMGTPIGPFGIMDSIGLDTVHEITKYWADLLDDKQGKKNAALLQQYVEKGLLGTK
                     TNKGFYTYPHPEFSKPDFLQ"
     gene            7006..8139
                     /gene="tgt"
                     /locus_tag="PLJ75_03810"
     CDS             7006..8139
                     /gene="tgt"
                     /locus_tag="PLJ75_03810"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD04374.1"
                     /translation="MRFEILQHDSNCNARLGRIHTPHGMVHTPVFMPVATRGAIRALT
                     FRDIEELGFEIVLSNTYHMYIRPGTNLLQKAGGLHNFMNYHKPLLTDSGGFQVFSLAD
                     LRKVHQDGVEFQSHIDGSKHFFTPEKVLDIQRIIGSDIMMVLDECVAYPADYQTAKTA
                     VDRTVQWAGRSYEYWHNNFDIEAQALFAIVQGGVYPDLRLQCSTELQQYNFSGFAVGG
                     LSVGEPKSQYSDITAVTVPALPWDKPRYMMGVGSPLEILEAIRYGVDMFDCVMPTRIA
                     RNGTLFTTNGRINIKSGIYAEDFNPLDEHCHCYVCKTFTRAYLRHIYKMNEIASLIYN
                     TYHNLYFMKYFMDTIQKSIANNQFMDEYNRWKKIYNNSEINTM"
     gene            8322..8663
                     /locus_tag="PLJ75_03815"
     CDS             8322..8663
                     /locus_tag="PLJ75_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04375.1"
                     /translation="MDITKRTRGDVVILDISGEIDLYNAPEIKDIINGLIEEKKFNVI
                     INLEKVTYIDSSGIGALISSLSNLKKYQGGLKIINVFASVRKVFELTKLTSFFEIYDS
                     EEVAIESFNKR"
     gene            8921..10246
                     /locus_tag="PLJ75_03820"
     CDS             8921..10246
                     /locus_tag="PLJ75_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HPD04376.1"
                     /translation="MRSQYHISVIALLSALVLMTACFEKLPVKELSLAKNAISDAEAV
                     KAPYYAKDEYNQAKDLLVAAHDNVKNEEYDKAQQNAIEAQKKAREAYDKAIPLLAKDT
                     LDIATKSVEAADNVYAGVLAKDDFAKANEMLQEANKNYENKNYLATYKAALDADMYAK
                     NARNVALSQKETLADSIKEVNIIIAQAESYNANQYAPEKLKLAKEQSQVARSAYDAEE
                     LKKGFSAIEVAKLNADEAYFASLKATAAKKIDEAQGAIVAAKATEQAKQSPDDIKAAE
                     ESLSNAKTLFNDAKYKESIIASGEAITIANSVSAKKVAVAPVIESEVTPEGKKEVQSE
                     TQKEALQKKVEEETEYTMYKVKYNPEKRDCLWRIAVKFYNDGFKWKLIYEANKDLVKD
                     PNLIKPGWVLKIPKTATKLAQPKKEGVNEQTAVKEDEAPTTELKMKTQE"
     gene            complement(10285..11061)
                     /gene="mazG"
                     /locus_tag="PLJ75_03825"
     CDS             complement(10285..11061)
                     /gene="mazG"
                     /locus_tag="PLJ75_03825"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPD04377.1"
                     /translation="MSQSPLYHLKEIAGILRSEHGCAWDKAQTSDTLKSYLIEEAYEV
                     YQAIEHGDIDNLKEELGDLLYQVYAHAQIASEHDLFDIDDIANSIIQKLIRRHPHVFG
                     NETANDKYEVINRWEKIKKLEKNSGQSLLDGVPYHLPALLKAYRVQQKVARVGFDWEK
                     LHDIIAKLNEEVNEFAVELQKNKENKEALKDEIGDIFFTIVNIARHLEINPEEALQHT
                     IAKFTKRFHYIEQRLNEMNKDIAQTPLSELDALWDESKQK"
     gene            11306..12568
                     /gene="glyA"
                     /locus_tag="PLJ75_03830"
     CDS             11306..12568
                     /gene="glyA"
                     /locus_tag="PLJ75_03830"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPD04378.1"
                     /translation="MALHKFLKDADPEVFSILQKENMRQENSLILIASENYTSQAVLE
                     ASSCTMTNKYAEGYPGKRYYNGCQFVDEMERLAIERIKKIFNADVANVQPHSGTQANM
                     AVLMALLEPGNTIMGMNLSHGGHLSHGSPVNFSGIYFKVVSYGVTKDTQRINYDEAYS
                     VAKEHKPKIIIAGASAYPRTIDWNKFREIADEVGAVLLTDIAHIAGLVATGHHPSPVP
                     VADFVTMTTHKTLRGPRGGIILTKQKYEGMMNKYIFPGIQGGPLMNTIAAKAVAFGEA
                     LQPSFKDYSAKVIANAQALARTLLSRGLHLVSGGTDNHLMLVDLHNKNLTGKDVANRL
                     EEAGITCNKNGVPFDDKSPMITSGIRLGSPALTTRGLNEDDFKHIGNLICDIIDNMND
                     DKVLQRVQAQVQELCKHYPTDFLRLEPR"
     gene            12638..13627
                     /locus_tag="PLJ75_03835"
     CDS             12638..13627
                     /locus_tag="PLJ75_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPD04379.1"
                     /translation="MKRLIGVIMICLIAVVGCKKEKKYTVAREGLVNLISGDVVLVVN
                     GAESKAQIGDAIKEGMIIKTVGPKSIAEIYFGENAVKILGDTVIKIEKLITDISANTE
                     ASSFVVEKGRMFSRVTKKLNKGDSYQVKTPTAIAAVRGTDFLVTQEEDKSNVACLDGL
                     LEVLNQSLATGQPIVLEEKQEVDIVPGQDMVKKQISEDKLRMLNILLEIQAMREDIRK
                     KYEQQKEEILKYVEEQKEANKTMLEEQREKDKALVEEQKARDKAMIEGIKGQIKDESG
                     KAAQQSLDAAKSQMQDAKAVDKDQASSEAKDQMSAMKPKIEKQKIDLNQFKTQ"
     gene            complement(13963..14433)
                     /locus_tag="PLJ75_03840"
     CDS             complement(13963..14433)
                     /locus_tag="PLJ75_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HPD04380.1"
                     /translation="MKRIHFISLLLIVSLSGCGLEDLLHKIAFKLSYYMAKSKLEKQF
                     EEEQGIILAPPPSLSKFDIAPIDTKTNDKKPHFLRITMSLGYEENEDLESELKQRKDA
                     INHICRILIKDKSSEEVNSVEDTVNLAEEIKAHINSLLTKGKIKEVYFKEFVVK"
     gene            14763..15548
                     /locus_tag="PLJ75_03845"
     CDS             14763..15548
                     /locus_tag="PLJ75_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD04381.1"
                     /translation="MIQLAFIGTGTCNATARKPQALAFSIDGELVCVDFGGGAYHAIA
                     SLNNPDFNYGNISTVVLTHYHVDHISGLPDLLWGEMWDYTGHRTEPLTFVGPKGLKNF
                     YEDRLLPFMGDYPLPFKVELVELHDGDIYKGNGFTLQSNTLAHGDFSSGYLFDCGSKK
                     IAITGDTGYCDALQRLLSVADMAVIEWGIASDDTYPLHVSSKDIISLVNAGIWPQQVY
                     AVHVYPLPDLDFETQLQTMRSVVASHNKSIEFPSDGDIITIIP"
     gene            15657..>16489
                     /locus_tag="PLJ75_03850"
     CDS             15657..>16489
                     /locus_tag="PLJ75_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD04382.1"
                     /translation="MKAIKVDYCIIGAGVVGLAIARELSLRTKKTIVVCEKEAQAGFH
                     ASGRNSGVLHAGIYYTQGSLKANTCIEGNRLMKEFCAEKGVAVKESGKVIVAKNQHEV
                     PTLYELEQRAKNNGVTVELINEKKLEQIEPCARTNTLALFSPHTAVVDPKVVMQALYH
                     DCIQRGITVLLSTPFKQFTSDTSIIAGNHTITYDKLINCAGAHSDTIAHTKALAKHLA
                     LIPFKGIYIRLKPDSKPAINGNIYPVPDIRNPFLGVHFTRDPYGNVYIGPTAMPVFGR
                     E"
     assembly_gap    16490..16589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16590..17440
                     /locus_tag="PLJ75_03855"
     CDS             <16590..17440
                     /locus_tag="PLJ75_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPD04383.1"
                     /translation="EDFMRPDRVIIGTDDEEALAVMKELYAPFTHSHERFIAMDIRSA
                     EMTKYTANAMLATKISFMNEIANICERVGADVNMVRIGIGSDHRIGYSFIYPGVGYGG
                     SCFPKDVQALIKTAKDYGYEPKVITAVEEVNNKQKLVLVDKVVQRFGEDLTGKIFALW
                     GLAFKPETDDMREAASITIVKELVKRNAYVKAYDPKATEQAKQCYLHDIKNIEYATSK
                     YDALNDAEALLLVTEWKDFRSPDFDEIKKRLKNPVIFDGRNQYNIKKLQKDGFEVYQI
                     GVGNVR"
     gene            17430..18395
                     /locus_tag="PLJ75_03860"
     CDS             17430..18395
                     /locus_tag="PLJ75_03860"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD04384.1"
                     /translation="MSDKTKFFITGSSGFIGTYFIQFLDNSNINAEVLGVDIVPSPLT
                     GTKAIVFHEEMCTMNDSNAVQLLLQKHKPDYIIHFASQSSVAKSWDDIPATLHNNVNS
                     FILLAEGVRKYCSDTRMLVIGSSEEYGEGAPDNLPIEETCPLHPQNPYAIARVAQEML
                     AIMYVKSFRLDIVMTRSFNHIGPGQSEHFAIPSFVKQIITAKNNNTSCILKTGNIDVV
                     RDFVDVRDAVKAYYLLLKQGKTGDVYNICSGVGYSLRAIITMLSEITSVQVVIEHDQS
                     KMRPAEVQTIIGSYQKINTLTGWSPTINIRQSLEDIVKGQSSALH"
     gene            complement(18392..18577)
                     /locus_tag="PLJ75_03865"
     CDS             complement(18392..18577)
                     /locus_tag="PLJ75_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04385.1"
                     /translation="MKRLVFFFIIATVIFASHSGFAANTISEVPIRIFFELVPALRIA
                     PDVDFGIRGGLGARYFF"
     gene            18982..19431
                     /locus_tag="PLJ75_03870"
     CDS             18982..19431
                     /locus_tag="PLJ75_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HPD04386.1"
                     /translation="MRLRQTLLKILINFYPPFLGAGIRIRFDKKNKKVYVSMKLTWYN
                     RNYVGTHFGGSIYAMCDPFYMFLLMEILGSNYIVWDKKATIDFIKPGKSTLHAEFFID
                     EQTVEAIKTEVEQKGKALPQFTVAVKDDDGEIIARVDKYIYVKKKKT"
     gene            19428..20384
                     /locus_tag="PLJ75_03875"
     CDS             19428..20384
                     /locus_tag="PLJ75_03875"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPD04387.1"
                     /translation="MIYPATDITIQQAAEAIKQGEVVAFPTETVYGLGANAFDRLAVA
                     SIFAIKERPHFDPLIVHVNSLSMVSMVAAAIPCAAMKCMEKFWPGPLTIVLPKKDDIP
                     DIVTAGLATVAIRMPLHPVALRLIELAGVPVAAPSANPFGYLSPTTAQHVAMQLGNKV
                     AIILDGGKCAVGVESTIIKIGNDEINVLRYGGIPVEEIERTTGLVVTSHTTTIEAPGQ
                     LPYHYAPQTKIVLVSDIMSVPDDATSGLLLFKKRDIPYTSIHIMYLSDEGDMRQAAGN
                     LFSCLHALDAMKLKVIYAEQVEEVGLGRAIMDRLKKAQARFM"
     gene            20688..22079
                     /locus_tag="PLJ75_03880"
     CDS             20688..22079
                     /locus_tag="PLJ75_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPD04388.1"
                     /translation="MIRFDYNYFMDEHIGTQHGLSQNDIDGIRPEFNEVLKRMDDEKA
                     EGLLGFLEIPFNTSLVKDIQSFRQHTDWCDTIALIGIGGSALGPQALKNALADIYWNE
                     LPDEKRGKRCKVYFFDNVDPIEMNSLLDIINIPTTLFLVISKSGGTTETNANFAILLD
                     TIKKNTRDYKKHIVTITDPAKGIMRQITREEGFTSFPIPANVGGRFSVLTAVGLVMAE
                     FIGIDINGLLHGAAEVIDRYYGKEVWSNAPLLNAAFHYCFDVKKNKRINVLMPYTRKL
                     FLFADWFRQLWAESLGKRFDIYGNEVMVGLTPVAALGTVDQHSQLQLYLEGPNDKVIT
                     FLKLQDFGTDITIPEFYKGKAELDYLSGKSLRKLNGFEEQATELVLKEASRPNASIIL
                     PELDERSAGQLIMFLEMQTAYAGYLYNINPYDQPAVEQGKRFTFGLLHRPGYDEYEQK
                     FKSGYQKKENYIL"
     gene            22076..22690
                     /gene="tmk"
                     /locus_tag="PLJ75_03885"
     CDS             22076..22690
                     /gene="tmk"
                     /locus_tag="PLJ75_03885"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPD04389.1"
                     /translation="MKIMTPLLIVFEGIDGAGKSTQATLLYAFCRECAKTALLQEPTD
                     SRWGKSIRKILQGELQATTQQMLDLFLLDRAYDVEHNIKPLLHKGYVVIVDRYFYSTA
                     AYQAGDGNSPHEIVRMNFEKGFPVPDRVYFIDIEPEIALTRIAMRSGNNRDIFEKLSE
                     LTRIRSNYLSIADDRFCIINGNNSKEAIFDAIQKDFIQHFSDVT"
     gene            22698..23993
                     /locus_tag="PLJ75_03890"
     CDS             22698..23993
                     /locus_tag="PLJ75_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPD04390.1"
                     /translation="MKLLRQLIIQCFNVCFIAILLLMGWMLYNVHIAGDLLIIFLIIV
                     TVTVFPLYTYWIEHIHKKILPFRYEDLYLQTVDMFLSLQSYDDIIKVIFERVLRFIGV
                     RSGLLIFYSHATDDYTIYYQKQQKQKIIRKARIDKNNIILKIIQSGDDILIKSKLDPS
                     LHFQRSIIFEMEKLGSEIIIPIYYHDIFLGLMVIGEKKTRISRKDILMLKALASKIAT
                     VTVNSFFMHELIKNKEIEKEYELSFKVLKKFLPPDEGFIHNTHYRVIRHPGNEAHYFF
                     SIYNCSDSISYVIICSLRANIPLLSLLIPAISVLFQSYARLGYYPLTIIEKINAVIKK
                     KELIEKDLSLLVLKKCNNNYFIGKSRTSDIKLFVYNGSTVKPVMSNDDSAMIRNPVRL
                     IIARHDMAAIKKNPELLDNTVSLSNTIGENEFIMILTRA"
     gene            23993..26305
                     /locus_tag="PLJ75_03895"
     CDS             23993..26305
                     /locus_tag="PLJ75_03895"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPD04391.1"
                     /translation="MKKHYRIYACIVFYIVFFILIVPQSYHNALQYTFDRLVIGKTFY
                     YPISSFPKAMFQYHIISCRQLATTSNDDAGLECTMAGNNEMLQQQFTNNNRTAVWIVL
                     IVSISSIVIVLWGIGVYLARHDIFSQLFMIVAVSIALCLLMYIDVFFTGKLFIMFPVV
                     NILVGWSLLHVTYTINDYSKHGKKVWYTAAACIALVLSIISLVSDIAFETIYMLTIIM
                     TLLLFLASIMVLTVTINHKLSFVLRRNLVIAAAIFLGGVVPYAILLTGTIADMYPGIL
                     FALFFAIATPVSAGNGLLASSSLASMANLQKNFIAMVMDIIIAVVLAFSLFYIYNFYS
                     ILSGMYYGIIIFVIAMIILLHLRMLIVRTFDDIIFLNNDEYALSLQRIAEIITSPRSL
                     KDKLDSIQSELHTLIRVEGIQYAIIDDYEYGDSRIINVKSDSDIVTFFKKRKETITMT
                     SSFTNNEYERALQRYCKHDHISSCIPVYIDGILQGVLLIKGKEGDYFTNPELSFLAML
                     SLQVHQLIVNSKALAEYIASRDYETEMDLASYIQLRLFPKKSPVDCGIQFAIYARPYL
                     KVTGDYYDIVTIDKNKVAVVIADISGHGLSAAMILSATSAIINGMLKEKKNISNVVAE
                     LNHFLTMRYQGFELITLFIGLFNKKTRSMEYINAGHIAPVVLSHDKKFYRLEGRSKIL
                     GVDPAAQFHASRYSFKAGDEMILYTDGLTDLYDARNDVSFGDEGLEEVLQQTIMKPIE
                     EKIHAITEAIEAFGQEHIKDDITIVGMYID"
     gene            26223..28721
                     /gene="sppA"
                     /locus_tag="PLJ75_03900"
     CDS             26223..28721
                     /gene="sppA"
                     /locus_tag="PLJ75_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPD04392.1"
                     /translation="MQLLKQLRLLDRNISKMILPSLACILISIFWSNLAYAEMKVQPS
                     DGVFPGVTASVPSLPAYSYSNPVYADINEGFSLQYRYAYIDPVGVGTHYVATTFYGFT
                     VGYNTLEIEIDDKEITRTDGNSWYFAKGIMLTDWFGFGILYNRYTFDGETVKAWGYSF
                     LLQPSKYLSFGCVIDHANEPSPGLLSLRQREWYSMSLRPFGNMFSLSWDIKRYEGEEW
                     GDVLHIFTLSGMMWHDILYAVQYDSDKNISFSLAVPFDISTRTGASSIYDFGYTNFNK
                     QESHAYTFGITLTDERFRNAIGPKQRFLSIVLQEPVKEIKERGIFERSRASFFEIVRS
                     LREATYDESIKGVVLQIDAPVLTFAQAQEIREEIKRCKGNGKPVYCIVTSVGNLEYYV
                     ASSATKIYYTPNQPFGITGLKAEVYFFKGLLDKVGIRFEEVKKGQYKSFGESFTRQHM
                     SDAARENLIEVLKDLNQQFISDIASDRKLTVEDVEKCMAGGILTPQQAQAQGFVDVVA
                     RPMEVLQSLGVERYGLSYMVQCDDYVEEKMYAYDWGPKPQIAVIYVEGNIIRGTAKDN
                     NFLSPAMIGDVNYKKFLEKVFADSSVKAVVIRINSGGGSAIASDLMWHSLVALKKRYN
                     KPVIISFGNMAASGGYYIACTGDTIFTSKSTITGSIGVLFGKLNLEELYAKLGISKET
                     IKMSEFADIFSESRAMTEKEKQLIQKNVDFTYNEFIQKVEMGRNIPADAIPQVAEGKI
                     FTGSQALNNGLADTQGGLIVALSYAASLANIKGEFKVIQLPEKDIDLRKILKGTSFQT
                     ALSDMMQYMYNMLIFESLNKEEVLLLYPYEIVIK"
     gene            28784..30265
                     /locus_tag="PLJ75_03905"
     CDS             28784..30265
                     /locus_tag="PLJ75_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04393.1"
                     /translation="MKRVSMKKLQLFLCMVFILASTVLYAKPKAGFGLIRGFSSEEMF
                     LATLLRQHIINIAASLQIFDSVNPENLARQQQRYACYDEQCMLLFTREMGLSLFVIGT
                     VSQTASGYRVHIAAYGLDIPYNGKEVCSYTATVNVTIMDKSDREASYIAEEIASQFIV
                     LLCQRLQVPAYISHNTVKSDFTINGTFTCYTVETLHDDFVSLKPMDNVTIVNNIVAGN
                     GVYDDCMVLIDFKKEAEAIQQFYYGRKQEIVFKKAAWDDSCSLIAFTIPASATMPVVV
                     PFFGYYASEDYEGLLLWIGNVVPYTGIQMWGFTHKPDTLKKHHEDISRHDRALYYFGW
                     YMLFVGGMPSFVDAFSHSYLEQARYYKGNVPTMGDKKNEVYLSLLGGGSGMFYKGYRA
                     WGYWYFHITNLCVYGLLYNYARPSEWDSANHRYRTHDANKSASYAFLSLLSVIKIVEM
                     VHTLSVPYTISVVEQEDARIEVLPVFAVNDYEKITGIACAMHF"
     gene            30247..31686
                     /locus_tag="PLJ75_03910"
     CDS             30247..31686
                     /locus_tag="PLJ75_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04394.1"
                     /translation="MRNAFLIVWCMVYSVIGTAAYADDIPIGVIIVNTSVDTPMIKAL
                     GSITSEAIKEAFKNGRHLVPYDAQTFLQITNTKSADEIVGKAHLYGVSLIVYMQVFLV
                     GPVYTCNLTFKPLEAMSTFKEETISIRSTIAKNIPLKAAKEILKRHSDVLPVTIKQKI
                     NNYTYIINAGQWHGLQQGRYTLTDGAECAIVSVKRYSALVTIHADYNEAQQITMSVGN
                     KITKLIKRIDAQIHENINQEYSGKKLLKGDSDGKRAIEATCVVNPSGNILLPGYGAFL
                     STHYLGFSHSEPDWPGIYVALGAVATQLMLVPALGNWDVNFFPWVNGNKTNAQYRLGI
                     YLWATLPVTYTVAFFNQLSYAYQKQKVLPPFFLHDDVTAFVASAIVPGGGLFYKGYRL
                     WGHAYWLTECTLGGLLTYHWEDGGKRAVFAGALIGIKLIELIHAVLASPAYSVYTYEL
                     SNNENVHVDVGMAPFQNEPCFYAYFFKNY"
     gene            31740..32825
                     /gene="carA"
                     /locus_tag="PLJ75_03915"
     CDS             31740..32825
                     /gene="carA"
                     /locus_tag="PLJ75_03915"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPD04395.1"
                     /translation="MQKRAYLLLEDGTIFEGYSFGYEKDAIGEAVFNTSMSGYQEVLT
                     DPSYSGQIVAMTYPMIGNYGVNNDDVESSKVQVAGFVVKEYSKAYSSHRAVRSLDEYL
                     NDYKIPGIEGIDTRRLTRHIRDKGAMRAGICMDTSGAMERILAHPAMAGLDLTCNVMC
                     NKPYSFGKDRSEAPTIAVFDFGVKMNILRLLHEQGCNVVVYPGNMALSQVLKDRDIKG
                     VFLSNGPGDPDAVEYAKILVKDIMKAHIPCFGICLGHQLIALGMGAKTYKLKFGHRGT
                     NQPVKNLLTQKIEITSQNHGFAVDYESIKHNSDVEITHINCNDTTVEGLRHKKLPLFS
                     VQYHPEANPGPHDSRYLFQQFYELVTH"
     gene            33445..35727
                     /locus_tag="PLJ75_03920"
     CDS             33445..35727
                     /locus_tag="PLJ75_03920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPD04396.1"
                     /translation="MDIWNDIVIDSDREPNLTNNAQIVLEKRYLAKDEEGKVIETPRQ
                     MFERIAKYIASADFLYGRDSKHVSQVATTFYDMMVDLLFIPNSPTLMNAGRPLGQLSA
                     CFVLPVEDSMDAIFDAIKYMALIHKSGGGTGFSFSKLRPKNDVVQTTKGVSSGPVSFM
                     NIFNTATETIKQGGTRRGANMAILHVTHPDILEFIKAKEKNDALTNFNCSVALSDAFM
                     NAVENDDDYELINPRNNRVSGTLKAREVFNLIVRMAWKNGEPGIVFIDRINEKNVLKK
                     VGLIESTNPCGEQPLLPYESCNLGSINVARCVVNNGKSVRVDYELLNDITYKAVHFLD
                     NVIDVNNYPLPIIGEKTKANRKIGLGIMGFADMLIALGIPYDSEAALGVAKNIMSAIQ
                     DASKEASRALAKERGAFPNFPLSEYAHNGDEPLRNATTTTIAPTGTISIIAGTSSGVE
                     PIFALAYVRNVLDNSRLIESNPLFVEYCKERGLYTDELMQKVAQTGSVAHIEELPEDV
                     RALFVTAHDIAPEWHVRIQAAFQEYVDNAVSKTVNFPHSATVGDIETVYMLAYHLGCK
                     GITVYRDGSRETQVLQVEKGKQPVPIMFNIQPRPRKEVTWGKTLKMNTGCGSLYVTIN
                     EDEYGLFEVFATMGKAGGCAASQAEAVSRLISLGLRSGIEPEQIIKQLKGVRCPNQAW
                     VMGGRIYSCADAIAKAIERYIHPDAVQEQNEDIHKNIAETNGKGSDTVMVGVCPECHG
                     PLEFESGCSVCRICGFSRCG"
     gene            35731..36549
                     /locus_tag="PLJ75_03925"
     CDS             35731..36549
                     /locus_tag="PLJ75_03925"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPD04397.1"
                     /translation="MYQVYFTADIDEQLSEKLALYAQSISILYDEKAIIALFDEEDEI
                     PSFAKDYILQINNLEEEVWKYRYLDYLTGVAITPSIYVYPTTKALGGDSSYTYTIALN
                     PKDAFGDGTHPTTRMCIQQLEKIVNTFSLEERRSLTLCDIGTGTGIIAIVAAMLGIGT
                     VEAFDIDTTSVERAKENAAQNGCNRILFFVSDIANVNTYKKYKIIVANLLSDIIEKNV
                     KNIVSLVEKGGTIILSGVGHRWVKGIIQLFQQCGCTPIHDESMSTWHCMVLKAK"
     gene            36552..37559
                     /locus_tag="PLJ75_03930"
     CDS             36552..37559
                     /locus_tag="PLJ75_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD04398.1"
                     /translation="MKIKNIKEVYPGIFRIVEYRSLKVTPPINLYIIAGSDGLLFDGG
                     YGDDNSIQIVGDAIDYVHSLNEKWRITRVLLSHTHPDHFAGLRRLQQQYHLKVMLTEP
                     MTHVIANRKSYIHANKEDGVLKKRYSTIKQRLAALPLEFFYQKMFKKLYGIQYVPNPD
                     IIISPQDTITVDNAKWKIIPGPGHCADHIMLYNKKQGILFGGDNVLRSIYTWLGPPRS
                     DLQAYMQTLHTCLQLPHLQCILGAHGKPVTNPRQRIEQIIEYRKSRTKQIYSIIANAP
                     NGGITFDRLMNMMYPKKNGHRWLGRGWIIVTIEYLVNKNALRFQDKKIIANPVDINSV
                     L"
     gene            complement(37543..38508)
                     /locus_tag="PLJ75_03935"
     CDS             complement(37543..38508)
                     /locus_tag="PLJ75_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD04399.1"
                     /translation="MDSNYRPWILLFAFLMLLPGCTSLGMRSIPVDELKKKYASASSR
                     FIVIEDVTIHYRDEGKKDAPVLLLLHGVCASLHTWDGWVALLKDDYRIIRVDAPGFGL
                     TGPISSGNYTPEYYVELINKMLEQLSIPELSIAGNSLGGYMAWQFALKYPQKVEKLIL
                     IDSVGFPQEMPWLLKFSTNALVRPFARSAMPRYFINKAVHEVYGDQSKVTKEVVDRYF
                     ELSQREGNKSAWIDIFMVMKEYSKREDLSKGLTDIHCPIMVMWGTKDIWIPYTTEFPK
                     WQKALPHATFKVYEGAGHIPMEEIPEVTAHDAFAFLTGKELTARN"
     gene            39498..39785
                     /locus_tag="PLJ75_03940"
     CDS             39498..39785
                     /locus_tag="PLJ75_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HPD04400.1"
                     /translation="MLSIKVIPKSSKNSIECVDGVYRVHVNAPPVDEKANKSLIAFLA
                     KVLDVPKSSVTIAHGQKARNKNVKISGLDAEAVKLRIENFAQRKAQRRYDE"
     gene            39778..40173
                     /locus_tag="PLJ75_03945"
     CDS             39778..40173
                     /locus_tag="PLJ75_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04401.1"
                     /translation="MNSKNTSNGIYCIAIILCLHYCACSSGSTVTVKEGCIDNNVLRV
                     VVYVPYSELPQKKLTSDELLTIALQRSEQRYQQIIVNALQLSSGKRDELSPSYSNRTI
                     ALKQKRVDGYLIMVDYVLYDEIAQLLQCR"
     gene            40106..41011
                     /locus_tag="PLJ75_03950"
     CDS             40106..41011
                     /locus_tag="PLJ75_03950"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HPD04402.1"
                     /translation="MDTLLWLIMFYTMKLRNFYNADKLYCSMALKPLKTEDSTCTFFD
                     DEYNEAMHSLSGAYREALYMHVLPSKVLEKEGTVCVLDVGLGLGYNVLALLYEARKRN
                     ANAFYHIVSFEKNPAIPCTMETIMFHDDRDERYKLIRKAAVEKEVTTAWYNHVVLYGD
                     ARKLIASMPEDYFDAVFHDPFSPAKNPELWTVDFFKKIYHVCRDDGIVTTYSAALHVR
                     AAMHQAGFRVGIGPQVGRKKEGTVATKKNAAVVPLGDDYFTALKTIAKAIPFTDVLLC
                     DDPELIRQRRHEAMHAFKKGHQALE"
     gene            complement(40983..>41272)
                     /locus_tag="PLJ75_03955"
     CDS             complement(40983..>41272)
                     /locus_tag="PLJ75_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04403.1"
                     /translation="DNPQDFLEFDINVESAHLLQDKKVYEAIHLAYFYACALSYRLQE
                     GDLAGDVAFKDGETPLAIVQHNEKSSSASEPGDTSTKKESSSVIQELDDLF"
BASE COUNT        12980 a         7149 c         8878 g        12165 t
ORIGIN      
        1 tgcgggcaat acaaaggctt attggcatgg atatccctct tattatggta tccagcaaga
       61 cgcattctga aataatgaag ctgcacggtg cactgggttt acaccatccc tttgtttttg
      121 aaaacggtgc aggtattggt tttgctgatg gcagctatat attgctgggg aaagatatac
      181 aggaactatc gaaccataaa accataatac gtgccatggt aaagaattgt atatgggttg
      241 acgacattcc ggttcaggaa ctatcgctct ataccgggct atcacatgat gatgttaccc
      301 ttatgctcca gcggaaggca tctttgttgt ttatggcaga acaattgcca acaaccatta
      361 cggacatcaa tagcagtatt gctgagtatg gcattgccat cacaacagga gggaagtttt
      421 ttaccgtgca ggatagccgg gttgataagg gcagggcagt acagtacatt aaaaaccttt
      481 ttcaaaatag atataatgat atgtatacca ttgggatagg tgatggctat aacgatatcg
      541 atatgttcaa tgctgttgat gaggcatatt ttgttggcga tgatacgcta tttatcagca
      601 ttcagggaca gtgcaataag gttacaatga cacagcgaaa aggtcccgat gggttttctg
      661 atatcataaa tatgataacg caacctaatc gctgacctga acatgctgct ctacatagct
      721 atcaaattct tcacgtgtca ttttgtcctt gtacaactgc cacatcactt cttttaattc
      781 gtctaatgtt tcaaacttag cgccgtcaag tttataatgt gccatacgtc atgtccctgc
      841 cagcaaaaat tattctgatt tatcctgatt gatcagaatt agttctttac aatcacgggg
      901 tcactataaa atttagcaca aaatattcca taaaaaaata tttgaaatat tcttgataac
      961 tttttattgt atgatgggat taaagaatga aaagatatat aacaataaat attttactat
     1021 tagtatttct atgtgcgata gttaccggtg gattatgtca aacattatat attgatgaca
     1081 ccttcacgtt ttctgaagcg ggattgcacc ttgactattt aaccggggtt gatccctcca
     1141 caggaataga tgaaattgtt aaacgataca acaatcatga atttaaacca tcaccatcag
     1201 tgcatccctc atttggctat tcaacacagt gtttatggct tagccttaca gtaaccaata
     1261 ccacatcgct gcctgtgcag tggttgctgg agtatgatta tccgcttatc gatgacatta
     1321 ccctgtacat tccccatgac cattctttca ctgcaataca gacaggcgac aggctgccat
     1381 tttcacacag gcaaattccc taccgtacct ttgtattccc gatagtgcag aagccgggta
     1441 caaccaccta ttttatgcgg gtacagtcgc agggctcgct ggtcgtagct ttaaaagcgt
     1501 ggactccaca cgcttttgaa gaaatgagga ataaaacgct accttttatc tggatgcact
     1561 atggtttgat gattgcgctc tttttctata acctgttttt atttataacg gtacgggata
     1621 aaagctttct ataccttgta atatttatta ttggggttac cctttttgca atggcaaata
     1681 atggtattgc tgcacaatac ctatggccgg aaagtccatg gtgggggaat gtgtgtcatc
     1741 ctttcttcat ctttttggca atggttgggg tagttttatt tacacgggag ttcttaaatt
     1801 taccggttgt aatccctgta ctgggcatag ttttacttat aatggcaatt gtaacggcta
     1861 tgggtggtat tatccccttt gtggcgccct actactatgc aacgcagatt tctgttttaa
     1921 tggcagctat tgaagccatt attgccatta taataggtat tgttgcacag attaaaaaag
     1981 taaaggaagc tctgtatttt aatgttgcta catttagctt tgtagcaggt gtcatcttaa
     2041 tagtattgcg ttcatttggc atgctgggcg ataacttctt tacggcatgg ggatatcagt
     2101 taggctcttc ggcaatggtt attttgtttt cattggggat tgcggataag gttaacacgt
     2161 ggcgtaagga taaggagcgt gctttatcgc agcttgcaac ctcagaggca aagtaccgta
     2221 ccctggttga gaatgctcat gatggcatta tgcttgttca gaactggaac atggtatata
     2281 gtaacaatgc acttgcacgg atgcttggat ataccgtaga agaattgtta aataaaaata
     2341 tcaattttat cactgctgat tcatcgctgg ggcaggaggt aataatacaa tatgaaaaca
     2401 ggatgcttgg gaaagcagct aagttgcagt atgaagcgca gttaaagcgt aaaacaggca
     2461 ccgttatgga tgtattgatt tcagcacaga aggttatact ggatggtata tctacaagca
     2521 ttgctatcat taccgatatc tcttccttaa aggaggctca ctacacaata caacagcaat
     2581 acgaagagat acaatcacag tatgaagaac tggaagcaat gaatgaagag atggtacgca
     2641 cgcactatga acttactgaa gccacccata atttagagga ggagaaagag cgtctccatg
     2701 ctaccctgcg atctatatat gatgccgtta ttgcaacaga tatacaggga aaaatcatta
     2761 ttatcaatgc gcgcgctgag caacttttag ggaatataag tactgaagtc atagggaatc
     2821 agtttgttac tgtatttccc ctgcggtaca ctaccatgga agaatttaaa aatcctgtga
     2881 atgagatatt acgctatggg tcgcttcata ctgctggaga accactggtg ttgcagttac
     2941 atacaaaaga aattattgtg gagctatcgg gtgcccccat ctatgtggaa ggaaagatca
     3001 ttgggactgt catagtactg catgatatta cccagcagta ctggatggaa aaagagatgc
     3061 acaaaataag taaattagaa tcactcagta ttttagcagg tggcattgct catgatttta
     3121 ataatctatt gactgctatt attggaaatt tatcaattct tggaatgaag ctgtccgaga
     3181 atacagtata tcaaaaatat attgaaagga ttgaaaatgc cgctcaacgc gctgttgatc
     3241 ttactaagca gcttttaacc ttttcaaaag gtggcgagcc aataaaagcg gttgcttcac
     3301 tggaacagtt gattaccgat acagcacagt ttgttttttc aggctcaaat gtacggtgcc
     3361 atcttgatat tcagcctgaa ctatggaact gcgaaataga tgccgggcag atgggtcagg
     3421 tttttcaaaa catcatgata aatgccatgc aatccatgcc gcagggtggc attgttacaa
     3481 ttaaagcata taattatgtc tacgatggca aagcccagat accactttta ccaggggatt
     3541 atattgccat aaccattaag gatgaaggta taggtatacc agaagaaaat ttgtccaaaa
     3601 tatttgatcc attttttacc acaaaagagc acggcagcgg attgggactt gcaacaagct
     3661 attctataat caagcggcat ggaggttata ttgaggttac ttccgatgta gggaaaggtt
     3721 cggaatttat tatctatttg catgcaaccc atgaaacaaa tgaagaaaaa aaggaaggat
     3781 cggcaatcat taattcatat gaaggtaaaa ttttattgat ggatgatgat tttaccatct
     3841 atgaggtagt ttcacatatg ctggatatgt acggatttac agttgattgg gtagaatctg
     3901 gtgaaaaagc aattgctcag tataaaaaat cgttagatga aaaaaggccc tatgatatca
     3961 tcatcatgga tttaacggtg cccggcgcta tgggaggagc gcaggctaca aaagaaattc
     4021 taaggattaa tcctgatgca aaggttatcg tatcaagcgg ttattccaat gatccaataa
     4081 tgtcccatta caaagaatat ggatttaaag gcgttattgc caaaccatac cgcatagaag
     4141 agatcatgaa tgtaataaat gatctcatga agcagtaaca attgtatgca atccatccga
     4201 taactatagt gtacaacggc agttatcatt tttactggcg atagttttag aggataaagg
     4261 tatagtatga tgaactatcg cacacaggta aataaaacat tgtgggatag gaacacagta
     4321 tgaaaaaaca atgcattgta tttattggta ttgcagcctt actgacaata aatgcctcgg
     4381 tggtaaaaca aaaacaatcg gattctgcaa aggtaaagga gcagtacagc aatgacataa
     4441 agaggaaaga agatatccag aaaaagatat atgatttaat gaaagagatc aatgcacatg
     4501 caggcggtga tgcattgata gtgccggttg atcctgagaa ggcagacaat gttgttgacc
     4561 atgatgacta tcaaaaaaaa gaagcaggtg aaaaaaaggc tgatgacaaa aaaactgatg
     4621 atacagcatt gatagcacca acaaaaggca aaaccacaag caaatatgga taccggcaga
     4681 atcctgttac caaaaaatat gcattccaca aggggattga tattgctgca ccaaagggta
     4741 caaaggttgt agcaagtgct ggtggcgttg ttgcaacatc aaactattta aacaatgggt
     4801 atggcaattg tgttattatt gagcatgcaa acaacgtaaa aacactctat gcacatcttg
     4861 cgcagagaac agtagctgct ggcgatacag tcattcaggg acaaaagatt gggacggtag
     4921 gtgctacagg aagggctacc ggcccgcatc tgcactatga ggtatataaa ggcggaaaat
     4981 caataaatcc tgaggatctg gtacactact aagaaggtgt gttgattata tgaataaaaa
     5041 agcgtgtata ctgggtgcca ccgggttggt aggtggggaa ttactttctt tactactcca
     5101 tgatgattat tatgcacata tcactatcat cgtacgaaaa aagattgcaa taccttcaca
     5161 aaaggttgct gtcattgtta ctgattttgc caatctggaa gatgcgtcag aagcatttca
     5221 agttgatgat gtattttgct gtcttggtac caccatgaaa aaagcaggct caaaggatgc
     5281 cttcaaacag gtagattatg agtatgtgct gaaagctgca gcaattgccc ataaacaggg
     5341 tgcacaacaa tttttagtta taagcgctgt tggtgccaac ccccatgctt atgcatttta
     5401 caacaggata aaagggttga tgaagaagga tgtgtcatca atccccctgc agacaattca
     5461 cattttcagg ccttcgcttt tagtgggtaa aaggcaggag aagcgcctgc ttgaggatat
     5521 agcccgattg tttgcaaagc ccatgcagtt tttgctaaaa ggaagctttg caaagtatgc
     5581 ccctgtcgaa gcccgtactg ttgcagaagc aatggctggt gcagcaaagt tgaatacgaa
     5641 aggagttcat atccacgaag cattataggc gatagttatt gtgcaggtgc ttttttgttg
     5701 cataaaaata ttgttactgt agtgatgaat gatggcagtg ttgccattca tgagtgacag
     5761 agatatagcc gcatgagatg gagaaagcgg ttttttattt ttacagtgca ttaatgtatt
     5821 gacgaacata caggcataat tgttatccct ccacattgaa ctgttataga gtaactatcg
     5881 tacacaaata attattaatt attggaggat tgtatgacgg gtaatgacat taaaacagta
     5941 ttgatcattg ggtcgggtac catgggacag cagattggtt ttgtgtgtgc cctccatgga
     6001 tgtcatgtgc acatgtacga tgtccacgaa gaagcattga agagtgcaca gaataggctg
     6061 acacagttag cacagtggtt tgaaaagcat actgttaaaa atactactaa tgctctgtcc
     6121 cttatacgct attatactga tccacaacag gctgctattg atgttgattt tgtcagcgaa
     6181 tcagtgcccg aaaacccaga attaaaagct aaagtttttt caacatttca tgcactgtgc
     6241 cgtgagcaaa caatatttac caccaatacc tcatcgcttg ttccatcaca gattgctgcc
     6301 gctacaggaa ggcccgacag atttttagca tttcacttcc atgatatcag gaccacaaag
     6361 atagttgatg ttatgccgca tgcgggaacc tcgcaagagg taatcaatgc aactgttgaa
     6421 tttgcaaaaa aaataggtca gataccaatc gttcttaaaa aagaaaacaa tggctatgtt
     6481 tttaatacca tgctcatgac attacttgcc tcggcattga ccctggcttc taatggtgtg
     6541 gctgatatcc atgatattga taaaagcttt atgggcgtta tgggcacacc aattggacca
     6601 tttggtatta tggatagcat cggattggat acggtacatg aaattacaaa atactgggca
     6661 gatttgcttg atgacaaaca gggcaaaaaa aatgctgcat tgttacagca gtatgttgaa
     6721 aaaggcttat taggcactaa aaccaacaaa ggtttttaca cctatccgca tccagaattt
     6781 tccaaaccgg attttttgca gtaatggctt gtaatgtttg attttgttga gccctctata
     6841 gctgtgccca gcaagagtac acggcaattt ttggcgatag taactcttga atgaaattag
     6901 cagtgcatag tagttttatt gtgatgaaac aaaaaatagt attgtttatt ccatgatttt
     6961 gtgatgctat ggcaatggtg atagaatgtc actgataggt atggtatgcg atttgaaata
     7021 ctacaacacg atagcaattg caatgcacgt ctggggcgga tacacactcc tcatgggatg
     7081 gtgcatacac cggtattcat gcctgttgcc acgcgcggag caattcgtgc attgacattc
     7141 cgtgacattg aagaattagg ctttgagata gttctttcaa atacctacca tatgtacata
     7201 cgccccggca ccaatctttt acaaaaagcg ggcgggttgc ataatttcat gaactatcac
     7261 aaacctctat taactgactc agggggattc caggtttttt cactggctga cttacggaag
     7321 gtgcaccagg atggcgttga atttcaatca catattgatg gctcaaagca tttttttact
     7381 cctgagaagg tgctggatat tcaaaggatt ataggttcag atatcatgat ggtgctggat
     7441 gaatgtgttg cctatcctgc tgactatcaa acagctaaaa cagcagtaga cagaacagtg
     7501 cagtgggctg gacggtcata cgaatactgg cataacaatt ttgatattga agcacaggct
     7561 ctttttgcca tagtacaggg tggggtgtat cctgatttgc ggttacagtg ctcaactgaa
     7621 ttgcaacagt ataacttttc aggatttgct gttggaggac tttcagtagg cgaaccaaaa
     7681 tctcaatata gtgacattac tgctgtaaca gtgccggcgc tgccatggga taagccgcgc
     7741 tatatgatgg gagttggcag cccactcgaa attcttgaag caatcaggta tggtgttgat
     7801 atgtttgatt gtgttatgcc aactcgaata gcacgcaatg gtacgctttt tactactaac
     7861 ggcaggataa acataaagtc aggtatctat gctgaagatt ttaatccgtt agatgaacat
     7921 tgtcattgtt atgtgtgtaa aacatttaca cgtgcatatt tgcgccatat ttataaaatg
     7981 aatgaaatag cttctctgat ttataatacc tatcataacc tttattttat gaaatatttt
     8041 atggatacca tacagaaaag tatagcaaac aatcaattta tggatgaata taaccgatgg
     8101 aagaaaatat ataacaatag tgaaataaat actatgtaaa tagtaaaaca acaggataat
     8161 aacctgaatc aaaagtaaat ttttctggaa ttattattgt gtataaataa aaaaaattat
     8221 ttacaaaaca aaaaaaataa aatatcttga cattttgctt ttgttattat agtatttagt
     8281 ttcaatacca tgcaagcatt acttttggga gtagaatgtc tatggatatt acgaaaagaa
     8341 cgagaggtga cgttgttata cttgatattt ctggtgagat tgatctttac aacgccccgg
     8401 aaattaaaga tataataaat gggctcattg aagaaaaaaa atttaatgta atcattaacc
     8461 ttgaaaaagt aacatatatt gattcgtcgg gtattggtgc tttaatttca agcttatcaa
     8521 atctaaaaaa atatcagggt ggtttaaaaa taattaatgt ttttgcttcg gttcggaaag
     8581 tttttgaact gactaaactt acgtcgtttt ttgaaatata tgattctgaa gaagtagcta
     8641 ttgaatcatt taataaacgg taatgccttg caaagtaaat ggtcgtgtat tcaaaaaaat
     8701 tacccatggc cggcatgaca atgtgttggc atgtcattct gaacggagtg aagaatctcg
     8761 tcgttgtata aaagaataga ttcttcggga ctgcgtccct cagagtgaca atgctgggaa
     8821 ggtcatcctg aaccatattt gcatcacttt gtattcatta attgataagc atttgattaa
     8881 taaagagtat tatacattag aaaaatgaaa gaggtgtact atgagatcac aatatcatat
     8941 cagtgttatt gcgctattgt cagctcttgt gctgatgact gcctgctttg aaaagttgcc
     9001 agttaaggag ctatcgcttg caaaaaatgc tatcagtgat gctgaagcag tgaaagcccc
     9061 ctactatgcc aaagatgaat acaatcaggc aaaagacttg cttgtagcag cccacgataa
     9121 tgtaaaaaat gaagagtatg ataaggcaca gcaaaatgca attgaagcac aaaagaaagc
     9181 cagagaagca tatgataaag ctattccatt gcttgccaaa gatactcttg atatagctac
     9241 caagagcgtt gaagctgctg ataatgtgta tgcaggtgtt cttgcaaagg atgattttgc
     9301 caaagccaat gaaatgttgc aggaagcaaa caaaaactat gaaaataaaa attatttagc
     9361 tacctacaaa gctgctctgg atgcagatat gtatgccaag aatgcacgca atgttgcatt
     9421 gagccagaaa gaaactttag ctgattcaat caaggaagtc aacattatta tagcacaggc
     9481 tgagagctat aatgctaacc agtatgctcc tgaaaaatta aaacttgcaa aggagcagtc
     9541 ccaggtagcg cgtagtgctt atgatgcaga agagctcaag aaaggttttt cagccattga
     9601 agttgccaag ctcaatgccg atgaagcata ttttgcatca ttaaaggcta ctgcagctaa
     9661 gaaaattgat gaagcacagg gagcaatagt agcagcaaaa gctaccgaac aggcaaaaca
     9721 atctccagac gatattaaag cagcagaaga atcgctttct aatgcaaaaa ctttatttaa
     9781 tgatgcaaaa tataaagaat caataattgc ctccggtgag gcaattacaa ttgcaaattc
     9841 agtatcagca aaaaaagtag ccgtggctcc ggtcattgaa agcgaggtaa caccggaagg
     9901 taaaaaagaa gtgcaatcag aaactcagaa ggaagcattg cagaaaaaag tagaagaaga
     9961 aaccgaatac accatgtata aggtaaaata taatcccgaa aaaagagatt gtttatggcg
    10021 gatagctgta aaattttaca atgatggatt taaatggaaa ttaatatatg aagcaaataa
    10081 agatttagta aaagatccca atcttattaa gccaggctgg gttttgaaga tacccaaaac
    10141 tgccacaaag cttgcacagc cgaaaaagga aggcgttaac gagcagacag cagttaagga
    10201 agatgaggca ccaactactg aattgaagat gaaaactcag gaataaatta aagaaagggg
    10261 tgtttgcgac accccttttt aatgtcattt ttgttttgat tcatcccaca atgcatccag
    10321 ttcacttaac ggtgtttgag caatatcttt gttcatttca tttaatcgtt gttcaatata
    10381 gtggaatcgt tttgtaaact ttgcaatagt gtgttgtaaa gcttcttcag ggtttatttc
    10441 cagatggcgt gcaatgttaa caatggtgaa aaaaatatcg ccaatttcat cttttaatgc
    10501 ttctttattt tctttatttt tttgcaattc tacggcaaac tcattcactt cttcattcag
    10561 ttttgctata atatcatgta acttttccca atcaaagcca acgcgcgcta ccttctgctg
    10621 aacgcgatat gcttttagta aagctggtag atggtaggga acgccatcga gcagtgactg
    10681 accgctattt ttttccagct tttttatctt ttcccagcgg tttataactt catatttatc
    10741 gtttgcagtt tcattcccaa atacatgagg atgacggcgg attaattttt gaatgatact
    10801 attcgcaata tcatcaatat caaataaatc atgttcgctg gcaatctgtg catgagcata
    10861 aacctggtat aacaaatccc ccagttcctc tttaagatta tcaatatctc catgttcaat
    10921 ggcctgatat acctcatagg cttcttcaat aagataagat ttgagtgtgt cagaggtctg
    10981 tgccttatcc catgcgcatc catgttcact gcgtaatatg cctgcaattt cttttaaatg
    11041 atataatggt gattgtgaca tgattgtaat ccttcataat aattttttta acttttctat
    11101 accgatactg atactgatca tcatacattg tgacaatagt ctacttataa aaaatgtatt
    11161 gaccactatt ttagacatag tataccacac caaaaaaaga aaaatttttt taggttgaca
    11221 tcattttata attgtgaaca tacgcactat gtggcataca tgattatgta aatatagatt
    11281 acaattctat caatgaggta cattcatggc attgcataaa tttttaaagg atgctgaccc
    11341 tgaagtattt tctatcttgc aaaaggaaaa catgcgtcag gaaaattctt tgatacttat
    11401 agcatcagaa aattatacgt cacaagcagt actggaagca tcatcctgca caatgaccaa
    11461 taaatatgcg gaaggatatc ccggaaagcg ctattacaat ggctgtcagt ttgtcgatga
    11521 gatggaacgt ttagctattg aacgaataaa aaaaattttt aacgcagatg ttgccaatgt
    11581 gcagccacac agtggtacgc aggcaaacat ggctgtgctc atggcactgc ttgagccggg
    11641 caataccata atgggtatga acctatcaca tggcgggcac ctatcgcatg gttcgcctgt
    11701 taatttttcg ggtatatact ttaaagtggt ttcttatggc gttacaaagg atactcaacg
    11761 tattaactat gatgaggcat acagcgttgc aaaagaacat aagccaaaaa taatcattgc
    11821 aggagcatcg gcttatccac gcaccattga ctggaataag ttcagggaaa tagctgatga
    11881 agtaggcgca gtgctgttaa cagatattgc tcatatagca gggttggttg ccacggggca
    11941 tcatccatcg ccagtgcctg ttgcagactt tgttaccatg accacgcata aaaccctgcg
    12001 aggaccgcgc ggtggaatca tcctgacaaa gcaaaagtat gaaggcatga tgaacaaata
    12061 tatcttccct ggcatacagg gaggaccttt aatgaatact atcgccgcaa aagctgttgc
    12121 atttggtgaa gcactgcagc cttcatttaa ggattattca gcaaaggtta ttgcaaatgc
    12181 acaggcgctt gcccggacat tgctatcacg aggtcttcat ctggtatcag gaggtaccga
    12241 taatcatctc atgctggtag acctccacaa caaaaatttg acagggaaag atgtggctaa
    12301 ccggctggag gaagcaggaa taacctgtaa taaaaatggt gtgccgtttg atgataagtc
    12361 ccctatgatt accagtggta ttagattggg gagcccggca ttaacaacac gagggcttaa
    12421 tgaagatgat tttaagcaca ttggaaatct catttgcgat attattgaca atatgaatga
    12481 tgacaaagta ttgcagcgtg tacaagcgca ggtgcaggag ttatgcaaac actatcctac
    12541 tgacttctta cgtctggagc cacgctaaat atagttcttg actataataa gaacactgtg
    12601 tattacacca gtatataaag taatcaggag gaacgttatg aaaagattaa ttggcgttat
    12661 tatgatatgt ttgatagctg ttgtgggatg taaaaaagaa aagaaatata ctgttgcgcg
    12721 ggaaggtctg gtaaatttaa tttcaggcga tgttgttctg gtggtgaatg gtgctgagag
    12781 caaagctcaa ataggtgatg ctattaaaga agggatgatc attaaaactg ttggtccaaa
    12841 gtcaattgcc gagatatatt ttggcgaaaa tgcagtgaag attttaggtg acacggttat
    12901 caagattgaa aaattgatta ccgatattag cgcaaatacc gaagcatcat cgtttgtagt
    12961 tgaaaaaggg cgtatgttct cacgggtaac taaaaaatta aataagggcg atagttacca
    13021 ggtaaaaaca ccaactgcga tagcagctgt gcgcggtaca gattttctgg taacacagga
    13081 agaagataag agtaatgtag catgtctgga tggactactt gaagtattaa accagagctt
    13141 agctacagga caacctatag tacttgagga aaaacaggaa gttgacattg tacctggtca
    13201 ggatatggtg aaaaaacaaa tatccgagga taaactcagg atgttgaaca tactgctgga
    13261 gatacaggca atgcgtgagg atatccgtaa gaagtatgaa cagcaaaaag aagaaatatt
    13321 aaaatatgtg gaagagcaga aggaagccaa taaaacaatg ctggaagagc agcgtgaaaa
    13381 ggataaagcg cttgttgagg aacagaaagc acgtgacaaa gctatgattg aaggcataaa
    13441 aggccagatt aaagacgaat cagggaaggc agcacagcag tcactggatg cagcaaagag
    13501 tcagatgcag gatgcaaagg ctgttgacaa ggatcaggca agctctgaag ctaaagatca
    13561 gatgagtgcc atgaagccaa agattgaaaa gcaaaagatt gatttgaatc agtttaagac
    13621 tcagtaacag aaaaataatt attgtaaaat caattatata atcatcaggg ctgttacaca
    13681 gtatagtaac agcctttcta ttgttaatgg tagtatattt tagtatgttt aataatagta
    13741 ctattttcct gtttatgagg ggtttgtaga aattttaaca ggcaatgttc taattccggg
    13801 attatggcat gaacaattaa tgaagtgctt ttaatagtaa aatgtaatct cataaaatca
    13861 caatgaatta atttcacgcc gggggaattt tttaatattt tgaagccctt aaaattatga
    13921 tgtttttgaa tagctttaaa aagaaaaaaa atactgaaat aattatttaa caacaaattc
    13981 cttgaaataa acttctttaa tcttcccttt tgttaaaagg gaatttatgt gagcctttat
    14041 ttcctctgct aaatttactg tatcctctac ggaatttact tcttcagaag atttgtcttt
    14101 aattagtatc ctgcatatgt gatttattgc atcttttctt tgttttaatt ctgattctaa
    14161 atcttcattt tcttcatatc ctaagctcat ggttatcctt aaaaaatgtg gttttttatc
    14221 gttcgttttt gtatctattg gagcaatatc aaattttgat agggagggtg gtggagcaag
    14281 tattatgccc tgttcttctt caaattgctt ttccagtttt gatttagcca tataataact
    14341 taatttaaat gctattttgt gcagtaaatc ttccagtcca catcccgaaa gtgatactat
    14401 taataatagt gaaataaaat gtattctttt cataattatt tcctcatttt agtataagga
    14461 tatttaatgg attacagatt gaaaaataaa agtcaagtgg ttttcaaaaa aaagatatag
    14521 tgcaggttat cgtttttgat tatattttgg ctaactgcaa aagtataact ttaaatgaaa
    14581 aattgttgta aagaccttac aaggcatatt ttggtttttt gataatattg ttttgaaaag
    14641 ttatttcccc aaatactaaa gttatgtata aatattgtta ttgactaatt tgcttatatg
    14701 ctggaaactt ttgcacagca ttggtactat tttataatac tattgaatga tggcattacg
    14761 ttatgataca attagcgttc ataggtacgg ggacatgtaa tgccacggct aggaagccgc
    14821 aggcgctggc attttccatt gatggggagc tggtgtgtgt tgattttggc ggaggagctt
    14881 accatgccat agcttcatta aataatccgg attttaacta tggcaatata tcaaccgttg
    14941 tattaaccca ttaccatgtg gatcacatat cgggtttgcc tgatctactc tggggtgaga
    15001 tgtgggacta taccggacac cgcacagaac cgttaacgtt tgtaggacca aaagggttga
    15061 agaattttta tgaagacaga ctgctacctt ttatggggga ttatccacta ccgtttaaag
    15121 tagagcttgt tgaattgcat gatggcgata tttataaagg caatggtttt acgctccaat
    15181 caaatactct tgcccatggg gatttttcgt caggctacct ttttgattgt ggatcaaaaa
    15241 aaatagcaat aacaggtgat accggatact gtgacgcatt acaacgatta ctttccgttg
    15301 ctgatatggc agtcattgaa tggggaatag cgtctgatga tacctatcct ctccatgtat
    15361 cgtcaaagga tattatatcc ttagtcaatg cagggatatg gccacagcag gtgtatgctg
    15421 tacatgtata cccattgcca gatcttgatt ttgaaacaca gttacaaaca atgcgctctg
    15481 tggttgcttc acataataaa tccattgaat ttccttccga tggcgatata ataacaatta
    15541 ttccataagc tattgcaata atttttaggt agcacacatg cttaatcatg atgttcgtta
    15601 agctgtatat agtactttat tattacgatt aattgggaat tgcgaaaagc acatttatga
    15661 aagctataaa agtagactat tgtatcattg gtgcaggtgt tgttggattg gcaatagcac
    15721 gagagctatc gctacgcact aaaaaaacaa tagtggtatg cgaaaaggag gcacaggctg
    15781 gttttcatgc atcaggaaga aatagtggtg tgttgcacgc tggtatttac tacacgcagg
    15841 ggagtttaaa agccaatacc tgtattgaag gcaacaggct tatgaaagag ttctgcgctg
    15901 aaaaaggggt agcagttaaa gaatcaggga aggtgatcgt tgcaaaaaat cagcatgagg
    15961 ttcccaccct ttatgagctt gagcagagag caaaaaataa tggcgtgaca gtagagctaa
    16021 ttaatgaaaa aaaattggag cagattgaac cgtgtgccag aactaacacg cttgcgctgt
    16081 tttcgcctca tacagcagtt gttgacccaa aagtggttat gcaggcattg taccatgact
    16141 gcattcaacg ggggataacg gtattgcttt caacgccatt caaacaattt accagcgata
    16201 ccagcattat agcaggtaac catactatta cctatgataa gcttatcaat tgtgcaggag
    16261 cacacagcga taccattgct cacaccaaag cccttgcaaa gcatctggcg cttattccct
    16321 ttaagggcat ttacatcaga ctgaaaccag atagcaagcc tgcaatcaac gggaatatct
    16381 atccagtccc ggatatacgc aatccctttt taggcgtaca ttttaccaga gatccatacg
    16441 gcaatgtgta cataggtcca acggcaatgc ctgtttttgg cagggaacan nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt tgaagatttt atgcgtcccg acagggtcat
    16621 cattggtacg gatgacgaag aggcactggc agtaatgaaa gaattatatg caccgtttac
    16681 ccatagtcat gaacggttta tagccatgga catccgcagt gcagaaatga caaaatatac
    16741 agccaatgcc atgcttgcaa ccaaaataag ttttatgaat gagatagcaa acatttgtga
    16801 aagggttggc gcagatgtaa atatggtacg tattggtatt ggcagcgatc acaggattgg
    16861 ctatagcttc atatatccag gtgttggtta tggcggctca tgttttccaa aagatgttca
    16921 ggcactaata aaaacagcaa aagattacgg gtatgaacca aaagttatta ctgcggttga
    16981 agaagttaac aataagcaga agctggtact ggtggataag gttgtacagc gttttggtga
    17041 ggatttaacc gggaaaattt ttgcactatg ggggctggcg tttaaacctg aaaccgatga
    17101 tatgcgtgaa gcagcaagca tcaccattgt caaagagctt gtaaaaagaa atgcatatgt
    17161 caaagcgtat gatcccaaag caaccgaaca ggcaaagcaa tgttatttgc atgatataaa
    17221 aaatatagaa tacgcaacaa gcaagtacga tgctctgaat gatgctgaag cattattgct
    17281 ggtaaccgaa tggaaagatt tcaggagccc tgactttgat gaaataaaaa aacgattaaa
    17341 aaatcctgta atatttgatg gcagaaatca atataacata aaaaaattac aaaaagatgg
    17401 ctttgaggta tatcagattg gtgtaggtaa tgtcagataa aacaaaattt tttataaccg
    17461 gcagttcagg atttattggc acatacttta tacaattttt agataatagc aatatcaacg
    17521 ctgaagtact tggtgttgat attgtgccct caccattaac gggtacaaaa gctatagttt
    17581 tccatgaaga aatgtgtact atgaacgaca gcaatgccgt gcaattgttg ttacaaaaac
    17641 ataaacctga ttatatcatc cattttgctt cacagagcag tgtggctaaa agctgggatg
    17701 atattcccgc aacattgcat aacaatgtga attcattcat actattagct gagggtgtac
    17761 ggaaatattg ctctgacaca aggatgcttg tcattggttc atccgaagaa tatggcgagg
    17821 gtgcacctga caatttacct atagaagaaa cctgcccatt acatccacaa aacccgtatg
    17881 caattgcgcg ggttgcacag gagatgcttg caattatgta tgtgaaaagt tttagacttg
    17941 atattgtcat gacacgttcc tttaatcata tcggtccggg tcagagcgaa cattttgcca
    18001 taccatcatt tgtaaaacag ataataacag caaaaaataa taatacatca tgcatactaa
    18061 aaacaggcaa catagatgtt gtccgtgatt ttgttgatgt tcgggatgcc gtaaaggcat
    18121 actatctatt gctgaagcag ggtaaaaccg gtgatgttta taatatttgc agcggggttg
    18181 gttattcatt gcgtgctatc atcaccatgt tatctgaaat aaccagtgtg caggtagtca
    18241 tagagcatga tcaaagtaaa atgcgacctg cagaggtgca aaccattatt ggctcatatc
    18301 aaaaaataaa tacattaaca ggatggagcc caactataaa catccgccaa tcactggaag
    18361 atattgttaa gggtcagagc tctgccttac attaaaaaaa gtatcgtgcg cctaaaccac
    18421 cacggatgcc aaaatcaaca tcaggtgcaa tcctgagcgc tggcactaac tcaaaaaata
    18481 tccgtattgg tacttcacta atggtattag cagcaaaacc agagtgtgat gcaaaaatca
    18541 cagttgctat tatgaaaaaa aatactaatc gcttcatagt agtctcccct tgctatagta
    18601 aaccattgat aaaccgtttg tggcattgca ttgtttactg tgaatacata attttatcta
    18661 taaaattata aaaaatactc tctactggca actgctaata cttacaatta ttatttatta
    18721 tattatttat tactgtacac tgttcatgat gggcatcttc accaatcaaa tttttcattg
    18781 atgaataata atccactatt gtgcactatt tgtgattatt gggggatagc gtaacaacaa
    18841 ttattaaaaa actgctactt cacctgttat actgttactg agtaactatc gtgacaacaa
    18901 aaagaaaatt ttttatctac ttttgtttgc ggtactatac tatatagggt aattattatt
    18961 tcttaattaa taggtgctga tatgcgattg cgacaaacat tattgaagat tttaatcaat
    19021 ttttatcctc catttttagg agcaggaata cgcattagat ttgataaaaa aaataaaaaa
    19081 gtctatgtat ccatgaagtt gacctggtac aaccgtaatt atgtcggtac gcattttggc
    19141 ggctccatct atgccatgtg cgatcccttt tacatgtttt tgctcatgga aattctggga
    19201 agcaactata ttgtgtggga taaaaaagca acaatagatt ttattaagcc cgggaaaagt
    19261 acactgcatg ctgaattttt tatagatgaa caaacagtgg aagctattaa aacagaggtg
    19321 gaacaaaaag ggaaagcatt gcctcaattc actgttgcag taaaagatga cgatggcgag
    19381 attattgccc gggtggataa gtatatttat gtgaagaaga aaaaaacatg atttaccctg
    19441 caactgacat taccatacaa caggcagccg aagcaataaa acagggtgag gtggtagcct
    19501 tccccactga aacggtatat ggattgggtg caaatgcatt tgataggctg gctgttgcaa
    19561 gtatatttgc tataaaagaa cgcccccatt ttgatccctt gattgtgcat gtcaattctc
    19621 tgtccatggt ttccatggtg gcggctgcca taccatgtgc agcaatgaag tgtatggaaa
    19681 agttctggcc aggaccattg acaatagttt taccaaagaa ggatgatatc cctgatatag
    19741 taacagcggg gcttgcaacg gttgccatac gcatgccatt gcatccggtt gcgctcaggc
    19801 ttatagaatt agcaggtgtg cctgtagcag cacccagtgc aaatcctttt ggctatttaa
    19861 gcccaactac cgcacaacat gttgctatgc agttgggcaa taaagttgct atcattcttg
    19921 atggtgggaa atgtgctgtt ggggttgaat ccaccattat aaaaattgga aatgatgaaa
    19981 ttaatgtact tcgctatggc ggtatacccg ttgaggaaat tgaaagaaca accggacttg
    20041 tggtcacttc tcataccacc acaattgaag ctccgggtca gttgccttac cattacgcac
    20101 cgcaaacaaa gattgtactg gttagcgata tcatgtctgt tcctgacgat gcaaccagtg
    20161 ggctattgct ttttaaaaaa agagatatcc cctataccag tattcacatc atgtacttat
    20221 cggatgaagg tgatatgcgg caggctgccg gcaatctctt ttcatgtttg cacgcgcttg
    20281 atgctatgaa gcttaaggtt atctatgctg agcaggtgga ggaggttggt ctgggcaggg
    20341 caatcatgga tagattaaaa aaagcacagg cgcgctttat gtagctatgg ataaaaagta
    20401 aattgatatg gattatatga aaagttacca ttataatgct taataacctg acaagaataa
    20461 tagtatacaa aatgccgggg cgatagcaat ggcttccttt gttgctcttt tcttactata
    20521 agatagataa aagtaatagt gcgctacttc cgaaacgtgc acacaaccag gattatagca
    20581 acaatattat gattgacatt ttgataatgt gcacatagta tcttgtgctt tgaaaataat
    20641 taatagcaga atcttatccc gggtattttg ataaaaagga gagcatgatg atacgctttg
    20701 attataacta ttttatggat gaacatattg gcacacagca tggattgtca caaaatgata
    20761 tagatggaat aagacctgaa tttaatgaag tccttaaacg catggatgat gaaaaggcag
    20821 aaggattgct tggtttttta gaaataccgt ttaacacttc cttagtcaaa gacatacaaa
    20881 gttttagaca acatactgat tggtgtgata ccattgcgct tattggtatt ggcggctcgg
    20941 cgttgggtcc ccaggcactt aagaatgcac tggctgatat ctattggaat gagttgcctg
    21001 atgaaaaacg gggtaaacga tgcaaagttt atttctttga taatgttgat ccaattgaga
    21061 tgaactcact gttagatatt atcaatatac caacaacgtt gtttcttgtt atatcaaaat
    21121 caggaggaac taccgaaaca aatgcaaatt ttgctatttt gttagataca attaaaaaga
    21181 atacacgcga ttataaaaaa catattgtta ccattactga tcctgcaaaa gggataatgc
    21241 ggcagattac acgcgaggaa ggttttacat catttccaat ccctgcaaac gttggcggac
    21301 ggttttccgt attaacagct gttgggcttg ttatggcaga atttattggt attgatataa
    21361 atggtctctt gcatggagca gcagaagtta tcgatagata ttatggcaag gaggtatgga
    21421 gcaatgcacc gctgcttaat gctgcatttc attattgctt tgatgttaaa aagaataaac
    21481 gcatcaatgt gcttatgcca tatacgcgaa aactatttct ctttgctgat tggtttagac
    21541 agctttgggc agaatcgctg ggtaaacgct ttgacatata tggcaatgag gttatggtgg
    21601 ggcttactcc tgttgcagcg ctgggtactg ttgatcagca ttctcagctt cagctttatc
    21661 tggaaggacc aaatgataag gtaattacat tccttaaatt gcaggatttt ggtaccgata
    21721 tcaccatccc tgaattttat aaagggaaag ctgagctgga ttatctttct ggaaaaagtt
    21781 tgcgcaagct caatggtttt gaggagcagg ctacagagct tgtattaaaa gaagcatccc
    21841 gaccaaatgc ctcgataatt ttaccggagt tagatgaaag atcagcagga cagcttatta
    21901 tgtttttaga aatgcaaaca gcctacgccg gatatctata taacattaat ccatatgatc
    21961 agccagcggt tgaacagggt aagcgtttta cctttgggct gctccaccgg cccgggtatg
    22021 atgagtatga gcaaaagttt aaaagcgggt atcaaaaaaa agaaaactat atactatgaa
    22081 gataatgacg ccccttttga ttgtctttga aggaattgat ggtgcaggaa aatcaacaca
    22141 ggcgacatta ctgtatgcct tttgcaggga atgtgccaaa acagccttat tacaggaacc
    22201 aacggatagc aggtggggaa aaagtatccg aaaaatattg cagggtgagc tacaggcaac
    22261 aacgcagcag atgcttgatc tttttctact tgatagagcg tatgatgtgg agcacaacat
    22321 caagcctttg ttgcataaag gatatgtggt gatagttgat agatattttt attcaacagc
    22381 agcgtatcag gcaggtgatg gtaacagccc tcatgaaatt gtccgcatga attttgaaaa
    22441 aggttttccg gtccccgaca gagtatattt tattgatatt gaaccggaga ttgctcttac
    22501 aaggattgct atgcgatcag ggaataatcg ggacattttt gaaaaattat cagagctaac
    22561 aagaatccgc agtaactatc tgtcaatagc cgatgacagg ttttgtatta tcaacggcaa
    22621 taacagtaaa gaagcaattt ttgatgcaat acaaaaagat tttatacaac attttagtga
    22681 tgtaacgtaa caataccatg aaattattac gtcagctgat aattcaatgt tttaatgttt
    22741 gttttattgc cattctttta ttaatgggat ggatgcttta taatgtccat attgctggtg
    22801 atctgcttat catctttctt attattgtta cagttactgt tttcccgctg tatacctatt
    22861 ggatagaaca tatccataaa aaaattttgc ctttcaggta tgaagatctt tatttacaaa
    22921 ccgttgatat gtttttatcg ttacaatcgt atgatgatat tataaaagtt atatttgaaa
    22981 gggtgttgcg ctttataggg gtgcgctcag gattattaat attttattca catgccaccg
    23041 acgactacac catatactat caaaaacagc aaaaacagaa gataatccgg aaagcccgca
    23101 ttgataaaaa taatattata ttgaagatca tccagtctgg tgatgatata ctgataaaga
    23161 gtaagcttga tccctcgttg cattttcaac gttctattat ctttgaaatg gaaaagcttg
    23221 gcagtgagat catcattccg atatattatc atgatatatt tttaggactt atggttattg
    23281 gtgaaaagaa aaccaggatt tccagaaagg atatattgat gttgaaggca cttgcatcaa
    23341 agattgcgac agttactgtt aatagttttt ttatgcatga actgataaaa aataaagaga
    23401 tagaaaaaga gtatgaacta tcattcaaag tattgaaaaa gtttttgccg cctgacgaag
    23461 gtttcataca caatactcat tacagggtaa tacgccatcc cggaaatgag gcgcattatt
    23521 tctttagcat ctacaattgt agcgatagta tatcgtatgt catcatctgc tcattaagag
    23581 caaatatacc gttgctatcg ctgcttatac ctgccattag tgttttattc cagagttacg
    23641 cacgtttagg ttattatccc cttactatta tagaaaagat caatgccgta ataaagaaaa
    23701 aggaacttat tgaaaaggat ctttctttgc tggtactaaa gaaatgcaac aataattatt
    23761 tcattggtaa atcacgcact tcagatataa aactttttgt ttataatgga tctaccgtta
    23821 aacctgttat gagtaatgat gacagtgcta tgataagaaa tcctgtacgt ttgattatag
    23881 ccagacatga catggcagca atcaaaaaaa atccggaatt gctggataat acagtatcgt
    23941 taagcaacac cataggtgaa aatgaattta ttatgatact tacgagagca taatgaagaa
    24001 acattataga atatatgctt gcattgtttt ttatattgtt ttttttatac tgatagttcc
    24061 acaaagctat cacaatgcat tgcagtatac ctttgatcgg ctggtaattg ggaaaacgtt
    24121 ctattatcca atttcatcgt ttcctaaagc tatgtttcaa tatcatatca tttcgtgcag
    24181 gcaattggcc actaccagta atgatgacgc tggccttgag tgtaccatgg cagggaataa
    24241 tgagatgctg cagcagcaat ttactaataa taacaggact gcagtatgga tagtattgat
    24301 tgttagtatt tcatcgattg tgatagtgct atggggaata ggggtttacc tggcacggca
    24361 tgatatattt tcacagttat ttatgattgt agccgtgtct attgctttat gtttattaat
    24421 gtatatagat gtttttttta cagggaagtt gttcatcatg ttcccggtgg tcaatattct
    24481 ggtgggatgg tcgttgctgc atgtaacata tactatcaat gattatagta agcatggtaa
    24541 aaaagtgtgg tataccgctg ctgcctgcat agctctagtg ctaagcatta tatcattagt
    24601 tagcgatatt gcttttgaaa caatatacat gcttactatc atcatgactc tactgttatt
    24661 tcttgcttcg attatggttt tgacggttac cattaaccat aagctatcat ttgtattacg
    24721 acgaaatctt gtcattgctg ctgctatttt tcttggaggt gtagtgccct atgcaatttt
    24781 attaacagga actatcgccg atatgtatcc tggcatatta tttgctcttt tttttgcaat
    24841 tgccacccct gtcagtgctg gcaatggact tcttgcaagc tcaagtcttg cctccatggc
    24901 aaatctgcaa aaaaacttta tagcaatggt aatggacatt attattgctg ttgtgctggc
    24961 attcagttta ttttatatat ataattttta ttccatttta tccggcatgt attatggtat
    25021 cattattttt gttattgcca tgattatttt gttgcacctg aggatgctta ttgtacggac
    25081 ttttgacgat atcatatttt taaacaatga tgaatatgca ctatccttac aaagaattgc
    25141 cgagattatt acatcgccaa gaagtttgaa ggataaatta gattcaatac agtcagagtt
    25201 gcatacctta atcagggttg aagggataca gtatgccatc attgatgatt atgagtatgg
    25261 tgatagtaga attataaatg taaaatctga ctctgatata gttacctttt ttaaaaaaag
    25321 aaaagaaacc atcacaatga caagttcttt taccaataat gaatatgaaa gggcattgca
    25381 aaggtattgt aaacatgatc acatatcatc atgtattcca gtttatattg atgggatttt
    25441 acagggggtt cttttaatca aaggaaaaga aggtgattat tttacaaacc ctgagctaag
    25501 ttttttagca atgttgtctt tgcaggtaca ccagttgata gttaattcta aagctttagc
    25561 cgaatatatt gcatcacggg actatgaaac ggaaatggac cttgcatcat atatacagtt
    25621 gaggctcttc cccaaaaaat cacctgttga ttgtggcatt cagtttgcaa tatatgcacg
    25681 gccatacctt aaggtaaccg gtgattatta tgatattgtt actattgaca aaaataaggt
    25741 ggcggtggtc attgctgata tttcagggca tggtttgtca gcagcaatga tattatcggc
    25801 aacaagcgcc atcattaatg gtatgttaaa agaaaaaaag aacattagca atgttgttgc
    25861 agaattaaat catttcctta ccatgcgcta tcaggggttt gaactcataa cattatttat
    25921 tggacttttt aataagaaga cacgatcaat ggaatatatt aatgcaggtc atatagctcc
    25981 ggtggtacta tcacacgata agaaatttta tagattggaa gggaggtcaa agattttagg
    26041 ggttgatcct gcggcacaat ttcatgcatc acggtactct ttcaaagctg gtgacgaaat
    26101 gatactgtat accgatgggt tgacagattt atacgacgcc agaaatgatg tatcttttgg
    26161 ggatgaagga ttggaagaag ttttgcaaca aacaattatg aaacctattg aggagaagat
    26221 ccatgcaatt actgaagcaa ttgaggcttt tggacaggaa catatcaaag atgatattac
    26281 catcgttggc atgtatattg attagcatat tctggagcaa tttggcttat gcagaaatga
    26341 aggtgcagcc atccgatggt gtattccctg gtgttaccgc atcggtacct tcgttgccgg
    26401 catatagcta tagcaatcca gtgtatgctg atattaatga aggattttct ttgcagtatc
    26461 gatatgcgta tatagacccg gtaggtgttg gaactcacta tgttgcaact acattttacg
    26521 gctttaccgt tgggtataat acactggaga tagaaattga tgataaagaa attaccagga
    26581 ctgatggcaa ttcatggtat tttgcaaaag gcattatgct tacagactgg tttggttttg
    26641 gaatattata taatagatat acttttgatg gtgaaacggt caaagcgtgg ggatatagct
    26701 tcctgctgca accttcaaag tatctatcat ttgggtgtgt tatagatcat gcaaatgagc
    26761 catcacctgg tttgctttcc ttacggcagc gtgaatggta ttcaatgagc cttcgtcctt
    26821 ttgggaatat gttttcgctt tcatgggata tcaaacgata tgaaggtgag gaatggggcg
    26881 atgtgttgca tatatttacc ctctcaggga tgatgtggca tgatatcctg tatgctgtac
    26941 agtatgattc agataaaaat atttcttttt cactggctgt cccctttgac atttcaacca
    27001 gaacaggcgc gtcatctatc tatgattttg ggtatactaa ttttaacaaa caggagtcac
    27061 atgcgtatac gtttggcatt acccttactg atgaacgatt tagaaatgca ataggtccaa
    27121 aacaaaggtt tttgtcgata gttttacagg agcctgttaa agagataaaa gaaagaggga
    27181 tatttgagcg ctcacgagca tccttctttg aaattgtccg ttcattgcgc gaggcaacgt
    27241 atgatgaaag tataaaaggt gtagtactac agatagatgc gccggtgcta acgtttgcac
    27301 aggcacagga aattcgtgaa gagataaaac ggtgtaaagg aaatgggaaa cccgtatatt
    27361 gtattgtaac gtcagtgggt aatttagaat attacgtagc atcgtctgca accaaaattt
    27421 actatactcc caaccagcca tttggtatta ccggactaaa ggctgaggtg tattttttta
    27481 aaggtcttct ggataaggtt ggtatcaggt ttgaagaggt caaaaaaggt cagtataaat
    27541 catttggcga gtcatttacc cgccagcaca tgtcagatgc agcacgtgaa aatcttatag
    27601 aagtattgaa agaccttaac cagcagttta taagcgatat tgccagtgac cgaaagctga
    27661 ccgtagaaga tgttgaaaag tgcatggcag gtggcatttt gacaccgcag caggctcaag
    27721 cacaggggtt tgttgatgta gttgcccgtc caatggaagt gttgcagagc cttggcgtgg
    27781 agcgatatgg cttatcatac atggtgcagt gtgacgacta tgtagaagaa aagatgtatg
    27841 catatgattg gggtccaaaa cctcagattg cagttatcta tgttgaagga aatattatac
    27901 gtggaactgc taaggataat aattttctat cgccagctat gattggtgat gtaaactata
    27961 aaaaattttt agagaaggtt tttgccgaca gctcagttaa ggcggttgta attcgtataa
    28021 actcgggtgg tggttcggcc attgcatctg atttgatgtg gcattcgctt gtagcattga
    28081 aaaaacgcta taataaaccg gtgatcatct cgtttggcaa tatggcagcc tcaggtgggt
    28141 actacattgc ctgtacaggc gataccatat ttaccagtaa atcaactatc accggctcaa
    28201 ttggtgtact attcggcaag ctgaaccttg aagagttata tgcaaaatta ggcataagca
    28261 aagagacaat caaaatgagt gagtttgctg acatcttttc tgaatccagg gcaatgaccg
    28321 agaaggaaaa gcagcttata caaaagaatg ttgattttac ctataatgaa tttatccaga
    28381 aggtggagat gggcaggaat ataccagccg acgctattcc acaggttgca gaagggaaaa
    28441 tttttactgg ttcgcaagct ctgaacaatg gacttgctga cacacagggc gggcttatcg
    28501 ttgcgttgtc gtatgcagca agtttagcaa atataaaagg tgagtttaag gttatacagt
    28561 taccggaaaa ggatattgat ttacgaaaaa tcttgaaggg aacaagtttt cagacagctc
    28621 tttctgatat gatgcagtat atgtacaata tgctgatttt tgaatctctc aataaagaag
    28681 aggtgttgtt actgtatcct tatgagatag ttattaagta ggggcagggt gttttcccat
    28741 cttatccata ttctcaataa aaatctttgc tggcagggca tggatgaaac gagttagcat
    28801 gaagaaatta cagttattcc tgtgtatggt gtttattctt gcttcaaccg tgctgtatgc
    28861 aaaaccaaag gcaggatttg ggcttattcg tggtttttcc tcagaggaga tgtttctggc
    28921 aacccttctg cggcaacata ttatcaacat agcagcttca ttgcagattt ttgatagtgt
    28981 taatcctgaa aatttagcac ggcaacagca gcgctatgca tgttatgatg agcagtgtat
    29041 gctacttttt accagagaga tgggactgtc gttatttgtc attggtacag taagccagac
    29101 agcaagcggc taccgggtgc atatcgcggc atatgggctg gatataccct ataatggcaa
    29161 agaggtatgt agctatacag caactgtcaa tgtcactatc atggataaat ccgatcggga
    29221 ggcaagctat atagccgaag agatagcttc gcaatttatt gttttattat gccagcgatt
    29281 gcaggtgccg gcctatatat cgcataatac tgtgaaaagc gattttacca tcaatggaac
    29341 atttacctgc tataccgtgg agacactgca tgatgatttt gtttccctta aacctatgga
    29401 taacgttacc atagtcaaca atatcgttgc tggtaatggt gtatacgacg attgtatggt
    29461 actcattgat tttaaaaaag aagcagaagc catacaacaa ttttattatg gccgaaaaca
    29521 ggagattgtt tttaaaaaag cagcatggga tgatagctgt tctttaattg cttttactat
    29581 tccggcaagt gctaccatgc cggtggttgt accgtttttt ggatattatg ccagtgaaga
    29641 ttatgaagga ttgctgttat ggattggcaa tgttgtcccc tatactggaa ttcagatgtg
    29701 ggggtttacc cacaaacctg atacattgaa aaaacatcat gaggatatct cgcgtcacga
    29761 tagagctcta tactattttg gctggtatat gctctttgtt gggggcatgc cttcgtttgt
    29821 cgatgcattt tctcattcat accttgagca agcacggtac tataagggta atgtccccac
    29881 catgggcgat aaaaaaaatg aggtttacct gtcactgctt ggcggaggaa gcggcatgtt
    29941 ttataaggga tatcgagcat ggggatactg gtattttcat attaccaatc tttgtgttta
    30001 tggattactt tataattatg ccagaccgtc agaatgggac agtgcaaatc ataggtatag
    30061 aacacatgat gcaaataaaa gcgcatcata cgctttttta agcttgctgt ctgtcattaa
    30121 gatagttgag atggttcata cattatccgt gccgtatact atatcagttg tggagcagga
    30181 agatgcacgc attgaagtgt tgccggtttt tgcagtcaac gattatgaaa aaattacagg
    30241 tatagcatgc gcaatgcatt tttaatagta tggtgcatgg tatatagtgt tatcggcacg
    30301 gcagcctatg ctgatgatat accaataggt gttattatcg ttaataccag tgttgacacc
    30361 cccatgataa aagctctggg cagtattaca tcagaagcaa tcaaggaagc atttaaaaac
    30421 ggcaggcatc tggtgcccta tgatgcgcaa acatttttac agataacaaa cactaaaagt
    30481 gctgatgaaa ttgttgggaa ggctcatctg tatggtgttt cccttattgt gtatatgcag
    30541 gtatttcttg ttggacctgt ttacacctgc aacctgacat ttaaacctct tgaagctatg
    30601 agtactttta aagaagaaac cataagcata cgcagcacca tagcaaaaaa tattcctctg
    30661 aaagcggcaa aggaaatttt aaaacgccat tctgacgttt taccggtaac tatcaaacaa
    30721 aaaataaata actataccta tatcataaat gcagggcaat ggcatggatt acagcaggga
    30781 cggtatacgc ttacagatgg agctgaatgc gctattgtga gcgttaagcg ttatagcgct
    30841 ctggttacca tacatgccga ttacaacgaa gctcaacaaa ttaccatgag tgttggaaat
    30901 aaaattacta agcttattaa acgtattgat gctcagattc atgagaacat caatcaagaa
    30961 tattcgggta aaaaactgct gaaaggtgac agcgatggta agcgcgcgat agaggcaacc
    31021 tgtgttgtta atccttctgg caatatactg ctgccggggt atggagcatt cctttcaaca
    31081 cactatctgg gattttcgca cagcgaaccc gattggccgg ggatatatgt tgcgttaggt
    31141 gcggttgcaa cacagcttat gcttgtcccg gcacttggca actgggatgt caattttttc
    31201 ccatgggtta acggcaacaa aacaaatgct caataccgcc tgggcattta cctgtgggca
    31261 acgcttcctg tcacctacac ggttgctttt tttaatcagc tttcgtatgc gtaccagaaa
    31321 caaaaggttt tgccaccatt ttttttacat gatgatgtta cggcttttgt tgcttcggcg
    31381 atagttcctg gtggaggtct tttttataaa gggtacaggc tgtggggaca tgcttactgg
    31441 cttacggaat gtacgttagg cgggttgctg acctatcact gggaggatgg cggaaagcgg
    31501 gctgtttttg caggagcctt aatcgggata aagctcatag agctgattca tgcagtgctt
    31561 gcttcgccag catattcggt atatacctat gaactatcaa acaatgaaaa tgtgcatgtt
    31621 gatgttggta tggcaccgtt tcaaaacgaa ccatgtttct atgcatattt ttttaaaaat
    31681 tattgaatca cagcgagcgt gctatataat tgttaaacag ctaaaaacaa aggatgccta
    31741 tgcaaaaaag agcatattta ctgttagagg atggaaccat ttttgaaggg tatagctttg
    31801 gctatgaaaa agatgcaata ggcgaggctg tattcaacac atcgatgagc ggatatcagg
    31861 aggttttaac cgatccttca tatagtggac agatagttgc tatgacctat ccaatgattg
    31921 gtaattatgg tgttaacaat gacgatgtgg aatcttctaa agtgcaggtg gcaggatttg
    31981 tggtcaaaga gtacagcaag gcatattcca gtcaccgggc tgtgcgctca cttgatgaat
    32041 atcttaacga ttataaaata cccggcattg aaggaattga tacgcgcaga ttaacgcgcc
    32101 atattcgcga taaaggtgcc atgcgtgcag gaatatgtat ggacaccagt ggagccatgg
    32161 aaaggatttt agctcatcct gctatggcag gattggattt aacctgtaat gtgatgtgta
    32221 ataaaccgta ctcatttggt aaagatagaa gtgaagcgcc aaccattgcc gtatttgact
    32281 ttggtgtaaa gatgaatatt ctacggctgc tccatgaaca gggatgtaat gttgtggtgt
    32341 atcccgggaa tatggcgtta tcgcaggttt taaaggatag ggatatcaaa ggggtgtttt
    32401 tatccaatgg tcctggcgac cctgatgctg tggagtatgc aaaaatcctg gtaaaagata
    32461 taatgaaggc tcacataccc tgttttggga tatgtctggg acatcagctt atagcattgg
    32521 ggatgggagc aaaaacctat aagcttaagt ttggacaccg gggcacaaac cagccggtaa
    32581 agaatctttt aacacaaaag attgagatta catcgcagaa ccatggattt gctgttgatt
    32641 atgaatcaat aaaacacaac agcgatgtgg agataacgca tattaactgc aatgatacaa
    32701 ctgttgaggg attgcggcat aaaaagctgc cactattcag cgttcagtat catcccgagg
    32761 ctaatcccgg ccctcatgat tcgcgctacc tgtttcagca attttatgaa ttggtgacac
    32821 actagctgca aggtagagaa gcaatctatt catgagtgca aactttttca ggataatttt
    32881 tttatctgat gaatcatcga taaaaaatgt tattactgcg ttatagtaac aggatagtag
    32941 atagtaaaga ataatctttt tcatcttcct ttaacggcat gtacatacat tgcaaatgat
    33001 gatcattatt atactggatg ctttcatact tacatttttt atgatattgt atgggcgcag
    33061 gtacgggaga aatagtgtat tgtagcgcta ccaatgatgt gaataatgag aattctgatt
    33121 tttccatgtg cccataaaaa agaattgctt ctttcaaaat gattcacaag tttggtattt
    33181 gattaatgcc ggataattgt atatactcta tatagtattt attttttagt taaactactg
    33241 atggctggat tgtgaacaac catagagaat ctcatggtgg ggaaggattc cggaacatgt
    33301 ccggaatgac ggaatgacga gagcggcaaa tgtgttccgg gttaagcccg gaacgacgat
    33361 acagaaaacg tgaatatgga ttaagccagt aatgacaatg gcaaataatt atatgttcaa
    33421 tggaggtttt ttgtgagtac aaagatggat atctggaatg atatcgttat tgattcggat
    33481 agggaaccaa atcttacaaa caatgcacag attgtattgg agaaacgcta tctggcaaaa
    33541 gatgaagagg gtaaggtcat tgaaactcct cgccagatgt ttgagcgaat agcaaaatat
    33601 atagcatcgg ctgacttttt atacggaaga gacagcaagc atgtttcaca ggttgctaca
    33661 accttttacg atatgatggt tgaccttctt tttataccaa acagcccaac acttatgaat
    33721 gctggcaggc ctttaggaca actatcggca tgctttgtat tgcctgttga agattcaatg
    33781 gatgctatat ttgatgcaat aaaatatatg gcattgattc ataaaagcgg cgggggcaca
    33841 ggattttcat tttcaaaatt acggccaaaa aatgatgttg tgcaaacaac aaaaggtgtt
    33901 tcaagtgggc ctgtttcatt tatgaacata tttaatacag caactgaaac cataaagcag
    33961 ggtggtacgc gacggggcgc caacatggca atactgcatg ttacccatcc tgatatactg
    34021 gagtttataa aagcaaaaga aaagaatgac gcgcttacca atttcaactg ttcagttgcc
    34081 ctgagcgatg cctttatgaa tgcagtggaa aatgatgatg actatgagct catcaatcct
    34141 cgaaataaca gggttagcgg tacgcttaaa gctcgtgaag tttttaacct catcgtccgt
    34201 atggcatgga aaaatggtga accggggata gtatttatag acaggattaa tgaaaaaaat
    34261 gttttaaaaa aagtgggtct cattgaaagc accaacccct gtggggaaca accgcttctt
    34321 ccgtatgaat cgtgcaatct gggatcaatt aatgtagcgc gatgtgtggt aaacaatggc
    34381 aagtcggtac gtgtggatta cgaactgtta aacgatatta cctataaagc agttcatttt
    34441 ctggataatg tcattgatgt taacaactat ccgctgccaa taataggaga aaaaacaaaa
    34501 gcaaaccgca agataggact tggtatcatg ggctttgctg atatgctcat tgcgttaggc
    34561 ataccctatg attccgaagc tgcgctgggt gttgcaaaaa atattatgtc agccatccag
    34621 gatgcttcaa aagaggcttc gcgtgcactt gctaaagagc ggggagcatt tccaaacttc
    34681 ccgctttcag agtatgcaca caacggtgat gagccgctga ggaatgctac aacaacaact
    34741 atcgccccga caggtactat aagcatcatt gccggtacat caagcggggt tgaacccatc
    34801 tttgcactgg cgtatgtgcg caatgtcctt gataacagca gattgattga atcaaaccca
    34861 ttatttgtgg aatattgtaa agagcgtgga ttgtatactg atgagcttat gcaaaaggtt
    34921 gcgcaaaccg ggtcagtggc acatatcgaa gaactccctg aagatgtacg tgctttgttt
    34981 gtaacagcac atgatattgc accggagtgg catgttcgta ttcaggcagc gtttcaggaa
    35041 tatgttgata atgcagtttc caagacggta aacttccctc attcggcaac ggtaggagat
    35101 attgaaacgg tgtatatgct ggcatatcat ctgggatgca agggcataac tgtttatcgg
    35161 gatggttcac gtgaaacgca ggtgctgcag gttgaaaagg gaaagcagcc tgtgcctatt
    35221 atgttcaata ttcaaccacg cccaaggaaa gaggtaacat ggggtaaaac actgaagatg
    35281 aatacggggt gcggctcgct ctatgttacc attaatgaag atgagtatgg gttgtttgag
    35341 gtgtttgcaa ccatgggcaa ggcgggcgga tgcgcagcca gccaggccga ggcagtaagc
    35401 aggcttatct cgttaggatt gcgatcaggg attgaacctg agcaaatcat aaagcagttg
    35461 aaaggagtac gctgccccaa tcaggcatgg gttatgggtg gaaggatcta ctcatgtgcc
    35521 gatgccattg caaaggctat agagcgatac atacatcctg atgcagtaca ggagcagaac
    35581 gaggacatac acaaaaatat tgccgagacc aatggtaaag ggagcgatac ggtgatggtt
    35641 ggcgtgtgcc ctgaatgtca tggcccgctg gaatttgagt caggatgctc ggtatgccgt
    35701 atatgtggtt tttcacgatg tggttagcct atgtatcagg tttattttac tgctgatatt
    35761 gatgaacagt taagtgaaaa gttagcgctc tacgcgcaga gcatttcaat actgtatgat
    35821 gaaaaggcaa ttattgcgtt gtttgatgaa gaggatgaaa taccatcatt tgcaaaagat
    35881 tatattttac agataaacaa tttagaagaa gaggtctgga aataccggta tcttgattat
    35941 cttactggtg ttgcaataac gccatcaatc tatgtttatc ctaccacaaa agcattgggg
    36001 ggcgatagtt catatacata tactattgcc ttaaatccaa aggatgcctt tggtgatggc
    36061 acccatccca caacgcgcat gtgcattcag caactcgaaa aaattgtaaa tactttttct
    36121 ctggaagaac gacgatcgct tacactgtgt gatattggca ccggtaccgg catcatcgct
    36181 attgtagcgg caatgcttgg cataggaaca gttgaagcat ttgatatcga tacaacctcg
    36241 gtagagcgcg caaaggagaa tgctgcacaa aatggctgta acagaatatt gttttttgtt
    36301 tcagatattg caaatgttaa tacatataaa aaatataaaa ttattgttgc aaatttatta
    36361 tcagatatta ttgagaaaaa tgttaaaaac attgtatcat tggttgaaaa aggtggtaca
    36421 ataatactaa gcggtgttgg ccatcgctgg gttaaaggga taatacaatt atttcaacag
    36481 tgtggatgta caccaattca tgatgaaagt atgagcacct ggcattgcat ggtattgaaa
    36541 gcgaaataga tatgaagata aaaaacatta aggaagtata tcccggtata ttcagaattg
    36601 tagagtacag gtcactgaag gtaacgccac caattaacct ttatattata gctggcagtg
    36661 atggattgct gtttgatggc ggttatggtg atgataattc aatacagatt gttggcgatg
    36721 caattgatta cgtacatagc cttaatgaaa agtggcgcat cacgcgggta ctgttaagcc
    36781 atacccatcc agatcatttt gcaggattac gacgattgca gcagcagtac catttaaagg
    36841 taatgcttac ggaacctatg acgcatgtta tagcaaaccg gaaaagttat attcatgcca
    36901 ataaagagga tggagtatta aaaaagcggt attcaaccat taaacagcgg ttagcggcgc
    36961 tgcctctgga gtttttttac caaaaaatgt ttaaaaaatt gtacggcata cagtatgttc
    37021 ccaaccctga catcatcata tcaccgcagg atactataac agtagacaat gcaaaatgga
    37081 aaatcatacc agggccagga cattgcgccg atcatatcat gctttacaac aaaaagcagg
    37141 gcatactgtt tggcggcgat aatgttttgc gcagtatcta tacatggctt ggacctcccc
    37201 gctcggatct tcaggcatac atgcaaacct tacatacttg cttgcagctt cctcatttgc
    37261 aatgtatact tggtgcgcat ggtaaaccgg ttaccaatcc gcggcaacgc attgagcaaa
    37321 taatagaata ccgaaaatca cgcacaaagc agatttactc tatcattgca aatgctccaa
    37381 acggtgggat tacctttgat agattgatga atatgatgta tccgaagaag aacggacacc
    37441 gctggcttgg gcgcggctgg ataattgtaa ccattgaata tcttgtaaat aaaaatgccc
    37501 tgaggttcca ggataaaaag attatagcca acccggtaga tattaattcc gtgctgtgag
    37561 ttctttaccg gttaaaaatg caaaggcatc atgggcggtt acttcaggta tttcttccat
    37621 cgggatgtgt ccagcaccct cgtacacctt gaaggttgca tgcggcagtg ctttctgcca
    37681 tttagggaac tcagtagtat agggaatcca tatatctttt gtgccccaca tgaccatgat
    37741 gggacagtgt atatcagtta agccttttga aaggtcttcc cgttttgaat actctttcat
    37801 aaccataaag atgtctatcc atgcgctttt atttccttcc cgttgcgaaa gctcaaaata
    37861 tcggtcaacc acttcctttg ttaccttgct ctgatcacca tacacttcat gaacagcttt
    37921 attgataaaa tagcgaggca tcgcactgcg cgcaaatggg cgtaccaaag catttgttga
    37981 aaattttaaa agccacggca tttcctgcgg gaagccaact gaatcaatta agattagttt
    38041 ttcaactttt tggggatatt ttaacgcaaa ttgccatgcc atataaccgc caagcgaatt
    38101 ccctgcaatt gaaagctctg gtattgaaag ctgctcaagc attttgttaa taagttcaac
    38161 ataatactcg ggtgtataat tgccggatga aatgggcccg gttaaaccaa aacccggggc
    38221 atccacgcgg attattctgt aatcatcttt taatagagct acccagccgt cccaggtgtg
    38281 aagagaagcg caaaccccat gtaaaagcaa caaaacgggt gcatcctttt tcccttcatc
    38341 acgataatgg attgtaacat cctcaatcac gataaagcgt gaagaggcgc tggcatattt
    38401 tttctttaat tcatctacgg gtatgctgcg catccctaat gaggtgcagc ctggcaataa
    38461 cattaaaaag gcaaagagca atatccatgg gcgatagtta ctgtccatga taacctccga
    38521 taaaaatata ataatgataa caatataatt tcagaatgtt gtgcacatgt ataaatacaa
    38581 ccgacgaatc agtatttatg tgactgctgg aaaaagtcaa taataataaa agggttactc
    38641 aataaatttt tatcagtgaa taacatgtca ttcagaatga cgcagtactg aagctactat
    38701 tcgttcattc tcacaaaaga caatatcctt cgcctgtgtt cagaataata tgccactgcg
    38761 gtcattccgg gcttgacctg gaatctcaat aattaacctt agatcttgaa acaaggtaag
    38821 gacataattc aggacgactt tctgcgttta aggatgacac agtggaagat aaaaattaat
    38881 ttaaaaaatt ttttataaat tttgttaaat gacttgactt ttccataatc attgtattta
    38941 ttaataacta gaagagtaag ctttaggttt tttacaactc taagttctta ctgttctggc
    39001 cgcaagtaca cttgcggaaa gatgagtttg gtacacaatg taccacaggt ttaagtgtta
    39061 tggttttaca gaaaagaaag aggatgaagt tcctgaaatt tcatcctctt tcttttttat
    39121 tgcagctcgg aaacgtattg acagacacta agcgatgcta tagttgatta gtgggagcaa
    39181 ttagagaaaa gcaacatgac tgtataatgc ttattgataa atgatgaaaa tatccatgta
    39241 gtatgatatg gattcaacga aatcattatt accattatca gagtggggag atatttttgt
    39301 aaaaaccttt ggtttttaca tagtctgttg tttcaggttc taatgttgta tatatggatt
    39361 gcgggtcacg cccggaatga cgttgaggag taatcatatt ttcagaggag caggtaaagg
    39421 taattttatg aaaaggtcag gtatactata actctatatc gtcgataagg caatggtatg
    39481 taatcatgga tgagtgtatg ctttctataa aggttatccc aaaatcatca aagaatagca
    39541 tcgaatgcgt cgatggtgtg tatcgggttc atgtcaatgc tcctcctgtg gatgagaagg
    39601 caaataaatc attaattgca tttttagcaa aagtacttga tgtgccaaaa tcttcagtaa
    39661 ctatcgcaca tggtcaaaaa gcccgtaata aaaatgttaa aatttcagga cttgatgctg
    39721 aagcggtaaa attgaggatt gaaaattttg cacaacggaa agcccagcgg agatacgatg
    39781 aatagtaaaa acacatccaa tggtatttat tgcattgcaa ttatcctgtg ccttcactat
    39841 tgtgcctgct catcgggcag tacggtaacg gtgaaagaag ggtgcatcga taacaatgtg
    39901 ttgcgcgttg ttgtttatgt accgtattcc gaattgcccc aaaagaagct taccagcgat
    39961 gaactgttaa ccatagcttt gcaaaggtct gagcagcgat accagcaaat cattgttaac
    40021 gcgttgcagt taagcagtgg aaaacgtgat gaattgtccc cttcatatag caatcgcact
    40081 attgctttaa aacaaaagag agtagatgga taccttatta tggttgatta tgttctatac
    40141 gatgaaattg cgcaactttt acaatgcaga taaactttat tgtagtatgg cattgaaacc
    40201 tttaaaaaca gaggatagta cctgcacatt ttttgatgat gagtataatg aagccatgca
    40261 ttctctttcg ggtgcttaca gggaagcgct gtatatgcat gtgctcccat caaaggtgct
    40321 ggaaaaagaa ggtactgtgt gtgtgctgga tgttggtttg ggattgggat acaatgtgct
    40381 ggcgctgttg tatgaagcaa ggaaaagaaa tgccaatgct ttttatcata ttgtttcatt
    40441 tgaaaaaaat cccgctatac catgcaccat ggaaacaata atgtttcatg atgacagaga
    40501 tgaacggtat aagctcatca gaaaggcagc tgtagaaaaa gaggtaacca ctgcatggta
    40561 taatcatgtt gttttatacg gcgatgctcg taagcttatt gcatccatgc cagaagatta
    40621 ttttgatgct gtgttccatg atcccttctc accggcaaaa aatcctgaat tgtggacagt
    40681 ggattttttt aaaaagatat atcacgtatg cagggatgat ggtattgtta ctacctattc
    40741 agcggcattg catgtacgcg ctgcaatgca tcaggctggt tttagagtgg gcattggtcc
    40801 acaggtggga cgcaaaaaag aaggaactgt cgccacaaaa aaaaatgctg ctgttgttcc
    40861 attgggtgat gattatttta ctgcattaaa aactatcgct aaagctattc cctttactga
    40921 tgtgctgtta tgtgatgatc cggagttgat acggcagcga cgtcatgaag caatgcatgc
    40981 ctttaaaaaa ggtcatcaag ctcttgaata accgatgaag attctttttt ggtagaggta
    41041 tcccctggct ctgaggctga cgatgatttt tcattgtgct gtactatagc taacggtgtt
    41101 tcaccatctt taaatgcaac atcgcctgca agatcacctt cctgaagccg gtatgaaagg
    41161 gcacatgcgt aaaagtatgc aaggtgaata gcttcataca cctttttatc ctgtagcaga
    41221 tgtgctgatt ccacattgat atcaaactca agaaaatcct gtggattatc aa
//
LOCUS       DAOOJH010000016        39774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1114_length_39774_cov_9.414436, whole genome shotgun sequence.
ACCESSION   DAOOJH010000016 DAOOJH010000000
VERSION     DAOOJH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 39774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39774
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_39774_cov_9.414436"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..71
                     /locus_tag="PLJ75_03960"
     tRNA            1..71
                     /locus_tag="PLJ75_03960"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Gly,seq:tcc)
     gene            complement(79..843)
                     /gene="hisF"
                     /locus_tag="PLJ75_03965"
     CDS             complement(79..843)
                     /gene="hisF"
                     /locus_tag="PLJ75_03965"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPD04404.1"
                     /translation="MKTITIMPCLDMKEGRVVKGVNFINIKDAGDPVENAVYYQHEGA
                     DELAMLDIAATVENRKTRLEWVKNVSGVITMPLTVGGGIRSIEDIELVLEAGASKISM
                     NSAAVKNPDSIDEAAKRFGTEKITIAIDGKRNTAMPSGFEVVVAGGTISTGKDVVAWA
                     IECQSRGASVILPTSMDGDGTLQGYDIPFTRAIAEAVNIPVVASGGAGKLEHFYEAVV
                     EGKASILLAASVFHFRTFTIKQVKNYLRGRGIAVNL"
     gene            complement(930..2384)
                     /locus_tag="PLJ75_03970"
     CDS             complement(930..2384)
                     /locus_tag="PLJ75_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HPD04405.1"
                     /translation="MKRIVFIILLCFMSCSQKQYQDYIPNTAQCIYDESHNNSWRFFD
                     HGYCGYSTIAQFLKNYGIPPSITIKHLDECIQQCYRSGYTNTLLILSVAKYQQFDSGE
                     VDALCNFVKKGGLLFVIAEHENMYNSAQFLNAVTTKLGIQINNDAAGNGSTPLQSIDN
                     LNNSAYSKVFTLKNVLHMLAASLSRMNKTNSSFEILLCDGTTDKVIAAGCIYGKGRML
                     VVGDSEMFWNGDGKVGINAGDNRAFFKSCVEWLLKKRLNKIVHHTTNVRCFDPENSMV
                     QIDNSCSGIQCFMDALKEKIPSITTDSNITIVVHPQPQFTVKEGKRTIVFIEPYQNLM
                     SSTVWGNQLLKAGAVNPSVYVSFAKSAKIQILPAFITNGDSNYYMVTIAHDKNSLVFN
                     SLAALLSHRSDGMLFKIPSAFWGEVAHPGLEVINDGIPMYQSSDYTDAGYMYADSKLL
                     IVGDADAIANQNNNTQTFSAIVTMVTNWIQKGSL"
     gene            complement(2573..3937)
                     /locus_tag="PLJ75_03975"
     CDS             complement(2573..3937)
                     /locus_tag="PLJ75_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPD04406.1"
                     /translation="MAKDSTKIQLPEREMPRQWYNITADLPKPLDPPISPATGKPVTP
                     DELHAIFPMGLIEQEVSSKQYIDIPEEILHIYRLWRPSPLFRARRLEAALGTPATIYY
                     KHGGVSPAGSHKLNTAVAQAYFNKKEGVKKLSTETGAGQWGSALALAGNFFGIDVHVY
                     MVRVSYEQKPYRKFMMETWGAKVTPSPSNITNSGREILKQDPESPGSLGIAISEAVEE
                     AAQSKDTNYSLGSVLNHVCLHQTIIGLETLKQFEMVDDYPDVVIGCVGGGSNFSGLAF
                     PFVRDKINGKDINIIAVEPSACPTLTKGIYTYDFGDVAKLTPIMPMYTLGHSFIPPSI
                     HAGGLRYHGMSPLVSYLYHEGLISAMALHQNECFEAAILFSKTEGIIPAPESSHAIRA
                     AIIEAQKAKEEGKKKTIVFNLSGHGHFDLASYDKFLSGQLEDYDYPRELIDKALHDLP
                     KVVK"
     gene            complement(4226..5638)
                     /locus_tag="PLJ75_03980"
     CDS             complement(4226..5638)
                     /locus_tag="PLJ75_03980"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD04407.1"
                     /translation="MVTIFKKQNLRIHMIGICGVGMSALAGMLQSQGHMVTGSDTDVY
                     PPISTQLEKLQIPVCKGYSSDTPGKIKPDIVIIGNAISRGNPEVELVLNNRIPYFSMA
                     GALYNFFLYDKEVIGVAGTHGKTTTTSLLAYILEQADMNPSFFIGGIPKNFETNYKVG
                     SGRYFVIEADEYDSAFFEKMPKFIMYRPVHCIMTSLEFDHADIYNNIDEIAVWFTRLR
                     NLIPANGYIVYNNAYSNLSTINNDALSSTVSYGKMSADCCYRYQNEVLTITMDDMAIN
                     VTPHIYGDFNHQNITAAVAMALTLQVPVGAIVMAINSFQGVKRRQEILFSNDYLTIIE
                     DFAHHPTAISNVLTQVSEHYSDAAIVAIYEPRSATSRRNVFQDELPLAFSKAHKVIMK
                     RPYRLDKVPAHEQIDIDRVINKLCQMQIPACVLGSVNEIVQEAFSGLSNKRTVMVVMS
                     NGGFDGIYELLLAHTKNVFA"
     gene            5854..6513
                     /locus_tag="PLJ75_03985"
     CDS             5854..6513
                     /locus_tag="PLJ75_03985"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD04408.1"
                     /translation="MKSNIKSKDYQNFLDDLKGYYQENEQKEEENIPVGTRISQIRQM
                     QGVDSATLAKNAGIDHKYLEDVESLAIYPDLGTIIKITNALKVSTGSIFDLDSSRSYT
                     IIRKIDRKKIMRSISGVKEHPDYEYLSLSSGVISRHMEAFIVTLKSNVYYTEELSTHE
                     GEEFLYMLEGAMKIKLDDKEETLQQGDSIFYHSTIPHVLKSISPKPAILLAVLYTGYS
                     S"
     gene            complement(6600..7184)
                     /locus_tag="PLJ75_03990"
     CDS             complement(6600..7184)
                     /locus_tag="PLJ75_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04409.1"
                     /translation="MNYRPQDVLHLQLAHERGYGSLQLKDINITGDISIDKLRAKPKG
                     QRKLFQVLQELDTPLTFYSGYAPNTDIICDGGCEAAIKGCLGTIEKRRPGSLKKAKKG
                     AIVTGIYKGDIVVPDGNVLLVGDCTKVDGKLVAKRVMRIKGCPIGARNLFIPVPLLFG
                     LPSPMFDIRDAFLFIVNSIQKVFNIVKYRWVLKQ"
     gene            complement(7181..7687)
                     /locus_tag="PLJ75_03995"
     CDS             complement(7181..7687)
                     /locus_tag="PLJ75_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD04410.1"
                     /translation="MKKPHVIVRRCDDYDMSAISAIINESVNDLKLKIPIQENVFIKP
                     NVVTANKQYIFNSYTHPAVVEAMVTVLKDKSINNIIIGESGGYGIPSRLFLKEAGYFD
                     LAEKLNVPVVDLNEHPVVKVKLSKAKWHKEIELSKYIAEANFKIWMPKLKYYPIIHWL
                     VMWLHRIL"
     gene            complement(7700..9364)
                     /locus_tag="PLJ75_04000"
     CDS             complement(7700..9364)
                     /locus_tag="PLJ75_04000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04411.1"
                     /translation="MSFEVPVSLVQFLLPPMCFGLLIGGFILYVYMYWKYKNSLYFAI
                     AFFALLGMLFVGSETMILSFGGWLHNRSVSVHFHRLEQTAGLFYLFALPYSLNKLLIL
                     NKRFFKINTILAYIGLAYAVIATIGAFVFPDLFISLTKHKATWMYYEADYGRGQEGIL
                     YAIRDVLLGIYIIYSIVAVMIDMILHRNYRYLLYPALGLLFAFAGAAIDTLHVHTQVF
                     YDLFPNVYFSRFSVGITLAIMFFMSSVTSMFINYAQELEIAHKIISISEKKYRFLVEG
                     TNDCILSFDSNLNITTANRAAYEQLAVRRGLESATSFYDLVYLPEGESDLTLQIIKEK
                     INMLFTEGNPINLKIQLKSRGTLEPKEYTIRFEHIVIDDRNEILMKATSVLDENILKY
                     IEAEKLKLSIGNYLIAAEEICNRLVLNLPKYLDKKTTNTIKLGLREIIINAIEHGNLN
                     IGFEEKTQATNNGNYFEFVLSRQKDPNYKDKKVTIELLLNCNKVMYKIEDEGNGFNYR
                     DIIQKIQNTVDDDMLAHGRGLRMVYNIFDEVKFNNKGNQVLLVKNF"
     gene            complement(9392..9850)
                     /locus_tag="PLJ75_04005"
     CDS             complement(9392..9850)
                     /locus_tag="PLJ75_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04412.1"
                     /translation="MKRIVGVVAVVVVVFVFIAADKTPSAEELIDVLGLRHSKVFCKY
                     GVPVKMYVDRGPTKEEDYIIFDYLQFGLVFHHEMIRQILFYPEWKGGKIRKIDIGMSK
                     DEVIKIMGEPQEEVALSDGRTRITYLEPDKYFDVILSTSNVVNMIRFEVK"
     gene            10262..10975
                     /locus_tag="PLJ75_04010"
     CDS             10262..10975
                     /locus_tag="PLJ75_04010"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPD04413.1"
                     /translation="MRKRKSSPWVNNPTNELNDTGLTANMEDYMENIALLLNENDVVR
                     VKDIAQKLNITMPSVTAALQKLKEKGLVDYEKYGHVQLTPRGKEIADSVYHKHSFLSE
                     FFQSILNMDKKSAEEQACKLEHHLSNDASTRMQQFVEFYKHEQQANEQWIKRLDNTLE
                     ERRLSQLKEGQKAEIVAILGSGPLKKRLLEMGFRKGEIIHIIKYAPLHDPIEIEIKDF
                     LISIRVEEANYIIVKPIDE"
     gene            10982..13159
                     /gene="feoB"
                     /locus_tag="PLJ75_04015"
     CDS             10982..13159
                     /gene="feoB"
                     /locus_tag="PLJ75_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPD04414.1"
                     /translation="MTKKTITVGLIGNPNCGKTTIFNALTGARQKVANWAGVTVERRE
                     GFFNHKGYTIKVVDLPGAYSLTSFSMEELITRNFIQNDKPDFIINVIDAGNVERNLYL
                     TTQLIDIEAPVIIVLNMIDEAFDKGLIINIQKLSQLFNMPVIPTVGTKGTGIPQLKDS
                     IVENFEKQTKNHTITIPYGNDIEQAIQQLQQLIDKNRLEYPSRWLAINLIEGDIDAEK
                     RVKLLPDGNEIIQKAYTIRNNLSKAFHDDIQTVFADMRYGFITGALKEAVQKKPIKRH
                     DLTKKIDSVVLNRFLGFPLLFVILFILFNLTFILGEYPMKLIEAGVEWLSHYLMEIIP
                     PGELQNCIVQGIVGGVGSVIVFLPNILILFLGISFMEDTGYMARSAFLMDKIMHSMGL
                     HGKSFIPLVMGFGCNVPAIMATRTLENKRDRILTVLINPFMSCSARLPVYILFAGTFF
                     PNHAGFIILFIYALGIILAFTSAKLFNNTILKGKSTPFVMELPPYRMPTTKTIVIHMW
                     DRASSYLRKMGGIILAFSIIIWVLSEYPKPYHIQKQFDTMIQQKIIELNQTLSTYQKQ
                     NADPNTIQTIHKQYNTIIEDLEIQKRQEMVQYTFIGRIGSFIYPLLQPLGFNWQMGIS
                     LTTGFVAKEIVVSTMGVLYHAANDENTVSLSQKLGNPRYGITPHAALAFMIFVMIYIP
                     CLATVIAIAREIGTRWAFFSVAYQITVAWIVSFIVYQIARFIL"
     gene            complement(13173..13364)
                     /locus_tag="PLJ75_04020"
     CDS             complement(13173..13364)
                     /locus_tag="PLJ75_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04415.1"
                     /translation="MNDNTNKGFRYYIDDATLNGYQQKPYHLRLQWLYMGNKLRKGYS
                     STIKAIQDSFRRGEGSFKQ"
     gene            complement(13386..>13601)
                     /locus_tag="PLJ75_04025"
     CDS             complement(13386..>13601)
                     /locus_tag="PLJ75_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04416.1"
                     /translation="IKNKSMKAFCFANPDWAISKIDIIIDSPINYEEAITRAKFVTIN
                     GIKLPIISIDDLITMKKVSMRDQDIVI"
     assembly_gap    13602..13701
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13966..17025
                     /locus_tag="PLJ75_04030"
     CDS             13966..17025
                     /locus_tag="PLJ75_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04417.1"
                     /translation="MNSDINFTENADHVKYLLQIANLDTTSSLSEYVHNLKTTIAQLK
                     IPVFNISCLSNIEGTDHLLIFPDWDYLKQWVPELSISNNIFEYATINIKKLIANRAIF
                     KKSILHSLLRGYLTLTDTISIPEVIQYHKAIVSCEQMISDFSQASKELFQILNPDVQN
                     RILESPLFGSAISFPLFSDRDVIGSFDVVYPKNYPEINDNEFATLELIAKLIASGLRQ
                     YRYQNKIIISETKYSSLVNAIPMALFVINKEYSIIETNDTFKKWFNIERPLNKKCYEI
                     VGCSNKTCNGCPASKAMDKAAPILQEKKVPYFKDTRFFNYITSPIIAPSGSISSVVVI
                     MEDITTRINAEKKIEKFSLTLAEEVDNQTKQLKEKQQKLQLTTNTLYALKQANTIKES
                     IHCIIESLHTLGCKYCIVTLLSSDVNTLQIVEVYPASFLQLLQAVTNQNITTIKFDFD
                     DSANIPFFSVIKSGKEIMIEHFSELISFLESTFPQIESDKIKQISSLLKDEVIGIYPL
                     GSKHQIEGSIAIAFDKQSIEENKDYIEILLNAAAVEINRKRNQEELVKSELKYRNLVE
                     NTQDIIMLCTAEGEIIYGNSAFYKLLAYPETRKPSFFNFIDDNEKYLIQSIMQHLLNY
                     GINPDPFEIQMYTCDEKFLWFEIAMSMIPLDHTVGIQIVARDIERKKRMESHIQNLTE
                     FQEKILQNEMIGIITTDLSGIITSWNKAATSILQYQPFETLNTPLQNLILNYYEDSVL
                     SKSNTFASIINQPYTEIKMLKKGGTIITTLCMSSLLHDDHDMPFARLHFFIDISEKKQ
                     LEAESLELNKKLQHAQLVTIVSLAKLAEYRNKETGMHLERIMRYVEILARELSQHPDY
                     RDYITEEYIQDLVNSCLLHDIGKVGVPDHILLKPGKLTDYEYEIMKTHTIIGAETIAE
                     AEKKVEGRSYLNLGKEIAYYHHERWDGSGYPKGLKGDAIPFSARIVAIADVYDALVSE
                     RPYKRAFPHEKAVEIISKKAGTYFDPVIVEAFIKHHNRFLQIKETLL"
     gene            complement(17059..17514)
                     /locus_tag="PLJ75_04035"
     CDS             complement(17059..17514)
                     /locus_tag="PLJ75_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPD04418.1"
                     /translation="MKVLVISDTHGNIDAIKTIIEAERKFDYLLHCGDGINDLMYVSV
                     PEGVFVITVCGNIDRARGINEKSTIVETIGNFKFFITHGDVYGAHHDITGVWIEAKHS
                     GCDIACFGHTHKPCNTNGSPMMFNPGAAQSGFYGIIESGDSLKAVLKRI"
     gene            17667..19463
                     /gene="lepA"
                     /locus_tag="PLJ75_04040"
     CDS             17667..19463
                     /gene="lepA"
                     /locus_tag="PLJ75_04040"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPD04419.1"
                     /translation="MEQSLIRNFSIIAHIDHGKSTLADRIIERCKAIDPRNHVDQLLD
                     DMDIERERGITIKANVITLNYQSKDGKIYTFNLIDTPGHVDFAYEVSRALAACEGVLL
                     LVDATQGVEAQTIANMYLALENDLTILPVINKIDMPNADIQRTKESIKKSLGLDPDTV
                     LCVSAREGIGIDELLEKIIEIIPAPKGNPSAPLKALIFDSYFDQYVGVVTKVRIFDGQ
                     VQEDDEIMLFSTQKQYEVTEVGHFRLKREKRVQLTAGEVGYITAGIKQLTDTRTGDTI
                     TSAENPVSHPIIGFKEAKPMVFSGLYPSISDDYDDLKDALYKLKLNDAALHFEPDNSY
                     ALGFGFRCGYLGLLHMEIVQERLEREFNLALITTAPTVEYRVIKTTGEVLLIDNPVKM
                     PDPSQIERIEEPFVQATIITPSEFIGNIMALVNECRGIHKKMEYIDPTRVELFYEMPL
                     AEIMFNFYDKLKSCTRGYASFDYELIDYRASDIVKVDILVNEESVDALSFMVHGDKAY
                     KRGREIIERLKEVIPRQQFKIPLQAAIGSRIIARENISSLRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRLKQIGSVEIPQEAFLTILKIE"
     gene            complement(19481..22033)
                     /gene="glgP"
                     /locus_tag="PLJ75_04045"
     CDS             complement(19481..22033)
                     /gene="glgP"
                     /locus_tag="PLJ75_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010043392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPD04420.1"
                     /translation="MGTIQTFRVYPYLPDNLKPLKTIAYNLWWVWNFEAIELFRRLNV
                     ELWRDLDHNPVALLGMLSQSELDEAADSESFMAHLKRVSEELEWHLTKKSWVEDAYPT
                     YKNMQIAYFSAEFGIHECLPIYSGGLGVLAGDHIKSASELGLPFYAVGLLYRLGYFHQ
                     YLNIDGWQQERYPETDFHNLPLQLILKDDKPLVVEVEYPGRIVFAQIWECNIGRVKLY
                     LLDTNITQNNLEDRTITDQLYGGDVEMRIKQEMLLGIGGYRLLKALGKNISVYHMNEG
                     HSAFLSIERIHDTMHTHKLNFYEAIEYVNASTVFTTHTPVPAGNDRFVPELIDKYLSH
                     YFQKLSISRDEFLALGRENPHDKIETFCMTVLAIKTAAHCNGVSKLHGSVSRRMWKNI
                     WPGVPVNEVPIYHVTNGVQILSWTSDEMMRLLNRYLGPRWIDNPVDKTIWKGIDTIPD
                     SELWRCRERLRERVVAFARKKLREQLIARGAPRAEIEKADSVLDSEALTIGFARRFAT
                     YKRATLILKDMQRLERILLNKEKPVQIIFSGKAHPRDNMGKELIKRIIHLGRDDRFRS
                     KIVFLEDYDINIARYIVQGVDVWLNTPIRPQEASGTSGMKVVPNGALNISVLDGWWPE
                     AFNGNNGWAIGKGEEYHDPDYQDEVESLSLYNILEQEVIPLFYNRGADGIPREWANRI
                     KESMKTILPQFNTNRMVKEYTEMYYLTAHDNFVKFSKDDFKASRSLAKWKKDIMQKWH
                     QVTLESITYESKKEIIVGSQLKAKAVVNLGDLKPTDVQVELYFGNLNQKGEIEDGAAL
                     PMHMVEESGGGKYIYEGRMLCLKSGQFGFTVRVIPLHSDMPRKYESRLITWA"
     gene            22257..22970
                     /locus_tag="PLJ75_04050"
     CDS             22257..22970
                     /locus_tag="PLJ75_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPD04421.1"
                     /translation="MDFTIVLYQYPLGSFITHHTFQNLQRIKPHFICFPEYFFVNKKL
                     GNHAQTYHNQQLQLKRIQLLSRELDTVVIGGTMPELNGNKLHNTTFVFHNGTILGFYR
                     KQNLFFAEEGKITPGNGHVIFNAYGIRFSVLICADVFKDENFIALKEQGVQIIFIPTF
                     SLFRNETIDEKYKRDNDIFIRGAKLADAIIVKVCGVKSDYKNFLQARSLIASPEGILY
                     RVEPEQEDKSMLIVNTITI"
     gene            complement(23050..23916)
                     /gene="speE"
                     /locus_tag="PLJ75_04055"
     CDS             complement(23050..23916)
                     /gene="speE"
                     /locus_tag="PLJ75_04055"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HPD04422.1"
                     /translation="MPFESNVWIDEIYEVESGRTMKLKVIDKLVTYDSKFQKIEIYET
                     KAFGRLLVLDGVFMTTELDEMGYHEMIAHVPMCVHPAPKRVLVIGGGDGGTLREVLKH
                     KEVEKADICEIDGDVIRLCKEYMPALSCSFDDPRVTVYTEDGAKFIAERKNAYDIILV
                     DSSDPIGPAAVLFSEQFYKNVSQCLDSDGIASTQSESIFYHSNIIVTLANYNKKYFKV
                     PSYYYTMVPTYPSGMIGFSFCSKKYHPLNDFIQERAQVLLPQLQYYNPDIHRGAFALP
                     QFIKSKLTGIIV"
     gene            complement(24218..24568)
                     /locus_tag="PLJ75_04060"
                     /pseudo
     CDS             complement(24218..24568)
                     /locus_tag="PLJ75_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770061.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     gene            complement(24615..25022)
                     /gene="speD"
                     /locus_tag="PLJ75_04065"
     CDS             complement(24615..25022)
                     /gene="speD"
                     /locus_tag="PLJ75_04065"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HPD04423.1"
                     /translation="MEGLGTHIIAELFNCNAFHINNLKKVEEVLTAAAELSKATIIQP
                     FFHKFSPYGISGVIVIAESHITIHTWPEYGYAAVDVFTCGDHDYKNAIDYITEKLEAD
                     RCVLYEMRRGVMNDSKVKCIPVIREVENVNYIE"
     gene            25386..25458
                     /locus_tag="PLJ75_04070"
     tRNA            25386..25458
                     /locus_tag="PLJ75_04070"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25419..25421,aa:Met,seq:cat)
     gene            25463..25536
                     /locus_tag="PLJ75_04075"
     tRNA            25463..25536
                     /locus_tag="PLJ75_04075"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25497..25499,aa:Met,seq:cat)
     gene            complement(25534..25920)
                     /locus_tag="PLJ75_04080"
     CDS             complement(25534..25920)
                     /locus_tag="PLJ75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04424.1"
                     /translation="MKNKYTLKKIVIIEFIVFLLLVMFFWFEEILDLPYIIFNEEPTP
                     INFVECLMETVVITILFGILLYETIKINLSMGKLESYIYVCARCHKIFINGEWISLDK
                     YFNEYANKKTSHGLCDVCKRNYTKED"
     gene            complement(25930..27075)
                     /locus_tag="PLJ75_04085"
     CDS             complement(25930..27075)
                     /locus_tag="PLJ75_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD04425.1"
                     /translation="MEVILKECKTYKDDIVTKQITSALNALHFDFSRFYNAKVAIKPN
                     LLTSTAPEAAVVTHPLFFRAIVHVIKQHGGIPILVESPAVHSLQRVMKKAGYDAIVAD
                     EEIQVADTSDYIIIHNENARHFKYFEVPKIFTECDIIINLPKFKTHALTYITCAVKNL
                     FGTIHGMKKSQWHIKAKTKSEFSEMLLDLYEAYITDSSLPKTIIHIVDAITILEGDGP
                     GLKGNPKFMGIIGASYNAIAVDYALSKIAGFTIDEIPTITMGMKRNICAVSDAITIVK
                     DAAIENNYNCIPPKESGSQSILSISIVNRLLKNLIIAKPLPDDNKCTLCYQCKQICPA
                     KAISIAKDGETIPNYNYSKCIRCYCCMEICPESAISLSQPWLQKLMR"
     gene            complement(27114..28265)
                     /locus_tag="PLJ75_04090"
     CDS             complement(27114..28265)
                     /locus_tag="PLJ75_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04426.1"
                     /translation="MKSNIITKEAYKAFKKGETSVVIQLLEKNIAISNDPYDFFLLVI
                     AYLLTDKLVQAEATLRKALRQHQNYIPLLEIKAFLILKAAKTKDDACSGYVDIIKLSP
                     NNKKITKIIKTVHETDNFEKFQKQARIKDFITIQPPKKHAMHKHQHSGRARFPVMKCV
                     LLFSIIAIIIAGIIFFSSPLAKLITQKISLLFTETRSDIHELSNITLDGMHYDVIDTI
                     QKFKTPEFYTSGEECIRDFNHAKWLIKEGKENDAMIILNKIDASNVQMKVKQRVAFLK
                     DFIIRNRERTVSGYLYNDVIKKPYLYKGINVKWRGKVANYKKQGRNHTFQLLINYEND
                     RFDGVVEVFSSTADNSITNGTIANVEGIITNIVAQKIIVLHALNIEIVR"
     gene            complement(28321..28884)
                     /gene="efp"
                     /locus_tag="PLJ75_04095"
     CDS             complement(28321..28884)
                     /gene="efp"
                     /locus_tag="PLJ75_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD04427.1"
                     /translation="MITSNDLRNGTVIKVDGDLYSVVDYQHVKLGRGGAFVQTKLKSI
                     TKGTIVDKKFKGGEKVEDVRIEKKTLQFLYDEGDALIFMDTETYDQLSIDKKKAGNLL
                     NYIKEGDNVEATMHNDAILSIEPPIFVVLKVTYAEPGVKGDTATNVLKPITVETGATI
                     QVPIFVNEGDTIKVDTRKGEYVERVKR"
     gene            complement(29115..30191)
                     /locus_tag="PLJ75_04100"
     CDS             complement(29115..30191)
                     /locus_tag="PLJ75_04100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPD04428.1"
                     /translation="MNYLSRIKKIRNKLKNNDCFPYLVSSLINIKYCTGFDGSYGYLI
                     FFPDETVFISDSRYEEYAISILPQNVRFVLQEKDINQTLSALLKKKSVKKLFLEEHAI
                     SLSQYKAMKEHCRGIALEYGGDSINELRVIKEEEEIEIIKQAAKLTDLCFNHILNILK
                     PGITEWDVATEIEYFYKKNGCLGTSFATIVASGNGSSMPHYKTDVKIIKEGDPVLIDM
                     GCIYNGYCSDITRTVFVHSASEDIKNIYVIVKKAQENAIAAVKPGMTTNKLDSIARDF
                     IAAHGYADKFGHSLGHGVGMDVHEIPAIKQNGMIKLKKGMVITIEPGIYLPKRGGVRI
                     EDMVLITNNGYEVLTKSPKDIIVL"
     gene            complement(30202..30651)
                     /gene="aroQ"
                     /locus_tag="PLJ75_04105"
     CDS             complement(30202..30651)
                     /gene="aroQ"
                     /locus_tag="PLJ75_04105"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPD04429.1"
                     /translation="MKQYTFHVVHGPNLNMLGTREPGIYGVFTLDEINNKIIEYAKSH
                     NITIHCFQTNSEGEIIDYIQRNKNCDGIMINPGGYTHTSVAIRDCIAGCNTPVIEVHL
                     SNIHAREHFRKHSYIAPVCIGQICGFGYYSYILGIEALVYYLNNIQK"
     gene            complement(30714..33536)
                     /locus_tag="PLJ75_04110"
     CDS             complement(30714..33536)
                     /locus_tag="PLJ75_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04430.1"
                     /translation="MKRRVIVLVILLHSIVCSLAFALESSQLFEQGMEAFRMGNYGSS
                     ELIFRKIIDANDSYKDRAWYYLALSIFYQKQYRSAIFELNRFLLLCTTPDLCNEARYW
                     IAESYYYLNDYIKAIEEYNRFITQNKNKQLIINSYDRIGEIYFNQKRYDEAIIEWRKA
                     LTLTSKKFEDSNRVIKIGEALFLSERYSEALDILEPLIHAVNDATISSKAQLIVGRIY
                     QIQGKHWQAIKYLNEIPESMIKIPAFLSVQYFKALSYIALNDIYNARLNLKSYILISN
                     EDSPYYYNARYELGKILLNTKDLDEGISLLADVQVNSNDIDLRSRASFELGQLYLKNN
                     TIEKAIPYLENAISINDQESQKYVMMTLSELYIATDRYDDAKRLLDFMLEKYPYEENR
                     DEMLYLEAKIYILQGDVEKSLEYFELIKEVNPFSKYLVELDYAQAVDFFKRGDYTSAI
                     ASCNRYLNNYKVDNRYEVLIILSRSYIKINNFKKAVETINQLIAKFPKKNGLDEILYE
                     LSLSLNEKGVNVEKYWNFIIKNYYSSITAGKIYIEKGDEAYKKKNYQSASYFYSQYLS
                     IKGRPRERSVLLFYIISLYELKQYNTIIKTLKDSEYIAVDDYTTKLLIKWLAKSYYML
                     KDYTIALNTFMKLPLMDYSMDDLVLISDTALKNNQPDIAIETLQYMEQDNRYNEVAYN
                     IALYYKNNGNIDLATEYFKKVLIKKHHGMVLSNAQIQLAQIYLEQEQFDNALELVKNI
                     QDKENMIMRDSIAFIAHYKLGNINEALSIINNKLNDINMTAFADEIFTLLLQHYYKQN
                     DIKNLKIIYSYSSKRKKLISVSNYYYGLIHYINNNYNQAINYFTIASRFQDEYGISSR
                     YYLGIIYFAIQKNFVTAKRNFDRYFEKAEQDDINYYNAKIYVSMLLFETGKKDESKTI
                     LYEIVNNEGNILAKIKATHLIKHYGF"
     gene            complement(33539..34759)
                     /locus_tag="PLJ75_04115"
     CDS             complement(33539..34759)
                     /locus_tag="PLJ75_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPD04431.1"
                     /translation="MTKVALIATGSELVNGRIHESNSYYISRQLFSIGLPVVAHYVVG
                     DDSASLRSIIEHAMYSADIVFITGGLGPTVDDITCETLQSMFHLNLTVHKPSKARMDY
                     FFKATGKSALDTDVKMVTVPENAYVFPNENGLAPGFAIGYDNKDIIVLPGVPHELQSM
                     MMSVIEYCKKKYQLQKKEELTFKVIMMREAEVDSAVKEIVNKQENIDIAITYESGTTT
                     VILSPKSGNVLPAVDIVDEIKKRFGSKLIFGYNSLEEKVVHLLAKNKLRVAVAESCTG
                     GLISKRITDIPGASSIFAGGVIAYSNEAKKTLLGVKVDTLNRFGAVSRETALEMAWGV
                     KQRFASDFGIATTGIAGPDGGTKEKPVGLVCFGIAGSDTFSTQIQFSGNREHVRTLAA
                     NYILNKLLTFAGIE"
     gene            complement(34843..36510)
                     /gene="argS"
                     /locus_tag="PLJ75_04120"
     CDS             complement(34843..36510)
                     /gene="argS"
                     /locus_tag="PLJ75_04120"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPD04432.1"
                     /translation="MIIKEYIAQIVDKALQKVLQAHDILQQPSLSIKIEYPKEEKFGD
                     YATPIALECARILKTSPLQIGEQIKGFIDEQHIFERIDVVKPGFLNMFLSLPFLVQRL
                     QAIVTANDDYGRNKKEYPRKVNIEFVSANPTGPLNVVSARAAAVGDTLANLLAASGDT
                     VNREFYVNDYGNQVWWLGKSVWVRYQQMLGKDVIFPDEGYHGEYIKDIAKYIHENFSH
                     ELSNINDEEKAVQFCARKAVDYNIAWQKQDLERFNVHFDTWFYESTLHESGKVLDVFN
                     YLESLGVITDDNGKKVFVSTKFGDDKDRVVIRDDGRPTYLMADIAYHRTKIERGFDLI
                     IDIWGPDHHGYIARLVGAMKAMGYDENQFRILISQQVNLLTNGEAVKMSKRLGTFSTM
                     HDLIDDIGTDVARYFFVMRSMDSHLDFDIDLAKRQSSENPVFYLQYAHARICSIFREA
                     QNRNINYDKIIVDTAHYSNPETITLLKLMARFPEEIYDAANSYEPHRITVYCMRLAQA
                     YHKFYTEHRVLSDDHDRTQSYLILCDGVRIILKNALSLLGVCAPERM"
     gene            36733..38721
                     /locus_tag="PLJ75_04125"
     CDS             36733..38721
                     /locus_tag="PLJ75_04125"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD04433.1"
                     /translation="MQINKSYLLGLGLSIAVFLIISFFYAQTKLLDGLEYGSINFRFF
                     LRDPSEKAIKLQEGVKMTRKNPRSRDDIIILGIDENTIREFDNEGIKWPFPWLYHAKF
                     TQYVASGNPVAIFFDIMFLDHKPYEDEFAKAIKQANCVFLDYPFETEEVDVKYSDIEE
                     RMNLLNIYAMTIDPNDNSMPWVEEAVPPTPLLSQAAIGLGFANIKPDSDNINRKVPLV
                     VKYKGLYYPSIDLLLVMHYYGISKKDVEIKVGQYIKLSNIPKEKMVKTTHDNSIKIPI
                     DAEGFMDINFIGGPGSFTHYPYYYFCRDGIITNTSLQDKIILVAAYSSTGISTDIHKS
                     PYGDLFGIEHHANALNTILNQDFIVKLTPFQNILILFAIAILLGFILTRVSIIISVLF
                     TVGLSLLYIIGSYLLFDAFNIITIFASPIIQVGTTFSIIIAYRVLTEQQEKKYIRQTF
                     SKFVSKSVVDELLKNPENLKLGGEKKFLTVLFSDIRSFTTISEKMTPEELVEHLNEYL
                     QAMTELVFKYDGTLDKYVGDEIMAFWGAPIPQDNHALMACKCAVEMIQVLEQLNKRWI
                     QMNKPALHIGIGINTGEMVVGNMGSASRMDYTLMGDNVNLGARLEGTNKQYQTNIIIS
                     EFTYEQVKDYIIARELDLVRVKGKQLPVKIYELIDIKQ"
     gene            complement(38701..>39774)
                     /locus_tag="PLJ75_04130"
     CDS             complement(38701..>39774)
                     /locus_tag="PLJ75_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPD04434.1"
                     /translation="EDIAIFSKTKNNAMTYIKDSWLKINGDIKKININACAKHQKSDV
                     MLRSTINIEKWVPFTSTDEIMVQSKRLSAELLSKLLLKGIKELYSKAAEDLSIGYNEI
                     YFRQKPLGKYLSNNIINTKINVDTLTFEKNAHLKNINIELLSNKGLVTTQSFNCEGYS
                     ANYNFSVNAFCNSDYPTMTISCAVNNFDYGTFLRDTGLKDATGLMNMNLNYGVSGYRL
                     SHLLQNGNGNFTLNIANSVLNSSMIQRKIIELAKSCNIVVEGGGWHCNRIDVAINHTA
                     DRLILQSLYIDTDNASVSGYGNYSIENGLSLPCNATFYKRDGRTITTTHRASFSIKGE
                     LLRPFIVSSFPCGKEQILLFDVN"
BASE COUNT        12814 a         7693 c         6163 g        13004 t
ORIGIN      
        1 gcgggtgtag ttcaatggta gaacttcagc cttccaagct gatagcgtgg gttcgattcc
       61 catcacccgc tcatttattt acaaatttac agctattccc cttcccctta agtaattttt
      121 cacctgcttt attgtgaaag tcctgaaatg aaacactgat gctgcaagca aaatgctggc
      181 ttttccttca accactgctt cataaaaatg ttctaatttg cctgcaccac cagaagcaac
      241 caccggaata ttcacagctt cagcaatagc tcgtgtaaat ggtatatcat acccctgtaa
      301 tgttccatca ccatccatac tggtgggtaa aataacgctg gcaccacgcg attgacattc
      361 aatagcccat gctacaacat cctttccggt agatatggta ccacccgcaa caactacctc
      421 aaaacctgaa ggcatagcgg tatttcgctt accatcaatc gctattgtta ttttttctgt
      481 accaaaccgt ttagcggctt catcaatact atcaggattt tttaccgcag cactattcat
      541 tgatattttg gatgcgccag cttccaatac cagttcaata tcttcaatgc ttctaattcc
      601 accaccaaca gtcaaaggca tggtaataac accggaaaca ttttttaccc attccagacg
      661 agttttgcga ttttccactg tcgcagcaat atcaagcatg gcaagctcat cggcaccttc
      721 atgttgataa tacactgcat tctcaaccgg gtcgcctgca tctttgatgt ttatgaaatt
      781 aacacccttc acaactctcc cttctttcat gtcaaggcag ggcataatgg taatcgtttt
      841 cataggtgcc atccttttat tttgcatact atacaacttc tcatatcaca tgtatgaagt
      901 atcatacaat tcagtcaaga tataaactgt tataggctac ctttttgaat ccagtttgtt
      961 accatagtaa ctatcgcact aaaagtttgt gtattgttat tttgattggc aatagcatca
     1021 gcatcaccaa ctatcaatag tttgctgtca gcatacatat atccagcatc agtataatca
     1081 ctggattgat acatcgggat accatcgttt ataacctcca aaccgggatg tgccacctcg
     1141 ccccagaacg ccgacggaat tttgaaaagc atgccatcgc ttcggtggga tagcaaagcg
     1201 gccaggctgt taaacactaa ggaattttta tcatgggcga tagttaccat ataatagtta
     1261 ctatcgccat tagtaataaa cgccggcaaa atttgtattt ttgcagactt tgcaaacgat
     1321 acatacaccg aaggatttac tgctccagcc tttaacagtt gatttcccca taccgttgaa
     1381 gacatgagat tttgatatgg ttcaataaat acaatggtac gcttaccttc tttaacagta
     1441 aactgtggct gtggatgcac aacaattgtt atattactat cagtggtaat tgaaggaatt
     1501 ttttccttaa gtgcatccat aaaacattgt atcccggaac atgaattatc aatctgaacc
     1561 attgaatttt ccgggtcaaa acaacggaca tttgtggtgt gatgcacaat cttattgagc
     1621 ctttttttaa gaagccactc cacacaactt ttaaaaaatg ctctgttatc acctgcattg
     1681 ataccaacct taccatcgcc attccagaac atctcgctgt caccaaccac cagcattctg
     1741 cctttaccat atatgcagcc cgcagcgatg accttatcag ttgtgccatc gcacaataaa
     1801 atttcaaacg aactattcgt tttattcatc ctggataatg aagcagcaag catatgcaaa
     1861 acattcttta atgtaaatac tttagagtat gcactattat tgagattatc aattgactgt
     1921 aatggtgtgg aaccgtttcc cgctgcatca ttattgattt gtatgcccag ttttgtagtt
     1981 acagcattta aaaattgtgc actgttatac atattctcat gctcagcaat gacaaaaagc
     2041 aatccacctt ttttaacaaa attacatagt gcatctacct caccactatc aaactgctga
     2101 tactttgcaa ctgaaagtat taatagcgtg ttggtatatc ctgacctgta acactgttgt
     2161 atacattcat ccagatgttt tatggtaata gacggtggga tgccatagtt ctttaaaaat
     2221 tgtgcaatag ttgaatagcc gcaatacccg tgatcaaaaa aacgccatga gttgttatgt
     2281 gattcatcat agatacattg cgctgtattg ggtatgtagt cctgatactg tttttgagaa
     2341 cagctcataa aacatagcaa aataataaaa actattcttt tcatactatc attccattga
     2401 aaaatatgtc caacaactca taataaaaaa tccagaatgc aaatgtcaaa acaaaaagtt
     2461 tttcaggcgc tttgtaatgt gtctccactt tcaatcatta ctgggttcat aaaaaaacga
     2521 ggctgtttta aaacagcctc gctataccat ccactgattt ttataacttt cattatttca
     2581 caacttttgg caaatcatgt aatgctttgt caattaattc tctgggataa tcataatctt
     2641 ctaactgtcc tgataaaaac ttatcatatg atgctaaatc aaaatggcca tgaccactta
     2701 gattaaatac aatagtcttc tttttgcctt cttcctttgc tttctgcgct tcaataatgg
     2761 ctgcccgtat agcatgggat gactccggag caggaataat accttcagtc tttgagaata
     2821 aaattgcagc ttcaaaacat tcattttgat gcagtgccat ggcgctgatt aatccctcat
     2881 gatacaagta gctaaccaga ggcgacatac catgataacg caatccgcca gcatggattg
     2941 atggcggtat aaaggaatgc cctaatgtat acatgggcat aattggcgtc aatttagcaa
     3001 catccccaaa atcatacgta taaattcctt ttgttaatgt tggacatgcc gacggttcaa
     3061 ccgctatgat atttatatcc ttcccattga tcttatccct tacaaatggg aatgccaacc
     3121 ctgaaaaatt agaacctcca ccaacacaac ctataactac atcaggataa tcatctacca
     3181 tttcaaactg ttttaatgtt tccagaccta taatggtttg atgcaggcag acatgattta
     3241 acacgctccc cagtgaataa ttggtatcct tgctctgtgc agcttcttct actgcctcgc
     3301 ttattgcaat gccaaggctt ccagggcttt caggatcctg ctttaaaatt tccctgccgc
     3361 tattggtaat attactggga cttggggtaa ccttggcacc ccatgtttcc atcataaatt
     3421 tacgatatgg tttttgctca tagcttaccc gtaccatata aacatgtaca tcaataccaa
     3481 aaaaattacc ggcaagtgcc agagcactac cccattgccc tgctccggtt tcggttgata
     3541 gttttttaac accttctttt ttattaaaat atgcttgagc cacagctgta tttaacttat
     3601 ggctgccagc cggagaaaca cctccgtgtt tataatagat agttgccggg gtgcccaatg
     3661 ccgcctccag ccttcgtgct ctaaaaagtg gtgaaggtcg ccatagtcgg taaatgtgca
     3721 atatctcttc aggtatatca atatattgtt ttgagcttac ttcctgttca atcagtccca
     3781 tggggaaaat cgcatgcaat tcatcaggtg ttaccggctt ccctgttgca ggacttatgg
     3841 gtgggtctaa cggttttggc agatctgctg tgatattata ccattgacgg ggcatctccc
     3901 tttcaggtaa ttgtattttg gttgaatctt ttgccatgaa atcctcctta cagtaaattt
     3961 aaataatcag atttaaaata tgcatttttt aaaaaaataa aaaagctacg gttaacatat
     4021 agcatccgta gccttcaagg atttgcatat gtaaaaaact tccttactcg cccggatata
     4081 tgtaatcctg gcgccaccac cataccacat aaaacacgat gttacatttt ttaatcatca
     4141 tatataatac aatttcaaca aatgacttga tagcattaga taaaacaatt atgtaatgtc
     4201 aagaaatttt ttttttaaaa tttactcatg caaatacatt ttttgtgtga gcaagcaaaa
     4261 gttcatatat gccatcaaaa ccaccattac tcattaccac catgaccgtg cgtttattgc
     4321 tcaatccact aaatgcttcc tgcactattt cgttaacact ccctaaaaca caggctggta
     4381 tttgcatctg gcatagtttg ttaatgaccc tatcaatatc aatctgttcg tgcgccggca
     4441 ccttatccaa tctatagggt cgtttcataa ttaccttatg tgcttttgaa aaagctaaag
     4501 gaagctcatc ttgaaataca ttccgccggc tggttgcgga gcgaggctca tagattgcaa
     4561 caatggcagc atcgctgtaa tgctctgaca cctgtgtcaa aacattgctg atagcggtag
     4621 gatgatgggc aaaatcctct attattgtta gatagtcatt tgaaaagagt atctcctgcc
     4681 tgcgttttac tccctgaaat gaattgattg ccataactat cgccccaaca ggaacttgaa
     4741 gggtcagagc catagcaacc gccgcagtta tattctggtg attaaagtcg ccatatatgt
     4801 gtggcgttac attgattgcc atgtcatcca ttgtaatggt aagaacttca ttttgatacc
     4861 gataacaaca atctgcgctc attttcccat aactaaccgt tgaggatagc gcatcattat
     4921 ttattgtgct taaattacta taggcgttat tataaacaat atatccattt gcaggaatga
     4981 gattgcgcaa ccgtgtaaac catactgcaa tctcatcaat attgttatat atatcagcat
     5041 gatcaaactc aagcgatgtc atgatgcagt gcaccggacg atacataata aattttggca
     5101 tcttttcaaa aaatgccgaa tcatattcat cagcctctat aacaaaatac ctgccgctgc
     5161 caaccttata atttgtttca aaatttttag ggatgccgcc aataaaaaat gatggattca
     5221 tatcagcctg ttcaagtata tatgccagta acgatgttgt ggttgttttc ccgtgtgttc
     5281 ctgcaactcc aatgacttct ttatcataaa gaaaaaaatt atataatgca ccagccatcg
     5341 agaaataagg tatccggtta ttgagcacca gttcaacctc aggattaccg cggctaatag
     5401 cattacctat aataacgata tcaggtttaa tttttcccgg agtatcagag ctgtatcctt
     5461 tgcataccgg tatctgtaat ttttcaagct gtgtactaat tggtggataa acatcagtgt
     5521 ccgagccagt aaccatgtgt ccctgtgact gcagcatacc ggcaagcgca ctcatcccca
     5581 ctccacaaat gcctatcata tggatgcgta aattttgttt tttaaagata gtaaccattg
     5641 attaacatcc tcttttataa ataatttaca taacaaattg ctaccatata aaccacttat
     5701 tataagtaca acacaatcat tagtaaacat cgttgcttgt aaagcttgtt ttacaggtgc
     5761 aaaacattta tagttgccaa aatgtaagaa tattgttgac aatgctgcag taacgtatag
     5821 tatttcaatc cactatatag tagtgagggc ataatgaaat ccaatataaa aagcaaagat
     5881 tatcaaaact ttttagatga tctgaaaggt tattatcagg aaaacgagca aaaggaagaa
     5941 gaaaatatac cggttggcac acggattagt caaatacgcc agatgcaggg tgttgatagt
     6001 gcaacgttag caaaaaatgc cggtattgat cataaatatt tagaggatgt tgaatcatta
     6061 gcaatttatc ccgatttagg aacaattatt aaaataacca atgcgctcaa agtttcaacc
     6121 ggctcaattt ttgacttaga ctcaagtcgt tcatacacca taatcagaaa aatcgataga
     6181 aaaaaaatta tgagaagtat atccggagta aaagaacacc ctgattatga atatctttca
     6241 ttgtcctccg gcgttatatc ccgccatatg gaagctttta ttgtcacctt gaaaagtaat
     6301 gtttattata cagaggaatt atcaacacat gaaggcgagg aatttctata tatgctggaa
     6361 ggagcaatga agataaaatt agacgataaa gaagaaacat tgcagcaggg cgacagcatt
     6421 ttttaccact caaccattcc tcatgtgctg aaaagcatca gcccaaaacc tgcaatactt
     6481 cttgcagtcc tctatacggg ctattcttca taactttagt atacagcaaa ttgtaatatg
     6541 gcagtgtaat tattttatca gtgcaaaact aataagcagt aactatcgcc cagcattgtt
     6601 tattgtttta gcacccatct gtatttaaca atgttaaaca ccttctgaat agagttaacg
     6661 ataaacagga atgcatcacg gatatcaaac attgggctgg gcaacccaaa cagcaatggc
     6721 acaggtataa aaagatttcg tgcgccaata ggacatcctt taatccgcat tacccgtttt
     6781 gccacaagct tgccatcaac ttttgtgcaa tctcccacaa gcaaaacatt gccatcgggc
     6841 acaacaatat cacctttgta aataccggta acaattgcgc ccttctttgc tttctttaat
     6901 gaaccaggcc ttcgtttttc aatagttccc aaacaacctt taatagctgc ttcgcaaccg
     6961 ccatcacaga taatgtcagt attgggtgca taccctgaat aaaatgtaag aggcgtgtca
     7021 agctcttgca gtacctgaaa cagtttacgc tgaccttttg gttttgctct tagtttatca
     7081 atactgatat ctccggtaat attaatgtct tttaattgca atgagccata tccgcgttca
     7141 tgtgcaagct gtaaatgcaa aacatcctgc gggcgatagt tcataatata cgatgcaacc
     7201 acatcacaag ccagtggatt attggatagt atttaagctt tggcatccat attttgaagt
     7261 tagcttctgc aatatatttt gataattcta tctctttgtg ccatttagct ttagaaagtt
     7321 ttactttaac aacgggatgc tcattcaaat ctacaacagg aacattaagc ttttcagcaa
     7381 gatcaaaata tcctgcttct ttaaggaata gtcgggaagg tatgccatat ccacctgatt
     7441 cgccaatgat aatattattt atgcttttat cttttagcac agtaaccata gcttcaacca
     7501 cagccggatg ggtatacgag ttgaagatat attgtttatt tgctgttacc acatttggtt
     7561 taataaaaac gttttcctga atggggattt ttaattttaa atcattcacc gactcattta
     7621 taatagcaga aattgccgac atatcataat catcacatct tcgtacaata acatgcggtt
     7681 ttttcatata attagctcct taaaaatttt tcaccagcaa aacctgattg cccttattat
     7741 taaatttaac ctcatcaaaa atattgtata ccatgcgcaa acctcttcca tgtgccagca
     7801 tatcatcatc aacagtattc tgaatttttt gaataatatc tctataatta aatccattac
     7861 cttcatcttc tattttatac ataaccttgt tgcaattgag taataattct atagtaactt
     7921 ttttgtcttt ataattggga tctttttgcc tgcttaaaac aaattcaaaa taattgccat
     7981 tattggttgc ctgcgttttt tcttcaaagc caatatttaa atttccatgt tctatggcat
     8041 tgatgattat ttcacgtaaa cctaatttaa tagtatttgt agttttttta tcaagatatt
     8101 ttggtaaatt caaaaccaac cggttacata tctcttcagc agcaatcaga taatttccaa
     8161 tggatagttt tagcttttca gcttcaatat attttaatat attctcatcc agaactgatg
     8221 ttgccttcat caatatttca tttcggtcat caatcactat atgctcaaag cggatagtat
     8281 attcttttgg ctctaaagtg ccgcgtgact ttagctgtat ttttaaattg atgggatttc
     8341 cctcggtaaa aagcatgttt atcttttctt taattatctg caatgtcagg tcgctttcac
     8401 cttccggcaa gtaaaccaga tcataaaatg atgttgcaga ctccagccct ctcctgacgg
     8461 caagctgttc atatgctgca cggtttgctg tagtaatatt taaattagaa tcaaatgata
     8521 atatacagtc attggtacct tcaacaagaa atctgtattt cttttcggat atgcttatta
     8581 ttttatgagc tatctccaat tcctgtgcat agtttatgaa catacttgta acagatgaca
     8641 tgaaaaacat tattgcaagt gtaataccaa cactgaaccg tgaaaaatag acattgggga
     8701 agaggtcata gaatacctgt gtatgtacat gtaaggtatc aatggcagca ccagcaaatg
     8761 caaacaacaa acctaacgca ggatataaca aatagcgata gttcctgtgc aaaatcatat
     8821 ctatcatcac agcaacgata ctgtaaatta tatagatacc cagaagaaca tcacgaatag
     8881 catacagtat gccttcctgt cctcgtccat aatcagcttc atagtacatc catgtagctt
     8941 tatgttttgt tagcgatata aaaaggtcag ggaatacaaa tgcaccgata gtagctataa
     9001 cagcataagc taatccaata tatgctaaaa tagtatttat tttaaaaaac cgcttattta
     9061 aaatcagtaa tttgtttaat gaataaggaa gagcaaaaag ataaaaaagc cctgctgtct
     9121 gttccaatcg gtgaaaatga acagatactg agcgattatg cagccatcca ccaaatgaca
     9181 gtatcattgt ctcgctacca acaaaaagca tccctaacag tgcaaaaaaa gcaatagcaa
     9241 aatataaaga atttttatac ttccagtaca tatagacata taatataaat cccccaataa
     9301 ggagtccaaa acacattggc ggtaataaaa actgaaccag gcttacaggc acttcaaaac
     9361 tcatgctctt ccccactata ataaaactaa actattttac ctcaaagcga atcatgttta
     9421 ctacattgct ggttgacaaa atcacatcaa agtatttatc gggttctaaa taggtaatac
     9481 gtgtacggcc atcactaaga gcaacctctt cctgtggttc ccccataatt tttattacct
     9541 catctttgct cataccaata tcaatttttc tgatttttcc acctttccat tccggataaa
     9601 acaatatctg tcgtatcatt tcatggtgga atactaaacc aaactgtaaa taatcaaaga
     9661 tgatataatc ttcttctttt gttggtcctc tgtcaacata cattttaacg ggaactccat
     9721 atttacaaaa tacctttgaa tgacgtagtc ctaacacatc gatgagctcc tctgccgaag
     9781 gtgttttgtc agccgcgatg aaaacaaaaa caactacaac tacagcaaca acccctacta
     9841 tacgcttcat atacagcctc ctttgaaata atattgtacc attaatgata ataatgataa
     9901 aaacgtactc acaggggttg gaaaattgca agttattaat ttttttatat ctttttcagg
     9961 tcgcttttaa aataatttta cctatggtat acaatagttt tcagcagcat atgtatataa
    10021 ttttttgatg tgatgaatgt aatcacccgt ttattgattg gatatactaa tcatcagtac
    10081 ccatggtggg atggtacaat acccttaaag aactttgttc attagtcatc agttaaaaaa
    10141 acctattaat acaataacca tatatcagcc aataataaaa ttagacttga ctaattatat
    10201 tatattcatt aaattaacac tatatttttg attaataata actatcgcac ataatatgaa
    10261 gatgagaaaa agaaaatcca gcccatgggt aaacaatcca accaatgagt tgaatgatac
    10321 cggtcttact gcaaatatgg aagactatat ggaaaatatt gctctgcttt taaacgaaaa
    10381 tgatgttgtt cgtgtaaaag atattgctca aaagctcaac atcaccatgc caagtgttac
    10441 tgctgcattg caaaaactaa aggaaaaagg actggttgat tacgaaaaat atggccatgt
    10501 acagcttacc ccccgtggta aagagattgc cgattcagtt taccataagc actcattttt
    10561 aagcgaattt tttcaatcaa tcctgaacat ggataaaaaa agtgccgagg agcaggcatg
    10621 caagctggag caccatttaa gtaacgatgc cagtacacgg atgcaacagt ttgttgaatt
    10681 ttataaacat gaacagcagg ctaatgaaca atggataaaa agactggaca acacattaga
    10741 ggaaaggaga ttaagccagc taaaagaagg gcaaaaggct gaaatagttg caatattggg
    10801 aagcggtcca ttaaagaaac gattgcttga gatgggattt agaaagggtg aaattataca
    10861 tataataaaa tatgcaccgc tccatgatcc tatagaaatt gaaataaagg atttcttaat
    10921 ttctatccgt gttgaggaag ctaattatat cattgttaaa ccaattgacg agtagcccac
    10981 catgacaaaa aaaacgataa cagtaggact gataggcaat ccaaattgcg gaaaaacaac
    11041 catttttaat gccttaaccg gtgcacgtca aaaagtagct aactgggctg gtgttaccgt
    11101 agaacgccgt gaaggttttt ttaaccataa agggtatacc atcaaggttg ttgatttacc
    11161 cggtgcctat agtcttacat cgttcagcat ggaagagctt attacccgca attttatcca
    11221 gaatgataag cctgacttca ttatcaatgt tattgacgca ggcaatgtag aacgcaacct
    11281 ttatttaaca acgcaactga tagatattga agcgccggtt atcattgtac tcaatatgat
    11341 tgatgaagct tttgataaag gactcatcat aaatatacaa aaattaagtc aattgttcaa
    11401 tatgcctgtt ataccaacgg ttggaacaaa aggaactggt atcccacaac ttaaagatag
    11461 cattgtagaa aattttgaaa aacaaacaaa aaatcataca ataactatcc cctatggcaa
    11521 tgatattgaa caggctattc aacaattaca acagcttatt gataaaaaca gattagaata
    11581 tccttcacga tggctggcaa taaacctcat agagggcgac atcgatgctg aaaaaagagt
    11641 gaaattgttg ccagacggca atgaaataat acagaaggct tataccatcc gcaacaatct
    11701 ttcgaaagct tttcatgatg atatccagac agtttttgcc gacatgcgtt acggcttcat
    11761 caccggtgca ttaaaagagg cagtacaaaa aaagccaatc aaacgtcatg acctgacaaa
    11821 aaaaattgac tcggttgttc ttaatcgatt tttaggattt ccattacttt ttgtcatcct
    11881 gtttatcctt ttcaatctta catttatact gggcgaatac cccatgaaac ttattgaagc
    11941 gggtgtagaa tggttaagtc attatttgat ggaaattatc cctcctggcg aattacaaaa
    12001 ttgtattgtt caaggaattg ttggtggtgt aggaagtgtt attgtttttt tgcctaatat
    12061 tctgatcctt tttttaggaa ttagcttcat ggaagatacc gggtatatgg cacgctccgc
    12121 atttttaatg gacaaaataa tgcacagcat gggcctgcat ggcaaatcgt ttattccgct
    12181 ggttatgggt tttggatgta atgtcccagc aataatggca acccgcacac tggaaaataa
    12241 gcgcgatcgc atactaacgg tgcttatcaa tccatttatg agctgctctg cgcgattacc
    12301 tgtatatatt ttgtttgccg gtactttttt tcctaatcat gcaggtttta tcatcctgtt
    12361 tatttatgca ttgggcatta ttctggcatt tacttcggca aagctattca acaacactat
    12421 tctgaaaggc aaatcaacac cgtttgttat ggaacttccg ccatatcgca tgccaaccac
    12481 taaaacaatt gttatacaca tgtgggacag agcatcaagc tatttacgaa aaatgggtgg
    12541 aataatttta gctttttcaa ttattatatg ggtattgagc gaatatccaa aaccttacca
    12601 tatccaaaaa caatttgaca caatgataca gcaaaaaata attgagttga atcaaacatt
    12661 gagtacttat caaaaacaaa atgctgaccc aaacacaatc cagacaattc ataaacagta
    12721 taacacaata attgaagatt tagagataca aaagcgccag gaaatggtac agtatacctt
    12781 tatcggcagg attggttctt ttatctatcc tctgttacaa ccgttaggat ttaactggca
    12841 gatggggata tcgcttacca caggttttgt agcaaaagaa attgtagtaa gcacaatggg
    12901 tgtattgtat catgctgcta atgatgaaaa tactgtcagc ttatcacaaa agcttggcaa
    12961 tccccgttat ggtatcactc ctcacgcagc attagcattc atgatatttg tcatgatata
    13021 tattccctgt ttagccacag tgattgctat tgctcgcgag attggtaccc gctgggcttt
    13081 tttttcagtt gcatatcaga ttacagttgc atggatagta agttttattg tttatcaaat
    13141 tgcacgtttt attctttagg ttctggcgat agttactgtt taaaactacc ttcacccctc
    13201 ctgaaactat cctgaatagc ttttatggtg ctactatatc ccttgcgcaa tttatttccc
    13261 atatacaacc attgaagccg gagatgataa ggtttttgct gatacccatt taaagtagca
    13321 tcatcaatgt aataacgaaa tcctttattt gtattatcgt tcatctttta tcctttttaa
    13381 atattctata tcactatatc ctgatctcgc attgacacct tcttcattgt aataagatca
    13441 tcaatagata taatgggcaa ctttatgcca tttattgtga caaactttgc tctggttatt
    13501 gcctcttcat aattaatggg agaatcaata ataatatcaa tcttggaaat tgcccaatca
    13561 ggattagcaa agcaaaatgc tttcatactt ttatttttta tnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13681 nnnnnnnnnn nnnnnnnnnn ngaagatttc aaacgtatag tttcatcaat gaactgaata
    13741 ctacaactca accaaaaata gaaatataca ccagccggtt attcctggct tgacccggaa
    13801 tccctatata taacgttaaa tcttgaatca gcgttcagga tgacactaca ctatggtgta
    13861 taaaatacca actggactga atttttttct tgcaattttt tatgatatat tgcaattttg
    13921 catagtgttc acattattat aatgtcaaat attagttgtt aattaatgaa tagtgatatc
    13981 aactttacag aaaatgctga tcatgtaaaa tatttactac agattgcaaa tcttgatact
    14041 acgtcttccc tatcagagta tgttcacaat ctaaaaacaa ctatcgccca gctaaaaatt
    14101 cctgttttta atatttcctg cctttcaaac atcgaaggca ccgatcatct attaatattt
    14161 cccgattggg attaccttaa gcaatgggtg cctgagctat ccatatcaaa caacatcttt
    14221 gaatatgcca ccatcaacat taaaaagtta attgccaatc gtgctatttt taaaaaaagt
    14281 atattgcatt cactattacg cggttattta acactgaccg atactatttc catacctgag
    14341 gtaatacagt atcataaagc aattgtttca tgtgaacaaa tgataagcga tttttctcaa
    14401 gcaagtaagg aattatttca gatactcaat cctgatgttc agaatcgtat tctggagtca
    14461 cctctttttg gcagcgctat atcatttcca cttttttccg atagagatgt cataggctcg
    14521 tttgatgttg tttatccaaa aaactatcct gaaataaatg acaatgaatt tgcaaccctt
    14581 gaacttattg caaaattaat agccagtggg ttgcggcaat atcgttacca gaataaaatt
    14641 attatttctg aaacaaaata ttcttcgctt gtcaatgcta tccctatggc attatttgtc
    14701 attaacaaag agtattctat tatagaaact aacgatacct ttaaaaaatg gtttaacata
    14761 gagcgcccac tcaataaaaa atgttatgag atagttggtt gtagcaataa aacctgtaat
    14821 ggctgccctg cctcaaaagc aatggacaaa gcagctccca tactacagga aaaaaaagtt
    14881 ccttatttta aggatacacg tttttttaac tatattactt cccccatcat tgcaccatcg
    14941 ggcagcatct cttcagtagt tgttattatg gaggacataa caacacgtat caatgccgag
    15001 aaaaaaatag aaaaatttag ccttacatta gcagaagaag tagacaatca aacaaaacaa
    15061 ctaaaggaaa aacagcaaaa attgcagtta accaccaaca cgttgtatgc attgaaacag
    15121 gctaacacca taaaagaaag tatacactgt attattgaat cactgcatac attgggttgt
    15181 aaatattgta ttgtaacttt actcagcagt gatgtcaata cacttcaaat agttgaagta
    15241 tatcctgcca gctttttgca attgcttcaa gcggttacaa atcaaaatat tactactata
    15301 aaatttgatt ttgatgattc agcaaatata ccgttttttt cggttattaa aagcggtaaa
    15361 gaaattatga tagaacattt ttctgaattg atatcattcc ttgaatcaac atttccacaa
    15421 attgaaagcg ataagataaa acaaattagt tcattactaa aagatgaagt aattggcatt
    15481 tatcctcttg ggtcaaaaca ccagatagaa ggaagtatag caattgcttt tgacaaacag
    15541 tcaattgaag aaaacaagga ttatatagag atattactca atgctgcagc ggttgaaata
    15601 aaccgtaagc gaaatcagga agagcttgta aaatcggaat taaaatatcg taatctggta
    15661 gaaaatactc aggatataat aatgctgtgc actgcagaag gtgaaatcat ttacggcaac
    15721 tcggcttttt ataaactgct ggcatatccc gaaaccagaa aaccttcatt ttttaatttc
    15781 attgatgata atgaaaaata tttaatacaa tcaataatgc aacatttgct taattatgga
    15841 attaatcctg acccatttga gattcagatg tatacctgtg atgaaaaatt tttatggttt
    15901 gaaattgcca tgagcatgat accacttgat catacggtag gtattcagat tgttgctcgt
    15961 gatattgaac gtaaaaaaag aatggaatca catatacaaa acctaacaga atttcaggaa
    16021 aagatattac aaaatgagat gattggaatt ataactactg atctgagcgg tatcattacc
    16081 agctggaata aggcagcaac aagtatactg caatatcagc cctttgaaac actcaatact
    16141 cccttgcaaa atcttattct taattattat gaagattcag ttttgtcaaa atcaaatact
    16201 tttgcctcaa tcataaacca accgtataca gagattaaga tgcttaaaaa aggtggcact
    16261 attattacca ccctgtgcat gtcatctctt ttacatgatg atcatgacat gccatttgca
    16321 cgtcttcact tttttattga catcagtgag aagaagcaac ttgaagctga atcattagag
    16381 ctaaataaaa aactccagca tgcccagctg gttaccatag tatcactggc aaagttggca
    16441 gaatatcgta acaaagaaac tggcatgcat cttgaacgta tcatgcgcta tgttgaaatt
    16501 ttagcacggg agctatcaca acatcctgat tatagagact atataaccga agaatatata
    16561 caggatctgg taaattcatg tcttttacat gatattggga aggttggagt tcctgatcat
    16621 attttgctta aacctggaaa acttaccgac tatgaatatg agataatgaa aacacataca
    16681 ataataggtg ctgaaactat agcggaagca gaaaaaaagg ttgaaggacg ttcctatctc
    16741 aatttaggga aagaaattgc ctattatcat catgaacgat gggatggctc aggatacccc
    16801 aaaggtttga aaggtgatgc tatacctttt tcagcacgca tagttgctat agctgatgta
    16861 tatgatgctc ttgtttcaga acgcccctat aaacgtgctt ttcctcatga gaaggcagtt
    16921 gaaattattt ctaaaaaagc gggaacatat tttgatcctg taattgtgga agcttttata
    16981 aaacatcaca atagattttt gcagatcaaa gagacccttc tctaaacatt gtatattctt
    17041 ttatatcctt tgtatccttt atatcctttt taatacagct tttaatgaat caccactttc
    17101 tataattcca taaaaaccac tttgtgccgc tccgggatta aacatcattg gactcccatt
    17161 agtattgcaa ggcttatgag tatggccaaa acacgctata tcacatcccg aatgtttagc
    17221 ttcaatccaa actcctgtaa tgtcatgatg ggctccatat acatccccgt gggttataaa
    17281 aaatttaaaa ttgcctattg tttcaacaat tgtgcttttt tcattgatgc cccttgccct
    17341 atcgatgttg ccgcatactg ttatcacaaa cacaccttcc ggtacactca catacatcag
    17401 gtcattgatg ccatcaccgc aatgtaatag ataatcaaac ttcctttcag cttctattat
    17461 agtttttatt gcatcaatat ttccatgagt atccgaaatt accagtactt tcataaatta
    17521 cactcaataa aaagtagttg cattatttaa atcggattgg cgatagttca tcaagaataa
    17581 attttatata tttatataaa cagtctgctt tttttataac acacgctata gtttattgta
    17641 tcaataacac ggtaggtttt gattatatgg aacaatcact tatcagaaat ttttccatca
    17701 ttgcacatat tgatcatggc aaatcaacat tggctgatcg tattattgag cgttgtaaag
    17761 ctatcgaccc acgcaatcac gttgatcagc ttctggatga tatggatatt gagcgtgaac
    17821 gtggaattac cataaaagcc aacgtcatta cactgaacta tcagtcgaaa gatggaaaaa
    17881 tatatacatt taatctgata gacactcctg gtcatgttga ttttgcctat gaggtatcca
    17941 gagcattagc cgcatgtgaa ggagtattgc tgcttgtaga tgccactcag ggagttgaag
    18001 ctcaaacaat tgctaatatg tacttagctc tagaaaatga ccttaccatc ctgccggtca
    18061 ttaataaaat agacatgcca aatgctgaca tccagcgtac taaagaaagc attaaaaaaa
    18121 gtttaggact tgatcctgat acagtattat gtgtttcagc acgcgaagga attggtatag
    18181 atgaacttct ggaaaaaatc atcgaaataa tccctgcacc aaaaggcaac ccttctgcac
    18241 cacttaaggc tttaattttt gactcctatt ttgatcaata tgttggtgtt gttaccaaag
    18301 tacgaatatt tgatgggcag gttcaagaag atgatgaaat tatgctattt tcaacacaaa
    18361 agcagtatga ggttaccgag gtaggacatt ttcggctaaa gcgagaaaaa cgagtacagc
    18421 taaccgccgg tgaagtagga tacataacag cagggataaa acagctaacc gatacccgta
    18481 ccggtgatac tataacatcc gcagaaaatc ctgtttcaca cccaatcata ggatttaaag
    18541 aagcaaaacc aatggtattt tccggactgt atccatcaat aagcgatgac tatgatgatc
    18601 taaaagatgc attgtacaaa ttaaagctta atgatgcagc attacacttt gaacctgata
    18661 actcgtatgc gttaggcttt ggtttcaggt gtggttacct tggtttgctt cacatggaaa
    18721 tcgttcagga acgcctggaa cgcgaattta atttagccct tattacaact gctcctaccg
    18781 tagaataccg cgttataaaa acaacaggcg aagttctatt aattgataat ccagttaaga
    18841 tgcccgaccc atcacaaata gaacgtatag aagaaccgtt tgtacaggct actatcatta
    18901 ctccatctga atttattggc aatatcatgg ctctggttaa tgaatgccgc ggaatacaca
    18961 aaaaaatgga atatattgat ccaactagag tagaactgtt ttacgaaatg cctcttgccg
    19021 agattatgtt caatttttac gataagctaa aatcgtgcac tcgtggatat gcttcatttg
    19081 attatgaact tatcgattat agagcttcag atattgttaa ggttgatatt ctggtaaatg
    19141 aagaatcagt tgatgctctt tcattcatgg ttcatggaga taaagcatat aagcgaggcc
    19201 gtgagattat tgaaaggttg aaagaggtta tcccgcgaca acaatttaaa ataccattac
    19261 aggcagcgat aggctctcgc attattgccc gagaaaatat atcatcatta agaaagaatg
    19321 ttacagcaaa atgctatggg ggagacatta cccgtaaacg aaaacttctt gaaaaacaga
    19381 aagaaggcaa aaaacgcctt aaacaaattg gttcggttga aatacctcaa gaagcttttt
    19441 taactatctt aaagatagaa taataaaaga ataaacccta ttacgcccag gtaataagtc
    19501 gcgattcata ttttcttggc atatctgaat gtaagggaat aacccttacg gtaaatccaa
    19561 attgtccgga tttaaggcag agcatccttc cctcatagat atattttcca ccacccgatt
    19621 cttctaccat atgcataggc aatgcagccc catcctcaat ttcgcctttc tgatttaagt
    19681 ttccaaaata taattctacc tgaacatcag ttggtttcag atcacccaaa ttgactacag
    19741 cttttgcttt cagttgagaa ccaacaataa tctctttttt actttcatag gtaatagatt
    19801 caagagtaac ctgatgccat ttttgcatta tatctttttt ccacttagcc agtgaacgag
    19861 atgctttaaa atcatcctta ctgaacttca caaagttatc atgtgctgta agatagtaca
    19921 tttcagtata ctctttcacc attctatttg tgttaaattg cggcaatatt gttttcattg
    19981 attctttaat acgatttgcc cattcacgag ggataccatc tgcgcccctg ttgtaaaaaa
    20041 gtggaataac ttcttgttcc agaatattgt ataatgataa gctttcaacc tcatcttgat
    20101 aatcagggtc atggtattct tcaccctttc caattgccca tccattatta ccgttgaatg
    20161 cttctggcca ccagccatcc aatacactta tattgagtgc accattagga acaaccttca
    20221 tgccgcttgt tcccgatgcc tcctgaggcc gtattggtgt attgagccat acatcaacgc
    20281 cctgtacaat atatcgagca atattgatat cataatcttc aagaaaaaca attttgctcc
    20341 tgaaccgatc atccctgcct aaatggataa tccgcttaat taattcttta cccatattat
    20401 cccgaggatg tgccttccct gaaaatataa tctgtactgg tttttcctta tttaatagta
    20461 ttctttccaa acgttgcata tcttttagta taagtgtagc ccttttatag gtcgcaaatc
    20521 gccgtgcaaa tcctattgtc aatgcctcgg agtcaagtac tgaatctgct ttctcaatct
    20581 ctgcccttgg tgcacctcgt gcaatcaatt gttcccgtaa ctttttacgt gcaaatgcca
    20641 ctacccgttc acgcaaacgc tctctacagc gccatagctc agaatcagga atggtgtcaa
    20701 tacctttcca tattgtttta tctaccggat tatcaatcca tctaggacca aggtatctgt
    20761 tcaataacct catcatttca tcagatgtcc atgaaagaat ctggacgcca ttggtaacat
    20821 gataaattgg cacctcatta accggcaccc ctggccatat atttttccac attctacgtg
    20881 ataccgaacc atgcaattta gataccccgt tgcaatgagc agcagtttta attgctaaaa
    20941 ctgtcataca aaatgtttct attttatcat gaggattttc tctccctaat gccaaaaact
    21001 catcacgaga tatgcttaat ttttgaaaat aatgcgatag atatttatca ataagttctg
    21061 gaacaaatct atcattacct gcaggaacag gggtatgtgt ggtaaaaaca gtgctggcat
    21121 taacatattc aatggcttca taaaagttta atttatgtgt atgcatagta tcatgaatcc
    21181 gctcaatgct taaaaaagct gaatgtcctt cattcatatg ataaacggat atatttttcc
    21241 ccaatgcttt taataatctg tatccaccta tccccaacaa catttcctgt ttaatacgca
    21301 tttcaacatc accaccataa agctggtcag ttatagtacg gtcttccaga ttattttgcg
    21361 ttatatttgt atctaaaagg taaagtttaa cacggccaat attacattcc catatctgtg
    21421 caaatacaat cctgccggga tattcaactt caactaccaa aggcttatcg tcttttaaaa
    21481 tcagttgtaa tggcaaattg tgaaagtcgg tctcaggata tctttcctgc tgccagccgt
    21541 cgatattgag atactggtgg aaatatccca gacgatacaa tagcccaaca gcataaaacg
    21601 gaagtcctaa ttcacttgcc gacttgatat gatcaccagc taaaactccc aaaccgcctg
    21661 aataaattgg caaacattca tgaattccaa attcagcact aaaataagca atctgcatat
    21721 ttttataggt aggatatgca tcttcaaccc atgatttttt tgttaaatgc cactcaagct
    21781 cttcagaaac tcgttttaaa tgtgccataa aactttctga atctgctgct tcgtccaatt
    21841 cggactgtga aagcatacca agaagagcta cgggattatg gtctaaatcc ctccagagtt
    21901 caacatttag acgtctgaaa agctctatag cttcaaaatt ccacacccac cacaaattat
    21961 atgcaattgt ttttaatggt ttaagattat caggtaaata tggatatacc ctgaatgttt
    22021 gtatagttcc catagtctcc acctataata aatagtaatc gggcaatagt agtttctcag
    22081 taaaaacaag tcaagatttt aataatgata ctgataattt tattagtttt tagaaataac
    22141 tatcacacat ttttttaaca tctcaccaaa taaatgatat attgttatga gatagttatt
    22201 attaattgat atattgactt acaaacagtt tctcattatg tatcaaagga ttgtctatgg
    22261 attttacaat cgtattgtat caatatccat taggaagttt catcacgcat cacacctttc
    22321 aaaatttaca gagaataaaa ccgcacttca tttgttttcc tgaatatttc tttgtaaaca
    22381 aaaaattagg taaccatgcc cagacttatc acaatcaaca attacaattg aagcgcatac
    22441 agctattatc acgtgagctt gacacagttg tcattggcgg tacaatgccc gaattaaatg
    22501 gaaataaatt gcacaacaca acctttgttt ttcacaatgg gacaatactg ggtttttatc
    22561 gtaaacaaaa cctttttttt gccgaagaag ggaaaattac tccgggcaat ggtcatgtta
    22621 tatttaatgc gtatggcata cgattcagtg ttcttatatg tgcagatgtt tttaaggatg
    22681 aaaattttat tgctcttaaa gagcaggggg tgcaaataat atttattcca acattttcac
    22741 tattccgtaa tgaaacaatc gacgaaaaat ataaacgtga taatgatata ttcatacggg
    22801 gagcaaaatt agctgatgca attatagtta aggtatgcgg agtaaaatcc gattataaaa
    22861 attttttaca ggcacgaagc cttattgcat cacccgaagg tattctttac cgagttgaac
    22921 ctgaacagga agataaatca atgctgatag taaatactat tactatctaa tgcttcacca
    22981 tcctcccaat ttgtttggct gttataaata atagtactcc atattttaat taataaaata
    23041 ctttggaact tacactataa tcccggttaa ttttgattta atgaactgcg gtaacgcaaa
    23101 agcccctcta tgaatatcag gattataata ttgtaattgt ggcaatagca cctgtgctct
    23161 ttcttgtata aaatcattga gcgggtgata tttctttgaa caaaatgaaa aaccaatcat
    23221 accgcttgga taagttggaa ccatggtgta atagtatgat ggcaccttaa aatatttttt
    23281 attatagttt gctaaggtca caatgatatt gctgtgataa aatattgatt cagattgagt
    23341 ggatgcaata ccatctgaat ccaaacattg gcttacattt ttataaaatt gttctgaaaa
    23401 taacactgct gctggaccta ttgggtcgga tgaatctaca agaattatat cataagcatt
    23461 ttttctttct gcaatgaatt ttgcaccatc ttcagtataa acggtaaccc ttggatcatc
    23521 aaatgaacag gataaagcag gcatatattc tttgcacaat ctgataacat caccatcaat
    23581 ttcgcatata tcagcttttt ctacttcttt atgctttaat acttctcgca atgtaccacc
    23641 atctccacca cctatcacaa gaacacgttt aggagctgga tgtacacaca tgggaacatg
    23701 ggctatcatt tcatgataac ccatctcatc aagttcggtt gtcatgaata ctccatcaag
    23761 caccaacaac ctcccaaatg cttttgtttc atatatttca atcttttgaa attttgaatc
    23821 ataggttact aatttatcaa taacctttaa tttcatagtt cttccacttt ctacttcata
    23881 tatttcatca atccaaacat ttgattcaaa aggcatgaat tacctccatt ttcaataata
    23941 gtatatgcat ccagctaagt gtaaaaatga catttaaaaa tcattgcaac tgtttggtaa
    24001 agattctttc ctaaaataga atagtttcgt aaaaaaatta cttaccaata ccttatactg
    24061 gtacagtgtc acattactat ggtttataaa catacattgc aacttccttt acaattattt
    24121 aacatatgga aaggtactga atcacttaca attattaata gtttatttta tttaaaggtt
    24181 ggtggatgga aatccatcca cctttttatc atctatgtta ctttctcaat gctacattca
    24241 atgtatagtc actacctttg aaataactta gtgaaaactg agcagctatt tcaggatcgt
    24301 aatatttaca gctaaatata tcaatataag ctgtattggt aagatttgca aagtgtcctg
    24361 atatcattga tgtttctata agctgtacca ttgaaaatcc agcaacacgc tcatcttcac
    24421 caaaattaac aacttgagtt tctccaaatc gtttcatttc aataaggtta cataactgaa
    24481 tagcatactg ccgtatcatc tccgcatcac gaataatatg cggatcacat ttatggatat
    24541 cgacactaca atataatccc catgcaccta attgttcaaa ttgattattg gtcggaattt
    24601 ttatttgaat ctcgctattc aatgtagtta acattttcta cctcccgaat tactggaata
    24661 catttaacct tactatcgtt catcacacct ctacgcatct catacagaac acagcgatct
    24721 gcttccagtt tttcagtgat ataatcgata gcatttttat agtcatgatc tccgcaggtg
    24781 aagacgtcaa cagcagcata cccatactcc ggccatgtgt ggattgtgat atgcgactcg
    24841 gctatgacga ttactccgct aataccgtaa ggagaaaact tatgaaaaaa tggttggatg
    24901 atagtggctt tagagagttc ggctgcggcc gtcaatacct cctcaacctt tttcaaattg
    24961 ttgatgtgga acgcgttgca gttgaagagc tccgcaataa tgtgtgtacc taaaccctcc
    25021 atgggcaacc acctcataat tataaaatca cctacactac ggtgagtgta tgcattaaga
    25081 atggttttta aggttaactt aaattcgcca ccctggggat tgtgttcctt tgggttcttt
    25141 gcgctttacc ttacatgata tgctaaaatc ttcttcagat tttagctaaa aataatatca
    25201 ccccctctgc tcaaacagaa cattgtcagt attatatcac cttcaaaaat ttcaacaata
    25261 atattgcaac attcaaaaaa aatttaataa aaattaataa taaagtataa ggtttaataa
    25321 agaatttgaa aaatgattga ctacaatgta tattataaat agattgtcac atcatgttaa
    25381 atgaccgcgc ggtggagcag ctggtagctc gttgggctca taacccaaag gtcgcaggtt
    25441 caaatcctgc ccgcgctaat aaggcggagt agctcagtag gttagagcac acggctcata
    25501 tccgtggtgt ggggggttcg agtccctcct ccgctaatct tctttagtgt aatttctttt
    25561 acaaacatca cataatccat gcgatgtttt tttgtttgca tattcattaa aatatttgtc
    25621 cagtgaaatc cattcaccat taataaaaat tttgtgacaa cgagcacata catagatata
    25681 tgactctaac tttcccattg aaagattaat ttttattgtt tcatataata aaatgccaaa
    25741 aagtattgtt ataacaaccg tttccattaa gcattccaca aaatttattg gcgtgggctc
    25801 ttcattaaaa ataatatatg gcaaatcaag tatctcttca aaccaaaaaa acataaccag
    25861 taacaaaaag acaatgaatt caattataac tatttttttt aatgtgtatt tatttttcat
    25921 aatactattt tacctcatca atttttgtaa ccatggttgt gacaatgata ttgccgactc
    25981 aggacatatc tccatacaac aatagcatcg tatgcatttt gaataattat aattgggaat
    26041 tgtttctcca tcttttgcaa tactgattgc tttagcaggg catatctgtt tacattgata
    26101 acacagagtg catttattat catcaggtaa tggcttagca ataatcaaat ttttcaataa
    26161 tctatttaca attgagatag atagtattga ttgtgaacct gattcttttg gaggtatgca
    26221 gttgtagtta ttttcaatag ctgcatcttt gactatggta attgcatcac tcacagcaca
    26281 tatattcctc ttcataccca ttgtaatggt aggaatttca tcaatagtaa aaccagcaat
    26341 ctttgaaagt gcataatcaa cggctatagc attatatgat gctccaatga ttcccataaa
    26401 ttttgggtta cctttaagcc ccggaccatc tccttccaga atggtaattg catcaacaat
    26461 atgtatgata gtttttggca atgaactatc agtaatatat gcttcatata aatccaacaa
    26521 catctcggaa aattcggatt ttgtttttgc cttaatatgc cattgggatt ttttcatacc
    26581 atggattgtt ccaaaaagat ttttcacagc gcatgtaata taggttaacg catgagtctt
    26641 aaatttggga agattgatga tgatatcaca ttctgtaaat atttttggaa cttcaaaata
    26701 tttaaaatgc cgtgcatttt cattatgtat gataatataa tctgaagtat cagcaacctg
    26761 aatctcctca tcagcaacta tcgcgtcata acctgctttt ttcatcacac gctgcaatga
    26821 atgtacggct ggtgactcaa caaggatggg tattccaccg tgttgcttaa taacatgaac
    26881 tatcgccctg aaaaataaag gatgcgtaac aactgctgct tcaggtgcag ttgatgttaa
    26941 aagatttggc ttgatagcaa cctttgcatt ataaaaccgt gaaaaatcaa aatgcaatgc
    27001 attcaatgca ctggtaattt gttttgtaac aatatcatcc ttataggttt tacattcttt
    27061 taaaataact tccatatttc ttccttatga atacactata cgatatacaa ctgttaacgg
    27121 acaatttcaa tatttaacgc atgtagtact ataattttct gagcaactat attggtaata
    27181 attccttcaa cattagctat tgtgccattg gttatactgt tatcagcagt agatgaaaaa
    27241 acttcaacca caccatcaaa tctgtcattt tcataattta ttaataattg aaatgtatga
    27301 ttcctgcctt gttttttata atttgctact ttaccacgcc atttaacatt tatcccctta
    27361 tatagatacg gtttctttat aacatcatta tacaagtaac ctgaaactgt cctttctcta
    27421 tttctaatga tgaaatcctt taaaaatgca acacgttgtt taaccttcat ctgaacatta
    27481 cttgcatcta ttttattcag aataatcatt gcgtcatttt ctttaccttc tttgatcagc
    27541 cacttagcat gattaaaatc acgtatacac tcttctccgg aagtataaaa ttcaggtgtt
    27601 ttaaattttt gtatggtatc tataacatca taatgcatac catcaagtgt tatgtttgat
    27661 agttcatgta tatctgaccg tgtttcagta aaaagtaacg aaattttttg ggttataagt
    27721 tttgccagcg gtgatgagaa aaaaataatt cctgcaatga ttatagctat aatgctaaaa
    27781 agcagtacac atttcattac tggaaatctt gcacgcccag aatgctggtg tttgtgcatt
    27841 gcatgttttt ttggtggttg tatggtgata aaatctttaa tcctagcttg cttttgaaat
    27901 ttttcaaaat tatcagtttc atgtacggtt ttaataatct ttgtaatttt tttattgttg
    27961 ggcgatagtt taatgatatc tacatatccg ctacaggcat catcttttgt ttttgcagct
    28021 ttcaatataa gaaatgcctt aatctctaaa agaggaatat agttttgatg ttgtcggagt
    28081 gctttccgta atgttgcttc ggcttgcacc agtttatctg ttaatagata tgctattaca
    28141 agcaaaaaaa aatcataggg atcatttgag atagcaatat ttttttctaa tagttgtata
    28201 acaacggaag tttccccttt tttaaatgct ttatatgctt ctttcgttat tatatttgat
    28261 ttcacattgt agttcttaat aataaatttt tatagagtat aactatttat gctacaatag
    28321 ttatctttta accctttcca catactcacc ttttcgtgta tcaactttaa tagtatcgcc
    28381 ttcatttaca aatattggaa cctgtatagt tgcccctgtc tcaacagtta tcggtttcaa
    28441 cacattggtt gcagtatcac cttttacccc gggttcagca taagtcactt ttaaaactac
    28501 aaatattggc ggttcaattg acaatatggc atcattatgc atcgttgctt caacattatc
    28561 tccttcttta atataattaa gcaggttccc tgccttcttt ttatcaatac ttaactgatc
    28621 atatgtttcc gtgtccataa aaatcagcgc atcaccttca tcatataaaa actgtaaagt
    28681 ctttttctct attcttacat cttcaacctt ttcgccacct ttaaacttct tatcaacaat
    28741 agtacctttt gtaatggatt tcaactttgt ttgtacaaac gcaccgccac gacccaattt
    28801 tacatgctga taatcaacaa cagaatataa atcaccatca actttgatta ctgttccgtt
    28861 tcgtaaatca ttacttgtta tcattacaac tcctccgaaa atagaattac acctcaatgt
    28921 cattccggac ctgttcagga atcccaatag gtatcgttat atcctgaaat cagtccatga
    28981 tatgattcag tatgactgcc aacaaatatt ttcatccctt attcccgccc atcaacaact
    29041 cattcactat gtttacattt gcgtatgctt tttaccacaa aaaataatta tacaaaacac
    29101 tgctatgcac catattaaag aactattata tcttttgggc tctttgttaa tacctcatat
    29161 ccattgtttg ttattaaaac catgtcttca atacgtactc caccacgttt tggtaaatag
    29221 ataccaggct cgatggttat caccatcccc ttttttaatt tgatcatgcc attttgctta
    29281 atggccggaa tctcatgaac atccattcct actccatgac caagtgaatg cccaaactta
    29341 tctgcatatc catgagcagc tataaaatcc cgtgcaatac tgtcaagttt atttgtagtc
    29401 attcctggtt ttactgctgc aatggcattc tcttgagctt tttttactat tacgtaaata
    29461 ttttttatat cttctgaagc actatgaaca aaaaccgtcc gtgtaatatc agaacaatat
    29521 ccattgtaaa tacatcccat atcaataagg acgggatcac cttctttaat tatttttaca
    29581 tcagtcttat aatgaggcat tgaagaacca ttaccagatg ctacaatagt tgcaaatgat
    29641 gttcctaaac aaccattttt tttataaaaa tattctattt ctgttgcaac atcccattca
    29701 gtaattccag gtttaagtat atttaaaata tgattaaaac ataaatcagt aagttttgca
    29761 gcttgcttta taatttcaat ctcttcctct tcttttataa cacgcaattc attgatacta
    29821 tctccaccat attctagtgc aatccctctg caatgttctt tcattgcctt atattgcgat
    29881 aaagaaatag catgttcttc caaaaagagc tttttaacgc ttttcttttt taacaatgcc
    29941 gatagtgttt gatttatatc tttttcctgt aacacaaagc gcacgttttg aggcaaaatc
    30001 gaaatagcat attcttcata tctcgaatca gatataaaaa cagtttcatc aggaaaaaaa
    30061 attaaatatc cgtaactccc atcaaatcca gtgcagtatt tgatattgat aagactactt
    30121 accagataag gaaaacagtc attgtttttt aatttgtttc ttatcttttt tattctggac
    30181 agatagttca tactattaac attatttttg aatattgttt agataatata ccagtgcttc
    30241 tattcccaat atatatgagt aataaccaaa tccgcatatc tgcccaatgc aaacaggtgc
    30301 tatgtacgaa tgttttcgga aatgttcccg tgcatgtata tttgatagat gaacctctat
    30361 gaccggtgta ttacatccgg ctatacaatc acgaattgca acggatgtat gcgtataccc
    30421 acctggattt atcataatgc catcacaatt tttatttctt tgaatataat ctataatttc
    30481 accttcactg tttgtctgaa aacaatgtat ggttatatta tgcgactttg catattcaat
    30541 aattttatta ttgatttcat caagtgtaaa aacaccatat ataccaggct ctcttgtccc
    30601 aagcatattt aagtttggac catgaactac atggaaagtg tattgtttca tatattcctc
    30661 gtagaaacgc aaatcatgca aattatacat atactatcat tatcgaacac acatcaaaaa
    30721 ccataatgtt taataagatg agttgccttt atttttgcaa gaatatttcc ctcattatta
    30781 actatctcat aaagaatagt ctttgactca tcttttttcc ctgtttcaaa aagaagcatt
    30841 gatacataaa tcttagcatt ataatagttt atatcatcct gttcagcttt ttcaaaatag
    30901 cgatcaaaat ttctttttgc ggtaacaaaa ttcttttgta tcgcaaaata aatgatccct
    30961 agataatatc gtgaagatat accatattca tcctgaaagc gtgaagcaat agtaaaataa
    31021 ttaatagcct gattataatt attattaata taatgtatca aaccataata ataattagaa
    31081 actgaaataa gtttttttcg tttactgcta tatgaataaa taatctttaa attttttata
    31141 tcattctgtt tataataatg ttgcagtaac aaagtaaata tttcatctgc aaaagcggtc
    31201 atatttatat catttaattt attgttaata atgcttaaag cttcatttat attcccaagt
    31261 ttataatgtg ctatgaatgc tatactatca cgcataatca tattttcttt atcctgtata
    31321 ttttttacta attctagcgc attatcaaac tgttcctgct ctaaataaat ctgagctaat
    31381 tgtatttgtg cattgctcaa caccatgcca tggtgtttct ttattaagac ctttttgaaa
    31441 tattcggttg ctaaatcaat attgccattg ttcttatagt ataaagcaat attgtaagca
    31501 acttcattat accgattgtc ctgctccata tactgtaatg tttcaatagc aatatcaggt
    31561 tgattattct ttaatgctgt atcagaaatc aaaactaaat catccatact ataatccatg
    31621 agtggtagtt tcataaacgt gtttaatgca atggtgtaat ccttcagcat ataataactc
    31681 tttgctaacc atttaatcaa taattttgtt gtataatcat caacagctat atattcacta
    31741 tccttcagcg tcttaattat tgtgttgtac tgtttcaact catataaaga aatgatataa
    31801 aacagtaaaa ccgaacgctc tcttggtctt ccttttattg atagatattg tgagtaaaaa
    31861 tatgaagcac tctgataatt tttcttttta tatgcttcgt ctcctttttc tatatatatc
    31921 ttaccggcag taatagacga atagtaattt ttaataatga agttccagta tttctcaaca
    31981 tttaccccct tctcatttaa tgatagcgac aactcatata aaatctcgtc caacccgttt
    32041 tttttgggga actttgctat taactggttt attgtctcaa ctgctttttt aaaattatta
    32101 atttttatat atgatctcga taatattatt aaaacttcat atctgttatc aactttataa
    32161 ttatttaaat atctgttaca gcttgcaatt gcactggtat aatcaccgcg tttaaaaaaa
    32221 tcaacagcct gggcataatc aagctcaaca agatattttg aaaatggatt aacttcttta
    32281 attagctcaa aatattcaag tgacttttca acatctccct gcaaaatata tatttttgct
    32341 tcaagataga gcatttcatc cctattttct tcataaggat atttttccag cataaaatca
    32401 agtaaacgtt tggcatcatc atagcgatct gtagcgatat ataattcgct cagtgtcatc
    32461 ataacgtact tttgactttc ctgatcatta attgaaatag cattttcaag ataaggaatt
    32521 gctttttcga ttgtattatt ttttaaatac aattgtccta attcaaatga agccctgctc
    32581 ctcaaatcga tatcattgct gtttacctgc acatcagcta aaagtgatat gccttcatct
    32641 aaatcctttg tatttagcaa tatttttcct aattcatacc gtgcattata ataatagggt
    32701 gaatcttcat tagaaattaa aatatacgat ttaagattta accgtgcatt atagatatca
    32761 ttcagtgcaa tatatgataa agctttaaaa tactgtacac ttaaaaaagc aggtatttta
    32821 atcattgatt cgggaatttc atttaaatat ttaattgcct gccaatgctt accctgtatc
    32881 tgataaatcc ttccaacgat aagctgtgct tttgatgaaa tagttgcatc attcaccgca
    32941 tgaattaatg gttcaagaat atccagtgct tctgaataac gttcactaag aaaaagagct
    33001 tcaccaattt ttatcaccct gttactatct tcaaactttt tactggttaa tgttaatgct
    33061 tttctccatt caattatggc ttcatcatac cttttttgat taaaatatat ctcacctata
    33121 cgatcatatg aatttataat aagctgctta tttttatttt gagtaataaa tctgttatac
    33181 tcttcaatgg cttttatata atcatttaaa tagtaatatg attcagcaat ccagtaacgg
    33241 gcttcattac ataaatcagg ggttgtacac aaaagaagaa atcggttcaa ctcaaatata
    33301 gcagaacgat attgtttctg atagaaaatc gataatgcta aataatacca tgctctatcc
    33361 ttatatgaat cattggcatc aataattttt ctgaagatta actccgaaga cccataattc
    33421 cccatcctga aagcttccat accctgttca aacagttggg agctttcaag ggcaaaagcc
    33481 agtgaacaca ctatactgtg cagtaaaata acaagtacaa ttacccgtct tttcattctt
    33541 attcaatccc cgcaaatgtt aacagtttat ttaaaatata attggctgct aacgttctga
    33601 catgctccct attaccacta aactggattt gtgttgaaaa agtatcgcta cctgctatac
    33661 caaaacatac cagacctacc ggtttttcct ttgtcccacc atccggtccg gctattcccg
    33721 ttgttgcaat accaaaatca gatgcaaacc tttgtttaac tccccatgcc atttccagtg
    33781 ctgtttctct gcttacagca ccaaacctat tcaatgtatc tactttaaca cccaatagag
    33841 ttttctttgc ttcattgcta taggcaatta caccacctgc aaatatgctg gatgcacctg
    33901 gaatatcggt aatccgtttg gatataagcc ctccagtaca tgattcggcg acagctactc
    33961 ttaatttatt ttttgccaat aaatgtacaa ctttttcttc cagtgaatta tatccaaaaa
    34021 tcagttttga accaaacctt ttttttattt catcaacaat atcaacggca ggtaatacat
    34081 tgccactttt aggactcagt ataactgtcg ttgtacctga ttcataggtt attgcaatat
    34141 caatattttc ctgtttattt acaatttctt taacagccga atcaacctcc gcttcacgca
    34201 tcataataac tttaaaggta agctcttcct ttttttgtaa ttgatatttt ttcttacaat
    34261 attctataac actcatcatc attgactgca actcgtgagg tactccaggc agtacaataa
    34321 tatctttatt gtcataccca atagcaaaac caggcgcaag cccattctca tttggaaaaa
    34381 cataagcatt ttccggtact gttaccatct ttacatcggt atcaagagca gacttgccag
    34441 tggctttaaa gaaataatcc atccttgctt ttgagggttt gtgtacagtc aaattcagat
    34501 gaaacatgct ctgcaaagtt tcacaggtta tatcatcaac cgtaggaccc aatccacctg
    34561 ttatgaagac gatatcagca ctatacatag catgttcaat aattgaccgc aaggatgcac
    34621 tatcatcgcc cacaacataa tgagcaacaa caggtaaccc tatagaaaac aattgcctgc
    34681 taatatagta actattactt tcatgaatac ggccattgac caattcactg cctgtagcaa
    34741 tcaaagcaac ttttgtcatt ataattattc tattactata gattagtttg gtgtaattca
    34801 accaatgtta taacaaaaaa tcaataaatt attttatttt tattacatac gttcaggggc
    34861 acagacccct aataatgaaa gagcattttt taaaatgata cgcactccat cacataatat
    34921 cagatagctt tgtgtcctat catggtcatc tgacagaacc ctgtgttcag tataaaactt
    34981 atggtatgct tgagcaagcc tcatacaata cacggtgatt cgatgaggtt cataactatt
    35041 ggcggcatca tatatttctt ctggaaaacg agccatcagc tttaataatg ttatggtttc
    35101 aggattacta taatgcgcag tatcaacaat tattttatca tagttgatat ttctgttttg
    35161 tgcttctctg aatattgagc atatacgggc atgagcgtat tgcaggtaaa agaccggatt
    35221 ttcactgctc tgacgtttag ctaaatcaat atcaaagtcc agatgactgt ccattgaacg
    35281 cattacaaaa aaatatcgtg caacatctgt accaatatca tcaataagat cgtgcattgt
    35341 tgaaaatgtt cccaaccgtt ttgacatctt tactgcttca ccgttggtta gcaaattaac
    35401 ctgctgtgat attaatattc taaattgatt ttcatcatac cccattgcct tcatcgctcc
    35461 aacaagacga gcaatatagc catggtgatc gggaccccat atatcaataa tcagatcaaa
    35521 tccccgttca atcttcgttc ggtggtaggc aatatctgcc attaaatagg ttggtctgcc
    35581 atcatcgcga ataaccaccc tgtctttatc atcaccaaac tttgtggaaa caaaaacctt
    35641 tttaccattg tcatcggtaa taacaccaag cgactcaaga tagttaaaaa catccagcac
    35701 cttgccactt tcatgcaagg ttgattcgta aaaccatgta tcaaaatgta cattaaaccg
    35761 ttcaaggtcc tgcttttgcc atgcaatatt ataatctaca gctttgcggg cacaaaattg
    35821 cacagccttt tcttcatcgt ttatgtttga tagttcatgc gaaaaatttt catgtatata
    35881 ttttgcaata tccttaatat attcaccatg gtagccttcg tcagggaata tcacatcctt
    35941 tcccagcatt tgctggtaac gaacccacac tgattttccc agccaccata cctgattgcc
    36001 ataatcatta acataaaatt cccggttcac tgtatcacca cttgctgcaa gcaaatttgc
    36061 cagagtatcg ccaacggctg ctgcacgtgc cgaaactaca tttaaaggac ctgttggatt
    36121 agctgataca aattcaatgt ttaccttacg cggatattct tttttatttc taccataatc
    36181 atcattagca gtaactatcg cctgcaaacg ctgaaccaga aatggcaatg ataaaaacat
    36241 atttaaaaag ccaggtttta ccacatcgat gcgttcaaaa atatgctgtt catcaataaa
    36301 tccttttatc tgttctccta tttgcagcgg tgaagtcttt aaaattctgg cacattctaa
    36361 tgcaattggc gtagcataat caccaaattt ttcttctttt ggatattcta ttttgattga
    36421 aagcgatggt tgttgtaata tgtcatgtgc ctgtaatacc ttttgcaaag ctttatcaac
    36481 aatctgtgca atatactctt tgattatcaa tgtgatgcct ccgctaaaaa atcgatataa
    36541 cttcactcat atattcatag catagataag aataatctta tttctcatcc atttttaatt
    36601 agttttcttt atctaccaaa tattttgtca agaatataat aaaaaacttg cattatttac
    36661 agcaatcccg tatatactat cttatataga gacactttct ttaatgttac ttcatagcaa
    36721 aggtagtata acatgcaaat taataaaagt tacctccttg gtttaggctt atcaattgca
    36781 gtatttttaa ttatctcatt tttttatgca caaaccaaac ttcttgatgg tcttgaatac
    36841 ggatcaataa actttcgctt ctttttgcgt gatccttctg aaaaagcaat aaagctacag
    36901 gaaggagtca aaatgacccg caaaaatccc cgttcacgtg atgatataat catccttgga
    36961 attgatgaaa atacaatacg tgaatttgac aatgaaggaa ttaaatggcc attcccatgg
    37021 ttatatcatg caaaatttac tcaatacgtt gcatcaggca atccggtcgc tatatttttt
    37081 gatataatgt ttttagatca caaaccatat gaagatgaat ttgccaaagc tatcaaacag
    37141 gcaaactgtg tttttttaga ttatcccttt gaaacagaag aagtagatgt aaaatactct
    37201 gatatagaag aaagaatgaa cctgttgaac atttacgcga tgacaattga tcccaatgac
    37261 aactccatgc catgggtaga agaagctgtt cccccaactc ctctattgtc tcaagctgca
    37321 attggtctgg gttttgctaa tataaaacct gattctgata atattaatag aaaggttccg
    37381 ctggttgtta aatataaagg tctgtattat ccatctattg acctcttgct ggtaatgcac
    37441 tattatggca tatcaaaaaa agatgtagaa ataaaagtag gacaatatat aaaactatcg
    37501 aacataccaa aagaaaaaat ggtgaaaaca actcatgata acagcataaa aatccctatt
    37561 gatgcggaag gtttcatgga catcaacttt attggaggtc ctggaagttt tacccattat
    37621 ccttattatt atttctgccg agatggaata attaccaata cctctttaca ggataaaata
    37681 attttggtag ctgcatattc gtcaacaggt atttcaactg atatccataa atctccttat
    37741 ggcgatcttt ttggcattga gcatcatgcc aatgcattaa ataccatcct taatcaggac
    37801 tttatagtaa agctaacacc tttccaaaat atacttatat tgtttgcaat tgccatacta
    37861 ttaggattta ttttgaccag ggtatctatc ataatttctg tcttatttac tgtaggttta
    37921 tcactgttat atattattgg ttcatattta ctatttgatg cgtttaatat cataacaata
    37981 tttgcatctc ctattataca ggttggtaca acattttcaa tcattatcgc ttacagagtt
    38041 ttaaccgaac agcaggaaaa gaaatatata cgacagacct tctcaaaatt tgtatcaaaa
    38101 tcagttgttg atgaacttct taaaaatcct gaaaatctta aattaggtgg tgaaaaaaaa
    38161 ttcctcactg ttcttttttc tgacatacga agttttacta caatttcaga aaaaatgaca
    38221 ccagaagagc ttgttgaaca ccttaatgaa tatttacagg caatgacaga actggttttt
    38281 aaatatgatg gcacacttga taaatatgtt ggtgatgaaa ttatggcttt ctggggagcg
    38341 cctataccac aggacaacca tgcacttatg gcttgtaaat gtgccgttga aatgatacag
    38401 gtacttgaac aattgaataa acgctggatt cagatgaaca aaccagccct tcatattggt
    38461 attggtataa acacaggtga aatggttgta ggaaatatgg gatcggcatc tcgaatggat
    38521 tacaccctta tgggtgataa cgtcaattta ggggcacgcc ttgaaggaac aaacaaacaa
    38581 tatcaaacca acatcatcat cagcgaattt acctatgaac aagtgaaaga ttatataata
    38641 gcacgagaac tagatttagt acgtgttaaa ggtaagcaac tccccgtaaa gatatatgaa
    38701 ttaattgaca tcaaacaata atatttgttc tttaccacag ggaaaagatg atacaataaa
    38761 aggacgtaac agctcacctt ttattgaaaa tgatgctcta tgagtagtgg taattgttct
    38821 tccgtctctt ttataaaaag tagcattaca tggcaatgat aggccatttt caattgagta
    38881 gtttccatac ccactcacgg aagcattatc tgtatcgata tacaaacttt gcaaaattaa
    38941 tctgtctgca gtatgattga ttgcaacatc aattcgatta caatgccacc ctcctccctc
    39001 aacaactata ttacagcttt tagctaactc tataattttt ctttgaatca tggatgaatt
    39061 taaaacgcta tttgctatat tcaatgtaaa attgccgttc ccattttgca acagatgact
    39121 taaacggtag cccgatacac cataattcaa attcatgttc attaaacctg ttgcatcttt
    39181 caaaccagta tcacgtaaaa atgtaccata gtcaaaatta tttaccgcac atgaaattgt
    39241 cattgtagga taatcactat tgcagaacgc attaacacta aaattatagt tggcggaata
    39301 accctcacaa ttgaatgatt gagtggttac caaacctttg ttagatagta actctatatt
    39361 aatgtttttc agatgagcat tcttttcaaa tgtcaatgta tctacattga tttttgtatt
    39421 gattatattg tttgatagat atttccccaa aggtttttgc ctgaaataaa tttcgttata
    39481 gccaatactc aaatcttctg cagctttgga gtataattct tttatgcctt tcaataaaag
    39541 tttggataac aattcagcac tcaatctttt tgattgtacc ataatttcat cagttgaggt
    39601 aaaaggaacc cacttctcaa tattaatagt tgatcgtaac atcacatctg atttctgatg
    39661 tttagcgcag gcattaatat ttattttctt tatatcgcca ttaattttta accatgaatc
    39721 cttaatatat gtcatagcat tgtttttggt tttactgaag attgcgatat cttc
//
LOCUS       DAOOJH010000017        34876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1352_length_34876_cov_9.644755, whole genome shotgun sequence.
ACCESSION   DAOOJH010000017 DAOOJH010000000
VERSION     DAOOJH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 34876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34876
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1352_length_34876_cov_9.644755"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="PLJ75_04135"
     CDS             <1..1284
                     /locus_tag="PLJ75_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HPD04435.1"
                     /translation="VDVVICLSHSGIKYYKNKKEIDPDRSEDVQLAKVVNGIDVIISG
                     HTHTKITRPLIVNKTIITQSYEYGKQVAVLDCTVSKDGVSLKDYKYIDINDSIKGDAA
                     ITAMIQQFTQVINQRVLSPMKLQWNSILAKTTFDLTLKEDECNLGNLIADSIRWYVNK
                     VDANPDDPDSKVAMTVISGGVIRDNILVGKTGKVALCDAFAAIPLGIGMDDTMGYPLI
                     TCYLYASEVKKALEILTSIYPLKGSDYFIHISGVKFTYNPNRMLFDRVTEIWIGDEHA
                     GYKLLDYSSNNKKLYRVSADIYNATFLKIIGNFTWHILDIVPKWRDGKPIESLKDARV
                     DADKKKVGIQELKEWQGVIAYIQSFKDEDGDGIPDIPAMYKDKLGRIVVEASLNPYSM
                     LKRGTTVTWIGFVVFLFILAIVGGIVYAVVKRMRR"
     gene            1326..1523
                     /locus_tag="PLJ75_04140"
     CDS             1326..1523
                     /locus_tag="PLJ75_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04436.1"
                     /translation="MVLVESTIIIYPAMNPGGVFNACALQEKGADEIMSLEKEAYNDA
                     IIKKFRNNIIHAQKSFKRTAL"
     gene            1489..>1753
                     /locus_tag="PLJ75_04145"
     CDS             1489..>1753
                     /locus_tag="PLJ75_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04437.1"
                     /translation="MRRKVLKEQHYKKALDKTNRAVAYLYGCGAQAVYIFGSLLNNTM
                     FDEMSDVDIYVEGLPREKKKGLFSALDELFGEIKFDLFFDDDVL"
     assembly_gap    1754..1853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1958..3502
                     /locus_tag="PLJ75_04150"
     CDS             1958..3502
                     /locus_tag="PLJ75_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPD04438.1"
                     /translation="MHKHTEEGTMLSRATSLVIHGIEAHLIDVEVDIVKGLPNLTIVG
                     LPDSIIRESKDRIRSAIENSGFEFPPKNFIVNLAPAGYRKEGANLDLPIAVSILVATG
                     QVESTQLTIPMVGELSLDGRVRPIKGVIAMAIALYHAGIKEIIVPYENRNEATAIEGI
                     KVYPVKDIAQAIEACNGTMQPYTEHVSGDSSWNGYLDMASVMGQETAKRALEIAAAGR
                     HNILMYGPPGSGKTMLARCVPGILPPLTKEQAIMTTMIHSVGGVLTHGQGLVYMPPFR
                     APHHTSSDAALVGGGKIPNVGEVSLAHNGVLFLDEFVEFRNDVIQALRQPLEDCQITI
                     SRALGTMTFPADFMLVASSNPCQCGYLFDPEIPCKCTAGKIRNYFSKIAGPILDRIDI
                     EVLVGRVPYKDLLGKSNAESSARVRERVVKAREKQRQRFSGRTTTCNARMTSDEVKEF
                     CAINGECESILELAIKRMNLSARSFFRILKVARTITDLDDTESIEKRHILEALSYKNL
                     QRYYEI"
     gene            3563..4438
                     /locus_tag="PLJ75_04155"
     CDS             3563..4438
                     /locus_tag="PLJ75_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPD04439.1"
                     /translation="MYSIIQGRLSIWIVYALLCALFLAIGDALVKNAPAMHPVTIAAG
                     RVFFSLPVLWAIALINGIPAINSDIIYSLLLSPPLEAIALVLYMRALQISPMYRTLPF
                     LSFTPLLLIITSWVLLHERASLSGLIGIVCVVLGTYSLYFQRGQSLLVPLRSVFHDRG
                     SLMMLIVAVLYSITANFGKMGVVYSSPAFFGAVYFTILFIILGIWCAYADSIKNIFTK
                     NIVAIGITQGIMITFHMLAVAIAPVSYMVAVKRSSMLIGIIFGYVFFGEKNLLQHGIA
                     ALIILTGIGIISIAG"
     gene            4754..4957
                     /locus_tag="PLJ75_04160"
     CDS             4754..4957
                     /locus_tag="PLJ75_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04440.1"
                     /translation="MENTVCSSCINRNICSKESLAHCEHYDVVLTRAEVVDLLKNTRE
                     QDPIKFQSILNGIKKTYHINLAY"
     gene            complement(5050..6009)
                     /locus_tag="PLJ75_04165"
     CDS             complement(5050..6009)
                     /locus_tag="PLJ75_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04441.1"
                     /translation="MQAIVTIENLTIAFTSMYGKNYAVRDLSLQIPRAKTLGLVGESG
                     CGKSVTAYSLLQLVPKPGVIEKGAIMYNTLNLLQCPDATLQSIRGKNISMIFQEPMTS
                     LNPVFTIGYQVQEAVELHLHLQKAEAKDYTIALLGKAGIPSPSSRYYSYPHELSGGLR
                     QRAMIAMALAAKPELLIADEPTTALDVTIQSQILDLLLTLQKSQHMSMLLITHDLGIV
                     ANVADIIAIMYAGEIVEIAPTREIFNNFLHPYTQGLMSCIPSLHKKSKRLATIPGTVP
                     IITKPPSGCVFYPRCYRGDADCLHAPVSLLQKNRNHFVRCIKA"
     gene            complement(6009..6290)
                     /locus_tag="PLJ75_04170"
     CDS             complement(6009..6290)
                     /locus_tag="PLJ75_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04442.1"
                     /translation="MQREILFDSIHEFINTLRTNDITKVAFTQINEKRAREVAPGTLQ
                     VVDVATAEILAYKDATIYKYKRDNADLPQLYAMLQSQGFELIKRSRNIV"
     gene            complement(6305..7111)
                     /locus_tag="PLJ75_04175"
     CDS             complement(6305..7111)
                     /locus_tag="PLJ75_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04443.1"
                     /translation="MKRLLYPLNCLCTLLLLLVTACSVQKNITTQPIRFVIINNTSPG
                     SPYAAYDAQLATIIQSINNENPVFTIHSGNIVHGGTIAMGIHEKDIETQYKQFYRYCS
                     KLNSILFTACGPQDTYNNSTRLYTRFTQKQAWYSFNYGSHHFIVLYSEDNNVLKLPQQ
                     ELQWLHDDVKQNQHASKVVFCYFLPAIHKNNSQTFIHNDYQHLHTMLKQYNVIAFIGV
                     TGISSPVTIDTIQYIPVPCDNTFTNKYKKAIRYYIVDFNGNYIKLVPRYL"
     gene            7842..9743
                     /locus_tag="PLJ75_04180"
     CDS             7842..9743
                     /locus_tag="PLJ75_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD04444.1"
                     /translation="MADRWYITQQTFPELLNRNVIKYGTRRAQWWKRGDTTHSINYAE
                     LGFIVKQLTAGLMSLGIQKGDRVAIASHTAPQWMWADYSILCAGGITVCIYPTLSAKE
                     MSFIINDSGSKILYVQDEEILQRALSVADEMPTLQKCIIMKDDYKGSDSRVLTLPQVR
                     ELGIQLLAKDKLAYESRWRSVDILDPMTIVYTSGTTGRQKGVVHTHFSINAACCRDLR
                     IVPEYREDDIMLSFLPLAHTYERECGHGTAMMAAVTIAYSSPMTLIEDLKVFKPTVFM
                     SVPRIYERVFMAMRDMASKSPVTKKIFEFAIKTGLQLTEARSDENGFVDMSEGINFTE
                     GVGKWLAFKYKLVDKIIFSKVRETLGGRFRFAFSAAGSLSADLCKVFMAMGVRIYEGY
                     GATETCNTINLNLPHKVLPGYVGPVCNGVEGRIASDGEWQVRGNNIFTHYWNNPEATN
                     ETFTEDGFYKTGDIVQMGPDGYIKIVDRKKGIMVLDTGKNVPSAKIESLFSVSRYVDI
                     VFPIADDKPYVGALVVPKYDVFIELFKKEGIPYDQSKLVFFEEGATRTCVAVGDDFIQ
                     NELLRKLVDEDIQQANKELEEYERIKKYVIVNRKFSESNGEMTPTLKVKRNVVLKNFE
                     KEIQELYKK"
     gene            complement(9814..10671)
                     /gene="argB"
                     /locus_tag="PLJ75_04185"
     CDS             complement(9814..10671)
                     /gene="argB"
                     /locus_tag="PLJ75_04185"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPD04445.1"
                     /translation="MKQYIEKVNTLIESFPYIKEFYGKTIVIKYGGHAMETLEGKRSF
                     ALDMVLLKYVGINPVIVHGGGPQINTLLEQVGVQSAFVGGMRVTDASTMEIVEMVLVG
                     KVNKEIVNCINLAGGRAVGLSGKDGNLLIAEKIYHTANGEKKDIGQVGTITKVNPLII
                     ETLDREKFIPVIAPIGCDNNGITYNINADIAAGKLAEALKAEKLILLTDVEGVKIDGK
                     LISTLRKDEVDNLIISNKISGGMIPKVNCCLGALKEGVGKTHIIDGRVEHAVLLEIFT
                     DAGIGTEIV"
     gene            complement(10668..12047)
                     /locus_tag="PLJ75_04190"
     CDS             complement(10668..12047)
                     /locus_tag="PLJ75_04190"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HPD04446.1"
                     /translation="MKIHYPGYETVINNVYDSGNEHVFAYWDTLNPDQKKHLLEELST
                     IHFETLQQLFELTKRNLPPLDFKPAPYTPLPETDEEKKEFEKAKQIGIATIKAGKVAA
                     FVVAGGQGSRLGFEGPKGMFPIGPVSKKSLFQIHAEKIRAYEIKYNVTIPWCIMTSEA
                     NHDATVAYFEQSNFFNLNKEKVFLFKQNMIPSLDENGKLILESPCSIFRNPDGHGGSL
                     TALYQSGTLDTLQALTIEILSYFQVDNPLVNIIDPVFIGFHRMHSAEVSSKALVKAYP
                     EEKIGVFVQFSNNRIGVVEYSDLPPEKQYEKDEQGQLRYAAGSIAIHLFNTQFIASVT
                     GSNVTLPFHVAKKSIPKYTDKGIQHITGYKFEKFVFDALPLTQHNFILETKREEEFAP
                     VKNKTGVDSVESAQELMIHLHKRWLTQKGIAIPTMLKKIELSPLFAVEKDDIPQSLII
                     PDKEEVYIE"
     gene            complement(12433..13122)
                     /locus_tag="PLJ75_04195"
     CDS             complement(12433..13122)
                     /locus_tag="PLJ75_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04447.1"
                     /translation="MKYVYCIGIVIISMCMVLPVYADDLGIGISAWYADWEMDNSSNP
                     KKTMDPVLCIGPSLSYQFSETLSVTLVALFTPKKYEMPDEGSEDSKLRRYDSDLALNY
                     QLNRYIKLFAGAKYLGFTFENGKHHGAGPGMGIGFTIPIVYNFYFLGNASGLYLYGNH
                     NDNSNDTNYNEYGYNLAGQLAYYFPSIGLTTSLGYRYQYIKSDYNTNNMADDEHTFKG
                     FTCLVVKSFHL"
     gene            complement(13486..14673)
                     /locus_tag="PLJ75_04200"
     CDS             complement(13486..14673)
                     /locus_tag="PLJ75_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD04448.1"
                     /translation="MKLHVVTSNNVDNLLSDNVITLPPSGIREFFDIVYNTPDCISLG
                     VGEPDFVTPWRISDAGIYAIKDGNTHYTSNKGLQELRQHIAAIISKKHNVLYDPETQI
                     LVTNGVSEGLDIALRSIINPGDEVIYFEPCYVSYSATIKLAYGKAVAIPTHFDNGFAI
                     DIDQLQKTITHKTKAIMLNYPANPTGASIDKQTLEAIASIAIHHNLIVIADEIYDEIT
                     YNGNHFSIISIPGMPERTIYLNGFSKAYAMTGWRLGYACGSESIISAMNKIHQYTALC
                     APSIAQYAAIEAITHGQRDVATMRSHYVKRRNFISSRLQEIGLPCYPPRGAFYVFPSI
                     AKTGLSSREFAVRLLKSHKVAVVPGTAFGSWYDNHIRCSYATSIEQLKEAIDRIEDFV
                     NRL"
     gene            complement(14739..14960)
                     /locus_tag="PLJ75_04205"
     CDS             complement(14739..14960)
                     /locus_tag="PLJ75_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04449.1"
                     /translation="MEIVSYILNNTTMFVIIAGLLIACAGFLLLRLRIVSIVCIILAA
                     LIIYVLLYKTSPIPTQPTETREIDRIDKL"
     gene            complement(15053..16003)
                     /gene="glpX"
                     /locus_tag="PLJ75_04210"
     CDS             complement(15053..16003)
                     /gene="glpX"
                     /locus_tag="PLJ75_04210"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HPD04450.1"
                     /translation="MDRNLALEVVRVTEAAALWAARYYGRGNEDLAGKNAVEAMSKVF
                     STVSFKGHIICGIDNPDSSLCTGKIIGNAQHPEVDIALTPIDGHATLAQGGYNTISVI
                     ALAEYGSMLNAPPIYMEKIAVGKDARGVIDITQSPEVNIKRVARAKGKYIDDITVCIL
                     DRARHEELVSRIRALGARIKLIKDGDISGAVATALDDSPIDMLMGVGGAREGILSAAA
                     LKCLDGDMQARFIYRDEKDKELVRNMGITDIDKIYTLSDLAKGENIMFSATGISDGEL
                     LPGVRFMAGGAKTHSIVMRYKTHTIRYITAIHRFDYKPVY"
     gene            complement(16390..17547)
                     /locus_tag="PLJ75_04215"
     CDS             complement(16390..17547)
                     /locus_tag="PLJ75_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04451.1"
                     /translation="MHYIFSAIAILCLTTTAFAKGIPLDAVYMDKNSSEYQKLFFARL
                     NNYAQMGAQFIDSNIVYSAWCSGKEIVYIKESGINNTIVVCAITTRKKNPLYTFKGTV
                     ISARTSSNGRYIAVKYFTNQRIPQPALFIIDCIGGTVKLLQTLSAGNDFSFSDDGNIF
                     YYKKNNCIVAYRCDSSMEKAFLCSSDAPQWDEICTIIFNSNQLFVSGSSGNYTVYIKQ
                     KLWKKICNAVTPAEVFSDSFAIYYTGGYPGNYTVHRYSVKTQKHETLMQQSLNPCLQV
                     SRNHAIFLKNAFIMMYDSINGRLFTTTVESDEAIISPNGFYVASTIYNRLFIIPYATV
                     VTINPKAGRYLTTLQELYHDVVNKKYLHISPYTRTYIQQKQATIASLAKLF"
     gene            complement(17551..18825)
                     /locus_tag="PLJ75_04220"
     CDS             complement(17551..18825)
                     /locus_tag="PLJ75_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HPD04452.1"
                     /translation="MKHIMYYIIVLSIAFAMGCASDLPIRDMSKARYGITEAEEVKAP
                     QYAPDELEKAKQYLYDTHTLLKEDKTKDAQKKSVESYEQSQKAIEKSLPLYSSDMVTQ
                     AKNSISEIEMLNAREFAPAEYAQAVALATESSDLHNKTNYRESIQKSREAITSANDAK
                     VKSIAMIPQLKEQLVALEKERDELSNNRGDEFAKQQLQDASAKISEANTKLDEQNITA
                     SIDAMQQAKESLLAAKVAIDKGNALESLEAAKSLYQEVSQRETAQDFSEQLTQAEGLI
                     KTSEDKFKAEQYPESYDTSQQATNILNTMLIAMEKKEEAIAIQEEGEETKQETTTATT
                     TKEVTQQQKDYIVQYNPKARDCLWRIAMKVYNNARLWPLIYIANKDQIKDPDLIFPGQ
                     RFVIPPMEKEKQPIEESQSKTMDDKKPQDVKE"
     gene            19073..19639
                     /locus_tag="PLJ75_04225"
     CDS             19073..19639
                     /locus_tag="PLJ75_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPD04453.1"
                     /translation="MIESEVLVLNAGFIPIRITTVKEAICLLLSDKAVPVVEENTFVR
                     SPSIAIKVPSVIAILNYHQLPRRKVVFSKLNVIYRDDMQCQYCGRRFQMKQLTVDHVI
                     PRSRWEIVTGKSFKEGFSSWHNLVCACKWCNNQKGSRLMHEIGWSLLREPFEPEYIPY
                     IVVTAEKAKKKGWLPFCGFNVKIVEMIP"
     gene            complement(19700..21631)
                     /locus_tag="PLJ75_04230"
     CDS             complement(19700..21631)
                     /locus_tag="PLJ75_04230"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPD04454.1"
                     /translation="MGYSVCYKILRSHIQEGNLNAGDEIGITIDQTLTQDATGTMAYL
                     QFEAMGIDRVKTQLSVSYVDHNTLQVGFENADDHKYLQTMNARYGIYYSKAGNGICHQ
                     VHLERFAIPGKTLLGSDSHTPTAGGMGMLAMGAGGLDVAVAMGGGLFYLTCPQVINVY
                     LTGKLRAWVSAKDIILTILKMLTTRGNVGYVIEYTGPGAATLSVPERATITNMGAELG
                     VTTSIFPADKITLQFLQAQGRADDYVELLPDSDATYHKVIELDLSTVEPMIACPHSPD
                     NVVPVAAIKGMKVDQVAIGSCTNSSYKDLVTVATILKGKKVHPNVSCILAPGSKQVLQ
                     MLATEGYLADIIASGVRIMESACGFCIGNGGAPISGGVSLRTNNRNFEGRSGTKDAMV
                     YLVSPETAALSALSGTLQNPLDQPAERYPQVALPASFVIDDAMIIPPFQSNEPIFRGP
                     NIGQPPKTTPLPDAVKGVVGIKLGDNITTDHIMPAGQRLKYRSNIPEYAKYVFEHVDS
                     TFAHRALQNKDKGLATIIVAGHSYGQGSSREHAAICPAYLGIRMVIAKSFERIHTANL
                     INFGILPLVFVHESDYDSIQQGNEVVMDDCINQVKQVQNDTMVVTINGRAITCKLLAS
                     QRQRAIIIAGGLLNYTKGK"
     gene            complement(21654..22541)
                     /locus_tag="PLJ75_04235"
     CDS             complement(21654..22541)
                     /locus_tag="PLJ75_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04455.1"
                     /translation="MHITIVTDSGAHIGQGHLQRMLLLAWFLATRRRWSVTLVHASKA
                     YTVPSSLQSMVAASIPKQTTCILRDMRDSTEEEIQQLQAIAPVIVIDDCGKGRPLAFR
                     AIDLLPTLHTATSNSFAAGNDLFLYGYNFYESLQALPSTVEKDIDCFLYASTFDSQHL
                     IAAMGNKFTYVCSDGNKVISNTGCTSLQHGAHSIARSKVVITHFGITVYEAMLCNCNC
                     ILLNPTTYHQQLSLMLAYPGGKLHSLLYDEAAINDIVTRCRDFVSAAPAYTDIDCCRV
                     TIATNLKNFADALTLILNT"
     gene            complement(22543..23289)
                     /locus_tag="PLJ75_04240"
     CDS             complement(22543..23289)
                     /locus_tag="PLJ75_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HPD04456.1"
                     /translation="MTANNQSVIAIIQARMQSTRLPHKSVMPLAGKPLLAHVIERARA
                     ISAVNTVVLAIPDDSENDSLVTIAQQCNVSVFRGSMHNVLERFYRAYRRFGGSYIVRI
                     TADNPFTDAAYASMVIDTAIKLNTDLCAAKNLPLGTGVEVIKAQALERAYTQSTQPHH
                     FEHVTPYIKENPDIFAINYFDVPLHNPFSKLRLTVDTQDDYTLAKHLYDALYTGTVFS
                     LDDVIKYLEKNPALVSINAAVQQKPMTSHE"
     assembly_gap    23680..23779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23780..24346
                     /locus_tag="PLJ75_04245"
     CDS             <23780..24346
                     /locus_tag="PLJ75_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04457.1"
                     /translation="QKRTEERVEELAQAQKRTEESIKELTRAQRENEKILKNFMVAFD
                     DLKKQVGGLSMVVGYGIEDDLMPLMPQFVQNTYKVKSCEVERKYILYDDGNYDEVNLH
                     IRAKRNGKDVYIIGECKAQPGKKDIDRFDKMLKRIKNHYKSDIYGFIVGYTYHPEIEE
                     YCKKHAIDIFKTYEIKRLAEVKKRNKKQ"
     gene            24573..24854
                     /locus_tag="PLJ75_04250"
     CDS             24573..24854
                     /locus_tag="PLJ75_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HPD04458.1"
                     /translation="MIATQHFLQRAHNRGITNMELQLVQMYGYQKNDCIILDRKQTRD
                     MLDELNKLRRVLINIYEKGGISMVVQDDSLITTYPINRRINKKKAKYHV"
     gene            25032..25667
                     /locus_tag="PLJ75_04255"
     CDS             25032..25667
                     /locus_tag="PLJ75_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD04459.1"
                     /translation="MKKQRLYILIIMMINNMIGNKSKEELLKENFEYIDKSIRNVNNK
                     YNYNLTDEDREDILQQICLKLYETGFDNFQWQCKVTTYIFKIVENEIRSYVRKRNKII
                     VSTDLRKHDEQYNNNITEDFINNISYTIDFAQQLTNDEVAKIIKDTVDAMPALQQLIF
                     KLHFVDGIKQEDVAKMCKRSQATIAEHIGNIRKSIKQEVEKKFKGFIKELQ"
     gene            25669..26235
                     /locus_tag="PLJ75_04260"
     CDS             25669..26235
                     /locus_tag="PLJ75_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04460.1"
                     /translation="MGHLGNDLQCSSQMEELVLGVADGTITDNAIIDEVLEHLVHCNY
                     CYELYKDYFIIAQPLLTEQHGDDTLTLDIDYDNGFIPLNVNAQSFAAQNVVAVLSKQK
                     EPVIEFEIPLADGALTVRIIHVDGVFDIVVSNSNNSNGAKIFLLTDEKFEVATLSNNR
                     ATFTFIKPGIITLIYNFKKIITIKLNCK"
     gene            26296..28311
                     /locus_tag="PLJ75_04265"
     CDS             26296..28311
                     /locus_tag="PLJ75_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HPD04461.1"
                     /translation="MSIIYIDESGAFDQYQKKRNVIGGIWCKMPVPHNEVKQLFSQCG
                     LSKVHACELDNETLSLLVQKLIPFCEGHSIKPVILLPRRGFFIISDTATYINVLSDGL
                     VEFIKTYAKELDATIEIIIEHRKGFKVDVDTKRLHEAIAKGVALSKVQNNINYTITFR
                     GKDNIFLQLADVVVHSFYRLDHDGSFLKGEVKEAFKEWMEPNTVYIYGNESYVTRIYD
                     CLRKGDYGEALQLYANHYMIPEVRQQKDFIIKNILKLSQPTMAVVLKNILTLYYDKVN
                     TNRLLNEYEPLVKLWIYTILPDIEKAIADGNNTSTAVDDLYWAYGELYQILLTIYNHK
                     GQIADFETTYNEARILLKKAVYDLDSLSVRIRIQVLNAVHYINQYQFEQAYTSMKVLA
                     KRVEDAFVFLSEADNDVKVKPRILGEIYGTMVQACMYHTLVTKNNFNEARELSNKALH
                     LFSHTDDIQRQYQYRAQIETYDGNYEQAMQYIAKGVGCTTYSDDDLIAQCLKNKSMFG
                     LQHLLRIWYCKLMNGYENTYYTVLSNINKPGSTIAKNYKEIMEHKVYPVHSIYRCVIV
                     IYGLLQSKHWEEHINKLLPRAKELFDGQPLTMQTLQLGLLADVLWIFAHHDAENKEIM
                     DRFNKRFNDVMHEASKYAIGDYLQKAYQHYQQTPVKKWNTLWWVFPF"
     gene            complement(28357..29925)
                     /locus_tag="PLJ75_04270"
     CDS             complement(28357..29925)
                     /locus_tag="PLJ75_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HPD04462.1"
                     /translation="MTLTKQWNNLPKKKRYSFYTEEILPVISQSLHNQLSHYLLSEHA
                     KPYSTVITILSPNIHPVLLMLNAILPHHCVLLYTPEKEQLLQQIKHHCATRGIELHPV
                     ELSPTNHQDNITIMQRTIKEFSKKFPPVLCDITSGKKIMSTQIGIIASSLKLDIAYID
                     SNKQYLESGIPQPGSEILYIQKASTIIEFSVHDNVLRIAYDDNNNTMRYECYFNDCSY
                     KLGTKKIHSNDIEQLSNLLSSCRDDINYSISHSKNYTVLHTQSHVLTLCSLLFSRELT
                     TFLKTNCPISNMRIIADEEVASIPWELVLSHYGIQLPIVRIPNRDKEFSEPSVYSSNK
                     KIIIIFGSSNNIPDFNKFEQNIKMLASSHNAYNITTVEAASASNLKVSISKHFPCAVI
                     IYYGHAVFSTTPETTGLVCRDGSIFSIDDCEIFHASPPQCMVVNACQSASGSLFLKNS
                     FAYAVLKMGVATYIGTNFFLEAQRSFVFIKTFLKNITSNTTYYNAYASALQELQKNFG
                     NDDIALYNYVYYGN"
     gene            complement(30049..30945)
                     /locus_tag="PLJ75_04275"
     CDS             complement(30049..30945)
                     /locus_tag="PLJ75_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HPD04463.1"
                     /translation="MHSNNDIYDFSQLKEVVLQLTHGKMEKPKTPLPVIQKTLNQDEL
                     LKNICFNEDGIFLQRNKDKIKGYIYIKKGYDIHYAKTNNIGTIVPKFHIYKCKTIEEQ
                     MEMNNFDGHYEFNNYPVKMKDIDGIEKELKLCKNCLNLSKNIKRVITVSEYCNNYINN
                     NKIGGTFGKDDLPLPIQRDYKNYTSNWVNISNELRKRKNYTCSHCKITIDSNFADRRF
                     IEVHHIDGNKANNHQDNLQVLCILCHAFMNKKHIGNYKKISNITRLSSFISRYEDKIK
                     KHNIKYYQKAVALCNTNRSLKS"
     gene            complement(30946..31656)
                     /locus_tag="PLJ75_04280"
     CDS             complement(30946..31656)
                     /locus_tag="PLJ75_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD04464.1"
                     /translation="MHKKSDHYWISMSDIMTGLMVIFMFISVAYILQLKQQQKERNQI
                     LEEYKNVKMALFNELQKEFENDFKKERWDAIIGEDLTIRFKNEKVLFDYNKSDLRPQF
                     KNILDNFFTRYFTILLKAPYKKHIAEVRIEGHTDSWGDYLYNLRLSYERTQSVLQYFF
                     ESPSSPYYTLNEDDKKIVRFWLTATGFSWGRVLDSDGNFVLESGKKENRMQSRRVEFK
                     IVTKSDAIVKTILDTMGN"
     gene            complement(31660..33093)
                     /locus_tag="PLJ75_04285"
     CDS             complement(31660..33093)
                     /locus_tag="PLJ75_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPD04465.1"
                     /translation="MIAITVIATLVILFFAMYSGYYIWQAYNKPQTINDIFLDSIPGI
                     FTTLGILGTFVGIFIALMNFDVNNITTSIPYLLGGLKTAFLTSILGIIFSLISSRFIE
                     NIQHTRKNQLYVDDELSVLKELLKLFADFKEHHTTKLDELITNIHGDNDNSIATYLIK
                     LRTSLHDNFDEQNKILESIHKNVGADTETSLLTQISKMRMEQSDFIKNTNDHVQSFKN
                     LLLESREFIGSKFEEFGKLLEKSNTEALVKAIENVIGGFNERLNELLQRLVKENFEEL
                     NQSVERLNQWQHENKEQVQTLINQFKTISSELGITSGNLEKISQYTKELIDGEGKLKA
                     LIQELEAVLTKNTMLVDSAKHLLQATEKMDKSAITLQNWMDMEKDLGQSIAKLIDNLK
                     EIENLRDTSGQFWADIKERMNETINFIEKGNMELLKKFEESDRYFYERLNKSFENLDA
                     VLQSMVTEYAERLDSILNPEGNNLYTE"
     gene            33441..34454
                     /locus_tag="PLJ75_04290"
     CDS             33441..34454
                     /locus_tag="PLJ75_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04466.1"
                     /translation="MLIIKKAYYYTIYEEETMKRYTTLLLILAVAGLLACGSNYVRTN
                     SSGSSYNYSYDERDFNKYKWSAWQRSQWVQNHNGWILNVKKCTYHNRWNGHTFAMWSF
                     DPKCESFYTAYQITPEMCHGENPKKFLWERGFRNIMYYEPANVDIEYLNPNVANFTRE
                     EKSFTTAGTVIGPRKIGVNKGTAIANAPEPLTPQEAAAKEREERKRIMAMQDYDKKAA
                     ELNNYIVQINKATRLLNAYLAATNQTLTEEEAAYQAWLVNAMIATCRADVYDKTSWYR
                     YEDGTIEHLQNYYCLHNLYSKFVFRFSIDLDGKNTEPNHLRQFCDEYWKKYHESEQAA
                     KNK"
BASE COUNT        10545 a         6195 c         7079 g        10857 t
ORIGIN      
        1 gtggatgttg ttatatgcct ttcgcacagc ggtataaagt attataaaaa taaaaaagaa
       61 atagatcctg accgctctga ggatgtgcaa ttggcaaagg tagtcaatgg tattgatgtt
      121 attatcagcg gtcatactca cacaaagatt acccggccgc ttattgtcaa taaaaccatc
      181 attacacaat catatgaata cggcaagcag gttgctgtgc tggattgcac tgtctcaaaa
      241 gatggggtat cgttaaaaga ctataagtat attgatataa acgatagtat aaaaggtgat
      301 gctgccatta ccgcgatgat tcagcaattt acacaggtaa tcaatcaacg ggtactatcg
      361 cccatgaaat tacaatggaa cagtatactt gctaaaacaa catttgatct taccttgaaa
      421 gaggatgagt gcaaccttgg caatctgatt gccgattcca tacgatggta tgttaataag
      481 gttgacgcca atcccgatga tcctgacagc aaagtagcaa tgactgttat atcaggcggg
      541 gttatccggg ataatattct ggttggcaaa acggggaagg ttgcattgtg tgatgccttt
      601 gcagcaatcc cgcttggcat tggtatggat gacaccatgg gctatccttt gatcacctgc
      661 tatctgtatg cctctgaggt taaaaaggcg ctggaaattt taaccagtat ttacccgctc
      721 aagggcagtg attattttat tcatatctcc ggagtaaagt ttacctataa tcccaaccgt
      781 atgctctttg acagggtaac ggaaatctgg attggcgatg aacatgccgg ttataagctt
      841 ctggattatt caagcaacaa taaaaaattg tatagagtat cagcagatat ttacaatgct
      901 acgtttttaa agattatagg taattttacg tggcacatac tggacattgt gcctaaatgg
      961 cgcgatggaa aacctataga aagcttaaag gatgctcgcg tggatgcaga caaaaagaag
     1021 gtgggtattc aggagttgaa ggaatggcag ggtgttattg catatataca aagtttcaag
     1081 gatgaagatg gcgacggcat acccgatata ccggcaatgt acaaggacaa gctcggcagg
     1141 atagtggttg aggcaagcct taatccgtat agcatgctca aacgtggcac aacggttaca
     1201 tggattgggt ttgtggtatt tctttttatt ctggcgatag ttggtggcat tgtgtatgca
     1261 gtggtaaaga ggatgcgaag atagtagatt cgtctgaata ccgctgattc cagtgtgatg
     1321 cttttatggt tttggttgaa agtacaataa taatctaccc tgctatgaat cctggtggtg
     1381 ttttcaatgc atgcgcatta caagaaaaag gtgcagatga gattatgtct ttagaaaaag
     1441 aggcttataa cgatgctatc atcaaaaaat tcagaaacaa tattatccat gcgcagaaaa
     1501 gttttaaaag aacagcacta taaaaaggca ctggataaaa ccaatagagc agttgcctat
     1561 ctgtatggct gtggtgcaca ggctgtttat atatttggtt cattactaaa taacaccatg
     1621 tttgatgaaa tgtccgatgt tgatatatat gtggaaggat tgccacggga aaagaaaaaa
     1681 ggcttgttca gcgcactgga tgaactattt ggtgaaataa aatttgatct gttttttgat
     1741 gatgatgtgc tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncataatg
     1861 atattgatgt tttttgtgca tttttatcca gcaaacaaca gtaaaaaata aaaaaaatta
     1921 aaaaaatttt taaaatttcc cccgcaaaag cgtattaata cataaacata ctgaggaggg
     1981 tactatgctt tcacgggcta cttcgctggt tatccatgga attgaagcac atctcattga
     2041 tgttgaggtt gatattgtaa aagggcttcc taaccttacc atcgttgggc tgcctgattc
     2101 cattatccgt gaatcaaagg acagaatccg ttcggcaatt gaaaattcag gatttgaatt
     2161 tccgccaaaa aatttcattg ttaatttagc acctgccggt taccgcaaag aaggagcaaa
     2221 tcttgatttg ccaattgcgg tttccattct ggtagctaca gggcaggttg agagtacaca
     2281 acttaccata cccatggttg gggagctttc gcttgatggc agggtgcgac ccattaaagg
     2341 tgttattgca atggcaattg cactgtacca tgctggtatc aaagagatta ttgttccgta
     2401 tgaaaatcgc aatgaggcaa cggctataga agggattaag gtgtaccctg taaaggatat
     2461 tgcccaggca atagaagcat gcaacggtac catgcagcca tacacagaac atgtcagtgg
     2521 cgatagctca tggaatggct accttgacat ggcaagcgtc atgggacagg aaacagctaa
     2581 acgtgcactg gaaatagctg ctgcaggacg gcacaatatt ttgatgtatg gaccgccagg
     2641 ctcagggaaa accatgcttg ctcggtgcgt tccaggcatt ttaccgccgc ttaccaaaga
     2701 gcaggcaatt atgaccacca tgatacattc ggttggtggt gtgcttactc atgggcaggg
     2761 attggtttat atgccgccgt tcagagctcc gcaccatacc agctctgacg cagcgctggt
     2821 tggcggagga aaaataccca atgttggcga ggtatcatta gctcataatg gcgtactgtt
     2881 tttagatgag tttgtcgaat tccgcaacga tgtgatacag gcgctgcgtc agccacttga
     2941 ggattgccag attaccatat cgcgtgcgct gggaaccatg acattccctg ccgattttat
     3001 gctggttgct tccagcaatc cgtgccagtg cggctacctc tttgaccctg aaataccgtg
     3061 caagtgtaca gcaggtaaaa tacgcaatta cttttcaaag attgccggcc ccatacttga
     3121 ccgcattgat atcgaggtgc ttgttggcag ggttccttat aaggatttac tgggcaaaag
     3181 caatgctgaa agctcggcaa gggtaaggga acgggtggta aaagcacgtg aaaaacaacg
     3241 gcagcgtttt tccggaagaa caaccacctg caatgcacgt atgacaagcg atgaggtcaa
     3301 agaattttgt gccatcaatg gcgagtgtga atcaattctg gaactggcaa taaagcgcat
     3361 gaatctatcc gctcgcagtt tttttagaat attgaaggtt gcacgaacca taaccgatct
     3421 tgatgatact gaatccattg aaaaacggca tatactggaa gcattgtcat ataaaaatct
     3481 acagcggtac tatgaaattt aatatatgtg ttgtacactg tatttttcta ttgacgaaag
     3541 tgcatgaaca taaaaaagct taatgtatag tattattcaa gggaggcttt ctatctggat
     3601 tgtttatgct ttgttatgtg cattgttcct tgctattggt gatgcgctgg ttaaaaacgc
     3661 accagcaatg catcctgtaa ccattgctgc cggacgtgtc ttcttttcat tgccggtgtt
     3721 atgggcgata gctcttataa acggtattcc tgctatcaat agtgatatta tctattcatt
     3781 gttgctttcg ccgccgttag aggcgattgc gcttgtttta tatatgcgtg cattgcagat
     3841 atcacccatg tacaggacac tcccgttttt gtcatttacc ccgcttctac ttattataac
     3901 ctcatgggtg ctgttacatg aacgggcatc cctgtcaggg cttattggta ttgtttgtgt
     3961 ggttttagga acgtatagcc tttattttca gcgtggtcag agtttgctgg tgccattgag
     4021 atccgttttt catgacaggg gttcattaat gatgctcatt gtggcggttt tgtatagcat
     4081 caccgcaaat tttggcaaga tgggggtagt ctactcaagc cccgcttttt ttggcgctgt
     4141 gtattttacc atacttttta ttattcttgg tatatggtgt gcctatgctg attctatcaa
     4201 aaatattttt accaaaaaca ttgttgccat tggaataacg caggggatta tgataacatt
     4261 ccacatgctt gctgtggcga tagctccggt aagctacatg gttgctgtca agcgttccag
     4321 catgctgatt ggcattatat ttggttatgt cttctttggt gaaaagaatc tattacagca
     4381 tggtatcgca gcgttgatta tactaacggg cattggaatt atcagcatcg cgggctgatg
     4441 ttgtaaaaat gatacagtga aattaatatt caacaggaag aggcatgaga ttgttaaata
     4501 atgtatacaa attattaaat ttgattggtt tacagtaact atcgccccta taaagcatga
     4561 aaaaagtgca tggtgtacat gatagtatga agaaaaacga taaaaattca taaaaaaata
     4621 taaaaaaatg catgagaaaa ggtgcacaca ataaaaatac tatgaataag tgaaaagttg
     4681 gcatgatagt tgcttatatt atggcatata ctaagtgaat atactatgaa tgtgtataga
     4741 taaggaggac tcaatggaaa atacagtttg ttcatcatgc attaacagaa atatatgcag
     4801 taaagaaagt ctggctcatt gtgaacacta tgatgttgta ttgacaaggg ctgaggtagt
     4861 tgatcttttg aaaaacactc gcgaacaaga ccctatcaaa tttcaatcca tattgaatgg
     4921 tatcaaaaag acctaccata ttaatttagc gtactaatca caatactata gaggggcggg
     4981 ttaccatccg ccctatttca ttacttttgc tctccccatt taaaatgatg aaatagtaat
     5041 tgtatcgtgt tatgccttga tgcacctaac aaagtgattt ctgttttttt gtaaaaggct
     5101 tactggtgca tgtaaacaat cggcatctcc ccggtagcat cgtggataaa atacacatcc
     5161 tgacggtggt ttggttataa tagggacagt accagggatt gttgccagcc gctttgattt
     5221 tttgtgcagt gaaggtatac agctcataag cccctgtgta taggggtgta aaaaattatt
     5281 aaatatttct ctggttggtg ctatctctac aatttccccg gcatacatta tggcaatgat
     5341 atcggcaacg tttgcaacaa tccctaaatc gtgtgtgatg agcagcattg acatatgctg
     5401 gcttttttgc agtgtcagca gcaggtcaag tatctgtgac tgaatggtaa catcaagcgc
     5461 tgttgttggt tcatcggcaa ttaaaagttc aggctttgcc gcaagcgcca tggcaatcat
     5521 tgcacgctgt cgcaaaccac ctgatagctc gtgaggatac gagtaatatc ggctggaagg
     5581 gctgggaatt cctgcttttc ccagtaatgc aatggtgtaa tcttttgctt cagctttctg
     5641 taaatgtaaa tggagctcaa ccgcttcctg gacctgatat ccaatagtaa atacaggatt
     5701 aagcgatgtc attggttcct gaaatatcat tgaaatgttt ttgccgcgta tggactgcaa
     5761 tgttgcatcc gggcattgca aaaggttcag ggtattgtac atgattgcac ctttttcaat
     5821 gacaccgggc tttggaacta actgcaacaa tgaatatgca gttaccgatt ttccgcatcc
     5881 tgattcgcca acaagcccta atgtttttgc tctgggtatt tgcagggaga gatcacgaac
     5941 ggcatagttt tttccgtaca tgctggtaaa tgcaatggta aggttttcta tagtaactat
     6001 cgcttgcatt atactatatt tctgcttcgt tttattaatt caaatccctg actctgaagc
     6061 atggcataca gttgcggtaa atcagcgttg tctcttttat atttatagat agttgcatct
     6121 ttataggcaa gaatttctgc tgttgcaaca tcaaccacct gtaacgtgcc gggtgcaacc
     6181 tctcgtgcgc gcttttcatt gatttgcgta aaggcaactt ttgtaatatc gttggtacgt
     6241 aatgtgttta taaactcgtg gatggaatca aacaatattt cgcgctgcat aacccacctt
     6301 actgttacaa atatcgtgga acaagtttaa tgtagttgcc gttaaagtca acgatataat
     6361 agcgtatagc ttttttatat ttattggtaa aagtattatc gcacggaaca ggaatatatt
     6421 gaatggtgtc gatagttacc ggtgatgaaa ttcctgtgac accaataaag gcaataacat
     6481 tgtactgttt cagcatggtg tgtaaatgct gataatcatt atgtatgaat gtttgtgagt
     6541 tgtttttatg tatggctggc agaaaatagc aaaaaacaac tttactggca tgctgatttt
     6601 gttttacatc atcatgaagc cactgaagct cctgttgagg aagtttaagc acattgttgt
     6661 cctcactgta cagtacaata aagtgatggc tgccatagtt aaatgaatac catgcctgct
     6721 tttgtgtaaa gcgtgtgtac aaccgggtgg agttattgta agtatcctgg ggtccacagg
     6781 cggtaaaaag tatactgttg agtttactgc aatagcggta aaactgttta tattgtgttt
     6841 ctatatcttt ttcatgtatt cccatagcga tggtaccgcc gtgtacaatg tttccggagt
     6901 gtatggtaaa taccggattt tcgttattga tggattgaat gatggtagca agttgtgcat
     6961 cataggctgc atagggtgaa ccgggcgaag tattgttaat gatgacaaac cgtataggtt
     7021 gcgtggttat atttttttgc acactgcatg ctgttactaa taaaagcaac aatgtacata
     7081 agcaatttaa tgggtatagt aatcgtttca tgcttaccat tgttggttac catatatttt
     7141 ttacaagttt ttattggtat tgccattaaa aatgtattaa caatatactc gatagggcac
     7201 acaatcgagt tgctgttgcc atgcgataag ggtacatcgt catgcaaatc gaggcaacca
     7261 gtataaaaaa tcgcatggca ccataccagg tataaaagaa tgtattaacg gtaatgaatt
     7321 tttttacatc ctgagttctc ttgacaagtt taaaaacaat gtgattatac gaccttattt
     7381 ttacacgcct tgagctttaa caccagggac aatcccatag tggtgattct atcgtgtcaa
     7441 tgaattattt tatgtttaca aatcccgtta gacttgacgt tgttatatga gcaggattat
     7501 attataagaa atttttgcta gtattaatac aatggttaat atcattaatc agtgagttat
     7561 ataacgctgt atgaatgcta aaatgggtag gtactgtcta tcttttgatg ccaacaattg
     7621 ttatcgggta tagagcattg taatggctaa atactgataa atgtgcaaaa tttgtcccac
     7681 gttatatatt gattagacat ttaatagcat tgcctgcaga ctaaattttt acataaaaat
     7741 ttaaaaaaat gatatataac ttgacatata caaattagaa taccattgta ttataaggta
     7801 ttaccaatag tgtttacaaa caatttttat aggaggtatt tatggctgat agatggtata
     7861 taacacaaca aacatttccc gagttgctta acaggaatgt aataaaatat ggcaccagaa
     7921 gggcacaatg gtggaaaaga ggggatacta cacattcaat aaactatgct gagcttggat
     7981 ttattgttaa acagttaacc gccggactca tgagtttagg aatacagaaa ggtgatcgcg
     8041 ttgctatagc atcgcatact gcacctcagt ggatgtgggc agattactca atactatgtg
     8101 ctggcggtat cactgtatgt atctatccaa cgctttctgc aaaagagatg agctttatca
     8161 tcaatgattc tggatcaaaa attttatatg tacaggatga agaaattctt caaagagccc
     8221 tgagtgtagc tgatgaaatg ccaacactgc aaaaatgtat tattatgaaa gatgattata
     8281 agggttctga ctctcgtgtt cttactctgc ctcaggtaag agaattgggt atacagctat
     8341 tagcaaaaga taaattagcg tatgaaagta gatggcgttc agttgatata cttgatccca
     8401 tgactattgt gtatacatcg ggaactactg ggaggcagaa aggggtagtc catactcatt
     8461 tcagcattaa tgcagcatgt tgccgggatt tgcgtattgt cccggaatat cgagaggatg
     8521 atattatgct ttcatttttg cctcttgcac acacctatga acgtgaatgt ggtcatggca
     8581 ctgccatgat ggctgcggtt accattgcat actcttcacc aatgacattg attgaagatt
     8641 tgaaggtgtt taaacccacg gtttttatgt ctgtaccaag aatatatgaa cgtgttttta
     8701 tggctatgcg cgatatggca tctaaatctc ctgttactaa aaaaatcttt gagtttgcaa
     8761 taaaaactgg tttgcaactt actgaagcaa ggtctgatga aaatggattt gtagatatgt
     8821 ccgaaggtat taattttact gaaggcgtag gcaaatggct ggcattcaaa tataaactgg
     8881 tggataaaat tatttttagc aaagttagag aaacactggg tggacgtttc aggtttgctt
     8941 tttctgctgc cgggagttta tctgctgatt tatgcaaagt ttttatggca atgggagtta
     9001 gaatttatga aggatatgga gctactgaaa catgtaatac catcaatctt aatctgcccc
     9061 ataaggtttt gcccggttat gttgggcctg tatgtaatgg cgtagaagga agaatagcaa
     9121 gcgatggtga atggcaggta cggggtaaca atatatttac tcattactgg aataatccag
     9181 aagcaacaaa tgaaactttt actgaagatg gattttataa aacaggtgat atcgtacaga
     9241 tgggacctga tggatatatc aaaattgtag acagaaagaa aggtattatg gtccttgata
     9301 ccgggaaaaa tgttccttcg gcaaagattg aaagcttatt ttcagtatct cgatatgtgg
     9361 atattgtatt ccctatagct gacgataaac cgtatgttgg cgcacttgtt gttccaaaat
     9421 atgacgtttt cattgaactg tttaaaaagg aaggtatccc atacgaccaa tctaaattgg
     9481 tgttttttga agagggtgca acacgcacat gtgttgccgt tggagatgat tttattcaga
     9541 atgaactgtt gcgcaaactg gtagatgaag acatacagca agccaataaa gaattggaag
     9601 aatatgaaag aattaaaaaa tatgtaatag tgaacagaaa atttagtgaa tcaaacgggg
     9661 agatgacccc aacacttaag gttaagcgca atgtagttct gaaaaatttt gaaaaagaga
     9721 tacaggagct ttataaaaag taaatttatt gtttattata ccatagtact gatatatagg
     9781 cagctaatgg gatgttttaa ggctgcctat atatcacact atttcagtgc caatacctgc
     9841 atcagtaaag atttccagta atactgcatg ttccacccta ccatcaatga tatgcgtttt
     9901 gccaacgcct tctttgagcg caccaagaca acaattaact ttgggtatca tacctccgct
     9961 aattttgtta ctgataatga gattatcaac ttcatcctta cgcaatgttg aaataagttt
    10021 gccatcaatt tttacacctt cgacatctgt taaaaggatt aatttttctg cctttaaagc
    10081 ctctgccagc ttgccagcag ctatgtcagc attaatgtta taggttatac cattattatc
    10141 acagccaata ggggctatga cggggataaa tttttccctg tccaatgttt caatgataag
    10201 aggatttact tttgttattg ttccaacctg cccaatatcc ttcttttcac cattggcagt
    10261 gtgataaatt ttttcagcaa taaggagatt gccatctttt ccggataaac caacagctct
    10321 gccaccagcc agattaatac aattaactat ctctttatta acttttccaa ctaaaaccat
    10381 ttcaacaatt tccatggttg aagcatcggt aacacgcata cctcctacaa acgcgctctg
    10441 tactcctacc tgctccaaca gtgtgttaat ctgaggtccg ccgccatgaa ctataacagg
    10501 attaatgcca acatatttca aaagtaccat gtccagcgca aatgaacgct taccttcaag
    10561 ggtttccatg gcatgaccac catattttat aacaatggtt ttaccataaa actctttaat
    10621 ataggggaat gactcaataa gcgtattaac tttttcaatg tattgtttca ttctatataa
    10681 acctcttcct tatcagggat tatcaaagat tgtgggatat cgtctttttc aacagcaaac
    10741 agtggcgata gttctatttt tttaagcata gtggggatag caataccttt ttgtgttaac
    10801 catcgtttat gtaaatgtat cataagttcc tgtgccgatt caactgaatc aacgcctgtt
    10861 ttatttttaa ccggtgcaaa ctcttcctca cgttttgtct ccaggatgaa attatgctgt
    10921 gttaacggaa gtgcatcgaa gacaaatttt tcaaacttat aaccggtaat gtgttgaata
    10981 cctttatcag tatatttggg tattgatttt tttgctacat ggaatggcag cgttacattg
    11041 ctgccagtaa cagatgcaat gaactgtgta ttaaaaagat gtattgcaat gcttcctgct
    11101 gcataacgca gttgtccctg ctcatctttt tcatattgct tctccggcgg caggtccgaa
    11161 tattctacaa ctccaatcct gttatttgaa aattgtacaa acactccaat cttttcttca
    11221 ggatatgcct ttaccagagc tttacttgaa acttcagcac tatgcattct atggaatcca
    11281 ataaatacag gatcaataat gttaaccagt gggttgtcca cctgaaagta tgaaagtatt
    11341 tctatggtta atgcctgcaa tgtatcaagt gtgcccgact gatataacgc tgttaaactt
    11401 ccaccatgcc cgtcaggatt tctaaaaata gagcagggcg attccagtat aagctttcca
    11461 ttttcatcca gtgaaggaat catgttttgc ttaaagagga agactttttc tttgttgaga
    11521 ttaaaaaaat tgctttgttc aaaatatgct actgttgcat catgattggc ttctgaggtc
    11581 atgatgcacc atggtatggt aacattatac tttatttcat acgcccttat tttttctgca
    11641 tggatctgaa acaatgattt tttgcttaca ggtcctattg gaaacatccc ctttgggcct
    11701 tcaaatccca agcggcttcc ctgcccacca gctaccacaa atgcagccac cttgcctgcc
    11761 ttaatggttg caatcccaat ttgttttgct ttttcaaatt cttttttttc ttcatcggtt
    11821 tctggcaaag gcgtatacgg cgctggttta aaatccaatg gaggcaggtt tctttttgta
    11881 agttcaaata gttgttgcag ggtttcaaaa tgtatggtcg atagttcctc taaaaggtgt
    11941 tttttctgat caggatttaa tgtatcccag taagcaaata catgttcatt cccggaatca
    12001 tatacattgt taataacggt ttcatagcca ggatagtgta tcttcataga gttccccttg
    12061 gtcaatgcaa tgctttccta taatctggag catggagtaa atttgtatgc atacagtgaa
    12121 atgcttttta acaactattc tgcatatcat gaattatcct gcaagatatt attaaaataa
    12181 aaccaatcag gataaaaacc agtgtatcac aaaaattatt accaatcaaa tgattaagga
    12241 tatcggctca tatccagaaa cacatgctgc tgcgttttgg gtgcatcaca attcgcccct
    12301 gcataatgtc atcataatga atacaaagat atattccatc acaatttaaa aacgactatt
    12361 tttttatttc aacagaaaat taataaaggc agtataaaaa taaaatgata tgtctacaaa
    12421 caatgtctgc ttttataaat gaaatgattt aaccaccagg caggtaaatc ccttaaaagt
    12481 atgctcgtca tcagccatat tattggtatt atagtcagat ttgatatatt gatagcggta
    12541 tcctaatgat gttgttaaac caatggatgg aaagtagtag gctaactgcc cggcaagatt
    12601 ataaccatat tcattataat ttgtatcatt agaattatcg ttgtgattgc catagagata
    12661 taaaccggat gcattgccta aaaaatagaa attatagacg atggggatgg taaagccaat
    12721 acccatgccc ggacctgctc catggtgctt accgttttca aaggtaaatc ctaaatattt
    12781 agctccagca aatagtttaa tatagcggtt taattgataa ttgagcgcta aatcagaatc
    12841 gtaacgcctg agttttgaat cttctgaccc ttcatcaggc atttcatact ttttgggagt
    12901 aaagagggct acaagagtta cacttaaagt ttctgaaaat tgatacgaaa gtgaagggcc
    12961 aatacataaa acagggtcca tggtcttttt gggattgcta ctgttgtcca tctcccaatc
    13021 ggcataccat gctgaaatgc caatacctaa atcatcagca tatactggca aaaccataca
    13081 catgcttatt atcactatac caatacagta tacatacttc atacattatc ctccgaaaat
    13141 ggaattatta tcctcactct ctgctccacc tcctttataa ggcgagggga gttttcgcac
    13201 cattgtcatt atgagggagc gttagtgacc gaagaatcta tgcttttaac gacgagattc
    13261 ttcactttgt tcagaatgac atgtcacagc aagattcttt gcctaacgct caggataaca
    13321 ggtgctatcg cttttgaata acagtttgct gatttctctt acctatcatc atgctaaacg
    13381 tatttcagca gcaattgttt gcaattgcac tatgtagagg agataatggc aagaattttt
    13441 taattatatg ataactatcg ctaaaaaata aggatttttg atcgtttaca atctattcac
    13501 aaaatcctcg atcctgtcta ttgcctcctt cagttgctca atagatgtgg cgtatgaaca
    13561 tcgtatgtga ttgtcatacc atgacccaaa cgctgtgccg ggaactactg caaccttgtg
    13621 gctttttaaa agtcgtacag caaattcacg cgaggataac cctgtctttg ctatgctggg
    13681 aaaaacataa aatgcacctc ggggaggata acatggcagt ccgatttctt gtaaccgtga
    13741 tgatataaaa ttacgccgtt taacatagtg cgaacgcatt gttgcaacgt cacgctgtcc
    13801 atgggtaatt gcttcaatag cagcatactg tgcaattgat ggagcgcaca atgcagtata
    13861 ctgatggatt ttattcattg cagaaataat actttccgag ccacatgcat accccagtcg
    13921 ccagcctgtc atggcatagg cttttgaaaa tccattgagg taaatggtgc gctcaggcat
    13981 gcccggtatt gaaataattg aaaaatgatt tccattatag gtaatttcat cataaatctc
    14041 gtcagcaatg acaatgagat tatggtgtat ggcaattgaa gctattgctt ccaatgtttg
    14101 tttgtcaatg gaagcaccag ttgggttagc aggataatta agcatgatag cttttgtttt
    14161 atgggtgata gttttttgta attggtcaat atcaatagca aatccgttgt caaaatgagt
    14221 tggtattgca acagctttcc catacgctaa tttgatggtt gcagaatagg aaacatagca
    14281 tggctcaaag tagatgacct catcaccggg attgatgata ctgcgcaaag cgatatcaag
    14341 cccttcgctg acaccattag ttacaagaat ctgagtttcg ggatcatata gtacattgtg
    14401 ttttttgctg atgatagctg ctatgtgttg ccgcagctct tgaagtccct tatttgaggt
    14461 atagtgagta ttgccatctt taatagcata aatacctgca tcagatatgc gccatggagt
    14521 aacaaagtcg ggttcgccaa ctcccagtga tatacagtca ggggtattat atacaatgtc
    14581 aaaaaattca cggatacctg atggcggcag ggttataaca ttgtccgata aaagattatc
    14641 aacattgttt gatgtaacaa catgcagctt catagtcttc tccaatcgta agtacaaaat
    14701 ataatataca atattgtatt cattttaaca ataaaatttt atagtttatc aatcctgtca
    14761 atctcccttg tttcagtggg ttgtgtcggg attggtgatg ttttgtacag cagtacataa
    14821 ataatcaaag ccgccagtat tatgcaaact atcgacacaa ttcgtagcct tagtaacaaa
    14881 aatcctgcgc atgcaattaa tagaccagca atgataacaa acattgtagt gttgttaaga
    14941 atatatgata caatttccat aactctactc ctgcatggtt gccatgttca tacagttatg
    15001 cctgtcattg ttaagaaaag gtgctgcatc actgtatgaa taactcaata aatcaatata
    15061 ccggtttgta atcaaagcgg tgtattgcgg tgatatatcg tatggtatgt gttttatagc
    15121 gcattacaat agaatgagtt tttgctcccc ctgccataaa tctgaccccg ggcaacagtt
    15181 caccatctga gattcccgtt gccgagaaca taatattttc gcctttagcc agatctgaga
    15241 gggtataaat tttatcaata tcggtaatcc ccatgttgcg caccagttct ttatcctttt
    15301 cgtcacggta aataaaacgc gcctgcatat cgccgtccag acatttaagc gctgctgccg
    15361 ataaaatacc ttcacgggca ccgccaactc ccatgagcat atcaatggga ctgtcatcaa
    15421 gtgcagtagc aacagcaccg gaaatatcac catccttaat taatttgata cgagcgccta
    15481 atgcccgtat acggctaacc aattcttcgt gacgtgctct gtcaagaatg cagaccgtta
    15541 tatcgtcgat atattttccc ttggcgcgtg caacacgttt aatattaacc tctggtgatt
    15601 gtgttatatc aataacacct cgtgcatctt taccaactgc tatcttttcc atatatatgg
    15661 gcggggcatt gagcattgag ccatattcag caagtgcaat aacggagatg gtgttatagc
    15721 caccctgagc taaggttgca tgcccatcta ttggcgtaag agcgatgtca acttcgggat
    15781 gctgagcatt accaattatt ttgccagtac aaagtgatga atcaggatta tcaatgccac
    15841 agataatatg gcccttaaat gaaaccgttg aaaaaacctt tgacattgct tccacagcat
    15901 ttttgccagc aagatcttca ttgccacggc catagtaacg cgctgcccag agtgctgcag
    15961 cttcggtaac cctgacaacc tcaagtgcta aattgcgatc catacaactt cccttaataa
    16021 ttctaatttt tccttctgag tatggctacc caatggattg cttttgcagg gagtatgaaa
    16081 atccctccag cttccaaata aaacagttta acacatacta ttgttataaa aattgtcaaa
    16141 ttaatatttt ttaaaaatga caggcaacta tcaataaagt ggaaatatca gtaatttttt
    16201 ttcaacaatg gaaaacatga ttttgcagta tgcctgtaat agattatgca tgccttgatt
    16261 taagaaacca catggaaaaa aaatagaaaa tagcggtaaa gaatacttta caaatgtata
    16321 tgtgcagtat aaggcatata cagcagggta agggtttatt gataaacatg ttacaggtaa
    16381 aacaaatttt taaaatagct ttgccagtga agctatggtt gcctgttttt gttgtatgta
    16441 tgttctggta tacggggaaa tatgtaaata ttttttgttc acaacatcat ggtatagctc
    16501 ttgcaatgtg gtaaggtatc gccccgcctt tggattaatg gtaacaacag ttgcataggg
    16561 gataataaat aatcggttgt agatagttga tgcaacataa aatccgttgg gtgaaataat
    16621 tgcctcatca ctttccactg ttgttgtaaa cagtctcccg ttaatactgt cgtacatcat
    16681 aataaatgca tttttaagaa agattgcatg atttcggctg acctgtaaac aggggttaag
    16741 tgattgctgc atgagcgttt catgcttttg cgtttttact gaatagcgat gtaccgtata
    16801 atttccggga tatccgcctg tataatagat ggcaaaacta tcgctgaaga cctccgccgg
    16861 ggtaacagca ttgcaaatct tcttccatag cttttgtttt atatagacag tgtagttgcc
    16921 tgatgaaccg cttacaaaca gttgattgct gttaaaaatg atagtacaga tttcatccca
    16981 ctgcggtgca tcgctgctgc aaagaaaagc tttttccatg gaactatcgc accggtatgc
    17041 aacaatgcag ttgttctttt tgtaataaaa aatgttgccg tcatctgaaa atgaaaagtc
    17101 gttgcctgct gataaggttt gtagcagttt gacggtaccg ccgatacaat caatgatgaa
    17161 aagtgcaggt tgaggtatcc tttgattggt aaagtattta acggctatgt agcggccgtt
    17221 ggatgatgtt cgcgctgaga taaccgttcc tttgaatgtg tacagtggat tttttttacg
    17281 tgttgttatg gcgcatacaa caatagtgtt gtttatacct gattccttaa tataaacaat
    17341 ttctttaccg ctgcaccatg cactgtaaac aatattagag tcaataaatt gagcacccat
    17401 ctgggcatag ttgttaagtc gtgcaaaaaa aagcttttga tattcagatg aatttttatc
    17461 catatataca gcatcaaggg gaatgccctt tgcaaaagct gtggtcgtta aacaaagtat
    17521 ggcaatagcg ctaaagatat agtgcattga ttattctttt acatcctgag gttttttatc
    17581 atccattgtt ttagattgtg attcctctat cggttgtttt tccttctcca tgggtggaat
    17641 aacaaaccgc tggccgggaa atatcaaatc aggatctttt atctgatctt tgtttgcaat
    17701 ataaatgagc ggccataacc gtgcattgtt atatactttc atagcaatcc gccataaaca
    17761 atcgcgtgct tttggattgt attgcacaat gtagtctttt tgttgttgcg taacttcttt
    17821 agtagtagta gcagttgttg tttcctgttt ggtttcttca ccttcctcct gtattgcaat
    17881 ggcttcttct ttcttttcca tggcaatgag catggtatta agaatatttg ttgcttgctg
    17941 cgaggtgtca tacgattcag ggtattgctc tgctttgaat ttatcctcgc ttgttttaat
    18001 gagcccttct gcctgggtaa gctgttcaga aaagtcctgt gctgtttcgc gctgacttac
    18061 ttcctgatat aaactttttg ctgcttctaa tgattcaagt gcattgcctt tatcaatggc
    18121 aactttagca gcaagcagtg attcctttgc ctgttgcatt gcatcaatag atgcagtgat
    18181 attttgttca tctaattttg tgttagcctc acttattttt gctgatgcat cctgtaactg
    18241 ttgtttggca aattcatccc cgcggttatt tgatagctca tctctttctt tttcaagagc
    18301 aacaagctgt tccttaagct gaggtatcat ggcaatggat ttaacctttg catcatttgc
    18361 gctggttatt gcttcacggg atttttgtat ggactcgcga tagtttgttt tattatgcaa
    18421 atcagatgac tccgttgcaa gcgccacagc ctgagcatat tcggctggtg caaattccct
    18481 ggcattgagc atctctattt cgctgatgct gttttttgcc tgggttacca tatcgctgga
    18541 atataagggc aatgattttt ctattgcctt ctgtgactgt tcatatgatt ccactgattt
    18601 tttctgagca tcctttgtct tgtcctcttt cagcagggta tgggtatcgt acagatactg
    18661 cttcgctttt tccaattcat cgggtgcgta ctgtggtgct tttacctctt cggcttcggt
    18721 gatgccatag cgcgcttttg acatatcgcg gataggtaaa tctgatgcgc atcccatagc
    18781 aaacgcaatg gatagtacaa ttatataata cataatgtgt ttcatggcga ctatctccct
    18841 ttactttttt tacaagtact atgattatag tatataggca aaaatttcaa gatatatttt
    18901 ttaaaaaacc tcgtgggcga cagttggcgg gaattttttg ttgacagtaa aataaatatt
    18961 tttttcataa atagtgttga cataccttga acaagcagta tcattacgca aattttaatt
    19021 gataaggtaa ttattacctg tttcgcaaac ctttgcgtac aggtggtaca caatgattga
    19081 atctgaggta ttggtactta atgcaggatt tattcctatc cgaataacaa cagtaaaaga
    19141 ggcaatatgc ctgttgctct ccgataaggc tgtgcctgtt gttgaagaaa atacttttgt
    19201 ccgttcccca tcaattgcca tcaaggtgcc atcggttatt gcaatcctga attatcatca
    19261 gctgccccgt cgtaaggtgg tattttcaaa attaaatgtt atataccgcg atgatatgca
    19321 gtgccagtac tgtggcaggc gctttcagat gaaacaactc accgttgatc atgttattcc
    19381 acggagccgc tgggagatag ttaccggcaa atcatttaag gaaggatttt caagctggca
    19441 caatttagta tgtgcctgta aatggtgcaa caaccagaag ggcagcaggc tgatgcatga
    19501 gattggctgg agcctgctca gggaaccgtt tgaacctgaa tacatcccct atattgttgt
    19561 aactgctgaa aaggcaaaga agaaggggtg gctccccttc tgcggattca atgttaagat
    19621 tgttgagatg atcccctgat atgttactgc tgtgcctgca ttggcatgca aggatttacc
    19681 agtaactatc gccacacatt tatttgcctt ttgtgtaatt aagcaaacca cccgctatga
    19741 taatggcgcg ctgacgctgt gaagcaagta acttgcaggt aatagccctg ccgttgattg
    19801 tcacaaccat ggtatcgttt tgtacctgtt ttacctgatt gatacaatcg tccatgacaa
    19861 cctcattgcc ctgctgtatg ctgtcgtaat cagattcgtg aacaaagaca agcggcagta
    19921 tgccaaagtt aataaggttt gcggtatgga tacgctcaaa tgattttgca atgaccatcc
    19981 tgattcccag atatgccgga caaatagcgg catgttcacg gcttgaaccc tgtccatagc
    20041 tgtgccctgc aacaataata gtagcaagcc ccttatcctt gttttgcagc gctcggtgtg
    20101 caaatgtgct gtcaacgtgt tcaaatacat actttgcata ttcggggata ttggagcgat
    20161 actttaagcg ctgacctgcc ggcatgatgt ggtcggtggt aatgttatcg ccaagtttta
    20221 tgccaacaac ccctttgacg gcatcaggaa gcggcgtggt ttttggcggc tgtccaatat
    20281 tgggtccgcg gaatattggc tcattgcttt gaaagggcgg tataatcatt gcatcgtcaa
    20341 tgacaaatga tgcaggcagt gcaacctgtg gataacgttc agcaggctgg tcaagcggat
    20401 tttgcagtgt gccgctcaat gctgacagcg ctgctgtttc cgggcttacc agatatacca
    20461 tggcgtcctt tgtccctgag cgcccttcaa aattgcggtt attggtacgc agcgacaccc
    20521 cgccggagat gggggcacca ccgttgccaa tgcaaaaacc gcaggcactt tccataatgc
    20581 gtacaccgct tgcaataata tcagctaaat agccttctgt ggcaagcatc tgcagcacct
    20641 gttttgaacc gggcgctaat atgcagctta cattgggatg aacctttttg cctttcagta
    20701 ttgttgcaac agtcactaaa tctttatacg atgagtttgt gcagcttcca atggcaacct
    20761 gatcaacctt catgcctttg atagcagcaa caggtaccac attgtcggga gagtgcgggc
    20821 atgcaatcat tggttcaacg gtggacaaat caagctcaat aaccttatgg tatgtagcat
    20881 cgctgtcggg aagcagttca acataatcat cagcgcgccc ctgtgcctgt aaaaattgca
    20941 aagttatttt gtcagcaggg aagattgagg tggtaacgcc taattcggca cccatgttgg
    21001 taatggttgc gcgttcgggt accgatagcg ttgcagcgcc aggtccggta tattctatta
    21061 catagcctac atttcctctg gtggtaagca ttttcaagat ggtaaggata atgtctttag
    21121 cgcttaccca tgctcgcagc tttccggtta aatatacatt gataacctgt ggacaggtta
    21181 aataaaaaag tccaccaccc atggcaactg ccacatcaag cccgcctgca cccatagcaa
    21241 gcattcccat accgccagca gtaggtgtgt ggctatccga gccaagcagt gttttacccg
    21301 gtatagcaaa acgttccagg tgaacctgat gacatattcc attgcctgcc ttagaatagt
    21361 agatgccata gcgtgcgttc attgtttgta aatatttatg gtcgtcggcg ttttcaaaac
    21421 caacctgcaa tgtgttgtga tcaacatacg aaacggaaag ctgggttttt acccgatcaa
    21481 tccccattgc ttcaaactgc agatatgcca tggtgccggt agcatcctgc gttagcgtct
    21541 ggtcaatggt tatgccaatc tcatcaccgg catttagatt gccttcctgt atatgtgagc
    21601 gtagtatttt atagcaaacc gagtaaccca tggtaaaacc tcttatatag gaattaagta
    21661 ttcagtatca aagttagcgc atcagcaaag tttttcaggt ttgtggcgat agttaccctg
    21721 caacaatcaa tatcggtata ggcaggtgca gcggaaacaa aatccctgca acgtgtgaca
    21781 atatcattga tggcagcttc atcgtataaa agtgaatgca gtttgccacc gggataggca
    21841 agcataagcg atagttgctg atgataggtg gttggattta aaagaataca gttgcagttg
    21901 cacaacatcg cctcgtaaac ggttatacca aagtgtgtta tcacaacctt actgcgggca
    21961 atggagtggg caccatgctg caatgaagtg cagccagtat tgcttatgac cttgttgcca
    22021 tcagagcaca catacgtaaa cttattgccc attgcagcaa taaggtgctg gctatcaaag
    22081 gttgaggcat acagaaagca atcaatatct ttttcaacgg ttgatggaag tgcctgcaag
    22141 gattcataaa agttatagcc gtataaaaac aaatcgttgc cggctgcaaa gctattgctg
    22201 gttgcagtgt gtagagtggg cagcaggtca attgccctga atgcaagcgg gcgccccttt
    22261 ccgcagtcat caatgacaat aacaggagct atcgcctgca attgttgtat ctcttcttct
    22321 gttgaatcgc gcatgtcacg tagtatgcag gtggtttgtt tgggaatgct ggccgccacc
    22381 atggattgca gcgaggatgg caccgtatat gctttgcttg catgcacaag tgttacgctc
    22441 cagcggcgcc tggttgctaa aaaccatgcc agcaagagca tccgctgaag atgtccctgc
    22501 ccaatatgag cgcccgaatc ggttacaatt gttatgtgca tactattcat gcgaggtcat
    22561 gggtttttgc tgtactgctg catttatcga cacaagtgcg ggattttttt ctaagtattt
    22621 gatgacatca tcaagggaaa atactgtgcc cgtatacaga gcatcataca gatgctttgc
    22681 aagtgtataa tcgtcctggg tatccacggt taagcgtagc ttgctgaagg gattatgcag
    22741 tggtacatca aaatagttaa tggcaaatat gtccgggttt tcttttatat agggtgtaac
    22801 atgctcaaag tggtgtggtt gtgtgctttg ggtgtatgcg cgctccaacg cctgtgcttt
    22861 gattacctca acaccggtgc ccagtggaag gttttttgcc gcacacaggt ctgtgttgag
    22921 ctttatagca gtgtcaatga ccattgatgc ataggctgca tcggtaaaag gattgtcggc
    22981 ggtgatgcgt acaatatagc tgccgccaaa acgcctgtat gccctataaa aacgttccag
    23041 aacattgtgc atggagccgc ggaatacaga gacattgcat tgctgtgcga tagttaccag
    23101 tgaatcattt tcactgtcat cagggattgc aagcacaacg gtgtttacag cgctgattgc
    23161 ccttgcacgc tcaataacat gcgcaagcag tggcttgccg gcaagtggca tcaccgactt
    23221 gtgaggtaga cgtgttgatt gcatgcgtgc ctgtataata gcgattaccg attgattgtt
    23281 tgcagtcatg gatattttat cctgattata atgctatacc cgcctgagga atacatggta
    23341 ctatacaaaa agcaggcagc agtattagta ctgtaatatc gtgatggatt gctttctatc
    23401 aagtataatt ttatacaatg ggaaaaaaat agtgcacatg ggtaggtgcc atgcaattga
    23461 attgtactgt gcatgcaata tgggcacagt aattattact tgacatagtt atagtaattg
    23521 tggtatctta ttgacaggtt gtataccatt aatcatcaaa aaatacaaaa ggatagttta
    23581 aatgattaac acatttgaga tatttcaaaa tctaaaggaa acctttggtg aaaaggaagc
    23641 tacagtatta accaatttac ttataaaaat gtacaatgan nnnnnnnnnn nnnnnnnnnn
    23701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23761 nnnnnnnnnn nnnnnnnnnc aaaagaggac tgaggagcgt gtagaggaac tggcacaggc
    23821 acaaaagagg actgaagaaa gcataaaaga acttacccgt gcacaacggg agaatgaaaa
    23881 aatcctgaaa aattttatgg tagcatttga tgatcttaaa aaacaggttg gtggactttc
    23941 tatggttgtg gggtatggaa ttgaagatga tttgatgcca ttgatgcctc agtttgtcca
    24001 gaatacttat aaggtaaaat catgcgaggt tgaacgaaaa tatattttat atgatgatgg
    24061 taattatgat gaggttaatc ttcatatacg tgcaaaaagg aatggcaaag atgtatatat
    24121 tataggtgaa tgcaaggccc aacccggtaa aaaagatata gatagatttg ataagatgtt
    24181 aaaaaggatt aaaaatcatt ataaatctga catttatgga tttattgtag gctataccta
    24241 tcatcctgaa atagaagaat actgtaaaaa acatgccatt gatattttta aaacgtatga
    24301 aataaagcgt ttggccgaag taaaaaagag aaataaaaaa cagtaaaaag aaagagcaaa
    24361 aagtataatc acacgttaag agcaaggtag tagccatgca catgacgctt taacaaacga
    24421 aagagaagaa aaatacataa tagtaatgtt atcataagca aattgtacaa aatgaagctg
    24481 atactgtata atgtgtaaca aaattaccta taaattttat gttgagtaat ctaatactat
    24541 aaacactaac tattatatag agggagggct ctatgatagc aacacaacat tttttacagc
    24601 gtgcgcataa cagaggtatt acaaatatgg agcttcaact ggtgcagatg tacgggtatc
    24661 aaaaaaacga ctgtattatc ctggatagaa agcaaaccag ggatatgctg gatgagctaa
    24721 acaagctgcg cagggtattg ataaatattt atgaaaaggg tggtatttca atggtggtgc
    24781 aggatgatag cttaataact acctatccta tcaataggcg cattaacaag aaaaaggcaa
    24841 aatatcatgt gtaatatttg atgaggcttg caataacaat gcactgttgc aagcctcttt
    24901 tttttaatta ttctgtatta ttgatgtgat gctgtaacta ctcagaaact gtgggaaaaa
    24961 tcaagcatgg gatgatagag gagttatata aatattaaaa tgttattgac taatactata
    25021 aaggtaatac catgaaaaaa cagcgcttat atattttaat aataatgatg ataaacaaca
    25081 tgattggcaa taaatcaaaa gaagaactat taaaagaaaa ttttgaatac atagacaagt
    25141 caataagaaa tgttaataat aaatataatt ataatcttac agatgaagat agggaagata
    25201 ttttacagca gatatgttta aagctgtatg aaacaggttt tgataatttt caatggcagt
    25261 gcaaggtaac aacatatatc tttaaaatag tagaaaatga aatacgatcc tatgtacgaa
    25321 aaagaaataa aataatagta tctacggatt taagaaagca tgatgaacaa tataataata
    25381 acattacaga agattttatc aataatatct cctatactat agattttgct cagcaactaa
    25441 ccaatgatga agtagctaaa ataattaagg ataccgttga tgcaatgcct gctttacaac
    25501 agcttatttt taaattacat tttgtagatg gaataaaaca ggaagatgtc gcaaagatgt
    25561 gcaaacggtc acaggctact atcgctgagc atataggaaa tatacggaaa agcattaaac
    25621 aggaagtgga aaaaaagttc aaaggattta taaaggagtt gcagtaacat gggacactta
    25681 ggcaatgatc tgcaatgtag cagccagatg gaagaattgg tactgggtgt tgctgatgga
    25741 acaattactg ataatgccat tattgatgag gtgctggagc atttagtcca ttgcaactat
    25801 tgctatgaac tatataaaga ttattttatt attgcacaac ccttactcac ggaacaacat
    25861 ggtgatgata cacttaccct tgatattgat tatgataatg gttttatacc gctgaatgta
    25921 aatgctcaat cctttgctgc ccaaaatgtt gttgctgttt tgtcaaaaca aaaggaaccg
    25981 gtaattgagt ttgaaatacc cttagcggat ggtgcgctga ctgtaaggat aatacatgtt
    26041 gatggtgttt ttgatattgt tgtttcaaat agtaacaata gtaatggcgc aaagatattt
    26101 ttattaactg atgaaaaatt tgaagtggca acattgtcaa ataacagagc aacatttacc
    26161 tttataaagc caggtattat tactttaata tacaatttta aaaaaattat aaccataaaa
    26221 ttaaactgta aatagaacat gtaataataa tttgttatac tttacgctgt tgtataacta
    26281 acagggtatg atagtatgag cataatttat attgatgaaa gcggagcatt tgaccagtat
    26341 caaaaaaagc gtaatgttat tggtggtata tggtgcaaga tgccagtacc acacaatgaa
    26401 gtaaaacagt tattttcaca atgtggacta tcaaaagttc atgcctgcga actggataac
    26461 gagacgcttt cattgcttgt tcaaaagctt ataccatttt gtgaaggtca ttccataaag
    26521 cccgttattt tgcttccacg aaggggtttt ttcattatta gcgatacagc cacctatatt
    26581 aatgtgctga gcgatggatt ggttgaattt ataaaaactt atgcaaaaga acttgatgcc
    26641 actattgaaa tcattattga acacagaaaa ggctttaaag tagatgttga tacaaaaagg
    26701 ctccatgagg caattgcaaa aggagtagcg cttagcaaag tacaaaacaa tataaactac
    26761 acaataacat ttagagggaa agataatatt tttttgcaac tggcagatgt ggtggttcac
    26821 agcttttacc gtcttgacca tgatggcagc tttttaaaag gtgaggtaaa agaagcattt
    26881 aaggaatgga tggaaccaaa cacagtatat atttatggca atgaatcgta tgtaacaaga
    26941 atttatgatt gcctgcgaaa gggcgattat ggtgaagcct tacagctcta tgcaaaccat
    27001 tacatgattc ctgaagtacg gcaacagaaa gattttataa taaaaaacat actgaaattg
    27061 agtcagccaa ccatggcggt agttcttaaa aacatactga cattatacta tgataaggta
    27121 aataccaacc gtttgttaaa tgaatatgag ccattggtta aactgtggat ttatacgata
    27181 ttgccggata ttgaaaaagc aatagctgat ggtaataaca ccagcacagc tgttgatgat
    27241 ttgtactggg catatggtga actgtatcaa attttgttaa ccatatacaa tcataaaggg
    27301 cagatagctg attttgaaac tacctataac gaagcacgga tactgttaaa aaaagcagta
    27361 tatgaccttg attcattatc cgtgcggata cgaatacagg tgttaaatgc tgtacactat
    27421 attaaccagt atcagtttga gcaggcgtat accagcatga aagtattagc taaaagggtg
    27481 gaagatgcgt ttgtcttttt atctgaagcg gataatgatg ttaaggtaaa gccgcggata
    27541 cttggcgaaa tatacggaac aatggtacag gcatgtatgt accatacgct tgttactaaa
    27601 aacaatttca atgaggcacg ggagctttca aataaggcac tacatttatt ttctcataca
    27661 gatgatatac agcggcagta tcagtatcgt gctcagattg aaacttacga tggcaattac
    27721 gaacaggcta tgcaatatat tgcaaaaggg gtgggatgca caacatacag cgacgatgat
    27781 ttaattgcgc aatgtttaaa aaataaatcc atgtttgggt tgcaacattt attgcgtatc
    27841 tggtactgca aacttatgaa tggttatgaa aatacttatt ataccgtttt gtcaaatata
    27901 aacaaaccag gaagtacaat agcaaaaaat tataaagaga taatggaaca caaggtttat
    27961 cctgttcata gtatataccg gtgtgttatt gttatatatg gtttattgca gtcaaaacat
    28021 tgggaagaac atattaataa actgttgccc agagcaaaag aattatttga tggacagcca
    28081 ttaacaatgc aaacattaca gttaggattg cttgctgatg ttttgtggat atttgcacac
    28141 catgatgcag aaaataaaga aatcatggat agatttaaca aacgatttaa tgatgtgatg
    28201 catgaggcat caaaatatgc aattggagat tatctgcaaa aggcatatca gcactatcag
    28261 caaacaccgg taaaaaagtg gaatacatta tggtgggttt tcccttttta aaaaaaagaa
    28321 cattgccagt aactatcgcc cataacatag gtatgattaa tttccataat aaacataatt
    28381 gtataatgca atatcatcgt tgccaaagtt tttttgtaat tcttgcagtg cgctggcata
    28441 ggcgttataa taggtagtgt tgctggtaat attttttaaa aaggttttta taaaaacaaa
    28501 gctgcgttgt gcttccagaa aaaagtttgt gccaatgtat gttgcaactc ccatttttag
    28561 cacggcatac gcaaacgaat tttttaaaaa taaactgccg cttgcagatt ggcaggcatt
    28621 aacaaccatg cattgtggtg ggcttgcatg aaaaatttca caatcatcaa tggaaaatat
    28681 acttccatcc ctgcatacaa gtccggtagt ttcaggtgtt gtgctgaata cagcatgccc
    28741 gtagtaaatg ataactgcgc aggggaaatg tttgcttatg gaaaccttta gattgcttgc
    28801 actggcagct tcaacagttg ttatattgta agcgttgtga gagcttgcaa gcattttaat
    28861 gttttgttca aatttgttaa aatcagggat attgttagaa gagccaaata tgatgataat
    28921 ttttttattg gaactataga cggaaggttc ggaaaattct ttatccctgt tgggaatcct
    28981 gacaatggga agctgtatgc catagtgtga tagtaccagc tcccatggga ttgatgcaac
    29041 ctcttcatcg gcaatgatgc gcatgttgct gataggacag ttggttttta aaaatgttgt
    29101 aagctcgcgc gaaaaaagaa ggctgcaaag tgttagaaca tgtgactgtg tatgtaagac
    29161 ggtatagttc ttgctatgac ttattgagta attgatatca tcgcggcatg atgatagcag
    29221 attgcttaat tgttcaatat cattgctatg tatttttttt gttccaagtt tgtacgagca
    29281 gtcattgaaa tagcattcat atcgcatggt attgttatta tcatcatagg caatgcgtaa
    29341 cacattgtcg tgtacagaaa attctataat tgttgaggct ttttgaatat agagtatttc
    29401 tgagccaggc tggggtatac ctgattctaa atactgttta tttgaatcaa tatatgctat
    29461 atccagtttc agtgatgatg caataatgcc aatttgtgtg gacattatct ttttacctga
    29521 ggttatatcg cataagacag gcggaaattt ttttgaaaat tcctttatgg tgcgctgcat
    29581 tatggtaata ttatcttgat gattggtggg gctaagctca acgggatgta gctctatgcc
    29641 acgggtagca caatggtgtt ttatttgttg aagaagctgt tccttttcag gtgtatacaa
    29701 taatacacaa tggtgaggaa gtatggcatt gagcataagc agcacagggt gtatattggg
    29761 cgaaagtatg gttattacag tgctgtaggg ttttgcatgc tcggatagta agtagtgtga
    29821 tagctggtta tgcaacgatt ggcttattac cggtaaaatt tcttctgtgt aaaacgaata
    29881 gcgctttttt tttgggaggt tgttccattg tttggttaat gtcattaatg attgcatgta
    29941 taaagaaaaa aataataata taccagtgtc aacaaaattt taacactcca tgctgatttg
    30001 tgcaaattta catttttagt acagtataaa aaaaattact gcctgacatt agcttttcaa
    30061 ggatctgttt gtattgcata atgcaactgc tttttggtaa tattttatat tatgcttttt
    30121 aattttatct tcatagcggc taataaaaga tgatagcctt gtaatattac taattttttt
    30181 ataattacca atatgctttt tattcataaa tgcatgacat agaatacata gtacctgtaa
    30241 attatcttga tgattattgg ctttattgcc gtcaatgtga tgcacttcaa taaagcgtct
    30301 atcagcaaaa ttactatcta tagtaatttt acaatgtgag cacgtataat tttttctttt
    30361 gcgtagttcg ttgcttatat taacccagtt gcttgtatag tttttgtaat cgcgttgaat
    30421 tggcaatggt aaatcatcct tgccaaatgt accaccaatt ttgttgttat taatataatt
    30481 attacaatat tcagataccg taataactct ctttatattc ttacttaaat ttaaacagtt
    30541 tttgcataat tttaattctt tttcaatgcc gtcaatatct ttcattttta caggataatt
    30601 attaaattca taatggccat caaaattatt catctccatt tgttcttcaa tggttttaca
    30661 tttataaata tgaaatttag ggacaatggt accaatatta tttgttttag cataatgaat
    30721 gtcgtaccct ttttttatat atatataccc tttaattttg tccttgtttc gttgtaagaa
    30781 aatcccatct tcattaaaac aaatgttttt tagaagttcg tcctgattca atgtcttttg
    30841 tattacaggc aaaggtgttt ttggtttttc cattttacca tgggttaatt gtaacactac
    30901 ctcttttaat tgtgaaaaat catagatgtc gttattgcta tgcatttaat tacccatggt
    30961 atccaatatg gttttaacaa ttgcatcgga ttttgttact attttaaatt caacccttcg
    31021 tgattgcata cgattttctt ttttccctga ttccagtaca aagttgccat cgctatcaag
    31081 aaccctgccc catgaaaacc ctgttgcggt taaccaaaat cgtactattt ttttatcgtc
    31141 ttcgtttaag gtataatagg gagatgaagg gctttcaaaa aagtattgca gtacgctttg
    31201 tgttcgctca taactaagtc taaggttata taaataatca ccccatgagt cagtatgtcc
    31261 ttcaattctt acttcagcaa tatgcttttt atatggcgct tttagcaaaa tagtaaaata
    31321 ccgtgtaaaa aaattatcaa gaatattttt aaattgtgga cggaggtcgc ttttgttgta
    31381 atcaaacaat accttttcgt ttttaaagcg gatggttaaa tcttcaccta ttatggcatc
    31441 ccatctttct tttttaaagt cattttcaaa ttctttttgt aattcgttaa acagtgccat
    31501 ctttacattt ttatattctt caagaatctg gttgcgctct ttttgctgct gttttagctg
    31561 taatatatag gcaacagaaa taaacataaa aataaccatt aaaccagtca tgatatcgga
    31621 cattgaaatc cagtaatgat ctgatttttt atgcatatat tactccgtat ataggttatt
    31681 tccttcagga tttaaaatgc tatcaagcct ttcggcatac tctgttacca tgctttgtaa
    31741 tactgcatca agattttcaa atgatttatt gagccgttca taaaaatatc tatcgctttc
    31801 ttcaaatttt ttcaacaatt ccatatttcc tttttcaata aagttgattg tttcattcat
    31861 cctttcttta atatctgccc aaaattgtcc acttgtatca cgaagatttt caatctcttt
    31921 aaggttatca atgagctttg caatactttg gcctaaatct ttttccatgt ccatccagtt
    31981 ttgtaaagta atggcgcttt tatccatttt ttcagttgcc tgtagtagat gttttgctga
    32041 atcaaccagc atggtgttct ttgtaagcac tgcttccaat tcttgaataa gtgcttttag
    32101 ctttccttcg ccgtcaatga gttctttggt gtattgtgat atcttttcaa gattaccaga
    32161 agtaatgccc agctcagatg atatggtttt aaactgattt ataagtgttt gcacctgttc
    32221 tttgttttca tgctgccatt gattaagacg ttccaccgat tgattaagtt cttcaaagtt
    32281 ttcttttacc agacgttgca acaattcatt caagcgctcg ttaaatccgc caattacatt
    32341 ttcaatggct ttaactaatg cctcggtatt gctcttttca agtaatttgc caaactcttc
    32401 aaatttactc cctataaatt ctcgtgattc cagtaaaaga tttttaaatg attgcacatg
    32461 gtcatttgta tttttgataa agtcagattg ttccatgcgc atcttactga tttgagttaa
    32521 aaggcttgtt tcagtatcag ctcccacatt tttatgtatg gattccagta tcttgttttg
    32581 ttcatcaaaa ttatcatgta acgaagtgcg caattttata agataagtgg ctatgctgtt
    32641 gtcgttatcc ccatggatgt ttgtaatgag ttcatccagt ttagtagtat ggtgttcttt
    32701 aaaatcagca aaaagcttaa gcaactcttt taaaaccgaa agctcatcat caacatacaa
    32761 ttgatttttt ctagtatgtt gaatattttc aatgaaacga gaactaatta aagaaaatat
    32821 aattcctaat atagaggtta aaaaagcagt ttttaaaccc ccaagcagat atggtatact
    32881 ggtggttata ttatttacat caaaattcat aagtgcaatg aaaattccaa caaaggtccc
    32941 cagaatacct aatgtggtaa agatgccggg gattgaatct aaaaatatat cattaatagt
    33001 ttgcggttta ttatatgcct gccatatata ataaccactg tacatggcaa aaaaaagtat
    33061 taccagtgtt gctataactg ttattgcaat cataataact cctgtatcta ttgtaacata
    33121 agcctttaat aaccagcaaa gaattagttg caaagtaact atcgaacaat aattagacat
    33181 gctgaggcat tattgtaatg gtaaatttta tttatactgt gcagtttttc tactattttt
    33241 tatgcaatgt gatgaaaaaa aatcctatat ttatggttga tttactatca aataggatac
    33301 aggtgcaaaa gtatcaggta atttttgtaa aaaaatggat tgttaaaaac tattgcatgc
    33361 attatattac atagtaacga tgaggttgag gtaagcagta gtggtacctg gacaatgact
    33421 atcgccatat aattaatggt ttgcttatta taaaaaaagc atactactat accatatatg
    33481 aggaggaaac tatgaaacgc tacacaacgc tcctgctcat ccttgctgta gctggcttac
    33541 tggcatgtgg ttctaactat gttcgcacga attcgtcagg tagttcatac aactatagct
    33601 acgatgaacg agactttaac aagtataagt ggtcagcatg gcagcgttca cagtgggtgc
    33661 agaaccacaa cggctggatt ttgaacgtta agaagtgtac ctaccacaac aggtggaatg
    33721 gccacacgtt tgccatgtgg tcgtttgatc ccaagtgcga atcgttttat accgcctatc
    33781 agataacccc cgagatgtgc catggggaaa acccgaaaaa gttcttatgg gaacgcgggt
    33841 ttagaaacat tatgtactat gagccagcta acgtggatat tgagtatttg aacccaaacg
    33901 ttgcaaactt tacacgtgag gaaaagtcat tcacaacagc tggtacagtc atagggccac
    33961 ggaaaattgg tgtcaataaa ggtaccgcaa tagccaatgc tcctgagccg ctaacacccc
    34021 aggaagcggc ggcaaaagag cgtgaagaga ggaaacgtat tatggccatg caagactacg
    34081 ataaaaaggc agcggaatta aacaactata tagtacagat caataaggca acccggttac
    34141 tcaatgccta ccttgcggca accaatcaaa cattgaccga agaagaagca gcatatcagg
    34201 catggctcgt aaacgccatg attgcgacat gtcgggcaga tgtctatgat aaaacgtcat
    34261 ggtataggta tgaggacggc actattgagc atctacaaaa ttactactgt ttgcataatc
    34321 tctattcaaa gtttgtattt cgattttcga ttgatctgga tgggaagaac acagaaccca
    34381 atcacttacg tcaattttgt gatgagtact ggaaaaagta tcatgaaagt gagcaagctg
    34441 caaaaaataa gtaggataca gcatcaatca tagacgaaac gaacttcgtt aacgacaggc
    34501 gtagcgccgt tggtggtggc gatttccatg acaacgtagt agggaacagc atcgaggtca
    34561 gtatactgat ttggccccag cgtcgctacg tagttctggt ccataagatt gaatgacgac
    34621 agcgaggtat agagtttata cgatatacca tcgagttgag gaacatgggg cgatagttat
    34681 tggtaaacaa atgtatccag cacaaacaag gcaatgctta taacaacatt ttttgtgcga
    34741 tagttacaga cctatgggat caaaagaagt cttatcgtgc acaatccaga acttatcaaa
    34801 tacaagctct caccctgtgt taacatagat gtcgcctaaa atatttagga gacggatatg
    34861 aaatacacaa gaagga
//
LOCUS       DAOOJH010000018        33352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1435_length_33352_cov_9.801303, whole genome shotgun sequence.
ACCESSION   DAOOJH010000018 DAOOJH010000000
VERSION     DAOOJH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 33352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33352
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1435_length_33352_cov_9.801303"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLJ75_04295"
     CDS             complement(<1..287)
                     /locus_tag="PLJ75_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04467.1"
                     /translation="MRYIRLSAIIVVIVGMVLVGFFPEKVQSRIVTNVQPDISAEQYS
                     ALVVEDFETQADWQLETIPKKNPDPKKDPVPVLELKYVDGGPADLLTEKWSP"
     gene            complement(328..1068)
                     /locus_tag="PLJ75_04300"
     CDS             complement(328..1068)
                     /locus_tag="PLJ75_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPD04468.1"
                     /translation="MRREVRSFILSLAIGGLMVAVGGGNIVVQAQEAQQAQQQKKQQT
                     GLVEDWMDDFEASEDWRAMATCPIGETKIRKIPGKPRPIDENGNEVNYPDTITDENGI
                     THENKYVLGVKTYFMDRGFDRVEVFPPNEYIVRGKAKEIKVWALGRKFRHTLFVKLRD
                     YKGAIHKIKIGRLDFWGWREMSVIVPGWLPQSPGYALLDKNLHFVSFYVESDIYEVPG
                     TFYFYLDNFRVITDLSEFTGDTSIRDNW"
     gene            1372..2235
                     /gene="metF"
                     /locus_tag="PLJ75_04305"
     CDS             1372..2235
                     /gene="metF"
                     /locus_tag="PLJ75_04305"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HPD04469.1"
                     /translation="MDIAVCLQHKKPLLSFEVFPPKTSSGEENLFKELSILVHYKPDF
                     ISVTYGAGGSTRDKTLEIAIRIKKSFSIEPLVHFTCVGQSKDDIVSYLHYVKTLGFSN
                     ILALRGDPPMGETHFKPHPGGFTHANELVSFIRSMNHFTIGVAGYPEGHIEAPDLDTD
                     IANLKKKVDAGADFIITQLFFNNDKFYTFLDKTIKAGITIPILPGIMPLTSIAQLTKI
                     TTMCNPDIPNSLLQELEKCSQPDDMCKVGIEFTIQQCQQLRSYGVPGFHFYPLNKSAA
                     IKLILDEFWHT"
     gene            2298..3188
                     /locus_tag="PLJ75_04310"
     CDS             2298..3188
                     /locus_tag="PLJ75_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04470.1"
                     /translation="MKSYPFYFYTGIMLIAFMQLCILFHVSHVTIWATPTLWTGLILL
                     TDGILAKSNRSLIKNRTIVKLSLLSFLCWWMFEWFNIFLSNWHYQGLIQSLPIRYFGY
                     IWSFSTIFPGVLLVYAIIATILPKLFFKPIHITSSFLVVSFIIGLFFIFIPIIPFSMH
                     YTNRAADPDLFFWLTWAANIHYSEFTAGFTWLGFIFLLEPVNYLLNRPSIYADLIVGK
                     YTRLVSLLAAGLLCGYLWEFWNYWAATKWIYTVPILPSLKLFEMPIPGYIGFLAFAWE
                     IYAITALVYPKALYTLEGER"
     gene            3193..4821
                     /locus_tag="PLJ75_04315"
     CDS             3193..4821
                     /locus_tag="PLJ75_04315"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HPD04471.1"
                     /translation="MEDIIVIGHKNPDTDSICAAYCYAQLKNSVDSTNRYRAYRCGNI
                     NRQTRFVFNQIGLQPPDLINNVYPKVNDIMTKNVITAYADDPLYSIIKHLEQDRIRII
                     PIVDSTNVFKGIVSFNEIAQFYFPVTSIAKPVYLFKPQNFNLTIPGRFIQQGKDKEFE
                     AAIVIGAMESTRSIEELHNVIPEKTVLLVGDRKEIIEFAIKNNFAALLITGVNDFTYD
                     TTHYKGCIYLSEIDTYNTLRMLTLSIPCHSIMQDADTISPDLPVEQAREQLLLNEYRG
                     LPVCDNTILKGIITRSDIIKHYAKKVILVDHNELSQAVDGAQTAQIIEIIDHHRLGSL
                     KTTTPIFVYSKPVGSTCTLVYELFKYNNVKPEPHIALLLCSGILSDTTMLLSPTTTET
                     DKKSLEELAAIAGINYNDYGQKLLSSTESLKTIDPIYAVSNDCKIYEEYGIAVAISQV
                     EVITLDGINEVKESLLSALVQFAQSRNVHWALLLVTDITTQNSILLSTDFKGEVHLNY
                     PPIEPHIYSLPGILSRKKQLLPEILRILEELSHK"
     gene            complement(4893..5744)
                     /locus_tag="PLJ75_04320"
     CDS             complement(4893..5744)
                     /locus_tag="PLJ75_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPD04472.1"
                     /translation="MIINHNLSAINSNKVLKFKHWDVNRSMEQLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLNQTATILQRIRVLAVQSSNGIY
                     TLEDRQLIQVEVSALVDEIDRIASQAEFNKFKLLLGDFSRTNPKASMWFHMGANMHQR
                     ERVYIGTMTSLGLNLREKTGKFLANLSTAEGANRTIGIIDEGLQKIAKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDADMAETMVDLTKDQILVQSGTAMLAQANLRPRT
                     ILQLLGT"
     gene            6426..7433
                     /locus_tag="PLJ75_04325"
     CDS             6426..7433
                     /locus_tag="PLJ75_04325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04473.1"
                     /translation="MGLNPFKESNLYDLQKQIQDIVFEKWEQISSKIKAKWEHIKKRG
                     NERLTIMIIPHSEKKIVNLHITLFTLTGIITSIVLILALTSVFIINHTSTIKEVSRLK
                     MYGSNSKIQIAKYKEEVNKLYDIFQHLKPELSYLYSLTPGNRADTLWAKGGVSGIVDT
                     EEPGSPPIEILNIEEMVQDLETTKDVLKDIKKFMRERRKVIENTPSMWPVDGFVITRY
                     GIRTSPFTFQKEFHEGVDIAAFPGAPIKVTAPGIVESIVWNNQLGLTVSIKHKYGFVT
                     AYSHCQRVTVEEGQKVAKGEVIGYVGKTGNATRHICFYQIKIGTEFVDPLPYLNKVVN
                     Q"
     gene            complement(7457..8479)
                     /gene="fliM"
                     /locus_tag="PLJ75_04330"
     CDS             complement(7457..8479)
                     /gene="fliM"
                     /locus_tag="PLJ75_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HPD04474.1"
                     /translation="MTEILSQDEIDALLTAISTGEADTTDYTAVKEQRKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVSVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAVLEIDPSITFTIIDKLFGGTGESTKISRELTDIELSVMEGIIVRI
                     LGNLREAWSNVIDLRPRLGNIETNPQFAQIVPPNDMVVLVTLETKVGEVEGMMNLCIP
                     YITIEPVISKLSAQYWYSSIRKGATDENAAIIQSRLESVELPIIVEVGEVEITMEELL
                     AIQLGDVIKLPNTKVNSDMTAKIGGRKKYKCRPGVIGNRVAVQIGEKIEDIPDELLVS
                     KRSEEE"
     gene            complement(8483..9394)
                     /locus_tag="PLJ75_04335"
     CDS             complement(8483..9394)
                     /locus_tag="PLJ75_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04475.1"
                     /translation="MKKLTITIAMLLFAVSIYSESADYLKVTLVSPKISYDENDDIIV
                     YCVVHNTAQVPVEFTIYDIPYTTFQPVVYLQNGKEAPITVDYRLKNIPAYRLVAEEEG
                     RKIILQPDEKFVYRAVCNNFYTLAKDNKYRIKVYFYPDAKDEYTIKSDNELTIAVVPV
                     PEFAMRTGIVKKERNITPSEVVLLTLNAEMNKKWDNVIKYINVEKYIYAYSEFAKRYS
                     TTNDYERLIVEQEFINFITKDRPDYLIDFSVLHEQIIAQTNVAYVDVKVKRWGPRFPA
                     TMKYRYTLEPYNNLWVIVNVDVSILKE"
     gene            complement(9474..11351)
                     /locus_tag="PLJ75_04340"
     CDS             complement(9474..11351)
                     /locus_tag="PLJ75_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPD04476.1"
                     /translation="MDPTELTLKNIYSIEGRGHFPRPDCIRLPSFNLHGQWIMAFDKQ
                     NRGIRLQWYNKENAGKIISGHYILKGFESYPVEVPFPLEADVNSAIHVKSHMAPDEII
                     KQKRFWYFCSFGVDAIKSGRLHFGACDYKATVWLNGRCLGFHEGGYTPFSFFVPEFED
                     INYLVVLVEDSISPSQIRGKQTFLHKPFLVWYTNSSGIWQDVWIEQVASTHITSLTCT
                     RDAAVCFAMSVESQENVRNDITVELKIFASQVFGKRSVLKTPVKVYRDIVVFDVFKKG
                     QVVIEVPLSLFDVWSPSHPAVHPIQVVLKKAGKEIDCIHSYFGRRDVTIQNGRILLNK
                     KNIYQRLLLNQGYYPEGLYRPVDTMQYKSDIEIMKSLGFNGCRIHQKIEDPRFLYWAD
                     MVGFLIWEEMPSYYLPLQKNMQRLYAQLQEVMARDCMHPSIITLVLYNESWGLYSMFF
                     SRKVRNSVVELFDTVKSQYHGYLIIDNSGFHHLKSDITDIHHYLQRFEDIEELYKRLV
                     DGVREAPLWTHFFMMILGKENIQTPYLKGYGDTTSPIIISEMGGFGFGIYPSEAKTLE
                     ESFQKHCSILSKFPQIQGFCYTQFTDTFQETNGVVTFDRRPKAKLLPIMQKALAKHIF
                     W"
     gene            11840..13585
                     /locus_tag="PLJ75_04345"
     CDS             11840..13585
                     /locus_tag="PLJ75_04345"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04477.1"
                     /translation="MNYDIEQFSAINKRINSSLKLSDLLTIILDTTKDMLKSEGCSIL
                     LTDPITEDLYFYIVRGGGDSLIKGEIVPKGKGIAGYVALTGTPLIVNNPYNDERFYED
                     IDSKSNFHTRNIICSPMILIDRLVGVIEVVNSTERESFTDDDLKVLMYIADSAAIAIT
                     HRQLYHDLATRLKELEIMYEISQAIAHSSPSENIFPKIMQPLASSVGVKRASILLYNT
                     NTDEFSIAASHNLPEAINLYVNKDNAPIAYRVYTSGDPLLVSNIMSDPSLASFARTHE
                     YITPSFISIPLRYKNTIIGVLNLADKVDKSSFDAHDLRVATTIANQIVEAYQNSIYLQ
                     QTEEQRRLAQEIDIAAEIQKRILPKIPHSINSHRLAAFNKSAKEIGGDFYDIFQFDND
                     KFGLLIADISGKGIPAALFMGTARNVLRAESRINNQPGQLLTHANKYIYEDSEYGMFV
                     TAFYMLIDSHNKLLTYGSGGHNNQLLVRSRSKETIHLNAKGIALGIINKAQYEEKALF
                     FEDNDLVVMFTDGVLDLLGNGNMDEGEKKLIDYSLQSLEKGPDFLISRLKNNLIVQDV
                     DKEFLDDFTVLAIQF"
     gene            13601..14581
                     /locus_tag="PLJ75_04350"
     CDS             13601..14581
                     /locus_tag="PLJ75_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPD04478.1"
                     /translation="MKKARYIFIIIGILILLFLFKTFGIQKTILQIISVGWGFWIIVS
                     IFIISHIFLTLGWKVLINHPIEGNTFLKLVLARIAGDSTSSINAFGSIAGEPLKAMYM
                     KDDIPLQIGLASVVLDRTAHSIANALMFLTGIVLSLFILNLPFYITIIALSFFIVTIA
                     IIIIIIKKQREGLLDYIVSKIPSRLKTKLITNERNKKIAELDNEIKYIFSSTSHVSRF
                     IASILLHYFAVTISCTLEIYLIVYFLKIPFNIYHALFLYVFGLFLTSVMFFMPANLGT
                     SEGSYSLALKLLGYDPSLGLTIGIIRRLRTFVWSLIGICILVIAGFKKND"
     gene            14707..15627
                     /locus_tag="PLJ75_04355"
     CDS             14707..15627
                     /locus_tag="PLJ75_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HPD04479.1"
                     /translation="MSVVSPFARFFIKRKQAKAFPHKKVLVLEGGGMRGVFLTGVLQA
                     FTDRNYFPFKLIIGSSAGALTGVAYAAHQIYLARDAFFGVLLTDSFIKITNILRPAKH
                     ILNLDWMVSAVIEGDDALNCNYLQHSIPVIITATEYNDNNPPGTLYLHSKKDDLIPSL
                     KASAAIPVLYRGFVEYKTHKLCDGGMLDPIPYKKALAMGYKEEDIVVVLTRSKGYRKK
                     EESFWIRYIVENYFKEAKYRYLVQALENRYLMYNTLLDELEHKHKKISIIYPPDDFTV
                     DRLTRDHKKILQGFEQGVQAGKNFLLSSSL"
     gene            complement(15633..16670)
                     /locus_tag="PLJ75_04360"
     CDS             complement(15633..16670)
                     /locus_tag="PLJ75_04360"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPD04480.1"
                     /translation="MRKMINVFTIFMMPLMCVLLLIHNAYGLQVSFVVGEAFVERNGK
                     QLTATVGMNVDTGDTIITKKASIVELLFLDGSAVKIFENAKVKIARVQEKEENISVVN
                     GVVKAKIAKLQKNESKKIYTPTTVCAIRGTEFFVAVSDSSNSRINMEEGSLELINPYG
                     ALTIQGDEQANAEIAKKAEADENLKNPEAWKNTEDKNFTIEPQKYADSYEEYLQTLEE
                     RSSTQSAEIAKLKKMPIKKNYSDKIEQDMQEVEEKAAMIADDYFLGDCASAAVETVLQ
                     NYKDKKQEIYDAFDKIKSQADRVLQQQKANYEAIMAVKEAYRKSYEEIKGKHKQSIEQ
                     IKEEVKKVQEQ"
     gene            complement(16976..18178)
                     /locus_tag="PLJ75_04365"
     CDS             complement(16976..18178)
                     /locus_tag="PLJ75_04365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04481.1"
                     /translation="MNAVEKPEVVIIDDDEMVNEVLKFILDDEYRVYNYYTAEDALRS
                     DVIMQADVIVTDVNLPGINGLEFIQKVHEINESIPIIIITAYNDIEIAISALKAGAFD
                     FILKPFKNDQISLAVKRAYERYKLIKENINLVGELKLKNNELEKLYAQLQARSVQIEN
                     ELDIASNLQQCLFPISFPEIKGFSFYLKFKPVEKISGDFFDFIIHDDQHFSFIFADVS
                     GHGVPAALYSAIVKTAISSIVTHEKSPSEIMNEMNQFLIQTQKTMSYNYAAIFLAHIN
                     LDNHRIEYINAGLPAPIVIHNNGDAIRLETTGPFVGIFDTSLYTAKSVKLLAGDKLLF
                     FSDGAFECPDKVDRILGEKQFQRLIDEHSKMEINELIEFLFKRVQEFCGYDRFVDDVT
                     MLGMKYEP"
     gene            complement(18179..25561)
                     /locus_tag="PLJ75_04370"
     CDS             complement(18179..25561)
                     /locus_tag="PLJ75_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04482.1"
                     /translation="MKRLLFIVLLLLLSFAGCVSTAQRDVPFDYSQVLTKEYRFGTGR
                     VIPLTFERSVNVSGTITTDGKYFIYASNRERNNFDIYLRPLNDITTFRLTVHPAKDFA
                     PDVSPDGKWCVFVSTRDDPQGDILLMKLSIDTILSQSKLEGEQPAQNLTTIKDTATGA
                     IVPIRDASPCFSPDGKWIAFSSEREGEESIFIMSRDGNKVRKITSGMQPRFSNDGNAI
                     LFTKQDGKETNIYRITIATGAIQKLTNNSSLQLSPCYGKTDDDIYFTRVEHDTNSDGV
                     VDASDNGMLYYYNVITGEEYPLTLIDEYSTAPRWVPYADGVIVYSVVEGEHINMAMIP
                     HTGIIPKQKNALRQYELALKYKEDFDDIERYQRCLFATYYFFREAVDIDSQMIIAKAL
                     YELSRDEHYTIAKQILQELSPKSKPASLYAQFILLKENNTITYLEKNIQELKLENNEK
                     LTPFFIEELADRYAAQGFTQRALTTYRDIVDTYATYSRLAYVRFKIGRLLYGGFTIPE
                     EWIVVLGSSYTYLKNEITMEVINATKQLPAAQRYAGAKEAVERYTLPIAKAIFQYIAA
                     TSLDELGQNEEAWLYLKQGLANTKKLDVAYFLINVKSGELAQKQKSDDWAGYYEAATN
                     NFQVQWKQDIRPIIEQLTEYFETNGQKLYEEGKYKESGELYKRYITMNTLLYLRRRYQ
                     DVYNINAAGAHIGYINASIPLQNTMLDTLKDEYSGQGHRLSVARMDFDKAHIYALGYI
                     YVQEALTLGSSPHAMEGRTSGELVKQLAMLKQALYHIDWALFIDDRFVDAYILKGWVY
                     QYVDELRMCCPSKKRLIDSYFSEYLWEQCIPLYEKALEANDEIITPRKEADIHTNLAN
                     IYFLLKNYTLALPHYNKAVSYQKAFTSLKEEAQFYYHYGYCLWQQGNYSGAYAAMQRA
                     YTIYKAIAQKNELSVKDQLYTLYKYFALFQQMQGNYTKAIDWYTTCVIHAARYNHEES
                     NSRLYIAIAECYRKLGNDKEALTYLELAEKNVSLTKEKQYKIGLKIVTLGPVYVFTVN
                     QDDIIIGEGKIVSELSSFEMQLLIHSMTAEIYISNGRFTDALTRYDKIIKLAGNRKGS
                     LFLDVTFSALQNAGYCAAQSGQIADAVRYYEQALAIAQKESIAVDKVYRMMLQWAYCY
                     TAGSYGAKDALHQTDVALDTIEHYTTSMYERVYAQLSNNYINTMKAQKKRPVPEDIEK
                     LSQQATKQVEGSTISFNALYALLLMQKATIMANNQSSLGQSTATASLYSQASALLSQA
                     IDYNANDPYHQEFVIKLLINRGICNIATGKVKEGYDDIISARFKANAVQSKELVWFAE
                     ATVWDYANRYNSLMQHIENPDSLIAGVLSRVELLPQLYARYAGFIYNLYDRYTDYLLN
                     AGKYREAFTAQQRKQIVALAMSGATLNDFHNKGDDEFFTHYKKTCFTVYKLERALTQK
                     IVADGKKELIDTIKENLSMQYKKIDTFRKNATDFMAPYVGAYRATRYDVPVLHFYDYN
                     GKLYCWIAYKNTVDWRLISDEINAKTITADIEAVLQNYGNRGYVILNDSFMTIHPLLD
                     TTLLYKLGCIPCAARAPEVEKKVWLPVTSIATDMRLSGIPNVINDIHEAEYIVDSENK
                     LYAVTARYCFTTVIECSAVVKNIQGLSLNDLLLLSDAALYADVATIIGYYAGGRVETI
                     VRAISSDDANLLHEVNTPLLLLGKTPSDIKQRESNALVVAKSSSQLFNNALGKGDYTK
                     ALLALERWKQTGLHDNEYTIHKIKVLLYQGKIDDAVALIQLIADNDSIARSYLVYAYL
                     LQGHMEEALKALKGNAALQGTLDNEVYAALIEAWNGNQPLAEYDTFDNHEKSLLPLNQ
                     LRLLYIQFAALLKEDDKAGKALQQFTADYILSQRELCLVYSLGYAIDSYPIATNAGML
                     INSLKNNKYSVDTLTSMDYDALSLFVIHEIVLHADINEEAWLKMLMAAQNALQYAQWI
                     DIQFYTIKIADNCNKSVPQRSIQLIESIPSQKGKPAWTFVLQHIVAYQKTGKFKQAHT
                     LCTAFHDLPFQIKRQWYMRYLYSVIAMGKTKEAGKLLDESGMISWDKSLYDLFLMNIQ
                     LQKMAIAKKTDESVFKAAENAIARFIVSLSSDMAGFYYSDYPYLLDKLFNYMVSYSMS
                     RGNQKDALQYAELHRMIYGIAVLSNNEYFKQQYDNLVRNAIPITAIQKKLPKDVALLY
                     TAKNENDIFAWIITRTYIQPVRLSNCYSEVKDLVYEYYNNLGLLQPVGGIENNIDEKM
                     KPVIKECAPYSRVIIFADVYTDAIPFEAILYHQSGSVVAFMPSLVAAMGSTRVIPSVI
                     AINIGDSNRDSIIAAVKQSGVHYGFAISSSIIITDKPLWYSDNKKNFVMDDALVKPGC
                     LIIDLTIMQGQSYNTGLVLMQKGIGGFITFNCAVIDVNRLLFLREFLTALKSKPSFTA
                     YADAFVYVIRDKRYKHPANWVGIRFYSSSMNYLYKGAD"
     gene            complement(25558..26157)
                     /locus_tag="PLJ75_04375"
     CDS             complement(25558..26157)
                     /locus_tag="PLJ75_04375"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HPD04483.1"
                     /translation="MKKNMVAIALACIVCIVIVMSGCTSSGCIQDMPSCLEIYPPAIN
                     ITGEKTALERQLIGEYHELEQDAWVTSTVMTPSKGNRKAVVNADDELLNAIQIRELNS
                     DTIRLYKDKELIGEQNNGYIAIVDEKKLQSLQEADVIRTVVQQENNARSVIFVKSSGA
                     ARSSEAKAIQFAVEFAKEQHAKAKKGDWIQNNDGRWVKK"
     gene            complement(26154..28850)
                     /locus_tag="PLJ75_04380"
     CDS             complement(26154..28850)
                     /locus_tag="PLJ75_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04484.1"
                     /translation="MKKVAYYLSIGVAGFIVLLFIGIIGVNIYITDSKATRFLISQIE
                     SSLNATASLQVTHFSLFHGFEIKNCKLFVLGDTTPLLQFDTFKLKYSLLPMLIGKVNI
                     YEVGLYNPSIVIDEHKGVWNFQRMLKPSEKKEKEEKEEKQKVEAGEAISLPIPVEFLF
                     NFIVQNLSITVHGSTYELRCHDFSSYITVHAPSMRSIPLNVKALALFDTMDIAINPQK
                     TLQLSFISKDVKAGPPLLFSFHVAYNPDQKQLSSNFICGTYTTPLRFVQKHVAPLNAK
                     IEYDIIYNPSTDVLRCNTFMITFNQSTWVSFAGSILHVNKSPEISFAMLDSNIRLHEL
                     YTYTSKLIGPSPYFHGTVSLFPLMITGKGNNFTVTGAISGENVAVATGDFSITVPSFT
                     IPYTVAMSNAYVTGSLRIHIPHFYYALGEKSKDNSADLVCDINYNRNTGFVQIHRFVM
                     VHRDAASAKEAIRCEMDGTVSLHDTIQARIAIHPLMINMPVLTHTLPAHLKQALSSSP
                     ITKPISVTLNTTLQSSKKVNAIELATIFAIPDYDIDDLKLTASIAHKPEQQKITISVL
                     ELSSSKKNMAIQAHGNVVMGNDIMIDVSTGLTVAPAKPVAFADYSLSGQLAVAMHLKG
                     TVQKLSAKGNIVSKKLQIESKPNKLYVGPVDIDIPIMYTMGESFEIPFDVADSMNIAL
                     FKPEVNVSIGRIVAKHPSRNIAFEYMSSCKGYIGCKRNTLEIKNVQATVMGGTVTLKE
                     LLFNLADLKPENMGFRVAFNVNDIDIGKLDDPSSLRIHHDSLLSMNAIMQGKNLSTGQ
                     NLDISGTATIYKVGEQFANRLFKGLNEEKGKSKLGMAQIAVDNSLIIAGFDFYIDKGL
                     VYPTVVFKRKILGALVTVNNERVRYERIPVMEFFNKVREESL"
     gene            29082..29456
                     /locus_tag="PLJ75_04385"
     CDS             29082..29456
                     /locus_tag="PLJ75_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04485.1"
                     /translation="MSTLKDLFSLEYDKDTKILLGTIKKPILTKEEANEILEFVKQDM
                     QNYPDHQHYILNVLTVKEVSNPIIGVLMKSLQFWKKTRGYALLVITDDLLQKIMIEQP
                     AMFDFFAVFPTVDEAIAFTKKS"
     gene            complement(29459..>31021)
                     /locus_tag="PLJ75_04390"
     CDS             complement(29459..>31021)
                     /locus_tag="PLJ75_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="activase"
                     /protein_id="HPD04486.1"
                     /translation="PDSFMLHYVKWIMGTKPFLILELDSHTADAGVDTRVEAFLDIVD
                     GYRQKCIDKQAERYDNGLRFIIENNGLFVKNSYTGSKIPIRNNPKVKVLLSNMGRLGV
                     ELLAASLRSTGINAVAMPVPDNRTLQLARQFVSGKECLPSHLVLGSALNFFSSTHYRK
                     DEIYLLFVPTTQGPCRTGQYFVFYQNLFKDLRLENVCVITLDSSNSYNELGSHFSKHA
                     WWGVVISDYMKDIETSLRACAEDPVSAMALYDQLWQELISIAEKDVTKVIPALQRIAA
                     AIQQIPLKKTIEQCPRVLVVGEIYVRRDDFAVDELIKHFSTKGIIAKVSGVSEWIYYC
                     DFIREYEYKKAIKAAPWYKKLFIPETKKLLLFYIEQWYKHGVENKIKEALMPTSLIPE
                     TPHNMHHIMKMSQQHFVNLELQSEITISSGVAASAMEDGYSGIVNISPFACLIGRVIE
                     GILTPWARTKGYPMMSVEIDGNALPPTTLNKLDIFMLNVMRFKKQTAEAAIVDYPGME
                     NVAVDRKLVKEL"
     assembly_gap    31022..31121
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31122..33313)
                     /locus_tag="PLJ75_04395"
     CDS             complement(<31122..33313)
                     /locus_tag="PLJ75_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPD04487.1"
                     /translation="MKTSFSGNKCAAGTGEFFKQQLARMDMTLNDVASIPADSKVMKL
                     STRCSVFMKSDCTHRLNKGEATKGDIVLSLSNVMAIKVVDFLKKARIQKGVVVLTGGI
                     TQNPFILQFIKEKLPNVTFLVPESAPYFEAYGAALLAQTYGGLIGDNLFEKHTVSFPR
                     FSNLTSALDKVTYIPSKRSRAKPGGEYILGVDGGSTTTKACLIDIHTHEIVADYYGRT
                     HGDPVKALKQCIEQIQQQLYEQIGDASITISLAATTGSSREILGVFLETPAIYNEIIA
                     HSVGTTYFKPDIDTIFEIGGQDAKYVYLKNGVPIDYAMNEACSAGTGSFLEESAQGDL
                     NIAHAWEIGDIALQAEEPLKFGEHCSAFINSDIRKAIQQGASKEDITAGLVTSIVANY
                     LNRVVGNRTIGNAIVLQGGVAKNKAVPLAFAMLLQKNITVPPDPELMGCFGVAMVALQ
                     KLQSGLLQKKLYSLDAIVQTEIIYEREFTCKSCDNYCPIRVLKVNGNKYMFGGRCNKY
                     ANMRKKRIINDDEVVDFIEKRNQLLFEECAPQPLTGSSPIRVGIPLCFSTYSLWPLYA
                     WFFSQLGVAIVTSDEISDEGKAKAESAYCFPAEIAHGAVKNILDKGVDYIFLPHMKEM
                     KSFVDDARATFCPITQGLPYYIEKAFDELPEEKILSPVISLKLSDAMTEQSFVDVAVT
                     LGFSAEQGMSAYHVALDKQKEYFVKAQKLGKEALEFSKRLSKPAIALLG"
BASE COUNT         9924 a         6940 c         5674 g        10714 t
ORIGIN      
        1 gggctccact tttctgtaag tagatctgca gggccaccat caacatattt aagctctaac
       61 accggcacag ggtctttttt aggatcagga ttctttttcg gaattgtttc caattgccaa
      121 tcagcctgtg tttcaaaatc ttctactacc agagcactat attgctctgc gctaatatcg
      181 ggttgcacat tggttactat cctggattgt actttttcag ggaaaaatcc cacaagcacc
      241 atcccaacaa taactactat aattgctgac aatcgtatat atctcatatt tatgcctcca
      301 ggtctcattt gctcatcatt ttcattatca ccagttatcc ctgatgcttg tatcaccagt
      361 gaactctgat agatcagtaa ttactctgaa attatccagg tagaaataaa acgtccctgg
      421 tacttcataa atgtcacttt ctacatagaa ggaaacaaaa tggagatttt tatcgagcaa
      481 tgcataccct ggactctgtg gcaaccaacc cggtacaatg actgacatct ctctccatcc
      541 ccagaaatcc aatcttccaa tttttatttt gtgaatggct cctttataat cgcgtagctt
      601 cacaaaaagt gtatgcctga attttcgtcc taatgcccaa accttgatct cttttgcttt
      661 accacgaact atatactcat ttgggggaaa aacctcaact ctatcaaaac ctctatccat
      721 aaaataagtc ttaacaccta atacatattt attctcatgt gtgattccat tttcatcagt
      781 tatcgtatca ggataattaa cctcattgcc attttcatca attggtcttg gtttaccggg
      841 aatcttcctg atttttgttt ctccaattgg acatgttgcc attgcccgcc aatcttcact
      901 tgcttcaaag tcatccatcc aatcttcaac taaaccagtt tgttgctttt tctgttgttg
      961 tgcctgctgc gcttcctgcg cctgcaccac tatattgcct ccaccgactg ctaccattaa
     1021 accaccgatg gcaagcgaca atataaaaga tctgacctcc cttctcattg tcgactcctt
     1081 tttaaaaaga ttatgaaacg tttaatacct attaatagta tttgcttttc accataatac
     1141 tatgatatca tatacttaat cggctaaaaa agtaaaaata tttaataatt attcttatta
     1201 tgttcgatag tatctgatat tatatcgtta ctgtcaagaa attaatattt atttattgct
     1261 atcaaaaaaa atttatacac aattgttatt gattttttta ttgccacaaa tcactatcac
     1321 cataatcaac ttatcaccat ttttctatat taaaaattaa aagtgagtgt tatggatatt
     1381 gctgtctgtc tacaacataa aaaaccattg ctgtcatttg aagtgtttcc acctaagact
     1441 tcatcagggg aggaaaatct cttcaaagaa ctatcgatct tggtgcacta taaaccagac
     1501 tttatttcag taacctatgg agctggcggt tcaacacgag ataaaaccct tgagattgcg
     1561 attcgcataa aaaaatcatt ttcaattgaa ccacttgttc attttacctg tgttggccag
     1621 agtaaagatg acattgtttc ttatcttcat tatgtgaaaa cactgggatt tagcaatatt
     1681 ttagccttgc ggggcgatcc cccaatgggc gaaacccatt ttaaacctca tcccggcggt
     1741 tttacccatg caaacgaact ggtatcattt atcagaagta tgaatcactt taccattgga
     1801 gtagcaggat atcctgaagg acatattgaa gcacccgatt tagataccga tatagccaac
     1861 ttaaagaaga aagtagatgc aggagctgat tttatcatta cacagctttt tttcaataat
     1921 gataaatttt atacattttt ggataagacc ataaaagcag gcattactat tcccatccta
     1981 cctggcataa tgccattaac cagtattgca caacttacta aaatcacaac aatgtgtaat
     2041 cctgatattc ccaattcatt attacaggag cttgagaaat gttctcagcc cgatgatatg
     2101 tgtaaggttg gtattgaatt taccatacag cagtgccagc aattacgttc gtatggagtt
     2161 ccgggttttc atttttatcc cttaaataaa tctgctgcaa taaaattaat tctggacgag
     2221 ttctggcata cataacacat tcagagattt ttttatcacg tggtaaacta tttttactat
     2281 tttaaggggg caaaaccatg aaatcctatc cattttattt ttatacaggg ataatgctca
     2341 tagcttttat gcaactatgc attctatttc atgtttcgca tgttacaata tgggctacgc
     2401 caaccctgtg gacaggtctt atcttactta ccgatggaat acttgccaaa agcaatcgct
     2461 cattaataaa aaaccgaact atcgtaaagc tatcactgtt atcatttctt tgctggtgga
     2521 tgttcgaatg gttcaatatc tttttatcta attggcatta tcagggactc attcaatctc
     2581 tgcctatccg atattttggc tatatctggt cattcagcac tatcttcccg ggagtattgc
     2641 tggtatatgc aatcatagca accatactgc ctaaactttt ttttaaacct atacatataa
     2701 catcatcgtt ccttgttgta tcctttatta ttggactatt ctttatcttt ataccaatca
     2761 tccctttttc tatgcattat acaaaccgtg cagctgaccc cgatctattc ttctggctta
     2821 catgggctgc caacattcat tatagtgagt ttacagccgg ttttacctgg ttaggattta
     2881 tctttttact ggagccagtt aattatttac taaatcgtcc ttccatctat gctgacctta
     2941 tagtaggcaa atacacgcga ctcgtgtctt tgttggcagc cggtttgctc tgcgggtatt
     3001 tatgggaatt ctggaattac tgggcagcaa caaaatggat atataccgtt cctattttac
     3061 cctccctaaa attgtttgag atgccaatcc ctgggtatat tggcttttta gcatttgcat
     3121 gggagatata tgccattact gcacttgttt atcctaaagc actatacacc ctggaaggtg
     3181 aacgttaatc acatggaaga tattattgtc attggacata aaaaccctga tactgattca
     3241 atctgcgctg catactgtta tgcacaatta aaaaatagtg tcgattctac caatcgttat
     3301 cgggcatacc gttgcggcaa tattaaccgt caaacccgtt ttgtatttaa ccaaattgga
     3361 ttgcagccac ctgatttaat caacaatgtg tatcctaaag taaacgatat tatgacaaaa
     3421 aacgttatta ctgcatatgc tgatgatcct ctctattcaa tcataaagca tcttgagcag
     3481 gatagaatac gtattatacc tatagtggac agtactaacg tgtttaaggg tattgtgagc
     3541 tttaatgaaa ttgctcaatt ttattttccg gtaacatcca tagcaaagcc agtttattta
     3601 ttcaaacctc aaaattttaa tcttaccatc ccgggacggt ttattcagca gggaaaagat
     3661 aaggaatttg aagcagcaat agttattgga gctatggaat caacacggtc tattgaagaa
     3721 ctacacaatg ttatccctga aaaaacggta ctcttagtag gcgatagaaa agagataatt
     3781 gaatttgcga tcaaaaataa ttttgctgca ctattgatta ccggagtgaa tgattttacc
     3841 tatgacacta ctcactataa aggatgtatt tatctttccg aaatagatac ctacaatacc
     3901 ttgcgaatgt taacgttaag cataccatgt cattctatta tgcaagatgc tgatacaata
     3961 tccccggatt tgccagtaga gcaggcacgc gaacaactgt tgcttaatga ataccgggga
     4021 ctacccgtat gtgacaatac tatcctgaaa gggattatta cccgttcgga cattatcaag
     4081 cactatgcaa aaaaggtaat ccttgtagat cacaatgaat tatcacaagc agttgatgga
     4141 gctcaaaccg ctcaaattat tgaaattatt gaccatcacc ggttgggaag cttaaaaacc
     4201 acaaccccaa tatttgtgta ttccaaacca gtgggaagta cctgtacact ggtttatgaa
     4261 ctttttaaat ataacaatgt taaacctgaa ccgcatatcg cacttctgct atgcagtggc
     4321 atactatctg ataccaccat gctattatca cccacaacta ctgaaactga caaaaaatca
     4381 ttagaagaat tggcagcgat agctggtatt aactacaacg attatgggca aaagctttta
     4441 tcctcaaccg aaagtttgaa aaccattgat cccatatatg cggtaagcaa tgattgtaaa
     4501 atctatgaag aatatggtat cgcggtagct atatcacagg ttgaggtgat aacacttgat
     4561 ggtatcaatg aagtgaaaga atcattgtta tctgcactgg tacaatttgc ccaaagcaga
     4621 aatgttcact gggcattatt attggttacc gacatcacca ctcagaatag catcctgtta
     4681 tcaactgact ttaaaggtga ggttcatttg aactatcccc ccattgagcc ccacatttat
     4741 agtttgccgg gcattttatc ccgaaaaaaa caattactcc ctgaaatatt gcgcatttta
     4801 gaggaactat cgcacaaata aaagtgcccg cttaaaacag cgggcactat gtttagtacg
     4861 ggtattccct gatagtagta tacgttttta tcttatgttc ctaaaagctg taagattgtt
     4921 cttggtcgta aatttgcctg agcaagcatc gcagtaccac tctgcacaag gatctgatcc
     4981 ttggtcaaat ctaccatcgt ttcagccatg tcagcgtcac gtatacggct ttcagatgcc
     5041 tggatatttt cataagcatt catcaaacct tttgctgcat gctccaatcg attatagtag
     5101 gcacctaaat cagcacgctg cttggctatc ttctgtagac cttcatcgat aataccgata
     5161 gtcctgttgg ccccttcagc agttgataga tttgctagaa attttccggt cttttctctt
     5221 agatttaatc ccagcgaagt catggttccg atatatacac gctcacgctg atgcatgttg
     5281 gcacccatgt ggaaccacat acttgctttt ggatttgttc ttgaaaaatc accaagcaag
     5341 agtttgaact tgttgaattc tgcctgagat gcaattcgat caatttcatc aaccagagcc
     5401 gatacttcaa cctgaataag ctgtctgtct tcaagagtat agataccatt agatgactga
     5461 accgcaagca ccctgattct ttgcaatatg gttgcagttt gatttaagta tccttctgct
     5521 gtttgaacaa atgacatacc atcttcagta tttcgctcag cctgccgcaa accctgaatc
     5581 tgagtacgca ttttttcaga tacagccaat cccgaagcat catcgcctgc tcggttgata
     5641 cgcataccgg atgataactg ctccattgaa cggttaacat cccagtgttt aaactttagc
     5701 accttatttg aattaatagc gcttaagttg tggttaataa tcatacacac actcctttgt
     5761 gttaaaattt tcctgatctc cctatcagga taaaaccggg ttgttgatct aaaaaaagag
     5821 aatacccttt ttttacttca ctacaacccc ttatccctca tttaagttgc taccttttta
     5881 aaataaaaga aaaaatgaag aatatggcgt tgcagggatt gccttattag ttttcaaaga
     5941 accttacagc ttattgtgat gttcggaagc tctgtttaaa gaattaaagg caattatatt
     6001 tgtttatcta tggtttatgg cgtttacggt tatttatact atattatgta tgcatttcat
     6061 ctatttaatg aatttttcta tatattttcc cttattcaac atttcggaat gtgaactatt
     6121 tgctttagca aattttttaa tttctattca tttcatgtaa tttttaaatc tatacgtcgt
     6181 tgtctaccat gaactttttt gaacacattg caatacgccc tttctgtcac tcacaatgaa
     6241 gctttaacaa tttgaccata aaaaaagaat aaacttgaca ataaatggtc gattattaaa
     6301 atagacaggt tatttttatt ctatacatgc aaagtcatgt atagaatatc tgctactttt
     6361 ctatacattt tgtttactat tgtttgataa ttactatact actatgcaac aggtttaaca
     6421 atactatggg attgaatccg tttaaagaat caaatttata tgatctccag aaacagatac
     6481 aggacattgt ctttgaaaaa tgggaacaga tcagttctaa aattaaagca aaatgggagc
     6541 acataaagaa aagaggaaat gagcgtttaa ccattatgat catccctcac tctgaaaaaa
     6601 agatagtaaa tttacacatt actcttttta cacttaccgg gattatcaca tcgatcgtac
     6661 ttatattagc gctcacatca gtatttatta taaaccatac atcaacaata aaagaagtat
     6721 cccgtcttaa gatgtatggt tcaaattcca aaatacagat tgcaaagtat aaagaagaag
     6781 taaataaatt atatgatata tttcagcatt taaaaccgga gctatcatac ctctattcat
     6841 taacacctgg caacagagcc gatactctct gggcaaaagg tggcgtatcg ggaatcgttg
     6901 ataccgaaga accgggttcg cctccaattg aaattctcaa tattgaagag atggtacagg
     6961 atctggaaac tactaaagat gttctcaaag acataaaaaa gttcatgcgt gaaagacgaa
     7021 aagttataga aaatacacca tcaatgtggc ctgttgatgg attcgttatt acccgctatg
     7081 gtatacgtac atctccattt acatttcaga aagaatttca tgaaggagtt gatatcgctg
     7141 catttcccgg agcacctatt aaagttaccg ctcctggtat agtggaatca atagtatgga
     7201 acaatcaatt aggattaact gtatctataa aacataaata tggatttgtt actgcctatt
     7261 cacattgcca gcgtgtgacg gttgaggaag gacaaaaagt tgcaaaaggt gaagttatcg
     7321 ggtatgttgg taaaaccgga aatgccacac gccatatctg cttttatcaa attaagattg
     7381 gtacagaatt tgttgatcca ttaccctatt taaacaaagt agtaaatcaa taagatattt
     7441 gtaagtaata agtaagctat tcctcttcac ttcttttgct gacaagcagt tcatcaggta
     7501 tgtcttcaat cttttctcct atttgcactg ctactctatt tcctatgaca cccgggcgac
     7561 acttatattt tttccttcca ccaatttttg ctgtcatatc tgaattaact ttagtgttgg
     7621 ggagtttaat aacatcacca agttgaatag ccaacaattc ttccatggtt atttctacct
     7681 caccaacttc tactattatg ggtaactcaa ccgattctaa cctgctttgt attattgcag
     7741 cattttcatc ggtagcaccc tttctgatac ttgaatacca gtactgagct gatagctttg
     7801 aaataacagg ttctatagtg atatagggaa tacataggtt catcatcccc tccacttccc
     7861 ctacctttgt ttccagcgtt acaagaacaa ccatgtcgtt aggaggaaca atctgtgcaa
     7921 actgcggatt tgtttcaata ttacccaatc gtggacgaag atcaatgaca tttgaccatg
     7981 cttcacgaag attccctaaa attctgacaa taatgccttc cataaccgat agctcaatat
     8041 cggttaactc acggctgatc tttgttgatt caccagtacc gccaaaaagc ttatcgatga
     8101 tagtaaaggt tattgaagga tctatctcca gtaccgctga acctttaaga ggatccatat
     8161 tgataactgc aagcgtagtg gggttgggaa tagaacgtat gaattcctca taggtaagct
     8221 gatcaacaga agccacatgc acacttacca aagcacgcaa ttgcgctgac agagcagttg
     8281 ttgttaaacg tgcaaacgtt tcatgcatca tctgcagggt acgtatctgg tctttggaaa
     8341 atttatcagg gcgccggaaa tcatatatct ttaccttgcg ctgttcttta accgcggtat
     8401 aatcggttgt atccgcttca cctgttgaga tggcggtcaa cagggcatca atttcatcct
     8461 gtgaaagtat ctctgtcata gattattcct taagtattga tacatctaca ttaactatca
     8521 cccataaatt gttatatggc tccagcgtgt accggtactt catcgttgca ggaaagcgcg
     8581 gaccccatcg ctttacctta acatcaacat aggcaacatt cgtctgcgct attatctgtt
     8641 catgaagcac tgaaaagtca atcaaataat cagggcgatc ctttgttata aaattaataa
     8701 attcctgctc aacaatcaag cgttcataat cgtttgtagt agaatagcgt tttgcaaatt
     8761 ctgaataggc atagatatat ttttcaacat ttatatattt aataacatta tcccactttt
     8821 tgttcatttc tgcattgagg gttaacagta ccacctctga aggggtaatg ttacgctcct
     8881 ttttaacaat accggtacgc atggcaaatt ccggaaccgg aacaacagct atagtaagtt
     8941 cattatctga tttaatggta tactcatctt tagcatccgg ataaaagtat accttaatac
     9001 ggtatttgtt atcctttgcc aatgtataaa aattattgca cactgccctg tacacaaatt
     9061 tttcatcagg ttgcaaaata atttttcttc cctcttcttc tgctactaac ctgtaagcag
     9121 gaatattctt taaccggtaa tcaaccgtaa ttggtgcctc ttttccattc tgcaagtaca
     9181 caactggctg aaacgttgta tagggaatat catagatggt aaattcgacc ggtacctgtg
     9241 cagtattatg aacaacgcaa tacacaatga tgtcatcatt ttcatcgtat gatatctttg
     9301 gacttaccag cgttaccttc agataatcag cactttctga ataaatactc actgcaaaca
     9361 gcagcatggc gatagttatt gtcaattttt tcatagtata tagtataata aaagtaagct
     9421 cttatgaaaa ggttgtacct taccgttttt atcgactact ataccgatat ttgttaccaa
     9481 aatatatgtt ttgccagcgc tttttgcatt attggaagca acttagcttt tggcctgcga
     9541 tcaaaggtaa caaccccgtt tgtttcctga aatgtatcgg taaattgtgt atagcaaaaa
     9601 ccctgtattt gtggaaattt tgataagata ctgcaatgct tctgaaagct ttcttcaagg
     9661 gtttttgcct ccgaggggta tataccaaaa ccaaacccac ccatctcgct tatgataatc
     9721 gggcttgttg tgtcgccata tcccttgaga taaggagttt gtatattctc cttacccaga
     9781 atcatcataa aaaaatgcgt ccatagcggc gcctctctga cgccatcaac cagccgttta
     9841 taaagctctt caatatcttc aaatcgctgc aaatagtggt gtatgtcggt aatatcgctc
     9901 ttaagatgat gaaatccact attgtcaata ataaggtacc catggtattg gctctttacg
     9961 gtatcgaaaa gctctactac actgttgcgc accttccgtg aaaaaaacat tgaatagagt
    10021 ccccagcttt cattatacag tacaagcgta atgatggagg ggtgcataca gtcgcgcgcc
    10081 ataacctcct gcagttgcgc atataatcgt tgcatattct tttgtaatgg gagatagtaa
    10141 ctgggcatct cttcccatat taaaaatcct accatatccg cccaatataa aaaccgcgga
    10201 tcctctatct tttgatgtat gcggcagcca ttgaatccaa gcgatttcat aatttcaata
    10261 tcagatttat actgcatggt atccacagga cgatataatc cttccggata gtatccctga
    10321 ttgagcaata accgctgata gatatttttt ttattgagca gtatcctgcc attctgtatg
    10381 gtaacatccc tcctgccaaa atagctatgg atacagtcta tttctttccc ggcttttttt
    10441 agtactacct gaatgggatg tacggcagga tgtgaagggc tccatacatc aaaaagcgac
    10501 agcggcactt caataacaac ctgtcctttt ttaaatacat caaaaacaac tatatcacga
    10561 tacactttga caggggtttt taacacactc cgttttccaa acacctgtga tgcaaagatt
    10621 ttaagctcaa cggtaatatc gttacggaca ttctcctggc tttcaacgct catagcaaag
    10681 caaacagcag catctctggt acatgtcagt gatgttatat gtgtgctggc aacctgttca
    10741 atccacacat cctgccatat tccgctacta tttgtatacc acaccagaaa tggcttgtga
    10801 agaaaagtct gtttgcctcg tatctgtgat ggcgatatgc tatcctcaac aagcacaacc
    10861 agatagttga tatcctcaaa ttctggcaca aaaaacgaaa aaggcgtgta tcctccttca
    10921 tgaaaaccca gacatctgcc attaagccat accgttgctt tataatcgca tgctccaaaa
    10981 tgcaatcgtc cactttttat agcatcaaca ccaaaggaac aaaaatacca gaaacgcttt
    11041 tgtttaatta tttcatcagg tgccatgtga ctttttacat gaattgcgct gttcacatct
    11101 gcttcaagag gaaatggtac ctccacaggg tatgactcaa atcccttaag tatatagtgc
    11161 ccgcttataa tctttcctgc attttccttg ttataccatt gtaaacgtat accacgattc
    11221 tgtttatcaa atgccataat ccattgccca tgtaaattga atgaagggag ccgtatgcaa
    11281 tcaggtcgtg gaaaatggcc tcgtccttca atgctgtata tgttttttaa tgtcaactct
    11341 gtcggatcca tcttgttctc ctgaaacagt attgtttctc aatgtcattc cgtgcctgtc
    11401 acggaatcca gatgtgtaac agtatgtcct gaaatgatcc tgaaaccagt tcaggatgac
    11461 gctcctcaca ttgtcatgct gagggacaca gtcccgaagc atctattcgt tcacaaaacg
    11521 gcgatattct tcggattcgc ttcagaatga ctatcctctc acaatcagca taccattagc
    11581 taacgacaaa tataatcatg taatactatc gcacctatac atttttttca tttacgcaac
    11641 gctgatgcat gctgcaaata cttttttatg gaagttatac tctaacataa aaaacatgct
    11701 tttactgtac cattatacta aaaagccgtt gtggtaatca aatctatttt taatagtaac
    11761 tatcgaaaaa agatagacat tttttaaaat tatattctaa tagtactcta tattaaataa
    11821 ttaatgaata taattactta tgaattacga tatcgagcaa tttagcgcaa ttaataagag
    11881 gattaactca agccttaagc tatctgatct tttaaccata atccttgata caacaaaaga
    11941 tatgcttaag tctgaaggat gttctatact gcttaccgac cctataaccg aagacttata
    12001 tttttatatt gtcaggggtg gcggcgattc actcataaaa ggggagatag ttcctaaagg
    12061 caaaggcatt gccgggtatg ttgcgcttac cggcactcca cttattgtta acaatcccta
    12121 caatgatgag cgcttctacg aagatattga tagtaaaagc aactttcaca cgagaaatat
    12181 catatgctca ccaatgatac tcattgacag gctggttggg gttatagagg ttgtcaacag
    12241 taccgaaaga gaaagtttca ccgatgacga tcttaaagtg ttaatgtata ttgcagattc
    12301 tgcagccatt gcaataactc accgacagct ttaccatgac ctggcaacac ggctaaaaga
    12361 acttgaaatt atgtacgaaa tatcacaggc tatagcacat tccagcccat cagaaaatat
    12421 ttttccaaag attatgcaac cgttagcatc gtctgttggt gttaagcgcg catcaatact
    12481 gctgtataat acaaataccg atgagttttc tattgcagcc tcacacaatc ttccggaagc
    12541 tataaacctg tatgtaaaca aggataatgc tcccattgca taccgcgttt acaccagtgg
    12601 cgaccctcta cttgtcagca atatcatgtc cgatccttca ttagcaagtt ttgccagaac
    12661 tcatgaatat ataactcctt cgtttatatc aataccactg cgatataaaa ataccattat
    12721 tggtgtatta aaccttgcgg ataaagtgga caaatcaagc tttgatgctc atgatctgag
    12781 agttgcaaca accattgcta atcaaatcgt agaagcatac cagaattcca tctatttaca
    12841 gcaaactgag gaacagcgaa ggcttgctca ggaaattgat attgccgctg aaattcagaa
    12901 acgaatattg ccaaaaattc ctcactctat caattcacac aggcttgcag cttttaataa
    12961 atcagcaaaa gagattggtg gagattttta cgacattttt cagtttgaca atgacaagtt
    13021 tggtttatta atagccgaca tctccgggaa aggtattcct gccgcgcttt ttatgggtac
    13081 agcgcgcaat gtattgcgtg ccgagtcgcg tatcaacaat cagcctggac agcttttaac
    13141 ccatgccaat aaatatatct atgaagattc tgaatatggc atgtttgtaa cagcttttta
    13201 tatgcttatt gattcgcata ataagcttct tacgtatggc agcggaggac acaataatca
    13261 actgttggtc cgctcgcgca gtaaagaaac aatacatctt aatgctaaag gcatagccct
    13321 gggtataatc aacaaggcac aatatgagga gaaagcgcta ttttttgagg acaacgattt
    13381 agtggttatg tttactgatg gggttcttga tcttcttggg aatgggaata tggatgaagg
    13441 ggaaaaaaag cttattgact attcattgca atcacttgaa aaaggacctg atttccttat
    13501 atcccgcctt aaaaacaatc ttattgtgca ggatgttgac aaagaatttt tagatgattt
    13561 taccgttctt gctattcaat tttaataatc tggaaatttt atgaaaaaag cccgttacat
    13621 atttattatt attggaatac ttatactgtt atttcttttt aaaacattcg gcatacaaaa
    13681 aacaatattg caaattattt cagttggatg gggtttctgg ataattgtta gcatttttat
    13741 aataagccac atctttttaa cattagggtg gaaggttctt ataaatcatc ctatagaagg
    13801 aaatacattt cttaaacttg tactggcccg cattgctggc gattcaacat catcaataaa
    13861 tgcctttggc tctattgcgg gtgaaccatt gaaagctatg tacatgaaag acgacattcc
    13921 attgcaaatt ggtctggcat cagtggttct ggatcgaact gctcattcaa tagccaatgc
    13981 tttaatgttt ttaacgggta tcgtactgag cctgttcata ctcaatttgc ctttttacat
    14041 taccatcatt gccctttctt ttttcatagt aactatcgcc ataattatta ttattattaa
    14101 aaagcagcgt gaaggcttgc ttgattacat tgtttccaaa attccttcac ggttaaaaac
    14161 taaacttata accaatgaaa gaaacaaaaa aattgcagag cttgataatg agataaaata
    14221 tatattcagc agcacatcac atgtatcacg atttattgct tcaatcctgc tccattattt
    14281 tgctgtaacc atttcatgta cccttgagat atatcttatt gtctattttc tcaaaatacc
    14341 atttaatata taccatgcat tatttttata tgtctttggt ttgttcctta cctcggtgat
    14401 gttttttatg cctgccaatt taggaacaag tgaaggctca tattcactgg cattgaagtt
    14461 gctgggatat gatccttcgc tgggacttac catagggatt atacggagat tgcgaacatt
    14521 tgtctggtca cttatcggta tatgtatact ggtaattgcc ggctttaaaa aaaatgattg
    14581 agcacccctg tttcagaaat tactttattt ttttatccat gcaatttttt atggttgaca
    14641 ctgctctcca ttgcattgtt ttaataataa caaaataaaa aacacttgtt caggaaggag
    14701 catctcatgt cagtggtatc accttttgca cgttttttta taaaaaggaa gcaggcaaag
    14761 gctttcccgc acaaaaaggt tcttgtactt gaaggaggcg ggatgcgagg cgttttttta
    14821 accggtgttt tacaggcttt taccgataga aattatttcc ctttcaaact cattattggc
    14881 tcatctgcag gagccttaac tggcgttgcc tatgcggcac atcaaattta tctggcacgc
    14941 gatgcctttt ttggtgtttt gttaaccgat agttttatca aaattaccaa tattctgcgg
    15001 ccagcaaagc atattttaaa tttagactgg atggtttcgg cagtgataga aggtgatgat
    15061 gctttaaatt gcaactattt acagcattct attccagtca ttatcacagc aacagaatat
    15121 aatgataaca acccgccagg cacactctat cttcattcaa aaaaggatga cctcatacca
    15181 tcattgaaag cctccgctgc catcccggta ctgtatcgag gctttgttga gtataaaaca
    15241 cataagctat gtgatggtgg catgcttgat cccattccct acaaaaaagc tttagctatg
    15301 ggatataaag aagaggatat agtggtagta ttgactcgta gcaagggata ccgtaaaaaa
    15361 gaggaatcat tctggattag atatattgtt gaaaattatt ttaaagaggc aaaatatcgc
    15421 tatctggtac aggcgctgga aaaccgttac ctcatgtata atacattgct tgatgaatta
    15481 gagcataaac acaaaaaaat aagcatcatt tatccaccag atgatttcac tgtagacaga
    15541 ttaacccgcg accataaaaa aattctacag ggatttgaac agggtgtaca ggcaggcaaa
    15601 aattttttac tatcgtcatc gttgtaatac tattattgct cctgaacctt ttttacttcc
    15661 tctttaattt gttcaatact ttgtttatgt ttacccttta tttcctcata acttttacga
    15721 tatgcttctt ttactgccat tattgcttca taatttgctt tttgttgctg taatacccta
    15781 tccgcctgtg attttatttt atcaaaagca tcatatatct cttgtttctt atctttataa
    15841 ttttgtaaaa cagtttcaac tgcagcactt gcacaatcac ctaaaaaata atcatcagca
    15901 atcattgcag ctttttcttc cacttcctgc atatcttgtt ctatcttatc tgaataattt
    15961 tttttaattg gcatcttctt taattttgca atttctgctg attgggttga acttcgttct
    16021 tcaagcgtct gtaaatattc ttcataactg tcagcatact tttgcggctc tattgtaaaa
    16081 tttttatctt cagtattttt ccatgcctcc ggatttttga ggttttcatc tgcttcagct
    16141 ttttttgcaa tctcagcatt tgcctgttcg tccccctgaa tggtgagcgc cccatatgga
    16201 ttaatgagtt ccaacgaccc ttcttccata ttaattctgg aatttgaact atcgctaaca
    16261 gctacaaaaa actcggtacc tctgattgca cataccgttg ttggagtata tatctttttt
    16321 gattcatttt tttgcaattt agcaatcttt gcctttacca caccattcac tactgatata
    16381 ttttcttcct tttcctgtac gcgtgcaatt ttcacctttg cattttcaaa aatttttact
    16441 gcactcccat caagaaaaag caactcaaca atgcttgcct tctttgttat gatagtatct
    16501 cctgtatcta cattcatacc aacggttgct gttagttgct taccattgcg ctctacaaat
    16561 gcctcgccaa ccacaaacga tacctgtaaa ccatacgcat tgtgtatcag caataacaca
    16621 cacatcaatg gcatcataaa tattgtaaaa acatttatca tttttctcat ggtattctct
    16681 ccaaaaataa atttatgtga ctatgtcatg ctggattcag gtctgtcacc ataacaggct
    16741 cgtttcagta tctcttattc aataaagatc ctgaatcagt attaaggatc tatcctttga
    16801 acagttagta cactacaaca tatcaatcgt tgctaaaaac tctattgatc atattccact
    16861 acaatatgca tgccactatc accattgcaa aacatggtgg tatcatttaa ttatttacaa
    16921 tacttttcat aaaaagtgca atttatttat cgtgttgcta cggttatagt taatactatg
    16981 gttcatattt catccccagc attgttacat catcaacaaa gcgatcataa ccacaaaatt
    17041 cttgcactct tttaaacaaa aattcaataa gttcattgat ttccatttta ctatgttcat
    17101 caattaaccg ctgaaactgc ttttccccta atattctgtc aaccttatca gggcattcaa
    17161 aggcaccatc tgaaaaaaat aaaagcttat caccagctaa tagtttaacg ctttttgcgg
    17221 tatacaacga tgtatcaaaa atgccaacaa aaggtccagt ggtttccaga cgtatagcat
    17281 cgccattgtt atggataaca attggtgccg gcaaacctgc attaatatac tcaatgcgat
    17341 gattatccag attaatatgt gctaaaaata tagcagcata attgtagctc attgtttttt
    17401 gcgtctgaat taaaaactga ttcatttcat tcatgatttc ggatggagat ttctcatggg
    17461 taacaataga tgaaatcgcc gttttaacaa tggcagaata taacgctgca ggcacaccat
    17521 gcccggaaac atcagcaaag ataaatgaaa aatgttggtc atcatgaata ataaaatcaa
    17581 aaaaatcgcc actgatcttt tcaaccggtt taaattttaa ataaaaagaa aatcctttta
    17641 tttcagggaa agaaataggg aataaacact gttgaaggtt acttgcaata tcaagttcat
    17701 tttcaatctg tactgacctt gcctgcaact gtgcatagag tttttctaat tcattgtttt
    17761 ttaatttcag ctctcccaca aggttaatat tttcttttat gagtttataa cgttcatatg
    17821 cccgttttac agccagtgaa atctggtcat ttttaaacgg ttttaaaata aaatcaaaag
    17881 ctcctgcctt gagtgcagaa atagctattt caatatcatt ataggctgta attataatga
    17941 taggaatact ttcatttatt tcatgtacct tttgtataaa ttccaatcca ttgatgccgg
    18001 gtaaattgac atcggtaact atcacatcag cctgcattat cacatcactt cgcaatgcat
    18061 cctcagcagt ataatagtta tatactctat attcatcatc aagaataaat tttaaaactt
    18121 cattaaccat ttcatcatca tcaataatga ctacttcagg tttttcaaca gcattcattt
    18181 agtcagcacc cttgtacaga taattcattg aactgctata aaaacgaatt cccacccagt
    18241 ttgcaggatg tttgtaccgt ttatcccgta ttacataaac aaatgcatct gcataagcag
    18301 taaatgatgg tttactttta agagcagtta aaaattctcg taaaaacaac aatcggttta
    18361 catcaataac agcgcaattg aatgtaataa atccacctat ccctttttgc atcaatacca
    18421 atcctgtatt atatgattgc ccctgcatga tagtcaaatc aattattaag catcctggtt
    18481 ttaccagagc atcgtccata acaaaatttt ttttattatc actataccac aatggcttat
    18541 cagtaattat tatacttgat gagatggcaa atccatagtg aaccccagat tgttttacag
    18601 cagcaataat gctatcccgg ttactatcgc ctatatttat tgcaataacc gaagggataa
    18661 cccgcgtgct acccattgcc gctacaagag aaggcataaa ggctaccaca cttcccgact
    18721 gatggtacag aattgcttca aacggtattg catcggtata tacatcggca aagataataa
    18781 cacggctgta tggagcacat tcttttatta caggtttcat cttttcatca atgttatttt
    18841 caatcccccc aacaggctgc aacaatccaa gattgttata gtactcataa accagatcct
    18901 tcacttcact gtaacaattg ctcaatcgta ctggctgaat ataggtccgt gtaataatcc
    18961 acgcaaagat atcattttca ttttttgctg tatataaaag tgctacatcc tttggcaatt
    19021 ttttttgaat cgctgtgatg gggattgcat tccttaccag gttgtcatac tgttgcttaa
    19081 aatattcatt attactcagt actgctatgc catatatcat cctgtgtaat tcggcatatt
    19141 gcaaggcatc tttttgattg cctctgctca ttgaatagga taccatgtaa ttaaaaagtt
    19201 tatccagcaa atagggatag tcgctataat aaaaacctgc catatcactg ctgagcgaaa
    19261 caatgaacct tgcaatagca ttttcagcag ctttgaaaac ggattcatcg gtttttttgg
    19321 ctatagccat cttttgaagc tgaatgttca tcaaaaaaag atcatacagc gatttatccc
    19381 atgatatcat cccgctttca tcaagcagtt tccctgcttc ttttgttttc cccatagcaa
    19441 taacagaata caggtaacgc atataccact gacgttttat ttgaaaaggt agatcatgga
    19501 atgccgtgca cagtgtatgc gcctgcttaa attttcccgt tttttgatat gcaacgatgt
    19561 gctgtaagac aaaagtccat gctggtttgc ctttttgtga agggatgctt tctatgagtt
    19621 gtatggatcg ttgcggcaca cttttattac aattatctgc aatctttatg gtataaaact
    19681 gtatatcaat ccactgagca tactgcaatg cattttgagc agccataagc atcttgagcc
    19741 atgcctcttc atttatatca gcatgcaaca caatttcatg tattacaaac aaggacaacg
    19801 catcgtaatc catgctggtg agggtatcaa cagagtattt attattcttt aatgagttta
    19861 tgagcatacc tgcgttggtc gctatagggt aactatcgat tgcatagcca agcgaataga
    19921 ccaaacaaag ttcacgctgt gaaaggatat aatccgctgt aaactgttgt agcgcctttc
    19981 ctgccttatc atcctcttta aggagagcag caaattgtat atataataac cgtaattgat
    20041 tgagtggtaa cagtgatttt tcatgattat caaaggtatc atattctgca aggggttgat
    20101 tgccattcca tgcttcaata agcgcagcat acacctcatt atcaagagtc ccctgcaatg
    20161 ccgcattccc tttcaaagct tttaaagctt cctccatgtg accctgtagc agatatgcat
    20221 acacaaggta tgaccgggca attgaatcgt tatctgcaat caactgtatt aatgccactg
    20281 catcatctat ttttccttga tagagcagca ccttgatctt atgtatagta tattcattat
    20341 catgcagtcc tgtttgtttc caccgctcta atgccagtaa agcctttgtg taatccccct
    20401 taccaagagc attattgaac aattgactgc tggattttgc aacaaccagc gcattgctct
    20461 ccctctgctt tatatcagat ggagttttac ccaatagcag taatggtgta ttgacttcat
    20521 ggagaaggtt agcatcatcg ctgctgattg ctcttacaat agtttctacc ctgccaccag
    20581 cataatatcc aataatagta gccacatcag catagagtgc cgcgtcactt aaaagtaata
    20641 aatcgttaag cgatagtccc tgaatatttt taacaacagc agaacactct ataacagtcg
    20701 taaaacaata ccgggctgtt acagcataga gcttgttttc agaatcgaca atatactctg
    20761 cttcatggat gtcgttaata acatttggta tacctgacaa tcgcatatcc gttgctatgg
    20821 atgtcactgg cagccatact tttttttcta cttcaggtgc ccgtgccgca catggtatac
    20881 atccaagctt atacaacagg gtagtatcaa gcaatggatg tatagtcata aacgaatcat
    20941 ttaaaatgac atagccccgg ttaccatagt tttgtaaaac agcttcaata tcagcggtta
    21001 tagtttttgc atttatttca tcagatatta atctccaatc aaccgtattt ttatatgcta
    21061 tccagcaata caactttccg ttatagtcat aaaaatgcaa cacaggcaca tcataccgtg
    21121 tcgctctata agcaccaaca tagggtgcca tgaaatctgt agcattttta cggaatgtat
    21181 ctatcttttt atactgcatg gatagatttt cctttattgt atcaatgagt tccttttttc
    21241 catcagctac tatcttctgc gttaatgccc tttcaagctt ataaacagta aaacatgttt
    21301 ttttgtaatg agtaaaaaat tcatcatcac ctttattgtg aaaatcattg agcgttgctc
    21361 ctgacattgc taaagcaaca atctgtttgc gttgctgtgc agtaaatgcc tctctgtatt
    21421 ttcctgcatt gagcaagtaa tccgtatacc tatcataaag attatagata aagcctgcat
    21481 atcgcgcata taattgaggc aacaactcaa cacgcgataa aacaccagct ataaggctgt
    21541 caggattttc aatatgctgc attaaactat tatagcgatt agcataatcc catacagtag
    21601 cttctgcaaa ccataccaat tcttttgact gtacagcgtt tgccttgaat cgtgcgctta
    21661 taatatcatc atagccttcc ttaaccttgc cggtagcaat attgcatata cctctattga
    21721 taagtaattt tatgacaaat tcctgatgat atggatcatt agcgttatag tcaattgcct
    21781 gtgataatag cgctgatgct tgtgaatata aagaagctgt tgcagtgctt tgtcccaggg
    21841 atgactggtt attggccata attgttgctt tttgcatcag caacagagca taaagagcat
    21901 taaatgatat ggtactgcct tcaacctgtt ttgttgcctg ctgcgataat ttttctatat
    21961 cctcagggac aggccttttt ttctgggctt tcatagtatt gatatagtta ttgctaagtt
    22021 gagcataaac acgttcatac atgcttgttg tatagtgctc tatggtgtcc aacgctacat
    22081 cagtttggtg cagagcatcc tttgctccat aactgcctgc agtataacag taagcccatt
    22141 gcagcatcat ccggtatacc ttatcaacag caatggattc tttctgagct attgccagcg
    22201 cttgttcata gtagcgtaca gcatcagcaa tttgtccgga ttgagcagcg caatagccgg
    22261 cattttgcag tgcagaaaat gtaacatcaa ggaacaggct gcctttgcga ttacctgcta
    22321 actttataat tttatcatac cgtgtcagag catcggtaaa cctgccattg cttatataaa
    22381 tctctgcagt catagaatga ataagcaatt gcatttcaaa agatgaaagc tcagaaacaa
    22441 tttttccttc accaatgata atatcatcct gatttacagt aaacacatat acaggaccaa
    22501 gtgttactat ttttaatcct attttgtatt gcttttcttt tgttagtgat acattttttt
    22561 cagcaagttc cagataagtt aatgcttctt tatcgttccc cagtttacgg tagcattcag
    22621 ctattgctat atataatcgt gagttacttt cctcatgatt atagcgtgcc gcatgtataa
    22681 cacatgtagt ataccagtct atcgccttag tataattccc ctgcatctgt tgaaataagg
    22741 caaaatattt atataatgta tagagctgat cttttactga aagctcattc ttctgagcta
    22801 tcgccttata aatagtatat gcacgctgca ttgctgcata cgcaccgcta tagtttccct
    22861 gttgccataa acaatatcca taatggtaat aaaactgtgc ctcttctttt aatgaggtaa
    22921 atgctttttg atagctgaca gccttgttat aatgcggcag ggctaaagta taatttttca
    22981 gcagaaaata tatatttgcc agattggtat gtatatccgc ctctttacgg ggtgttataa
    23041 tttcatcgtt tgcctccaga gctttctcgt acagtggaat gcattgttcc cagaggtact
    23101 cactaaaata tgaatcaatc aaacgcttct tagaagggca acacatccgc aattcatcaa
    23161 catactgata tacccaccct tttaatatat acgcatcaac aaacctatca tcaataaaca
    23221 aagcccagtc aatgtggtat agtgcctgtt tcaacatagc aagctgttta accaattcac
    23281 ctgatgttct tccttccatt gcatggggtg acgagccaag tgtcagagct tcttgcacat
    23341 agatatagcc aagtgcatag atatgcgcct tatcaaaatc catacgcgca accgaaagac
    23401 ggtgaccctg cccggaatat tcatctttaa gagtatccag catggtattc tgtaatggaa
    23461 tactggcatt gatatatcca atatgcgctc ctgcagcgtt tatgttgtat acatcctgat
    23521 agcgacgcct tagataaagc aatgtattca tggtaatgta gcgtttgtac aattcaccgg
    23581 actctttata ttttccttcc tcatacagtt tttgtccgtt tgtttcaaag tactctgtca
    23641 gttgctcaat aataggacgg atatcctgtt tccactggac ctgaaagtta ttagttgcag
    23701 cctcatagta gccagcccaa tcatcactct tctgtttttg tgcaagctca cctgatttta
    23761 cattgattag aaaatatgcg acatccaatt tttttgtatt tgcaagcccc tgttttaagt
    23821 agagccatgc ctcttcattt tgtcccagct catcaaggct tgtggcagct atgtattgaa
    23881 aaattgcctt tgcaataggc agggtatacc gttcaacagc ttccttagca cctgcatacc
    23941 gctgtgctgc cggtaattgt tttgtagcat taataacttc catcgtaatt tcatttttaa
    24001 gataggtgta cgatgagcct aacaccacta tccactcttc aggtatagta aatcccccgt
    24061 acagcaaccg gccaatctta aagcgcacat acgcaagcct ggaataggtg gcataggtat
    24121 caacaatatc acggtacgtc gttaaagctc tctgtgtaaa gccctgagct gcatacctat
    24181 cagctaattc ttcaatgaaa aaaggcgtta gtttttcatt gttttctaac ttcaactctt
    24241 gtatattttt ttcaaggtat gttattgtat tgttttcttt taatagtata aattgcgcat
    24301 acaatgatgc cggcttgctt ttgggcgata gttcctgtaa tatctgtttt gcaattgtat
    24361 agtgttcatc ccttgaaagc tcatacaggg cctttgcaat aatcatctgg gaatcaatat
    24421 ccaccgcctc tctaaaaaaa taatatgttg caaacaaaca tcgctgatat cgttctatat
    24481 catcaaaatc ttctttatat ttcagtgcca gttcatactg tctaagtgca tttttttgtt
    24541 ttggtattat ccccgtatgg ggaatcattg ccatgtttat gtgttcacct tcaaccacac
    24601 tatacacaat aaccccgtca gcatatggca cccatcgtgg cgcagttgaa tattcatcaa
    24661 tcaatgttaa cggatattct tcacccgtta ttacattgta ataatacagc ataccgttat
    24721 cactggcatc aaccactcca tcactgttag tatcatgctc aacccgtgta aaatagatat
    24781 catcatctgt ttttccatag cacggtgata gttgcagtga cgaattattg gtgagctttt
    24841 gtatagcacc ggttgcgatg gttatccggt aaatgttggt ttccttccca tcctgttttg
    24901 taaaaagaat cgcattgcca tcatttgaaa accgtggttg catgccactg gttatcttgc
    24961 gaactttatt accatccctg ctcataataa aaatactttc ctcaccttcg cgctcagatg
    25021 aaaaggcaat ccatttacca tcaggcgaaa agcatgggct ggcgtctcgt atggggacta
    25081 tagcgccggt tgctgtatcc tttattgttg taagattttg tgcaggttgc tcgccttcta
    25141 atttgctctg tgatagtatc gtgtctatac ttaatttcat gagcaaaata tcaccctggg
    25201 ggtcgtcccg tgttgaaaca aatacacacc atttaccatc aggtgaaaca tcaggggcaa
    25261 aatcttttgc agggtgcact gttaaacgaa acgttgtgat gtcattcagt ggtctaagat
    25321 atatatcaaa attattgcgt tctctgtttg atgcatagat aaagtacttc ccatcagtag
    25381 taatagtacc acttacattt accgaacgct caaaggtaag aggtataact ctgccagtac
    25441 caaaacgata ctcttttgtt aaaacctgtg aataatcaaa tggcacatca cgctgtgcag
    25501 ttgaaacaca tccagcaaac gatagcaaga gtaacagtac tatgaacaat aaacgcttca
    25561 tttcttcacc cagcggccgt cattgttttg tatccagtca ccttttttag cttttgcatg
    25621 ctgctctttt gcaaattcaa ctgcaaactg tatggctttt gcttcactgc ttctggcagc
    25681 accagaactt ttaacaaaga ttacactgcg ggcattattt tcctgttgta caacggtgcg
    25741 tattacatca gcctcttgta gtgactgaag ctttttttca tcaactattg caatatatcc
    25801 attattctgt tcgccaataa gctctttatc cttgtataaa cgtattgtat cgctgtttaa
    25861 ttctcttatc tggatggcat tcaacaactc atcatcagca ttgacaaccg ctttacgatt
    25921 cccttttgat ggggtcatca ccgttgatgt aacccatgcg tcctgttcaa gctcatggta
    25981 ttctccaatg agctgtcgct ccaatgcggt cttttcaccg gtaatattga ttgcaggggg
    26041 gtatatctca aggcaacttg gcatatcttg aatgcagcct gaggaagtac atccagacat
    26101 aacaataact atacaaacaa tacacgcgag tgctatcgcc accatgtttt ttttcataat
    26161 gattcctccc gcaccttgtt gaaaaactcc atcaccggaa tgcgttcata gcggacccgt
    26221 tcattgttaa ccgtcaccag tgcacccagt attttgcgct taaataccac cgttggatat
    26281 acaagcccct tatcaatata aaaatcaaag ccagcaatga taagcgaatt atccacagca
    26341 atttgtgcca tgcccaattt tgatttcccc ttttcttcat tcaagccttt gaacaatcgg
    26401 ttggcaaact gttcaccaac cttgtaaata gtagcagtac ctgaaatgtc aagattctga
    26461 ccggtagata aattctttcc ctgcattata gcattcatgg ataacaaaga gtcgtgatgt
    26521 attcgcaggg aagatggatc atccagtttt ccaatatcaa tatcattgac attaaacgca
    26581 actctaaatc ccatattctc cggtttaaga tctgccagat taaagagtaa ctcctttaat
    26641 gttaccgttc cccccattac tgttgcctgt acattcttta tttccagtgt gttgcgttta
    26701 catccaatat accctttgca actactcata tattcaaatg caatattacg cgatggatgt
    26761 tttgctacaa tcctgcctat agaaacattg acctcgggtt taaacagtgc aatgttcata
    26821 ctgtcggcaa catcaaacgg tatctcaaag ctctccccca tggtatacat tattgggata
    26881 tcaatatcaa cagggcctac ataaagttta ttgggtttac tttcaatctg tagtttcttt
    26941 gatacaatat taccctttgc tgataatttc tgcactgttc ctttgagatg cattgcaact
    27001 gccagttgac cactcaaaga ataatcagca aatgcaacgg gctttgcagg tgcaacagtt
    27061 aatcccgtac taacatcaat catgatatca ttccccatga caacattgcc atgagcctgt
    27121 atggccatat tttttttgct actggatagc tctaaaacag agatggtaat tttttgctgc
    27181 tcaggtttat gagctattga cgctgtaagt tttagatcat caatgtcata atcaggtatt
    27241 gcaaatatgg tagcaagctc tatcgcattt acttttttag aagattgcaa cgtggtgttc
    27301 aacgtaacac taatcggttt tgttatagga cttgatgata gtgcttgctt caggtgagca
    27361 ggcaatgtat gtgttaatac cggcatatta atcatcaacg gatgtatggc aatgcgcgcc
    27421 tgtatagtat catgcaatga aacggtacca tccatctcgc accgtattgc ttcttttgca
    27481 ctagcagcat ctcggtgtac catgacaaaa cgatgtattt gtacaaaacc cgtgttgcga
    27541 ttgtaattga tatcacatac cagatctgca ctattatcct ttgatttttc accaagtgca
    27601 taataaaagt gcggtatatg tatccgtagc gatcctgtaa cataagcgtt gctcatagca
    27661 acagtgtagg gtatggtaaa tgaagggacc gttattgaaa aatccccggt agcaactgct
    27721 acattttccc cgctgatagc acccgtaacg gtgaaattat tccccttacc tgttatcata
    27781 agaggaaata gggagacagt tccatgaaag tatgggctgg gccctatcag cttgctggta
    27841 taggtataca actcgtgaag cctgatattg ctatcaagca tcgcaaagga tatctcagga
    27901 cttttgttaa catgcagtat actgccagca aacgaaaccc aggtggattg attaaatgta
    27961 atcataaagg tattacagcg aagtacgtca gttgaaggat tatagatgat gtcatattca
    28021 attttagcat ttaacggtgc tacatgtttt tgaacaaaac gcagcggcgt agtatacgtg
    28081 ccacagatga agttgcttga tagttgcttt tgatcagggt tgtacgcaac atgaaatgaa
    28141 aacaacaatg gtggacctgc ctttacatct tttgatataa atgaaagctg tagtgttttt
    28201 tgtggattta tcgcaatgtc cattgtatca aacaaggcta acgcctttac attcaatggt
    28261 atggatctca ttgaaggtgc atgtactgtt atatatgatg aaaaatcatg acatcgtagt
    28321 tcataggtac tgccatgtac ggttattgaa agattttgta ctatgaagtt aaaaagaaat
    28381 tccactggaa ttggcaatga aattgcttcc cctgcttcaa ctttttgctt ttcttctttt
    28441 tcttcttttt cctttttttc agagggtttc agcatcctct gaaaattcca tacccctttg
    28501 tgttcatcaa tgacaataga cggattatac agcccaacct catagatgtt aaccttccca
    28561 ataagcattg gtaacaggct atactttagt ttaaaggtat caaactgtag taatggtgtg
    28621 gtatcaccca aaacaaaaag tttacaattt tttatttcaa atccatgaaa taatgaaaaa
    28681 tgggttacct gtaatgaagc tgtagcgtta aggctggatt ctatctgtga gatgaggaat
    28741 ctggttgctt tgctatcggt gatataaatg ttaacaccaa taataccaat gaacaacaaa
    28801 actatgaaac cagcaacacc aatacttaaa taatatgcta cctttttcat aagatggacc
    28861 atgattgcac ggctatcctt tcacacataa aatagtgtaa ctacggtcaa taatctctat
    28921 actgttttca tgcactatag tgcagtataa gtaataccca ttaaaattta aaaatatagt
    28981 attacaacta aatatttata gaaactatct tttttcattg acaactatct atttcaaagg
    29041 ttatcatgac attaatttat aaaaatgtaa agggtttttg tatgagcaca ttaaaagatt
    29101 tattttcctt agaatacgat aaggatacaa aaatattatt aggaacaatc aaaaaaccaa
    29161 tccttaccaa agaagaggct aacgaaatct tagaatttgt caagcaagat atgcaaaact
    29221 accctgacca tcagcactat attctcaatg tattaacagt gaaagaggta tccaacccta
    29281 taataggtgt tttaatgaaa tcgttgcaat tctggaagaa aactcgcgga tatgccctcc
    29341 ttgtcataac cgatgacctt ttacaaaaaa taatgataga acagcctgca atgtttgatt
    29401 tctttgccgt tttcccaact gtggacgagg caatagcatt tacaaaaaaa tcttagtgct
    29461 acagctcttt aaccagcttt ctatcgaccg ctacattttc catgcccggg taatccacta
    29521 tagcagcttc tgcagtttgc tttttaaatc gcataacatt gagcataaat atatctaatt
    29581 tattcagtgt tgtaggaggc aatgcattgc catcaatctc cactgacatc atgggatatc
    29641 cttttgtacg tgcccatgga gtaagtatgc cttcaatgac ccttcctatc aaacatgcaa
    29701 atggtgagat attcacaata cctgaatagc catcctccat tgcgcttgca gcaacaccgc
    29761 ttgaaatagt aatctctgac tgcagttcaa gattaacaaa atgctgctgt gacatcttca
    29821 taatgtgatg catgttatga ggtgtttcag gaatcaggct ggtaggcatc aaagcttcct
    29881 ttatcttgtt ttcaacacca tgtttgtacc actgctcaat gtaaaacaat agcaatttct
    29941 ttgtttcagg tataaatagt tttttatacc acggcgcggc cttgattgct tttttatact
    30001 catattcccg gataaaatca caatagtaaa tccattcact gacgcccgaa acttttgcaa
    30061 tgataccttt tgtgctgaaa tgcttgatca actcatcaac agcaaagtca tctcgcctaa
    30121 cataaatttc accaacaacc agtaccctgg gacattgttc gatagttttc tttaatggta
    30181 tttgttgtat ggcagcagct atcctttgta aagcaggtat aacttttgtc acatccttct
    30241 cggcaatcga tatcaactcc tgccacaact ggtcgtacaa tgccattgca cttaccggat
    30301 cttcagcgca tgctcttagc gaagtttcaa tatctttcat gtagtcgctt ataacaacac
    30361 cccaccatgc atgttttgaa aaatgtgatc caagctcatt atacgaattt gaagaatcca
    30421 atgtaataac acacacattt tccaatcgta aatccttaaa aagattttga taaaaaacaa
    30481 aatattgccc cgtacggcat gggccctgtg ttgtaggaac aaacaaaaga tatatttcat
    30541 ctttccggta gtgtgtggaa gaaaaaaagt tcaatgcact gcccagaacc agatgcgaag
    30601 gtaaacattc tttgcctgat acaaactgcc gtgctaactg caatgttctg ttatcaggca
    30661 caggcattgc tactgcattg ataccggttg aacgcagcga ggcagcaaga agctcaaccc
    30721 ccaggcgtcc catgttagat aaaagaacct ttaccttcgg attgttgcgt ataggtattt
    30781 tactgccggt atagctattc tttacaaaaa gaccattatt ttcaataatg aaacgcaagc
    30841 cgttgtcata gcgttctgcc tgtttatcaa tacatttctg ccggtatcca tcaacaatat
    30901 caaggaatgc ttctacacgc gtatcaacgc cggcatcagc agtatgtgaa tctaattcca
    30961 gaataagaaa tggctttgtc cccattatcc attttacata gtgcagcatg aatgaatcgg
    31021 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncccagcagc gctatcgcag
    31141 gttttgacaa cctctttgaa aactcaagcg cctctttccc taatttttga gcttttacaa
    31201 aatattcttt ttgcttatcc agagcaacat gataggcact catcccctgc tcagcagaaa
    31261 atcccagtgt cacggcaaca tcaacaaagg attgttctgt cattgcatct gaaagcttaa
    31321 ggcttataac cggtgaaaga attttttctt ctggcaattc atcaaacgcc ttttctatat
    31381 agtatggcaa tccctgcgtg attggacaaa atgttgcccg tgcatcatca acaaaacttt
    31441 tcatctcttt catgtgtggc agaaaaatat aatcaacacc tttatcaagg atatttttta
    31501 cagccccgtg ggctatttca gccgggaagc aatatgcact ttcagcttta gctttccctt
    31561 catctgaaat ttcatccgaa gtaactatcg ccacacctaa ttgtgaaaaa aaccatgcat
    31621 acaatggcca caacgagtag gttgaaaagc aaagaggtat cccaacacga ataggagaac
    31681 tgcccgtcaa tggctgcggt gcgcattctt caaataacag ttgattccgt ttttcaataa
    31741 agtcaacaac ctcatcatca ttgatgatac gctttttacg catattggca tatttattgc
    31801 atcgcccacc aaacatatat ttgttgccat tgactttcag cacccgtatt gggcaatagt
    31861 tgtcacatga tttgcaggta aattcgcgtt cataaataat ttcagtttgt actattgcat
    31921 caagcgaata tagcttcttt tgcaataatc cactctgcag cttttgcaac gcaaccatag
    31981 caacaccaaa acaccccatg agctccggat caggcggtac ggttatattt ttttgcaaca
    32041 gcatggcaaa cgcaagcggt actgctttgt tctttgccac tccaccctgt agcacaatgg
    32101 cattaccaat agttctgttg ccaacaaccc tgttgagata atttgcaaca attgatgtaa
    32161 ccaatccagc ggtgatatcc tccttgcttg caccttgttg tattgccttc cggatatctg
    32221 agtttataaa agctgagcag tgttcaccaa acttcagcgg ctcttccgcc tgcaaagcaa
    32281 tatcccctat ttcccatgca tgggcaatat tcaggtcacc ctgtgctgac tcctcaagga
    32341 atgaacctgt ccccgcagag catgcctcat tcatggcata atcaataggc actccgtttt
    32401 tcaaatatac atattttgca tcctgcccgc caatctcaaa gattgtatca atatctggtt
    32461 taaaataggt ggtgccaacg gaatgagcaa ttatttcatt atagattgcc ggtgtttcta
    32521 aaaaaacgcc cagtatctca cgtgacgaac cggttgttgc tgcaagcgat atggtaatag
    32581 aagcatctcc tatctgctca tataattgct gttgaatctg ttcaatgcac tgctttaacg
    32641 cttttacagg gtctccatgt gttctgccat aataatcagc aactatctca tgggtatgta
    32701 tatcaatgag acatgctttg gttgttgttg aacctccatc aacacccaga atatattcgc
    32761 caccgggttt tgccctgctc cgttttgatg ggatataggt tactttatcc agagcactgg
    32821 ttaagttgct aaagcgtggg aagctgacgg tatgtttttc aaatagattg tcgccaatta
    32881 accccccgta tgtttgcgca agcaatgcag caccgtaggc ttcaaaatag ggagctgatt
    32941 caggaaccaa aaaggtaaca ttggggagct tttctttaat aaattgcagt atgaatggat
    33001 tttgtgtaat accaccggtc agaacaacca cgcccttttg tatacgtgcc ttctttaaaa
    33061 aatcaacaac cttgatagcc ataacattgg acagtgacag tacaatatca cctttggtgg
    33121 cttccccttt attgagcctg tgggtacagt cgcttttcat aaataccgag cagcgcgttg
    33181 ataatttcat caccttgcta tctgccggga tggatgcaac atcgttcagc gtcatatcca
    33241 tacgcgctaa ctgctgttta aaaaattcac ctgtccctgc agcgcattta ttaccggaga
    33301 agctggtctt gattttttta tcttcaccga tggtgtaaac aacaagatct tc
//
LOCUS       DAOOJH010000019        32342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1485_length_32342_cov_9.995974, whole genome shotgun sequence.
ACCESSION   DAOOJH010000019 DAOOJH010000000
VERSION     DAOOJH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32342
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1485_length_32342_cov_9.995974"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="rsmH"
                     /locus_tag="PLJ75_04400"
     CDS             <1..479
                     /gene="rsmH"
                     /locus_tag="PLJ75_04400"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPD04488.1"
                     /translation="KYGEERWARRIAKRIIYKRSEQPIETTGQLADIVVQAIPKQYRV
                     KNIHPATRVFQALRIYVNDELSAIGESLKDAITFLKPGGMIIALSFHSLEDRIVKDRF
                     RRWAKGCSCDNDGLHCQCNSGPLVAVLTKKPLQPEESELEKNQRSRSAKLRVCRKL"
     gene            485..793
                     /locus_tag="PLJ75_04405"
     CDS             485..793
                     /locus_tag="PLJ75_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04489.1"
                     /translation="MNTSEQKSIVQYSTIVICVICFIVAFIYQNIEYMQLKMQYDAMV
                     NEYKTVVEEYDRQKYILQKAVSMKNLDAFAQLHGLVPMSKDSVVIVENNSKNTMQEQR
                     "
     gene            790..2319
                     /locus_tag="PLJ75_04410"
     CDS             790..2319
                     /locus_tag="PLJ75_04410"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPD04490.1"
                     /translation="MMEIKIYNSFDLYTLLDSMDVSIIKGDEDIRISSVEYDSRRVEK
                     ESIFVAIEGFETDGHKFIKDALENKASCIVLNENRLDEFSFLLDRNDLAVVTAKDTRK
                     ALSQISARFYGEPSLQAVVIGVTGTNGKTTTTYMLESILSKAGFHCGVIGTINYRWKD
                     KKINALHTTPESKDLHEMMSIMVLDGVDCIIMEVSSHALSLLRVDDIHFDCALFTNLT
                     RDHLDYHITFNDYFMAKVRLFEILNKSCKQKKAAFINSDDNYGRQILNWRQRFNYPLF
                     SYGIQNNADYHCIESSIQTSIRGTRFATDVPFAKQFEMRLAGKFNVYNALAAIGVAHF
                     LNIPAGRIVTGIGLVASVPGRFDIVLSPEGFAVIVDYAHTNDALEKLLLGVRSLNPQR
                     VITVFGCGGNRDKTKRPIMGKVSEEHSDWIIVTSDNPRKENPEDIIKDITAGMKSKKY
                     QIIVNREEAIKQAIAMAESGDVIVIAGKGHEDYQIIGTKKIHFDDKEVALKYINERNA
                     K"
     gene            2316..3686
                     /gene="murF"
                     /locus_tag="PLJ75_04415"
     CDS             2316..3686
                     /gene="murF"
                     /locus_tag="PLJ75_04415"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPD04491.1"
                     /translation="MIVQFETSSVTIARAIGAKHYGADMAVKHITTDSRLLDDKSLFV
                     PIAGQKFDGHDFIEEACKSGKCSSVLTHKHTDVAIAERYGVSAIVCDDTLHAYMDIAL
                     WHRKNFDIPTIGITGTNGKTTTKEMIANVLESQGRVLKTEKNYNNEIGVPYTLLHLNK
                     EHAFAVIEMGMNHLGEIHRLSLAAAPDVAVITNIGEGHLEFVGSTMNVAHAKAEILDG
                     MNKGICIINNETKHKDIVINKANDKKIKVITFGLTGDIRPATFAVYADKTVVQYDGID
                     FVVYAYGFHNVYAMLATIAVAKVLGIPLNNVKESLECFKPVSMRSALHNGRCIIIDDS
                     YNANPLSMEYAFASASMVFPHKRKIAVLSDMKELGNVSKELHYEIGAKLAHSGFAYLF
                     IWGNDARFYADGAIAAGFERSQIYIYDDKEELIQALRQFIQADDVVLVKGSRSMKMEE
                     VVKALE"
     gene            3697..4782
                     /gene="mraY"
                     /locus_tag="PLJ75_04420"
     CDS             3697..4782
                     /gene="mraY"
                     /locus_tag="PLJ75_04420"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPD04492.1"
                     /translation="MFYHFILPLHEYLSFLRLFQYITFRSAYAALTSLVIVLLFGGMV
                     IRWLTYLRFKEEIRALGPQSHQIKAGTPTMGGVLVLGSVLISVLLWGNFNNLYVVLLV
                     VVTVLAGALGFADDYIKSILKKKDGIPARLKFFMQCVIACAVVVVIYLFPSNSKEATT
                     LYVPFINKAIIDLSWWWIIFAILVIVGSSNAVNLTDGLDGLAIGTVTIVVGVLAVMSY
                     VSGHQPIAQYLRIPYIPQAAELTVFLAALGGAGLGFLWFNSNPATVFMGDTGSLALGG
                     IIGTVAIMIKKEIFLFIVGGIFVIEALSVIIQVGSYKLRKKRVFKMAPIHHHFELSGV
                     PEQKVVVRMWIVGIILAILGLSSLKIL"
     gene            4802..6178
                     /gene="murD"
                     /locus_tag="PLJ75_04425"
     CDS             4802..6178
                     /gene="murD"
                     /locus_tag="PLJ75_04425"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPD04493.1"
                     /translation="MKTQHILVVGLGYRTGLASANFLVNKGFDVTVCDIKSSEELSPI
                     IQKLDARVKVIAGNQHPSLLENGFDEIVLSPGVPQSIPLIQEAYRKAIPVISEIELAY
                     RYCKAAIVGVTGTDGKSTVTMLIAHCLTQLGFDAHPGGNIGIPFISLVDSLHEDSVAV
                     IELSSFQLETVKTFKPDVAIITNLTPDHLDRYNSLYDYFNAKMNIAINQCKDDYFIFN
                     RDDEFIVNNMPALQSQQHSFSLLGKADCFYSNGAIVMDSNGETTLVAYASNMKIAGLH
                     NVQNAMAAILASTFIGKKFGNNIDLKVLEEALYSFKGLPHRVQNIGEYKGRIFINDSK
                     ATTTGAVIMALKSLQDNVILILGGRAKGDDYSRLKPVIKGKVKALVLIGETKDEFASI
                     FSDIPHIKAFSMEDALIKAMKLSAKGDTILLSPACASFDMYKNYEQRGNHFIEIYSKL
                     VRGELAWM"
     gene            6169..7326
                     /gene="ftsW"
                     /locus_tag="PLJ75_04430"
     CDS             6169..7326
                     /gene="ftsW"
                     /locus_tag="PLJ75_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPD04494.1"
                     /translation="MDVKAVIDTRRKGEPEIALFIVSFILIGIGIAMSYSASAIFAER
                     VFGDSLYFLKRQLVWCCLGFFIMMIVQHIDYRYYQHYTRIMLLFTMIALILVLIPGVG
                     ITVKGSSRWLGYGPLQFQPAELVKLFVVIYFAKVFSYDNHREINVLQLLLPLLVVGIF
                     FLLIMLQPDFGTAINLAIISVVLLFVSGFPLIYMIGLALLSVPMFYLLIYQVEYRRQR
                     ILAFFDPWSDRFGKGYHIIQSFIAFKLGGMLGAGLGSGTQKIKRLPEPHTDFIYAVIA
                     EEAGLLGTFSILILYLFFFYFGVKIALKAPDEYGRLLAMGLTLLITVQAFLNISVVIG
                     LLPTTGIPLPFISYGGSSLLVNMMATGILLNISKYREVVPRDIKFDKEVLV"
     gene            7323..8432
                     /gene="murG"
                     /locus_tag="PLJ75_04435"
     CDS             7323..8432
                     /gene="murG"
                     /locus_tag="PLJ75_04435"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPD04495.1"
                     /translation="MKTPVLISGGGTGGHIMPGIALYEELKSKGYEVFLLVGTGDVKF
                     SSLNSVENIQVYNAPTMWKNIVNFPVFTIRFISAMLWTMKFIAKNNIAAVIGMGGYVS
                     APSLVAAMIKKIPIYLCEQNVVPGMVTNFFAKRARAIFTTFEATTTYFDVESTGKIVH
                     AGNPIRKQVLTPVEKDAALKRFNMIHCKKVILVIGGSQGALKLNELTYDMLVSYPDRF
                     ADVGIIWSTGTYSYQQFKEKLHTIKHASIYLAPYITDVENSYAACDIAISRAGAGVMM
                     ELAAWGIPSILIPYPFAAKDHQKKNAQEFVKAGAAEMINNSDATAQVVATLLFDLLDN
                     ETLLRKMSAKAKSIAKHNAAEIIINYIIKDSDLKK"
     gene            8445..9827
                     /gene="murC"
                     /locus_tag="PLJ75_04440"
     CDS             8445..9827
                     /gene="murC"
                     /locus_tag="PLJ75_04440"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPD04496.1"
                     /translation="MFRKSKKMHFIGIGGIGMSGIAEILINLGYDVSGSDLRESEQTK
                     RLRQLGATIYIGHYPSNIKDYHVVVTSSAISKNNPEIIEAKKRKIPVIHRSEMLAELV
                     RLKHGIGVAGTHGKTTTSSMLAYILYHGGLNPTAVVGGKVLNFGSNARIGEGQYFVFE
                     ADESDGSFLKLLPTIGIVTNIDADHLDHYKYFEGIKEAFITYMNNIPFYGYSVVCMDD
                     PVVAEVLPRIERPYVTYGFNPGANFKATNIKLLNGSTRYSCYYNDKLLGEIVLNLLGK
                     HNIINSLAVVAVALELGLTFGVIAEAIAQFQGVGRRLEKIGETNGIIVVDDYGHHPTE
                     IRATLDALKQLGKRLIVVFQPHRYTRTQYLYDEFGQSFTMADELFLTEIYPAGEEPIE
                     GVSSNLIQQSVKKHEGRDVVVIPHLDQVPDYISPIVREGDVIVTLGAGDIYKVGHAIL
                     SMLDKKGVVQ"
     gene            9824..10513
                     /locus_tag="PLJ75_04445"
     CDS             9824..10513
                     /locus_tag="PLJ75_04445"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPD04497.1"
                     /translation="MKSNLYTASMIVSLLLFPLYGYASVEAISTVKLYGLKYVTKFEI
                     LNNAVISKDDRFFVDMKKLNNSLQNNPMIESYTVDTNNNILTVKVKERDILAIVLIEK
                     GKDTIPIEVDSHLKIISTYRVHAYDRPIIKITQDEVYHNKLSPRIIKILMIMNKLKNV
                     QGQAIGRISVIDCINPFVSYVSIHGLQTRYIWDETYEGISLMYSVPGLLDSKSHCPGN
                     GVVTQKRIVVW"
     gene            10518..11759
                     /gene="ftsA"
                     /locus_tag="PLJ75_04450"
     CDS             10518..11759
                     /gene="ftsA"
                     /locus_tag="PLJ75_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPD04498.1"
                     /translation="MKEDTIMENIVVGLDIGTTKTCAVIGFLNENKQIEVVGVGVAPS
                     KGLKSGVIVNIDNTVASIIKAIDDAELMGGCEVNSVFVGITGQHIKGENSRGVVAVAN
                     RNRTITPVEMKRVIEAAQAIVIPMDREIIHVLSKEFAVDDQTGIKDPIGMSGVRLEAE
                     VHIITGSTTSIQNLVKSVNKAGFVCNDIVFSPLASAESTLSRDEKDLGVALVDIGGGT
                     TDIMVFIEGGVAYSAVLGIGGIHVTNDISIGLRTPIDSAEVIKKKYGCAVVDLVDASE
                     TIEVPSVGGRAPRRLFRHELAQIIEPRVIEIMEMIDNELVKSGKKEILAAGVVLTGGG
                     SMIEGTVDAAERVLNMPVRVGFPENIVGLKDVVSTPMYANGVGLLRYGAKMGQLRQTR
                     KTPGGLKSLKDKLRQIFNDYL"
     gene            11832..12998
                     /gene="ftsZ"
                     /locus_tag="PLJ75_04455"
     CDS             11832..12998
                     /gene="ftsZ"
                     /locus_tag="PLJ75_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD04499.1"
                     /translation="MFKFDEEPQMKTIIKVVGVGGAGNNAVNRMIATGLEGVDYIVVN
                     TDSQQLKASLAQTKIQIGTKLTRGLGAGADPSIGREAALEDRDKLEKGLKGADMVFIT
                     AGMGGGTGTGAAPIVAEVAKESGALVVGVVTKPFRAEGRRRMQHADEGIKNLKEKVDT
                     IIVIPNDLLLKIIDRSTPMDHAFKLADDILRQGVQGIADIIMVTGLVNVDFADVRTVM
                     KETGDAIMGVGIGVGENRAIEATQMAINSPLLEESGIEGAKAVLLNIAGGSDMSLHEV
                     NEITEIINKQVDPDANIIFGATIDPALDDKIRVTVIATGFDRKLNMLNRPNELDRATG
                     TPLTVIEGKNHKERIEKKVPAQMKSFTLDYEKRRVKDYSHEDLDIPAFLRRNAE"
     gene            complement(13009..13944)
                     /locus_tag="PLJ75_04460"
     CDS             complement(13009..13944)
                     /locus_tag="PLJ75_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04500.1"
                     /translation="MNTILIVDHTDYKNILKDYFSSYYSVLFADCAFDAISLLNMHDI
                     DLVISQVALPGDNAFDLYNYLQNHYTYIPAIMITESNIDIFFDEIFRQGIGNVLKIPV
                     NKTEFVQLVDKIISRNNVFGLSNYIHNIIETNSIRINASKQIQSGVDTILATIESWGF
                     TIKNKVVLSLILNEMIINAVYHSHGYTHEKEMRVPIELKEGQYVDISFCHNASTYGIA
                     VTDYQGTLSKGKILESIYKAIEQEQLILHAAKTGEDITDKISETGRGIDLVRKLASEF
                     YFVIQKNKRTEIILLFESSSSIQTSSSLKIIEDMR"
     gene            14431..14901
                     /locus_tag="PLJ75_04465"
     CDS             14431..14901
                     /locus_tag="PLJ75_04465"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD04501.1"
                     /translation="MKKIMMLTLCIVFSLAIISDSSIAQDTVKKGTKEYIADLSSNDE
                     AVVVAAEEWIADNKEKSALSRLQELLRSDNRVKVRLYAAIALGEIADESAAESLNNAL
                     LNDPSADVRYTALLGIARIGSKDSYDAIQKARESEQDPFIKDLIQKMEDKLKGK"
     gene            15067..15852
                     /locus_tag="PLJ75_04470"
     CDS             15067..15852
                     /locus_tag="PLJ75_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPD04502.1"
                     /translation="MEQQVKYVVFDIESIPNAPLIKQVKYPHLSIGEHDAVIKFQEEI
                     LNVSDGSTWFIPVTFQLPVIVSMLEVDEKFFPIRMHTLDEPQFRTEVIVNQFWEAIES
                     IYNDASYVTFNGRGFDFPLMELMAFRFGVTAKRHFKDKFASRFRFGTKHIDIHDWLSN
                     YSAIRMQGGLNLLAKVIDKPGKVEMTGDDVYDLFKEGKLQSIHEYCISDVMDTYFVFL
                     RTRVMIGELSREREGVIIQHTRDFLEKNKSAVKGFEQYLTNWK"
     gene            complement(15927..17588)
                     /gene="ilvD"
                     /locus_tag="PLJ75_04475"
     CDS             complement(15927..17588)
                     /gene="ilvD"
                     /locus_tag="PLJ75_04475"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPD04503.1"
                     /translation="MISDRVKKGIEKAPHRSLFKALGLTDDELSKPLIGVVNSFNEII
                     PGHIHLKNIVEAVKKGVIAAGGTPIEFPTIGVCDGIAMNHIGMKYSLGSRELIADSIE
                     IMAKAHAFDGLVMVPNCDKIVPGMIIAAVKVDIPSIIVSGGPMLPGNYKGKAIGLDEV
                     FEAVGKAAKGLISKEDLYEIECSACSGAGSCAGLYTANSMNCLTEALGLALPYNGTIP
                     AVMADRIRLAKESGKKIVELVKKQLTPKKIVTKKAIENALSVDMAIGGSSNTALHIPA
                     ISHYAGIPIRLKDIQQISERIPHLCSIAPAGPHHMVDLHYAGGMPALINELLKHGLIH
                     GNAMTVTGKSIAQNVKDKTIFDTSVIRNVTKPVHKDGGLAVLTGNLAPDGCIVKQAAV
                     DPSMYKHSGPAKVFDSEEEAVKAIMGGQIQKGDVIVIRYEGPRGGPGMREMLTPTSVI
                     AGMGMDKEVALITDGRFSGATRGSSIGHISPEAASGGPIAIVKDGDIIEIDIPAKKLT
                     LKLDDKEIKARLAKWKAPKPKITSGYMYRYSLMVSSADRGAVFES"
     assembly_gap    17618..17717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17794..18420)
                     /locus_tag="PLJ75_04480"
     CDS             complement(17794..18420)
                     /locus_tag="PLJ75_04480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD04504.1"
                     /translation="MALRRVISVGASEDRLEKLIQKRLKPDVNKELIDKRIWDLFGEV
                     WCVMFTDLAAFSRKVATYGIIHFLQTIYESQRLLVPIIEEYDGILLKTEGDSMLVIFR
                     NIVKGLECCKTMHQVLYDYNNNKHEEDHIVLCVGLGFGTMLRIGDEDVYGAEVNAASK
                     LGEDIAEAWEVLVTNSVKENANNISNITFEPIDFIPPGAQSAYKVIFK"
     gene            18881..19888
                     /locus_tag="PLJ75_04485"
     CDS             18881..19888
                     /locus_tag="PLJ75_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPD04505.1"
                     /translation="MFNPLKIFKNKKVGLALGSGGAKGIAHVAVIDYCEKNAIPINII
                     AGSSIGAVIAALYAYGSLKEFVSFLRLVKSNDIFSYFDPVFPKTGLIQGRRMIELLAK
                     FIPKSVSIEDLKIPVAIVATDFYSGNPVVFKKGNVLEAIRASISIPGIFVPVRYMDTV
                     LLDGGVANPLPIDVVKQMGAGITIAVNLHPTIKVKKAKIAAMNSLQELSPKEENSSND
                     IIEHVDKEPVDTLSKITQRSGGWINKAKQWLGMNSEGNAAKDNIPNIFEIVSGTIDIM
                     EFMNTTLMITYNKPDVLIEPDLLQVSTLDFTKVDMLLQEGHAACERVQKDIKHKVLRW
                     V"
     gene            19892..20473
                     /locus_tag="PLJ75_04490"
                     /pseudo
     CDS             19892..20473
                     /locus_tag="PLJ75_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            20821..21945
                     /locus_tag="PLJ75_04495"
     CDS             20821..21945
                     /locus_tag="PLJ75_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04506.1"
                     /translation="MSTIEVKEVKDSKMMKQFIMFPWTSKIYENDPAWVPPIIGDMKK
                     LFNPKKGYFYDIGEVAYFLAYKDGVPAGRITAHVNRMYEEKYNKETGFFGFYEAIDDL
                     DVARALFAVAEGWLKKKGKTRVEGPQSFSIYDSVGFEVFGEEIMPVVGLLHFAPYYKD
                     HATACGYTKCIDWHCFLVKKIDDFKPYLKDVRENIMKNQDVKYVYYTPKELKKRAKEI
                     HEIFNRAWDGNWGHLPLTDKQFKMLVDELKLVAVPELIVFAEKDGRTIGFIVSLPDMN
                     PALKILNGRLYPWRLIKALRSIKKVKRIRTIIMGVLPEYRGQKIDDVFYLFTIENGIR
                     LGYVESDCSLIVETNQKMIGALKPLKGQIYKTYRIYEKHI"
     gene            21942..22184
                     /locus_tag="PLJ75_04500"
     CDS             21942..22184
                     /locus_tag="PLJ75_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04507.1"
                     /translation="MIKNTKRLQQFEKEFVQKNSLSHLQALKLLDGMWEMGMTLGVLP
                     PDDPWEGIEVDIRVARIVNCFKEEISFGMYATLKSW"
     gene            22390..22605
                     /locus_tag="PLJ75_04505"
     CDS             22390..22605
                     /locus_tag="PLJ75_04505"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HPD04508.1"
                     /translation="MNKQKLLKPVNVMMAIDFVCLSGTAMLDDAIPKDIYGIIHPIFG
                     YIFVLCVVFHVFLNWAWVKSNFIITKK"
     gene            22895..23932
                     /locus_tag="PLJ75_04510"
     CDS             22895..23932
                     /locus_tag="PLJ75_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPD04509.1"
                     /translation="MNYLLLAILGTMVILIILVLFLLLRNQPSDVASQINAAIANQLL
                     TFQSAIHNELNSTRQELSQSKDTIAQHTLKTIESINKIGQTIHTITQQQEEAQKLGQS
                     LKDLLQSPKLRGNYGEAILEEMLDRVLPKGIWEMQYAIEGREKVDAVVKMKDMIIPID
                     AKFPRDNYQKYLQAQSAEEKRNYWDKYVNDVKTQIKSISAKYVKPEKGTSEFALMFIP
                     SEAIYYETIAEKNYLGVPSDIYEFAQDQKVIPVSPNTFYAFLQLIVVGSRNLEIIKSV
                     KKIQGGLAELEKSFELFYNKYEDIGKNIEKAQEAYRIGDSHIKRYKNRLDSTLSLEEF
                     AVNDTALPDKK"
     assembly_gap    23962..24061
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24062..24339
                     /locus_tag="PLJ75_04515"
     CDS             <24062..24339
                     /locus_tag="PLJ75_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04510.1"
                     /translation="FLSIIENDEQNLTVCAQRKEDITRVFPEADVIEMPENDYGCRTI
                     VTRRDVAMTIAKRILSIDYYNFKDSVKENDRKHIYADIWGNSLQLRK"
     gene            24515..27856
                     /gene="recC"
                     /locus_tag="PLJ75_04520"
     CDS             24515..27856
                     /gene="recC"
                     /locus_tag="PLJ75_04520"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HPD04511.1"
                     /translation="MNLYTSNSLNFLAERLGHLLFEKKKDPLVQETIIVQSIGMQRWI
                     SLKLAQQFGVLLNCNFPFPQKFAEDVFSAVIPEYQLSSLYEPQSLVWTIMRLLQHCIN
                     EKPFQHIKQYLTVSDAISYIRLFQLSQRLAYMYDEYIVYYCNDIVQWENKNNADEWQI
                     MLWKYIYHAISIDPHDCHKAHLLNYFLEALKNNNTKKAMKAALPERIFLFGITALPEY
                     YIMLFKALSSFIDVYIFQLNPSKEYWFDIRSEQSITRIKKKLLAKKQMVDDFHFETGN
                     PLLASSGTLGREYIGLLLDYDAAENINDETQYQIGNNDTLLGHIQSDICCLINRGKDS
                     NAAKIPIKSNDDSITIHSCHSPLREVEVLRDYIYNILNNENIVPNDIVVMAPDIDKYS
                     PFIAAVFDEVMLKKEGLPLLPYTIADQSFKTASRYISAFVDLLDALTGRMEADIVMEL
                     LDYDEIATVFAISKQDVAVYQDWVSRVNVRWGKDAKHREQFIQKESKAFTWQYAMERF
                     LMGYAVPENTGIIDDIVPFDCVEGTAAQRLGNFIMFLKTLFSFHTIAGQKHSISEWAA
                     RLEQLMSSLLVNNDTVSNDIVFVQSLLQQLVATSATLQITDEIDFIVIKDWINTYLAG
                     QRVSTNFLRKGITFCQLLPMRSIPFQVVCLLGMNDGQFPRIDERLSFDILKNKEKQPR
                     CVRSKRNDDRYLFLESMLSAQKKLFISYEGQSPVDLSLKQPSLVVAELMDYIEQGFQC
                     SDTAQSISNFILTKHHLHRFHNNYFNNDSKKYSYSKLSYHESKAWYGSKHRYHLLVQT
                     PVAVDQMPFHITMDDFISFFKNPARYFIKNVLGISLAENVNDIASDEPFSINGLEHYQ
                     LTNELCQRLINGGDSYGYKVLLKKQGKLPDGDLGDIIFEDAQSQVMDFVAKVKSKITC
                     DAEQHELHYTSNNNLVITGNPYLYANQQIYYRYATIKPDDRLHALLWHLFVCATQSDE
                     CNDKFTTLLIGKDNDKNKYKELTYQHLLKEKSINDLEQLVSIFLQGQSTILPFFPKTS
                     YVYYNAVTDGTSSKSKNALYKARDAFYGDNGYESIDYDNPYIKRAFYKVDIFDDETLS
                     NDFDHLSQTIFGIVKDYESEEKELQ"
     gene            27853..31515
                     /gene="recB"
                     /locus_tag="PLJ75_04525"
     CDS             27853..31515
                     /gene="recB"
                     /locus_tag="PLJ75_04525"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HPD04512.1"
                     /translation="MNTIALPHKFTDYALDGINCIEASAGTGKTYTITHLFLRLIIEN
                     DLPLDAILVVTFTNAATDELRERIRRLLKTAYDVVDSNFDDAVINGYKKNDELISYLK
                     KLCKEHDSEDYRLQIALRLQLALHDIDNAHVYTIHSFCRQALSEFAFESGSLFGSEVV
                     TDASPYYRRVIEEFFYKEIAPLGKEFMYYFISKVPKKGDFVQWCMKIINKLPDDPDVT
                     ILPQIELNVEELKKKIIQLYQIIETIKETIKENSDDIKSNYDIACKNKVLNGISYKSA
                     HFDNLYNVLKKLNIYVLIEDKTEQLIRKFSLKSLMKSLNKEYTDTTKTYTFHTIFEQI
                     QQCRDIFTDIKLQVIAAYRHFLLHYDTYYNRYRDEWNILSFKDILKDMHTALKQGTDS
                     PLATMIRSHYKAALIDEFQDTDPLQYAIFTTIFNKDGYTLYIIGDPKQAIYSFRGADV
                     FAYCKARECAQTKYTLRHSFRSTQTLTKAINTIFGTMENPFVLDQIDYSGVESSMPPT
                     AFDNEPSLHIWFVDTEAGEKHVTIPKAKTIIAQRIAKEINTLVQEKPIQYSDIAVLVR
                     KHKEARGVQDILQRYGIHSVLYSTESIFESDEAMELLQVMEAIVNPSSAQKIFRALIT
                     SLYGYTAQHIVALNDDSKLDSITMRFMDYYNAWKSKGFIQCFQLMMHDDTINRHFNED
                     IDTVSSRLLHYPDGERVITNLYHCAEILSQAETTKKLDALSLVEWFKKSILIKPDVEE
                     NKLRLESDANAVKIVTVHFSKGLEYPVVFCPYLFNVGMLDDIIVFHGGDSNCTTNVCL
                     KLNADKDKTNGDNLYGIDYKHYVNQALKEQLAEDIRVAYVAMTRAKYRCYVGWGNINK
                     TNRSALSYLFHYRFANNTTIDNFLDNNFNNNAASNELKDGLKKLVNLSDGSIALEVFN
                     TVPLDKAMVTQVMDTIVVEPERIQKDISPGWQLTSYTALSYTKEDEKGDEVYASVPHD
                     EQHEPTIFTFPAGSVTGLCIHSIFEEVDFASGEDAMRTIVAEKLQHYRFDVSWTDTIV
                     TMVRNVLSVELNTAVPLNRVTRENRQHEIEFYYPVDRLNRDECVQLFSMNNLFDTHYG
                     SALQRLSFDHIQGFMHGYIDMVFRYNDAYYIVDWKTNQLGYTMDDYHRSKLKSAMIDH
                     FYFLQYHIYAVALHRHLAMLLGKSYNYNKHFGGVFYIFVRGVNAGKGGDYGIFYDKPD
                     CQFIEKLNNYLHAVKV"
     gene            31512..>32342
                     /locus_tag="PLJ75_04530"
     CDS             31512..>32342
                     /locus_tag="PLJ75_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD04513.1"
                     /translation="MMQQQSYTDSIHYYFADFICNKYNCNSEVVKALLFCVSYMTSYG
                     HSCLSLATIAHKKLDEIVFEDYTSIPELTFPDVTSLHTVLMECHAVKADDDDAIKPIV
                     YDNGNCYLYRYFNYEEIIAQWFLNSISKSHEIDMQQVNAVLETFEQKGMHFASLQKIA
                     QLAALHHTFCVISGGPGTGKTTTVAGIVYQLLTAHNTLSIALCAPTGKAASRLKESMK
                     QFKDKYCDDAIKDIFPVESSTIHRLLGYKKGALEFYYNEDNRLPYDVVIVDESSMVDI
                     A"
BASE COUNT        10315 a         5213 c         6827 g         9787 t
ORIGIN      
        1 caaagtatgg tgaagaacgc tgggcacgac gtatagctaa aaggattatc tataagcgca
       61 gtgagcagcc aattgaaaca accggacagc ttgcggatat tgttgtacag gctattccaa
      121 aacaatacag ggtaaaaaac attcatcccg ctacacgcgt ttttcaggct ttacgaatat
      181 atgtaaacga tgagttatca gctattggtg agtcattaaa agacgccata acatttttaa
      241 aaccaggggg catgattatt gcactatcat tccattcatt ggaagacagg attgttaaag
      301 atagatttag acgatgggca aagggttgta gttgtgataa cgatgggttg cactgtcaat
      361 gcaatagtgg tccgcttgtt gctgtgttga caaaaaaacc gttgcaaccg gaagaatctg
      421 aattagaaaa aaatcagcgt tcacgaagcg caaaattacg tgtatgcagg aagctttaat
      481 tattatgaac acatccgaac aaaaaagtat agtgcaatat agcaccattg ttatatgtgt
      541 catatgtttt attgtggcat ttatttatca aaatattgaa tatatgcagt tgaagatgca
      601 gtatgatgca atggttaatg aatataaaac tgttgttgag gagtatgatc gtcaaaagta
      661 tatattacaa aaagctgtgt caatgaaaaa tcttgatgcc tttgcacagt tgcatggatt
      721 ggtaccaatg agtaaggaca gtgtagtgat agttgaaaat aatagtaaaa acactatgca
      781 ggaacaaaga tgatggagat aaagatatac aattcatttg atttatatac attgctggat
      841 tcgatggatg ttagcatcat caaaggtgat gaagatattc gtatcagttc agttgaatat
      901 gattcgcgca gggttgaaaa agaatcgata tttgttgcga tagaagggtt tgaaactgac
      961 ggacataagt ttattaaaga cgcgctggaa aacaaagcat catgcattgt gcttaatgaa
     1021 aacagactgg atgaattttc ctttttactt gatcgtaatg atctggcagt agttaccgca
     1081 aaagatactc gtaaggctct ttcgcagata tctgcgcgtt tttatgggga gccttctctg
     1141 caggcagtgg taataggtgt taccggtacc aatggtaaaa caactacaac atacatgctg
     1201 gagtctattt tatcgaaagc aggatttcat tgcggtgtga taggcactat taactatcgc
     1261 tggaaggata aaaaaatcaa tgcattacac accacgcccg aatccaaaga ccttcacgag
     1321 atgatgtcaa taatggtgct ggatggagtg gattgtatta tcatggaggt ttcatctcat
     1381 gcgctttcat tgttgcgcgt tgatgatatt cattttgatt gtgctctatt taccaattta
     1441 acccgtgacc atcttgatta tcatataaca tttaatgact atttcatggc aaaggttcga
     1501 ttatttgaaa ttttaaataa aagttgtaaa caaaaaaaag cagcgtttat caatagcgat
     1561 gacaattacg ggagacagat cctgaactgg cggcagcgct ttaactatcc tcttttttcg
     1621 tatgggattc agaacaacgc tgactatcat tgtattgagt cgtccatcca gacatccatc
     1681 aggggtacgc ggtttgccac cgatgtgcct tttgcaaaac aatttgagat gcgtcttgcc
     1741 gggaaattca atgtttacaa tgctcttgct gctattggtg ttgcacattt tttaaatata
     1801 cctgcgggca ggatagtaac cggtattggt ctggttgcaa gtgttcccgg cagatttgat
     1861 atagtattat caccagaagg ttttgcagtt atagtggatt atgcacatac caacgatgcg
     1921 ctggaaaaat tgttactggg cgttcgctca ttaaatccac agcgtgttat tactgttttt
     1981 ggttgtggtg gcaacaggga caaaacaaag cgacctatta tgggtaaggt aagcgaggag
     2041 cattcggact ggattatagt gaccagtgat aatccaagaa aagaaaatcc tgaggatatt
     2101 atcaaggata taactgctgg catgaaatca aaaaagtatc agattattgt caaccgtgaa
     2161 gaggcaatta aacaggcaat agctatggct gaaagtggtg atgtgattgt tattgcaggc
     2221 aaggggcatg aagattacca gatcattggg acaaaaaaaa tacactttga tgataaagag
     2281 gtagcgttaa aatatatcaa tgaaaggaat gcaaagtgat agtgcagttt gaaacatctt
     2341 cagtaactat cgcccgagct ataggtgcaa agcattatgg tgctgatatg gctgttaagc
     2401 atatcacaac agattcacgg cttttagacg ataaaagttt gtttgttccg atagctggtc
     2461 agaaatttga cgggcatgat tttattgaag aggcgtgtaa aagcgggaaa tgctcttcgg
     2521 ttttgacaca caaacacact gatgttgcga ttgccgagag gtatggtgtc agcgctattg
     2581 tttgtgatga taccctgcac gcatatatgg atattgctct atggcaccgc aaaaattttg
     2641 atattccaac tattggtatt acgggaacca acgggaagac aaccacaaaa gagatgattg
     2701 ctaacgtttt agaatcgcag ggcagggtac tgaaaacaga aaaaaattat aacaacgaga
     2761 ttggtgtgcc gtatacactg ttgcatttaa acaaagagca tgcatttgca gtgatagaga
     2821 tgggaatgaa tcatttaggt gagatacacc gattatcact ggctgctgcg ccagatgttg
     2881 cagtaatcac caacattgga gaaggtcatc ttgaatttgt aggatcaacc atgaatgttg
     2941 cacatgcaaa agcagagatt ctggatggca tgaataaggg tatatgtatc atcaataatg
     3001 aaacaaagca taaggatatt gtcattaata aagctaacga caaaaagatt aaggttataa
     3061 catttggact taccggtgat atacgccctg caacctttgc tgtttatgcc gataaaaccg
     3121 tagtgcagta tgatggtatt gattttgttg tttatgcgta tggattccat aatgtgtatg
     3181 ctatgctggc aactattgca gttgcaaagg tattgggaat accgctgaac aatgttaaag
     3241 aatcccttga atgttttaag ccagtgtcga tgcgaagtgc tctacacaat ggccgttgta
     3301 taatcattga tgacagctac aatgcaaatc cgctttctat ggaatatgcg tttgccagtg
     3361 catcaatggt attcccccat aaacgtaaga ttgctgttct ttcagatatg aaagagcttg
     3421 gcaatgtttc aaaagaacta cactatgaaa ttggcgctaa attagcacac agcgggtttg
     3481 cttacctttt tatatggggc aacgatgcaa ggttttacgc tgatggcgcc attgcggcag
     3541 gttttgagcg ttcgcagatt tatatatatg atgacaaaga agagcttata caggctttga
     3601 gacagtttat tcaagctgat gatgtggttc ttgttaaagg ttcgcgttca atgaagatgg
     3661 aagaagtagt aaaagcatta gaataatgag gtatctatgt tttaccattt tattttacca
     3721 ttacatgaat atctttcatt tttacgattg ttccagtata ttaccttccg ttcggcgtat
     3781 gcagctttga catcgcttgt cattgtgttg ctgtttggtg gaatggttat acgctggcta
     3841 acctacctgc gctttaaaga agagatcaga gcattaggtc cccagtcaca tcaaataaaa
     3901 gcaggtacgc ccaccatggg tggcgtgctt gttttaggtt cggttcttat ctcggtgtta
     3961 ttatggggaa attttaataa tctgtatgta gtacttctgg tagttgttac ggttttggcg
     4021 ggagcattgg gctttgctga tgattatatt aaatctattt tgaagaaaaa ggatggtatt
     4081 ccggcacggt taaaattttt tatgcaatgt gttattgcct gtgcggttgt tgttgtcatc
     4141 tatctttttc cgtcaaacag taaagaggca acaacgctgt atgttccatt tattaacaaa
     4201 gcaattattg atttatcatg gtggtggatt atctttgcca ttctggttat tgttggcagc
     4261 tccaatgcgg taaacctgac cgatgggctt gatggccttg ccataggtac ggttaccata
     4321 gtggttggtg ttttagcagt aatgtcgtac gtgtcagggc atcagcctat tgcacagtat
     4381 ttgcgcatac cgtatattcc acaggcagca gagcttaccg tttttttagc agcgctgggt
     4441 ggtgctggac tgggtttttt gtggtttaat tccaatcccg caacagtatt tatgggggac
     4501 accggctcgc tggcacttgg cgggataata ggtactgttg ccattatgat taaaaaggag
     4561 atatttcttt ttattgttgg cggcattttt gttattgagg cactatcggt catcattcag
     4621 gttggttcgt acaaactaag gaaaaaacgc gtatttaaaa tggcgcccat tcatcatcac
     4681 tttgagctat caggcgtgcc agagcagaag gtggtagttc gtatgtggat agttggcatt
     4741 attttagcaa tactgggttt gagttcatta aagatattat aactatagga tagttatcgt
     4801 aatgaagaca cagcacatat tggttgttgg tttgggatat agaacagggc ttgcatcagc
     4861 caattttctt gttaacaagg gatttgatgt taccgtgtgt gatattaaat cttctgagga
     4921 actatcgccc ataatacaaa aacttgatgc acgggtaaag gtgattgccg gcaatcagca
     4981 tccatcattg ctggaaaatg gatttgatga gatagtattg agccccggag ttccacagtc
     5041 cattcccctg attcaggagg catatagaaa agctatcccc gtaatatccg agatagaatt
     5101 agcataccgc tattgtaaag ctgccatagt aggggttacc ggtaccgatg ggaaatcaac
     5161 ggtaaccatg ttgattgctc attgtcttac gcagctgggc tttgacgctc atccgggagg
     5221 taatattggc ataccgttta tttcgcttgt cgattcatta catgaagatt cagtggcagt
     5281 gattgagctg tcgtcatttc agcttgagac ggttaaaaca tttaaacctg atgtagcaat
     5341 cataaccaat cttactcctg atcatttaga tagatacaat tcattatatg attactttaa
     5401 cgctaagatg aatattgcca ttaatcagtg caaggatgat tattttattt ttaacaggga
     5461 tgatgaattt atagttaaca acatgcctgc actacaatcg caacaacatt cattttcctt
     5521 attggggaaa gcagattgtt tttacagcaa tggtgcaatt gttatggata gtaatggtga
     5581 aacaacgctt gttgcatatg ccagtaacat gaagatagca gggctgcata atgtgcagaa
     5641 tgctatggcg gctattctgg catcaacctt tatagggaaa aagtttggta acaatattga
     5701 tttaaaagtt ctggaagaag cgttatacag ttttaaagga ttgccgcaca gggtacaaaa
     5761 cattggcgaa tataagggaa ggatttttat taatgattca aaggcaacaa ccactggtgc
     5821 ggtaataatg gcattaaaaa gtttgcagga taatgtaata cttatccttg gtggcagggc
     5881 aaaaggcgat gattattcac gattgaaacc agtcataaaa ggtaaggtga aagcgttagt
     5941 gctcattggt gaaacaaaag atgaatttgc ctcaatcttc agcgatatac cgcatattaa
     6001 agcattttca atggaggatg ctttaataaa ggctatgaaa ctcagtgcga aaggtgatac
     6061 aatacttctt tctccagcct gcgcctcgtt tgatatgtat aaaaattatg aacaaagagg
     6121 gaatcacttt attgaaattt attctaaatt ggtgcgagga gaactggcgt ggatgtaaag
     6181 gcggtgatag acactcgcag aaaaggcgag ccagaaatag cactgtttat tgtgtcgttt
     6241 attcttattg gcatagggat tgcaatgagt tacagcgcca gtgcaatttt tgctgaacgg
     6301 gtgtttggtg attcgttata ttttttaaaa cggcagcttg tatggtgttg tctgggattt
     6361 tttataatga tgatagtaca gcatattgat tacagatatt accagcatta cacccgtata
     6421 atgctgttgt ttaccatgat tgcgcttatt ctggtattaa tacccggcgt aggtattacc
     6481 gttaaaggtt catctcgctg gctgggatat ggtcctttgc aattccagcc agcggaatta
     6541 gtgaaattat ttgttgttat atattttgca aaagtatttt catatgacaa tcatcgggaa
     6601 attaatgttt tacagctttt gttgccattg ctggtagtgg gtattttctt tttgcttatt
     6661 atgttgcagc ctgattttgg aacagctatt aatctggcca tcatttcggt agtgttactt
     6721 tttgtttccg gttttccatt gatatatatg ataggtctgg cattattgag tgtgccaatg
     6781 ttttatctgt taatatatca ggttgagtat agacgtcagc gcattttagc tttttttgat
     6841 ccatggtcgg ataggtttgg taagggatat catattatcc aatcatttat tgcatttaaa
     6901 ttaggaggta tgttaggtgc gggattgggt tcgggaactc agaagattaa gcgattaccg
     6961 gaaccacata ccgactttat atatgcagtc attgccgaag aagcaggttt attgggaaca
     7021 ttcagcattc ttattcttta tttatttttt ttctattttg gggttaaaat tgcactgaaa
     7081 gcaccggatg aatatgggag actactagca atggggttaa cacttcttat aacagttcag
     7141 gcatttctca atatcagtgt tgtcattggg ctgcttccaa caactggcat accgcttccg
     7201 tttataagtt atggcggttc ttcgttattg gttaatatga tggcaacagg aatattgttg
     7261 aacatttcaa aatatcgcga ggttgttcca agggatatta aatttgataa agaggtgctg
     7321 gtatgaaaac accagtattg ataagtggtg gcgggacagg cgggcatata atgccgggga
     7381 tagcgttgta tgaagagttg aaaagcaaag gatatgaggt attcctgctg gttggtaccg
     7441 gtgatgtgaa attttcatcc ctgaatagtg tggaaaatat ccaggtgtat aatgcgccaa
     7501 caatgtggaa aaatattgtt aattttccag tgtttacaat acgttttatt tcggcaatgc
     7561 tatggacaat gaagtttata gcaaaaaata atatagcagc agttattggt atgggaggat
     7621 atgtttcagc accatcgctt gttgcagcaa tgataaagaa gatacctata tatctatgtg
     7681 aacaaaatgt agtgcccggt atggtaacca atttttttgc aaagcgtgca agagcaatat
     7741 ttactacatt tgaagccact acaacatatt ttgatgtgga atcaactggt aaaattgtac
     7801 atgctggcaa ccccatacgg aaacaggtgt taacacctgt agaaaaagat gcagccttaa
     7861 aacgttttaa tatgatccac tgtaaaaagg taatattggt gatagggggc agtcagggtg
     7921 cattgaagct taatgaactt acatatgata tgcttgttag ctatccggat agatttgcag
     7981 atgtaggtat tatatggagt accggtacat attcatacca gcagtttaaa gaaaagctgc
     8041 ataccataaa gcatgcatca atttatcttg caccatatat tactgatgtt gaaaattcat
     8101 atgcagcatg tgatattgct attagccgtg ccggagcagg tgtcatgatg gagcttgcag
     8161 catggggtat tccttcaatt ttgattccat atccttttgc agcaaaggat catcagaaaa
     8221 aaaatgcaca ggagtttgtg aaagcaggtg ctgcagaaat gatcaataac agtgacgcaa
     8281 ctgcacaagt agttgccacg ctattgtttg atttacttga taatgaaacg ttattacgga
     8341 aaatgagtgc taaagcaaag tccattgcaa agcataatgc ggcagaaatt attattaatt
     8401 atattattaa agattctgat ttgaaaaaat aggagcgagt agtagtgttt agaaaatcca
     8461 aaaaaatgca ttttattggc attggtggca tcgggatgtc aggtattgcc gagattttaa
     8521 tcaatttggg ttatgatgtg tcaggttcag atttgaggga atcagagcaa accaaacggt
     8581 tgcgccaatt gggtgcaacc atttacattg gccattatcc atcaaacata aaggattacc
     8641 atgtggttgt aacatcaagc gctattagta aaaataatcc tgaaataata gaggcaaaga
     8701 aaagaaaaat accggtaata cacaggtcag aaatgcttgc tgaattggtg agattaaaac
     8761 atggaatagg tgttgcaggt acacatggga aaacaaccac ctcatccatg ttagcctata
     8821 ttttatatca tggaggatta aatcctactg ccgttgttgg tggcaaggtt ttaaattttg
     8881 gcagcaatgc aagaataggt gaagggcaat attttgtttt tgaggcggat gaatcggacg
     8941 ggtcatttct aaagctttta cctactattg ggattgtaac caatatcgat gcagatcatc
     9001 tggatcatta taaatatttt gagggcatta aagaagcatt cattacgtac atgaataata
     9061 tcccgtttta tggatattca gtagtttgca tggatgatcc ggttgtagct gaggtattgc
     9121 ccaggattga gcgtccctat gttacctatg gatttaatcc cggagctaat tttaaagcta
     9181 ccaatattaa gttattaaat ggatcaacaa ggtattcctg ttattataat gacaaattat
     9241 taggggagat agttcttaat ttattaggga agcataatat tattaattca ctggctgttg
     9301 tggctgttgc tcttgaattg ggattaacgt ttggtgttat agctgaagct atagctcagt
     9361 ttcaaggtgt tggaagacgt ctggaaaaga ttggtgaaac aaatgggatt atcgttgtcg
     9421 atgattatgg ccatcaccca acggagatac gggctaccct ggatgcgtta aaacaattag
     9481 gaaaaaggtt aatagtggtg tttcagccgc atcgttatac acgaactcaa tatttatatg
     9541 atgaatttgg tcagtctttt acaatggctg atgagctttt tttaactgaa atctatcctg
     9601 ctggtgaaga gcccattgaa ggagtaagca gcaatttaat acaacaatct gttaaaaagc
     9661 atgaaggcag ggatgttgtt gtgatacctc atcttgatca ggtgccggac tacataagtc
     9721 caatagtcag ggaaggtgat gtcatagtaa cattaggtgc aggcgatatc tataaggttg
     9781 ggcatgccat actttcaatg ttagataaaa agggtgtggt acagtgaaaa gtaacttata
     9841 cactgccagt atgattgtga gccttttact atttccctta tatggttatg catcggtaga
     9901 agcaattagc actgttaagc tttacggatt aaaatatgta acaaagtttg aaatattaaa
     9961 taatgccgtg attagcaaag atgataggtt ttttgttgat atgaagaagc tgaataattc
    10021 attacaaaac aatcctatga tagagtcgta tactgtggat acaaacaata acattttaac
    10081 tgttaaagta aaagaaaggg atatccttgc tattgtgctt attgaaaaag gcaaagatac
    10141 tataccaata gaggtcgata gtcatctaaa aataatttca acctatagag ttcatgcata
    10201 cgacaggccg ataataaaaa taacacagga tgaggtttat cataataaac tatcgccaag
    10261 aattataaaa attcttatga ttatgaataa acttaagaat gttcagggac aggctatagg
    10321 tagaatatcc gttattgatt gtattaatcc atttgtctcg tatgtttcaa ttcatggttt
    10381 acaaacacgt tatatatggg atgaaacata tgaagggatt tcactaatgt attcggttcc
    10441 tggattgctt gatagtaaat ctcattgtcc cggcaatggc gtggttacac aaaaaaggat
    10501 agtggtgtgg taaaactatg aaagaagata ccattatgga aaacattgta gtagggctgg
    10561 atataggcac aacaaaaaca tgtgcagtca ttggcttttt aaatgagaac aaacagattg
    10621 aagttgttgg tgttggggtg gctccttcaa aaggattaaa aagcggggtg atagttaata
    10681 ttgataatac agttgcatca attattaaag caatagatga tgcagaattg atgggaggtt
    10741 gtgaggttaa ttcggtattt gttgggataa ccgggcaaca tataaaaggg gaaaattcaa
    10801 gaggtgttgt tgcagttgct aaccgcaaca gaaccataac acctgttgag atgaaacggg
    10861 ttattgaggc agcacaggca attgttatcc ccatggacag ggaaattatc catgtattgt
    10921 ccaaagagtt tgctgttgat gatcaaacag gtattaaaga tcctataggt atgagcgggg
    10981 tacggctgga ggcagaggtc catattataa caggttctac aacaagcatt cagaatttag
    11041 ttaagtcagt taataaagca ggttttgtgt gcaatgatat agtgttttca ccgttagctt
    11101 cagccgagtc tacattgagt agagatgaaa aagatttagg agtagctctg gtagatattg
    11161 ggggtggcac gacggatatt atggttttta tagaaggtgg ggtggcatat tcagcagtat
    11221 tggggattgg aggcatccat gtaacaaacg atatatcaat aggtttacgt actcccattg
    11281 attctgccga ggttattaag aagaaatatg gatgtgcggt tgttgatctg gttgatgcat
    11341 ctgaaacaat tgaggttcca tcagttggcg ggagagcccc acgccgattg ttcagacatg
    11401 aactggcaca gattattgaa cccagagtaa tagaaataat ggagatgatt gataacgaat
    11461 tggtcaaaag cggcaaaaag gaaatattgg ctgcaggtgt tgtcctgacc ggcggaggca
    11521 gcatgattga aggaactgtg gatgcagccg aaagagtatt gaatatgcct gtcagggttg
    11581 gcttcccgga gaacatcgtt ggtcttaaag atgttgtatc aaccccgatg tatgcaaatg
    11641 gagttggatt gttgcgttat ggcgctaaaa tggggcaatt acggcaaacc agaaaaacgc
    11701 ctggaggatt aaaaagtctt aaggataaat tgcgtcaaat ttttaatgat tatctttaat
    11761 atgagcatta cagtaatttt tttataaatt tttttaaaga ataatgcgac ataatataaa
    11821 aggaggcaat aatgtttaaa tttgatgaag aaccacagat gaagactatt attaaagtag
    11881 ttggggttgg aggagccggt aacaatgcag tcaacagaat gatagctaca ggtttagaag
    11941 gagttgatta tattgttgta aatactgact cgcaacagct taaagcatca ttagctcaga
    12001 caaaaattca gattggaaca aagttaacac gaggtttggg agcaggtgcg gacccatcaa
    12061 taggcaggga agcagcattg gaagacaggg ataagctgga aaaagggctg aagggagctg
    12121 atatggtgtt catcactgcc gggatgggtg gtggaacggg aacaggtgct gcacctatag
    12181 ttgcagaggt ggctaaagaa agtggagcgc ttgttgttgg agtggttacc aaaccattca
    12241 gagctgaagg caggagaaga atgcaacatg ctgacgaagg tattaaaaat cttaaggaaa
    12301 aagttgatac catcattgta atacctaacg acctgttatt gaagataatt gatcgtagta
    12361 cgccaatgga tcatgcgttt aaattagcag atgatatttt acgccagggt gtgcagggta
    12421 ttgctgatat aatcatggta accggactgg tcaatgtaga ttttgctgat gtgcggacgg
    12481 taatgaaaga aacaggtgat gcaataatgg gtgttggtat tggagtagga gaaaatagag
    12541 caatagaggc aacacagatg gcaattaaca gtccattgct tgaagaatca ggtattgaag
    12601 gtgcaaaagc ggtattgctc aacattgctg gtggcagtga tatgtcatta catgaggtaa
    12661 acgagataac agaaattatt aacaaacagg ttgatcctga tgcaaacata atttttggag
    12721 caactatcga ccctgcctta gatgataaga tacgggtaac ggtgatagca actggttttg
    12781 accgtaaact caatatgctg aacagaccta atgagctgga tagagcaacg gggacgccat
    12841 tgacagtgat tgaagggaag aatcataagg agcggattga aaaaaaggtt cctgctcaga
    12901 tgaagtcatt tacgctggac tatgaaaaac gaagggttaa agattactcg catgaggatc
    12961 ttgatatacc ggcgttttta cgaagaaatg cagaataaaa gtaagaaact atcgcatatc
    13021 ttcgataatt tttaatgagc ttgatgtttg tattgaggat gatgactcaa aaagcaatat
    13081 gatctcagta cgtttatttt tctgaataac aaaataaaat tcagatgcta attttcgcac
    13141 aagatcaatg ccgcgtcctg tctcagaaat tttatcggtt atatcttcgc ctgttttagc
    13201 tgcatgtagt atgagctgtt cctgttcaat ggctttataa atgctttcta gtattttccc
    13261 ttttgatagc gtcccctggt aatccgtgac tgcaatacca tatgtggagg cattatgaca
    13321 aaatgaaata tcaacgtatt gcccttcttt cagttcaata ggaactcgca tttctttttc
    13381 atgtgtatat ccatgtgaat ggtaaacggc atttataatc atttcattta gtattaaact
    13441 tagaactact ttatttttaa tggtaaatcc ccatgattca atggttgcaa gtatagtatc
    13501 aactcccgat tgaatttgtt ttgaagcgtt aatgcggatg gaattagttt caattatatt
    13561 atgaatataa tttgacaatc caaatacatt gttccgtgat attattttat cgaccagttg
    13621 cacaaattca gttttattca ccggaatttt taaaacattt cctattccct gtctgaatat
    13681 ttcatcaaaa aaaatatcta tattgctttc ggttatcatt atagcaggta tataggtata
    13741 atgattttgg agatagttat aaagatcaaa agcattatcg ccaggcaaag caacctgtga
    13801 aataaccaga tcaatgtcgt gcatatttaa tagtgaaata gcgtcaaatg cacaatcagc
    13861 aaaaagcacc gaatagtatg acgaaaaata atcttttagt atgtttttgt aatcggtatg
    13921 atctactatt aatattgtgt tcatgatgat aacattcccc taatggtgca ttgaataata
    13981 tgtaacaaac tatgcataat atttcaatta ttattttaac gtatgatcat ctctaaaaat
    14041 gttttttaac tataaacaat atgatgcaac atcattataa tacttgtacc catgaagaac
    14101 tttcaaaaat ctaattatga tggtttcccg tatgattcac aaagttttta atattatatt
    14161 ctatttaaat caagaaatgt aatttttttt aacaagagct aaaaagcata ttatcatagc
    14221 attgaagcaa aacatgggaa tatcatgtaa agcactttat aatgaagata caatgggcta
    14281 aataatgact gtaaccatgt ataattttga aaaaatgaat ttatggtgaa taattttaat
    14341 aaaatcattg acaagctttc taaatataag ctataattgt ttcaaagtgt tattcattgg
    14401 attatttggt caaaatttgt gaggtgaaag atgaaaaaaa taatgatgtt aacattatgt
    14461 attgttttct cactggcaat aatttcagat agcagtattg ctcaggatac cgtaaaaaag
    14521 ggtacaaaag aatatattgc tgatctttca agcaatgatg aagcagtagt agtggctgca
    14581 gaggagtgga tagctgataa taaggaaaaa tctgcattat caaggttgca ggaattattg
    14641 cgaagtgata atcgagtaaa ggtgcgttta tatgctgcaa ttgccttggg cgagattgcc
    14701 gatgagtctg ctgcagaaag tttaaataat gctctactta atgatccaag tgctgatgta
    14761 cggtatactg cactgctggg gattgcacgg ataggttcaa aagattcata tgatgccata
    14821 cagaaagccc gtgaaagtga gcaggatcct tttatcaaag atttaattca aaaaatggaa
    14881 gataaattaa aaggtaaatg atatcgtagc gtctaaaaat ctatttagac ggtaatgctg
    14941 tacataagtt tgttggtata cagtaagcaa tagattaaca gcccacgggt tagaccgtgg
    15001 gctgattatt acatgaatta ttacatgaag atgtaatttt taattaattt tcaaaggaat
    15061 gttaccgtgg aacaacaggt aaaatatgta gtttttgata tagaatctat acctaatgca
    15121 cctttaataa aacaggtaaa atatccgcat ttatctattg gcgagcatga tgcggttata
    15181 aaatttcagg aagagattct caacgtttcg gatggttcaa catggtttat ccctgtgaca
    15241 tttcaactgc ctgttatagt ttcaatgctt gaggttgatg aaaaattttt ccccatacgg
    15301 atgcatacat tggatgaacc tcaattcagg actgaagtta ttgtcaatca gttttgggag
    15361 gcaatagaaa gcatatataa tgatgcttca tacgttactt ttaatggtag aggttttgat
    15421 tttcctttga tggagttgat ggctttcagg tttggcgtta cggcaaagcg tcattttaag
    15481 gataaatttg cttcaaggtt tagatttggt acaaaacata ttgatattca tgattggctg
    15541 tcaaactaca gtgcaatcag gatgcaggga ggattgaatt tactggcaaa agttattgat
    15601 aagcctggca aggttgaaat gaccggcgat gatgtttatg atctcttcaa agaagggaag
    15661 ttacaatcca ttcacgaata ttgtatatct gatgtcatgg acacttattt tgtgttttta
    15721 cgaacacgag ttatgattgg agaattaagt cgtgagcggg aaggtgttat tattcagcat
    15781 acacgcgatt ttctggaaaa gaataaatct gctgtcaaag gatttgagca atatttaaca
    15841 aactggaaat gaattttttg caatggcagg aatgtacatg gtaaaaaacc tgccattgct
    15901 atgagcagta attgtttaac tattttctaa ctttcaaaaa cagcgcctct atctgcggat
    15961 gaaaccataa gactgtatcg gtacatatag ccactggtaa tctttggttt tggagctttc
    16021 cattttgcaa ggcgagcctt tatttccttg tcatctaatt taagggttaa ttttttagcc
    16081 ggtatatcaa tttcaataat atcaccatct ttgacaattg caatggggcc tccggaggct
    16141 gcttctggtg atatatgacc tatagaagaa cctcgtgttg caccggaaaa cctgccatcg
    16201 gttataagtg caacttcttt atccatgccc ataccagcta taacggacgt gggagtgagc
    16261 atctcacgca taccgggacc accgcgcggt ccttcataac gtataacgat tacatctcct
    16321 ttttgtatct gcccgcccat aattgcttta accgcttctt cttctgaatc aaaaaccttt
    16381 gcaggtccac tgtgtttata catggatgga tcaacagcag cctgtttgac aatacaacca
    16441 tcaggagcta agtttccggt aagcaccgcc aatcctccat ccttatgcac tggtttagtt
    16501 acatttcgta taaccgatgt atcaaatatg gttttgtctt taacattttg tgctattgac
    16561 ttaccggtta ccgtcatggc attgccatgg attagcccat gctttaatag ttcatttatt
    16621 aaagcaggca tcccaccagc atagtgcaag tcaaccatgt gatgaggacc cgctggagca
    16681 atgctgcaaa gatgaggtat gcgttcggat atctgctgta tatctttaag ccgtatgggg
    16741 attcccgcat agtgcgatat tgcagggata tgaagtgcag tgttagaaga tccacctatt
    16801 gccatatcaa cagaaagcgc attctcgatt gctttttttg ttactatttt ttttggtgtt
    16861 aattgctttt tgacaagttc aactattttt ttaccgcttt cctttgctaa acggatacga
    16921 tcggccatta ctgctggaat agtaccattg taaggaagtg caagccctaa tgcttcagta
    16981 agacagttca tggaatttgc agtataaagc cctgcacatg aaccagcgcc tgagcaggct
    17041 gagcattcta tttcataaag atcttctttg gaaataagcc cttttgcagc tttacctaca
    17101 gcttcaaaga cctcgtcaag tccaatagct ttccccttat aattgcccgg aagcattgga
    17161 ccaccagaaa ctatgattga tggtatatca acctttacag cagcaattat catgccggga
    17221 actatcttgt cacaattagg taccatgaca agaccatcaa atgcatgcgc ttttgccata
    17281 atttcaattg aatcagctat aagctcacgt gaccctaatg aatatttcat gcctatatgg
    17341 ttcatcgcaa taccatcgca tacgccaatt gttggaaatt caattggcgt accaccagcg
    17401 gcgataacac cttttttaac cgcttcaacg atatttttaa gatgaatatg accgggaatt
    17461 atttcattaa atgagttaac aacgccaatt aatggttttg ataactcgtc atcagttaat
    17521 cctaacgctt taaaaagtga acgatgaggt gctttttcaa tacctttctt aacacggtcg
    17581 cttatcataa tacaaaattc ctcctttata tatagacnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnntat atatagacaa taattaatgt atacataata gtatatatat
    17761 atattgtaaa ttaaaattta ctaaaaaaag acatcattta aaaattactt tataggcgga
    17821 ttgcgcgcca ggtggaataa aatctatagg ttcaaaagta atattggaaa tattgtttgc
    17881 attttctttt actgaattgg ttactaaaac ttcccatgct tctgcaatat cctcacctaa
    17941 tttactggca gcattaacct ctgcaccgta tacatcttca tcgccaatgc gaagcatggt
    18001 gccaaatcct aaacctacgc ataacacaat gtggtcttct tcatgtttat tgttattata
    18061 atcgtataaa acctgatgca ttgttttgca acattccaaa cctttaacaa tatttctgaa
    18121 aataacaagc atgctatcgc cttcagtctt taataaaatt ccgtcatatt cttcaatgat
    18181 tggtacaaga aggcgttgcg attcatagat ggtctgtaaa aaatgaatga tgccataggt
    18241 tgccaccttt cgggaaaaag cggctaaatc agtaaacata acgcaccata cttcaccaaa
    18301 caaatcccat atccttttat caattaattc tttattgaca tccggcttca aacgtttttg
    18361 aattaatttt tcaagacgat cttcggatgc tccaaccgaa attactcttc gtaatgccat
    18421 gagttcatcc tccaaaaata gtattattac ttatcgtcat tgcgggcttg acccgaaatc
    18481 tactttacta ccaccgtcat tgggggcttg atctggaatc caattaatta ttgtgagatc
    18541 ctgaaataat cctgaatcag agttcaggat gacttattac gtttcgggat gaaacttcgc
    18601 acgctgtcat tctaagaaag cgttagcaat tgaagaatct attctttcat aaaacgatga
    18661 gattcttcac tctattcaga atgacatgtc accacattgt ctatggcata acacaagatc
    18721 acgataataa aaaaggaatg aaaatagtga aatataaaaa atgtataatg tcaagaacta
    18781 tacttgggga gcactaatta ttataaaaac ctcttgacaa aaagctatta caccctttac
    18841 aactatacat cacattagat gtatatcata taattatact atgtttaacc cactaaaaat
    18901 atttaaaaat aaaaaagttg gtctggcact tggcagtggt ggtgctaaag ggatagcgca
    18961 tgttgctgtt attgattatt gcgaaaagaa tgcaatcccc attaatataa tcgccggttc
    19021 cagcattggt gcggttattg ctgcactgta tgcgtatggc agccttaagg agtttgtgtc
    19081 atttttaagg cttgttaaaa gcaatgatat attttcatat tttgatcctg tatttccaaa
    19141 aacaggattg atacagggca ggcggatgat tgaactttta gcaaaattta taccaaaaag
    19201 tgtttcaatt gaagatttaa agattccggt agcgatagtt gctactgatt tttacagtgg
    19261 caatcctgtt gtttttaaaa aaggaaatgt ccttgaggca atcagggcaa gcatatccat
    19321 ccccgggatt tttgttccgg ttagatatat ggacaccgtg ctactggatg gcggtgttgc
    19381 caatcctctg ccaattgatg tggtaaaaca gatgggtgcc ggtatcacga tagcggttaa
    19441 tctacatcca accattaaag taaaaaaggc taagattgcg gcaatgaaca gtttacagga
    19501 actatcgccc aaagaagaaa attcttcaaa tgatattatt gaacatgttg ataaggagcc
    19561 agtcgatact ttatcgaaga ttacacagcg atcaggcggt tggataaata aagcaaaaca
    19621 gtggttggga atgaacagtg agggcaatgc agcaaaagac aacataccaa atatttttga
    19681 gatagtttcc ggtaccattg acatcatgga gtttatgaat acaacactta tgataaccta
    19741 taataagcct gatgtgttga ttgagcctga tcttttgcag gtttcaacgc ttgattttac
    19801 aaaagttgat atgcttttgc aggaagggca tgcagcatgt gaacgggtgc aaaaagatat
    19861 taaacataaa gttttgcgat gggtatagac tatggcatta acaaaagccc aacagttaga
    19921 agatttgcgt gttcacggta ttggcaactg caagcggtgt aagctgcatg caacgcgtag
    19981 caccatagtc tttggcgaag gtaatcctga agcacgtgtc atgtttattg gcgagggtcc
    20041 cggcaaagac gaagatgcgc agggcaggcc ctttgttggt aaagcaggac agcttttaac
    20101 agcggtgatt gaaaaaggga tgaagcttaa acgcagtgat gtgtatatag ccaatgttgt
    20161 taagtgcaga ccaacggtta atatggagtt aaagcgtgac cgtccacctg atgatgaaga
    20221 aacagctgcc tgtggtggat ttttaaaaca gcagattgac atcatacaac cagaagttat
    20281 agtaacgctg ggcaacccat ctacaaaatt tttactcaat tcaaaagaag gcataacaaa
    20341 aatcagaggt gtgtggcagc tttataacgg ttttccggtt atgccaacat ttcacccatc
    20401 gtatattttg cgcaacggtg gtgatgccag cccactgaag cgtctgttgt gggaggacat
    20461 aaagaatgtc cttgcttatt tacatggtga agtttcttct gccgatattg ctaaaaaaat
    20521 aaaaacatta cccgagttaa gtttacatga tacaactgag aataaacgtg atgatgaggt
    20581 acagggaaat ttgttttaaa ttattttgtt cgatagttac tgataatgga ttaacagtaa
    20641 ctatcgaaca tagagtatgt aagtagctgg ttgctttaag cactgttgct tttaataaaa
    20701 attttcttgt tatggagtaa gcataaatgt attatttatt ataaggaaaa agctgtagct
    20761 tttgattaaa tgatatacaa tatataacaa cttatttaaa aactttttgt gggggtattt
    20821 atgtcaacaa tcgaggtgaa agaagttaaa gacagtaaaa tgatgaaaca gtttattatg
    20881 tttccctgga cttcaaagat ttatgaaaat gatccggcgt gggttcctcc catcatcggt
    20941 gatatgaaaa aactttttaa tcccaaaaaa ggttattttt acgatattgg cgaggttgcc
    21001 tattttttag cgtataaaga tggtgtgcct gccggaagga ttacagctca tgttaaccgg
    21061 atgtacgagg aaaaatataa taaagaaaca ggcttttttg gtttttatga ggcaattgat
    21121 gatctggatg ttgcacgtgc attgtttgct gttgcggaag gttggttaaa gaaaaaaggc
    21181 aaaacgcgcg tagaaggtcc gcaaagcttt tctatttatg attcggttgg atttgaggtg
    21241 tttggtgagg agattatgcc agtggttggg ctacttcact ttgcaccgta ctataaagat
    21301 catgccaccg cttgcggtta caccaagtgc attgactggc attgcttttt agtaaaaaag
    21361 atagatgatt tcaaaccgta tttaaaagat gtccgcgaaa acatcatgaa aaatcaggat
    21421 gttaaatatg tatattatac tccaaaggaa ttaaagaagc gcgcaaaaga aatccatgaa
    21481 atttttaacc gagcatggga cggcaactgg gggcatctgc ctttaactga caagcaattt
    21541 aaaatgcttg ttgatgagct taaacttgtt gcagtaccag agcttattgt atttgctgaa
    21601 aaagatggca ggaccattgg ttttattgtt tcgctacctg acatgaaccc tgcattaaaa
    21661 atattgaacg gcaggcttta tccctggcga ttgataaagg cattgcggtc tataaaaaag
    21721 gttaaaagga tacgaactat catcatggga gtattgcctg aatatcgagg acagaagatt
    21781 gatgatgtgt tctatttgtt tacaatagaa aatggaatac ggttgggtta tgttgagtct
    21841 gattgctcgc ttattgttga aacaaatcaa aagatgatag gagcgctgaa accgctaaaa
    21901 gggcaaatat ataaaaccta tcgcatatac gaaaagcaca tatgattaaa aacacaaagc
    21961 gcttgcaaca gtttgaaaaa gagtttgttc agaagaactc cttaagccac cttcaggcat
    22021 taaaattgct cgatggtatg tgggagatgg gaatgacttt aggagttctt ccgccagacg
    22081 atccatggga agggattgaa gttgatatta gagttgcaag gatagtaaat tgttttaaag
    22141 aagaaataag ttttggtatg tatgctacct tgaaatcatg gtaaggcata tagtttttta
    22201 tgcaaggact aatgaaaaag gcctataaga tttcaaaaaa agagatattg agagccgtgg
    22261 aataaaaaaa attatagtaa gatattataa tcacatttga tgattagcag agtggtagat
    22321 tatataaaaa ataaaaaaaa gggaagaagt tttggaatgg acatacatca aataaaaagg
    22381 agaattttta tgaataaaca aaaattgctt aagccagtca atgtaatgat ggctatagac
    22441 tttgtttgtc tatctggaac tgccatgctt gatgatgcta ttccaaaaga tatttatgga
    22501 attattcatc caatatttgg atatatattt gtgctatgtg tggtattcca cgttttttta
    22561 aattgggcat gggttaagag caattttatt ataacaaaaa agtagaatta tttattttgt
    22621 cgtattatta agttgctatc ttacgcctta atttaaaaag ctggcatcaa aagccaacac
    22681 aactgccatg ctgaatcatg tccttgactg attttaagaa ttattttcag gatatcgtat
    22741 aatgattttg attcatgaat aaagcctgta atgacatagc gaaataatac tatttcatga
    22801 tgagtgaagc acttcttcag tgttttttct tgtagcgtaa attgtcaaat tttgtaaagc
    22861 tgaataataa ttaatttttg ggggctatat cattatgaat tatctattac tggctatcct
    22921 tggcacaatg gtaatattaa tcattctggt tttattttta ctgctgcgaa atcaaccatc
    22981 tgatgttgct tcgcagatta atgcagccat tgccaaccaa ttgttaacat ttcaatctgc
    23041 aatccataac gaattaaact ctaccaggca ggaactgtca caatccaaag atactatcgc
    23101 acagcatacc ttaaaaacta ttgaatcaat aaacaagatt gggcagacaa tccataccat
    23161 tacacagcag caggaagagg cacagaagtt aggacagtca ttgaaggatt tgttgcagtc
    23221 gccgaagttg cggggtaatt atggcgaagc aattttagag gagatgctgg atagggtgct
    23281 gccaaaagga atctgggaaa tgcaatatgc cattgagggg cgtgaaaagg ttgatgcagt
    23341 ggtgaagatg aaagacatga taatccccat tgatgcaaaa tttcctcgtg ataattatca
    23401 aaaatatttg caggctcagt cggcagaaga aaaacgaaat tactgggaca aatatgtcaa
    23461 cgatgtaaaa acacagatta aaagtatcag cgctaaatat gtaaagcccg agaaggggac
    23521 ttctgagttt gcattaatgt ttataccatc cgaggctatc tattatgaaa ctatcgccga
    23581 gaaaaattat ttaggagttc caagtgacat ctatgagttt gcgcaggatc aaaaagttat
    23641 accggtaagc cccaatacat tttatgcatt tttgcagctt atagtggttg gttcacgaaa
    23701 tctggaaatt ataaaaagtg taaaaaagat tcaaggcggg cttgctgagc ttgaaaaatc
    23761 atttgagctg ttttataata aatacgaaga tattggtaaa aatattgaaa aggctcagga
    23821 agcatatcgt attggcgata gtcacataaa acggtacaaa aatagattgg atagcaccct
    23881 gagtcttgag gagtttgcag taaacgacac tgctctgcca gataaaaaat aattgacatt
    23941 gaaaagtaaa ccggctatta tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 ngctttttat caataataga aaatgatgag caaaacttaa cggtttgtgc acaaaggaag
    24121 gaggatatta caagagtttt tcctgaagca gatgtcattg aaatgccgga aaacgattat
    24181 gggtgcagaa caatagtaac acgcagggat gtcgcaatga ctatcgccaa aaggatactg
    24241 agtatagatt attacaactt taaggacagt gttaaggaaa atgacagaaa gcatatctat
    24301 gcagatatat ggggtaactc cttgcagttg aggaaataat acactattat aagaagttat
    24361 gatagttaat agcaaaacac atttactgca aaaaagcttc atggatgtat ttattattga
    24421 catgggattt accaatggca tattttatgg aagaagcgtg tacattccat agtaactatc
    24481 gtacacaaat actatacagg tgattgagca tactatgaat ttatatacaa gcaacagcct
    24541 caattttctt gccgaaaggt tagggcattt gctttttgaa aaaaagaagg acccgcttgt
    24601 acaggaaact atcattgttc agagtattgg catgcagcga tggatttcgc tgaaacttgc
    24661 tcaacagttt ggtgtactct taaactgtaa ttttccgttt ccgcaaaaat ttgctgagga
    24721 tgtttttagc gcagtgattc ctgagtatca actgtcgtct ttgtatgagc ctcaatcact
    24781 ggtgtggaca atcatgcgat tgttgcagca ctgtatcaat gaaaaaccat tccagcatat
    24841 caaacaatat ttaactgtgt cagatgcaat aagttatata cgcttgtttc agctttcaca
    24901 aaggttagcg tatatgtacg atgaatacat tgtatattat tgtaatgata tagtgcagtg
    24961 ggaaaataaa aacaatgctg atgaatggca gatcatgttg tggaaatata tttaccatgc
    25021 aatttcaatt gacccccatg attgtcataa agcacacctg ttaaactatt ttttagaggc
    25081 tctgaaaaac aataacacta aaaaggctat gaaagctgcg ttgccggagc gtatattttt
    25141 atttggtata acagcattac ccgagtatta tataatgctt tttaaagctt tgagttcatt
    25201 cattgatgtg tatatatttc agctcaatcc ttcaaaggaa tactggtttg atatacgttc
    25261 cgagcaatcc attacccgta tcaaaaagaa gctgctggca aaaaaacaaa tggttgatga
    25321 ttttcatttt gaaacaggca atccgctgtt agcttcatct ggcacactgg gcagggagta
    25381 tattggtttg cttttagact atgacgctgc agaaaatatc aacgatgaaa cgcagtatca
    25441 gattgggaac aatgacacat tgcttggtca tatacaatca gatatatgtt gccttataaa
    25501 tcgtggtaag gattccaatg cagcgaagat accaataaaa tcaaatgatg attcaatcac
    25561 cattcattcc tgccattctc cactccgtga ggttgaagta ttacgcgatt atatctacaa
    25621 catattgaat aacgaaaata ttgtacccaa tgatattgtt gtgatggcac ctgatataga
    25681 taagtactcg ccatttattg cagcagtgtt tgatgaggtt atgctaaaaa aagaaggact
    25741 gccgttgttg ccatacacta ttgccgacca gtcatttaaa acagcaagcc gatacatctc
    25801 agcatttgtt gatttgcttg atgcgttaac gggaaggatg gaggcagata ttgttatgga
    25861 acttctggac tatgacgaga tagcaacggt gtttgctata agcaaacagg atgtcgcagt
    25921 atatcaggat tgggtttcgc gtgtaaatgt acggtggggg aaagatgcaa agcatagaga
    25981 acagtttata caaaaagaaa gcaaagcctt cacatggcaa tatgcaatgg agcgcttctt
    26041 gatgggatat gcagtcccgg aaaatacagg aattattgat gatattgtgc cgtttgattg
    26101 tgttgaggga acagcagctc aacgattggg taattttatc atgtttctga aaacactatt
    26161 ttcttttcac acaattgccg gtcaaaagca tagcataagc gaatgggcag cacgattaga
    26221 gcagctaatg tcttcgctgc ttgtcaataa cgataccgtt agcaatgata ttgtttttgt
    26281 tcaatcgttg ctgcagcagc ttgtagcaac ttctgcaaca ttacagataa ccgatgaaat
    26341 tgattttatt gttataaagg attggatcaa tacctattta gccgggcagc gcgttagcac
    26401 caatttttta cgtaaaggta ttaccttttg tcagcttctt cccatgcgaa gtatcccctt
    26461 tcaggtggtt tgtcttttag gaatgaatga tggtcagttc ccacgcattg atgaacgctt
    26521 aagttttgat atcctgaaaa acaaagaaaa gcagccgcgc tgtgttcgtt caaagcgcaa
    26581 tgatgatcgc tatctttttc tggagagcat gctatcggcg cagaagaaat tgtttataag
    26641 ctatgaaggt caaagccctg ttgatttaag cctgaaacaa ccttcactgg ttgtagctga
    26701 acttatggat tatattgagc agggttttca atgcagcgat accgctcaat caatcagcaa
    26761 tttcatacta acaaagcatc acctgcacag gtttcataat aactatttta acaatgacag
    26821 caaaaaatat tcatattcaa aactatcgta ccatgaaagt aaggcatggt atggtagcaa
    26881 gcataggtat cacctattgg tacaaactcc agtggctgtg gatcaaatgc cctttcatat
    26941 taccatggat gattttatat ctttttttaa aaatccagca cgctacttca taaaaaatgt
    27001 attgggaata tcgctggcag aaaatgtcaa tgatattgca agcgatgaac ctttttcaat
    27061 caatggactg gaacactatc agttgaccaa tgagctgtgt caaaggctca ttaatggtgg
    27121 cgatagttat ggctataagg ttttgttgaa gaaacagggg aaactccctg atggtgattt
    27181 aggggacatc atttttgaag atgcgcagtc acaggttatg gattttgttg caaaggtaaa
    27241 gagcaagatc acctgcgatg cagaacaaca tgagttgcac tatacaagca ataataacct
    27301 tgtgattaca gggaatcctt acctgtacgc aaaccagcag atatattacc gatatgccac
    27361 aataaaacca gatgacagat tgcatgcatt gttatggcat ttatttgtat gcgcaacaca
    27421 aagcgatgaa tgtaatgata aatttaccac actactcatt ggtaaggata atgataaaaa
    27481 taaatataaa gagttaacct atcaacatct attaaaagaa aaatcaatca atgatcttga
    27541 gcagcttgtt agtatatttt tgcaagggca atcaaccata ctgccttttt ttccaaaaac
    27601 atcgtatgta tattataatg cagtcaccga tggtacaagc tctaaaagca aaaatgcact
    27661 atataaggca cgtgatgcct tttatggtga taatggctat gaaagtattg actatgataa
    27721 tccttacatt aagagggcat tctataaggt tgatatcttt gatgatgaaa cgttgtctaa
    27781 tgactttgat catctatcac aaacaatttt tgggatagta aaagattatg aatctgaaga
    27841 aaaggaattg caatgaacac tatagctcta ccacataagt ttactgatta tgcacttgat
    27901 ggcattaact gcattgaagc aagtgctggt accggcaaaa catataccat aacccatctt
    27961 tttttgcgcc tcatcatcga aaatgacctt ccactggatg ctattcttgt tgtaaccttt
    28021 accaatgctg caaccgacga attgcgtgaa cggatacgcc gccttttaaa gacagcgtac
    28081 gatgtagttg atagtaattt tgatgatgcc gtaatcaatg gatataaaaa aaatgatgaa
    28141 ttaatcagct atctaaaaaa attatgtaag gagcatgatt cagaagacta tcgcctgcaa
    28201 atagcattac gattacaatt agcattgcat gatattgata atgcacatgt ttacaccatt
    28261 cacagctttt gcagacaggc attgagcgag tttgcatttg aaagcggttc cctctttggc
    28321 agtgaggtgg ttacggatgc ttcaccatac taccgtcgtg tcattgaaga atttttttat
    28381 aaagaaattg caccactggg caaggaattc atgtattatt ttataagcaa ggtccccaaa
    28441 aagggtgatt tcgttcaatg gtgcatgaag attatcaaca agctgcctga tgatccggat
    28501 gttactatcc taccacaaat agaattgaat gttgaagagc ttaaaaaaaa gattatacaa
    28561 ttatatcaaa ttattgaaac tataaaagaa acaataaaag aaaatagcga tgatattaaa
    28621 agcaattatg atattgcttg caaaaataaa gtattaaatg gcatatcata taaaagtgct
    28681 cattttgata atctgtataa tgttttaaaa aagctaaata tatatgtgtt gattgaagat
    28741 aaaacagaac aacttatacg taaattttct ttgaaatcat tgatgaaaag tttaaataaa
    28801 gagtatacag ataccacaaa aacatatact ttccacacca tatttgaaca aatacaacag
    28861 tgccgtgata tctttactga cattaagctg caggttattg ctgcatatcg tcattttctt
    28921 ttacactatg atacatacta taacagatac cgtgatgaat ggaatatact gtcgttcaag
    28981 gatatattaa aggatatgca cactgcacta aagcagggta ctgatagccc gcttgcaacg
    29041 atgatacgct cacactataa agcagctctt attgatgaat ttcaggatac tgatccgctg
    29101 caatatgcaa tatttaccac catcttcaat aaagatggct acacgctgta tatcattggt
    29161 gatccaaaac aggcaatata cagcttcaga ggtgctgatg tctttgccta ttgtaaagcg
    29221 cgtgaatgtg cacaaacaaa atatacatta cgccatagct tccgttccac tcaaacactg
    29281 acaaaggcta tcaatacaat ttttggcacc atggaaaatc cctttgtgct ggatcagatt
    29341 gactatagcg gtgtggaatc atccatgcca ccaaccgcat ttgataatga accttcattg
    29401 catatatggt ttgttgatac agaagctggt gaaaaacatg taactatacc aaaagcaaaa
    29461 acgatcattg cacagcgtat tgcaaaggag attaatactc ttgtacagga aaaaccaata
    29521 cagtatagcg atattgctgt gctggtgagg aaacataagg aagctcgtgg ggtgcaggat
    29581 atattacaac gatatgggat acacagtgta ttgtacagca cagaatcaat atttgaatct
    29641 gatgaagcta tggagctttt gcaggtgatg gaggcaatag taaacccttc atcggcacaa
    29701 aagatattca gggcactgat aacaagcttg tatggatata cagcacagca tattgttgca
    29761 ttaaacgatg atagtaaact tgatagcatt acaatgcgct ttatggatta ttataatgca
    29821 tggaagagca aaggatttat tcagtgcttt cagctcatga tgcacgatga cacaatcaac
    29881 aggcatttta atgaagatat tgatactgtt tcatcacgtt tattgcatta tcctgatggc
    29941 gagcgtgtta taaccaatct ttatcactgt gccgaaattt tgtcacaggc tgaaaccaca
    30001 aagaagctgg atgcactttc gctggtggaa tggtttaaaa aatctatact cataaagccc
    30061 gatgttgaag aaaacaaatt acgattagaa tccgatgcca atgcggtaaa gatagtaacc
    30121 gtacatttta gcaaaggatt ggaatatcct gtggtgtttt gcccatacct ttttaatgtg
    30181 ggcatgctgg atgatattat tgtttttcat ggtggcgata gtaactgtac aacaaatgtt
    30241 tgccttaaat tgaatgctga taaggataaa accaatggtg acaacctgta tggaatagat
    30301 tataaacact atgtcaatca ggcattgaaa gagcagcttg ccgaagatat acgcgtggca
    30361 tacgttgcaa tgacgcgggc aaagtaccgt tgttatgttg ggtggggtaa tattaataaa
    30421 actaacagat ctgcgttaag ttatcttttc cactatagat ttgcaaataa taccactatt
    30481 gacaattttc ttgataacaa ttttaataat aatgcagctt caaatgaatt aaaagatgga
    30541 ttaaaaaaac ttgttaacct ttctgatgga tccattgcgc ttgaggtttt taacactgta
    30601 cctctggata aagccatggt aacgcaggtt atggatacta tcgtggttga gccagagcgc
    30661 atacaaaaag atatttcacc tggctggcag ttgaccagct ataccgcact atcgtacaca
    30721 aaagaagatg aaaaaggtga tgaggtatat gcttcagtgc cgcacgatga acagcatgaa
    30781 ccaacaatct ttactttccc ggcaggcagt gtaacggggc tttgtattca cagtatcttt
    30841 gaagaggttg acttcgcatc cggtgaagat gcaatgcgca ccatagttgc tgaaaaattg
    30901 caacactatc ggtttgatgt aagctggacg gatacaattg taactatggt acgcaatgtt
    30961 ctttccgttg agctcaatac tgctgttcct ttaaacaggg taacacggga aaatagacag
    31021 cacgagattg aattttatta tcctgttgac aggcttaatc gtgatgaatg tgtgcaatta
    31081 ttttcaatga ataacctatt tgatacacac tatggcagtg cattgcagcg cctttccttt
    31141 gatcatattc agggatttat gcatggatat attgatatgg tattccggta taatgacgca
    31201 tattatattg ttgactggaa aacaaatcag cttggctata caatggacga ctatcatcgt
    31261 agcaaattaa aaagtgccat gatagatcat ttttattttt tacagtatca catctatgct
    31321 gttgctcttc acagacatct ggcaatgctg ttgggaaaaa gttataatta caacaaacat
    31381 tttggcgggg tattctacat ctttgtgcgt ggcgtcaatg ctggtaaagg tggtgactat
    31441 ggcatctttt atgataagcc agattgtcaa tttattgaga aactcaataa ttatttacat
    31501 gcggtgaaag tatgatgcag caacaatcat acactgattc aatccattac tattttgccg
    31561 atttcatctg caataaatat aattgtaaca gtgaagtggt aaaggcatta ttattttgtg
    31621 taagctatat gacatcatac gggcatagct gcctttcact ggcaactatc gcccataaaa
    31681 aactggatga gatagttttt gaagattata cctccatccc cgagctgaca tttcctgatg
    31741 taacatcatt gcatactgta cttatggagt gtcatgcagt aaaagctgac gatgacgatg
    31801 ctataaagcc aattgtatat gataatggca attgctattt atatagatac tttaattatg
    31861 aagaaattat agcacagtgg tttttgaata gtataagcaa aagtcatgaa atagatatgc
    31921 agcaggtaaa cgccgtactt gaaacatttg aacaaaaggg gatgcatttt gcttctttac
    31981 aaaagattgc acagcttgcg gcattgcacc atacgttttg tgttatttct ggtggccccg
    32041 gtacaggcaa aacaacaacc gtagcaggca ttgtatatca gcttcttacg gcacataata
    32101 cattgtccat tgcattgtgt gcgccaacgg ggaaagctgc atcacgcctt aaagaatcaa
    32161 tgaaacaatt taaggataag tattgtgatg atgctataaa agatatattc ccggttgaat
    32221 cgtcaacaat acataggttg ctgggctata aaaaaggcgc attagaattt tattataatg
    32281 aagataatcg tcttccctat gatgttgtga ttgttgatga atcatccatg gtggatattg
    32341 ct
//
LOCUS       DAOOJH010000020        30914 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1573_length_30914_cov_10.137270, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000020 DAOOJH010000000
VERSION     DAOOJH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30914
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_30914_cov_10.137270"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLJ75_04535"
     CDS             complement(<1..183)
                     /locus_tag="PLJ75_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04514.1"
                     /translation="MLITIISIAIGNRTLLYCNKNKKYNYSVFTLLAGVLLVVVPSDI
                     GSNVCHFPYKKKRCMPC"
     gene            275..1018
                     /locus_tag="PLJ75_04540"
     CDS             275..1018
                     /locus_tag="PLJ75_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPD04515.1"
                     /translation="MFTLVSTKSVASDHYLLTLQSEVLASQPGQFINIKVTNNTDPLI
                     RRPFSIFDHSGTNLQIVVRVVGKATRQIAALHPGAIDCIGPSGKGFAIVTGKKALLIG
                     GGVGNAPLFYCAKKLREHNSTVTYIYGATSQNTIYMLDDYKNISNELIVVTDDGSAGV
                     EGNPVDIARAIIKTNSFDMIYTCGPEIMMQRITQELSPIPIQASLERYFGCGIGLCSG
                     CAVATKAGYKRACVDGPVFDGYDVLWDKP"
     gene            complement(1032..1595)
                     /locus_tag="PLJ75_04545"
     CDS             complement(1032..1595)
                     /locus_tag="PLJ75_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HPD04516.1"
                     /translation="MDLKNFYIEAQDTALFVIDVQDSLFNSMEYEMRRKVAKNVAILI
                     ELAKQYNLPIVVTEQYRKGLGSTIKEVMDKLEGVVPFDKLTFDCMKDDSIREQVAGLN
                     KRNLIVCGIETHICVLTTSLSLMKAGYNVVIASDAVCSRRTHEWDMAIQTLRQAGAVV
                     YPTETIAFMMLEKAGTEIFKKLSPLFK"
     gene            complement(1613..4135)
                     /locus_tag="PLJ75_04550"
     CDS             complement(1613..4135)
                     /locus_tag="PLJ75_04550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPD04517.1"
                     /translation="MQRYNLNNRKFSKKGYAGTGGFFTRIVHFFEDVLYALELRFGNI
                     RPIIIGLIAGAIIAFCAILVIDFYKVRALAHYTPYQTTKIYDKNNVLVAEFFKEKREV
                     VPLKKIPPHLIQAFIAMEDNEFYDHFGINPKGIVRAFFVNIVSGRVKQGGSTITQQLS
                     KILLTSGERSIYRKVKEAFISIMIEMFYTKDEIMEMYLNQIFLGHGAYGVESASKFYF
                     GKHVWEINIAESSLLATLPSAPNILSPIRNPRLSMERHRIALAKMVDMGFITVAQAEK
                     AYLDFWPDYLAYINDLPPTMTTWSSRVNKAPWFTEHIRRILVNKFGEKAVYEDGLAVY
                     TTVDVDKQQCAQSLLKDAVEKQTTVSRGLAFDNEEYITNRYADEVNLLTIMFGLPAIK
                     SAGTAQKRTFNRYMKDGLLHEMQMINLITGLDTIDDVLQQYFLGSYDQKEFMNVEGCL
                     VSINPGNGYIEAMVGGSDFTSTNQLNRVMQSMRQAGSAIKPLLYIAAIDSKKFTAATT
                     IMDSPILYLDMEGGDWIPENYEGEYYGLVTLRRALSLSINVVSVRIADTLGIDTVLNN
                     YAKLLKISRNEMKNRVPRNFSIALGSFEVSPLELARAYAIIANGGKNVIPYSIRYVQD
                     KTGKIIYNQEEEIMAELAEQEKDGSIQIVSPDAARIVVSLMQSVISSGTGRGAAIGRP
                     VAGKTGTTSNYRDAWFVGFTPQLVTGIWMGYDGRGLSLGPGQSGGAVVAPVWGRYMSC
                     ALQKEPVLDFAPYGNIAVTKVCAESGLLPSYYCKKVIDELFIPGTEPQEECAKCAGGI
                     SLPTTKIPHINVSEKQKEQIMKKMKRKDTEDILDSIENTLKQ"
     gene            4328..5158
                     /gene="dapF"
                     /locus_tag="PLJ75_04555"
     CDS             4328..5158
                     /gene="dapF"
                     /locus_tag="PLJ75_04555"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPD04518.1"
                     /translation="MPFTKLHGIGNDYIYINAIKHSVKNPKKLAIAMSNRNFGVGSDG
                     LILILPSDKADFTMRMFNADGSEAEMCGNGIRGFAKYVYDHNLTKKTTIAIETLAGIK
                     QVRCTLKNGKVHTVTVDMGEPILLRDKIPMIGNPGTVIEEDLHVDGVKFSITAVSMGN
                     PHVVIYVEDVKNFPVEKYGPMIENHELFPKRTNVEFVQIVTGKEVIQRTWERGSGETL
                     ACGTGASAVTVAGVLTEKTDRRLKVHLKGGNLAIEWNNKDNHVYLAGPAEEVFEGVWP
                     "
     gene            complement(5184..5843)
                     /locus_tag="PLJ75_04560"
     CDS             complement(5184..5843)
                     /locus_tag="PLJ75_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04519.1"
                     /translation="MRLGVKNFITRHKLDTLPGILKMKLLTYYQAIAAGSNKLMDLCL
                     YWICNPPLDTKKRYRALLLAISVVCIVDYCAISVLDDKNLFDIYPSLPALDTRKTIDI
                     FVADIDAKSILKENRRVLLPQDDEGKVNRLLKLVAEGSEFENTRAIAPVDIILRHIWF
                     YDTTCIIDCELSTIEGHVPSIADSFQAFTKALTQTICHNVKGVTKVEVLEHGIYGKNL
                     W"
     gene            complement(5852..6988)
                     /locus_tag="PLJ75_04565"
     CDS             complement(5852..6988)
                     /locus_tag="PLJ75_04565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPD04520.1"
                     /translation="MLLTVCLLPLFPQDSIVIPVKIINSNTYVSEYDIRTILPLQSTF
                     DIVLQRGRIVNKERYALYHVGLAIALVNDSLVRSDYPVMRRHGEVMIPLEIAFTMLTA
                     LLPEYEVDIKDNAITLKKIPEYVKEKEKKTPVTKVSKDAIGFIVVDAGHGGKDPGAIG
                     KGGIKEKHITLQIAKEVKLVLQQRLPGIEVILTRTTDRFIELGERTEIANTMLKKGVN
                     GLFISIHCNAAVVSKISGFETYYLSQNPSNEEARTTSVLENNVIIFENPEKRKRYGDV
                     EMIEALMITTQIQKESAMLAASVQRYLDKTIHEFKSKGVKKADFFVLRGCLMPSVLVE
                     VGYITNPKEKKYLIDKNYQKRLAQGITAGIVAFINEYNRNIANY"
     gene            complement(7042..7644)
                     /locus_tag="PLJ75_04570"
     CDS             complement(7042..7644)
                     /locus_tag="PLJ75_04570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPD04521.1"
                     /translation="MKMSDLFEGRIATWSNLISLLRVVVSPVVGILLYYEKATADTVY
                     CSIAVTVYVLIALSDIVDGYLARYLHQVSRIGQFLDPIADKLTIFILSLILYIFKGFP
                     LWLVIVIVVRDIGHIAAGAILFCTHDIQVRPSNAGKCMIFILGMTGFIYIINLSYSVM
                     GISVKHIFVALTTALIVLSTVVHWNIYSKIYFTSKSVSSK"
     gene            8006..8761
                     /locus_tag="PLJ75_04575"
     CDS             8006..8761
                     /locus_tag="PLJ75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPD04522.1"
                     /translation="MKVRLIICVIAASFFLVQCSKSGEELKYTAKLSKAVIINDNTSV
                     RIDPYIFSSRVSTVDKGTIVTVTDVSKEKQWVGGQIDYWYKVMLPNTIRGWVFGSSLA
                     LQIDASEAAIERYVDTLWKKEADTVRREIAGKWWSVNKSGDFTEHALELYPDGSYKSY
                     RKGSNGIEGSYSLNFRTSEIVFLDSTSFGKNLYFAKRGNAYKLTDAINDPGIEFKKIA
                     EFKDSMSDIEPESKVPNSPEGATPDDTATDTNQ"
     gene            8733..9470
                     /locus_tag="PLJ75_04580"
     CDS             8733..9470
                     /locus_tag="PLJ75_04580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPD04523.1"
                     /translation="MIQQRIRINKFLSLCGLGSRRKTEDLITGGLITVNGTVMRDLSY
                     QVDSAADIVMYKKQVLTPVQSFHYLIVNKPKGFITSANDERSRSTVFDLIPQRLKELG
                     IFPVGRLDKDTEGLLLFTNDGDLAYRLTHPRFKIPKTYEVLLDKPLRDDHKYSIEHGI
                     ELYGSITLPAIIKPLNDEWTAVTITITEGKKRQVRLMFTMFGYEIVSLTRIQYGPIAL
                     GNLPEGKYRILTRQEIEQLKSLPKLIS"
     gene            9545..10093
                     /locus_tag="PLJ75_04585"
     CDS             9545..10093
                     /locus_tag="PLJ75_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPD04524.1"
                     /translation="MQNFDFYQNNKSHYNDFVTQDLQHYTIAQNTIPHTHSQRNAHRM
                     LAAIIALIIISFTAGIVVGIKFATGNTRTIIDPQTKIALSNAAQKAKTIMAPQQKPQF
                     SKIEYPFAIKIGNTFNKNEATQLAALLSKTGQRIIIAKNKEHYHIFAGPYKTIDDAKK
                     SLKIIMGQQENGNFKNAIIIKR"
     gene            10100..10573
                     /locus_tag="PLJ75_04590"
     CDS             10100..10573
                     /locus_tag="PLJ75_04590"
                     /inference="COORDINATES: protein motif:HMM:NF005840.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPD04525.1"
                     /translation="MNFTIRKANNNDINNILNILSPYVKQGILLPRNTDDIANNLSTF
                     SVAVTPKKLVGVISFYKYNNKLYEIRSLAVNGRYHHHGIGKQLVEYAINDIVQQSKEA
                     VIFCLTYNPAFFKKTGFTEVEKNTLPHKIWKDCQYCKHRDNCTESAMIYKAHIKE"
     gene            complement(10563..11411)
                     /locus_tag="PLJ75_04595"
     CDS             complement(10563..11411)
                     /locus_tag="PLJ75_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04526.1"
                     /translation="MKHAIVILLLTVSISVQAQMPDWAMMKDREGNTYYFDKSGKIYT
                     DENIEPAYKAVANDELEYYTSKVKTLWKAGYRKQALTIAKSIMALQETSQHVINGKKD
                     IAAFLRENQMKHGNRFVDISREAWLYLTREGNRMYLFNDKVPYIVSIAGNAEVISAKS
                     LDSLQYSQTVLRTGIRFTQTKGFDAIMTIAAESFAGTIESITVLQQHWKNVALDTFQR
                     TLIKSNDSMALYSFITGDALQLVGLELLIIKENRGYMVRIFTTQERFNQYKGEISAII
                     DGFSTP"
     gene            complement(11411..12109)
                     /locus_tag="PLJ75_04600"
     CDS             complement(11411..12109)
                     /locus_tag="PLJ75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPD04527.1"
                     /translation="MVTFEDAKTVATEIVNLVNPLSIVVFGSVAREGRGQDLDLFIVV
                     DDGVSSKATVAILQKKLKHYYQKFAIDEFVVNQSVLQEYYNKGSYFVKLIIKEGRSLY
                     MKEAIVQWMKQAEEELNVSLYLLQGKFYKGSCYHAQQAIEKAIKALLLNKGWDLEKIH
                     NVNRLMALCNDYTIHISLSDDDIVFIDSIYKGRYPADIGLLPLGEPDEHDAARATTIA
                     TQIVNEMNEYIKQK"
     gene            12545..13528
                     /locus_tag="PLJ75_04605"
     CDS             12545..13528
                     /locus_tag="PLJ75_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD04528.1"
                     /translation="MPDDVYKELSKRLMFENSTLLPQIWKCIANELEAQIIAFLPANA
                     TTIAENFHIPMDKATQILEELFKRGAVFKKAKPDAVEYRPPKHIVQFHDASLLWKNAP
                     SDFKNLWVQFMDTEYPPLLEVVTRVGIPSFMRVIPINATLKPRSEVLIYEDAYSIIEQ
                     ANEIAVVDCVCKLSHGNCSKPLNVCIQINNGAQYSLERGTGRKITKEEARDILKLSEK
                     EGLVHMTENKGFGNAICNCCSCCCEMLRFAGNRATMGVVAKSRFMAQVNENNCNSCDV
                     CTTVCPVHAISIDDIATIDTELCIGCGLCASHCPAQAIELVQTRPREHIPL"
     gene            13675..14304
                     /locus_tag="PLJ75_04610"
     CDS             13675..14304
                     /locus_tag="PLJ75_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04529.1"
                     /translation="MELHNPEATQQALAILRSGDPFQWYVITLLALVVYVYFTEIQNK
                     NWQGIAAGLSLYMVHWFVEIINALIQHFSGHALWTVPTGTAFLLLIGVGVELSFMFSI
                     AGLIFSKVLPKDPRAKILGINNRLFIAIANAAFFSIFEIFLVKTPCFVWVYPWWGALP
                     VFITVYIPFWVVSLYCYDWQLKVQKMVIGSMFVVNAVMLVVFAGFLHWI"
     gene            14661..14951
                     /locus_tag="PLJ75_04615"
     CDS             14661..14951
                     /locus_tag="PLJ75_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD04530.1"
                     /translation="MLFSEEQVGFTSELLKSIAHPIRLKVLCFLMDGEKTVGEIEKEF
                     GSTISNISQHLTILRKMHIIHRRKEANFMYYSIKDERILKLISTLKNLYCGQ"
     gene            14941..16536
                     /locus_tag="PLJ75_04620"
     CDS             14941..16536
                     /locus_tag="PLJ75_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04531.1"
                     /translation="MDNKWRFKLIVIIVFFPACVFALPKTVTLKGLVIFNQEYVIRAA
                     NIDTIPASRFTFEAIAQKITKFYNDIGYTLATVQLEKETPTSIILYVDEGRLNRIIFK
                     RLNTLDTLRIQYDFNLPHRIYHKPTVEMQLTHLKKKYAIKDIVVTIQPSRDYSNSLFQ
                     IDRLREPFNTNKLPMIPVIPITPDPRYDLILTVIPYTAEEIGGIRWGFDTSLRRGLIP
                     YIQYTHNDVFQKHDAFKVGFRTGFMYGINGNFSSPPSNTFNEIKSMYNFKPYFRDLFI
                     PQISSKIYHSKTGRKDLGLDQYNYIMMSGLIEPGFIPIERLKVYPGYGVEKVFIFKVK
                     EDPDSDEVQPDISKKTQVWQFIGIEANLDLIPFSLKQTTKRKLRLTLRRYSSDTHFYM
                     VTIDALFQFEFANYDLFLLQMDYRYTNNNVPFYYEEPVSSKTFKGFMTKDYYSTHIAR
                     TSGEYQVSVYRDFVYMGVYNDITLFKGKHILSGNQFGIAYGLSFHYIFFEQFEFALWW
                     GKDYLFSNQESQFNFKFSLSKKW"
     gene            complement(16616..17422)
                     /locus_tag="PLJ75_04625"
     CDS             complement(16616..17422)
                     /locus_tag="PLJ75_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPD04532.1"
                     /translation="MEFTAPVNNTLFCALDCETTGLNPIIDRIVSIGLIHFTVDRIID
                     SFYTLVNPLKPIPPHVTLLHGITAPMVEEAPTIAELFATIEQFTAKAYCVIHNPRFDL
                     SFIDSAFHQYNKTTPDIKAFDTVHLARKTFKQMQSYNLGKLCSYFGITINAHHALSDA
                     EGSMEIFKRIIEKLNAANWCIRDLERYHGGIISADSLAMRSTRNPIDSRIPLGYRITI
                     IYKDASGNMLERSILPQRYFIIGKNNYIQAYCYLRNEIRYFNTARIVAVH"
     gene            complement(17428..18339)
                     /gene="murB"
                     /locus_tag="PLJ75_04630"
     CDS             complement(17428..18339)
                     /gene="murB"
                     /locus_tag="PLJ75_04630"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPD04533.1"
                     /translation="MCSNDIVQELSHHGRVYTNIPMSKLTTFKVGGPADILIEPYHND
                     TIPSIVTIAKQVEMPVTVIGGGSNIVVGDKGIRGLVLRIGSSDDTPVAIIIQGNIVTV
                     DARSTKKFFVNQALEHGLEGMEFMAGIPGCIGGGIVMNAGTTMGTFSDIISHITFYDV
                     DGTLQTIGNETLFSYRKSMIPDGAIIISAECKLNHARNVEDIKKTIKEILIERKNKHP
                     LDYPSAGSVFKNPDGHSSWKLINDAGLRGYGIGGAKVSQLHTNFIINTGKATALDIKH
                     LVEFIQETIFKLYAIHLEPEIRFIGEF"
     gene            complement(18693..19445)
                     /locus_tag="PLJ75_04635"
     CDS             complement(18693..19445)
                     /locus_tag="PLJ75_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04534.1"
                     /translation="MIVDKITTDSYLTDSMEEMKKAKIRSYFDLISILREHPDWLDEL
                     RKLILTEELINLPKKFDEFVKQYQNFIENEFKPLKQTVQKQGEDIAVLKEDVAVLKED
                     VAVLKQDVGVLKSDVGDLKGDSFERKVRERAPSYFGKIIRKCKLISHIELANILDDAL
                     DTGIIGDDERDDALLCDIVVTGALRSDKERKVLIVAEVSVVVDKQDVERAASRTKVIE
                     KCMGLPGIPVVIGKEHTEGALKRADELQVIVI"
     gene            complement(19684..20403)
                     /locus_tag="PLJ75_04640"
     CDS             complement(19684..20403)
                     /locus_tag="PLJ75_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04535.1"
                     /translation="MALLSVNSISVKYDERRVLDKVGFEINESTITALTGKSGSGKTT
                     LLGVLSGLLKPDEGTVLFMGDNIFTWSDLKRSRFRNRDIGFVFQFFNLFSDISAYDNI
                     AFPAILNKQTRNLRQRIMELARYLQISDILERNPTTLSGGERQRVAIARAIINEPKLI
                     LADEPTGNLDTATSHDILKLFAQLRDDKGISIIIATHDERIVKASNVQYHIEGGKISK
                     NNIGKAGQKKKKALLLKTKKR"
     gene            complement(20413..21696)
                     /locus_tag="PLJ75_04645"
     CDS             complement(20413..21696)
                     /locus_tag="PLJ75_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04536.1"
                     /translation="MIQYILRNAIMIFEEIFKNIWKALLSSFGIILLIAFLVLYLSLR
                     ESVTKFVDKRLFGSLDINEMIIEPKAKSGEPTLAYAINSISAEKVKAIRKMPTISSVN
                     AVTCINAATKIRIELFGKSYTPYVPICGVSRQFIKDKVADYKSFTFNPNKEVPVIAPQ
                     VALDMFNNFASINNIPLFTEETLKGLPVTLIIETPKAVEPKKRFKVPAYVHSFSSTFN
                     LTGIVVPDSFINSIAAQYAAETKSAKPILYIRLYAKVKDVKMLPQTTKEIKSMGLAVT
                     SLDEIASKTNQAMSIIDGFSLLVGFFLLLLTVISIFNSYLIIIYNRSYSFSLRRILGV
                     SKLRIIMTFMVEAAFIGGVYGFIGYLAGAYSIQAAGARLSSLIPAFQGIGLEPVGMHI
                     GALMVLFSIAISSLSALIPAIFASNMNLFKALSKQ"
     gene            21886..22674
                     /locus_tag="PLJ75_04650"
     CDS             21886..22674
                     /locus_tag="PLJ75_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04537.1"
                     /translation="MIAILSNKKLGIVLFCMVFLLCCASYMPVEKIYEDSKIVKIILR
                     DKKTITGWYIVNYDATNRISSINKYIPNKNTPIVTIQLQYRGKLLSSYTISSSLPESD
                     TILYQSTHILNYLPTNDLKRVDISFNKPASIKQAKTTFVRIQYTYTNKMLTGLQITGD
                     AFSFKIVCTISYYKGTIDKITLDEKRYSITTKKFEQGLKGYLKMDGAPEYFFDELQNK
                     KVKDKQELDAIFTQTYIKDALYKIKLTKGNEQIIDYFIMFDSSL"
     gene            23417..24418
                     /locus_tag="PLJ75_04655"
     CDS             23417..24418
                     /locus_tag="PLJ75_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPD04538.1"
                     /translation="MPKKMKSQDTAQPKASPPAKRTEAKEKHVYHEKTAKHSKQKKQF
                     QNDSDIIKTNFDDFTDSSISWYLDQINKIPLLSREDEDTLARRARAGDHKAKEELIKA
                     NLRFVVSIAKKYQTSGISLLDLINEGNMGLIKAAERFDPDRGYHFISYAVWWIRQAIL
                     LAISQKTSLIRLPLNRTADIQKIDRVQKTLESKLGREPSPAEIAEELDMDNDEINHLR
                     NITQDFVSLDATIGNSEDNTVLGLVEDLNVESPDKKIFDESLQEALNEVLNTLTPSER
                     EILKLRYGLDGDEPLSLQQIGERFKLSKERIRQIEKKAIRKLRLPSRSQKLKSFLTD"
     gene            24427..25497
                     /locus_tag="PLJ75_04660"
     CDS             24427..25497
                     /locus_tag="PLJ75_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04539.1"
                     /translation="MRYVMTGLLAGMLLAFIPVFDTHTGFVLQPLWHGALHKIQTIEP
                     SSTKIDKLSFAITTDSGYYHLDANGALKRFIAGGDNFIKPTIDGNFYISYKKVGNDIT
                     MYNATNEIFWKIASAEYPYSAPDGSIIVLLTADGSSARIADFNGNIYANTQLNGQFCT
                     AVAFSPQGGFAGIGFIDGRFYIVNNRGQSIYAGQSPQGTIVKGIAIDSTGQFAIVHCG
                     DSNKDSLLCINIARQKTWLHELSTIHHVKTALAVNSSGHALFLDMHSLLAVDIKGKDV
                     TEYPVVKKRYGQSQINNTDDIFTITYPAATGGSVWYIWDWNKGKLYSREFFEEPFLYS
                     IISNNLILLQGQEMLACFAYQQ"
     gene            complement(25445..26317)
                     /locus_tag="PLJ75_04665"
     CDS             complement(25445..26317)
                     /locus_tag="PLJ75_04665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD04540.1"
                     /translation="MPFFSVVIPVYNRAHTITKAVESVLGQSLADYECIVVDDGSTDT
                     TCKELSPYRKKITYIPIPHGGVSTARNAGIAASRGQWIAFLDSDDVWQSKKLEAHFNY
                     IQLHPDMMIHQTDESWIRRGKRVNPMKKHQKKEGYIFNESLHLCLISPSAVVVHRSLF
                     DTVGLFDEKLPVCEDYDLWLRITWHYRAGFIPDKLIVKYGGHADQLSSQYWGMDRFRV
                     YAICKLLACYGSKLQAPQYTMAVEVALSKCRILLHGANKHSNVALAQKVTRVIEWLTA
                     DRQSMQAFPDLVEG"
     gene            complement(26326..27006)
                     /locus_tag="PLJ75_04670"
     CDS             complement(26326..27006)
                     /locus_tag="PLJ75_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPD04541.1"
                     /translation="MKLEKNEIRVAIFILLPLVILLVVIVLKLGYSFATSTIDVYMKV
                     DNISSIKNGTQVKIKGYTVGRVVDIIPVYKPTLHFLALMRIKKEIDLYEDCSAIIMNQ
                     NIIGDTIIEIRNPVRKVRLLQSGAVVEGVEYVSLDTIVQDVHQLLATLTSTVGVIQDI
                     SMESRGNIRSLVANLSKSVETISSVLQDSQKDMLSIMASFRETAKTMNEISIELKKHP
                     IKFLFKSE"
     gene            complement(27017..27784)
                     /locus_tag="PLJ75_04675"
     CDS             complement(27017..27784)
                     /locus_tag="PLJ75_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04542.1"
                     /translation="MKCDETKQLIEVNHVTIEGSKQSLHDVSFTLCLGEDVIFFGAEN
                     SGIDFICPLLAHAVDNYTGDVLYKGQDIKTMDFVEVHNFRKISGYLQRGYALISNMTV
                     YENIALPLRYHTDMPDSEIHELVDSLINYMHLSHCRDVRPVMLTQSEMLRTAYARAIA
                     LNPDMLLVEHPLEGQCLWNAQIFLSALQNRAFTENKTVIVVTYQPSEYIDIATRLIML
                     HDGRVVFDGSKDEFEKTDNDYIKQYVALSPYGPMTLR"
     gene            complement(27891..28688)
                     /locus_tag="PLJ75_04680"
     CDS             complement(27891..28688)
                     /locus_tag="PLJ75_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD04543.1"
                     /translation="MLSVIKKNFFSIIVKINAMVNGFNDFQRYMKSIVLSFRSLRYLK
                     IRSLYTIVINQVKFTGIDALPLVSWIALLLGGTVIIQANTNFPKYGIESFIGNLLVII
                     IARELGPLVTALIVVARSGSAIAAEIATQVQNREIFSLELMGIDTRMYIILPRIVASF
                     TAIFTLIIIFDVIAFFGGYFIALTTVYIPVETFIQTLLDAFTFNDLLVTITKSIIYGI
                     LIPLICCYYGLKPRSSFEIPIYVSKAVIRTLLVIFIINAMISVLFYF"
     gene            28754..30250
                     /locus_tag="PLJ75_04685"
     CDS             28754..30250
                     /locus_tag="PLJ75_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPD04544.1"
                     /translation="MKKYLVISMAYMGKIVKLVIIVLCIILVILQWSRIETTVNLFKG
                     YFLQIKELLITPDTISIIYPAKTLYYKRPYQKSATNIFHCGNLLFYKTTQEESIAAIA
                     NTMIGYTYYYKLYHLQNSIKNINALSTDTVPAGTVLVIPYSLPYISIDLIKKTMTQIP
                     FVKGVYYSGSSLSSQKMMRQLVTLKQAGINSVVFDVKDVNGIINYKSHVPMVVELNTH
                     AKRPVDDIATLIRGFKELDIYAIARIAVFRDHLLATKSPDMAIKSKKTGRVWNDSSKE
                     LWVDPTKQSVWDYNIALACEVAKLGADEVQFDYLRFPTAGDLSDAQYAYETGKKSKIE
                     TITAFLAKATHELHTLNTAVSIDIFGVTAWQKDDDIKKLGQNIALLSNHCDVISPMLY
                     PSHFNDNFDGFANPGDEPYYFVANGNKKIKAIVPTTLIRPWLQAFKWRVSNYDENYIL
                     AQIQACIDTNVYGYLFWNASNDYTVVQKALMKKNNGNATSKLTKENHK"
     gene            30247..>30914
                     /locus_tag="PLJ75_04690"
     CDS             30247..>30914
                     /locus_tag="PLJ75_04690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04545.1"
                     /translation="MIVKDKHFFNDYIKVWDNGAVKDAPFITLGLEAVLTDYYGNLLD
                     APPPFAVKTDERILDLGCGWGRVLKPVRSITRNAVGLDISQQMLIAAKNHCNYHHLPT
                     PLVGGDGTSLPFKDNTFDTVYSLLVLQHLSKDNGIKVLNEIYRVLKPGGAAYIRVPSR
                     FAPENLLMSFLQVISINVFRYKDPIRMRFYRIGEIKKICNSLFSDVEISAHEFRPPWN
                     FHTP"
BASE COUNT         9796 a         6161 c         5464 g         9493 t
ORIGIN      
        1 gcagggcata caccttttct ttttataagg aaaatggcat acattgctgc caatatcact
       61 ggggaccacc accagaagta ctccagcaag caatgtaaat actgaataat tatatttttt
      121 atttttatta caataaagta atgtacgatt accaatcgct atgcttataa tagttataag
      181 cattgtttaa tttcacacaa ttctttaaaa aatagtattg caaattgcac tcatgctata
      241 gtgtattgta ctatagcacc tgagggtaaa aattatgttt acattagtat ccacaaaatc
      301 tgttgcaagt gaccattacc tgctaacatt gcaatcggag gtactcgcaa gtcagccagg
      361 tcaatttatt aatattaagg ttaccaataa tactgacccg cttattcgac gtccattctc
      421 aatctttgat cattcgggta ccaatcttca aattgttgtt cgtgttgttg ggaaagctac
      481 caggcagatt gcagcgctgc accccggagc aattgattgc attggccctt caggtaaagg
      541 ttttgcgata gttaccggta aaaaagcttt gctgatcgga ggtggcgttg gcaatgctcc
      601 tcttttttat tgtgcaaaga agctgcgaga acacaactca acggtaacct atatatatgg
      661 cgcaacttca caaaacacta tctatatgct ggacgattat aaaaatattt ccaacgaact
      721 catcgttgtc actgatgatg ggagcgctgg tgtggaaggc aatccggttg atatagccag
      781 agctatcatt aaaacaaaca gctttgatat gatttatacc tgtggccctg agattatgat
      841 gcaaaggatt acacaggaac tatcgccaat acccatacag gcttcactgg agcgctattt
      901 tggatgcgga ataggtttgt gttccgggtg tgctgttgcc actaaagctg gatacaaacg
      961 tgcatgtgtt gacgggcctg tatttgatgg gtatgatgta ctgtgggata aaccttaatc
     1021 attgaaaaaa gctatttaaa caatggcgac aattttttaa agatttcagt tcctgctttt
     1081 tccagcatca taaacgctat tgtctcagtt ggatacacaa cagcgcctgc ctgtcgcaat
     1141 gtctgtattg ccatgtccca ttcatgggtt cgtcgtgaac aaacagcatc agatgctata
     1201 acaacattat atcctgcttt cataagcgaa agcgatgttg tcaaaacaca gatatgtgtt
     1261 tcaataccac aaactatgag attcctttta ttaaggcctg cgacctgttc cctgatacta
     1321 tcatctttca tacaatcaaa tgtcagttta tcaaaaggaa ctactccttc cagcttatcc
     1381 ataacctctt ttatggttga acctaatcct ttacgatatt gctcggtcac aactataggg
     1441 aggttatact gttttgcaag ctctatcagg attgccacat tctttgccac cttcctgcgc
     1501 atttcatatt ccattgagtt aaataaacta tcctgtacat caatgacaaa cagtgctgta
     1561 tcctgggctt caatataaaa attttttaaa tccatagcaa tcccttaatc ctttattgtt
     1621 tcaaagtatt ttctattgag tcaagtatat cctcggtatc cttccttttc atttttttca
     1681 ttatctgctc tttttgcttt tcagatacat tgatatgtgg tattttggtt gttggcagtg
     1741 aaatcccacc tgcacatttt gcacattctt cctgcggttc ggttcctggt ataaaaagtt
     1801 catctatcac ctttttacaa taataggaag gcaaaagtcc tgactcagca cacacttttg
     1861 ttacagcaat attgccatac ggcgcaaaat caagcaccgg ttccttttgc agtgcacagc
     1921 tcatataccg tccccacacc ggagcaacaa ctgcaccccc cgattgtcct ggcccaagcg
     1981 ataatcccct gccatcatat cccatccata ttcctgttac aagctgcggt gtaaacccaa
     2041 caaaccatgc gtctcgatag ttactggtag tacctgtttt gccggcaacg ggtctgccta
     2101 tggcagcgcc tcttcccgta cctgatgata taacactctg catcaggctt accactatcc
     2161 gtgccgcatc aggagaaact atctgtatac tgccatcctt ttcctgctct gccagctctg
     2221 ccattatctc ctcttcctga ttatagatga tcttccctgt tttatcctgc acatagcgta
     2281 tggagtacgg aataacattt ttgccgccat ttgcaatgat ggcataggca cgggcaagct
     2341 caagcggtga aacctcaaaa gaacccaatg caatggaaaa attcctggga acgcggtttt
     2401 tcatctcatt acgtgaaatc tttaaaagtt ttgcataatt gttgagcacg gtatcaatac
     2461 caagtgtatc ggctatacgc accgaaacaa cattgatgga taaggataaa gccctgcgta
     2521 gagtaaccag cccataatat tcgccttcat aattttcagg aatccaatcg ccgccttcca
     2581 tgtcaagata taatataggc gagtccataa tggtagtggc tgcagtaaat ttttttgaat
     2641 caatagcagc tatatataaa agaggcttta ttgcagaacc agcctgacgc atggattgca
     2701 taacacggtt aagctgattg gtgctggtaa aatcactgcc gccaaccatc gcttcaatat
     2761 agccattgcc tggatttatt gagacaagac aaccctcaac gttcataaac tctttctgat
     2821 cataactgcc taaaaaatac tgttgcaata catcatcaat agtatcaagc ccggtaataa
     2881 gatttatcat ctgcatctca tgaagcagcc catccttcat ataccggtta aacgtacgtt
     2941 tttgagctgt accggcactt tttatagcag gcaacccaaa cattatggtc aataaattga
     3001 cttcatcagc atagcgattg gtgatatact cttcattatc aaatgccagc ccgcgcgaaa
     3061 cagtagtttg tttttccact gcatctttca ataaactttg tgcacattgt tgtttatcga
     3121 catcaaccgt tgtatacaca gccagcccat cttcgtatac tgctttttca ccaaacttat
     3181 taaccagaat acggcgtata tgctcagtaa accatggtgc cttgttaaca cgtgatgacc
     3241 atgtagtcat tgtaggtggc aggtcattta tatatgctaa ataatcaggc cagaaatcaa
     3301 ggtatgcctt ctctgcctgt gcaacagtta taaaacccat atccaccatt ttagctaatg
     3361 ctatacggtg acgctccatt gaaagacgcg gatttcgtat gggcgatagt atattgggag
     3421 ctgaaggcag tgttgcaagc aaggagcttt cggcaatatt gatctcccag acatgcttgc
     3481 caaaataaaa ttttgacgct gactcaacgc cataagcacc atgccctaaa aatatttgat
     3541 tgagatacat ctccataatc tcatctttag tatagaacat ctcaatcata atagagataa
     3601 aagcttcctt aaccttgcga tagatagagc gctcacccga ggtgagcaat atcttggata
     3661 actgctgcgt aatggtgctc ccaccctgtt ttacacgccc tgaaactatg ttcacaaaaa
     3721 acgctcgcac aatacccttt ggattgatac caaaatgatc ataaaactcg ttatcctcca
     3781 ttgcaataaa tgcctgtatc agatgtggtg gaattttttt aagtggtacc acctcgcgtt
     3841 tttctttgaa aaactccgct acaagaacat tatttttatc ataaatcttt gttgtttgat
     3901 agggggtata gtgagccagg gctcgtacct tataaaaatc tataacaaga atggcacaaa
     3961 atgcaataat ggcaccagca ataagcccaa taattatcgg gcggatattg ccaaatcgca
     4021 gttcaagagc atacagcaca tcttcaaaaa aatgaactat tcgggtaaaa aagccgcctg
     4081 tacctgcata ccccttttta ctgaacttcc tgttgtttaa attatatcgt tgcatgcctt
     4141 tcaatactct aatttcatcg ttaccagtta cggaaatatc tgaaatgtaa gtcaactaaa
     4201 tattgcaaca actatttttt tatttaccaa taactatcgc cacgctattt aaacattttc
     4261 ttgacacata acgccttgta ttatatctat tacaatgaca gtgcatagaa tatagaggtt
     4321 acctatgata ccatttacaa aattacacgg tattggcaat gattatatct acataaatgc
     4381 aataaaacac tcggttaaaa atccaaaaaa attagctata gccatgagta accgtaactt
     4441 cggggtaggt tctgacggac ttattttgat actgccatca gataaggctg attttaccat
     4501 gcgcatgttc aatgcagatg gcagtgaagc cgagatgtgt ggaaacggca tccgtggatt
     4561 tgccaagtat gtgtacgatc acaatttaac aaaaaaaaca acaatagcca ttgaaacgtt
     4621 agctggaata aaacaggtca ggtgtacttt aaaaaatgga aaggtacaca ccgtaaccgt
     4681 agatatgggc gaacccattc tattaagaga taagataccc atgattggta atcccggaac
     4741 cgtaatagag gaagaccttc acgttgatgg cgtaaagttt tccattactg ctgtctctat
     4801 gggtaatcct catgttgtca tctatgtgga ggatgttaaa aacttccctg ttgaaaaata
     4861 tggtcctatg attgagaacc atgaattgtt cccaaaacga accaacgttg aatttgtaca
     4921 gatagtaacc gggaaagagg ttatacagcg aacctgggag cggggaagcg gcgaaacgtt
     4981 agcctgcggc acaggcgcat cagcggttac cgttgccggt gtgttaacag aaaaaactga
     5041 tagaaggtta aaagtacatc tcaaaggcgg caaccttgca atagagtgga ataataagga
     5101 taatcacgtc tatttagcag gccctgctga agaggtgttt gaaggtgtat ggccgtgata
     5161 ccacgtatac aggcttattt ctattaccag agattttttc cataaatacc atgttcaagc
     5221 acttcaactt tcgttacacc tttcacattg tgacatatcg tttgggttaa ggcttttgta
     5281 aacgcttgga aagaatcagc tatagagggc acatgtcctt ctattgtgga cagctcacag
     5341 tcaataatac aggtggtatc ataaaaccat atatggcgca ggattatatc tacaggagct
     5401 atcgccctgg tattctcaaa ttcagagcct tcggctacca gctttaaaag cctgttaact
     5461 ttgccttcat catcctgagg aagtaacacc ctcctatttt cttttaatat tgattttgca
     5521 tcaatatcag caacaaaaat atcaatggtt tttcgggtat ccagcgcagg caatgatgga
     5581 tagatatcaa ataaattttt atcatcaaga actgaaattg cgcagtagtc aactatgcat
     5641 acaacactta ttgccagaag caatgctcta taccgttttt ttgtatccag cggtggatta
     5701 caaatccagt ataaacatag atccataagt ttattacttc cggctgctat tgcttgatag
     5761 tacgttaaca gtttcatttt tagtataccg ggcaacgtat caagcttgtg tctggttata
     5821 aaatttttaa cacctaatct cattatattc ctcaataatt ggctatattc ctgttgtatt
     5881 cgtttataaa tgcaacaatg cctgctgtta tcccctgtgc aagccttttt tgatagtttt
     5941 tatctattaa atattttttt tcttttgggt tggtaatata ccccacctca acaagtacgg
     6001 acggcataag acacccccgc agtacaaaga agtcagcttt ttttactcct tttgatttaa
     6061 attcatgtat tgttttgtca agatatctct gaactgatgc tgcaagcatc gcactttctt
     6121 tttgtatctg tgttgtaatc atgagcgctt caatcatctc aacatcaccg tatcgcttgc
     6181 gcttttcagg attttcaaat ataataacgt tgttttcaag caccgaggtt gtacgtgcct
     6241 cttcatttga aggattttgt gaaaggtaat atgtctcaaa acctgatatc ttactcacca
     6301 ccgctgcatt gcaatgtata cttataaaaa gcccattaac accttttttt aacattgtat
     6361 ttgctatctc tgtcctctca cccaattcta taaatctatc tgtagtccgg gtcaagatta
     6421 cctctattcc gggtaacctt tgctgcaaaa ccagctttac ctctttagca atctgcagcg
     6481 ttatgtgctt ttcttttatg ccacctttac ctatagcacc ggggtccttc cctccatggc
     6541 ctgcatcaac cacaataaaa ccaatagcat cctttgatac ttttgttacg ggtgtttttt
     6601 tttctttttc ttttacgtac tcaggtatct ttttgagggt gattgcattg tcttttatat
     6661 ctacttcata ctcgggaagc aaagctgtca gcatggtgaa ggcaatctcc agcggaatca
     6721 ttacctcacc atgccgcctc ataacaggat agtcactcct taccagagaa tcattcacca
     6781 gagctatcgc cagaccaaca tgatacagtg catacctctc tttattgaca atccttcccc
     6841 gttgtaacac aatatcaaat gtagattgca ggggaagtat cgttcgtatg tcatattcag
     6901 aaacatatgt gttgctgttt ataattttta ccggtatgac aatgctatcc tggggaaaca
     6961 aagggagtaa acatacagta agtaacatga tgatactata acaatggcgg gatagatttt
     7021 tgttataaaa aagcattctg tttatttaga ggaaacactt ttgcttgtaa agtaaatttt
     7081 agaatatatg ttccagtgaa caaccgttga tagaacaata agcgcagtgg taagtgctac
     7141 aaagatgtgc tttacgctta tccccatgac tgaatatgac aggttaataa tatagataaa
     7201 acccgtcatc cccaaaataa aaatcataca cttcccggca ttgctggggc gcacctgaat
     7261 atcatgggta cagaaaagaa tagctccagc agcaatatgg cctatatccc tgactactat
     7321 gacaatgacc aaccacaatg gaaaaccctt aaaaatatac agtataagcg aaagtatgaa
     7381 tatagtcaat ttatcggcaa tgggatcaag aaattgtcca atgcgggaaa cctgatgcaa
     7441 atagcgtgca agatatccat ctacaatgtc agatagagct atcaatacat acactgtaac
     7501 tgcaattgag cagtatactg tgtcagcagt agccttttca tagtataaca atatcccgac
     7561 aactggcgat actaccacac gcagcaagga gatcagattt gaccatgtag caatacgtcc
     7621 ttcaaaaaga tcactcattt tcattatagt gcaaccattt tcattataat gaaattactt
     7681 attataatta ggcacatgat atgtaataat gcactgcgtc aagctaattt ccttaaatac
     7741 ttttttgcaa tttttttcta taatgagcgt acaaacttta attttttaac agccgtatga
     7801 gctcccaata gcactatccc tgcactacca tggttgtgta cagggaaaaa gcggtttttc
     7861 tttgtgtcca ccataaaacg ttattgacga aataacatgc catgacctat atcattatcg
     7921 ttgttttgta atcatcatag tggcgatagt tacccataaa ctatcgccca aaatatggta
     7981 aaagtattta atcagaggaa tcccaatgaa agtaaggttg attatctgcg ttattgcagc
     8041 atcatttttt ctggtacaat gcagcaaatc tggcgaagag ttgaagtaca cagcaaaact
     8101 atcaaaggct gtcatcatca atgacaatac atcggtacgt atcgacccct atattttttc
     8161 ttcacgcgtc agcaccgttg acaaggggac aatagtaact gtaaccgatg tttcaaaaga
     8221 aaaacaatgg gttggtgggc agatagatta ttggtataag gtcatgttac ccaatactat
     8281 acggggctgg gtatttggga gctcgttagc cttgcagata gatgcttcgg aagcagctat
     8341 agaacgatat gttgacacct tatggaaaaa agaagccgac accgttcgca gggaaatagc
     8401 tggcaagtgg tggagtgtca acaaaagcgg tgatttcacc gagcatgcac ttgagttgta
     8461 tcccgatgga agttataaat catatagaaa agggagcaat ggcattgaag gaagctattc
     8521 acttaatttc agaactagcg aaatagtttt tttagacagt accagttttg gaaaaaatct
     8581 atactttgca aagcgaggta atgcctacaa gcttaccgat gcaataaacg atcctggcat
     8641 tgaatttaaa aagattgctg aattcaaaga ttccatgtct gatattgaac ccgaatcgaa
     8701 agttcccaac tcaccggaag gagcgacacc cgatgataca gcaacggata cgaatcaata
     8761 aattcctctc actttgtgga ttgggatccc gcagaaaaac agaggatttg ataacgggtg
     8821 ggcttataac agttaacggt actgtcatgc gcgatctatc gtatcaggta gatagtgctg
     8881 ctgatatcgt tatgtataaa aaacaggtgc ttactcctgt acaaagtttt cactatctta
     8941 ttgtaaacaa acccaaagga tttattacct ctgcaaacga tgaacgcagc cgttcaacgg
     9001 tatttgattt gataccacag cgcttgaaag aattggggat tttccccgtt ggaaggctgg
     9061 ataaagatac tgaaggattg cttttattta ccaatgatgg cgatttagcc tatagattaa
     9121 ctcatccacg gttcaagata cctaaaacct acgaagtact attagacaaa ccactacggg
     9181 atgatcataa atattctatt gaacatggca tagagctgta tggatcaata acattacctg
     9241 caattattaa accactgaat gatgaatgga ctgcggttac cataaccatc accgaaggga
     9301 aaaagcgtca ggtacgcctt atgtttacca tgtttggtta tgaaattgta tccttaaccc
     9361 gcatacaata cggacccatt gcactgggta accttccaga aggaaaatac agaattttaa
     9421 cccgtcagga aattgaacaa ttgaaatccc tgcccaaatt aatttcttaa tacactattt
     9481 catgaatgac gataaaaata atactacacg tcattgaatc ttaacacata aaggttttgg
     9541 aattatgcaa aattttgatt tctaccaaaa caataaaagt cattataatg attttgtaac
     9601 acaggattta caacattaca ctattgcaca aaatacgata ccccataccc attcacaaag
     9661 aaatgcacac cgtatgctgg ctgctatcat tgcattaatc attatttcat ttacagcagg
     9721 gattgttgtt ggcattaagt ttgctacagg aaataccaga acaataattg acccccaaac
     9781 aaaaatagct ttgagcaacg cagcacaaaa agcaaaaaca ataatggcac cacaacaaaa
     9841 acctcaattt tcaaaaatag agtacccgtt tgcaataaag ataggaaata catttaataa
     9901 aaacgaagct acacaattag ctgctctctt aagtaaaaca ggacagcgca tcattatagc
     9961 aaaaaataaa gaacactatc atatttttgc tggtccatat aaaacaattg atgatgcaaa
    10021 aaaatccttg aaaattataa tggggcagca agaaaatggc aattttaaaa atgccattat
    10081 aataaaacgc taaacttaca tgaacttcac catacgaaaa gcaaacaaca acgatatcaa
    10141 caatatcctg aatatccttt caccgtatgt aaaacaagga atactactcc cacgaaatac
    10201 tgatgatatt gcaaacaatc tttctacctt ttcagttgcc gtaacaccaa aaaagcttgt
    10261 aggcgttata tcattttata aatataacaa caaactttat gagattcgtt cattagctgt
    10321 caatggacga tatcaccatc atggaattgg aaaacagtta gttgagtatg ccatcaatga
    10381 tattgtacag caaagtaaag aagcagttat tttttgttta acctataatc ctgccttttt
    10441 taaaaaaaca ggattcacag aggttgaaaa aaacacattg cctcataaga tatggaagga
    10501 ctgccaatac tgtaaacata gagataactg taccgaatca gcaatgatat ataaagccca
    10561 tattaaggag tagaaaatcc gtcaatgatt gctgatattt cacctttata ttggttaaac
    10621 cgttcttgtg tagtaaaaat cctgaccata taccctctgt tttcttttat tattaaaagc
    10681 tctaatccta ccagttgcaa tgcatcaccg gtaataaatg aatacagagc catgctgtcg
    10741 ttactcttaa tcagtgtacg ttgaaatgta tccaatgcaa catttttcca atgttgttgc
    10801 aatacagtaa tggattcgat ggtacctgca aagctctcgg ccgctattgt cataatggca
    10861 tcaaaccctt ttgtttgagt aaaacgtata cccgtcctca atactgtttg ggagtactgc
    10921 aatgaatcca gtgatttcgc tgaaataacc tctgcattcc ctgcaatcga tacaatatag
    10981 ggcactttat cattaaatag atacatacgg ttaccttccc ttgttaaata taaccatgcc
    11041 tccctgctaa tgtcaacaaa acgattgcca tgcttcatct gattttcacg caaaaatgct
    11101 gcaatgtctt ttttcccatt gataacatgc tgtgaggttt cctgcaatgc catgattgat
    11161 ttagctatcg tcaatgcctg ctttctgtaa cctgctttcc acagtgtttt tacttttgat
    11221 gtatagtatt ccagttcatc gtttgcaaca gctttatagg ccggttcaat attttcatcg
    11281 gtataaattt tcccactttt gtcaaaataa taggtatttc cttctctgtc tttcatcata
    11341 gcccaatcgg gcatctgtgc ttgtacagag atactcacgg taagcagtaa aataacgata
    11401 gcatgtttca ttatttttgt tttatatact cattcatttc attgacaatc tgtgttgcaa
    11461 tagtagtagc tcgtgcagca tcatgttcgt caggctctcc taaaggcaac aaaccaatat
    11521 cagcgggata tcttccctta taaatgctat caataaaaac aatatcatca tcagaaagcg
    11581 aaatgtgtat ggtataatcg ttgcataaag ccatgagacg gttaacattg tgaatctttt
    11641 ctagatccca ccccttattc aaaagaagtg ctttaatagc tttttcaatt gcttgttgtg
    11701 cgtgataaca tgagccttta taaaacttac cctgtagaag atataatgaa acattcaact
    11761 cttcttctgc ctgcttcatc cactggacaa ttgcttcttt catatacagg ctccttcctt
    11821 cttttataat gagctttaca aaatagctgc ccttattata atactcttgc aggactgatt
    11881 ggtttacaac aaattcatca attgcaaatt tttgataata gtgctttagt tttttttgca
    11941 atatggcaac tgttgctttt gatgatacgc catcatcaac aactataaat aaatctaaat
    12001 cctgacctct tccttcacgc gcaacagagc caaataccac tatcgacagt gggtttacta
    12061 aattaacaat ttctgttgct actgtttttg catcctcaaa ggttaccata ccacgttcac
    12121 tcgtttatac aatggtttga tagattcaac aattgcatag cagattacat gaatttagtc
    12181 aagtatgttt atcggtggat tgccaatacc tatcgcccac aataaaaata attgacataa
    12241 agtatttgta atataacatt tacccttaat tgaaatatgg agcaacgttt tcttaaaatt
    12301 ccggatttat agttttatac taataatcta tctcacattg actgaagcac agcagatgaa
    12361 atgggttgta aacctatcac tgctgccgtt ctgaagtccg attcaggatc tcatggtgaa
    12421 aaagagattc cagatcaggt ccggaatgac ggtgtggtgt aattctattt tcgggaacaa
    12481 tcctctcctc gccttacaaa gggcaagggg tgaggttact atttctattt ttggagggta
    12541 tgttatgcct gacgatgtat ataaagaact atcgaaacgg cttatgtttg aaaattctac
    12601 actattgcca cagatatgga aatgtatagc aaatgaatta gaagcgcaga taatagcatt
    12661 tttacctgcc aatgcaacga ccattgctga gaactttcat atacccatgg ataaagctac
    12721 acaaattctt gaggaacttt ttaagcgcgg cgccgtattt aaaaaagcaa aacctgatgc
    12781 agttgaatac cgccctccta aacatattgt tcaatttcat gatgcctcac tgttatggaa
    12841 aaatgctcca tctgacttta aaaatttgtg ggtccagttt atggatactg agtatcctcc
    12901 actactggag gttgtcacca gagttggcat accttcgttt atgcgagtta tccctatcaa
    12961 tgcaacgctt aaaccgcgca gtgaggtttt aatctatgaa gacgcatata gcattattga
    13021 gcaggcaaat gagatagcgg ttgttgattg tgtttgcaaa ttgtcacatg gcaattgcag
    13081 caaaccgttg aatgtctgta tacagattaa caatggtgct caatactcat tagaacgagg
    13141 caccggtaga aaaattacta aagaagaagc ccgtgatata ttaaagctgt ctgaaaaaga
    13201 ggggcttgtg cacatgaccg aaaacaaagg ctttggcaat gcaatctgca attgctgttc
    13261 atgctgttgt gaaatgttgc gctttgccgg gaacagggca acaatgggtg tagttgcaaa
    13321 aagcagattt atggcgcagg ttaatgaaaa caactgcaac tcatgtgacg tttgcacaac
    13381 ggtttgtccg gtacatgcta tcagcataga tgacatcgct accattgata ctgaattatg
    13441 tattggttgc ggattatgtg catcgcattg tccagcacag gcaattgagc ttgtgcaaac
    13501 tcgtccaaga gaacatatac cactgtaata catacaaggg gcacagcttt ttagcatgta
    13561 tatatttcac agtaactatc gcaaaaatgc ctttacattt ttttacaata cttcataaac
    13621 tatattcgtt atactaaaag aacgtgatac atcgttaata agtgaggtga aactatggaa
    13681 ctgcacaacc cggaggctac acagcaagca ttagctattt tacgaagcgg cgatccattt
    13741 caatggtatg tcatcaccct gctggcattg gtagtatatg tctattttac cgaaattcag
    13801 aataaaaact ggcaaggcat tgctgcaggg ctttcgctgt atatggttca ctggtttgtt
    13861 gagatcatca atgcactgat acagcatttt tccgggcatg cgctctggac agtccccacc
    13921 ggcacagcgt ttttacttct tattggtgtt ggcgttgaac tgagctttat gttttcaata
    13981 gcagggctta tcttcagcaa ggtattacca aaagatccca gagcaaaaat tttgggtatc
    14041 aacaatcgtt tattcattgc gattgccaat gcagcattct tctcaatctt tgagatattt
    14101 cttgttaaaa cgccgtgctt tgtatgggta tatccatggt ggggagcgtt gcccgttttc
    14161 ataacagtat atataccatt ctgggtagtg tcattatatt gctatgactg gcagctaaag
    14221 gtgcagaaaa tggtgattgg cagcatgttt gttgtcaatg ctgtcatgct tgttgttttt
    14281 gctggtttcc tacactggat atagttgaaa caataattga ggtgtttttt attgaagttt
    14341 ttattttcgc cgtgcgggca cagtcaggat acctatcgtt aatggtatac gctccaccat
    14401 ttttaaaagg ctataaagca aaggatggcg atagtatatc aggattttac ctgctgaaca
    14461 tactatagct ataccctatc aaaccaaatt gattagcaat agccagagaa atagtataca
    14521 agtataaaaa atattgacaa ttattctttc attgctactt tgtgactata tagtaaaatt
    14581 attgtttaat taagaactta aaagcttgtt tactattgtg acattaatgg cattatatca
    14641 tttgcgtgga ggaattatgc atgctgtttt ctgaagaaca ggttggattt accagtgagc
    14701 ttttaaaatc aattgcccat cccatacggc ttaaagttct atgtttttta atggacggcg
    14761 aaaaaaccgt tggcgaaata gaaaaagaat ttggatcaac tatctccaat atttcccagc
    14821 atcttaccat tcttagaaag atgcacatca ttcataggcg taaagaagca aactttatgt
    14881 attactctat taaagatgaa cgtattttaa agctaatctc aaccttaaaa aatctatact
    14941 gtggacaata aatggcgttt taaactgata gttattattg tatttttccc tgcctgcgta
    15001 tttgctctgc caaaaactgt caccttaaaa ggtctggtga tatttaatca ggaatatgtt
    15061 attcgtgctg ccaatatcga taccatccct gccagcagat ttacttttga agccattgcc
    15121 cagaagatta cgaaatttta taatgatatt ggctatacat tagcaactgt acagttagaa
    15181 aaggaaaccc ctacttctat aatcctctat gttgatgaag gacgcctgaa ccgcatcatt
    15241 tttaaacgtt taaataccct ggatacatta cgcatccaat atgattttaa ccttccccat
    15301 cgtatttatc ataagccaac ggttgaaatg cagcttactc atctaaaaaa gaaatatgca
    15361 attaaagaca ttgtagtaac catacaacct tcacgggatt attcaaattc cctattccag
    15421 attgacaggt tacgagaacc atttaatact aacaagttgc caatgatacc tgttattccc
    15481 attactcctg acccgcgcta tgatttgata ctcacggtta tcccttatac cgctgaagaa
    15541 attggaggta tacgctgggg atttgataca agtttgcggc gtgggcttat cccatacata
    15601 cagtatactc acaatgatgt gtttcaaaaa catgatgcct ttaaagtagg gtttagaacc
    15661 ggttttatgt atggcatcaa tggcaatttt tcttcaccac caagcaatac atttaatgaa
    15721 attaagtcca tgtataattt taagccatat ttcagggatc tctttatccc ccaaatttca
    15781 tcaaaaatat atcactcaaa aacagggcgt aaagatttag ggcttgacca gtacaactat
    15841 atcatgatga gcgggctcat tgagccaggc ttcatcccta ttgaacgttt gaaggtatat
    15901 cccggttatg gtgtagaaaa agtttttatc ttcaaagtaa aagaagaccc ggattctgat
    15961 gaagtacaac ccgatatttc caaaaaaaca caggtttggc aattcattgg aattgaagca
    16021 aatttagacc tcatcccctt ttcattgaaa caaaccacta aaagaaaatt aagattaact
    16081 ctcaggcgtt atagcagcga tactcatttt tatatggtta ctatcgatgc actatttcaa
    16141 tttgaatttg caaactacga tctatttctt ttacagatgg attaccgata cactaacaac
    16201 aatgtgccct tttactatga ggaacctgta tcttcaaaaa catttaaagg cttcatgact
    16261 aaggactatt actcaacaca tattgcacga acatcgggtg aatatcaggt ttcagtatac
    16321 cgtgattttg tttatatggg tgtatacaat gacataacac tgtttaaagg taaacatatt
    16381 ttatcaggca atcagtttgg tattgcctac ggtttaagtt ttcactacat cttctttgaa
    16441 cagtttgaat ttgctctttg gtggggtaaa gactatttat tttcaaatca agagtcccaa
    16501 tttaatttta aattttcgtt atcgaagaaa tggtaatatt taatttaaca atgagtattt
    16561 acagcggcta tctataaaaa aattaataca ttgccgggcg atagttactt acaatctaat
    16621 gcacagccac aatacgtgca gtgttaaaat atcgtatctc attccgcaga tagcaatatg
    16681 cctggatata attatttttt ccaattataa aatatcgttg tggcaatata ctgcgttcaa
    16741 gcatattacc tgaagcatct ttatagataa ttgtaatgcg ataacccaat ggtatacgac
    16801 tatcaatggg attacgagtg ctgcgcatgg caagcgaatc agcacttatg atgccaccat
    16861 gatatcgctc taaatcacgg atacaccaat ttgcagcgtt aagcttttca attattcttt
    16921 taaatatttc catagatcct tcagcatcac ttaaagcatg atgtgcatta atggtaatgc
    16981 caaaatatga gcaaagtttt cctaaattgt agctttgcat ttgcttaaaa gttttacggg
    17041 ctaaatggac agtatcaaaa gcttttatat ctggtgttgt tttattatac tgatgaaaag
    17101 cagaatcaat aaatgaaaga tcaaaacgag gattatggat aacacaatat gctttagcag
    17161 taaactgttc aattgtggca aacaattcag ctattgttgg agcctcctcc accattggag
    17221 cagtaatgcc atgaagcaag gtaacgtgtg gtgggatagg cttgagagga ttcaccagcg
    17281 tataaaaaga atcaataatc ctatcaacag taaaatgaat aagtccaata gacacaatcc
    17341 tgtcaataat aggattaaga ccggttgttt cacagtccaa tgcgcaaaat aatgtattat
    17401 tgacaggtgc cgtaaattcc atagagctta aaattctcca ataaatcgta tttcgggttc
    17461 taaatgaata gcatacagtt taaatatggt ttcctggata aattcaacca gatgctttat
    17521 atcaagtgca gtagctttac cagtattaat aataaaatta gtatgcaact gtgatacttt
    17581 agctccacca atgccgtaac cacgcaatcc agcatcgttg ataagcttcc atgatgaatg
    17641 cccgtcagga tttttaaaaa cagatccggc ggaggggtaa tctaaggggt gtttattttt
    17701 gcgttctata agtatttctt ttattgtttt tttaatatcc tccacatttc tggcatggtt
    17761 aagcttacat tcagcactaa taataatagc tccatcagga atcatggatt ttctgtatga
    17821 aaataatgtt tcattgccaa tggtttgaag cgttccatcc acatcataaa aagtaatatg
    17881 tgaaattata tcagaaaaag tacccatagt ggtgccagca ttcataacaa tgccaccacc
    17941 aatacaaccc ggtatacctg ccatgaattc catcccttca agaccatgtt ccaatgcttg
    18001 attaacaaaa aactttttgg ttgacctggc atcaactgtt acaatgttcc cctgaataat
    18061 aatagcgaca ggggtgtcat cactgctgcc aattcgtaaa accagtcctc gtattccttt
    18121 atcaccaact actatatttg agccaccacc tataacagtc acaggcattt ctacctgctt
    18181 tgcgatagtt actatagaag gtattgtatc attatgatat ggttcaatca aaatatcagc
    18241 ggggccaccc actttaaatg tagttaactt actcattggt atattagtat atactctgcc
    18301 atggtgcgac aactcttgta ctatatcatt tgaacacatt tgttccacag tgttagtacc
    18361 acctcctgaa aattaaaatt atatagtaat ttttttcaat ttcatgcaag caatccttca
    18421 ttatatgcat tatcgcatac cattatctca agcacacagg gttattgtat tttttcaact
    18481 atagttatta agcctgtcgc aaatcatgat tgcagtgctg gtgctaagcc tgccacaata
    18541 cgttcactga ccatgttgaa ttatgttgct aaatccttat cctgaaatga gtttggataa
    18601 tagcattgct atataattat tgaatatacc agtattaaga ttatagcatg aaaatcaatt
    18661 atattttttt taacttcttc ataatatagt atttatataa ctattacctg taattcatca
    18721 gcccttttta gggcaccttc agtatgttcc ttcccaatca caacaggtat tccaggtaaa
    18781 cccatacatt tttcaataac ctttgttctg gatgcagctc tttcaacatc ctgtttatca
    18841 acaacaactg atacttcggc aactatgagt actttccgtt ctttatcact tcgtaatgca
    18901 ccggtaacca ctatatcaca aagcagagca tcatcacgtt catcatcgcc tataataccg
    18961 gtatcaagtg catcatcaag aatatttgca agctctatgt ggcttattag cttacatttt
    19021 cgtattattt ttccaaagta cgatggtgca cgctcacgaa cctttcgttc aaaactgtca
    19081 ccttttaaat caccaacatc tgacttcaaa acgccaacat cttgtttcaa tactgcaaca
    19141 tcttctttta atactgcaac atcttctttt aatactgcaa tatcttcacc ctgtttctgg
    19201 acagtttgtt ttaacggttt aaactcattc tcaataaaat tttgatactg tttgacaaat
    19261 tcatcaaact ttttgggtaa attaataagc tcttctgtca aaataagctt acgtagctca
    19321 tccagccaat caggatgctc tcgtaaaata gatatcagat caaagtatga tcgtatctta
    19381 gcttttttca tctcttccat actgtctgta agatagctat cagttgttat tttgtcaact
    19441 atcattatta cctcctggca cacattaaat atatttatca aggaatctgc atcccttcaa
    19501 taaaaagatt attgtctctc cctcctctta aaataaaatg atgcgctcct atcatgccgt
    19561 attcattcca gcattttatt gttccatagg gagcctgaaa cgactttgta atcaatctat
    19621 tctgatacta tactgccatc ccgaatggtg tttagaacaa ttggaaccat tcattgagaa
    19681 gatctatctt ttttttgttt ttagcaatag tgcctttttc tttttttgac cagcttttcc
    19741 aatattgttt tttgaaattt tgccaccttc aatatgatat tgtacattgc ttgctttaac
    19801 aatacgttca tcatgtgttg caataatgat agatatgcct ttgtcatccc tcagttgtgc
    19861 aaatagtttc agtatatcat gtgaggttgc tgtatccaga ttaccggttg gttcatctgc
    19921 cagtattaat ttgggttcat tgatgatggc tcgggctatc gcaacacgct ggcgctcgcc
    19981 accagataag gttgtgggat ttctttccag gatatctgaa atctgtaaat aacgagccag
    20041 ctccataatc ctttgacgga gattgcgagt ttgtttattt aagatagcag ggaaggcaat
    20101 attatcataa gcagatatat ctgagaacag attgaagaat tggaatacaa atccaatatc
    20161 cctgtttctg aatcgtgaac gtttaaggtc gctccatgta aagatattgt cacccataaa
    20221 aagcacggtg ccttcatctg gttttaaaag tcctgatagt actcctaaca aagttgtttt
    20281 acccgaacct gatttaccag taagcgcagt aatagtactt tcgttaattt caaaaccaac
    20341 cttatcaagg actcttcttt catcatattt gacactaata gagtttaccg acaataatgc
    20401 cacgattcca cctcactgtt tacttaatgc tttaaataaa ttcatattag aagcgaatat
    20461 tgcaggtatg agcgctgaca gtgacgatat tgcaatagaa aacaacacca ttaacgcacc
    20521 tatatgcata ccaacaggtt caagccctat accttgaaat gcaggtataa gcgatgaaag
    20581 ccttgcacct gcagcttgaa ttgaataagc tcctgccaga taacctataa aaccatatac
    20641 ccccccaata aatgcagctt caaccataaa tgtcattata atccttaatt ttgatacccc
    20701 taatattctc cgtaatgaaa agctatagct tcgattatag ataattatca ggtacgaatt
    20761 aaatattgat attactgtca acaataacag gaaaaaacca actaacaggg aaaagccatc
    20821 aatgatactc attgcctgat ttgttttact ggctatctca tcaagcgagg tcaccgcaag
    20881 ccccatggat tttatctctt ttgtagtctg aggaagcatt ttaacatctt taaccttagc
    20941 ataaagcctg atatacaaaa taggctttgc actttttgtt tccgcagcat actgagcagc
    21001 aattgaattt ataaaactat ccggcacaac aatcccggta aggttgaaag tactcgaaaa
    21061 tgaatgaaca tatgctggta ccttaaaacg tttttttggt tccaccgctt ttggcgtttc
    21121 aattatcagc gtaactggca agccttttaa cgtttcttcc gtaaaaaggg ggatgttgtt
    21181 aatagatgca aagttgttaa acatatcaag agccacttga ggtgcaatga ccgggacctc
    21241 tttattggga ttaaatgtaa atgatttata atcagcaact ttgtctttta tgaattgccg
    21301 gcttactcca caaattggca catacggtgt atatgattta ccaaagagtt caatccttat
    21361 ctttgttgca gcattaatac aggttactgc attcaccgaa gatatagtgg gcattttgcg
    21421 tatagctttt accttttcgg cagaaatact gtttattgca tacgctaatg ttggttcacc
    21481 tgatttagct tttggctcaa taatcatttc attaatatct aatgagccaa aaagcctttt
    21541 atcaacaaat ttggttactg actcacgtaa tgaaaggtat aacaccaaaa aagcaatcag
    21601 caatataata ccaaaacttg atagtaaagc tttccagata tttttaaaaa tctcttcaaa
    21661 tatcattata gcattgcgca gtatatactg tatcatagca tcaataatcc ttttcaatta
    21721 aaaataaatt acaatcaccg gtaataaagt attataatca atacctattt ctcatgtcat
    21781 taataaattg aaattaatag cttaaataat aaaaaaatta ttgcattttt atacatcatt
    21841 gccaataatc cttaaaatat tgtaaaccac taatgcaatg ggatcatgat agcaattctg
    21901 tcaaataaaa aattgggaat agtactattc tgcatggtat ttttgctgtg ctgtgcttcc
    21961 tatatgccag ttgaaaaaat ttatgaagac agtaaaattg taaagattat acttagagac
    22021 aaaaaaacaa ttacagggtg gtatattgtt aactacgatg caactaacag gattagcagc
    22081 atcaacaaat atatacccaa taaaaataca ccaattgtta caattcaact gcagtatcga
    22141 ggaaaattat taagcagtta tactatctca tcgtcgttac ccgaaagtga tacaatcctc
    22201 tatcaatcca ctcatatctt gaactatcta cccacaaatg atctaaaacg ggttgatatt
    22261 tcattcaaca agcctgcaag catcaaacag gcaaaaacaa catttgtacg aatacaatac
    22321 acttatacta ataaaatgtt aactggactg cagataactg gtgatgcttt tagctttaaa
    22381 attgtttgca cgataagtta ctataaagga actatcgaca aaattaccct tgatgaaaaa
    22441 cgctatagca taacaactaa aaagtttgaa cagggtttaa aaggttatct aaagatggat
    22501 ggcgccccgg aatatttctt tgatgaatta caaaacaaga aggttaagga taaacaagag
    22561 cttgatgcaa tttttacaca aacctatatt aaagatgcac tgtataaaat aaagttgacc
    22621 aaaggaaatg aacaaattat tgactatttt attatgttcg atagttcctt ataatgagta
    22681 tcacagtatg tattactgct aattatttta tggtagtggt ttccttacta caaagcctga
    22741 cattcttgtt catagttaca atgcttttta cgataaacat gtttttgttt tcaccgctta
    22801 tgcgaactca atttattatt ctcaatcatt cctctctatt cttcctgttt tatattattg
    22861 ccattttttt tcattggggt tcataatgga ttcctgcatc catagggaat aaaaatattt
    22921 tgcgacagtc attctgaagt ctgattcagg acctcgtata attatataga ttccgggtta
    22981 agcccggaat tacattgaga aataattcta tttctggaga agccatacca tggttatcct
    23041 ttcccaatac ttgaatgatg tctgccttat tttcgtgtca ttgccctgac cagatacttt
    23101 tttgatgcat acatcatttt tttggatgac tttttgttca tggtgctata tatgggcata
    23161 gtgcttcata catgcatcct gttttgtttt gtgtgttaca gtcactttac cggttgtatt
    23221 cattatgaca caacattgtt gtattttgca ttgctcttta ttctggaatt tgtcttatag
    23281 tatgccttat agtaaaaaat tttacacatt tcagggaaca ttaattcctt tttttagatt
    23341 gtaaaaatct gctgccaatt ttattattgt actaacatta agtaaaccat ttaatttgca
    23401 caatgtaagg gatggtatgc caaaaaaaat gaaatcgcag gatactgctc aacccaaagc
    23461 ttcaccacca gctaaaagga ccgaagcaaa ggaaaagcat gtatatcatg agaaaacagc
    23521 aaaacacagc aagcaaaaaa agcaatttca aaatgatagt gatataataa aaaccaattt
    23581 tgatgatttt accgattcat cgatatcgtg gtatctggat caaataaata agattccgct
    23641 tctttctcgt gaagatgaag atacgctagc gcgtcgtgcc cgcgctggag accataaagc
    23701 caaagaagaa ttgatcaaag caaatctacg atttgtagta tctatcgcaa aaaaatatca
    23761 aacaagcgga atatcattac ttgaccttat caatgaaggg aatatggggc ttattaaggc
    23821 agctgagcga tttgatcccg acagaggata ccattttatc tcgtatgctg tatggtggat
    23881 acggcaggct atactgcttg ctatatcaca aaaaacatcg ttaatacggc tgcctcttaa
    23941 ccgaacggcg gatatccaga aaattgacag ggttcaaaaa acattagaaa gcaaactggg
    24001 aagagagcca agccctgctg aaatagctga agagctggat atggataatg atgagatcaa
    24061 tcacctgcgt aacattaccc aggattttgt atcacttgat gcaactatag gaaattctga
    24121 agataatact gttttagggc tggtagaaga tttaaacgta gagtcacccg ataaaaagat
    24181 ttttgatgaa tcattgcaag aagctttgaa tgaggtgctt aacaccttaa ctccttcaga
    24241 gcgtgaaata ctgaaactga gatatggact tgatggcgat gaaccattgt cattacagca
    24301 aattggtgag cgctttaagc tatcaaaaga gcgcatacga cagattgaaa agaaagcaat
    24361 acgtaaattg cgtcttccat cacgcagtca aaaattaaaa agctttttaa cggattaata
    24421 aacgctatgc gatatgtaat gacaggctta cttgctggaa tgttattggc atttattcct
    24481 gtttttgaca ctcacacagg ttttgtgctg caaccgctgt ggcatggtgc gttgcacaag
    24541 attcaaacga ttgaaccttc cagtacaaaa attgataaac tttcctttgc aataacaacc
    24601 gatagcggtt actaccatct tgatgcaaac ggtgcgttga aacgttttat cgccggcggt
    24661 gataatttta tcaaaccaac catcgatggc aacttttaca taagctataa aaaggttggc
    24721 aatgacataa ccatgtacaa tgccaccaac gaaatattct ggaagattgc gtcagcggag
    24781 tatccctaca gtgcgccgga tggttcaatc attgtactgc ttaccgctga cggaagttcg
    24841 gcacgtatag ccgatttcaa cgggaatatc tatgctaata cacaattaaa tgggcagttc
    24901 tgtaccgctg ttgcattttc accacaaggc ggctttgccg gcattggatt tattgatggc
    24961 agattttata ttgtcaataa caggggtcag agcatctatg caggacaatc accacaaggc
    25021 actattgtaa agggtattgc cattgatagt accggacagt ttgccattgt ccactgtggt
    25081 gacagcaaca aggattcact gctatgtatt aatatcgcca gacaaaaaac atggttacat
    25141 gagctatcga ccatccacca tgtaaaaaca gcgctggcag tcaattcatc agggcatgct
    25201 ctttttcttg atatgcattc attacttgca gtagatatta agggcaaaga tgttactgaa
    25261 tatccagtag taaaaaagcg atatggtcaa tcacagataa ataataccga tgatatattt
    25321 accataacct accctgctgc aacaggcggc agcgtctggt atatctggga ttggaacaaa
    25381 gggaaactat attcacgcga attttttgag gaaccattcc tctacagtat tatctcaaat
    25441 aatcttatcc ttctacaagg tcaggaaatg cttgcatgct ttgcctatca gcagtaagcc
    25501 attctattac ccgtgtaacc ttttgtgcca gggcaacatt ggaatgcttg tttgcaccat
    25561 gaagcaaaat acgacatttt gaaagagcaa cctccaccgc catcgtgtac tgtggcgctt
    25621 gcagtttgct accatagcat gccagcaatt tacagatagc atataccctg aacctgtcca
    25681 tcccccagta ttgtgatgat agctgatcgg catgccctcc atattttact atcagcttat
    25741 caggaataaa ccctgcacga tagtgccatg ttatccgcaa ccacagatca taatcctcac
    25801 ataccggaag cttttcatca aacagcccaa cagtatcaaa taaacttctg tgtacaacaa
    25861 cagcagaagg gctgataaga cacaaatgca atgactcgtt aaaaatatag ccttctttct
    25921 tctggtgctt cttcatgggg ttaacacgtt taccgcgcct gatccatgac tcatcagttt
    25981 gatgtatcat catgtcaggg tgaagctgta tatagttgaa atgagcctca agtttttttg
    26041 attgccacac atcatctgaa tcaaggaatg ctatccattg cccacgtgaa gctgctatgc
    26101 cagcattgcg tgctgtgctt accccaccat gagggatggg gatataggtt attttttttc
    26161 tatagggcga tagctcctta caggtggtat ccgttgagcc atcatccacc actatgcatt
    26221 cataatcagc aaggctctga cccagtacag attcgacagc ttttgttata gtatgtgctc
    26281 tattgtatac aggaataaca acagaaaaaa atggcatgta cagtattact cgctcttaaa
    26341 taaaaattta atagggtgct ttttaagctc aattgaaatc tcattcatag tttttgccgt
    26401 ttctctaaat gaagccataa tggacagcat atccttctgc gagtcctgca ataccgagct
    26461 tattgtttca acactttttg aaaggtttgc aactaaacta cggatattgc ctctgctctc
    26521 catggaaata tcctggatta ctccaacggt actggtaaga gtagctaaaa gctgatgaac
    26581 atcctgtacg atagtatcaa ggcttacata ttccacacct tcaacaactg cacccgattg
    26641 caataatctc accttcctta cgggattgcg tatttcaatg atggtgtcac caataatatt
    26701 ttgattcatg ataatagcag agcaatcttc ataaagatca atctctttct ttatccgcat
    26761 aagcgctaaa aaatgtaagg tgggtttgta taccggtata atatcaacaa ccctccccac
    26821 cgtatatcct tttatcttta cctgtgttcc attctttatg gacgatatat tatcaacctt
    26881 catgtataca tcaatcgttg aggttgcaaa tgaatagccc agtttgagca caataaccac
    26941 aagtaaaata acaagcggta ataaaataaa aattgcaacg cgtatctcat ttttttccag
    27001 tttcatacat ttactcctac cgcaaagtca ttggaccata aggtgaaagg gcaacatatt
    27061 gtttaatata gtcattatcg gttttttcaa actcatcttt tgaaccatca aatacaaccc
    27121 ttccatcatg aagcatgata agccttgtag caatatcaat atactctgaa ggctgatagg
    27181 taacaactat cactgtttta ttttcagtaa aagccctgtt ttgcaatgcc gaaagaaaga
    27241 tctgtgcatt ccataaacac tgtccttcaa gggggtgctc caccagcaac atatcaggat
    27301 ttagagcaat agccctggca taagcagtac gcaacatttc agactgagtg agcataacag
    27361 ggcgtacatc tctgcaatgc gacaggtgca tatagttaat gagcgaatca accagttcat
    27421 gaatttcaga atctggcata tcagtatggt agcgaagcgg cagggcaata ttttcataca
    27481 ccgtcatatt gcttatcaag gcataccccc gttgtaaata gccagatatc ttcctgaagt
    27541 tatgaacctc aacaaaatcc atggttttaa tatcctgacc tttatataaa acatcaccgg
    27601 tatagttatc aacggcatga gctaacagtg ggcatataaa atcaatgcca ctgttttcgg
    27661 caccaaaaaa aatgacatct tcaccaaggc atagtgtaaa cgacacatca tggaggctct
    27721 gtttgcttcc ttctatggtg acatggttca cttcaatcag ttgctttgtt tcatcacatt
    27781 tcatggtatt ctcatcaaac atagtgcaat aaattcttca acgcctgttg catcattcat
    27841 ctgcaaccca gctcattacc ataactatcg aacaaaacaa tttgttgctt ttaaaaataa
    27901 aacaacaccg atatcatagc attaataata aaaatgacaa gcaatgtacg gataacagcc
    27961 ttagaaacat atatgggaat ctcaaaactt gagcgtggct tcaaaccata atagcaacat
    28021 atcagcggaa ttaaaatacc ataaatgatg ctcttggtga tagttaccag taaatcattg
    28081 aaggtaaatg catcaagcag tgtctgaata aatgtttcaa ccggaatata caccgtggta
    28141 agggctatga agtaaccacc aaaaaatgct atgacatcaa atatgatgat gagcgtaaag
    28201 atagccgtaa acgaagcaac aatgcgcggt aaaataatgt acatgcgcgt atcgatgccc
    28261 ataagctcca gagaaaatat ttccctgttt tgcacctgcg ttgctatctc agccgcaatt
    28321 gctgaaccag agcgggcaac aacaatcaac gccgtaacca gcggtcccag ttcgcgcgct
    28381 atgatgataa caagcaaatt cccaataaag ctttcaatac catatttagg gaaattggtg
    28441 ttagcctgta taataaccgt tcctcccaac aacagcgcta tccatgatac aagcggcaga
    28501 gcatcaatac cggtaaattt tacctgattg atgacaatag tgtacaacga gcgtatcttt
    28561 agataccgta atgagcggaa tgacagcaca atacttttca tatagcgttg aaaatcatta
    28621 aaaccattta ccattgcatt aatttttacg attatactaa aaaaattttt tttaattact
    28681 gacagcatgg tttacatttt ttaagttgaa tttatttaca cacttttaca ttgtgataca
    28741 tttggctgtt aaaatgaaaa aatatcttgt tattagtatg gcatatatgg gaaaaatcgt
    28801 caagcttgtt attattgttt tatgcattat tcttgtcatc ctccagtgga gcaggattga
    28861 aactacagta aatcttttta aaggctactt tttacagata aaagagttgc ttatcacacc
    28921 cgataccatt tcaatcatct accctgcaaa aacattgtac tataaacgtc catatcaaaa
    28981 aagtgcaacc aatatatttc attgcgggaa tctgcttttt tataaaacca ctcaggaaga
    29041 gtcaatagct gcaattgcaa atacaatgat tgggtatacc tactattata agttatatca
    29101 tctacaaaac agtataaaaa atatcaatgc actatctacc gatacagtcc ctgccggcac
    29161 cgttctggta attccctatt ctctgccata tataagcatt gaccttataa aaaaaaccat
    29221 gacgcagata ccctttgtga aaggggtata ctattcaggc tcaagtttat caagtcaaaa
    29281 gatgatgcga cagttagtta ccttaaagca ggccggcata aacagcgtgg tatttgatgt
    29341 aaaggatgta aacggcatta taaattataa aagccatgtc cctatggtgg ttgagcttaa
    29401 cacccatgca aagcgccctg ttgatgatat cgcaaccctt atcagaggat tcaaagagct
    29461 ggacatctat gcaattgccc ggatagcagt atttcgtgat catctgctgg ctacaaaaag
    29521 tcctgatatg gccataaaat caaaaaaaac aggcagagtg tggaacgata gttcaaaaga
    29581 attgtgggtt gaccctacaa agcaaagcgt atgggactat aacattgctc tggcatgtga
    29641 ggttgcaaaa ttaggcgctg atgaggtgca atttgattac ctgcgttttc caacggctgg
    29701 tgatttatct gatgcacagt atgcgtatga aactggcaaa aaatctaaga ttgaaacgat
    29761 aactgcattt ttagcaaaag caacccatga attgcatacg cttaacacgg cagtatctat
    29821 tgatattttt ggtgtaacag catggcaaaa agatgatgat ataaaaaaat taggacaaaa
    29881 tattgcattg ctttctaacc attgcgatgt aatctcacca atgctctatc cttcgcactt
    29941 taatgacaat tttgatggat ttgcaaatcc aggggatgag ccctactact ttgtggcaaa
    30001 tggaaataaa aaaatcaagg cgatagttcc cactacattg atacgcccat ggctgcaggc
    30061 tttcaaatgg cgggtaagca attatgatga aaactatatc cttgctcaga tacaggcatg
    30121 tatcgatacc aatgtttatg gatacctgtt ctggaatgca tcaaacgatt atactgttgt
    30181 acagaaagca ttgatgaaaa aaaataatgg aaacgcaaca agtaagctta caaaggaaaa
    30241 ccataaatga tagttaagga taaacatttt ttcaatgatt atataaaggt atgggataat
    30301 ggtgccgtta aagatgcacc atttattact ttagggcttg aagcagtcct gaccgattac
    30361 tacggtaatt tattagatgc gccaccccct tttgcggtga aaactgatga acgaatactt
    30421 gatttaggat gtggatgggg acgtgtattg aagcctgtac gctcgattac caggaatgcc
    30481 gttgggcttg atatatcaca gcagatgctt atagctgcaa aaaaccactg taactatcac
    30541 caccttccca ctccactggt aggaggtgat ggcacatcat tgccatttaa agataataca
    30601 tttgatacgg tatattctct gttagtgtta cagcatcttt caaaggacaa tggcataaaa
    30661 gtattaaacg agatatacag ggtattaaag ccaggtggag cagcatatat acgtgtacca
    30721 tcgcgatttg ctcctgaaaa tcttttaatg agttttttac aggttatcag cattaatgta
    30781 ttccgctaca aagacccgat ccggatgcgc ttttacagga ttggtgaaat aaaaaaaata
    30841 tgcaatagcc tgttcagcga tgttgaaatt tctgcacatg aattcaggcc gccatggaat
    30901 tttcatacac catg
//
LOCUS       DAOOJH010000021        29967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1647_length_29967_cov_8.760330, whole genome shotgun sequence.
ACCESSION   DAOOJH010000021 DAOOJH010000000
VERSION     DAOOJH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29967
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1647_length_29967_cov_8.760330"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..819
                     /locus_tag="PLJ75_04695"
     CDS             217..819
                     /locus_tag="PLJ75_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013180507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HPD04546.1"
                     /translation="MIIVGITGASGALLGIRCIEELVYHNKQIAVIVSKSAWPIIHHE
                     MQTDCTSIISIVKQRTMQPELLSNIIEYSPDDFFAPIASGSNQFEATIVIPCSMKTVA
                     AIAHGYADNLIHRVCDIALKEKRQLVIVPRETPLSALHLYNLYTLSMYGASVVMPIPA
                     FYNFPETVDDVVNFIIGRVFDQLHIQHNLYRRWHYEEHTR"
     gene            800..1885
                     /locus_tag="PLJ75_04700"
     CDS             800..1885
                     /locus_tag="PLJ75_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPD04547.1"
                     /translation="MKNIHDEKLALIYNKVIAGVPVSNDDAYTMLTTNDIMGLGFIAN
                     TVKKEMHGNIIYYGVNMNLNYTNICLLRCPLCAFSRDKDDHDAYCLSHDEIVHKIKNA
                     ADIDEVHIVGGIHPDLPFSYYEEMIALIKSTRPDIHIVAFTAVEYDYMAKKFNIPLDE
                     LFQRLKKAGIDSIPGGGAEILANHIRTTIAPKKISGQRWLEVMATAHRNGMKSNATML
                     FNHIEDTADIVDHMCKIRDLQNTTGGFKTFVPLVFHEENTGVRARRKDPTGYDIIRIY
                     ATARIYLHNIPHIKALWMYVGEKLAQVLLQCGVDDIGATYYYEKVVHSAGAKTPAWGS
                     EPFLQHLIYDAGFKPVRVNASYEAIKK"
     gene            1898..2707
                     /locus_tag="PLJ75_04705"
     CDS             1898..2707
                     /locus_tag="PLJ75_04705"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HPD04548.1"
                     /translation="MKLGYINYLNCYPFYFNMFEKKPLPDVEVIPAYPSQLNAMIKEG
                     KLAMSPISAAAYAEGYNDLVLLPDFCLSSIGYVRSVILVSKKPIENLNHSTVGLSSAS
                     QTSVVLLKILLQKYYGLVPNYIPSDPYATLHHVDAKLVIGNEAMMHETEPLPYVYDLG
                     DLWLNKTGYPVVFAVFAVRNDALDSNEKTINAVCQSYHTSLLQLKNNAPELIACASLR
                     YPDIAYDIATYYTLLKFEFANKLKEALMFYFTSAHELGLLPYIPSLQFYGR"
     gene            2717..3787
                     /locus_tag="PLJ75_04710"
     CDS             2717..3787
                     /locus_tag="PLJ75_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HPD04549.1"
                     /translation="MNTVDSIINAAYNNKRLEPHEAVLLFDCDVATLGVLANYRREQI
                     VSGNEVGFVIDRIINYSNRCQAMCKFCAFHAQAGKIQSYDLTVDDILQKIDELVHIGG
                     TQVMLQGGLHPDYTLQDYVAMIKTIKQHYPDIYLHSFSPSEIVHIAKRSGSTIDAIID
                     TLQQAGLDSIPGASDLLVDDIRKKVSPRKATVAQWCEVVRSIKKHGMHTTATMTYGMG
                     ETKLQRIEHLDVIRKMQDETGCIMAFIPWSFAPKGTNMDTIIPATGIDYLKTVAISRI
                     FLDNVPYLHAGWLTEGMALAQIALAMGANDMGGILMEEKVVKATGVTTTTNIPQLCDV
                     IINAGKIPVHRDSMYTVLRRMV"
     assembly_gap    3905..4004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4005..4367
                     /locus_tag="PLJ75_04715"
     CDS             <4005..4367
                     /locus_tag="PLJ75_04715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04550.1"
                     /translation="EVFRVLQPGGMFVALEFTRPRNPIVRLIYFLYLYTFLPMIGGII
                     AGNIKAYHYLSKTIHTFIDPPSLLAIQEKHGFVEIKCNSMTLSIVTAYSSIKPKSLSS
                     PLSLQHLNNLQHNRVIIH"
     gene            complement(4441..5091)
                     /locus_tag="PLJ75_04720"
     CDS             complement(4441..5091)
                     /locus_tag="PLJ75_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPD04551.1"
                     /translation="MIFQKNLLKVSCVIVVFISSTLAIAQNNYSNYSFGFSLSGGADD
                     VWHKSGKDDGKDITWNQGIMAGAGLFFENMFTNSFGIHAGINYSYLESELVFQDSTDE
                     KLKSKNHSIIIPLYLLSSFGNTIRIDILYGLSYMHIFHNTMSSNNQHTDGIRYINYNQ
                     FGAGLMLRLVFAFNRFTYFYIAPHGQFYFTNVIEDTSWRDYLYTYQLEIGMIFKTF"
     gene            complement(5120..5470)
                     /locus_tag="PLJ75_04725"
     CDS             complement(5120..5470)
                     /locus_tag="PLJ75_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD04552.1"
                     /translation="MVEQKEIIEDENKIATVIASDISFRGTLKFKSSLKIKGSLEGKV
                     QTDGTLIIGDDARVSAEITAATVSISGKCQGKIKASKLIEIFEHSNMSGDIVTPDLYV
                     ENGAKFNGTCIMPK"
     gene            5866..6264
                     /locus_tag="PLJ75_04730"
     CDS             5866..6264
                     /locus_tag="PLJ75_04730"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HPD04553.1"
                     /translation="MEEILQKANELGLMIKGSDIFKRYEELVKKIEADSNARTLLDEY
                     IKMSEEIYKKESSGGVVEVEEKQKLQDLSQKVSENQLIKEFIATQSYYVNMMMQIQNT
                     ISNPKGDPIGPSKIIKPGAGTTGKIVTGNF"
     gene            complement(6306..7517)
                     /locus_tag="PLJ75_04735"
     CDS             complement(6306..7517)
                     /locus_tag="PLJ75_04735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPD04554.1"
                     /translation="MELAHRLKKIKPSATLAVTALANSLKAQGVDVIGFGSGEPDFDT
                     PQSIKDAAIKALVDGKTKYTPSGGIPELKKAIVQKFKRDNNLEYKLSEVTVNCGGKHS
                     FYNLMQVLLNSGDEVIIPAPYWVSYPDIVLLADGTPVVIHTDELTNFKITPEMLKKHI
                     NKKTKAVVINSPSNPTGSMYTLDELKALGELLKNYDICIITDDIYESIVYNGNKFYNI
                     LNAIPELKSKTMVLNGVSKTYSMTGWRIGYMAGDETIIKQVEMVQSQSVSNPTSIAQW
                     AAVEAIMGDQSFINEMLQAFTRRKEMIVKGLNDINGVECHNPDGAFYVFPNVKKIYQC
                     NGFKHIIDNYKGDSLSSKLSSYLLEEARVAVVPGVAFGSDNNVRLSFATSDKSIIEGL
                     KRIQEAIEKLV"
     gene            7897..8193
                     /locus_tag="PLJ75_04740"
     CDS             7897..8193
                     /locus_tag="PLJ75_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04555.1"
                     /translation="MKRITMIIIITVLSTVLLAKNAFFKGRVIFTNKDYIEVKYGKTE
                     KQFYVNDKSVFKKGGTNVTFADIKPCQIVKVVYAVADKTMYITTCEIIKESDCE"
     gene            8720..9691
                     /locus_tag="PLJ75_04745"
     CDS             8720..9691
                     /locus_tag="PLJ75_04745"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPD04556.1"
                     /translation="MLYINIIKERSVKSLKENLVGALTVMLIVINTLFWACFLFSVAV
                     LKIIPLRFTKKYINTILDTIARLWIYCNNAGLKITRKIVWDVEGDNNLTTNSWYLVIA
                     NHQSWSDIVVLQKILTGKIPFLKFFLKKELIGVPVLGLAWWALDFPFMRRYSQSFLLK
                     YPNLKGKDIEITKKACQKFKDIPVSVMNFVEGTRFTKEKHTKQGSPYSNLLKPKSGGI
                     GFVFSTMGDYITSILDVTIVYPEGNKSFWDFLCGRITMIKVQIDEIPVTEALRGDYID
                     DPVFKRKFQQWLNNVWYEKDMRINIMKGKSFVPHNMHTEEDEAIIIN"
     gene            complement(9717..10814)
                     /locus_tag="PLJ75_04750"
     CDS             complement(9717..10814)
                     /locus_tag="PLJ75_04750"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPD04557.1"
                     /translation="MKHINYSTLLSYIKGELKHNDDDAIKLHVMQCQLCANNLKALGM
                     ITKTQRQYSPSPKILSNILDYYHQSLLTKSTFFDTFKAFIALHRRVFALATIIIAAGI
                     ALVLLSYRSNPITIPHVLYMADKSGHYIKSDTIKEGRRITIPETKWAMLVANNDIVFQ
                     LAGGVDMVIMRSHYSKNAGTKKFHYSLEKGAINIKTNHHHTSMHYEVKTPDANIQPLG
                     TEFYINVSPEGTHIYIVEGKVLLTNTATKVTIETEVGKLYTISQTQISSFDIEKYSLQ
                     WINDIDARFNNVAITDEDLGSSSILTSDVNNNETTTYQSQIDNTEKSNTQSSALNSQD
                     KEDKGNAKEMRNDMKHDEIKVMRKEAKQKHR"
     gene            complement(10872..11384)
                     /locus_tag="PLJ75_04755"
     CDS             complement(10872..11384)
                     /locus_tag="PLJ75_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD04558.1"
                     /translation="MIERETLINFYNLYSKEIFNYCYHLTGVYEAAEDVLQDCFINLI
                     EYSKKNVINSETVKSFLYKTAHNLAINYLKKNDKIFYLDDDNNYSSVDTIAETIANEE
                     MNNEILVAIQNLDVKTRSIFILKRDNNYTNEEIAKILNISERTVRRKLQQALSHIMVH
                     LKNKGFYDIL"
     assembly_gap    11627..11726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12781..13680
                     /gene="nadA"
                     /locus_tag="PLJ75_04760"
     CDS             12781..13680
                     /gene="nadA"
                     /locus_tag="PLJ75_04760"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPD04559.1"
                     /translation="MNLEESIARLKKEKKALIIAHNYQNPDVQDIADYTGDSLELSRI
                     AAKNDADIIIFCGVYFMAESACILSPQKKVILPDSSAGCPMADMADVDSILEMKKKYP
                     EAAIVTYINSTAAVKAMSDICVTSSNAVSVVNKLKEETILFVPDKNLAHYVQRFTSKK
                     IIPWNGFCPTHENFTKEDLMAIKKQFPGAHVIVHPECRSEVIDLADKTLSTGQMVNYI
                     RKIAHHQVIVGTEKGMLHKLKKISPEKEFILASRDFICPNMKKISLEKILHSLVNEEP
                     IVSVQEDIRVKALQSLQRMLELS"
     gene            13700..14782
                     /locus_tag="PLJ75_04765"
     CDS             13700..14782
                     /locus_tag="PLJ75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04560.1"
                     /translation="METLEFNEEVQKLRERTFNKEFRDLVAKVITKPASSTIDTPEKL
                     LEHFHQLYYIVLSKSFNDSVQFVDVESYIEDNSRHHGETVSSKLMSLGFAENLILQYD
                     FINTYFGILTNSVKGNFFSEFYLLPSDAIYNDVITQSEGVVISYKSIRDDQLYSKRLS
                     YVPDHDFYVWIFSTCHLVKKVISVLNITIPTVLELWCPIIMVKVYEKFSITSQLLNEI
                     YYATIIPIMTLLESYIIMNKSSLLLSSNYFHEVYKVFENYFYISKSENYTKYFILKSK
                     CENQLIYCLFRYLSQKYKDKKSFSTINFRPMIGLIIMFLTDEDEKLVSEAKNLVSILN
                     DYLEIAIIDKYMIKNAVQLYRYIIET"
     gene            14941..15771
                     /locus_tag="PLJ75_04770"
     CDS             14941..15771
                     /locus_tag="PLJ75_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04561.1"
                     /translation="MVFENNSGCVCRLLESYNVKGKKYQESDQIYLDDINLFARAGID
                     TIDIKYDEIIYSYFSTLYPAEFRIPYSSADFFAFDRKLETLERISTLTKRKRYLICVG
                     DIYAYDQHTGKRIIVFRHNDSIEYKQWNQVKRLVDHSKKLYYRNSENGIIIFVNLQPH
                     ADSSYIERFKKNADLVSAIVSRKKDCKIEISPDFLPTEDVYTINNPNDLLSFYQKSNA
                     RLIIIGETLNDDYRKALIKVRDYDKFARMMVVPNIDLRNVDHFLLQVKMVYNSDRWTE
                     "
     gene            15794..16714
                     /locus_tag="PLJ75_04775"
     CDS             15794..16714
                     /locus_tag="PLJ75_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04562.1"
                     /translation="MKKPKKKSLLKYFEPIESGKSLSENFGSLDLEGSFGYFALSAQN
                     KSLFLNRLSVDLLYEMMQKVGLLDHLKKIGFYNIKLEIDKDDAQIHYLKIYYDTIDPD
                     HLLIDLRVSESRFIPEKRFCEEYNAPMFMDMVVIEWLSAQNPKSDFNTSKPQLPGQSR
                     PGLGSLNYMMELMYIVGEQVVKDGFMDVPDHFHGAVMYAKKFKFFNPSHEAILRAILR
                     DIKGYSLYDMSWGMITGTIIDTITNKPQVYDPSEQIFPVSQRMKEYFNSKKYKEKYKK
                     VYESKKYYFDYDKMCKLREKVLKEKNPDEL"
     gene            complement(16756..17676)
                     /locus_tag="PLJ75_04780"
     CDS             complement(16756..17676)
                     /locus_tag="PLJ75_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD04563.1"
                     /translation="MYNFVDCSLNDYFDEAIKKYPNRIAYISQGYEMTYKEHGDIVYR
                     FAACLSDFGINKGNRVAIHLPNILQCVAAYYAIIKIGVIVVMHNPLYSPAELEFQFND
                     SGTKVAVTLDLFSNTIIDLRPKTKINQIVCVSFRDYLPSSVNPDTVLSVAPKASDNVY
                     KWTDLMKQYNPNPPAIKVTHEDIAQLQYTGGITGVSKGAILTHSNLTKQLQQIDAWDT
                     SIHRGDAKTMVGALPFFHVYGLSTVMNLNIYDGFKTILTARPTPESLIEIIQKYRPHI
                     ACLVPAMYIGMLNHPDFQKLDLSKVMLAKY"
     gene            17800..18543
                     /locus_tag="PLJ75_04785"
     CDS             17800..18543
                     /locus_tag="PLJ75_04785"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPD04564.1"
                     /translation="MNDLILTAAKIVKESISTIALTGAGISVESGIPDFRSAGGLWTR
                     YNPEEYAHIDGFRHNPRKIWKMIFEMRNLTKNAQPNPAHIALAQLEKMGLLNAVITQN
                     IDNLHQRAGSTKVVEFHGNAERLQCIYCGAEYPGEEFVLDDEPPHCKNCGEILKPSIV
                     FFGEMIPQMALMESQNLASDAKVVLVIGTSAVVYPASSIPHIAKANGAYIIEFNVEKT
                     VITPTITDIFIQGRVGETLPMFIEAITTL"
     gene            18553..>19562
                     /gene="mutL"
                     /locus_tag="PLJ75_04790"
     CDS             18553..>19562
                     /gene="mutL"
                     /locus_tag="PLJ75_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPD04565.1"
                     /translation="MELSPKIKNRICILPDSVKRKIAAGEVIDAPYSVVKELLENAID
                     AQASYIEIEIEESGLKRIAISDNGVGIVKDDLPLAVEEHATSKIRDINDIFAIHTMGF
                     RGEALASIAEVSQLTILSRTHDEPMGGKLVVTDNTTQMFDWAGATGTTVIVENLFYNT
                     PARKKFLKSIKGELNRIKDTIFCIAVAHPEIKFKIVIDKKMSFVFEPADSIQRIQQIY
                     GNEIASNLIDGSLQDLECTITGYISKPEYYRSNRSLQFLYVNKRPVQFPYFSFILQQV
                     YQTILQKGQYPVAFICMDVKPDLIDVNVHPAKREIRFFDNNYIHSMLYNFANKIISGG
                     IHT"
     assembly_gap    19563..19662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19663..20223
                     /locus_tag="PLJ75_04795"
     CDS             <19663..20223
                     /locus_tag="PLJ75_04795"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04566.1"
                     /translation="KDYAVLGVIFGEFIVIQKHDELYFIDFHAAHERKLYDYLLQVNT
                     TVAVQQLLIPVVIEFSPDMVMLVNSYRDVLLDYGLLLEQFDDNSVVVNAIPDFCTTSD
                     IEGLIKDVVDSLYENKNHIDNIKMTIIEKIACHAAKRAHDSITDADIIAITDYVFTCS
                     DYRCPHGRPFVYKLSKSELEGYFKRL"
     gene            20220..20756
                     /locus_tag="PLJ75_04800"
     CDS             20220..20756
                     /locus_tag="PLJ75_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HPD04567.1"
                     /translation="MKSKIILGISSSIAAYKSCDLARMLTRDGYDVFCVLTHNATHLV
                     TPLTLQTLTGNPVYTTSFALEWREMGHIELKDNAQLLLVAPATANIIGKFANGIADDL
                     LSTTFLSVNCPVVIAPAMNPNMWNHKAVQENITKLKKWGAIIVEPDTGLVACGDEGQG
                     RLASIESIYNIAINAIRK"
     gene            20740..21408
                     /locus_tag="PLJ75_04805"
     CDS             20740..21408
                     /locus_tag="PLJ75_04805"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HPD04568.1"
                     /translation="MRLENKKIIVSGGPTREWIDPVRYISNASSGKMGVAIADAAYNR
                     CINTVFIHGPVEHSIIANKPYHCIGVETTVDMLEAIQQELSPNTILIMAAAPADYAPV
                     HKSPVKIKKNNDTLTIALKRNPDILKTIATLRKENKQLRDVTLIGFAAETTNIMQYGK
                     TKLVEKELAMICINDVSSTDSGFGSDYNEVTIMLANEETMHLHKMTKKEVAENILQII
                     ETMV"
     gene            21470..22594
                     /locus_tag="PLJ75_04810"
     CDS             21470..22594
                     /locus_tag="PLJ75_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04569.1"
                     /translation="MKKLIVLFIVFIFPAYAFAQRQVDKKPVVHTVTIEFNGETIKGF
                     YCFIDARSNEEKSSNVENKALHGSVILFLQGHAQRADDAYKFTSQMALTSKSGVVIVV
                     VCDTPYGKDPQYCGDAGKEIIVMAMMQYALTLQGINVIDAEQMPEAITINGKTVSISQ
                     SGIPATLTVVGWSHGALLARRVANRYNCVKNLVQICPAGFNDWPDNSCAANCCVLSAF
                     SWESLRISTEMFKGHANDVFAASWGITKGIAGDSCRSCGSCLHGNFTVCKSLRAVKDI
                     TDVTLQANADNFPVTTLSSIVVIFGSNDSLFNPQKLLNYKESLNPQDVERFWLKYYPE
                     AVNNNARMNLFVLPGKHLAPVLYYKEYAFYTLKYINELKE"
     gene            complement(22595..23287)
                     /locus_tag="PLJ75_04815"
     CDS             complement(22595..23287)
                     /locus_tag="PLJ75_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04570.1"
                     /translation="MKRIMVQKLSYNYPDGTQALNDISFEVNGNQRVAVVGHNGSGKS
                     TLLCILSALLLPTSGMVKINDNNLTHKNKILIRKSTGILFSQVEYQFIMPDIINDVML
                     SINNGDDEEKYLQAMDIIKQVNLADVAFHHPLSLSSGQMKRAALASVLAKNPSVLLLD
                     EPLANLDKPSSDAVIDILLQLTMPIVFATHSKYLVDHVAQRIIVLDKGTIVMDDTAKN
                     NNVKKYLQTTLV"
     gene            complement(23300..23956)
                     /locus_tag="PLJ75_04820"
     CDS             complement(23300..23956)
                     /locus_tag="PLJ75_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04571.1"
                     /translation="MAFHYVKIIAVIVSLLFCSLLNAFSFLWTASSAVLAIIFLHYIP
                     GQHRTALIMIVPLVMTIVIMQLITGIIRDSIHYYIIALTAYKIVCISIIIIGARFFIG
                     HNGLKAFINLFPSTLRLFFLIFTRTLYTLMQHNRMIVFQLQSRINFKSKEKYYIPKYY
                     SVAFLYNQFYAMSHFRNGILSRAITSLPVMKLADTTTIANYITVGIILLLIIINGIFQ
                     "
     gene            complement(23956..24576)
                     /locus_tag="PLJ75_04825"
     CDS             complement(23956..24576)
                     /locus_tag="PLJ75_04825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04572.1"
                     /translation="MVNNNYDSKNIYFNNLSKSWDTTAGNNEARIKKLTEVLSWMHIK
                     KGDTVLDAGCGTGILFPIIEDYIGSTGRLIAIDASSGMIEVAQSKYKHCNNITYIAAP
                     LETIVMPLQSVNLIICFSVFPHIEDKLLALKKCHDLLADDGALYIFHLSDTKSLNEFH
                     SHLHAPVSKDFMPYKDELEQLLLDAGFVMKHYIDEPELNFIHAVLQ"
     gene            complement(24579..25106)
                     /locus_tag="PLJ75_04830"
     CDS             complement(24579..25106)
                     /locus_tag="PLJ75_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04573.1"
                     /translation="MHEEITITQIIIASITAACAVALPIIFHLVGLGSLFLPMFIPLA
                     IGSFFMTPFYACFTGAIAPITSSLLTGMPPLYPPIALLMVIELFFFCFIISVIIHRAP
                     EVNAYIVMIIAIIVDRFVLYMLYALVFPYFHISFALLGVYDLLKSIPGIILLLMTVPP
                     AVMGIKKLMKNYSWR"
     gene            25292..27007
                     /locus_tag="PLJ75_04835"
     CDS             25292..27007
                     /locus_tag="PLJ75_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPD04574.1"
                     /translation="MSKKFIPDWTEKAPEPYSYRSIFKWGDPHSFKHPNPKLYQELKT
                     VFNLDDSYFAKKHSEGRNTVAYKKPVRLTKKQLQDIADIAGYENISTTQFDRLKFATG
                     KTIEEAMKLRKGIVERVADCVVHPRSVDDVEKLIQYCHRHKIPVYVYGGGSSVNFGLR
                     TAKGGITLVLSTHMNNILHVNEKNQTVTVQAGIQGPALEEALNNAKKHFGTKYNYTCG
                     HFPQSFEYSSIGGWIVTLGSGQSSSYYGDAYDLVISMKWVTPAGTFTTRDYAATATGP
                     KINDIMKGSEGTFGVLVEVTWKIYRYMPQNRRRFGFIFKTFQDAINASREISQGEFGM
                     PAVFRVSDEEETHIGLKLYGIDGTILDKLMQLRGFKPMQRCLFISSAEGEKYFTKNIK
                     RQVKKICKKYGGMYITGLPTLMWEPGRYKDPYIREDLHDYGILIDTLETSVKWDNLHD
                     VHKAVRQFIKNRPQTVCMTHASHFYPQGTNLYFIYIMKTDDMNEYITFQDGIIDSIAK
                     SGGSLSHHHGVGRMLAPWMEEHIGKEQMAVLRAIKKHFDPNAIMNPGGQLGLDLKGKD
                     WRSIK"
     gene            complement(27183..28487)
                     /locus_tag="PLJ75_04840"
     CDS             complement(27183..28487)
                     /locus_tag="PLJ75_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HPD04575.1"
                     /translation="MKKIFFCILIVILSLAISLPFTYAAKLSDEDLAKKKEGWYPTGL
                     PLVNYDSDNGFGYGVRVYMYNNGSRDEENFAYAPYKMQLYGQFYQTTNGYQYHEVNLD
                     MPYIMGTKFRVITSVAYDKKINANFFGIGADTAKEKLTLYDGTNYHKFDKYEDYTDYA
                     DDNDNLQKYYNYKYTKPSFFLNVHRDLTQNLKILVGIEVKKVDIDAWDNHKFDHDRQG
                     PTLLKQWQPEGYDGGWSNFVRAGIYYDTRDYEPDPKRGYLIDYAYEISDNVLGSDYDF
                     MRNTVQLQGYIPLLSSLTLALRAAYTDTNSDTPFFEMGYFGFSLNRRTGLGNNRTLRG
                     YREQRFVGPTMTVGNAELRWKFAETNAWGQNFQFKLTGFYDVGNVYDEAGDPFSDPRF
                     GDYHQGYGGGLVIAWNMATIVHFYYGTSEEDTSISVDFSHTF"
     gene            complement(28622..29518)
                     /locus_tag="PLJ75_04845"
     CDS             complement(28622..29518)
                     /locus_tag="PLJ75_04845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04576.1"
                     /translation="MKTFTFDSPLTSCPLCNSNAIYFLYAIVTCNPAFTVYKCNSCKF
                     IFMNPPLSHQSEHTLYDEGYYTGTSDYSYYDERITERYSQYVWNARLHTIHNYVPGGN
                     FLDVGCSFGGFLHAASQLYITYGIEPSAYSGKYAQQRFGSDVIHIGTLFDHPFKHNFF
                     SVITLIEVLEHMRQPYEALTECYNLCKQDGLLVIQTANMDGLQAAIQKERYGYFLPGH
                     FSYFTKDNLTHTLKKIGFTKIKVFQPVDFGLLPKLMKSRMNFNSIYAYKSWLRIALYH
                     YASKIHAGNCALTSSMVVYAFK"
     gene            complement(29515..>29967)
                     /locus_tag="PLJ75_04850"
     CDS             complement(29515..>29967)
                     /locus_tag="PLJ75_04850"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04577.1"
                     /translation="VIGVDISYNALKQSQSLLDNIFIQCSINKLPFGDSSFDAVIAWG
                     SLHYAKKELLPCMLAEIKRVLSSEGIVYGTLRSEHDTYIARGKHLGNNMWETSLPDIQ
                     GAVISLYSLHEVKKYLSIFKEFHIGLMERTVLDDLNKKLSHWYFYATV"
BASE COUNT         9935 a         4849 c         5420 g         9463 t
ORIGIN      
        1 ctgatgttgg tgtaccgata gaccatcatg agtactttct gcagctgggg gacagtttta
       61 gcgaacgggt gagacgtttt ttgcagtatg aggaggcgta ccgtaagcgc tactccattg
      121 cacagtgggt ataaaataaa aaaggggtca gagccttata cctcaaccgt aaaattaatc
      181 tttatttata cacagcttcg tgatacaatt aatcacatga taattgtagg tataacaggt
      241 gctagcgggg cactattagg catacgctgt attgaagagc tggtatatca taataaacaa
      301 attgctgtta tagtatccaa gagcgcatgg cctatcatac accatgagat gcaaacagat
      361 tgtacctcga ttataagcat tgttaaacaa cgaactatgc aaccggaatt gttaagtaat
      421 attatagagt atagtcctga tgattttttt gcacctatag caagtggcag taatcaattt
      481 gaagctacaa tagttatacc ctgctcaatg aaaacagtgg ctgctatcgc tcatggatat
      541 gcagataatt taatccatag ggtatgtgat attgcactga aggaaaaacg gcagcttgtt
      601 atagtgcccc gtgaaacccc tttgagtgca ctacaccttt ataatctcta tacattatca
      661 atgtatgggg cttcagtggt aatgcccata ccagcttttt ataattttcc tgaaaccgtc
      721 gatgatgtag taaattttat cataggcagg gtatttgacc agttacatat tcagcataac
      781 ctctataggc gatggcatta tgaagaacat acacgatgaa aaacttgccc ttatttataa
      841 taaagttatt gcgggagtac cagtcagtaa cgatgacgca tacacaatgc taaccaccaa
      901 tgatatcatg ggacttggct tcattgctaa tacagtaaaa aaggaaatgc atggcaatat
      961 catctactat ggtgttaata tgaatctcaa ttacaccaat atatgtttgc tgcgctgtcc
     1021 tttatgtgct ttctcacgcg ataaggatga ccacgatgcc tattgtttat cacatgatga
     1081 gatagttcat aaaataaaaa atgctgctga cattgatgag gtgcacatcg ttggagggat
     1141 acatcccgat ttgccttttt catactatga agagatgatt gcattgataa aatctaccag
     1201 gcctgatatt cacattgttg cttttactgc tgttgaatat gactatatgg caaagaaatt
     1261 taatattccg cttgatgagc tatttcagcg attaaaaaaa gctggcattg attccatacc
     1321 agggggaggt gcagaaatac ttgccaatca tatccgaaca actatcgccc caaaaaaaat
     1381 aagtggacaa cggtggctgg aggttatggc gacagcacat agaaatggaa tgaaaagcaa
     1441 tgcaaccatg ctttttaatc atatcgaaga tactgctgat attgttgatc atatgtgcaa
     1501 aattcgtgat ttgcagaata ctaccggtgg attcaaaacc tttgtgcccc tcgtattcca
     1561 tgaagaaaat acaggggtca gagccagacg gaaagatcca accggctatg acatcatccg
     1621 tatttatgct actgcgcgca tatatcttca taatattccc cacataaagg cgctgtggat
     1681 gtatgtaggt gaaaagcttg cacaggtgtt actgcaatgc ggtgtggatg atattggtgc
     1741 tacatattat tatgaaaagg tggtccacag tgcaggtgca aaaacgcctg catgggggtc
     1801 agagcctttt ttgcagcact taatttatga cgctggtttt aaacctgtgc gggttaacgc
     1861 cagctatgaa gcaataaaaa aatagcaacg aggtaccatg aaattagggt atattaatta
     1921 tttaaactgt tatccttttt actttaacat gtttgaaaaa aaaccacttc ctgatgttga
     1981 ggttattccg gcatatccaa gccagcttaa cgcaatgata aaagaaggga agcttgcaat
     2041 gagtcctata tctgctgcag cgtatgcaga aggctataac gaccttgtac tgttacctga
     2101 cttctgttta agttcgattg ggtatgtgcg ctcagtcata ctggtgagca aaaaacctat
     2161 tgaaaattta aaccacagca ccgttgggtt atcgagtgca tcgcaaacat cggtagtgct
     2221 gttaaaaatt ttattacaaa aatattacgg gctggtccca aactatattc catctgatcc
     2281 ttatgcaaca ctgcaccatg ttgacgccaa actggttatt ggcaacgagg caatgatgca
     2341 cgaaaccgaa ccgcttccct atgtatatga ccttggcgat ttatggctta acaaaacagg
     2401 atatcctgta gtgtttgctg tatttgcagt ccgcaatgat gcactggata gcaatgaaaa
     2461 aactatcaat gcagtttgcc aatcgtacca cacatcgctt ttacaactta aaaacaatgc
     2521 acccgaatta attgcatgtg ccagtcttcg ctatcctgat atagcgtatg atatcgcaac
     2581 atactataca ctgcttaaat ttgaatttgc aaacaagctc aaagaagcgt tgatgtttta
     2641 ttttaccagt gctcatgaat tgggcttgct gccatatatt ccttcgttac aattttatgg
     2701 gcgatagtta ctgtatatga ataccgttga ctcaataatc aatgccgcat ataacaacaa
     2761 acgcctggag cctcatgaag ccgttttact ttttgattgc gatgttgcga ccttaggagt
     2821 tttagccaat tatcgcagag aacaaatagt ctccggcaat gaggttggct ttgttataga
     2881 ccggattata aattattcta atcgctgtca ggcaatgtgt aaattttgtg cattccatgc
     2941 acaagcaggg aagatacagt catatgatct gacggttgat gatattttgc aaaaaattga
     3001 tgagctggta catataggtg gcactcaggt aatgctgcag ggaggattgc atcctgacta
     3061 tacattacag gattatgttg ccatgataaa aactatcaaa cagcattatc ctgatattta
     3121 tcttcattca ttttcaccat cagagatagt ccatattgca aagcgcagcg gcagtacaat
     3181 tgatgctatt atagatactt tacaacaggc agggttggat tcaatccctg gcgcatcaga
     3241 tcttctggtt gacgatatcc gtaaaaaggt aagtccgcgc aaagctacag tagcacagtg
     3301 gtgcgaggta gtccgttcaa taaaaaaaca tggtatgcac acaacagcta ccatgaccta
     3361 tgggatgggt gaaacaaagc ttcaacggat agagcatctt gatgttatac ggaaaatgca
     3421 ggatgaaaca ggttgtatta tggcatttat tccatggtca tttgcaccaa agggaacaaa
     3481 tatggacact ataatccctg caacagggat tgattatcta aaaactgttg ctatttcacg
     3541 aatatttctg gacaatgtac cttatttaca tgcgggatgg ctaactgaag ggatggctct
     3601 ggcacagata gcgctggcaa tgggtgcaaa tgatatgggt ggcatattga tggaagagaa
     3661 ggttgtaaag gctaccggtg taacaaccac aacaaatata ccgcagttgt gtgatgttat
     3721 cattaatgca ggcaaaattc cggttcacag agattccatg tatactgtac tgcggagaat
     3781 ggtatgagat acatcattga taacaaaaat cccaacactc gcaaacaaca aatcaggacc
     3841 atgtttgatt caattgttga aggttacgat agggcaaaca ggattttatc attagggatt
     3901 gatannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngaagta ttcagggtgt
     4021 tacaacccgg agggatgttt gttgcattgg agtttacccg tccacgcaat cctattgtgc
     4081 gattaatcta ttttttatat ctctatacat tcctgcctat gattggtgga attatcgcag
     4141 ggaacataaa agcatatcac tatctatcaa agaccataca tacatttatt gatccgccat
     4201 cattactggc aattcaggaa aaacatggtt ttgttgaaat aaagtgtaat tccatgacac
     4261 tgagtattgt taccgcatat tcaagtataa aaccaaagtc cctatcatcg cctttaagct
     4321 tacagcattt aaacaactta cagcacaaca gggtgataat acattagata gaattgatgt
     4381 ttcatcagtg tttttttatc agtaaccatt caattagtag ataaagtctg ggaatcagga
     4441 tcaaaatgtt ttgaatatca taccaatttc aagttgatag gtataaaggt aatcacgcca
     4501 gcttgtgtct tcaatgacat tggtaaagta aaattggcca tgtggtgcta tataaaaata
     4561 ggtaaaacgg ttaaatgcaa ataccaatcg caacattaat cctgccccaa actgattata
     4621 attgatatat ctaatcccat cggtatgttg attgttgctg ctcatggtat tatgaaaaat
     4681 gtgcatgtaa ctcaatccat ataagatatc aatacgaatt gtattgccaa atgatgatag
     4741 caaatataat ggtattatta tagaatgatt ttttgatttt aatttttcat cagtactatc
     4801 ctgaaatacc aattcacttt caaggtatga atagtttata ccagcatgga tgccaaagct
     4861 attcgtaaac atgttttcaa aaaataaccc agcaccagcc ataatacctt gattccatgt
     4921 aatatccttc ccatcatcct tgccactttt atgccataca tcatcagctc caccggataa
     4981 agaaaatcca aaggaataat ttgaataatt attctgtgct atagctaaag tagatgatat
     5041 aaaaacaact ataacacaac taactttcag caaatttttt tgaaaaatca tgttttagtt
     5101 gcctattatg ggttttatac tattttggca taatacaggt gccattaaac tttgcgccat
     5161 tttcaacata caaatcaggc gttacgatgt cacctgacat attgctgtgt tcaaaaattt
     5221 caataagttt agaagctttg attttaccct gacatttacc acttattgaa acagtagcag
     5281 cggtaatttc tgcacttacc ctggcatcat ctcctataat gagcgtgccg tcagtttgaa
     5341 cttttccttc taatgaacct ttaatcttta atgatgattt aaacttcaat gtaccccgga
     5401 atgaaatatc gcttgcaatt actgtagcta tcttgttttc atcctctata atttcttttt
     5461 gttctaccat attgtatcct ccgaaaatag aattatcctc tcaatgttat tccaggcgtt
     5521 acccggaatc tgctttcgcc gccttcgtca ttccggactt gttccggaat tcaaatgact
     5581 gtcgccaaat atatgtatcc caatttctca ggcacaatgc tatacattgt aaactcatta
     5641 aaattaaaat attttatcat tactgctgta atgtcaattc aatttatcag aaatagcata
     5701 tttttaaaaa aaaagaaaaa aaatccttgc ataaatccat catagattat tatagaaaga
     5761 aagtgtaata tatgctataa tgtattgtat agtaaatctg ctctatagag aataacaatt
     5821 ttatcaaaaa ctattgaagc gataataatg aaggagaaat attgtatgga ggaaatttta
     5881 caaaaagcaa atgaattagg gctaatgatt aagggcagtg atatttttaa gcggtatgaa
     5941 gaattggtaa aaaaaattga agctgatagt aatgcacgca cattgttaga tgaatatata
     6001 aaaatgagtg aagagatata taagaaagaa tcatcaggtg gtgtagttga agttgaggaa
     6061 aaacaaaagt tgcaggactt aagccagaag gtatcagaaa atcagcttat taaagagttt
     6121 attgcaactc agagctatta tgttaacatg atgatgcaga ttcagaatac cattagcaat
     6181 ccaaaaggtg atccaatagg gccatctaaa ataataaaac cgggtgctgg aactaccggg
     6241 aagatagtta ctggtaactt ttaacagcag agacaaccat atctgtcgtc tctgcgtggt
     6301 tgttattata ccagtttctc tatagcttct tgtatccgtt ttaatccttc gatgatactt
     6361 ttatcagatg ttgcaaacga taaccttaca ttattatctg aaccaaatgc aacgccaggt
     6421 acaactgcaa ctctggcttc ttcaagtaaa taagaagata attttgacga taacgaatca
     6481 cctttatagt tatctataat gtgtttaaag ccattacatt gatatatctt ctttacattg
     6541 gggaatacat aaaatgctcc atccggatta tggcattcca caccgttaat gtcattaaga
     6601 cccttgacaa tcatctcttt tcttctggta aatgcttgta acatttcatt aataaatgat
     6661 tggtcgccca taatagcttc tactgctgcc cattgtgcaa tggatgttgg attggaaacg
     6721 gattggcttt gtaccatttc aacctgttta atgatggttt catctcctgc catgtaacca
     6781 atccgccagc ctgtcatgga ataggttttt gaaacgccat tgagcaccat agttttggat
     6841 tttaattcag gtattgcatt gagaatatta taaaacttgt taccattgta tacaatgctt
     6901 tcatagatat catcagttat gatacatata tcataatttt taagcaattc acctaatgct
     6961 ttcaattcat ctaatgtata catggagcca gtaggatttg aaggagaatt gataacaacc
     7021 gcctttgttt ttttgttgat atgttttttt aacatttcgg gggtaatttt aaaatttgtt
     7081 agttcatcgg tatgtataac aacgggagtt ccatcagcta acaatacaat gtcgggatat
     7141 gaaacccagt agggtgcggg tataatcacc tcatcaccac tattgagcaa cacctgcatg
     7201 aggttataaa atgaatgctt tccgccgcaa ttaacagtta cctcagataa tttatattct
     7261 aagttattat cccgtttaaa tttttgaact atggcttttt ttaattcagg aatacctccg
     7321 gaaggtgtgt atttggtttt tccatccact aatgctttta ttgctgcatc tttaattgac
     7381 tggggagtat caaaatcagg ttcgccggaa ccaaatccaa taacatcaac tccctgtgct
     7441 tttaaagaat tagcaagcgc agttaccgct aaagtagctg aaggtttaat cttttttaat
     7501 ctatgagcta actccataac ttctcctttc atggtatata gaatatcaca atatttgata
     7561 aaaaaaggga gtagaaaaaa cgtagtatgt gggagatctg atttatatca aaaagaacaa
     7621 caaagctata atcttatttt gtcaagaaat ttatgcatta aaaacaatat aaatatttaa
     7681 aaattaccaa tttaagtaaa ttcaaaaaaa cttataataa atagtcattg acatatatag
     7741 tatatttgtt tacactttga cctgagatag cacggtgatt atgttcataa aatatagttg
     7801 catattgttt atatgacatc tatagtaata gtgctgtgat tgcaaaacta tttagaggta
     7861 attgggtgca attatcaata cgagggggta aatgatatga aaaggataac aatgataatc
     7921 ataattacag tactgtcaac tgtgttatta gcaaaaaatg cattctttaa aggtcgtgta
     7981 atatttacca ataaagatta tattgaagtt aagtatggca aaactgaaaa acagttttat
     8041 gtcaatgata aaagtgtatt caaaaaaggt ggaacaaatg taacgtttgc ggatatcaaa
     8101 ccatgtcaga tagtaaaagt agtttatgct gtcgctgata aaacaatgta tataacgaca
     8161 tgtgaaataa tcaaagaaag cgattgcgaa tgattgtata atctcatagg ctattttttt
     8221 tggcgatagt gacttcataa caatctattt tgcattgata agtgcctgcc agtaatcaat
     8281 tttacagtat atgccgcagg gatataatta tcgctcaaaa tatttgaatt gcatgacatg
     8341 tcagaatgtt gatatgcaca aaaaaggtta tcgttttgtc aatgtgcaag cggaatgcag
     8401 atgatgtatg ctcactgttg ccatgatatg ctgatattgt ttcacaggag ctatcgcccc
     8461 catgtaataa aaatagagga taagcatggt gtttgtagta ttgccacaac aatccaatga
     8521 caagacatga aataattaca ggtgcatgct attcgcaaga acatgatacc atttgacatt
     8581 atccaaaata atgtaaaatt tttatagttt aaaaataaat ttgcaaccaa aatacccata
     8641 ctgctgtctt tactatacac tttggttcta ctacttataa agaatggtat aacagtaaac
     8701 cagtaaatta ttacaaataa tgttatatat aaatataatt aaggagagat cggtgaagag
     8761 cttaaaagaa aatcttgttg gtgctttaac agttatgttg attgtaataa acacattatt
     8821 ctgggcttgc tttttatttt cagttgctgt gctaaaaatc atcccgttga gatttacaaa
     8881 aaagtatatt aatacaatat tggataccat agcccgttta tggatttatt gtaacaacgc
     8941 tggtttaaaa attacaagaa aaatagtatg ggatgttgaa ggtgataata atctcacaac
     9001 aaatagctgg tatttagtaa tagctaatca tcagtcatgg agcgatattg ttgtgttaca
     9061 aaaaatatta accggtaaaa ttccattttt aaaattcttt ttaaaaaaag agcttatcgg
     9121 agttccagtt ttaggacttg catggtgggc tttagatttt ccgtttatga gacgttattc
     9181 gcaatcattt ttattaaaat atcccaatct gaaaggcaaa gatattgaga taacaaaaaa
     9241 agcctgccaa aagtttaagg atatacctgt atcggttatg aattttgttg agggaacacg
     9301 ttttacaaaa gagaaacata caaagcaggg atcaccatat agtaaccttt taaaacctaa
     9361 atcaggaggc attggttttg ttttttcaac aatgggtgat tacattacat caattcttga
     9421 tgttactatc gtatatcctg aaggtaacaa atcattctgg gattttttat gtggtaggat
     9481 tactatgata aaagttcaga tagatgaaat tccagtgaca gaggcattga gaggtgatta
     9541 tatagatgat ccggttttta agagaaaatt tcagcaatgg ctcaataatg tatggtatga
     9601 aaaggacatg agaattaata ttatgaaagg taaatcattt gtgccacaca atatgcatac
     9661 cgaggaagat gaagcgatca ttataaatta accatacaga ttaaatatag ctaatattat
     9721 ctgtgttttt gttttgcttc ttttcgcata acttttattt cgtcatgttt catgtcattt
     9781 cgcatttctt ttgcattacc cttatcttct ttatcttgac tgtttaatgc tgaagattgg
     9841 gtgtttgatt tctcagtatt atcaatttgt gattgatacg ttgtagtttc attattattt
     9901 acatcagatg ttaatattga tgatgaacct aaatcttcat ctgttatggc aacgttatta
     9961 aaccgggcat caatatcatt gatccattgt aatgagtatt tttcaatatc aaatgaacta
    10021 atctgagttt gcgatatggt gtataattta ccaacttcgg tttcaattgt tacttttgtt
    10081 gcagtattgg ttaatagtac tttaccttca acaatataaa tatgtgtccc ttcaggcgaa
    10141 acattaatat aaaattcagt acccaatggc tgaatattgg catcaggagt tttcacttca
    10201 taatgcattg atgtatggtg atgattagtt tttatgttta tagcaccttt ttcaagtgaa
    10261 taatgaaatt ttttagtacc agcattttta ctataatgcg atctcattat gaccatgtca
    10321 actccaccag ctaactgaaa aacaatatca ttgttagcaa caagcatagc ccatttagtt
    10381 tcaggtatgg taattctacg tccttctttg attgtatcag attttatata atgccctgat
    10441 ttatctgcca tatataatac atgtggtata gtaatgggat ttgatcgata acttaacaat
    10501 acaagtgcta tccctgcagc tataataatg gttgccagtg caaatactct tctatgcaag
    10561 gcaatgaacg ctttgaatgt atcaaaaaag gtagattttg ttaataatga ctggtgataa
    10621 tagtccagaa tatttgataa tattttaggt gaaggggaat attgcctttg agtttttgta
    10681 atcataccta atgctttgag gttgttagca catagctgac attgcataac atgaagcttt
    10741 attgcatcgt catcgttatg cttcaattca ccttttatgt atgacagcaa tgtactataa
    10801 ttgatatgct tcatggcatt aataacccct taattataat acaattaaaa taagcaaaat
    10861 ggtcatatga ttcataaaat atcataaaat cctttatttt taagatgtac catgatatgc
    10921 gataatgctt gttgtaactt tctgcgaact gtacgttcag agatattgag tatttttgca
    10981 atttcttcgt ttgtgtaatt attgtcacgt tttaagataa atattgatcg agtttttaca
    11041 tctaaatttt gtattgctac taatatttcg ttattcattt cttcattggc gatagtttct
    11101 gcaattgtgt ctactgatga ataattgtta tcatcgtcta aataaaaaat tttgtcgttt
    11161 ttctttagat agttgatggc taaattatga gcagttttat ataaaaatga ttttactgtt
    11221 tcgctgttga taacgttttt ctttgaatat tcaattaaat tgataaagca atcctgtaaa
    11281 acatcttctg cagcttcata aacacctgtt aaatggtaac agtaattaaa aatctctttt
    11341 gaatatagat tataaaaatt tattaatgtt tcacgttcta tcataaaata taaaaaaaaa
    11401 tgtccgttat ttttgtatca gattgtaata tatatcaaat agttagtaag tcaatatttt
    11461 catcaactaa aattttataa caaattcata atcaggagga ttttatgaaa aaggttttag
    11521 ctacattagt atcattgttg tttgcagctt cagtttttgc acagacaggt gtgcagacaa
    11581 aggaagctaa aaagaatgtt gggcaagaaa aaaagataga agttcannnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnngaag taaagaaaga agtaaagaaa gaagaaaaag
    11761 cacagaagca agaacagaag aaagaagcta aagaacagaa gaaagaagct aaagaacaga
    11821 agaaagaagc taaagaacag aagaaagaag aaaaaactgc aaagtaataa caacaagatg
    11881 aattgtatgg gaaaaaagcc atgctttata agtatggctt tttttattgt gtaaaaaaat
    11941 aattaatcaa tgattagcag cacttttttg taacctcaga atcgactgtt tttgagttta
    12001 aattaaaatg acctttaata tctaactatg gaatagaaaa ctactttttt tgaatttaat
    12061 ttttatcaaa tttttgccaa aaaacttgcg tattaccttc cattatttgc tttaattgtc
    12121 catcaatgta ttaaaatctc aatcatcaaa aggactgcta accttatata ataaaattac
    12181 ttctttactg cacaacatct accctgtatt ttgcagtaca ttttaacatt aatgatacat
    12241 ctattattat gaatgtttta cagcattatc agcaaaaaaa ttgccattct caagcttatt
    12301 gtatgttctg gttcatattt atcgggcact tttaagctct tattgtaata ttcttgtaat
    12361 gtctcacgtg ggcttctata tcttaatcgt tttatcatct ttgtatggtc gtagcgctca
    12421 atagattttt catttacatc tctggtgggg ctatatacta tataaattat atggcgaacc
    12481 atatcactgt atagcttact actcgtattg ttgttgcgat actgttatct tcctcagcag
    12541 gtttttaata attgcgctat tggcatatac actatctgct ataagttcat gtgttttcat
    12601 caacaactat cgcctcgaaa tgttctttaa aaaatactat tactatgctg ccgtctatat
    12661 tattttcaat attcattaaa ctaccttttt caaaaggcaa taatataatt gcataaaata
    12721 gacaatgtgt tttaatgttc aaaattgcca ttataaatta cattcaaaag gaatataaat
    12781 atgaaccttg aagaatcgat agcccgatta aaaaaggaaa agaaagcact aatcatagcg
    12841 cataattatc aaaatcctga tgttcaggat attgctgatt atactggtga ttcactggag
    12901 ctatcgcgca tagcagcaaa aaatgatgcc gatatcatta tattctgtgg cgtatatttc
    12961 atggccgagt ctgcctgcat actgtcacca caaaaaaaag ttattttacc tgatagcagt
    13021 gcaggttgtc caatggctga tatggcagat gttgattcta ttcttgaaat gaaaaaaaaa
    13081 tatcctgagg ctgctatcgt tacatacata aattctacag ctgctgttaa agctatgagt
    13141 gatatatgtg ttacatcatc gaatgctgtt tcagttgtaa acaaacttaa ggaagaaaca
    13201 atactatttg tgcctgataa aaatctggct cattatgtac agcgctttac cagtaaaaag
    13261 attattccat ggaatggttt ttgccctaca catgaaaatt ttacaaaaga agatttgatg
    13321 gctattaaaa aacaattccc gggtgcgcat gtcatagtcc accctgaatg cagatcggag
    13381 gtgattgatt tagctgataa aactttgtca accggacaga tggttaatta tatcaggaaa
    13441 atcgctcatc atcaggtaat tgttggcacc gaaaaaggca tgttgcataa attaaaaaaa
    13501 atatcgccag aaaaggaatt tattcttgca tcaagagatt ttatatgtcc aaatatgaaa
    13561 aagatttcat tagaaaaaat tttacattct ttagttaatg aagaaccgat agtatcagtg
    13621 caggaagata tcagggtaaa agctttacaa tcgctgcaac gtatgttaga attatcctga
    13681 aatgtggaag aggcgtgctt tggaaacatt agaatttaat gaagaagtac aaaaattacg
    13741 agaaagaaca tttaacaaag aatttagaga tctggtagct aaagttatta caaaaccagc
    13801 atcaagtaca attgatacac ctgaaaagtt attggaacat tttcaccaat tatattacat
    13861 tgttttaagt aaatctttta acgattctgt acagtttgtt gatgtagaat catatattga
    13921 agataattcc cggcatcatg gtgagacagt gtcttcaaaa ttaatgtccc tgggttttgc
    13981 tgaaaatctt attctgcagt acgattttat aaacacttat tttggtattt taacaaattc
    14041 tgttaaggga aacttttttt cagaatttta tttattacct tcagatgcta tttataatga
    14101 tgtaatcaca caatctgaag gcgttgtaat atcatataaa tctataagag atgatcagtt
    14161 atattcaaaa agacttagct atgtaccaga tcatgatttt tatgtatgga ttttttcaac
    14221 atgccatctg gttaaaaagg ttatatcggt tttaaatatt accatcccca ctgtacttga
    14281 attgtggtgt cctataatta tggttaaggt ttatgaaaag tttagcataa catcccaatt
    14341 attgaatgaa atttattatg caactattat tccaataatg actttgcttg aatcttatat
    14401 aattatgaat aaaagttcat tgcttttatc atcaaattat tttcatgaag tgtataaagt
    14461 ttttgaaaat tatttttata tatcaaaatc agaaaactat acaaaatatt ttatattgaa
    14521 gtcaaaatgt gaaaatcagt taatttattg tcttttcaga tatttatcac aaaaatataa
    14581 agataaaaaa tctttttcaa caattaattt ccgccctatg attggtttaa taattatgtt
    14641 tttaactgat gaagatgaaa aacttgtgag tgaagctaaa aatcttgttt caatattaaa
    14701 tgattacttg gagattgcaa taatagataa atatatgatc aaaaatgcag ttcaattata
    14761 tagatatatt attgaaactt aaacaaatta attattatat tgatatttca aaatgtactt
    14821 gaataaaaaa ccatattgtt tttatatttc ttttaatagt atataatgtg tgccgatgat
    14881 ataataaata aaagtaaaat tttttattat ataatgttgg taaaaattga tcaattaaaa
    14941 atggtttttg aaaataattc aggatgcgtt tgtagactgc tggaaagcta taacgtaaaa
    15001 ggaaaaaaat atcaagaatc agatcaaatt tatcttgatg atattaatct ttttgcccgg
    15061 gctgggattg ataccattga tattaaatat gatgaaataa tttacagcta tttttctaca
    15121 ctatatccag cagagtttcg catcccttat tcttcagctg atttttttgc ttttgatagg
    15181 aaattagaaa cattagaacg aataagtacg cttacaaaac gtaaacgata tttgatatgt
    15241 gttggtgata tctatgcata tgatcaacat acaggaaaaa gaattatagt gtttcggcat
    15301 aatgatagca ttgaatacaa acaatggaat caggtaaaaa gactggttga tcatagtaaa
    15361 aaattgtatt atagaaactc agagaatggc ataattattt ttgttaattt acaacctcat
    15421 gcagattcaa gctatattga acgttttaaa aaaaatgcgg atctggttag tgcgatagta
    15481 tccagaaaaa aagattgtaa aattgagata tcaccagatt ttttacctac agaagatgtt
    15541 tataccatta ataatccaaa tgatcttctg tcattttatc aaaagtcgaa tgcacgtctt
    15601 attattattg gtgaaacttt aaatgatgat tatagaaaag cattgataaa ggttagggat
    15661 tatgataaat ttgccaggat gatggttgtt cccaatattg atctgagaaa tgttgatcat
    15721 tttttattac aggtaaaaat ggtttataat tctgaccgat ggactgaata attcagaaaa
    15781 gaaggaataa tttgtgaaaa aacctaaaaa aaaatcacta ttaaaatatt ttgaacctat
    15841 tgaatctgga aaatcattat ccgaaaattt tggatcgctt gatttagaag gaagttttgg
    15901 ctattttgca ctaagtgctc agaataaaag tttgttttta aatagattat ctgttgatct
    15961 tctctatgaa atgatgcaaa aagttggatt gctggatcac ctgaaaaaaa ttgggtttta
    16021 caacattaag cttgaaattg ataaagatga tgctcaaata cactatttga aaatatatta
    16081 tgatacaata gatccagatc atttgctcat agatttaagg gtttctgaat caagatttat
    16141 tcctgaaaaa agattttgtg aagagtataa cgcacctatg tttatggata tggtggtaat
    16201 tgaatggcta tcagcacaaa atccaaaatc agattttaat acttccaaac ctcaacttcc
    16261 tggacaatcc aggccgggtt tgggtagttt aaactatatg atggaattga tgtatattgt
    16321 tggtgaacag gtggtaaaag atgggtttat ggatgtgccg gatcattttc atggtgcagt
    16381 gatgtatgca aaaaaattta aattctttaa tccaagtcat gaagcaattt tacgagctat
    16441 actcagagat ataaaaggtt attcattata tgatatgtca tgggggatga ttacaggaac
    16501 tataatagat acaattacca ataaacctca ggtatatgat ccatcagaac agatattccc
    16561 tgtttcgcaa cgtatgaaag aatactttaa ttcaaaaaaa tataaagaaa agtacaaaaa
    16621 ggtatatgaa tcaaaaaaat attattttga ttacgataaa atgtgtaaac tcagagaaaa
    16681 agtacttaaa gaaaagaacc ctgatgaact ataattaatt cattttcttc atttcttctt
    16741 ctcgcaaaac ttttctcaat attttgccaa cattactttt gataggtcaa gtttttgaaa
    16801 atcaggatga ttgagcatgc ctatatacat agctggtact aaacaagcaa tatgaggtct
    16861 atatttctgg atgatttcaa taagtgattc aggagttggt cgtgctgtta atatggtttt
    16921 aaatccatca taaatattca aattcatgac agtagataaa ccgtatacat ggaaaaatgg
    16981 caaagcgcct accattgttt ttgcatcacc tctatgaata gatgtatccc atgcatctat
    17041 ctgttgtaat tgttttgtta aattactatg agtgagaata gcaccttttg aaacacccgt
    17101 tatcccacca gtatactgga gttgagcaat atcttcgtgt gtaactttta ttgcaggcgg
    17161 attaggatta tactgtttca ttaaatcagt ccatttgtaa acattatcag aagcttttgg
    17221 tgcaacggat aatacagtat cagggttaac tgaggaaggc agatagtcac ggaatgaaac
    17281 acaaacaatt tgattaatct ttgtttttgg tcttaaatca ataatagtat tactaaaaag
    17341 atcgagcgta acagcaactt tagtgccaga atcattaaac tggaattcaa gctctgcggg
    17401 tgaatagaga ggattatgca taaccacaat cacgccaatt tttataatag cataatatgc
    17461 agcaacacat tgaagaatat ttgggagatg gatggctact ctatttcctt tattaattcc
    17521 aaaatcagat aaacatgcag caaaacgata gacaatatct ccatgttctt tatatgtcat
    17581 ttcatatccc tgcgatatgt aggcaatcct attgggatat ttttttatag cttcatcaaa
    17641 atagtcattt agtgaacaat ctacaaaatt atacaatata aagatgctta ttatatacaa
    17701 aattgaaaaa attacttgcc aatatactat gagtgtattt tctttattat catgattata
    17761 cattgaggac tataattatt ataaggtaat accattatta tgaatgattt aattttaaca
    17821 gcggcaaaaa ttgtaaaaga atctatatca accattgcgt taaccggtgc cggaatttct
    17881 gttgaaagtg gcataccgga tttcaggagt gcaggtggat tatggacccg ctataatccc
    17941 gaagaatatg cccacatcga tggattcagg cataatcctc gcaagatatg gaagatgata
    18001 tttgagatgc gaaatttaac aaaaaatgca cagcccaacc ctgctcacat tgcacttgca
    18061 caattggaaa agatgggatt attaaacgcg gttataactc aaaatattga taacctgcac
    18121 cagcgagctg gaagtacaaa ggttgttgag ttccatggca atgctgaacg tttacaatgt
    18181 atttattgtg gtgctgagta tcctggcgaa gaatttgttc ttgatgatga accgcctcat
    18241 tgtaaaaatt gtggtgaaat tttaaaacca agcatcgttt tttttggcga gatgattccc
    18301 cagatggcgt tgatggaatc acaaaattta gccagtgatg caaaggttgt tcttgtcatt
    18361 ggaacctctg cggtggttta tcctgcaagc agtatcccgc atatagccaa agctaatggc
    18421 gcatatatca ttgaattcaa tgtagaaaaa acagtcatca caccaaccat tactgatatc
    18481 tttattcagg gcagagtggg agaaacatta cccatgttta tagaagccat tacgacgctg
    18541 taggctcgat atatggagct atcgccaaaa ataaaaaata gaatttgcat attacctgat
    18601 tctgttaaaa gaaaaattgc tgccggcgag gtaattgacg caccgtattc agtggttaag
    18661 gaattgctgg aaaatgctat agacgcccaa gcctcatata ttgaaattga aattgaggaa
    18721 agtggattaa aaagaatagc aatttcagat aatggcgtgg gcattgttaa ggacgatctg
    18781 ccgctggctg ttgaagagca tgctaccagt aagataaggg acatcaatga tatttttgcc
    18841 atacatacga tgggattcag aggtgaagca cttgccagca ttgctgaagt atcgcagttg
    18901 acaattttgt cgcgcacaca tgatgaacca atgggtggaa agcttgtagt aacggataac
    18961 accacacaaa tgtttgattg ggctggtgct accgggacaa cagttatagt ggaaaacctt
    19021 ttttataata caccggccag gaaaaaattt ttgaaaagca tcaaaggcga actaaaccgc
    19081 atcaaagata ccatcttttg tattgcggtt gctcatcctg aaataaaatt caagattgtc
    19141 attgataaaa aaatgtcgtt tgtatttgaa cctgccgata gcatacaacg aattcagcaa
    19201 atatatggta atgaaattgc ctcaaattta attgatggtt cattacagga tttagaatgt
    19261 actataacag gatacatttc taaacctgag tactaccgtt ctaacaggtc attacagttt
    19321 ttgtatgtca ataaacggcc ggtgcagttt ccatacttca gttttatttt acaacaggtt
    19381 taccagacaa tattgcaaaa gggacagtat ccggttgctt ttatatgtat ggatgttaaa
    19441 cctgatttaa ttgatgttaa tgtgcatcct gcaaaacgag agatacgatt ttttgataac
    19501 aattatattc attccatgct gtataacttt gccaataaga ttatcagcgg cggtatccat
    19561 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaagacta tgctgtactg
    19681 ggggttattt ttggtgaatt tattgtaata caaaagcatg atgagctcta ttttattgat
    19741 tttcatgctg cgcatgaacg taaactatac gattatcttt tacaggtaaa tacgacagtt
    19801 gctgttcagc aattattgat accggttgtt attgagtttt cacccgacat ggttatgctt
    19861 gtcaatagtt accgtgatgt tttgcttgac tatggtttgt tgctggaaca atttgacgat
    19921 aattcagttg ttgtcaatgc tattcctgat ttttgtacaa ccagtgatat cgaaggattg
    19981 ataaaagacg ttgttgatag tttatatgaa aataaaaatc atattgataa tataaaaatg
    20041 actataattg aaaaaattgc ttgtcatgcg gcaaaaagag cacatgatag tataaccgat
    20101 gcagacatta ttgccattac agattatgtt tttacatgca gtgactaccg atgtcctcat
    20161 ggcaggccat ttgtgtataa gctgtcaaaa tcagaattag aggggtattt taaacggtta
    20221 tgaaatccaa aattatattg ggtatatcct cgtctattgc tgcatataaa tcatgtgatt
    20281 tagcacgtat gcttaccaga gatggttatg atgtcttttg cgttttaaca cacaatgcca
    20341 cgcatcttgt aacaccattg acgctgcaaa cattaaccgg caatccagtg tatactacca
    20401 gctttgcact tgagtggcgt gaaatgggtc atattgaatt aaaagacaat gcgcagttat
    20461 tattggttgc acctgctact gccaatataa ttggtaaatt tgccaatggc attgcggatg
    20521 atcttcttag cacaacattt ttatcggtca attgcccggt ggtgatagct cctgccatga
    20581 atcccaacat gtggaaccat aaagcagtac aggaaaatat tacaaagctt aaaaaatggg
    20641 gcgctatcat tgttgaaccg gacacaggat tggttgcctg cggtgatgaa gggcagggta
    20701 gattagcatc tattgaatcc atttataaca ttgcaatcaa tgcgattaga aaataaaaaa
    20761 ataattgttt ccggcgggcc aacgcgcgag tggattgacc ctgttcgcta tatctccaac
    20821 gcatcaagcg gaaagatggg ggttgctatc gcagatgctg cgtacaatag gtgtatcaat
    20881 acagttttca tccatggccc ggttgaacat tctatcatag ctaataaacc ctatcactgt
    20941 attggcgtgg aaactaccgt tgatatgctt gaagctattc aacaggaact atcgcccaat
    21001 actattctaa ttatggctgc tgcaccggca gattatgctc ctgttcataa atcgcctgtg
    21061 aaaataaaaa aaaataatga taccttaacc attgcattaa agcgtaatcc cgatatttta
    21121 aaaactatcg ctaccttgcg aaaggaaaat aaacagttgc gtgatgtaac actgataggg
    21181 tttgcagcag aaacaaccaa tattatgcaa tatggcaaaa caaaacttgt tgaaaaagaa
    21241 ttagccatga tatgtatcaa tgatgtaagc agtaccgatt cagggtttgg gagcgactat
    21301 aatgaagtaa ccattatgct tgcaaatgaa gaaacaatgc atctgcataa aatgacaaaa
    21361 aaagaagttg cggaaaatat actacaaatt attgaaacta tggtataaat taaagaatgc
    21421 aaatattgat tgtggtttat tgtattaatt tattacacag tgactatgca tgaaaaaatt
    21481 aattgttttg tttattgtat ttatttttcc agcttatgca tttgcacagc gtcaggtgga
    21541 taaaaagcca gttgtgcata ccgttaccat tgaatttaat ggtgagacaa tcaagggctt
    21601 ttattgtttc atagatgctc gtagcaatga agaaaaaagt tctaatgtag aaaacaaggc
    21661 attacatggg tcagttattt tgtttttgca ggggcatgcc cagcgagcag atgatgcata
    21721 taaatttaca tcgcagatgg cattaacctc aaaatcaggt gttgtgattg ttgtggtatg
    21781 tgatacgccc tacggtaaag accctcaata ctgcggtgat gcaggtaaag agattattgt
    21841 tatggcaatg atgcagtatg ctttaacctt gcagggtatt aatgtcattg atgctgagca
    21901 gatgccagaa gctataacaa taaatggcaa aacggtatcg atatcgcaaa gtggcatacc
    21961 tgcaacgctt acggtggttg ggtggtcgca tggtgcattg ttagcccggc gtgtggcaaa
    22021 cagatataac tgtgtcaaaa atcttgtaca gatatgtccg gctggtttca atgactggcc
    22081 tgataatagc tgtgccgcca attgctgtgt attatcagca ttttcatggg aaagcctgcg
    22141 tatatcaacc gaaatgttta aagggcatgc aaacgatgta tttgcggcaa gctggggtat
    22201 tacaaaaggg atagcgggcg atagttgtcg ttcatgtggt tcgtgcctgc atggtaattt
    22261 tacggtatgt aagtcattac gagcagtaaa agatatcacc gatgtaacac tacaagcaaa
    22321 cgcagataat ttcccggtaa ctactctatc atcgatagtt gttatatttg gcagcaatga
    22381 ttcactgttc aatccacaaa aacttttaaa ttacaaagaa tcacttaatc cccaggatgt
    22441 tgagcgtttc tggctgaagt attatcctga agcggtaaat aacaatgcaa ggatgaacct
    22501 ttttgtttta ccaggaaaac atttagcacc cgtactgtat tataaagaat atgcctttta
    22561 tacgttaaaa tatatcaatg aattgaagga atgattatac cagtgtagtt tgtagatact
    22621 tttttacatt gttgtttttt gcagtatcat ccatgacaat ggtaccctta tccagaacaa
    22681 taattctttg tgctacatgg tcaaccagat actttgaatg tgttgcaaaa actattggca
    22741 tggttaattg taacaaaatg tcaataactg catctgatga aggtttatct aaatttgcaa
    22801 gcggttcatc aagcagtaac actgaaggat tttttgcaag tactgaggct aatgctgcac
    22861 gcttcatctg tccgcttgaa agtgataggg gatgatgaaa ggcaacatca gcaaggttaa
    22921 cctgttttat tatatccatt gcttgtaaat atttttcttc atcatcacca ttgtttatac
    22981 ttaacatcac atcattgata atatccggca tgataaactg atattcaacc tgtgaaaata
    23041 aaatccccgt agattttcga atgagtattt tatttttatg ggtcaggttg ttatcattta
    23101 ttttcaccat ccccgatgtt ggcagcaata gagcggataa aatacataat aatgtagatt
    23161 ttcctgaacc attatgtcca acaactgcca ccctctggtt tccattcacc tcaaatgata
    23221 tgtcattgag tgcctgtgtt ccatcaggat agttatacga tagtttttgt accattattc
    23281 ttttcataat aaggtatacc tattgaaaaa ttccatttat tataataaga agcagtatga
    23341 taccaacagt aatatagtta gcaatagtgg tagtatcagc aagtttcatc acaggcaaag
    23401 aagtgatagc tcgtgataaa ataccattac gaaagtgtga cattgcataa aattgattat
    23461 aaagaaatgc aactgagtaa tattttggga tataatattt ttctttactt ttaaaattaa
    23521 tccgggattg aagctgaaac actatcatcc tgttgtgctg catcaaggta tacaatgttc
    23581 gtgtaaaaat aagaaaaaaa agcctcaatg ttgacgggaa aagatttata aaagctttca
    23641 atccattgtg ccctatgaaa aaacgtgcac caatgataat aatgctgata catacaatct
    23701 tgtatgctgt taatgctatt atataataat gaatactatc ccggataatg ccagttataa
    23761 gctgcattat aacaattgtc attaccaatg gcactatcat aatgagtgca gtgcgatgct
    23821 gcccggggat atagtgtaaa aaaataatag caagtactgc gcttgatgca gtccataaaa
    23881 aggaaaatgc attaagcaac gaacaaaaca gcagggatac aataactgca attatcttta
    23941 catagtggaa tgccattatt gtaacacagc atggataaaa ttaagttctg gttcatcaat
    24001 atagtgtttc atgacaaaac ctgcatcaag aagcaattgt tccagttcat ccttataggg
    24061 cataaaatct ttacttaccg gtgcatgtaa atgactatga aattcattga ggcttttagt
    24121 atcggataaa tgaaatatgt ataatgcacc atcatcggcc agtaggtcat gacatttttt
    24181 caaagccaat aatttatctt caatatgggg aaataccgaa aagcatataa tgaggttaac
    24241 gctttgcaaa ggcatgacaa tagtttcaag tggagctgca atgtaggtaa tattattaca
    24301 gtgtttatac tttgattgtg ctacttcaat catgccgctg gaagcatcaa tggcaatcag
    24361 cctccctgta ctcccaatat aatcttcaat aatggggaaa agaatgccag tgccacagcc
    24421 agcatctaaa acggtatcgc ctttctttat atgcatccat gacaacacct cagtcaattt
    24481 tttgatacgt gcttcattat taccagctgt agtatcccag cttttactta aattgttaaa
    24541 atagatgttc ttgctgtcat aattattatt taccataact atcgccaact ataatttttc
    24601 attaattttt ttatccccat aactgcaggc ggtacagtca tcaaaagcag tatgatgcca
    24661 ggaatacttt ttaacaggtc gtaaacacct aatagcgcaa atgaaatatg aaaataggga
    24721 aataccagcg catacagcat gtataaaaca aatcggtcaa cgatgattgc aatgatcatg
    24781 actatgtagg cattgacttc tggtgcgcga tgaattataa cggagattat aaaacaaaag
    24841 aaaaataatt cgatgaccat gagcagagca atgggtggat agagtggcgg cattccggtt
    24901 aataatgaag aggttatggg ggcgatagca cccgtaaaac atgcatagaa aggggtcatg
    24961 aaaaatgaac caattgccag cggtataaac attggtaaaa atagtgagcc caatcctaca
    25021 agatggaata tgataggaag agctacagca caggcagccg taattgatgc gattataatc
    25081 tgcgttattg taatctcttc atgcataaat ataaattata gatactattt tatgattaaa
    25141 aatatattgt ttgtaatact caattgatgg tgatttattt caagcagtat tttttgttgt
    25201 gataataaaa tttttcttga acataatgat gtttatgata gtattgaatt tagaggttga
    25261 accactgaac cactaaaaag gagaatatag tatgtcaaaa aaatttatac ccgattggac
    25321 cgaaaaagct ccagagccct attcatatcg ttctattttc aaatggggcg atccacattc
    25381 atttaaacat cccaatccaa aattatatca agaattaaaa acggtgttta atcttgacga
    25441 tagctatttt gcaaaaaaac acagcgaggg gagaaataca gttgcatata aaaaacctgt
    25501 aaggctgaca aaaaagcaat tacaggatat tgccgatatt gcaggctatg aaaatataag
    25561 cacaacgcag tttgatagat taaaatttgc aacaggtaaa accatagaag aagctatgaa
    25621 attacgtaaa ggtattgttg aacgagttgc cgattgtgtt gtccatccac gcagtgttga
    25681 tgatgttgaa aagcttatac agtattgcca tagacataaa ataccggtat atgtttatgg
    25741 cggtggctct tcggttaatt ttggactgcg aactgcaaaa ggtggcatta ctctggtact
    25801 atccacccat atgaataaca tactacatgt taatgaaaaa aatcaaacgg ttactgtaca
    25861 ggctggtata cagggacctg ctctggaaga agcattgaac aatgctaaaa agcattttgg
    25921 tacaaagtat aattatacct gcgggcattt tccacaatcg tttgaatatt catccattgg
    25981 gggctggatt gtaacattgg gttcggggca gtcatcaagc tattatggtg atgcctatga
    26041 tcttgttata agcatgaagt gggttacccc tgctggtaca tttacaacca gggattatgc
    26101 tgcaacagca accggtccta aaataaacga tatcatgaaa gggagtgaag gcacctttgg
    26161 cgttttagtg gaggtaacat ggaagatata tcgttatatg ccacaaaata gaagaagatt
    26221 tggttttatt tttaaaacat ttcaggatgc catcaatgct tcgcgtgaaa tatcgcaggg
    26281 tgagtttggt atgccagcag tattcagggt ttcagatgaa gaagaaacgc atataggttt
    26341 gaaactctat ggcatagatg gaaccattct ggataaacta atgcagctac gcggttttaa
    26401 accaatgcag cgttgccttt ttataagctc agcagaaggc gaaaagtatt ttaccaaaaa
    26461 cataaagcga caggtaaaga aaatttgtaa aaaatacggt gggatgtaca ttacaggatt
    26521 gccaacgctt atgtgggagc ccggacgata taaggaccct tatatacgag aggatttgca
    26581 tgattacggg atactcattg atacactgga aacaagtgta aagtgggata acctccatga
    26641 tgttcataag gctgtgcgac agtttataaa aaacaggcct cagacagtat gtatgactca
    26701 tgcttcgcat ttttatcctc agggtacaaa cctgtatttt atctatatta tgaaaactga
    26761 tgatatgaac gaatacatta catttcagga tggtattatc gatagcatag caaaaagtgg
    26821 tggatcattg agccatcatc atggtgtagg gcgaatgtta gcaccatgga tggaagagca
    26881 tattggcaaa gaacagatgg cagttttacg agcaataaag aagcattttg atcccaatgc
    26941 tattatgaat ccgggtggac agctgggatt ggatttaaaa ggcaaagatt ggcgctctat
    27001 aaagtaatat acatcgtatg caataaaggg cagtggtata cttcccttta ttgtaataat
    27061 agatgaatag cactggaatc ctttgctata gaataaagta aattttggcg atagttcata
    27121 ttaattaaaa ggggcaacgt aaacagttgc ccctttttgt gtatatttta aatctattac
    27181 tattaaaatg tatggctaaa atcaaccgat attgatgtat cctcttcact tgtaccataa
    27241 tagaaatgaa ctatggttgc catattccat gctatgacca aaccgccacc ataaccctga
    27301 tgatagtcgc caaaacgggg atcactgaat ggatcgcctg cctcatcgta tacatttcct
    27361 acatcataaa atcccgttaa tttaaactgg aagttctgac cccatgcatt cgtttcggca
    27421 aatttccagc gcagttcagc attccctacc gtcattgttg gcccaacaaa acgttgctcg
    27481 cgatatcccc gtaaggtcct gttgttgcca agccctgttc tgcggttgag cgaaaatcca
    27541 aagtaaccca tctcaaagaa tggggtgtca ctgttggtat cagtataggc tgcacgcaat
    27601 gccagcgtta aacttgaaag caatggtata taaccctgta attgcacggt gttacgcata
    27661 aagtcataat ctgagccaag tacattatca gatatttcat acgcataatc aatcaagtat
    27721 cctcttttgg ggtcaggttc ataatcacgg gtatcgtagt aaataccagc acgaacaaag
    27781 ttggaccatc caccatcgta tccctctggt tgccattgtt tcaataatgt tggtccctgt
    27841 ctatcgtggt caaatttatg attatcccat gcgtctatat ctactttttt aacctcaatc
    27901 cccaccagta tcttcaggtt ttgtgttaaa tccctgtgaa cattcaggaa aaagctgggc
    27961 ttggtatact tatagttgta atatttttgc aaattatcat tatcatcagc ataatctgta
    28021 taatcttcgt atttatcaaa cttatgatag ttagtcccat catacagtgt cagcttttcc
    28081 tttgcggtat cagcgccaat gccaaagaag tttgcgttaa tctttttatc ataggcaaca
    28141 gaggttatta ccctgaattt tgtgcccatg atatacggca tatcaagatt aacctcatgg
    28201 tactgatagc cattggttgt ttgataaaac tgaccataca actgcatttt atagggtgca
    28261 taggcaaaat tttcttcatc cctgcttcca ttattataca tatacacccg tactccatat
    28321 ccaaagccat tatcgctatc ataatttacc agaggcaggc ccgttgggta ccagccttct
    28381 ttcttttttg ctaaatcctc gtcggaaagc tttgctgcgt aagtaaaggg cagcgatatt
    28441 gccaacgaga gtattacaat gagaatgcag aaaaaaattt ttttcattgg cttactcctt
    28501 attttgctat aaagtatagt acctttgtaa taacgatatg tattgtaata taaaagtcaa
    28561 gcagaaattt atattaatcg ttatcgatgt attataaaaa aagtaatatt ttatatttca
    28621 attacttaaa tgcatacaca accatagagc ttgttaaagc acaattgcca gcatggattt
    28681 tactggcata gtgatagagt gctatcctta gccagctttt ataagcatat atggaattaa
    28741 aattcatcct tgacttcatt agttttggaa gtaatccaaa atctactggc tggaatactt
    28801 taatctttgt aaaacctatt tttttaagtg tgtgggtaag attatctttt gtaaaatacg
    28861 aaaagtggcc cggtaaaaaa tagccatagc gctctttttg tattgcagcc tgcagtccat
    28921 ccatgtttgc agtttgaata acaagtaaac cgtcttgttt acatagatta tagcattcag
    28981 tcagggcttc atatggctgg cgcatatgtt caagcacttc aattaatgta ataactgaaa
    29041 aaaaattatg tttaaaagga tgatcaaata atgttccaat atgtataaca tcgctcccaa
    29101 atcgctgctg tgcatatttt ccagaatatg ctgatggttc aatcccatac gtgatataca
    29161 attgagatgc tgcatgtaaa aagccaccaa atgagcatcc gacatcaaga aaatttccac
    29221 ccggtacata gttgtgtatc gtatgcaacc gtgcattcca tacatactga gaataccgct
    29281 cggttattcg ttcatcgtaa tatgaataat ctgatgtacc ggtataatat ccttcatcat
    29341 ataaagtatg ttcagactga tgactgaggg gagggttcat aaatataaat ttacagctat
    29401 tacatttata aacagtaaat gctggattac aggtaactat cgcatataaa aaatatattg
    29461 cattgctatt gcaaagcgga catgatgtaa gaggactatc aaatgtaaat gttttcatac
    29521 cgttgcgtaa aaataccaat gggataattt tttatttaga tcatctaaaa cagtgcgttc
    29581 cattaaacca atatgaaatt ctttaaaaat agataaatat ttttttacct catgtaacga
    29641 ataaagcgaa atgacagcac cctgtatatc cggtagtgat gtttcccaca tattattccc
    29701 aagatgcttg ccccgtgcaa tgtaggtgtc gtgttcggaa cgcaaagttc catatacaat
    29761 gccttcactg gataaaactc gcttaatctc ggcaagcatg cagggcagca gttctttttt
    29821 tgcatagtgt aatgagcccc atgcaatgac tgcatcaaag gaactatcgc cgaaaggcaa
    29881 tttgttaatt gaacattgta tgaaaatatt atcaagcaaa ctttgcgatt gcttcaaagc
    29941 attgtagctt atatcaaccc caataac
//
LOCUS       DAOOJH010000022        28865 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1712_length_28865_cov_9.887053, whole genome shotgun sequence.
ACCESSION   DAOOJH010000022 DAOOJH010000000
VERSION     DAOOJH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28865
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1712_length_28865_cov_9.887053"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            518..1030
                     /locus_tag="PLJ75_04855"
     CDS             518..1030
                     /locus_tag="PLJ75_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04578.1"
                     /translation="MRNSTLALVFMLCFFIGCATIFSSKNKTISIISKPEGADVSVYS
                     SQGNKIYEDKTPCSITMKKEQLLDAKVVIKKPGYKPGEVNLGRSIESWGLANACCLIP
                     GIIIGGGIDLFTGYLLKPEITEINIILEKEDNKTESKQGNNNTHYEKIEYRISILNDH
                     DTVNIDINEM"
     gene            1168..1638
                     /locus_tag="PLJ75_04860"
     CDS             1168..1638
                     /locus_tag="PLJ75_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HPD04579.1"
                     /translation="MKKGLSILVLFILAAATSIAEAGVVRNNAGCGVGTMIFGDKDGL
                     LFEILATTTNGICGNQTFGMTSGTLGCAPMKGIVSNEKINLYVADNMDNLAKDIAKGN
                     GEYLETLALLMNVPESEKQVIFTKLQTNFNKIYTSSDVTSTEVVKNIEVVLQNS"
     gene            1673..3637
                     /locus_tag="PLJ75_04865"
     CDS             1673..3637
                     /locus_tag="PLJ75_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPD04580.1"
                     /translation="MLPYIRHDLKKAIVPFFIFILCCYFLLYTNSIFAQQYQSTTDDQ
                     KTDIISQELFANINVQQLITLANSRKLSQSKNWWALLHYKKNISGTKSLVDDPKFFLA
                     LDGKYNPESELEATIRYLYSYNDKENPVCRFMARYAWLKKELGVTSPYETYCEKLQEI
                     ISFKDDMFVHLIFPTGYINSPASMFGHTLLTVQSQYNPGLLSSAVNYAANTNETFGPS
                     FAFKGIFGLYRGYFSIQPYYSKVQEYSDLESRDIWEYKLNLNQDEVRMMLFHIWELDN
                     IYSDYYFFDENCSYNLLYLLEVTRPDTHLTDNFFFWVIPIDTLRKVSNAGFIENKAFR
                     PSRTTLIQYMVGHLNKQQLNAIKSIAKGNNPLDVISHYSFNDLEKVQVLDCASEYIQY
                     CYADKQYPQKEYQTIFINVLKLRSTIPNTITYSPPVPPPPEDGHYSSRMSIQMGSFEN
                     DTFSELSYRPAYHDLCDDTSGFVSGSQIIFGEIAARYYWDTQKIQFQRFDIIDIESIA
                     PINSLIKPLSWKIITGFERLPFENNNELLVYQLNTGGGYAFSLTQWLQLYGMAEFSGL
                     LNNDFKNNIEIAPGASIGLLLQPFNKMRLRFHVSDYYYIFGESFNYVAIKTIVQYNIN
                     QRFSFRFDVNYWHHEQWFNQFSGSFIYYFW"
     gene            3646..4458
                     /locus_tag="PLJ75_04870"
     CDS             3646..4458
                     /locus_tag="PLJ75_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD04581.1"
                     /translation="MNYRIYIILFFSIFYCSCLNKVIYYPDNRVAIRGNIPFPIEDVH
                     IKVNDEVTLHGWFVFNNDAEFTLIYFHGNAGNITNRVEKINILYKIPLSVFIIDYRGF
                     GESSGKPSEDGLYADALASYDFVINNKQLSPDSIIIYGESLGGAVAAYCATKRNAAGV
                     ILDSAFTDIYSMVKHHSACFMYIFFSEKYPTIDYMQSIRYPVLILHSKDDEVAPFAMG
                     QALYNASLSKQKQFVPLHGSHNANFIVDKATYVNSIQSFIQSLKIASKEHGQ"
     gene            4455..5015
                     /locus_tag="PLJ75_04875"
     CDS             4455..5015
                     /locus_tag="PLJ75_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04582.1"
                     /translation="MKKWIAFTMLLILLSVTGTYSEDCNGISPAALRLLAESTIDPCS
                     ICQKKLQRQAYAIVARELFPGKKLTFASTCPLAKTNDCQDNELIMQNSGYPEKVVIKG
                     KEAAMPEVFYKFFSQDKHIAGIAPDDYTSNSIQTMYKTLKINSPVEAQLEIISYNYSD
                     CPSALYLHDRNALIIFCKILSMKQRQ"
     gene            complement(5018..6205)
                     /gene="glpC"
                     /locus_tag="PLJ75_04880"
     CDS             complement(5018..6205)
                     /gene="glpC"
                     /locus_tag="PLJ75_04880"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HPD04583.1"
                     /translation="MENISFDHCIKCTVCTIYCPVARVTHLYPGPKQSGPDTERLRIK
                     NPELLDISLKYCSNCKRCEIACPSDVKIADIIQTAKWRYSKKELRIRDFLLSRTDLMG
                     TFGTLFSVIVNFFTHLGIVKYFLDVIFKIPSGRTFPNYASGTFTRWFKAVRKQQSTYA
                     DKVVYFHGCYVNYNYPQLGKDVVKVLNALGKGVILTKEKCCGVPLIANGYIKKAQSHG
                     RHNIKVLSSALSDEKTKIVSASSSCTLALKHEYPNLLELDNSPILNTIAYITQFILEE
                     FEKGNILSLQPLSYTVAYHSPCHLERMGSAAFTIELLRKIPALTVKILHSECCGISGT
                     YGFKNEYYGISQSVGNELFRIIEEAKPDFVVTDCETCKWQIEMNTKYKVVHPISLLAE
                     AIE"
     gene            complement(6218..7513)
                     /gene="glpB"
                     /locus_tag="PLJ75_04885"
     CDS             complement(6218..7513)
                     /gene="glpB"
                     /locus_tag="PLJ75_04885"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HPD04584.1"
                     /translation="MRYDCIIIGAGVAGLTAGIRCAKEGLHTAIISSGMSALHFSSGS
                     IDLLGYRDNTVVYKPFEAIDQLTGTIAQHPYTKCGKPLIIEALLFFKQECELQGLFLF
                     DNGNLNHFHVTTLGTLKPTYFSQRSVFNEEVYSRFKAMKKIVIINFEGYRDFHPQLAA
                     AQLSKHALFANCDIVAKQIEMPQLQDDGKHPHEFRSVDIARIFEKEDNIEKIADVIRL
                     AADDADFVVMPAVMGITNFNQIHNKLQSRTRLLVYEVPTLPPSILGMRIDNALKSRFA
                     ELGGVYIAGDKVVRADITDDVVNAIYTNSHPENPMEAQCYILSTGSFFSGGLVSEFNN
                     MYEPIFKLKLHHEPERNKWYNQNFFHSDSHGFLQYGVLTDESLHPYDSNNNIIQNLYC
                     SGAIMAHYNPIKEGCGSGVAISTGYKAAGQIVNLLKHMS"
     gene            complement(7510..9135)
                     /gene="glpA"
                     /locus_tag="PLJ75_04890"
     CDS             complement(7510..9135)
                     /gene="glpA"
                     /locus_tag="PLJ75_04890"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HPD04585.1"
                     /translation="MKTLEAEVIVIGGGATGCGVLRDCTLRGIDAILIEKNDIASGTT
                     GRNHGLLHSGARYAVRDLESATECIKENRILKKIAWHCIEPTGGLFITLPEDDPNYHN
                     RLLEACKFAGIDAKEISVKKALSIEPNCNPNILSAVWVPDGTIDPFRLTSANILDSIE
                     RGSKVCTHTEVERIMLKQNQVIGVQCYDKTAGEYLEIYAPIIVNASGVWGQHICKTAG
                     IELKMFPSKGSMVIIDYRINNVVVNRCRVPSDGDIIVPGDTVSLIGTTSRKIPYDTID
                     ELVVDDDEIDVLLSDGEKLIPNVSRARVLRAYCGVRPLIAVSGEMDGRDISRGVVLID
                     HKERDNVQGLITIAGGKLMTYRLMAEMTTDLIAKKLGNSKRCLTHKIPLPGSEKRVGY
                     TKKIKYFSGIPNSVVGSTHYRHGERVHNILSLKKRNYGLLCECEMVTLGEVEYAIKNL
                     KVNDIVDLRRRTRIGMGPCQGELCAYRAAGLFTEFGDFTPDESTKLLRDFLEERFKGV
                     KPVLWGDGLREFEFTYWIYQGLFGLGLVEDNNT"
     gene            complement(9312..9938)
                     /locus_tag="PLJ75_04895"
     CDS             complement(9312..9938)
                     /locus_tag="PLJ75_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04586.1"
                     /translation="MKAELRRQHILDCAKQVFAEKGYYEAYVEDVIKVAHIGKGTIYE
                     YFRNKEDLFLSLLIRSLEEWKKAVNISHEEMKQLNPISYFHYRIQRSLMFLNDDRLLT
                     SIILRTGFGFNPHIQKLIGEFEKNLRKHILHDIRLAKHFGFLRDDVDLQLTTTILIGG
                     IIMIAYHDILNVPNLSKAQIDVLTDKYIDTFIKGLFKMQNVNQYINSQ"
     gene            complement(10033..12891)
                     /locus_tag="PLJ75_04900"
     CDS             complement(10033..12891)
                     /locus_tag="PLJ75_04900"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04587.1"
                     /translation="MILTLIHNITLLVALSVIWSPILTLRKNYPNTYRILSGLLFGFS
                     AIAAMMTPFHFEEDIIYDGRSVVIALAGLFGGGVASFVASGVAVFYRIYLGGAGVYAG
                     VASIIVSALSGLAMRRIYKNEPSEIPLLGIIIFAVVTHVMVLSCQLLLPWPKGLTVIT
                     TIGVPYILVLSSGLIAIALVFTTVEKRTLALQHLQAGADLLQDVLSVSPSVAFIMNPI
                     DNTISWASPNVEIVLGYTPDEMMHANWFEESIHEDDISQYYSLFDTLTIENFASAECR
                     IYDKSKAIKWVDIHLHAVRYDDGIISALFGSLSDITQRRQIAIELEESEERYRTIFKN
                     SKAMMLIIDPADGRIVDANDIAVQYYGWTKDELQSMNIHQINTLPPDEIKARMEEART
                     CKRVYFTFKHRKKDGNIIDVDVYSSAITIKGKQYLFSIVNDVTAKRQAELSLKKSEEL
                     FRLIFENAPVGIYAFDAYGVITACNDNFITIIGSTREALIGLDTLKLPDEKIVKATKR
                     CLQGEQAEYRDWYKSVTANKVTPVRVLSSPLIVEDTVVGGLAIVEDLSQQIDAEKQKE
                     KLEEQLRQAQKLEAIGRLVGGIAHDFNNILSIISGYAALIKFKIDRDNIIYPDIEEIA
                     HAARRSADIVKQLLTFTRQQKVNPVRLNPNEIIKTSEKMLAAMIGENIKLRLELAEDI
                     YDIWVDSGMFIQILTNLASNSRDAIKDTGTIKIVTQNKFLDDTFCATHPGCKPGDYAM
                     IQCIDDGSGMDKDTLERLFEPFFTTKEVGKGTGLGLSTVYGIMKQFSGHITVYSEYGQ
                     GTVVNIFFPKYTEQMPEIPEISAIENNTKTITVLHNKIIVVEDDTAILGMLQRMLLLL
                     DQDIMAFNDPVDALNAVDAYKDFAILITDVIMPEMNGVELYEKLRRIIHNLKVIFISG
                     YSNDAFNDIAFNVENSYFLQKPFTFEDLKMALYTISIK"
     gene            complement(13039..13353)
                     /locus_tag="PLJ75_04905"
     CDS             complement(13039..13353)
                     /locus_tag="PLJ75_04905"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04588.1"
                     /translation="MRLKQSDIECIKQSFIEVFDEGEIYLFGSRTDDTMRGGDIDLYI
                     VPGNTDSLTQKKFKFLVALKKRIGNQKIDVVIKRGSERSIDVVATKEGILICRHYRDM
                     NF"
     gene            complement(13560..15071)
                     /locus_tag="PLJ75_04910"
     CDS             complement(13560..15071)
                     /locus_tag="PLJ75_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HPD04589.1"
                     /translation="MEKLTMLIQKIERKHIIAAIGIVIAVSLVMYLIGVLGPTEPKLV
                     LVGAIPDNEYDPAEWGKVYPLEYESWQKTKEPRPTGKSFYKKGWDTDKVVYDKLSEYP
                     FMALLFKGWGFGIAYNEPRGHWYMRIDQDEIDSSRVKAGGVCLNCKSPYMDALVKQYG
                     NSFFSMPWADAIAKIPVRHKNLGASCIDCHDNKTMSLKLNKEIFKRALAKLGKDEFSR
                     QEMRMLVCAQCHVTYNIPKNEAMQSIGLEHPYDGSRWGNISIENIIKLIKKNTHWGEW
                     KQEVTGFKVGFLRHPEFEFFTRQSVHFNAGLACADCHMPYKRVGSFKISDHNLMSPLK
                     DDLFACLKCHPQSNERLVKQIKEIQDRTLSLLLKAGYHEAVVAKLFEKIHTQFKDGLQ
                     NDPLYITAKDYYLEALYRLIFMGAENSIGFHNPEEAGRILGDSIGFSTKAEGYLRQLL
                     AKKNVDVGNSINLELAKYLNNRGQHKLMFQKQQEFNDPFGTVEKTVPRASLGL"
     gene            complement(15098..15526)
                     /locus_tag="PLJ75_04915"
     CDS             complement(15098..15526)
                     /locus_tag="PLJ75_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HPD04590.1"
                     /translation="MKYKLYSVFGLLFVMVVCAVYGTAKFMQKTSMPQYCGSCHVMIP
                     QYEDWFYIGKHTQITCVDCHLPHNNDANYLLWKGLDGVKDVVMYYTRLYPEIIHSSSH
                     AKKTIQANCIRCHCEMVSRIDNGAMQCWDCHRYFYHSIIH"
     gene            complement(15529..15852)
                     /locus_tag="PLJ75_04920"
     CDS             complement(15529..15852)
                     /locus_tag="PLJ75_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD04591.1"
                     /translation="MIDEALKNSCVLVNFIDYQNSAIVSKTLIQNSGGTITLFAFDKA
                     QSLSEHTAPFDAFVMVLDGKFTITIGGQGHTVAVGEAIIMPANVPHALIAIEKAKMLL
                     VMIKE"
     gene            complement(15952..17148)
                     /locus_tag="PLJ75_04925"
     CDS             complement(15952..17148)
                     /locus_tag="PLJ75_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HPD04592.1"
                     /translation="MSEFLSDMGQKKEQLKKMIKMLHDGADIEQMKILFKKEFGNVMP
                     HQISMLEEELIKEGMPAEEVHRLCQLHIDLFRESIDAGRVDVPLGHPLYILYTEHSRL
                     LEFAQNLVESATKLIDAPTPDVFARLQQLVSFFKESSVHYLREENVIFPVLEKYGVTQ
                     PPKIMWMEHDTIRAIENNIYETADLLAKNVTMELSQKLQMLSIQLSETLVDHFYKENN
                     ILFPTSYKFFNDDDWELVKQEFDAIGYTTFSPVADISKRGGMTTRMEAGSIELETGKL
                     TLEQLTGMLDTMPVDFTFIDKNDVVCYFNNAPDRIFVRSKAVIGRTVQNCHPDKSIHV
                     VNKLLEEMKQGTRDKAEFWITLNGNLIYITYYAVRKNGEYMGCLEVTQNITDIQKITG
                     QKRLLD"
     gene            complement(17161..17520)
                     /locus_tag="PLJ75_04930"
     CDS             complement(17161..17520)
                     /locus_tag="PLJ75_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HPD04593.1"
                     /translation="MDANNQNAIKHDIGVLGKFINIYCNGKHPARAKSLVHGHGKVAL
                     YVNPLEVQLCDECTKLLLYAASKRILCPYNPKPSCKDCTTHCYAEPKRSTIREVMRYS
                     GIRMIFRGSISYIKKFL"
     gene            complement(17529..18119)
                     /locus_tag="PLJ75_04935"
     CDS             complement(17529..18119)
                     /locus_tag="PLJ75_04935"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPD04594.1"
                     /translation="MDTIHLDENLIAILDKITEIKRSMLWEISETYGLTPLQIQILLL
                     IKRCAKYKKINPTDIVKELYVSKATASSAVKTLLKKGMIEKNINSNDNRSFYVSLTKK
                     AGFIITHIEQTAHTMQRYLSQIDKNDKRIAFGVLARMVMAMQDDGAIDYIALCINCEH
                     CKQLSSNTFMCSFTKRTFQYDGINIGCCNFTDKKII"
     gene            complement(18317..18676)
                     /locus_tag="PLJ75_04940"
     CDS             complement(18317..18676)
                     /locus_tag="PLJ75_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04595.1"
                     /translation="MDNKEKIALIRKGNEYFNQGKIKEAIELFVKTGYKDGLHRIGDY
                     YYYDKRLPLIALKFYRLSGSIKKIDEIMERMVFALGKWLGNDTVKESPYRVKLPPLKV
                     SPKLKMLAQEILEKNKE"
     gene            18970..19179
                     /locus_tag="PLJ75_04945"
     CDS             18970..19179
                     /locus_tag="PLJ75_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD04596.1"
                     /translation="MGHIVIDSNRCKGCYFCITYCPKQILVISKNHNIQGYFPAELAD
                     DKKEHCTACKICALMCPDTAIEVFK"
     gene            19170..20219
                     /locus_tag="PLJ75_04950"
     CDS             19170..20219
                     /locus_tag="PLJ75_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPD04597.1"
                     /translation="MQMRVLTKGNIALAEGAIQAGCRFYFGYPITPQNDIPEYLAKNL
                     PKVGGTFIPAESEIASINMILGASASGARAMTSSSGPGISLMQEGISYLAGSELPAVI
                     VNIMRIGPGLGGIAPTQGDYNQAVHGGGHGDYRNIVLAPASCQDMFDLAIKAFELADK
                     YRNPTMILADAMVGQFQEACRLTPEKPVIIPDKPWALTGAQGRPAQFIKSLFLGPGEM
                     EIHNLDLKKKYDTIQQNEVLYETRHLDDADIMIIAFGTPSRIAKTAIHLLREEGIKAG
                     LLRPVTLFPFPANAINEYSKRIKKVLVVEMNTGQMLRDVQLSCTADTAVYFYGRPGGG
                     IPFPDDIMREVKKIM"
     gene            20229..20981
                     /locus_tag="PLJ75_04955"
     CDS             20229..20981
                     /locus_tag="PLJ75_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD04598.1"
                     /translation="MQAVFTRPKTLKDTPTHFCPGCTHGIAHRIVAEVIDELGIGEKT
                     IAIPGVGCCVFLYNYLDIDVLEAPHGRAPACATGMKRANPGRIIFAYQGDGDMAAIGT
                     SEVIHAANRGENITVLYINNTVYGMTGGQMAPTTLLGQHTTTTPYGRNFRNEGYPIRM
                     AEMLATLEGVAYSTRVALHKPKYVMQAKKAVAKAFTMQINEMGFSMVEMLSTCNTNWR
                     VSPTEAIDKIENEMIPYFPLGVFKERTGKDFL"
     gene            21381..21917
                     /locus_tag="PLJ75_04960"
     CDS             21381..21917
                     /locus_tag="PLJ75_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPD04599.1"
                     /translation="MYYDIIMAGFGGQGIQIISQLVAVAAVNKGLEVTYLPSYGVEKR
                     GGRTNVFLVISDREIGSPVTNHPRALLVMDQNSFDTYIPMLRPDGLLVANGSLINTSS
                     YKRDDIVSCCIFCNEEAIKLGNPKLANMIALGAFLKHINFLTIDDIGNIMNEVIPERL
                     SHTIGPNMQALHYGNTIA"
     gene            complement(21914..24268)
                     /locus_tag="PLJ75_04965"
     CDS             complement(21914..24268)
                     /locus_tag="PLJ75_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04600.1"
                     /translation="MDEKRNINFSFLDCIQQPSIIINRNGTIVFANNSFCQNYSFNED
                     KIKNASIEEICSNHEVLFDAMVSAFSIKGKINIKELSLKNNNILIHGICVPFDSEHVI
                     LYSDVSDGELLRKSKMLVQTILDTIPRQIVIVDHNYSIIMANSEWKRFVNEVLEIPGD
                     SLHHDIIELCDKGGCLYDETKSRFSDAIRAILSGRQSLISFESKIYHNENTRWFQFNF
                     FGYTLNGIKFCVIIFTDITDHKVYQEIIQQQRDTFQKYLDISPFMIVLVNPEGKITLV
                     NSKACEILGYTSEELLGKDWFDTCLPHDVAIDVKKTFIELMKGNIKPVEFYENPIVTK
                     SGELKLIAWHNTIIYDANGTILASMSSGEDITQKKQMEIELLKSSTNLKAIVKAFPDI
                     YVIADKDGYIVDYQYGDEKILYATREETLGKTFYDVMPTHIATLYASAVDDAMSTNTI
                     QSIEYTIQIGDESRWREARLVPVIKNRVLAVIRDVTQRKNAELALIESGKRYRNLVQR
                     IPAAIIEMNIQGDIIFINDYFEKITGFTLSDLKGTHWFRSMIHPQEDKRKIRQFIEQI
                     QQGDVSNFEIHIINKEGEDTYLTITTSNIYNKAGKLERIVCIATDVTPIIAMRENLRE
                     MAIKDELTGLYNRRGFMMLAEQQMKINRRSELGMAIFYVDMDNMKLINDTFGHHEGDK
                     ALIDAAAILRLCFRDSDIIGRMGGDEFAILAIDCNRHDIEIMQNRLIENINEFNAKSL
                     REYQLSLSIGSIFIHDSSGNIEDILVRADKTMYRNKLERKKQRH"
     gene            complement(24516..26399)
                     /locus_tag="PLJ75_04970"
     CDS             complement(24516..26399)
                     /locus_tag="PLJ75_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD04601.1"
                     /translation="MATGVMSLNPLVDTRDTRFVLFEVLGVDKLTQYPMFADFDKDTF
                     EDVLNLAETIAVEQVYPANMEGDKIGVQYEPDTKKVILPECYKPALQAMYDAGFIGLA
                     NDPEIGGMGMPQAIGQCCTEFFTAGSVAFSMYPALTIGALNLIRNFGTQEMKDLYIPK
                     MITGQWTGTMCLTEPDAGSDVGNLKTRAIKLDDGTYKIIGQKIFISGGDYDMPENIIH
                     PVLARIEGDPPGTKGISIFIVPKYRVKADGSLEFNDVVCSGIEHKMGIKASATCSLSF
                     GDTGDCIGWLLGKERQGMKIMFQMMNEARLYVGLQGLAVSSAAYMHAITYARNRVQGV
                     HVSQMLNPDAPKVSIIQHPDVKRMLLWMKSYVEGMRLLTYYVGYQEDLAHALDGEAKK
                     EAQAMIEILIPITKAGNTDNAWKITSEAIQVYGGYGFCSDYPVEQFARDSKILSIYEG
                     TNGIQSMDLTMRKILMNPEQYNYSVFKKRVQETIDRAKGVVDDKYVDVVQKGLEKVDV
                     VISYLKDQMAAGKFLHIFASATPLQQAMSMFVLAWMHLWMLSLTQPKMKELIGDKKGD
                     DLNALLADNSEAAYYNGKVLSSQFYLGAEFKKFFGMVDYIIDGEAAVVKASEHIFTGA
                     PLE"
     gene            complement(26628..28499)
                     /locus_tag="PLJ75_04975"
     CDS             complement(26628..28499)
                     /locus_tag="PLJ75_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD04602.1"
                     /translation="MALNPLVDSRDVRFVLYEMMDVIQLCKLPKYSAFDKDMFEDMIN
                     LAETMAVEQIYPTFAEGDKEGCNYDGATKKVTIPKCYHPALDAFYEAGFGGMDIDDEW
                     GGMGIPQVIAWACNEYFCAANYPLLMYPGLTHGAAELIEAFGTEEQKHMYLANMYSGK
                     WGGTMCLTEPDAGSDVGALKTKAVRQSDGTYKISGQKIFISSGDNDYYENMIHPVLAR
                     IEGDPPGTKGISIFIVPKYRVNADGSLGEFNDVNCAGIEHKMGIKGSATCTLSFGDNN
                     NCIGWLLGEERKGMKIMFKMMNYARMGVGLQAHSTSSAAYMHATTYSKNRVQGAHVTQ
                     MLNPDAKGVTIINHPDVKRMLLWMKSYVEGERAMIYYLYLNYELAQLLEGDAAKEAQG
                     LVEIITPIEKAGCSDMSVLITSEAMQCYGGYGFVADYPVEQMMRNSKITAIYEGTNGI
                     QSMDLAMRKILMNPEQYNYTILKKRIADTINKAKGIVEDKYIALVERGVQELDKVIEM
                     MKQQMAAGQFLHLFANATPLQQAMYMLLIAWMHLWSLTITLPKMKELVGDKKGEERAA
                     LLKDNADAAFYSGKVLSSQYYLGAEFPKYFGKIEALLGGESAVIKASEEIFTGMLNE"
BASE COUNT         8953 a         5906 c         4608 g         9398 t
ORIGIN      
        1 gactttgata catattccac aactgatgtt atgtatgcca tggagagttt aaacagtaga
       61 ccacgaaaaa gattggggtt ttttactcct gatgaattat tttttacaga aaagggttgc
      121 acttaattgt tgaatctaat tagttttatg aaatagcatt acaataaaat ataaaaagaa
      181 ttgtattgtt agtgcaatat acttttttaa agcaacagat attttactct tcataaaagg
      241 caacaatatt gccaatgtgg cttattttat tataatacag ttaataaatt tatatttttt
      301 tatatgctta taataatttt cttgacataa tttaaaccat ttatttactc ttatgcttga
      361 gcagtacgtt agcgttttgg aaggaaagta taaattcatc cgtatcaaga aaatcatata
      421 ttaatctacc atggatataa caaacggcaa taatttggtc aaaatcatga gatgctgttt
      481 ttatacatac tataaataaa tatataggag gcagtctatg cggaatagta ctttggcatt
      541 agtttttatg ctttgctttt ttattgggtg tgctacaata tttagctcta aaaataaaac
      601 aatatcaatt atatctaaac cagagggagc tgatgtttct gtatattcat cacagggtaa
      661 taaaatatat gaagataaaa caccctgttc tattaccatg aaaaaagaac aactattaga
      721 tgcgaaggtt gtaatcaaaa agcccggata taaaccaggt gaagtcaacc ttggcaggtc
      781 aattgaatca tggggattgg caaatgcttg ttgtctgatt cccggtatta ttattggagg
      841 tggtattgat ctatttactg gatacttgct aaaaccagaa attaccgaaa taaatattat
      901 tttagaaaaa gaagataata aaacagaaag taaacagggt aataacaata cccattatga
      961 aaaaattgaa taccgtattt ccatactgaa tgatcatgat actgttaata ttgatataaa
     1021 tgaaatgtaa tgggatatat taaagtaaat ttgttctatt gtactatgtt taatgtaaaa
     1081 tttttcttga cagcttttta tttttatgta tcaataaagc aatgcaaaaa attcccctat
     1141 ataactaaac tcttgatgga ggtttttatg aaaaaaggtt tatcaatact tgttcttttt
     1201 atattggcag ccgcaacaag catagctgaa gcaggtgtag tacgaaacaa tgcaggttgt
     1261 ggtgttggaa caatgatttt tggcgataag gacggattgt tgtttgaaat tttggcaact
     1321 actactaatg gaatttgcgg caatcagaca tttggtatga cttcaggtac actgggttgt
     1381 gctcccatga aagggattgt ttccaatgaa aaaatcaacc tttatgttgc tgacaacatg
     1441 gacaaccttg caaaagacat tgccaaaggc aatggagagt atctggaaac attagctctt
     1501 cttatgaatg ttcccgaaag tgaaaaacag gtaattttca caaaattgca gactaatttt
     1561 aacaaaattt acacttctag tgatgtaact tcgaccgagg ttgttaaaaa cattgaagta
     1621 gtattgcaga atagctaaaa aattatattt aaaaattagg ggaatattgt cattgcttcc
     1681 atacataagg cacgatttaa aaaaagctat cgtgcctttt ttcattttta tactatgctg
     1741 ctatttttta ttgtatacca attccatttt tgcacaacaa tatcaaagca caacagatga
     1801 tcaaaaaaca gatataatat cacaagagct ttttgctaac attaacgttc aacaacttat
     1861 aacgctggca aattccagaa aattatcaca atctaaaaac tggtgggcgc tgcttcatta
     1921 taaaaaaaat atatcaggca ctaaaagcct tgttgatgat cctaaatttt ttttagctct
     1981 tgatggaaaa tataatcccg aaagtgagct ggaagctaca atacgctatt tatattcgta
     2041 taatgacaaa gaaaaccctg tgtgccgttt tatggcacgg tatgcatggt taaaaaaaga
     2101 attaggagtt accagcccgt atgaaacata ctgtgaaaaa ttgcaggaga ttatctcttt
     2161 taaagatgat atgtttgttc atcttatttt tcccaccggt tatatcaata gccctgcttc
     2221 aatgtttggc catacattgt taacagtgca atcgcaatac aaccccggat tgctatcctc
     2281 agcagtaaat tatgcagcaa atacaaatga aacatttgga ccttcatttg cattcaaagg
     2341 gatctttgga ttatacaggg gatacttttc tatacaacca tattattcaa aggtacagga
     2401 atatagtgac cttgaaagca gagatatatg ggaatataag cttaatctga atcaggatga
     2461 agtgcgaatg atgctattcc atatatggga gcttgataac atatattcgg attattattt
     2521 ttttgatgaa aattgttcat acaacctgct ctatttgctt gaagtgacac gccctgatac
     2581 gcatcttact gataactttt ttttctgggt tatacctatc gatacactaa ggaaagtaag
     2641 caatgccggc tttattgaaa acaaagcatt caggccttca cgtacaacct tgatacaata
     2701 tatggttggt catctcaata aacaacaact caatgcaatt aaatccattg caaaagggaa
     2761 taacccactt gatgtaatca gtcactattc atttaatgat ttagaaaaag tgcaggtgct
     2821 tgattgtgca tctgaatata tccagtattg ttacgcagat aaacaatacc ctcaaaaaga
     2881 gtatcaaact atatttatta atgtattgaa attgcgaagc accataccca ataccattac
     2941 gtattcaccg ccagttcctc cgcctcctga agatggtcat tactccagcc gaatgagcat
     3001 acagatggga agctttgaaa atgacacctt ttccgaacta tcgtatcgtc ctgcatacca
     3061 tgatttatgt gatgacacat ctggatttgt atctggttct cagatcattt ttggcgagat
     3121 agcagcacgg tattattggg atacacaaaa aatacagttt cagcgatttg acattattga
     3181 cattgaatca attgcaccaa taaattcact tataaaacca ttgagctgga aaattataac
     3241 tggttttgaa cggttaccct ttgagaataa taatgagctt ctggtgtatc agcttaatac
     3301 tggtggcggc tatgcttttt ctttaactca atggttacaa ttatatggga tggcagaatt
     3361 ttcagggctt ttaaataacg attttaaaaa caatattgaa attgctccag gtgcttctat
     3421 aggtttatta ttgcaaccat tcaataaaat gcgattgcgc ttccatgtca gtgattacta
     3481 ttatatattt ggagaatcgt ttaattatgt tgccattaaa acaatagtac agtacaatat
     3541 taatcagcgg tttagttttc gatttgacgt taactattgg catcatgaac aatggtttaa
     3601 tcaattttca ggatcattta tctactattt ctggtgaaac atgccatgaa ctatcgcata
     3661 tacattattc ttttttttag tatattttat tgctcctgcc ttaataaggt tatttattat
     3721 cccgataaca gagttgctat acgtggcaat attcctttcc ccatcgaaga tgtccacatt
     3781 aaggtcaatg atgaagtaac gctgcatggc tggtttgttt tcaataatga tgccgagttt
     3841 actcttatat actttcatgg caacgccggc aatattacca acagagttga aaagataaac
     3901 atattgtaca aaatcccgct ttctgttttt atcattgact atcgtggatt tggagaaagt
     3961 agcgggaaac cctctgagga tggactttat gctgacgcat tggcttctta tgattttgta
     4021 atcaataata aacaactatc gccagatagt atcattattt atggcgagtc attaggagga
     4081 gcagttgcgg catattgtgc aacaaaaaga aatgcagcag gtgttatcct tgattcagcc
     4141 tttactgaca tctattcaat ggtaaaacat cacagtgcat gcttcatgta tatcttcttt
     4201 tcagaaaaat atccgaccat agattatatg caatcaatac gctacccggt gttgatactg
     4261 catagtaaag atgacgaggt tgcccccttt gcaatgggac aggctctgta caatgcaagc
     4321 ttatcaaagc aaaagcaatt tgtaccattg catggctcac ataatgctaa ttttatagtg
     4381 gataaagcaa cctacgttaa tagcatacaa tcatttatac aatcattgaa aatcgcaagc
     4441 aaggagcatg gacaatgaaa aaatggattg cttttactat gttattgatt ttactatctg
     4501 ttaccggtac ctattcagag gattgcaatg gtatatcacc tgcagcatta cgtcttcttg
     4561 ctgaaagtac tattgatcca tgttctatct gccaaaaaaa attacaaagg caggcgtatg
     4621 cgatagtagc cagagaatta tttcccggaa aaaagctaac atttgccagt acctgtccac
     4681 tggcaaaaac taacgattgt caggataatg agctaatcat gcaaaacagt ggatatccgg
     4741 agaaagtagt gataaaagga aaagaagcag caatgccaga ggttttctat aaattttttt
     4801 cacaagacaa gcatatagca ggaatagccc cggatgatta taccagcaat tccatacaaa
     4861 ctatgtacaa aacattaaag ataaattcac ctgttgaagc ccagctggaa ataatttcct
     4921 acaactatag cgactgccca tctgcattat atttacatga taggaatgca ctcatcattt
     4981 tttgcaagat actttctatg aaacaacgac aataatatta ctctattgct tctgctaata
     5041 atgagatggg gtgtactact ttatattttg tattcatctc tatctgccac ttgcatgttt
     5101 cacaatcggt aactacaaaa tccggtttgg cttcttcaat aatcctgaat aactcattcc
     5161 ccactgattg tgaaattcca taatattcat ttttaaaacc ataagtgcct gatatgccac
     5221 aacattctga atgcaatatt tttactgtaa gggcaggaat cttacgtaat agctcaatgg
     5281 taaaggctgc actgcccata cgttcaaggt gacacgggga atggtaggca acagtatacg
     5341 aaagtggttg taagcttaaa atattgccct tttcaaattc ttcaagtata aattgtgtta
     5401 tataagcaat agtattgagt atagggctat tatcaagctc taatagattg ggatactcat
     5461 gctttaacgc taatgtacag cttgatgatg ctgaaacaat ctttgttttt tcatccgaca
     5521 gggcactgga aagcaccttg atattatgcc ttccatgaga ttgcgctttt ttaatataac
     5581 cgttagcaat caatggcact ccgcagcatt tttcctttgt aaggataaca cctttaccca
     5641 gtgcattcaa tacttttacc acatctttgc ctaattgagg atagttataa ttgacatagc
     5701 atccatggaa atataccact ttatctgcat aagtactttg ctgcttcctt acagctttaa
     5761 accatcgtgt aaatgtgccc gaagcatagt tggggaatgt tcgccctgat ggtattttaa
     5821 atatgacatc aaggaaatat tttactatac ctaaatgagt aaaaaaattg acaataacgc
     5881 taaacaaagt tccaaaagtt cccattaaat cagtacggct cagtaaaaaa tcacgtattc
     5941 ttaattcttt ctttgaatat cgccatttag cagtctgaat tatatctgca atctttacat
     6001 ccgaagggca tgcaatctcg caacgtttgc aattagaaca atattttaac gaaatatcca
     6061 gcagttcagg atttttgatg cgcaaccgtt cggtatcagg gcccgactgt tttggtccgg
     6121 gatacagatg agtaacacgc gcaaccgggc agtagattgt gcaaacagta cattttatgc
     6181 aatggtcaaa actaatattt tccagtttaa tcattggtta actcatatgt tttaataaat
     6241 taacgatttg ccctgcagct ttatatcccg tacttatagc aacaccactt ccgcatcctt
     6301 ctttaatggg attgtaatgt gccattatag cgccactgca atacaggttt tgaataatat
     6361 tgttgttgct gtcatacggg tgtaaacttt catcggttag cacaccatac tgtaaaaaac
     6421 catgcgaatc agagtggaag aaattctgat tataccattt attgcgttca ggttcatgat
     6481 gaagttttag cttaaagatg ggttcataca tattgttaaa ttcgctcacc agcccgccac
     6541 taaaaaaact ccctgttgat aaaatataac attgtgcttc cataggattt tccggatgac
     6601 tattggtgta gatagcattg acaacatcat cggtaatatc tgcacgtact actttatcac
     6661 cggcaatgta aacgccgcct aactctgcaa aacgcgattt caatgcattg tcaatacgca
     6721 taccaagaat ggagggaggc agtgttggta cttcatacac cagcaatcgg gttctgcttt
     6781 gtaatttgtt atgaatttga ttaaagttgg ttatacccat aacggctggc attaccacaa
     6841 aatcagcatc atcagcagcc aatcgaatta cgtctgcaat tttttcaata ttatcttctt
     6901 tctcaaaaat tcgtgctatg tctaccgatc taaattcatg aggatgtttc ccgtcatcct
     6961 gaagctgcgg catttctatc tgtttagcta caatgtcaca attagcaaac aatgcatgtt
     7021 ttgaaagttg tgctgctgcc agttgagggt ggaaatctct atacccttca aaattaataa
     7081 taacaatttt tttcatggct ttaaatctac tataaacttc ctcattgaaa acgctgcgct
     7141 gtgaaaaata agttggcttt aatgtaccaa gcgttgtcac atgaaaatga ttaagatttc
     7201 cattgtcaaa aaggaacagc ccctgcagtt cacattcctg tttaaaaaaa agcaatgcct
     7261 caataatcaa gggcttgcca cattttgtat acggatgctg ggcgatagtt cctgttaatt
     7321 ggtctattgc ttcaaatggt ttgtacacca cggtattgtc tctgtatccc agaagatcaa
     7381 ttgaccctga tgaaaaatgc aatgcactca tccccgatga gataatagcc gtatgcaatc
     7441 cttcttttgc acatctgata cctgcagtta atcctgctac accagcgcca ataatgatac
     7501 aatcatatct catgtattat tatcctcaac aagtcctaat ccaaatagtc cctgataaat
     7561 ccagtaggta aattcaaatt ctctgagccc atcgccccac agtaccggtt ttactccctt
     7621 aaacctttcc tcaagaaaat cacgtaaaag ttttgttgat tcgtcaggtg taaagtctcc
     7681 aaactcggta aaaagacctg cggcgcggta tgcacatagc tcaccctgac agggacccat
     7741 gccaatgcga gttcttcgcc tcaaatctac aatatcattc accttgaggt tttttatagc
     7801 atactcaacc tcgccaaggg taaccatctc acattcacat agtaaaccat aattgcgttt
     7861 ttttaaactc aatatattgt gaacgcgctc accatgacgg taatgagtag acccaacaac
     7921 agagttagga atacccgaaa agtattttat ctttttagta taccctaccc ttttttcaga
     7981 gccaggaagc ggtatcttat gggtaaggca tcgcttacta ttgcctaatt tttttgcaat
     8041 cagatcagtt gtcatttcag ccataagcct gtatgtcata agctttccac cagctatggt
     8101 aataagcccc tgcacattat cgcgctcttt atgatcaatt aacactacgc cacggcttat
     8161 atccctccca tccatctcgc ctgacactgc aatgagcggc cgcacaccac aataggctct
     8221 cagcaccctt gccctggaaa cattggggat aagcttctcg ccatcggaca gcaaaacatc
     8281 aatctcatca tcatcaacaa caagctcatc aattgtgtcg tagggaatct ttcttgaagt
     8341 ggttccaata agcgatacgg tatcgccagg tacaatgata tcgccatcag aagggacacg
     8401 acatcgatta actacaacat tatttatgcg atagtcaata ataaccattg aacccttaga
     8461 aggaaacatc ttaagctcaa tgccggctgt tttgcaaata tgctgtcccc atacccccga
     8521 ggcattaaca ataattggag catatatctc cagatactca ccggctgttt tatcataaca
     8581 ctgaacacca ataacctgat tttgcttcag catgatgcgt tcaacttcag tatgagtaca
     8641 aacttttgac cctcgttcaa tggaatcaag aatatttgcc gacgttaaac gaaatggatc
     8701 aatggtaccg tcaggtaccc atacagcaga cagtatgttt ggattgcaat tgggttctat
     8761 tgataatgct tttttaacgc ttatttcttt tgcatctata ccagcaaact tacaggcttc
     8821 taaaagacgg ttatggtagt tggggtcatc ttctggcagg gttataaata aaccaccggt
     8881 aggttcaata caatgccatg caatcttttt taaaatgcgg ttttctttga tacattctgt
     8941 tgctgattct aaatccctga cagcatatcg tgcgcctgaa tgcaaaagtc catgattgcg
     9001 ccctgttgta cccgaagcaa tatcattttt ttctatgagg atagcatcaa tgccacgcag
     9061 tgtacagtca cgtaaaacac cacacccggt tgcacctcca ccaataacta tcacttcagc
     9121 ttctaatgtt ttcactcaat caccttatta tagcatttta ctatggattg tttttcttta
     9181 ccaaagcctt aaaaaagaat tattgaaggt aatgccatac tgaattttac gctgccgaag
     9241 tccctgatta attattatac ttccattaat atattataaa aatatttttt aatattaatg
     9301 cataagaaaa atcattgtga attgatgtat tgattaacat tttgcatttt aaacaatcct
     9361 ttaataaatg tatcaatata tttatcagta agtacatcta tttgtgcttt tgaaagatta
     9421 ggtacattta aaatatcatg ataagcaatc attatgatac ccccaatcaa tatggtggtt
     9481 gttaattgca aatcaacatc atcccttaaa aagccaaaat gttttgctaa acggatatca
     9541 tgcaggatat gcttccgcaa atttttttca aattcgccta ttagtttttg tatgtgagga
     9601 ttaaaaccaa aacctgtcct cagtataatt gatgttagca acctgtcatc atttaaaaac
     9661 attaatgaac gttgtattct gtagtgaaaa taggatattg gatttaattg tttcatttcc
     9721 tcatgactaa tgtttaccgc ctttttccac tcctcaagtg accttattaa aagcgataaa
     9781 aacaaatctt ccttattacg aaaatactca taaatagtac ctttaccaat atgtgctacc
     9841 ttaataacat cttcaacata ggcttcgtag tatccttttt cagcaaatac ctgtttagcg
     9901 caatcaagaa tatgctgcct gcgcaattct gctttcattg caacccctat cttttcttta
     9961 tatatttatt ctgtattgtt taatataatg aaaagttgtt tttattggtc aataactttt
    10021 tatcatttca tttcatttaa tacttatagt gtacaaagcc attttcaaat cctcaaaggt
    10081 aaacggtttt tgcaaaaaat atgaattttc cacattaaaa gcaatatcat tgaatgcatc
    10141 attagaatag cctgaaatga aaattacttt taaattgtgt ataatccgtc ttagcttttc
    10201 atataattca actccattca tttcaggcat tatcacatca gttattaata ttgcaaaatc
    10261 tttatatgca tctactgcat taagggcatc aacagggtca ttaaaagcca taatatcctg
    10321 atctaaaagt aaaagcattc tttgcagcat tcctaatatt gctgtatcat cctcaacgac
    10381 tataatttta ttatgtaata ctgtaattgt ttttgtatta ttttcaatgg cagaaatttc
    10441 aggaatctca ggcatctgtt cagtatattt tgggaagaaa atatttacca cagtcccctg
    10501 cccatattca ctataaactg ttatatgccc actaaactgt ttcataattc catacactgt
    10561 tgaaagtcca agccctgtcc ccttgccaac ctctttagta gtaaaaaatg gttcaaataa
    10621 tctttcaagg gtatccttgt ccatcccact gccatcatct atacattgta tcattgcata
    10681 atcaccaggt ttacatcccg gatgagttgc acaaaatgta tcatctaaaa atttattctg
    10741 agtaactatc tttattgtgc cagtatcttt gatagcatct cgcgagtttg aagctaaatt
    10801 tgttagaatc tgaataaaca tccccgagtc aacccatata tcatatatat cttccgccaa
    10861 ttcaagtcgc aacttgatat tttctccaat catagctgca agcatctttt cacttgtttt
    10921 aattatttca tttggattta gtcttacagg attaaccttt tgttgacggg taaatgttaa
    10981 aagttgcttt actatatcag cgctacgcct tgctgcatga gctatctctt ctatatcggg
    11041 ataaataata ttatccctat ctattttaaa ttttattaat gcagcatatc ctgatataat
    11101 gcttaaaata ttattaaaat catgcgctat accgccaacc agcctgccta tcgcttctaa
    11161 tttttgtgcc tgacgcaact gctcttccaa tttttccttt tgtttttcag catctatttg
    11221 ttgtgataga tcctcaacaa ttgccagacc gccaacaacc gtatcttcca caataagagg
    11281 tgaagacaaa acacgcacgg gcgttacttt attggcagtc actgatttat accagtccct
    11341 gtattcggct tgttcaccct gcaaacagcg tttggttgct ttaacaatct tttcatcggg
    11401 cagttttagt gtatcaagcc ctatcaaagc ctctcgtgtt gaacctataa ttgtaataaa
    11461 attatcatta caagctgtta taacaccata ggcatcaaat gcatatatac caacaggtgc
    11521 attctcaaaa ataagtctga atagctcttc actcttcttt aatgataact cggcctgccg
    11581 ctttgctgta acatcattaa caatagaaaa aagatactgt ttacctttaa ttgtgattgc
    11641 actgctatat acatcaacat ctatgatgtt accatccttc tttctatgct taaatgtaaa
    11701 atacacgcgt ttacatgtcc ttgcttcttc catacgtgct tttatctcat caggtggaag
    11761 ggtattaatc tgatgtatat tcattgattg caattcatcc tttgtccatc cataatactg
    11821 gactgcaata tcattggcat caactatacg tccgtcagca gggtctataa tcaacatcat
    11881 tgctttgctg tttttaaata tggttctata tcgctcctca ctttcttcaa gttcaattgc
    11941 tatctgccgg cgttgagtaa tatcactcaa agaaccaaat agagcgctaa ttatgccatc
    12001 atcatagcga acagcatgca ggtgtatatc cacccattta attgctttcg atttatcata
    12061 aatcctgcat tcagcagaag caaaattctc aattgttaaa gtatcaaaca acgaataata
    12121 ttgtgaaata tcatcttcat ggattgactc ctcaaaccag ttggcatgca tcatttcatc
    12181 aggcgtatac cctaatacta tctccacatt ggggcttgcc cagctaatgg tattatcaat
    12241 cggattcata atgaatgcca ccgatgggct tacactcaat acatcctgca gcaaatcagc
    12301 acctgcctgc aaatgttgca gggctaatgt ccgtttttcc acagtagtaa aaaccagagc
    12361 tattgctatt aaacctgatg acagcaccag gatgtagggt actcctattg tggtaatgac
    12421 tgtcaatcct tttggccatg gtaacagcaa ctgacatgat agtaccataa catgcgtgac
    12481 aactgcaaat atgataatcc ccaataatgg tatttcagat ggttcatttt tatatatcct
    12541 gcgcattgct aaacctgaca atgccgaaac tataatgctg gcaacacctg catatactcc
    12601 tgcgccgccc agatatatcc tgtaaaaaac agcaacaccc gatgcaacaa acgaggcaac
    12661 accaccgcca aacaatccgg ctaatgcaat aacaaccgaa cgaccatcat agatgatgtc
    12721 ctcttcaaaa tgaaatggtg tcatcatagc tgcaattgct gaaaatccaa acaacaatcc
    12781 tgacagtatg cgataggtat tgggataatt ctttcgtaag gtaaggattg ggctccatat
    12841 cacactgaga gcaacaagaa gcgttatatt gtgaatcaat gttaaaatca tacgttaaac
    12901 ctgctatatg taggcataca tgaaacagaa atgatttatt atttaattat actataaaaa
    12961 aataaaaaat acaactatat ttttattgta tcaggttatg gagcaagcag tagtttttta
    13021 aattatgaaa aatctttttt aaaagttcat atccctgtaa tgcctgcata tcaggatacc
    13081 ctcctttgtt gcaacaacat caattgatcg ttcactacct cttttaataa ccacatctat
    13141 tttctgattg cctattcttt tttttaatgc aaccaaaaat ttaaacttct tctgggttaa
    13201 tgaatcagta ttacctggta caatatataa atcaatatct cccccacgca tggtatcgtc
    13261 tgttctactt ccaaacagat atatttcgcc ctcatcaaaa acttctatga aactctgttt
    13321 tatacattca atatcagact gctttaatcg cataatacta cacccactat actgtactca
    13381 ctataatttt ctcatcctgc aaagttgaca tagcatagcg tataagccca tgcaactata
    13441 gtcaagtata atacatcaca acgcaacagc atacacttat actcttttta aaaaacatca
    13501 taaacgtatg attaataatt ctgcaatggc aaaaatggta ttatgggaat aaacacattt
    13561 cataagccta aacttgcacg gggtactgtc ttttctactg ttccaaatgg atcgttaaac
    13621 tcctgctgct tttgaaacat aagtttatgc tgtcctctgt tgttaaggta ttttgctaat
    13681 tcaaggttga tactatttcc aacatcaaca tttttctttg ccagtaactg tcgcaaatac
    13741 ccttccgcct tcgttgaaaa accaatagag tcgcccagta tccttcccgc ttcttccgga
    13801 ttatgaaatc caatagagtt ttccgcaccc ataaagataa gcctgtatag cgcttccagg
    13861 taataatctt tagcagttat atacagagga tcattttgca aaccatcctt aaactgtgta
    13921 tgaatttttt caaatagctt tgccaccacc gcttcatgat atcctgcttt caaaagcaga
    13981 gacagtgttc tgtcctgaat ctctttgatc tgttttacaa ggcgctcatt tgactgcgga
    14041 tgacacttta agcaggcaaa tagatcatcc ttcagcgggc tcatcagatt gtgatctgaa
    14101 attttaaacg atccaacccg tttatacggc atgtggcaat cagcacaggc aagtccggcg
    14161 ttgaaatgta cgctttgtcg tgtaaaaaat tcaaattccg gatgacgaag aaatccaacc
    14221 ttaaaaccgg tgacctcctg tttccactcg ccccaatgag tattcttctt tataagcttg
    14281 atgatattct caattgaaat attgccccac ctgctgccgt cataagggtg ttccagccca
    14341 attgattgca tcgcttcatt tttaggtata ttataggtaa catgacactg agcgcaaacc
    14401 agcatccgca tctcctgccg tgaaaattca tcctttccca attttgcaag tgctcgctta
    14461 aatatttctt tgtttaactt caatgacatc gttttattgt catggcaatc aatacaactg
    14521 gctccaagat ttttatgcct taccggtatt ttagcaatag catcagccca tggcatgcta
    14581 aaaaatgaat tgccatactg cttaaccaga gcatccatgt acggtgattt acaatttaaa
    14641 catacccctc cagcctttac ccgtgaagaa tcaatttcat cctgatcaat gcgcatatac
    14701 cagtgcccac gcggctcatt atacgctata ccaaaacccc accctttaaa gagcaatgcc
    14761 atgaatggat attctgaaag cttatcatac acaactttat cagtgtccca tcctttttta
    14821 taaaaacttt ttccggttgg tcttggctct tttgtctttt gccatgactc atattctaac
    14881 ggataaacct tgccccattc agcagggtca tactcattat caggaatagc tccaacaagc
    14941 acaagctttg gttcggttgg cccaagcact ccaataagat acatcaccaa tgataccgct
    15001 atcacaatac ctattgcagc aatgatgtgc tttcgctcaa ttttttgaat caacatagtt
    15061 aatttttcca tggcttacct cttatgttcg atagttacta atgaataata gaatgataaa
    15121 aatagcgatg gcaatcccag cattgcatgg caccgttgtc aattcttgat accatctcac
    15181 agtggcagcg aatacaatta gcctgaattg ttttctttgc atgtgatgaa gaatgaataa
    15241 tttccggata taatcgtgta taatacatca caacatcctt aacaccatcc agccctttcc
    15301 ataacagata attggcatca ttgttatggg gcagatgaca atccacacat gtaatctgtg
    15361 tatgtttgcc aatataaaac caatcttcat attggggaat catcacatga cagcttccac
    15421 aatactgtgg cattgaagtt ttttgcataa attttgcagt accataaaca gcacatacta
    15481 ccattacaaa caataaacca aacacgctat atagtttata tttcatggct attccttaat
    15541 cataaccagc agcatctttg ccttttcaat agctataagt gcatggggta cattagcagg
    15601 cataataata gcctccccga cagcaaccgt atgtccctgc cccccaatgg taatcgtaaa
    15661 ctttccgtcc agcaccataa caaacgcatc aaaaggtgca gtatgctcac ttaggctctg
    15721 agccttatca aaagcaaaaa gtgtaatagt accaccacta ttttgaataa gggttttgct
    15781 tacaatagca ctgttttgat agtcaataaa attaacaaga acacatgaat ttttcaaagc
    15841 ttcatctatc atggctacct ctcctgaaat agatttatgc gccattgtca tgctaaactc
    15901 gtttcagcat ccctttaaca cagctttata tcacattaat atcccgcaca atcaatccag
    15961 caatctcttt tgccccgtta tcttctgaat atcagtaata ttctgggtta cctccagaca
    16021 tcccatatac tcaccatttt tacgcactgc ataataggta atataaataa ggttaccatt
    16081 gagcgtaatc cagaattctg ctttgtcacg tgtgccctgt ttcatctctt caagcagttt
    16141 gtttacaacg tgaatacttt tatcagggtg gcaattctgc acagtcctgc caataactgc
    16201 ttttgagcgc acaaagatac gatcaggtgc attgttaaaa taacacacta catcgttttt
    16261 gtcaataaat gtaaaatcaa ccggcatcgt atcaagcata ccggttaact gctcaagcgt
    16321 aagtttcccc gtttcaagtt caatgctgcc agcttccatt cttgttgtca ttcccccacg
    16381 tttacttata tctgcaacag ggctaaacgt agtatatcca attgcatcaa actcctgttt
    16441 cacaagttcc cagtcatcat cattaaagaa tttatatgat gttggaaaca ggatattatt
    16501 ttccttgtaa aagtggtcga ctaatgtttc agaaagctgt attgaaagca tttgtaattt
    16561 ttgcgatagt tccatggtaa cattctttgc caataaatca gccgtttcat atatattatt
    16621 ttcaattgcc ctgatggtat catgttccat ccacataatc ttaggtggct gagttacacc
    16681 atatttttcc agaacaggga atataacatt ctcctcacga agataatgca ctgatgattc
    16741 cttaaaaaaa ctcaccagtt gctgtaaccg cgcaaatacg tcaggcgttg gagcatcaat
    16801 aagttttgtt gcactttcaa ccagattctg cgcaaattca agcaacctgc tatgttcagt
    16861 atacagaata tataaagggt gtcccagtgg cacatcaacc cttccggcat caattgattc
    16921 cctgaataaa tctatgtgaa gctgacacaa tctgtgtact tcctctgctg gcataccttc
    16981 cttaatcagt tcctcttcaa gcatactgat ctgatgcggc ataacattgc caaattcttt
    17041 tttaaacagt atcttcatct gttcaatatc tgcaccatca tgaagcattt ttatcatctt
    17101 tttaagctgt tccttctttt gtcccatatc acttaaaaat tcgctcatta catcctccat
    17161 tcataaaaat tttttaatat atgaaatact tcctctgaat atcatgcgta tacctgagta
    17221 tcgcattact tcacgtattg tgcttctttt aggttctgca taacaatgtg tggtgcaatc
    17281 tttacaggaa ggttttggat tgtatgggca tagtatacgc ttgctcgcgg catacagcaa
    17341 caattttgta cactcatcac ataattgcac ttcaagagga ttaacatata acgccacttt
    17401 cccatgtcca tgaaccaacg attttgctct tgcagggtgt tttccattgc agtatatatt
    17461 aatgaatttt cccagcacac caatgtcatg ctttattgca ttctggttgt tagcatccat
    17521 agctttcctt atataatttt cttatcagta aagttacagc agccaatgtt aataccgtca
    17581 tactgaaatg tgcgttttgt aaatgaacac ataaaagtat tgcttgatag ctgtttgcaa
    17641 tgctcgcaat taatacataa cgctatataa tcaatagctc catcatcctg cattgccatt
    17701 accatacggg caagcacgcc aaatgctatc cttttatcgt ttttatcaat ttgtgataaa
    17761 tagcgttgca ttgtatgtgc agtttgttca atgtgcgtta taataaagcc tgcttttttt
    17821 gttaatgaca cataaaagct gcgattatca ttgctattga tatttttttc tatcattccc
    17881 tttttcaata aggttttgac tgcactgctt gctgttgctt tgctcacata caattctttg
    17941 acaatatctg ttggattaat ctttttatat tttgcacatc gtttgattaa tagcaatatc
    18001 tgaatttgca atggagttaa gccatatgtt tcagatattt cccagagcat ggatctttta
    18061 atttcagtaa ttttatcaag aatggctatt aagttttcat ctagatgtat tgtatccatt
    18121 atgtactgtc ctgtaatata gttagtagta ctaactatat tacagacgta gaaaacgtca
    18181 aattattttt aataaaataa taattttatt gcattgagat tataattttt tcattgctgt
    18241 agcagttagt atggcttgcc ggtataatgt tttatgtgta aaagatttat gcccattatt
    18301 ctttattctt tattctttat tctttattct tttccagtat ctcctgtgcc agcattttta
    18361 gctttggaga taccttaagc ggaggaagtt ttacacggta tggactttcc tttactgtat
    18421 cgttccccaa ccatttacca agcgcaaata ccatgcgttc cattatttca tcgatcttct
    18481 ttatactgcc gctcaatcta tagaacttaa gagcaataag tggcaaccgt ttgtcataat
    18541 agtaataatc gcctatccgg tgcaatccat ccttatatcc tgtctttaca aacagctcaa
    18601 tcgcttcttt tatctttccc tgattaaagt attcatttcc tttacggatt aaagcaattt
    18661 tttctttatt gtccatagtt catcccttac tatataaaaa tatcattatc agtaactatc
    18721 gcccatgaaa gtatttttaa ttattacaaa tttgttcaca tcacaatttt gtacacacct
    18781 accacttttt attgacagat atcccctata gcgatagatt tgataattat taaatattaa
    18841 tcagctttta tgcttgcgtt tatactatta tcgatcattt tgcaagcatt tatttataat
    18901 atttattgac agttaacgtt aactggtagt tcaatagtta tcaacatttt ttaccagaga
    18961 gatgattcca tgggacatat agtcatcgat agtaaccgtt gcaagggatg ttatttttgt
    19021 ataacatact gtcccaaaca aatacttgtg atttccaaaa atcacaacat acagggatat
    19081 tttcctgctg agcttgctga tgataaaaaa gaacactgca cagcctgtaa gatatgcgca
    19141 ttaatgtgtc ctgatacagc aattgaggta ttcaaatgag agtattaacc aaagggaata
    19201 tagcgttagc tgaaggagca atacaggctg gttgcagatt ttattttggc tatcccataa
    19261 cgcctcaaaa tgatattccc gaataccttg caaaaaatct ccctaaggtt ggcggcacct
    19321 tcatacctgc tgaaagcgag attgcttcaa ttaacatgat actgggtgct tcagcatcag
    19381 gcgcccgagc aatgacatca tcttccggac ctggtatatc gcttatgcag gaaggaattt
    19441 catatttagc agggtcagag cttcccgctg tgattgttaa cattatgcgt attggtcccg
    19501 ggcttggcgg tatagcacca acacagggtg actacaacca ggcagttcat ggaggcggtc
    19561 acggtgacta ccgtaatata gtgttagccc ccgcctcatg tcaggatatg tttgaccttg
    19621 ccataaaagc atttgaatta gccgataaat accgtaatcc cacaatgatt ttagcggatg
    19681 ccatggtagg acagtttcag gaagcatgcc gtcttacacc ggaaaaacct gttatcatcc
    19741 ctgataaacc atgggctctt accggtgcac agggaagacc tgctcaattc attaaatcat
    19801 tgtttttagg tcccggtgag atggagatac acaatcttga tttaaagaaa aaatacgaca
    19861 ccatacagca aaatgaagta ctgtatgaaa cgcgccattt agatgatgca gacattatga
    19921 ttattgcatt tggaacacca tcacgcattg caaaaacagc tatacatctt ttgcgcgaag
    19981 aaggcataaa agccggctta ctacgtccgg taaccctttt cccatttcca gctaatgcta
    20041 tcaatgaata ttcaaaacgc attaaaaaag tgctggttgt tgaaatgaac accggacaga
    20101 tgctcaggga tgtacaacta tcatgtactg ccgataccgc tgtctatttt tatggaaggc
    20161 ccggtggtgg cataccattt ccggatgata ttatgcgtga agttaaaaaa attatgtaag
    20221 aggttaccat gcaggcagta tttacacgac caaaaacatt aaaagatacg ccaacccatt
    20281 tttgtccggg ttgtactcat ggtatagctc atcgcatagt agcagaggtt attgatgaat
    20341 taggtattgg cgaaaagacc attgccattc ccggtgtggg gtgttgtgtt tttttatata
    20401 actatcttga tattgatgtt cttgaagcgc cgcatggaag agcacctgca tgtgcaacgg
    20461 gcatgaaacg ggcaaatccc ggccgaatca tttttgccta tcagggtgac ggcgatatgg
    20521 cagctattgg cacatcagag gtaatacatg ctgcaaatcg cggtgaaaat attactgttt
    20581 tgtacattaa caataccgtt tatggaatga caggtggaca gatggcgccc accacgcttt
    20641 taggtcagca tacaacaaca accccatatg gtcgtaactt tcgcaatgaa gggtatccta
    20701 ttcgtatggc agagatgctg gctacattgg aaggtgttgc ctattccact cgcgttgccc
    20761 tccataaacc aaaatatgtc atgcaggcaa aaaaagctgt ggcaaaagca ttcaccatgc
    20821 aaataaacga gatggggttt tcaatggtgg agatgctttc aacatgtaat actaactggc
    20881 gggtttctcc aactgaagct attgacaaaa tagaaaatga aatgataccc tatttcccgc
    20941 ttggtgtttt taaggaaagg acaggcaaag actttttata gtggcgatag ttactgtata
    21001 gaatactgtt gcaccataag gcattttttt ggatgaagtt cacactgttt gttttttatc
    21061 gccgacaatg agatgaacat gcatcattgc cgttattctg agacgaagaa tctcgttgca
    21121 gaacgtcatt ctgaacaagg tgaaaaatct cgtcatctta cgcaagaaga tattcctggt
    21181 gactgcgcct gcagaatgac aatggagtac atatcattat gaaccacgat tcaggatcaa
    21241 aagttatata tttgattccg aaaaagctcc agaatgacag aagcgtatag tacctatttt
    21301 tacaggacaa aacctcctat ccttacaaag gagattgtcc gtgagtgtgg ttaataattc
    21361 aattttcaga ggtattcaca atgtactatg acataataat ggcaggattt ggcggacagg
    21421 gaatacagat tataagccag cttgttgccg ttgcagccgt caataaagga ttggaagtaa
    21481 cctatctacc atcgtatggt gtggaaaaac gtggcggcag aaccaatgtt tttttagtta
    21541 tctcagacag agagattggc tcacctgtta ccaaccaccc gcgcgcactt cttgtcatgg
    21601 accagaattc ttttgatacc tatattccca tgttacgccc tgatggatta cttgttgcaa
    21661 atggaagcct tatcaataca tcaagttaca aacgggatga tattgtatca tgctgtattt
    21721 tttgcaatga agaagcaata aaattaggaa atcccaaact tgccaatatg attgcactgg
    21781 gcgccttttt aaaacatata aattttttaa ctattgatga cattgggaac ataatgaatg
    21841 aagttatacc ggaaagatta tcgcatacca taggccccaa catgcaggca ctacattatg
    21901 gtaatactat agcttaatga cgctgtttct ttcgctcaag cttatttcta tacattgttt
    21961 tatcagctct taccagaata tcttctatat taccggaact gtcatgtatg aatatacttc
    22021 ctatactcaa tgatagctga tattcccgta aactttttgc attaaattca ttaatatttt
    22081 ctataagtcg gttctgcata atttcaatat catgtctatt acaatcaatt gccagtattg
    22141 caaactcatc accacccatc ctgccaataa tatcagaatc cctgaaacac agtcgtaaaa
    22201 tagctgcagc atcaattaac gctttatcgc cttcatgatg tccaaacgta tcattgatga
    22261 gcttcatatt atccatatca acataaaaaa ttgccatccc cagctcactg cgtctattta
    22321 ttttcatctg ctgctcggct aacatcataa agcctcgcct gttatacaat cccgttaatt
    22381 catccttaat tgccatctcc cgtaaatttt cacgcatggc aataattgga gttacatcag
    22441 ttgcaatgca cactatcctt tccaattttc cagctttatt ataaatattg gaggtagtaa
    22501 tggtaagata ggtgtcttcc ccctctttat ttattatgtg tatctcaaag ttacttacat
    22561 caccctgttg tatttgctca ataaattgcc gtatttttct tttatcctcc tgcgggtgaa
    22621 tcatacttct aaaccaatgt gttcctttta aatctgatag tgtaaatcct gtaatttttt
    22681 caaagtaatc attaataaaa ataatatcgc cctgaatatt catttcaata atagcagcgg
    22741 gtattctctg tactaaattt cgatatcgct ttcccgactc aattaatgca agttctgcat
    22801 ttttacgctg tgtaacatcc cttataacag ccagcacacg gttttttata accggcacaa
    22861 gccttgcctc gcgccaccgt gattcatcac caatttgtat tgtatattca atgctttgta
    22921 tggtatttgt agacatggca tcatctacag cacttgcata aagtgttgcg atatgcgttg
    22981 gcatcacatc ataaaaggtt ttcccaagcg tttcttctct cgtagcatat agtattttct
    23041 catcaccata ctggtaatca acaatatagc catctttatc agctattaca taaatatcag
    23101 ggaaggcttt cactattgct ttcaagtttg tgctactctt taataactca atctccatct
    23161 gttttttttg tgtaatatct tcacctgagc tcattgaagc gagtatagta ccatttgcat
    23221 cgtatatgat tgtattatgc catgcaatga gttttaattc cccactttta gtaactatag
    23281 ggttttcata aaattcgaca ggttttatat tccctttcat tagttcaata aaagtttttt
    23341 tcacatcaat agccacatcg tgtggaaggc atgtatcaaa ccagtcttta cccaataatt
    23401 cttcagaagt ataacctaat atttcacatg ccttgctatt taccagtgta atcttccctt
    23461 caggattaac cagcacaatc ataaatggtg aaatgtccag atatttctgg aatgtatcac
    23521 gctgttgctg tataatttcc tgatatacct tatggtcagt aatatctgta aaaatgataa
    23581 cacaaaattt aatcccattc aatgtatacc caaaaaaatt gaattgaaac cagcgtgtat
    23641 tttcgttgtg ataaatttta ctttcaaaag aaatcaatga ttgtctgccc gatagtattg
    23701 ctctaatagc atcagaaaaa cgtgatttag tttcatcata taagcaaccc cccttatcac
    23761 ataattcaat gatatcatgg tgtaaactat ctcccggtat ttccagtact tcattaacaa
    23821 aacgcttcca ttcgctgttt gccattataa tgctataatt atgatcaacg ataactatct
    23881 gccttggaat tgtatcaagg atagtttgca ccaacatttt actttttctt aacaattcac
    23941 catcagaaac atcactatac aggatgacat gctcagaatc aaatggcacg catatgccat
    24001 gaatcagtat attattattt ttaagcgata gttccttaat attaattttc cccttgatag
    24061 aaaatgcact gaccattgca tcaaacagta cttcatgatt agaacatatt tcttctattg
    24121 aagcattttt gattttatct tcattaaatg aataattttg acaaaagcta ttattggcaa
    24181 aaacaattgt accatttcta ttaataataa tagaaggttg ctgtatacaa tctaaaaatg
    24241 aaaagttaat atttcttttt tcatccataa aaattatgct gtactatttt agttgttact
    24301 ataaaaatat ttaacgcata tcataaaact tacaatttgt caaatataga tgctattttt
    24361 tattttaaaa atagcatcta ttattataca gcttttttac ggtatagact ataatttatt
    24421 aagatttcga tacaatccca ttcaataaat ttaataaaaa aagcgcatca ttacgtgatg
    24481 cgctttatga aacatttgta aactataaaa attcattatt ctaatggtgc accggtaaaa
    24541 atatgttcac tagccttaac aactgctgcc tcaccatcta tgatatagtc aaccatgcca
    24601 aagaatttct taaactcagc cccaagataa aattgcgatg ataatacctt cccgttgtaa
    24661 tatgcagctt cactattgtc cgcaagcaat gcattaagat catctccctt cttatcgcca
    24721 attagctctt tcatctttgg ctgtgtcagg cttagcatcc ataaatgcat ccatgccaat
    24781 acaaacattg acatagcctg ctgtaatgga gttgcactgg caaaaatgtg taaaaatttt
    24841 cctgcagcca tctgatcttt aaggtaactt attactacat caactttttc taatcctttt
    24901 tgtactacat caacatattt gtcatcaaca acgcctttag ccctatcgat agtttcctga
    24961 actcgtttct tgaaaacact ataattatac tgttctggat tcatcaatat ttttcgcatg
    25021 gtaaggtcca tagactgtat accattagta ccttcataaa tagaaagtat tttagaatct
    25081 cttgcaaatt gttcaacagg ataatcagaa caaaatccat acccgccata aacctgaata
    25141 gcttctgatg ttatcttcca tgcattatca gtattaccag ccttggtgat tggaattaaa
    25201 atttcaatca tcgcctgcgc ttctttcttt gcctcaccat caagagcatg tgcaagatct
    25261 tcctgataac caacataata tgtaagaagt ctcatacctt caacatatga tttcatccat
    25321 aacaacatcc gcttaacatc aggatgctgt ataatactaa cttttggtgc atcaggattg
    25381 agcatctggg atacatgaac accctgcact cgattacgtg cataggtaat agcatgcata
    25441 taagcagcac ttgaaacagc caatccctgt aatccaacat ataaacgagc ttcattcatc
    25501 atctggaaca tgatcttcat accctgacgt tctttgccca acaaccatcc aatacaatcg
    25561 ccagtatctc caaaactaag tgaacatgta gctgaagctt taatgcccat cttatgttca
    25621 atgccactac aaacaacatc attgaattcc aatgaaccat cagctttcac cctgtatttt
    25681 ggtactataa aaattgagat tccctttgtt cctggtggat ctccttcaat tcgtgccaat
    25741 acgggatgaa taatattctc tggcatgtca taatcaccac cacttataaa tatcttctgc
    25801 ccaataattt tatatgttcc atcatccagt ttaattgctc ttgttttaag attacccaca
    25861 tcactacctg catcaggttc ggttaaacac atggtaccgg tccactgacc tgttatcatt
    25921 ttaggaatat agagatcttt catttcctgt gtaccaaaat ttctaataag gttcaacgcg
    25981 ccaattgtta aagccggata cattgaaaat gcaacactac ctgcagtaaa aaattctgtg
    26041 cagcattgcc caattgcctg aggcattccc atacccccaa tttcagggtc attggcaagc
    26101 ccaataaatc ctgcatcata catagcctgc aatgctggtt tataacattc aggtaaaata
    26161 accttcttgg tatcaggttc atattgaaca ccaattttat ccccttccat atttgcagga
    26221 tatacctgct caactgcaat agtctctgcc agattcagta catcttcaaa tgtatcttta
    26281 tcaaaatcag caaacatagg atattgtgta agtttatcaa cacctaatac ttcaaataaa
    26341 acaaatcgcg tatcccttgt atctaccaaa ggattcagcg acattactcc agttgccata
    26401 ataagacctc ctgtattaca attttaatat atgtatgatg gtattatgtt aaataccata
    26461 atagctgtat tcacgatatt ttttttattt attttgtcaa ttatttgtta acatataatt
    26521 tatgttattt actgtttatt tttgtatggc atgtttaaac atattataaa aaaaagacag
    26581 gtattgatta cctgtctttt tttttaaaat ttgttttaaa gcatctctta ttcgttaagc
    26641 atccctgtaa atatttcttc acttgcctta ataacagcag attcaccacc aagcaaggct
    26701 tcaattttcc caaaatattt agggaattct gcacccaaat aatattgaga tgataatact
    26761 ttcccactat agaatgctgc atctgcatta tctttcaata aagctgctct ttcttcacct
    26821 tttttatcac caaccagctc tttcattttt ggcaatgtga ttgttaaact ccacaggtgc
    26881 atccatgcta ttagtaacat atacatcgcc tgctgtaatg gtgttgcatt agcaaacaaa
    26941 tggaggaatt gaccagcagc catctgctgc ttcatcattt ctatcacttt atctaattcc
    27001 tgaacaccac gctcaactaa tgcaatatat ttatcttcaa caataccttt ggctttatta
    27061 atagtatcag caatacgttt cttcaatatt gtatagttat actgttcagg attcatcaat
    27121 atcttacgca tggcaaggtc catggactgt atgccattag taccctcata aatagcagta
    27181 atttttgaat ttcgcatcat ctgttcaaca ggataatcag caacaaaacc atatccacca
    27241 taacactgca tagcctctga tgtgataagt actgacatat ccgagcatcc agctttttca
    27301 attggagtaa taatttcaac caatccctgc gcttccttcg ctgcatctcc ttctaataat
    27361 tgtgcaagtt cataattcag atacaggtaa taaatcattg cacgttcccc ttccacatat
    27421 gacttcatcc acagcaacat tcttttaaca tcagggtgat tgataatagt tacacccttt
    27481 gcatcaggat tcagcatctg ggttacatgg gcaccctgta ctctattttt ggaataggtt
    27541 gtagcatgca tatatgccgc tgatgaagtg gaatgagcct gaagaccaac gcccatacga
    27601 gcatagttca tcatcttgaa catgatcttc ataccttttc tttcttcacc caataaccag
    27661 ccaatacagt tgttattatc gccaaagctc aatgtacatg tagctgaacc tttaatgccc
    27721 atcttgtgct ctatacccgc acagttcaca tcattaaact cacctaatga gccgtcagca
    27781 tttaccctgt atttgggaac aataaagata gaaattcctt tagttccggg tggatcccct
    27841 tcaattcgtg ccagtacagg atgtatcata ttttcataat agtcattgtc acctgaagat
    27901 ataaatattt tttgaccaga aattttgtat gtaccgtcag attggcgaac agcttttgtc
    27961 ttcaaagcac caacatcgct accagcatca ggctcggtta aacacatagt tccaccccat
    28021 ttccctgagt acatatttgc cagatacata tgtttctgtt cttctgtacc aaatgcttct
    28081 ataagttctg ctgcaccatg tgttaaaccc gggtacatta aaagtgggta gttagctgca
    28141 caaaaatatt cattacatgc ccatgcaata acctgaggta ttcccatacc accccactca
    28201 tcatcaatat ccatcccacc aaaaccagct tcataaaaag catctaatgc tggatgataa
    28261 cactttggaa tagtgacctt tttagttgct ccatcataat tgcaaccttc tttgtctccc
    28321 tccgcaaatg taggatatat ttgctcaaca gccatagttt ctgcaaggtt tatcatgtcc
    28381 tcaaacatgt ccttatcaaa tgcagaatac tttggaagct tgcaaagctg tatgacatcc
    28441 atcatttcat agagaacaaa gcgtacatcc cttgaatcaa ctagtggatt taacgccata
    28501 taaaacctcc tgattttaaa ttataacgca tgttatatta ttacatttta acgtgtatgt
    28561 ttaatttata aaacacaaac cataacacaa ggtaacgtta taatatgata tttttctgtc
    28621 aataatgaaa aatatttttt tgtataattt atttcaaaca tacgaaaaat atattttatc
    28681 gtattaaaac tgatatgcaa taatgctctg accccttttt tacacccact gtgcaatgga
    28741 gtagcgcttg cgatacgcct cctcatactg caaaaaacgt ctcacccgtt cactgaaact
    28801 gtcccccagt tgcagaaagt actcatgatg gtctatcggt acaccaacat cagcaccctc
    28861 ctgta
//
LOCUS       DAOOJH010000023        28728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1727_length_28728_cov_8.576152, whole genome shotgun sequence.
ACCESSION   DAOOJH010000023 DAOOJH010000000
VERSION     DAOOJH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28728
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1727_length_28728_cov_8.576152"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="PLJ75_04980"
     CDS             <1..1594
                     /locus_tag="PLJ75_04980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD04603.1"
                     /translation="ISLLYLSFLQKKEVPPMKLFILIFYGLILLLLSIFGAHRYYTMY
                     LFIKHSRNKVKPKAVFKNLPKVTVQLPIYNEQYVVERLINAVTQLEYPRKKLEIQVLD
                     DSTDETSALAEKICKQKRKEGYTIIYIHRTDRTGFKAGALENGLKYTDSEFIAVFDAD
                     FMPNPDFLQKTIHYFTDPTIGMVQTRWDHINRHYSLLTECQSILLDGHFMIEHTARNR
                     SGRFFNFNGTAGVWRRQAIIDGGGWQHDTITEDLDLSYRSQISGWKFLYLPDVTVPAE
                     LPIEISAFKAQQFRWAKGSIQVFKKLYKTILNAQLPLKIKIESFMHLGANFAYLLMVF
                     LTLLMPVALWVRYYGGFKKFAYLDLPIFILATLSVIIFYFYTELSILYHNKNSFDSKF
                     KPLVYLPMVLAVGIGLSINNSKAVIEALFNKKSDFNRTPKYNVASLHRINAVEHVKTL
                     LKNKYSLHTINIVSIIEFILGLYMSLAVYFAFDKNLYVSIPLIMLFQVGYLYTSLFTI
                     INSITHHIIVIANSLRRGVVND"
     gene            1738..2157
                     /gene="nikR"
                     /locus_tag="PLJ75_04985"
     CDS             1738..2157
                     /gene="nikR"
                     /locus_tag="PLJ75_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPD04604.1"
                     /translation="MNSVIRFGVSIDEKLLDRFDALINEKGYVNRSEAIRDLIRDMLV
                     KEDVSDPNSEVIGTLTLVYSHDVREIADKLNDLQHDNYKYIISSVHVHMDEHNCLEVL
                     LMRGTAQQVKKIADNLLAIKNVRHGKLTVTTTVDYHE"
     gene            2265..>2614
                     /locus_tag="PLJ75_04990"
     CDS             2265..>2614
                     /locus_tag="PLJ75_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04605.1"
                     /translation="MYSWMSAFIITLLLLFSISCVPPAYRSKPDTSRDKHNYYKFEQP
                     EEYTPFQDDTIYIQKNKNDTTMSVCYDKYSKYEEDGTKHIMSDSYQHINPAPENNSKS
                     GIWHTVKKGETLYRL"
     assembly_gap    2615..2714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2715..2918)
                     /locus_tag="PLJ75_04995"
     CDS             complement(2715..2918)
                     /locus_tag="PLJ75_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPD04606.1"
                     /translation="MECKKDRNLKVCTCTYEPCSRKGLCCDCISYHLKSRELPACAFS
                     KEAEKTYDRSFEHFVKLVSAGKI"
     gene            complement(2932..3471)
                     /locus_tag="PLJ75_05000"
     CDS             complement(2932..3471)
                     /locus_tag="PLJ75_05000"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPD04607.1"
                     /translation="MNEFKPLPIQDSHRLINAGNVILISAQSKEKKTITTVAWHMPVS
                     NNPKLLSCALANKRYSLELIKESKSFCINIPDFAMLDTVIFCGTYSGRTVDKCKEAHL
                     TALPCSDIDGFFIAECVAYIECQLYSMVEAGDHTIVIGQVTCAHAKIHCFTPDGVIDI
                     NKVQLIHHLGGTHFGILKN"
     gene            complement(3464..4387)
                     /locus_tag="PLJ75_05005"
     CDS             complement(3464..4387)
                     /locus_tag="PLJ75_05005"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPD04608.1"
                     /translation="MWLLSLIDAAQNEIKEIQNNTKCILHVLSEEIGERTLRKYDNLN
                     KSRQFIYNYLHQSNDNVYEEKYLVDNLEVSNIIAEIPGFEHSNEIIIIGAHYDTIEDT
                     SGADDNGSAVAGLLELHRMLSQYTFRKTLRFVAFTLEEPPFFSGPYMGSMVHALGCKD
                     KGENINLMICLEMIGYAGNRMQQDYPLRSMQNEYPPYGNFLAVVSLPSYAPYAYKWKK
                     EYNKYAKNKIVDMIGPASIPGINLSDHYSFNKFGFPAIMITDTAFYRNKNYHTEGDTY
                     GTINFKFLSESIFNTFMAVKTLSNERDILDE"
     gene            5109..5972
                     /locus_tag="PLJ75_05010"
     CDS             5109..5972
                     /locus_tag="PLJ75_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04609.1"
                     /translation="MKHAADVITHRFKSSINTLALYALEHELSNDLIASEIDKRFNNV
                     KSIKKDKIMHDVLQSCYKLVWNVNNTFEAGFAAKKIQNDYSECLNNCNAIIIARCILN
                     ILKNTNQKPKKNERLSADFYSQCVNSLLSNPLLQYDSDLLTIYCKQTGIILKVAGIIN
                     ENKIMEINSDDLYRQMFLAFWNKCNWKSLFPSGGYISNEIKNSRQLLLDIIVNTNKSV
                     QLDEVAQTFFELTGIAKYNDLFAVSLLDFSVITWLSFFGIIRYTENSFDKPVTIALTQ
                     HAFPLLQFLLC"
     gene            5978..7153
                     /gene="galK"
                     /locus_tag="PLJ75_05015"
     CDS             5978..7153
                     /gene="galK"
                     /locus_tag="PLJ75_05015"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPD04610.1"
                     /translation="MPSVVDTAITHHKEYFPQSSDVSVFVAPGRINIIGEHIDYNGGT
                     VFPIAIDKHIYICASRNNDGIMRFASYQQHEQGEIAISNIHLPNIRNVAKHKWLVYPL
                     GACVLSDIHFESGFDISFYSTLPVGAGMSSSAAITVGTLYALCTLHGKKMKNIDVALQ
                     AQQVEWDFAGVQCGIMDQMAIVNGRKNNGLLLNTKTMEFQYIPIDAFAIRFVLVNSGV
                     KHSLRESGYNERRNECERALTLLQKSGFAINCLCDCSIDELATIKDILPITEYKRVFH
                     VISENHRVHEFAQEIQKYNLIKAGKLLYQSHHSLSNYFEVSIPLIDAMVEWISEIDGV
                     YGSRLMGGGFGGCTVTMVALYAVEHFKKTIAERFEKKEGRTPDIYECTIDDGVHQLL"
     gene            7253..7969
                     /locus_tag="PLJ75_05020"
     CDS             7253..7969
                     /locus_tag="PLJ75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPD04611.1"
                     /translation="MKKNTLGQLQKNTLREQIVTLLAKKIINNAIQPGDKLPSERDIA
                     ADLDVNRATVREALKKLETLGLIEIRHGEGIFVNDYRSSGNLELFRMMIYLNDTIPLP
                     ILQDVLEMRKIIVPEMAARAVKECSTSDLEELARIINEQEDILEKDLAVHHFIAKTSN
                     NMLYIFILHFFNQIFRDFGYLYFENPDNRKRSEQFHQQLYKAFQDKKAEKAFSITRDV
                     LEYTEKRIFEYYTKTYSNKG"
     gene            7974..9602
                     /locus_tag="PLJ75_05025"
     CDS             7974..9602
                     /locus_tag="PLJ75_05025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPD04612.1"
                     /translation="MKRFIEENNKEQFDVIIIGGGITGAAVAYEAATRGLHVALLEKK
                     DFGWATSAATSKLIHGGLRYLANMEISLVRESLRERRILENIAPTLVYPFPFMIPNYK
                     RLLNNRWVLKMGMIMYDILSFDKGWTWDRSKKIPVHSQISREKALQEEPNLKSEGLTA
                     ATIYYDCQSIFPERLTLSFIKSAVNYGAKVANYAEVKDFLYRNNSIAGVIVYDTLNNK
                     EIDVEGKLVINCGGPWADIILKKAENGTVKHQIRRSEGIHIITKPIVHKYAVTLMSKK
                     GRHFFLIPWRGYTLIGTTDKEYIGNPDNYRVTRESIQELLDDVNEYFGDGTLSYNDIK
                     YTYGGLRPLVDDQVEGTYQSSRRYEIYDNSEEGVQGLITVEGGKYTTSRNLAVKVMKV
                     VEKKLQYSLPKSITHKQYLAGCEIKDLEIFFNTVYTSNSLLDKKSLEVLAKYFGTEVD
                     TVIKIAGSNKKFREIVTHDGEILAEVIYAIRHEMARTLPDIVFRRTGICTAGNPGNDK
                     LTLVAETAAKELRWDNKELSKQLEEVKQRLQIPK"
     gene            9669..10148
                     /locus_tag="PLJ75_05030"
     CDS             9669..10148
                     /locus_tag="PLJ75_05030"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD04613.1"
                     /translation="MVQVHLLNHFPQYAPILAYWSYREWYTQRDIPFDVIIRSYMERA
                     HSSTIPLAWVAIDEKKLPVGMVSLKNNDLWARTDLNPWLASLYIVPEYRFQGIGTALV
                     QAVIDKAKELGYRTVYLFEGHNDTVDLIHYYTSRGFCILEDALDNDGNPTTIMYSNV"
     gene            10309..11274
                     /locus_tag="PLJ75_05035"
     CDS             10309..11274
                     /locus_tag="PLJ75_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPD04614.1"
                     /translation="MYTIVFLDAATVDFGDIDLTPLSQAGRLITYSITKPEEVIERSK
                     AADIIITNKVVFTRKEIECLEHCKLIAVAATGYNTIDIEAAHSRGIAVANVPGYSRES
                     VAELTMCFILSLACNLINYNEATHDGSWSNSPIFTMGKWPTMCISGKVLGIMGYGDIG
                     KRVAQLAAVFGMRIIAFKRGNALSGDSSIPRYTADEFCKLSDFITIHMPLNDYTYHCI
                     NKDFILKMKPTAYLINMARGPIIDTKDLAWALNNNVIAGAALDVLEKEPPDIDDPILK
                     APHCIITPHIAWASKETRQVLINEIAANIHAFFNGKQRNIVNATV"
     gene            11285..12124
                     /locus_tag="PLJ75_05040"
     CDS             11285..12124
                     /locus_tag="PLJ75_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPD04615.1"
                     /translation="MIVGIVGAGTMGTDIAHLAIEHNYHVIVHDTDETQLKLAYEIIT
                     KRLDRYIAEKRLEQTKRGEFISRLKLHKNIKDMRNADIIIECIPEDIALKREVFKALD
                     AVCKSGAILASNTSSLSVTTIAAATKRPEYVVGIHFMNPARVMRLVEIVGGIATTRET
                     IESVKSFVKSLGKTPVESKDFPGFVLNRILMPMINEAIFTLYEGAANAETIDKTMTLG
                     LNLPMGPLQLADMIGLDVVLAITDELYKGFGDPKYRPCPLLKKYVEAGYLGRKAGRGF
                     YTY"
     gene            complement(12136..12975)
                     /gene="nadC"
                     /locus_tag="PLJ75_05045"
     CDS             complement(12136..12975)
                     /gene="nadC"
                     /locus_tag="PLJ75_05045"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPD04616.1"
                     /translation="MEQPSYEELSPIITMALQEDIGSGDITSLALFDEYQYACARISS
                     KNDGIFCGGFLIEYIYHTIDPAISVTIYCNEGAAIKKDDVVCDIEGSVRALLSAERTV
                     LNFLQRTCGIATRTARLVSLVSDTPIKIFDTRKTVPGFRLLDKYAVYCGGGTNHRMGL
                     FDMILIKDNHIKAAGGIQQAVMMVRQKYANRYTIEVEVETLNQVHEAVEAGVDIIMLD
                     NMTKDRISLALSIINGKAKVEVSGNMDEHTLKEIADLPVDYVSVGALTHSVKAFDLTM
                     RLI"
     gene            complement(12999..>14150)
                     /locus_tag="PLJ75_05050"
     CDS             complement(12999..>14150)
                     /locus_tag="PLJ75_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD04617.1"
                     /translation="RTLYDIEMLRTVGYCNGIENYSRIIDRRQPGQPPHCLLHYFKGN
                     YMMFVDESHVTIPQVRGMYEGDRSRKSNLVEYGFRLPSALDNRPLQFHEFETMIDRVV
                     FVSATPAEYELQISAQVVEQVIRPTGLIDPEIEVRPATGQVDDLIGEIKKRVAVNERI
                     LVTTLTKKMAEDLTQYLTEVGLRVRYLHSEIETIERVEIIRDLRKGIFDCLIGINLLR
                     EGLDIPEVSLVAILDADKEGFLRSTRSLIQTSGRAARNSNGKVIMYADTITGSMQEAI
                     DETSRRRKLQMEFNTLHNITPKSISKEIVDIIEREYHSDDVFADLVSEYANAYRTNNG
                     EQLKMVHAKIKQDMLEAANNFEFEKAAVLRDQMFDIEKKIDIVSKASGR"
     assembly_gap    14151..14250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14251..14855)
                     /locus_tag="PLJ75_05055"
     CDS             complement(<14251..14855)
                     /locus_tag="PLJ75_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD04618.1"
                     /translation="MPPFKVISSYTPSGDQANAIEQLADGINKGLKFQTLLGVTGSGK
                     TYTMAKVIEKIQKPTLVLTHNKTLAAQLYREFKEFFPENAVEYFVSYYDYYQPEAYVV
                     SQDLYIEKDASINDEIDRLRLKATSSLVERQDVIIVSSVSCIYGIGNPESFEKNHIRL
                     AAGQEVDRQQLLMKLVSIHYDRNDIAFTRGTFRVRGDTVEV"
     gene            15349..15555
                     /locus_tag="PLJ75_05060"
     CDS             15349..15555
                     /locus_tag="PLJ75_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04619.1"
                     /translation="MLAENNRTSINKTILSLIHKSPGLEERTSRMRDSGDIAGTWSDE
                     EFRGFSLLMKDFEKIDKEVLISGI"
     gene            complement(15609..19484)
                     /locus_tag="PLJ75_05065"
     CDS             complement(15609..19484)
                     /locus_tag="PLJ75_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD04620.1"
                     /translation="MKKNTLVMTALLACIVSFFIQCSNEAPRVFPAPPGGSGGTGIGI
                     DAPDFLGSYPENGDTSIPVDTSVVLVFSEPVDLGTIVPGNVDISGGVTYSASASSDGR
                     TIILTITSGNLSANTAYTVDIDTGILDQDTPQRNLITSYSFTFTTSADTSTFELPRVV
                     AASRYPAPLATNVSINQTFVDVTFTKPIDDTTVVANASFDITPGSGTGITFSPNRQTF
                     RLNLATPLAYGTVHTINLTAAIEDLFSNPLVLDGNHTWTFTTEAAPASGALAISNVWV
                     TDITDTTAILHWTTSIAQAAISIDYGLTSAYGSFASEPAGTRTVHQVSIGGLTLSTKY
                     YFRIQYGAVTATGSFITADNTATSNDKSITDTAGDKSLLTIAQNQTIISVSPYYTQDG
                     SSFIAWKDPDGTVYASYIDNDAAPTKRWGADGSAVDSNNRTVLRVFSDFMGYCFIASG
                     DGTNVYLKRIYNSAGALTFDSTFGANAAAIGLSISTGTNPAVTMLWGNQTANNVSAGF
                     VAKVVPAGAVPNTTEMNIPWANYFFDYDVDLSGVATGNYIIDGSYNQTTVNVTGQNYR
                     HVLGTAANVITATETYRIADNTASTAFTATDHVVNNTTAGTQTPYSNSGTNAYTNHGD
                     NLTGWGTNDLITDGTNWGYLTANAGEVLLTTNLTDSGVSDGFFSFDNYLIRSNTSPVY
                     QYSLVKNEDVPAYSYIANPVVTTETWLPLNDDIFNSTSENYSIWNGTYCWTHVQDPPF
                     DSFYTISFLPSGLISAGETVTLYSYITGGTADTPRTNPLYDDGVDLSAVALNDIVVNL
                     SAYTYSRANNIAYSSAGALGLLANIMSNSQLYWILRFVNPAEPILVIGQATTTSVSQL
                     ISTAKSAGFSGIVEKGDLIYNITDNRYAIVTNVDDNDTLTLNKDIITINDYFIIFASN
                     EPLIESGTVTTTGNPFTDTNANFTSANGAVRGGDIVHNLTAGTSAYVVAVNSATQITL
                     NNNIMTAAGDRYVIIQPRVLVAYERTGDIYGKVIRLRDGSTYLNEFTICNAAGTQANV
                     QLITSGYTSTNPGAIALYRSGAGPTYTYYAKRINGAGVINAADNAANGGLGVQATPAN
                     ATLLAALSDDAGGMFVLYKITNDIYVRRILSTMAISWSNTINNVVDAAICRSATGVIV
                     TYNKTTNSSQLFAQRYTGAGVVEFGETTIVALTPYAYTTNLTITPDRNDGAIISWIDE
                     RYLSQLGYTVMAQAINATGTPQWDADPGAGTDYDGVLIGITNIWYKPYSGLKAAFYND
                     GAPPYGGIFIWYDRRNNKQDIYYDTQPYP"
     gene            19598..21955
                     /locus_tag="PLJ75_05070"
     CDS             19598..21955
                     /locus_tag="PLJ75_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPD04621.1"
                     /translation="MKKLRFYILWLLIIILNTVNSYATISSVVLTPSNPTFLSMGNGY
                     YLAGKTYTFTAQVVDPDVTAWNQITEVVLTIPNSTNIQLRVSAITGTGAGNHTLTIVS
                     GIAGCSSSIAGTWNNFTVTFTVTFQWNTQESAWALNRSVVASATTTVPANNTLTDTKL
                     VSYGICASIRILNFAQDGVSADAMINPWHSAFNVTGTIVYNVPGAAALDAVHLIADAG
                     AGEITNAELRRNGISTVTNDGDETDGLTLTVGAEYFYNLDTTPPYSQLGNHTWTVRIT
                     CATAGGPEVSNNSLTVTCDLVEITNIEIINGGGVNASPDYWRSVNVPGTQVRVTARMQ
                     WAGTNVVGNTTVRMQDSEGNNTDVLIPNGQPNAVANVTYPTVLAQVTDTITYQAITVS
                     GGAYDDEQNVAARIPQPVTRTVYWENADPPGANTPPFTPFSGSSSTAFTITVNWVPLS
                     NPIDGDFDSYRIYYKENSSATWLLLDRTIVAALGNRATGSATITSLKSLTDYDIRISA
                     VDVFGYEVAVANQITTAIATTAGSLEVSITDGITEWGDTTFGTLASNRPLRRTAIRVT
                     INITTDSPDAVNLIVAAGDIGVGVDLIVGGVINPALTENTDYYRIAATRTTSTGWVSY
                     IPDTNPLITLGTDVRFIVETIKSGVASYSDSDSDTEVPPGNPNDLEYSFTVAMQPQFT
                     PWPTRILNNVITNKNPLCYPAYYLTDDALVTITVYDIKGRAVVKLLDKAFRKGGQNIK
                     EGGWNGDNKAGNKLGVGLYYVHIRAKRVSDGKVILNSFQKVVIAK"
     gene            complement(21942..22229)
                     /locus_tag="PLJ75_05075"
     CDS             complement(21942..22229)
                     /locus_tag="PLJ75_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04622.1"
                     /translation="MKVSKDFKELLKLLNKNNIKYITAGGYALGYHSRPRYTEDIDIW
                     IEASYENAEKNILDVEALFATNLPMNREYIQHQLAELHITPHQKIINIILQ"
     gene            complement(22177..22365)
                     /locus_tag="PLJ75_05080"
     CDS             complement(22177..22365)
                     /locus_tag="PLJ75_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04623.1"
                     /translation="MNRVITIFNSFEEAENNEVEYWKSLDPNKKLTILEEIRQRHMEI
                     TGEGKQGFQRVIKIIKQK"
     gene            complement(22473..23459)
                     /locus_tag="PLJ75_05085"
     CDS             complement(22473..23459)
                     /locus_tag="PLJ75_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04624.1"
                     /translation="MTKENGNKPYALILAGYDKPDRSTMIKRKKQIIEAYDGDSIYIG
                     KNKFLQQLAGKPVIQYVLDAVYNAKKKGKPLYDAIYVYNDVKNFTDVIDTRKYHNLHV
                     MQMKQSVGGHLKDFYFNHLEYGRRVDIFFGDTPRITSEDVEWINSQYDTILGKAKDYR
                     GITAHVAYGIVEYEDLKDDTWLNHRLKIIKRGQNKGKLKNFVGFTTFEARVGNSAALL
                     KHRSLDSAVEYEIIDFFYSLRKALAPNIFSKIIYYLWKTKNFILIRQIKRHCIEEERF
                     YKAAINVIERVYNLDLSKFGGYIFHIKHNAAHWENDIDGPKDLKTFRAHIKK"
     gene            23648..25624
                     /locus_tag="PLJ75_05090"
     CDS             23648..25624
                     /locus_tag="PLJ75_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HPD04625.1"
                     /translation="MKKYVIFILIAFVACATGKNTAHTKSLSKELKSLVSQSCFEVVV
                     NKPEKDTLTYEKELPWHLIPYNIRVDKYYSIGTAFAVSPTELLTAFHVLDLKTDSLIY
                     KDFYIRDTQGNVYEIDKILAFDSHRDFIRFTVKNKTFTKYLKLKEEHVVGDTVYAVGN
                     AYGEGIVMRQGELIGTYPEPEDNRFKLLKSSSDVNFGNSGGPLVDDDANVVGIVIQRK
                     DNIAYSLPVSEVSKTGNRGVFHVKMNFGFTLLPEKTIVKDYDDTLQLPMGYKEIKKTF
                     NQRFKKFYTDTMDQFLAQEGKGMFPNGDESLIPLFDFTDSYLLQVNYLSKDNNKWAIS
                     NIEYKETKIRNNGVIRIANPDKRLLFIDISRPDDVSMMDMLNDPKVGMRLVLEGQSIT
                     RDFGDVKTRILSLGDPIATEYHTDNYQRKWRLCIWVMEYSDEAIISCTTPTPEGMAVI
                     VFVCETSEIEFWKYDTLKLLNFIQLSYFGKLKDWNDFLSVNNTMLPSVLSNVTLNYQV
                     KKNLQFNSIDFTINVTNDSIAINNDVKMGVTFGYIRNNNAARWTIRRVMLSEDQKDNY
                     FVLINWYAPDTRLPKDYITYWDSIVKGEHPYTMKPFASNSTTKIAAVVKKIQDQSEKV
                     TKLYTLFLGKAGKVEEEKMIQDFKILMNGITINK"
     gene            complement(25668..27125)
                     /locus_tag="PLJ75_05095"
     CDS             complement(25668..27125)
                     /locus_tag="PLJ75_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HPD04626.1"
                     /translation="MPLKTPSDYVESIRSLNRTIYMFGQRIENPVDHPIIKPSLNAVA
                     MTYALAQKDEYKEIMTAQSHITGNIINRFTHIHQSIDDLVKKSKMGRLLGSQTGCCFQ
                     RCVGMDALNALSMTTYAIDQTYTTNYYQRFLHYLEYIQNNDLVCDGAMTDPKGDRSLP
                     PHKQDDPDVYLRVIKELQDGIIVRGAKAHQTGAINSHEIIVMPTIAMREEDKDWAVAF
                     AVPADTPGIIYIYGRQSCDTRKLEKYTIDQGNALFGGHEALIVFDDVFVPWDRIFMYK
                     EYDFAGHLVERFASYHRQSYACKVGVGDVLIGATQTIAEYNGTEKASHVKDKIIEMIH
                     LNETLYCGCIACASEGKCEQPGTYMVNTLLANVHKQNITRFPYEIARLAQDIAGGALV
                     TLPSADDLYHPEAGKWIKKYFKTKNDVPTENRIKLFRLIENITMGTAAVGYLTESMHG
                     AGSPQAQRIMIARESNVKEKQQLAKRLAQIIASEQ"
     gene            complement(27464..28201)
                     /locus_tag="PLJ75_05100"
     CDS             complement(27464..28201)
                     /locus_tag="PLJ75_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPD04627.1"
                     /translation="MNYRTITIPYKQPIDAIVYSANNSKGTVVYSHGLFSSKDAYKIN
                     QIAPALCQSGYTVISFSYCYTRDYASGTPLTIELPACAHELNAVLEYAANHYNANLHI
                     VGSSMGGVIALYYCAMMPYTVSIKSVSIIATPVHLQHLVTTLAPLGINGDSVAVGEYT
                     IAASSLNQLNSIQLIHLLPRIDVPVCIIHGNNDTVVPVTHAYDIQKNLCVPHTMHIIP
                     DGDHTLTSTKHVDLILQYILSWLTMHP"
     gene            complement(28210..28665)
                     /locus_tag="PLJ75_05105"
     CDS             complement(28210..28665)
                     /locus_tag="PLJ75_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04628.1"
                     /translation="MKRIYRYYCIVTAVLVMIAGNSAALEFTDFHGRWKLLYRGNYGY
                     EFRFAKNYQAVCILYLQNNAIVFKGVYTLDNPQTLRINISQMKNVDNINTVYGAGGFS
                     NVKSSYFLFHAALVKKDTKKMLELKPLSIVIDGITSEGYFEPVILLQKQ"
BASE COUNT         8744 a         5296 c         5723 g         8765 t
ORIGIN      
        1 tatttcatta ctgtatttat ccttcttaca aaaaaaggaa gtacccccta tgaagctttt
       61 tatacttatt ttttatggtc tgatattatt attgctctca atatttggag ctcaccgcta
      121 ttatacgatg tatcttttca ttaaacatag tagaaacaaa gttaaaccaa aagctgtttt
      181 taagaattta ccaaaggtta ccgttcaatt gcctatctac aatgagcaat atgtggtgga
      241 gcgccttatc aacgcggtaa cacagttaga ataccccagg aagaaattag agattcaggt
      301 tctggatgat tctactgatg aaacatcagc gttagctgaa aagatctgca aacaaaagcg
      361 taaagaaggc tatactatta tctatatcca ccgaacagat agaacgggtt ttaaagccgg
      421 tgcgctggaa aatgggctta aatatactga cagcgagttc attgctgtat ttgatgccga
      481 ttttatgccc aatcctgact ttttacaaaa aactatacat tattttactg acccaactat
      541 cggcatggtt caaaccagat gggaccatat taaccgccat tattcactat taactgagtg
      601 ccagagtatt ttattagatg gtcatttcat gattgagcat actgccagga atcgttccgg
      661 aagatttttt aactttaacg gtactgccgg tgtatggcga agacaggcta ttattgatgg
      721 tggaggctgg cagcatgata caatcactga agatttagac ttgagctacc gttcgcaaat
      781 tagtggctgg aaatttttgt atcttcctga tgttacggta cctgccgaat tgcctatcga
      841 aatttcagct tttaaagcac aacagttcag atgggcaaaa ggctctatcc aggtcttcaa
      901 gaagctttat aaaactatcc tcaatgctca acttccattg aaaatcaaga ttgaatcatt
      961 tatgcattta ggtgcaaact tcgcttactt gctcatggta tttcttaccc ttctgatgcc
     1021 tgttgcccta tgggttcgat attatggcgg atttaaaaaa tttgcatatt tagatttgcc
     1081 tatttttata ttagcaacgc tatcagtaat aattttttat ttttacactg aattatccat
     1141 tctttatcat aataaaaata gctttgattc aaagtttaaa ccattggtct atctgccaat
     1201 ggtacttgct gttggtatcg gcttgtccat caacaacagt aaagctgtca ttgaagccct
     1261 gttcaataaa aaaagtgatt tcaacagaac accaaaatat aatgttgcat cattgcaccg
     1321 tatcaatgca gtagagcatg taaagacttt gttaaagaat aaatatagcc tccataccat
     1381 taatattgta tcaatcattg aatttattct ggggctgtac atgtcccttg ctgtgtattt
     1441 tgcttttgat aaaaacctct atgtttcaat accacttata atgctatttc aggttggtta
     1501 tctttatact tcattattta ccataatcaa tagtattact caccatatta ttgttatagc
     1561 caatagttta cgtcgtggtg tggtgaatga ttaaataatt atttctgtca acagcagttt
     1621 ataacactcc tcatagccca gcattgtcat tgttccttcg ctataaaaaa tttacttgca
     1681 caaagtagat tattgctcca tcatagctat gtaaaaaatt aatgtgaggg aagcattatg
     1741 aatagtgtca ttcgttttgg tgtatcaata gatgaaaagc ttcttgatag gtttgatgcc
     1801 ttaatcaatg aaaaaggata tgtcaatcgt tcagaggcaa tacgtgattt aatcagagat
     1861 atgcttgtta aagaagatgt ttccgacccc aatagtgaag ttatcgggac attgacatta
     1921 gtttacagcc atgatgttcg tgagatagct gataaactca atgacctgca acatgacaat
     1981 tataaataca tcatttcatc agtacatgtt catatggatg aacataattg tttagaggtt
     2041 ttattaatgc ggggtaccgc tcaacaggtt aaaaaaattg ctgataattt gctggctata
     2101 aaaaatgttc gacatggcaa gttaactgta acaacaactg tcgactatca tgaataaaaa
     2161 ataattaatt actcaattct gcagaataac tatccgatat aatgataagg gttactatta
     2221 cttcccggta atgtgctaac aaacggaaca taaggagatg ttccatgtat tcatggatga
     2281 gtgcctttat tataacacta ttactgttgt tttcaattag ttgtgttcca cctgcatata
     2341 gatccaaacc agatacatcc cgtgataagc acaattacta taagtttgaa cagcctgaag
     2401 agtatacccc ctttcaggat gatactattt atattcaaaa aaataaaaac gatacaacca
     2461 tgtcagtgtg ttatgataaa tatagtaaat atgaagaaga tggtactaaa catatcatga
     2521 gcgatagtta tcagcacatt aatccggcac ctgaaaataa tagtaaatcc gggatatggc
     2581 atactgttaa aaaaggtgaa acattatata gactnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnttatat cttccctgct gaaaccagtt ttacaaaatg ttcaaatgat
     2761 cggtcatatg tcttttctgc ttcttttgag aaggcacaag cagggagttc cctgcttttc
     2821 aggtgatatg aaatacaatc acagcataat cctttccgtg agcaaggctc ataagtacag
     2881 gtgcatactt ttagatttcg atctttttta cactccatat cgttatccgt cctaattttt
     2941 tagaattcca aaatgagttc cgcctaaatg gtggattaat tgcactttgt taatatcaat
     3001 gacaccatca ggtgtaaagc aatgtatttt tgcatgagcg catgtaacct ggccaataac
     3061 gatagtatga tcaccagcct ccaccattga ataaagctgg cattctatgt aggcaacaca
     3121 ttcagcaata aaaaatccgt caatgtctga acatggtaag gcagtcagat gtgcttcttt
     3181 acatttgtcc actgttcgtc cggaataagt cccacaaaat ataactgtat caagcatagc
     3241 aaaatcagga atatttatac aaaagctttt ggattctttg attaattcca ggctatatcg
     3301 cttatttgcc agcgcgcatg ataataactt tggattattg cttactggca tatgccatgc
     3361 aacggttgtt atagtctttt tttccttgga ctgagctgaa atcaatataa cattgcctgc
     3421 attaatgagt cgatgagaat cctgtattgg taatggtttg aattcattca tcaaggatat
     3481 ccctttcatt tgatagcgtt tttactgcca tgaatgtatt aaatattgat tctgataaaa
     3541 atttaaaatt tattgttcca taggtatcgc cttctgtatg atagttttta ttcctgtaaa
     3601 atgccgtatc agtaatcatt attgccggaa atccaaattt attaaatgaa taatgatctg
     3661 ataaatttat tccgggtatg gaagctggtc ctatcatatc aactatctta ttttttgcat
     3721 atttattgta ctcctttttc catttatatg catacggtgc atagcttggt aatgaaacca
     3781 cagctaaaaa atttccatat ggtggatatt cgttttgcat actccttaat gggtaatcct
     3841 gctgcattct gttccctgca tatcctatca tttcaaggca gatcattaaa ttaatgtttt
     3901 cacctttatc tttacatccc agcgcatgta ccatgctgcc catatatggt cctgagaaga
     3961 aaggtggttc ctcaagagta aatgcaacaa aacgcaatgt ctttctgaat gtatactgtg
     4021 ataacatcct atgcagttca agcagaccgg ctacagcgga tccattatca tcagcaccgg
     4081 aagtgtcttc tatggtatca taatgtgcgc caataattat tatttcatta ctatgttcaa
     4141 aacctggtat ttcagcaata atattgctta cttccaaatt atctaccaga tatttttctt
     4201 catatacatt gtcattgctt tggtgtaaat aattgtatat gaattggcgg cttttattta
     4261 aattatcata ttttcgcaat gttcgctcgc caatttcttc agataaaaca tgcaaaatgc
     4321 attttgtatt attctgaatt tcctttattt cgttttgtgc tgcatcaatt aaagacaata
     4381 accacatccc cggacctctt gtttttatat ttatgaacgc ccttaaaaat caagtttttc
     4441 gttattaaaa aataaaaaac atgctctcaa tgcttgtgtt cacaaggaaa cattcagaat
     4501 ataattatta gaggttccct tgtttaaata atgttacaca ttaatattaa taaaaaaaat
     4561 cattttatta atgcaagtaa aaaccaaaaa tttttaatat attaatatta gtgaagtaaa
     4621 taaatgtaca gtattttttt acctttacaa ctttttttta ttttaataaa atattaattg
     4681 actaaaaatt aattcatgat agtatgttta tcaattaatt tcataagctg atgtaaccat
     4741 atattgtatt gttttattcc tttttggtac attttattta aaatattttt aacagtggat
     4801 ttccttatgg ctacagggca tcttcctaaa tatgatggaa atgaagcatt attggcattc
     4861 ttatggtgaa gcctatagcc attctaaagc aaaagtgcct gtgctgagct aagttgaagc
     4921 aatgcatgca gtcgttgtaa gggtatgtca tactaagcgt tagcgaagaa tcttgtcatt
     4981 tatgaaagaa tatattcttt gtggttttgc ccctcagaat gacaatatgg acaacaagat
     5041 tcttcagttg ctttgctcat taagaatgac acggtattat tccatttttg agggagtagt
     5101 ttatagctat gaaacatgct gctgatgtga taacgcatcg gtttaaatca tcaataaata
     5161 cgcttgcact gtatgcactt gaacatgaat tgtcaaatga tttgattgca agcgagattg
     5221 ataagcggtt taataatgta aaatcaataa aaaaagataa aattatgcat gatgttcttc
     5281 aatcctgcta taaattggta tggaacgtta acaatacctt tgaagctggg tttgcagcaa
     5341 aaaaaataca aaatgattat agcgaatgtt taaataattg taatgcaata attattgcac
     5401 ggtgtatact caacatactt aaaaatacaa accaaaaacc aaaaaaaaat gaacggctat
     5461 ctgcagattt ttactcacaa tgcgtcaata gtttattgag caatccctta ttgcagtatg
     5521 atagtgattt acttactata tactgtaaac aaactggcat catattaaag gttgccggta
     5581 ttattaatga gaataaaata atggaaataa acagtgacga tctatatcgg cagatgtttt
     5641 tagctttctg gaataagtgt aactggaaaa gcctttttcc atcgggtgga tacatctcaa
     5701 atgagattaa aaatagccga cagctactat tggatatcat tgtaaacaca aacaaatcag
     5761 tacagttgga cgaggttgca cagacatttt ttgaactaac cgggattgca aaatataacg
     5821 acctgtttgc tgtttcattg cttgattttt cggtcattac atggttatca ttttttggca
     5881 taatacgtta tacagagaat tcttttgaca aacccgtaac aattgcatta actcagcatg
     5941 catttccact tttacagttt ttgttgtgtt agtgtctatg ccttcagtag ttgatacagc
     6001 aattacacat cataaagaat attttcctca atcttctgat gtcagtgtat ttgttgctcc
     6061 agggcgtatc aatattatcg gtgaacatat cgattataat ggtggcaccg ttttcccaat
     6121 agctatcgat aaacatatat atatatgtgc atcacgcaat aatgatggca tcatgcgctt
     6181 tgcttcttac cagcaacatg agcaagggga gatagctatc agcaacatac acttgccaaa
     6241 catcaggaat gtggcaaaac ataaatggtt ggtatatcct ttaggtgcct gtgtactgtc
     6301 agatatacat tttgaatctg gatttgatat tagtttttat agcaccctgc ctgttggtgc
     6361 gggcatgtca agctctgcgg caattactgt tggaacgctg tatgcgttat gtactttgca
     6421 tggcaaaaaa atgaaaaata tagatgtagc gttacaagcg caacaggtag agtgggattt
     6481 tgccggtgtt caatgtggga taatggacca aatggcaatc gtcaatggca ggaaaaacaa
     6541 tgggcttttg ctcaacacca aaaccatgga atttcagtat atcccgattg atgcatttgc
     6601 aatacgtttt gtccttgtga atagcggcgt aaaacatagt ttacgggaat cgggctataa
     6661 cgaaagaagg aatgaatgcg aaagggcgct gacgctatta caaaagtctg gatttgccat
     6721 taattgtctt tgtgattgca gtattgatga gcttgctacc atcaaggata ttcttcccat
     6781 tactgaatat aaaagggtgt ttcatgtaat atctgagaac catcgtgtac atgagtttgc
     6841 acaggaaatt cagaagtata atcttataaa agccggtaag cttctttatc aatcgcatca
     6901 tagtctgagt aactactttg aggtgagtat tcctcttata gatgcgatgg ttgaatggat
     6961 atcagaaatt gatggcgtgt atggctcacg gcttatgggt ggcggttttg gtggttgtac
     7021 ggtaacgatg gtagctctgt atgcagttga acactttaaa aaaactatcg ctgaacgatt
     7081 tgaaaaaaaa gagggaagga cccctgatat ctatgaatgc accatagatg atggtgtaca
     7141 ccagctgcta taaaaatcat ttattttttt tcaattaatg tggatttttt gctatatatc
     7201 tggtatattg atagtggttg aaccactgaa ccactaaact aaaggtggca gtatgaaaaa
     7261 aaacacatta gggcaattgc aaaaaaacac cctccgggag cagattgtta ccctgcttgc
     7321 caaaaaaatt attaacaatg ctatacagcc cggggataaa ctgccttccg agcgcgatat
     7381 tgctgcagac ctggatgtta acagggcaac agtacgtgaa gcgctgaaga agctggaaac
     7441 attggggctt atagagatac gacatggtga ggggattttt gtaaacgatt atcgctcaag
     7501 tggtaattta gaacttttca ggatgatgat ctaccttaat gatactattc ctttgccgat
     7561 attacaggat gtcctggaga tgagaaagat cattgtacca gagatggcag ccagagcggt
     7621 aaaggagtgt agcaccagcg atctggagga gttagccagg ataattaatg aacaggaaga
     7681 tattctggaa aaggatcttg cagtgcacca ttttattgca aagaccagca ataatatgct
     7741 ctacatattc attttgcatt tctttaatca gatttttaga gactttggct atttgtattt
     7801 tgaaaatcct gataaccgaa aacgctcaga acagttccac cagcaattat ataaagcatt
     7861 tcaggataaa aaggcagaaa aggcattttc cattaccaga gatgtgctgg agtacaccga
     7921 aaaaagaatt tttgaatatt atactaaaac ttattccaat aaggggtaat gctatgaaac
     7981 gttttattga agaaaataac aaagagcaat ttgatgttat tatcattggt ggcggcatta
     8041 ccggtgctgc tgtggcatat gaagccgcaa caaggggttt acatgttgca ctgctggaga
     8101 aaaaagactt tggatgggct acttcggctg caacctccaa gcttatccat ggaggattac
     8161 gataccttgc gaacatggag atttccctgg tgcgagagtc cttacgtgaa cgaagaatat
     8221 tagagaatat tgcccctacg ctggtgtacc cttttccatt catgataccc aattataaaa
     8281 ggctgctcaa taaccgctgg gttctaaaga tgggtatgat catgtacgat attctttcat
     8341 ttgataaagg ctggacatgg gatagatcaa aaaaaatacc cgtacactct caaattagca
     8401 gggaaaaggc attacaggaa gaacccaatc tgaaatctga agggctgact gcggctacta
     8461 tctactatga ttgccagagt atcttccctg aacggttaac actatcattt ataaaatctg
     8521 cggtgaacta tggggcaaag gttgcaaatt atgctgaagt aaaagacttt ttgtatcgta
     8581 ataactcaat cgctggtgtt atcgtttacg atactttgaa caataaagaa atagatgttg
     8641 aaggaaagct tgtcataaac tgcggtggcc catgggcaga tataatctta aaaaaggctg
     8701 aaaatggcac tgtaaaacac cagattagaa ggtcagaagg gattcatatc atcacaaaac
     8761 ctatagtaca taaatatgct gtaacgttga tgagcaaaaa gggcaggcat ttctttttga
     8821 taccctggcg aggatatacc cttattggca caactgataa ggaatatatt ggtaatcccg
     8881 ataactatcg ggtaaccaga gagagtattc aggaattact tgacgatgtt aatgaatatt
     8941 ttggtgatgg tactttatcg tataatgata taaagtatac ctatggaggc ttgcgacctc
     9001 tggttgatga tcaggttgaa ggtacctacc aatcttcacg gcgatatgaa atttacgata
     9061 attcggaaga aggcgttcag ggacttataa cggttgaggg tgggaaatat acaaccagca
     9121 gaaatttagc ggtcaaggta atgaaggttg ttgaaaaaaa actacaatat tcgttaccaa
     9181 aatccataac tcataaacag tatttagcag ggtgtgaaat aaaagattta gagatatttt
     9241 ttaatactgt ttacacctca aattctttgc tggataaaaa gtcccttgag gtgctggcaa
     9301 aatactttgg cactgaagtt gatactgtaa tcaaaatagc tgggtcaaat aaaaagttca
     9361 gggagatagt aacccatgat ggggaaattc tggctgaggt aatttatgct atccgccatg
     9421 aaatggctcg tacgttgcct gatattgtct tcaggcgaac aggtatttgc acggctggaa
     9481 atcccggtaa tgataaactg acactggtgg ctgaaacagc agcaaaagag ctgcgatggg
     9541 ataacaaaga attgtcaaag caactggaag aagtgaagca gcggctacag attcccaaat
     9601 agtagttgcg aacaacaacc attgttgcta ttcatgtcta taactatcaa tcaataagga
     9661 tggcaacaat ggtgcaggta catctattga atcattttcc ccagtatgcc cctattttag
     9721 cctattggtc atatcgtgag tggtatactc aacgtgatat accgtttgat gttattatcc
     9781 gttcgtacat ggaacgtgcg cattcatcaa ccattccact ggcatgggtt gccattgatg
     9841 aaaaaaaact tccggtaggg atggtttcat tgaaaaacaa tgatctgtgg gcacgaaccg
     9901 atctcaatcc atggcttgcc tctttatata ttgttcctga ataccgcttt cagggtatag
     9961 gtactgctct tgtacaggca gttattgata aagcaaagga attagggtat agaacagtat
    10021 atctctttga agggcataat gatacagttg atctgattca ctattatact tcacgcgggt
    10081 tttgcatctt agaagatgct cttgataatg acggcaatcc aacaacaatt atgtatagta
    10141 atgtataaat aaactattga catttaatcc ataattgcgc tttatttaaa atcttagcct
    10201 gtcctaatgt aatgttattt tctgggattt tacgatggtt atattgcaga cgctcaaaag
    10261 gaatgaaaat gcaagggcac ataacttttt tttggaggat ggatatccgt gtatacgata
    10321 gtgtttcttg atgctgccac ggttgatttt ggcgatattg atctgacacc actttcgcaa
    10381 gcaggaaggc tgataaccta ttccataacg aaacctgaag aggtaataga gcgtagtaaa
    10441 gctgcggata tcattattac caacaaggtg gtttttacac gtaaagaaat agagtgtctg
    10501 gagcactgta aacttattgc agtggcggct accggataca ataccattga tattgaggca
    10561 gcacacagca gaggaattgc tgttgcaaat gttccgggct attcccgtga atctgttgcc
    10621 gagcttacca tgtgctttat actgtcgctg gcatgcaatc tcattaatta taacgaagca
    10681 acccatgacg gaagctggag caactcacca atctttacca tgggaaagtg gcctactatg
    10741 tgcatttctg gcaaggtatt gggcattatg ggctatggtg atattggtaa acgtgttgca
    10801 cagttagccg ctgtctttgg gatgcggatt atagctttta aacgaggcaa tgcattgtca
    10861 ggtgatagtt ctataccacg ctatactgct gatgaatttt gtaaactatc cgactttata
    10921 actatccaca tgccactcaa tgattatacc taccattgta tcaataaaga ttttattttg
    10981 aagatgaaac caacagcata tcttatcaat atggcacggg gtcctatcat tgatacaaag
    11041 gatctggcat gggcattgaa caataatgtc attgccggtg cagcactgga tgtactggaa
    11101 aaagagccac ctgacattga tgatcccata cttaaagcgc cacattgcat cataacccct
    11161 catattgcct gggcttctaa agaaactcgg caggttttaa ttaatgagat tgcagcaaac
    11221 atccatgcat ttttcaatgg taaacaaaga aatattgtga atgccactgt atagggaggt
    11281 ttctatgata gtaggaattg taggtgcagg taccatggga acagatattg ctcatctggc
    11341 aattgaacat aactatcatg tcatagtgca tgatactgat gagacgcagt taaaacttgc
    11401 ttatgaaatt ataacaaaac gccttgatcg atatattgcc gaaaagcgtt tagagcaaac
    11461 aaaacgtggt gagttcattt cacgtctgaa attgcataaa aacataaagg atatgcgcaa
    11521 tgcagatatc attattgaat gtatccccga ggacattgca ttgaagagag aggtatttaa
    11581 agcgctggat gctgtatgca aatcaggggc tattttagca agtaatacat catcattgtc
    11641 tgttaccact attgcagcag ctactaaacg tcctgaatat gttgtcggga tccattttat
    11701 gaatcccgcc cgtgttatgc gattggtgga gatagtaggt ggtattgcta cgactcgtga
    11761 aaccattgaa tcagtgaaaa gctttgttaa atctctgggg aaaacacctg ttgaatcaaa
    11821 ggattttcca ggatttgtat taaacagaat tcttatgcca atgattaatg aagcaatatt
    11881 tactctgtat gaaggggcgg cgaatgctga aactatcgat aaaactatga cattgggatt
    11941 gaatctgccc atgggaccgc tgcaactggc tgatatgata ggactggatg ttgtccttgc
    12001 tattactgat gaactgtata agggatttgg agatcccaaa tatcgtccgt gcccgttgtt
    12061 aaaaaaatat gtagaggcag gatatttagg aagaaaagca ggacgaggat tttacaccta
    12121 ttaaattaga ttttattaga ttaaacgcat ggtaagatca aatgctttga ccgaatgagt
    12181 caatgcgcca actgaaacat agtcaaccgg taaatcagct atctctttca acgtatgctc
    12241 atccatattt ccggaaactt caaccttagc cttaccattg atgatggata atgccagtga
    12301 aatcctgtct tttgtcatat tgtcgagcat gatgatatca acaccagcct ctaccgcttc
    12361 atgtacctga ttcagcgttt ctacctctac ctcaatggta tacctgtttg catacttctg
    12421 ccgtaccatc attactgcct gttgtattcc gcctgcagcc ttgatatgat tatccttaat
    12481 caaaatcata tcaaaaagtc ccatccggtg gttggttccc ccgccgcaat aaacggcata
    12541 tttatccagt aatctaaacc caggaactgt tttgcgtgta tcaaatattt ttataggtgt
    12601 atcactgacc agtgaaacaa ggcgtgctgt ccgtgttgca atgccacagg tacgctgtaa
    12661 aaagttaaga acggtacgct cagccgaaag taatgcccgt acactgcctt caatatcaca
    12721 cacaacatca tcttttttta ttgcagctcc ttcattgcag tatatcgtca ctgaaatggc
    12781 aggatcaata gtgtgataaa tatactctat caaaaaccct ccgcaaaata tcccatcatt
    12841 ttttgatgaa attctggcac aggcatactg gtactcatca aatagtgcaa ggcttgttat
    12901 atcaccgctg ccaatatctt cctgtaatgc cattgttata attggagata gctcctcata
    12961 cgatggttgt tccatagtat cataccctta tatagtatct atcgcccgga tgctttactc
    13021 actatatcga tcttcttttc tatatcaaac atctggtctc gcaaaacagc agctttttca
    13081 aattcaaaat tgtttgccgc ttcaagcatg tcctgtttaa tttttgcatg taccattttc
    13141 agttgctcac cattgttggt gcggtacgca tttgcatatt ctgatacaag atcggcaaaa
    13201 acatcgtcag aatgatattc acgctcaata atgtcaacaa tctctttgga aatgcttttt
    13261 ggagtaatgt tgtggagggt attaaattcc atctggagtt ttcggcgcct gctggtttcg
    13321 tctattgctt cctgcattga cccggtaatg gtgtcggcat acatgatgac tttcccgtta
    13381 ctattgcgtg ctgctctgcc tgatgtttgt ataagcgagc gtgttgagcg taaaaatccc
    13441 tccttatcgg catctaggat tgcaacaagt gaaacctcgg ggatatcaag accttcgcgc
    13501 aaaaggttaa taccaataag acaatcaaat atgcccttcc gcaaatcgcg gataatttca
    13561 acgcgttcaa tggtttctat ctccgagtgc agatagcgaa cccgtaatcc tacctcggta
    13621 agatactgtg tcaggtcttc tgccatcttt tttgtcaatg tggttaccag tatacgttca
    13681 tttaccgcaa cccttttttt aatctcgcca atcaggtcgt caacctgacc ggttgcaggg
    13741 cgcacctcta tttcgggatc tatcaaaccg gtggggcgta taacctgctc aacaacctgc
    13801 gctgatattt gcagttcata ttctgcaggg gttgcggaaa caaacacaac cctgtcaatc
    13861 atggtctcaa attcatggaa ctgtaagggg cggttatcca gtgctgaagg aagcctgaat
    13921 ccatattcaa ccagattact tttacgggat ctatcgccct catacatccc ccgtacctgc
    13981 ggtatggtga catgtgactc gtcaacaaac atcatgtagt tgcctttaaa gtagtgcaac
    14041 aaacagtgcg gaggctgacc gggctgtctg cgatcaatga tgcgtgaata attttcaatg
    14101 ccgttgcaat atcccacggt gcgcaacatc tctatgtcat agagcgtgcg nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaacctcca cggtatcgcc tctgaccctg
    14281 aatgttccac gggtaaatgc aatgtcatta cggtcatagt gtatggaaac aagtttcatt
    14341 aaaagctgtt gcctgtcaac ctcctgacca gcggcaagcc gtatgtgatt tttttcaaag
    14401 ctttcgggat taccaatacc ataaatacac gaaaccgatg acacaataat tacgtcctgg
    14461 cgctcaacca gtgatgaggt tgcttttaaa cgcaggcgat caatctcatc gttgatactg
    14521 gcatcttttt caatatataa atcctgggaa accacgtatg cctcaggctg gtagtagtca
    14581 tagtatgata caaagtattc cacggcattc tcagggaaaa attctttaaa ctcgcggtac
    14641 agttgtgcag ccagtgtttt gttatgtgtc agtaccaggg ttggcttttg aatcttctca
    14701 atgacctttg ccatggtata ggttttaccc gagccggtga caccaagaag cgtctggaac
    14761 ttcaaacctt tgttaatccc atctgccagt tgttctatgg cgttagcctg gtcgcctgaa
    14821 ggtgtatatg atgaaataac tttaaatggt ggcatagtgt ataaaactca tgataatgat
    14881 agctatgtac aacataaaat aatactaaat tcaggtaaat caaaaaagat tttgggcgat
    14941 acttactttt tatatatacc attgcatgtg cactaattca tttttatgca ctctggttac
    15001 agcaaacgta aatccagcag caacttgcgt aacgtgatat gatggattct gttgattgtc
    15061 atggagcatg acagaaagtt actggctata gaagcaccgt taatacataa tccagccaca
    15121 gatgatggta aacacttaca tatattccat ggagaatatc atcaagccat acaatgcgtt
    15181 ttactatgta gtggcacaaa aatataaaat actatctaaa tgaagtaaat tatttgataa
    15241 acacttaaag aatagttatg atagtatcat agaatgatat catagaagca tataatgaat
    15301 caaattactc ttagacaaat gccaaaaaga acatatcatc agttgtgtat gcttgcagaa
    15361 aataacagaa caagcattaa caaaacaata ctttccctta ttcacaaatc tcctgggctt
    15421 gaagagagaa cttcaaggat gagggatagt ggcgatattg ccgggacatg gagtgatgaa
    15481 gaattcaggg gattttctct cttgatgaaa gattttgaaa aaatagataa agaagtcttg
    15541 attagtggaa tataaaaaag tcagagcaaa gtttgtatag tttcgtttta tatgcagata
    15601 aaagtattct acgggtatgg ttgtgtatca tagtagatat cctgcttatt gttgcgcctg
    15661 tcataccata tgaatatacc tccatagggt ggtgcgccat cattataaaa tgccgctttt
    15721 aatcctgaat agggtttata ccatatattc gtgatgccta taagaacacc gtcgtaatcg
    15781 gtaccggcac ctggatcagc atcccactgt ggtgtgcctg ttgcattgat agcctgtgcc
    15841 ataacagtat agcctaattg tgacaggtaa cgctcatcaa tccagcttat gatggcacca
    15901 tcattcctat caggggtaat tgttagattg gttgtgtaag cataaggagt cagagcaaca
    15961 atggttgttt caccaaattc aaccacaccg gcaccggtat acctttgagc aaaaagctga
    16021 cttgaattgg ttgttttgtt atacgttaca ataacacctg ttgctgatct gcatattgca
    16081 gcatcaacaa cattattaat agtatttgac catgagatag ccatggtgga taaaattcta
    16141 cggacatata tatcatttgt aattttatat aaaacaaaca taccgcccgc atcatcggat
    16201 agtgcagcaa gcagtgtagc attggcaggt gttgcctgta ctcccaatcc accgtttgct
    16261 gcattgtctg ctgcatttat aacaccggct ccattgatgc gtttagcgta gtaggtatag
    16321 gttggccctg cgccactccg gtacaatgca atagctccgg gattggtgct tgtgtacccc
    16381 gatgtgataa gctgtacatt ggcctgagtc cctgcagcat tgcagatggt aaattcattc
    16441 agataggtgc taccgtctcg taatcggata accttaccat aaatatcacc agttcgttca
    16501 taggcaacca gcacgcgggg ctggataatg acataacggt ctcccgctgc tgtcataata
    16561 ttattgttaa gtgtaatctg ggtagctgag ttgacagcta ctacataggc actggtaccg
    16621 gcagtaaggt tgtggacaat atcaccacca cggacagccc cattggctga agtgaagttt
    16681 gcattggtat cggtaaatgg attacctgtt gtggtaaccg ttccgctttc aatcaacggt
    16741 tcattgcttg caaagatgat aaaataatca tttattgtga ttatatcttt attcaaggta
    16801 agtgtgtcat tatcatcaac attggtaaca atagcataac ggttgtcagt tatgttgtag
    16861 atcaggtcac ctttttcaac aataccagaa aatccagcag attttgctgt tgatataagc
    16921 tggctgacag acgttgtcgt tgcctgtcca ataacaagta tgggctctgc tggatttaca
    16981 aagcgtaaaa tccagtaaag ctggctattg gacatgatat ttgccagaag tcctaatgca
    17041 ccagcagagc tgtatgcgat attatttgcc cgtgaatagg tatacgcgga aagattaaca
    17101 acgatatcat taagagcaac agcggataaa tccacaccat catcgtacag tggatttgtc
    17161 cttggagtgt cagcggtacc accagttata taggaataca atgtaactgt ttcaccggcg
    17221 ctaataagac ccgaaggaag gaatgatatg gtataaaatg aatcaaaggg tggatcctga
    17281 acatgtgtcc agcaataggt tccattccat attgaataat tttcagatgt tgaattaaaa
    17341 atatcatcgt tcagtggcag ccatgtctca gttgttacca cgggattagc aatataagaa
    17401 tacgcaggga catcttcatt ttttactaaa ctgtattggt ataccggtga agtgttggac
    17461 ctgattaaat agttgtcaaa actgaaaaac ccatcagaaa cacctgaatc ggttaaatta
    17521 gtcgtgagta acacctcacc agcatttgcc gttaagtatc cccaattagt tccatcagta
    17581 atcaggtcgt tggtacccca cccggtgaga ttatcgccat gattggtata ggcgttggtg
    17641 ccggaattgc tgtacggtgt ttgtgtacct gccgttgtgt tgtttacgac atgatcggtg
    17701 gcggtaaaag cagtgctggc agtattgtca gcaatgcggt atgtttcagt tgcagttatc
    17761 acattagctg cggtacccag aacatgacga taattttgtc cggttacatt aacggtggtt
    17821 tgattataag aaccatctat aatataattc cctgttgcaa caccgcttaa atcaacgtca
    17881 taatcaaaaa aatagttagc ccacgggatg ttcatttcgg ttgtgtttgg cacggcgcca
    17941 gcaggtacca cttttgctac aaaaccagcg ctgacattgt tagcagtctg attcccccat
    18001 agcattgtta cggcagggtt ggtgccagtg gaaatgctta acccgattgc tgctgcattt
    18061 gcgccaaagg ttgaatcaaa tgtcaatgca cctgcgctgt tataaatacg tttaaggtat
    18121 acatttgttc catctcctga tgcaataaaa cagtacccca taaaatcaga aaaaacccgc
    18181 agtacggttc tattattgga atcaaccgca ctgccatcag caccccagcg tttggttggg
    18241 gctgcatcgt tatcaatata tgaggcgtat acggtgccgt cagggtcctt ccatgctatg
    18301 aatgaagagc catcctgagt ataatatggt gatacagaaa taatagtttg attttgggcg
    18361 atagttaata atgatttatc tccagcagta tcggtaatac ttttatcatt ggatgttgct
    18421 gtattatccg cagtgataaa actgcctgtt gcagtgaccg cgccatactg tattctaaaa
    18481 taatatttgg ttgaaagtgt taaacctcct attgatacct gatgcacagt gcgggtccct
    18541 gcaggttctg atgcaaaaga tccataagca gaagttagtc cataatcaat ggaaatagca
    18601 gcttgagcaa tgcttgttgt ccagtggagt atagcggtgg tatcagtgat atcagtaacc
    18661 catacgttgc ttatggccag cgcaccgctt gcaggagcag cttcagtggt aaaggtccat
    18721 gtatggtttc cgtcaaggac caatgggttg ctgaatagat cttcaattgc agcggttaaa
    18781 ttgatagtgt gcactgtccc ataggcgagc ggtgttgcaa ggtttaatct gaacgtttgc
    18841 cggttcggtg aaaaggtaat gcctgttcct gaaccgggtg ttatatcaaa acttgcattt
    18901 gcaactacgg tagtatcatc aataggtttg gtaaaggtta catccacaaa agtttgattg
    18961 atacttacat tagtagctaa tggtgcagga taacgggatg cagctacaac ccgtggaagt
    19021 tcaaatgttg aagtgtctgc agatgtggtg aaggtaaagc tataactggt tatcaggttg
    19081 cgttgaggag tatcctgatc aaggatacct gtatctatat caacggtata tgctgtattg
    19141 gctgaaagat tgcctgatgt aatggttaaa ataattgttc tgccatcaga cgaggctgat
    19201 gcactatagg taacgccgcc agaaatgtca acattaccag gaactatcgt acccagatca
    19261 acaggttctg aaaaaacaag gacaacggaa gtgtccaccg ggatggaggt gtcgccattt
    19321 tcagggtatg agcccaaaaa atcaggagca tctataccaa taccggtacc ccctgagccg
    19381 ccaggagggg cagggaatac acgaggagct tcattagaac attgtatgaa aaaggataca
    19441 atgcaggcta ataaagcagt cattacaaga gtattttttt tcatgaacta tcgtcacagt
    19501 aaaggtcatt ataaaaagta caataaaaat attgaccttt ttagctaata tatgatatta
    19561 taaaaaaaag tgcaaattaa ttttctttac atatgatatg aaaaagctaa gattctatat
    19621 actatggttg ttaatcataa tcttaaatac tgttaacagc tatgcaacta tcagcagtgt
    19681 tgttttaaca ccatccaacc caaccttttt atctatggga aatggctact atctggcggg
    19741 gaaaacatat acatttaccg ctcaggttgt tgaccctgat gtaacagcat ggaatcagat
    19801 aaccgaggtt gtactgacaa taccaaattc caccaatata caattacggg tttccgcaat
    19861 aactggtaca ggagccggta atcatacctt aacgatagtt tccgggatag cggggtgttc
    19921 atcatcaatt gccggcacat ggaataattt tacggtaaca tttactgtta cgtttcaatg
    19981 gaatactcag gaatcagcat gggcattaaa cagaagcgtt gtagcatcag caaccacgac
    20041 agttcccgca aataatacat taaccgatac aaaacttgtt tcgtatggaa tatgtgcatc
    20101 aatccgaata ttaaattttg cacaggatgg agttagtgct gatgcaatga tcaatccatg
    20161 gcacagtgca ttcaatgtta caggaactat cgtatataat gttccgggcg ctgctgcatt
    20221 agatgctgtg cacctgattg cagatgctgg cgcaggtgaa attaccaacg ccgaattacg
    20281 acgcaatgga ataagtacgg ttaccaatga tggagatgaa accgacggat taacattaac
    20341 ggttggagcc gagtattttt ataatctgga tacaacacct ccctatagtc aattgggcaa
    20401 tcatacatgg acggtccgga taacctgcgc aaccgcaggg ggtccggaag tttcaaacaa
    20461 ctccctgact gttacttgtg acctggttga aattaccaat attgaaatta taaacggcgg
    20521 aggggttaat gcttctcctg actactggcg aagtgtcaac gttccgggga ctcaagtcag
    20581 ggttacagca cggatgcaat gggcaggtac caatgttgtt ggcaatacaa cagtaaggat
    20641 gcaggatagc gaagggaata ataccgatgt attaatccct aatggtcagc caaatgctgt
    20701 ggcaaatgtt acctacccga cagtacttgc gcaggtaact gataccatta cctatcaggc
    20761 aataacggta agcgggggcg cctatgatga tgaacagaat gtagcagcgc gtatccccca
    20821 gcctgtaaca aggactgttt actgggaaaa tgctgacccg ccaggagcta acaccccacc
    20881 atttacacca tttagtggat catcttccac ggcatttacc attactgtta actgggtacc
    20941 gttaagcaat ccgattgatg gggattttga cagttacagg atttattata aagagaactc
    21001 ctcagcaaca tggctgctcc ttgacaggac tatagtagca gcattgggca atagagcaac
    21061 tggcagtgca accatcactt ccttaaaatc gttaactgat tacgacatac ggatttcagc
    21121 cgttgatgtt tttggttatg aggttgctgt ggccaaccag attactactg caatagcaac
    21181 aacagcggga agtttggaag tttccattac cgatggtatt accgaatggg gtgatacaac
    21241 ctttggtacg ctggcttcca acaggccgtt gcgcagaaca gccatacggg taacaataaa
    21301 cattacaacc gattcaccgg atgcggttaa ccttattgtt gccgcagggg atataggggt
    21361 gggggttgat ttaattgttg gtggcgttat taatccagca ttaactgaaa atacagatta
    21421 ttaccgtata gctgcaacca ggacaacttc tactggttgg gttagctata tccccgatac
    21481 caatcccttg ataacgcttg gaacggatgt gcgttttata gttgaaacta ttaaatcagg
    21541 ggttgcctca tattcagata gcgattcaga tacagaagtt ccgccaggca atcctaatga
    21601 tcttgagtat tcatttacag tagcaatgca accacagttt actccatggc caacccgcat
    21661 attaaacaat gtcattacga ataaaaatcc actatgctat cctgcatact atcttaccga
    21721 tgatgcattg gtaaccatta cggtgtatga tataaaagga cgggctgttg taaaactgct
    21781 ggataaggca ttcagaaagg gtggtcaaaa cattaaagaa ggcggctgga atggcgacaa
    21841 caaagcaggg aacaaattgg gggttgggct atactatgtt catatacggg caaagcgcgt
    21901 cagcgacggc aaggtgatat taaattcatt tcaaaaagtg gtcattgcaa aataatgttt
    21961 attatttttt gatgtggcgt aatgtgcaat tccgccaatt gatgctgtat atattccctg
    22021 ttcatgggta ggtttgtagc aaacaatgct tcaacatcaa gtatgttctt ttctgcattt
    22081 tcgtatgatg cttcaatcca tatatcaata tcttcagtat accgtggccg tgaatgataa
    22141 cctaaagcat acccaccagc agtgatatac tttatattat ttttgtttaa taattttaat
    22201 aactctttga aatccttgct taccttcacc ggttatttcc atgtgtcgtt gtctaatttc
    22261 ttctaagata gttaactttt tgtttggatc caatgatttc cagtattcaa cctcgttatt
    22321 ttcggcttct tcaaagctat tgaatattgt tattacccga ttcatgctta acgtgtacat
    22381 cttatttgtg tgaatgtcaa ctacaattac aacacaatga tgatgctatt gcaccaattg
    22441 tttatgggta actatcaaca aaatgttttg ctttattttt ttatatgtgc tctgaaggtt
    22501 ttcaagtctt taggaccgtc gatatcgttt tcccagtgtg ctgcattgtg tttaatgtga
    22561 aagatatagc ctccaaattt tgacagatct aaattatata ctcgttctat tacattaatt
    22621 gcagctttat aaaatctttc ttcttcaatg caatgccgct ttatctgtct gataaggata
    22681 aaatttttag ttttccataa atagtaaata attttagaaa aaatatttgg tgccaatgct
    22741 ttgcgaagac tgtaaaaaaa atcaataatt tcatattcaa cagcactatc aagacttcga
    22801 tgttttagta gcgcagcaga gttgccaacg cgtgcttcaa atgtggtaaa accaacaaaa
    22861 tttttcaact tacctttgtt ttgtcctcgt ttaatgatct tcaggcgatg attaagccat
    22921 gtatcatcct ttaagtcttc gtattctaca atgccatacg ctacatgtgc ggttatacct
    22981 cgataatctt ttgcttttcc tagtatcgta tcatactgac tgtttatcca ttcaacatct
    23041 tcagaggtta tacggggggt atcaccaaag aagatgtcaa cacgccttcc atactctaag
    23101 tgattaaagt aaaaatcttt caggtgcccg cctacagact gtttcatctg catgacatgc
    23161 aggttatgat attttctggt atctatcaca tcggtgaagt tttttacatc attgtacacg
    23221 tatattgcat cgtacagtgg ttttcccttc ttttttgcat tgtatacagc atcaagaaca
    23281 tactggatta caggtttccc ggccagttgc tgtaaaaatt tatttttccc tatatagata
    23341 ctatcgccat cataagcttc aataatctgt tttttacgct tgatcatggt tgatcggtca
    23401 ggtttatcat atccagcaag gataagcgca tagggtttgt tgccattttc tttagtcata
    23461 attattattg ttgaatagta aataataaat acaatgatat tatcatagcg agtatcagtc
    23521 aagcgtttaa cagatgaaaa taaaatgggg ctatcattgt aatttcactt gacagttact
    23581 ctattaaagg taagaaccaa taaacaaata attttaaata atgaaatata tttttggagt
    23641 aaaaaagatg aaaaaatatg tgatttttat cttaattgca tttgttgcat gtgctaccgg
    23701 gaaaaatacc gctcatacca aatccctttc caaagaatta aaaagccttg ttagtcagtc
    23761 atgttttgag gttgtagtaa acaagcctga aaaggacacg ttaacctatg aaaaagaact
    23821 tccatggcat ttgattccct ataatattcg tgttgataaa tattattcaa ttggcacggc
    23881 gtttgctgtt tcgccaacag agcttttaac cgctttccat gtgctggatt taaaaaccga
    23941 ttcccttatc tataaggatt tttatatacg ggacactcag ggtaacgttt atgaaataga
    24001 taaaatttta gctttcgata gtcatcggga tttcatccgc ttcacggtaa agaacaaaac
    24061 gtttacaaaa tatcttaaac taaaagaaga gcatgttgtt ggcgatacgg tgtacgcggt
    24121 tggcaatgca tatggtgaag gcatcgtcat gcgtcagggc gagcttattg gtacatatcc
    24181 tgagcctgag gataacagat ttaagctgtt aaagagctca tccgatgtta actttggcaa
    24241 tagtggtggt cctcttgttg atgatgacgc taatgttgtt ggtattgtta tacagcgtaa
    24301 agataatatc gcctattcac tgccggtaag tgaggtgtca aagacgggga accggggtgt
    24361 ttttcatgta aagatgaatt ttggatttac attgctccct gaaaaaacga tagtcaagga
    24421 ttatgatgac acgttgcaac tccccatggg gtataaagaa ataaaaaaaa catttaacca
    24481 gcgattcaaa aaattttata ctgataccat ggatcaattt cttgctcagg aaggcaaagg
    24541 tatgtttccc aatggtgatg aatcgcttat cccacttttt gattttaccg acagttacct
    24601 gttacaggtc aattacttga gcaaggataa caacaaatgg gctatctcaa atattgagta
    24661 taaagaaaca aagatccgta ataatggtgt tatacggata gcaaatcctg ataagcggtt
    24721 gctgtttatt gatatttccc gtcctgatga cgtaagcatg atggatatgc ttaatgatcc
    24781 aaaggttggg atgaggctgg tattagaagg gcagagcatt acgcgtgatt ttggagatgt
    24841 taaaacgcgt attctttcat taggagatcc aatagccact gaatatcata ccgataatta
    24901 tcagcggaaa tggcggctgt gtatatgggt aatggaatat tcagatgagg ctatcataag
    24961 ttgtacaacg ccaacacctg agggtatggc agtcatagta tttgtatgcg aaacatcaga
    25021 aattgaattc tggaaatatg atacccttaa actgcttaat tttatacagc tttcatattt
    25081 tggaaagctt aaagattgga atgatttttt atcggtcaat aataccatgt tacccagcgt
    25141 tttgagtaat gtaacattga actatcaggt taagaaaaat cttcagttta attccattga
    25201 tttcaccatc aatgttacca atgatagtat tgcaataaac aatgatgtta aaatgggtgt
    25261 aacgtttggt tatatcagaa ataataatgc tgcccgatgg acaatacgtc gggtaatgct
    25321 cagtgaagat caaaaagata actattttgt gcttataaac tggtatgcac cggatacacg
    25381 tttacccaaa gattatatca cttactggga tagtattgtc aaaggtgagc atccctacac
    25441 catgaaacca tttgcaagca atagtaccac aaagattgcg gcggtagtaa aaaagataca
    25501 ggatcaatct gaaaaagtta ccaagctcta tacattattc ctggggaagg caggcaaggt
    25561 tgaagaagaa aagatgattc aggattttaa aatcctgatg aatgggataa caattaataa
    25621 atagtgaaca ttacacgctg ccttaggcag cgtgtagtaa ataattgtta ttgttctgat
    25681 gcaatgatct gtgccaggcg ttttgccaat tgctgctttt cttttacatt tgattcgcgt
    25741 gcaatcatga tacgttgagc ctgcggtgag cctgctccat gcattgattc ggtcaaatat
    25801 cctactgctg cagttcccat ggttatattt tctataaggc gaaacagctt tatacggttt
    25861 tcagttggaa catcgttttt tgttttaaaa tattttttaa tccatttgcc tgcctcagga
    25921 tgatatagat cgtcggctga aggaagggta accagtgcac caccggctat atcctgtgct
    25981 aaacgagcaa tctcgtaggg gaagcgggta atattctgtt tatgaacatt ggcaagcaac
    26041 gtattgacca tataggtacc cggctgttca cacttgcctt cagatgcaca ggcaatacag
    26101 ccgcagtata acgtttcatt aagatgtatc atttcaataa tcttgtcctt tacatgggat
    26161 gccttttcag taccgttgta ttcagctatt gtttgcgttg cgccaatcaa tacatcgcca
    26221 acgccaacct tacatgcata gctctgacgg tgatatgaag caaatcgttc aactaaatgg
    26281 ccggcaaaat catattcctt atacatgaat atcctgtccc atggaacaaa aacgtcatca
    26341 aaaacaatga gtgcttcatg accaccaaac agcgcattac cctgatcaat ggtatatttt
    26401 tctaacttgc gtgtatcgca cgactgcctg ccatagatat aaataatccc cggcgtatca
    26461 gcaggtactg caaaggcaac agcccagtct ttatcttctt cgcgcatggc gatagttggc
    26521 atgacaataa tctcatgcga atttattgcg ccggtctgat gcgctttggc accccgcacg
    26581 attataccgt cttgtaattc tttgataacc cttaaataca catcaggatc atcctgcttg
    26641 tgtggcggaa gtgagcggtc acctttggga tcggtcatgg caccgtcgca taccagatca
    26701 ttattctgta tgtattctaa atagtgtaaa aagcgttgat aatagtttgt agtatacgtt
    26761 tgatcaattg catacgttgt cattgacaga gcattgagtg cgtccattcc aacacagcgc
    26821 tggaaacaac accctgtttg tgaacctaac agcctcccca tcttactttt ttttactaaa
    26881 tcatcaatac tttgatgaat atgggtaaac cggttgataa tattcccggt gatgtgggat
    26941 tgtgctgtca taatctcttt atattcatcc ttttgagcaa gagcataggt cattgctact
    27001 gcattcagtg aaggcttgat gatgggatga tcaacagggt tttctattct ttgtccaaac
    27061 atatagatgg tccggtttaa actacggata ctttcaacat agtcagaagg tgttttcaaa
    27121 ggcatgacta tctcctcctt atagtatagt taatgtacat tcatgcatat gcactcaata
    27181 ctaaaaatgt caataaaatt tatgaatttg acagtatata tcatacacaa agttaaataa
    27241 ttgtatacaa cgataagtca attctaaaga ggaaagggca ggatgtccaa tatacttttt
    27301 actttcgccg ctaaaagtcc acaactcatt tgtccattgc aaaaactttg catttgaaat
    27361 atcagatatt gaggagtatt ttttatgttt gtttataaat catgaaaata caataaaagt
    27421 aatatgagat tgtgtaaaga gtaaaaaatg agaatagatt aaatcacgga tgcatcgtaa
    27481 gccatgacag tatatactgt aatatcaggt ctacatgctt tgttgatgtt aaggtatgat
    27541 ccccgtcagg tatgatgtgc atggtgtggg gtacacacag gtttttttgt atatcatacg
    27601 catgggtaac gggcaccaca gtatcattat tgccatggat gatacatacc gggacatcaa
    27661 tacgaggcag taagtgtatg agttgtatgc tgttcaactg attgagggat gatgctgcaa
    27721 tggtatattc acctacagca acgctatcac cattgatgcc cagcggagca agcgttgtta
    27781 ctaaatgctg caaatggacg ggtgttgcaa tgatgctgac actttttatt gaaacagtat
    27841 acggcatcat ggcacagtag tacaatgcta ttacacctcc catactggaa cctacaatat
    27901 gaagatttgc attataatga ttggctgcat attccagtac ggcattgagc tcatgagcac
    27961 aggcagggag ctctatggta agcggtgtac ctgatgcata atcccgtgta tagcaatagc
    28021 taaagcttat gacagtatat ccactctggc ataatgcggg ggcaatctga tttattttat
    28081 aggcatcttt agaagaaaaa aggccatggc tatatacaac agtgcctttt gaattattag
    28141 cactatagac aatggcgtca ataggttgtt tatacggaat ggtaatggtg cgatagttca
    28201 tgctatttgt tattgttttt gcaacagaat cacaggttca aaataacctt cggatgttat
    28261 cccgtcaatt actatggaca gaggtttgag ttcaagcatt tttttagtat ctttttttac
    28321 tagtgcagca tgaaataaga agtacgatga tttaacattg gaaaatccac ctgcgccata
    28381 gacagtattg atgttatcaa catttttcat ctggcttatg ttgatacgaa gtgtttgggg
    28441 gttatccagc gtatatacgc ctttaaatac tatagcattg ttctgtaaat agagtatgca
    28501 aacagcctga tagtttttag caaacctgaa ctcatagccg tagttgcctc tatataaaag
    28561 tttccatcgt ccatgaaaat cagtaaattc aagagcagca ctatttcctg caatcatcac
    28621 aagaactgca gtaacaatac aataatatct atagattctt ttcatgcaac taccgcttaa
    28681 caataactat cgccccataa aaggttggta cacattatga ttcagtga
//
LOCUS       DAOOJH010000024        28037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1777_length_28037_cov_9.372597, whole genome shotgun sequence.
ACCESSION   DAOOJH010000024 DAOOJH010000000
VERSION     DAOOJH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1777_length_28037_cov_9.372597"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PLJ75_05110"
     CDS             complement(<1..842)
                     /locus_tag="PLJ75_05110"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPD04629.1"
                     /translation="MKKYNVVILGATGAVGIEFRKILLQRNFPINTIRFLGKTTVGKT
                     IDFDDKKVTVEAVTDDAFKGFDIALFSAGASISREVARKAADSGCIVIDNSSAWRMEK
                     DVPLVVPEVNPDDVDWHNGIIANPNCSTIQMVVALKPIHDVAKIKRIVVSTYQAVSGT
                     GLKAIEELEIQTKDIMNGIPISQRKVYPHQIAFNVLPHIDVFLDNGYTKEEMKMVNET
                     KKIMRDSSIQVTATTVRVPVFYGHSESVNIETQKKITPIEVRELLQATKGITVVDNPS
                     KNEYP"
     gene            1073..2119
                     /gene="argC"
                     /locus_tag="PLJ75_05115"
     CDS             1073..2119
                     /gene="argC"
                     /locus_tag="PLJ75_05115"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPD04630.1"
                     /translation="MKNALIVGATGFGGLGLIEILLRHNGFKIKQLIARKDTGKKISE
                     VFPHMEGFCDMYVQSTEEIDYNDIDIAFFSTPDKAGMQLIQSFYQRNIPVIDFSGDFR
                     FNTINDYNIYAKNKGMNTEHAAPDILPHAVYGLPEKYAEKIKKATIVGNPGCFAIAMI
                     LGLLPAVEEGIIDSETIICDGKTGVSGAGKNPGEANFYPQRYEDVNTYREGYHQHIVE
                     VENIVNAHAKKKHTIFFVPQIVPLNRGILVTIYADAIVTMDNEKLYDVYARYYKGKPF
                     IHITQRSCHTTDVRGTNLCLIRPAIDKRTGKLFITSVIDNLVKGQSGNAVQNANIMMG
                     FDETEGLMVPAFYP"
     gene            complement(2123..2851)
                     /locus_tag="PLJ75_05120"
     CDS             complement(2123..2851)
                     /locus_tag="PLJ75_05120"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPD04631.1"
                     /translation="MNLQIISIVFIIATLAIAFISIFYAISTGERGLSLQQLRELMVK
                     KQIEARGVTDQRVLQAMLKVERHKFVPELSLSSAYEDHPLPIGYGQTISQPYIVALMT
                     ELCELDGTEKVLEIGTGSGYQAAILSLLAKEVYTIEIVEPLGKQAALRLKSLGYNNVT
                     VKIGDGYKGWPEHAPFDVIILTAAPPTIPDALINQLSEDGGILVAPVGDYNQELIKMV
                     RHGNTYSQKVITYVRFVPMIPGKE"
     gene            3213..4955
                     /gene="ptsP"
                     /locus_tag="PLJ75_05125"
     CDS             3213..4955
                     /gene="ptsP"
                     /locus_tag="PLJ75_05125"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPD04632.1"
                     /translation="MSKSIEGVIASPGIVIGKAYIYRGDNIIIPKYTIPAEQVEKEIK
                     RYDESVKKTKEEIKAIQSQIATSLSSDMADIFQSHLMVLEDPYMDQKVRETISKEKRN
                     AEWVLNDFSLQLIQNLKSIEDEYLRERIIDIADINKRLINNLQKTHDESLADLKEEVI
                     IIATDLTPSDTAMMNKKYVLGFVTDRGGRTSHTAIMARALEIPAIVGAQVATSLINHG
                     DMIILDAIHGKIIVNPSKDEISEYEKYRDDLKQLENELATIIELPSHTLDNETVYIYG
                     NIEIPEEMNIIRDHGAQGIGLFRSEFLFLDKSLPDEERQFEEYKRVVEFFTPHPVTIR
                     TLDIGGDKIYAFTKQYKERNPFLGCRAIRFSLENEFLFRPQLRAILRVSALGNVKLMF
                     PMISSVEEFLKAKSILHEEMENLDKAGIPFDKNLPVGIMIEVPSAAINIDIFCKYCDF
                     FSVGTNDLVQYTLAVDRISEKIAYLYNPLNLAVLRLLKQIADVSNQNNIPLSICGEMA
                     GEPKYTMLLIGLGYRQLSMSSAFMYQIKRIIRSVSVKECIEMTDYLLQLHNTYDIENA
                     IMEVLNKKFPNMFF"
     gene            4984..5736
                     /locus_tag="PLJ75_05130"
     CDS             4984..5736
                     /locus_tag="PLJ75_05130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04633.1"
                     /translation="MKLYHDLAEYYFVIEKGNRNINDDVNLILELSNTKKNLLLLDLG
                     CGTGEHLNELSKKGFICTGLDYSKNMLTHAQLRFPNAANFLLCDITNFDFYEEFDVII
                     SLFGSFDYMIDNDDVDKALWNTWRAMKSGGFGLFEIWNAIPVRKIKEKPLTTVSKVKY
                     KNMTIERQRGFTLIPDDNKTITEVNYIYTISENSKREVIKDKHIMRAFYKEEIESFFA
                     NNGLSIINIYANSKKETFNSLSNKILIHFMKE"
     gene            complement(5747..8194)
                     /gene="lon"
                     /locus_tag="PLJ75_05135"
     CDS             complement(5747..8194)
                     /gene="lon"
                     /locus_tag="PLJ75_05135"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018949939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPD04634.1"
                     /translation="MKKDIDFFKRYPLLPLRDMVIFPHMVIPLFVGRDISIKALDKAM
                     KGDRLLVLVAQVDSQIANPKKEDVHDVGTLSEILQLLKLPDGTVKVLVEGIDRVYVSQ
                     FETDDDGITVSIKNIERPYEADSKVEALKRNLIDTFEKYIKLNKKVPQEALTTVMQIE
                     DISHLADVIASYISMRVEFKQAILELGNAIERIEKLIELINGEIEIMYIEKKIQGKVR
                     KQMEKTQKEYYLNEQMKVIKKELGQGEDLADEVEELHKSIEEAKMSKEAKDKALKELK
                     RLEKMPPLSAESAVVRTYIEWLRDVPWFKKTRDNRDIDHAMHVLNEDHYGLEEVKERI
                     IEYIAVRHLSNKLKGPILCFVGPPGVGKTSLGRSVARALGRRFVRMSLGGVRDEAEIR
                     GHRRTYVGALPGRIIQQMKKAGVVNPVFLLDEVDKMSMDFRGDPSSALLEVLDPEQNN
                     TFVDHYLEVPYDLSDVLFITTANVQHKIPAPLQDRMEIIEINSYTEPEKLNIALQFLL
                     PKQIKENGLNESHIQYGNDAMLYTIRHYTREAGVRNLERVIAKVCRKVAREVVKNGPL
                     HIRKITVDILHEYLGPIKFKYGEKEQKSEIGLANGLAWTEVGGDVLQVEASLIKGKGQ
                     LILTGQLGEVMQESAHAAFTYVRANSQYFNIPEDVLRKHDIHLHVPEGAIPKDGPSAG
                     ITMGVVIASLLSQIAVRCDVAMTGEITLRGKVLPIGGLKEKVLAAHRAQIKHLIIPMD
                     NEKDLEKIPEYVRKQLIFHPVTSMEEVLFLSLEEPDKFFKDRQKTEKLTEQWKDTVRE
                     SVISDVTNELLTKHNYT"
     gene            complement(8266..9534)
                     /gene="clpX"
                     /locus_tag="PLJ75_05140"
     CDS             complement(8266..9534)
                     /gene="clpX"
                     /locus_tag="PLJ75_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPD04635.1"
                     /translation="MANKKKDNTKPEVIHYCSFCGKSQDLVRKLVAGPNVYICDECIE
                     LCNEILEEDWALDGGDLLTNNLPKPHEIKAILDEYVIGQEYAKKVLSVAVYNHYKRIT
                     SNFNLRKGDVELDKSNILLIGPTGSGKTLLAQTLAKILKVPFAIADATSLTEAGYVGE
                     DVENILLRLIQNADGDIKRAEIGIIYIDEIDKISRKSENPSITRDVSGEGVQQALLKI
                     VEGTIANVPPQGGRKHPHQEFISIDTKNILFICGGAFVGLEKIIHARIGNKTMGFGAE
                     VKSVKDIGKDNILEKMIPDDLIKYGLIPEFVGRLHVNAVLQELDKHTLRDILTKPRNA
                     LTKQYQKIFGFENVELEFTSEAIEAIAQIAIERETGARGLRSVLEEIMLDLMYEIPSK
                     KTIAKVVINDDVVLHRKPPDIIQRTTEKSA"
     gene            complement(9568..10158)
                     /gene="clpP"
                     /locus_tag="PLJ75_05145"
     CDS             complement(9568..10158)
                     /gene="clpP"
                     /locus_tag="PLJ75_05145"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPD04636.1"
                     /translation="MSYLVPIVVEQTARGERAYDIYSRLLKDRIIFIGDAIDDHIASL
                     VIAQMLFLEAEDPDKDIYLYLNSPGGIVTSGLAIYDTMQYVKPDVATICLGQAASMAS
                     LLLAAGAQGKRSALPNSRIMIHQPMGGFQGQASDIAIHAKEILKIKEKLNEIYTIHTK
                     QPLEKIEKDVDRDYFMDAQEAVEYGLIDKVIRKDSR"
     gene            complement(10257..11600)
                     /gene="tig"
                     /locus_tag="PLJ75_05150"
     CDS             complement(10257..11600)
                     /gene="tig"
                     /locus_tag="PLJ75_05150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPD04637.1"
                     /translation="MEIKEFKLDNARIRLDIEVPGDRIDAEYARVLKELQKKAEIKGF
                     RVGKAPIDLVEKKYSDYVLGETSENIVRSAYVETIEQKGYTPVSQPVFDFEKVEKGKP
                     FSFKIEFDIPPTIELGQYKGSHVEQKVVKVTDEDVEAEIETMREKMATITKKEGDTIK
                     VQKGDQVTLKMKRIDDKSKEEIDAAAFTSHNLIVGKSKESWAIDDDIIGMEKDEEKEI
                     KVKYPKDYEVEDLKGQKVTYLVKVEEIFEFKLPELNDDFAKSVGEYNSVDDLKNRIRE
                     NLETYVKSQTLNETKNKILEMIVENSKFDIPQSLIQKEMYAIFRRLQSRFGNVGNDIN
                     EFASIFGINPEELSKSIMDEADKNIKNAMALSKIAELENLKVSEEQFQKFVKSVAERN
                     TVSEEEVVKTVEEKGNKEEIEGDLILDSAYDFIYEHADIKMSKPITFQEYVNGKK"
     gene            complement(11843..13480)
                     /locus_tag="PLJ75_05155"
     CDS             complement(11843..13480)
                     /locus_tag="PLJ75_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04638.1"
                     /translation="MDKKIVNRNNVYFYTFLFLLVFVLRIPALYHTIVDIDEAAFAEF
                     ANKWINGGIPYVHMYDNKPLLVYFFYYIVFYFFGINNLFAVHAVTIILVYCCAIIIYK
                     FIQNIRGIDEAKAASLFFIFLMHIYEPKYIATNTETLFTLPILISFISYYLFIINKWK
                     TNIALVTSGIFAGISFFINYKAGVILIVYGIFAIFSITKATNKKREFVKQLKDMIVIA
                     TGFLMVVAGATSYLYCNNALDDFITLGFLYNFKYIQSGMTTVPIVKAIARFIIFIVCS
                     LPLWLIAGRHAIHLLKEKQYTPITLISILWLLAGCFSVLLGWRAYGHYFIQLVPPLAI
                     LGGLLIKDITLSLLKKIYIYFIIMMILFTASRINIPLTYHYLGDSNSLSDYAYKNVAH
                     AVTEITAPDDTIFVWGSGAVAYIYANRRCASKIIIADYISGRQFGVSNEDMQANVNAY
                     IQLLRKQFIAEVMKNPPAVVIDTSPSGYYGYDKFPLKNFHALYDFINNNYRFAKRIDM
                     MDIYVLKNITMRHSTIIPVRSNGSKAFGKNQECVAPK"
     gene            complement(13465..15162)
                     /locus_tag="PLJ75_05160"
     CDS             complement(13465..15162)
                     /locus_tag="PLJ75_05160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD04639.1"
                     /translation="MFLNNTVKIFYKLFNKNSFMVFVCICFSTIILRLPSVIIDFIHV
                     DVLTSYLLAKRDLLGLSYSMNKGWLYHQLMKFSINYIGDTPSSFHFIGLLFILTTMYG
                     IYILAKKMYDSKTGLFAAFLYGTVISCYHTEYLAFNGEIFYNLFNVFALYCYYVCVFE
                     KKRIYIVLLVINIVASYLIKFQGIFILPIILTHFIFIYPLSLNTNDYRRFYKNLFIAI
                     ASALVTVATFLIAGYVYFGFDFIDYIYKAIMGKYTYVANRGFNIFSILIKLLWRVYQF
                     SIFHAFFWFPGIILIAKFIYDFKNKKIDYKTLFILYVSVCFFILVFSGLGRLSVHYFI
                     PIMPSIAILSSKQILYVFSERGKRMVFMQLMFTIVFFFAWHYKDLYIYTCNPNLKHNE
                     SRLTEIFRIAIIGSYGEYLLPHKSLLRAIEYINKNHPGSTMLIWPMGTEIDYFTTGES
                     VIPSYWLNERALYAVVQREKGNEHYIHEYENYIIQLIIQYKPDLFVDVGSTDMIKKVM
                     VYRKKGDPLFYFNIHATPIIHFGHFASLDDFPAIIRYLNEHYTFNGYFGKSRIWIKK"
     gene            15389..16480
                     /locus_tag="PLJ75_05165"
     CDS             15389..16480
                     /locus_tag="PLJ75_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD04640.1"
                     /translation="MDNCISIIVPTYKEADNISVLVESIDKAMRSANLNYEIIIVDDN
                     SNDGIVEKVKQLNKQKYPVAIKVRVHERGLSSAVIEGFKLAKGNIYLVMDADLSHPPE
                     KIPDIVQPIISGKAEFVVGSRFVEGGSAEHFNIYRKLNAWISKMLAMPFTKVKDPMAG
                     FFAFDARLLTPEILPILNPLGFKIGLEMIVKCKPKTVVEIPITFKERLYGKSKLSTRE
                     QVLYLLHLKRLFHYKYPSMYQFLFFSIIGASGMIVDLTTVFITYEVLHIKFIIARAIG
                     FIIALTWNFFLNRKLTFPDSPQKNIFKQYRNFFLVCSIGGLFNWFISVFLYWHYSYFN
                     QFYLLAAFIGILGGLLINFFGSKFIVFGK"
     gene            complement(16484..17359)
                     /locus_tag="PLJ75_05170"
     CDS             complement(16484..17359)
                     /locus_tag="PLJ75_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD04641.1"
                     /translation="MKSGLTIIVPVYNEISAIEHSINHLKEIKKKCKNIDMEIIIVND
                     GSTDGTEKILARMPNNKDIKVINHSENQGYGAALKTGIRASKHQYVAITDADGTYPDE
                     RIPEFFEIVINEGLDMLVGARIGESVHIPFIRKLPKWVINKMANYMVGTKIPDLNSGM
                     RIMKKDVVKKYFKILPDGFSFTSTITLAMLSNGCHVKYIPINYNHRKGKSKIRPFYDT
                     LNFFRLIIRTVMFFDPLKIFLPISLPLFGGGIILTLLQAILYQNIHTISVLLTLSGLN
                     ILTVGMIAEMIANKD"
     gene            complement(17480..18454)
                     /locus_tag="PLJ75_05175"
     CDS             complement(17480..18454)
                     /locus_tag="PLJ75_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPD04642.1"
                     /translation="MSKKINYLNGIGIILLAIILWKIDFKQVFKYLKDANYWYLLLAY
                     PFILMNIFLKAVRWNFLMKTQGVRNSIFESFYVYLWAFYFGAVTPGRIGEISKALYYQ
                     EKFDNFGSSLSPVVVDRLYDVGIRIAMIFILYPFYSNLFHFKYVDVIILLSCIIAGIV
                     VLAKYKSIQNIIITFSKFLVPKKYYATIENNISGFINDIIKMVTTVKYLAICIVITIP
                     SFLCYCMVGYFILKSFGINMTFTYNIFCLAVSSFSVAVPISVSGLGVREAVMIFLFNS
                     IGLGRESAVLFSLSIFALSPILGFHGWLVNLIMLIRNHIKKRMKVKVN"
     gene            complement(18502..19146)
                     /locus_tag="PLJ75_05180"
     CDS             complement(18502..19146)
                     /locus_tag="PLJ75_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPD04643.1"
                     /translation="MKKFTLYLLVCFLVYFAIFVIIDFYAEKKYDILYNTINTPFDAA
                     VVFFSDFETHNTIDDESLRRLSFAIELYKQHAINNIIFCGGWRPSINTSGSKLMATMA
                     EEMGVDRSHIYYETHSRDTVQNWKEAEKIIVVKNFKKIILLSSPLHLMRIEKIVTIHD
                     DIDVTYSSYSKRSIIPPKGYLDNFCDYNYNMVSIISYYLLPSELYQYIVTNLRN"
     gene            complement(19155..19955)
                     /locus_tag="PLJ75_05185"
     CDS             complement(19155..19955)
                     /locus_tag="PLJ75_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPD04644.1"
                     /translation="MSEQYYFTLKGFWKRVKQEYDYYTRRPWTLKQVGEFWDTVNDYD
                     AVNEQLYTYHRRFTNSYELAYKHLKKDTYNMIDIQARSGKGSLFWHEKGKVTSSVCVD
                     FSDFLISLADKRLNKTGLQYSIVKVLNFPLPFNDGQFDLVCSYETIEHIYDYSAFFDE
                     LVRIMSKDGIMILTCPNRMWEWVHWLSAAININHSEGPHRFLKRGELIQCIKKNRMEI
                     LEENSTIILPFNNKLSIAINTFLENYLPEFIKRIIALRRTFILGKGDK"
     gene            complement(20047..21408)
                     /locus_tag="PLJ75_05190"
     CDS             complement(20047..21408)
                     /locus_tag="PLJ75_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPD04645.1"
                     /translation="MPLKVKYDFNDLMEKLGGKAMPLCNFCGTCIGVCASNALSADYE
                     KGRPVFDISKCTTCSLCYNNCQGAGVNFSELDNHFRLGERYDNYLGSYKKCFLGHSLD
                     DMIRIQGASGGCVTRLLVYMLEAGVVDGVILPRQKADQPWLFKPEIITNVETVKASAQ
                     SKYCLIPTNEILKTLKGKKGRYAIVALPCQIHSLRKLQHEHNKNAQKIEYILSLICGH
                     NMEYGATTFGMKKLNIKKEDVVDIKYRDYGDWPGGLSFYLKNGERKGINFFNYHYLNA
                     VYLPNRCRLCPDYYGNYSDISFGDSWLLRLLGRGMNEKGIPKGWSSIIARSQKGLSLL
                     EAAQKAHALYLEEIEPPEIHESFPFNISYKLNGIFVRLQFAKKKPEFIGLAKPEKLDR
                     YLYHFIYNIILILGSTSVFKFFLSIMPLNFNLFLIKNFKKLMGYKPNARDRVLQYNEK
                     SNV"
     gene            complement(21550..23187)
                     /locus_tag="PLJ75_05195"
     CDS             complement(21550..23187)
                     /locus_tag="PLJ75_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPD04646.1"
                     /translation="MMGKYKVKYFIIIFWILIFPVALYFFDKYIPVNISLNLLILLFF
                     IALLLYAIYVYSNKKIRIFIIGLLIISIIPSFFIISAFSITNTIYHRSDFDVIFTTDL
                     DESLEFIHQFVNFKIIMLLIAYFGPMPLFFKMKDIQMELNNNKRKLIVMGSFLVLILI
                     FTVNWKLLQKEYHVVDFYKSYYQFINNKNYEIIKAKRADLKWKDKVTCDLKIREHKLY
                     VLVIGESLSRAHMQIYGYQRETNPQLIKIKDELYIFNNVISPAVTTVDVMKYVLSFAN
                     LDHPEYFIEKRNVVNLFNDVGFETIWIGNQSLRGNRNNVSHSIVARECNQYYDVSSET
                     DEVVINKLDTVLTDEPHKNLFIIIHLRGCHTKYSSRYSDIFNYFNHKKIPIPYDYGLD
                     DKQKLIIDEYDNSVRFNDYIIYSIIKRVKSHCKYAWVVYFSDHGEELFEFRNMFGHNS
                     RNFSKYMCEIPFIVWVSNEYKNANKSFFEDIPKYLNRAYSTENVIYSIADLSKLKFTD
                     FDASLSIFSKQYTNHLRKVYNIEYEKIPPITTVNGFK"
     gene            23479..23682
                     /locus_tag="PLJ75_05200"
     CDS             23479..23682
                     /locus_tag="PLJ75_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPD04647.1"
                     /translation="MLTPEELELYQRQLIIKGWGKESQEKIKKTHIFIAGAGGLASTA
                     SLYCAVAGFGTITLCDYDTIIHS"
     gene            complement(24133..26733)
                     /locus_tag="PLJ75_05205"
     CDS             complement(24133..26733)
                     /locus_tag="PLJ75_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016523553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPD04648.1"
                     /translation="MENIVFFNKDFTGVDKKQYKELGIRGRRAADLAKMGLPIAPGFI
                     VDAELTMKLPKVNVKQLIKSYVDKIEKDMKKGYGDTEKPLLLKVVLSSDLNVPFFPSI
                     HNVGLNDAIVTAFAKFTGEDFAWGEYRFLLRSIASKIYGIDEKELDELEGKLPKNAKA
                     KEYKEVVTKYKKFLGDKFSEDVYDQLGLILKGAAARYCDSDIDVDDSLAITIQAMVYG
                     NFGQNSFAGNYFTRNIITGDALIQGDFLQNEFDVDRGTPKDIAKIDKKYYDKLVEIAK
                     KVEDNFKEIRNIKFTIEEGNFWLIEQREVDEKSTQAHIKTLLDLNTRKVVSSNFLIES
                     IKPSQLNELLHPVIDPRTTKNVKSIKGGIAGSTGAAIGRVFFSTDKLLEEYKRSVMHG
                     GDTNLILVMPATFAEDVKAIEVARGVITSEGGFSSHAPVVARSLGKVAMVQPEIKIRG
                     NSFTLAGKTVKEGDYVSINVPYYEPPTIYLDKVGLIEPDFEKNGLIDFLKIVQSFIKD
                     FDVRANADQPRDAKVAKMFFADGIGLCRTEHMFFNEKRIMKFREMILSETEVERRKAL
                     DELKPMQRSDFYELFKIMQGHPVTIRLLDAPLHEFLPRTDDSMKDFIQYMKSRKKGMS
                     DAEIRIRCEELSEMNPMLGHRGCRVAITYPEIYEMQCRAIFEAACMLKKEGIDVVPEI
                     MIPIVMSETEIKFIKNGKRIEGKTVKGIRDIKDEVVEEYGIDDLEYEVGTMIELPAAA
                     VGAGRIAQYAEFFSFGTNDLTQTTYGLSRDDINSFFPSYSLYDLIPNNPFKVLGREVK
                     ELIEMASTRGSLTRPDITMGLCGEHGADPDNIEFCMNVGLNYVSCSPYAIPLSKLAIA
                     QLNIKNAKSK"
     gene            complement(26772..27680)
                     /locus_tag="PLJ75_05210"
     CDS             complement(26772..27680)
                     /locus_tag="PLJ75_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04649.1"
                     /translation="METTFYSLATKTNFNKALNHFYRVNDVVDEIESGRNFADAVNVM
                     LAEGDLTVDQVLPMIGAIIKDKYQYSYDSYNVPVTINDFSKIVEETSKWTALDIILVY
                     FTVNSEPFLINPKNMIHWERVRDIVRDQLMVIYVKKKKEPNPKVENDAINAIEEMMAG
                     KDVFINKEFIDETVAVREAKKQIAPAEAAAAGKKNMTPKYAVTVTNELFHNGNVEAWK
                     RIIESFKVSHPDLDVLIFHEGEKINDINSLFKWGKVKHGDPILFQVVGENIRNVSKLQ
                     KYLFEGASPRFEQFLKIAVGKVLNLF"
BASE COUNT         9272 a         5140 c         4068 g         9557 t
ORIGIN      
        1 ggatattcat tctttgatgg attatcaaca acagtaatcc ctttagttgc ttgtaataat
       61 tctcttactt ctatgggtgt tatctttttc tgggtttcaa tatttactga ttctgaatgt
      121 ccataaaata ccggcacgcg tactgtcgtt gctgttacct gtatagaact atcgcgcata
      181 atctttttag tttcgtttac catcttcatc tcttcttttg tatatccatt atccagaaag
      241 acatcgatat gcggcagtac attaaacgct atttgatgag ggtatacttt acgctgggaa
      301 atgggaatgc cattcatgat atcctttgtt tgaatctcta attcttcaat tgcttttaac
      361 ccagttcccg aaactgcctg ataagtgctg acaacaatgc gcttaatctt tgcaacatca
      421 tgtatgggtt ttagcgcaac aaccatctgt atggttgagc agttgggatt tgctattatt
      481 ccgttgtgcc agtcaacatc atcaggatta acctccggta caaccagcgg cacatccttt
      541 tccatccgcc atgctgaact gttgtcaata acaatacatc ctgagtcagc agccttacgt
      601 gcaacctcac ggcttatgga agctcctgct gaaaaaagtg ctatatcaaa ccccttgaat
      661 gcatcatctg ttactgcttc aacagttact tttttatcat caaaatctat tgttttacca
      721 acagttgttt tacctaaaaa acgaatagta ttgattggaa aattgcgttg caacaaaatt
      781 tttctaaatt caatccctac ggctcctgtg gcgcccagaa taacaacatt gtatttcttc
      841 atagtgaccc cccatcgaaa ttaacggtgt tttgtattgt tcatatatat cacttttttt
      901 tgtcaataat aacttttttt aaaataaaat tttatcccaa ttgacattta tttttttgat
      961 ggtattgtcg tcaaaagtaa ctatcgccca taaatttttt gttttacagt atacaatact
     1021 atgatagttt agtacagcaa aacattataa cgtcaatagc aacgaggtgt gtatgaaaaa
     1081 tgctttaatt gttggtgcta caggttttgg cggattaggg cttattgaaa ttttactacg
     1141 tcataacggt ttcaaaataa aacaacttat tgctcgcaag gatacgggta aaaaaatttc
     1201 agaggttttc cctcacatgg aaggcttttg tgatatgtat gttcaatcca ctgaagagat
     1261 tgattataat gatattgaca tagccttttt ttcaactcct gataaagccg gtatgcaact
     1321 tattcaatca ttttaccaga gaaatattcc cgtcattgat tttagcggtg atttcaggtt
     1381 taatactatt aatgattaca acatctatgc aaaaaataaa ggtatgaaca cagaacatgc
     1441 tgcacctgat attttaccgc atgctgttta tggattgccc gaaaaatacg ccgaaaaaat
     1501 taaaaaagca acaattgttg gcaatccggg ttgctttgca attgcaatga tactgggttt
     1561 gcttcctgca gttgaagaag gtattattga ttcagaaaca atcatttgtg atggtaaaac
     1621 aggtgtttca ggtgccggca aaaatcctgg tgaggctaat ttttatccac aacgatatga
     1681 ggatgtaaat acctatcgtg aagggtatca tcaacatatt gtagaagttg aaaacatagt
     1741 gaatgctcat gctaaaaaga aacatacaat tttttttgtt ccacaaattg tacccttgaa
     1801 ccggggtatt ttagttacca tttatgctga tgcgatagtt accatggata atgaaaagct
     1861 gtatgatgta tatgcccgct attataaagg caaaccattt attcatatca cacagcgttc
     1921 ctgccatact accgacgtgc gcggaaccaa cctgtgcctc atacgacctg ctatcgataa
     1981 aaggacagga aaactattca taacatcggt tatcgataat ctggtaaaag ggcaatcggg
     2041 caatgccgta caaaatgcaa atataatgat gggatttgat gaaacagaag gattaatggt
     2101 tcccgcattt tatccttaga tattattctt ttcctggaat catgggcaca aatcgtacat
     2161 aggttattac tttttgcgaa taggtattgc catgcctcac cattttaatt aattcctggt
     2221 tgtaatcacc cactggcgct acaaggatac cgccatcttc actgagctga tttatcaatg
     2281 catcaggtat tgttggtggt gctgcagtga gtattattac atcaaatggt gcatgctctg
     2341 gccagccttt atagccatca ccaattttga ctgttacatt gttataaccc aatgatttta
     2401 atcgtaacgc agcctgttta cccagtggtt caacaatttc aatagtatat acctcttttg
     2461 caagcaatga taatattgct gcctgatacc ctgaaccagt accaatttcc agaaccttct
     2521 ccgttccatc cagttcgcat aattcagtca taagggctac aatatatggt tgagatattg
     2581 tttggccata accaataggt aatggatggt cttcataagc agaagacaga cttagttctg
     2641 gaacaaattt atgacgctct acctttagca tagcctgtag tactcgttga tcagtgactc
     2701 cacgagcctc aatctgtttt ttaaccatta attcccgaag ctgctgcaat gataatcccc
     2761 tttcaccagt agaaatagca taaaatattg atataaaagc tatggctaat gtggctatta
     2821 taaaaacaat ggatatgatt tgtaaattca tttttttata actcataata acccatgtat
     2881 acatttatat tcatggaaaa cagcttttcc gtttgaataa taattaaaaa aactgtaaaa
     2941 tatcaattgt aaccattgga ttttccatac tgatacaaaa aattttaatt ttaactatca
     3001 cgttataata aatataaaat ttattaatta tcttgacaac ttttttacat tacttattta
     3061 attctttttc tgaaacagtg taaaatttga tttacattaa tatagaatat agcagtatac
     3121 ccttatttag tgttttaatg gtgctgagaa ttattatggg cgatagtttt tttacattaa
     3181 tctttttata gtaattacag gtattagtta ttatgtcaaa atcaattgaa ggggttattg
     3241 cctcgccggg tattgttata ggtaaagcct atatatatag aggggataat atcattattc
     3301 caaaatatac aatacctgcc gaacaggtag aaaaagaaat taaacgatat gatgaatcag
     3361 ttaaaaaaac aaaagaagaa ataaaagcaa tccaatcaca aattgcaaca agcctttcca
     3421 gtgatatggc tgatattttt caaagccatc ttatggttct tgaagatccc tatatggatc
     3481 aaaaagttcg tgaaacaatc agcaaagaaa aacgaaatgc cgaatgggtt ttaaatgatt
     3541 tttcattaca attaattcaa aatttaaaat ccatagaaga tgagtatcta cgtgaacgca
     3601 tcattgatat tgctgatatc aataagcgct tgataaataa tttacaaaaa acacatgatg
     3661 aatcattagc cgatttaaaa gaggaggtaa tcattatagc aactgattta acaccgtccg
     3721 atactgcaat gatgaataaa aagtatgtgt taggctttgt aactgaccgg ggaggtagaa
     3781 catcccatac tgcaatcatg gcgcgtgcat tggaaatacc tgcaattgtt ggtgcacagg
     3841 ttgctacttc actcattaat catggtgata tgataatatt ggatgcaatc catgggaaaa
     3901 taattgttaa tccatcaaaa gatgaaatca gtgaatatga aaaataccgt gatgatctta
     3961 aacaactgga aaatgaatta gctacaatca tagaacttcc ttcacatacc ttagataatg
     4021 agacagtata catttatggc aatattgaaa taccagaaga aatgaacata atccgcgacc
     4081 atggcgcaca gggtattggt ttattccgat cagaattttt atttttagat aaatcgttgc
     4141 ctgatgaaga aaggcagttt gaagaataca agcgagtagt tgaatttttt accccacatc
     4201 ctgttaccat acgtaccctt gatattggtg gggataaaat atatgcattt accaaacaat
     4261 ataaagaacg gaatcccttc ttaggttgcc gtgccattcg ttttagtctt gaaaatgaat
     4321 ttttgtttag accgcaattg cgtgccatac tcagggtttc tgctctgggg aatgtcaaat
     4381 tgatgttccc aatgatttca tctgtggaag agtttttaaa agcaaaatca attttacatg
     4441 aagagatgga aaatcttgat aaagccggca tcccatttga taaaaatttg ccggtgggaa
     4501 taatgataga ggttccctcg gcagctatta atatcgatat attttgtaaa tattgtgatt
     4561 tcttctctgt tggtaccaat gatctggttc agtatacatt ggctgtagac cgaatcagcg
     4621 aaaaaattgc ttatctttac aatcctttaa atcttgctgt tctccgtttg cttaagcaga
     4681 tagcagatgt ttctaatcaa aataatatac cgctatctat atgtggcgaa atggcaggtg
     4741 aaccaaaata taccatgctc cttattggat tgggatatcg acagttgtcg atgagttctg
     4801 cttttatgta tcaaataaaa cgtatcatac gttcagtaag cgttaaggaa tgcattgaaa
     4861 tgactgatta tcttttgcag cttcacaaca cctacgacat tgaaaatgct ataatggaag
     4921 ttttgaataa aaaattcccc aatatgtttt tttaatgatt aatcaaatta caatcataaa
     4981 actatgaaat tatatcatga tttagctgaa tattattttg taattgaaaa agggaataga
     5041 aatattaacg atgatgtaaa tcttattctg gaactatcga acacaaaaaa aaatctttta
     5101 ctccttgatc ttggttgtgg tacaggtgaa caccttaatg aattatctaa aaaaggtttt
     5161 atctgtacag gattggatta tagtaaaaat atgttaaccc acgcacaatt acgatttccc
     5221 aatgctgcaa actttttact atgcgatatc accaactttg acttttatga ggaatttgat
     5281 gtcattattt ctttgtttgg ttcttttgac tatatgattg acaatgatga tgttgataaa
     5341 gcattatgga atacatggcg tgcaatgaaa tcaggtggat ttggactctt tgaaatatgg
     5401 aatgcaatac ctgtaagaaa gataaaagaa aaaccattga caacagtatc aaaagttaaa
     5461 tataaaaaca tgacaattga acggcaacgt ggttttacac ttatccctga tgacaataaa
     5521 acaatcaccg aggttaatta tatctacact atatctgaaa attcaaaaag agaggttata
     5581 aaagataaac atatcatgcg tgctttctat aaggaagaaa ttgaaagttt tttcgctaat
     5641 aacggattaa gcataatcaa tatctatgca aattcaaaaa aagaaacatt taattcacta
     5701 tccaataaaa tattgattca ttttatgaaa gaataaaatg tataagctat gtataattat
     5761 gttttgtcaa taattcatta gtaacatctg aaattacact ttcccttaca gtatctttcc
     5821 attgttcagt aagtttttct gttttctgtc tatccttaaa aaatttatca ggctcttcta
     5881 atgaaaggaa gagtacctct tccatactgg taacaggatg aaagataagc tgcttgcgaa
     5941 catattctgg aattttttca agatcttttt cattatccat tggtataatt aagtgtttta
     6001 tctgagctct atgtgctgct aaaacttttt cctttaaacc acctatgggc aatacttttc
     6061 cacgcagtgt aatctcacca gtcattgcaa catcacatct caccgcaatc tgactgagta
     6121 acgaagcaat aacaacaccc attgttatgc ctgctgaagg tccatctttg gggatagcgc
     6181 cttctggtac atgtagatgg atatcatgtt ttcgtaatac atcttcgggt atattgaaat
     6241 actgtgagtt tgcccgtaca taggtaaatg cagcatgcgc tgattcctgc ataacttcac
     6301 caagctgacc ggttaagata agctgacctt tgcctttaat taaagatgct tcaacctgta
     6361 aaacatcacc tccaacctct gtccatgcca acccattggc aagtcctatt tcactctttt
     6421 gttccttttc tccatattta aacttaattg ggcctaaata ttcatgaaga atatcaacag
     6481 taattttcct gatatgcaaa ggaccgtttt ttactacttc acgagcaact tttcggcaaa
     6541 cttttgctat aacacgctca agatttcgta cacccgcttc acgtgtataa tgtcgtatgg
     6601 tatagagcat tgcatcatta ccgtattgta tatgtgattc atttaatcca ttttctttaa
     6661 tttgctttgg caataaaaat tgtaatgcta tatttaactt ttcaggctca gtatagctgt
     6721 ttatttcaat gatttccatc ctgtcctgaa gtggagcagg tattttatgc tgtacattgg
     6781 ctgtagtaat aaacagaaca tcagataaat cgtacggaac ttccagataa tgatcaacaa
     6841 acgtattgtt ttgttcggga tctaaaactt ccaataacgc tgatgaagga tcacccctga
     6901 aatccattga catcttatca acctcatcaa gcaaaaatac gggatttacc acacctgctt
     6961 ttttcatctg ttgaattatt ctgcctggta atgctccaac ataggtgcgt cgatgaccac
     7021 gaatttctgc ttcatcccga actccaccca atgacatacg cacaaacctt cttcctaaag
     7081 ctcgtgctac tgaccttccc agcgatgttt tcccaactcc cggtggccca acaaaacata
     7141 gtatgggtcc tttcaattta tttgataaat gacgtacagc aatatattct ataatccttt
     7201 ctttaacctc ttccagccca taatgatctt cattgaggac atgcattgca tgatctatat
     7261 ctctattatc acgggttttt ttgaaccaag gcacatcacg taaccattca atatatgttc
     7321 ggacaaccgc tgactctgct gacaaaggag gcatcttctc taaacgtttt aattctttca
     7381 gtgctttgtc ttttgcctct ttagacatct ttgcttcttc aatagattta tgtaattctt
     7441 caacttcatc agctaaatct tcaccctgcc ctaattcttt ttttataact ttcatttgct
     7501 cattgagata gtactctttc tgagtttttt ccatctgttt gcgaacttta ccctgtatct
     7561 tcttttcaat atacattatc tcaatttcgc catttataag ctcaatgagt ttttctattc
     7621 tttcaattgc gttacctaat tctaaaattg cctgtttaaa ttctactcgc attgaaatgt
     7681 acgaggcaat cacatcagct aaatgagaaa tatcttcaat ttgcatcact gtagttaaag
     7741 cttcttgtgg tactttttta ttaagcttta tatacttttc aaatgtatca ataagatttc
     7801 gttttaatgc ttctactttt gaatctgctt catatggtcg ctcaatgttt ttaattgaaa
     7861 cggtaatacc atcatcatct gtttcaaact gtgaaacata aactctgtca attccttcaa
     7921 ctaacacttt aaccgtacca tcaggcagtt ttaaaagttg taaaatttca gacaaagtcc
     7981 caacatcatg cacatcttct ttttttggat tggcaatctg ggaatctacc tgggcaacaa
     8041 gaacaagcaa tctgtcgccc ttcattgctt tatccagagc ttttattgaa atatccctgc
     8101 caacaaataa cggaataacc atatgcggaa atataaccat atccctcagc ggtaataaag
     8161 gatatctttt aaagaaatct atatcttttt tcatagtttc tccctataag gataataagc
     8221 tttcaattaa taaaatacta aaaaaattct cttttgaaaa cataactaag cacttttttc
     8281 tgtagttcgt tgaataatat caggaggttt acgatgcaaa acaacatcat cattaataac
     8341 tactttggcg atagtttttt tcgatggaat ctcatacata agatctaaca tgatttcttc
     8401 tagtactgat ctcaatcccc ttgcaccagt ttcacgttca attgctattt gagcaattgc
     8461 ttcaattgcc tcactggtaa attcaagctc aacattttca aatccaaata ttttttgata
     8521 ctgttttgta agagcatttc gaggttttgt taaaatatcc cgtaaagtat gtttatcaag
     8581 ttcttgtaac actgcattaa catgtaatct accaacaaat tcaggaataa gcccatattt
     8641 aatgagatca tcgggtatca tcttttcaag tatattatcc ttgccaatgt ccttgactga
     8701 tttcacttca gcaccaaatc ccatcgtttt attgccaatc cgcgcatgga taattttttc
     8761 caaaccaaca aatgcaccgc cacatataaa taaaatattc ttagtatcaa ttgatataaa
     8821 ctcctgatgg gggtgctttc tgccaccttg aggaggaaca ttggcgatag ttccttcaac
     8881 aatttttaac agtgcttgtt gaacgccttc gccggaaaca tcccttgtaa tggatggatt
     8941 ttcggacttt cgtgaaattt tatcaatttc atcaatataa attataccaa tctctgcccg
     9001 ctttatatca ccatcagcat tttgaattaa gcgtaataat atattttcaa catcctcacc
     9061 cacatatcct gcttcggtta atgatgttgc atcagcaatg gcaaagggaa cttttaaaat
     9121 ttttgctaat gtctgagcaa gcaatgtttt tccacttcct gttgggccaa ttaagaggat
     9181 attgctttta tctaattcaa catcaccttt acgcaggtta aagttgctgg taatcctttt
     9241 ataatgatta tacaccgcca cagagagaac tttcttggca tattcctgac ctataacata
     9301 ttcatcaagg attgctttaa tttcatgcgg cttgggcaaa ttgtttgtta aaagatcccc
     9361 cccatcaaga gcccaatctt cctccaaaat ttcattgcaa agctctatac actcgtcaca
     9421 gatatagaca tttggccctg caactaactt tctcactaaa tcctgacttt taccacaaaa
     9481 cgaacaataa tgaataactt ctggttttgt attatctttt ttcttattag ccataaatcc
     9541 tcatgttcaa tatactatat acaatactta tctactatct tttcgtataa ctttatcaat
     9601 taatccatac tcaacagctt cctgtgcatc cataaaatag tccctatcaa catctttttc
     9661 aattttttct aaaggctgtt ttgtatgaat agtataaatt tcatttaact tttccttaat
     9721 cttcaaaatt tctttcgcat gtatagcaat atcagatgcc tgtccctgaa acccacccat
     9781 aggttgatga atcattatac gcgaattagg caatgccgaa cgctttcctt gagcacctgc
     9841 tgctaaaagt aacgatgcca ttgaagcagc ctgacctaaa caaatcgttg caacatcagg
     9901 ttttacatat tgcattgtat cgtagatagc caatccagat gtaacaattc cacctggcga
     9961 atttaagtat agatagatat ccttatcagg gtcctcagct tccagaaata acatctgtgc
    10021 aataaccaga cttgctatat gatcatcgat tgcatcgcca ataaaaataa ttcgatcctt
    10081 caataatcgc gaatatatat catatgcacg ctcaccccgt gctgtttgtt ctactacaat
    10141 tggtacaaga tagctcatag ggttacctca ttattatcat ttttttggat atgttataag
    10201 tatagtttag catttcttta ttgctattca ataaattatt attgttcagc aactgcttat
    10261 ttttttccat ttacatattc ttgaaatgta ataggttttg acatcttgat atctgcatgc
    10321 tcatatataa aatcatatgc ggaatcaaga atcaaatcac cttcaatctc ttctttatta
    10381 cctttttctt ctactgtttt gacaacctct tcttcagaaa cagtattacg ctcagcaaca
    10441 gattttacaa atttttgaaa ttgttcctca gacactttta agttctccaa ttctgctatt
    10501 ttgctcaatg ccatcgcatt tttaatgttt ttatcagctt catccataat cgatttggat
    10561 agctcttccg gattaattcc aaatatagaa gcaaactcat ttatatcatt gccaacattt
    10621 ccaaatcgtg actgcaagcg tctaaaaatt gcatacattt ctttctgtat aagagattga
    10681 ggaatatcaa atttgctgtt ttcaactatc atctctaata ttttattctt cgtttcattt
    10741 aatgtttgtg actttacata cgtctcaaga ttttcacgaa tacggttttt taaatcatca
    10801 accgaattat attcaccaac gcttttagca aaatcatcat tgagttcagg taatttaaat
    10861 tcaaaaattt cctcaacttt taccaggtaa gttacttttt gaccttttaa gtcttccacc
    10921 tcataatctt tagggtattt aacttttatc tctttttctt catctttttc catccctatt
    10981 atatcatcat caatagccca tgattccttg cttttcccaa ctattaaatt atgcgaggta
    11041 aatgcagcag catcaatttc ttcttttgac ttatcatcaa tacgtttcat tttcaaagtg
    11101 acttgatcgc ccttttgcac tttgatagta tcaccttctt ttttggtaat ggttgccatc
    11161 ttttcacgca tcgtttcaat ctcggcttca acgtcttcat cggttacttt tactaccttc
    11221 tgttcaacat ggcttccttt atattgacct aattcaatag taggaggaat atcaaattca
    11281 attttgaagg aaaagggctt tcctttttca accttttcaa aatcaaaaac tggctggctt
    11341 actggcgtat agcccttctg ctcaattgtt tcaacataag ctgaacgaac aatattttcc
    11401 gaagtttctc ctaaaacgta atcagaatat tttttttcta ccagatcaat aggcgcttta
    11461 ccaactctga atcctttaat ttcagctttt ttttgcaact cttttagtac tcttgcatat
    11521 tctgcatcaa tacgatcacc tggaacttca atatcaagac gtattcgagc attatcaagt
    11581 ttaaactctt taatttccaa ggttaactct cctaatatat gtaattttat ttaaaattaa
    11641 ggctttatat gtgcttgata aatattataa aaaatactcc tcatagtgta taaacaaaac
    11701 gtaaacgtta atgattatat atgactataa atatagcaca ttgatgcatt tataagcgat
    11761 attgtaacta ttaatgatat attttattct gtaatgtcag gcgtcaaata ttttttaata
    11821 ataattattc ggcgcatata aattatttgg gcgcaacaca ttcttgattt ttcccaaaag
    11881 cttttgagcc attactcctg acagggatga tagtagagtg acgcatggtt atatttttaa
    11941 gcacataaat atccatcata tcaattcttt tagcaaagcg atagttattg ttaatgaagt
    12001 cgtagagtgc atgaaaattt tttagcggga atttatcata gccgtaataa cccgaaggtg
    12061 aagtatcaat aacgacagca ggaggatttt tcataacttc agcaataaac tgctttctca
    12121 gaagttgaat atacgcattt acatttgcct gcatatcttc atttgatact ccaaactgtc
    12181 gccccgaaat atagtctgca ataatgattt ttgatgcgca tcgtctattg gcatagatat
    12241 acgcaacagc accggaaccc catacaaata tggtatcatc aggagcggta atttcagtta
    12301 ctgcatgtgc aacattttta tacgcataat ctgataatga attactatcg cccagataat
    12361 ggtatgttaa tggaatattt atccttgatg ctgtaaatag tatcatcata ataataaaat
    12421 atatatatat tttttttaac aacgatagtg ttatatcctt aattaataaa cctcctaata
    12481 ttgcaagtgg tggaaccaat tgaataaagt aatgcccata cgccctccat cccaaaagta
    12541 ccgaaaaaca tccagccaac agccatagta tacttataag agtgattggc gtatattgct
    12601 tttctttcaa taaatgaatt gcatgcctgc ctgctatgag ccatagaggt aaactgcata
    12661 ctataaaaat aataaaccgt gctattgctt ttactatggg tacagttgtc attccgctct
    12721 gtatatattt aaaattatat aaaaaaccca atgtaataaa atcatccaat gcattgttac
    12781 aatacaaata gcttgttgcc ccagccacta ccattaaaaa tccagtagca ataacaatca
    12841 tatcttttag ctgtttaaca aactctcttt ttttattggt tgcttttgtt atactaaaaa
    12901 ttgcaaaaat accataaaca attaaaataa ccccagcttt ataattaata aaaaaagata
    12961 ttccggcaaa aataccagat gtaactaaag ctatgtttgt cttccactta ttgataataa
    13021 aaagatagta acttataaat gaaataagta ttggtaatgt aaacagtgtc tcagtattgg
    13081 ttgcgatata ctttggttca tatatatgca ttaaaaaaat aaaaaacaag ctggctgctt
    13141 tagcctcatc tattcctcta atgttttgta taaacttata gataatgatt gcacagcaat
    13201 agacaagtat aatggtaaca gcatggacag caaaaagatt gttgattcca aaaaaataga
    13261 aaactatgta ataaaaaaaa taaactaaca atggtttatt atcatacatg tgtacatagg
    13321 gaattccacc attaatccat ttattggcaa attctgcaaa agcagcttca tcaatatcaa
    13381 ctattgtatg atacaatgct ggaatacgga gaacaaatac cagtaaaaac aaaaaagtat
    13441 aaaaatatac attgtttcta tttactattt ttttatccat atcctgcttt tcccaaaata
    13501 accgttaaat gtataatgtt cattaagata gcgtatgatt gctggaaaat catctaaact
    13561 ggcaaaatgt ccaaaatgaa ttattggcgt tgcatgaata ttaaagtaaa ataaaggatc
    13621 accttttttt ctatatacca ttaccttctt tatcatatca gtagaaccca catctacaaa
    13681 aagatcaggt ttatattgga taattaattg tataatataa ttttcatatt catgtatata
    13741 gtgttcattc cccttctctc tttgaaccac tgcatacaga gccctttcat tcaaccagta
    13801 tgaagggata acggattcgc ccgtagtaaa ataatcaatt tcagtaccca ttggccagat
    13861 taacattgtg ctgccaggat gatttttatt aatatattca atagcacgca ataaactttt
    13921 atgcggcaac aaatattcac cgtacgaacc aataatagca attctgaata tctcggttaa
    13981 cctactttca ttatgcttta aattgggatt gcatgtataa atatagagat ctttgtaatg
    14041 ccatgcaaag aaaaatacaa tggtaaacat cagttgcata aataccatgc gtttccctct
    14101 ttcagaaaac acataaagta tttgctttga agaaaggatt gcaatagatg gcattatagg
    14161 gataaaataa tgaactgata aacgacctaa accagaaaaa acaaggataa aaaagcatac
    14221 tgaaacatat agtataaata atgttttgta atctattttt ttatttttaa aatcatatat
    14281 aaatttagct attaggataa tgccgggaaa ccagaaaaaa gcatgaaaaa ttgaaaattg
    14341 atatactctc cacaacagct taatcaaaat tgagaaaata ttaaaacctc tattagcaac
    14401 ataggtatac ttgcccatta ttgctttgta gatataatca ataaaatcaa aaccaaaata
    14461 tacatatcca gcaattaaaa aggttgcaac ggtaaccaat gcactagcaa tggcaatgaa
    14521 aagatttttg taaaaacgtc tgtaatcatt ggtatttaat gataatggat atataaatat
    14581 aaaatgtgtt aaaatgatag gcaggataaa gattccctgg aatttaatca aatacgatgc
    14641 aacgatattg attacaagta atacaatata tattcttttt ttttcaaata cacatacata
    14701 gtaacaatac agtgcaaaaa cgttaaacaa attataaaat atttctccat taaatgcaag
    14761 gtactcagta tgatagcagg atataactgt gccatataag aatgctgcaa ataaccccgt
    14821 tttagaatca tacatttttt ttgctaaaat ataaatgcca tacattgtcg ttaaaataaa
    14881 aagcaatcca ataaaatgaa acgatgatgg tgtatcacca atatagttaa tagaaaattt
    14941 cataagctga tgatacaacc atcccttatt catgctgtat gatagtccca ataaatcacg
    15001 ctttgccagt aaatatgatg ttaaaacatc aacatgaata aaatcaataa tgaccgacgg
    15061 taaccgtaag attattgtac tgaagcagat acatacaaat accatgaagg agtttttgtt
    15121 aaataattta taaaaaattt taacggtatt gtttagaaac aatgcatttc tccattgaca
    15181 tgattacata ttatgccaaa atgatgttaa atttttaaat gtaccgtcaa cataattttt
    15241 tttatgtatc caataaaatt gtattgtaaa aaacacagtg cattaattta tactctccat
    15301 ggtgcaatat attatcaagt ttgtcatatt ctttgtaagg tataatagca atattatgct
    15361 atgcattatt tattgaaagg aattgtatat ggataattgc atttcaatca tagtacctac
    15421 gtataaagaa gcagataata tctctgttct tgtagaatct attgataaag caatgagatc
    15481 agcaaatctt aactatgaga taataattgt tgatgataat tcaaatgatg gcattgtaga
    15541 aaaagttaaa caattgaaca aacaaaaata tccagttgcc attaaagtaa gagttcatga
    15601 acgcggttta tcttcagcag ttatagaagg atttaaatta gctaaaggaa atatatatct
    15661 ggttatggat gccgatttaa gccatcctcc tgaaaaaata cctgatatcg ttcaacctat
    15721 tatcagcggc aaagctgaat ttgttgtagg aagcagattt gtagaaggcg gttcagcaga
    15781 acattttaat atatatcgta aactcaatgc atggatttca aagatgttag ccatgccttt
    15841 tacaaaggtt aaagatccaa tggcaggttt ctttgcgttt gatgccaggc tattaacacc
    15901 agagatactt ccaatcctta accctcttgg ttttaaaatt ggactggaga tgatagttaa
    15961 gtgcaaacca aaaacggttg tagaaattcc aataacgttt aaggaacgtt tatatggtaa
    16021 aagtaagcta tcaacccgcg agcaggtttt gtatttactg catttaaaac ggctattcca
    16081 ttataaatat ccatcgatgt atcaattttt atttttttca ataattggcg catcaggtat
    16141 gatagttgat ttaacaactg tctttattac ttatgaagtt ttacatataa aatttatcat
    16201 tgcccgtgcc attggcttta taatagcgtt aacatggaat ttctttttaa atagaaaatt
    16261 aaccttcccc gattctcctc aaaaaaatat ttttaagcaa tacagaaact tttttcttgt
    16321 atgcagtata ggtggtttgt ttaactggtt tatttcggta tttttatact ggcactattc
    16381 atactttaac caattttatt tacttgctgc ttttataggg attttaggag gattattgat
    16441 aaacttcttt ggttcaaagt ttattgtttt tggtaaataa tatctaatct ttgttggcta
    16501 tcatttcggc aatcattcct acagtaagta tgtttaagcc tgatagtgtc aaaagtactg
    16561 aaatagtgtg gatattctga tacagaattg cctgcaaaag cgttagtata atgccacccc
    16621 caaaaagagg aaggctaata ggcaaaaata ttttaagcgg atcaaaaaac attacagtac
    16681 gtataataag cctgaaaaag tttaacgtat cataaaaagg tctaatcttt gatttccctt
    16741 tcctatggtt atagttaatg ggaatatatt ttacatgaca tccattagat agcatagcta
    16801 aagtgatagt tgatgtaaaa gaaaatccat caggaagaat cttaaaatat ttttttacaa
    16861 catccttctt cattatccgc atacccgaat ttaaatcagg tattttagtc ccaaccatat
    16921 aatttgccat tttattaata acccatttgg gtaattttcg aataaatgga atgtgaactg
    16981 actcgcctat tcttgctcct actaacatgt ccaatccttc attgatgaca atttcaaaaa
    17041 attcaggaat gcgttcatct ggatatgttc catccgcatc agtaatagct acgtattgat
    17101 gttttgaagc acgtattccc gtctttaatg cagcaccata accttgattt tcagaatgat
    17161 taattacttt tatatcctta ttgttaggca tccttgctaa tattttttcg gttccatctg
    17221 tagagccgtc attaacaatg ataatctcca tatcaatatt tttgcatttc ttttttattt
    17281 cctttaaatg attaatggaa tgttctattg cactaatctc attatacact ggaactataa
    17341 ttgttaatcc tgatttcatt gaatcctctc ttacatagta tgtattaatt taattataaa
    17401 aaatgaattg ttatcttttt ccatgtcaag ataattatta caaagtatga ttctttatca
    17461 tgtaaatgta atggtatact tagtttactt ttactttcat ccttttcttg atatgatttc
    17521 taatcagcat aataagattt acaagccagc catgaaatcc aaggataggg cttaatgcaa
    17581 agattgatag tgaaaacaac actgcacttt ccctgcccag accaatgcta ttaaaaagaa
    17641 aaatcattac agcttctcta acacccaaac ctgaaacaga tattggcacc gctacagaaa
    17701 aactggaaac agcaaggcaa aatatattgt atgtaaatgt catatttatc ccaaaacttt
    17761 tcaatataaa atatccaacc atacaataac ataaaaaact tggtattgtt ataactatac
    17821 aaattgcaag atattttact gttgttacca tcttaataat atcatttata aagcctgaaa
    17881 tattattttc gatagttgca tagtattttt ttggaactaa aaatttacta aatgtgatga
    17941 taatattttg aattgattta tatttagcta acacaacgat tccagctata atacaggata
    18001 gtaaaataat aacatcaaca tatttaaaat gaaagaggtt actataaaaa ggatacagta
    18061 taaaaatcat tgcaatacga atgcctacat catacaacct atcaacaaca accggggaca
    18121 acgaactccc aaaattgtca aacttttcct gataataaag tgctttcgat atttcaccta
    18181 tccttcccgg agtaacagca ccaaaataaa atgcccatag atatacataa aaggattcaa
    18241 atatggaatt acgtacacct tgcgttttca ttaaaaaatt ccatcgtaca gctttaagaa
    18301 aaatgttcat taaaataaat ggataagcaa gaagcaaata ccagtaattt gcatctttta
    18361 aatacttaaa aacttgctta aaatctattt tccacaaaat gattgctaaa aggattattc
    18421 ctattccatt aagataatta atttttttac tcaattcatt tacctattca tgttcagtat
    18481 ttttaataaa gtattaatat tttaatttcg caaattggtc acaatatatt gatacaattc
    18541 tgaaggcaac aaataatatg aaataatcga taccatatta taattataat cacaaaaatt
    18601 atctaaatat ccttttggtg gaataatgct tcttttgctg tatgaactat atgtaacatc
    18661 aatatcatca tggatagtaa ctattttttc tattctcatc agatgtaatg gagaagataa
    18721 taaaattatt tttttaaaat ttttaacaac tataattttc tcagcttctt tccaattttg
    18781 cactgtatcc cttgaatgtg tttcataata aatatgactt ctgtcaactc ccatttcctc
    18841 tgccattgta gccattaatt tagaaccgga agtatttatt gaaggccgcc atccgccaca
    18901 gaaaattata ttgttaattg catgttgttt gtataattca attgcaaaag ataagcgcct
    18961 caatgattca tcatcgattg tattatgagt ttcaaaatca ctaaaaaaaa ctacagcagc
    19021 atcaaatgga gtattgatag tattgtatag tatatcatac tttttttcag cataaaaatc
    19081 tattatcaca aagattgcaa aatacacaag aaaacaaacc agtaaatata gtgtaaactt
    19141 tttcaatgtt attgctattt atctccctta ccaagaataa aagtccttcg caaggcaata
    19201 attcttttaa tgaactctgg gagatagttt tctaaaaatg tattgattgc tatggatagt
    19261 ttattattga agggaaggat aatagtactg ttttcttcaa gaatttccat gcgatttttc
    19321 tttatgcatt gaatcaactc gcctcttttc aaaaaacgat gtggaccttc actatgattt
    19381 atattgattg ctgctgaaag ccaatgaacc cattcccaca tacgattggg gcaggtcaaa
    19441 atcattattc catctttact catgattcgc acaagttcat caaaaaaggc agagtaatca
    19501 taaatatgtt caatagtctc atacgaacaa accagatcaa attgtccatc attaaacggt
    19561 aatggaaaat taagaacttt aacaatagaa tattgtaatc ctgttttgtt caatcgttta
    19621 tcagcaagag aaattaaaaa atcagaaaaa tcaacacata cagagcttgt gaccttcccc
    19681 ttttcatgcc agaataaact gcctttgcca cttcgagcct ggatatctat catattgtat
    19741 gtatcttttt ttagatgttt atatgcaagc tcataagaat tggtaaatct tctatgatac
    19801 gtatacagtt gttcattgac agcatcataa tcattgacag tatcccaaaa ttctccaacc
    19861 tgttttaaag tccatggccg cctggtgtaa tagtcatatt cctgtttgac tcgtttccag
    19921 aatcctttaa gggtaaagta atattgctca ctcataatta ttccttatca agtagctttt
    19981 gtttttaaga aaactatcgc acacaaaaat ataaaaatat tttttgaaat gtccattata
    20041 aaattgttaa acatttgact tttcattata ctgcaaaacc ctgtccctgg cattgggttt
    20101 atatcccatt aactttttaa aattttttat taaaaaaaga ttaaaattta aaggcataat
    20161 acttaaaaag aatttaaaaa cactggttga accaagaatc aatataatat tatatataaa
    20221 atgatacaga tatcgatcaa gtttttctgg ttttgcaagc ccaatgaatt caggtttctt
    20281 tttggcaaac tgcaacctta caaaaattcc attgagctta tatgagatat taaatgggaa
    20341 tgattcatga atttctggag gctctatttc ttctaaatag agagcatgag ccttttgagc
    20401 tgcctctaaa agagaaagtc ccttctgtga ccttgcaatg atgcttgacc aacctttggg
    20461 tatacctttt tcattcatgc ctcgccctaa aagtcgtaaa agccatgaat cgccaaagct
    20521 gatatctgaa tagttgccat aatagtcagg gcataacctg cacctgtttg gcaggtacac
    20581 ggcatttaaa tagtgatagt taaaaaaatt gatacctttg cgttcaccat ttttaagata
    20641 aaaagaaagt ccaccgggcc aatcaccata atccctatac tttatatcaa cgacatcctc
    20701 ttttttaata ttaagttttt tcatgccgaa tgtcgttgca ccgtactcca tattatgccc
    20761 acaaatcaac gataatatat attcaatttt ttgagcgttt ttattatgct cgtgctgaag
    20821 ttttcttaaa ctatgtatct ggcatggaag agcaacgatt gcatatcttc cttttttccc
    20881 ttttagcgtt tttaaaattt cattggttgg tataaggcaa tactttgatt gagcagatgc
    20941 tttaacagtc tctacattag taattatttc aggtttaaac aaccatggct gatcagcttt
    21001 ttgacgggga agtattactc cgtcaaccac tcctgcctcc agcatataca ccaataatcg
    21061 ggtaacgcaa ccaccactgg ctccttgtat tcttatcata tcatccagtg aatgtcccaa
    21121 aaaacacttt ttataacttc ccaggtagtt gtcatatctt tcacctaatc taaaatgatt
    21181 atcaagttcg ctaaaattaa cacctgcacc ctgacaatta ttataacata agctacaggt
    21241 agtacattta gatatatcaa acaccggacg ccctttttca taatctgcag aaagggcatt
    21301 ggaagcacat acccctatac aggtaccaca aaaattacaa agcggcattg ctttgccacc
    21361 cagtttttcc atgagatcat taaagtcgta ttttactttc aatggcatta aaggaatact
    21421 cctttctgtg tttttgtttt aatcaataga aaagcgcaat gaaaataaga aattaaattt
    21481 gttgttgctt ctaaatttaa ttcagctaca ataaagtcaa ctaataaatt cttaatcaat
    21541 gattgtccct tatttaaaac catttacagt tgtaattgga ggaatctttt catactcaat
    21601 attataaacc tttctcaaat gattcgtata ttgttttgaa aatatactaa gacttgcatc
    21661 aaaatcagta aattttaatt ttgataaatc tgcgatacta tatataacat tttcagtgct
    21721 atatgctcta tttagatatt ttgggatgtc ttcaaaaaaa cttttattag catttttgta
    21781 ctcatttgaa acccatacaa taaatggtat ttcacacata tactttgaga agtttctact
    21841 attatgcccg aacatatttc tgaattcaaa aagttcttca ccatgatcag aaaagtaaac
    21901 tacccatgca tatttacagt gagattttac acgtttaata attgagtaaa taatataatc
    21961 attaaacctt acactattat catattcatc aatgataagt ttttgtttat catctaatcc
    22021 ataatcatat gggataggga tttttttatg attaaaataa ttaaagatat cggaataccg
    22081 tgaactatat tttgtatggc atcccctcag atgaattatt ataaacaaat ttttatgtgg
    22141 ttcatcagta agcactgtat caagtttatt tataacaact tcatcggttt ctgatgaaac
    22201 gtcataatat tggttgcatt ctcgagcaac aatgctatgg ctaacattat ttctgttacc
    22261 ccgcaaactt tgatttccta tccatatagt ttcaaatccc acatcattaa aaagatttac
    22321 cacatttctc ttttcaataa aatattcggg atgatctaaa ttagcaaaac ttaaaacata
    22381 tttcataaca tctactgttg ttacagctgg tgatatgacg ttattaaaaa tatataattc
    22441 atctttaatt tttattaatt ggggatttgt ttctcgttgg tatccatata tctgcatgtg
    22501 tgctcgtgaa agcgattcac ctattactaa gacatataac ttatgttctc gtatttttaa
    22561 atcacatgtc accttatctt tccatttaag gtcagccctc ttggctttta ttatttcata
    22621 atttttatta ttgataaact gataatatga tttataaaaa tcaactacat gatattcctt
    22681 ttgtaataat ttccaattca ctgtgaaaat caaaatcaaa accaagaaag aacccattac
    22741 aatcaatttt ctcttattat tatttaattc catctgtata tctttcattt taaaaaacaa
    22801 tggcataggg ccaaaatatg ctataagtaa catgataatc ttaaaattga caaactgatg
    22861 aataaattct aatgattcat ctaaatccgt tgtgaaaata acatcaaaat cacttctatg
    22921 ataaattgtg ttagttatag aaaaagcaga aattataaaa aatgatggaa ttattgaaat
    22981 tatcaataaa ccaattataa atatcctgat tttcttattc gaataaacat agatggcata
    23041 taaaagtaaa gctatgaaaa acaataaaat taataaattc aaagaaatgt taacagggat
    23101 atatttatca aaaaaataca aggcaacagg gaaaattaat atccaaaaaa ttattataaa
    23161 atacttcacc ttatatttac ccatcattac gttttacctc actttattca aagttaaaga
    23221 attgcatatc aaaactatat agtataaaaa tttttcagaa taacaatatt tttgttttca
    23281 taattacctt atacatatcg tcaagcaatt tacatcaaat agtttacctt attttccatg
    23341 tcattgatat gtggattctt caaatagaat ttaaaatata attgactttt cttatattga
    23401 atactttcgt taagtaacaa atcaatttta gaatgtaaaa cattaacaat tatttaatcc
    23461 atatcataag gattcgttat gctaacacct gaagaacttg aactctacca gcgtcagctc
    23521 attatcaaag gctggggcaa agaatctcaa gaaaaaataa agaaaaccca catctttatt
    23581 gcaggagctg gtgggcttgc aagtaccgca tcattatact gtgcagtagc cgggtttggt
    23641 acaatcacac tctgcgatta tgacaccatt atacactcat agaaaaagca cacatcattc
    23701 tggactgcct ggataacttt gaaacacgcc atattctcaa caaggcatgc gtacaacaaa
    23761 acaagccatt aatccacgcc ggagtggaag gattcagcgg ccagcttaca gtcatacttc
    23821 caggccaaac tccatgctta tcgtgttttc taccaaagaa aagcatcaag aagccaattc
    23881 cagtagctgg cgttacacct tgtattatgg gcacacttca agcccttgaa gcaattaaac
    23941 ttgtaacagg attaggcacc attacagcaa atcagcttct tttatttgat tgcctttcca
    24001 tgcacttttc caaaataaca ttataaaaga acccacattc taaaatctgt aagcaataaa
    24061 aaggccgctt atgtaaagca gcctttttat tttagtatga gcgatagtta ctgtacagta
    24121 actatcgata acttacttac tctttgcgtt tttaatgttc aactgagcta tcgctaactt
    24181 tgacaatggt attgcatacg gtgagcagga aacatagttc aaaccaacat tcatacaaaa
    24241 ttcaatatta tccgggtcag ctccatgttc accgcaaagc cccatggtga tatctggacg
    24301 agttaggctt ccacgagttg aagccatttc aataagctct ttaacttcac gccccaatac
    24361 cttaaacgga ttattgggaa tcaggtcata caacgaatag cttgggaaaa acgaatttat
    24421 atcatcgcgt gataatccat aggtagtttg agtaagatca ttggtaccaa aactaaaaaa
    24481 ctcagcatat tgagctattc gtcctgctcc aacagcagca gcaggcaatt caatcattgt
    24541 gccaacttca tattccaaat catcgatgcc gtactcttca actacctcat cttttatatc
    24601 ccgtatacct ttaactgttt tcccttcaat tcttttgcca tttttaataa acttaatttc
    24661 ggtttcgctc atgacaatgg gtatcataat ttcgggaaca acatcaattc cttctttctt
    24721 taacatgcat gccgcttcaa aaattgctct gcactgcatt tcataaattt caggatacgt
    24781 aattgctaca cgacagcccc tatgtcccag cattggattc atttcagata gctcttcaca
    24841 cctgattcta atttcagcat cagacatgcc ttttttacgc gacttcatat attgtatgaa
    24901 gtctttcatg ctatcatcag ttcttggcag aaactcatgt aaaggagcat ctaaaagccg
    24961 tatagtaact ggatgtccct gcataatttt aaataattca taaaaatcgc ttctctgcat
    25021 tggtttcaac tcatccaatg cctttcttcg ctcgacttca gtttcagata atatcatttc
    25081 acgaaatttc atgattcgtt tttcattgaa aaacatatgt tcagtacggc ataatcctat
    25141 cccatctgca aaaaacatct ttgcaacttt tgcatcacga ggctgatcag catttgcacg
    25201 tacatcaaag tctttaatga agctttggac tatctttaaa aagtcaatta aaccattctt
    25261 ttcaaaatca ggttcaatta agccaacttt atcaagataa atggtaggcg gttcataata
    25321 agggacatta atcgaaacat aatcaccttc tttaacggtt ttccctgcca gtgtaaacga
    25381 attccctcta atctttatct caggctgcac cattgcaacc ttacctaagc ttcgtgccac
    25441 aaccggagca tgtgaagaaa agccaccttc tgatgtaata accccacggg ctacctcgat
    25501 agctttcaca tcttctgcaa aagttgcagg cataacaagt ataaggttgg tatccccacc
    25561 atgcatcacc gaacgtttgt attcttccag cagcttgtcg gttgaaaaga atactcgtcc
    25621 tattgctgca ccggttgacc cggcaattcc accttttata gactttacat tctttgttgt
    25681 tcggggatca ataacagggt gtaaaagttc attaagttga ctgggcttaa tagattcaat
    25741 taaaaaattt gaacttacaa ccttacgtgt attaagatca aggagtgtct ttatatgtgc
    25801 ctgagttgat ttttcatcaa cctcgcgctg ttcaatgagc caaaaattac cctcttcaat
    25861 agtaaacttg atattccgta tctctttaaa gttatcctca actttttttg caatttcaac
    25921 taatttatca tagtattttt tatcaatctt tgcaatatct tttggagtgc cacggtcaac
    25981 atcaaactca ttctgcaaaa aatcgccctg aataagtgca tcaccggtaa taatattgcg
    26041 ggtaaaataa tttcctgcaa aggaattctg gccaaagttg ccatacacca ttgcctgaat
    26101 agttattgcc agtgaatcat caacatcaat atccgaatca cagtaccgtg cagcagcccc
    26161 tttcaaaata aggcctaact gatcatatac atcctcacta aacttatccc ccaaaaactt
    26221 tttatatttt gtaactactt ctttatattc ttttgctttt gcgtttttgg ggagcttccc
    26281 ttccagttca tccagttctt tttcatcaat accataaatc tttgaggcta tgcttctcag
    26341 taaaaaccta tactcacccc atgcaaaatc ttcaccggta aattttgcaa aagcagtaac
    26401 aattgcatca tttaaaccaa cattatgtat tgatggaaaa aatggaacat taagatctga
    26461 acttaacaca actttaagaa gaagtggttt ttcagtatca ccataccctt ttttcatgtc
    26521 cttttcaatt ttatcaacat agcttttaat aagctgttta acattcacct ttggcaattt
    26581 cattgttaac tctgcatcaa caataaatcc aggagctatt ggcaaaccca tctttgctaa
    26641 atcagcagca cgacgacctc taattcccaa ttctttatat tgctttttat caacaccggt
    26701 aaaatcttta ttaaaaaaaa ctatattttc catacaaccc tctcttttaa aatgttctat
    26761 cgcacattac attaaaatag atttaaaact tttcctactg ctatctttaa aaattgttca
    26821 aacctgggtg atgcaccttc aaacagatat ttctgtaatt ttgatacatt tctgatattt
    26881 tcaccaacca cctgaaataa gattggatca ccgtgtttaa cctttcccca tttaaagagg
    26941 gagttgatat cattaatctt ttcaccttca tggaatatca agacgtctaa atcaggatgc
    27001 gatactttaa agctttcaat aatacgcttc catgcttcca cgttcccgtt gtgaaataac
    27061 tcattggtaa ctgtcaccgc atatttggga gtcatatttt tctttcctgc tgcagccgct
    27121 tcagctggag caatttgctt tttggcctcc cggaccgcaa ctgtttcatc tatgaactct
    27181 ttattgataa aaacatcctt gccagccatc atctcttcaa ttgcatttat agcatcattt
    27241 tccacttttg gatttggttc tttctttttt ttaacgtaaa taaccataag ctggtcccta
    27301 acaatatccc tgacacgttc ccaatgaatc atatttttag gatttattaa aaaaggttcg
    27361 ctgttaacgg taaaatacac aagaattata tcaagtgctg tccattttga tgtttcttca
    27421 actattttgc tgaaatcatt aattgttacc ggtacattat atgaatcata ggaatactga
    27481 tatttatctt ttattattgc accgatcatt ggtaatacct ggtcaacagt taaatcccct
    27541 tcagccagca taacattgac tgcatctgca aaatttctac ctgattcaat ctcatccacc
    27601 acatcattaa ccctgtagaa atggtttaaa gccttattaa aattagtttt tgtagctaac
    27661 gaataaaatg ttgtttccat tgaattccct cttttttgaa ttattgataa taaaaaaagt
    27721 atacactaca tataatacat aatttaaaat aaattgttaa ttatttagaa ttttgataga
    27781 ttgtaattat aaagatagtt tttacaagta ttttttttat tttatgagca atcgcttcgg
    27841 acttattaac attgtataat cttataaggg taaataattg ctctaaagat aagtgggaaa
    27901 agaaaacagg tggcaattat catcgtatta aattcttgac aaaatggtat gatcgcacca
    27961 cagtggtgca tatggtttta aagcgggtgt agttcaatgg tagaacttca gccttccaag
    28021 ctgatagcgt gggttcg
//
LOCUS       DAOOJH010000025        28013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1779_length_28013_cov_9.446133, whole genome shotgun sequence.
ACCESSION   DAOOJH010000025 DAOOJH010000000
VERSION     DAOOJH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28013
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1779_length_28013_cov_9.446133"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLJ75_05215"
     CDS             complement(<1..1025)
                     /locus_tag="PLJ75_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPD04650.1"
                     /translation="MDSMLFWIAPIGSIIALLFAFVFYKLMMKADKGNETMVEIAQAV
                     RQGAFAYLRQQYKVVSLVFVILVILLSILAYYGIQNPFVPFAFLTGGFFSGLCGFLGM
                     NTATHASSRTAQGAKESLNRGLQVAFRSGAVMGLVVVGFGLLDISLWYIILNWVYDNN
                     IWGFSQSLAEKIGLLTAGQTFDISLLSHEAFKHGKLVEITTTMITFGLGASTQALFAR
                     VGGGIYTKAADVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYC
                     GSILSTAALGAALPLIGIENINAVIAPMIVAGLGILFSVIGIFFVRTKEDASMKTLLA
                     ALNRGVWG"
     gene            1282..2091
                     /locus_tag="PLJ75_05220"
     CDS             1282..2091
                     /locus_tag="PLJ75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPD04651.1"
                     /translation="MKNLAQLLLKLKQKKINKINNSKKSTNASPEKPIIQKQEMIQGI
                     LSLSQKNVREIMIPRVDINAIEDTATLKDLVKLIIDEGHSRIPVYSQTIDNIIGIVYV
                     KDLMPFIIDRRMKFNIKKLLHKPYFVPETMTLDELLLQFQKQNLHLAIVVDEYGGVAG
                     IVTLEDILEEIVGEIRDEYDEEEIPEIQTISRNCWEVDSRLSLSDFNNYTKCSLPEEE
                     FDTIGGFVFNLFGKIPKKDEQIKYNSITFKIKEIKGTRIGRIIVTISPEKM"
     gene            2104..2847
                     /gene="recO"
                     /locus_tag="PLJ75_05225"
     CDS             2104..2847
                     /gene="recO"
                     /locus_tag="PLJ75_05225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPD04652.1"
                     /translation="MELQKDAGIVLRKIPILEADIAATIYTNSHGKRTLLFKGLKKTK
                     RRSLTAVEPGSMVDVVYYYNEQKTMYNVKEYSLLYDTISIRNNYQAMITLFLLLEVVE
                     KTTGHNDPNQTLFKLLKGAIETLPKTSQYQILILSFILHYLKITGIYPDVFHCTVCHK
                     EITSDLYLSEFSLHSYCDACAPNNSFQFLSIVPVINDIMTNKFLTLESKHYPLPEINK
                     FIFHLLLFIDSYFTIHINSKELIFNNSIK"
     gene            complement(3035..3985)
                     /locus_tag="PLJ75_05230"
     CDS             complement(3035..3985)
                     /locus_tag="PLJ75_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPD04653.1"
                     /translation="MARILVVAEQRNGKISDATLELCKAAKALASEMGASPAAAVFYK
                     DDSLAKEVAQYIPEVFSVVDAKLEVYNADTYTAAAKAVVESQDVKGVLIPHTYDGTDY
                     AAKLAMAIGCGIVTNCNKCEAQGGTVVFTRNTYNGKIQEQRSVKTDKFVATFEKGAFE
                     KEAAGGAGSVTAVTASIPDARRKFKQFVETMSGSVDITQAKVIVSGGRGTKEKDKYND
                     VIVNFAKKLGGEYAASRPVVDAGWTDAARQVGQSGKTVTPDLYIACGISGAIQHVAGM
                     KGSKVIVAINKDPEAPIFNIATYGIVGDLFEVIPAIMENL"
     gene            complement(3997..4785)
                     /locus_tag="PLJ75_05235"
     CDS             complement(3997..4785)
                     /locus_tag="PLJ75_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPD04654.1"
                     /translation="MKIAVLVKQVPDMESKFKIIGDSKIDESQIAFKMNDFDEYAVEA
                     ALQLKEKFGGEVIIISAGPERASKEIRQAFAMGGDWGIQIQDEQVDAGDNFVVASALC
                     KAVESLGGVDLVLTGVQAEDDQAAVTGVMIADMLGMPHCTNVVKIEVGGDGKSMTVNR
                     ELEGGLNEVLEIAMPAVLTIQSGINQPRYPTLPGIMKAKKKRLDVKKAADLGVSAVGE
                     AGSKTKFVKMFFPVSDHKAEIIEGDPKSAAAKLVDKLRNEAKVI"
     gene            complement(4985..6979)
                     /locus_tag="PLJ75_05240"
     CDS             complement(4985..6979)
                     /locus_tag="PLJ75_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04655.1"
                     /translation="MSRKNIIIALILCCSFIVYGQEGIKLQQKNAIYHNKKGWESLQA
                     GNAYSAIVSFQSALSQNPKYKESLLGLGYAYVQTQGYNESIKLFDKVLKLYPDNEQAI
                     MGMAKAYTGLGRYKEAMKLYDRLNELSYANNESRFGMAYLYYKMGKMLWAKRLCDRIL
                     QNDPYYYDALLLYADIKAKDKRVDDAIDFVKKAIDAKDDYPDAFVKLAEMLYLKYVYS
                     GDSAYLLQATDELQKALVMNKTHLQANRDMGFLLYVQGNYQNAITCYESIVNNYPYIV
                     NNEVLYNAGLMYELTGNIMKALEYYTKALSKDNLNSMIAMKIRQLSLLEHIKIGHPVR
                     IQSSNDDFASSQELRKKGFPDLSMLFLRLSLALNPNNSTARQELLRIYETSGYFELYY
                     DEIKKMHSMNPTQKSQDLMSIAVFQRRDKLFFKLGYDKEEIPRDVPEVLVLDFTPDVP
                     VPAFADAGSVFANSINFAMGQFGRYQNFPMSQRLSIIKSLSSNNLDDILKNLNDKMNN
                     NEIPRISYLLYGEYNLSGRYIECNAKLMDAKTGVVIAHFTESDNSKYSTHTISINLAK
                     KMYDTIPFRGKIISYSEDMAVINLGTIDGIETGALLETYVENEDTIEQDQLRKKVVFK
                     VSETGTLISLVKPVNPKDIDLIAKGSDVFPVNKKRAKKIK"
     gene            7086..8096
                     /gene="mltG"
                     /locus_tag="PLJ75_05245"
     CDS             7086..8096
                     /gene="mltG"
                     /locus_tag="PLJ75_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPD04656.1"
                     /translation="MKEIKSLVVSLIIIVAIVIGIIWLLNHAPAGMHGDIITIRPGMN
                     LHQTAYYLKSKNVITSASFFIVMGRLLNETNVIAGNYRIYEGENSFSILHKITRGRFI
                     TKKVTIPEGFTMYDIARLLDSNDICSEGNFLYYAQSPAFMTSVGIPAPGAEGYLFPDT
                     YLLPENSDPRDVIIRLVNQTKKVITEIKNSVDGKNVSVHDMLVIASLVEREAKINSER
                     PLIAAVFYNRLRLKMRLDCDSTVQYGLKKFGKKLTYNDLNEDTPYNTYKFAGLPPTPI
                     CNPGKQSILAAYNPADVAYLFFVARNDGSHYFSKTLQEHSRAVDYYQKGIKNGFIDRQ
                     KH"
     gene            8068..9288
                     /locus_tag="PLJ75_05250"
     CDS             8068..9288
                     /locus_tag="PLJ75_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPD04657.1"
                     /translation="MALLTDKNIEVVAPAGNFEKLQFAVHYGAHAVYFGGQQFNLRAT
                     TKNFSVEDIEKAVSYAHNHQVKAIFLLNAFLHETDIQPVKEYIRLIKHMTFDAIMVSD
                     PGMALLLQEAGSTCKLHLSTQMSTLNHVAIQFWQKQGISRIVLARETTLDEIKMIRDH
                     TNCELEIFCHGALCIAYSGRCLLSRYLTGRDANQGNCAHPCRWNYSLVEEKRPGNHLE
                     VAQYNGTEILSSKDLCLIQRINEYIQAGVDAFKIEGRMKSLYYVANTVRIYRHAIDCY
                     NNGIDSSHYKNFWTNELDLVSHRPYTDDLFHEFTNQPFEGVPYIKKALFMGYCTQSDA
                     QKSTIKTFNPIYKGDNLDIIYPITNNGIRDSGAIVIDIEVDGKKCEMAQPNTYATVVF
                     DKPVDRWAIVRKRL"
     gene            9314..10567
                     /gene="hisS"
                     /locus_tag="PLJ75_05255"
     CDS             9314..10567
                     /gene="hisS"
                     /locus_tag="PLJ75_05255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPD04658.1"
                     /translation="MIQKPPGVEDIFPDRITRFNYIINTAKEVFRLYNYREVILPIME
                     YTEVFARGIGDATDIVTKEMFTFEDRGGRSLTLRPEGTASMVRAYVENGEYNRLSLCK
                     FFYCGPMFRAERPQKGRLRQFNQFGAELFGSDHPYFDFEILSIMNDIATKLHIHDYEL
                     LLNSIGCPDCRKPYITELFNYYKNHEHELCDDCKKRLYTNPLRLLDCKVESCVAIKQD
                     APKITQFLCKACNEHFDNVQKLLQKHTIKYSLHPLLVRGLDYYTKTTFEFVTAQLGGQ
                     NAFAAGGRYNNLVELFGGKPTPAIGFAAGIERIELLLEQTTLTDVLDVYIIYADETAS
                     SVVMHIANLLRKNNISCDFDPITKGFKTQFKRADREKAKFAIIVGQDELANNSVTIKD
                     MTSGEQIAILQSEVINFLLNKLTKQ"
     gene            10977..12812
                     /locus_tag="PLJ75_05260"
     CDS             10977..12812
                     /locus_tag="PLJ75_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04659.1"
                     /translation="MNTESKMKQFPFVQGLLAKEPHVYDILSEIVKMQELSLPDGKHE
                     MATLQFLQSSRNIPIPFTTSRNSQIGYLVTQLCNNYPDHVKKLITFDFHVEEKTKKLN
                     IIEKEEYCVDVLEEEFNRLQAMRDEISKQNLLALLKQYRLFNLSKLIKKSINKNDLNR
                     IIKSELGKTIFTFKHFTIDKSHVELAEDYCFHAENYQSVKSSINFKTLKNIISQHADM
                     LLRRLKRFGIISNEFSDYRDAKLDYIFTVLLEDLASLLNESDLAEVKNTHAFRSCLLK
                     VDALLDPVDTYNNDIVSFIHEHKLCTQVDIIDSLPDIDEELIEKWRESNYCKNAKVIA
                     FTNPEDNHRYYCDGTVLISYLSELNQLINYQRDKFEKLGYLEKDKIKKTYAILIKIAE
                     NIIAGDAYDELSLNSEQKQILQKIIDDFDKQKIEELPSQPVTIVKQTKQKKSLLKRFV
                     DAFLSLFKRKTIIKPITQTHVFSSPVHQLSKKTKNFYDTISSINKNIIAMTEVIDITD
                     ENESDINELIEELRNTGLKIVIPIYNARKVLYPRRSQKLLIPDIEYVLVSPVVMRSYD
                     AIKEFSDSLVGKKLNGEVIPASGIIAIEKYLYTLYRQKRNLMIKK"
     gene            complement(12826..13095)
                     /locus_tag="PLJ75_05265"
     CDS             complement(12826..13095)
                     /locus_tag="PLJ75_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPD04660.1"
                     /translation="MPTYEYVCTSCNNKFELFQSIKDEPVKTCAQCGGEVKRIIGAGA
                     GIIFKGSGFYVTDYKNNSNDKLIKSSSEPSCASCQSKTCEANTES"
     gene            complement(13304..15961)
                     /locus_tag="PLJ75_05270"
     CDS             complement(13304..15961)
                     /locus_tag="PLJ75_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD04661.1"
                     /translation="MGSIILFNSFSLGTIIPIIFFLTTGFFLYNTKDKSEATTILAKA
                     LIILGVFNFGYFISSSVYHPLAAYHRWLTILTILLGIAYLGYFYFFYPSPKAPRAAKI
                     YIIMGYILSFGALIAFIIVSLQSDIVFLFRGHYYDFDADEISKQIGVLIMVGILCSFI
                     VLWWRFFTSNKEEKKILLLLFLFTVIGTVVPSTTNTLSREGVIDRDVFHTAWTLFNLL
                     GFFLVFIVYINNSKERFSFLGKLIGIILVTVLCLLQIIGFIVLKKNEKDFDEIYYRSA
                     LLTVLNNQTMDNYIYHATYNIANNTWTQNLPDSYNKDDIAMEMKHAYTWNMIFSHAEN
                     NTVEGLQLLLDNTPVSFQGYVATLRYYIEQHKNTIDYSALMAFCRELDAKVYHFHHKI
                     SQLKGPNFRNDLKLLLPKQEPILQQFTSAIANYLETTSKEGEELKHDITQFLIPVQFP
                     GMRTYHVATDGQHVVAYRYINNEIIHEIAFPYTAYRSFMHSTVAILIAIVIIVYVFIR
                     FGIRLFFAGILVKPLRQLTDGLKEVDKGNFDVAIPVQSGDEFGYITSAFNKMVESLKN
                     LFQSTHAKSEEVKRLSSDLNISATKLYDIARELSTIVEETSSAYEEMSSSFESNLNAI
                     QQQANSMDVIKNDIESIDASSSQVSQRITRLSNSIEQAVKQSEEGEVTITKSINAIES
                     IAEYLKEVEQTIVNINEIADKINLLALNAAIEAARAGESGKGFSVVADEVNKLADQTT
                     DIVKGIHATIADQAKMITNEIQYISKATEAIKAIRKGIGETFNVLKDTVDFTNELGIK
                     NQDIRSKLEKFKELSGSVYNFSMEQKVTIDELTKAVNAIIEISQKALESAEFVQGFSK
                     ILDLSAQELSESIGYTANSNSNSESDNNT"
     gene            16220..16292
                     /locus_tag="PLJ75_05275"
     tRNA            16220..16292
                     /locus_tag="PLJ75_05275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16253..16255,aa:Thr,seq:cgt)
     gene            complement(16302..17612)
                     /locus_tag="PLJ75_05280"
     CDS             complement(16302..17612)
                     /locus_tag="PLJ75_05280"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPD04662.1"
                     /translation="MINQRQKTVVIIGAGFGGLWVAKELSNTNYNVILIDKNNYHTFL
                     PLLYQVSAAEIEPEQIASPVRFIIRNTKNIRFIRGEVTAIHYANKTIECLGQHIPFDY
                     LVVAAGSVNSYFNISGASQYSFPLKTLDDAMILRNHVLSCFERASFIDDKNQRKRLLT
                     IVIVGGGPTGVEFAGALAELVHGPLHKDFPELDYEEISVCLIEAGDRILSMYPPILSE
                     YAKSELIKKGVNVLTHANVSKIEATGVYLNDSPIIPTETVVWTAGVKGEMIKSDCNLK
                     ISTNGRIQVNDNLNIPGYDDVFIIGDLAQYSQDGKTLPMIAPVAIQQGKYVGRLLQLI
                     AKNKKAKPFHYKDKGSMVTIGRNHAVTQVAGLHLKGYIAWIIWIFIHILYLIGFRNKL
                     FVMINWVWDYIFFEKSVRLILPRCCDNPDNTTCKRRFPNNHFCN"
     gene            18394..19257
                     /locus_tag="PLJ75_05285"
     CDS             18394..19257
                     /locus_tag="PLJ75_05285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD04663.1"
                     /translation="MNNSLYDSNNSLLKPADVFANNDEYIAYIQTLTIPGVKFTIQTI
                     EKGDNFWKIAKEHKIDIDTLIGANPLWESITAKVDQKIVIPSQKGVLHFVADINEINS
                     LHSLYDVSEKNVLTPSLFLLRLQSIVSKPEKPIAVFVVDARPKADVMTPQLSKQFTIR
                     EMFRSPLGGRLTSFYGGRLHPIFRQWGFHNGLDIAAPYGTPVGAARGGVVISCGWMGG
                     YGKAVIIQHDEGFKTLYGHLSSIHVRPGQHIKAGQFVGRVGSTGWSTGPHLHFTLWQY
                     NKLINPMKVLW"
     gene            19268..20713
                     /gene="rsmG"
                     /locus_tag="PLJ75_05290"
     CDS             19268..20713
                     /gene="rsmG"
                     /locus_tag="PLJ75_05290"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPD04664.1"
                     /translation="MYQKLIKEYFEKASLYYTKEIISRFNQFLHLLLQYNTSHDLTRI
                     TSIDEIIIKHFVDSLFITKFITIPSPLLDIGTGAGFPGIPLAIVYPDKRFILAESRHK
                     RTQFLSIVQNSLQLNNIEIYPHLVTEKSFFEVSGVITRAVESIDETLHRCHHFLQQGN
                     IVLLMKGPSVDEENITLCDSYSKIMDQPYTLPATSYKRRLIVYKKTDGIIKKTYFINK
                     EGRSGDGLAITSGDNKRYRELKKIITDPRKCSMSTVVGKKIIKELVSSHPQLCRYIVL
                     YDEYAETDTELMQIIADFGHQRIILLKKALFNEIDVLGNNQPILVIDVPPITPWNMDT
                     QGLSLMIPFQDPVNVGAAIRSAVAFGVNNIIICKNAAYPFHPKAIRSSAGAVFHCTLW
                     QGPPLYQHQPDFIELSYPIIALDLEGINIYDFTFPEDAVLLAGVEGQGLHSIKNCIRV
                     TIPIQVVESLNATVALSIALYEWHKQTTISC"
     gene            complement(20700..22136)
                     /locus_tag="PLJ75_05295"
     CDS             complement(20700..22136)
                     /locus_tag="PLJ75_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPD04665.1"
                     /translation="MAITQLPPNGIEYNKVLSMMKQYRKDDANWKQGKTFSLVYHRDD
                     AHTKFLQKAFELYFDENALNPMAFKSLKKFENEVVKMTANMLHAGPEACGTMTSGGTE
                     SCLLAVKTYRDRARSLHPWIKKPEMIVPESIHVAFEKGAEYFDVKIIRAPLDKNFKVD
                     VNAVKKLINRNTILIIGSAPCYPYGTVDPIAELGSIAHLKKIPLHVDACLGGFLLPFV
                     EKSGYDIPPFDFRVNGVTSMSADVHKYGFSAKGASVIVYRSIDYLRHQFFIYENWPGG
                     VFASPALLGTRPGGTIAAAWAAMVAQGEKGYIKNAKIIMDTSRKIQQGIESIKGMEIV
                     GKPESSVFAYRSIEPDVNTFAIGDILEKKGWHVDRLQRPDALHAMITPIHAKVAQQFI
                     NDVKEAFTTVRKNPELAYEGGAAMYGMISHIPMRKMIRKNIAKMMEGMYGPDGAIPDL
                     SMDETGGEDFAMKAGKWYLKLKDTFSKK"
     gene            complement(22181..23344)
                     /locus_tag="PLJ75_05300"
     CDS             complement(22181..23344)
                     /locus_tag="PLJ75_05300"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HPD04666.1"
                     /translation="MNTAIFLLLICFYLLIPALIIYVCYKFPSINKIGAVLLCYSAGI
                     TIGNIGILPEQAKSIQDISSNIAIILGLPMLLFTINIKTWFRLAGKALLAFTLECIAV
                     VIVVFAGYLLIRHIIPDAWKIAGMLLGVYTGGTPNLAAIKEAFNVDSTTFVIVHTYDT
                     VLSMVYIIFLVTIAQKLFLKFLPPFKVINNENEEDKNAKTGEDIRLYGDIFSKNILKN
                     LVIALLLSIVVVAISLGIAGLLPNQYQTMVVILAVTTIAILLSLIPYVHNLKRTFQFG
                     MYLIYIFCTIVGSMVNVDNIIHINIPLLFYVFLAIFGSLLVHGILCKIFSIDTDTYII
                     TSVAGICSPPFVPVVADALHNKYILLSGITTGVIGYAIGNYLGITLGYVFSSI"
     gene            23485..24138
                     /locus_tag="PLJ75_05305"
     CDS             23485..24138
                     /locus_tag="PLJ75_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04667.1"
                     /translation="MKKEERIKWDKEQRTARIIEIAQELFFTRGFDATTIDEIAHLAG
                     YNKRTLYHYFKDKEELFLAVALNGLKLFNEKLEEAFNYPLEGHTRLYSMGKAFYNFFL
                     KYPDYFNLIMTYEARNCMYYPVTNSNTMSFYKLECQKIADSDTELIIKAIEYSINDGI
                     IKTTLTPKQLMLILWGQVFGITQIIMIRKDFFKEAYGIDHEELFETFLRETEKALKN"
     gene            complement(24210..24410)
                     /locus_tag="PLJ75_05310"
     CDS             complement(24210..24410)
                     /locus_tag="PLJ75_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006243848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPD04668.1"
                     /translation="MPKGTIKWFNEKKGFGFISKEDGSDVFVHYTGIIGNGFRTLKEG
                     QRVEFEIESSDKGPRAVSVKAI"
     gene            25000..25749
                     /locus_tag="PLJ75_05315"
     CDS             25000..25749
                     /locus_tag="PLJ75_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HPD04669.1"
                     /translation="MRIIAISNNKGGVGKTTSTVNIAAALQIIGKKVLVIDIDHQAQS
                     TYHFGYNPHNLTYSIYNILKGEIAYKEILIDRNGLHILPSKNDLKDIEFIPLPAKEFL
                     LKEALENISGYDFILIDCPPSLGILTLNALAFAKEIFVPLQVQFLPFHGMYNLFEAVQ
                     MVKRRLNKEIEITGIIGTMYNPKKQINVEVIDETKKRLPGKLFETLIRENVLLQEAPS
                     WGKTIFEYKPNSNGADDYMNLTKEILERSPD"
     gene            complement(25753..26823)
                     /locus_tag="PLJ75_05320"
     CDS             complement(25753..26823)
                     /locus_tag="PLJ75_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HPD04670.1"
                     /translation="MENYIDKPADIREGEDIDKHILFDYLVNNVSDIKGTIEIKQFPG
                     GFSNLTYFIKIGDRELVLRRPPFGKKAKTAHDMHREFVILKALKPVFPYCPAPLVYCD
                     DESVIGAPFYVMERIKGIILRKELPQGLSYTPDEFKYLCEQLLDIQCLLHSLHYKELG
                     LENFGKPDGYIERQVHGWINRYNDALTPDAPSYTEIITWLKEKLPETPTRAAIIHNDY
                     KFDNVVLDNKNPKKIIGVLDWEMATIGDPVMDLGNSLAYWVEKEDPEDMQLIRLMPTH
                     LDGALTRKELVNRYHEKSGIDMSDFDYYYCFGLFRLAVIAQQIYYRFYHGQTKDKRFS
                     TLILAVQILEKQAKRVIEKSHL"
     gene            complement(27248..>28013)
                     /locus_tag="PLJ75_05325"
     CDS             complement(27248..>28013)
                     /locus_tag="PLJ75_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04671.1"
                     /translation="IKLQWQKGYCQAMKAEKSDNFNELFIDNYNNFRKSIKIVLDEIV
                     TKLYDDNKQLIKVKKSDQSINNLKRIINTAISISNKKGYAAMSMRELSRASSLSIGAL
                     YSYFPSKDELLHIIQQYGRAAVEKTLSSALSKASSTKDKLYNFIHAHIFISEALKDWF
                     YFSYMETRYFTGIEYNKTIESELFTESLIDSIIKDGIKKSECKSNLNSSLIASVTKAM
                     MQDWYLKRWKYKRRNILPEEYALECFNIIYNYIHNG"
BASE COUNT         9326 a         5113 c         4464 g         9110 t
ORIGIN      
        1 ccccatacac cacggtttaa tgcagcaagg agagttttca ttgatgcatc ttctttagtt
       61 ctcacaaaaa agatacctat aactgagaac agaataccca atcctgcaac aatcatagga
      121 gcaattactg cattgatatt ttcaatgcca ataagtggca gtgcagctcc taatgcagcg
      181 gtagaaagta tagaaccaca atatgattca taaaggtcag cacccatacc tgcaacatca
      241 cctacattat cgccgacatt atcagctatg gtagcaggat tacgtgggtc gtcttcaggt
      301 ataccagctt caactttacc aacaaggtct gcaccaacat cagcagcttt tgtatagata
      361 ccgccaccaa cacgagcaaa tagtgcctgt gttgaagcac ccagaccaaa agtaatcatg
      421 gttgttgtaa tctcaacaag cttgccatgc ttgaaggctt catgacttaa caatgaaata
      481 tcaaaagttt gaccagcagt caaaagacca attttttctg ctaatgactg gctaaatccc
      541 cagatattgt tgtcgtaaac ccagtttaaa ataatatacc ataaagatat atcaagcaaa
      601 ccaaagccta ctactactaa tcccataaca gcacccgatc taaatgctac ctgtagtcct
      661 ctattaagcg attctttcgc accctgtgcg gttctagatg aagcatgagt cgctgtattc
      721 atacctaaga aaccgcataa acctgagaaa aaaccacccg ttaggaatgc aaatggtacg
      781 aatggattct ggattccata ataagcaagg attgataaaa gaattaccag gatcacaaat
      841 actaaagaaa caaccttgta ctgctgtctt aaataagcaa atgccccttg acgcacagcc
      901 tgtgctattt cgaccatcgt ttcatttcct ttgtctgctt tcatcatcaa tttataaaag
      961 acaaaagcaa acaaaagagc aatgatcgac ccaattgggg ctatccagaa aagcatacta
     1021 tccatatggg ccactccttt tttattattt tttaaaatgt aaaataaact ataatatatt
     1081 cttttgatgt ctaaaaaaaa gaaataccat ctattgtcaa gtgtatttta taattaaaat
     1141 tttttcctgc tattttacat tctttaaaat ataaaatcaa attgacatat ttcatataaa
     1201 tataccaata tgttaacaat ctttttgagg aggttattta agatagtatt tatgtcttca
     1261 ggccagcaaa agtccccgat aatgaaaaat ttggcacaac ttttgttaaa actaaaacaa
     1321 aagaaaataa ataaaataaa taatagtaaa aaaagcacca atgcatctcc tgaaaagcct
     1381 atcatccaaa aacaggagat gatacagggt attttaagtc tttcacaaaa aaatgtccga
     1441 gagatcatga ttccccgtgt tgatataaat gccattgaag atactgctac ccttaaggat
     1501 ctggttaaac ttattataga tgaaggacat tcacgtatcc ctgtttattc acaaactata
     1561 gataatatta ttggtattgt ctatgttaaa gatttaatgc cgtttattat tgaccggcga
     1621 atgaaattca acataaagaa gttgctgcat aaaccctatt ttgtccccga aacaatgaca
     1681 cttgatgagt tgctccttca atttcaaaag caaaatttgc atctggcgat agttgttgat
     1741 gaatatggtg gtgtagcagg tattgttact ctggaagata ttttagaaga gatagttggc
     1801 gaaatacgtg acgaatatga tgaagaggaa atccctgaaa tacaaacgat atcacgcaat
     1861 tgctgggaag tggattcccg tttatcacta tctgatttta ataattacac caaatgctca
     1921 ttgcccgaag aagagtttga tacaattgga ggttttgtct ttaatctttt tgggaagatt
     1981 cccaaaaagg atgaacagat aaaatacaat tctataacat ttaaaataaa agaaattaaa
     2041 ggcactcgca taggaagaat tatcgttacc atatcacctg aaaaaatgta aataacgtat
     2101 aaaatggagc tacaaaaaga tgctggtata gtattacgta aaattccgat attagaggct
     2161 gatatcgctg caacaatcta tacaaatagt catggcaaaa gaacattact ttttaaaggt
     2221 ttaaaaaaaa ctaaacgacg gagccttacc gctgttgaac caggaagtat ggttgatgtt
     2281 gtctattact ataatgagca aaagacaatg tataacgtta aagaatattc tttactctat
     2341 gataccatat ccattcgcaa taactatcaa gcaatgataa cccttttttt attacttgag
     2401 gttgtagaaa aaaccaccgg tcacaatgat cccaatcaaa cgctatttaa attattgaaa
     2461 ggcgccatag aaacattgcc aaaaacaagt caatatcaaa ttctaatttt atcatttatt
     2521 ctacattacc ttaaaatcac aggaatttat cctgatgttt tccattgtac tgtttgtcat
     2581 aaggaaatca ccagcgattt atatcttagt gaattttcat tacattccta ttgtgatgca
     2641 tgcgcgccaa ataattcatt ccaatttttg tcgatagttc ctgtaatcaa tgacatcatg
     2701 acgaataagt ttcttactct ggaatcaaaa cattacccat tacctgagat aaataaattt
     2761 atttttcatt tattactttt tattgattca tattttacta tacatataaa ttcaaaggaa
     2821 ttgattttta ataacagtat taaataagag tatcgatgaa aaatattttt attttgcctg
     2881 tagacagtat atatttgtac aatcatattt cattgctatg aggaacttct catcattagg
     2941 ttaatttttt taaataaatt gccatatata aatataaagg gcacggtaat tatccgtgcc
     3001 ctttatatat cgtttataaa tagttgaaat aggtttacag attttccatt atagcaggta
     3061 ttacttcaaa aaggtcacct acaataccat aggtagcaat attaaaaata ggagcttctg
     3121 gatctttgtt aatagcaaca ataaccttag aacccttcat tccggcaaca tgctgtatag
     3181 caccggaaat accgcatgca atataaagat caggagtaac agtttttcct gactgcccca
     3241 cctgccttgc tgcatctgtc cagcctgcat caacaactgg acgtgacgct gcatattcac
     3301 cacctaattt tttggcaaag ttaacgataa cgtcattata tttatctttt tcctttgttc
     3361 cacgaccacc tgatacaata acctttgcct gagtaatatc aaccgaacct gacatcgttt
     3421 caacaaattg tttaaatttt cgacgcgcat cgggaataga tgcagttaca gcagtaaccg
     3481 aacctgcacc accagcagct tctttttcaa aagcaccttt ttcaaacgtt gcaacaaatt
     3541 tatcagtttt tactgaacgc tgctcttgaa tcttcccgtt gtaggtattt cgcgtaaaaa
     3601 caacagttcc gccctgagct tcacatttat tacagttagt tacaatacca catcctatcg
     3661 ccatggctaa ttttgcagca tagtcagttc catcataggt atgtggaatt aaaacgcctt
     3721 taacatcctg actttcaaca acagctttcg ctgcagcagt atacgtatct gcattataaa
     3781 cttccagctt tgcatctact actgaaaata cttcaggtat atactgagca acttcttttg
     3841 ctaagctatc atctttataa aaaactgcag cagcagggga tgcacccatt tcagatgcta
     3901 aagccttagc agccttacaa agttctaatg tagcatcgct gattttaccg tttctttgtt
     3961 ctgcaacaac taatatacgt gccattgtgt acccccttat atcacttttg cttcatttcg
     4021 taatttgtca acaagcttgg cagcagctga ttttggatca ccttcaatta tttcagcctt
     4081 atgatccgat accgggaaga acatttttac aaactttgtt ttagaccctg cttcaccaac
     4141 agcagatacg cctaaatcag cagccttttt gacatccaat cttttctttt tggccttcat
     4201 aatcccggga agggttgggt atcttggctg atttatacca gactgtatgg taagaacagc
     4261 cggcatagct atttctaata cttcattcaa tccaccttca agctctctgt tgacagtcat
     4321 tgatttgcca tcaccgccaa cttcaatttt aacaacattg gtacaatgtg gcatacccag
     4381 catatcggca atcattactc cagtaactgc cgcctggtca tcttcggcct gaacaccggt
     4441 taatacaagg tcaactccac ccaggctttc tacagccttg caaagtgctg atgcaacaac
     4501 aaaattatca cctgcatcaa cctgttcatc ctgaatctgg atgccccagt cgccacccat
     4561 tgcaaaagcc tgacgaattt cctttgatgc tcgctccgga cctgctgaaa taataataac
     4621 ctcaccacca aacttttctt ttaattgcag cgcagcctca acagcatact catcaaaatc
     4681 attcatttta aaagcaatct gggattcgtc gatcttactg tcgccaataa ttttaaattt
     4741 tgattccata tctggaacct gcttaactaa gacagctatc ttcatagtcc cgctcccttt
     4801 tttaaaattt tatacaaaaa aatagaataa gcacatttat gcacaatggt ataaattgct
     4861 ttttatatgt caagatttat tattaaatta caatatagaa atttagttac agaaatttta
     4921 tatacactaa ataatatggt tctatttact ataaatggta ggtaatttta agttattaaa
     4981 attgctactt tatttttttt gctcttttct tattcactgg aaatacatct gaacctttag
     5041 caattaaatc aatatctttt ggatttacag gttttactaa agagatgaga gttcctgttt
     5101 ctgaaacctt aaaaacaact ttttttctta attgatcctg ttctatggta tcttcatttt
     5161 ctacatacgt ctctaaaagg gctcctgttt ctatgccatc aatagttcct aagtttatga
     5221 ctgccatatc ttccgaatat gatataattt ttcccctgaa tggtattgta tcatacattt
     5281 tttttgccag attaatcgaa atggtatggg ttgaatattt actattatcg gattcagtaa
     5341 aatgagctat caccacaccg gtctttgcgt ccatcagttt agcattacat tcaatatatc
     5401 ttcctgaaag attatactca ccgtatagta aatacgaaat ccggggaatt tcattgttgt
     5461 tcattttatc attaagattt ttgagaatgt catccaaatt gtttgacgat aagcttttaa
     5521 tgatagataa cctttgagac atggggaaat tttgataacg tccaaattga cccattgcaa
     5581 agttaataga atttgcaaag acgctgccag catctgcaaa agcaggaaca ggaacatcag
     5641 gagtaaaatc aagcaccaat acctcaggaa catcacgggg aatctcctct ttatcatatc
     5701 ccagcttaaa aaaaagttta tccctgcgct ggaatactgc gatgctcatt aagtcctgcg
     5761 atttttgagt tggattcatt gaatgcattt tctttatttc atcatagtat aattcaaaat
     5821 aacctgaggt ttcatatata cgcaatagtt cctgacgtgc tgtactattg ttgggattta
     5881 atgctaatga taatcgcaaa aatagcatgg ataaatcggg aaagcctttt ttacgtaatt
     5941 cctgcgatga ggcaaagtca tcattgctac tttgaatccg aacaggatgg ccaatcttaa
     6001 tatgttcaag caagcttaat tgccgtatct tcatggctat catactatta agattatctt
     6061 ttgaaagagc ttttgtataa tattccagag ctttcataat gtttccggta agctcgtaca
     6121 taagccctgc attatacaat acttcattat tgactatata gggatagtta ttaacaattg
     6181 actcatagca ggtaatagca ttctggtaat tgccctgaac atacaacaaa aaacccatat
     6241 cgcggtttgc ctgtagatgt gttttgttca taacaagggc tttttgtaat tcatcggtag
     6301 cttgtaataa ataggcacta tcgccagaat aaacatattt taggtataac atctcagcta
     6361 acttaacaaa agcatcagga taatcatcct ttgcatctat tgccttttta acaaaatcta
     6421 ttgcatcatc aacacgttta tccttagcct taatatcagc atataatagc aatgcatcgt
     6481 aatagtaggg atcattttgt aaaattctgt cacacaaccg ttttgcccac agcattttcc
     6541 ccattttata gtacagatac gccataccaa aacgcgactc attgtttgca tacgacagct
     6601 catttaatct atcatataat ttcatcgctt ctttatatct gccaagtccg gtatatgctt
     6661 ttgccatgcc cataatggcc tgctcattat ccggatagag cttcaatact ttatcaaaca
     6721 actttattga ttcattgtat ccctgagttt gcacataggc ataccccaaa cctaataatg
     6781 attctttata tttggggttt tgcgaaagtg cactttgaaa cgatacaatt gctgaatagg
     6841 cattgcccgc ctgcaagctt tcccatcctt tcttgttgtg atatatggca tttttttgtt
     6901 gaagtttgat accttcctgt ccatacacta taaacgaaca acaaagtata agtgctatta
     6961 tgatattttt tctgctcatg tgttttttta ttgataaaat aatagtattg aaaatcaaag
     7021 taattaaata gctaccttac aatttaagtc aacacattat ttatttaaag ttaaagagcg
     7081 tttaaatgaa agaaattaaa tcactggttg tatcactcat aattatagtg gcgatagtta
     7141 ttggtattat ctggttgctt aaccatgctc cagcaggcat gcatggagat atcatcacca
     7201 tacgtcccgg gatgaatcta catcagactg catattatct taaatcaaaa aatgtcataa
     7261 caagcgcttc attttttatt gttatgggaa gattactcaa cgaaacaaat gttattgctg
     7321 gtaactatcg catatatgaa ggtgagaata gtttttcaat attacataaa ataacaaggg
     7381 gaagatttat tacaaagaag gtaaccattc ccgaaggctt taccatgtat gacattgcca
     7441 ggttgcttga tagcaatgat atatgttcag aaggtaactt tttatattat gcacaatcac
     7501 cagcttttat gacatcagtt ggaataccgg ctcccggtgc tgaagggtat cttttcccgg
     7561 acacctacct gctaccggag aatagcgatc ctcgcgatgt tattatccgt ctggtgaatc
     7621 aaacaaaaaa ggtaatcacc gaaataaaaa attctgttga tgggaaaaat gtatcggtgc
     7681 atgatatgct tgtcattgca tcgcttgttg aacgagaagc taaaatcaac agcgaaagac
     7741 cattgattgc tgccgtcttt tataatcgct tgcgattaaa gatgcgattg gattgtgact
     7801 ctaccgtaca atatggctta aaaaaatttg gtaagaagct tacctacaat gacctgaacg
     7861 aggatacacc gtacaatacc tataaatttg cagggcttcc tccaacacca atctgcaatc
     7921 ccggtaaaca atcgattctt gctgcataca acccggcaga tgttgcctac ttattttttg
     7981 ttgcacggaa cgatggatca cattattttt caaaaacatt acaagagcat tcaagggcag
     8041 ttgattatta ccagaaagga ataaaaaatg gctttattga cagacaaaaa cattgaagtg
     8101 gtggcacctg ctggtaattt tgaaaaactg caatttgctg ttcactatgg tgcacatgcc
     8161 gtctactttg gcggtcagca atttaatttg cgtgcaacaa ccaaaaattt ttctgttgaa
     8221 gatatagaaa aggcagtatc ctacgcacac aaccatcaag taaaagcaat atttttactc
     8281 aatgcttttc tccatgaaac agacatacaa cccgttaaag aatacatacg acttataaaa
     8341 catatgacat ttgatgcaat catggtatct gaccccggaa tggctttgct tttacaggag
     8401 gctggtagta cctgtaagct gcacctttca acgcagatgt ccactctcaa tcatgtagct
     8461 attcaattct ggcaaaaaca ggggatatct cgtattgttt tagccaggga aacaacactg
     8521 gatgaaataa aaatgatccg tgatcacacc aactgtgaac tggagatctt ttgccatggt
     8581 gcgctgtgca ttgcctattc gggacgatgc cttctcagcc gctacttaac aggacgcgac
     8641 gcaaaccagg gtaattgcgc acatccctgc cgctggaact attcactggt agaggaaaag
     8701 cgtccaggca atcatctgga agttgcacag tataatggaa ctgagatact atcatcaaag
     8761 gatctatgcc tcattcaaag gatcaatgaa tatatacagg caggtgttga tgcttttaaa
     8821 attgaaggca ggatgaaatc actctattat gtagccaata ccgtccgcat ctatcgccat
     8881 gctatcgatt gctataacaa cggtattgac agcagccatt ataaaaattt ctggacaaat
     8941 gaattggacc tggtaagcca cagaccgtat actgatgacc tcttccatga gtttaccaat
     9001 caaccatttg aaggagtgcc ttatatcaaa aaagcattgt tcatgggata ttgcactcaa
     9061 tccgacgcac aaaaaagtac tattaaaact ttcaatccta tttataaagg tgataatctg
     9121 gatatcatct atcccataac aaacaatggt ataagggata gcggggcgat agttattgac
     9181 attgaggttg atggcaaaaa atgtgaaatg gcacaaccaa acacctatgc taccgtagtg
     9241 tttgataaac ctgttgacag atgggcaatt gtgcgcaaac gattatagat tatactggat
     9301 aaggagaacc gcaatgattc aaaaaccgcc aggcgtagag gatatattcc ctgaccgaat
     9361 taccagattt aattatatta tcaataccgc aaaagaggtt ttccgactct ataattaccg
     9421 agaggtgata ttacccatta tggagtatac agaggtgttt gcaagaggga ttggcgacgc
     9481 aacggatatc gtgacaaagg agatgtttac ctttgaagat agaggtggta gaagcttaac
     9541 actcaggccc gaaggtacag cttccatggt tcgtgcctat gttgaaaatg gcgaatataa
     9601 tcgtttatca ctgtgcaaat ttttttattg tggacctatg ttcagagcag aaagacctca
     9661 aaaaggaaga ttgcgacagt ttaaccagtt tggtgcggag ttatttggta gtgatcatcc
     9721 ttattttgat tttgaaatac tatccattat gaacgatatt gctaccaaac tacacattca
     9781 tgattatgag cttttattaa attcaattgg ttgtcctgac tgccgaaaac catatattac
     9841 cgagttattc aattattata aaaaccatga gcatgaactt tgtgatgatt gtaaaaaacg
     9901 tttatatacc aatccattac gattattaga ttgtaaggtt gaaagctgtg ttgccataaa
     9961 acaggatgcc ccaaagataa cacaattttt atgcaaagct tgtaacgagc actttgataa
    10021 cgttcaaaag cttttacaaa aacatactat caagtatagc cttcatccac ttctggtacg
    10081 tggtttagat tattatacaa aaacaacctt tgaatttgtt accgcgcaat taggtggaca
    10141 gaatgcattt gctgctggtg gacgatataa taatctggtt gaattatttg gaggcaaacc
    10201 cactcctgcc ataggttttg ctgccggtat tgaacgtatt gaattgttgc tggagcaaac
    10261 gacgctcacc gatgtattgg atgtatatat tatttatgcc gatgaaactg catcatcggt
    10321 agttatgcac attgccaatc ttctaaggaa aaacaatata tcctgtgatt ttgacccaat
    10381 taccaaagga tttaaaacgc agtttaaacg tgctgatagg gaaaaggcaa aatttgccat
    10441 tatagttggt caggatgaat tagccaataa ctctgttacc attaaggata tgacatctgg
    10501 tgaacagata gcaatcttgc aatcagaggt tataaacttt ctattaaata aattaacaaa
    10561 acaataatta ttattgaaac tccacttttt acaatctctt ttctacataa tattaaaact
    10621 gtcacaatac agtggttttg aaatattgta cttcagcata aagtctaaat tttaatcttt
    10681 caaatatcca cttgttctat ttatttctat ttagctgtgt ttattgtgta ttattataca
    10741 tggtttatgt tggtttatgt tggtttatgt ttgatatatt cattataaac atttgttgct
    10801 aaataaaaat cctttttttt aataaagtac tttacttaat ttatagtacc taccatattt
    10861 tgaacaacta tctttcaaga ttttgtaaat ggcaatcatg agcaatcaat acgttttgct
    10921 gacacactat gatacctatc tatgtttttt tatcttaaaa tgttcaccat tttaatatga
    10981 acacagaaag taaaatgaaa caattcccgt ttgttcaagg gctactggca aaggaacctc
    11041 atgtttatga tattttatct gaaatagtaa aaatgcagga attatcactt cctgatggca
    11101 aacatgaaat ggctacactg cagtttttac aatcctcgcg aaacattccc atacctttta
    11161 ctacttctcg caacagccag attggttatc ttgtaacaca gctttgcaac aactatcccg
    11221 atcatgtaaa aaagctgata acctttgatt tccatgttga agaaaaaaca aaaaaattaa
    11281 acataattga gaaagaagag tactgcgttg atgttcttga agaagaattt aaccgtttgc
    11341 aggcaatgcg agatgaaata tcaaaacaaa atttattagc gctgttaaag caatatcgtc
    11401 tttttaatct ttcaaaactt ataaagaaat ccattaataa aaatgactta aacaggatta
    11461 ttaaatcaga attgggtaaa acaattttta cattcaagca ttttaccata gataaaagtc
    11521 atgttgagct ggctgaagat tactgcttcc atgctgaaaa ttatcagagc gttaaaagtt
    11581 ctattaattt taaaacattg aaaaatataa tatctcaaca tgctgatatg cttttgcgca
    11641 ggcttaaacg atttggtatt atatcaaatg agttcagtga ttaccgtgat gccaagctgg
    11701 attacatatt tacggtgctt ctggaagatt tagcatcgct acttaatgaa agtgatttag
    11761 cggaggttaa aaatactcat gctttcagat cttgtctatt aaaagttgac gccctgcttg
    11821 atcctgttga tacctataac aatgatattg tttcatttat acatgaacac aaattatgta
    11881 cgcaggtaga tattattgat agtttgccag atatagatga agaactcatt gaaaaatggc
    11941 gtgaatcaaa ttattgtaaa aatgcaaagg taattgcttt tactaatcct gaagataatc
    12001 atcgctatta ttgtgatgga acggtattga ttagttatct gtcagagctt aatcaattaa
    12061 tcaattatca gagggataaa tttgaaaagt taggttatct ggaaaaagat aaaataaaaa
    12121 aaacatatgc tattttaatt aaaattgctg aaaatattat tgcaggggat gcatatgacg
    12181 aactatcact taatagcgaa caaaaacaaa ttctacaaaa aattatagat gactttgata
    12241 aacaaaagat tgaagagttg ccatcgcagc cggttaccat tgtaaaacaa acaaagcaaa
    12301 aaaaatcatt attaaaacga tttgttgatg catttctttc tctatttaaa agaaagacaa
    12361 taataaaacc tattacacaa actcatgtat tttcatcacc ggttcatcaa ctaagtaaaa
    12421 aaacaaaaaa tttttatgat actatcagct caattaataa aaatattata gccatgacag
    12481 aggtcattga tattactgat gaaaatgagt cagatataaa tgaattaatt gaagaattaa
    12541 gaaatacagg attaaaaata gtaatcccca tttataatgc acggaaggtg ttatatccac
    12601 gaagaagcca gaaattatta atcccggata ttgaatatgt tcttgtatct cccgttgtta
    12661 tgcgatcgta tgatgcaata aaagaattta gcgattcact tgtaggaaaa aaactcaatg
    12721 gtgaagttat tcctgcttct ggtattattg cgatagagaa gtatctgtat acactctatc
    12781 gccagaaaag aaatctgatg ataaaaaaat agttattcta ttattctacg attctgtatt
    12841 agcttcacat gttttggatt ggcatgaagc acatgaaggc tctgaagaag atttaattaa
    12901 cttatcatta gaattattct tatagtcggt tacataaaag ccggatcctt taaaaataat
    12961 tccggctcca gcacctataa tacgtttaac ttcacctccg cattgagcac atgtttttac
    13021 tggttcatcc tttatagact gaaacaattc aaatttatta ttacaagagg tacaaacata
    13081 ttcgtaggta ggcatttcat ccctccatat tacgcataaa tagtataatt ttcatgaata
    13141 taattaatat atttaattga atgattaata ctataaataa tgtatacaaa tatagcaaca
    13201 aatcaataat ttttctttaa aaaattttaa agtaacaaat ttataaacat aaaagatgtt
    13261 gtactttata ctacaatcta aatactacaa tccgatatac aaattacgta ttattgtcag
    13321 attctgaatt agaattagaa ttagcagtat atcctatact ttcacttaac tcttgtgcac
    13381 ttaaatcaag gatttttgaa aacccctgta caaattcagc actttcaagc gctttctgac
    13441 ttatttcgat aattgcattg accgctttgg ttaactcatc aatggtaact ttttgttcca
    13501 tcgaaaaatt atacactgac ccggatagtt ctttaaattt ttcaagtttt gatcgtatat
    13561 cttgattttt tatccctaat tcgttggtaa aatcaacggt gtctttcagt acattaaagg
    13621 tttcaccaat gccttttcga atagctttaa tagcttcagt agctttacta atatactgta
    13681 tttcattggt gatcatttta gcctgatcgg caatggttgc atgaatcccc tttactatgt
    13741 cagtggtttg atctgctaat ttatttactt catcagcaac aactgaaaat cctttaccgg
    13801 attctcctgc tcgtgcagct tctatggcag catttaatgc cagcaggtta attttatcag
    13861 ctatttcatt gatattcaca atggtctgtt caacttcttt taaatattca gctattgatt
    13921 caatagcatt gattgatttg gtaatggtaa cttctccctc ctcagattgt tttacagctt
    13981 gctcaataga atttgataaa cgcgttattc gctgtgacac ctgactgctg ctggcatcta
    14041 ttgattcaat atcattttta atcacgtcca tactgttagc ttgctgttga attgcgttga
    14101 gattggattc aaacgacgat gacatctctt cgtatgcgct ggaggtttct tctacaatgg
    14161 ttgatagttc ccttgcaata tcatataatt tagttgctga aatattaaga tcagagctca
    14221 atcgttttac ttcttctgat tttgcatgtg ttgattggaa caagttcttt aacgattcaa
    14281 ccatcttatt aaaagcgctg gtaatataac caaattcatc gccggactgt accggtatgg
    14341 caacgtcaaa attgccttta tcaacttctt tcaaaccatc agttaactga cgaagaggtt
    14401 taacaaggat gcctgcaaaa aataatcgta tcccaaagcg gataaataca tatactataa
    14461 taacaattgc tattaatatg gcgacagttg aatgcataaa tgaacgatat gcagtataag
    14521 ggaaagctat ctcatgaata atttcattgt ttatgtatcg ataagcaaca acgtgctgac
    14581 catcagttgc aacatggtat gttcgcattc cgggaaattg taccgggatt aagaactgtg
    14641 ttatgtcatg ttttaactct tcaccttctt ttgaagttgt ttcaaggtaa ttggcgatgg
    14701 cactggtaaa ttgttgcaat ataggttctt gttttggtaa taataacttc aggtcattcc
    14761 tgaagttagg gccttttaat tgcgatatct tatggtggaa atgatagacc ttcgcatcaa
    14821 gttccctgca aaatgccatt aatgcactat agtcaatggt gtttttatgc tgctcaatat
    14881 aataccgtaa tgttgccaca tatccctgaa acgatacagg ggtattatcc aataataatt
    14941 gcaacccctc tacagtatta ttttcagcat gtgaaaaaat catattccat gtatatgcat
    15001 gtttcatctc cattgcaatg tcatctttat tataactatc tggcagattt tgtgtccatg
    15061 tattattggc aatattgtat gtagcgtgat agatatagtt atccatagtt tggttgttga
    15121 gtactgttaa caaagcagaa cggtaataaa tttcatcaaa atccttttca ttctttttta
    15181 gaacaataaa tcctataatc tgcagtaagc acaaaacagt taccagtata atcccgatga
    15241 gtttacctaa aaatgaaaat cgttcttttg aattattgat ataaactata aagacaagaa
    15301 agaaacctaa aaggttaaac aatgtccatg ccgtatggaa tacgtctcta tcaataacac
    15361 cttccctgct cagcgtattg gtggtggaag gaacgacggt acctatcact gtgaataaaa
    15421 acaaaagtaa taagatcttt ttttcttctt tattgctggt aaaaaaacgc caccacagta
    15481 caataaaaga acataaaatt ccaaccatta ttaagacacc tatctgcttg cttatctcat
    15541 cagcatcaaa atcataatag tgccctctga ataaaaatac aatatccgac tgcaacgaga
    15601 caatgataaa agcaatcagt gcaccaaatg aaagtatata acccatgata atatatattt
    15661 tagcagcacg gggagctttt ggagatggat aaaagaaata aaagtatccc agatatgcta
    15721 tccccagcaa tatggttaaa atggttagcc atcgatgata tgctgccaat ggatgataaa
    15781 cacttgatga tataaaatat ccaaaattaa aaacacccag tataattaaa gctttagcta
    15841 atatggtggt agcttctgat ttatcttttg tattgtataa aaaaaatcct gttgttaaga
    15901 aaaaaattat aggtataatt gtgcccaatg aaaacgaatt gaacagtata atagaaccca
    15961 taactgccat accccttata tatatttttt tttagtaact ttgtttacat ataataaatc
    16021 ggttaaaata atcaagaata ttttataaaa atataatctg catagactac attttatttt
    16081 taaatgatgc cttgttgaat ctaattctat ttataataat gattattgga tttaattatg
    16141 atttgcaggt tatggcatat ttttcgtttg acaaaaaatt agtgctatta gcttacatat
    16201 aaaccatgtg cagcattgtg ccagcgtagc tcagttggta gagcggcgca ctcgtaatgc
    16261 gcaggtcacc agttcgatcc tggtcgctgg ctcattgatt attaattgca aaaatgattg
    16321 ttgggaaacc ttcgtttaca tgtagtgtta tcaggattat cgcagcatcg aggtaagata
    16381 agccttacac tcttttcaaa gaaaatataa tcccataccc agttgatcat aacaaaaagt
    16441 ttatttctaa atccaattaa atagagtata tggataaata tccagataat ccatgctata
    16501 taccctttta aatgcaaacc agctacctgt gttactgcat ggttgcgtcc aatggtaacc
    16561 atactgcctt tatctttata gtgaaatggt tttgcttttt tatttttagc tattaattgt
    16621 agaagtcgtc caacatattt accctgctga atggctaccg gtgcaatcat aggcaatgtc
    16681 tttccatcct gactatattg tgctaaatct ccaataataa aaacatcatc atatcctgga
    16741 atgtttagat tgtcgttaac ttgtattctc ccgttggtag atattttcag gttacaatca
    16801 ctttttatca tttcaccctt tacccctgct gtccacacaa ctgtttctgt agggattatt
    16861 gggctatcgt tcaggtaaac gccagtagcc tctattttac taacattagc atgggtcaaa
    16921 acgttaacac ctttttttat taattcagac tttgcatact cagataagat tggaggatac
    16981 atgcttaata tcctgtcgcc tgcttctatc aaacaaacac tgatttcttc ataatctaac
    17041 tccggaaaat ctttatgtaa cggcccatga accagctccg ccaatgcacc ggcaaactcg
    17101 acccccgtag gacctccgcc aacaatgaca atggtaagca atcttttccg ctgattctta
    17161 tcatcaataa atgaagctct ttcaaaacag gataaaacgt gattcctgag tatcattgca
    17221 tcatccagtg tttttaatgg aaaggaatac tgtgaagctc cactaatatt gaaatagcta
    17281 ttgacgcttc ctgcagctac caccagataa tcaaatggta tatgttgtcc aaggcattct
    17341 attgttttat ttgcatagtg tatagctgtt acttctccac gtatgaaacg tatatttttt
    17401 gtatttctga tgataaatcg taccggagaa gcaatctgtt caggttctat ttctgcagca
    17461 ctcacctgat acagtaacgg taaaaaagta tggtaattgt ttttatcaat aagaattaca
    17521 ttataattgg tgttcgatag ttcctttgca acccataatc cgccgaaacc agcaccaatg
    17581 ataacaactg ttttttgcct ttgatttatc atggatggca tcctccattt gatataacta
    17641 ataaggatta ataattatat ccctttttat acctcagtct aaaattgtta ctgtgatgtg
    17701 caatctatga aaatcatata ttgcagtatt aggcaagata tcatcatcac tcaatgaaaa
    17761 tataataaat gtaacttttt ttaactatac cttttctttc tggtatttta cactgagttc
    17821 atgccaatgc tatatccgta taagcagtcc ctgtaaacac ttctttcact gttctaaatc
    17881 gtatgaaaat ttctaaaaat gtttttttaa aaaataataa aaattttacc aatgcctgcg
    17941 tctacaaagg gaatcaacaa tataattatt agagatttac ttattgaata aaaaaattgt
    18001 tgacgtgaaa aacaatccag ctagactttc catccatagt aatcaattat taaataattc
    18061 attaacacca tttattcgat aattatagta ttggcatctt taaaattttg tttatttatt
    18121 agttacaata tgataaatct tattctgctt atacctgttt tcacaaacga tcatgtgcaa
    18181 tataactatt atagtataga ttcattttta tatttgacat tacattttac aaagttatat
    18241 taaaaaatta tttttaggta aatgaataaa gcaacaaaaa aaaaattaat aaaaaattaa
    18301 ggtattaaca tgcttaataa taaactgcta ttttatagta taacattttg tataactcta
    18361 ttgctcggtt ttgcattttg tataatcaag ttaatgaata actcattata tgatagtaat
    18421 aatagcctgc ttaaaccggc tgatgttttt gccaataatg atgagtatat agcgtatata
    18481 caaacgctta ctatacccgg agtaaagttt accatacaaa ccatagaaaa gggtgataat
    18541 ttctggaaga tagcaaaaga acacaagatt gacattgata ccttgatagg agccaatcct
    18601 ttatgggaat ctattacagc aaaagttgac cagaagatag ttattccatc tcaaaaaggg
    18661 gttctccatt ttgttgcaga tattaatgaa ataaattctc tccactcact ctatgatgta
    18721 tctgaaaaaa atgtattaac gccatcccta tttttattac gtttgcaatc tattgtatca
    18781 aaaccagaaa aacctattgc cgtatttgtt gtcgatgcac gtcctaaagc agatgttatg
    18841 actccacagc tttcaaagca atttaccata cgagaaatgt ttagatcacc tcttggcgga
    18901 cgattaacat ctttttacgg tggaaggtta catcccattt tcaggcagtg gggcttccat
    18961 aatgggctgg atatagcagc accttatgga actccggttg gagcagcacg gggaggtgtt
    19021 gtaatcagtt gtggctggat gggcggctac ggaaaagcag ttattataca acatgatgaa
    19081 ggttttaaaa cattgtatgg acatttgtca tcaatacacg ttcgccctgg acagcatatt
    19141 aaagcagggc aatttgtagg aagagttggt tcaaccggat ggtctacagg cccacatcta
    19201 cactttacat tatggcaata taataaactt atcaatccta tgaaagtgct atggtaatag
    19261 cattaccatg tatcaaaaac taattaagga atactttgaa aaagcatcac tatattatac
    19321 caaagaaatt ataagccgtt tcaatcaatt tttacatctt ttattacagt acaatacttc
    19381 acacgatctt acccgcatta ccagtattga tgaaattatc attaaacatt ttgtcgattc
    19441 attatttatc acaaaattta tcaccatccc ttcacctctc cttgatattg gaaccggcgc
    19501 aggttttccc ggcattccat tggcaatagt ttatccggat aaacgcttca tattggctga
    19561 aagccggcat aaacgcacac aatttttatc tattgttcaa aattctttgc aactcaataa
    19621 tattgaaata taccctcacc ttgtaaccga aaagtcattt tttgaagtaa gcggtgttat
    19681 tactcgtgca gttgaatcaa tagatgaaac cctgcatcga tgtcatcatt ttttgcaaca
    19741 ggggaatatt gttttattga tgaaagggcc atcagtggat gaagaaaata taactttgtg
    19801 cgatagttat tcaaagataa tggatcaacc gtatacattg cctgccacat catataaaag
    19861 aagacttatt gtttataaaa aaacagatgg aataataaaa aaaacatatt ttattaataa
    19921 agaaggaaga tcaggcgatg gattggcaat aacctccggg gacaataaac gttacaggga
    19981 attaaaaaaa attattactg acccaagaaa atgcagcatg tcgacagttg tcggaaaaaa
    20041 aataatcaaa gagcttgtct cttctcatcc acaattatgt cgctatattg tattgtatga
    20101 tgagtatgct gaaaccgata ctgaactgat gcagataatc gctgattttg gacatcaaag
    20161 aattattcta ttaaaaaaag cacttttcaa cgagattgat gtgctgggca ataatcagcc
    20221 aattctggtt attgatgtgc cacccataac accatggaat atggatactc agggactatc
    20281 gctcatgatt ccttttcagg atccagtcaa cgttggtgcg gctatacgaa gtgccgtagc
    20341 atttggtgtt aacaatatta ttatatgcaa aaatgccgca tatccctttc atcctaaagc
    20401 aatacgttca tcggcaggtg cagtgttcca ctgtacttta tggcagggtc ctccacttta
    20461 tcaacatcaa cctgatttca tagaactatc gtatccaatt atagcattag accttgaagg
    20521 aattaatata tacgatttta cttttccaga agatgcagtt ttacttgctg gtgtagaagg
    20581 acagggattg cacagcataa aaaactgtat ccgtgttacc atacccatac aggtggttga
    20641 gtccctcaat gcaacagtag cattatcaat agcactatac gaatggcata agcaaactac
    20701 tatttcttgc taaatgtatc tttcagttta agataccatt tgcccgcttt catggcaaaa
    20761 tcttcaccac ccgtttcatc catacttaaa tctggtattg caccatcagg accgtacatg
    20821 ccttccatca tttttgctat gtttttccga atcattttac gcatcggtat atgggaaatc
    20881 ataccataca ttgccgcacc accttcatag gctaattcag gatttttcct tactgttgta
    20941 aaagcttctt taacatcatt gataaactgt tgtgcaacct ttgcatgaat aggggttatc
    21001 atagcatgga gagcatcggg acgctggagc ctgtcaacat gccatccctt tttttctaag
    21061 atgtcgccaa tagcaaatgt attcacatca ggctcaatcg aacggtatgc aaaaacactg
    21121 gattcaggtt tgcccactat ttccatacct tttattgatt caatcccctg ctgtattttt
    21181 ctggaagtat ccataattat ttttgcattt tttatgtatc ctttctcacc ctgtgctacc
    21241 attgctgccc aggccgctgc tatagttcca ccaggccttg tgcccagcaa agcaggtgac
    21301 gcaaaaacac caccgggcca attttcataa ataaaaaatt gatggcgcaa ataatcaata
    21361 ctacggtata caatgactga agcaccttta gcactaaatc catatttatg tacatcagca
    21421 ctcattgagg taacaccatt cactctaaaa tcaaatgggg ggatatcata gcctgatttt
    21481 tcaacaaatg gcaataaaaa cccgccaagg catgcatcaa catgcaaagg aatttttttt
    21541 aaatgcgcaa tggaaccaag ttcagcaatt ggatcaacag tgccatacgg atagcatgga
    21601 gctgaaccaa tgataagtat ggtattgcgg tttataagtt tttttacagc attcacatcc
    21661 accttaaaat ttttatccag aggtgcacgt ataattttta catcaaagta ttctgctcct
    21721 ttttcaaaag cgacatggat gctttcagga acaatcattt ccggtttttt aatccacgga
    21781 tgtaatgaac gtgccctatc gcgataggtt ttaacagcaa gcaagcaact ttcagttcca
    21841 cccgatgtca tggtaccaca ggcttctgga cctgcatgca gcatgtttgc agtcattttt
    21901 accacttcat tttcaaactt cttaaggctt ttaaaagcca tgggatttaa cgcattttca
    21961 tcaaagtata gttcaaatgc tttttgcaaa aatttagtgt gtgcatcatc tctatgataa
    22021 acaaggctga atgtttttcc ctgtttccag ttagcatcat ctttccgata ctgtttcatc
    22081 atggacaaaa ccttattgta ttcaataccg tttggtggaa gctgtgttat tgccatgcga
    22141 tacctcctat aataataatt atttattatt attattttac ttatatagaa ctgaaaacat
    22201 atcccaatgt tatgccaaga taattgccaa tagcatagcc aataacaccg gtagtaatac
    22261 cggacaataa aatgtattta ttgtggagtg catcggcaac cactggaaca aatggtggtg
    22321 aacatatgcc tgcaactgaa gtgatgatat aggtatcagt atcaatggaa aaaatcttac
    22381 acaatatacc atgcaccaat aaactcccaa aaattgctaa aaacacataa aacaatagcg
    22441 gtatattaat atgaataata ttatcaacat ttaccatcga accaacaata gtacaaaaaa
    22501 tataaataag atacatccca aactgaaagg ttcgtttaag attatgtaca tacggaataa
    22561 gtgaaagcaa aattgctatt gtcgttactg ccaaaatgac aaccatcgtt tgatattgat
    22621 ttggcaacaa accggcaata cccagtgaaa ttgcaaccac tacaattgat aaaagcaaag
    22681 ctataaccag atttttcaaa atatttttgg aaaagatatc gccatacaat ctaatatctt
    22741 caccagtctt tgcattttta tcttcttcat tttcattatt tattacttta aaaggcggta
    22801 aaaattttaa aaaaagtttt tgtgcgatag ttactaaaaa tataatgtat accattgaaa
    22861 gtacagtgtc ataggtgtga acaattacaa aagttgttga atcaacattg aatgcttctt
    22921 taatggcagc taaatttggt gttccgcctg tatatacccc taaaagcatc ccggcaatct
    22981 tccatgcatc aggaattata tgccttatca gtaaatatcc agcaaatact acaataacta
    23041 ccgcaataca ctccagtgta aacgccagta gtgctttacc ggctaacctg aaccatgttt
    23101 taatatttat tgtgaagagg agcattggca aacctaaaat gatagctatg ttggagctta
    23161 tatcctgtat agactttgcc tgttcaggca atatgcctat atttcctata gtgatgcctg
    23221 cactataaca taacaataca gcacctattt tattaattga tggaaattta tagcatacgt
    23281 aaatgatgag cgccggaatc aaaaggtaaa aacaaatcaa taataaaaag atagcagtat
    23341 tcataccacc tccatttatt tcactatagt gaatattatt tcactttagt gtaattgtca
    23401 actgtttttt gtttgacata tatatcataa agattttttt aataccagat tattaatgac
    23461 gtaaaggaat ataaagttac tgctatgaaa aaagaagaaa gaataaaatg ggataaagaa
    23521 caacgtacag ccagaatcat tgaaatagca caggaattgt tttttaccag gggatttgat
    23581 gcaaccacaa tagatgaaat tgcccattta gcaggatata ataaacgtac tttatatcac
    23641 tatttcaaag ataaagaaga gctgttttta gctgttgcac tgaatggatt aaaattattt
    23701 aatgaaaaat tggaagaggc attcaactat ccattagaag gtcatacaag gttatacagc
    23761 atgggaaagg cattctataa tttttttttg aaatatcctg attattttaa tcttatcatg
    23821 acatacgaag cacgtaattg catgtattat ccggtaacca acagcaatac aatgagcttc
    23881 tataagcttg agtgtcaaaa aattgcagat agtgatactg aattaataat aaaagctatt
    23941 gaatatagta ttaatgacgg aataatcaaa acaacattaa caccaaaaca attaatgctc
    24001 atattatggg ggcaagtttt tggcatcact cagattataa tgatacgaaa agactttttt
    24061 aaagaagcat acggcataga ccatgaagaa ctttttgaaa catttttacg tgaaacggaa
    24121 aaggcattaa aaaattaaaa tgatatagta attaaaaaag ccgccagaag gcggcttttg
    24181 caaagtggtt ttctaaaatg aataattcat tatatcgctt taacggaaac agcccgcgga
    24241 cctttatcgc tgctttcgat ctcgaattca accctttgtc cttcttttaa tgtcctgaaa
    24301 ccgttaccta taataccggt ataatgcaca aaaacatcac taccgtcttc tttggaaata
    24361 aatccaaaac cttttttttc gttgaaccac ttaattgtac ctttgggcat gtgaaaactc
    24421 cttaaaaaat aaaatacggc gtaaagcctt tcagtatgtt gattttgctt ttaacctcta
    24481 aaaaacctca tattgaaatt tcacaagaga caaaaccaaa atacaattca tactattata
    24541 catatatact attacttctt attgttgtca acattttatt acaatttatt taattttttt
    24601 tataaaacct ataattcaca taaaacctat tttattgatt ctttacctat ttggtaaatt
    24661 ttaaaaaaat agcacacatc aaaccatatt gctccatata ttcataaaaa ttttttactt
    24721 gtattttaca gtgattaagc atagctggaa ttcaatattt attgtactgt aatcttgata
    24781 ttatttttga ataatcccca tttttatttt taatatcatt gtttttaaat aattatggca
    24841 aaataaattt tttataattg caaaaaacat agcaattcat atataaaagt attaataatt
    24901 tttagtataa tacgtttgtt aattgtgcga taaataaaca aggatatcta atcgatgcaa
    24961 taattatgag ttttataaaa aatattaagg attaattaca tgagaataat tgctatttca
    25021 aataacaaag gaggtgttgg aaaaacaaca agtacggtaa atattgcagc agcactacaa
    25081 attattggaa aaaaagttct tgtcattgat attgatcatc aggctcagtc aacctatcat
    25141 tttggttata atccacataa tctgacttac tcaatatata atatcctgaa gggtgagatt
    25201 gcgtataaag aaatcttaat tgatagaaat ggtctgcata ttttaccatc taaaaatgat
    25261 ttaaaggata ttgagtttat tccacttcct gcaaaagaat ttctgttaaa ggaagctctg
    25321 gaaaatattt ctggttatga tttcatttta attgattgcc caccctcttt aggaatttta
    25381 actttgaatg cccttgcttt tgcaaaagaa atttttgttc ccttgcaggt tcagttttta
    25441 ccatttcatg gcatgtataa cctttttgaa gcagtgcaga tggtaaaacg cagattaaat
    25501 aaagaaatag aaataacggg tataatagga accatgtata atcctaaaaa gcaaataaat
    25561 gtagaagtaa tagatgaaac aaaaaaacgt ttgcccggta aattgtttga gacattaatt
    25621 cgtgaaaatg tattgttaca ggaagcgcca agctggggaa aaacaatctt tgaatataaa
    25681 ccaaacagca atggagctga tgattatatg aacctgacaa aagaaatatt agaaagaagt
    25741 cccgattaaa atctataaat gtgatttttc aattacccgt ttagcctgtt tttcaagtat
    25801 ttgtaccgcc agaatcaggg tagaaaaacg cttatcttta gtttgaccat gataaaacct
    25861 ataataaatt tgctgggcta taactgctaa cctgaataaa ccaaaacagt agtaataatc
    25921 aaaatcgctc atatctatgc ctgatttttc atggtatcta ttgaccagtt ctttccgtgt
    25981 taatgcacca tccagatggg taggcattaa acgaataagt tgcatatcct ctggatcttc
    26041 tttttccacc cagtatgcaa gtgagttccc taaatccatg acagggtcac caatcgttgc
    26101 catttcccaa tctaatacac caattatttt ttttggattt ttgttatcaa gcaccacatt
    26161 gtcaaattta tagtcgttat gaataatagc tgctctagtc ggcgtttcag gtaatttttc
    26221 tttaagccac gtaattattt cggtatatga tggtgcatca ggggtcagag catcattgta
    26281 acgatttatc catccatgta cctgtcgctc aatataacca tcaggctttc caaaattttc
    26341 cagtcctaac tctttataat gtaaagaatg aagcagacac tgtatatcca gcagctgctc
    26401 acataaatat ttaaattcgt caggtgtata gctcaatccc tgtggtaatt ctttacgcag
    26461 tattattcct ttgattcttt ccataacata aaatggggca ccaataacgg attcatcatc
    26521 acaatatact aatggagctg gacaataagg gaataccggc ttcaatgctt ttaatattac
    26581 aaattcacga tgcatatcat gagctgtttt tgctttttta ccaaaaggag gcctccttaa
    26641 tactaattct ctatcgccaa ttttgataaa atacgtcagg tttgaaaaac cacctggaaa
    26701 ttgttttatt tcaatagttc cttttatatc agaaacattg ttaaccagat aatcaaacag
    26761 tatatgttta tcaatgtcct cgccttcacg aatatctgct ggtttatcta tataattttc
    26821 catacaataa actcctttga aaagaattat taacttctgt cattacgggc ttgactcgta
    26881 atgcaaatat gtaactttaa ttcctaaaac cattttaaag agatgattca ttataacacg
    26941 cacaacattc tcatattgag ggatacagca ttttatgtcg ttctgaaaga gcgataacaa
    27001 ctgaagtatc tcccctttct caaaacaatg agattctcca ctccgttcag aatggcacat
    27061 tgccactttg tcattctgag ggacacagcc tcgtagaatc tattcgttta tacaggagat
    27121 tcttctatac cacctaacat gactcaactt tgccttataa tgaggttcac gatacgcttt
    27181 tatctgctat ggatgccata cacggaataa cagcaactcc tccttctata aataaattat
    27241 ataaaatcta cccgttatgt atatagttat aaataatatt aaaacattcc aatgcatatt
    27301 cttcgggtag tatatttctt cttttatatt tccagcgctt aagataccaa tcctgcatca
    27361 ttgccttagt tactgatgca atcaatgagc tgtttaaatt tgatttgcat tcactttttt
    27421 ttattccatc ttttatgata ctatcaatca atgattctgt aaaaagctca ctttcgatag
    27481 ttttattata ttcaatccct gtaaaatatc gtgtttccat atatgaaaaa taaaaccaat
    27541 ctttcaatgc ttcgcttata aatatatgtg catggataaa attatataat ttatcttttg
    27601 tggatgaagc cttgcttaat gcactcgaca atgttttttc aactgcagct cgtccatact
    27661 gttgaataat atgtaaaagt tcatctttag aaggaaaata agaatagaga gctcctatgc
    27721 ttaacgatga agcacgtgag agctctcgca tactcattgc agcatagcct tttttattac
    27781 ttatactaat agcagtgttt ataatccttt taagattatt gatggattga tcagactttt
    27841 taactttgat aagctgttta ttatcatcat acagtttggt gacaatttca tccagaacga
    27901 ttttaatact cttcctgaaa ttgttgtaat tatcaatgaa taattcattg aaattatctg
    27961 atttctctgc cttcatagcc tgacaatatc ctttttgcca ctgcagtttt atg
//
LOCUS       DAOOJH010000026        25955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1949_length_25955_cov_9.450965, whole genome shotgun sequence.
ACCESSION   DAOOJH010000026 DAOOJH010000000
VERSION     DAOOJH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25955
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_25955_cov_9.450965"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /gene="glmS"
                     /locus_tag="PLJ75_05330"
     CDS             <1..1761
                     /gene="glmS"
                     /locus_tag="PLJ75_05330"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPD04672.1"
                     /translation="EYRGYDSVGIATLIGDELIIQKQKGKVIDLIKRLDYEKFNGCTV
                     GIGHTRWATHGEPNDINAHPHCDCKGDIALVHNGIIENYGAIKKVLIEHGHTIQSETD
                     TEVLVHLIEYYHQTMSLVDAVQKSLTKVEGTYGIVIISKKEPNKMVAARNGSPLILGI
                     GDGEMIVASDASAIIEHTRNVMYVSDGEIVEITKDTVRTFDLRKNELEKHSNKIDWDI
                     AYIEKQGFPHFMLKEIFEQPETIQNAFRGRIVLDEGKTRLDGLRLTEDELNSIKRIIF
                     IACGTSWHAGLIGEYLIEEYARIPVEVEYASEFRYCSPILEKGDLVIVISQSGETADT
                     LAAMREAKARGVRVLGITNVVGSTIARESDGGVYIHAGPEIGVASTKAFTSQITVLIL
                     LTFYLARRRFMTADAGRELLQELIQVPALVQSILGKNDEIKEIATLYQHHRNFLYLGR
                     GIQFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVVFIAIKDEVYQKVI
                     SNMQEVKARKGKIIAIATEGDEEINKFADHVFYVPATVKIASPLLTVIPLQLLAYHIA
                     VLRGCNVDQPRNLAKSVTVE"
     gene            1921..2295
                     /locus_tag="PLJ75_05335"
     CDS             1921..2295
                     /locus_tag="PLJ75_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPD04673.1"
                     /translation="MIVIEDIDYIGVTVSDMDNAISFYSDLFDFEVIERVSNTDEAYI
                     RVGDILLKLVEVKDFKAPENLKNNICFYIDEEDFDDALDELQEKNITIVYGPENIRKG
                     RLVVFTDPDGNKIELRYPRFSL"
     gene            2317..2943
                     /gene="eda"
                     /locus_tag="PLJ75_05340"
     CDS             2317..2943
                     /gene="eda"
                     /locus_tag="PLJ75_05340"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPD04674.1"
                     /translation="MSLQFSIPIIPIGVFYDVARTLKMFELLMQHGIRCIEVTLRTAQ
                     ALDCITALKNTFPDAMIGAGSVITHDDFKAAVDAGAMFIVSPCVSDSLIELSHKNKNI
                     YYIPGFTTPTELAHALQMHVDVLKFFPAEYCGGVHYLKAIVEPFAQFSFSIIPTGGIK
                     PAHIAEYLSLPKVVACGLSYIVDSYLIEHGDYINLEKRINEILACLQR"
     gene            2944..3894
                     /locus_tag="PLJ75_05345"
     CDS             2944..3894
                     /locus_tag="PLJ75_05345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD04675.1"
                     /translation="MSIYKNIETAIYKRENLLKQTITNAFRLVNGQGDGCYGLILDYY
                     DDYLLLQVYDSSLYQEYHGISDHCIKILACCNRAIKGVLLKDRSKVNDPQKIAESRKS
                     VCITGTLPPVPLIAKQNDIVAGVDLINGQNTGLFLDMRGVRQRLAPYYANGGKLCNLF
                     SYTGIFAIHALKNGLHHAVNVDMSKSVLYRAKQNYMLNHIKDDNRDFVAMDCKKFLQY
                     AAKHNDTFHFVIFDPPTFSRNKGHNFSVKKHYNEFLSLIAPIVSDGYILTTINTISIS
                     DDEYISLHPGNWELQFLEHEPDDFPYYKEPYLKAGLWKMR"
     gene            4008..4673
                     /locus_tag="PLJ75_05350"
     CDS             4008..4673
                     /locus_tag="PLJ75_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04676.1"
                     /translation="MVVLYIAVISVIVGVIVVLYSEFFEHTIMSNIPHDRYVPNLDVE
                     MSSHSMHNGKEQYHKTMDSDDIPDIDIDFDSIPEDETTIDTTPARSNPKSEVISIDEQ
                     SQQNNNKNDDVDVVMYVDSSGTIGDIGSGMRSENWSSLTRIGAGTLRYKQQGINIAIN
                     NKLYRYDFYRINNLKVYDNYMLFTLKGKQGVNCLLLNNESDILSFIENEYKQFVESHK
                     QDG"
     gene            4679..5674
                     /locus_tag="PLJ75_05355"
     CDS             4679..5674
                     /locus_tag="PLJ75_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04677.1"
                     /translation="MYYRYKSKQRKRNTTKLIINILLVAIVIYIVHEYHQYIFFWKYT
                     LNKLHYKVEMAMRQNDTIKRQGQLKDLCIAAKKYVESNPSSTDAYYLLGTVYYLYASS
                     ISNIPVYALYDENTLDTIPDEAKQNLINAIKAINKGLAFGGRSSIDDTTRIILASSLY
                     ASRFYPINEIYTIIQKVSNINKLSIDNARLYSLIAIKSGHTEEGLELVKLKGVSDNSE
                     GRMFLAASYLYANQQSNAIIEYRNILKNSMDNAIIKAVHIELGKIFYKKGIYTESLQH
                     LNAALQIDNQDIQCKLWLGKVYSAVGNIAQAKAVWSEVLLLDSENVEAKRLLGIM"
     gene            5803..6828
                     /locus_tag="PLJ75_05360"
     CDS             5803..6828
                     /locus_tag="PLJ75_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD04678.1"
                     /translation="MIFDSVYSLFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFIQKVHKRSTLV
                     RPRVVIGVPSGITEVEKRAVRESAEQAGAREIYLIEEALAAAIGANIPIHEPAGHMIV
                     DIGGGTTEIAVISLGGMVIADSVRIAGDEFDEAIIKYMRTQYNLVIGERMAEDVKFRV
                     GNAFPEKKIETMELKGRDAISGLPRTLEIDSTEIRKALKEPVDQILDAIKHTLERTPP
                     ELAADIVERGIVLSGGGSLLKGLDKYISKETGVPVIRAENPLTCVVMGAGKFLEEIKN
                     LYRSNLK"
     gene            6924..7793
                     /gene="mreC"
                     /locus_tag="PLJ75_05365"
     CDS             6924..7793
                     /gene="mreC"
                     /locus_tag="PLJ75_05365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPD04679.1"
                     /translation="MEFFTRHKALILFIVFTLFCFISLSIQSSRVTNVVEGAGNMAIF
                     PFQKLYHAIQTNISLLWSGFSQLRDAQEELEKTRARLQYYESIAEEVEEIKRENQQLR
                     NLLGLKERLPYDSVLATIISKDPDNWFRTIIVNRGSSDGIKVNMPVVAFSGDEKAIVG
                     KIIEVRGSISRILPIISPDLKIGVVFQESRYPGLLSGYSAIGKMCIVDYVDKTATIKA
                     GDIVITSGQGGVFPQGLLVGVAIRSFPSESGAFQRVLVKPYINFDLIENVIIIKKEPD
                     LEILKLTEEETQQ"
     gene            7790..8290
                     /gene="mreD"
                     /locus_tag="PLJ75_05370"
     CDS             7790..8290
                     /gene="mreD"
                     /locus_tag="PLJ75_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPD04680.1"
                     /translation="MILMYIITGALVLASLIIQGHSSFDVLRIGGVKPDLVFIVIVYF
                     AYSFGSLYGEVTGFIGGILNDAISNSPLGFMAFPKMALGFIIGFFGRSIFKENLFTIF
                     LLIFFASIAKGVITLILAYLFHEGALSSVTSIILPESFYNALLSPPLFFLFDKIFEQE
                     LSREGR"
     gene            8294..10120
                     /gene="mrdA"
                     /locus_tag="PLJ75_05375"
     CDS             8294..10120
                     /gene="mrdA"
                     /locus_tag="PLJ75_05375"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPD04681.1"
                     /translation="MQFASVRTKIIEEFRKRLYVVGIIIICMFGIFFLQLINLQIIKG
                     REFSEKSRMNMESNIPLPASRGEIYDRNFSTDKQNIVLATNRPAFNIIVIPAQYKEKN
                     QLYYAIQNVCALLQLPYEEILTDLKSTNPYNRYIIQEDVPFDIVVTIATNQDKFPNIM
                     WEDAPVRVYPFNNMFSHVIGYIGSLTKEEYNTYRDVGYKYYQKIGKAGIERQYDSILR
                     GVDGYVRRIVDAKNRTEGEEIGQHPKSGYNIVLTLDYDVQKTAYTALGNNRGSVIVMK
                     PATGEIISIVSKPDYDPNLIISKNNIKIITELNNDKNKPFLNRAIQSRYPPASTFKLV
                     TSIAALEEEKWNPAITLYCPGKYTLKGYVDRDFYCYQAHGSVNLLWAIAKSCSVYFYQ
                     LGLKLGPTTIFNYAGYLGLAETSGIDIPGEITGFIPSKKWKLKTFGQPWFDGDTVNLS
                     IGQGFMLVTPIGMMDFLSAIVNSGVVFKPHCIKEIRSIDNTSVVSVVIPEKLREIPLS
                     PTTLDTIKRGMHMAVVNGTAARLGYLKVQMCGKTGTAQTRSKRQEDVSQHAWFVGFGP
                     YDSHPEKVVAIVVMVEYGIAGAATAVPIAEQVFTTLIKKGYF"
     gene            10120..11436
                     /gene="rodA"
                     /locus_tag="PLJ75_05380"
     CDS             10120..11436
                     /gene="rodA"
                     /locus_tag="PLJ75_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPD04682.1"
                     /translation="MFKTIRSSFQEYDIKLLISIIALILIGIITIYATGFDPIEKVNS
                     GLYKKQLLWFIIGIICMFAISRVHYRILGDYSLYIYLVVLFFLIITTIFGAKIRNTRA
                     WINFGFFAIQPSEFMKLATILLLSKYLELRQFDIKRLRELFIPFAIVGVPVLLIYMQP
                     DFGTAMIFIPIFFALLFLGGADILQLISILGVATIALFVPMMLTYIEWSGINISGGFF
                     SFLKGNELLYIISGSLLAVSIISFILYFFFNSKVYRAIYIPSFIISAGLFLSVIIQKF
                     LKEYQKRRILVFLNPDLDPYGSGYNVIQSKIAIGSGGIIGKGFLKGSQSQLGFLPEKS
                     TDFIFSVISEEWGFIGAVIVLLLFGYILYKGCMIALKTSDKFGMLLAGGITCMIFFHV
                     FINIGMALGIMPVTGLPLTFLSYGGSNLIMSLLSIGILINIDSRTR"
     gene            11542..11997
                     /locus_tag="PLJ75_05385"
     CDS             11542..11997
                     /locus_tag="PLJ75_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04683.1"
                     /translation="MLIVLFIVLQISFSFHIYSLVLYVLRRENKYLKGFINTTISNIL
                     LAGGITALAIIHPQNVKEVDFKLLLWFMTGFIMLIMLFIKISIARAIFIRTKDPQNYH
                     FNYFGKKVLHSTVVRFEEILIFFFTMPFFLFCGAYFIARLFNLLLYKHL"
     gene            12124..12366
                     /locus_tag="PLJ75_05390"
     CDS             12124..12366
                     /locus_tag="PLJ75_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04684.1"
                     /translation="MGLITDKIRENAEKCKSCAFLKIRNAEIDKNSVLYDEAMMTLEG
                     VYRASCTMCPFEKDFEKIYGMKPYEYFPMRIAKKEA"
     gene            12383..13060
                     /gene="queC"
                     /locus_tag="PLJ75_05395"
     CDS             12383..13060
                     /gene="queC"
                     /locus_tag="PLJ75_05395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPD04685.1"
                     /translation="MSKKKCVVLLSGGLDSATVAVFAKSRGFDIYALTFEYGQRHEIE
                     ILYAKKIAHYLNINAHDIVHLPDDIFKISSLVLSSGKEIPITRGSAIPSTYVPARNII
                     FLSIALAYAETVEATHIFIGVNAVDYSGYPDCRPQFIEAFQKMVMVGTKSGVEGNPII
                     IEAPLINMTKAEIIALGTSLGVDYSLTSSCYNPSPEGKPCGICDSCMLRKKGFEQAGY
                     EDPAIQR"
     gene            13035..13799
                     /locus_tag="PLJ75_05400"
     CDS             13035..13799
                     /locus_tag="PLJ75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPD04686.1"
                     /translation="MKILPYNGKTAKNMKQSYINILLNTLMFDVLFPAFCSNCGKMIS
                     NINNGLCNNCYDNLNLSVYNYSSQYFFNVEEKIHGKRYWYIDDILLVFKYRNEVSKLI
                     YDMKFKNNKSIALLFSKFLLQGINSLAWDFDLITYIPVSKKRLWERGFNQSELMARHI
                     GKMLHKDVFNLMTIKNENNTQKKLSQNDRFISTYQKFDILSNCTNIVNKSVLIVDDVY
                     TTGATINECARILKMNSAKSVYASILAYAMLKDRDV"
     gene            14004..14330
                     /locus_tag="PLJ75_05405"
     CDS             14004..14330
                     /locus_tag="PLJ75_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04687.1"
                     /translation="MKIKRDDNLAILTLDGRIDQEMSEDLENALQKLIDDGCNNICMD
                     MTDVKHICSSALGVIVAFKRKIKNEGDIKLVITNENLLKLFQTTMLDKVFEIFESQRE
                     CLNAFD"
     gene            14352..15332
                     /locus_tag="PLJ75_05410"
     CDS             14352..15332
                     /locus_tag="PLJ75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPD04688.1"
                     /translation="MTPRKDIHDILKPIEHYLMAVDETVKSKLYTGIDLLDDSSMHTF
                     KKSGKKIRASMIILSGGLNNTIPDEIIDIACAAEIIHAASLVHDDIIDNADLRRGLPT
                     VSRKYGPKVAVLAGDYMYTKALEIAVGNNRTDLFPIMVDATVEMVKGELYQIQYSDID
                     NITIDNYYRIIEMKTARFMAACAKLGAAVAKMNTQQSDVLYECGLQLGYAFQITDDAM
                     DYLNAADITGKDAGNDFINGKITLPLLHLLEVADNKERALLKNYAKTPDNDNWNYVVS
                     KAKEYNVVDYCISIASDYVKKAIEIISMFPDNQYKNILTELALFFVDRKY"
     gene            15387..15608
                     /locus_tag="PLJ75_05415"
     CDS             15387..15608
                     /locus_tag="PLJ75_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04689.1"
                     /translation="MITKEKEEVLRLYDAGLEAYKKRKWDEAIDYFDKALKVDPNDGP
                     SKVYLERARQYKQTPPSDDWDGVFIMTTK"
     gene            15711..16133
                     /locus_tag="PLJ75_05420"
     CDS             15711..16133
                     /locus_tag="PLJ75_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD04690.1"
                     /translation="MAKKVIQVNKNPVYEIGMIATVFGKDTEFYGDLTFEKSLQINGY
                     FEGEISSGDFLVIGEKATVKANIKASTVIIRGTVYGNIEAKDKIEIQTEGKLFGNIRT
                     SKLIIADGVVFEGKCEMIKQPIAVQKSKQVHKESTLVE"
     gene            16175..17626
                     /locus_tag="PLJ75_05425"
     CDS             16175..17626
                     /locus_tag="PLJ75_05425"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015148736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPD04691.1"
                     /translation="MKEFVKKIDNCLYEIPEDYKKGMRVPARIYASYELLHHMDDAVF
                     EQLINVAMLPGIQKYALCMPDGHSGYGFPIGGVAAVDSDDGVISPGGIGFDINCGVRF
                     VITSVSIEEFAPKLNQIMDILFSRIPSGIGGKGIVSLSDNQFDEAMIKGARWAIENGY
                     GVKEDLLFCEENGCIGNANPDVVSGRARERGREQVGSLGSGNHYLEFQVIRNEDVLQT
                     TIAKKIGIIGNNQVSCMIHCGSRGFGHQIATDYLDKFIGVMKQKYHIDIPDRELACAP
                     FHSKEGQAYFGAMNCAINIAFLNRQLIYHVVRDTLCQVFKKSAEELGIALIYDVCHNT
                     AKLEQYQINGNNKKLLVHRKGATRAFTKGMKGIPDTYKEIGQPVLVGGSMQTASYILI
                     GGDRASDAFYSTVHGSGRIMSRQQAKKQFKGRELADSMAKSGIAIKTRSLSGLAEEAG
                     AAYKNIDDVIDTITAAGLSIPVARMIPVGSIKG"
     gene            17635..18075
                     /locus_tag="PLJ75_05430"
     CDS             17635..18075
                     /locus_tag="PLJ75_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPD04692.1"
                     /translation="MMESEEGFEFVENATLADIAIYCKAFTLEKLYAFNAEAFLHICL
                     ENPNDLNAKIDYRFSLQHQSLDLLLVKFLNELIFIKDTQGIVCKPVHVEIEYGQEGSM
                     CTAQLIGDYIINYRINVDPKAATLHKARCEKTNLIWESFIIIDV"
     gene            18096..18983
                     /gene="folD"
                     /locus_tag="PLJ75_05435"
     CDS             18096..18983
                     /gene="folD"
                     /locus_tag="PLJ75_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPD04693.1"
                     /translation="MAKIIDGKAIANEIKQEIKKDVAKLEQEYGKKPTLAVILVGNDS
                     ASEVYVRMKKKASEELGINSLQITLDERTTQSELLKLIHELNSDTNVNGILVQLPLPS
                     HIDENIIINTIDPLKDIDGFHPINLGKLVIGSDDCFVSCTPYGCQELIVRNVPDLKGK
                     HLVIVGRSNIVGKPLANIMLQKNGRANCIVTICHTATSDIGYYTRQADILVVAAGKAN
                     MITKDMVKPGAVVIDVGINRIDDPRNPGKTKLVGDVKFDEVSEIASAITPVPGGVGPM
                     TIVMLMKNTVKAFRLQNKI"
     gene            complement(19077..20306)
                     /locus_tag="PLJ75_05440"
     CDS             complement(19077..20306)
                     /locus_tag="PLJ75_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04694.1"
                     /translation="MSKVINFLKDEWNSYSRNIQISLYVLFALVALFVIFGIQILFWG
                     LGYTDMNNRFAMGLWIIGDLGFVALGGGAFFTGFLLYIFRVDKLQPLINSTVLIGFMC
                     YLFTFILLVFDIGQPLRAWFGYAYPNWGPHAMPQSMLTEVVWCLTFYFIVLCIELFPI
                     ILSNKYLDRYPAVHYFAHYLHKLMWIFAAIGTFLSFFHQGSLGGGMWGVLYAKPYWFR
                     HHYFYLAIVAAIAGGPCLMMLVPKIASWVAKKELVPRDAYMTLAKVAGVTFIAYFLFR
                     IYDIYSLWTHFAPLFDRSFIEMMGGYYGWWVVTLELLASLTAIVILNVKKFRENEQYF
                     IAGLISGVFAIVMLKVDVLLHGFSIPNFSWEGFFTYNPTIQEWSIFFGSIACMILIYM
                     WFTKYIPLFPEKHTEAH"
     gene            complement(20330..21160)
                     /locus_tag="PLJ75_05445"
     CDS             complement(20330..21160)
                     /locus_tag="PLJ75_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPD04695.1"
                     /translation="MAKLTRRWGMVIDLDKCTGCGACNIACSQENNLPIFKDDSDIPK
                     RVTFLDLVEVTNDKDPKAQYPDVKVAYLPKMCQQCSGNDPNHPNPPCVSVCPVVATDV
                     GEDGVVSQIWSRCIGCRYCQVACPYEARVFHWWKPRYEGSFKKSLNPDVSIASRGTMV
                     KCTFCSHIWKRERDRAIANGVTDINAVTYTPACASACPTNAIIFGDLNDPSSPVYEAA
                     VQKNPRAVRLVHSIDELRTKDPSEYEKRLKIKDYPNPKVYYLTSQQWLRDKISGFKNS
                     "
     gene            complement(21174..23144)
                     /locus_tag="PLJ75_05450"
     CDS             complement(21174..23144)
                     /locus_tag="PLJ75_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPD04696.1"
                     /translation="MKLERKDFLTMIGGAVVGTGVGTLISPGPWMGLQWLVEWTQDQY
                     RPPKGEEKYLASICDRCPDKCTIQVRKIGNRAVKIETSNQGCAVGQLLLQVLYHPQRV
                     TTPLKRTGKKGKIDFAPVSWDEAIADISKKINDAINNNKQEQIIAINPAYPDATSMLT
                     ERIALFSGSPYVFQESGYWALSNACSEIAFKTDGAIDYDFENADCVVSFGARLFEGWG
                     NPGRMHNVLSLWKKKGIKFIQIDSIATRTASLADQWVAIKPGSEPLFAMGVAHYLMKY
                     GKRSGATNFGRFTELLINKYTPEKVSELTGVSLDTIESVAKALISAQRPVAVAGRGGK
                     VNSSSVAEFAIIQTINSMIGSMGSSVKLVSFTTPAKPVIPKKAKGLDEYIASDSVCDI
                     ALINNANPVYSSVYGQRLSDKLKKASTVVLCTSLLNDTALYADYVFPTLTSLELPVAG
                     NAVVTPTSEAKHAGDSLLAIAKNIAKISSQFSWKNYLETVQTVKYQTKNIANVDIPVD
                     LIITKLEETEKILSASGSYSIAMIPFEIPTVSDGSGLSLPYVLKGLPGTILKGDTSWV
                     LMNPATASKEGISEGSKIDIESSRGELDTVRVHLTKRIAYDTVAIPIGFGHEEFTDYA
                     SEKGVNPKIIMSDAIDPASGYADWWLTRVKLS"
     gene            complement(23193..23612)
                     /locus_tag="PLJ75_05455"
     CDS             complement(23193..23612)
                     /locus_tag="PLJ75_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPD04697.1"
                     /translation="MNVQAKKLLPFNHQTHTTQYGADCELCHSYDANGRFNGIPTVAV
                     CKQCHDPNKVNNAVFFAGFNDTDKPWETFAQQPDLVYFSHIAVTKNEQKVQCISCHGD
                     KGASKTTAKIKGKMAMGVCMDCHTVTNVSNSCTVCHD"
     gene            complement(23898..>25955)
                     /locus_tag="PLJ75_05460"
     CDS             complement(23898..>25955)
                     /locus_tag="PLJ75_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPD04698.1"
                     /translation="VYNAFKKQIKGLVDGGVDCIAIETMTDLQEARLALLAAKAVTNI
                     PIICSMTFEDNGKTVTGTDIVTAFATLSQMGADVVGANCSMGPDGLVQLFTGAIAQLK
                     QLGVPLSVWANAGLPTLVNGKATYSLSPQQFAMLSTQFATMGFSIIGGCCGTTPAHIN
                     ALANAVKEIRIDIPRSKTFHYITSRYGYIDVQQHQGLIIIGERLNPTARKQFAQELKE
                     NKFSFLREESKKQQDEGAHILDINVGVPGIDESIMMTKAVTILSKTVTIPLMIDSDNH
                     AVIQAALWHYPGIPILNSISAKEESFNSMLPLLQQSGAFVIAMCLDESGIHKEAKKRI
                     AIGQRLITLLQDNGIELSRIFIDPLILAESAESGAAMETLKVIEYFSKHGIKTSIGLS
                     NISFGLPQRKYINNAFLKLAIQKGLTAAIVNPSAIDSNTEKPFTAEETMALDFLEGRD
                     KNAVHYIAHFGTSQQQSRIQSPVKEKSSDILNRIFSLVVEGNIDDIEHAIHQALTMYE
                     PEIIMSNALIAALEKVGQLYSSGEYFLPQMIASADTMKKGFTVLKPLMQQHKESLLGK
                     VLICTVRGDIHDIGKNIVAMMLENHGFDIIDLGKDVPNEAVIASIIEHKPDIVCLSSL
                     LTTTMPNMEQIATMIKHQQLPCKVMVGGAVVTKEYAESIGAYYGKDAVDAVSIAKTIV
                     NVK"
BASE COUNT         8485 a         4106 c         5063 g         8301 t
ORIGIN      
        1 gaatatcgtg gttatgactc agtagggata gctacgttga taggagatga attgataatt
       61 cagaagcaaa agggtaaggt tattgatctt attaaaagat tagattatga aaaatttaat
      121 ggatgtacag ttggtatagg gcacacacga tgggcaacac atggtgagcc aaatgatatt
      181 aatgcccatc cacactgtga ctgtaaaggt gatatcgcgt tggttcacaa tggaatcata
      241 gaaaattatg gtgctataaa aaaagtactc attgaacatg gtcacactat tcaaagtgaa
      301 acagataccg aagttttagt acatctcatt gaatactatc accaaaccat gtcacttgtt
      361 gatgcagtac agaaatcatt aaccaaggtt gaaggtacct atggtattgt tattatatca
      421 aaaaaagaac ccaacaaaat ggtagctgcc aggaatggga gtccgctgat acttggtatt
      481 ggcgatggcg aaatgatagt ggcttctgat gccagtgcaa tcattgaaca cacacggaat
      541 gtcatgtatg tttcagatgg tgaaatagta gaaataacaa aagatactgt acgaacattt
      601 gatctacgaa aaaatgaatt ggaaaagcac agcaataaaa ttgattggga tattgcatat
      661 atagaaaaac agggatttcc ccattttatg ctaaaggaaa tatttgagca acccgagaca
      721 attcagaatg cgttcagggg acggatagta ttggatgagg gtaaaacccg tttggatgga
      781 ttacggctta ctgaagatga gttgaatagt ataaaacgta ttatttttat agcctgtggt
      841 acctcatggc atgcaggact tattggagag tatttaatag aagaatatgc ccgaatacct
      901 gttgaggttg aatatgcctc agaattcaga tattgcagcc caatccttga aaaaggtgat
      961 cttgttattg ttataagtca atcaggtgaa acggctgata ccctggcagc gatgcgtgaa
     1021 gctaaggcac gtggtgtcag ggtgctgggc attaccaatg ttgtaggaag taccatagct
     1081 cgtgaaagtg atggaggggt gtacatacat gcagggcctg aaataggtgt tgcatctact
     1141 aaagcgttta cttcgcagat aactgtttta atacttttaa cattttattt agcccgccgc
     1201 agatttatga ctgctgatgc aggccgtgaa ttattacagg agttgataca ggtacctgca
     1261 cttgtacaat caatattagg aaaaaatgat gaaattaaag aaatagcaac attatatcaa
     1321 catcaccgca attttcttta tctgggaaga ggtatacagt ttcctgttgc tcttgaaggt
     1381 gcattgaagt taaaagaaat atcctatatt catgcagaag gatatcctgc tgctgagatg
     1441 aaacatggac ctattgcact tatcgatgaa aatatgccgg ttgtctttat tgctataaaa
     1501 gatgaggtgt atcaaaaagt tataagcaat atgcaggagg taaaagcacg aaaaggcaaa
     1561 attattgcta ttgccaccga gggtgatgaa gagataaata agtttgccga tcatgttttc
     1621 tatgtgccgg ctactgtaaa aattgcatcc ccacttttaa cggtgattcc actacagttg
     1681 ttagcatacc acattgcggt attgcgcggg tgcaatgtag atcagccaag aaatctggca
     1741 aagagtgtta cggtagagta aactgttaaa taaattttta tgcactgtgg tttaaataat
     1801 tgacaggatg taaacaaatt ttataagtga tagcaggaag tgtttatgga gtaatgagca
     1861 tatatattta atgtgatgtg aaaatacagt aaaaaattat gataataagg agaattcgaa
     1921 atgatagtaa ttgaagatat tgattatatt ggtgttacgg tatccgacat ggataatgca
     1981 atcagttttt acagtgattt gtttgacttt gaggtaattg agcgtgtaag caataccgat
     2041 gaagcatata tacgtgttgg tgatatttta ttaaagcttg ttgaagtaaa agattttaaa
     2101 gcgcccgaaa atttgaaaaa taatatctgt ttctatatcg atgaagaaga ttttgatgat
     2161 gctctggatg aattacagga aaaaaatatc acaatagttt acggacctga aaatatccgg
     2221 aaagggcgtc tggttgtatt tactgatcct gatggaaata aaattgaatt gcgatacccc
     2281 cgattttctt tataaaatta attaaaatta ttaatcatgt cattacagtt tagcattcct
     2341 attatcccca ttggcgtttt ttatgatgtt gccagaacgc tcaagatgtt tgagctttta
     2401 atgcaacacg gtatacgttg tattgaagtt acattacgaa cggcgcaggc gcttgattgc
     2461 attactgctc taaaaaatac atttcctgat gcaatgattg gtgctggcag tgtcataact
     2521 catgacgatt ttaaggcagc agtggatgct ggagcaatgt ttattgtttc accatgtgta
     2581 agtgattctt taatagaact atcgcacaaa aataaaaata tatattatat acctggtttt
     2641 acaactccta ccgaattagc tcatgcactg caaatgcacg ttgatgtttt gaaatttttt
     2701 cctgctgagt attgtggtgg cgtgcactac ctcaaagcta ttgttgaacc gtttgctcaa
     2761 ttttcatttt caatcattcc aacgggtggt attaaacctg ctcatattgc tgagtattta
     2821 tcgttgccaa aggtagttgc atgtggtttg tcatatattg tcgatagtta cctgatagaa
     2881 catggcgatt atataaatct ggaaaaacgt ataaacgaaa tccttgcctg tttgcagcga
     2941 tagatgtcca tctataaaaa cattgaaaca gcaatttata aacgggaaaa cctgttgaag
     3001 caaactatca ccaatgcgtt ccgtctggtt aatggacagg gtgatggttg ctatggactg
     3061 atacttgatt actatgatga ttatctttta ttacaggtgt acgatagttc cctgtatcag
     3121 gaatatcatg ggataagcga tcattgtata aaaatattgg catgttgcaa tagagcaata
     3181 aagggtgttc tgttaaaaga taggagtaaa gttaatgatc ctcaaaaaat tgcagaatca
     3241 cgaaaaagcg tgtgtataac aggaacactg ccaccggtac ctttgatagc aaaacaaaat
     3301 gacattgttg caggtgttga tctgattaat ggccagaata cagggctttt tcttgatatg
     3361 cgcggagtgc gacagcgttt agcaccatat tatgcaaacg gtggaaagct ctgtaatctt
     3421 tttagctata ccggtatatt tgcgatccat gcattgaaaa atggattaca ccatgcagtc
     3481 aatgttgata tgtcgaaaag cgtgttatac agggctaaac aaaattatat gctcaatcac
     3541 atcaaggatg ataatagaga ctttgttgct atggattgca aaaaattttt acaatatgct
     3601 gcaaaacata atgatacatt tcattttgtg atttttgatc cacctacatt ttccagaaat
     3661 aaaggacaca atttcagtgt aaaaaaacat tataacgaat ttttatcgtt gattgctccc
     3721 attgtttcag atgggtacat ccttaccact atcaatacca tttcaatttc agatgatgag
     3781 tatatatcat tacatcccgg taactgggaa ctgcaatttt tagaacatga gcctgatgac
     3841 ttcccctatt ataaggaacc ctacctgaaa gcaggtttat ggaaaatgcg gtaatttttt
     3901 tgaattaatc cttgacttgt aaaatttatc tttcgacaat aatgatgggt ggttttcttt
     3961 attaatcatt catgttgcaa ttccagtgaa ctatacagga tagtattatg gttgttttat
     4021 acattgcggt aatcagtgtc atagtgggtg tcatagttgt actttattcg gaattttttg
     4081 agcataccat tatgtccaat atccctcatg accgatatgt gcctaatctt gatgttgaaa
     4141 tgtcatcaca ctccatgcac aatggcaagg agcaatacca taaaacaatg gattctgatg
     4201 acatacctga cattgatata gactttgatt ctataccaga agatgaaaca acgattgata
     4261 ctacacctgc aaggagcaat ccaaaatcag aagttatttc tatagatgaa caatcacagc
     4321 aaaataacaa caagaatgat gatgttgatg ttgttatgta tgtcgatagt agcggaacta
     4381 ttggggatat tggaagcggt atgcgttcag aaaattggag ttcattaaca aggataggag
     4441 ccgggacttt gcgatataaa cagcagggca tcaatattgc tattaataat aaactctatc
     4501 gctatgactt ctatagaata aataatttaa aagtatatga taactatatg ctttttacgt
     4561 taaaaggtaa acagggtgtt aattgtttgc ttctcaacaa tgaatccgat attttatcat
     4621 ttattgaaaa tgagtataaa cagtttgttg aatctcacaa acaggatggt taacaagtat
     4681 gtattatcga tacaaatcaa aacagcgaaa aagaaataca acaaaattaa ttattaatat
     4741 ccttttagtg gcgatagtta tttatattgt gcatgaatac catcaataca tctttttctg
     4801 gaaatacaca ttaaataaat tgcattataa agttgagatg gcaatgagac aaaatgatac
     4861 tataaagcga cagggacaat tgaaagattt atgtatagct gcaaaaaagt atgttgagag
     4921 taatccttca agtactgatg cttattatct gttgggaacg gtttattatt tatatgcatc
     4981 ctctatcagc aatattcctg tatatgcatt atatgatgaa aatactctgg atacaatccc
     5041 tgatgaagca aaacaaaatt tgatcaatgc tattaaagca attaataaag gattagcttt
     5101 tggtggcaga tcatcgatag atgatactac aaggattatt cttgcctcat cactttatgc
     5161 aagcaggttt tatcctatta atgagatata tactattatt caaaaagtat caaatataaa
     5221 taaactatcg atagacaatg ctcgattgta ttcattgata gctatcaaaa gtggtcatac
     5281 tgaagaaggg cttgaacttg ttaaattaaa aggtgtctct gacaatagtg aaggaaggat
     5341 gtttttagct gcatcatact tatatgctaa tcagcagagc aatgctatta ttgagtacag
     5401 gaatattttg aaaaattcaa tggataacgc cattattaaa gctgtacata tagaattagg
     5461 gaagatattt tataaaaaag gcatatatac agaatcactg caacatctca atgctgcatt
     5521 gcagatagat aatcaagata tacagtgtaa actgtggtta ggtaaagttt attctgcagt
     5581 aggaaatatt gctcaggcaa aagcagtgtg gagcgaagta ttgctccttg attctgagaa
     5641 tgttgaagca aaacggttat tgggaattat gtaaaaattc attttttatc tgctattaaa
     5701 aaaattcatg ataatagctt gacaaaaaaa atagtaaagt atcattcaaa tgcaaatagt
     5761 tttactgtta atcaatattt tgactaaaaa ggtaaacata ttatgatctt tgattctgtt
     5821 tatagcttat tttctaatga tatggggatt gatttaggta ctgctaatac attggttcat
     5881 gtaaaagggc agggtattgt attaagtgaa ccatcggttg tggctgttca aacaagtacc
     5941 ggtaaagtgc tggcagttgg ccatgaggca aaaagaatgc tgggaagaac tcctggcgat
     6001 attgtagcaa tacgtcctat gaaggatggc gtcatagctg attttgaaac tgttgagaaa
     6061 atgatacgat attttatcca gaaagttcac aaacgatcaa cattggttag accacgagtg
     6121 gtaattggtg ttccctctgg tatcaccgaa gtggaaaagc gtgcggtacg agagtcggcg
     6181 gagcaggcag gtgcaaggga aatatatctc atagaagaag cattggcggc tgccattggc
     6241 gcaaatatac caatacatga gcccgcagga catatgatcg ttgatattgg aggtgggaca
     6301 actgaaatag cagttatttc attgggtggt atggttattg ctgattccgt aaggattgca
     6361 ggtgatgaat ttgatgaagc catcataaaa tatatgagga cacagtataa ccttgtaatt
     6421 ggtgaacgca tggctgagga tgttaagttc agggttggta atgcattccc ggaaaagaag
     6481 attgaaacaa tggaattgaa agggcgtgac gcaatatcag gtttgcccag aacactggaa
     6541 attgattcta ctgagattag aaaagcgctc aaagagccag ttgaccagat tctggatgct
     6601 atcaaacata cattggaacg aacccctcct gaattagctg ctgacatagt tgaacgtggt
     6661 atagtattat ccggtggcgg ctcactgcta aaaggtctgg ataaatacat tagtaaagaa
     6721 acgggagttc ctgttataag ggctgaaaat ccacttacct gtgttgtgat gggcgctggg
     6781 aaattccttg aagagattaa aaatctctat agatcaaatt tgaaataaat tataatagtg
     6841 taatttatta tcgtaattat cattaaaaat gctcttgcag attatgcagg agtatttatt
     6901 taataaaata aattaggtac catgtggaat tctttacacg acataaagcg ttgatacttt
     6961 ttatagtatt tacattattt tgctttattt ccctctctat tcaatcgtca cgagtaacca
     7021 atgtggttga aggggcggga aatatggcaa tttttccatt tcagaaatta tatcatgcaa
     7081 ttcagaccaa tatttcacta ttatggtcag gattttccca attgagggat gcacaggaag
     7141 aattggaaaa aacacgggca cggttgcaat attatgaatc gattgctgaa gaagttgaag
     7201 aaataaaaag agaaaatcaa caattaagaa atctcctggg tcttaaagaa cgattaccgt
     7261 atgactcggt tttagctacc ataatatcaa aagatcctga taattggttt agaacaatca
     7321 ttgtaaatag aggaagctct gatggtatta aggtaaatat gcctgttgta gctttttctg
     7381 gtgatgagaa agctatagtt gggaagataa tagaagtaag aggttcaatc tcaagaatat
     7441 tgccaattat ttcacccgat ttaaagattg gagttgtttt tcaagaaagt cgttaccccg
     7501 gacttttgag tggttattct gcaataggta aaatgtgcat cgtagattat gtggataaaa
     7561 cagcaactat taaggcaggg gatattgtca ttacctccgg tcagggaggt gtattcccgc
     7621 aaggactatt agtgggagtg gcaatacgat cattcccttc tgaatcaggt gccttccagc
     7681 gtgtacttgt aaaaccatat attaattttg accttattga aaatgttata attataaaaa
     7741 aagaaccgga cctcgaaatt ttaaaactaa ctgaggaaga aacacaacaa tgatattaat
     7801 gtatattata acaggagcat tagtattagc atcattgata atacaggggc attcttcatt
     7861 tgatgtttta cgtataggag gtgttaaacc tgatttggtt tttattgtca ttgtgtattt
     7921 tgcctatagt tttggttctt tatatggtga ggtaaccgga tttattggtg gtatactaaa
     7981 tgatgctata tccaattctc ctctgggatt tatggcattt ccaaaaatgg cattagggtt
     8041 tataattggc ttttttggga ggtcgatctt taaagaaaat ttatttacaa tatttctatt
     8101 gatctttttt gcttctattg ccaaaggagt aataacttta atattagcct atctttttca
     8161 tgaaggggca ttgtcatctg ttacatcaat aattttaccc gagtcgtttt ataatgcatt
     8221 actatctcct ccattatttt ttttatttga taaaattttt gagcaggagt tgtccaggga
     8281 aggccgatag attatgcaat ttgcatccgt tagaacaaaa atcatagaag aatttagaaa
     8341 gcgcttatat gttgtaggta ttattattat ctgcatgttt ggaatatttt ttttgcaact
     8401 tattaattta cagattatta agggcaggga attttcagaa aagtcaagaa tgaatatgga
     8461 aagtaatatt ccattgcctg cttcaagagg agaaatttac gatagaaatt tttctactga
     8521 taaacagaac attgttttgg caacaaatcg ccctgctttt aatattattg taatccctgc
     8581 tcagtataaa gaaaaaaatc aattatatta tgctatccag aatgtatgtg ctcttttaca
     8641 attgccatat gaagaaatcc tgacagattt aaagtcaact aatccctaca acagatatat
     8701 cattcaggaa gatgtcccgt ttgatattgt agtaactatc gccacaaatc aggataaatt
     8761 tcctaacata atgtgggaag atgcaccggt gagggtatat ccattcaata atatgttttc
     8821 gcacgtaata ggttatattg gaagtttaac aaaagaagaa tacaatacat acagggatgt
     8881 tggatacaaa tactatcaaa aaattggaaa agcaggtatt gaacggcagt atgattcaat
     8941 tttgcgaggt gttgatggat atgtccgtag aatagtcgat gccaaaaata gaaccgaagg
     9001 tgaagaaatt ggacagcatc caaagtcagg ttataatatt gttttaaccc tcgattatga
     9061 tgtacagaaa actgcatata cagcactggg gaataacagg ggttcagtta ttgttatgaa
     9121 accggctacc ggtgaaataa taagtatagt tagcaaaccg gattacgatc caaatcttat
     9181 catctcaaaa aataatataa agataatcac agaattaaat aatgataaaa ataaaccttt
     9241 tctgaacagg gctattcaat cgcgatatcc tcctgcatca acatttaaat tagtaacttc
     9301 tattgcagct ctggaggaag aaaagtggaa tcccgcaatt actctgtact gcccgggaaa
     9361 gtatacttta aagggatatg ttgacagaga cttttattgt tatcaagcac atggatcagt
     9421 aaatttatta tgggctatag caaaatcatg cagtgtttat ttttatcaat taggattaaa
     9481 attagggcca acaaccatat ttaattatgc aggttattta ggtcttgcag aaacttcagg
     9541 tatagatata ccgggtgaaa tcaccggatt tataccatca aaaaaatgga aattgaaaac
     9601 ttttgggcag ccatggtttg atggtgatac cgttaatctg tccattggtc agggatttat
     9661 gctggtaact cctatcggca tgatggattt tttatctgca attgttaatt cgggtgttgt
     9721 tttcaaacca cattgtatta aagaaattag atccatcgat aatacatcag ttgtttcggt
     9781 agttatacca gaaaaattac gagagattcc attatcacca acaacgcttg atactattaa
     9841 gcggggtatg cacatggcag ttgtcaatgg tactgctgcg cgattgggat atctgaaagt
     9901 acagatgtgt ggtaaaacag gaacagctca aacacgttca aaacgccagg aagatgttag
     9961 tcagcatgca tggtttgtag ggtttggtcc ttatgatagc catccggaaa aggtggtggc
    10021 gatagttgtt atggtagaat atggtattgc tggtgctgct acagccgtgc ctatagctga
    10081 acaggttttt acaactctga ttaagaaagg atacttttaa tgtttaaaac catacgatct
    10141 tcttttcagg aatatgatat aaagctttta atttcaatca tagctttgat tttgataggc
    10201 ataataacga tttatgctac aggttttgac ccaattgaga aggtaaatag tggtttatat
    10261 aaaaaacagc ttttatggtt tataataggc attatctgta tgtttgcaat ttcaagagtt
    10321 cactatagga ttttaggtga ttattctctt tatatttatc tggtggttct atttttcctt
    10381 ataattacaa caatttttgg tgcaaaaata cgtaatacac gtgcgtggat taattttggt
    10441 ttttttgcca ttcaaccatc tgaatttatg aaattagcca ctattttgtt attatcaaaa
    10501 tatcttgaat tacgacaatt tgacattaag cgtttgcgtg aattgtttat accttttgca
    10561 attgttggag ttccggtgct gcttatttat atgcagcctg attttggaac tgcaatgata
    10621 tttattccca ttttttttgc attactcttt ttaggtggag cagatatact tcagcttata
    10681 tcaatattgg gtgtagcaac tatagcatta tttgttccca tgatgcttac ctatattgaa
    10741 tggagtggca ttaatatttc aggaggtttt ttttcatttt taaaagggaa tgaattatta
    10801 tatattatat ctggttcttt attagcggtt tctattataa gtttcattct ttatttcttt
    10861 tttaattcaa aagtatatag ggctatctat ataccctcat ttattatatc agcagggtta
    10921 tttctatcag taattattca aaaattttta aaagaatatc aaaaaaggcg aatccttgtt
    10981 tttcttaatc ctgatcttga tccctatgga tcaggataca atgttataca atctaaaatt
    11041 gctattggat caggtggcat tattggtaaa ggttttttaa aaggatctca gtcgcaatta
    11101 ggctttttac cagaaaaatc tactgatttt atcttctcag taatttctga agaatggggt
    11161 tttattggag cggttattgt acttttatta tttgggtata tactttataa aggatgtatg
    11221 attgctttaa aaacttcgga taaatttggg atgttacttg ccggaggtat aacatgtatg
    11281 atattttttc atgtatttat taatattggt atggctttag gtataatgcc agtaacaggt
    11341 ttacctttaa cctttctttc atatggtgga tcaaacttga ttatgtcatt gttgagtatt
    11401 ggtattctta ttaatattga ttcacgcaca agataatatt ttattatata aaaaataaaa
    11461 taaagaatgc ttgcaaataa tttgctttca tgtatactat aatgggtatg tttttttgat
    11521 ttcgattata taggaggaca aatgttaatt gtccttttta tagtattgca aatttctttt
    11581 tctttccata tatattcgct tgtattatat gtcttacgaa gagaaaataa atatttaaaa
    11641 ggcttcatta atacaactat cagcaatata ttgttagccg gtggaataac tgctttagct
    11701 ataatacatc cacaaaatgt caaagaggtt gactttaagc ttctgctgtg gtttatgaca
    11761 ggatttataa tgctcattat gttatttatt aaaataagta ttgcacgagc gatttttata
    11821 cgtacaaaag atccgcaaaa ttatcatttt aattattttg gtaaaaaagt attacattct
    11881 actgtggtaa gatttgaaga aattttaata ttctttttta caatgccttt ctttttattt
    11941 tgcggagcct attttattgc aaggctgttt aacctgttat tatataaaca tttataaaaa
    12001 tattattaaa ataatttata tattgttcat gttatataaa aatatgctgg attttttatt
    12061 gctattttca tattattgta tacagtatta taatatctac aatcaatgac gggaggtgtt
    12121 ttaatgggtt taattaccga taaaatacgt gaaaatgctg agaaatgtaa atcctgtgca
    12181 tttctaaaaa taaggaatgc agaaattgat aaaaatagcg ttctttatga tgaagctatg
    12241 atgacattag aaggggttta tagggctagt tgtacgatgt gcccttttga gaaagatttt
    12301 gagaagattt atggcatgaa accctatgaa tattttccaa tgcggatagc aaaaaaggaa
    12361 gcataataag tttaattaca caatgagtaa aaagaaatgt gtagtgttgc tcagtggcgg
    12421 attagattct gccactgttg ctgtatttgc aaaaagcagg ggatttgata tttatgccct
    12481 tacctttgaa tatgggcaac gtcacgagat tgaaattctt tatgcaaaaa agatagccca
    12541 ctatctgaat attaatgctc atgatattgt gcatttaccg gatgacatat ttaaaatatc
    12601 atctctggtg ctatcatctg gcaaggaaat accaatcacc cgtggaagtg ctatcccatc
    12661 aacctatgtc ccggcacgaa atattatatt tctttcgatt gcattagctt atgcagaaac
    12721 agtagaggct actcatatct ttattggtgt gaatgcggta gattatagtg gttatccaga
    12781 ttgcagacct caatttatag aagcattcca gaaaatggta atggtgggta caaagagtgg
    12841 ggttgaggga aatccaatta ttattgaagc accacttatc aatatgacta aagcagaaat
    12901 tattgcatta ggtacatcat taggtgttga ttattctctg acatcaagtt gctataatcc
    12961 atcacctgaa gggaaaccat gcggtatatg cgatagttgt atgctaagaa aaaagggatt
    13021 tgaacaggca gggtatgaag atcctgccat acaacggtaa aactgcaaaa aatatgaaac
    13081 agagttatat taatatactt ttaaatactt taatgtttga tgtactattt cctgcatttt
    13141 gttctaattg tgggaagatg atatcaaata tcaataatgg tttatgcaac aattgttatg
    13201 ataatcttaa tttgagtgtt tataattaca gcagtcaata tttttttaat gttgaagaaa
    13261 agattcatgg taaacgttac tggtatattg atgatatatt attagttttt aaatatagaa
    13321 acgaagtatc aaaactgata tatgatatga aatttaaaaa caataaatct attgctttat
    13381 tgttttcaaa atttctgtta caggggatca attctttagc atgggatttt gatcttataa
    13441 catatattcc ggtttctaaa aaaaggttat gggaacgagg gtttaatcaa tctgaactta
    13501 tggcacggca tattggtaaa atgctacata aagatgtttt caatttaatg acaattaaaa
    13561 atgaaaacaa tacacaaaag aaattaagcc aaaatgatag gtttatcagt acatatcaaa
    13621 aatttgatat tttaagtaat tgcactaata ttgtaaacaa atcagtttta atagttgatg
    13681 atgtttatac cactggtgca actattaatg aatgcgcgcg tattttaaag atgaatagtg
    13741 ctaaaagtgt ttatgcatct atactggcat atgcaatgtt gaaagatagg gatgtttaat
    13801 gttatttata ataatacatt taacagtgtt tgaagtgact actactgtaa ttattttata
    13861 taaatatctt ggaaagtttt gaacactaca gcaattgtaa ttgagcatta gagtgactaa
    13921 aataatggtt gtaattacat tcgcgatagg ttaaaacaaa tagtatatct acttcaggat
    13981 taacaaagaa taagaggtga aatatgaaaa ttaaaaggga tgataatctg gctattctta
    14041 ctttagatgg aagaattgat caggagatgt cagaagatct tgaaaatgca ttacagaaat
    14101 taattgatga tggttgtaat aatatttgta tggatatgac tgatgttaag catatatgta
    14161 gttcggcatt aggtgtgata gtagctttta aaagaaagat aaaaaatgaa ggtgatataa
    14221 aattagtaat aactaatgaa aatcttctta aactttttca aaccaccatg cttgataaag
    14281 tatttgaaat atttgaatca caacgtgaat gtttaaatgc ttttgattaa ttgttgataa
    14341 aagagatata tatgacgcca cgaaaagaca tacacgacat actaaagcct attgaacatt
    14401 atttaatggc tgtggatgaa acagtaaaaa gcaaattata tacaggcatt gatttgctgg
    14461 atgatagcag catgcatacg ttcaaaaaga gcggtaagaa gataagggct tctatgatta
    14521 ttttatccgg tggattaaac aatactatac ctgacgaaat tattgacata gcctgtgctg
    14581 ctgaaattat tcatgctgca tcgttagttc atgatgatat tattgataat gccgatttac
    14641 gaagaggctt gccaacagtt tcaaggaaat atggtcccaa agttgcagtg cttgctggtg
    14701 attacatgta cacaaaagcg ctggaaatag ctgttggcaa caatagaaca gatctcttcc
    14761 cgataatggt tgatgcaact gttgaaatgg ttaaagggga gctatatcaa attcagtatt
    14821 ctgatattga taatataaca attgataatt actatcgcat aattgagatg aaaactgcac
    14881 gttttatggc agcatgcgca aaattaggtg ccgctgttgc caaaatgaat acacaacaat
    14941 ctgatgtatt atatgaatgc ggattgcagt tgggatatgc ttttcagata accgatgatg
    15001 ctatggatta tctcaatgcc gcggatatta ccggtaaaga tgctggcaat gattttatta
    15061 atggcaagat taccttgcct ttgttgcacc ttttagaggt agcagataat aaagagcgtg
    15121 cactgttaaa aaattatgca aaaacccctg ataacgataa ttggaattat gtagtatcaa
    15181 aggcaaaaga atataatgtg gttgattatt gtataagtat agcatcagat tatgtcaaaa
    15241 aagctattga aattatctct atgtttccag acaaccaata taaaaatatt ttaaccgaat
    15301 tagcattgtt ctttgttgac agaaaatatt gatataggta taatttatta ttaaacttag
    15361 ataaaatatt gtaaaggaaa tagtatatga taacaaaaga aaaagaggaa gttttacgat
    15421 tatatgatgc tggattggag gcttataaaa agcgtaaatg ggatgaggct attgattact
    15481 ttgataaggc acttaaagtt gatcccaatg atggtccttc taaagtatat cttgaacgag
    15541 caagacaata taaacaaaca cctccctctg atgattggga tggcgtcttt ataatgacta
    15601 ccaaatagat tgttttttaa aatttggctt gtattaaaaa aatcatgtat caacattgct
    15661 gttataataa tatataataa atatttgttt aatagaagaa ggtatataca atggctaaaa
    15721 aggttataca ggttaataag aatccagtat atgaaattgg aatgatagca actgtttttg
    15781 gtaaagatac agagttttat ggtgatctta cctttgaaaa atctcttcaa attaatggtt
    15841 attttgaagg tgaaatatcc tcaggtgatt ttctggtgat tggtgaaaag gctaccgtta
    15901 aagccaatat aaaagccagt acggttatta tcagaggaac tgtttatggt aatattgaag
    15961 ctaaagataa gattgaaata caaactgaag gtaaactttt tggaaatatc cgtacatcaa
    16021 aacttatcat tgcagatggc gtagtatttg aaggaaaatg tgaaatgatt aaacagccca
    16081 tagcagttca aaaatcaaaa caagtccaca aagaaagtac tctggttgaa tagatagtat
    16141 aatctgttgg caatatttat ataattacaa ttaaatgaaa gaatttgtta aaaaaattga
    16201 caattgtttg tatgaaattc ctgaagatta taaaaaagga atgcgtgtgc cagcacgcat
    16261 ttatgcatcg tatgagcttt tgcatcatat ggatgatgca gtatttgaac agcttatcaa
    16321 tgttgccatg ctcccgggta tacaaaagta tgcattatgt atgcctgatg gacactctgg
    16381 atatggattt cctataggtg gtgttgcagc ggttgatagt gatgatggtg ttatttcgcc
    16441 cggtgggata gggtttgata ttaactgcgg tgtacgtttt gttataacct cagtgagcat
    16501 tgaagagttt gcacctaagc tgaaccagat aatggatatt ttattttcac gcattcccag
    16561 tggtattggt gggaagggta ttgtttcatt atccgataac caatttgatg aagcaatgat
    16621 aaaaggagcc agatgggcaa tagagaatgg ttatggtgta aaggaggatt tattattttg
    16681 tgaggaaaac ggatgtattg gcaatgccaa tccagatgtt gtttccggga gagcacgtga
    16741 acgtggaagg gagcaggtgg gttctttggg ttcgggaaat cattatcttg aatttcaggt
    16801 tattcgcaat gaagatgttt tacaaacaac tatcgccaaa aaaataggca ttattgggaa
    16861 taatcaggtc agttgcatga tacattgtgg aagcagaggt tttggtcatc aaatagcaac
    16921 agattattta gataagttta ttggggtgat gaagcaaaag taccatatag atattcctga
    16981 tagggaactg gcgtgcgcac cgtttcattc taaagaagga caggcatatt ttggggctat
    17041 gaattgtgcg ataaatattg catttttaaa tcgccagctt atatatcatg ttgtacgtga
    17101 taccctatgc caggttttta aaaaatcagc agaagaatta ggcatagcat tgatctatga
    17161 tgtttgccat aacacagcaa aattagagca ataccagatt aatggtaaca ataaaaagtt
    17221 actggtacac aggaaaggtg caacccgtgc ttttacgaaa ggtatgaaag ggatacctga
    17281 tacctataaa gaaattggac agcctgttct tgtaggtggg agcatgcaaa cagcttcata
    17341 tatattaatt ggtggagaca gggcttccga tgcattttac agcactgttc atggcagtgg
    17401 tagaatcatg tcacgacagc aagcaaaaaa gcaatttaag ggtagggagc ttgcagatag
    17461 catggcgaaa agcggtattg ccattaaaac acgttcattg agtggtttag ctgaagaagc
    17521 tggagcggcg tataaaaaca ttgatgatgt tattgacact attacagcag cagggttaag
    17581 tattcctgta gcacgaatga taccagtagg ctccatcaaa gggtagttgt gaacatgatg
    17641 gaaagtgaag aaggttttga atttgttgaa aatgcaactt tagcagatat agcaatttat
    17701 tgtaaggctt ttaccttaga aaaactttat gctttcaatg ctgaagcttt tcttcatata
    17761 tgtctggaaa atccaaatga cttaaatgct aagattgatt acaggttttc tttacaacat
    17821 caatctcttg acctgttgct ggtaaaattt ttaaatgaat taatttttat taaggataca
    17881 cagggtatag tatgtaaacc agtacatgtt gaaatagagt atgggcagga aggtagtatg
    17941 tgtacagcgc aattgatagg tgattatata attaattacc ggattaatgt agatcctaaa
    18001 gctgcaacat tacataaagc tcgttgtgaa aaaacaaact taatatggga atcatttata
    18061 attattgatg tatgataaga aaggaaggtt acacaatggc aaaaataata gatgggaaag
    18121 caatagctaa tgaaataaaa caggagataa aaaaggatgt tgcaaaactg gaacaagaat
    18181 atgggaaaaa accaacactg gctgtgattt tagttgggaa tgatagtgca tctgaagtat
    18241 acgtgcgcat gaaaaagaaa gcatctgaag aacttggcat aaactctctt cagattacat
    18301 tagatgaaag aacaacacaa tctgaattgc taaaattgat acatgagctg aatagtgata
    18361 ccaatgtcaa tggtattctt gtgcaactgc cattaccatc gcatattgat gaaaatataa
    18421 ttattaatac catcgatcca ttaaaagata tagatggttt ccatcccatt aatctgggta
    18481 aacttgttat aggtagtgac gactgctttg tttcatgtac tccgtatggt tgtcaggagt
    18541 taattgtacg caatgtgccg gatttaaaag gaaaacacct tgttattgtt ggtagaagca
    18601 atattgttgg taaaccatta gccaatatca tgttacaaaa aaatggaaga gccaattgca
    18661 tagttactat ttgtcatact gctacatcgg atattggata ttatacacgt caggcagata
    18721 ttcttgttgt tgctgctggt aaagcaaata tgataacaaa ggatatggtt aaaccaggtg
    18781 ctgttgtcat agatgtgggg ataaacagaa tagatgatcc ccgtaatcca ggtaaaacaa
    18841 aactggtagg cgatgttaaa tttgatgagg taagtgagat agcttctgca attacccctg
    18901 tcccgggagg tgtggggcca atgacgatag ttatgttgat gaaaaataca gtgaaagctt
    18961 ttcgattgca aaataagata taacaatgat gtggcaacat tgcatttcag caacaataaa
    19021 aaagggtgta atggaacacc cttttttatt gtattaatat ttactcgaat actgttttaa
    19081 tgagcttctg tgtgtttttc agggaatagc ggtatatact ttgtaaacca catatagatg
    19141 agtatcatgc atgcaattga tccaaagaat attgaccatt cctgaatggt tggattataa
    19201 gtaaagaaac cttcccatga aaagtttggt atggaaaatc catggagtaa tacatcaact
    19261 tttaacatca ctattgcaaa aactccactt atcaaacctg caataaaata ttgctcattt
    19321 tcacgaaatt ttttaacatt aagaataact atcgcagtta gcgaagctaa aagttctaga
    19381 gtaacaaccc accaaccata atagcctccc atcatctcga taaagcttct gtcaaaaagt
    19441 ggtgcaaaat gggtccataa tgagtatata tcatatattc tgaacaaaaa gtacgcaatg
    19501 aatgtaactc ctgcaacctt tgctaatgtc atataggcat ctctgggtac taattctttt
    19561 tttgcaaccc agcttgctat tttaggaact aacatcatga ggcagggtcc acccgctatc
    19621 gcagcaacta tcgccagata aaaatagtga tgtctgaacc agtatggttt tgcatataac
    19681 actccccaca tcccgccacc taaggatccc tgatggaaga aagaaagaaa tgttccaata
    19741 gcggcaaaaa tccacataag tttatgtaaa taatgagcaa aatagtgtac tgcggggtat
    19801 ctatcaaggt atttattgct cagtataatt gggaaaagct caatacaaag aacaataaaa
    19861 taaaacgtta aacaccatac tacctcggtt aacattgatt gaggcatggc atgaggaccc
    19921 caattgggat aggcataacc aaaccatgct cttagtggct gaccgatatc aaataccaat
    19981 aatatgaaag taaaaagata acacataaaa cctatcagca cagttgagtt aattaaaggc
    20041 tgtaatttat caactctaaa tatgtatagc aggaagcccg taaaaaaagc tccgccaccc
    20101 agtgctacaa aacctaaatc accgataatc cacaatccca tagcaaatcg attattcata
    20161 tcggtataac ctaatcccca gaataatatc tggataccaa aaatgacaaa taacgctacc
    20221 agggcaaaca gcacatataa tgatatctgt atattcctgg aatagctgtt ccattcatct
    20281 tttaagaaat tgattacttt gctcacggtt aactctcctt ttgccttttt caggagtttt
    20341 taaatccaga tattttatca cgcagccatt gctggcttgt gagataatat acttttggat
    20401 ttggataatc tttaattttc aatcgttttt catattcaga aggatctttt gttcgtaatt
    20461 catctataga atgaacaaga cgaactgctc tgggattctt ctgtacagca gcttcatata
    20521 ctggtgatga gggatcatta aggtcgccaa agattattgc atttgttgga catgcagatg
    20581 cacatgctgg tgtataggta acagcgttga tgtcagtaac accgttagca atagctctat
    20641 cacgctcacg tttccatata tgtgaacaga aagtacattt caccatggtt cctcgtgatg
    20701 caatagaaac atcaggattt aaggactttt tgaagctacc ttcataacgc ggtttccacc
    20761 agtgaaagac gcgggcttca tacggacatg caacctgaca gtatctgcat cctatgcaac
    20821 gtgaccatat ctggctaaca acaccatcct cgcctacatc agttgccacg accggacaaa
    20881 cgctaacaca tggtgggttc ggatgattgg ggtcattacc tgaacactgc tgacacatct
    20941 tgggtaaata tgcaacctta acatcaggat attgtgcttt aggatcttta tcgttggtta
    21001 cttctactaa atcaaggaag gtaacccgtt taggtatatc agaatcatct ttaaaaatgg
    21061 gtagattatt ttcctgggaa caggcaatat tacatgcccc acatccagta catttatcta
    21121 aatcaattac cattccccat cttcttgtca atttggccat aattattcac ctcttaactc
    21181 aattttactc gtgttaacca ccaatcggca taaccactgg ccgggtcgat agcatctgac
    21241 atgataattt tagggttaac acctttttca ctggcataat cagtaaattc ctcatgacca
    21301 aaaccgattg gaattgcaac agtatcgtaa gcaattcgtt ttgtaaggtg gactcgtact
    21361 gtgtcaagct caccacggga agattctata tcgattttgg aaccttcgga aattccttct
    21421 ttagaagcag ttgctggatt catcaacacc cagcttgtat cacctttcag aatagtacca
    21481 ggtaaacctt ttaatacata cggcagtgat aatccactac catcgctaac agttggtatt
    21541 tcaaaaggta tcattgctat tgagtatgaa cccgatgcac tgagtatttt ttcagtttct
    21601 tccaattttg taataatcaa atctaccggt atatctacat tagcaatatt ttttgtttga
    21661 tatttaacgg tttgaaccgt ttctaagtaa ttcttccatg agaattgcga tgatattttt
    21721 gcaatatttt ttgctattgc aagcagtgaa tccccagcat gtttagcttc tgatgtaggt
    21781 gtcacaactg cattgcctgc aacaggcaac tccaacgatg ttaatgttgg aaaaacataa
    21841 tctgcataga gagctgtatc attgagcaga gatgtacaaa gaacaaccgt tgaagctttt
    21901 ttaagcttat cagatagtcg ttgaccatat acacttgagt acacaggatt ggcattattg
    21961 attagtgcta tatcacagac actatcgctt gctatatatt catccaatcc ttttgctttt
    22021 tttggtataa caggtttagc tggagttgta aaacttacca gtttaacact gcttcccatg
    22081 cttccaatca tgctgttgat ggtctgtatt attgcaaact cggcaacgga agaggaatta
    22141 acttttcctc ctctaccagc tacagcaact ggtctttgag cactgataag agcctttgct
    22201 actgattcaa ttgtatctaa tgaaactccg gttagctctg aaaccttctc aggtgtatac
    22261 ttgttgatta acaattcagt gaatcttcca aaattggtcg cacctgatcg tttaccatat
    22321 ttcatcaggt aatgagcaac acccatagca aatagcggtt cgctgcctgg ttttattgcc
    22381 acccactggt cagccaatga agcagttcta gttgcgattg agtctatttg tataaattta
    22441 ataccttttt tcttccataa tgacaataca ttatgcattc ttcctgggtt accccatcct
    22501 tcaaaaagac gagcaccaaa actaacaacg caatcagcat tttcaaaatc ataatcaata
    22561 gcgccatcag ttttaaaggc tatctctgaa catgcattgc tgagtgccca gtaacctgat
    22621 tcctgaaata cgtatgggct accggaaaat aacgctatcc tctcggtcaa cattgaggtt
    22681 gcatcaggat atgcaggatt aatggctata atctgttcct gtttattatt attgatagca
    22741 tcattgatct ttttggaaat atcagcaatt gcttcatccc atgataccgg tgcaaaatca
    22801 attttacctt ttttaccggt acgcttcaat ggagtggtaa ctcgctgggg atgataaaga
    22861 acctgtaaca aaagctgccc tacagcacaa ccctggtttg aagtttcaat tttgactgct
    22921 ctgttgccaa ttttgcgtac ctgaatggta catttatcag ggcacctgtc acatatactt
    22981 gctaagtatt tttcttcacc ctttggtggg cgatactgat cctgtgtcca ttcaactaac
    23041 cactgtaacc ccatccatgg gccaggtgat atcagggtac ctacaccggt accaacaacc
    23101 gcccccccaa tcattgttaa gaagtctttt cgttcaagtt tcatggatgt aatagctcct
    23161 tacttgtttt tcaattcata aaataagaat gattaatcat gacatactgt acacgagttg
    23221 cttacatttg taactgtgtg acagtccata catacaccca ttgccatttt acccttgatc
    23281 ttagcagttg ttttagaagc tcctttgtcg ccatgacacg aaatacactg aaccttttgc
    23341 tcattttttg tcacagctat atgactgaaa tatactaaat cgggctgctg cgcaaatgtt
    23401 tcccatggct tgtcagtgtc attaaatccc gcaaagaata ctgcattatt aactttgtta
    23461 ggatcatgac attgcttaca tactgctacc gtagggatac cattaaatct tccattcgca
    23521 tcatagctgt ggcatagttc acagtcagca ccgtactgag ttgtgtgagt ttgatgattg
    23581 aatggaagga gcttttttgc ctgtacattc attgcataat cacaggcctg ctgaaataca
    23641 ataaatgcga tgtatgtaaa aataataaaa ggtaagaaaa aaagcagaaa agaacgtgtc
    23701 cctttcacag acgtgctcct taatctttat agtggtatag atactttaaa aataaaaata
    23761 tttaaaaaaa gtggattgta tttaatccac agaataagta catatgcgaa ttcttgtata
    23821 tacaatctta atgtcaacaa aattataaaa aaaaatggaa gggaaagcag tgtaaatcca
    23881 ttattttaaa ttactgctta tttaacgtta acaatggttt ttgctatgct tacagcatca
    23941 acggcatctt tgccataata ggcgccaatg ctttcagcat attcttttgt tactacagcc
    24001 ccaccaacca taaccttgca gggtaattgc tggtgcttga tcatggtggc aatctgttcc
    24061 atgttgggca tggtggtggt cagcagtgag cttaaacaaa caatatccgg tttatgttca
    24121 ataatggagg caatgacagc ttcattggga acatcttttc ccagatcgat gatatcaaat
    24181 ccatgatttt ccagcatcat cgctacaata tttttgccaa tgtcgtgaat atcgcccctg
    24241 acagtgcata tgagcacctt tcccagcagg gattctttat gttgctgcat cagaggtttc
    24301 agtacagtaa aacccttttt cattgtatca gcagaggcta tcatttgcgg taaaaaatat
    24361 tcaccgctac tgtacaattg ccccactttt tccagagcag caatgagggc attgctcatt
    24421 atgatttccg gctcatacat ggttaatgcc tgatgaattg catgttcaat atcatcaatg
    24481 ttgccctcaa caaccagtga aaagattcta ttcagtatat cactagattt ttcttttact
    24541 ggggattgta ttctggattg ctgctgtgat gttccaaaat gggcaatata gtgcacggca
    24601 tttttgtctc tgccttcaag aaaatcaagt gccattgttt cttcagcagt aaaaggtttt
    24661 tcagtattgc tgtcgatagc agaaggattg acaatggctg ctgtaagccc tttttgaatt
    24721 gctaacttta aaaatgcatt gtttatgtac tttcgctgtg gaagtccaaa cgaaatattg
    24781 ctaagtccta tgcttgtttt aataccatgt tttgaaaaat attctattac tttcaatgtt
    24841 tccattgcag cgccgctttc agcgctttcg gcaagtataa gcggatcaat aaatatgcgt
    24901 gatagttcta tgccattatc ctgcaacagg gtaataagcc tttgcccgat ggcaattcgt
    24961 ttttttgctt ctttgtgaat gccgctttca tcaagacaca ttgcaatgac aaaagcgcca
    25021 gattgttgca ataaaggaag catactgtta aaactttcct cttttgcgct gatagaattt
    25081 aaaattggga tacccggata gtgccatagc gcagcttgaa tgacagcatg attgtccgaa
    25141 tcgatcatca atggtatggt aactgtttta gataggatag taacagcctt cgtcatcatg
    25201 atgctttcgt cgatgccggg tacacctaca ttgatgtcca gaatatgggc tccttcatcc
    25261 tgttgctttt ttgattcctc acgcaaaaaa ctaaatttgt tttcttttaa ttcctgtgca
    25321 aactgtttgc gcgctgtagg atttaacctt tcgccaatga taattaatcc ctgatgttgc
    25381 tgcacatcaa tatatccgta gcgcgatgta atgtaatgaa atgtttttga ccgtggaata
    25441 tctattctaa tctctttcac agcatttgct aaagcgttga tatgagcagg agtggttccg
    25501 cagcagcccc caataatgga aaatcccata gtggcaaatt gagttgatag cattgcaaat
    25561 tgttgtggcg atagtgaata ggtagccttt ccattgacaa gcgtgggcag acctgcattt
    25621 gcccatacag acagtggtac gccaagttgt ttaagctgtg cgatagctcc tgtaaaaagt
    25681 tgcaccagac catcaggtcc catactgcaa ttggcaccca ccacatcggc acccatctgt
    25741 gaaagagtgg caaacgctgt aacgatatcc gttcccgtaa ctgttttgcc gttatcttca
    25801 aatgtcatgc tgcatataat gggaatattt gtgacagctt ttgcagcaag cagagccagc
    25861 cgtgcttcct gcaagtcggt catggtttca atggcaatac aatccactcc gccatctacc
    25921 aaccctttga tctgcttctt aaatgcatta tagac
//
LOCUS       DAOOJH010000027        25887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_1957_length_25887_cov_9.343644, whole genome shotgun sequence.
ACCESSION   DAOOJH010000027 DAOOJH010000000
VERSION     DAOOJH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25887
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_25887_cov_9.343644"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..950
                     /gene="radC"
                     /locus_tag="PLJ75_05465"
     CDS             243..950
                     /gene="radC"
                     /locus_tag="PLJ75_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPD04699.1"
                     /translation="MRLIFLLQEDAMQSKNDLPIQRCIKGEPLEALSDVELLAVLIGS
                     GVKGKNVMECAFYVIEQFGGMNGIYHAGIRELAHTDGIGFVKAIRIKAAFELGRRLIV
                     PKNYEGSIDSPRMVWEFIVGQIACNKQEEFWALILDNKNRLLKKTRVSIGTISESIVH
                     PREVFRDAIREAASSIIIAHNHPSGLLKPSVNDIEITRRVAQVGAIVGIQLLDHVIVT
                     DNDYLSLRETDEKLFLA"
     gene            1187..2056
                     /locus_tag="PLJ75_05470"
     CDS             1187..2056
                     /locus_tag="PLJ75_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HPD04700.1"
                     /translation="MKYKKPGTIVLIAITVIFIHATLVSDTRHGTWVIYNVPASPQSE
                     KTIPVKIYFPKSYSDSSRVIIAFHEYDGSMNSWQNNTSIAYYANQYNMVIVCPHMPRS
                     VYESYYFDQTTVKWNEMPALLWIGQLLLPYLNNTVGLNITKQKLGVCGFSIGARGALR
                     VGEVYHDFVGAIACLSGYYDMLSHTKNHMFTAVYGKYNDFQQRWAQTDNAIDEAKNLG
                     DVAVFIAHGNKDSRVPMEQAFMLALKLKQLQKEYKSGYNFTFVEKKYKMHDWNYCQSV
                     LPDMMAFFNENLK"
     gene            2065..2580
                     /locus_tag="PLJ75_05475"
     CDS             2065..2580
                     /locus_tag="PLJ75_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04701.1"
                     /translation="MKNFRAIVPFIIVLVALCCSKHGVRSGAMLCTMHNAHNFYSKAD
                     FARCMGIDENALEDYFIFSQSAKAFSDTASTESMLKSTVCDTIVDEVMLRIQTMFTNK
                     QFARWHFTEMSLPVQFRTFIQQGSIPEMMVVGLVKKEDLSARAIIYYLPLEYKMDIIG
                     REKENGKMPDW"
     assembly_gap    2635..2734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2791..3558
                     /locus_tag="PLJ75_05480"
     CDS             2791..3558
                     /locus_tag="PLJ75_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPD04702.1"
                     /translation="MLTLGMDIGSITAKAVVLNGGTIVGSKVIFTGFNAAKAGEKVVQ
                     ELLAELGLTLEQIERIVATGYGRKSVGFAHATVTEITCHARGAHFLDNSVRSIIDIGG
                     QDSKVIVIDETGRVKDFAMNDKCAAGTGRFLEVMARALEVDLDEFGELSLKADNPSKI
                     SSLCTVFAESEVISLIAKGESRDNIIAGIHQSIASRVMAMASRVGIASPVMMTGGVAK
                     NIGVVNAIEKLLNSPVKVSKDAQVIGALGAAILAAEN"
     gene            complement(3559..4056)
                     /locus_tag="PLJ75_05485"
     CDS             complement(3559..4056)
                     /locus_tag="PLJ75_05485"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HPD04703.1"
                     /translation="MTYLIDGYNLIYKFPHLEELMLQDNLIEARRELLDLLKMFAKLT
                     RKRIRIVFDGQKTSDIPISSEKVYTIDVYYSLHYSADFLIKEFIRKDLQPRNTTVVTS
                     DKDIIEYVSRFKTKTMKSEDFALFVNQTIEDFTLAQTPEKEENPNLSQKEIEYWERLF
                     TSKKK"
     gene            4279..5541
                     /locus_tag="PLJ75_05490"
     CDS             4279..5541
                     /locus_tag="PLJ75_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04704.1"
                     /translation="MTDSNNEQKYNVAYLERQLFNLSKLVEINRIINSTLDMAKLFTI
                     IMEIIKEIMETEASTLFLFDEKTNELVFKVALGEAGDKLQEKYRVAMGQGIAGWVAKE
                     RKGVYVNDVYLDERFDPNFDKQTGFTTNSIICVPLLYKGKLLGVIQAINPINRPDFTD
                     EDMNLFRAFADQAVLAVQNAIFFERAIEEARMRNELSAAHEIQHSLHPSIANDYVFCE
                     IAARYLPAREVGGEFYEIKPYDDAVAIAVCDVHDKGVPGALNAALVNGLLKGLMNVYG
                     RVVAPIFLNTLQVVASHHINNVSLFLGMIDNKTKTLTFMNTGEAYPVIIRDSIAHYLR
                     FSHSLHNNKKIVVKLKHGDMVVIVTDGVVNCKNRNARLFGLKQVMDTCSQFSTPESCI
                     AGLLDTVKNFMQGLTQREDISVIAVKVK"
     gene            5558..5773
                     /locus_tag="PLJ75_05495"
     CDS             5558..5773
                     /locus_tag="PLJ75_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04705.1"
                     /translation="MEYVSVILCRNCGSRYVEVNEWTQDKKAVFHCRTCGKKEIVEWF
                     TLGRCQVTPSELQKTRDTKAKPGKYER"
     gene            complement(5774..6586)
                     /locus_tag="PLJ75_05500"
     CDS             complement(5774..6586)
                     /locus_tag="PLJ75_05500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HPD04706.1"
                     /translation="MIKDAIYYDSYKEDRDLILEYNQTSLADVNLVNILGIGIDNCTR
                     QQAVVKVLRMIEEGGVHHVMALNPYKLHRLITNNDLNLIASKADLKIAVWAGLPWAAS
                     FLGKPLKERIHFVSFLMDIIRIAEIKEITIFMVGAKPEIIEQAYANIKKSFPMIRIVG
                     RHGGYFNKERASNVIEAMRKSEAQIILVGLGFPKEDKWIYQIKNQFKNTVFIGIGGSI
                     DIISGQKHKAPAFFMEKGLDWFYRIITRPWRYLRLLRLLLFYIQVVWQKIRQ"
     gene            complement(7126..8274)
                     /gene="alr"
                     /locus_tag="PLJ75_05505"
     CDS             complement(7126..8274)
                     /gene="alr"
                     /locus_tag="PLJ75_05505"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPD04707.1"
                     /translation="MILTNPAYCNRPTRAEISIPNLLNNFAIVRSLISPQVKVMAMVK
                     ANAYGHGIVRISQELIKAGVNYLGVAYLEEALFLRQHGIHTPILVCGAINSEQIPEFI
                     INDVAITSSSIDKSKAIQTAAKALGKKALVHLKIDTGMERIGVHWYNAQKFIEETLQL
                     PDIQVVGIFSHLAKAESDSHFTQEQITRFESIVNYMDRQSILPPYVHLANSAAIINNP
                     ESHFTMVRPGIMLYGYNPNGYNPSIEFNRKKLLPVMTLKTKVSYFKVVPANTGISYNH
                     LYKTTTQTRVVTLPVGYGDGYSRLLSNQGEVIIRGKRYPVVGAICMDQLMVDIGYDGT
                     AYNGDDVLLFGQMDGYSIPLESLCEKIGTITYEVLTNISPRVPRIYIQ"
     gene            complement(8400..9119)
                     /locus_tag="PLJ75_05510"
     CDS             complement(8400..9119)
                     /locus_tag="PLJ75_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04708.1"
                     /translation="MTQSKIITFDTIAFPVDNSLLKLLHQKTDCQELSHSLHKGSDAY
                     INTINTFSGDSLSNNQLLSALPDSIYQLYVQAYHIAKPEAAIGKANAINSTIAIPDAI
                     VTSDRSYAFIFTLGIRYENWYISLPGLTHQYHASIIGMWLCNQYVQELLKKLFGTGGY
                     VWYPGSSKELPVTLNHAIYAMLKTQAQNIGIALNRHCMFQPLHTVAGFALPYGNSHSV
                     CSSCNLQQCQFRNILTELDNC"
     gene            complement(9135..9207)
                     /locus_tag="PLJ75_05515"
     tRNA            complement(9135..9207)
                     /locus_tag="PLJ75_05515"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9172..9174),aa:Trp,seq:cca)
     gene            complement(9254..10696)
                     /gene="purF"
                     /locus_tag="PLJ75_05520"
     CDS             complement(9254..10696)
                     /gene="purF"
                     /locus_tag="PLJ75_05520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPD04709.1"
                     /translation="MKTEYEYCDCKPREECGVFGIYNNPRAANLTYLGLYNLQHRGQE
                     SSGIASSDGEHIYRYAGMGKVVDVFTKEHINNLKGTIAIGHNRYSTTGSSFLRNAQPL
                     RVHSVLGPIVLAHNGNLVNYAILRRALEKEGAIFQTSIDSEVIVHLMARSGLTNFLDA
                     LIYALKAIKGAYSLLVMNNNTIYAIRDPYGFRPLVLGKLGNSMVVASETCAFDIIEAE
                     YIREIEPGEMVEISPNGMKSYFPFGSVKNSLCVFEYIYFSRPDSIIFGQSVHEMRLRM
                     GAVLAKQSPVKADIVVPIPDSSVCAALGYSRASGIPYEMGMIRSHYIGRTFIEPEQRI
                     RDFGVKIKFNIVRSAIEGKRIVVVDDSIMRGTTMRKIIKMFRNGGAKEIHVRISAPPT
                     RFPCFYGIDIPTRSELIASSHAIDEIQKYLRVDSLAYMTVETMLASLGVHHGKFCTAC
                     FDGNYPIEFQESEDYQKCLFDDIVACGEYY"
     assembly_gap    11096..11195
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11196..12052
                     /locus_tag="PLJ75_05525"
     CDS             <11196..12052
                     /locus_tag="PLJ75_05525"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD04710.1"
                     /translation="VIVFDNFPTGKEENLSFIKQYPTARIKVIHGDVADWYALHEAVK
                     GCDAVFHEAAVASVQQSIENPPATFESNAKGTLHVLEACRQNGVKKVIFACSAAIFGD
                     HPEIPKKENSPVFPKSPYGADKYISEVYLRLYNELYGLTTVSLRYFNVFGPGQDPSSP
                     YSGVISIFTKRIAMGKDITVYGDGTQYRDFIFVKDVVQVNMLALEKLDKGFHYYNVGY
                     GKATDINSLITILQNIYNKTIKVHYVEARPGDIKESISDPAKVIRELGFSPQYSVEEG
                     LTLMAGVM"
     gene            complement(12053..>14231)
                     /locus_tag="PLJ75_05530"
     CDS             complement(12053..>14231)
                     /locus_tag="PLJ75_05530"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04711.1"
                     /translation="LYKNTYKRAMYMITLSLIGSFLASCMLIFTIVYGLYKNFQDRVM
                     RYYSYFGFCAFGILFTMFLTYAFPDKLDLTLINKITQASTVLTFSALFVLSLIFPKTE
                     KQVPFFIIVLILIPAYIFAYIAVFTDLNITAAYFKDGELVREFKPFYTIYSVVSFVYI
                     LLGVINFIRKYITTKIDLYRKQMRFVFIGTGIAMTFAAVFSIIMPRFFNFVDLYVLGP
                     SLAAFIGVSSLFYAVISYQLMDIRTAIHKTTMYAIISAVIFLPIFALLQIYDINIYGL
                     KNIPVFVIALVIVVVFMILSKYVQPVIDKAFKRKHYALQEYLDTFVREISNLRDIDEL
                     LKRTVSTIHNTFFLKTTYILMYDNESRSYQINYSMGMAPVSPSIPRLSPIVRWFNRNQ
                     TIVTIDNVYMDEASFKDVRDEFIQYFTVNNIVIMIPIYHRRVLVGALCLGEKDTFASY
                     KPDEIELLKFLSSETNINISHAITFQEAKKEQLLQRTISVSSDLLSKAVPKALPNITG
                     IKFGAFYIPRQGGGIDYFDFIRPGLQGIGTIATDIAGIGIQSALYSVLMRSAFHASLS
                     DAHSSYNIIQTLNKVIYNYSQGKGILITAYYYFIDARNMRLIYTNAGFPPMELYRVEK
                     SAFSSLDTEGIPLGYDPTFNYGMGRTNILRGDIGILYSKALITSKNSRGEVFTLERLQ
                     SIIKDNRMKMPSDIATIIQDEFKAFLGISSPESDIVVILFKAY"
     assembly_gap    14232..14331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14332..14716
                     /locus_tag="PLJ75_05535"
     CDS             <14332..14716
                     /locus_tag="PLJ75_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04712.1"
                     /translation="GNQDSLIEMFITTPTLMDTNNEIVITGEKTFRENLGLLQAIKVN
                     NYTLLYLAIGLTILQAIGLFVYGIYITHKVAGPIYVMRHYCKEIQNGNTPQFRKLRGG
                     DLLVDFYNDFITAINTLQDKDASKS"
     gene            complement(14817..15473)
                     /locus_tag="PLJ75_05540"
     CDS             complement(14817..15473)
                     /locus_tag="PLJ75_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPD04713.1"
                     /translation="MNHIPIYKLSEFYDDILDAHEKEEIKSHLDQCPECQKHFEQLHT
                     MVNLLSCLQTISLKRESFALDTIQKIKKHHAHKRYYATMRGSFIAAIILLAIITFLPE
                     SIKHDTSFENIAKISNSQNDQLKLDCIIPVTMDFNTALLLLQQNNARILHSSESYIIA
                     ESDVNSFSSIRQMINGSDSYRKNILSDVAVNHTNKYNKHYPQLTHANKKKYIFRLQLR
                     "
     gene            complement(15470..16033)
                     /locus_tag="PLJ75_05545"
     CDS             complement(15470..16033)
                     /locus_tag="PLJ75_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD04714.1"
                     /translation="MSEYSDNELIDRVIAGDTNAFTDIVKRYQNKIFRYIYTRVYNYD
                     EACDMTQDVFVITMESLKTFRRESQFSTWLFSIAVNYCKNYRRKHRHKIYSINNADDE
                     MEFQIPDLRQNQEEMAITNESMQILLEELHKLPDDYRDILILRDIEGESYTTIAQMLQ
                     LTLANVKVRIHRGREMLKSRLEKRGLI"
     gene            complement(16081..17298)
                     /gene="tyrS"
                     /locus_tag="PLJ75_05550"
     CDS             complement(16081..17298)
                     /gene="tyrS"
                     /locus_tag="PLJ75_05550"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD04715.1"
                     /translation="MIKNDIELLKRGVEEIIPMDEFEKKIEHSEKENKPLIIKAGFDP
                     TAPDIHLGHTVLLRKMRHFQKMGHTVIFLIGDFTAMIGDPSGKSQTRKRLTREEVLKN
                     AETYKKQVFKILDPEKTIIDFNSRWCMPMTFADVLDLASRYNVARMLERDDFAKRYKE
                     GIPISILEFLYPLIQGYDSVALKADIELGGTDQKFNLLVGRDLQREYGQEPQVILTLP
                     LLVGLDGQQKMSKSLNNYIGIDEDPQQIFGKVMSISDELMFLYFELVTDVPELEIDGF
                     KKGIQDGSVHPKDVKVRLAKEICAQFYNEKIAEEAKLEFDKIFVQKDLPSDMPEYRIP
                     ESEKKNNTIWIVKLLVLSGMAKTNGEARRLVEGGGVYIDGNKVENPDAEILCDNPFII
                     KVGKRRFVKIILS"
     gene            complement(17742..18236)
                     /gene="ispF"
                     /locus_tag="PLJ75_05555"
     CDS             complement(17742..18236)
                     /gene="ispF"
                     /locus_tag="PLJ75_05555"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPD04716.1"
                     /translation="MNYQNYRSGIGFDAHRLIEGRKLILGGVHIPFDFGLSGHSDADV
                     VTHAIIDALLGASSMGDIGRLYPDTSQQFKDTYSIDLLHNTYLLLLKNNITIINIDSI
                     ILCQKPKIVPYIEEMKNKLSMACGNMPVANISMKGKTTEGMGFTGTGEGIAALATCLI
                     YCNK"
     gene            complement(18273..18572)
                     /locus_tag="PLJ75_05560"
     CDS             complement(18273..18572)
                     /locus_tag="PLJ75_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04717.1"
                     /translation="MEINLEKKGNTIDIKVKGDIEMMTIKEFKEKLLSIGEENDIDIT
                     LDLSEVDYIDSSGVGVLISLNKIQKKKGKKLELAKVSPKVLNVLKLSSLSEVFNL"
     gene            18858..20735
                     /locus_tag="PLJ75_05565"
     CDS             18858..20735
                     /locus_tag="PLJ75_05565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD04718.1"
                     /translation="MQLKKMHNDIYQSKKINFNKLYEIIKPGSRLFLSSGPAMPICTV
                     EQMIKSDATSLQDLEIIQLITLGDYLKTGEHQYKYRLKTFNTGESISKDIEQGKADFV
                     PAHLVAIPFIFSSRAIGVDVAIIQTSPPDHRGFMSLGIAIDIANIVIENAKIVIAEVN
                     PHVPITMGDTAIHIDAIDYILESDVPLIERELKQYDPMMDKIGWHVANLIDDGSVVAL
                     HVGRQFDAIAQHLHARRELKVYSHVVSDWIIDLIESKALKHGRGINNLAPVTLSYCYG
                     TRMLYDYVDHNPNFEFLPLMRLTYPTILQRIPKLVSVMNVKKIDISGESVVFYSGDNL
                     LSGYESKLNFAVGAAFSRGGKAIVALRSVDQHGDSNIVIRHTETEQVRSMLGVTRYVV
                     TEYGVANLFGKSIRERALALIDIAHPNHRIKILNQAKEAGLVYPDQIYVIKNIINYPI
                     TLETMKTFKDGLVVKFRPIKPSDEDMMRRLFYQFSDESKYLRYFARIRTMPHKQMQSY
                     VNIDYDKILSIVGLIEHAGTERIIAEARYAYDENDKSYEMAFIVDEEFQGKGIATFLV
                     RYLMKIAKERKIERVTANVLPQNDKMIKVFEKCNVPYTQRYDDGVMHFDFHLKKAEIV
                     L"
     gene            complement(20763..21092)
                     /locus_tag="PLJ75_05570"
     CDS             complement(20763..21092)
                     /locus_tag="PLJ75_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04719.1"
                     /translation="MSISSSPSVYTSDNNIQISNAPFTDGNSPTTPTLVMFIALPAFT
                     LIYGVSTWDWGDQKSFHVNRKGFFGENTNAGGANKTGHAFSHYLAFRALYNWKQFAFL
                     LMDSLQK"
     gene            complement(21154..21795)
                     /gene="thyX"
                     /locus_tag="PLJ75_05575"
     CDS             complement(21154..21795)
                     /gene="thyX"
                     /locus_tag="PLJ75_05575"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPD04720.1"
                     /translation="MEIYSSTVTLLAITPNAEKLIEEAGRTCYLSMDRITEGSEANFI
                     RRCIINGHHSILEHASASFRVLGASRAFTHQMVRHRLASFSQQSQRYVNEEEFNYIIP
                     PSIADNGESKELYMQFMDNARKTYKKLRSMHIKKEDARFVLPNALESQIVISANFREW
                     RHVFSLRLEKAAQWEIRNVCIQMLAILQKEAPSVFGDFIIDEATCTARSNIVK"
     gene            complement(21870..22472)
                     /locus_tag="PLJ75_05580"
     CDS             complement(21870..22472)
                     /locus_tag="PLJ75_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009424153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HPD04721.1"
                     /translation="MEIKILFTILISTIFINNYVLSRILGLCPYLGVSKKLDSAIGMG
                     LAVIFVMTMASFFTFIIYKLLLLPYHVEYLRTIAFIIVIATLVQFVEMIIAKISPSLY
                     QALGIFLPLITTNCAVLGVAVLNIDSGYITQQYGLLYSVTQGFGGGVGFTLALIIMAG
                     IRERLELAFIPENLKGLPITFITAGLMAISFLGFSGMKLF"
     gene            complement(22492..23103)
                     /locus_tag="PLJ75_05585"
     CDS             complement(22492..23103)
                     /locus_tag="PLJ75_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPD04722.1"
                     /translation="MNNYFKEIFKGVWEKNPTFVLGIGMCPTLAVSTSVSNAIWMGIA
                     TTFVLLCSNILISTIREYVPSHVRIPIFITIIASFVIIVELTLKAYQPSVYKALGIFI
                     PLIVVNCIILGRAEEFASKNTILHSILDAIGMGLGFTLNLTVLSAIREILGANKLLGY
                     QLIPGMKPALLMVLAPGAFFTLGLLLWGMNVIKARAALFKRTE"
     gene            complement(23100..23780)
                     /locus_tag="PLJ75_05590"
     CDS             complement(23100..23780)
                     /locus_tag="PLJ75_05590"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPD04723.1"
                     /translation="MNTFQMIKAILVLTIIGFIAAFSLSFMNKLTSEPIAKQDQEKKL
                     LSLSLVLPSYTIQKENTVTIDGQPFTYWEASKEENSVIKKAYAFITEEKGYGGTIQSI
                     VGIDDTGKILGFTILSQSETPGLGARCTEITSNETFFDHFFKSNITNTEAIQWFQVQF
                     QGLDTNKPINILKKGDWNNTIKDALLQQNAITAITGATITTKAVTLSIEKGAQKLKKA
                     LTLHEVKQ"
     gene            complement(23829..24911)
                     /locus_tag="PLJ75_05595"
     CDS             complement(23829..24911)
                     /locus_tag="PLJ75_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPD04724.1"
                     /translation="MEIKDADKKRLLEDLVLSSPPHIKDTQSVPLVMWLVVLSLMPAT
                     IFSCYLYGLYVALVIVTSIISAIVSEIIMQYLLKRPITVNDGSAVITGLLLAMNLPPH
                     VPLWIPAIGSAFAIIIVKQLFGGLGYNIFNPALAGRAFLLASWPVEMTTLWHRFPNGY
                     VIAQNPIFNAAMPQQLIDSISGATPLGALKEAPKLLQELSLQAQPLYDFLFSNAMIKS
                     LFIGNIGGCIGETSAILLLLGALLLLWKKVITWHIPLSYIATVAACMGIYYYFTGVPN
                     LHYAILFHVLSGGLILGAFFMATDMVTSPVTKTGMIIFGIGCGILASIIRLWGGYPEG
                     VSYSILIMNGITPLIDKLTRPKVFGA"
     gene            complement(24948..>25887)
                     /gene="rsxC"
                     /locus_tag="PLJ75_05600"
     CDS             complement(24948..>25887)
                     /gene="rsxC"
                     /locus_tag="PLJ75_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPD04725.1"
                     /translation="KVQSAGIVGLGGAAFPTAVKLNPPANRTIDTLIINGSECEPYLT
                     VDDMLMQTHPEEIIEGIQIAMKILGINKAYLGIEKNKPLAIKALQTAVANVKTHEIVI
                     IPLNKRYPQGAEKQLIYTITKREVPSGGLPMDVHVVVQNVGTIYAIREAVLFDKPLIE
                     RYITVTGKIVRKPGNYKVRFGMRVSDILEECGGLVENPSKIIMGGPMCGLALNHMDIP
                     VVKGTSGILFLAKNETKVTPFRSCIRCGRCVSVCPMNLIPCDLGNAAEKQRYDIAEKL
                     HPFDCIMCGSCSYICPSKRPLSHFIKVVQTTLSAKR"
BASE COUNT         8095 a         4501 c         4942 g         8049 t
ORIGIN      
        1 tgacataaaa aatcttattt ttactatcgg taattgaaaa aaaatataaa ttaaaaaata
       61 tttttttaaa atggccattc cggcgttttt atatataggc gatagttact tttaaaatag
      121 ttgagtaaca gcataaatga tacggagttg cccatggctt gattacagct ctccgtaatg
      181 aataaaaatt atacaaaccc aaaataatgc ttctctcgac ttgataagga gaggtgcagg
      241 aaatgaggtt aatatttcta ttgcaggagg atgctatgca gtcaaaaaat gatctgccca
      301 tacaacggtg tataaaaggt gaaccacttg aagcattgag cgatgttgag cttttagcag
      361 tactaattgg cagtggggta aaagggaaaa atgttatgga atgtgcgttt tatgtcattg
      421 aacaatttgg agggatgaac gggatatatc atgcagggat acgagaactg gcacatacag
      481 atggtattgg atttgtgaaa gcaattcgta taaaagctgc attcgagtta gggaggaggt
      541 taatagtacc aaaaaattat gaagggtcaa ttgactcacc gcgtatggta tgggaattca
      601 ttgtgggaca gattgcatgc aataagcagg aggaattctg ggcgcttata ctggataata
      661 aaaacagatt gctgaaaaaa acaagagttt ccataggtac tatctctgaa tcaatagtac
      721 atcctcggga agtcttcaga gatgcaatcc gcgaagcagc atcatccatt atcattgctc
      781 acaatcatcc gtcagggttg cttaagccat cggtcaatga tattgagatt acacgcaggg
      841 tagcacaggt tggtgctata gttgggattc agcttctgga tcatgtaatt gttaccgata
      901 acgattatct gagtttgcgt gaaactgatg aaaagttatt tttggcataa tgctatatat
      961 aaagcggtgg tcagtaggct ttgttaaaat ataatcatac agttacatag aaaggatata
     1021 gtaaaacagt tgacagcagt tttaaatatt tataccataa ataatgactt tattatggaa
     1081 cagtcatagg agtattgcca tacatcccgg aagaaccaac tatgaaagca atccagcatg
     1141 tagcgtccgg gattttggtc ctatgaataa ataatcagga gaacatatga aatacaaaaa
     1201 accgggtact attgtgttga ttgccattac cgtaattttt atacacgcaa cacttgtttc
     1261 tgataccagg catggtacat gggttatcta taatgtgccc gcttcaccac aatccgaaaa
     1321 aacaataccg gttaagattt actttcctaa aagctattca gattcaagcc gtgttattat
     1381 cgcatttcat gaatatgacg gttctatgaa tagctggcag aataacacca gcattgccta
     1441 ttatgccaat caatacaata tggtcattgt gtgcccacac atgccacgct cagtttacga
     1501 atcatattat tttgatcaaa ctacggttaa atggaatgag atgccggcgc ttttgtggat
     1561 tggccagtta ctgctgccct atttaaataa tactgttggc ttaaacatta caaagcaaaa
     1621 acttggcgtg tgtggttttt caattggtgc gcgtggtgca ttgcgcgtgg gtgaggtgta
     1681 tcatgacttt gttggcgcta ttgcatgttt gtcaggatat tacgatatgc tttcgcatac
     1741 aaaaaatcat atgtttaccg ccgtctatgg aaaatataat gactttcaac aacgatgggc
     1801 acaaactgat aatgctattg atgaagcaaa aaatcttggc gatgttgcag tgttcattgc
     1861 acatggcaat aaagattcgc gtgttcccat ggagcaggcg tttatgctgg cgttaaagtt
     1921 gaaacagctt caaaaggaat ataaaagtgg ctataatttt acatttgtgg aaaaaaagta
     1981 taagatgcat gattggaatt actgtcagtc ggtgcttccc gatatgatgg cattctttaa
     2041 tgaaaattta aaataaaaaa atatatgaaa aatttcaggg cgatagttcc ttttatcatt
     2101 gtattggtgg cattgtgttg cagtaagcat ggtgtccgca gtggagccat gctatgcaca
     2161 atgcataacg ctcataattt ttatagtaag gctgattttg cacgatgtat gggaatagat
     2221 gaaaatgcac ttgaggatta tttcattttt tctcaatcag caaaagcgtt cagtgatact
     2281 gcatcaaccg agagtatgct aaaatcaact gtttgcgata caatagtaga tgaggtaatg
     2341 ctgcgcatac agactatgtt taccaataag cagtttgcac gatggcattt tactgagatg
     2401 tcattgccgg tacagttcag gacatttata cagcagggaa gtatccctga aatgatggtt
     2461 gttggattgg taaaaaaaga ggacctttct gccagggcaa ttatatatta tttgccatta
     2521 gaatataaga tggacataat aggaagagaa aaagaaaacg gcaaaatgcc tgattggtga
     2581 ataaaagctt gacaaataat gagcgaacgc ttgttttata aaaaaaggat ggtannnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaataaa agcttgacaa ataatgagcg
     2761 aacgcttgtt ttataaaaaa aggatggtat atgcttacac tgggtatgga tataggttca
     2821 attactgcaa aggctgttgt tttaaatggt ggcactatag ttggcagtaa ggtaattttt
     2881 acagggttta atgctgcaaa agctggcgaa aaggtggtac aggagctttt agctgaatta
     2941 gggctaacac tagaacaaat tgaaaggatt gtagcaaccg gctatggtcg gaaaagtgtt
     3001 ggatttgcac atgctacggt aaccgagatt acctgccatg ccagaggagc tcacttctta
     3061 gataactcag tacgatctat tatagatatt ggcgggcagg acagcaaggt gatagttatc
     3121 gatgaaacag gcagggtaaa ggattttgcc atgaacgata agtgtgctgc cggtacagga
     3181 cggtttttag aagtaatggc acgtgcgctg gaggttgatc tggatgagtt tggagaacta
     3241 tcgctcaagg cagataatcc gtcaaagata agcagcttat gtacggtatt tgccgaatcg
     3301 gaagtaattt cacttattgc aaagggtgag tcgcgtgata acattattgc tggtattcac
     3361 cagtcaattg catctcgtgt aatggcaatg gcatcgcgtg tggggattgc cagtcctgtt
     3421 atgatgactg gcggtgtggc aaaaaatatt ggcgttgtca atgcaattga aaagttactg
     3481 aattccccgg taaaagtttc aaaggatgcc caggttattg gtgcacttgg tgcagccata
     3541 ctggctgctg aaaattagtt atttcttttt gctggtaaaa agcctttccc agtattcaat
     3601 ctctttttga ctcaggttag gattttcttc tttttcaggt gtttgagcaa gggtgaaatc
     3661 ttcaatggtt tgattaacaa acagagcaaa gtcttcgctt ttcatagttt ttgttttaaa
     3721 acggctaaca tactctatta tatctttgtc gctggttacg acagttgtgt tgcgtggctg
     3781 taagtctttg cgtataaatt cctttattaa aaagtcagca gaataatgca gggaatagta
     3841 cacatcgata gtatatactt tttcgcttga gataggaata tcactggttt tttgtccgtc
     3901 aaaaacaatg cgtattcgtt tgcgtgttag tttggcaaac atctttagta aatctaaaag
     3961 ctcacggcgt gcttctatga gattatcctg aagcatgagt tcttctaaat ggggaaattt
     4021 atatatgagg ttatagccgt caatcagata cgtcatgaca cactcttgca aactggttta
     4081 ttaactggaa attatgattt tcacactata tacaaactat agtactatac aagcattttt
     4141 tttaagtaac tatcgccata taaaattttt gtataattta gttgactgat gcataatcca
     4201 atgtataaaa atataaaaaa ggtatagtac agctttctta acaccgacat acagtataac
     4261 aattgaaaaa gggatagtat gactgatagt aacaacgaac aaaagtacaa cgttgcatac
     4321 ttagaacgtc agctctttaa cctgtcaaag cttgttgaaa ttaacaggat tattaactca
     4381 acacttgaca tggcaaaact ttttaccata ataatggaaa tcattaaaga gataatggag
     4441 accgaagcaa gcaccttgtt tttgtttgat gagaaaacca atgagcttgt gtttaaagtg
     4501 gctctgggtg aggccggtga taaattgcag gagaaataca gggttgcgat ggggcagggt
     4561 attgcaggtt gggtagcaaa agagcgcaaa ggcgtgtatg ttaacgatgt ttatctggat
     4621 gaacgctttg accccaattt tgacaaacag actggattta ccactaattc aataatatgt
     4681 gttccacttt tgtataaagg aaagcttctg ggcgtgatac aggcaatcaa tccaatcaac
     4741 cgtcctgatt tcactgatga ggatatgaat ttgttcagag catttgctga tcaggcggtg
     4801 ctggctgtgc agaatgcaat attctttgag cgggctattg aagaggcgcg catgcgtaat
     4861 gagctttctg ctgctcatga gatacaacat tcattgcatc catctattgc aaatgattat
     4921 gttttttgtg agatagctgc tcgttatctt cctgcgcgtg aggtgggtgg tgaattttat
     4981 gaaataaaac catatgatga tgcagttgca attgcagtat gtgatgtaca tgataaagga
     5041 gttcccggag cgctgaatgc cgcattggtt aatggtttgt tgaaaggatt aatgaatgtt
     5101 tatggtagag ttgtggcacc aatcttttta aatacgctgc aggttgttgc ttcacatcat
     5161 atcaataatg tatcgttatt tttgggtatg attgataata aaacaaaaac tttaacattt
     5221 atgaatacag gcgaggcata tcctgttatt atacgggata gtattgcaca ttatttgcga
     5281 ttttcacatt cgctacataa taataaaaag attgttgtaa agcttaagca tggggatatg
     5341 gttgtcattg tcaccgatgg agttgtaaat tgcaaaaatc gtaatgcacg actttttgga
     5401 ttaaagcagg tcatggacac atgttctcaa ttttcaactc cggaatcttg cattgctgga
     5461 cttcttgata ctgtcaaaaa ttttatgcag ggtttaactc aacgtgagga tatatcggtc
     5521 attgctgtaa aagtaaaata aaaaaaagag gcatcagatg gaatatgttt cagtgatttt
     5581 gtgcaggaat tgcggttccc gatacgttga agtgaatgag tggacccagg ataaaaaggc
     5641 tgtattccac tgccgtacct gtggcaaaaa agaaatagtg gaatggttta cgctgggccg
     5701 atgtcaggtt acaccatcag aattacaaaa aacacgtgat acaaaagcta aaccgggtaa
     5761 atatgaaaga tagttattgc cgtatttttt gccatactac ctgtatataa aataataaca
     5821 atcgcaataa acgcagataa cgccatggac gggttataat gcggtaaaac cagtcaagac
     5881 ctttttccat aaaaaaagcg ggagctttat gtttttgccc ggagataata tcaatacttc
     5941 caccgattcc aataaacaca gtattcttaa actgattttt aatttgataa atccatttat
     6001 cttctttggg gaaaccaagt ccaaccagta taatctgtgc ttcggatttg cgcattgcct
     6061 caatgacatt ggaagctctt tctttattga agtaaccgcc atgacgcccc acaatgcgta
     6121 tcattgggaa tgactttttg atgttggcat atgcctgttc aataatttct ggttttgcgc
     6181 ctaccatgaa tattgtaatc tcttttattt ctgcaatgcg gatgatatcc attaaaaagc
     6241 tcacaaaatg tattcgctct tttaaaggtt tgcctaaaaa tgatgctgcc catgggagcc
     6301 ctgcccaaac agctattttt aaatcagctt ttgaggcaat gagatttaag tcattgtttg
     6361 taataagacg gtgaagtttg tatggattta acgccattac atgatgcaca ccgccttctt
     6421 cgatcatacg caacacctta acaactgcct gctgacgcgt acaattgtca atacctatgc
     6481 caaggatatt gactaaatta acatctgcaa gtgaagtctg attatattcc agtattaaat
     6541 cgcgatcttc tttatacgag tcataatata ttgcgtcttt aatcattaac tatcctctct
     6601 taataatata tatttgtggt tttccctgcc ctcacttctt tgactaatag aatgtctttt
     6661 ctattagaac aaaacggtat atagggtgtg ggatagtagt tataaagcca aataccataa
     6721 aatggccact gtgttgagta ttagagaaga taaacgatag agtatggatt atgtcaataa
     6781 aaaattatta ttataaaaat agtaactatt cagctgctcg gaaataaatt caagctaaac
     6841 ctgtcaccct taagaatacg ttcgagtgca tcaataatac taaaatactg ttttttaact
     6901 gttgaaatga aacttaccat aacacaaaat tgttcagtgc cgctaaccgt tctgaataat
     6961 cctgaaattt tctgttgcat tttaagcatg tatatatttc tttccgaaaa attgttatta
     7021 agcggtaatc catatcaggg ggatatcaaa ttcctgccgt atattatggt tttttatcac
     7081 tgttacttaa ttggttgcca caataaatga ataattatgc aaaaactatt gaatatatat
     7141 tcgtggcact cgagggctta tgtttgttaa cacttcgtag gtgatagtcc caattttttc
     7201 acacagagat tccagtggga tggaatatcc atccatttga ccaaacagca gaacatcatc
     7261 cccattatac gccgttccat cataaccaat atccaccatg agttgatcca tgcatatagc
     7321 gcctaccaca ggataccttt taccacgaat aatcacctcg ccttgattgg aaagcaatcg
     7381 gctataaccg tcaccatatc caacaggcaa tgttacaacc cttgtttgcg ttgtggtttt
     7441 ataaaggtga ttatagctta ttccggtatt agcaggaaca accttaaaat atgaaacttt
     7501 agtttttaat gtcataactg gcaaaagctt tttcctatta aattcaattg atggattata
     7561 gccattggga ttatagccat acagcatgat gccaggacgt accatggtga agtgagattc
     7621 tggattattt attattgctg cagagttggc aagatgcacg tatggcggca gaattgactg
     7681 tctgtccata tagttaacaa tgctttcaaa gcgggtaatt tgttcttgag tgaaatggct
     7741 gtcggattca gctttagcta aatgtgaaaa aataccaaca acctgaatat ctggcagttg
     7801 cagtgtttcc tcaataaatt tttgagcgtt gtaccagtgg acaccaatgc gttccatacc
     7861 ggtgtcaatc tttaagtgta ccagcgcttt tttgcccagg gcttttgctg cagtttgtat
     7921 agcttttgat ttatcaatgg aagagcttgt tattgcaaca tcgtttatga taaattcggg
     7981 gatttgttct gagttaatgg cgccgcaaac aagtattggc gtatgtatgc catgctggcg
     8041 caaaaacagc gcctcttcca gataggcaac acctaaatag ttaacaccag cttttataag
     8101 ttcctgagag atgcgtacaa taccatgtcc atatgcgttt gcttttacca tcgccatgac
     8161 ttttacttgc ggtgaaatga gcgagcgtac aattgcaaaa ttattcaata aattgggtat
     8221 tgatatttca gctcgtgttg gtcggttgca atatgcagga tttgttagta tcatagttta
     8281 aaatctatag cgtatagttt taattttaaa attttgccat atacagataa aatcatttta
     8341 accatcaaaa atttaaatca tgaaatatta aaaaagaatt atgatgaagc tatacttctt
     8401 tagcagttat ctaattctgt caagatattt ctaaactggc attgttgtaa attgcatgat
     8461 gaacaaacac tatgactgtt accataaggt aatgcaaaac ctgctacggt gtgcagtggt
     8521 tgaaacatgc aatgtcgatt taatgcaata cctatatttt gcgcctgtgt tttaagcatt
     8581 gcatatatgg catggttgag tgttacaggc agttcttttg atgagccagg ataccatacg
     8641 tagccgccag ttccaaacag cttctttaat aattcctgca catattggtt gcaaagccac
     8701 atcccgatta ttgatgcatg gtattgatgc gttagtccgg gtaagcttat gtaccagttc
     8761 tcatatcgta tccccaaggt aaagatgaag gcatatgatc tatctgaggt aactatcgca
     8821 tcaggtatag caatggtact gttgattgca tttgctttac caatagcagc ttcaggcttt
     8881 gctatgtgat atgcctgtac ataaagttga tatattgaat caggcaatgc tgagagcaat
     8941 tgattattgg acaaactatc gccagaaaaa gtatttatgg tattaatata tgcatcgctg
     9001 cctttgtgta agctgtgcga tagttcctga caatcagttt tctgatgcaa aagcttcagc
     9061 aggctgttat caacgggaaa agctatagtg tcaaaggtga tgattttact ttgtgtcata
     9121 gacatataaa aatgcaggtg acgaggggct cgaaccccca acctgcggtt ttggagaccg
     9181 ctgctctacc aattgagcta gtcacctgta tattaagaac aaagaaaaat tatattcgtt
     9241 atgtcaagcg tttttaataa tattcaccac aggcgacaat atcatcaaaa agacattttt
     9301 gataatcttc ggattcctga aattcaattg ggtagttgcc gtcaaaacag gcagtacaaa
     9361 atttgccatg gtggacgcca agagacgcaa gcattgtttc aacagtcata tatgcaagcg
     9421 aatccacacg cagatatttt tgaatctcat caatggcatg tgaggatgct attagttctg
     9481 aacgtgttgg tatgtcaata ccatagaaac aggggaaacg agtgggaggc gctgagatac
     9541 ggacatgaat ttctttagcg ccgccatttc taaacatctt gatgatcttg cgcatggttg
     9601 taccacgcat tatagaatcg tcaacaacaa cgattcgttt accttctatt gctgagcgaa
     9661 caatgttaaa tttaattttt actccaaagt cgcgtatacg ctgttcgggt tcaatgaatg
     9721 ttcggccaat ataatggctg cgtatcattc ccatttcata gggaatgccg ctggcacgcg
     9781 agtaacccag tgctgcacaa actgaagagt cagggatggg aaccactata tctgctttta
     9841 ccggcgattg ctttgctaac actgctccca ttctaaggcg catctcatgc accgattgtc
     9901 caaatataat gctgtcgggt cgtgaaaagt agatatattc aaaaacgcac aatgaatttt
     9961 taacacttcc aaacgggaaa tatgatttca taccattggg tgatatctcc accatttcac
    10021 ctggttcaat ctcgcgaata tattcagcct caataatatc aaatgcacag gtttctgaag
    10081 caactaccat ggagttgcct aatttaccaa gaacaagcgg tctgaaacca taagggtccc
    10141 gtatagcgta tatggtgtta ttgttcatga caagcaacga gtaagcacct ttaatagcct
    10201 tcagagcata aataagggca tccagaaaat tggtaagtcc tgatcgagcc atcaaatgga
    10261 ctattacctc gctatcgatg gatgtctgga agatggctcc ttccttctca agagctcttc
    10321 gcagaatggc atagtttaca aggttgccat tatgagcaag aactatcggt cccagcactg
    10381 aatgaacacg aagtggctga gcattgcgta aaaacgatga gccagtggta gagtagcggt
    10441 tatgccctat agctatagta cctttgagat tattgatatg ctctttggta aatacatcta
    10501 caaccttacc catgccggca tagcgataga tatgctcgcc atctgatgag gcaataccgc
    10561 ttgattcctg cccgcgatgc tggagattgt aaagtccaag gtaggtaaga tttgctgctc
    10621 tggggttgtt gtaaataccg aaaacaccac attcttcacg aggcttacaa tcacaatact
    10681 catattctgt tttcatattt ttcctgaaaa atggttagat taagttatga tgagaaatgg
    10741 ttataataca ccctgacaat agactgtaca taaaaagaaa caataacctg attgtcacta
    10801 ttaattcata cacaattaca aatataattg gcttttatat aaaatcaata aaaaattaaa
    10861 ttaaaaagtt agtattgcta tggtacgtgg ataatcagat gtatggtaaa attaactatt
    10921 ttaaaaatta caataatctg cttgaaatca taatacagtg tcagtaccat cggcgatagt
    10981 tactaatgaa tagttcattg cattgataga caatctaagg ctaatgattg acaaacggac
    11041 aataacagag agttgtgcgt ggcggtatta taattatcca gatggaatga aaatannnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatgtg atagtctttg ataattttcc
    11221 aacaggtaaa gaggaaaatt tatcatttat taaacaatat cccactgcca ggataaaagt
    11281 tatacacggt gatgttgctg attggtatgc gttgcatgaa gctgttaaag gctgtgatgc
    11341 tgtgtttcat gaagctgctg ttgcttcggt acagcaatca atagaaaatc ctccggcaac
    11401 atttgaatcg aatgcaaagg gaacgttaca tgttcttgaa gcatgtagac aaaatggggt
    11461 taaaaaagta atatttgcct gttcagcggc tatatttggt gatcatccag aaattcccaa
    11521 gaaagaaaat tctccggttt tcccaaaatc accgtatggt gcagataaat atatttcgga
    11581 ggtttatctt cgactgtata atgagttgta tggattaaca acggtatcgc tccgttattt
    11641 caatgtgttt ggtccagggc aggatccttc gtcaccatac tcgggtgtta tttcaatttt
    11701 tacaaaaagg atagctatgg gtaaggatat cactgtatat ggtgatggaa cgcaataccg
    11761 tgattttata tttgtgaaag atgttgtaca ggtaaatatg ctggctctgg aaaaactgga
    11821 caaaggtttt cattattaca atgtgggata tggtaaagcc actgatataa attccctgat
    11881 aaccatttta caaaatattt ataacaaaac tattaaagta cattatgttg aagcaagacc
    11941 tggtgatatc aaagaatcaa taagtgaccc ggcaaaggta atcagggagt taggattttc
    12001 tccacagtat agtgttgagg aaggattaac actcatggcc ggtgtcatgt aattaatatg
    12061 ctttaaatag tatcacaaca atatcagatt caggggagct tatacctaaa aatgctttga
    12121 actcatcctg tattatggtt gcaatatctg atggcatttt cattcggtta tcttttataa
    12181 tagattgcag acgttccagc gtgaagacct cgccacgtga attttttgat gtaatcaatg
    12241 cttttgaata taaaattccg atatcgcctc gcaggatatt tgttctgccc ataccgtagt
    12301 taaacgttgg atcatatccc aatggtatcc cttcagtgtc cagtgatgaa aaagcacttt
    12361 tttcaactcg gtacaattcc attggcggga aacctgcgtt ggtataaata agacgcatat
    12421 tgcgggcatc aataaagtag taataagcag taatcaaaat tcccttccct tgcgaataat
    12481 tataaattac cttatttaat gtctggatga tattgtatga agaatgtgcg tcgcttagac
    12541 ttgcatggaa tgctgaacgc atgagcaccg aatacaatgc actttgaata cctattccgg
    12601 caatgtcggt ggcgatagtt cctataccct gtaatccagg tcgtatgaag tcaaagtaat
    12661 caataccacc gccctggcgc ggtatataga atgcaccaaa tttgattccg gtaatgtttg
    12721 gcaatgcttt tggtacagct tttgatagca gatcgcttga aacggagatt gttctttgca
    12781 acaattgttc ttttttagct tcctgaaatg ttatcgcatg agaaatattg atatttgttt
    12841 cgctggaaag aaattttaaa agttctatct catcaggttt gtatgatgca aacgtatctt
    12901 tttcacctaa gcatagtgcc cctactaaaa cccttcgatg atatatgggt atcatgataa
    12961 caatattatt tactgtaaaa tattggatga attcatcacg aacatctttg aatgatgctt
    13021 catccatgta cacattatct atagtaacta tcgtttgatt acggttaaac cagcgtacaa
    13081 ttggcgataa ccgcggtatg gagggagata ccggcgccat gcccatagag tagtttattt
    13141 gatagctgcg tgattcattg tcatacatca gaatataggt tgtttttaag aagaaagtgt
    13201 tgtggatggt tgaaacagtc ctttttaata attcatcaat atctcgtaga ttgctaattt
    13261 ccctgacgaa ggtatcaagg tattcctgta atgcatagtg cttgcgctta aaagctttat
    13321 caatgacggg ctgaacatat tttgaaagga tcataaaaac aacaacaata accagagcta
    13381 tcacaaatac aggaatattt ttcaacccat agatgttaat gtcatagatc tgtagcagtg
    13441 caaatatggg aaggaatatg accgcggaaa tgatggcgta catggtggtt ttgtgtatgg
    13501 cggttcgtat atccatcagt tgatatgaaa tgactgcata gaacagagat gatactccaa
    13561 tgaatgctgc aagcgatggc cctaaaacat agaggtcaac aaaattaaag aaacgtggca
    13621 tgataatcga aaatactgca gcaaatgtca tggcaatacc tgtaccaata aatacaaacc
    13681 tcatctgctt acggtagaga tctatttttg tagttatgta ctttcgtatg aagtttataa
    13741 cccctaatag tatataaaca aaagaaacaa ctgaatatat ggtataaaat ggcttaaatt
    13801 cacgcaccaa ttccccatcc ttgaaatatg cagcggtgat atttaaatcg gtgaatactg
    13861 ctatataagc aaagatataa gcagggatta agattaatac aataatgaag aaagggacct
    13921 gtttttctgt ttttggaaat attaatgata atacaaatag tgcggagaat gttagtacag
    13981 tggaagcctg cgttatctta ttaataaggg taagatcaag tttatcagga aatgcgtagg
    14041 ttaaaaacat ggtaaaaagg ataccaaagg cacaaaaacc aaaatacgag tagtagcgca
    14101 ttaccctatc ttgaaagttt ttatataaac cgtaaacaat agtaaatatt agcatacatg
    14161 atgctaaaaa cgaacctatt aaacttaagg ttatcatata catagccctt ttgtatgtat
    14221 ttttatatag tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggtaatca
    14341 ggatagttta atagagatgt ttataacgac accaacattg atggatacca ataatgaaat
    14401 agttataact ggtgaaaaaa cgttcaggga aaatttaggt ttattacagg ctataaaagt
    14461 aaataattat acattattgt atcttgccat tgggctaaca atacttcagg caatagggct
    14521 gtttgtatat gggatataca ttacccataa ggtggcaggc ccaatttatg ttatgcgtca
    14581 ttactgtaaa gaaattcaaa atggtaacac gcctcaattt agaaaattaa gaggaggcga
    14641 tctactcgtt gatttttata atgattttat aacggctata aacacgttac aagataagga
    14701 tgcatctaaa agctgaattt tcattatatg aaatttttga aaaactgcat tgttgattat
    14761 ttactgctat tgttgatgca ataaaataat ttttttaaat ataccaaaac aaaatactaa
    14821 cgcaattgta gacggaaaat atattttttc ttatttgcat gggtaagctg tggatagtgt
    14881 ttattatact tgttagtatg attgactgca acatctgaaa ggatattttt acgataacta
    14941 tcgctaccat taatcatttg tctgattgaa gaaaagctat taacgtctga ctcagcaatg
    15001 atatatgatt cggatgagtg aagtatacgt gcattatttt gttgtaacag caatagggca
    15061 gtattaaaat ccatggtaac agggataatg caatccagct ttaattggtc gttttgtgaa
    15121 ttgctaattt tagcaatgtt ttcaaatgat gtatcatgtt ttattgattc gggtaggaat
    15181 gttattattg ctaataaaat gatagctgca ataaatgatc cccgcattgt ggcataatag
    15241 cgcttgtggg catggtgctt ttttattttt tgaattgtat ccagcgcaaa gctttctctt
    15301 tttaaggata tagtttgtaa acatgataaa agatttacca tggtatgcaa ttgttcaaaa
    15361 tgtttctggc attccgggca ttggtcaaga tgtgacttaa tttcttcttt ttcatgggca
    15421 tccagtatgt catcataaaa ctcggataac ttatatattg gtatgtgatt catataagcc
    15481 cccttttttc caggcgtgat tttagcattt cacgtcccct gtgtatcctt acttttacat
    15541 tggctaatgt tagctgtaac atctgtgcaa tggtagtgta tgactcgcct tcaatatcgc
    15601 gcaatataag tatatcccgg taatcatcgg gaagtttatg aagctcttca agaagtattt
    15661 gcattgattc attggtgatt gccatctcct cctgattttg ccgtaaatca ggaatctgaa
    15721 attccatttc atcatctgcg ttattaattg aataaatttt atgcctgtgt tttcgcctat
    15781 aatttttaca gtaattaaca gcaatgctga aaagccatgt tgaaaattgt gactcgcgtc
    15841 taaacgtttt taacgattcc atggtaataa caaatacatc ctgtgtcata tcacatgctt
    15901 catcgtaatt ataaacacgc gtatagatat acctgaatat tttattctgg tatcgcttta
    15961 caatatccgt aaatgcattg gtgtcacctg caattaccct atcaatgagt tcattatcac
    16021 tatattcaga catagttatg gttacaatgt taccaaaaaa attaattatt tacacgatag
    16081 ttatgaaaga attattttta caaaacgccg tttgccaact ttaataatga aaggattatc
    16141 gcacaatatt tcagcatcgg gattttccac tttgttacca tcaatgtaaa caccgccacc
    16201 ttcaacaagt cgtcgtgctt cgccatttgt tttagccatg cctgataaaa caagcaattt
    16261 tacaatccag atagtattat ttttcttttc tgattcaggt attctatatt ccggcatatc
    16321 agatggaaga tccttttgaa caaaaatctt atcaaattcc aactttgctt cctctgcaat
    16381 cttttcgtta taaaattgtg cacatatctc ttttgcaagc cgtactttga catcttttgg
    16441 atgtaccgac ccatcctgga tgcctttttt aaaaccatct atctcaagtt ctgggacatc
    16501 agtaaccagt tcaaaatata aaaacatcag ctcatccgag attgacataa ccttgccaaa
    16561 tatctgttga gggtcttcat cgatgccaat ataattattc aaagatttac tcatcttctg
    16621 ttgaccatcg agtcctacta aaagtggcaa tgtcaggata acctgcggtt cctgaccata
    16681 ttcacgctgg aggtctcttc caactaaaag attaaatttc tgatcggtac ctcctaattc
    16741 aatatcagcc tttaatgcta ctgaatcata accctgaatc aatgggtata aaaactccag
    16801 tatggaaata ggaatgcctt ctttatagcg ttttgcaaag tcatcacgct ctaacatacg
    16861 ggcaacgttg tagcgcgaag ccagatccaa aacatcagca aaagtcatgg gcatacacca
    16921 tcgtgagtta aaatcaataa tagttttttc aggatcaaga attttaaaaa cctgtttttt
    16981 gtaggtttct gcatttttta atacctcttc acgggttaat cgttttcgtg tttgtgattt
    17041 tccagatggg tccccgatca ttgcagtaaa atcgccaatg agaaaaataa cagtatgccc
    17101 catcttctga aagtgccgca tctttctgag taaaacagta tggcctaagt gaatatcagg
    17161 tgcggttgga tcaaaaccag cttttatgat taaaggtttg ttttcttttt ctgaatgctc
    17221 tatttttttt tcaaattcat ccatcggaat gatctcttca acacctcgtt tcagcaattc
    17281 aatatcattt tttatcatag tatatatcct cctcagaaaa tagaattatt tacctcattc
    17341 ccgacccctg cccttcgtaa ggcgagagaa gcattatcgc agtattgtca ttctgatgga
    17401 gcagtagcga ccgaagaatc tattcgttca caaaacgacg atattcttcg acttcgcttc
    17461 agaatgacag gtcatcacca ctgtcatttc gggcttgatc cggaaactct tttcaccatg
    17521 agatcctgaa ataaaattca ggacatgatt caggataact atcgccaaac aatgctgttt
    17581 gcgctcatag cattgtactg ttagtatctg gaagagtgtc tgcaacgacc gttgaaaata
    17641 taataacaat ggcaaaaatc cattctccaa atgaacaatc aatattgatg gataactgtc
    17701 tgtcaacatg tatttgttga tttattagta agggcgtatc attatttgtt acagtaaata
    17761 agacaggttg ctaatgcagc tattccttct ccagtaccgg taaaccccat gccttcggtt
    17821 gttttcccct tcattgatat atttgcaaca ggcatgttcc cgcatgccat tgataattta
    17881 tttttcattt cttcaatata gggcactatt tttggttttt ggcatagaat gattgaatct
    17941 atattaataa ttgttatatt gttttttaaa agaaggaggt aggtattatg cagcaaatcg
    18001 atactatatg tatccttgaa ttgctggctg gtatcgggat ataaccttcc tatatcgccc
    18061 atgctgctgg cacctaataa cgcatcaata attgcatggg taactacatc agcatctgaa
    18121 tgtcctgaga gcccaaaatc aaatgggata tggactcctc ctaaaataag cttgcgccct
    18181 tctattagac ggtgggcgtc aaaacctatg ccacttctgt agttttgata gttcatctgg
    18241 aataaaaaat agattgttca ggctttatca tatcataaat taaatacttc agatagtgaa
    18301 ctaagcttta aaacattaag tactttaggg ctaacttttg caagctctaa tttttttccc
    18361 tttttctttt gaattttatt aaggcttata agtactccaa caccagacga atcgatataa
    18421 tcaacctctg aaaggtcaag tgtgatatca atatcatttt cttcaccaat tgacaggagc
    18481 ttttccttaa actctttaat ggtcatcatt tcgatgtcgc ccttaacttt aatatcaatt
    18541 gtatttcctt ttttctcaag gttgatttcc atggttaact cctctataaa ataaagtcta
    18601 tatagactaa ttaaatacta cataaataaa tgtgtcaata aataaaatga acattgaaaa
    18661 ctataccttt taataccatt gtgagaacaa agttatggtg ctaaagcttt ttcaaatggt
    18721 aagcccataa aacgttattg gtaaaggttc ccacaccgtg caactctttg catgaatgca
    18781 ttacatcaaa ttgttgacaa aatacgaaaa aaatataaaa tatactattg gttatactac
    18841 cattacgagg taatttaatg caattaaaaa aaatgcataa tgatatttat cagtcaaaaa
    18901 aaatcaattt caataaacta tatgagatta taaaaccagg gtctcgatta tttttaagca
    18961 gtggtcctgc aatgcctatc tgtactgttg agcaaatgat taaatcagat gcaaccagct
    19021 tacaggatct ggaaataatc cagctcatta cattgggtga ttatcttaaa accggtgaac
    19081 atcaatacaa atacagactt aaaacattta atacaggaga aagtatcagt aaagatattg
    19141 aacagggtaa agctgatttt gtacctgctc atctggtcgc aatcccattt attttttcca
    19201 gccgtgcaat tggtgttgat gtggcaatta ttcaaacttc accaccagac catcgtggat
    19261 ttatgagttt aggaatagca attgatattg caaatattgt tatagaaaat gcaaagatag
    19321 tcatcgctga ggtaaaccct catgtcccaa taacaatggg tgacacagct atacatattg
    19381 atgcaatcga ttacatactg gaaagcgatg taccattaat tgaacgtgag cttaagcaat
    19441 atgacccaat gatggacaag ataggttggc atgtcgcaaa ccttattgat gatggttcag
    19501 tagttgctct ccatgttgga cgacagtttg atgctatcgc acaacattta catgccaggc
    19561 gagagcttaa agtttatagt catgttgttt ctgattggat tattgattta atcgaatcaa
    19621 aagcattgaa gcatggcaga gggatcaata atttagctcc agttacattg agctactgct
    19681 atggaacacg catgttgtat gattatgtag accataaccc taattttgaa tttttgcctc
    19741 tgatgcgcct tacctatcca accattttgc agcgcatacc caaattggta agtgtcatga
    19801 atgtaaagaa aattgatata tcaggtgaat cggttgtgtt ttattcaggt gacaatcttt
    19861 tatcaggata tgagagcaaa cttaattttg ctgttggggc agcattttcc cgtggtggca
    19921 aagcaattgt tgcacttcgt tcagttgatc agcatggcga tagtaacatt gtaatcaggc
    19981 atactgaaac cgagcaggtt cgttccatgc tgggagtaac acgatatgtt gtgactgaat
    20041 atggtgttgc taatcttttt ggaaaatcta ttcgtgagcg agcattagct cttattgata
    20101 ttgctcatcc caatcacagg attaaaattt taaatcaggc aaaagaagca ggattggtat
    20161 atcctgatca gatatatgta attaaaaata tcattaacta tccaataaca cttgaaacta
    20221 tgaaaacgtt taaagatggg ctggttgtta agttcaggcc tattaagcca tctgatgagg
    20281 atatgatgcg ccgtctcttc tatcagttct ctgatgaatc aaaatattta cgatactttg
    20341 cacgcataag gaccatgcct cacaaacaaa tgcagtcata tgttaatatt gattatgaca
    20401 aaattttgtc gatagttggt cttattgagc atgccggtac tgagcgtatt attgctgaag
    20461 cacgatatgc ctatgatgaa aatgacaaat cgtatgaaat ggcatttatt gtggatgagg
    20521 aatttcaggg gaaaggtatt gctacatttt tagtaaggta cctcatgaag attgcaaaag
    20581 agcgtaagat agaacgagta actgcaaacg tattgcccca gaatgataag atgataaagg
    20641 tttttgaaaa atgtaatgtt ccctatacac agagatatga tgatggggtt atgcattttg
    20701 attttcattt aaaaaaagcg gagatagtac tttaaataca ataaatcaat atgttgctca
    20761 tgtcattttt gcagggaatc catcaataaa aaagcaaatt gcttccagtt atatagagcc
    20821 ctgaaagcca gataatgtga aaaggcatgc cctgttttat tagcaccacc agcattggtg
    20881 ttctcgccaa aaaaaccttt gcgattaaca tgaaaacttt tctgatcgcc ccaatcccat
    20941 gttgacactc catatattaa tgtaaatgct ggtaaagcaa taaacataac caatgtagga
    21001 gttgttggac tgttgccgtc agtaaagggc gcattgctga tctgaatatt gttgtcagat
    21061 gtataaacag aaggcgaaga tgagatgctc atccacggta aatgtcctta aaaatgagtg
    21121 tggaatggtt atggatattg aaaaaaaaat agtttattta acaatatttg agcgcgctgt
    21181 acaggtagct tcgtcgataa taaaatcacc aaaaacagaa ggcgcttctt tttgtaatat
    21241 tgcaagcatc tgtatgcaaa catttcttat ttcccattgg gcagcctttt caagccgcaa
    21301 tgaaaaaaca tggcgccatt ccctgaagtt tgccgaaatg acaatctggc tttccagtgc
    21361 attggggaga acaaagcgtg catcctcttt ttttatatgc atgcttctta actttttata
    21421 cgttttacga gcattatcca tgaattgcat atagagttct tttgattcgc cattatcagc
    21481 tattgatggt ggtataatat aattaaattc ttcttcatta acatacctct gtgattgctg
    21541 tgagaagctt gcaaggcggt gacgcaccat ctgatgggta aatgctcgcg atgctcccaa
    21601 tactcgaaaa gaggctgatg cgtgctccag tattgagtga tgtccattta tgatacatcg
    21661 acgtataaaa ttagcctcgc tgccttcggt tatcctatcc atggaaagat aacatgtcct
    21721 gcctgcctcc tctatgagtt tttcagcatt gggagttatt gccagaagag ttactgttga
    21781 tgaataaatt tccatagcta tctatcaaaa tttaatataa aaatacgcta aaaaacgtat
    21841 gcaaaacatg aaaaaaataa taaaaactat taaaagagct tcattccgga aaatccaaga
    21901 aatgaaatcg ccataagccc ggcggtaata aaggtaatag gcaatccttt gagattttca
    21961 ggtataaacg caagttcaag ccgctctcga attcctgcca taataataag cgctaaagta
    22021 aatcccacac caccaccaaa accctgtgtt actgaataaa gtaagccata ttgttgagta
    22081 atatatccag aatcaatatt caaaacagca acacctaaaa cagcacaatt ggttgttata
    22141 agtggtaaga agattcctaa tgcttgatat agtgacggtg aaatttttgc aattatcatt
    22201 tcaacaaact gaaccagcgt agcgataaca ataataaaag ctattgtacg gagatattca
    22261 acgtgataag gaagtaacaa taacttatat ataataaacg taaaaaaaga agccatggtc
    22321 ataacaaaga taactgcaag ccccatacct atagccgaat ccagtttttt tgatacacct
    22381 aaataagggc atagtcccaa tattcgtgac agaacatagt tattgataaa aattgtactt
    22441 atcaaaatgg tgaataatat tttaatttcc atatttgtcc ctcacttctg gttactcggt
    22501 tcgtttaaaa agtgcagcgc gtgccttaat gacattcatt ccccataaaa gtaaacctaa
    22561 tgtaaaaaat gcacctggtg ctaataccat taacagggca ggtttcatac cgggtatcaa
    22621 ttggtatcct aataatttat ttgcccctaa aatctcccga attgctgaca gaacagttaa
    22681 attcagagta aagcccaatc ccattccaat tgcatcaaga attgaatgta atatagtatt
    22741 ttttgaagca aactcttctg ctcgccctaa aattatgcaa ttaacaacta ttaatggaat
    22801 gaatatgcct aaagctttat ataccgatgg ttgatatgct tttaaagtca gttcaactat
    22861 aataacaaag cttgcgatta ttgtaataaa tattggaatc cgaacatgcg aaggaacata
    22921 ttctcgtatg gtagatatta atatattaga gcatagaaga acgaatgttg tagcaattcc
    22981 catccagata gcatttgaaa cagaagttga tactgcaagt gtgggacaca tgccaatacc
    23041 caatacaaat gtgggatttt tttcccatac tcctttgaat atttctttaa agtagttatt
    23101 cattgtttta cctcgtgcag agttaatgct tttttaagct tttgcgcacc tttttcaata
    23161 ctcagggtta ctgctttggt agtaatagtt gcaccggtaa ttgctgttat agcgttttgc
    23221 tgtaacagag catcttttat tgtattattc caatctcctt ttttcaaaat attgattggc
    23281 ttattggtgt caagcccctg aaattgaacc tggaaccatt ggatagcttc tgtgtttgtg
    23341 atgttgcttt taaaaaaatg atcaaaaaat gtttcatttg atgtgatttc agtacagcga
    23401 gcaccaagcc ccggagtttc actctgggac aaaattgtaa aacctaaaat ttttcctgta
    23461 tcatcaatcc caacaattga ttgaattgtg ccgccataac ctttttcttc agtaatgaat
    23521 gcatatgctt tttttattac gctgttttct tcctttgatg cttcccagta ggtaaaaggt
    23581 tgaccatcaa tagtaacagt gttttctttt tgaatagtat aggatggtaa tactaacgaa
    23641 agggaaagca attttttctc ttgatcttgc tttgctatgg gttcactggt aagcttattc
    23701 ataaacgaaa gcgaaaaagc agcaataaac cctataattg ttaataccaa tattgctttt
    23761 atcatttgaa aggtgttcat caatagttct ccactagtaa atgattatct atcataacta
    23821 tgtccttatc atgctccaaa tactttaggt ctggttaatt tatcaattaa gggtgtaatg
    23881 ccattcataa ttaatattga atatgaaacg ccttcaggat aaccacccca cagccgtata
    23941 attgaagcca gtattccaca gccaatacca aaaataatca tccctgtttt agtaacaggc
    24001 gaggtaacca tatcggttgc catgaaaaat gctcccagta taagccctcc tgataataca
    24061 tggaagagta tagcataatg gaggtttgga acacctgtaa agtaatagta tatccccata
    24121 caagcagcta cggttgcaat ataggatagc gggatatgcc atgtaataac tttcttccat
    24181 aatagtagca atgcacctaa caataaaagg atagccgatg tctcgcctat gcagcctcca
    24241 atattaccaa taaacaatga ctttatcatg gcgtttgaaa aaaggaaatc ataaagaggt
    24301 tgcgcctgta gcgatagttc ctgtaaaagt ttaggagctt cttttaatgc acccagcggt
    24361 gtagcgccac tgatactatc tattagttgc tggggcattg cagcattaaa gattggattt
    24421 tgcgcgatga cataaccatt gggaaatctg tgccacagtg ttgtcatctc caccggccat
    24481 gaagcaagta aaaatgctcg tcctgccagt gctggattaa atatgttgta tcccaatccg
    24541 ccaaatagct gtttgacaat gattatcgca aaagctgaac caatagcagg aatccatagc
    24601 ggcacatgtg gtggcaggtt cattgccaaa agtagtccag taataactgc cgaaccatca
    24661 ttgacagtaa ttggcctttt taaaagatat tgcattataa tttctgaaac tattgctgaa
    24721 attatggatg ttacaataac taatgcaaca tagagtccat ataaatagca tgaaaatatt
    24781 gttgcaggca taagcgaaag cactaccagc cacatcacaa gtggtactga ctgagtgtcc
    24841 ttgatgtgag ggggacttga taacactaaa tcttctaata ggcgtttttt atcagcatct
    24901 tttatttcca taggtgatcc ttctatagtg aaaatatgtt tcctcaatta tcgttttgca
    24961 ctcagtgtcg tttgtactac cttgataaag tgtgataacg gccgttttga cggacatata
    25021 tatgaacatg aaccacacat aatgcaatca aatggatgaa gcttttcggc aatatcatag
    25081 cgttgtttct cggctgcatt tcctaaatca cagggtataa gattcatagg acatactgaa
    25141 acacaacgtc cacatcggat acatgatctg aatggtgtaa ccttagtttc attttttgca
    25201 agaaaaagaa ttccggatgt tcccttaaca acaggtatat ccatgtggtt cagagcaagt
    25261 ccacacattg gcccgcccat aataatcttg gatggatttt caacaagtcc accgcattct
    25321 tccagaatat cactgacacg cataccaaat cgtactttat agttgccagg tttacgtaca
    25381 atttttcccg ttactgttat atatcgttct attaatggtt tatcaaaaag aactgcttcc
    25441 cgaatagcat aaatcgttcc cacattttgt acaacaacat ggacatccat aggtaacccg
    25501 cctgatggca cttcacgctt tgttattgta tagatgagct gtttttcagc accctgcgga
    25561 tatcgcttat ttagtggtat tataacaatc tcatgggttt ttacgtttgc tactgcagtt
    25621 tgtaatgctt taattgccag aggcttattt ttttcaatgc ctaaatatgc cttgtttata
    25681 cccaggattt tcattgcaat ctggatacct tcaataattt cttcagggtg ggtttgcata
    25741 agcatatcgt ctacagtaag gtatggctcg cattcagaac cattaataat taaggtgtca
    25801 atggttctgt tagctggtgg atttaattta actgctgtgg ggaatgcagc gcctcctaat
    25861 ccaacaatgc cagcagattg aactttt
//
LOCUS       DAOOJH010000028        24326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2133_length_24326_cov_10.042767, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000028 DAOOJH010000000
VERSION     DAOOJH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24326
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2133_length_24326_cov_10.042767"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            519..1667
                     /locus_tag="PLJ75_05605"
     CDS             519..1667
                     /locus_tag="PLJ75_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HPD04726.1"
                     /translation="MKSLSLLVKPVSYLCNLHCTYCFYKRVDALYPQTTIMSQEVLCR
                     LIISATNTGADFISFCWQGGEPALAGLDFFRAIVTLQNNYRKQHQVIENSIQTNGVLI
                     DEKWCSFLQENNFLVGISLDGPEEIHNNYRHTNAGSGTFEKVMQSITLMNEHRVHYNI
                     LTVLNDINIHHAKQVYRFFRENNFAYLQFIPCFEHDDNGALLPYSVTGLQLGRFYCDL
                     FNEWYRDGFPYVSIRLFQDLLMYSYEGVKASCCWLPCCDSYMVVEHNGDCYPCDFFVY
                     PQWKLGNIMSHSILDILGSPIRNAFSMVKQNMADECNECSIKDFCMGDCSRYRHGSTM
                     NAMNALNKSEYCTAWKVVYEHFQRSEGELQQRIRRLQKSLQSGSFFNT"
     gene            2360..3208
                     /locus_tag="PLJ75_05610"
     CDS             2360..3208
                     /locus_tag="PLJ75_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPD04727.1"
                     /translation="MIINHNMSAVNANRTLKFTQWSVNRSMEKLSSGERINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSFVQTAEGYLAQTAQIIQRIRVLAVQAANGIY
                     TNADRQLIQVEVSALVDEVDRIASQAEFNRFKILTGEFSKVNPKSSMWFHMGPNANQR
                     VRVFIGTMTAQAFRMKDATGKVAITLTSPGGANASIGIMDAALQKLSKQRADLGAYYN
                     RLEHTAKGLMTAYENVQASESRIRDTDMAEEMVDFTKNTVLVQSGTAMLAQANLQPQS
                     VLRLLV"
     gene            3518..4360
                     /locus_tag="PLJ75_05615"
     CDS             3518..4360
                     /locus_tag="PLJ75_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPD04728.1"
                     /translation="MRINHNLSAIFANRQLKIVSSRMDKSIERLASGQQINRAGDDAS
                     GLAVSEKMRTQINGLFQAGRNAENGLSFIQVAEGSLQQINDILQRIRMLSVQAAHGIY
                     SNSDRAQIQVEVSQLIDEIDRISTSAEFNRMKMLTGIYARNSRTGSIFFHVGPNQGQR
                     IRAYITTMSAKALNLYDGNRKRSISTVGQANAMIGYIDAALDKLNRQRADLGGYYNRM
                     ENTIYALAMSYENMLAADARIRDADMAAEMVEFTKDQILVQSGVAMLAQANFKPQLVL
                     HLLG"
     gene            4474..4803
                     /locus_tag="PLJ75_05620"
     CDS             4474..4803
                     /locus_tag="PLJ75_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HPD04729.1"
                     /translation="MDISKVTGNMSVNTFKVKQQEYKNEEKRLYEESKFTRENLDDAV
                     EMLNATAKVINNRIAFSVDEKTDRVVMRVVDVDNNEIIREIPPKEMIRLAAQIKDMIG
                     MFVDESR"
     gene            4914..6938
                     /gene="fliD"
                     /locus_tag="PLJ75_05625"
     CDS             4914..6938
                     /gene="fliD"
                     /locus_tag="PLJ75_05625"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HPD04730.1"
                     /translation="MPVTLGGVASGMDTDQIIQKLVEVEARPILQWQEEKDRYSLRKE
                     ALRQLQTKLTTLNTATKDLYGFRSPFKEKGVRISDPEVLQAQASRFAENGTHTLEVVN
                     LASTQKISTDQIKEDEILPPGTFSIEVDGNSYTIKFRGGNLKSLSDKINEIASSVVSA
                     TTIRTVDDKGILSIESKISGKKGELKLRGDEEYLKKIGLVKGEKGAEKEFQKIIFDTK
                     YFTAYTGEKPIEDEDGVLSVDKEGKSVIIDAKLWREYILPVEYQTKKQSVLEVNVTYS
                     KEKSEEEVIPYRLEVGPDETTVIKGIELKGYNVSRERPLEKKQKKEIADIIGVGVISV
                     EDGKRSERIYPIDANKQGKIEIPIGQDFAEKKITKILFYCNDGKAIFQDAALVTETKG
                     EGILEPKNIITQADDAKLKLNGIEIVRQKNSGIDDVLKGVTINLMKPSTKPLTITIKQ
                     NTEAAIEKIKKFVEAYNDYLTFNNELTKAVKSDKLGDYQKDKYKNGIFMGDMSLMRLQ
                     NALKTAVTEAYPSNVEKPVKMLYQMGVSTGEINAKWETIKEGKLIIDEAKLVEILSEN
                     PEGVQEFFGSDNDGDNRIDNGFAYSIEGILDPYVSAGKNIIVSKMDLEDASIKATNNR
                     IERKQEHLKTYEQKLRQKFASMEKAISGSKAQSNWLDMQMKNLPKDNDNK"
     gene            6952..7935
                     /locus_tag="PLJ75_05630"
     CDS             6952..7935
                     /locus_tag="PLJ75_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04731.1"
                     /translation="MQLLINDHPVDFAVEKETTVKDIMGSILNWASERSLVFSGAIVD
                     GTVYQLDNAPDISIEKVNNINCYVQSVSDIVIDSIKEGIAYCNRACQHLENKSNTSLS
                     ADELLQLQNGFEWIVSVLQSVLHMLGLERDDLRFKDMTVQEFIGSVYSFGHNISSSTD
                     INLLTTANNILHDTMTLFRVLLCSDALKSLVVQSVDSPDVLFQTMVDLKGMVGKQVKN
                     LEEIAGLYQKGNDSEALEIIHTLVDFLYLYMRTCYQVSPVFNINIAEFIYNGQSLEEL
                     NIKIQDMLTEIVSVMENNDVVSLTDILEYELKPLINEIDHYIDILLQNIKS"
     gene            8262..9653
                     /gene="argH"
                     /locus_tag="PLJ75_05635"
     CDS             8262..9653
                     /gene="argH"
                     /locus_tag="PLJ75_05635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPD04732.1"
                     /translation="MGKKKLWGGRFSNAPSKAAEEIATSLHYDMRLYKHDIRGSLAHA
                     KMLCKQKIISQKDCDDIQKGLSIIEQEIENGKFPFNETLEDIHMNIEARLTELIGDAG
                     KRLHTARSRNDQVITDVRLYLKDEMYEIKKLLIQLIVTIVQKAEETKHIVMPGYTHLQ
                     IAQPVRMAHHLLAYAWAMLRDVQRLEFAYVQTDELPLGSGALAGVNYTTDRQFLADEL
                     HFGAIAHNSMDAVSNRDFIADFLYFSSIMGMHCSRLAEDIILWSTAEFNFITLSDSVT
                     TGSSIMPQKRNPDVAELVRGKTGRLYGNLITLLTLLKGLPMTYNRDLQEDKEPLFDAI
                     DTVKLIASGMNEVITTMHVNGEVARKAIYKNFSTATDIADYLVMKGTPFRQAHEIVGS
                     IVKYCEQHNVDFFTLPLEALKKFSEQFDNDIYDYINPEKSTERKQSYGSTSLKSVEHQ
                     IMVIREKLHECGY"
     gene            9637..10341
                     /locus_tag="PLJ75_05640"
     CDS             9637..10341
                     /locus_tag="PLJ75_05640"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HPD04733.1"
                     /translation="MNVVIDSVTGLDIVHPFTVSMRILCALVAGFIIGFERERNYQPA
                     GLRTHMVLSLGACTVMILSYFIPYAYSPQNADPARLSAQVISGIGFLGAGAIFKYGFN
                     IKGLTTAATIWTTSGIGMVFGAGFYFLGVIATLFLVITLQLFEKVEMWLVEQKKTRIL
                     QVTYYSDAISGKEILTAIKKHTGDIDVRQVSVTEDVENKTSDFVVNCRIDEDFSLRLL
                     FDEIKQLGHIKKIKID"
     gene            complement(10611..11309)
                     /gene="ispD"
                     /locus_tag="PLJ75_05645"
     CDS             complement(10611..11309)
                     /gene="ispD"
                     /locus_tag="PLJ75_05645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPD04734.1"
                     /translation="MKHYAIILAGGLGQRMDSAVPKQFLALNDKPIIIWAIETFYTSS
                     LFDAIIIAIHPDYHKTLQSLLCDYNIQTTVIITNGGKTRQQSCYNALCSLQCNDNDIV
                     LIHDAARPFITQTMIQNCINTTVQYGACGIYTPVKETIAMIKDDSVVEVLPRHAICAA
                     QTPQCFYYSLIMDAHKKALAHSINDATDDISLVLIAGHPVKAITGNYYHNIKITTTED
                     IAIAQAIINNNLYH"
     gene            complement(11306..11797)
                     /locus_tag="PLJ75_05650"
     CDS             complement(11306..11797)
                     /locus_tag="PLJ75_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPD04735.1"
                     /translation="MVQVLILYYSRSGNTHELAKAIAEGVKQVENAQAIVRSTSEVTK
                     DDFLNSHGIIAGSPVYFGTMAAELKHLFDEYVIIRRKMENKIGAAFATSSDPSGGKET
                     TLLSILQAMLIYGMIIVGDPMEATGHYGVSCTGSPDEKTKKNAAALGKRVASLAKRIW
                     EQQ"
     gene            12076..13233
                     /locus_tag="PLJ75_05655"
     CDS             12076..13233
                     /locus_tag="PLJ75_05655"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPD04736.1"
                     /translation="MSYQTMISQRANEFTSFIVMDVMAKAEELERKGEHVIHLEVGEP
                     DFQTPEVIEQAAIEALKKQKTKYTHALGLLELREAISEYYYNEYNVSISPDRILVTTG
                     TSPALLFVMLAMLNHGEDIIVSNPRYPCYQNFIKAAGGRVNEIVTYPEEGFQYRPEDI
                     NRAITTKTKAIMINSPCNPTGIVMDEHALKNIAQFQNQFIISDEIYHGLVYNNKAHSI
                     LEFTNKAFVINGFSKLYAMTGWRLGYCIFPPEFTMVMQRIHQNFMISANNFIQWAGIA
                     ALKYAKNDVKAMQTIYNERRQYMIQGLKRLGFIVHVEPTGAFYVFADARTFCSDSYSE
                     AFNIIETVKVGVTPGIDFGSAGEGFLRFSYATSIENIEEGLKRIEQYLKNR"
     gene            complement(13403..14572)
                     /locus_tag="PLJ75_05660"
     CDS             complement(13403..14572)
                     /locus_tag="PLJ75_05660"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD04737.1"
                     /translation="MHYCIIGNGVAGTEAAFAIRSQDSSSEITVVTQSAHYMYYRPRL
                     IEYLSGTINLENLYIYKPAVYDEKHIELLFTTNVLAIDKNKKTIQLHNGADMHYDKLC
                     IATGAYPAIPDITGNNLNGVFTLREIEDADAILSHIDNAANKDVVVVGGGLLGLETAH
                     SLCKRNCNVTVIEFYDRLLPKQLDHEGAAIIKQLLEQKGLQFIVSESIASINKNEHGL
                     TLHCTSGRTIDAGTVIFSMGIKARTDLAKNAGLTVNRGIIINNYLQTSDPYIFAAGDP
                     SEHNGICYGIWTASREQGKLAGLNMAGVNNEYNGTLLSVVLKITGIDLYSAGDFTRKD
                     CTVLSQRDTSRYVKFLYKEKTPVASIVIGDMNIIKLARNVMEGKTSIDEFKALFT"
     gene            complement(14957..15466)
                     /locus_tag="PLJ75_05665"
     CDS             complement(14957..15466)
                     /locus_tag="PLJ75_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04738.1"
                     /translation="MQSILSKIIVVLFLVSCIGCSYIADSIEAELTNRANFSIDAYYD
                     SGSVMVSWDTHPDYDSFAGYEIYMTRDANDEFSEMIVVGAYYDIGNSSYFQQDATLGN
                     PSTSHFIHSNLPSPGIYFYRVGVIEWDERDYNNDGEDEKKPDFPDESLYKSYTHIEKI
                     SGSAMVEIF"
     gene            complement(15468..16913)
                     /locus_tag="PLJ75_05670"
     CDS             complement(15468..16913)
                     /locus_tag="PLJ75_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04739.1"
                     /translation="MNTVHYLNNSMKLALIHRKTINTMTAVVSVMGGKLQAYKMIVIF
                     FISIHLILQLLSPLHAQQVYSASTLHNAASRSQTIQHIFYQRWDNMQNNFCYFDAYVN
                     VNWVFDFDVNAYNRGTGETEAVPMKLVRTFSSMLLALPVYTPRDVTTNDYQQSPQTDI
                     TNATGALPIKKSWKKNSVLLGLSVCGYHYGLTRTVEISRGSAGSEKASDYVFSQFFDD
                     IAALTLIIVPHITIHCGVIFNQQIEPNDDGTLDYNFDPSKTAKRLLTSLSLFNAITWE
                     STAASNQLEATDISVNITQFISIVKTTPQYMPAIIIGYKQFKKYNDEDYDPVWVKNEK
                     LLGDSSRDSATLYSYYATVRKNIDNFYMAASIQLQHVDQTLIDKRTLETIDIPVLQMW
                     SMKAGYDFLHKPGWDFFVWLGVTHWYDPAISFHSNHNNNNVTGWFAMMDIASPLFTFT
                     LKGSYNDSYELEKLVESVDKYIIEGSASFRL"
     gene            17372..19489
                     /gene="fusA"
                     /locus_tag="PLJ75_05675"
     CDS             17372..19489
                     /gene="fusA"
                     /locus_tag="PLJ75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD04740.1"
                     /translation="MMLKEYATENVRTVAIVGHGSTGKSTLFDAMLFVGGQIDKIGKP
                     DDGTLTSDYDDEEKSRKMSIRSALGFVELDDTKINIIDTPGMSDFVGEARAALQVAEA
                     AIVVVDAVDGVQIETEKVWRYLESKKIPRIIFINKMDKERANFNTILENLKSHFNAKF
                     APLCIPLGEADKHSGVVDLIEMKAFVPKPDGKFQVSDVPADLKKAVDDARTNVMELSA
                     EGDDALIEKYLEGEALTEEEMARGLKTLVAKALVFPVVCGSSLKAIGVKNLLNVIKNY
                     VPAWELNKKIEGINPNNKEEKVIITSHPDAPFAAVVWKTYIDQYAGRFNYCKVISGKL
                     LPETEVLNSTKNYKERINKIYTMIGNKTVDVSKVLPGDIGVVVKLDRTATLDTLCDPK
                     QAVILPIIQLPHPVFSYAVQAAKKGDEDKIGQIFSRITDENPTITYEYNPETHQTVLS
                     GMGEMQLDIILKSIKEKNKIDLITSEPRIAYRETITKKAEAQYKHKKQTGGHGQYGEV
                     FIRVAPLERGKGFEFIDSIVGGVIPRNFIPAVEKGLREGMERGVLARYPVVDISVELY
                     YGSYHDVDSSEMSFKIAAIQALRKGLEAAGPILLEPVMEVEIFVDKEYMGDILNDITS
                     RRGRVLGMGSADEDGNSPISVVKATVPLAEMLRYSIDLRAMTSGKATFEMRFSHYDPI
                     SGKIAEKVIEERKKEFAEEEANK"
     gene            19759..21003
                     /locus_tag="PLJ75_05680"
     CDS             19759..21003
                     /locus_tag="PLJ75_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HPD04741.1"
                     /translation="MKIIVFEDSRYNDFYPFTMTRPVWDLRCGLFTIWQRYCRFYSSD
                     TFYFYTRHYLRDTAAHNYPELVINSPAAIAKSETVVFINARIIYPELSHINNNTVYYH
                     GGIPVLAKLTNIAIPQFATTEDVQIFLQDFPHQHEYSGLCISSIWELVSKNTEIIKHD
                     FTYVKGNQTTKYDVTTIGDSSQLIIEDGVTIEPYVVVDCTEGPVYIKKGCSIKALSRI
                     EGPCAIDEHTTLLQAKVRAGCSIGPWCRIGGEVEESIFSGYDNKYHEGFIGHSYIGEW
                     VNLGALTTNSDLKNNYTTVMINRGDEKIDTGLLKVGCFIGDFTMTGIGTLINTGSTMG
                     PCCMIVQSSGLTPKFIPPFCWYIGGKLVDLPSMNRLFETCKTMMSRRNHDFVIHHQKL
                     LEKVYEQTKVFRKGIGRWIAPK"
     gene            20985..21866
                     /locus_tag="PLJ75_05685"
     CDS             20985..21866
                     /locus_tag="PLJ75_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPD04742.1"
                     /translation="MDRAEVRFDLIVLISFFMHIIIMISFLLLQMRSGVLSKSAADSL
                     FKGRDIIVNINQDDIKDVNKNTFLSDKTSKAKGYITKDKGDRWLNNSLDFKMQKGVRR
                     LGKGGIASDAQTKDKVMVSTENTEYTITIFKEAMGGIPEFEGDSDFTRIPDKYSITPK
                     NALFFSSDGFFSFNTLKFKPFEYFKNMKDKVAANWHPPLLANAVIYGYDPMTGSFAPG
                     RMRIMAVPTQDVKLYFTMDRSGNVLDIVLVDSLGNKAIDESCVDAIRLSRNFGKVPEE
                     IKGRIIVIPFLFRIVSQ"
     gene            complement(21917..24208)
                     /locus_tag="PLJ75_05690"
     CDS             complement(21917..24208)
                     /locus_tag="PLJ75_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HPD04743.1"
                     /translation="MKKLIKRLKIRINEIINFIIQIINQIYRGDEAFNWLLTKLVNTI
                     KIFIVATRKFIKDDCLTKSSSIAYTTIVSLIPTLAVLLTIYSIFSGMGDKKDELFNQI
                     TIFLVEHNIKINVDPFFQTISSLIDNAAKIGGVGAVVLAFSATAVLRTLEKSLNDIFK
                     ISKQRSMFLKVIYYWAALTLGPIMLVAATAVATQISNTLSAPHFYSIAHYNNKNYITG
                     SKATILFNYNDNMKFSKLPQEKIDFDNQHIYTYNENEKKFNAEEYRIELIEYQRTNFY
                     DIEFAGNKGFIVGDNGILLKSNDSGNSWTIEKWGAFTFNDIKMLDENTGFIAADNGYI
                     LKTVDGGKTWQTMEWENITSNFNAIDFYNDKGIIVGDRSYIITTFDKGKIWQMQQLPQ
                     GKYKKNFLNFNNVQIVDENTVIIIADDGFYLKGNKNFNLWTPNKFKDNNLYALYFFNS
                     KKGLMAGEKADIYLTENGGEQWSFKKLKGTRINKLSFNNNLLWAIGNNSLFMLSKDNG
                     KSWQGTEGTGISIYLLNFFAPFFFIWILFLMCYMILPNTKVPLKPAAIGASFTSAVWV
                     IFILLFIIYIKAFAKSTFAIYGALAAFPLFLLVVYSSAVIILYGAEISYVLMNPLSYK
                     FLNKTLKDKKSIHVYTAVALLHHIYKKFESGKGASTFHELLPIALNDVDLLDMMLELF
                     EKEKFIIRTGNNAIIPANASKNITIAKIIDTVYSISLEIPPIKQDKLKSYLFDLFKDM
                     KQSIDLIVKDRTLSDIINATEST"
BASE COUNT         7943 a         3947 c         4934 g         7502 t
ORIGIN      
        1 tgcccggcaa ttgtttttat tttttgcaca aattgaggat tatatggaaa agattctata
       61 agaaatccgc tcttatcttt tttaattgta atcatttttt accatatcat agtatgatat
      121 ataaaaaaat ttcagaaaat taagagttta cattttttta atatacattc gttaatgctg
      181 tcacttttat atcactatca catttttttg tcaactttaa tttcccattg aaactttatg
      241 ttaacccctt tttcatagcg tgctttcaag ttctctccgg gaggaatgag attaacatgt
      301 tattatcaaa atcaaaattt atgcttgtat tttttttttg atcatctata aaaaattgat
      361 aagcgcataa tgcttacgtt taccgtgctg taataaaaat tgtataatag ttaatttcat
      421 gaaattattt atacacctta atcataacta tcataatcac atctaaacaa tatgtgcagt
      481 agcaaagaaa ctcttaatag taaacagtta gtatcataat gaaatcactt tcattacttg
      541 ttaagccggt atcgtatctg tgcaaccttc attgtaccta ttgcttttac aagcgggttg
      601 atgcactata tcctcaaaca actataatgt cgcaggaagt gttgtgcagg ttaataattt
      661 cagcaactaa taccggtgcc gactttatct cgttttgctg gcagggtggt gagccagcat
      721 tagcaggact tgattttttc agggcgatag ttaccttaca gaataactat cgtaagcaac
      781 atcaggttat tgaaaattca attcagacta atggcgtctt gattgatgaa aagtggtgta
      841 gttttttgca ggagaataac tttctggtgg ggataagtct ggatggacca gaagaaatcc
      901 acaataacta tcgccatacc aatgctggca gtggaacttt tgaaaaggtg atgcagtcaa
      961 taacattgat gaatgagcac agggttcatt ataatattct gacggtactg aatgatataa
     1021 atattcacca tgcaaaacag gtatacaggt ttttccgtga aaacaatttt gcatatctac
     1081 agtttatacc atgttttgaa catgatgaca atggtgctct gctgccgtat agcgttacgg
     1141 gtttgcaact tggaaggttt tactgtgatc tttttaatga gtggtatcgg gatgggtttc
     1201 cgtatgtttc catcaggctt tttcaggatt tgcttatgta ttcgtatgaa ggggtaaaag
     1261 catcctgttg ctggctgcca tgctgtgatt cgtatatggt ggtggagcat aatggtgatt
     1321 gttatccgtg tgattttttt gtgtatccgc agtggaagtt aggtaatatc atgagccaca
     1381 gtatactgga tatattaggg agtcctattc ggaatgcatt cagcatggta aaacaaaaca
     1441 tggcagatga atgcaatgag tgttccataa aagatttttg catgggcgat tgttctcgct
     1501 acagacatgg cagtaccatg aatgcaatga atgctttaaa taaaagtgaa tattgcactg
     1561 catggaaagt ggtgtatgaa cactttcaac gctctgaagg tgaattgcaa caacgaatta
     1621 gaaggttgca aaaatctctc caatccggtt cgttctttaa tacatagcgc aataatacca
     1681 gcccatgtgg ttcggggata aaatttaaaa aatgctgtgg cagagcacca ctggctttgt
     1741 gatacaaata tattttttat gataatgtgc tgcatggtcg cgtttcattt gtttcagatg
     1801 agcaataaag gaaagtctca aaggaaaaaa ttctggatgg ctccggcgca tggagcctcg
     1861 ttttcgcatg taaaaaaaaa ataagaatta gtttctttaa gtcaaaggat agtgatacat
     1921 ggaagaggat aacaagagta ttgaaagaaa aagcattgaa accgggtatc gattcttttt
     1981 tttgaaaaaa aactaaaaaa taatgaaaat agttaaaaat agataaaaag ataacatatt
     2041 tataaataat aacaaataaa accgtatatt aatctataac aacatataat catattcatg
     2101 ttttctacct acggtgacga aaagtcgagc agggataagt gtcttctgac attgttcttt
     2161 gaaaacacct tttggggtag tggtttgata tcaacaatga aggtttgatc ctttgtggtt
     2221 ctggtcttgt atttgcccag atacaagcct ggaacatagc agcattacaa atgctgctaa
     2281 cacattacat tgcgctgtct gcccggtgct atgtaatata tggcagggaa gccaaatctt
     2341 atgtgcaaag gagtgtaaaa tgattatcaa ccacaacatg agtgctgtga atgcaaacag
     2401 aactctgaag ttcacgcaat ggagtgtgaa caggagcatg gagaagctgt cgtctggcga
     2461 acgtataaat agagctggcg atgatgcttc tggactggca gtgtctgaaa agatgcgcac
     2521 ccagatacag ggtttgaggc aggcagaacg gaataccgaa gatggtatgt cgtttgtgca
     2581 gactgcagaa gggtatctgg cacagactgc ccagataatt cagagaataa gggttctggc
     2641 ggttcaggct gccaatggca tctataccaa tgcagaccgt cagcttattc aggttgaagt
     2701 gtctgccctg gttgatgagg ttgaccgtat tgcatctcag gcagaattca acaggtttaa
     2761 aatattaact ggtgaattct ccaaagttaa tccaaagtca agtatgtggt tccacatggg
     2821 tcctaatgct aatcaacgcg tgagggtatt cattggaaca atgactgctc aggctttcag
     2881 gatgaaagat gcaaccggta aagttgctat taccttaacg tcaccaggtg gagcaaatgc
     2941 ttccattggt atcatggatg cagcattgca aaagctctca aaacagcgtg cagatttagg
     3001 tgcttattac aacaggcttg agcatactgc taaagggctt atgacagcct atgaaaatgt
     3061 gcaggcatca gaatcccgta tacgggatac tgatatggct gaggagatgg tggattttac
     3121 caagaatacg gtacttgtgc agtcaggcac tgcaatgctg gcacaggcta atttacaacc
     3181 ccaatcggtg ttacgattac ttgtgtagct tagtagacac gttatggttc tttgaaaaca
     3241 tttgtgatga ttctcactct ccctggactg ttttcccggg gctgtccttt caattcggct
     3301 gcttgttctg gcggccggtg atgaagggag ggcgtcaccg gctgccagag atatggtggc
     3361 cgatgatgtc tggatgatgg gcagtccggg caacatgggg agtaaacccc tggtctaaaa
     3421 atgaccatac atgttcatga tgaacatgtt taaaaacctg agatatctca acataaacag
     3481 tgggatatcc tgttaaagtt caagggaggg agagtctatg agaatcaatc acaacttaag
     3541 cgctatattt gctaacaggc agcttaagat cgtatcatcg cgaatggata aatccattga
     3601 gcggttagca tccggccagc agattaacag ggctggggat gatgcttcag ggcttgctgt
     3661 gtctgaaaag atgaggacac agattaatgg tttgttccag gctggacgaa atgctgaaaa
     3721 tgggctttcg tttattcagg ttgctgaagg gtctctccag cagattaatg atattctgca
     3781 aaggatcagg atgctttctg tacaggccgc acatggtatt tattcaaatt ccgaccgtgc
     3841 acaaattcag gttgaggttt cacaactgat agacgaaatc gacaggattt caacatcagc
     3901 agaattcaat cggatgaaga tgctaacagg catttatgca cgaaattcgc gaacaggaag
     3961 tatattcttc catgtaggac caaaccaggg acaaaggatt agagcttata ttactactat
     4021 gagtgccaaa gctttaaacc tttatgatgg taatcgaaag cgttcaattt ccactgttgg
     4081 tcaggcaaac gccatgatag ggtatatcga tgcagcactg gataagctga atcgacaaag
     4141 ggcagacctt ggtggctact acaacaggat ggaaaatacc atctatgctc tggcaatgtc
     4201 ctatgaaaat atgttagctg ccgatgcccg tatcagggat gcggatatgg ctgctgagat
     4261 ggttgagttt acaaaggacc agatccttgt ccagagtggt gttgcgatgc tggctcaggc
     4321 taattttaag ccacagttag tattgcattt attgggatga gggtattacc cggttttcgc
     4381 agtgccagcc ggattttgcc ctctccataa aagaggagag ggcaacaatt tttcacaagg
     4441 atgttatcat gtacctacaa ggaggtatta actatggata taagtaaagt tacaggtaat
     4501 atgtctgtta acacctttaa ggtgaagcag caagagtata aaaatgaaga gaaaaggcta
     4561 tacgaagaat ctaaatttac acgggaaaat ttagatgatg ctgtggaaat gctcaatgct
     4621 acagcaaaag ttataaataa ccgtatagcc ttttcggtgg atgaaaaaac cgacagagta
     4681 gttatgcgtg tagtagatgt agataacaac gaaataatca gagagattcc acccaaggaa
     4741 atgatcagat tagctgctca gattaaagat atgataggca tgtttgtaga tgaatcaaga
     4801 tagtattacc ataatatgtc actttagtga ttgatgattt ttgccgataa tgtattgaaa
     4861 tagtatgggt atggatatat ctttactatt atcttacatg aagggataca tgtatgccag
     4921 tcactttagg cggcgtagca tcagggatgg atactgacca gattattcaa aagctggttg
     4981 aggtagaagc aaggccaata ctgcagtggc aggaagaaaa agaccgatac tccctccgta
     5041 aggaagcttt gcgccaattg caaaccaagc ttaccacctt gaatactgca accaaagatt
     5101 tatatggatt tagatcccct tttaaggaaa agggtgtacg catttcggat cctgaggtgc
     5161 tgcaggcaca ggcttcacgg tttgctgaaa atggaactca tacgctggaa gttgttaatt
     5221 tagcatcaac ccagaaaata tcaactgacc agattaaaga agatgaaatc ttgccacccg
     5281 ggactttcag tatagaagtt gatggtaatt catatactat caaattccgt ggtggtaatt
     5341 taaaatcgtt gagtgataag ataaatgaaa ttgcttcaag tgttgtatcg gcaacaacaa
     5401 tcagaacggt tgatgataaa ggtattttaa gcattgaatc taagatatca gggaaaaaag
     5461 gtgagttaaa actaagaggt gacgaagagt atttaaaaaa gataggattg gtaaaaggcg
     5521 aaaaaggtgc agaaaaagaa tttcaaaaaa ttatttttga taccaagtat tttacggctt
     5581 atactggcga aaaaccaatt gaagatgaag atggcgtttt atcggttgat aaagaaggta
     5641 aatcagttat tattgatgct aaattgtggc gcgagtacat ccttcctgtt gaatatcaga
     5701 caaaaaaaca atctgtatta gaagtcaatg taacctattc aaaagaaaaa tcagaagaag
     5761 aagttatacc gtaccggtta gaagttgggc ctgatgaaac tactgttatt aaagggatag
     5821 aacttaaagg atataatgta tcacgcgaaa gaccgcttga gaaaaaacaa aaaaaagaaa
     5881 ttgccgatat aataggtgta ggagttattt ccgttgaaga tgggaaacgc tctgagagga
     5941 tatatccaat tgatgcaaac aaacagggta aaattgaaat accaatagga caggacttcg
     6001 cagaaaaaaa aataacaaag atactatttt attgcaatga tggcaaggca atttttcagg
     6061 atgcagcatt agttactgaa acaaaaggtg aaggtattct tgagccaaaa aatatcatca
     6121 cacaggccga tgatgcaaaa ttaaaattaa atggcataga gatagtacgg caaaaaaatt
     6181 ccggcataga tgatgtgcta aaaggtgtaa caatcaattt aatgaaacca tcgaccaaac
     6241 cattaacaat aactatcaaa caaaataccg aagcagcaat tgaaaaaatt aaaaaatttg
     6301 tggaagcgta taatgattat ttaacattta acaatgaact gacaaaggct gttaaaagtg
     6361 ataaattagg cgattatcag aaggataaat ataaaaatgg aatttttatg ggtgatatgt
     6421 cgttaatgcg attgcaaaac gcgttgaaaa ctgcagtaac tgaagcatat ccatcaaatg
     6481 ttgaaaagcc tgttaagatg ttatatcaga tgggtgtttc tacaggtgaa attaatgcaa
     6541 aatgggaaac aattaaagaa ggtaaactca ttattgatga agcaaagctt gttgaaattt
     6601 tatcagagaa tcccgaaggt gttcaggaat tttttggttc ggataatgat ggggataatc
     6661 gtattgataa tggatttgct tacagcattg aaggtattct tgatccatat gtcagtgcag
     6721 ggaaaaatat tattgtttca aagatggatt tagaggatgc gtcaattaaa gctaccaata
     6781 atagaattga acggaagcag gagcatctaa aaacgtatga acaaaaatta cgacaaaaat
     6841 ttgcttctat ggaaaaagcc atatcgggat caaaagcaca aagtaactgg cttgatatgc
     6901 agatgaaaaa tctacccaaa gacaatgata ataagtgaca tgaggtacac tatgcaactt
     6961 ttaattaatg atcatcctgt tgattttgct gtagaaaaag aaaccacagt taaagatata
     7021 atgggttcaa tactcaattg ggcatcagaa cgaagccttg ttttttcggg agcgatagtt
     7081 gatgggacag tatatcaact ggataatgca ccggatattt ccatagaaaa agttaataat
     7141 ataaattgtt atgtacagtc ggtgagtgat attgttatcg actctataaa agaaggtatt
     7201 gcatactgta atagggcatg tcagcattta gaaaataaaa gcaatacatc attgtccgcc
     7261 gatgaattgc ttcagttgca aaatggtttt gaatggattg tatcggtatt acaatcggtg
     7321 ttgcacatgc tgggtttaga aagggatgat ttacgattta aggatatgac ggtgcaggaa
     7381 ttcattgggt cagtatattc atttggacat aacatttcca gtagcaccga tataaatttg
     7441 cttaccacag ctaacaatat tttacatgat accatgacgc ttttcagagt tttgctatgc
     7501 agtgatgctt taaaaagtct ggtagtacag agtgttgatt ccccggatgt actttttcaa
     7561 acaatggttg accttaaagg aatggtagga aagcaggtta aaaatttaga agaaattgcc
     7621 ggattatatc aaaaaggcaa tgatagcgaa gcacttgaaa ttatccatac acttgttgat
     7681 tttttatatc tttacatgcg aacatgttat caggtttcac cggtatttaa tatcaatatt
     7741 gccgaattta tatataatgg acaatcgctg gaagagctta atataaaaat tcaggacatg
     7801 cttactgaga ttgtctccgt tatggaaaac aatgatgtgg tgagccttac tgatatactg
     7861 gaatatgagt tgaaaccact gattaacgaa attgatcatt acatcgatat tctattgcaa
     7921 aatataaaat cataaaggct acccctatat gggtgcacta ttatagcaat gaataatccc
     7981 aattattaaa accctggtat gggtgatagt atactataaa aattgtaagt taacgagcag
     8041 attttgaaat caaacctggt atggcactga gatagtatgt tattctggaa acatggcatg
     8101 tcattctgaa caaagtgaag aatctcggtg ttttatgaaa cgaatagatt cttaaaaact
     8161 acgttcctca gaatgacaaa atggtggaga aattctttgg ttgcttttct attcatagta
     8221 atattgatga ataattttat ctttctttga ggagtaatgc gttgggaaaa aagaaattat
     8281 ggggcgggcg tttttctaat gcaccatcaa aagcagcaga ggaaattgca acatcgctcc
     8341 actatgatat gagattatat aaacatgaca ttcgtggctc attagcccat gcaaaaatgt
     8401 tatgcaagca aaaaattata tcacaaaaag attgtgatga catacaaaag gggctttcca
     8461 tcattgaaca ggagattgaa aacggaaaat tcccattcaa tgaaacgctt gaagatatac
     8521 acatgaatat tgaagcacgc ctgactgagc tcattggtga tgcgggaaaa cgtttgcata
     8581 cggcacgctc acggaatgac caggtcatca ctgatgtgcg gttatatctt aaagatgaga
     8641 tgtatgaaat aaaaaaactg ttaattcaat taatagttac tatcgtacaa aaagcagaag
     8701 aaacaaaaca tattgttatg ccgggatata cacatttgca gattgcacaa cctgtcagga
     8761 tggctcatca tctgctggca tatgcatggg caatgcttcg cgatgtacag cggcttgaat
     8821 ttgcttatgt gcaaacggat gaacttcctc tggggagcgg agcgctggca ggggttaatt
     8881 atactactga ccgacagttc ctggcagatg agcttcattt cggagctatc gctcataatt
     8941 caatggatgc agtaagcaat cgtgatttta ttgctgattt tttatatttt tcatcaataa
     9001 tggggatgca ttgttcacgg ttagcggaag acatcatttt gtggtcaact gctgagttta
     9061 attttataac attatctgac agcgttacta ccggctcttc aataatgcct cagaaacgca
     9121 atcctgatgt tgctgagctg gttcgtggaa aaacaggcag gttgtatggc aatttgatta
     9181 ctctgcttac gctactaaag ggtttgccca tgacctataa ccgcgatttg caggaggaca
     9241 aggagccatt gtttgatgcc attgatacgg taaagcttat tgccagcggt atgaatgagg
     9301 tgataaccac catgcatgtt aatggagaag tagcacgtaa ggcaatctat aaaaattttt
     9361 ctacagctac cgatattgca gattatctgg tcatgaaagg cacacctttt agacaggcgc
     9421 atgagatagt aggttcaata gttaaatatt gtgaacaaca taatgtggat ttctttacat
     9481 tgccacttga agcattaaaa aagttttctg agcaatttga caatgatatt tatgattata
     9541 tcaatcctga aaaatcaaca gaacgaaagc aatcgtatgg cagtacctcg ctaaaaagtg
     9601 ttgaacatca aatcatggta atccgggaaa aactgcatga atgtggttat tgacagtgta
     9661 acaggattag atatagtaca cccatttaca gtttcaatga gaatactctg tgcactggtt
     9721 gcaggtttta ttattggctt tgaacgtgaa cgaaattatc agccggcagg attgcgcaca
     9781 catatggtat tgtcactggg cgcatgtact gtcatgatcc tgtcatattt tattccttat
     9841 gcttattcac cgcaaaacgc tgatcctgca cgactgtcgg ctcaggttat aagcggcatt
     9901 ggctttttag gtgcaggagc catttttaaa tatggattca acattaaggg gcttaccact
     9961 gccgctacaa tatggacaac atcaggcatt ggcatggttt ttggtgccgg tttttatttt
    10021 ttaggagtga tagccaccct gtttttggta ataacattac aactgtttga aaaggttgaa
    10081 atgtggcttg ttgagcaaaa aaagacaagg attttgcagg taacgtatta ttcagatgct
    10141 ataagtggca aagagatcct tacagctata aaaaaacata ccggtgatat agatgtgcgc
    10201 caggtttcgg taacagaaga tgttgaaaat aaaacgtcgg attttgtggt aaactgccga
    10261 atagatgaag attttagcct gcggttactg ttcgatgaga taaaacagtt aggacatatc
    10321 aaaaagataa aaatagatta aaatatacaa ttatacaatt aatccatagt ttgcaaataa
    10381 ctataattct tgctggtaat gtccttatag atagttacct tagaaaagat ttaatcgaat
    10441 gaggtaaata gggattttgt aattgttttc tgtaaatttc aaattgtact acagattatg
    10501 aatatcgtgg attcttccgg gtggactcat gatgttcatt tcaaggattt acccaatgta
    10561 aactattttg aaaaaaagaa ataagatttt atatactact gcatgctgta tcagtgataa
    10621 agattattgt taattattgc ctgtgctata gcaatatctt cagttgttgt gattttaatg
    10681 ttgtgataat aattaccggt gatagctttg acaggatgtc cggcaatcaa aaccaatgaa
    10741 atatcatcag tagcgtcatt aatagaatgt gcaagtgctt ttttatgagc atccatgatt
    10801 agagaataat aaaagcattg tggcgtttgt gctgcacaga ttgcatgtcg aggtaaaact
    10861 tctaccacgc tgtcatcttt tatcatggca atagtttcct tgaccggtgt gtagataccg
    10921 caggcgccgt attgcactgt agtattaatg cagttctgaa tcatggtttg tgtaataaaa
    10981 gggcgtgcag catcatgaat gagcacaata tcgttatcat tgcattgaag tgaacagagc
    11041 gcattatagc aggattgctg ccgggtttta cctccattgg ttattatgac agtggtttga
    11101 atattgtaat cgcacaaaag tgactggagt gttttgtgat agtcaggatg aatggctata
    11161 ataatagcat caaacagcga agatgtataa aaggtttcta ttgcccatat aataattggt
    11221 ttgtcattca gtgccaaaaa ttgttttggg acagcactat ccatccgctg tcccagtccc
    11281 ccggctaata taattgcata gtgtttcatt gttgttccca gatgcgtttt gccagtgatg
    11341 caacccgctt tcccagtgct gcagcattct tttttgtctt ctcgtcaggc gaaccagtgc
    11401 atgatactcc ataatgaccg gttgcttcca tgggatcgcc aactattatc atcccataga
    11461 tgagcattgc ctgcagtatt gaaagcagtg ttgtctcttt ccctccagat ggatcacttg
    11521 atgtagcaaa tgccgctcca atcttatttt ccatttttct tcggataatg acatattcat
    11581 caaaaagatg ttttaactcg gctgccattg ttccaaaata aacaggtgaa ccggctataa
    11641 taccatggga atttaaaaaa tcatctttgg taacctcgct ggttgaacgc actatagcct
    11701 gtgcattctc aacctgtttt actccttcgg caatggcttt tgcaagttca tgagtgtttc
    11761 ctgaacgtga gtaatacaat atcaaaacct gcaccatgtt atacctcgta taatactgta
    11821 atgaaataca attattggaa attaatttac tgtatatgat ttatactata taaataataa
    11881 tagtattttt aattttgtca acagttacct gtgttttttt tgcgatagtt actgtaaaat
    11941 ttagcttgtc agctactatt atagatgttt atatagcttt tcagtgtcat tacaaacact
    12001 atcacacctg caatgcaagt atgttttaca gtgtgtatgt gcacatacaa ttgttatcga
    12061 atgagaaaaa taactatgag ctatcaaaca atgatatcac aaagggcaaa tgaatttaca
    12121 tcctttatag taatggatgt gatggcaaag gcagaagaat tggagcgcaa aggcgaacat
    12181 gttatccatc ttgaggttgg tgagcctgat tttcaaacac cggaggtgat tgaacaggct
    12241 gcaattgaag cattaaaaaa acaaaaaaca aaatataccc atgcgctggg tttgctggaa
    12301 ttgcgtgagg caatcagtga atactattat aatgagtata atgtttctat ttcgcctgac
    12361 cggatactgg ttactaccgg aacgtcacct gctttacttt ttgtaatgtt agccatgctc
    12421 aatcatggtg aagatattat agtatcaaat ccgcgttatc cctgttatca aaattttatt
    12481 aaagcagccg gaggaagggt aaatgaaata gtcacttacc ctgaagaagg atttcaatac
    12541 aggcctgaag atattaacag ggctataaca acaaaaacaa aagctatcat gataaattcg
    12601 ccatgcaatc caacagggat tgtaatggat gagcatgcat tgaaaaatat tgcacagttt
    12661 caaaatcagt ttatcatatc cgatgagatt tatcacggac ttgtctataa caataaagca
    12721 cattcaatat tggagtttac caataaagcg tttgttatta atggattttc caagctgtat
    12781 gccatgacag gatggcgttt aggttactgt atatttcctc cagaatttac catggttatg
    12841 cagcgcatac atcaaaactt tatgatatcg gcaaacaact ttattcaatg ggcaggaata
    12901 gctgcattga agtatgctaa aaatgatgtt aaagccatgc aaactattta taatgagcga
    12961 aggcagtata tgatacaggg tttaaaaagg ctgggcttta tagtccatgt tgaaccaacg
    13021 ggcgcgtttt atgtttttgc tgatgctcgt acattttgca gtgattcata tagtgaagca
    13081 tttaacataa ttgaaacggt gaaagttggt gttactcccg gcattgattt tggtagtgca
    13141 ggcgagggct ttttacgttt ttcttatgca acatctatcg aaaatattga agaaggatta
    13201 aaaaggatag aacaatattt aaaaaacagg taatgctgat cattgatgct gacagtttat
    13261 ttttttaatt gctcaatata tttacagtaa ctatcgcata gaaaattttt aattatgaaa
    13321 tgacagcact gcggtgagat gtgattaaaa aaaattttta agcaggcgat acacgatata
    13381 ctatacaata tatatactgc ctctatgtaa acagtgcttt aaattcatct atgctggttt
    13441 tgccttccat aacattgcgg gcaagtttaa tgatgttcat atcgccaata acaatgctgg
    13501 caaccggagt tttttcttta tacaaaaatt ttacatatcg tgatgtatct ctttgtgaaa
    13561 gcactgtgca gtctttgcgg gtaaaatcac cagccgaata gagatcaata ccagtaattt
    13621 ttaatactac cgatagcagc gtgccgttat attcattgtt aacgccagcc atattgagcc
    13681 ctgctaattt accctgttcg cgtgatgcag tccatatccc atagcatatc ccgttatgct
    13741 cgctgggatc accagcagca aaaatatatg gatcggatgt ttgcagatag ttattgatga
    13801 taattccacg gtttacagta agccctgcat tcttagctaa atcagttctt gccttaatac
    13861 ccattgagaa tatgaccgtg cctgcatcaa tagttcttcc cgatgtacag tgcagtgtta
    13921 gcccgtgttc atttttattg attgaagcaa tcgattctga tacaataaat tgcaaacctt
    13981 tttgttccag taactgtttg ataatagcag caccttcatg gtccaactgt ttgggaagca
    14041 atctgtcata aaattcaatg acggtgacat tacagttgcg tttgcacagg ctatgtgctg
    14101 tttccaatcc taaaagtccg ccgccaacta caacaacatc tttatttgcg gcattatcaa
    14161 tatgtgatag tatagcatca gcatcttcaa tctcacggag ggtaaacaca ccgttaaggt
    14221 tgtttccggt aatatcaggt atagcagggt aagcgccggt tgcaatacac agcttatcat
    14281 agtgcatgtc agcgccgttg tgcaattgaa tagttttttt gtttttatcg atggctaaaa
    14341 cgttagtggt aaatagcaac tctatgtgtt tttcatcata taccgcaggc ttataaatgt
    14401 aaagattttc aaggttaata gttccggaca gatattcaat gaggcgcggg cgatagtaca
    14461 tataatgagc tgattgtgtg acaacagtta tttctgatga actgtcctgt gaacgtattg
    14521 caaatgcagc ttcagtacct gcaacgccat tgccaataat acagtagtgc atggtttacc
    14581 cctttcgtaa ctataggtga cattgtaaat aatattcgaa gatggttttc aaaggattct
    14641 atccctgcca aataatgaac atcaaacaat acatcttttt atcagaaaaa caatactata
    14701 gtgcaaaaat agaatattgt gtaatatttt caaccaaaaa atgatcatgg catgggtcaa
    14761 ttgatttcac aatataaaaa acaaaatcat ataccaaagc aaaaactatt gcacgagtac
    14821 tcgatgcctg gcatattgct gcccattgtt aatggaaatt atacaaaagg aaacaatgcc
    14881 ccctcgcctt gagaaggaga gggtcggggg tgaggtcaac aacatctatt attgaagata
    14941 ttatccatgg ctatgattaa aatatttcaa ccattgcact gccgcttatt ttttcaatgt
    15001 gtgtatatga tttatacaat gattcatcag gaaaatcagg ttttttttca tcttcaccgt
    15061 cgttgttgta gtcacgctca tcccattcaa ttacgccaac acggtaaaag taaataccag
    15121 gtgatgggag attggagtgt ataaaatgtg aagttgatgg attaccaagt gttgcatcct
    15181 gttgaaaata tgatgaattg ccaatatcat agtaagcacc aactacaatc atttcagaaa
    15241 actcatcatt tgcatctctg gtcatatata tctcatagcc tgcaaatgaa tcgtaatcag
    15301 gatgagtatc ccatgatacc atgactgaac cgctgtcata ataggcatcg atggaaaaat
    15361 ttgcgcggtt tgttagctca gcttctattg aatctgctat atacgagcaa ccaatgcagc
    15421 ttactaaaaa caatactact attatttttg atagtatgga ttgcatatta taacctgaat
    15481 gaagcgcttc cttcaatgat atatttatca actgactcta ccagtttttc aagttcgtag
    15541 ctgtcgttat agctaccttt aagagtgaat gtaaaaagcg gtgacgcaat atccatcatg
    15601 gcaaaccagc ctgtcacgtt gttgttatta tggttgctgt ggaagctaat agcaggatca
    15661 taccagtgcg ttacacccag ccatacaaag aaatcccatc cgggtttatg taaaaaatca
    15721 tagcctgctt tcatggacca catttgtagt actggtatgt ctattgtttc cagtgttcgc
    15781 ttatcaatga gggtctggtc aacatgctgt agctgaatgc ttgctgccat ataaaagttg
    15841 tcaatatttt tcctgacagt ggcatagtat gaatacagcg ttgcactgtc acgggaacta
    15901 tcgcccaaca atttttcatt ttttacccag accggatcat agtcctcgtc attatacttt
    15961 ttaaattgtt tataccctat gatgatagca ggcatgtact gtggtgttgt ttttacaatg
    16021 gaaataaatt gagtaatgtt tacactgata tcggttgctt ctaattgatt gctggctgct
    16081 gttgattccc atgttattgc attaaaaagg gataaactgg taagaaggcg ttttgcagtc
    16141 tttgatggat caaagttgta atccagcgtg ccatcatcat tgggttcaat ttgctgattg
    16201 aagataaccc cgcagtggat ggtgatatga ggaacaatga taagcgtcag ggcagcgatg
    16261 tcgtcaaaga attgtgaaaa gacataatca gaagcttttt cgctgcctgc acttccacga
    16321 ctaatttcaa cggttcgtgt taatccatag tgatagccac atacagaaag tcccagtaaa
    16381 actgaatttt ttttccagga ttttttaatg ggtaaagctc cagttgcatt ggttatatca
    16441 gtttgtggtg attgctggta atcgttggtt gtaacatcgc ggggtgtata cactggtaat
    16501 gccagcaaca tgctggaaaa ggttcggaca agtttcatgg ggaccgcttc ggtttcaccg
    16561 gtgccgcgat tatatgcgtt aacatcaaaa tcaaaaaccc agttaacatt gacatacgca
    16621 tcaaagtagc aaaaattatt ttgcatattg tcccagcgtt gatagaagat gtgctgtatt
    16681 gtctggctgc gggatgcagc attatgcaat gtagaagcac tgtatacctg ctgggcatgc
    16741 aggggcgata gtaactgtaa aatgagatgg atgcttataa aaaaaataac tatcatttta
    16801 taagcttgca gtttgccccc catgacagaa actaccgcag tcatagtgtt gattgtttta
    16861 cgatgtatca aagctaactt catactatta ttgagatagt gcactgtatt catgtaacta
    16921 aaatctttca atttatagag aacgcattgc ttcatctgaa agagcagttg aagaagtact
    16981 ctcgcttaaa ataaaaacct tactcgttca gaaacctccc aattttcatc tcctccttgt
    17041 cattgcaatg tggctattgt gtaaactatc attataggtt atatgaaata agttattgca
    17101 agtaaaatat tgatatatca ttttcgaaat atcaaaataa aaactttttt taaatttcaa
    17161 gatatttctt ttgtaacaaa gcaggttggc gactctacta caatagaata aaaagataaa
    17221 aaaataatag ttaattgcca ctttcacaat tttatgaaat aaaatacttt acaaaaaaca
    17281 tatgccgttt tacttttcgg caaagctttt atcgtttagt ccttgaaaag aaatatttta
    17341 ttttatttta gtgtatagta agctggagga tatgatgcta aaagaatatg ctaccgagaa
    17401 tgtacgtact gttgccatag taggtcatgg aagtactgga aaatctacat tatttgatgc
    17461 aatgttgttt gttggcggac aaattgataa aattggtaaa cccgacgatg gtacacttac
    17521 atcggattat gatgatgaag aaaaatctcg taaaatgtca atacgcagtg cattgggttt
    17581 tgtagagtta gatgatacaa agataaatat tattgatacg cccggaatgt cagattttgt
    17641 tggcgaagca cgtgcagcat tgcaggtagc cgaagcagct attgttgtgg ttgatgccgt
    17701 tgatggtgta cagatagaaa cagaaaaagt atggcgttac cttgaatcaa aaaaaattcc
    17761 tcgaattata tttattaata aaatggacaa agaacgtgca aacttcaata caatacttga
    17821 aaatcttaaa tcgcatttta atgctaagtt tgcaccgctt tgtattcctc ttggtgaagc
    17881 agataaacac agtggtgttg ttgatttaat tgaaatgaaa gcttttgtgc ctaaacctga
    17941 tggtaaattt caggtatctg atgtgccggc agatttaaaa aaagcagtag atgatgcacg
    18001 aaccaatgta atggaactat cagcagaagg tgatgatgca cttattgaaa agtatcttga
    18061 aggtgaagca ttaaccgaag aagaaatggc aagggggtta aaaactctgg tggcaaaagc
    18121 tctggtgttt ccggtagttt gtggttcttc gctcaaagct attggagtta aaaatcttct
    18181 taatgtaata aaaaattatg taccagcatg ggaattaaat aaaaaaattg aaggcatcaa
    18241 tccaaataat aaagaagaaa aagtaattat tacttcacat cccgatgctc cgtttgcagc
    18301 agtggtgtgg aaaacataca ttgatcagta tgcagggcgg tttaattatt gtaaagtgat
    18361 atccggtaag ttattgcctg aaactgaagt attgaatagt acaaaaaatt ataaagaacg
    18421 tattaataaa atttacacga tgattggcaa taaaactgtt gatgtgtcaa aggtgttgcc
    18481 gggagatata ggcgtggtgg taaaactgga tagaactgct acccttgata cattatgtga
    18541 tccaaaacag gcggttatat tgcctatcat acagttgcct catccagtgt tctcgtatgc
    18601 tgtacaggca gccaaaaagg gtgatgaaga taagattggt cagatatttt ccagaataac
    18661 tgatgaaaat ccaacaataa cctatgagta taaccctgaa acacatcaga ccgttttatc
    18721 ggggatgggc gagatgcagc ttgatattat attaaaatct atcaaagaaa agaataaaat
    18781 agatttaatt acttctgaac cacgtattgc ttaccgggaa acaatcacaa agaaagccga
    18841 agcacagtac aaacataaaa agcaaacggg tggacatggg cagtatggcg aggtatttat
    18901 ccgcgttgca cctttagagc ggggtaaagg ttttgaattt atagacagta ttgttggcgg
    18961 agtcattcca agaaatttta tacccgctgt agaaaaagga ttgcgtgagg gtatggaacg
    19021 aggtgtgtta gcacgatatc ctgttgttga tatatcagtt gaattatatt atggctcgta
    19081 ccatgatgtt gattcttctg aaatgtcatt taaaattgct gctatccagg cattaagaaa
    19141 aggacttgaa gcagccggac caattttact tgaaccagta atggaagtag agatatttgt
    19201 ggataaagaa tacatggggg atatccttaa tgatatcacc agccgcagag gcagggtcct
    19261 tggtatggga agtgctgatg aagatggtaa ttcgccaatc tcggtggtta aggctactgt
    19321 accattagct gaaatgttgc gatattccat tgatctgcga gcaatgacca gtggcaaagc
    19381 tacatttgaa atgcgctttt cgcactatga tcctatatcc ggaaaaattg ctgaaaaagt
    19441 aattgaagaa cgcaaaaaag agtttgctga ggaagaagca aacaaataaa gaatgattta
    19501 cataactatt ataatcaagc cactggtagc aggtggcttg ttttatattg acagtcatta
    19561 tcaattacac tatattgtaa ctacatgagt tatactatat gtatgtaatg tcaaaaagaa
    19621 cattccgtga catgcatgga aaagtaagta gttgttgtca tttaaaagta aagctgatag
    19681 tcattttaca ccaatggaac ctgtgctgat tgaaggtaaa gcaaagaatc tctttaagtc
    19741 gtcaagttga aggttaccat gaaaattata gtttttgaag attctcgcta taatgatttt
    19801 tatcccttta ccatgactcg tcctgtatgg gatttgcgat gtggcttatt tactatatgg
    19861 cagcgttatt gccgtttcta ttcatcggat acgttctatt tttatacacg gcactatcta
    19921 cgcgatactg ctgctcataa ttatcctgag cttgttatca acagcccggc tgctattgca
    19981 aaatctgaga cggtggtctt tatcaatgca aggattatat atccggaact atcgcacata
    20041 aacaataata ctgtttatta tcatggcggc atacctgttc tggctaagct tactaatata
    20101 gctatcccac agtttgccac aaccgaagat gtacaaatat ttttacagga ttttcctcat
    20161 caacatgaat acagtggatt atgtatctct tcaatatggg aattggtttc caaaaataca
    20221 gaaataataa aacatgattt tacatacgtt aaaggtaatc aaacaacaaa atatgatgta
    20281 acaactattg gcgatagttc tcaattaatt atagaagatg gtgtaacgat tgaaccgtat
    20341 gtagttgttg actgtactga aggtcctgtg tatattaaga aagggtgcag tattaaagca
    20401 ttgagtcgca ttgaaggtcc ctgtgcaatt gatgagcata caacgctatt gcaggcgaag
    20461 gtgcgagcag gatgcagcat tggcccatgg tgccgtattg gtggtgaggt tgaagaaagc
    20521 atttttagtg gatatgacaa taaatatcat gaaggtttta ttggacattc gtacataggt
    20581 gaatgggtaa atttaggtgc actgacaacc aatagcgatt taaaaaacaa ctataccacg
    20641 gtgatgataa acagggggga tgaaaaaata gatacgggtt tactcaaagt gggatgtttt
    20701 attggtgatt ttaccatgac aggaataggt actttaatca ataccggcag cacaatgggc
    20761 ccgtgctgta tgattgtaca gagcagtggg cttacaccaa agtttattcc accattttgc
    20821 tggtatattg gtggcaaatt ggttgattta ccgtctatga acaggctatt tgaaacatgc
    20881 aaaacaatga tgtcacggag aaatcacgat tttgttatcc accatcaaaa gctgttggaa
    20941 aaggtatatg agcaaacaaa agttttcagg aaggggattg gtagatggat cgcgccgaag
    21001 taagatttga tttaattgta ctgatatcat tttttatgca tataataata atgatatcgt
    21061 ttttactatt gcagatgcgt tcgggggtgc tttcaaaaag tgctgcagac tcgttgttta
    21121 aaggcaggga tattattgtc aatataaatc aagatgatat taaggatgta aataaaaata
    21181 catttttgtc agataaaaca tcaaaagcga aaggttacat tacaaaagat aaaggtgaca
    21241 gatggttaaa taattcatta gattttaaga tgcaaaaagg tgtgcgaagg ttagggaagg
    21301 gtggcattgc cagtgatgct cagactaaag ataaagttat ggtatcaact gaaaatacag
    21361 aatacactat aactattttt aaagaagcta tgggtggaat acctgagttt gaaggtgaca
    21421 gcgattttac aagaataccg gataaataca gtattacacc aaaaaatgca ttgttcttta
    21481 gtagtgatgg ttttttttca tttaatacac taaaatttaa accttttgaa tactttaaaa
    21541 atatgaaaga taaagtagcg gctaactggc atccgcccct tttagccaat gctgttattt
    21601 atggatatga ccccatgaca gggagttttg cccctggcag aatgcgtata atggcagtac
    21661 caacacagga tgtaaagcta tactttacta tggataggag tggcaatgtg ctggatattg
    21721 tactggttga ttcattagga aataaagcta ttgatgaatc atgtgtagat gctatacggt
    21781 tatcgcgcaa ttttggtaaa gtaccggaag aaataaaagg caggattata gtcatcccct
    21841 ttttatttag aatagtttca caataagtaa atattaaaaa tagtgcactt taaatgtaaa
    21901 cgtgcatctt atcgtatcat gtcgattctg ttgcattaat tatatctgat agtgttctat
    21961 ccttaacaat taaatcaata gattgtttca tgtctttaaa caggtcaaaa agatatgatt
    22021 taagcttatc ctgtttaatt ggtggaatct ctaatgaaat gctgtatact gtgtcaatga
    22081 tctttgcaat tgtaatattt ttgctggcat tggcgggaat tattgcatta ttgcctgttc
    22141 ggattataaa tttttctttt tcaaatagct caagcatcat atcaagtaaa tcaacatcat
    22201 tgagtgctat gggtaataat tcatgaaatg tactggctcc cttccctgat tcaaactttt
    22261 tatagatatg atgtagtaat gcaacagcag tataaacatg aatacttttt ttatctttta
    22321 aagttttatt taaaaattta tagcttaatg gattcatcaa aacatatgaa atttctgctc
    22381 catagagaat tataactgct gaagaatata caaccagtaa aaatagtgga aatgcagcca
    22441 gagcaccata gatagcaaat gtgctctttg caaaagcttt aatatagatt atgaaaagta
    22501 atatgaagat aacccagaca gcgctggtga atgatgcgcc tattgcagca ggttttaatg
    22561 gtacttttgt attgggtaaa atcatgtagc acattaaaaa taaaatccat ataaaaaaga
    22621 acggagcaaa gaagttaagt aaataaatgg agatgccagt cccttcagtt ccctgccatg
    22681 attttccatt atcttttgat aacatgaata aactattgtt acctatagcc catagcaaat
    22741 tgttgttaaa tgataattta ttgattcttg tcccttttaa ttttttgaaa ctccattgtt
    22801 cgccaccatt ttcggttaaa tagatatcag ctttttcacc tgccattaat cctttttttg
    22861 aattgaaaaa atataaagca tataaattgt tatctttaaa tttatttggt gtccacaaat
    22921 taaaattttt attacccttt aaataaaatc catcatcagc aattataata acagtatttt
    22981 catctactat ctgaacatta ttgaaattaa ggaaattctt tttatattta ccctgcggta
    23041 attgttgcat ttgccatatt ttccccttat caaaagttgt tataatatag gatctatcgc
    23101 caacaataat tcctttgtcg ttataaaagt ctattgcatt aaaatttgat gttatatttt
    23161 cccattccat ggtttgccat gttttaccgc catctacagt ctttagtata taaccgttat
    23221 ctgctgcaat gaatcctgta ttttcatcta acattttgat atcattaaag gtaaaagcac
    23281 cccatttttc aatagtccat gaattaccgg aatcatttga ttttagtaaa atcccgttat
    23341 cacctacgat aaaacctttg ttgccagcaa attcaatatc ataaaaatta gtacgctggt
    23401 attctattaa ctctattctg tattcttctg cattaaactt tttttcattt tcattatatg
    23461 tataaatatg ttgattatca aaatcaattt tttcttgtgg caattttgaa aatttcatgt
    23521 tgtcgttata attaaaaaga attgttgctt tgctaccggt aatatagttc ttattattat
    23581 aatgagctat tgaataaaaa tgaggagccg aaagtgtatt ggaaatctgt gttgcgacag
    23641 cagttgcagc aacaagcatt ataggtccta atgttagagc agcccaatag tagattactt
    23701 tcaaaaacat tgatcgttgt tttgaaattt taaaaatatc attaagagat ttctcaagag
    23761 tacgcaaaac tgcagtagca gaaaaagcta aaacgacagc accaacacca ccaatttttg
    23821 ctgcattgtc aattaatgaa ctgattgttt gaaaaaaagg atcaacgtta attttaatat
    23881 tatgttcaac aagaaaaatt gtaatctgat tgaacagttc atccttttta tcacccattc
    23941 ctgaaaatat tgaatagatt gtaagcaata ctgccaacgt aggtataagt gaaacaatag
    24001 ttgtataagc aatagacgat gattttgtaa ggcagtcatc ttttatgaat ttacgggttg
    24061 caacaataaa tatttttatg gtgttaacta atttagtgag cagccagtta aaagcttcat
    24121 ctccacgata tatctgattt ataatctgga taataaaatt tataatctca ttgattctaa
    24181 tttttaatcg ttttattagc tttttcataa gaataatcct ttgatttgaa attcctgaac
    24241 agaaaattgt agtaaaaaat aatataagtc aaacataatt tatagtgcaa tatctaaaaa
    24301 atcaattttt gatattatta attgta
//
LOCUS       DAOOJH010000029        23079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2273_length_23079_cov_10.966122, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000029 DAOOJH010000000
VERSION     DAOOJH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23079
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2273_length_23079_cov_10.966122"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..818
                     /locus_tag="PLJ75_05695"
     CDS             318..818
                     /locus_tag="PLJ75_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04744.1"
                     /translation="MHISYLISSMTFMPRKHPAGFVAVVLLLPMLLVLAYCATDNSSA
                     RSLPGDITTAGFLSNDCYQCIVTAKPDAGVEGLTTSRESAHINLMKSIETKCYAELFA
                     AVLKLNPSAFGDSTMQSCAMQQLKPFVDKRILITTYFNEDYSETAVIRICAPGLKNSL
                     TTLHCK"
     gene            842..1282
                     /locus_tag="PLJ75_05700"
     CDS             842..1282
                     /locus_tag="PLJ75_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04745.1"
                     /translation="MIKVIKISSIVGCFLCCITIVFFSCAKKQIVQEPVPEQHNNLVL
                     EYEKEGFIDNNTFRVIMIVPAEEEYDELSVKQKGQERAFVSLKNYIMSRNKHYDSKAN
                     SYLMTTITEFGTLKKRESVSPSRYCYYFDITKSGLKKEIDALGQ"
     gene            complement(1283..1831)
                     /locus_tag="PLJ75_05705"
     CDS             complement(1283..1831)
                     /locus_tag="PLJ75_05705"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD04746.1"
                     /translation="MRKYIHYEKLSSEIKDAIYLYWGTLKQSNPALKFDEAMEDWFMH
                     QFDGFMIARYHIRGDNLRKHFRLDVEIPIRIVELLIESSKDEAEAMELIGTIVNISKG
                     GLYFISDMPLELSSIIRVVIDFGAVDDELSDVEALAMVVRHDTRDDKKYGIGVMFSSI
                     YDNGKHNLNIFILKNLSYYLYS"
     gene            complement(1908..2321)
                     /locus_tag="PLJ75_05710"
     CDS             complement(1908..2321)
                     /locus_tag="PLJ75_05710"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPD04747.1"
                     /translation="MSVTEIKVKGYHLDLFGRVTNQRFLDLLEDARWSYMDTIGLSFD
                     EFAKRGVFLAVVNINVNFRAPSILGDTLVIETSVDRIGNRSITVKQRMYNKKTEQIVL
                     DAEIVYVIVDLATGKSIPIDNGMRQIWLELSNKKN"
     gene            complement(2489..4141)
                     /locus_tag="PLJ75_05715"
     CDS             complement(2489..4141)
                     /locus_tag="PLJ75_05715"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPD04748.1"
                     /translation="MKKHLSIILLFTAIAVCGAYLEGMGAFKDMVKGLRSRELPSVYL
                     NFGRRGQIFKKMGLTYIVPLDNQYTDEGQSRITLDLSKLESRGEWIQAGKLPPENMKS
                     TTIINTKLVATGLPILSLYVDEHDLYDTFTGIYTNPLGRGRNWERPCFISYFNNGILL
                     FGTGAGVRIHGETSRLHAVKNFRIYLRDVYGKEYFGQNILFNGKGDPVRRFVIKKIES
                     DYGFTVAMAYAIARKMGCYASFADPVKFYLNGKPHGSGNFVLLEYLDTHYLQNHFGHK
                     NFVYYELKGKKDKPRQYQRLYEWAKFEGKSITFEKTSELIDMENFINYWIVNIFCANS
                     DPYQGVALLDTTKKNPRWFWLMWDMDHAFINIYEHDKKNLWEQERPINLVYTNTKKET
                     DPRYFIFRKLIFHDEQFRAVFKKRLSDALNYILTADYLASIVDKNSKVALSFGMDVKK
                     AHSELTEYMQKRPEYVMEMMHRYFKLGDVHTVRFTIPSGERVIIDGFEVTKSFTGYYF
                     NGAEISVSAINGKGLIINGKHNAPSTSFRITNDMTVQCLYMP"
     gene            complement(4158..4871)
                     /locus_tag="PLJ75_05720"
     CDS             complement(4158..4871)
                     /locus_tag="PLJ75_05720"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HPD04749.1"
                     /translation="MDISFQKIISILLKGFGSQKMEEIGFLPFLLIMLLSLLSAMLVS
                     YLYLKFYKNRATGSQIHRAFPLLGVSITAIFICIQFSLPLSLGLLGALSIVRFRTPIK
                     EPEEIGFIMVVVATSICCAVFNILFLGIVLLVTYIGLLILQRGPRFLKGRINDGMLII
                     TVPADDYTAKAGNIFNYLKQSLPLGKIESITENQDNTVISYSFNTIEDDMILKIKQEL
                     LSISNQISSNFFFNRIGDI"
     gene            complement(4902..5597)
                     /locus_tag="PLJ75_05725"
     CDS             complement(4902..5597)
                     /locus_tag="PLJ75_05725"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTC domain-containing protein"
                     /protein_id="HPD04750.1"
                     /translation="MITTNKVEHELKYIVSNSRAVDMIGWLSRYCEPDPEYPRGMISS
                     IYYDTLQWKYLDQKLNSFYLKTKVRLRWYSDLHYSCHDDAVFVELKYKIGSRRGKIRV
                     KSTYSGTEIAGMPLEDARLLMIPHILNNKGVIIKDKLLPAFQITYKRFRYIDPYSMSR
                     ICFDFDIHSPRVNSIMLPYMNPVMLQTAVFEVKGNESELPSSLYPLTDMGLKKSAFSK
                     YSACYRKLMRRHF"
     gene            complement(6022..7116)
                     /locus_tag="PLJ75_05730"
     CDS             complement(6022..7116)
                     /locus_tag="PLJ75_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04751.1"
                     /translation="MNIIQQMYASLCLVVVCSIVVLVAPVYGESIFLKDGSIVEGDIV
                     KETDKAMDVKLPDGNKITIPRKDIVRTLVNTNYKTKMYIMKTNKEVLPVYIVEEDNES
                     YTYRNELQSADEYKISKGDVLFLSKIPPQDWIADEVQKKVNELGVKKHYTWEQKVKWR
                     APLFRFGYSNIGKYADSDIDDAYSDSRDINAFIDLFPLRFRNESGSGFDVMMRVRAVG
                     NFDEPELYDEKTQLLSKLFDVTIGEEYEAQFNIGMIGGGIRYAYTLYYGVAIQPYVFG
                     LINYVVIDQIKVESTTETESFDEKISSSKYGFQAGVGVDIGLTPHIGIFVEGMYGYIS
                     AKFNDGKKHNIDGYYIYYGVTWRTSYGLID"
     gene            complement(7327..8307)
                     /locus_tag="PLJ75_05735"
     CDS             complement(7327..8307)
                     /locus_tag="PLJ75_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPD04752.1"
                     /translation="MLKRYYGALFITFVISTLYACKQQQNAVTARLIFMQGDVTIIQN
                     DINKLAGIGALVSPDDMITTGDKSIAVVQIADRAIAHIGSNSKVIVKSLLDTSVNLYL
                     EKGEVISKVERLQKGHEYMIKTRSVVASVRGTQFLVKSDEKIGKVAVNTGSVMVQPVI
                     ETEKGVVKESVIEGGKQAIVTVEEEKVTEEVPVTIQKISVKDKMKMEAMINLDVIPQD
                     VIMQPEAFEKVQETIKQNVEKMQEIESLSEDTIKQQIKKERIERLMQQKTRTLEEIKE
                     ACERIDVVKLYSGKEIQGAIISRGESYKILTTTGVVDVAKKDVRSISVIR"
     gene            8563..10938
                     /gene="lon"
                     /locus_tag="PLJ75_05740"
     CDS             8563..10938
                     /gene="lon"
                     /locus_tag="PLJ75_05740"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPD04753.1"
                     /translation="MPDDSQNIIESKHLILPSEILPEIIPIIPISHRPIFPGMMVPLV
                     LTGDTMLNTARAIAESDNKIGGVVLIQNQREGMVSSSDLYKVGVSIKILKIAPIDENT
                     IQVMVNALKRFTLMQVITEEPVIRWQVDHNYEPAAESTDEMKAYSAAILSSVKELIKS
                     NALFQEELKLFLNRFSLEDPGKLADFVASLTSAEPSEVQEVLETFDVKQRVEKVLLLL
                     KKELELSKLQKKISQQIEERIAKNQKEFFLREQLKEIKKELGLEKDEKTSEIEKFEER
                     VKKLRLSEEALKRFDEEIEKLKVLEPHSAEYGVSRNYLDWLTSLPWGVFSEDNYDIHK
                     AKKILDRDHYGLQDIKDRILEFISAGKKKGNITGSIICFVGPPGVGKTSIGKSVAAAL
                     NRKFYRFSLGGMRDEAEIKGHRRTYIGAMPGKIIQSLKVVETSNPVIMLDEIDKIGAS
                     FQGDPASALLEVLDPEQNSQFLDHYVDVRYDLSNVLFIATANQLDTIPLPLMDRMEIM
                     HLSGYILEEKLEIAKRYLIPKQLKEHGLLKNEVKIDTAALKKIIDGYAREAGVRSLEN
                     NIKKIMRKSTRQIIEGTAASIMVTPENLEEFLGKPRFTDESLYKKPIPGVVMGLAWTS
                     MGGATLYIEATAIPSKTKGFTQTGQLGNVMKESTEIAYSYIRSRVKQFGIDPDFFENH
                     FIHLHVPAGATPKDGPSAGITMATALYSLAKNKPIKKSVAMTGELTITGRVLPIGGVK
                     EKTIAAKRAAVKTIIYPIENKKDFDELPEHIKKGIDAHFVTYFEEVLDIVY"
     gene            complement(10945..11094)
                     /locus_tag="PLJ75_05745"
     CDS             complement(10945..11094)
                     /locus_tag="PLJ75_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04754.1"
                     /translation="MTLTAKDKKLLSQFRMQKAKDFLDDALDSYTSAVSIIQSIENVE
                     KDILL"
     assembly_gap    11124..11223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11501..11722)
                     /locus_tag="PLJ75_05750"
     CDS             complement(11501..11722)
                     /locus_tag="PLJ75_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04755.1"
                     /translation="MSTTNPAPYGVSIKSMVIAASLAKKRAISLSYSTGISFAITFVS
                     LVFSDSSRASPSRGPPHPIPDNTRRSAGC"
     gene            12208..13701
                     /locus_tag="PLJ75_05755"
     CDS             12208..13701
                     /locus_tag="PLJ75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HPD04756.1"
                     /translation="MYPTLEQCKKLSKHYNRIPIYKKLNIAEPGLLLLLKSFIEKGDV
                     IFLESAKQNKKQSRFSFLGFNPKATIVFTPSHIIIGDSYSQKIIHCTDPFRFIDDYIA
                     QYRSPSNPRFGLYNGGLSGYFAYDMVNYTGHLRQRVHEDDVHPLMVLHHIDDFICYDN
                     KYNIYYIATNIYTDTDLVDGAYNNAIEHLCKLEDDIIHVVQHTSLPYLPAYSDAIPVA
                     FQTSPEKFMDDVAKAKTLIEDGEALQIVLSMRALINEAVDPYRFYLKLRQVNPSPYMF
                     YMKYNDLHIAGSSPEIHVKVCNNTAMLRPIAGTIAQKKTKTGNAMQKTLLLHNEKERA
                     EHLMLVDLARNDLSIIAQPGSVRVTQFMQPEDYSHVIHLVSMVEALLHHDASLTDVLK
                     HTFPAGTVSGAPKVRAVEIINQIEDSPRGIYAGCVGYLGFNNTMDTCITIRTAVFSPQ
                     VSYMQAGAGIVYDSIPFNEYNEIVNKLQALAVSLPFAKVNSPVKEMA"
     gene            13701..14294
                     /locus_tag="PLJ75_05760"
     CDS             13701..14294
                     /locus_tag="PLJ75_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPD04757.1"
                     /translation="MFCIIDNYDSFTYNLVEYLRVLQQEVVIFKNDEPIDSIPLERYT
                     GIILSPGPSSPQNSGTTIAVIQQATTTPLLGVCLGMQAIAYVFGGTIVHAKTIMHGKT
                     DIIEHDGSKLFENIPRTFTAVRYHSLAVSSKDFPPCLSVCARSGDGEIMALSHNHRFI
                     WGVQFHPESYASEYGMQIVKNFIGGAYEYKRAYYTDN"
     gene            14260..15264
                     /gene="trpD"
                     /locus_tag="PLJ75_05765"
     CDS             14260..15264
                     /gene="trpD"
                     /locus_tag="PLJ75_05765"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPD04758.1"
                     /translation="MNTNELITQTIEGSTLSMDQSEYLFDAIFNGQISEISLAAVVAA
                     MKVRKEQPEEIAGAIKSMRKYAVSLPITEHVFDTCGTGGDHSRSFNISSAVAIILNAM
                     GYAIAKHGNRSVTSKSGSADFYEALNIPVHLSGEEATHYFNKTKFIFLFAPDYHPAMK
                     HAAPVRKQLGVRTIFNYLGPLTNPLQPKKQMIGVFHPSFLEVYSKAAIPLGYERLVLY
                     SSTNGMDEISPVHPTTVVEIQDNNSTTMVINPEDFITPQEASSIPYDKTPQENATLFI
                     DIIQSKKPQALSKLLALNTSLALYVLDHSSSIEKNYKDALEAIHSGCVASAVSKLQEV
                     "
     gene            15269..>15786
                     /locus_tag="PLJ75_05770"
     CDS             15269..>15786
                     /locus_tag="PLJ75_05770"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPD04759.1"
                     /translation="MQFSLALMKQKKLEEKQLIIALCKEHTMRKRPMYDWSFNSKIAI
                     IAEVKKASPSKGIIKIVDPAEQAVRYQQAGAKAISVLTDSHYFGGSFDDLCHVANAVT
                     LPVLCKEFICYTEQIDAAYLCGADSVLLINAMLTASELKTLYDYTIAKHLTPLIEIHA
                     LSELDTVLTLKP"
     assembly_gap    15787..15886
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15887..16123
                     /locus_tag="PLJ75_05775"
     CDS             <15887..16123
                     /locus_tag="PLJ75_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HPD04760.1"
                     /translation="GGTGKPFDWNSIAGFNQMGRTIIAGGLNSSTIPMLCSVVQPFGI
                     DISTGIEDKPGIKNINKMHAIIQTIKELGYDISR"
     gene            16107..17282
                     /gene="trpB"
                     /locus_tag="PLJ75_05780"
     CDS             16107..17282
                     /gene="trpB"
                     /locus_tag="PLJ75_05780"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPD04761.1"
                     /translation="MTYPDNNGYFGTFGGMFVPETLVNALTELEEAYNYFLHNDTMQN
                     QLNELLHHYAGRPTPLYFAQRLSKQTGIQLYFKREDLLHTGAHKLNNTLGQGLLTQFM
                     GKKRIIAETGAGQHGVATATVAALLGLECTIYMGQIDIERQMPNVKRMKLLGAEVIPV
                     TSGSQTLKDAINEALRDWVRNVKTTHYLLGSVTGPHPFPAIVRDFQKVIGSETKQQFQ
                     TLYGTLPDYCVACVGGGSNAAGFFYNFIGSTVKCIGVEAAGKGLFPGGHCASLTLGTP
                     GILHGSLSYLLQTSDGQIMDVYSISAGLDYPGVGPEHSFWKESNAVHYVTCTDEQALK
                     ACITLTQTEGIIPALESSHALGYVLCNSQKFNGSNVIINLSGRGDKDLDTILKEVKL"
     gene            17279..18037
                     /gene="trpA"
                     /locus_tag="PLJ75_05785"
     CDS             17279..18037
                     /gene="trpA"
                     /locus_tag="PLJ75_05785"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPD04762.1"
                     /translation="MKYNGFYLVGNYPDEETFLQAATIGLHYFDFMEVGLPFSDPIAD
                     GPVLASASHKALQNGATTDNVLKNITKIKDYIINNNCNKKIYIMTYANKVYHTTIDRT
                     IALFADSGVDGIILADVPYKESARFKQYTDSYHLDYIHFITPESTHTQIQQVCADAKG
                     FIYAISMRGTTGNTLTLDNTIIDILQLAKQYAKIPVVLGFGIQNNHDVQQALHYADGF
                     IIGTALEKKLEEGLDAYKQFIYQLHIQDKTKNIS"
     gene            18108..18500
                     /locus_tag="PLJ75_05790"
     CDS             18108..18500
                     /locus_tag="PLJ75_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04763.1"
                     /translation="MSNVKKAYLSILIYTIISIILSSIVYLSGGIPYGKGFAIGCLLS
                     LILTLLWIAGAIKGMKSNTLVLLSITAGGFLLRLTLLAVFAAGGVYIFMMDLPAFAIA
                     FLIGTIYSLVLEVWFFTTLSGPQNPKFR"
     gene            18511..20118
                     /locus_tag="PLJ75_05795"
     CDS             18511..20118
                     /locus_tag="PLJ75_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD04764.1"
                     /translation="MDTKTLQQLSQDELIKLIQQLQLKIDLLQKGVEEEPVDYKKLIE
                     AADDIIFVLDSEGNVVYRNRAMEDLFPVNSKDAVGMHFSLYLPAIELDRATMVFNQIL
                     YEGKSIHNEKMKTFDKDGNPVYLMANLAPIKDDDGKITGLFGILRNITEMHLMEKKLK
                     ESSRRLEEKIKEQLRQAEELKRLSTLNDEIINNSPIGIFTIDPTGIILSENPALRRII
                     GYGQDETRVGMNLTELPGFSVDLKKYLEEILLQKKPVFIKNLTYRPVDQDKELTLNVR
                     ANPILDFDKKVKSILIMIEDATEQAQIQKRMQRAEKLSAMGLLAAGVAYELKVPINLL
                     TIDLNFIENNIPETSPIHDYVKSMKDELSRINQITEQLLNLGKPGDDDVEVFEIHKLI
                     TSHPIQITLNRLQKSGFIINTAYPKESPKIKGRKNQLIQVLLHIISNAEEAMPDKGEI
                     RINVGSVIKNGEKFATITIEDTGIGIPSENLAKIFQPFFTTKGPKSTGLGLMVTYTII
                     NNHGGAIGVKSTPGEGTSFKILLPAVD"
     gene            20131..>21135
                     /locus_tag="PLJ75_05800"
     CDS             20131..>21135
                     /locus_tag="PLJ75_05800"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD04765.1"
                     /translation="MKSQNPSHRNYLSDAESLLDQEGYIIIAPDEDKQELQSEDKPTT
                     KTEHVVTQQVEKLPGKKPEIVTKTIEAEATDIPSTIQYLKWHMERIAKNVINREEIIK
                     QAMYAILTREHMLLLSRTGMAKSYLANYIFNTFDNVRVFASQASKEQTPDNYFGPYNI
                     EEFKKGRIRHNIHGSIIEANLVFLDEFFDASDVVLRSLLSVLNERKFINGPEIIDAAI
                     HTAIATANYMRMNEVTEAILDRFTYKSIIPEDDNVYQQLLIDHTYAFSRGKAIEPDKK
                     IPFNQIIYLSDIVRNKSKEVKIEIPDFVYFMKNVITNKFVSDMRKSDYKFFVSPRKQA
                     K"
     assembly_gap    21136..21235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21236..22609
                     /locus_tag="PLJ75_05805"
     CDS             <21236..22609
                     /locus_tag="PLJ75_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04766.1"
                     /translation="LSDIMVIDRYRTIYDLKKALPRELAWEDTIFDIKLFKHELLVQK
                     FYEEKADTFKPISTLQDESGRQANRFDQKFYLLLDRSRSMEIRMRSFFSKCLVAEFLR
                     RKLNSNAKIFYRPFDSKPGDLFKIEKKEDFPFLIERVLLTTTGGTSTNLQQAVFQAID
                     DINFDKDMLNSEILVVTDGISKIDKYLLKEKLGDIKLNILKVGRDLAEPDYFEMKKTF
                     DANKMKFDPTALNIKEIQQKMEKSQDGEEALSPLQKRAYRYILEYSETMFKDLKEISH
                     RFVEIPDLDPSSLFELTDEKLDAIETAVDDFTRIDYNTKTLEERKKLFKQAYFLSQYV
                     TLLLENKKNSSNPILKNCAYKLRKITQTILADPSLLQMVMETGRFTDKDTLKLSMKQL
                     KEKLKDMKMSQTPLSAEEIKKAQMLLTSDLGEGNIGQFLRVLFIKLLEFIKKLMQKIK
                     NAIKKNN"
     gene            complement(22606..>23079)
                     /locus_tag="PLJ75_05810"
     CDS             complement(22606..>23079)
                     /locus_tag="PLJ75_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD04767.1"
                     /translation="PGVDDAMILIILYMAMAMGDSNRLYIAGDGDSRYQLVHPDDVVE
                     ALLSIVRNPISIGKVYNIGSDNVPTQLEQVVKVKEIAQLDCQIKHITPLFTRILSILL
                     KPLHIHYLRKDHVQFILSNFVLDCNKIKEELKWIPKKDNIEIWVETLNWYRKEKL"
BASE COUNT         7416 a         4507 c         4123 g         6733 t
ORIGIN      
        1 accatccaaa agcagaccag atacgggata ttatagctat gttgaagaaa taatgataca
       61 taatattttt ttactttact attcagataa atcgaaatat caagctgttc taacggcata
      121 caaaattttc cctttacagc ttttttaaaa atcatattgt ttcttcatca ttaatttccc
      181 tttaattcta aatagtgctc cccatatttt ttaaaaagta taggtgaaaa taatttttta
      241 tttgacatat aacgatttgt tataatttag tatattttat tgactataat taacattttt
      301 aaactatgga tacgctcatg catatatcgt atttgatatc gtctatgacc tttatgccac
      361 gcaaacaccc tgcaggtttt gttgcagtag tattattgtt gcccatgctt cttgttttag
      421 catactgcgc tactgataat tcatctgcac gttcattgcc gggcgatata accacagcag
      481 gatttttaag caatgattgc taccagtgta tagtaacggc aaagcccgat gcaggtgttg
      541 aagggcttac caccagcagg gaaagcgctc acatcaacct tatgaaatca attgaaacaa
      601 aatgttatgc tgaacttttt gctgctgttt taaagctcaa tccttctgcc ttcggcgata
      661 gtaccatgca atcatgtgcc atgcaacaat taaaaccctt tgttgataaa cggatactga
      721 tcactaccta tttcaatgaa gattatagcg aaaccgctgt tatacggata tgtgcacccg
      781 gtttaaagaa ctcactaaca acattgcatt gtaaatgatg gcacaacaca agggagtaag
      841 tatgattaag gtaataaaga tatcatctat tgtaggttgt tttttatgct gcatcaccat
      901 agtctttttc tcatgtgcca aaaaacagat agtacaggaa ccagtacctg aacaacataa
      961 caatcttgta cttgaatacg aaaaagaagg ctttatcgac aataacacat ttagagttat
     1021 catgatagtc ccggctgagg aagaatatga tgagctatcg gttaaacaaa aagggcaaga
     1081 acgcgccttt gtttcgttga aaaattatat aatgtcacgg aacaaacatt atgattcaaa
     1141 agctaatagc tacctgatga caaccatcac agaatttgga acattaaaga aaagagaatc
     1201 cgtaagcccc agcagatatt gttattattt tgatattacc aaaagtggac taaaaaagga
     1261 aattgacgca ttagggcaat gatcatgaat aaagataata tgaaagattt ttcaatataa
     1321 agatattgag attgtgcttg ccattatcat agatgctgct aaacattact ccaataccat
     1381 attttttatc atcacgtgta tcatgtcgca ccaccattgc cagcgcttca acatcgctta
     1441 actcatcatc aacagctcca aaatctataa caaccctgat aatagatgac agttcaagag
     1501 gcatatccga aataaaataa agcccacctt tgctaatatt gacaatggta ccaataagct
     1561 ccatggcttc ggcttcatct tttgatgatt caattaaaag ctcaacaatg cgtatgggaa
     1621 tctcaacatc aagcctgaaa tgcttccgta ggttatcacc cctgatatga tatctggcaa
     1681 tcatgaaacc gtcaaactga tgcataaacc aatcttccat agcctcatca aacttaagcg
     1741 caggattact ttgctttaat gtgccccaat acaaatatat ggcatctttt atctcactgg
     1801 agagcttttc atagtggata tactttctca ttattccctc ttcactcata tattgactac
     1861 ccctttatca gggtacacta cccccataca taaagcaaat aaaattttta attttttttg
     1921 tttgatagtt ccagccatat ctgcctcata ccattatcaa taggtatgga tttacctgtt
     1981 gctaaatcta ctataacata aacaatctca gcatccagaa caatctgctc ggtttttttg
     2041 ttatacatcc tttgtttaac ggttatactc cggttaccga tacggtcaac cgatgtctca
     2101 atgacaaggg tatcgcccag tattgaagga gccctgaaat tgacattaat attaacaaca
     2161 gcaagaaaaa cacccctttt tgcaaattca tcaaaactca aacctatcgt gtccatgtaa
     2221 ctccatcgcg catcttccag aagatctaaa aagcgctgat tggtaacacg cccaaataaa
     2281 tccaggtgat atcctttaac ttttatctcg gttactgaca ttctgcacct tttttattgc
     2341 tatagtagta ctggttattg gtttcaatat acaaaaaaga ataatttttg caattagttt
     2401 tttgtttata tatttaaaaa tgtgcaatta gtagattgat ataaaccagt acaattaata
     2461 ccattttgat aacctaaaaa acccgtggct acggcatata tagacattgc actgtcatat
     2521 catttgttat gcggaatgat gttgaaggag cattatgctt tccatttata atcaatcctt
     2581 tcccattgat agcactcaca gagatttctg ctccattgaa atagtaaccg gtaaaacttt
     2641 tggtaacctc aaatccatca ataataacac gctcccctga agggatagtg aagcgtactg
     2701 tatgtacatc ccccagctta aaatatctat gcatcatttc cataacatat tctgggcgtt
     2761 tttgcatata ttcggtgagc tcgctatgag ctttttttac atccatacca aagctaaggg
     2821 ctactttact atttttatca actatcgatg caaggtaatc cgctgtaaga atatagttca
     2881 aggcatcgga cagccttttt ttaaagacag ccctgaattg ttcatcatgg aatataagct
     2941 ttcggaaaat aaaatatcgt ggatctgttt ccttctttgt gttggtataa accagattta
     3001 tagggcgttc ctgttcccac agattttttt tatcatgttc atagatattt ataaaagcat
     3061 ggtccatatc ccacataagc cagaaccagc gtggattttt ttttgttgta tcaaggagcg
     3121 caacaccctg ataggggtca ctgtttgcac aaaatatatt gacaatccag taattgataa
     3181 aattttccat atcaatcagc tctgaagttt tttcaaatgt aatagattta ccttcaaatt
     3241 ttgcccattc atagagacgc tgatattgac gcggtttatc tttttttccc ttcagctcat
     3301 aataaacaaa atttttatgc ccaaagtgat tctgaagata atgggtgtcc agatactcca
     3361 ataatacaaa atttcccgag ccatgcggtt tcccattcag ataaaatttt acaggatcag
     3421 caaacgatgc ataacaaccc attttccttg caattgcata tgccatggca acagtaaaac
     3481 catagtcgct ttctattttc tttatcacaa atcgtcggac aggatcacct ttgccattga
     3541 atagtatatt ttgtccaaaa tattctttac catagacatc tctgagatag atcctgaaat
     3601 ttttaacagc atggagcctg cttgtttcac catgaattct gacacctgca cccgtaccaa
     3661 aaagcagtat tccattgtta aaataggata taaagcatgg tctttcccag tttcgccctc
     3721 ttcccagtgg atttgtataa ataccggtaa aagtatcata aagatcatgt tcatccacgt
     3781 acagggaaag gataggtagc cctgtagcaa ctaatttcgt attgatgatg gttgtgctct
     3841 tcatattttc aggggggagt ttaccagctt gtatccactc tcctcgtgat tcaagctttg
     3901 acaaatctaa ggtgattcta ctttgacctt catcagtata ctgattatca agaggaacaa
     3961 tataggttag ccccatcttt ttaaatatct gcccacgcct cccaaagtta agatagacag
     4021 atgggagttc tctactccgc agacctttta ccatatcctt gaaggcaccc atcccttcaa
     4081 ggtaagcacc gcatacagca atcgctgtga aaagtaatat gatagacaga tgttttttca
     4141 tggcgcttct gatggtacta tatatcacct atcctgttga aaaaaaagtt tgaacttatc
     4201 tgatttgaaa tagataaaag ctcctgcttt attttcaaaa tcatatcatc ttcaatggta
     4261 ttaaagctgt atgatatcac cgtattatcc tgattctcgg ttatactttc aatcttaccc
     4321 agaggaagcg attgttttag atagttaaag atgtttcctg ctttagcggt atagtcatca
     4381 gccggaacgg ttatgataag catcccatca ttgatcctcc ctttaaggaa tcgtggacca
     4441 cgttgaagaa ttaaaagccc tatgtatgtt accagaagaa ctattccaag aaagaggata
     4501 ttgaaaaccg cacagcagat agaagtggca actacaacca taatgaaccc tatctcttca
     4561 ggctctttta taggagttct gaatcgcact atagaaagag cacccagcaa gcccaatgaa
     4621 agaggaagag aaaactgtat gcatataaaa atagcggtaa tagacacacc cagaagaggg
     4681 aaagctctat gaatttgact ccctgttgcc ctgtttttat aaaatttaag ataaagatac
     4741 gacaccagca ttgctgaaag caaagagagg agcataataa ggagaaaagg caaaaatccg
     4801 atctcttcca tcttctgact gccaaaaccc ttgagtaaaa tagaaataat tttttgaaat
     4861 gaaatatcca tagtacttat ccttaacggt aaaatttatt attagaagtg ccgtcgcatt
     4921 aatttcctgt agcaggcact atatttggaa aatgctgact ttttgagtcc catatccgtc
     4981 aaagggtaaa gcgatgaggg aagttcactt tcattccctt tgacctcaaa aaccgcagtc
     5041 tgaagcataa caggattcat gtatggcagc attatactgt ttactcgtgg cgaatgaata
     5101 tcaaaatcaa aacatatccg tgacatagaa taaggatcga tatatctgaa ccttttatag
     5161 gtaatctgaa atgcagggag cagtttatct ttgataatca cccctttatt gtttagtata
     5221 tggggtatca tcaacaaacg tgcatcctca agaggcattc ctgctatctc agttccactg
     5281 taagtggatt tgacgcgtat cttgccacgc cgcgaaccta tcttatactt caattcaaca
     5341 aatactgcat catcgtggca actgtaatgg agatcagaat accaccgtaa tctaaccttg
     5401 gttttaagat aaaagctgtt caatttttgg tcaagatatt tccactgcaa agtatcgtaa
     5461 taaatacttg agatcattcc ccgcggatat tcaggatcag gctcacaata ccgtgaaagc
     5521 catcctatca tatcaacagc acgtgaattg ctgacaatat atttaagctc atgttcaact
     5581 ttattggtgg ttatcatttt tcaatcaact atcgaacata taaaaagtat aaaactaaaa
     5641 aatcaatgca atacatacaa aaaatcgtaa ttactgtata ctcacagcag cccttttata
     5701 cctgtcaata aaaaaattca tagaaaacca aagtaatcag atgtctattt atacaggtta
     5761 aaagcgttga aatcatgatt aaatgattta atactattac catgaaagtg tgtactttta
     5821 cagctatacc attgatattt tactatttta ttttcatatt atgaatggtt agaattgata
     5881 ataaactatc tatacatagg aatgagttac atttataata ataaaatggc tccaataata
     5941 tttaattaac attattggag ccatagcaat gatataaaca aaaaatgtat tatccaagca
     6001 taagcattga taagtttaca attaatctat aagcccatac gatgttcgcc atgttacacc
     6061 atagtaaata tagtaaccat caatattatg ttttttacca tcattaaatt ttgcagagat
     6121 atacccgtac attccttcaa caaatatacc aatatgtgga gttaatccta tatcgacgcc
     6181 taccccagcc tgaaatccat attttgagga agatattttt tcatcaaaac tttctgtttc
     6241 cgttgttgat tctactttaa tttgatctat caccacataa tttattagtc caaatacata
     6301 cggttgtatc gccacgccat agtatagtgt gtatgcatat cgtatacctc cacctatcat
     6361 ccctatattg aattgtgctt catactcttc acctatagta acatcaaata gtttagataa
     6421 tagttgcgtt ttttcgtcat atagttcagg ttcatcaaaa ttacctacag ctcgtactct
     6481 catcatcaca tcaaatccgc taccactttc attcctgaac cgtaggggga atagatctat
     6541 aaaagcattg atatcacggc tatctgaata agcatcatcg atatcactat cagcatattt
     6601 tcctatatta gaatacccaa aacgaaataa tggagcacgc cattttacct tttgttccca
     6661 ggtgtaatgc ttttttacac ctaattcatt taccttcttt tgtacctcat ctgctatcca
     6721 atcttgtggt ggtatctttg aaagaaataa tacatctcct ttacttattt tatattcatc
     6781 cgctgattgc aactcattcc ggtaggtgta gctttcatta tcctcttcaa caatgtatac
     6841 cggcaaaacc tctttgttgg tcttcattat gtacatcttt gtcttataat ttgtgttaac
     6901 caatgttcgc acaatatctt tgcgaggaat tgttattttg ttaccatcgg gcaactttac
     6961 atccattgct ttatctgttt ccttgacaat atcaccttca acgatgctac catctttcaa
     7021 aaaaatagat tccccataca ccggtgctac aagtactact atgctgcata caaccaccaa
     7081 acataatgat gcatacattt gttgtattat attcataact cctcctaaaa taaatttatt
     7141 aacttcaccc ctccccttcg taaagcgagg agggcattgt ttgaggtttg tacaacgttc
     7201 atttcttatg ggtggctgca atacagtcat tggcaatccc acccaaaaac aaaaatatac
     7261 gatactaata aaccggcatc tccattgtgt aatggatatg ttgtttaaca tgtctattac
     7321 aataatttac ctgataacac ttattgatcg tacatctttc ttggcaacat caacaacccc
     7381 tgttgttgtt aaaatcttgt aactttcacc tctgcttata attgccccct gaatttcttt
     7441 acctgaatat aattttacta catctattcg ctcacatgcc tcttttattt cctctaaagt
     7501 tcttgttttc tgttgcatca atcgttctat acgctctttt tttatctgct gttttattgt
     7561 gtcttcggat aacgattcaa tctcttgcat tttttcaaca ttttgtttta ttgtttcctg
     7621 caccttctca aatgcttcag gttgcataat aacatcctgt ggtataacat caaggttaat
     7681 catagcctcc attttcattt tatcctttac cgatatcttt tgtatggtaa ccggaacttc
     7741 ttcagtaacc ttttcttcct caacagttac tatcgcctgc ttgcctcctt ctattaccga
     7801 ttctttgact actccctttt cagtttctat aacaggttgt accattacac tgccggtatt
     7861 aactgcaact ttgccaatct tttcatcgga ttttactaaa aactgtgtac cgcggacact
     7921 agcaaccact gaacgcgttt ttatcatata ctcatgccct ttttgcaggc gttcaacctt
     7981 ggatataacc tcaccttttt ccagatacag gtttaccgat gtatccagta atgattttac
     8041 aataacctta ctgttgctgc caatatgggc tattgcccta tcagcaattt gaaccactgc
     8101 tatggattta tcaccagttg taatcatatc atctggacta accaaagctc ctatacctgc
     8161 tagtttgttt atatcattct gaataattgt tacatcccct tgcataaaaa tgagacgtgc
     8221 tgtaacagca ttttgttgtt gcttacaggc atacaaagtg gatataacaa atgtaataaa
     8281 aagcgcacca taatatcgtt taagcatagc tacacctcga ttgataattt tttgatattt
     8341 aattagcagt gtaataatgt aataccagca tttattatat agtattatca acatataatt
     8401 gttaatctgt aaatcttttt tttaaaaata tttaaagacg tctggactaa aaataaccag
     8461 tgtgtaacca cttaccatat gcctgccaaa aaaaattatc ttgattttaa aaatctgcca
     8521 tgctacacta ctataaaaac aattcacaag aggtgatagc tcatgccaga cgattctcag
     8581 aatattattg aatcaaaaca tcttatactg ccatctgaga ttttacctga aattatacct
     8641 attatcccga tatcgcacag gccaatattt ccgggcatga tggtgccgct tgtactaacg
     8701 ggtgatacta tgctcaatac tgccagggca atagcagaat ccgacaacaa aataggcggt
     8761 gtggtattaa tacaaaacca gcgtgaaggg atggttagct cttcagatct gtataaggtt
     8821 ggtgttagca taaaaatttt aaagattgcg ccaattgatg aaaacaccat acaggtcatg
     8881 gtaaatgctc tgaaacgttt taccctgatg caggtaatca ctgaagagcc ggttatcagg
     8941 tggcaggtgg accataacta tgagccagcc gctgaatcta ccgatgaaat gaaggcatac
     9001 tcggcagcta tcctttcctc agtaaaagaa ttaataaaat cgaacgcttt atttcaagaa
     9061 gaacttaaac ttttcttaaa caggttcagt ctggaagatc caggcaaatt ggctgatttt
     9121 gttgcatcat taacctccgc cgagccatcc gaggttcagg aagtgctgga aacctttgat
     9181 gtgaaacagc gtgtggaaaa ggtgcttctc cttttaaaga aagagctgga gctttcaaag
     9241 ttgcaaaaaa agatttcaca gcagatcgaa gaacgtattg caaaaaatca aaaagaattc
     9301 tttttacgtg agcaattgaa agagataaaa aaagagcttg ggttagaaaa agatgaaaag
     9361 acatccgaga tagaaaaatt tgaagagcgt gttaaaaaac tgaggctcag cgaagaagct
     9421 cttaaacgtt ttgatgagga gatagaaaag cttaaagtgt tagaacctca ctcggcagag
     9481 tatggagtaa gccgtaatta tttagactgg cttacctcgt taccgtgggg tgttttttct
     9541 gaagataatt atgacatcca taaggcaaaa aaaatactgg acagagatca ttacggctta
     9601 caggatatta aagacagaat tcttgaattt atcagcgctg gcaagaaaaa gggtaatatt
     9661 accggttcaa taatttgttt tgtaggacct ccgggtgttg gtaaaacatc tataggtaaa
     9721 tccgttgctg ctgctttaaa ccgtaagttt tacaggtttt cgttaggtgg catgcgtgat
     9781 gaagctgaaa ttaaaggaca tagacgcacc tatataggtg caatgccagg aaagataata
     9841 cagagtctca aggtggttga gacatcaaat cctgttatta tgcttgatga aatagataaa
     9901 attggtgcaa gttttcaggg tgaccctgca tcagcactgc ttgaggtgct ggaccctgag
     9961 cagaattccc aatttttaga ccactatgta gatgtacggt atgatctctc caatgtactt
    10021 tttattgcaa ctgccaacca gttagatact attccactac cattgatgga tagaatggaa
    10081 ataatgcatc tttctggata tatactggaa gaaaaattag agattgcaaa gcgttattta
    10141 attccaaaac aattaaaaga acatggactt ttaaaaaacg aggttaaaat agatactgca
    10201 gcattaaaaa agatcattga tgggtacgcc cgtgaagcag gcgtacgcag tctggaaaat
    10261 aatataaaaa agattatgcg caaatctacc cgtcaaataa tagaagggac tgctgcatcc
    10321 attatggtga ctcctgaaaa tcttgaagaa tttttaggga aaccacgctt taccgatgaa
    10381 tcattgtaca aaaaaccaat cccgggcgtt gttatggggc ttgcctggac cagcatgggc
    10441 ggagcaacac tgtacattga agcaacggca atcccatcaa agacaaaggg atttacccag
    10501 acaggacagt tgggcaatgt tatgaaagag tcaaccgaga ttgcctattc atatattcgc
    10561 tcccgtgtta aacaatttgg cattgatcct gatttctttg aaaatcactt tattcatctc
    10621 catgttccgg caggagccac tcccaaggat ggtccttcgg caggcatcac catggctaca
    10681 gcgttgtatt cgcttgcaaa aaataaacca attaaaaaga gcgtagcaat gacaggcgag
    10741 cttactatca ccgggcgagt gctgcctatt ggaggagtta aagaaaaaac tatcgccgca
    10801 aagcgagcag ccgtaaaaac cattatttat ccaatagaga ataaaaagga ctttgacgaa
    10861 ttaccagagc atatcaaaaa agggatagac gcgcattttg ttacctattt tgaggaagtt
    10921 ctggatatag tgtattaaat acagctatag cagtatatcc ttttctacat tttcaataga
    10981 ctggattatt gatacggcac tggtatatga atccagtgca tcatctaaaa aatcttttgc
    11041 tttttgcatt ctgaattggc tcaaaagttt tttatctttg gctgtcaatg tcataacttg
    11101 ataccctcat tgataatatt ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnattgttg aggaattgcg aaaacaatag tatgaagaaa cttcatcccg actttataga
    11281 atttttgaga ttgatgaaaa aaaataaaat agctgctggc agggctaaag acattgccga
    11341 ttaagaatat ttgccgtaaa caataaaaaa attggaaata tataaaattt atagtatttt
    11401 aatattatct aaatatctta atatcaggaa taatgatgaa tataactatc accgacaacg
    11461 caaagagcaa aataatggat gctaccagag gggctgaatt tcaacaacca gcactgcgcc
    11521 ttgtgttatc agggattgga tgaggcggtc cccggctggg gctggctctg gatgagtcag
    11581 aaaataccaa tgatacaaaa gtaatagcaa atgatatacc ggtactgtat gataatgata
    11641 tcgctcgctt ttttgcaagc gatgctgcaa tcaccatcga ttttattgaa acaccgtacg
    11701 gtgccgggtt tgttgtcgac acaggagcat catgcagtta acgtatagtg caaaggagtt
    11761 tttttatgaa agaggcaatc tggtttttga tactgatggc attgtggttt gcattaaata
    11821 tctgggtttt tccaaaaatt ggcatacgga cctgactatc cagtagctgc cctgtggaac
    11881 agagcgctga taaaaatcaa cataatgata aaaaataaat tgacaaattt ttaaattgtg
    11941 attttctgtc actattataa ttattcattg caaagtcgaa gataatgtta agaaaatttg
    12001 aatatgtata gtagatcatt aacaataaac aataactggt ggtggtggac caatatgatg
    12061 tccacctgtt aggctattgt gcaatactat attttgaagc cgtgggtggg cattaaaccc
    12121 acggtttttt attgatccgg tatattccgt aagccgtggg tttaaaaaat ccacggcttt
    12181 tttattttac agcgtaagga gtttaccgtg tacccaacat tggagcagtg taaaaagtta
    12241 agcaagcact acaatcgcat accaatctat aaaaagctca acattgcaga gccaggattg
    12301 cttttgcttt taaaatcatt tattgaaaaa ggagatgtta tcttccttga gagtgcaaaa
    12361 caaaataaaa aacaaagcag gttttcattt ttaggattta atccaaaagc aactatcgtc
    12421 tttacgcctt ctcacattat tattggcgat agttactctc aaaaaataat acactgcact
    12481 gatcctttca gattcattga tgattatatt gcgcagtacc gctcaccatc caatccacgt
    12541 tttggcttat acaatggcgg gctttcagga tactttgcct atgatatggt aaattacacg
    12601 ggacatttac gccagcgtgt ccatgaggat gatgtccacc cccttatggt actgcaccat
    12661 attgatgatt ttatatgcta cgataacaag tacaatatct actacatagc tacaaacata
    12721 tatactgata ctgatctcgt tgatggtgca tacaacaatg caattgaaca tctttgcaag
    12781 cttgaagacg atatcatcca tgttgtacag catacatcac ttccctatct tcctgcatac
    12841 tcggacgcta ttccagtagc atttcaaacc tcgccggaaa agtttatgga tgatgttgca
    12901 aaagcaaaaa cgctcataga agatggcgag gcattgcaaa ttgtgctgag catgcgtgca
    12961 ctcatcaatg aggcggttga cccataccgc ttttacctga agctccgtca ggtaaatcca
    13021 tcaccgtata tgttctatat gaaatacaat gaccttcata ttgccggcag ctcaccggag
    13081 attcatgtca aggtgtgcaa caatactgca atgcttcgcc ccatagcagg aactatcgcc
    13141 cagaaaaaaa caaaaacggg aaatgccatg caaaaaacat tgctgctcca taacgaaaag
    13201 gaacgtgctg agcacctgat gctcgttgat ttagcacgca acgatctgag cattattgca
    13261 caacccggtt cggtgcgtgt tacccagttt atgcaacctg aagattattc gcatgtgatc
    13321 catttagtat caatggttga agcgctgcta catcatgacg cgagcctaac cgacgtttta
    13381 aaacatacat ttcccgccgg gacggtgagc ggcgcgccca aggtacgtgc cgttgaaata
    13441 attaaccaga ttgaagatag ccctcgcggc atctatgcag gatgtgttgg ctaccttggt
    13501 tttaacaaca ccatggatac ctgcattacc atccgaactg ctgtgttttc gcctcaggtg
    13561 tcgtacatgc aggctggtgc ggggattgtt tacgacagca taccgttcaa tgaatataac
    13621 gaaatcgtca ataaacttca agcgctggca gtaagtttgc catttgcaaa agtaaattca
    13681 ccggtgaagg agatggcatg atgttctgta tcatcgataa ctatgattcg tttacctata
    13741 atctggttga atatctgcgt gttttacagc aggaggttgt catttttaaa aatgatgaac
    13801 caatcgattc catccctctg gaacgctata ccggtatcat actttcgccg ggtccatcct
    13861 cgccgcaaaa ttccggcact accatcgcag tcattcaaca ggcaaccacc acaccgctat
    13921 tgggcgtatg tctggggatg caggctattg cctatgtttt tggcggtacg atagttcatg
    13981 ctaaaaccat catgcacggc aaaaccgaca tcatagaaca tgacggcagt aaactttttg
    14041 aaaacatccc caggacattt acagcagtac ggtatcattc gcttgcagtc agcagcaagg
    14101 attttccgcc atgcctttca gtatgtgccc gttcaggcga tggcgagatt atggcgcttt
    14161 cacataacca tagatttatc tggggtgtac agtttcatcc cgaatcatat gcatcagaat
    14221 acgggatgca gatagtaaaa aattttatag gaggagctta tgaatacaaa cgagcttatt
    14281 acacagacaa ttgaaggcag taccctgtcc atggaccagt cagagtatct ctttgatgct
    14341 atatttaatg gacagatttc agagatttca ttagccgcag tggttgcagc aatgaaggtg
    14401 cgcaaagaac aaccggagga aatagcagga gcaataaaat ccatgcgcaa atatgccgtg
    14461 agccttccca tcaccgagca tgtttttgat acatgcggta caggcggtga ccactcacga
    14521 tcgttcaaca tctccagtgc agttgcaatt atactcaatg ccatggggta tgcaattgca
    14581 aaacatggca accgatcagt aacaagcaaa agcggatcgg ctgatttcta tgaagcgctc
    14641 aatatccctg tccatttatc aggagaagaa gccacccatt atttcaataa aacaaaattc
    14701 atctttttat ttgcacctga ttatcatcct gcaatgaaac atgctgcccc ggtgcgcaaa
    14761 cagctgggag taagaaccat atttaattat ttagggccat tgaccaaccc gcttcaaccc
    14821 aaaaagcaga tgattggtgt attccatcct tcgtttctgg aggtttattc aaaagcagcc
    14881 attccgttag gttatgagcg gcttgttttg tattcttcaa ccaacgggat ggatgaaatc
    14941 tcaccggtgc acccaaccac ggttgttgag atacaggaca ataacagcac taccatggtt
    15001 atcaatccag aggattttat cacaccacag gaagccagca gcatacccta tgacaaaacg
    15061 cctcaggaaa atgcaacgct tttcattgat atcatacagt caaaaaaacc acaggcgtta
    15121 agcaagctgt tggcgctcaa tacatcatta gcgctgtatg ttcttgatca cagctcttca
    15181 attgaaaaaa attataagga tgcgctggaa gccatacaca gcggttgcgt tgcatccgcg
    15241 gttagcaaac tacaggaggt ttaatgcaat gcagttttca ctggcactca tgaagcaaaa
    15301 aaagctggaa gaaaaacagt tgattattgc actgtgtaaa gaacacacca tgcgaaaacg
    15361 ccccatgtat gattggagtt ttaacagtaa aattgctatt attgcagagg taaagaaagc
    15421 ttcgccatca aaggggatca ttaaaatagt agaccctgct gaacaggcag tgcgctatca
    15481 gcaggcagga gcaaaagcta tcagtgttct tacggatagc cattattttg gcggaagctt
    15541 tgatgacctg tgccatgttg ccaatgcggt aaccctgcct gtactttgca aagaatttat
    15601 atgctatacc gagcagattg atgcagcata tctatgcggt gccgacagtg ttcttttaat
    15661 caatgcgatg cttaccgctt cagaattaaa aacactgtat gattacacta ttgcaaaaca
    15721 cctgaccccc cttattgaaa tccatgcact cagcgaatta gataccgtgc ttacactgaa
    15781 accacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggag gaaccgggaa
    15901 accatttgac tggaatagca ttgccggatt taatcagatg ggaagaacca ttatcgcagg
    15961 agggctaaac agcagcacca tcccgatgct gtgctcggtg gtgcaaccgt ttggcattga
    16021 catctccacg ggcatagaag acaaacctgg tattaaaaat attaataaga tgcatgcaat
    16081 tatacaaaca atcaaggagt taggctatga catatcccga taataatggt tattttggaa
    16141 catttggcgg aatgtttgtg cccgaaactt tagtcaacgc acttacagag cttgaagaag
    16201 catacaacta ttttttgcat aatgatacaa tgcagaacca attaaacgag ttgcttcatc
    16261 actatgcagg caggccaaca ccgttgtact ttgctcaaag gctttcaaaa caaactggca
    16321 tacaattgta ttttaagcgt gaggatttac tccataccgg agctcacaaa ttaaacaata
    16381 cactgggtca gggattgctt acacaattta tggggaaaaa acgcattatt gccgaaacag
    16441 gtgccggtca gcatggtgtt gcaactgcaa cggttgcagc gcttttaggg cttgaatgca
    16501 ccatctatat gggacagatt gacatagagc gtcagatgcc caatgtcaag cgcatgaagc
    16561 ttttaggtgc cgaggttatc cccgtaacat caggaagcca gacgctgaaa gacgccatca
    16621 atgaagcact gcgcgattgg gtacgtaatg tgaaaaccac ccactatctg ttaggctcgg
    16681 ttaccggtcc acaccccttc ccagcaattg tcagggactt tcaaaaagtt ataggctctg
    16741 aaaccaaaca gcaattccag acgctctacg gaaccttacc tgattattgt gttgcctgcg
    16801 ttggcggcgg cagtaacgct gctggctttt tttataattt tattgggtcg acagttaagt
    16861 gtataggtgt tgaagctgca gggaaaggac tattccccgg cgggcattgt gcatcactga
    16921 ccctgggcac ccccggaatc ctccatggta gtttaagcta tctactgcaa accagcgatg
    16981 gacaaataat ggacgtttat tccatatctg ccgggcttga ttaccccgga gttggccccg
    17041 aacacagctt ctggaaagaa agcaatgcag tccactatgt tacctgtacc gacgaacagg
    17101 cgcttaaggc atgcatcaca ttaacacaaa cagaaggcat tatcccggca ttggaaagca
    17161 gccatgcatt gggctatgtt ttgtgtaata gtcaaaaatt caatggctca aatgtcataa
    17221 taaacctttc aggacgtggc gacaaagatc ttgataccat attaaaagag gtgaagctat
    17281 gaaatacaac ggtttttatc tggttggcaa ctaccccgat gaggaaacat ttttacaggc
    17341 tgcaaccata gggctgcact actttgattt tatggaagta ggactgccat ttagcgaccc
    17401 tattgccgac ggacccgtac tggcatcagc ctcccacaaa gcattgcaga atggcgctac
    17461 taccgataat gttttaaaaa atattacaaa gatcaaggat tacatcatca acaataattg
    17521 taataaaaaa atatacatta tgacctatgc aaataaggta taccatacta ctattgaccg
    17581 tactattgct ctgtttgcag atagtggcgt tgatggaata attttagctg acgtccccta
    17641 taaagaatct gcacgcttta aacaatatac tgatagctat catttagatt acatccattt
    17701 tattacacca gaatcaacac atactcaaat acaacaggta tgtgctgatg ctaagggttt
    17761 tatctatgcg atatccatgc gtggaactac tgggaatacc ctgacattgg acaatactat
    17821 aatcgacata ctgcaattag caaagcaata cgcaaagata ccggttgtac tgggatttgg
    17881 aatacagaac aaccatgacg tacagcaggc actacactat gctgatggtt ttatcatagg
    17941 aactgcactg gaaaaaaaat tagaggaagg attggacgca tataaacagt ttatctatca
    18001 attacacatt caggataaga caaaaaatat tagttgacct gtcagatata aaaataatac
    18061 attattaatt tgcatatata gtgcttttgt aaaggaacag gtttactatg agcaatgtaa
    18121 aaaaagcata tctaagcatt cttatttata ctatcattag tattattctt tcttctatag
    18181 tatatttatc aggtgggatc ccatatggca agggatttgc aattggatgc cttttgagtt
    18241 taatcttaac actactatgg attgctggtg caataaaagg catgaagtct aatactctgg
    18301 ttttgctgtc aataaccgca ggcggctttt tgctacggct tactctttta gcggtttttg
    18361 ctgcaggcgg tgtttacatc ttcatgatgg atttgccagc ctttgctata gcatttctta
    18421 ttggcactat ttacagttta gttctggaag tatggttttt taccacgctg agcggtccgc
    18481 aaaatcctaa gttcaggtaa aaaaagtgtt atggatacaa aaacattaca gcaactttca
    18541 caggatgaat taatcaaatt aattcagcag cttcaattaa aaattgatct tttgcaaaag
    18601 ggcgtagaag aagaacccgt tgactataaa aagctcattg aagctgcgga tgacatcatc
    18661 tttgtgctgg acagcgaagg caatgtagtc tatcgcaaca gagccatgga agaccttttc
    18721 cctgttaaca gtaaagacgc cgtcggcatg catttttcat tatacctgcc tgccattgaa
    18781 ttagacagag ccaccatggt gtttaatcag atactctatg agggtaaatc catccataat
    18841 gaaaagatga agacgtttga taaggacgga aatcctgttt acttaatggc aaaccttgct
    18901 cctatcaaag atgatgacgg aaaaattacc ggattatttg gcatactgcg taatatcacc
    18961 gaaatgcacc tgatggaaaa aaaactgaaa gaaagctccc gaaggctgga agaaaagatt
    19021 aaagagcaac tacgacaggc agaagaatta aaacgtctat caactttaaa cgatgagatc
    19081 attaataatt caccaatagg tatatttacc atagacccaa cagggataat attgagtgaa
    19141 aatcccgcgc tgagacgcat aatagggtat gggcaggatg aaacccgtgt tggtatgaac
    19201 ctaaccgaat tgccaggatt tagtgttgac ttaaaaaagt atttagaaga aattttatta
    19261 caaaaaaaac ctgtttttat taaaaacctt acctatcgcc cggtagatca ggataaagag
    19321 cttacattaa atgtcagagc aaatcccata ctggattttg ataaaaaggt aaaaagcatc
    19381 ttaataatga tcgaagatgc caccgaacag gctcaaatcc aaaaacgtat gcaacgtgct
    19441 gaaaaactat cagccatggg actgttagct gctggcgttg cctatgaact gaaagttccc
    19501 attaatctat taactatcga ccttaacttt attgaaaata atatccctga aaccagccca
    19561 atccatgatt atgtcaaatc catgaaggac gaactcagca gaatcaatca gattactgaa
    19621 caactgctga atctgggcaa accgggagat gatgatgtag aagtttttga aattcacaag
    19681 cttataacca gtcatcccat acagattaca ctcaatcgtt tacagaaaag cggctttatc
    19741 atcaacaccg cttatccaaa agaaagtcct aaaattaaag gcagaaaaaa tcaacttata
    19801 caggtattgt tacacattat cagtaatgca gaagaagcaa tgcctgataa aggcgaaata
    19861 agaatcaatg ttggatcggt gattaaaaat ggggaaaaat ttgcaaccat taccatagag
    19921 gatacgggaa ttggaatccc atctgaaaac cttgcaaaga tatttcagcc atttttcaca
    19981 accaaaggac ccaaatcaac tggattggga ctgatggtta cctataccat tataaacaac
    20041 catggcggag caataggggt aaaaagcacc cctggtgaag gcacatcatt taaaatttta
    20101 cttccagctg ttgactaata gggggaccac atgaaatcac aaaacccttc tcatcgaaat
    20161 tatctcagcg atgcagaatc actccttgat caggagggat atatcatcat cgcgcctgac
    20221 gaagataaac aggaattgca gtccgaggat aaacccacaa ctaaaaccga gcatgtcgta
    20281 acccaacagg ttgaaaaact gcccggtaaa aaacctgaaa ttgtaacgaa aacaattgaa
    20341 gcagaagcta ctgatattcc cagcaccatt cagtacctga aatggcatat ggagcgcatt
    20401 gctaaaaatg taatcaacag agaagaaata ataaaacagg ctatgtacgc tatcttaaca
    20461 cgcgagcata tgcttttatt atcacgcacc ggtatggcaa aatcatatct tgcaaattat
    20521 atcttcaaca cgtttgataa tgtcagagtt tttgcctcac aagcaagtaa agaacaaaca
    20581 cctgataact attttggccc ttacaatatt gaagaattta aaaaagggcg tatacggcat
    20641 aatattcacg gatcaattat tgaagccaac cttgtttttc tggatgaatt ttttgatgca
    20701 agtgatgttg tattacgctc tcttttatca gttctgaatg agcgtaaatt cataaatggc
    20761 cctgaaataa ttgatgcagc catccacacc gctattgcaa cagcaaacta catgcgcatg
    20821 aatgaagtaa cggaagctat tttagaccgt tttacctata aatccataat tcccgaagat
    20881 gacaatgtat atcaacaact tcttattgac cacacctatg cgtttagtcg cggtaaagcg
    20941 atagaacctg ataaaaaaat tccatttaat cagattattt atctttcaga tattgttcgc
    21001 aataaaagta aagaagtaaa aattgaaatc cctgattttg tatattttat gaaaaatgtt
    21061 attaccaata aatttgtaag cgatatgcga aaatcagatt ataagttttt tgtaagcccc
    21121 cgaaaacagg caaagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaag
    21241 tgatattatg gtcattgacc gttaccgaac catttacgat ttaaaaaaag cgctccccag
    21301 agaattagca tgggaagata caatttttga tatcaagctt tttaaacacg aattacttgt
    21361 acaaaaattt tacgaagaga aagctgatac ctttaaacca atatccacgc tacaggatga
    21421 aagtgggaga caggcaaata ggtttgatca aaaattttat ttgttacttg accgttcacg
    21481 atccatggaa atcaggatgc gatcgttttt ttcaaaatgt cttgtggcag aatttttacg
    21541 acgcaagctt aacagcaatg caaaaatatt ttaccgtcct tttgattcaa aaccaggtga
    21601 cctttttaaa atagaaaaaa aagaagactt ccctttttta attgaaaggg ttttacttac
    21661 aacaaccggt gggacaagca ccaacttgca gcaagctgtg tttcaagcca tcgatgacat
    21721 taattttgat aaagatatgc tgaattcaga aatactggtt gttaccgatg gtatcagcaa
    21781 aattgacaaa tatttattaa aggagaagct gggcgatatt aaacttaata tcttgaaggt
    21841 tggccgtgat ctggctgagc ctgattattt tgaaatgaaa aaaacatttg atgctaacaa
    21901 aatgaagttt gatcctactg ctcttaatat taaagagata caacagaaga tggaaaaatc
    21961 tcaggatggt gaagaagcat tatccccatt gcaaaagcgt gcttaccgat atatccttga
    22021 atattctgaa accatgttta aagatttaaa agagatatca catcgttttg ttgaaattcc
    22081 cgatttagat ccttcatcat tatttgagct tactgatgaa aagttagatg ccattgaaac
    22141 agcagtagat gattttaccc gaatagatta caacactaaa acattggaag aacgcaaaaa
    22201 actttttaag caagcttatt tcttatctca gtacgtgaca ttgttgcttg aaaataaaaa
    22261 aaattccagt aatcctatcc ttaaaaactg tgcctataag ctcaggaaga tcactcaaac
    22321 tatattagca gatccatcgt tgttgcaaat ggtaatggaa accggacgct ttactgataa
    22381 agatacatta aaactttcaa tgaaacagtt aaaagaaaaa ttaaaagata tgaagatgtc
    22441 acaaacacca ctttcagccg aagaaataaa aaaagcacag atgttactaa catccgattt
    22501 gggcgagggc aatataggac agtttttaag agttcttttt attaaattat tggagttcat
    22561 taaaaaacta atgcaaaaaa taaaaaacgc tatcaaaaag aataattaga gtttttcttt
    22621 cctataccaa ttcaaagttt caacccagat ttcaatatta tcttttttag gaatccattt
    22681 aagttcttct tttattttat tacaatccaa tacaaaattt gacaggatga actgtacatg
    22741 atcttttcgc aaataatgaa tatgcagagg ttttaataaa attgataata tccttgtaaa
    22801 taaaggtgta atatgcttaa tctgacaatc taattgagct atctctttta ccttgacaac
    22861 ctgttccaat tgagttggga cattatccga cccaatattg tacactttgc ctatggaaat
    22921 aggattacgg acaattgata gcaaagcttc aaccacatca tcaggatgta caagttgata
    22981 tcgtgaatca ccgtcaccgg caatatataa tctgtttgaa tcacccattg ccattgccat
    23041 atataaaata ataaggatca ttgcatcgtc tacaccggg
//
LOCUS       DAOOJH010000030        22681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2315_length_22681_cov_9.908557, whole genome shotgun sequence.
ACCESSION   DAOOJH010000030 DAOOJH010000000
VERSION     DAOOJH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22681
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_22681_cov_9.908557"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PLJ75_05815"
     CDS             <1..777
                     /locus_tag="PLJ75_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04768.1"
                     /translation="GESKEMIRQRQENELREKQRQEALQKRRAEIDAVINDLKTQYQD
                     PFDLSDEKNCELFARAYNNPEENPKIRNSTDTSENEFQYQKDKIQYSQLRNKKYAIMS
                     FNIFGNELQKYDFQKKSFSLQFKSYTIMAPEGNVIYTKKRPPVVKLLVKGVPDIDIKI
                     EPDLAENFKSGITNTATGFGAMYGLESQYKDRAILYVIFKINGKILDDDIQHISVDIA
                     DYVCFWYIDAGSSISQGGRYGNSQCSDIIECSSDIKIFRK"
     gene            797..1084
                     /locus_tag="PLJ75_05820"
     CDS             797..1084
                     /locus_tag="PLJ75_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04769.1"
                     /translation="MTKIEKLLKIGPYQLEVTGIDDGRSYIVNVFYKKYYIPPTYSYN
                     HTDFNNIVNSQESGLFYGKLDDVMSDFIRIIETDTMIENKIKQIDKYIESK"
     gene            1819..3525
                     /locus_tag="PLJ75_05825"
     CDS             1819..3525
                     /locus_tag="PLJ75_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPD04770.1"
                     /translation="MKDPKKDQACIERYKELLPNLADYVAHYAKKKPQELAIIEHNTG
                     AKVTWKQFNTSVSAFAAKLLSIGLNKGDIIATSLPLLKEHVYLMYACYRSGIIIAPLD
                     LRLKTKEIQYCLDRMKPKAYFFLGKTPVADFRPIIAEVMKTSPYIKHWVQFQTEPEFI
                     MEGAIGITDFVKDIKTVFIKSLFTGSVRRARKKVQKRDASLIIFTTGSTGSPKPALLC
                     HEGILTQNIGLAIGFELRESDRFLVNLPPSHVGCTTEQLATTIYGGGTSVLLHIFDPK
                     LSLEAIQTHKITCIGQIPALYNMEWLLPNYKDYDLSSLRFAIYGGQAVSREFLVKMKS
                     MSPQIGTGLGLTETSGFCTYTDVNADVDEIVKSIGYDMPLCPLSIREPMNSDGSAGKE
                     KSKGEIGEICFSGPQIFLGYLGDPENTRKTISTDGICYTGDLGYYDEEGLHFSGRSKL
                     VIKPKGYQVYPDDVEHHISEKLKGKVNMVAIVGAEHNVYSEAIIAFVEPLPGVEVTPE
                     DVMKACEDISAYSRPSHVEIIKPGQFPLNRVAKTDYVVLKDEAKKIVEKLRSEGKWDK
                     AK"
     gene            3715..4590
                     /locus_tag="PLJ75_05830"
     CDS             3715..4590
                     /locus_tag="PLJ75_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04771.1"
                     /translation="MERTDIWYCTDNDKGKEIYETLKLLGLNIVLVEDKNFSSCNVLP
                     SQINIFIIDLVTVEFSEILSMLRKDERLHKFIKYLILFKKQIRKASQLSIDVMHLEIL
                     SRPIKKREFSLLLEKSIIVERYKEMLNLYSLEATERIEAYTNLLDINRKESFFDEKEK
                     NIFSKIINFEKHILKEQKELNVAIANYTRLRQKELFDLKKRIDAEEMLSELRRKELMD
                     AKSVIEAQSAVIEYSSKELQSAEEIIHATEAVQELSRLEAIKLHEELEKEKKKNEVLQ
                     KKIDMLEKKINNSKS"
     gene            4686..5141
                     /locus_tag="PLJ75_05835"
     CDS             4686..5141
                     /locus_tag="PLJ75_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04772.1"
                     /translation="MITMQNLFSKRDLITLYNEYVHPDIKSDVLTLIDAMKNEKTDDR
                     KIALRLGFANLNQYYSASHQDIINHFFLMMVSSRYTKPSDIKNSAYLRLAISLSLLFA
                     FLDNGMALVEKDIGFRCAKLTYNGNSFTMKIYFYYSNKKWLLTDGRHCL"
     gene            5190..5456
                     /locus_tag="PLJ75_05840"
     CDS             5190..5456
                     /locus_tag="PLJ75_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04773.1"
                     /translation="MEWQKILVDAVQDGKIKELHLKKIPVLKTCDNWREVEPIGWIDH
                     QMKLTHYKGALVRLRGNIYFVTEKTINALSEYINWKISQRIDVI"
     gene            complement(5460..7262)
                     /locus_tag="PLJ75_05845"
     CDS             complement(5460..7262)
                     /locus_tag="PLJ75_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04774.1"
                     /translation="MRTLLQHINSLNSKERTIIASNLGFHNPMKASVKDIYSRMASYM
                     GIELIFSQCSAEEIAILKLLSVSDLHYRDIEHTLHIPIQQIEKIAQSLTKKSITYILK
                     NRQLLSNKLDRICLFNEIRQHINLLDINNFQNYCKQIIKQLVINKKNDETLLNISESA
                     KRFFTLCIHRGGIVSAAYAIQTLGINKVFGILDELTKNNVISIGHIVTGRFYSIIMAN
                     PDYMSYKIMEKSNFINNHFMALIAMLDSYDCIISKGLFLTQEGNLRKSDFDKIMKSIQ
                     PISIHTTKSRLTNQQTAQLSLYLLYILKCLTISQTNVNAILTPIKKMLLNPHQIITQI
                     LNSLSAPEHQLFVNPFNIKSDNLHEILHIIKKYSNSTIQLCTYIYFITSLPELLFHSQ
                     LNIDDYYDTIEILTNNTIDFLTALGIVEIKNNKVALNDIGRSILLHEKKSDITTRSIY
                     INPDFSLVIPYHEVPSEVSYILLSFTEIISTDVTVYSKFSKTAMLHAIKRGMNPDIFI
                     NTLEQYTRNQFPQNIKFQLNEWIDHTPTITIQRGIILTSTTKGFIDELSHSQIKNVIV
                     ERLGENHVMIHESQLDAVIKYAQKKEAVINIYLQ"
     gene            7395..8465
                     /gene="lepB"
                     /locus_tag="PLJ75_05850"
     CDS             7395..8465
                     /gene="lepB"
                     /locus_tag="PLJ75_05850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD04775.1"
                     /translation="MLQYLALNKQNTDTIQINLPDNIFIIRLMLKRIGYLILLIYSTI
                     FLAVAIPFEATYAIGGFLCCIFLLRIINDFIKWKKFGNVSISISPSGITVTTKNETYH
                     FNAKDITYLEVNPFSNFVIRERHRSLSLPLMLLHKEDREKLLNYFLDMSPKRTVMFKK
                     IWEMFDAILVAFILAMHIRQFIIQAYYIPTGSMEDTLLVGDHLMAEKITYGPTIPRMI
                     GMEAPVHLSFLSIRGVQRGDIIIFRPPNEEDKDFIKRCIAVEGDDVHIEDGAVWINGV
                     KLDEPYVKGTTSYRGFSEKRIEGIVPKGMIVAMGDNRENSYDSRGFGYVSLDRIKGKA
                     FILYWNTDNIKRLDFSRFGLIK"
     gene            8481..10304
                     /locus_tag="PLJ75_05855"
     CDS             8481..10304
                     /locus_tag="PLJ75_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD04776.1"
                     /translation="MENISHKRRILLTLVFVSFIAIVLIFNVTRLHFSTKVIVQNKND
                     TIKRGDIVDVNGYPLALSIYKYSIYADPRKVDNPEEVAQKLSSVVALSYNELKDLLTR
                     DKHFVWIKRKIDDAVFQQVQQLSLHGIYYKKEYARVYPEGSMAAQVLGFVNIDNIGLE
                     GIEYKYNDILLSGDNSQNEENKGLTVQLTLDREIQYIAQKTLNAYSEQVMPQRAIALV
                     QEIKTGKILGLATYPQFNPETFYNYDRSILTCFSVVDSFEPGSTMKLFAAVTLLEHLP
                     DALKKTFICKGSIDVFDATIKCTKEHGRVNLNDIITYSCNAGIIQAMQYVKKDDFYQT
                     LVRFGFGEKTGIQLSGESSGILRPPKTWSGLSKFSMAIGQELSVTSVQLTSAIAAIAN
                     KGIYMYPQIVNQIENTNGNVVSAFFPRSKGKILNEHIAQSILLMMRNVVIEGTGKKAS
                     VAGYETGGKTGTAQKSEHQGGYIPNVYVVSFIGIAPLENPDICVLILFDEPHRGGSGG
                     ELAAPVFSDIVKRILPLRGFGRGTVKQQSPSPVSYTKKKIMHVPNFIGYKDAEALREL
                     INIQQNYPIQYIMKGSGTVVSQDPQPGLPLTKVTTISLTME"
     gene            10307..12157
                     /locus_tag="PLJ75_05860"
     CDS             10307..12157
                     /locus_tag="PLJ75_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPD04777.1"
                     /translation="MENYFQKNISAIVTINPLVANTINKARDNLSIEVVQSKTGKPIP
                     IIKKEHTITVHSRFDPEKEADRLIEEFDCVHYDCIIVSGFGFGYHIEKILQKLKPDAT
                     LLILERFAWMVKTAMCNRDLQSIFHDKRLILLINPSDDDIAVTMKGRSSYKTLFITHR
                     GSFQIDPEGYTNLQRIAKAYLATKEVNIATLAKFEKLWTSNIAKNIKTIIAHPPASAF
                     FNKFTTIPAIIVNAGPTLTESIPFIKENCNNALIIAVDTTYKVLEYYNIQPHFCITVD
                     PQLVNARYFEGAHENKTILIADPMVHPSTFLFYKGRKAITGVAFEMMQWIETIIGDYG
                     KLRYGGSVSTNAYDFAKRLGANPIVMVGQDLAFTRGLAHVKGAYLDEHVYLRTNRFYT
                     VEMFNRFQLTALPKIFVKGIKSKQVHTNQKMMIFISWFSRLHDSALINATYDGAYIDT
                     IVHKNSADISLSCNNVNIRDIIDTVYTETMPSSEIISHRSLKMIEQLNQMLSQTTVMN
                     TMLQQALRYSDDLINNIKMKNSKAITSLINKLNDIDRSISNLTITKNIVGLTIQHVIH
                     TVMEGYEIDETIKDEQEATALRSKYLYTGLRDGLQFSKRIIENMIMLLSQ"
     gene            complement(12158..12913)
                     /locus_tag="PLJ75_05865"
     CDS             complement(12158..12913)
                     /locus_tag="PLJ75_05865"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPD04778.1"
                     /translation="MYPILFQYKFITIGGYGVMLAIGIYLAYLLLERELILRNINPDI
                     AYKILLVAIPAGIIGSKVFHILDHMDEFLADPTGTVFSGAGLSIYGGLLFALFFAYFI
                     IKRSNQPVLKILDIASPCIAIGYGFGRIGCHIAGDGCYGIETSSILGVAYPDGIVPIS
                     YTVYPTPLFESFFSFIVAIVLLQLRKKELPQGMLFFIYLIFNGVSRFCVEFIRRNPVV
                     FAGLTQAQIIAFCFVIAGIAGIVFINKQKLKKA"
     gene            complement(13113..14672)
                     /locus_tag="PLJ75_05870"
     CDS             complement(13113..14672)
                     /locus_tag="PLJ75_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HPD04779.1"
                     /translation="MANVFCRDTIGDIVRRSSLRFGEKIAISFRNTNLSFKQLEEECC
                     KFAHLMQQYGIEKGDRVAMISYNSHYYPISFVGLSKIGAIQVPINYMLNADEMAYIIN
                     HSRSRLVIIEDNLYNVIASLVDKCPAVKNWSYIPLEGGDIPEGFDNLITMLNDMSSAD
                     IYTDVYAEDIAQIPYTSGTESKPKGAMLSHRALMSQYFSCIIEGEYKPDDISLHALPL
                     FHCAQLHCFLMPFLYIGATNVILHKADAVGMMKLIEQYHINHMFSPPTVWIGIINHPE
                     FKNYNLNSLKKAAYGASIMPVEVLKRLTEVFSGLRLWNYYGQTEMGPVATILKPDDQL
                     KKPGSAGKPVLNVETALLDDDGHYVSTGEIGEIVHRSGHVMTGYLDDSEKTSEAFAFN
                     WFHSGDLGKFDEDGFLYVVDRKKDMIKTGGENVASREVEEVLYQHPAIAEVAVFGLPD
                     PKWIELVCAAVILKKDMQATNEEIIEFAKQRLAGFKTPKKIFIMDALPKNPSGKILKR
                     ELKHTLSVNVN"
     gene            15089..18607
                     /gene="nifJ"
                     /locus_tag="PLJ75_05875"
     CDS             15089..18607
                     /gene="nifJ"
                     /locus_tag="PLJ75_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPD04780.1"
                     /translation="MAKRIMKSMDGNEAAAYISYAFTEVAAIYPITPSSPMAEFIDEW
                     AAHNKKNIFGQVVRVVEMQSEAGAAAAVHGSLQAGTLTTTYTASQGLLLMIPNMYKIA
                     AELLPAVFHVSARSISAQALSIFGDHQDINAARQTGFAMLASGGVQEVMDLAGVAHLS
                     AIKSRIPFLHFFDGFRTSHEIQKIEILSYEDLARLIDYNKLNEFKNNALNPEHPVTRG
                     TAQNPDIYFQTREVSNIFYQTVPDIVEEYMQEISKLTGREYHPFIYYGAHDAEHIIVA
                     MGSITETIRETIDYLLQQGEKVGLIICHLYRPFSTQYFFKVLPESVKRIAVLDRTKEP
                     GSIGEPLYLDVRAAFYGMDNCPTIVGGRYGLSSKDTTPAHIIAVYNNLKLNDPKNNFT
                     VGIVDDVTFTSLPVPEDTSVVPEGTFEGKFFGLGSDGTVGANKNSIKIIGDNTELYAQ
                     AYFAYDSKKSGGVTVSHLRFGKKPIRSTYLVNNPDFVACHVPAYLEKYDILKGLKKGG
                     AFLLNSLWDEEETKKQLPDHIKRFLAENNIKLYIINATQISENIGLPGRTNTIMQAAF
                     FKVSNVIPFEIAEKEMKKAIEKSYGRKGADVVSKNMAAVDEGAKQVKEVAVPDAWKTI
                     AVPGKPVFEELPPFIQEIVIPMNSLEGDSLPVSAFLPRPDGTFPPGTTQYEKRGIAIN
                     VPEWQPDNCIQCNQCAYVCPHAVIRPFLLNDDEMRKAPEGMLTLDAKGKGLEGLRFRI
                     QISPLDCTGCGNCAEECPAKEKALIMKPLQREIEQQKYWDFIVKNVTYKDNLINKFTV
                     KGSQFCQPLFEFSGACAGCGETPYIKLITQLFGDRMMIANATGCSSIYGGSAPATPYC
                     INAEGRGPAWASSLFEDASEYGYGMYLGVTQQQNTIAQLLEQVAKTTSNTQLKAASLE
                     WLAGRNNAKASREATQKLLPLLKGDSSLEASEVLKLAQYLEKKSIWIFGGDGFAYDIG
                     FGGLDHVIASGEDVNILVLDTEVYSNTGGQSSKATPIAAQAKFATSGKRVRKKDLGMM
                     AITYGYVYVAQVAMGASQMQYMRAITEAENYPGPSVIIAYAPCINHGLREGMGKSQLE
                     EKKAVECGYWSMWRYNPLLEKEGKNPFILDSKEPNWDKFKDFLMGEVRYSALKKEFPE
                     VAEDLFNAALDSAKWRYNNYKRLASN"
     gene            complement(18695..18862)
                     /locus_tag="PLJ75_05880"
     CDS             complement(18695..18862)
                     /locus_tag="PLJ75_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04781.1"
                     /translation="MINTITNETNINQTSALQKSKDIGKSIVEESRNRLGEVSKTLEE
                     ENKGKNINTQA"
     gene            19393..20751
                     /locus_tag="PLJ75_05885"
     CDS             19393..20751
                     /locus_tag="PLJ75_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPD04782.1"
                     /translation="MINVDSVVLRRKPSYPLSQWHNDYNNKKVTAEEAAKIIQSGDRL
                     FMGGGTGIPASFATALGKRAGTVNNVTIYQGYAMGLYDYMKPENKESFHIETMFVGPM
                     ERVCMEWKMAHYKPMHLSDIPEAGLDARCNKVAFTATPPDEDGFMNLSCFGAFLPKKE
                     VLCKADTIIAEINTNTPWLNSVDFLVHVSEVDYIIEHDTQLFELPEIPITDVEKKMAE
                     MIADLIPDGATIQLGLGGLANGIGNLLYTKKDLGMHAEVITPSVMRLIEAGVLNGINK
                     TLYPEKVVGAFAVGTKEFYQYLDHNNNFLFCDIGWVNTPDVIALNDNFVSINNALILD
                     LTGQVAAESIGTKQYSGTGGQVDFIRGAKKSKGGKSIIALSSTYTDKQGKIHSNIVPL
                     LPLGTVVTTSRTDVEYVVTEYGVANLRMRSIYDRVHALISIAHPDFRDELKYEAAKIG
                     WI"
     gene            20944..22056
                     /locus_tag="PLJ75_05890"
     CDS             20944..22056
                     /locus_tag="PLJ75_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04783.1"
                     /translation="MEYTIVQYNKHHQIIINEYNRKDFIAVCSELLYRPKKLFIGLGD
                     FLEIGDYNQLLDSIFYDEESAIIENFLDAINRKLSEEGQIPFSFLKLAVYYPDYLLKD
                     VPTQCAITVGSKTRLFPGAVHFINFINNYNPMILTAIPYEIANEIVKRVGSDDNHLVS
                     TEYKKIISQKNITLFAGDIEKFISGERKIIEIEKYMALSNTKDEDVLYVGRGESSVIT
                     FSKINSIAFNPSDSIIPHSHITVYGSSLESLLVLFNDSPEINKFLLSEIGEHILPSLI
                     VLSDVKEKPADIIELEKQHRRLQANIIGQRIEHDIESYTTLKRDIELSLSAYPFNFQA
                     VKEMVKERLEKFKINPKEFIDEICSIAQKRYQNWGM"
     gene            22086..22580
                     /locus_tag="PLJ75_05895"
     CDS             22086..22580
                     /locus_tag="PLJ75_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04784.1"
                     /translation="MDNDKAAGILICTIIVLFGIHSVTFALNIGYNQLMLGKQYNDVK
                     DILHYEEYEYNEFVIGSIRVIQYHNHEIDGLLIFDLFNRLYTIIVTLPVDEKQYSQLH
                     EHLTKKYGTFTLDNAMYCCTIGIYRIGLYYNKATNMVTIEYNHTLPLFTPAEKQVYNP
                     FSKF"
BASE COUNT         7771 a         3465 c         4204 g         7241 t
ORIGIN      
        1 ggtgaaagca aagaaatgat ccgtcaaagg caagaaaatg aattgagaga aaaacagcga
       61 caagaagctc tacagaaaag aagagcagaa attgatgcag taataaatga tttaaaaaca
      121 caatatcaag atccatttga cctttcagat gaaaaaaatt gtgaattatt tgctagagcc
      181 tataacaatc cagaagaaaa tcccaagatt agaaattcga ctgatacttc ggaaaatgaa
      241 tttcagtatc agaaagataa aattcaatat agtcaattac gaaataaaaa atatgctata
      301 atgagtttta atatttttgg taatgaatta caaaaatatg actttcaaaa aaagtctttt
      361 tcattacaat ttaaatcata tacgattatg gctcctgaag gtaatgtgat ttataccaaa
      421 aaaagaccac ctgttgttaa attacttgtc aaaggtgttc ctgatattga tattaaaatt
      481 gagcctgacc tcgctgaaaa ttttaaaagc ggaatcacta atacagcaac tggttttggt
      541 gcaatgtatg gcttagagtc acaatataaa gaccgagcca ttttatatgt tattttcaaa
      601 atcaatggaa aaattttaga tgatgatatt cagcatataa gtgtagatat tgcagattat
      661 gtttgctttt ggtatattga tgcaggttca agtatatcgc aaggcggcag atacggaaat
      721 tcacaatgtt ctgatataat tgaatgttct tcagatatta aaatatttcg taaatgaaat
      781 tatttggagt taaattatga ccaaaattga aaaattattg aaaattggac cttatcaact
      841 tgaggttacg ggaattgatg acggcaggag ctatatagta aatgtttttt acaaaaaata
      901 ttatattccc ccgacatatt cttataacca tactgatttt aataatatcg ttaatagcca
      961 agaatcaggt cttttttatg gtaaattaga tgatgtaatg tctgatttta taagaataat
     1021 tgaaactgac actatgattg aaaataaaat taaacaaatt gataagtata tagaaagtaa
     1081 ataaatatat ctccagcctt cgccatcctt ggctacggct ggaataaact ttttgttgtt
     1141 gttggccgca tcacttaaca aagcttatgc gcttcactcg ctgatggtca ttcggactgc
     1201 gccacaattc gcatggcggc tgaacgttgc atgtgaaaaa caatatgcta tattataaac
     1261 cataccatgc ccaacttaac gggataacta tgatactgga ttagacggct tcgtactatc
     1321 aatgttgtag acgtcatata acacgtagcc attcaatgtg ctcattttgt cagttccgga
     1381 ttctgcaaca tttttagcta cactgtatta cggtgaaaaa tcgaatgtac cctaaaagtt
     1441 atgaaaaact gccggtaaaa ctattagttt gccgtcaatg atgggtaagt gggcaaatga
     1501 atttatacga tcataaagta tcattattag cgagtataaa tagagaacaa gaaatacttg
     1561 ataacaaaat taatttttta aacttaccta tatcaaagat aaaccaaaaa tcataaattg
     1621 tataatatgt atatataatt ttatatataa ttatttactt gcaattattt atagtatatt
     1681 atcatattgt catatcagaa ttattttaat actttagtat tttcacaatg ataacaagat
     1741 ggttttgcat ttatgctttt tattataaat agtatcacaa tgtattcata actatatttt
     1801 atatgcagga ggtactatat gaaagatcct aaaaaggacc aggcgtgtat cgaaaggtat
     1861 aaggagttgc ttcctaacct tgctgattat gtagcgcatt atgcaaagaa aaagccacaa
     1921 gagctggcaa taattgaaca caatactgga gcaaaggtaa cctggaaaca atttaataca
     1981 tcagtttcag cgtttgcagc aaagcttttg tccataggat tgaacaaagg agatattatt
     2041 gcaacaagtt tgccattgct caaggagcat gtttatttga tgtatgcctg ttatcgttct
     2101 ggcattatta ttgcaccact ggatttgcgc ttaaagacaa aagaaattca gtattgcctt
     2161 gatagaatga aacctaaagc gtattttttc ctgggcaaaa ctcctgtagc tgatttccgt
     2221 ccaatcattg cagaggttat gaaaacatct ccctatataa agcactgggt acagttccaa
     2281 actgagccag aattcatcat ggaaggtgca ataggtatta ccgactttgt taaagatatt
     2341 aaaacggtat ttatcaaaag cctttttaca ggcagtgtaa gaagggctcg gaaaaaagtg
     2401 cagaaaaggg atgccagctt gatcatattt actaccggtt ctactggttc acctaaacct
     2461 gcattgttat gtcatgaagg tattttaaca cagaatatag gattggctat tggttttgaa
     2521 ttaagagaaa gcgatagatt tttggtaaac cttcctccat cacatgtggg gtgtacaacc
     2581 gagcagctgg caacaactat ctatggtggc ggcacatcgg ttcttcttca tatatttgat
     2641 ccaaaattaa gtctggaagc aattcaaacg cataagataa cctgtatagg gcagatacct
     2701 gcattgtata atatggagtg gttgcttcca aactataaag attatgatct atcaagtttg
     2761 cgatttgcaa tctatggtgg gcaggcagtt tcacgggagt ttctggttaa gatgaaaagc
     2821 atgtccccgc aaattggaac agggcttgga ttgaccgaaa cttcaggatt ttgcacttat
     2881 actgatgtga atgcagatgt tgatgaaata gtaaagagta ttggatatga tatgccgttg
     2941 tgtccactca gtattcgaga acctatgaat tctgatggct cagcaggtaa agagaaatcc
     3001 aaaggagaga taggcgaaat ttgctttagt ggaccacaga tttttcttgg atatcttggg
     3061 gatcctgaaa atacccgtaa gaccatatct accgatggta tatgttatac aggtgatctg
     3121 ggctattatg atgaagaggg gttacatttt tcaggaaggt caaaactggt aataaaacct
     3181 aaaggatatc aggtgtatcc agatgatgtt gaacatcata taagtgagaa gttgaaaggt
     3241 aaagtaaata tggtagcgat agttggtgct gaacataatg tatattccga agcaatcatt
     3301 gcatttgtag aaccattgcc gggagttgag gtaacaccag aagatgttat gaaagcatgc
     3361 gaagatatat cagcgtattc acgaccttct catgttgaga ttattaagcc agggcagttc
     3421 ccattaaata gggtagcaaa aacagattat gttgtgttaa aagatgaagc aaaaaaaatt
     3481 gttgaaaaac tacgaagcga aggcaaatgg gataaagcaa aataataaaa ttattattta
     3541 gattagatgt tatcaaagag gttatctggc aaaattcatt attactatta aatgaattgt
     3601 aacgatagcc tctttttttt attttaatac tacaataaaa aacagttgca aaaattaatt
     3661 attcttctaa tatattggtt gagatataaa atcatactat aaatggtgtt atgtatggaa
     3721 cggactgata tatggtattg tacagataat gataaaggaa aagaaatata tgaaacatta
     3781 aaattgcttg ggttgaatat tgtattggtt gaagataaaa atttttcttc atgcaatgta
     3841 ctcccttcac agataaatat atttattatt gatcttgtta ctgtagaatt ttcagaaata
     3901 ctgtctatgt taagaaaaga tgaacggtta cataaattta ttaaatatct tattttattt
     3961 aaaaaacaaa taagaaaagc atcccaactt tcaattgatg taatgcatct tgaaatatta
     4021 tcgcgcccca taaaaaaacg tgaattttca ttattattag aaaagtctat aatcgttgaa
     4081 cgttataaag aaatgctcaa tctatattcg cttgaagcaa cggaacgcat agaagcatat
     4141 accaacctcc ttgatattaa cagaaaagaa tctttttttg atgaaaagga aaaaaatatt
     4201 ttttcaaaaa taataaattt tgaaaagcat atccttaaag aacagaaaga gcttaatgta
     4261 gcaattgcca attataccag gctacgacaa aaggagctat ttgatttaaa gaaaagaatt
     4321 gatgcggaag aaatgctttc agagctaagg cgtaaggaac tgatggatgc taaaagtgtt
     4381 attgaagctc aaagtgcagt tatagaatat tcaagtaaag aattacagag tgctgaagaa
     4441 ataattcatg ctaccgaagc agtgcaagaa ctaagcagac ttgaagccat aaaacttcat
     4501 gaagagttag aaaaagaaaa aaaaaagaat gaggtactgc aaaaaaaaat tgatatgctt
     4561 gagaaaaaaa taaataattc caaatcctaa taatgaactg tcaattaata ctaaaaatta
     4621 taattattat tgtcttcgtt ccatcatgtt cattcgcatg gtttaaccgg ggacttgatg
     4681 ctactttgat tacaatgcaa aatcttttta gtaaaagaga tttaataacg ctatacaatg
     4741 aatatgtcca tccggatatc aagagtgatg tattaacatt aattgatgca atgaaaaatg
     4801 aaaaaactga tgatagaaag attgctttac gtcttggctt tgctaatctt aaccaatatt
     4861 atagcgcctc gcatcaggat attattaatc actttttttt aatgatggtt agttcacgat
     4921 atactaaacc ttcggatatc aaaaatagtg cttatctcag gcttgcaatt tctttatcgt
     4981 tgctatttgc ttttcttgat aatggtatgg cactcgttga aaaagatatt ggcttcagat
     5041 gtgcaaaact aacttataat ggtaatagct tcaccatgaa gatatatttt tattatagta
     5101 ataaaaaatg gttgttgaca gatggaaggc attgtttata agaattgtta tagcaataaa
     5161 ataaaataat gattgattag ataataagta tggaatggca aaaaatatta gttgatgcag
     5221 tacaggacgg aaaaataaaa gaattgcatc ttaaaaaaat tcctgtttta aagacctgtg
     5281 ataattggcg tgaggtagaa cccatcgggt ggattgatca tcaaatgaag cttactcatt
     5341 ataaaggagc acttgtgagg ttaaggggta atatttattt tgtgacagaa aaaaccatca
     5401 atgctttgag cgaatatata aattggaaaa tatctcagcg tattgatgta atataattat
     5461 tattgtaaat atatatttat aacagcttcc ttcttttgag catatttaat aactgcatca
     5521 agttgcgatt catggatcat gacgtggttt tcacctaacc gttcaacaat tacatttttg
     5581 atctggctat gggataattc atctataaat ccctttgtag tggaagtaag tataattcct
     5641 ctctggattg taatggttgg agtatggtct atccattcat taagctgaaa ttttatattt
     5701 tgagggaatt gatttctggt gtattgttct aatgtattaa taaaaatatc agggttcata
     5761 cctcttttga ttgcatgtaa cattgctgtt ttggaaaatt ttgaataaac tgttacatct
     5821 gttgagatga tttcagtaaa agatagcagt atataggaaa cctcagaagg gacttcatga
     5881 tatggtataa ccagtgaaaa gtcgggatta atatatattg aacgagttgt tatatcagat
     5941 tttttttcat gaagcaatat tgatctacca atatcattta atgcaacctt attattttta
     6001 atttctacta taccaagggc ggtaaggaaa tctattgtat tatttgtcaa tatttcaatt
     6061 gtgtcataat aatcatcaat attaagctgt gaatgaaaca aaagttctgg caatgatgta
     6121 ataaagtaaa tataagtgca aagctgaatg gtactgttag aatacttttt tatgatatgc
     6181 agaatttcgt gtaaattatc gcttttaatg ttgaaaggat ttacaaaaag ttgatgttca
     6241 ggagcgctta acgaattgag aatttgagta atgatctgat gcggattaag cagcattttt
     6301 tttataggag taaggatagc atttacattg gtttgcgaga tagtcaaaca tttaagtata
     6361 tagagtagat ataatgataa ttgcgcagtt tgctggttgg taagtcggct tttagttgta
     6421 tggatagata taggctgaat ggatttcatt attttatcaa aatctgattt tcgcaggttc
     6481 ccttcctgag ttaagaataa tcctttagat attatacaat catagctatc aagcattgca
     6541 ataagcgcca tgaaatggtt gtttataaaa tttgattttt ccataatttt gtatgacata
     6601 taatctggat ttgccattat aatgctataa aatctgcctg ttactatatg accaatgctg
     6661 atgacattat tttttgttag ttcatctaat atgccaaata ctttgttaat gccaagcgtt
     6721 tgtatagcat aagcagcgga tactatgcca ccacgatgaa tacataatgt aaaaaaacgc
     6781 tttgcacttt cgcttatgtt aagaagagtt tcgtcatttt ttttattgat aaccagttgt
     6841 ttaattatct gcttgcaata gttctggaaa ttattgatat caagaaggtt gatatgctgg
     6901 cggatttcat tgaataaaca aattctatct aatttgtttg atagtaactg tctatttttc
     6961 aatatgtagg taatggattt ttttgttaat gattgcgcaa ttttttctat ctgctgtata
     7021 ggaatatgaa gtgtatgttc aatgtcgcgg tagtgaagat ctgaaacgga tagtaatttt
     7081 aaaattgcta tctcttcagc agaacattga gaaaaaataa gttctatccc catatagctt
     7141 gccatgcggg aatagatatc tttaactgaa gccttcatgg gattatgaaa gccaaggttg
     7201 gaagcaatta tggtgcgctc tttgctattt aatgaattaa tatgttgtaa tagtgttcgc
     7261 attatttaaa aagtagtaga cacattgatt gcaataatat acatgatatc atcttggctt
     7321 tacaataaaa aatgtaactt ttatcataac tatgcatata tatactatat actataaaaa
     7381 tttgaggtac atagatgtta cagtatttag cactcaacaa acaaaatacc gatactatcc
     7441 agatcaatct gcctgataat atatttatta tcagacttat gttgaaacgg ataggatatc
     7501 ttatattatt aatttattca actatttttt tagctgtagc aatacctttt gaagctacat
     7561 atgctatcgg tggatttcta tgttgtatat ttttgctaag aataatcaat gattttatta
     7621 aatggaaaaa atttggaaac gtttctatta gcatttcgcc atcagggata actgttacta
     7681 caaaaaatga aacgtatcat tttaatgcaa aggatataac gtatttagaa gttaatccat
     7741 ttagtaattt tgtaataaga gagcgtcaca gaagtttaag tttaccctta atgctgttac
     7801 ataaagaaga tcgagaaaag cttttaaact attttctgga tatgtcacct aaaagaactg
     7861 ttatgtttaa aaaaatatgg gagatgtttg atgcaatcct tgtggcattt attcttgcaa
     7921 tgcatatccg acaatttatt atacaggcgt attatattcc aactggatct atggaagata
     7981 ccctgcttgt tggggatcac ctcatggcag aaaagattac ttatggccca actatcccca
     8041 gaatgattgg aatggaagcg ccagtacatc tttcattttt gagtatacgg ggagtgcaaa
     8101 gaggtgatat aataatcttc agaccaccca atgaagaaga taaagacttc attaaacgat
     8161 gtattgctgt ggaaggtgat gatgtacata ttgaagatgg tgcggtgtgg attaatggtg
     8221 ttaaattgga tgaaccgtat gtaaaaggaa ctacatcata tagaggattt agtgaaaaac
     8281 gcatagaggg aatagttcct aaaggaatga tagtagcaat gggtgacaac agggaaaatt
     8341 catatgatag ccgtggcttt ggatatgttt cccttgacag gatcaaaggt aaagcattta
     8401 ttttatactg gaatacagac aacataaaac gtcttgattt ttcaagattc ggactaataa
     8461 aatagagctt ttaagatgat atggagaaca tatcgcataa acgaagaatc ctgcttacat
     8521 tagtctttgt aagttttatt gctatagttt taattttcaa tgttacacgg ttgcatttca
     8581 gtacaaaagt aattgtacaa aataaaaatg atactataaa gcgaggggat atagttgatg
     8641 taaatggcta cccccttgca ctatcaatct ataaatattc aatttacgcc gatcctcgca
     8701 aagtagataa tcctgaagaa gttgcacaaa aactttcatc ggttgttgct ctatcctata
     8761 acgaattaaa agatttatta acgcgtgata agcattttgt atggattaaa cgtaagatag
     8821 atgatgcagt ttttcaacag gttcaacaat tatcgttgca tggcatatac tataagaagg
     8881 aatatgcacg agtctatcca gaaggttcaa tggctgcgca ggtacttgga tttgttaata
     8941 ttgataacat agggcttgag ggtattgaat acaaatataa tgatatacta ttatcgggcg
     9001 ataactcaca aaatgaagaa aacaaagggt taacagttca actgacatta gatagagaga
     9061 tacagtatat agcgcaaaaa actcttaatg cttattcaga gcaggtgatg ccccaaagag
     9121 ctattgctct ggtacaggag attaaaacag gtaagatact gggattagcc acctacccgc
     9181 aatttaatcc ggaaacattt tataattatg atagatcaat tttaacatgc ttttcagtag
     9241 tggattcatt tgaaccaggt tcaacaatga agctatttgc tgctgtaaca ttacttgaac
     9301 atcttcctga tgccttgaaa aaaacattta tttgtaaagg gagcattgat gtgtttgatg
     9361 caaccattaa atgcactaaa gaacatggca gggttaattt aaatgatatt attacctact
     9421 catgtaatgc aggtatcatt caggcgatgc agtatgttaa aaaggatgat ttctatcaaa
     9481 cacttgttcg ctttggattt ggagaaaaaa ccggaattca actttcaggc gaatcgtcag
     9541 gaatactcag gcctcctaaa acatggtcag gtttgtcaaa attttcaatg gcaataggac
     9601 aggaactgtc ggtaacttct gtacagctta catctgctat agcagctatc gccaataaag
     9661 gaatatatat gtatcctcag atagtaaatc aaattgaaaa tacaaatggt aatgttgtat
     9721 cagcgttttt ccccaggagc aaaggaaaaa tattgaatga acatattgcg cagtctattt
     9781 tgcttatgat gcgtaatgtt gttattgaag gtaccgggaa aaaagcatct gtagctggct
     9841 atgaaacagg tggtaaaacg ggtacagcgc aaaagtcgga acaccaggga ggctatatac
     9901 ccaatgttta tgttgtatca tttataggta ttgctccgct tgaaaatccc gatatatgtg
     9961 tactgatatt atttgatgaa ccacaccgtg gaggatcagg cggggaatta gcggctcctg
    10021 ttttcagcga tattgtaaag cgaatattgc cattgcgagg ttttggcagg ggaaccgtta
    10081 agcaacaatc gccatcaccg gtgtcatata caaagaaaaa aattatgcat gttcccaatt
    10141 ttattggcta taaagatgct gaagcactgc gcgagcttat aaatattcaa caaaattatc
    10201 cgatacaata tataatgaaa ggttcaggta ctgtagtttc tcaggacccg caaccgggat
    10261 taccattaac taaagttacc accatatcgt taaccatgga gtagatgtgg aaaattattt
    10321 ccaaaaaaat atttcggcga tagttactat taatccactg gttgctaata caataaacaa
    10381 agcccgtgac aatctttcta tagaagtggt gcagtctaaa acaggaaaac caatccctat
    10441 aataaaaaaa gaacacacca taacagtcca cagcaggttt gaccctgaaa aagaagctga
    10501 ccgcttaata gaagaatttg attgtgttca ctatgattgt attattgttt cagggtttgg
    10561 atttggatac cacattgaaa aaattttaca aaagttaaaa cctgatgcaa cgctgctgat
    10621 attggaacgt tttgcatgga tggttaaaac agcgatgtgc aatcgtgatt tgcagagtat
    10681 attccatgat aaaagactta tactattgat taatccatct gacgatgata ttgcagtaac
    10741 tatgaaaggc agatcatcct ataaaacctt atttattaca catcgcggtt cgttccagat
    10801 tgatcctgaa ggctatacca atttacaacg aattgcaaaa gcttatctgg caaccaaaga
    10861 agtaaatatt gccacattag caaaatttga aaaactgtgg acctcaaata ttgctaaaaa
    10921 tattaagacc ataattgcgc atccgccggc atcagcattt tttaataaat ttactaccat
    10981 ccctgcaatt attgtgaatg ctggtccaac tttgactgaa agcataccgt ttataaaaga
    11041 aaattgtaat aatgcgttga tcatagcagt tgatacaacg tataaggttc tagagtacta
    11101 taatatccag ccacattttt gcatcaccgt tgatcctcag cttgtcaatg cgcgatactt
    11161 tgaaggagcg catgaaaaca aaaccatact cattgcagac ccaatggtac atccttcaac
    11221 ctttttattt tacaagggaa ggaaagcaat taccggtgtt gcatttgaga tgatgcaatg
    11281 gattgaaacc atcattggag attatggcaa gcttagatat ggcggatcgg tttcaaccaa
    11341 tgcctatgac tttgcaaaaa ggttaggagc taatccaatt gtgatggttg gacaggattt
    11401 agcgtttacc agagggcttg cccatgttaa gggagcatat ttagatgagc atgtatattt
    11461 acgaacaaat cgtttttata ctgtcgagat gtttaaccga tttcaactaa ccgcattacc
    11521 aaagatcttt gttaaaggga ttaaaagcaa acaggtgcat accaatcaaa agatgatgat
    11581 ttttatatca tggttcagca ggttacatga ttcagcatta atcaatgcta cgtatgacgg
    11641 agcttatatt gatactattg ttcataaaaa ttctgctgat ataagtttgt cttgtaataa
    11701 tgtcaatatt cgggatatca ttgacactgt ttatactgaa acgatgccat catcagagat
    11761 tatatcccat cgttcattaa aaatgattga acaattaaat cagatgcttt cacaaacaac
    11821 ggtgatgaat acaatgttgc aacaggcatt acggtattct gatgacttaa taaacaatat
    11881 taaaatgaaa aattcaaagg ctatcacatc acttataaac aaattaaatg acattgatag
    11941 atcaatttca aacctaacta tcaccaaaaa tattgttgga cttactattc aacatgttat
    12001 tcatacagtt atggaaggtt atgagattga tgaaacaata aaggatgagc aggaagcaac
    12061 agctcttcgc tcaaagtatt tgtatactgg cttgcgtgat ggattacaat ttagcaaacg
    12121 tataattgaa aatatgatta tgctattatc acaataacta agctttcttt aatttttgtt
    12181 tattaatgaa aactatacca gcaataccag caatcacaaa acaaaatgca attatttgag
    12241 cctgggttaa accagcaaaa acaaccggat tgcggcggat gaactcaaca caaaagcgtg
    12301 acacgccatt gaaaattaaa tagataaaaa aaagcattcc ctgagggagc tcttttttcc
    12361 ttagttgaag caacactatt gcgacaatga atgaaaaaaa ggattcaaat aatggagtag
    12421 ggtacacggt gtatgatatt ggtacaattc catcagggta tgctactcct aaaatacttg
    12481 aagtttctat tccataacaa ccgtcgccag caatatgaca tcctattctg ccaaaaccat
    12541 agccgattgc tatgcagggc gaagcaatat caagtatttt taatacaggt tgattactgc
    12601 gtttaatgat aaaataagca aaaaataggg caaatagtaa tccaccatat attgaaagtc
    12661 cggcacctga gaaaacagta ccggttgggt cagccaaaaa ttcatccatg tggtcaagaa
    12721 tatgaaatac ctttgaacct atgatacctg cagggatagc tacaagaaga atcttgtatg
    12781 ctatgtctgg atttatattt cgtagtataa gctctcgttc taataaaaga taggctaaat
    12841 aaataccgat tgcaagcata acaccatatc caccaattgt tatgaatttg tattggaata
    12901 gtattggata catcgtatta ccccttattt ttcagttttg taataatatc tgatcgctat
    12961 ctctaaagtc tatatagttc tttgaaaata tatgagtaat tcagtttaat gataatgaga
    13021 agtttttata ggtgacacta aatagataat aaaaaaaaat tgcaacagaa ttattgtacc
    13081 tgctgcaatt tttaagaata tgaagtgtaa tattaattta cattaacaga taatgtatgt
    13141 ttcaattcac gttttaatat ttttcctgaa ggatttttgg gtaatgcatc cataataaag
    13201 attttctttg gggttttgaa tccagctaaa cgttgttttg caaattcaat tatttcttca
    13261 ttagttgcct gcatatcttt tttcaatatc acggcggcac agactaattc aatccattta
    13321 ggatcaggaa gtccaaatac agctacctca gcaattgcag gatgctgata taaaacttct
    13381 tcaacttcac gagatgcaac attttcgcca cctgttttta tcatatcttt ctttctatct
    13441 acaacataaa ggaatccatc ttcatcaaat ttacctaaat ctccactatg aaaccaatta
    13501 aatgcaaatg cctctgaagt tttttcagag tcatctaaat aaccagtcat aacatgtcct
    13561 gaacggtgaa ctatctctcc aatttcacca gtagaaacat agtgaccatc atcatccagt
    13621 aaggcagttt caacgttcaa cacaggttta cctgctgaac caggtttttt taattggtca
    13681 tccggtttta agattgttgc taccggtccc atctcggttt gtccataata gttccacaac
    13741 ctaagaccag aaaaaacttc tgttaaacgt tttaatactt caacaggcat aatactggca
    13801 ccatatgcag cttttttaag actgtttaag ttataatttt taaattcagg atggttaata
    13861 ataccaatcc ataccgttgg tggagaaaac atatgattaa tatggtactg ttcaattaat
    13921 ttcatcatac caactgcatc agctttgtgc aatatcacgt tagtagcgcc aatatataaa
    13981 aacggcataa ggaaacaatg aagttgagcg caatggaaaa gtggtaatgc atggagactt
    14041 atatcatcgg gtttgtattc tccttctata atacagctaa agtattgtga cattaatgct
    14101 cggtgtgata gcatcgctcc ttttggtttt gattctgtac cgctggtata aggaatctga
    14161 gcgatgtctt ctgcataaac gtcagtataa atatctgctg atgacatatc attcagcatt
    14221 gtaataagat tgtcaaatcc ttcgggaata tcaccacctt ccaatggaat ataactccaa
    14281 tttttcacag ctggacattt atcgactaat gaagcaataa cattgtataa attatcttct
    14341 atgataacaa gcctggatct ggaatgatta ataatatatg ccatttcatc tgcattgagc
    14401 atgtaattaa ttggtacctg aatagctcca atttttgaaa gtcctacaaa acttattgga
    14461 taataatgtg aattgtaaga aatcatagct accctgtcac ctttttcaat accatactgt
    14521 tgcataagat gagcaaattt acaacattcc tcttcgagct gcttaaatga aagattggta
    14581 ttccgaaatg aaattgctat tttttcccca aaacgtaaag aagagcgtcg tacaatatca
    14641 ccaattgtat ctctgcaaaa aacattagcc ataaagatcc tccgtttata ttatgatatg
    14701 atatataaca aatatgaaat aatacctttt gcattcaagc aatatttgta aataaaatat
    14761 aaaatagtca aagattggat agaatggact attgcatatg agaattaaat ggaattgtta
    14821 tatattattg ctatttttta ttaataatag gatataatat aaacattggt agaattataa
    14881 ttagattttt actatatttt tgatctttat taattgtttt ttttattgac ttgtttcata
    14941 atgatttatg aaggtaataa tcacgccctg atctttttct aatcatacat agtagcaatt
    15001 gtttatattt aataggtgtt aatgcaggct gaattgattt tttatgttta taattattat
    15061 ataataaaac agaaaggaga aagctttcat ggcaaaacga attatgaaat caatggatgg
    15121 taatgaggct gccgcgtata tttcatatgc tttcaccgag gtggctgcca tctatccaat
    15181 tacaccatct tcacctatgg ctgaatttat agacgaatgg gctgctcata acaaaaagaa
    15241 catatttgga caggtagttc gtgttgttga gatgcaatca gaagctggtg ctgcggccgc
    15301 agttcatggt tcattgcagg caggtacatt aactacaacc tatactgctt cacagggttt
    15361 acttttaatg atacctaata tgtataaaat tgccgctgag ttattaccag cagtttttca
    15421 tgtaagtgcg cgttcaattt ctgctcaggc actatcaata tttggtgatc atcaggatat
    15481 taatgctgca cgtcaaactg gttttgctat gcttgcaagt ggcggagtgc aggaggttat
    15541 ggatttagcc ggtgttgctc atctaagtgc tatcaaatca cgcataccgt ttttacattt
    15601 ttttgatgga tttagaacat ctcatgaaat acaaaaaatt gaaatacttt cttatgaaga
    15661 tttagccagg ctgatagatt ataataaatt aaatgaattt aagaataatg cattgaatcc
    15721 agagcatccg gtaacacgtg gcacagctca aaaccctgac atctattttc aaactcgtga
    15781 ggtaagcaat atattctatc agactgtacc tgatattgta gaagaatata tgcaggagat
    15841 aagcaaatta actggtagag agtatcatcc atttatttat tatggtgctc atgatgctga
    15901 acatattata gtggctatgg gatcaattac tgaaaccatc agagaaacaa ttgactattt
    15961 gttacagcaa ggagaaaagg ttgggcttat aatatgtcac ctgtaccgcc cattttcaac
    16021 acaatatttc tttaaagttt tacctgaaag cgtaaaacga atagctgtcc ttgatagaac
    16081 caaggagcca ggttcaattg gtgagccgtt atatttagat gtcagagctg ctttttatgg
    16141 tatggataat tgcccaacta ttgttggcgg acgatatgga ttaagctcaa aggatacaac
    16201 gccagctcat attatagcag tatacaataa tttaaagctc aatgatccca aaaataactt
    16261 tacagtaggt attgtagatg atgtaacatt tacttcatta ccagttccag aggataccag
    16321 tgttgttccc gaaggtacgt ttgagggtaa gttttttggc ttgggttctg atggaacggt
    16381 tggtgccaac aaaaattcta taaagataat aggtgacaat acagaactgt atgctcaggc
    16441 ttattttgcg tatgattcaa aaaaatcggg tggtgtcaca gtatctcatc ttcgatttgg
    16501 taaaaagcct atccgttcta cctatctggt aaataatcct gattttgtag cctgtcatgt
    16561 accagcatat cttgaaaaat atgatatcct caaagggttg aaaaaaggcg gggcattcct
    16621 gcttaattca ttatgggatg aagaagaaac taaaaagcaa ttacctgatc atataaaacg
    16681 atttctggct gagaataata ttaaattgta catcatcaat gcaacacaga tttcagaaaa
    16741 tattgggctc cccggcagaa ccaatactat tatgcaagca gcatttttta aggttagcaa
    16801 tgttataccg tttgagattg ctgaaaaaga gatgaaaaaa gctattgaaa aaagttatgg
    16861 caggaaaggt gcagatgttg ttagcaaaaa catggctgcc gttgatgaag gtgcaaaaca
    16921 ggttaaggaa gtagcggtgc ctgatgcatg gaaaactatt gcagtgccag gaaaaccggt
    16981 atttgaggag ttgccaccat ttatacaaga aatagttata cccatgaata gtcttgaagg
    17041 tgacagcctc cctgtaagtg catttttgcc aaggcctgat ggcacatttc ctccggggac
    17101 aacgcagtat gaaaaacgcg gaatagcaat caatgttcca gaatggcagc ctgataattg
    17161 tatacaatgt aatcagtgtg cgtatgtttg ccctcatgct gttatacgtc catttcttct
    17221 caatgatgat gaaatgcgta aagctcctga agggatgcta acactggatg ctaaaggtaa
    17281 aggacttgaa ggactacgat ttagaattca aatcagtcca ctggattgta ccggatgtgg
    17341 caattgtgct gaagaatgcc ctgcaaagga aaaagctctt atcatgaagc ctcttcaaag
    17401 ggaaattgag cagcagaaat actgggattt tattgtcaaa aatgtaacct ataaagataa
    17461 tcttatcaat aaatttactg tcaaaggtag tcagttctgt cagcctttat ttgagttttc
    17521 tggtgcatgt gccggttgcg gcgaaacccc ttatattaaa ctcataacac aattatttgg
    17581 tgaccggatg atgatagcta atgctaccgg ttgttcatca atctatggag gctctgctcc
    17641 tgcaactccc tattgcatca atgcagaagg acgtggtcct gcatgggcaa gttcattatt
    17701 tgaagatgca agtgaatatg ggtatggcat gtaccttggt gtaacgcagc agcaaaatac
    17761 catagctcag ttgctggaac aggtagcaaa aactacaagt aatacacagc ttaaagcagc
    17821 atcgttagaa tggcttgcag gcaggaataa tgcaaaggct tcacgtgaag caacgcaaaa
    17881 gcttttaccc ttattaaagg gcgatagttc cctggaagca agcgaggtgt taaaattagc
    17941 tcaatacctt gagaaaaaat caatctggat atttggtggc gatggatttg catatgacat
    18001 tgggtttggt ggattagatc atgtcattgc atctggcgag gatgttaata ttctggtatt
    18061 ggatactgag gtatattcca acacgggagg acagtcatcc aaagctacac ccattgcagc
    18121 gcaggcaaag tttgcaacaa gcggtaagcg tgtacgaaag aaagatcttg gtatgatggc
    18181 tattacctat ggttacgtgt atgtggctca ggtagcaatg ggtgccagcc agatgcagta
    18241 tatgcgagct ataaccgaag cggaaaatta tccaggacct tccgtaatca ttgcatacgc
    18301 accatgtatc aatcatggat tacgcgaagg aatgggtaaa tcacagcttg aagagaaaaa
    18361 agctgttgaa tgtggctact ggtcaatgtg gcgttacaat ccattgctgg aaaaagaagg
    18421 gaaaaatcca tttatactgg actccaaaga acctaattgg gataaattta aagatttttt
    18481 gatgggtgaa gtgcgctatt ctgctttgaa gaaggaattc cctgaagttg ccgaagacct
    18541 ttttaatgca gcactggata gcgccaaatg gcgttacaat aattataaac gattagcgtc
    18601 aaattaagaa taataaaggc tccctgtaat aacctataat aatagggagc ctttttaata
    18661 ctttatcctt tggatatcac ctcgtaaaat atttctatgc ctgagtattt atattctttc
    18721 ctttattttc ttcttccaga gttttagaaa cctcgcccaa tctgtttcgg gactcttcaa
    18781 ctatgctttt ccctatatcc tttgattttt gtaacgcact tgtttgattg atgtttgttt
    18841 cattagtgat tgtattaatc ataaaccctc ccttgttttt tgctgatttg ttgaaatggc
    18901 ataattgaaa tctaacattt attaatatat tactttaaaa aactatcgcc acttattatt
    18961 ttactttaat atatataaga taggtaataa agtcaaataa attatgtata aatgtaatag
    19021 attgctttta ttatttaaaa accattatat gactatttgc aacgcaacca atcaaaatca
    19081 tcaatgatta tggatgtcaa ttattgagta tatggcaaaa gtgaattaca ttaaatgtaa
    19141 ataaatagaa aatggaagaa atttcatacc aaataaaatt ttattaccag taactatcgc
    19201 cataaaaata aatggaaaaa atatacaaat atatataata tataatgtat atgcatataa
    19261 attaatattg ataatattaa attttctatt gataaatata ataatatcta tatgtattgt
    19321 taagttgata aatgagcagt acagtaacta caatggatag tagttaaaat ttttaaatta
    19381 ttgcggtata gtatgataaa cgttgattca gttgttttgc gtcgcaagcc atcgtatcca
    19441 ctgtcacaat ggcataatga ttataataac aaaaaggtta cagcagaaga agctgcaaag
    19501 attattcaat ctggtgacag gctgttcatg ggtggtggta ctggtatccc tgcttcgttt
    19561 gctaccgctc ttggtaaaag ggcagggacg gttaacaatg taaccatcta tcagggatat
    19621 gcaatgggtt tgtatgatta catgaaaccc gaaaataaag aaagttttca tattgaaaca
    19681 atgtttgttg gtcctatgga acgtgtttgc atggaatgga agatggcaca ttacaaaccc
    19741 atgcatttaa gcgatatacc cgaggcagga ttggatgcaa ggtgtaacaa agtagcgttt
    19801 acggcaacac cacctgatga agatggattt atgaatttat catgctttgg agctttttta
    19861 ccaaaaaaag aggtgctatg caaagctgat actattatag ctgaaataaa tacaaatact
    19921 ccatggctca atagcgttga ttttcttgta cacgtttctg aggtggacta tatcattgaa
    19981 catgatactc aattatttga actgcccgaa attcccataa ctgatgttga aaagaagatg
    20041 gctgaaatga ttgcggattt gatccctgat ggagcaacca tacagcttgg tttgggtggg
    20101 cttgcaaatg gaattggcaa tttactgtat acaaaaaaag atttagggat gcatgctgag
    20161 gttattaccc catcagttat gcgacttatt gaggcaggtg ttcttaatgg tattaacaaa
    20221 acactttatc ccgaaaaggt tgttggcgca tttgcagtgg gcacaaaaga attttatcaa
    20281 tatcttgatc ataacaacaa ttttttgttt tgtgatattg gatgggttaa tacaccggat
    20341 gttatagctt taaatgataa ttttgtttcg atcaataatg cgctcatact tgatttaacc
    20401 gggcaggttg ctgctgaatc cattggaaca aaacagtata gcggaactgg tgggcaggta
    20461 gattttatac gtggtgctaa gaagtcaaag ggtggtaaaa gtattatagc gcttagttca
    20521 acatataccg ataaacaagg aaaaatacat agcaatattg ttcccctttt gccattgggc
    20581 actgtggtaa caacatcacg aactgatgta gaatatgtag tcacagaata tggtgttgcc
    20641 aatcttcgta tgcgaagtat ctatgacaga gtacatgcat tgatatcaat agcgcatcct
    20701 gattttagag atgagctgaa atatgaagct gccaaaatag gttggatata atatatcata
    20761 aagtaattga attaatttct tgaaaaattc ttaatgacct gtaattctat tttatagaat
    20821 attacaatat cctatttatt ctattataat gatattgtaa tgtttattga tgtttaaagt
    20881 attactattt atttatttag ttagttagtt agttagttag ggaagatcat taagagttta
    20941 tatatggaat atacaatagt tcaatataac aaacaccatc aaataattat caacgagtat
    21001 aatcggaaag attttattgc tgtttgctct gagttgctat accgacctaa aaagctgttt
    21061 ataggacttg gtgattttct tgaaattggt gattataacc agttgctgga ttcaattttt
    21121 tatgatgagg aaagtgcaat cattgaaaat tttttagatg caataaacag gaagctctca
    21181 gaagaagggc aaattccatt ttcttttttg aaattagcag tttattatcc tgattattta
    21241 ttaaaagatg ttcccacgca atgtgctatt actgtcggtt ccaaaacacg tctttttcct
    21301 ggtgcagtgc attttataaa ttttatcaat aattataacc caatgattct aacggcaatt
    21361 ccttatgaga tagctaatga aattgtaaaa cgtgttggtt ctgatgataa ccatttagtg
    21421 tctactgaat ataaaaaaat tataagccaa aagaatatta cattatttgc tggtgatatt
    21481 gaaaaattta tttctggtga acgtaagata attgaaattg aaaaatatat ggcactatcc
    21541 aatacaaaag atgaggatgt gctgtatgtt ggaaggggag aatcaagtgt tattacattt
    21601 tcaaaaatta attctattgc ttttaatcct tcagattcta taattcctca ttcacatata
    21661 acggtatatg gttcttctct ggaatcgctg ctggtattgt ttaacgattc accggaaatt
    21721 aataaatttt tgttatcgga aattggtgaa catatattac catcacttat tgtattatca
    21781 gacgtcaaag aaaaacctgc cgatattatc gagttagaga agcaacatag aagattgcag
    21841 gcaaatatta ttggtcaaag aattgagcat gacatagaaa gttatacaac attgaaacgt
    21901 gatattgaat tgtcattgag tgcatatccc tttaactttc aggctgtgaa agagatggtt
    21961 aaggaacgtc tggaaaagtt taagataaat cccaaagaat ttatagacga aatctgttct
    22021 attgcacaaa aaaggtatca aaactggggc atgtaatatt tttcttaagg gagggattta
    22081 atggtatgga taatgataaa gcagcaggaa ttttaatatg taccattata gtattgtttg
    22141 gtattcattc tgtaacattt gctcttaata ttggctataa tcaactaatg ttaggtaagc
    22201 aatacaatga tgttaaggat atcttacact acgaagagta tgaatataat gagtttgtaa
    22261 taggttcaat cagagttatt caatatcata atcatgagat agatggatta ttaatctttg
    22321 atctttttaa taggttatat actatcatcg taacactacc tgttgatgag aagcaatatt
    22381 cccagttgca tgaacacctt acaaaaaaat atggcacgtt tactcttgat aatgcaatgt
    22441 actgctgtac tataggtata tacaggattg gattatacta caataaagca actaacatgg
    22501 ttactatcga atataaccat accctgccat tatttactcc agcagaaaag caggtataca
    22561 atccattctc aaagttttga taaagacggt attcagtagt gctcaacaaa agaatgtata
    22621 gtttttccct tcttctcctt ctctatcttt cacagggtat cccttttggt tttcaggtta
    22681 c
//
LOCUS       DAOOJH010000031        22274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2369_length_22274_cov_10.052388, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000031 DAOOJH010000000
VERSION     DAOOJH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 22274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22274
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_22274_cov_10.052388"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..2464)
                     /gene="cas3"
                     /locus_tag="PLJ75_05900"
     CDS             complement(122..2464)
                     /gene="cas3"
                     /locus_tag="PLJ75_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HPD04785.1"
                     /translation="MFRWEIFSVMDSLNFETLKKQIFKKFQLNQYLAKSDKNYKTNTS
                     IEDHVKDLLYELDNFLSKYSDVIKTTDDMLLLRLAILFHDIGKINILFQKKLTENKKD
                     DVIPHNCLSPLFLKLVNKLEEDKLSLLTYIIFNHHKRGANYFSNDSYGINLLIKQIQE
                     NQKYFFNNFEFSEETLENYKVWLLNSLRNRKSKKYIILTGLLIRLDHAASGELEVEEE
                     PIKDDRKDILFSYFKRKNKTTKLRPFQERYGINEKKDYQCIVADTGLGKTGLSVLWSH
                     KKIFYILPNRASVNAMFNTLKEIYGEYNVGLLHSTALFNLIQDNEDDIAVLKEYDQTR
                     VLSKPVTVCTADQLFTAAFNMPGYEKIYATLSYSDVIIDEIQGFQPQQIIPMLKQIKE
                     TKELGANYLIITATLPSIVTNRLMEYGFDVIENDDCTTDPIKRHKIKIEKGKTIEDLS
                     EGIVDTAVSGKKVLVIVNTVKKAQEMYNKINNTTKEHRANFIDVNILHSRYIWKDRQV
                     KENAIIDECEQDNNFNYKKQNYSIWITTQLVEASLDIDFDYLFSEASTADSVIQRMGR
                     VWRHKNVDYNGDENIIIATAVENTVYEKILIEKTVEMIENSIKNNDNILISSEKRRIV
                     NELYSEKNLKQWRSKYIEEWNKFEEQINSGWNFILEENSQRAFRDVMSIDLIPAIYKE
                     IVESSYKKLLDLRNEKSLDGKVKRLQRLQILKEIQEYKVPVPLYLLNPNVSRKLINTS
                     QPIEWIDKGYGIGILHEYYKYDQILGLTCKGIKGEDEIII"
     gene            complement(2425..3057)
                     /gene="cas5"
                     /locus_tag="PLJ75_05905"
     CDS             complement(2425..3057)
                     /gene="cas5"
                     /locus_tag="PLJ75_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HPD04786.1"
                     /translation="MLRLIIYQETAHFRIATIGNPYLTYLLPPPSTIYGFLRKITNYK
                     SINYQNTKLSIQGIYESISLEKERLTLETKREIKTNIIPIQKLHNSTWIIHIDSVYDR
                     EIKEGINDYVGILRLGRVEDLIIDFSLEEIMISDNDYKNIDVDYFIYKKWEQGKDFKG
                     QLFSMFIDSEVDEKLNIIGYKPVQLIYSSVYSSLDEIKCSDGKYLVSWIH"
     gene            complement(3077..4024)
                     /gene="cas7i"
                     /locus_tag="PLJ75_05910"
     CDS             complement(3077..4024)
                     /gene="cas7i"
                     /locus_tag="PLJ75_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015419280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HPD04787.1"
                     /translation="MKLTGVFLIETGVVNRGDGIGNIATVKKITTPQGIKVYFSPQSY
                     KRALWNSLQNNFGWSLPLVTKEGGVVQRTGTVIDSEEFDFGGTMVAKPMYQRNTTFYL
                     DNGISINNFTGDMEFMTNMGIAKLYGENEANIINRENFYGIYLLPFGIEIDRIGIQEL
                     NLDLTVSFESNDNLEQKIEKIYTALKLKEQIDDYKIKMWVNRIASIEIQNNKIIIILT
                     IDERKRRFQQLMEAMGELGRKIEGSERNLCPHFAFFSLTYATPKYLLFIKDKLIRKEK
                     LTEQDIQHYAEKDGKNIIIATHENYKEKIKEIIEAVFPN"
     gene            complement(4051..5694)
                     /locus_tag="PLJ75_05915"
     CDS             complement(4051..5694)
                     /locus_tag="PLJ75_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04788.1"
                     /translation="MNKEKITFTLTGNFQYDLGILGLKRVLDFFGIKYESDKKFYITL
                     QRDANTLLEDIIAKLIFDNGINYFWKKLLEDLKKKNKNNKDIQKKIEELGKVENISCE
                     CDLKENYKDKGLTLLIENISDVLFNILNDDMKNEIGVNYIKDLLWQKSVNLLNNILLN
                     FQADMNIKGEGTLRKAKNKLNDNIVKDSKCSFCNQKMGKRLTRDVFFFAPSQYNAFWF
                     SEPSLYVCPACLVSNFAITQAFTFLGNKLDAIVFYTPNLEDMEKMNVTLTSHFNDNLN
                     FGFFKIVKSLIEYEKEILKKEASVKELQILSFRLDSKNPLIEMFILSEGTIYNLIKIE
                     NQLNSLFDENNISKLFGYIREKDRYAKIDFSKELMKNLSQNQKIIYLVQKYSRYCIMS
                     ETFKQNKTEKPPVRNFTPWLFLNFLKIHFKLEGYMIDEFNKFQEIGQRMRQRVYMLLT
                     DNKTKSINWNTFDNKIISLSNSFLNTSKGSLQQFQELLSRVIISLGLNIDMSIIKIIN
                     KENFGEITTIIALALLVSQPAENRNREDDQMQDIKKEQI"
     gene            complement(5749..6129)
                     /locus_tag="PLJ75_05920"
     CDS             complement(5749..6129)
                     /locus_tag="PLJ75_05920"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPD04789.1"
                     /translation="MKEITKQWIKKAEEDYIVTSREIELDKPVCSAVCFHSQQAVEKY
                     IKAVLQENEIYFPKTHDLEKLMDLCKEYIPELEQLADGLLWLTSYAVEVRYPGITARK
                     KEAIKSYEIAKKVRDMVRRHLDVD"
     gene            complement(6126..6431)
                     /locus_tag="PLJ75_05925"
     CDS             complement(6126..6431)
                     /locus_tag="PLJ75_05925"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04790.1"
                     /translation="MMIIDELANVIKKEFSVQKIILFGSHAYGQPHKDSDVDICVIME
                     TNLPFPEQAAIIRLKLREKIKEKPPIDLIVRTPDSIKEELKKNNFFIRDIIEKGKQL"
     gene            complement(6428..6817)
                     /locus_tag="PLJ75_05930"
     CDS             complement(6428..6817)
                     /locus_tag="PLJ75_05930"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPD04791.1"
                     /translation="MKEITKEWVNKAEKDFAVVLRLLENSDPVFDAVCFHSQQCVEKY
                     LKAVLQENEIYCEKTHDLWLIAQKCLPFISKLNTMKSDLIRLTAYAVEVRYPGFEADK
                     EAAENSLAICTEVRKLVRQYFGLEVAG"
     gene            complement(6814..7134)
                     /locus_tag="PLJ75_05935"
     CDS             complement(6814..7134)
                     /locus_tag="PLJ75_05935"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04792.1"
                     /translation="MNDELYQSLKEISHFIKEKFLAQKVILFGSYAYGNPTPDSDIDF
                     FVIMDTDKRFPEEAANIRIGIDEEFHIAKPIDILVRTPGYVQKRLGMGDFFMKDIIEK
                     GIEL"
     gene            complement(7131..7895)
                     /gene="cas6"
                     /locus_tag="PLJ75_05940"
     CDS             complement(7131..7895)
                     /gene="cas6"
                     /locus_tag="PLJ75_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HPD04793.1"
                     /translation="MRFVVHMHFDNGNDILLPPWYRIGFASMIKEALRQDATSKMYEL
                     YYGNAKKNRPKPFTFAVKLNVADVEKSNVNYLRLGNPFISFYISSNDYEFIMTVYNGL
                     LKLKQYAIYNNTIIIDRFNLLPEKTFPESKAVFDIFSPIVVRKVDENKKGTGYVNVND
                     STYKQMLFYSIRSQCKFLGDSYLLNDDDVAINTSSAFTVKIPHYNKRNPDKPEVIAAT
                     DGCIAIQAPNEVLQLIYDIGLGARRSQGFGMLEVAG"
     assembly_gap    7920..8019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8259..8627)
                     /locus_tag="PLJ75_05945"
     CDS             complement(8259..8627)
                     /locus_tag="PLJ75_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04794.1"
                     /translation="MLLPLFDKDCEHAGWLDPGKYIYDADMNFIAFIINSNAWSVETG
                     NWLGPVQGLLCYDTNGKLMFWNPKEKITGTVNAPRPSRQILATKPQTPAKPAEPAKPA
                     PPKCNANVWSDLSLWGWLSQ"
     gene            9086..9538
                     /locus_tag="PLJ75_05950"
     CDS             9086..9538
                     /locus_tag="PLJ75_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD04795.1"
                     /translation="MKWGLTKRDNTALNPFEEFERDVKKTFENFFSLEPANLFDSAWI
                     PTVDVKEDEKSIHVRAEIPGIDEKDINVALEDNILTISGEKKEERKEENKRYVLSESR
                     YGSFKRCITLPGEVKADSAKAKFKNGVLTIDFEKKEVSQPKRITINVK"
     gene            9550..9960
                     /locus_tag="PLJ75_05955"
     CDS             9550..9960
                     /locus_tag="PLJ75_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD04796.1"
                     /translation="MFGTYDLFDDVFALRNLVDRFFDEAAFSRQRVDFPYVNIYEKDD
                     EILVRAIAPGVKAEDVDVQLIDNTLTIEMTCKEDYANQPYLRKEREFGTFRKSIQLPY
                     QVDPNKVDASLNNGILTIKLSKHEAVKPRKIMIK"
     assembly_gap    10017..10116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10117..10513
                     /locus_tag="PLJ75_05960"
     CDS             <10117..10513
                     /locus_tag="PLJ75_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD04797.1"
                     /translation="KEVNSMATQLAQKPEQNVKKHDYVITPPVDIYETDNEYVLKADM
                     PGVTKDNVNITFNNNELEITGKVDEQYTSNENMTYREYTLFNYYRKFVVSNAINPEGI
                     SASLENGVLTVTLPKREEAKPKKIEIKAE"
     gene            complement(10872..11318)
                     /locus_tag="PLJ75_05965"
     CDS             complement(10872..11318)
                     /locus_tag="PLJ75_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04798.1"
                     /translation="MKKHTIVTMIALSIVLMLLACVGTSEITEKPAAPAFKYYGLDGL
                     YLTTDEKQMNGVAKDHAKLRVGEFVVVYARGMSTVDTGAQWFKLPSSVAVTWKADSEL
                     QVAPASGHTVTVRVVKPIAVTSYVTAVATAKNGKKIEALFTVEGKK"
     gene            complement(11722..12135)
                     /locus_tag="PLJ75_05970"
     CDS             complement(11722..12135)
                     /locus_tag="PLJ75_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HPD04799.1"
                     /translation="MVVNIKKSGLLYVIVLIMVAGCTKDMKEPCTTINAVHYKGEEIV
                     INNGGCISFELESQLSTGHRWFFKIEQNAVVLVNSDYKVETMQQQVVGGIDKELFTFK
                     AVKPGTATIVFEYIRAFDKNKKPVKSKEYKVTIVE"
     gene            complement(12159..13310)
                     /locus_tag="PLJ75_05975"
     CDS             complement(12159..13310)
                     /locus_tag="PLJ75_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPD04800.1"
                     /translation="MKKHIAASITVCMLIAPVAFSHAQMKSSFKASVEAQKQRDAKSI
                     AEVKQQLAIIKKDIKERNLKFKVDMTEALKRQIDEITGAIPPEDLEKEAEIQTNSGLQ
                     LFLEFLQRLSESKAKKQKEATLPPKKEPVKPEEAKKEEPKAVEPEKPRETLPESFMLA
                     DPSKPALDWFALGMMTPIKDQGLCGSCWSFTAMGVVEGCYKIKAKDEIDLSEQYCIDC
                     ARDKYGRDAGSCNGGWYGNVFDFLMNNYPVEEKVAPYRGYEYGCTPKKQFPIKMVAWG
                     YVRADAGIPTVEQMKKALCTYGPIAATVKVTPAFQAYVGGIFDEFTPVSSDKDVNHGI
                     IIVGWDDSKHAYRIKNSWGQRWGEKGYMWIQYGCNNVGYGAAWVVLDKE"
     gene            13711..14553
                     /locus_tag="PLJ75_05980"
     CDS             13711..14553
                     /locus_tag="PLJ75_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04801.1"
                     /translation="MKSIRFLFTVTITVFVLAAVSGIVIAQQQAAPKEPQKEGIKEEV
                     TGWRIKDFKPYIKAIEELEKLSKEYSDNLLKLAIDEYSTGIDILEDMENEVNKLVETN
                     QKNKNLNEQWYWQEIDRREQEKRQISMMKFEAKSKAITYFTRAINHMDEITFEEVRKD
                     PKFVNFQIGLYKAYVSTQYDLYNLKPCIPILERYIAINDQTKNDVWAYKYLASCYGYM
                     ESVLVKYKQGTEQEQLQYKQKKNIYMLKAAELQYGIESPQYKHLREIVSQDEKKSEKI
                     NEFK"
     gene            complement(14623..15627)
                     /locus_tag="PLJ75_05985"
     CDS             complement(14623..15627)
                     /locus_tag="PLJ75_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04802.1"
                     /translation="MILKTGCSTFNCIFWMLLFIFHFCFCLQGNACEAFALHNGVIYL
                     DSNTSLPVNGTVLDFTAINNRAILYVARSEKEVTAGAYLLDEKKSIVCVVASINESSE
                     VKRIAFDPPVVYISIHDSTNCYLYRADVNIGIVQQKEAITDFVLLDGGLAVLTNDNYL
                     EYNDGKLPLYFLSRPYFKRAIDSRIVLVADTDATEVIDVVAQKPVYRYASNVQYVSSE
                     DHTVEIALYDMPSEEKAGRVFYKIMCDGVDYTRTEPGLSINSTAIDLNLTACQYHEII
                     IERWRLDETENKYIRDNNIRQPKPLTVYVYPGRVIVFTMIFNGTEYQVHSHFKVESQK
                     "
     gene            complement(15596..17305)
                     /locus_tag="PLJ75_05990"
     CDS             complement(15596..17305)
                     /locus_tag="PLJ75_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HPD04803.1"
                     /translation="MRLAEPSIEHVQSKIPTDLEHLRKLFIMPDSPDRFMQFGYELLD
                     LIHSFFKEKGGIHSEISLPQLACLFNAIDIPKEPKLLKDILAEVKQKVIKHSVKVGNP
                     YYIGHMTSAIPYFMILLEMIIAALNQNQVKIETAKASTFVEREFISWIHRLIFCKSAS
                     FYKRNIQNHRIALGNVTLDGTLANLTAMLVARNKAFPADERFPGIRKAGVAETFRYYA
                     IHKAVFIVSQRGHYSIDKVARITGIGDNNVIKIPVDSNNKIDINALKKVLKEISEYNS
                     SHHDTIKVISLIGIAGTTETGNIDDLMALRKIADEYNIFFHVDAAWGGSVLLVNKYRD
                     LFRGIEKADSVTFDAHKLLYSPLSMGLILFKNPYDLLNIMHTSDYIIRPDSVDQGRFT
                     VEGSRPFSCLKPWVTLKIFGTTGFEVLFDHAFMLTSTLKDIINKHENFELLNSPELFI
                     TNYRFVPEGVQSKINRIYEQLSNADQQKATRLQKRLKKLNDGINEMNIELHRAIRQED
                     NSFVSRTMLESTRYRGQKIVVLRAITINPLTTPAILQEIIDEHNKLGLTIYHDFKNRL
                     FHI"
     gene            17834..18565
                     /locus_tag="PLJ75_05995"
     CDS             17834..18565
                     /locus_tag="PLJ75_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD04804.1"
                     /translation="MAILKLENVSLSLNNKPILDSISVDFWEGYIHAIVGPNGAGKST
                     LSYVIMGLNDYRTISGTLYFKNKPINELSVYQRARLGISLAWQEPARFEGLTVYQYLQ
                     ASKKDNGLSIEEAMDVIGLPAQEYLPRACDKTLSGGERKRIELASLLVMQPEVVLLDE
                     PDSGIDVEALERIFDAMAILKQRGSTIITITHSMAVLQKAEHAFLMCHGNIIDKGGID
                     KISGYFENKCIPCDHKNIPISGEAR"
     gene            18565..19509
                     /locus_tag="PLJ75_06000"
     CDS             18565..19509
                     /locus_tag="PLJ75_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005489888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HPD04805.1"
                     /translation="MDDRRAVAQTLYKTMGLTDAALNDPDIAHLVINSNMVVASHLVE
                     GLVVEPAETQNGVRVTMRVLAGHIIQKPVHLCFGMLPEDGLQEIIMDVTIEEHASVSI
                     LAHCTFPNAKNITHKMEAVITIKEGASYSYLERHVHAASGFITVLPHAIVTVEKNATF
                     KTDFELLRGKAGIIDIDYETTCYEHSHLEMNAKVNGIQDDAITIRETGHLNGEGAVGV
                     LTTRIAVRDTAQATVYNTLTAHAAYARGHVDCKEIIQDRATAKAIPVVEVYHPKAHVT
                     HEAAIGSVDDKQLQTLMARGLSEDEAVDLIIQGLLSGS"
     gene            complement(19514..19960)
                     /locus_tag="PLJ75_06005"
     CDS             complement(19514..19960)
                     /locus_tag="PLJ75_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04806.1"
                     /translation="MTFDDTKIYAYFTIAMFIVALLFGLPGLYSTFITEPAIESLIYH
                     DNPSSQQLKQAYIMLRKPHIFAGYNRYDEVGAGIEYILKDFDLRVAEKKDFDVNDTMY
                     LELLLERRKQGSDLSIKTMVFFVLLSLLGSIALVIEKTSKNRMKER"
     gene            complement(19957..20418)
                     /locus_tag="PLJ75_06010"
     CDS             complement(19957..20418)
                     /locus_tag="PLJ75_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04807.1"
                     /translation="MKKILIICVGVVCLSCTRYVELGDVEFTVNVTFEKNVPVFASTY
                     TIYINNKSVDTVITSYSAVVSILDEEQKAVKQCTVTAEQILPKQQKQLFVQQKDSEDT
                     IKVLLDALEISVDELIKQREAALVLPARMVRVDNVTYKTVDIVTYLKEVEK"
     gene            complement(20680..20898)
                     /locus_tag="PLJ75_06015"
     CDS             complement(20680..20898)
                     /locus_tag="PLJ75_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04808.1"
                     /translation="MRDDLASNFKAELIWKSGEIIKKQLNEKTDKDHNNKFTFEIGSI
                     ENSAINKHASDTLEGTLNAFNARRLKYI"
BASE COUNT         6891 a         4149 c         3365 g         7669 t
ORIGIN      
        1 attgataaga taatcaatat caatgttaag tttacgtggc atgcgatagt tcctctacta
       61 tactagttgt aataattggg atcaaaacat taagcatagt tttgctaaaa attgaagata
      121 cctaaataat aatttcatcc tcacccttta tgcctttaca tgttaaacct aaaatttgat
      181 catacttata atactcatgc aaaataccta ttccatatcc tttatcaatc cattcaattg
      241 gctgggaagt attaataagt tttctactaa cattaggatt tagtaaatat agtggaacag
      301 gaaccttata ttcctgtatt tcttttaaaa tttgtaatct ttgcaatctt ttgacttttc
      361 cgtctaaact cttttcattt cttaaatcta aaagcttttt gtaactactt tcaacaattt
      421 ctttataaat tgcaggtatc agatcaatac tcattacatc tctaaatgct ctttgcgaat
      481 tctcttctaa aataaaattc caaccactgt ttatttgttc ttcaaattta ttccactcct
      541 ctatatactt tgagcgccat tgcttaagat ttttttctga atataattcg tttactattc
      601 ttcttttttc tgatgaaatt aaaatattat cattattttt tatgctattt tcaatcattt
      661 caactgtctt ttctattagt attttctcat aaaccgtatt ttctacagca gtagctataa
      721 ttatattttc gtccccatta taatcaacat ttttatgtct ccagactctt cccatccttt
      781 gaataacaga atctgctgtg gatgcttctg aaaataaata atcaaaatca atatccagag
      841 aagcttcaac taattgtgta gttatccata tagaataatt ttgtttttta tagttaaaat
      901 tattatcctg ttcgcattca tcaataattg cattctcttt tacctgtcta tctttccata
      961 tatatctgct atggagaatg ttcacatcaa taaaattggc tctatgttct ttggttgtgt
     1021 tatttatttt gttatacatc tcctgagcct ttttaactgt attgacaatt accaaaactt
     1081 ttttacctga tacagctgta tcaacgatac cttcactcaa gtcttctata gttttacctt
     1141 tttctatttt aatcttgtgc cgttttatag gatcggttgt acaatcatcg ttttctatta
     1201 catcaaatcc atattccatt agtctatttg tcacaataga tggcaaagta gctgtaataa
     1261 taaggtaatt agcaccaagc tctttagttt ccttaatttg ttttaacatt ggaataatct
     1321 gttgtggttg aaaaccttgg atctcatcta ttataacatc agaataagat aaagttgcat
     1381 atatcttttc gtatcctggc atattaaaag cagcagtaaa taattggtct gcagtgcata
     1441 ctgttactgg ttttgatagc acacgagtct gatcatattc ttttaatact gcaatgtcat
     1501 cttcattgtc ttgaattaaa ttaaacaatg ccgtagaatg aagtagtcca acgttgtatt
     1561 caccatatat ttcttttaaa gtattaaaca tagcattaac agatgctcta tttggtaaaa
     1621 tataaaatat ttttttatgg ctccacaata cggaaagccc tgttttccca agccctgtat
     1681 ctgctactat acattgataa tctttcttct catttattcc atatctttct tgaaatggtc
     1741 taagttttgt tgtcttattt tttcttttaa aatacgaaaa aagaatatct ttcctatcat
     1801 ctttgattgg ttcttcttcc acctctaatt caccggaagc agcatggtca aggcgaataa
     1861 gaagaccagt taatattatg tatttttttg actttctatt tcttagggaa tttaaaagcc
     1921 aaactttgta attttctaat gtttcttcac taaattcaaa attattgaaa aagtatttct
     1981 gattttcttg tatttgttta atcaaaagat ttataccata actatcattt gaaaaataat
     2041 ttgcacctct cttgtggtgg ttaaatatta tataggttaa caaagaaagt ttatcctctt
     2101 ctaatttgtt aaccaacttt agaaacaatg gtgataaaca attatggggt attacatcat
     2161 cttttttatt ttcagttaat tttttttgaa acaatatgtt tatttttcca atatcatgaa
     2221 acaaaatggc taaccttaat aacaacatat catcagtagt ttttataaca tctgaatatt
     2281 ttgataaaaa gttatctaac tcataaagca aatctttaac atgatcttct atactagtat
     2341 tagttttata atttttatca gacttagcta aatattgatt tagctgaaat tttttaaata
     2401 tttgcttttt tagagtttca aaatttaatg aatccatgac actaaatatt tcccatctga
     2461 acattttatt tcatccagag aactataaac agaagaatat attaattgaa caggtttata
     2521 tccaattata tttaatttct catctacttc actatctata aacatactaa aaagttgacc
     2581 tttaaaatct ttcccctgct cccatttttt atatataaaa taatctacat caatattttt
     2641 gtaatcgtta tctgaaatca tgatttcttc taaagagaaa tctataatta aatcctcaac
     2701 ccgacctaat cgtaaaatac ctacataatc atttattcct tcttttattt ctctatcata
     2761 tactgaatca atatgaatta tccatgttga attatgtaat ttttgaattg gtataatatt
     2821 tgtttttatt tctcttttag tttcaagagt taatctttcc ttttctaagg aaatactttc
     2881 atatattcct tgaattgata gttttgtgtt ttgataattt atgcttttat agttagttat
     2941 ttttctcaaa aagccataaa tagtagacgg tggtggaagt aaatacgtca aataaggatt
     3001 accaatagta gcgatacgga aatgtgctgt ttcttgataa ataattaatc tcagcaattt
     3061 tgacctcctc tatcaattaa tttggaaaaa ctgcctcaat tatttccttt attttttcct
     3121 tgtaattttc atgagtagca ataataatgt ttttcccatc tttttccgca taatgttgaa
     3181 tatcttgttc tgttaatttt tctttcctta ttaacttatc tttgataaac aacaaatact
     3241 ttggagtggc ataagttaac gaaaaaaacg cgaaatgagg gcataaattt ctttcactcc
     3301 cttctatttt cctaccaagt tcacccatcg cttccattaa ttgttggaac ctcctttttc
     3361 tttcatctat agtaagaata ataattattt tattgttctg aatttcaatt gaagctattc
     3421 tgtttaccca catttttatt ttatagtcat caatttgttc ctttaatttt aaagcagtat
     3481 aaattttttc tattttttgt tccagattgt cgtttgattc aaaactaacc gttaaatcta
     3541 aattaagttc ctgaattcca attctatcta tttctatacc gaatggcaaa aggtaaatac
     3601 cataaaaatt ctcacggttt ataatgttag cttcattttc tccataaagt ttagctatac
     3661 ccatattagt cataaattcc atatcaccag tgaaattatt gattgatatt ccattatcta
     3721 aataaaatgt tgtatttctc tggtacatag gctttgcaac cattgtcccg ccaaaatcga
     3781 attcttcact atcaatcacg gtacctgttc tttgcacaac accaccttct ttagtaacta
     3841 aaggcaaaga ccatccaaaa ttattttgta aactgttcca taaggctctt ttatacgatt
     3901 gtggtgaaaa ataaacttta ataccttgag gtgtagttat tttttttaca gttgcaatat
     3961 ttccaattcc atccccccta tttacaactc ctgtctcaat taaaaaaact cctgttaatt
     4021 tcataataat cctcctcaat attttataat ttatatctgt tcttttttaa tatcttgcat
     4081 ttgatcatct tccctattac ggttttcagc aggttgtgat actagtagtg ctaatgcaat
     4141 tattgttgta atttcaccaa aattttcttt attgattatt tttatgatac tcatatcgat
     4201 atttaatcct aaactaataa ttacccttga tagcaattcc tggaactgtt gcaatgatcc
     4261 tttagaagta tttaaaaaag agtttgagag tgaaataatt ttgttgtcaa aagtattcca
     4321 gttaatagat ttagtcttat tatcagtaag aagcatatat actctctgtc tcatcctttg
     4381 ccctatttct tgaaatttat taaattcatc aatcatataa ccctccaatt taaaatgtat
     4441 tttcaaaaaa ttaagaaata accatggcgt aaaatttctg acaggtggtt tctcagtttt
     4501 attttgttta aaagtctccg acattataca atatcttgaa tatttttgaa ctaaatatat
     4561 aattttttga ttttgtgaaa gattcttcat aagttctttc gaaaaatcaa ttttagcgta
     4621 tctatccttt tctctgatat aaccaaataa tttactaata ttattttcat caaacaatga
     4681 attaagttga ttttctatct ttatcaaatt ataaattgta ccttctgata gtataaacat
     4741 ttctattaat ggatttttag aatctaatcg aaaactcaaa atctgaagct ctttaacaga
     4801 agcttccttc ttcaggatct ctttttcata ttcaatcaaa gatttaacaa ttttaaaaaa
     4861 accaaaatta agattatcat taaaatgtga tgttaaagta acattcattt tttccatatc
     4921 ttctaagttt ggagtataaa aaactatagc atccaattta tttcctaaaa aagtaaaagc
     4981 ttgagttata gcaaaattag aaaccaaaca tgcagggcaa acgtataaac tcggttcact
     5041 aaaccagaac gcgttgtatt gagatggtgc aaagaaaaac acatctctcg ttaatctttt
     5101 tcccattttc tgattacaaa aggaacattt agaatcttta acaatattat catttaattt
     5161 attttttgct tttcttaaag ttccttcccc ttttatattc atatcagctt gaaaatttag
     5221 tagaatatta tttaataaat taacactttt ttgccataat aaatctttga tataatttac
     5281 tcctatttca tttttcatat catcattcaa aatattaaac agtacatcag atatattttc
     5341 tataagcagg gttaaaccct tgtctttata gttttctttt aagtcacatt cacaagatat
     5401 attctctact tttcccaatt cttcaatttt tttttgaata tctttattat tcttattctt
     5461 tttcttcaaa tcttccaata gctttttcca aaaataattt ataccattgt caaatataag
     5521 ctttgcaatt atatcttcaa ggagtgtatt agcatctctt tgtaaagtaa tataaaattt
     5581 tttgtcactt tcatatttta ttccaaaaaa atctaatact ctttttaagc caagtatacc
     5641 caaatcatat tgaaaatttc ctgttagtgt aaaagtaatt ttttctttat tcatcccact
     5701 acccccgcgt tgtatagcca gtttgatgga taaagtataa tttcattatt aatcaacatc
     5761 cagatgcctc cttaccatat ctcgtacctt tttagctatt tcatatgatt taatggcttc
     5821 ttttttcctg gctgttatac caggatacct tacttctact gcatagcttg ttaaccatag
     5881 taatccatct gctaattgtt caagctctgg aatatattct ttgcataaat ccattaactt
     5941 ttctaaatca tgggtttttg gaaaatatat ttcattttcc tgaagaacag ctttaatgta
     6001 tttttctaca gcttgctgag agtgaaaaca tactgcacta cataccggtt tatccagttc
     6061 tatctctctg ctggttacta tataatcttc ttcagccttt tttatccatt gttttgttat
     6121 ttctttcaca attgtttccc tttctcaata atatcccgta taaaaaaatt gtttttcttt
     6181 agttcttctt taatagaatc aggggttctt acaataaggt ctataggggg cttttctttt
     6241 atcttttctc gtaatttcag gcgtataatt gctgcttgct ctggaaatgg taaattggtt
     6301 tccataataa cacatatatc cacatcgcta tccttgtgag gctgcccata ggcatgtgaa
     6361 ccaaaaagaa ttatcttttg tacactaaat tcttttttta ttacatttgc taattcatca
     6421 atgatcatca tcccgctacc tccagcccaa aatattgcct tacaagcttt cgtacttctg
     6481 tacatatagc cagcgagttt tccgccgctt ctttatcggc ttcaaaacct gggtatctaa
     6541 cttctacggc ataggcggta aggcgtatca aatctgattt cattgtattt agtttagaaa
     6601 taaaaggcaa acatttctgt gcaatcagcc ataaatcatg tgtcttttca caatagattt
     6661 cattttcctg taaaactgct ttaaggtatt tttcaacaca ttgttgggag tggaaacata
     6721 cagcatcaaa aactggatcg gaattttcaa gcagtctcaa aacaacagca aaatcttttt
     6781 cggctttatt tacccactct ttcgttattt ctttcataac tctattcctt tttcaataat
     6841 atccttcata aaaaaatcac ccatacccag tcttttttga acataaccag gggttcttac
     6901 caggatatct attggttttg ctatatggaa ttcctcatct attccaattc taatatttgc
     6961 agcttcttca ggaaatcttt tgtcagtatc cattataaca aaaaagtcta tatcactatc
     7021 aggtgttgga ttgccatagg cgtaagaacc aaaaagaatc accttttgtg ctaaaaattt
     7081 ttcttttata aaatgggaaa tttctttcaa actctggtac aattcatcat tcatcccgct
     7141 acctccagca taccaaatcc ctggcttctt cgtgctccca gccctatatc gtatataagc
     7201 tgcaacacct cgtttggtgc ctgtattgca atgcacccat cggttgctgc tataacctcg
     7261 ggtttatcag ggtttctttt gttataatgc ggtatcttta cggtaaatgc actgctggta
     7321 ttgattgcta catcatcatc gtttaacagg taactatcgc ccaaaaattt gcattgactc
     7381 cttatgctgt aaaaaagcat ctgtttatag gtactatcgt taacattgac ataccctgtt
     7441 ccttttttgt tttcatcaac ctttcttacc acaataggcg aaaaaatatc aaacactgct
     7501 tttgactctg gaaatgtttt ttcaggtaaa agattaaacc tatctatgat gatggtattg
     7561 ttatagattg catactgttt aagttttaat aatccattgt agacagtcat tataaattca
     7621 taatcgttgc ttgaaatata aaaactaata aacggattac caagtctaag atagttaaca
     7681 ttgctttttt caacatcagc aacattgagc tttactgcaa aggtaaaagg ctttggtctg
     7741 ttttttttgg cattaccgta atagagctca tacattttac tggttgcatc ctgccgcaat
     7801 gcttctttaa tcattgatgc aaagcctatc ctgtaccatg gcggcaacag gatatcatta
     7861 ccgttatcaa aatgcatatg aacaacaaaa cgcatagaac ctcctcttta ttatatatgn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt atcaaaatgc atatgaacaa
     8041 caaaacgcat agaacctcct ctttattata tatgatgttc atcaggcgtt attgatcgga
     8101 attttttaac tttctcccat attatttata gtatatacta tactcattac gtgggacata
     8161 tagtgtccct gtaataattt ttttcacaat gcagtaaaaa tttttacagg ctatagttcc
     8221 gtcccacaac acaaaataaa caatagatgt tatgcgtgtt actgcgaaag ccagccccaa
     8281 agtgataagt cagaccatac attagcatta cactttggcg gtgccggttt tgcaggttca
     8341 gccggtttgg ctggcgtttg tggtttggtg gcaagaatct gtcggcttgg acggggagcg
     8401 ttaaccgttc cagttatttt ttcttttgga ttccagaaca tgagttttcc gtttgtatcg
     8461 tagcataaaa gcccctgcac cggtcccagc caattgccag tttcaaccga ccatgcattg
     8521 ctattaataa taaatgcaat aaaattcata tctgcatcat aaatatattt accggggtca
     8581 agccatccag catgttcaca atctttgtca aataacggta acagcattgt gcaaacctcc
     8641 ttatactatt ttatatggtg acatgcaggt atttttcata ccaggaaaat cctttataaa
     8701 gacaatacta tatataagaa tttttcaaca aaaaaattag ttaaccatca actatttgta
     8761 gaataaatat tcattagtat agatacatgg tgtaataaca tatgcacccg ttctgctgta
     8821 atagtatctt tttcaatgtg ttagttcaat acctcagtga ttattatact atgtcatcat
     8881 gccacctctg gtatgattct ctgtgtttta acgcaaattg gtgaaaaatg taatagattt
     8941 ctggcaatat tttatcgttt agtgaaaata tttttttaca ccccacttga cgattttcca
     9001 aatcagtatt aatttatata cagaaattag cactcactca tatagagtgc taacaacaaa
     9061 tcctgaagtt cataggaggt gtgctatgaa atggggttta acaaaacgag ataacaccgc
     9121 tctcaatcca tttgaagaat ttgagcgtga tgtcaaaaaa acctttgaaa acttcttttc
     9181 attagaaccc gctaatcttt ttgattctgc atggataccc acggtagatg ttaaagaaga
     9241 tgaaaagtct atccatgtgc gcgcagaaat tccgggaatt gatgaaaagg atatcaacgt
     9301 tgcattggaa gacaatatcc ttaccatcag cggggagaag aaagaggagc gtaaggaaga
     9361 gaataagcgt tatgttcttt ctgaaagtcg atatggttca ttcaaacgct gcattactct
     9421 tcctggcgag gtaaaagctg acagcgcaaa agccaaattt aaaaacggtg tattaaccat
     9481 tgactttgaa aagaaggaag tttcgcaacc aaaacgaata accattaatg ttaaataagg
     9541 aggcaggcta tgtttggaac atacgatctt tttgatgatg tatttgcatt aaggaatctg
     9601 gttgaccgct tctttgatga agctgcattt tcacggcagc gtgtggactt tccttatgta
     9661 aatatctatg aaaaggacga tgaaatactt gtcagggcga tagctccggg agtaaaggct
     9721 gaggatgtag atgttcaact aatagataat acacttacca ttgaaatgac ctgtaaggaa
     9781 gattatgcca accagccata tctacgcaaa gagcgagagt ttggcacttt ccgtaagtcc
     9841 atacaactgc cctatcaggt tgaccctaac aaggtagatg cgtcattgaa caatggcata
     9901 ttgacaatca agctgtccaa gcacgaagcg gtaaaacccc gtaaaataat gattaagtaa
     9961 taaaggaggt gaacagtatg gcaactcaat tagcacaaaa gcccgaacag aatgtannnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaag gaggtgaaca gtatggcaac
    10141 tcaattagca caaaagcccg aacagaatgt aaaaaagcat gattatgtga taacaccacc
    10201 ggttgatatc tatgaaaccg ataatgaata tgtattgaaa gccgacatgc ccggggttac
    10261 aaaggataat gtaaatatta cctttaacaa caacgagctt gaaataaccg gaaaagttga
    10321 tgagcagtac accagcaatg aaaacatgac gtatcgtgaa tatactctat tcaactatta
    10381 cagaaagttt gttgtatcta atgccataaa tcctgaagga ataagtgctt cacttgagaa
    10441 tggagtactt accgttacat taccaaagcg tgaagaagca aaacctaaaa agattgagat
    10501 aaaagctgag taaaaagtgt atactttacc cgtcgccggt gcgacgggta attgttttta
    10561 aagctgagta aaaaggtttt ttacttattt tctcaccagt gatacgatga attattttaa
    10621 agctgaatac ataggtttat tacctgtttt cccgacagta atacgatgcc atttttttaa
    10681 cgctgtataa aaaggttaat tacttatttt cccgacagcg atatgataga atgttcttta
    10741 agctgcatac ataagtttat cacttatttt cacgccagtg atacaatgga atgttcttta
    10801 agctcataca tggattttat tacctgtttt tccactgaga cgaggggaat gttttccatg
    10861 atttgtatat tctacttttt cccttctact gtaaaaagtg cctctatctt tttcccattt
    10921 tttgctgttg ctaccgctgt aacataagag gtaacagcaa taggttttac caccctcaca
    10981 gtaactgtat gtcctgatgc aggtgcaacc tgcaattcac tatcagcttt ccatgtaaca
    11041 gcaacacttg atggcagctt aaaccattga gcacctgtat ctacagtgct catgcctcgg
    11101 gcatatacaa caacaaactc cccaactcta agttttgcat gatcttttgc aactccgttc
    11161 atctgctttt catcggttgt caggtataat ccatcgagtc catagtattt aaatgctgga
    11221 gctgctggtt tttctgttat ttcagatgtt ccaacacagg ctaataacat gagaacaata
    11281 gataacgcta tcattgttac aatagtatgt tttttcatac ttaccccctt atataattta
    11341 ttagaatatt agtattaata tttttttata atgttacctt tgatttacca tattattttt
    11401 gcattaaccc acattgtcat agtataaagt ttataagtat aacaacggac agcttttaat
    11461 cagtatataa tctattatga tatcgtatta tttaattttc aaaagcatgg tatacaaaat
    11521 acaaaaacta acaagaaaaa atattaaaaa atttctttac gataatgcgc cagcatccaa
    11581 agacactctg caccactgtc tggctgttta ttgctgtgat gacatactgt cattctgaag
    11641 gagcgccagt ggctgaagat cctattattt tcttcaacgt ctggattctt tggctacacc
    11701 tcagaataac aaacaatact attactcaac tatggtaacc ttatattctt tgctcttgac
    11761 cggtttttta tttttatcaa atgcccttat gtactcaaat actatcgtag cagtacccgg
    11821 cttaaccgcc ttaaaggtaa aaagctcctt atctataccg ccaacaactt gctgttgcat
    11881 tgtttctacc ttataatcac tattgacaag cacaacagca ttttgctcaa ttttaaaaaa
    11941 ccatcggtga ccggtgctaa gctgggattc cagctcaaat gaaatacagc cgccattgtt
    12001 aataactatc tcctcacctt tgtaatgtac agcatttatc gttgtgcatg gttccttcat
    12061 atcttttgta caaccagcaa ccattatcaa tacaataaca tatagtaatc ctgatttctt
    12121 tatattaaca accattgcag tgtcccccaa aaataaaatt attctttatc cagtacaacc
    12181 caggctgcac cataaccaac attattacac ccatattgaa tccacatata acccttttca
    12241 ccccagcgct gtccccatga atttttaatg cgatatgcat gtttactatc atcccatcca
    12301 actatgataa tgccatggtt aacatcttta tcgctgctta ccggagtaaa ttcatcaaat
    12361 ataccgccaa catatgcctg aaatgccgga gtaaccttaa cggtggctgc tattggccca
    12421 taggtacata atgccttctt catctgctct actgttggaa tacctgcatc cgcacgcaca
    12481 tacccccatg caaccatttt tatgggaaac tgttttttag gtgtacatcc atattcatac
    12541 cccctgtatg gagcaacctt ttcttcaaca gggtaattat tcatcaaaaa atcaaaaaca
    12601 ttgccatacc agccgccatt acaactgccg gcatctctgc catatttatc acgagcacaa
    12661 tcaatgcaat actgctcgga taaatcaatt tcatcttttg cttttatctt gtagcacccc
    12721 tccacaacac ccatcgcggt aaatgaccag caactgccgc aaagcccctg atcctttatt
    12781 ggtgtcatca tccccaatgc aaaccagtcg agcgctggct ttgatggatc ggcaagcata
    12841 aatgactcgg ggagtgtttc ccgcggcttt tcaggttcaa ctgctttggg ctcttctttt
    12901 tttgcttctt caggttttac tggttctttt ttaggtggaa gtgttgcctc tttttgcttc
    12961 ttagcctttg attcagacaa acgttgcaaa aattccagga aaagctgtaa tccactatta
    13021 gtctgaattt cagcttcttt ttcaagatct tcaggtggaa tagctccggt aatttcatct
    13081 atctgccgct ttaatgcttc agtcatatca actttaaatt taagatttcg ctcttttata
    13141 tcttttttaa ttatggctaa ttgctgtttt acctcagcaa tacttttcgc atccctctgc
    13201 ttctgagcct caacactggc tttaaatgaa cttttcattt gtgcatgaga aaaagcaaca
    13261 ggagctatca acatacaaac agtaatactt gctgcaatgt gctttttcat atttattatt
    13321 cccccctgaa attgaatatt gtgttattcc gaggaagcgt tagcgaccga agaatctatt
    13381 cattatatat tcattcataa aggagattct tcgctatcac tcagaatggc tatcggcttt
    13441 gcctcagaat gacaataacg ctacagcttc aacccgcgtt tggtgatcct gttaaaccac
    13501 caatggctat acgcggccat cagcaactgc actataatta cacaatgtta ttatcaaata
    13561 tactgaacca aatatataat gatctatagc aaatgtctat tccttttttt attgaaaaat
    13621 atttaaattt ttgatatacc ataaccgata aaaaagtgta atagcataat gtaagcataa
    13681 cgtgcatctt atcataagag ggtatccact atgaaatcaa tacgattttt atttactgtc
    13741 accatcactg tctttgttct tgcagcggtt tcaggtatcg tcattgcaca acaacaagct
    13801 gcaccaaaag aaccacaaaa agaaggcata aaagaagaag ttaccggctg gcgcatcaaa
    13861 gattttaaac cctatataaa agcgatagaa gagcttgaaa aactcagcaa ggaatattct
    13921 gataatcttc ttaaacttgc aatagatgaa tattcaacgg gtatagatat acttgaagac
    13981 atggaaaatg aggttaataa attagtagaa acaaatcaaa agaataaaaa cctcaatgag
    14041 cagtggtact ggcaggaaat agacaggcgc gaacaggaaa agcgtcagat atcaatgatg
    14101 aagtttgaag caaagagcaa agcaataacc tactttacac gcgctatcaa ccatatggat
    14161 gaaataacct ttgaggaagt aaggaaagac ccaaaatttg ttaatttcca gattggatta
    14221 tataaggcat atgtatccac gcaatatgat ctttacaatt taaaaccatg tatccctatc
    14281 ctggagcgct acattgctat taacgaccaa actaaaaacg atgtatgggc atataagtat
    14341 cttgcaagct gttatggata catggaaagt gttctggtaa aatacaaaca gggtacagag
    14401 caggagcagt tgcaatataa gcaaaagaag aatatatata tgctcaaagc tgccgaatta
    14461 caatacggta ttgagtcgcc ccagtacaag catttacgtg agatagtatc tcaggatgaa
    14521 aagaaaagtg aaaagataaa tgaatttaaa tagcattaat ttttagcaat tcttccacaa
    14581 taaaaaaagc aggctataaa agcctgcttt ttttattgct ttctactttt gtgactctac
    14641 tttaaaatga gaatgaacct gatattctgt accattgaat atcattgtaa atacaataac
    14701 cctgccggga tacacatata ctgtaagagg tttcggctgg cgtatgttat tgtcacgtat
    14761 gtatttattc tcagtttcat caagacgcca gcgttcaata attatttcat ggtactgaca
    14821 tgcagtcaaa tttaaatcaa ttgctgtgct gttaatagat aatccgggct cagtcctggt
    14881 atagtccacg ccatcgcaca ttattttata aaacactctg ccagcctttt cttctgatgg
    14941 catatcgtag agtgctatct caacagtatg gtcttcactg ctgacatact gcacattgct
    15001 ggcatacctg tatacaggct tttgggcaac aacatcaatt acctcggttg catcagtatc
    15061 ggcaaccagc acaatgcggc tatctatcgc ccttttaaaa tatggtcttg acaaaaagta
    15121 aagcggcagc ttaccatcat tatattcaag ataattatcg tttgtaagca ccgccaatcc
    15181 gccgtccagc agcacaaaat cggtaatagc ttctttttgc tgtacaatac cgatgttaac
    15241 atcggcgcga taaagatagc agtttgtaga atcatgaatg ctgatataca ctaccggtgg
    15301 gtcaaatgct atacgcttta cctctgatga ttcgttgatg ctggctacaa cacatacaat
    15361 agattttttt tcatcaagaa gatatgcacc agcggtaact tctttttcac ttcttgccac
    15421 atacaaaatt gctctattgt ttattgccgt aaaatccagc actgttccat taacaggaag
    15481 cgaagtattg ctatcaagat aaataacccc attgtgtaaa gcaaaagctt cacacgcatt
    15541 accctgcaaa caaaagcaaa agtggaaaat aaaaagaagc atccagaaaa tacaattaaa
    15601 tgtggaacaa cctgttttta aaatcatgat atattgttaa cccaagctta ttgtgctcgt
    15661 caataatttc ctgtaatatt gccggcgttg taagcggatt tatggtaata gctcgcagta
    15721 caacaatctt ttgccctcta tagcgtgttg actccagcat ggtgcgggaa acaaagctat
    15781 tatcttcctg gcgtattgct ctatgcagtt caatattcat ttcattgata ccatcgttga
    15841 gtttctttaa acgcttttgt aaccgtgttg ctttttgctg gtcggcattg ctcaattgtt
    15901 cataaattct gttaattttg ctttgaacac cttcaggaac aaatcggtaa ttggtgataa
    15961 ataattcagg gctgttgagc aattcaaaat tttcatgttt attgatgata tctttcaacg
    16021 tactggtaag cataaaggca tgatcaaaca acacctcaaa acctgtggtt ccaaatatct
    16081 ttaaggtaac ccatggcttt aaacatgaaa atgggcgtga tccctctaca gtaaacctcc
    16141 cctgatcaac agaatcggga cgtatgatat aatctgatgt atgcatgata tttagaagat
    16201 catagggatt tttaaacaat atcaatccca tggacaatgg cgaatataac aacttgtgag
    16261 catcaaatgt taccgaatcg gctttttcta tacccctgaa aagatcgcga tacttgttta
    16321 ccagtaatac tgaccctccc cacgcagcat caacatgaaa aaatatgttg tattcatcgg
    16381 caatcttccg taaggccata agatcatcaa tgttgcctgt ttcggtagtt ccagcaatac
    16441 caatcaacga tatgactttt attgtatcat gatggctgct attatattcg gatatctctt
    16501 ttaatacttt ttttagtgca ttaatatcaa ttttattgtt actatcgaca ggaattttta
    16561 taacattatt gtcccctatc cctgtgatgc gggcaacttt atctattgag taatgtccgc
    16621 gctgcgaaac aatgaacaca gctttatgaa tggcatagta gcgaaaagtt tcagccacac
    16681 cagctttgcg tataccggga aaacgctcat ctgccggaaa cgctttatta cgtgcaacca
    16741 gcatagcggt aagattggcc aatgtaccgt caagagtaac atttcccagc gcaattctgt
    16801 gattttgaat atttcgttta taaaatgaag ctgatttgca gaaaataagc cggtgaatcc
    16861 atgaaataaa ttcacgttcc acaaatgttg atgcctttgc tgtttcaatt tttacctggt
    16921 tttgatttaa tgctgcaatg atcatctcaa gtaaaatcat aaaataagga atagcactgg
    16981 tcatgtgccc tatataataa gggttgccaa ccttcaccga atgtttaatg accttttgct
    17041 ttacctctgc cagtatatct ttcaacagtt ttggctcttt agggatatcg atagcattaa
    17101 acaaacaggc taactggggt aatgaaattt cgctgtggat gccccccttt tccttaaaaa
    17161 aggaatgaat caaatcaaga agctcatagc caaattgcat aaatctatcg ggcgagtcgg
    17221 gcattataaa aagcttgcgc agatgctcta aatcggtggg aatcttactt tgaacatgct
    17281 ctattgaagg ctcagccaat ctcatcggga taaccattgc tcataaggtt ggtgatgact
    17341 atgttactac aaaactatag ttaaaaaatt aatcaagtac ttttgtttag aatatacaaa
    17401 tttattgctg atacctataa caatcatcca ccagaacaat acgtaccggg acattatgcc
    17461 gtataatcga cccgctaaaa atatacctcc ttactataaa ataactaatt tttattattg
    17521 tataatgttc gatagttact gtataacact gtacacaagc tgtgaaataa taagggtatc
    17581 atataaaaaa tagtatcctg acggcttttg ttcgatagtt actgtaaatg ctggtatatt
    17641 ttattgaagt gaagtgaagt gaagtgaagt gtaaggagtt tgcgatggct ggcttgttgc
    17701 tatccggatg tcatgaaaat atttttcggc agtcatcatg aaccctgatt caggatatct
    17761 gaaaattatc gggattccgg ggcaagcccg gaatgacatt ggcgcataat tctattttta
    17821 aggagctatc gcaatggcaa ttcttaaact tgaaaatgta tctttgagct taaacaataa
    17881 accaatatta gacagcattt cggtagattt ctgggaagga tatatccatg ccattgttgg
    17941 tcctaatggc gccggtaaat caacgctatc ctatgtcata atggggctta acgattatcg
    18001 caccatcagc ggcacattgt attttaaaaa taaaccaatc aatgaactat cggtatacca
    18061 gcgggcacgc cttggcataa gccttgcatg gcaggaaccg gcacgctttg aaggattaac
    18121 cgtttatcag tatttacagg cttcaaaaaa ggataatgga ttatcaatag aagaagcaat
    18181 ggatgttatt ggtttgccag ctcaggaata tctgccgcga gcatgtgaca aaacactaag
    18241 cggtggtgag cgcaaacgca tagagctggc gtcactgctt gtgatgcagc cggaggttgt
    18301 tttgctggac gagcctgact caggaattga tgttgaagca ctggagcgga tattcgatgc
    18361 aatggcaatt ttaaagcaac gaggctctac cataataact atcacccata gtatggccgt
    18421 tttgcaaaaa gcagaacatg catttttgat gtgccatggc aatatcatcg ataaaggtgg
    18481 cattgataaa atttcaggat attttgaaaa caaatgcatc ccctgcgatc ataaaaatat
    18541 acctatttct ggagaagcac gctaatggat gatagacgag cagttgcaca aaccttatac
    18601 aaaaccatgg gattaaccga cgctgcactc aatgaccctg atattgcgca tctggttatc
    18661 aatagcaata tggttgttgc ttcacacctt gttgaagggc ttgttgttga gccggctgaa
    18721 acacaaaacg gcgtgagggt aacaatgagg gtgctggcgg ggcatataat acaaaagccg
    18781 gtacaccttt gttttggcat gctgcctgaa gatggactgc aggaaattat tatggacgtt
    18841 accattgaag agcatgccag tgtttccata cttgcccact gtacattccc caatgcaaaa
    18901 aatataaccc acaagatgga ggctgttatc accattaagg aaggtgccag ctactcgtat
    18961 cttgaaaggc atgtccatgc agccagcggt tttattaccg tgcttccgca tgcgatagtt
    19021 actgttgaaa aaaatgctac atttaaaacc gactttgaat tgttgagggg taaagccggc
    19081 attattgata ttgattacga aaccacctgc tatgagcata gccatttaga gatgaatgca
    19141 aaggtaaacg gcatacagga tgatgcaatc accatccgcg aaacaggaca tcttaacggt
    19201 gaaggtgctg tcggtgtact taccacccgc attgccgttc gtgatactgc acaggctact
    19261 gtgtacaata ctttaactgc acacgcagcg tacgcgcgcg ggcatgttga ctgtaaagag
    19321 attatacagg atagggcaac tgctaaagct attccggttg ttgaggtata tcatcctaag
    19381 gcacatgtta cccacgaggc tgccattggc agtgttgatg ataaacagtt gcaaacgctc
    19441 atggcccgtg gactttctga ggatgaggcg gttgatttaa tcatacaggg tttgttatca
    19501 gggagttaaa tccttatctt tccttcatcc tattttttga agtcttctca atgacaagag
    19561 ctatcgaccc gagtagtgaa agcaaaacaa aaaacaccat tgtttttatt gataaatcac
    19621 tgccctgttt tctgcgctca agtaaaagct caagatacat tgtatcgttg acatcaaaat
    19681 cttttttttc agcaactcgc aaatcaaaat cttttaatat atactcaatg cctgcaccaa
    19741 cctcatcata acggttatag ccagcaaaga tgtggggttt gcgaagcatg atgtatgcct
    19801 gttttaattg ctgtgacgac ggattatcat ggtagataag cgattcaatt gcgggctcag
    19861 taataaaagt tgaatacagc ccgggcaatc caaacaacag tgcaacaata aacatcgcaa
    19921 tggtaaaata agcataaatc tttgtatcat caaatgtcat ttttccacct ctttaagata
    19981 cgtaacaata tctacagttt tataggtaac attatcaacc cgtaccatcc ttgcaggtaa
    20041 cacaagagca gcttcacgct gttttattaa ttcatcgacg ctaatctcca gagcatctaa
    20101 taataccttg atagtatcct cgctatcttt ttgttgaaca aacaactgtt tttgctgctt
    20161 tggcaaaatt tgctctgctg ttacggtaca ttgttttact gctttttgtt cttcatcaag
    20221 tattgataca acagcactat agctggtaat caccgtatca acacttttat tattgatata
    20281 tattgtgtat gttgatgcaa acactggaac attcttttca aatgtaacat taacagtaaa
    20341 ttccacatcc cccagctcaa catagcgcgt gcagcttaaa cacaccacac ccacgcaaat
    20401 gattaatatc ttcttcatta catccctcat taatgttatt gtgcagttat gaaataatta
    20461 tgaatattta gtcaagtaaa atgtatggat agttacagtt attccatagt tattttgcag
    20521 caatagtgca gtgttaataa aaatgcaagc tcaagattta tctttaacaa ctgtatactg
    20581 caaaaattct tattgagact gtattcttat ttacaataaa aacatacata ttaactggaa
    20641 atctaaattt tagcaacgaa ggtacgtttt ataagggttt taaatatact ttaatcttct
    20701 agcattaaat gcatttaacg taccttctaa tgtgtcagaa gcatgtttat taattgcaga
    20761 attttcaatg gatccgatct caaatgtaaa tttattattg tgatctttat ctgttttttc
    20821 atttaattgt ttttttatta tctcaccact tttccatatt aattcagctt taaaatttga
    20881 tgccagatca tctctcaaag acctatctgc ttttagaaag aaaatcacgg taattatgct
    20941 aattcccgca taaacttatt atgtctgtta atgcactttt accatttcct ttatttccaa
    21001 taatagctac cagcccataa ttcaagggaa tttcaatatc tttaaaccaa attccttgtt
    21061 cttcattata tccgttcatt tgatttattt tcaactgctt aataaatttt gttttgtttt
    21121 ctttaacttt ttttaatgca tcaggttctt ctcttacata aactcttgcc tctggttcaa
    21181 agattatttg ccttaatcct ttaaatgttg ggtctgcttt aatccatgtg taacgtgaaa
    21241 ataaattttc tatagcatga gcatcagaac caataataca aggttttaag gatttaaatt
    21301 cttttgtgaa ttttttttct ttttcttcta ttgtttcacc ttcgctttta tgccctaatg
    21361 cccaatttat ggtattttca tttgtagaaa ataagatatc agctttttaa ataaaaattt
    21421 tccttgttaa atgattctgc ccattccaat ttaaattaga taaatcttca tcagcaggta
    21481 caagtgtgag atatttattt tcaaataaag actttttatt agaaaggata cttaaaatct
    21541 ctgtatcatc tacaacagca ttcatacaac caatgtataa atctgagtca tctttaaaat
    21601 tcttatgctc gctttttaat ttttcatcga gttgtattag attatgcttt gttaattttc
    21661 ttttctcatc ttcattttga ggattacttt cataaacaaa atttatttct tgtaaaaatt
    21721 ttctttaatt tcttttattt caatttcatc agagaaaata acatgaaagt ttatcctatt
    21781 cttaccgacc aatttattta atcgaaattc tatatttgga agaatcaaaa ttctttttac
    21841 ttgttcaatt ttttcatcaa ataatttttt taatttgtta tcgttgctaa tataatcatt
    21901 ttttattttt catatccgtt tattatgaaa taccaatagc ttcaattgta ttttcaattg
    21961 ccttatcgaa tacgttttta acataattat cccacgtttc ttcagattct ggatcaacaa
    22021 attgattatt taatactgaa tatgctgtat aaacgtgtaa atctcatctt ctccaaattg
    22081 aactctatgt attcatagtt taatctttat ttttaattta aacattttta agcactacct
    22141 tacctcagca tctctcaaat gcttaatcgc aatttttata tatctgtcat cagagaatct
    22201 ggttcttttt ctttcgctcc atttgttgag ttcttgctta atgacatcta tatcattggt
    22261 attttttttg ttca
//
LOCUS       DAOOJH010000032        21975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2403_length_21975_cov_8.874863, whole genome shotgun sequence.
ACCESSION   DAOOJH010000032 DAOOJH010000000
VERSION     DAOOJH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21975
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_21975_cov_8.874863"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..516
                     /gene="rpsL"
                     /locus_tag="PLJ75_06020"
     CDS             142..516
                     /gene="rpsL"
                     /locus_tag="PLJ75_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPD04809.1"
                     /translation="MPTINQLIRKGRSEKVKKTKSPALKSCPQKRGVCTRVMTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGIGHNLQEHSAVLVRGGRVKDLPGVRYHIVRGTL
                     DSLGVNDRKKGRSKYGTKRPKA"
     gene            609..1079
                     /gene="rpsG"
                     /locus_tag="PLJ75_06025"
     CDS             609..1079
                     /gene="rpsG"
                     /locus_tag="PLJ75_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPD04810.1"
                     /translation="MARRRRPAKRTILPDPKYGNIVVSKFINSMMTEGKKGVAERAFY
                     KALELIGQRTQKDPIEVFINSLENVKPLVEVKSRRVGGATYQVPVEIRPERRQALAIR
                     WIVTHSRNRSEKTMFQKLAGELLDAFNNTGTSIKKKEDTHKMAEANKAFAHYKW"
     gene            1203..3281
                     /gene="fusA"
                     /locus_tag="PLJ75_06030"
     CDS             1203..3281
                     /gene="fusA"
                     /locus_tag="PLJ75_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD04811.1"
                     /translation="MGRELPIHRTRNIGIMAHIDAGKTTLTERILYYTGKTYKIGEVH
                     DGTAEMDWMIQEKERGITITAAATTTYWKNTRINIIDTPGHVDFTVEVERSLRVLDGA
                     IAVFDAVAGVEPQSETVWRQADHYNVPRICFINKMDRVGADFERCLTMIKERLYSIPL
                     PLQIPYGKEDNFRGVIDIVQMKGVIWTDASGEHYEYTAIPDELQGEAQYYREHLIETV
                     AEHDEQILDKYLAGVEIDEQELKRAIRTVTIKSALFPVFCGSALKNKGVQPLIDAIVD
                     YLPSPKDIPSIKGNDAHHDDVIVERHPDDKEPFCALCFKIMTDAYVGKLCYVRVYSGH
                     VSVGESILNVNKQKKERIQRILRMHANDREDIKELYTGDIAAIVGFKFAQTGDTITSI
                     DAPIVLGKMEFPEPVISIAIEPKTRADLERLHMILKRLVDEDPTIFVKVDEESGQTVL
                     SGMGELHLEIIVDRLQREFNLPVNIGKPQVAYKETVSTTAKAEGIFEKEIGGKLQYGH
                     VLLEVSPCDSHFEFISKLDSAAIPDIIIKAIEMGCRDALNSGVIAGYEMTRIRVIVED
                     AKYREDVSVEHAYRIATTMAFRDAVKKATPVLLEPIMKLEAVVPDEYMGDIINDINAR
                     RGKIEHIDIRGMLKVIDAYVPLSEAFGYATAVRSLSQGRASHTLQFNHYDVVPQNVME
                     KIIGSYYRPV"
     gene            3576..4790
                     /gene="tuf"
                     /locus_tag="PLJ75_06035"
     CDS             3576..4790
                     /gene="tuf"
                     /locus_tag="PLJ75_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016209759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPD04812.1"
                     /translation="MAKAKFERTKPHVNVGTIGHIDHGKTTLTSAITKVLTAAYGGHN
                     KPIDFDQIDNAPEEKARGITIATSHQEYETPNRHYAHVDCPGHADYIKNMITGAAQMD
                     VAILVVAATDGPMPQTKEHVLLARQVNVPYIIVFLNKVDMLDPADRDELVELVEMEIR
                     DLLNKYEFPGDTIPIIPGSALKAMQCGCGKADCEACGPILKLAETLDTYVPEPKRDKD
                     KPFLMPIEDVFSITGRGTVVTGRIERGMVRTGDEVEIVGFGETKKSVVTGVEMFRKIL
                     DEGMAGDNVGCLLRGIGKEEVERGQVLAKPGSITPHRKFEAEVYVLSKEEGGRHTPFF
                     TNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIKMTVELITPVAMEETLRFAIREGGR
                     TVGAGVVAKILE"
     gene            4969..5286
                     /gene="rpsJ"
                     /locus_tag="PLJ75_06040"
     CDS             4969..5286
                     /gene="rpsJ"
                     /locus_tag="PLJ75_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPD04813.1"
                     /translation="MAKLAGQRIRVKLRSFDPYLLDQSAAKIVATTNRSGAKVAGPIP
                     LPTRIEKFCVLRSPHVNKKSREQFEIRTHKRLIDIIDPNSDTVEALMKLELPAGVSVD
                     IKS"
     gene            5369..5986
                     /gene="rplC"
                     /locus_tag="PLJ75_06045"
     CDS             5369..5986
                     /gene="rplC"
                     /locus_tag="PLJ75_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPD04814.1"
                     /translation="MKKALIGRKIGMSQLFLDDGTVIPVTVCELGPCVVVQKKTIEKD
                     GYNALKLGYWETKPHRLTKPIIEDLKKKNLPIVKVFKEVDIFDENLVEGSVITCEIFS
                     ENQIVDVTGTSKGKGFAGVIKRHGFHGGKETHGSNFHRAPGSIGANTYPAEVWKGKKL
                     PGRHGGKTVTVKNLKVIRIMKDKNLVIISGAIPGTVNSIITVQSK"
     gene            5998..6624
                     /gene="rplD"
                     /locus_tag="PLJ75_06050"
     CDS             5998..6624
                     /gene="rplD"
                     /locus_tag="PLJ75_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPD04815.1"
                     /translation="MVVDVYTLEGNIKGQIELNESIFNAKVNDVLMYELIKAANANLR
                     QGTHDTKERSYVSGGGAKPWRQKGTGRARQGSIRAPQWKGGGAVFGPHPRDYRIELPK
                     RIKQEAYRSLLSMKFKEGSIKIIEDFKVVDGKTKEVAAIAKKLNISKGVLITDIEDNL
                     LKRAIRNIPWFLYNNVNRLSGRDIFYTKTVLITENAAKIINSKYAKGE"
     gene            6627..6911
                     /locus_tag="PLJ75_06055"
     CDS             6627..6911
                     /locus_tag="PLJ75_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006720287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPD04816.1"
                     /translation="MNANDIIIRPVITEKATEMAKQNKYIFRVHKKANKDMIGRAINN
                     IFGVMPVRINVMIVRGKRKRVRYNYGYTASWKKAIVTLKEGEKIDVFETK"
     gene            6945..7769
                     /gene="rplB"
                     /locus_tag="PLJ75_06060"
     CDS             6945..7769
                     /gene="rplB"
                     /locus_tag="PLJ75_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPD04817.1"
                     /translation="MGLKKYRPITPTLRYQTVLDYSEITTDEPLKSLTKGKKGYAGRG
                     FKGQISVRRKGGGHKRRFRLIDFKRNKYNIEGKVSTVEYDPNRSANIALIIYKDGEKR
                     YIVAPFSLKVGDTVVSGDNVEVKPGNAMPLKNIPDGTSIYNIELHKGKGGQLVRSAGV
                     SAVITAKEGEYCLIKLPSGEIRKIYCECYATIGEVSNKDHINIDIGKAGRNRWMGKRP
                     KVRGVAMNPIDHPLGGGEGKSSGGRHPCSPTGKPTKGYKTRKKKKYSDKMIIKRRK"
     gene            7773..8060
                     /gene="rpsS"
                     /locus_tag="PLJ75_06065"
     CDS             7773..8060
                     /gene="rpsS"
                     /locus_tag="PLJ75_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPD04818.1"
                     /translation="MARSLKKGPYVDIKLFKKIEEMNSTNAKKAIKTWSRRSTIFPEM
                     IGHTIMVHNGKNFIPVYVTENMVGHKLGEFAPTRTFRGHAGKDDKVAKRGK"
     gene            8061..8399
                     /gene="rplV"
                     /locus_tag="PLJ75_06070"
     CDS             8061..8399
                     /gene="rplV"
                     /locus_tag="PLJ75_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPD04819.1"
                     /translation="METKAVSRYNRISAVKARLVANEIRGFSFPEAIDILRAIPRKAS
                     KLLMKTLYSAAAGAKYRNPEIRENNLYIKKIVIDEGPTMKRFRPRARGRASRIRKRLS
                     TIEIVLSDEN"
     gene            8420..9100
                     /gene="rpsC"
                     /locus_tag="PLJ75_06075"
     CDS             8420..9100
                     /gene="rpsC"
                     /locus_tag="PLJ75_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPD04820.1"
                     /translation="MGQKVNPTGVRVGIIRTWDSVWFSEKDEYRKNLHEDIQVRRYIM
                     KKFKNAGIAKVGIERFPDRVNVYIHTARPGLLIGRKGVDIENLKVDLQKKINKNVNIY
                     VQEVKKPEKISKLVAEQVASQIENRFPYRRAIKQAITAAMRSGALGIKVSISGRLNGA
                     EMARYESYKEGRIPLHTLRADIDYGFAEALTTFGLIGIKVWIYSGDVLSNAGEQEEDK
                     FALRKQSR"
     gene            9120..9536
                     /gene="rplP"
                     /locus_tag="PLJ75_06080"
     CDS             9120..9536
                     /gene="rplP"
                     /locus_tag="PLJ75_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPD04821.1"
                     /translation="MLMPNRTRYRKVHRGRMKGKATRGSTIHFGEFALKTIECGEINS
                     RQIEAARIAISRKVKRGGKLWIRIFPDRPFTKKPAETRMGKGKGNPEGFVARIKPGRI
                     LFEISGIDDAMAREALSLAAHKLPVKTKIISIHDNN"
     gene            9552..9761
                     /gene="rpmC"
                     /locus_tag="PLJ75_06085"
     CDS             9552..9761
                     /gene="rpmC"
                     /locus_tag="PLJ75_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPD04822.1"
                     /translation="MKGKLDELTVEELNRSLREAKEELRKERFKRTVSRVENPKKTMQ
                     LKKQIARILTVLREYESGTRTARSK"
     gene            9779..10033
                     /gene="rpsQ"
                     /locus_tag="PLJ75_06090"
     CDS             9779..10033
                     /gene="rpsQ"
                     /locus_tag="PLJ75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPD04823.1"
                     /translation="MQENSRKQIVGKVISNKMNKTVVVEIERMMMHPKYHKYVRRTKK
                     VKSHDEKNECSIGDIVLIEETRPLSKEKRYRLVKIVEKVK"
     gene            10044..10436
                     /gene="rplN"
                     /locus_tag="PLJ75_06095"
     CDS             10044..10436
                     /gene="rplN"
                     /locus_tag="PLJ75_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPD04824.1"
                     /translation="MIQVQTMLEVADNSGVKRVQCIKILGGTRRRYASVGDVIIVAVK
                     DAHPSYGLKDSTGKKVHTKAVLRAVVVRTTKPVRRSDGSYIRFDDNAVAIIDNKGEPR
                     GSRIFGPVARELRDRNFMKIISLAPEVL"
     gene            10449..10766
                     /gene="rplX"
                     /locus_tag="PLJ75_06100"
     CDS             10449..10766
                     /gene="rplX"
                     /locus_tag="PLJ75_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPD04825.1"
                     /translation="MIRTILKKNDMVMVITGADKGKRGKILFVDRKRGRVVVEGVNVR
                     KKFVRPSQDNPKGGQIELPYPINISNVMVFCDKCKKAVQLGMKIDGDTKYRICKKCGK
                     RID"
     gene            10778..11326
                     /gene="rplE"
                     /locus_tag="PLJ75_06105"
     CDS             10778..11326
                     /gene="rplE"
                     /locus_tag="PLJ75_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPD04826.1"
                     /translation="MAARLRMAYEKDVKKRMMDRFKYSSVMQVPKIEKIVLNVGEGEA
                     HSNPNLLQSIINELSLITGQKAVKTVARKSIAAFKIREGYDVGARVTLRGDRMYEFLD
                     RLINVALPRVRDFRGLSPNSFDNAGNYNFSVKEQIIFPELNLDKVEKIYGINITIKTT
                     ARNANEARALLEEFRFPFQEAR"
     gene            11333..11518
                     /locus_tag="PLJ75_06110"
     CDS             11333..11518
                     /locus_tag="PLJ75_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPD04827.1"
                     /translation="MASERMFAKARREPKFKIRYRNRCKICGRSRGYYRRFELCRICL
                     RKLAGEGLVPGVKKSSW"
     gene            11542..11943
                     /gene="rpsH"
                     /locus_tag="PLJ75_06115"
     CDS             11542..11943
                     /gene="rpsH"
                     /locus_tag="PLJ75_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPD04828.1"
                     /translation="MSCVSDPIADMLTRMRNAINAGHVKVDIPSSKLKMAIAKILKDE
                     GFIKNFKLIDDNKQKLLRIYLKYSADNQSAIIRLKRISSPGRRVYLKAENLHPVYNNM
                     GIIILSTSKGIITNKAAKQLNIGGEVLCEIL"
     gene            11957..12502
                     /gene="rplF"
                     /locus_tag="PLJ75_06120"
     CDS             11957..12502
                     /gene="rplF"
                     /locus_tag="PLJ75_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPD04829.1"
                     /translation="MSRIGKKPIVIPNGVSIDMQNNNLTVKGPLGVLQYQLQDVINIA
                     IENNTIAVSKKDEKSNDKKVNALWGLTRALINNMVIGVSSGFEKNLEIVGVGYRAIQK
                     NNDVEFQLGYSHTILFTPPPGTKVEVVEPTKIRVKGIDKQLVGQVSANIRKLRPPEPY
                     KGKGIRYKDEQILRKAGKTGK"
     gene            12537..12902
                     /gene="rplR"
                     /locus_tag="PLJ75_06125"
     CDS             12537..12902
                     /gene="rplR"
                     /locus_tag="PLJ75_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPD04830.1"
                     /translation="MNRLKLKKVRYMRRRMRVKAKVRSNMQRPRMCISRSNKNLFVQI
                     IDDGQGSTLVCASTLEKDFPVVKNRGNKEAAKALGTIIAQRAIAKNIKEVVFDRNGYL
                     YHGRIKEFADAARENGLEF"
     gene            12914..13429
                     /gene="rpsE"
                     /locus_tag="PLJ75_06130"
     CDS             12914..13429
                     /gene="rpsE"
                     /locus_tag="PLJ75_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008432520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPD04831.1"
                     /translation="MKITRKRIKPEGLELAEKIIIINRVAKVVKGGRRFSFNAMSVVG
                     DNHGHVGIGFGKANEVPDAIRKSVEDAKKNVYKINVNKNTIPHEVIGRFKSTRVVLKP
                     AAPGTGIIAGDSVKAVVEKAGIVDIMSKTLGSRNPVNVTKATINGLLQLRSLKEVSDL
                     REIILPKLWQS"
     gene            13441..13629
                     /gene="rpmD"
                     /locus_tag="PLJ75_06135"
     CDS             13441..13629
                     /gene="rpmD"
                     /locus_tag="PLJ75_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPD04832.1"
                     /translation="MAKKLLIKQTRSYISAKPKQKATLRALGLRKLNAERVHDNTAVI
                     RGMVDKVKHMVKVSEINE"
     gene            13647..14084
                     /gene="rplO"
                     /locus_tag="PLJ75_06140"
     CDS             13647..14084
                     /gene="rplO"
                     /locus_tag="PLJ75_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPD04833.1"
                     /translation="MQSTIKKPEYIKKSKRVGCGQGSGHGKTSCKGHKGQKARSGAHF
                     KPGFEGGQMPLQRRIPKRGFNNTKFKEEVQVVNINDITKLSVKEITPEVLYKEGLIKS
                     QFLPVKLLAVGDINDAIAVVVDAISTTAKDKIIKAGGSVTLRA"
     gene            14112..15458
                     /gene="secY"
                     /locus_tag="PLJ75_06145"
     CDS             14112..15458
                     /gene="secY"
                     /locus_tag="PLJ75_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPD04834.1"
                     /translation="MPNVISNIFKIPELRKRVLFTLFILVVYRIGAHIPIPGVNIEAL
                     SSYFEMAAKSRGSGFVDFFDLFAGGALKRFTIFALGIMPYISASIIMQLLTVVIPAIE
                     KLSKEGPEGYKKINQYTRYGTVLLCALQGFGLTMWMKSMTSPKGAVVPQDSGIGFVLV
                     AVLAITTGTMILMWLGEMITERGIGNGISLIIYAGIVVRAPSAFVDTMNRIISRSDPI
                     IALILIALFIGVIAASILLTLGVRKIPVQYGKRVVGRRMVQMASQAIPLRVNAAGVIP
                     IIFASSIILFPAQISNMLGATKYGIFTKMQYWLSPGHTAYMIIYSLLIIFFCYFYTAI
                     QFNPNDLAENLKKYGGFIPGIRPGQNTADYIMHILNRITLPGSIFLALIAIFPDFIIR
                     IWPEEVSPEMAYLFGGTSLLIIVGVALDTLKQIESQLLMRHYDGFFKKGRIKGRRY"
     gene            15506..16258
                     /gene="map"
                     /locus_tag="PLJ75_06150"
     CDS             15506..16258
                     /gene="map"
                     /locus_tag="PLJ75_06150"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPD04835.1"
                     /translation="MAIYIYNEDEIKRVEDACRITAAVLEELGKNVVEGISTFELDRV
                     ARDLIRKSNAIPAFLGYKGYPAAICTSVNEVVVHGIPHRNQILRKGDIVGIDIGVKFK
                     DFFGDAAKTFIVGKVNNKVKKLLEVTKEALELGILQCKDGNHLTDISHVIESHVVQWG
                     FQPVHEFVGHGIGRNMHEEPSIPNFGKPGKGPIIKTGMIFAIEPMINEGTYQVEILDD
                     GWTTITKDRKNSAHFEHTVAVVHGSPKILTMV"
     gene            16285..16503
                     /gene="infA"
                     /locus_tag="PLJ75_06155"
     CDS             16285..16503
                     /gene="infA"
                     /locus_tag="PLJ75_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPD04836.1"
                     /translation="MAKEEPIEVEGEVVEALPNAMFRVKLDNGHVVLAHISGKMRMHY
                     IRILPGDKVTVELSPYDLSRGRITYRAK"
     gene            16527..16655
                     /gene="rpmJ"
                     /locus_tag="PLJ75_06160"
     CDS             16527..16655
                     /gene="rpmJ"
                     /locus_tag="PLJ75_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPD04837.1"
                     /translation="MKVRVSVKKICSDCKVIKRHGVVRVICKNPRHKQRQKKRTAK"
     gene            16674..17048
                     /gene="rpsM"
                     /locus_tag="PLJ75_06165"
     CDS             16674..17048
                     /gene="rpsM"
                     /locus_tag="PLJ75_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPD04838.1"
                     /translation="MARIAGVDLPNNKRVEIALTYIYGIGLPSSLKILEEARVNPDTR
                     VKDLTDEEINQLRKIIEKSYKVEGDLRTQIAMDIKRLMDIGCYRGIRHRRGLPVRGQN
                     TKNNAHTRKGNRKVSVGGKKKK"
     gene            17072..17458
                     /gene="rpsK"
                     /locus_tag="PLJ75_06170"
     CDS             17072..17458
                     /gene="rpsK"
                     /locus_tag="PLJ75_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPD04839.1"
                     /translation="MKQKRTKKQEKKNIPVGKAYIQATYNNTIITITDMQGNVVSWAT
                     SGGEGFRGSRKSTPFAAQMAAKAAAQKAIDNANMRSIEVYVKGPGIGREAAIRSLYQA
                     GLNISKIKDITPIPHNGCRPRKRRRV"
     gene            17475..18104
                     /gene="rpsD"
                     /locus_tag="PLJ75_06175"
     CDS             17475..18104
                     /gene="rpsD"
                     /locus_tag="PLJ75_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPD04840.1"
                     /translation="MAKYTGPLCKLCRREMTPLMLKGRRCLTDKCAIKHKKYPPGPPK
                     KRRIKLSDYGVQLREKQKIKRSYMLLETQFKRVFEEATRLKGVTGENMLVLLERRLDN
                     VVYRMGFASSRLQSRQLVQHGHVIINGKRVDIPSYQVKVNDIVEIKEDFKNNAILEDA
                     IKLSKAVGSVPQWVEVDYEKRNGKIIRLPERKDIEPIFNEQAVVELYSK"
     assembly_gap    18201..18300
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18301..18536
                     /locus_tag="PLJ75_06180"
     CDS             <18301..18536
                     /locus_tag="PLJ75_06180"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HPD04841.1"
                     /translation="VDESHDKLKAILAKSIDELEFSVRAYNTLKSLELNTLEDVVHKT
                     EDELKKSKHFSEQVMAEIKKKLEEYHLSLGIKE"
     gene            18551..19009
                     /gene="rplQ"
                     /locus_tag="PLJ75_06185"
     CDS             18551..19009
                     /gene="rplQ"
                     /locus_tag="PLJ75_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPD04842.1"
                     /translation="MRHRNNVRILGRSHEHRKAMYANMVTSLFQHERIITTKQKGKEL
                     KRLAERLITRAKENLNLTENDNAKKLHNKREVMRLIHNEDVVKKLFDDIAPRFVTRPG
                     GYTRMYLLDRRQGDAAEMAIVELVVKTEKKQEEKKDKEKKNKEKKSNKTT"
     gene            complement(19051..>19455)
                     /locus_tag="PLJ75_06190"
     CDS             complement(19051..>19455)
                     /locus_tag="PLJ75_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04843.1"
                     /translation="PEEYHLFFDRETIERYLKNGYRKLQEAKNIYNNQDIIYLKTKSI
                     KKHDELEFIIKQYLAAIDFCRLAKQYGIEIHKMIKINQLDAIQSKYGLESNKLDPIFD
                     DRIPEEFKVDANDNLNLVHTIEKSRLEKKTTK"
     assembly_gap    19456..19555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19916..20791
                     /locus_tag="PLJ75_06195"
     CDS             19916..20791
                     /locus_tag="PLJ75_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HPD04844.1"
                     /translation="MEKEDSKKKISFRQKNATICPVCGYEFYREEMLTGGGRLIAGKL
                     TDELRRTYEKNEKWGVIYPLAYVVTVCPRCLYAAYPKDFSNLQSEDLQKIQATANARK
                     QSIEKFFGKLDFNIDRGLYHGAASYLLAMDCYSFRNKMVAPTFKMAISAIRAAWLFGD
                     LAKLEPDTPYKKISDFFYKKAYDLYFKVVDIMQTGAEPVDAAGNIGPDIDKNWGYDGI
                     LYMCAILTLKVGAKEPDLKKRIENFERTKRYLSKLFGSGKASKNKPGPLLDMTRDLYD
                     KMNEMLEQWKNETPV"
     gene            20767..21549
                     /gene="truA"
                     /locus_tag="PLJ75_06200"
     CDS             20767..21549
                     /gene="truA"
                     /locus_tag="PLJ75_06200"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPD04845.1"
                     /translation="MEERDSRLRYALIVQYDGTHYSGFQIQHNANTIQYELEKAIKIL
                     TREDIRIAAAGRTDAGVHALGQVIHFDLSKLIPLEKMCIGLNGILPADIAIKKAFNVD
                     SHFHARYSAVAREYSYVLYNHRYKSPFMHYRALWVREPINIAFFKEACQYCIGEKDFA
                     SFCKKCSATEGTIRFVEYITVDRIFNDFVVLTIKGNAFLHNMIRILVGTLLMLMNNNE
                     HPLKMKEILDRCDRNYSGETAPAYGLYFKKVYYPAVYDFLNV"
     gene            21719..>21975
                     /locus_tag="PLJ75_06205"
     CDS             21719..>21975
                     /locus_tag="PLJ75_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04846.1"
                     /translation="MKNNVLYGMLTILLVFIIAGNGYAQRKKGDYFLKPQIGMWFGPV
                     TPVFSTADDVDTDLWGGGFMRFNISSLPLKIGFDSSYEHFKS"
BASE COUNT         7548 a         3003 c         4910 g         6314 t
ORIGIN      
        1 cgaggaagaa atttctgtag agaagtaaat tttcttgaca attaaaaggg taaaattgat
       61 taatcacatt cagttaaaat atggaagtga gaaaattata atattatagt gaacactaat
      121 aaatgatgat aaggagatgg aatgcctaca attaatcaat taataaggaa agggagatca
      181 gaaaaagtaa aaaaaacaaa atctccggca ttaaaaagct gtccccagaa gcgtggtgtt
      241 tgtaccaggg tgatgaccgt aacacctaaa aaaccaaatt cagcgttacg aaaggttgca
      301 cgtgttcgac ttactaatgg gatagaggta acagcataca ttccgggtat aggtcacaat
      361 ttacaggaac actcggcagt tttagtccgt ggtggaaggg tgaaggattt acccggtgtc
      421 aggtatcata tagtacgtgg tacattggat tcattaggtg ttaatgacag gaagaagggt
      481 cgttccaaat atggcacaaa gaggcctaaa gcataaatga tatcgaaacg ttgttacttt
      541 gatggagaag taatgtttag agatgtccat acaagtgatt tgaaaatttg taatagcgag
      601 gtttaagaat ggcacgaagg cgtagaccag caaaaagaac tatcttaccg gatcccaaat
      661 atggaaatat agttgtttca aagtttatca actctatgat gacagaaggg aagaaaggtg
      721 ttgctgaaag agcattttat aaagcccttg aattgatagg acaacgcacc cagaaagatc
      781 ctattgaagt atttataaat tcactggaaa atgttaagcc gcttgtagag gttaaatcac
      841 gaagagtggg gggggctacg tatcaggtac cagttgaaat acgtcctgaa agaagacagg
      901 cattagctat tcgatggatt gttactcatt cacgcaaccg gagcgagaaa accatgttcc
      961 aaaaattagc cggtgagcta ttagatgctt ttaataatac tggtacatca atcaagaaaa
     1021 aagaagatac tcataaaatg gcagaagcaa ataaagcgtt tgcacattat aaatggtgat
     1081 atatttactt attcaggctt ttatagtata ataataaaaa aataccaggg gtagccccct
     1141 ggtatttggt gctttagctt aaaaataaat atttaaaata cattgatgat taagtgttaa
     1201 ttatgggaag agaactgcca atacacagga ccagaaatat tggcatcatg gcgcatattg
     1261 atgccgggaa aactacatta actgaacgta tactttacta taccggtaaa acctataaga
     1321 ttggtgaagt acatgacggg actgctgaaa tggactggat gatacaggaa aaagaacgtg
     1381 gcattactat aacagcagca gctaccacaa cctactggaa aaatacacga attaatatta
     1441 ttgatactcc aggtcatgta gattttactg tggaggttga gcgcagtttg cgagttttag
     1501 atggggctat tgctgtattt gatgcagtag ctggtgttga accgcaaagc gaaacggtat
     1561 ggcggcaggc tgaccattat aatgttccac gtatatgttt tattaataaa atggacaggg
     1621 taggagctga ttttgaacgc tgtcttacca tgataaaaga acgattgtat tccattcccc
     1681 ttccattgca gataccctat ggaaaagaag ataattttag gggtgtaatt gatatagtgc
     1741 aaatgaaagg tgttatatgg actgatgcat caggtgagca ctatgaatat accgctatcc
     1801 cggatgaatt gcaaggtgaa gcccaatact atcgtgagca cctcattgaa acagtagcgg
     1861 aacatgatga gcagattctt gataaatatc tggcaggagt tgaaatagat gaacaagagt
     1921 taaaaagagc tatccgcact gttacaataa aatcagcatt atttcctgtt ttttgtggga
     1981 gtgcattaaa gaataaggga gtgcaaccat tgattgatgc tattgtggat tacttaccct
     2041 cgcccaaaga catcccttca attaaaggga atgatgccca ccatgatgat gttatagtgg
     2101 aacgccatcc cgatgataag gaaccatttt gtgctctttg ttttaaaatc atgactgatg
     2161 cgtatgtggg aaaattatgc tatgtacgtg tttattccgg gcatgtatcg gtaggtgaat
     2221 ccattctaaa tgtcaacaaa caaaaaaagg aaagaataca acgcatatta cgaatgcatg
     2281 caaatgacag agaagatatt aaggagctat atactggtga tatagcggcg attgttggtt
     2341 ttaaatttgc tcaaactggt gatactatta cttcaattga cgcaccgata gtattaggaa
     2401 aaatggaatt ccccgaaccg gtgatttcta tagccattga acccaaaaca agagctgatc
     2461 tagaaaggtt gcatatgata ttaaaacgcc ttgttgatga ggaccctacc atttttgtta
     2521 aagttgatga agaaagtggg cagactgttc tttcaggaat gggtgagctt cacctagaga
     2581 ttatagttga tagattgcaa agggaattta atcttccagt taatataggg aaaccacagg
     2641 ttgcctataa agagacagtt tctacaactg caaaagctga gggtattttt gaaaaggaaa
     2701 ttggaggtaa attacagtat ggacacgttt tgctggaggt tagtccatgc gatagtcact
     2761 ttgaatttat atcaaaatta gatagtgcgg ctataccaga tataattatt aaagcaatcg
     2821 aaatgggatg cagggatgcg cttaattccg gtgttattgc aggatatgaa atgaccagaa
     2881 tacgggtgat agtggaagat gctaaatacc gtgaagatgt atcagtagag catgcataca
     2941 ggattgctac gacaatggcg tttagagatg ctgtaaaaaa ggcaactcct gtgttgttag
     3001 agccaattat gaaactggag gcggtagttc ctgacgaata tatgggtgat attatcaatg
     3061 atatcaatgc acgaaggggg aagatagaac atatcgacat acgaggtatg ttgaaagtta
     3121 ttgatgctta tgtaccgcta tcagaggcat ttgggtatgc tacggcagta cgttcgttga
     3181 gtcagggtag ggcatcacac acactgcagt ttaaccatta tgatgtagtg ccacaaaatg
     3241 ttatggagaa aataataggg agttactata gacctgttta atgaatctga tattataaat
     3301 ggtattaatt aatttttatt ttttttaaaa aatatttgat atttttaaaa ttacgcaata
     3361 aattagttga cagatatagc atatattttt taattcacac acttccacct tttgtccctc
     3421 aatagatgat gtgggagatt gtgttatata gagtgaatac attcttgtaa ttgaagattg
     3481 ttaaaatttt attatatcta tagtaaagta gagtatgctt aatctgtttt tttttgctaa
     3541 gttgaaaatt attattttga tgaaggagat aacaaatggc aaaggcaaaa tttgaacgaa
     3601 caaaaccgca tgtcaatgtt ggtactattg gtcatatcga tcatggtaaa acaacattaa
     3661 cctctgcaat tacaaaggtt ttgacagcag cgtatggcgg tcataataaa cctattgatt
     3721 ttgatcagat agacaatgca cctgaagaaa aagcaagagg tataacgatt gccacgtcgc
     3781 atcaggaata tgaaactcct aataggcatt atgcgcatgt tgactgccct ggccatgctg
     3841 actatatcaa aaatatgata accggtgctg cccagatgga tgttgcaata ttagttgttg
     3901 ctgcgacgga cggcccaatg ccacagacaa aagaacatgt cttattggca cgacaggtaa
     3961 atgttcctta tattattgtt ttcttgaata aagttgacat gcttgatccg gctgatagag
     4021 acgagcttgt tgaattggtt gaaatggaga tccgtgattt actgaataaa tatgagtttc
     4081 ctggcgatac tatccccatt attcctggtt ctgcactaaa ggcaatgcag tgtggttgtg
     4141 ggaaagcgga ttgtgaggca tgcgggccaa tacttaaatt agctgagacg cttgacactt
     4201 atgtgcctga gccaaaacgt gacaaggata aaccgttctt gatgcctatt gaggatgtat
     4261 tctctattac aggacgcggt acggttgtaa caggaagaat tgagcgtgga atggtgagaa
     4321 ctggtgatga agttgaaatt gtaggttttg gcgaaacaaa aaagtcggtt gttaccggtg
     4381 tagagatgtt cagaaagata cttgacgaag gtatggctgg tgacaatgta ggttgtttgc
     4441 tacgcggtat tggcaaagaa gaggtggaga gaggacaggt attagcaaag ccaggttcta
     4501 taactcccca tagaaaattt gaagcagaag tatatgtcct gtcaaaagaa gaaggtggac
     4561 gtcatacgcc attttttact aattaccgac ctcagttcta tttcagaaca actgatgtaa
     4621 ctggtataat tcagcttcct gaaggtgttg aaatggttat gccgggtgac aatattaaga
     4681 tgactgttga gcttattact cctgttgcta tggaagaaac acttcgtttt gcaattcgag
     4741 aaggtggtag aactgttggt gcaggtgtag ttgccaagat attagaataa gactgttcta
     4801 tttagtatga tagcaatggt agtagttgtc aggctggtat atatcaacct gacaattgtt
     4861 tagaatagta tgcaaaatga taattggtgg tgggattgta gggatacaat cagggaactg
     4921 gatgattact actatattaa tttatgagga tataagaagg atattacagt ggctaagctt
     4981 gcgggacaac gtataagagt gaaattacga tcatttgatc catatttatt agatcaatca
     5041 gcagctaaga tagttgcgac aactaacagg agtggtgcaa aggttgcagg gcctataccg
     5101 ttacctacac gtattgaaaa attttgtgtt ttgcggtctc ctcatgttaa taagaagtcg
     5161 cgtgaacagt ttgaaatccg aactcacaaa cgcttaattg atattattga tccaaattcc
     5221 gacactgttg aagcgcttat gaaattagaa ttgcctgctg gtgtttctgt agacataaaa
     5281 tcataaattc aacattgata tgatgttagt taaagagctc tttttagaag agtgtggttg
     5341 tcttataatc gatgtatggg ataggaaaat gaaaaaggcg ttaattggta gaaaaattgg
     5401 gatgagtcag ttgtttttag atgatgggac ggtaatccct gttactgtat gtgaacttgg
     5461 tccttgcgtt gttgttcaga aaaagaccat tgaaaaagat ggatacaatg ctttaaaatt
     5521 ggggtattgg gaaacaaaac ctcaccgtct tactaaacct ataatagagg atttgaagaa
     5581 gaagaatctc ccaattgtta aagtgtttaa agaagttgat atttttgatg aaaatctggt
     5641 tgaagggagc gtaataacct gtgaaatctt ttctgagaac cagatagtgg atgtaaccgg
     5701 tacatcaaaa ggtaagggtt ttgctggggt tattaaaagg cacgggtttc atggcggaaa
     5761 agaaacgcat ggttcaaatt tccatagagc acctggatca attggtgcga atacatatcc
     5821 cgctgaggtg tggaagggga aaaagcttcc aggtagacac ggtggaaaaa cggtaaccgt
     5881 taaaaacctt aaagttatac gtattatgaa agataaaaat ctggttatta tttcaggtgc
     5941 tatccctggc accgtcaatt ctattattac agtacagagt aaataggcag gattgttatg
     6001 gttgtagatg tttatacact tgaaggaaac ataaaagggc agattgagct gaatgaatca
     6061 atctttaatg caaaggttaa tgatgttttg atgtatgaat tgattaaagc agctaatgcc
     6121 aatttgcggc aggggacaca cgatacaaaa gagcgatcat atgtatcggg cggtggagca
     6181 aaaccatggc ggcaaaaggg gacgggaaga gcacgtcagg gtagtatccg tgctccacag
     6241 tggaaaggtg gtggtgcggt gtttggacct catccaaggg actatcgcat agaattgcca
     6301 aaaagaatta aacaggaggc atatcgttct cttttatcaa tgaaatttaa agagggctct
     6361 ataaagataa ttgaggattt taaagttgta gatggtaaaa caaaagaagt tgctgccatt
     6421 gctaaaaagc tcaatattag caaaggagta cttattactg atattgaaga caatttattg
     6481 aaacgtgcaa tacgaaatat accatggttt ttatataata atgttaatcg tttgtctggc
     6541 agggatattt tttatacaaa gactgtgtta attaccgaaa acgcagcaaa gattattaat
     6601 tcgaaatatg caaaaggtga ataattatga atgccaatga tattattata cgcccggtga
     6661 ttacagaaaa agcaacggaa atggcaaagc agaataagta tatattcaga gttcataaaa
     6721 aagctaataa agatatgata gggcgagcaa taaataacat atttggcgtt atgcctgtga
     6781 gaatcaatgt tatgatcgta cgaggtaagc gtaagcgtgt acgttataat tatgggtata
     6841 ctgcttcatg gaaaaaagcg atcgttacat taaaagaagg tgagaagatt gatgtttttg
     6901 aaacaaaata aggaattatc agtatagcga taaaggtaac gaatatgggt ttgaaaaaat
     6961 atagaccaat aacaccgaca ttacgatatc aaacggtttt agattacagt gagattacta
     7021 ctgatgaacc attgaagtca ttaactaaag gtaagaaagg ttatgctggt agaggcttca
     7081 aaggacagat ctccgttcgc agaaaaggag gtgggcataa acggcggttc cgactcattg
     7141 attttaaacg taataagtat aatattgaag gtaaggtgtc tactgtcgaa tatgatccta
     7201 atagaagtgc taatattgcc ttgataatct acaaagatgg ggagaagcga tatattgttg
     7261 cacctttttc tttgaaagta ggggatacag tagtttcagg ggataatgtt gaagtaaaac
     7321 ctggaaatgc tatgccctta aaaaatatac cagacggtac gtctatctat aatatagaat
     7381 tacataaagg caaaggtggg cagttggtgc gttctgctgg agtaagtgca gtcattaccg
     7441 caaaagaagg ggaatattgc ttgataaaac taccatcagg tgaaatcaga aaaatttact
     7501 gtgagtgtta tgctacaatt ggtgaagtga gcaataaaga ccatattaac attgatattg
     7561 gtaaggctgg tcgcaacaga tggatgggta aaagacctaa ggtacgtggt gttgcaatga
     7621 atcctattga tcatcctctt ggaggtggcg aaggaaaatc atcgggaggg cgacatccct
     7681 gttctcctac tggcaagcct acgaaaggat ataaaacacg aaagaagaaa aaatatagtg
     7741 ataaaatgat tattaaacga aggaagtaag ctatggctcg ttcattgaaa aaaggaccgt
     7801 atgttgacat aaagctgttt aaaaaaattg aagaaatgaa ctcaaccaat gcaaagaagg
     7861 caattaaaac atggtcacga agatctacca tatttccgga gatgattgga cataccatta
     7921 tggtacacaa tgggaaaaat tttataccgg tgtacgttac tgagaatatg gtagggcata
     7981 aattgggtga gtttgcacca acaaggacat ttagaggcca tgctggcaaa gatgacaagg
     8041 tagcgaaaag aggcaagtaa atggaaacga aagcagtatc acgatataac aggatctctg
     8101 cggtgaaagc acggttagtt gccaatgaaa taagaggttt ttcatttcct gaggctattg
     8161 atattctgag ggcaataccg cgtaaagcat caaagctttt aatgaaaaca ctctattcag
     8221 cagctgcagg tgcaaaatat cgcaatcctg agatacgtga aaataattta tacatcaaga
     8281 agattgtaat tgatgaaggt ccaacaatga agcggttcag gccaagggct cgtgggaggg
     8341 cttcgcgcat acgcaaacga ttgagtacaa ttgagatagt cttatctgat gaaaattaat
     8401 gtaagtttaa ggtgtaataa tgggacagaa agttaatcca acaggtgtaa gagtaggcat
     8461 aatcagaaca tgggattctg tttggtttag tgaaaaggat gaatatcgta agaatctcca
     8521 tgaagatatt caggttagac gatatataat gaaaaagttt aaaaatgcag ggattgcaaa
     8581 agttggtatt gagcgatttc ctgatagagt caatgtttat attcatacag caaggccagg
     8641 gttgttaatt ggaagaaaag gtgtagatat tgaaaatctg aaagttgatt tacaaaagaa
     8701 gattaacaaa aatgttaata tatatgtaca ggaagttaaa aagccagaaa aaatttctaa
     8761 attggtagca gaacaggttg cttcacaaat tgaaaataga tttccttata gaagagctat
     8821 caagcaggct attactgcag ccatgcgaag tggggcatta ggtatcaagg ttagtatatc
     8881 cggacggttg aatggggctg aaatggcccg atatgaatcg tataaagaag gaagaatacc
     8941 attgcataca ctgcgtgctg atattgatta tggatttgct gaagcattaa caacatttgg
     9001 tctgattggc attaaggtat ggatttacag tggtgatgtt ctctcaaatg ctggagagca
     9061 agaagaagat aaatttgcat tacgaaaaca atcacgataa ttgatgaagg tataatatta
     9121 tgttaatgcc taatagaacc aggtatagaa aagttcaccg tggacgtatg aaaggaaaag
     9181 caacacgtgg ttcaacaata cattttggtg aatttgctct aaaaacgatt gaatgcggtg
     9241 aaattaattc ccgacagatt gaagctgccc gtattgcaat tagtcgtaaa gtaaaacgtg
     9301 gcggtaaatt atggataagg attttccctg acagaccatt taccaaaaaa ccggctgaaa
     9361 ctcgtatggg taaaggtaag ggaaatcctg aaggttttgt tgcgcggatt aaacctggaa
     9421 gaatattgtt tgaaatttct ggtattgatg atgccatggc gcgtgaagca ttgtcattag
     9481 ctgcacataa attaccagtc aaaaccaaaa taatatcaat acatgataac aattagtatt
     9541 ggagttgatc aatgaaaggt aaactggatg aactaacggt agaagagctt aatagaagtt
     9601 tgcgtgaagc aaaagaggaa ttaaggaaag aacgttttaa gagaacagtt agcagggttg
     9661 aaaatccaaa aaaaaccatg caactgaaga agcagatagc aagaatatta acggtgctac
     9721 gtgaatatga atcaggcaca agaacagcaa gaagtaaata attttggtgg taacagctat
     9781 gcaagaaaac agcagaaaac agattgttgg taaagttata agcaataaaa tgaacaagac
     9841 ggtagttgtt gaaattgaga gaatgatgat gcatcctaaa tatcataagt atgtgcgaag
     9901 aaccaaaaag gtcaaaagcc atgatgaaaa gaatgaatgt tcaattggtg atatagtact
     9961 tatagaagaa accaggccgt tatctaaaga gaaaagatat cgtttggtga agatagttga
    10021 aaaggtaaaa tagggttagt gctatgattc aggtacaaac aatgttagaa gttgcagata
    10081 atagcggtgt gaaacgagtt cagtgtataa aaattttagg gggaactcga aggcgttatg
    10141 cttcggttgg agatgtcatt attgttgccg ttaaagatgc acatccatca tatggattga
    10201 aggattcgac agggaaaaaa gtccatacaa aagctgtttt gagggcagtt gtagtaagga
    10261 caaccaagcc ggtacggcgt tctgatggtt catatatacg gtttgatgat aacgccgttg
    10321 caattattga taataaaggc gagcctcgag gttctcgtat ttttggtcct gtagctcgag
    10381 agttgcggga tagaaatttt atgaagatta tttcattggc accagaggtt ttataaagga
    10441 ttaagcttat gataagaaca atattaaaaa agaatgatat ggtaatggtt attaccggtg
    10501 cagataaagg taaacgcggt aaaatacttt ttgtagacag aaagcgagga agagtggtag
    10561 ttgaaggtgt taatgtaagg aagaaatttg tacgtccttc acaggataat cctaaagggg
    10621 ggcagataga attgccatat ccaattaata tctctaatgt tatggttttt tgtgataaat
    10681 gtaaaaaagc tgttcaattg ggtatgaaga tagatggtga caccaaatat cgaatatgta
    10741 aaaaatgtgg caaacgaatt gattagtgag ggtaatgatg gcagcacgat tgcgcatggc
    10801 atacgaaaag gatgtaaaaa aaagaatgat ggataggttt aaatattcat ctgttatgca
    10861 ggtcccaaaa attgagaaga tagtattaaa tgttggtgaa ggtgaagctc attcaaatcc
    10921 taatcttttg cagtctatta ttaatgaact atcacttata accgggcaaa aggctgttaa
    10981 aacagttgca cgaaaatcaa ttgctgcttt caagattcgt gaaggttatg atgttggtgc
    11041 tcgtgttaca ttgcgaggcg atagaatgta tgaattttta gatcgactta tcaatgtagc
    11101 cttaccgcga gtcagagact tcagaggact atcgccaaac tcttttgata atgcggggaa
    11161 ttataatttt tctgttaaag aacagataat attcccagaa ttaaatctgg acaaagttga
    11221 gaagatttat gggattaata ttacaattaa aaccactgcc agaaatgcta atgaagccag
    11281 ggcattactg gaagaattca gatttccatt ccaagaagcg aggtaagttg aggtggctag
    11341 cgaaagaatg tttgcaaagg ctagaagaga gcctaagttt aaaattcgtt acaggaatcg
    11401 atgcaaaatt tgtggtaggt ctcgtgggta ctatcgccgt tttgaacttt gcaggatttg
    11461 cttgcggaag ttagccggtg aaggtttggt gccgggcgtt aagaaatcgt catggtaaaa
    11521 tttaaaagag gtaaataaga tatgagttgt gtttctgatc caattgcaga tatgttgacg
    11581 agaatgcgga atgcaattaa tgcaggacat gtgaaggttg atataccatc atctaaattg
    11641 aaaatggcta tcgcaaagat tttaaaagat gaaggattta ttaaaaattt taagttaatt
    11701 gatgataata agcaaaaact tttgcgtatt tatctgaagt atagtgcaga taatcagtcg
    11761 gcgattattc ggcttaaacg gataagctcg cctggaagaa gggtatatct taaagctgag
    11821 aatttacatc cggtttataa caatatgggc attataatat tatctacatc aaaaggtatt
    11881 attaccaata aagccgccaa acaacttaat attggtggtg aagttttatg cgagatatta
    11941 taaggggcag caggatatgt caagaatagg gaaaaaaccg atagtgatac cgaatggtgt
    12001 atccattgat atgcagaata ataatttaac cgttaaagga cctctggggg tacttcaata
    12061 tcagttgcag gatgtgataa atattgcaat tgaaaataac actattgctg tttcaaaaaa
    12121 agatgaaaaa tctaatgaca aaaaagttaa tgcattgtgg ggcttaacca gggcattaat
    12181 taataatatg gtaattggag tatcaagtgg ttttgaaaaa aatttagaga tagttggtgt
    12241 tggatatagg gcaatacaaa aaaataatga tgttgaattt caattaggat attcacatac
    12301 aattttgttt actcctccgc caggtacaaa ggtagaggtg gtagaaccaa caaagataag
    12361 agttaagggt attgataaac aacttgtagg tcaggtatct gcaaatattc gaaagctccg
    12421 cccgccagaa ccttataaag gaaaaggcat tagatataag gatgaacaga tactgagaaa
    12481 agctggtaaa actggtaaat aatgctatga gaatgagaat atgaaggata gttattatga
    12541 atagattgaa attaaaaaaa gtcagatata tgcgcagaag gatgcgtgtt aaagcaaaag
    12601 tcagaagcaa tatgcagcgt cctcgcatgt gtatttcccg aagcaataag aatttatttg
    12661 tgcagattat cgatgatggt caaggaagta ctcttgtttg cgcttctacc cttgaaaaag
    12721 attttccggt tgtaaaaaat agagggaata aagaggcagc gaaagcactg ggtacaataa
    12781 ttgctcagcg agctattgca aaaaacataa aagaggttgt atttgataga aatggttatc
    12841 tttatcatgg taggataaag gagtttgctg atgcagctcg tgaaaatgga ttagaatttt
    12901 aggggtgata tggatgaaga tcacaagaaa acgtataaaa ccggaaggat tagaacttgc
    12961 tgaaaaaata ataataatca accgagttgc aaaggttgta aaaggcggta gacggttttc
    13021 atttaatgct atgtcagttg ttggtgataa ccatgggcat gtgggcattg gttttgggaa
    13081 agctaatgag gtgcctgatg caatccgtaa aagtgttgag gatgctaaga agaatgttta
    13141 taaaattaat gtgaataaaa ataccattcc tcacgaggta ataggcaggt ttaaatctac
    13201 aagggttgtt ttaaaaccgg ctgcacctgg tactggcata atagcgggtg attcggttaa
    13261 ggcagtagtg gagaaggcgg gaattgttga tattatgtca aaaacattgg gttcacgtaa
    13321 tccggttaat gttacaaaag caacaataaa tgggttgtta caattacgta gcttaaaaga
    13381 agtatctgat ttgcgtgaaa ttattttacc aaagctgtgg caatcataag aggtggatat
    13441 atggctaaga aattattgat aaagcaaaca cgtagttata taagcgcaaa accaaagcaa
    13501 aaagctacat tacgtgctct tggcttgcgt aagttaaatg ctgaaagagt acatgataat
    13561 actgcagtta tacgtggtat ggttgataaa gtaaaacata tggtaaaagt ttcagaaata
    13621 aatgaataac tatgggtata aatgatatgc aatcaacaat aaaaaaacct gaatatatta
    13681 aaaaaagtaa acgagtagga tgcggtcagg gtagtggtca tggtaagaca agttgcaaag
    13741 ggcataaagg gcagaaagca cgttcgggag cacattttaa acctggcttt gaaggtggac
    13801 agatgccatt gcaaagaaga attccaaaac gtggatttaa taatacaaaa tttaaagaag
    13861 aagttcaggt agtaaatatt aatgatataa caaaattgtc tgtaaaagaa ataacccctg
    13921 aagtacttta taaggaaggt ttaattaaat cacaattttt accggtaaag cttttagcag
    13981 tgggtgacat caatgatgcc attgctgttg ttgtagatgc tatatcaaca actgcaaaag
    14041 ataaaattat aaaagcaggt ggtagtgtaa cactgcgagc ataatatggg acaagaggtc
    14101 ataacactat aatgccaaat gtaatttcaa atatatttaa aatacccgag ttacgaaaaa
    14161 gagtattgtt tacccttttt attctggttg tttacagaat aggcgctcat atccctattc
    14221 ctggagttaa tattgaggct ttaagttcat attttgaaat ggctgctaaa agtcgtggta
    14281 gtggttttgt agattttttt gatttatttg caggtggtgc attaaaacgg tttaccatat
    14341 ttgcattagg aattatgcct tatatttcag catcgattat tatgcagctt ttaacagttg
    14401 ttatacctgc tattgaaaag ttatcgaagg aaggcccaga aggttataaa aagataaatc
    14461 agtatacccg ttatggcacg gttctcttat gtgcattgca gggttttggt ttgactatgt
    14521 ggatgaagtc aatgacttca ccaaaaggtg cagtagtccc acaggattct ggtataggat
    14581 ttgtactggt agctgttttg gcaattacta ccgggacaat gatattgatg tggttaggtg
    14641 agatgataac tgaacgaggt atagggaatg gaatatctct tattatatat gcagggattg
    14701 ttgtacgtgc tccttcagcc tttgttgata ctatgaatag gattattagc aggagcgatc
    14761 caattattgc cttgatttta attgccctgt ttattggggt tattgctgcg tcaattttat
    14821 taacattagg tgtaagaaaa attcccgtac aatatgggaa gagggttgtt ggcagaagaa
    14881 tggttcagat ggcatcacag gcaataccct tacgagttaa tgctgcaggc gttatcccta
    14941 ttatttttgc atcatcaatt attttgttcc ctgcacagat cagcaatatg ttaggagcta
    15001 caaaatatgg catattcaca aagatgcaat actggctgtc accagggcat acagcgtata
    15061 tgattatcta tagtcttttg ataatatttt tctgttattt ttatacagct atacagttta
    15121 atcccaatga tttagctgaa aatctaaaaa agtatggcgg ctttatccca ggaataagac
    15181 cgggacaaaa tacagctgat tatataatgc atattttaaa tagaatcacg ttgccgggtt
    15241 caatattttt agcattgatt gcaatatttc ctgatttcat tatacgaata tggccagaag
    15301 aagtatctcc ggaaatggca tatctctttg gtggtacttc attgcttatt attgttggtg
    15361 ttgctcttga tacattaaaa cagatcgagt cacaattgct tatgcgtcat tatgatggat
    15421 tctttaaaaa gggtcgtata aaaggtagaa gatattaaat ttttagctat actatgtttt
    15481 tagtatgttt ctatattgag gtattttggc tatatatatt tacaatgagg acgagattaa
    15541 acgggtagaa gatgcgtgca gaataacagc agcggttctg gaagagttag gtaagaatgt
    15601 tgttgagggt attagcactt ttgagttaga tagagtagca cgtgatttaa taagaaaaag
    15661 caatgcaatt ccagcatttt taggatataa ggggtatcca gctgcaattt gtacatcagt
    15721 gaatgaagtt gtagtacatg ggatacctca tagaaatcag attttaagaa aaggtgatat
    15781 cgttggtata gatattggtg ttaagtttaa agattttttt ggtgatgccg ctaagacgtt
    15841 tatcgttggg aaggtaaaca acaaagtaaa aaaattactt gaagttacca aagaggcgct
    15901 ggaattaggt attttacaat gtaaagacgg gaatcacctt actgatatat cccatgtaat
    15961 tgaatcccat gttgtgcagt ggggatttca gcctgttcat gagtttgtgg gacatggtat
    16021 aggtagaaat atgcatgagg aaccttctat accaaatttt ggtaaaccgg ggaagggacc
    16081 tataataaaa acaggtatga tctttgcaat agaaccaatg attaatgaag gtacgtatca
    16141 ggttgaaatt ttggatgatg gatggacaac cataacaaaa gacaggaaaa attcagctca
    16201 ttttgagcat acggttgctg ttgtacatgg aagtccaaaa attttaacaa tggtttagaa
    16261 aacaaaagag gagtataaag ccatatggcc aaagaagaac ctattgaagt tgaaggagaa
    16321 gttgttgaag cgcttcccaa tgctatgttt agagttaagc ttgataatgg tcatgtggta
    16381 ctggctcata tttcagggaa gatgcgaatg cattatatca gaattttacc aggcgataag
    16441 gtaactgttg agctttcgcc atatgatttg agtagaggca gaataacgta tagagcaaaa
    16501 tgatatatga gagcttgagg taaactatga aagttagagt ttctgttaaa aaaatatgca
    16561 gtgattgtaa ggttataaaa cgacatgggg ttgttcgtgt gatttgtaaa aatccacggc
    16621 ataaacagcg gcaaaagaaa agaacagcga aataatacaa tatagaggtt tatatggcac
    16681 gaattgcagg tgttgattta ccaaataata aacgtgtaga aatagcactt acctatattt
    16741 atggtatagg gcttccctct tctctgaaga tcttggaaga agcaagggtt aatcctgaca
    16801 caagggttaa ggatcttacc gatgaagaga ttaaccagtt acgaaaaatt attgaaaaaa
    16861 gttataaggt tgaaggcgat ctgcgtacac aaattgcaat ggatataaag cgtcttatgg
    16921 atattggttg ttataggggt atacggcata gaagaggatt gcctgtccgt gggcaaaata
    16981 ctaaaaataa tgcacatact agaaaaggca atagaaaagt ttctgttggt ggcaaaaaga
    17041 aaaaatagta actgaataac agaggtatac agtgaagcag aaaagaacga aaaagcagga
    17101 aaaaaagaat ataccggtag gaaaagcgta tattcaggct acgtataata atactattat
    17161 aacaatcaca gatatgcagg gtaatgttgt atcgtgggca acatctggtg gcgagggttt
    17221 tagaggatca agaaagtcaa caccttttgc tgctcaaatg gctgctaaag ctgcagcaca
    17281 aaaagcaatt gataatgcta atatgcgaag tatagaagta tatgtaaagg gtcctggtat
    17341 agggcgggag gctgctatcc gctcattata ccaggcaggt ttaaatatat caaagataaa
    17401 agatattaca cccataccac ataatgggtg tagaccacgc aaacgaagaa gagtataacg
    17461 tgaggtttta atctatggca aaatatactg ggcctttgtg taaattatgt agacgggaaa
    17521 tgactccatt gatgctgaaa gggagaaggt gcttaaccga taagtgtgct attaagcata
    17581 aaaagtatcc tcctggacca cctaaaaagc gtcgaattaa attatcggat tacggagtgc
    17641 agcttcgtga aaagcagaaa attaaacgaa gttatatgct tttagaaaca cagtttaaac
    17701 gtgtttttga agaggctacc cggttaaaag gagttacagg ggaaaatatg cttgtattgc
    17761 ttgaaagaag gcttgacaat gttgtataca gaatgggatt tgcatcttct cgtttgcagt
    17821 ctcgacagtt ggttcaacat gggcatgtaa taataaatgg caaacgtgtt gatataccat
    17881 catatcaggt aaaggtaaat gatattgttg agattaaaga agattttaaa aataatgcta
    17941 ttttagaaga tgcaataaaa ttgtcaaaag ctgttggatc ggttcctcag tgggtagaag
    18001 ttgattatga aaagaggaat gggaaaatta tcaggttgcc tgagcgaaaa gatattgagc
    18061 ctatatttaa tgagcaagct gttgttgagt tatattctaa gtaagacttt agaatagtta
    18121 tgtgtttatt tactggaggt gataatgtct tataagaacc tattaaaagg ttttcgaaga
    18181 ccatcgaaaa ttgtttttga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 cagttgatga aagccatgat aaattaaaag ctattcttgc taaatctata gatgaacttg
    18361 aattctctgt tcgtgcatac aatacattga aaagtttaga actcaacaca ttagaagatg
    18421 tggtgcataa aacagaagat gaattaaaaa aatcaaaaca tttcagtgag caggttatgg
    18481 cagaaattaa gaaaaaatta gaagagtacc atttgagctt aggaataaag gaataagagg
    18541 tggcatcatt atgagacatc gtaacaacgt acgaattttg ggacgatccc atgaacatag
    18601 aaaggcaatg tatgccaata tggtaacgtc gctattccaa catgaaagga ttattacaac
    18661 aaagcaaaag ggtaaagaac tgaagcgatt agccgaacgt ttaataacgc gtgcaaaaga
    18721 aaacttgaat cttacagaaa atgataatgc taaaaagctt cataataagc gtgaggttat
    18781 gcgcttgatt cataatgaag atgttgtgaa gaaacttttt gatgatattg caccacgttt
    18841 tgtaacaaga ccaggtggtt atacccgtat gtatttgctg gatagaagac agggtgatgc
    18901 tgctgaaatg gcaattgtgg aattagtagt taaaactgaa aaaaagcagg aagaaaaaaa
    18961 agataaagag aaaaaaaata aagagaaaaa atctaataaa acaacatagt agtaaattgt
    19021 tgaggcaggt ttaacccctg cctctttata ttattttgtt gttttctttt ccaaacggga
    19081 tttttcgatt gtatgcacta aattaagatt atcattagca tctactttga attcttcagg
    19141 aatacgatca tcaaaaatcg gatcgagttt attactttcc aatccatact tcgattgtat
    19201 agcatctaac tgatttattt ttatcatttt atggatttca ataccatatt gttttgccaa
    19261 tctgcaaaaa tcaatagccg ctaaatattg tttaatgata aactctaact catcgtgttt
    19321 ttttattgat ttagttttaa gatatataat atcctgattg ttataaatgt ttttagcttc
    19381 ctgtaatttc cggtaaccat ttttcaagta gcgctcaatg gtttctctat caaaaaaaag
    19441 atgatattct tctggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngataa
    19561 tgttaaaaaa taatcgtttt gtgctttaac agccgttgaa attgcaaata gtggctgtgc
    19621 aacgcacacc ataatcagta caataataag cttgatagta tggttcttca taaacattac
    19681 cttcggtgat attcttttta aatttatcga cacgcgaaag ttttcgcttg atataaaaat
    19741 atcataatgc tttatctaaa atagaaagtc tattagattt ttatgaaatt tcacaggaac
    19801 tatcgccaaa aatttatttt ttgatagtta ttgggcaatt atttagttgc aaagattatc
    19861 tggtatgata gttaatgata aaaatgatag tagtacaaat atcaggatgt agggtatgga
    19921 aaaagaggat agtaaaaaga agatatcatt ccgacagaaa aatgctacaa tttgtccggt
    19981 atgtggatat gagttttatc gtgaagaaat gctaacagga ggtgggcggc ttattgcagg
    20041 gaagctaacc gatgaattgc ggcgcaccta tgaaaagaat gaaaaatggg gtgttattta
    20101 tccactggca tatgtagtaa ctgtttgtcc tcgatgttta tatgctgcct atcctaaaga
    20161 tttttctaat ttacagtcag aagatttgca gaaaattcag gcaacagcga atgcacgaaa
    20221 acaatcaata gaaaaatttt ttgggaagct ggattttaat atcgataggg gtttatatca
    20281 tggagctgct tcatatcttc ttgctatgga ttgctattca tttagaaata aaatggttgc
    20341 tcctacattt aaaatggcta tatcagcaat acgtgctgca tggctttttg gggatttagc
    20401 taaactggag cctgatacgc catataaaaa gatttcagat ttcttttata aaaaagcgta
    20461 tgacctttac tttaaagtgg tggatattat gcaaacagga gccgagccgg tagatgcagc
    20521 ggggaatatt gggcctgata ttgataagaa ctggggatat gatggtattt tatatatgtg
    20581 tgcaatactt acattaaaag ttggtgctaa agaacctgat ttgaaaaaaa ggattgaaaa
    20641 ctttgaaagg acaaaacgtt acctgagcaa actctttggt agtggaaaag catcaaagaa
    20701 taagccgggg ccccttcttg atatgacccg cgatttgtat gataagatga atgagatgct
    20761 ggaacaatgg aagaacgaga ctcccgtctg aggtatgcgt taattgtcca atatgatggt
    20821 acacactata gcggatttca aatacaacat aatgctaata ccatacaata tgaactggaa
    20881 aaagcaatca agatactaac cagggaggat ataaggattg ctgcagcagg cagaaccgat
    20941 gctggtgttc atgcgttagg gcaggttatc cattttgatt tgtccaaact catcccctta
    21001 gaaaagatgt gtattgggct taatgggatt ttacctgctg atatagctat aaaaaaagca
    21061 tttaatgttg atagccattt tcatgcgcgc tatagtgctg ttgctcgtga atatagctat
    21121 gtactttata atcatagata taaaagtccg tttatgcact atagagcgtt atgggtacgt
    21181 gaacctatca atattgcatt ttttaaagaa gcctgtcagt attgcattgg tgaaaaagat
    21241 tttgcatcat tttgtaaaaa gtgttcagcc acagagggta ctatacgatt tgtggaatac
    21301 attacagttg atagaatctt taatgatttc gttgttttaa caataaaggg aaatgctttt
    21361 ttacacaata tgattagaat acttgtcggg acactcctta tgctgatgaa taataatgag
    21421 catcctctta agatgaaaga gatattagac agatgtgaca ggaattatag tggtgaaaca
    21481 gctcctgcat atggattgta ttttaaaaag gtatattatc cggcagtata tgatttttta
    21541 aatgtgtaac taaactgtat gttatgatac agattataca aaaggttaca ggcaataaag
    21601 ttaataccga taactatcgc cgaaagtatt ttaaaaatag tgatgagcat taaatatgta
    21661 ttgacaggta taccaagtat gcaaagttta gtggtaatta ataaaatagg ggtttctggt
    21721 gaaaaataat gtattgtatg gaatgctaac tatactgctt gtttttatta tagcaggcaa
    21781 tggatatgca cagcggaaaa agggagatta ttttttaaaa ccgcagattg gcatgtggtt
    21841 tggtccggtt acaccagttt tttcaaccgc cgatgatgtt gatactgatt tatggggtgg
    21901 cggttttatg cgcttcaaca tatcgtcact tcctttaaaa attggatttg attcttcgta
    21961 tgaacatttt aaatc
//
LOCUS       DAOOJH010000033        21672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2445_length_21672_cov_9.711338, whole genome shotgun sequence.
ACCESSION   DAOOJH010000033 DAOOJH010000000
VERSION     DAOOJH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_21672_cov_9.711338"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /gene="metG"
                     /locus_tag="PLJ75_06210"
     CDS             <1..1782
                     /gene="metG"
                     /locus_tag="PLJ75_06210"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPD04847.1"
                     /translation="GTDEHGDKIVKAALHNNTRPQEYTDKISAIFKEQWKSMGIEFND
                     FIRTTEERHKKVVQYVLQKVYDNGDIYFGSYGGYYCFGCERYFTEKEMVDGKCPDHQT
                     KLEYIHEENYFFKMNKYQQWLIDYIHEHPDFIRPERYKNEVLAMLEGEALEDLCISRP
                     KARLSWGIPLPFDTNFVTYVWFDALINYISAIGYPDSDLFKKFWPVSNHLIAKDIVKP
                     HGIFWPTMLKAAGIEPYQHLNVHGYWNMESTKMSKSLGNVVTPSQLLEKYGNDQIRYF
                     FLREMTFGLDAKFSEEAITNRINYDLANDLGNLINRTFNMVQKYCDAKVPPFHSDEPA
                     NRDDMQQALQVSMHQYIDFVHQFQFNIALERLWDFVRYCNKYIDESKPWQVAKENNVK
                     KLQSILRNLLEGIHAVAILLSPVCITKSSLVIKALKSSAQCTIDTLPAMSSLSDGTTL
                     DSPGILFPRLEKETEAVNSAHSTKKEAFMETTPEGLIDIKDFLKVELRIAKVLQAKAV
                     EGSSKLIELSIDTGKDIRTIIAGLAPHYTPDYLTGKKIIVVANLKPATLLKKTSNGML
                     LAAKLSKEDAPILIEIDDKVPVGAVLS"
     gene            1809..2300
                     /locus_tag="PLJ75_06215"
     CDS             1809..2300
                     /locus_tag="PLJ75_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HPD04848.1"
                     /translation="MKIGLISDTHNDIELVQKAIALFKERDISMIVHAGDLTSARIIS
                     LFKGFQGRFVMGNCDIDVEEINKTARDCGFGCVDYACEFTVEGKHFMVFHGNNVPMFR
                     EAVNSGKYDYIIKGHTHIFENYLSGKTRIINPGALYRGEELTVAILDTATDKVEKIRI
                     DIE"
     gene            complement(2301..3170)
                     /locus_tag="PLJ75_06220"
     CDS             complement(2301..3170)
                     /locus_tag="PLJ75_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPD04849.1"
                     /translation="MAKKVLGKGLGAIITTSPTPASDFEKVLLQDKDRIVDIPVASIM
                     PNPEQPRVHFDQAEIAGLAQSIQHVGLLQPIIVRKQGDEYFIVAGERRWRACKLLGKP
                     TIRAIVMNTSEEQNLTLALIENIQRTNLDPIEEAKAYKILSTRFKLKQSEISDRVGKD
                     RATVANILRLLNLPEHMQEALQDGKINVGHAKLLLSVSMEEQEALYEKIINQGLSVRD
                     VERAIADVSKSSIKKKKKAKDAHLRKLEEELISFLGTRVAIHHSAGKGRIEISFYSLE
                     DFDRIVSIIKGER"
     gene            complement(3280..4041)
                     /locus_tag="PLJ75_06225"
     CDS             complement(3280..4041)
                     /locus_tag="PLJ75_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD04850.1"
                     /translation="MGKIISISNQKGGVGKTTTAVNLAAFLAEKGKRVLIVDIDPQAN
                     AGFGLGVNVEEMETTVYEVLIGQLPVEDAIYKTTIDNLHIVPSNMNLAGAQVELMDMD
                     DKEYLLKRALQKVRDTFDFIFIDCPPSLGVLTLNSLVASDSVIIPLQCEYYALEGLSQ
                     LLKIIAMVQDTLNPELEIEGVLLTMYDPRTNLSQQVVADVKQFFHDKVFSAIIPRNVR
                     LSEAPSFGKPINLYDRSSSGSVSYEQLAMELLRSR"
     gene            complement(4313..5503)
                     /locus_tag="PLJ75_06230"
     CDS             complement(4313..5503)
                     /locus_tag="PLJ75_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04851.1"
                     /translation="MRIALLLLMLLLSLPLSAAPRVLVLKSYHDNLTVHLDKYHIPYI
                     VKRGWAVANDSLSGFDAVFIPCGVEQPPETSIQVMAHGQRIKGVSLKDDYAGIDYKRL
                     NALLQDFVNNGGRLYAAGYSYQFVEAMGCSLSFFNNFPHFGTGGQIHCFLNDDFASFV
                     MRNDIQLPVSYNGWVALENADAVILAKGYFPTVQGEREGPLAFYKQYGKGRVYYSVYH
                     SEIDSLYMRYFIFRVAYGALLDEMFHHAWYWGQKVVFSVVDTFTSNEYARSYRCTLPR
                     GTATIYLPSDCSLQVDIVAEKTIIGSWDSGQQKQITFYNATPREVIVSVYQAARIPYQ
                     PFSLVIAKGKRMVPYNPVVKVSAGALAIVMVIGLTWKRLNPRRFTGKRRYWFRDGHIL
                     NKQK"
     gene            complement(5500..7362)
                     /locus_tag="PLJ75_06235"
     CDS             complement(5500..7362)
                     /locus_tag="PLJ75_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04852.1"
                     /translation="MENDLKALKRKAKALLLSKDIPAGKMDIVRALLANDRVLEEEKY
                     RAIIDLVKNYPDKPVMPVSPGKVEVKGADEFKKTIQYDSKNTNVLYHKYKHTRLFKKR
                     LLVHANNKFGIGFLRRLIPSQKFLKVSQRLFDLQSALRQRLYKILTDIVDDQSIDNPV
                     IFNYCVVLYNALESVCLLNKHYMELKWTGPEEYKQLLSPYIESLLKLSLLDTGTKEAI
                     IQLIDQKMRESEDLQKKPENINKKNLSIEQYIYDFVTTIRCFLYPAITNDCSLIAILK
                     KETSLPSFESFCVACVEALITGKPVALSDIINMFYVSAPKVSDTAWNFDHDELVKAGK
                     DAESLKRKAKEVLHKKLNPYDELFRFLTYAPEGKEVMIQVFDDQRKIVDKRNELYEDT
                     YSKDIVMLLEGMLLGFINEYGSMLDGSMLYFENGNNQYEGCIFTESYFMHEFARFAEI
                     LSEINIFRTNNPTFHVTRDELIEGINKKKKSLYPIERFMTVIGDMFYDMGIELFMLYN
                     RHRNWILNGAPGSNDVIMRTAFSARTSGVLPFDDDEATPIPFSDCLISGFTKSRAMSK
                     LLLHKPVIHATLRDGVFPQMTAFCLQVAHLCLNDRLYGHLDERTRILTQIRGLA"
     gene            complement(7690..8313)
                     /locus_tag="PLJ75_06240"
     CDS             complement(7690..8313)
                     /locus_tag="PLJ75_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04853.1"
                     /translation="MMKKITTILIVAFFSIYFMGNSLYSYQKKYIFKDQTIIDEKTGI
                     TIMTNETKDLIIVTNYILQYAIIFPYNNQWEFDINDKYAIIGNCGTINISLQIHENNN
                     KTTEEFLSDLMKIYTENKSKCGVIDAKLIQYNKDLVLRTVVDPTIIYKDDTFKNVKHI
                     NYYSTKTFNNIRYVYHSSVIVDNDKKINEEDFLNFATRGFFIDFGRK"
     gene            8711..8929
                     /locus_tag="PLJ75_06245"
     CDS             8711..8929
                     /locus_tag="PLJ75_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04854.1"
                     /translation="MLHIPIGQKLSLDEIVSLNVTIAIMYNRLSKRTLMRDVKELIDL
                     ELLLFDNNTYYANIDLLKKNISHRKKKR"
     gene            9067..9450
                     /locus_tag="PLJ75_06250"
     CDS             9067..9450
                     /locus_tag="PLJ75_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04855.1"
                     /translation="MKTVKKQIIAIFIATVWISVSEFARNEFIVKSYWIQHYNKLGLV
                     FPSQPINGAVWGIWSLCFAIAIFFIAKKFSLIQTTFLSWFAGFILMWLVSWNLNVFPI
                     AILPFAIPLSLLEAFLASFIVKKLT"
     gene            complement(9557..10780)
                     /locus_tag="PLJ75_06255"
     CDS             complement(9557..10780)
                     /locus_tag="PLJ75_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPD04856.1"
                     /translation="MRIYTMKLISFFTWLFIFTGYCFSQELSPITIVATGNNASGFAL
                     FKQIDGKQKDKNIFISPFSIAMAFGMAYEGASGNTAREIASVFAFIPDDKDRWNAFST
                     IYKTLNPDNAKYKLAIANAFWVQQDYPLLKTYTDILKNYYHSEAYNVDFTHDDKRKEA
                     VKKINAWAANNTYDKIREAITLDDVKPLTRLILTNAVYFKANWLYQFDKNQTKVDNFY
                     IDSNKKIKTPMMMQRNVTIKYMENNNIQMIELPYEGKALSMFVILPKKDIKSIMPLLN
                     DKNFESWRQNLIQEEVDVYLPKFKVECGYMLVEPLIKMGIKDAFNEMKADFSKITGGK
                     DLYISKVIHKTFCDVNEEGTEAAAVTAIVMNTKSMVSFEKKIFKADRPFIYAIVDNNT
                     GVILFLGKMIKPEYK"
     gene            complement(11038..11298)
                     /locus_tag="PLJ75_06260"
     CDS             complement(11038..11298)
                     /locus_tag="PLJ75_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04857.1"
                     /translation="MKKIMIFIMVILLSGVCFNVSHCIAESALDQAKDAAESSQKAKE
                     DADKGKYEEAREKAGGQFDDEYDREKDQEYPIPEPEISNEDD"
     gene            complement(11957..13081)
                     /locus_tag="PLJ75_06265"
     CDS             complement(11957..13081)
                     /locus_tag="PLJ75_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04858.1"
                     /translation="MKKLIVVLVIAGVVIAQPLFAATITGTAPQLRVYVGGSWNTIDV
                     STEFNQAILDANAELAKYDDQDKLATGFANANMYSTNAATQQGYPNYSLFAITTGVMV
                     GAQAPTTNVDYYTNGKIEDDIENKGDLYAGVAGSAAVNVGINAGMIIPGLYINVKFGK
                     FDSTWVYDDDDFSCDTMIIGGGITYSLFDEAGTGLLRWRGLTIGTGLVYQTTDVSYRV
                     DLDSKTDSFTVDPGTGTDVTGSVVVDPSFDITLDMYTLSVPFEINTALQFLWLFNINL
                     GVGIDLVSGNADLELQSVSDATVKNIAYGGTPLPENEYIVTPGKVTIDGKTLDKKPSW
                     ARARIMTGIGLNFGAVKIDVPIIYYLKSGAAVGVTAGIIW"
     gene            complement(13414..14556)
                     /locus_tag="PLJ75_06270"
     CDS             complement(13414..14556)
                     /locus_tag="PLJ75_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04859.1"
                     /translation="MILERKYDIEEIQKRIRYYPVTAIIGPRQSGKTTLAGMLRPDMI
                     FDLENPVDMAALENPVLQLDKEKGLIIIDEIQLKPELFPVLRYLCDKNPECRFCILGS
                     ASPDIIKQSSESLAGRISFYVLMGFRKEDLPHSLLEHFHFRGGLPRSLLAPDDTTSVM
                     WRKDYVKTFLERDIPLLGIRIPPYTLLRFWQMLAHYHANVVNFSEIGRSLEISDTQVR
                     RYIEILAGTFMVRLLQPWYANIGKRLVKRPKLYIRDSGILHSLLMIESKDALLRHPKL
                     GASWEGFALEAVVRSIKKNDDEIFFYGVHSGAELDMVWQHGGYAWGCEFKFSDAPVLT
                     KSMHNAVQDLQLKKAWIVYPGQKKYKVKNTIEVIPFDDIPDVWVYD"
     gene            complement(14698..14985)
                     /locus_tag="PLJ75_06275"
     CDS             complement(14698..14985)
                     /locus_tag="PLJ75_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04860.1"
                     /translation="MIDITVIIILVVIATVLLWTVVLFKIVMSPFAIRDKWKLLGVLL
                     IFALCYSILPSWATKTLHLKSYTIPCIIVSILFVGLVILYTMINARLKKYK"
     gene            complement(14997..15173)
                     /locus_tag="PLJ75_06280"
     CDS             complement(14997..15173)
                     /locus_tag="PLJ75_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPD04861.1"
                     /translation="MNKMVKFRDVVVHQYSNVNAEIVIAILKKHREDFMKYRDAIVMY
                     LKNHGFNEYHQNNA"
     gene            complement(15187..15450)
                     /locus_tag="PLJ75_06285"
     CDS             complement(15187..15450)
                     /locus_tag="PLJ75_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HPD04862.1"
                     /translation="MVDIPLILKKLSKLEEYYKQIEEFKGVTLNEYSNDWKIQRIIER
                     TLQIMIETCVDIANHIIADNKLRIPESYADTFKVLHENTISIR"
     gene            complement(15425..15862)
                     /locus_tag="PLJ75_06290"
     CDS             complement(15425..15862)
                     /locus_tag="PLJ75_06290"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD04863.1"
                     /translation="MMHYEPIPDTVMSYIPDITKILQDEAIIFAYLFGGLARGFYTPL
                     SDIDIAIYIDDVPDVADYTFNLFCKLAEAVKTSEIDIVILNTAPESITGRIVLNKVIL
                     TDKEPLKRYIYESLTLRKYFDFKMKEEQILFRRYNIGRYTINS"
     gene            complement(15957..17213)
                     /locus_tag="PLJ75_06295"
     CDS             complement(15957..17213)
                     /locus_tag="PLJ75_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HPD04864.1"
                     /translation="MYALVDCNNFYASCEKVCNPALWGKAVVVLSNNDGNVIARSPEA
                     KALGIAMGAPFYQCTDIIRKHHVAVFSSNYALYGDMSRRVMDTLATCAPYMEVYSIDE
                     AFLLCNVPPCHAEGYALQVKHTVLQWTGMPVSVGMGATRTLAKLANHIAKKRTQSGVF
                     VFVGDSSLYKNLLVHIDVQEVWGIGGRYARMLRRHGIDTVWQLICADDSFIKKTMTIV
                     GLRTVYELRGIPCISTEDAPPPKKCIVSSRSFGKPVTECNELQEAAAHYASIASQKLR
                     KQHGAAGVVTVFVSTNPFKDEPQYSSAATITLDMHSCYTPDIIAAAQTALHRLFRSGY
                     RYKKVGIMLTDIVDAQELSPTLFSPAQSCRKRYNLMQTLDSVNAARGRGTVYFASEGV
                     QRQWAMRRRYLSPRYTTHWDEIPLVR"
     gene            complement(17216..17638)
                     /gene="umuD"
                     /locus_tag="PLJ75_06300"
     CDS             complement(17216..17638)
                     /gene="umuD"
                     /locus_tag="PLJ75_06300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HPD04865.1"
                     /translation="MCPLPVINCSSRSPIAPLYATAIKAGFPSPADDYIETVLDLNEY
                     LIRHPAATFFVRVSGDSMTPGICHGDILVVDRALTPVHNNIVIAAVNGELTVKRLVIK
                     NGAALLVPDNDTYEAMALTPEMDCTIWGVVIHIIRTVG"
     gene            17925..20147
                     /locus_tag="PLJ75_06305"
     CDS             17925..20147
                     /locus_tag="PLJ75_06305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04866.1"
                     /translation="MNSPDRYFLQPYQNNDIVLRLKAWAALIFNFSVIFAVVIMGTFF
                     AVRGIKDPGILYPFTGTIFSAIISLALLRKGKFQFAANLSFASVQIALWLVIFFESGS
                     LLQRMDSIVLISASLAYTPLLFVDRRRGIWFYYTANMLLLIIFVLYVQAALGLERWEV
                     IEYFMDSALALTFVTAISYQIFSIHKLGIINAYKAQNKIQKQYEELSATAEELEAMNE
                     ELTAINEELENTQQELLISNSNLQKETAQLQTILSSITDGIITTDDKGTITSINYAAL
                     KLFGFKDDVIGKQFASFCILYNAKTGIPYEDPVTQVLRQGKPVSLYRNTMIRNNGDTY
                     YVYIVCNPLYIANKIAGCIVVIRDITDLVRYEDEMIKTAKYESLSIFAGGIAHDFNNM
                     LNAILGNISIIEHLSPENERIIKVAGRAQKAIFKARDLTTQLLAFSKGGAPVKTSASL
                     VNIIKDTAEFVLSGSAIQCRFFISDDLHNALIDPAQISQVIHNIILNARQAIGHQGII
                     TITANNVVVNDPSLPISGEFIEITIHNTGETIPPDIMPYIFDPFFTTRKDGHGLGLSI
                     VYSIIKRHDGHITVESSNEGTCFTLYLPKAESIYKKDQNQPDTGYLTSQMSILIMDDD
                     ETVTESLKEMLSLQGCHVISVRDGDEAVKAALQAVKQNKPFDIAILDLTIKGGKGGVD
                     SVRELKAILPCCKFIASTGYANEPILANYKEYGFDAILPKPYTFNELIKVLSSLGNNQ
                     "
     gene            complement(20152..20823)
                     /locus_tag="PLJ75_06310"
     CDS             complement(20152..20823)
                     /locus_tag="PLJ75_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPD04867.1"
                     /translation="MKKHINDMCGRFAQIEPIDSIAQRLGIDEVLIDTIAPRYNISPG
                     EDVVALVHTKKKSLVLLRWGFIPYWVRDISRVKPLINARGETVAVKPGFKKAFFNNRC
                     IILASGFYEWKKEGSKKIPYYITIDDFFGFGGIFDTAIIGGKELRTCAILTTEANDAM
                     HVIHSRMPLIIRQKEFELWIKSETPLDILKELIKPFSGNCIMHPVTTKVNNPAYNDPD
                     CINPV"
     gene            complement(20804..>21672)
                     /locus_tag="PLJ75_06315"
     CDS             complement(20804..>21672)
                     /locus_tag="PLJ75_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HPD04868.1"
                     /translation="IAKLLKDRNVQLEYVLDEGGAIMEGALPGISNPIAMVGIAEKGY
                     LTLKLTVKGTGGHSSMPPHHTALGVLARALVAIEDNPFPKEIKGPAQQMFEYVTPHMS
                     FPLKVMFANMWCFKPVLKWQFEKAESSNAIMRTTVALTMAGASEKDNILPTQAWAMIN
                     CRILPGETMESTTAYIKKVISNEAVLIESTPWSNDPSPVSDINAPSFHSIATITRQLD
                     NSIIVAPYLVLGATDSRHYNALTNQIYRFTPVKMNNEDLKRTHGINERISISDMGKSV
                     KFFIALIEKTHQ"
BASE COUNT         6560 a         4668 c         3754 g         6690 t
ORIGIN      
        1 ggaacggatg aacacggtga taaaattgtt aaggctgcac tgcacaacaa tacccggcct
       61 caggaatata ctgataaaat aagcgcaata tttaaagagc agtggaaatc catggggatt
      121 gagtttaacg attttatccg cactactgaa gagcgccata aaaaagtagt acagtatgtt
      181 ttacaaaagg tgtatgataa tggtgatatc tattttggaa gctatggagg atattactgc
      241 tttggctgcg aacgctactt taccgaaaaa gagatggttg atggaaaatg ccccgatcat
      301 cagacaaaac ttgaatatat tcacgaagaa aattatttct ttaaaatgaa taaatatcag
      361 cagtggctta ttgactatat ccatgaacat cctgatttta tacgccctga acgctataaa
      421 aacgaagtgc ttgccatgct tgaaggcgaa gcactggaag atttgtgcat atcgcgtcct
      481 aaagcgcgtc tttcatgggg tataccgctt ccctttgata ccaattttgt tacctatgta
      541 tggtttgatg cattaataaa ttacatcagc gccattggtt atcctgacag tgatcttttt
      601 aaaaaattct ggccggtttc aaatcacctc attgccaaag atattgtaaa gcctcatggc
      661 attttctggc caacaatgct aaaagctgct ggtattgaac catatcagca tctcaatgta
      721 cacggctact ggaatatgga aagcacaaag atgtcaaaat cattagggaa tgttgtaaca
      781 ccatcacagc ttctggaaaa atatggcaat gatcagatac gatatttctt tttacgcgag
      841 atgacctttg gtcttgatgc aaaattttct gaagaagcta tcaccaacag gattaactat
      901 gatcttgcca atgatttagg gaatctcatt aaccgaacct tcaatatggt tcaaaagtat
      961 tgtgatgcta aagtgcctcc ctttcatagt gatgaacctg ctaaccgcga tgacatgcaa
     1021 caagcattac aggtgtctat gcatcaatac attgactttg tgcatcaatt ccagtttaat
     1081 atagcattag aacgattatg ggattttgtc aggtactgca ataaatatat tgacgagagt
     1141 aagccatggc aggttgcaaa ggaaaataat gttaagaaac tacaatcaat attgcgcaat
     1201 cttttagaag gtatccatgc tgttgccatc ctgctatctc ctgtttgcat aacaaaatct
     1261 tcgttagtca taaaggcact gaaatcatca gcacaatgca ccatagatac tttacctgcc
     1321 atgtcatcat tatccgatgg cactacttta gattcaccag gtattctttt ccccaggtta
     1381 gaaaaagaaa ctgaagctgt aaacagtgca cacagtacaa aaaaagaggc atttatggaa
     1441 acaactcctg aaggcttgat tgatatcaaa gactttttga aggttgaatt gcgaattgct
     1501 aaagttttac aggcaaaggc tgtggaaggt tcatcaaaac ttattgaact gtcaatagat
     1561 accggtaaag atattcgaac catcatagcc gggcttgcac cacactacac ccctgactat
     1621 ttaactggca aaaagattat tgttgtggca aatcttaaac ctgcaactct ccttaaaaag
     1681 acctcaaacg ggatgctttt agcagcaaaa ctttcaaaag aggatgctcc cattttaatt
     1741 gaaatagacg ataaggttcc tgttggtgct gtgctgagct aactggcata gcacatatta
     1801 gaggttacat gaaaattgga ttgatatccg acacacacaa tgatattgaa ttggttcaga
     1861 aagcaatagc actatttaaa gaacgagata tatccatgat tgtccatgca ggcgacctga
     1921 catccgccag gattatatca ttatttaaag ggtttcaggg caggtttgtc atgggaaatt
     1981 gtgatattga tgttgaagag attaacaaaa ccgcacgtga ttgtggattt ggttgcgttg
     2041 attatgcatg cgaatttact gtagagggaa aacatttcat ggtatttcat ggcaacaatg
     2101 ttcccatgtt cagagaggca gtcaactctg gtaaatatga ttacattatt aaagggcata
     2161 cccatatctt tgaaaattac ctttcgggaa aaactcgcat tataaatccc ggcgcactat
     2221 atcgcggtga agagcttacc gtcgccatac ttgatactgc aacagataag gttgaaaaaa
     2281 tccgcatcga cattgaatag ttaccgctcg ccttttatga tacttacaat cctatcaaaa
     2341 tcttctaatg agtaaaagct tatctctatt ctgcccttcc cggcgctatg gtgtattgca
     2401 acacgcgtcc ctaaaaaaga aataagctcc tcttccaatt tccgcagatg tgcatccttt
     2461 gccttcttct tttttttgat actacttttt gaaacatcgg cgatagctct ctcaacatca
     2521 cgtaccgaca gcccctgatt aataattttt tcataaagcg cttcctgctc ttccatggat
     2581 acgcttaaca gcaactttgc atgtccaaca tttatttttc catcctgaag cgcttcctgc
     2641 atatgttcgg gaagattaag caggcgtaaa atattggcaa ctgttgcacg gtctttccca
     2701 accctgtcag aaatttctga ttgttttaat ttaaaccgtg tggacagtat cttatatgct
     2761 tttgcttctt caatggggtc taaattggtg cgctgtatat tttctataag cgccagggta
     2821 aggttttgtt cttcggaggt attcatgaca atagctctga tggtgggctt ccccagaagt
     2881 ttacatgcac gccacctgcg ttcaccagca acgatgaaat actcatcgcc ctgtttgcgc
     2941 acaatgattg gctgcagcag cccaacatgc tgaatggact gtgcaagccc tgcaatctct
     3001 gcctgatcaa aatgcacacg cggctgttct ggatttggca ttattgatgc aaccggtata
     3061 tctacaatgc ggtctttatc ctgcaacaat accttttcaa aatcgctcgc cggtgttggc
     3121 gaagtggtaa taattgcacc caaacccttg cccaatactt ttttagccat tgttcacatc
     3181 ctcattcata ataaatgttg acatacatta tcattgccag ctttcagtag agctgcacca
     3241 tcaccagtag tacaaaatgt aaccggtcat ggcaacaggt caccgtgagc gtaaaagctc
     3301 cattgctaat tgttcatagc ttacactgcc gcttgatgac ctgtcatata aatttattgg
     3361 cttgccaaat gaaggcgctt cagaaagccg cacattcctt ggtatgatgg cagaaaaaac
     3421 tttatcatga aaaaattgtt taacatcagc aacaacctgt tgcgaaaggt tggtgcgcgg
     3481 gtcatacatg gtaagcagca caccttcaat ttccagctct ggattaaggg tgtcctgcac
     3541 cattgctata atcttaagca actgactcaa accctcaagc gcatagtatt cacactgtag
     3601 tggaataata accgaatctg aagcaacaag gctatttaac gttaaaacac ccagtgacgg
     3661 agggcaatca ataaaaataa aatcaaaggt atcccgaacc ttttgaagcg cacgcttcag
     3721 caggtactcc ttgtcatcca tgtccataag ctctacctgt gcacctgcca gattcatatt
     3781 tgagggtaca atatgtaaat tgtcgatagt tgttttataa atagcatctt cgacgggaag
     3841 ctgacctatc aggacttcat acacagtggt ttccatctcc tcaacattga cgcccagccc
     3901 aaaacctgca ttagcctggg gatcaatatc cacaatcagc acacgctttc ccttttccgc
     3961 taaaaaggca gctaaattta ctgctgtcgt ggttttccca actccccctt tttggttgga
     4021 tattgaaata atcttaccca tggcaagctc ccttataatt aattctatta ttccattaac
     4081 aggctaaaca cctgtatcaa aacatacctt tcatattgtc agattgcatc cacacagtga
     4141 acatcgcact gtgacttatt ccttatatat acatccgtca atgttatttt ataccggatt
     4201 tggaatccgc atccctacca taaatctatg atactataat ttcaaaaagt ctatacaaat
     4261 gtacacatcc gtcatttcag aatgcaagct ttttcctcag ctacaaaaaa tattatttct
     4321 gtttattcag gatatgacca tctctaaacc agtatctgcg cttaccggta aaacgacgtg
     4381 gatttaatct cttccaggta agaccaatta ccataactat cgctaaagca cctgcactta
     4441 ccttaaccac cggattgtag ggcaccatac gtttgccttt agctataaca agcgaaaatg
     4501 gctggtaagg aattcgtgcc gcctgatata cactgacaat gacctcacgc ggggttgcat
     4561 tgtaaaacgt gatttgtttt tgctgtccgc tatcccatga acctattatc gttttttctg
     4621 caacaatatc cacctgtaag gagcagtcag aagggagata aatggtagca gttcctcgtg
     4681 gcaatgtaca ccgatagctc ctggcatatt catttgatgt aaacgtatcg acaactgaaa
     4741 acaccacctt ttgcccccag taccatgcat ggtgaaacat ttcatcaagc aaagcaccat
     4801 acgccaccct gaaaataaag tagcgcatat acaggctgtc tatctccgaa tgatataccg
     4861 agtaatatac ccgccctttc ccatactgtt tgtagaaggc taacggtcct tcacgctcgc
     4921 cctgcacagt ggggaaataa ccttttgcca gtatcacagc atcagcattt tctaatgcaa
     4981 cccatccatt atagctgacc ggaagctgta tatcatttct cataacaaag gaagcaaaat
     5041 catcattcaa aaaacaatgg atctgaccgc ctgtaccaaa atgggggaag ttgttaaaaa
     5101 atgacagcga gcatcccatt gcttcaacaa actgatacga atatcctgct gcatacagtc
     5161 gtccaccatt gttgacaaaa tcctgcaata aagcgtttaa tcgtttatag tcaatacccg
     5221 cataatcatc ttttaatgac actcccttaa tacgctgtcc atgagccata acctgtatac
     5281 ttgtttctgg tggctgctct acaccacagg gaataaacac tgcatcaaat cctgagaggc
     5341 tatcgtttgc cactgcccat ccacgcttta caatgtatgg tatatggtac ttatcaagat
     5401 gtacggtaag attatcatga tacgacttca atacaagcac gcgtggtgca gcggataatg
     5461 gcaatgatag caatagcatc aacaataaca gcgctatcct catgcaagcc cccgtatctg
     5521 cgttaaaatc cttgttcgct catctaaatg accatagagc ctgtcattta aacaaaggtg
     5581 agcaacctgc aaacaaaagg ctgtcatctg tgggaataca ccatcgcgca gtgtagcatg
     5641 aatgacaggt ttgtgcagta ataattttga catagccctt gattttgtaa atccactgat
     5701 caaacaatca gaaaatggta taggggttgc ttcatcatcg tcaaagggta acacgcctga
     5761 agttcgtgca gaaaatgctg ttcgcatgat tacatcattg ctccccggcg ctccgttcaa
     5821 tatccagttg cggtgacggt tatacagcat aaacaattca atccccatat cataaaacat
     5881 atcaccaatc acagtcataa accgttcaat ggggtataat gactttttct ttttgtttat
     5941 cccttcaatt aattcatcac gggtaacatg gaatgtaggg ttgttagttc tgaatatatt
     6001 tatctcggaa agaatctctg caaagcgtgc aaactcgtgc ataaaatatg actcggtaaa
     6061 aatgcatcct tcatactgat tattaccatt ttcaaaatag agcatgctac catcaagcat
     6121 actgccatat tcatttataa atccaagcag catcccttcc agcagcatca caatatcttt
     6181 gctgtatgta tcttcataca gctcatttcg tttatcaact atcttacgct ggtcatcaaa
     6241 aacctgaatc ataacctctt tgccttcagg ggcgtacgtc aaaaacctga aaagctcatc
     6301 atagggattt aactttttat gtaaaacctc ttttgctttt ctctttaacg attccgcatc
     6361 tttacctgct tttacaagct catcatgatc aaaattccat gcagtatccg aaaccttggg
     6421 agcgcttaca taaaacatgt tgattatatc agaaagtgca acaggcttac cggttataag
     6481 agcttcaacg catgcaacac aaaacgattc aaatgagggc aatgacgttt ccttttttaa
     6541 aattgcaatc aatgaacagt cattggtaat tgccggatat aaaaaacagc gtatggtggt
     6601 aacaaaatca tagatgtact gttcaataga taaattcttt ttatttatat tttccggttt
     6661 tttttgcaaa tcttcactct cacgcatttt ctgatcaata agctggatga ttgcttcctt
     6721 tgttcctgta tcaagaagcg aaagcttaag caacgactca atatagggcg atagtaactg
     6781 tttatattct tcaggtcctg tccacttcaa ttccatatag tgtttattca gcaaacacac
     6841 tgattctaaa gcattgtaca gcaccacaca gtaattgaat ataacaggat tgtcgattga
     6901 ctgatcatct acaatatctg ttaaaatttt gtacagacgc tggcgcagtg cagattgcag
     6961 atcaaataac cgctggctta cttttaaaaa tttttgcgat ggaataagcc gccgtaaaaa
     7021 gcctatacca aacttgttgt ttgcatgcac cagcaggcgt tttttaaata gtctggtatg
     7081 tttatattta tggtacaaaa catttgtatt ttttgaatca tattgtattg tttttttaaa
     7141 ttcatccgcg cccttaactt caactttgcc gggcgatacc ggcataacag gcttatcagg
     7201 atagttttta acgagatcaa taattgctct gtacttttct tcttctaata ctcgatcatt
     7261 ggcaagcaaa gcacgtacaa tatccatttt gcctgcagga atatcctttg atagtaacag
     7321 tgctttagct ttacgtttta acgcttttag atcgttttcc atatactatg tctattcaaa
     7381 aatttgcttt tacattacaa ataatagatt acgctgtcaa gattaataca ttactggagg
     7441 atgttgggtt ttggcaggca agaaaatcca tgccaagagc cagtaccatg cacgtttatt
     7501 attttggtgc gtagtatgtt gtctaactgc aacataataa agtatttatt atcatcttgc
     7561 caatgcaacc atgggtgatt atatactcaa ttaatttcca attatagaca ccaaaccccg
     7621 caataacctc atctgcatct ggataggggc atttagacta cataccactt tttaaagtat
     7681 tttaaagttt tattttcgtc caaaatcaat aaagaatcct ctggttgcaa aatttaaaaa
     7741 atcctcttca ttaatctttt tatcattgtc aacaataacc gaagaatgat acacatacct
     7801 aatgttgttg aatgtcttag ttgagtagta attgatatgt ttaacatttt taaaggtatc
     7861 atccttataa atgatagtcg gatcaacaac cgttcgtagt actaaatctt tattatattg
     7921 tatgagcttt gcatcaataa ctccacattt tgatttgttt tcagtgtata ttttcataag
     7981 gtctgacaga aattcttcag ttgttttatt attgttttca tgaatttgta gtgaaatatt
     8041 tattgttcca caattgccaa taatggcata tttgtcattg atatcaaatt cccattgatt
     8101 gttatatggg aatattattg catattgaag tatataattt gtaacaataa taagatcttt
     8161 tgtttcattt gtcattattg ttatgccggt tttctcatct ataatagttt gatctttaaa
     8221 aatatacttt ttctggtagc tatacaaact attgcccata aaataaatag aaaaaaacgc
     8281 cacaatcagg attgtagtaa ttttcttcat cattttgttc ccctttaaaa atattttccc
     8341 aataaaggtt taatataatt tcacatgcaa ttttgcctat tccctgtagt taaaatattt
     8401 tttgctacaa ggtgccaaat tataccatgc cctattttta gtaaaaaaca aacactcaaa
     8461 cattaaataa tgcaagtaat ttttgatata tgatgaagtc acttttccag aatattacat
     8521 tgacatatct gatgacaaat attatagtga atatgcaccc taaatgttag agattattcc
     8581 acggaagaac tttataaaaa ttatccatac atttccttca aacgccaatg gtcgttaaaa
     8641 gaatgagaca ttccagatta aacagaccag aggaaaatca gaaaatctta ataaaagacg
     8701 acgtgattta atgttgcaca ttccaatagg acaaaaactc tcactagatg aaatagtatc
     8761 gctcaacgtt accatagcta taatgtataa tcggctgtca aaaagaactt taatgcgcga
     8821 tgttaaagaa ttaattgatt tagaattatt gctttttgat aacaatacgt attatgctaa
     8881 tatcgatttg ctaaagaaaa atatttcaca cagaaaaaag aagaggtgat ttttgcatct
     8941 aacattcaat tgtcttcata aaaaatgagt aaattatggg aaccatagta tacaaaccaa
     9001 ttggattaat ccattcaccc tttaatgata tacaaggaat gcccattcaa acaacaggag
     9061 tattgtatga aaaccgtaaa aaaacaaatt atcgcaattt ttattgcaac agtctggatt
     9121 agtgtttccg aatttgcacg aaatgaattt attgtaaaat cctattggat ccagcactac
     9181 aataaacttg gtctggtgtt cccttcccaa ccaattaatg gtgcagtatg gggtatctgg
     9241 tctttgtgtt ttgcaatcgc aatatttttt atagcaaaaa aattctcatt gattcaaaca
     9301 acttttctgt catggtttgc tggatttata ttaatgtggc ttgtaagctg gaatctgaat
     9361 gtgtttccaa tagcaatact accttttgcc atccctttaa gtttgctgga agcatttctg
     9421 gctagtttta ttgtaaaaaa attaacttga acctgataga cgttaacaaa agatagacag
     9481 ggttaaacat cacatgtaac ctaaaacatt caatactatt tactcataat cagaaattga
     9541 ctcctcatcc acatcatcac ttatactctg gttttatcat tttacccaaa aacaatatta
     9601 ctccggtgtt attgtccaca atagcataga taaatggacg atcagcttta aatatcttct
     9661 tttcaaaaga gaccatggat tttgtattca taacaatagc cgtaactgca gccgcctcgg
     9721 ttccttcctc atttacatca cagaatgttt tgtgaataac cttgctaata tataaatcct
     9781 ttcctccggt aatcttagaa aaatcggctt tcatttcatt aaatgcatcc tttatgccca
     9841 tctttattaa aggctcaaca agcatatacc cgcattcaac cttaaatttt ggcaaataca
     9901 cgtccacctc ttcctgtatt aaattttgac gccagctttc aaagttttta tcattcaaca
     9961 gaggcataat acttttgata tcctttttag gcaaaataac aaacatagac aaagccttcc
    10021 cctcataggg cagttcaatc atctggatat tgttattttc catatattta attgtaacat
    10081 tgcgttgcat catcatgggc gtttttattt ttttgttact atcgatataa aaattatcta
    10141 cttttgtttg atttttgtca aactgataga gccaattagc tttaaaatac acagcgttgg
    10201 taagtataag ccttgttaac ggttttacat cgtccaatgt tatggcttcg cgtattttat
    10261 cataggtatt gtttgctgcc cacgcattga tcttttttac agcctctttt cgcttatcgt
    10321 catgggtaaa atctacattg taagcctctg agtgatagta atttttcaaa atgtcagtgt
    10381 acgtctttaa taaaggataa tcctgttgaa cccagaaagc gttggcaatt gccagtttat
    10441 attttgcatt gtcaggatta agtgttttat atatcgttga aaaagcattc cacctgtctt
    10501 tgtcgtcagg aataaacgca aaaaccgaag ctatctctct ggcggtattc ccgcttgccc
    10561 cttcatacgc cataccaaat gccatagcaa tgctaaatgg cgagataaag atatttttat
    10621 ctttttgttt accatctatc tgcttaaaca gagcaaaccc cgatgcgtta ttacctgttg
    10681 ctactatggt tatgggcgat agttcctggc taaagcaata gcctgtaaaa ataaataacc
    10741 atgtaaaaaa gcttatcaat ttcattgtat atatccgcat atacgagccc tccttccaca
    10801 aaaatagatt tattgctcac agttattccg ggcttgaccc ataatccctt ttgccacctc
    10861 cgtcattctg agaaacacag tcccgttaaa tctatccttt cacaaaacaa cacgattctt
    10921 cacttcgttc agaatgacat accaccttcg ccattccggg gttgaaccag aatctctata
    10981 tgtatccaga gatcacaaaa aatcctgaaa tgaataaaac tattacatgg tccctaatca
    11041 atcatcttca tttgatatct ccggctcagg tattgggtat tcttgatctt tttctctgtc
    11101 atactcatca tcaaactgtc cacctgcttt ttctcttgct tcctcatatt tacccttatc
    11161 agcatcctct tttgcctttt gagacgattc tgcagcatcc tttgcctgat ccagagcact
    11221 ttcagcaatg caatgtgata cattgaaaca aacaccggat agcaagataa ccataataaa
    11281 aatcattatt ttcttcattg cttttcctca ttgaaattaa atagtgatta ataaaatact
    11341 gcctatcatc aaaaaaatac actgtcaagt tgaaaatcat tataccacta tatcgtgata
    11401 tgctaactct ttaaatatca tcatttctta cttttccgga ctgtacgttt gcctgacctt
    11461 aaacgtgatt ttttccctcg tttggttata ttataaggga accactctct attttttcca
    11521 caaaaattct cattccccat tgaaacttta tgattgtcat tctgagaaac acagtcccgt
    11581 taaatctatc ctttcacaaa acaacacgat tcttcacttc gttcagaatg acatgttatt
    11641 ccactaccat atcgttgtca ttacttcgta ctaaattaga tttcatacac tgttaaaaaa
    11701 gttattccac taccatatcg ctgtcattac cccaacagca gtatccgtta tacaaacaaa
    11761 aagaatttgc taaaaagctt ttcccttgta taccactacc atatcgctgt tttacatcaa
    11821 caccagtaca gtctatacaa acaaatgaga ttttgccaaa actttttcct ctattgcatc
    11881 atcgtatcgc tgtcattcca acagcagcgc cactataaaa taaaaggggt tttgacaaaa
    11941 accccttttc cctattttac catattatcc ccgctgtcac cccaacagcc gcaccacttt
    12001 ttaaataata gataatgggc acgtcaatct taaccgctcc aaaatttaaa ccaatccctg
    12061 tcataatacg cgcccgtgcc cacgatggct ttttatccag tgttttaccg tcaatggtaa
    12121 ccttgcccgg agtaacgata tactcatttt caggtaacgg agtaccacca taagcaatgt
    12181 ttttaaccgt cgcatcagac accgattgta actccagatc ggcattacca cttaccagat
    12241 caatgccaac acccagattg atgttgaaga gccatagaaa ttgtaaagcg gtattgatct
    12301 caaagggaac gcttaatgta tacatatcaa gcgtaatgtc aaatgaaggg tcaacaacga
    12361 cactgccagt aacgtcggta cctgtaccag ggtccactgt aaaggaatct gtctttgagt
    12421 ctaaatcaac ccggtatgag acatcggttg tttgatatac caagcctgtg ccaatggtca
    12481 ggccacgcca gcgcaataag ccagtgcctg cttcatcaaa caggctatag gtaatgccgc
    12541 caccaataat catggtatca cagctgaagt cgtcatcatc atatacccag gtactgtcaa
    12601 acttaccaaa ttttacattg atataaagcc ccggaataat catccctgcg tttatcccta
    12661 cattaacggc tgcactccct gcaacaccag catataaatc ccctttgttt tctatatcat
    12721 cctcaatctt gccattggta taataatcaa cattggtagt aggtgcctga gcgccaacca
    12781 taacgccggt ggtgatggca aacaggctat agttgggata gccctgctgt gtggcggcat
    12841 tggttgaata catgttggca ttggcaaaac cagtagccag tttatcctga tcatcatact
    12901 ttgccaattc ggcattggca tcaagtatgg cttgattaaa ttctgttgat acatcaattg
    12961 tgttccatga tccacctaca tatacacgta attgtggtgc agtacctgta atggttgctg
    13021 caaacagtgg ctgtgcaata accacacctg ctataaccag cactacaata agctttttca
    13081 taagtccccc ttgcaataaa atatttgcca tcgtcactca gaagaagcaa agcaacacaa
    13141 gaatatgtcg ttttagcaaa tagattcttc gccttcggct caggatgaca tacccaccat
    13201 tgtcatttta ataaacaccg tccccaaaga atctattctt ttaatacaac aaaacaagat
    13261 tctttgcttt gactgtcctc gccaccggca ttatcgatta agagtgacta tcgcccatgg
    13321 caaacaccat tcattacttc atcttccatt atagttactt tagacatacc tgctttgctt
    13381 tacaaagtgc aaaatttttt atatcattat ttgttaatca tatacccata catccggtat
    13441 atcgtcaaat ggtatcacct caatggtatt ttttacttta tatttttttt gacccggata
    13501 tactatccat gcctttttta actgcaaatc ctgtactgca ttatgcattg attttgtcag
    13561 caccggtgca tcagaaaatt taaactcaca accccatgca tatccaccat gttgccatac
    13621 catatccagt tcagcaccac tgtggacacc ataaaagaaa atttcatcat cattcttttt
    13681 tattgagcgc acaaccgctt caagtgcaaa tccttcccat gaggctccta actttggatg
    13741 gcgcaacaac gcgtccttgc tttctatcat aagaaggcta tgcagtatac cggaatctct
    13801 gatatataat tttggacgtt ttaccaaacg cttcccaatg ttggcatacc acggctgtaa
    13861 taggcgaacc ataaaggtac ctgctaaaat ctcaatataa cgcctgacct gcgtatccga
    13921 aatctctaat gacctgccta tctctgaaaa attaacaaca tttgcatgat aatgtgccag
    13981 catctgccag aatcgcaaca aggtataggg cggaatacgt atgccaagca atggaatatc
    14041 tcgttctaaa aatgttttaa cgtaatcttt acgccacata acacttgtag tatcatccgg
    14101 agctaacaat gaacgaggca atccacctct gaagtgaaaa tgctccagca aactgtgtgg
    14161 taaatcttct ttacgaaatc ccatgagcac ataaaatgat attcttcccg ccaatgactc
    14221 cgatgattgt ttgattatat caggagaagc acttcctaat atacaaaacc tgcattcagg
    14281 atttttatcg cacagatatc gcaataccgg gaaaagttct ggttttagct gtatttcatc
    14341 tatgattatc aatccctttt ctttatcaag ttgtaaaaca ggattttcaa gggcagccat
    14401 atccacagga ttttccagat caaatatcat atcaggtcgt aacatccctg ccaatgttgt
    14461 tttacctgat tgccgtggac caataatggc tgttacaggg taatagcgta tgcgtttttg
    14521 tatttcttca atatcatatt ttctttctaa aatcatcgtt tccttgaaat ttctttatga
    14581 gttgctgatt tatcatggta ttctacccgg gtattggata cctgtcaacc aataatcgca
    14641 ttatccgcgt tttggaattg gcaaaaccat gttacctata aactatctat cgcaacccta
    14701 tttgtatttt tttaaccgtg cattgatcat tgtgtacagg atcacaagcc caacaaaaag
    14761 aatgctcaca atgatacagg gtatagtata gcttttgagg tgcaatgttt ttgttgccca
    14821 cgacggcagg atactataac atagcgcaaa aatgagcagc acacccaata gcttccattt
    14881 gtccctgatt gcaaacgggc tcataacaat tttaaatagc accaccgtcc acaaaagaac
    14941 tgtggcaata acaaccaata ttattatgac ggttatatca atcatagcaa atccctttat
    15001 gcgttattct ggtgatattc attaaaccca tgatttttta aatacatcac aattgcgtca
    15061 cggtatttca taaaatcttc tctatgcttt tttaatattg ctataactat ttcggcatta
    15121 acattactgt attgatgaac cacaacatcc ctgaatttta ccattttgtt cattgtatta
    15181 aataaatcat ctgatactta tagtattttc atgtaatacc ttaaaagtat cagcatagct
    15241 ttccggaata cgtaacttat tatcagcaat aatatgattt gctatatcaa cacaggtttc
    15301 tatcattatc tgcaatgtcc gttcaattat tcgctgtatc ttccagtcgt tgctatattc
    15361 attcaatgta acacctttaa actcttctat ctgtttataa tattcttcca gcttcgaaag
    15421 ctttttaaga attaatggta tatctaccaa tgttatacct cctgaataaa atctgctcct
    15481 ctttcatttt aaaatcaaaa tatttcctca atgtcagtga ttcatatata tatcgcttta
    15541 atggctcctt atctgttaag attactttat tcaatactat tctccccgta atgctctccg
    15601 gtgcagtatt taatataaca atatcaatct cagatgtttt tacggcttct gcaagtttgc
    15661 aaaaaagatt gaatgtatag tcagcaacat ccggtacgtc atcaatataa atagcaatat
    15721 caatatccga aagaggtgtg taaaaaccac gtgctaatcc accaaaaaga tatgcaaaaa
    15781 tgatagcttc atcctgcaat atctttgtta tatcaggaat gtatgacatt actgtgtcag
    15841 gaataggttc gtagtgcatc ataactataa actttacaat aaactatcta tcgcagtctc
    15901 aatgacatgg gtaaataatg ccacattttt gcttattgtc aacaacaata ccgttatcac
    15961 ctgacaagcg gtatctcatc ccagtgcgtt gtatagcgcg gggacagata ccgccggcgc
    16021 attgcccatt gccgctgcac accctctgaa gcaaaataca ccgtgccacg gccacgtgcg
    16081 gcattaacgc tgtcaagcgt ttgcataagg ttgtagcgtt tccggcagct ttgcgcaggt
    16141 gaaaaaagtg tgggcgatag ttcctgtgca tcaacgatat ccgtaagcat gataccaacc
    16201 tttttatagc gatagccgct ccggaaaagc ctgtgcaaag cggtttgtgc cgcagcaatg
    16261 atatccggcg tataacacga gtgcatatcc agcgtaatgg tggcagcact gctgtactgt
    16321 ggttcatcct taaacgggtt tgttgacaca aacaccgtga caacgcccgc tgcaccatgt
    16381 tgcttccgca acttctgcga tgctatggat gcatagtgcg ccgccgcctc ctgcaactca
    16441 ttacattccg ttaccggttt gccaaatgag cgcgagctta caatgcactt ttttggcggc
    16501 ggcgcatcct ctgtgctgat gcagggaatg ccccgcaatt catacacggt tctgagtccc
    16561 acaatggtca tggtcttttt tataaaactg tcatcagcgc atataagctg ccataccgtg
    16621 tctatgccat gtctgcgtaa catacgtgcg tagcgcccgc ctatgcccca tacctcctgt
    16681 acatcaatgt gcaccaataa atttttatac agggaactat cgcccacaaa aacaaacaca
    16741 ccgctttgtg tgcgtttttt tgcaatatgg tttgccagct ttgccagcgt tctggtggcg
    16801 cccatgccca ccgaaacagg catccctgtc cattgcagaa ccgtatgctt tacctgcagc
    16861 gcatagcctt ctgcatggca cggtgggaca ttgcacagca aaaacgcctc gtcaatggaa
    16921 tacacctcca tatacggcgc acaggttgca agcgtatcca ttacccgccg agacatgtcg
    16981 ccatacagcg catagttaga cgaaaacact gcaacatggt gtttgcggat gatatcagtg
    17041 cactggtaaa atggcgctcc cattgcaatg ccaagcgctt ttgcctcagg gcttcgcgct
    17101 atgacattgc catcgttatt cgacagtacc accaccgctt taccccacag ggcggggttg
    17161 cacacctttt cgcatgaggc atagaagtta ttgcagtcaa caagcgcgta cataatcagc
    17221 caaccgtccg gatgatatga ataacaacac cccatatggt acaatccatc tcaggggtca
    17281 gagccatagc ttcataggtg tcattgtcgg gcacaagcag ggcggcgccg ttttttatga
    17341 caagacgctt tacggtaagc tcgccgttaa ctgcggcaat gacaatattg ttgtgcaccg
    17401 gggtcagagc cctgtccacc acaaggatat cgccgtggca tatccccggt gtcatggaat
    17461 cccctgacac acgcacaaaa aaggttgcag caggatgccg tataagatat tcgtttaaat
    17521 ccagcaccgt ttctatataa tcgtccgccg gcgatgggaa tcctgccttg atggctgttg
    17581 catagagcgg tgcgataggc gagcgcgatg aacaattgat tactggtaat gggcacatga
    17641 caatactcca atcatatata tgtatcctga tgttacggtt gttcctttca caatccatag
    17701 cgtgcaactg caatactacc gcacactgcc agtgcgatag caacaatgat atactataca
    17761 taaattaagt caacacaagt tttgtatttt actgaaaaaa tttttaccga taactatcgc
    17821 ccggcattta aaaatactaa acaataacag acaaccatcc catgattgaa caaagttgta
    17881 atgttttgca aagaacatgc cgttaaaaaa tgagaggtgc cagcatgaat tcccctgata
    17941 gatatttttt acaaccatac caaaataacg atattgtgtt gcgcctgaaa gcgtgggcag
    18001 cgctcatctt taatttttca gttatttttg ctgtagtaat aatgggaacc ttttttgctg
    18061 ttcgcggcat aaaggacccc ggcatccttt acccctttac gggtaccatt ttttctgcaa
    18121 ttatttcatt agcattgctt cgcaaaggaa aatttcaatt tgcggctaac ctctcatttg
    18181 caagtgtgca gatagcgttg tggcttgtta tattttttga gtccgggtct ctgctgcaac
    18241 gtatggattc cattgtgctg atttctgcaa gccttgccta tacaccgctt ctttttgttg
    18301 atcgaagacg aggtatatgg ttttactata ctgcaaatat gcttttgctt attatatttg
    18361 tattgtatgt acaagccgcg ttggggcttg aacgatggga agttattgaa tattttatgg
    18421 attctgcatt agcattaaca tttgtaactg ccatctcgta tcaaatcttt tcaattcata
    18481 agctgggtat catcaatgca tataaagctc aaaataagat acagaagcag tatgaagagc
    18541 tgtcggctac agccgaagaa ttagaagcta tgaatgagga attaacggca ataaatgaag
    18601 agcttgaaaa tactcagcag gagcttttga taagcaatag caatcttcaa aaagaaactg
    18661 cacagcttca aaccattctt tcttcaatta ctgacggaat aatcacaact gatgataagg
    18721 gaactatcac cagtataaac tatgccgcat taaaactttt tggctttaag gacgatgtta
    18781 ttggaaaaca atttgccagt ttttgcatcc tgtacaatgc aaaaacagga attccctatg
    18841 aagacccggt tactcaggtg ctgcgacagg ggaagcctgt ttctttgtac cgcaacacca
    18901 tgatacgtaa caatggtgat acctattatg tttatattgt ttgcaatcct ctctatattg
    18961 ccaataaaat tgcagggtgc attgttgtta tacgtgatat caccgatctt gtccgctatg
    19021 aagatgaaat gataaaaaca gcaaaatatg agtcgctttc tatttttgca ggaggaattg
    19081 ctcatgactt taacaacatg ctcaatgcaa tccttggtaa tatttcaatt attgaacacc
    19141 tttctcccga aaatgaacgt attattaagg tagccggacg agcacaaaaa gcaatattta
    19201 aagcacgtga tttaacaaca cagttactgg ctttttcaaa aggtggggct cctgttaaga
    19261 catcagcatc acttgtcaat attatcaaag atacagccga attcgtgctc agtggttccg
    19321 caatacaatg ccgtttcttt atatcagatg atttacataa tgcattgatt gatcctgcac
    19381 agatctcaca ggttatccat aatatcatcc tcaatgctcg tcaggcaatt ggacatcagg
    19441 gcataattac cattacggct aacaatgtag tggtaaacga cccttcattg ccaatcagcg
    19501 gtgaattcat tgagataacg atacacaata ccggtgaaac aataccgccc gatattatgc
    19561 cctatatctt tgatccattt tttacgacac gtaaagacgg acatgggctg ggattatcca
    19621 ttgtttattc cattattaaa agacatgatg ggcatatcac ggtagaatcg tcaaatgaag
    19681 gaacatgttt tacactctat ttaccaaaag ctgaatcaat atataagaaa gatcaaaatc
    19741 aacctgatac cggctattta acatcgcaaa tgtctatttt gataatggat gatgatgaaa
    19801 cggtaaccga gtcacttaag gaaatgcttt cccttcaggg ttgccatgta ataagcgtca
    19861 gggatggtga tgaagcagta aaagcagcat tacaagctgt aaagcaaaac aaaccttttg
    19921 atattgctat tcttgatcta accattaagg ggggtaaagg tggtgtcgat agtgtccgtg
    19981 aattaaaggc aatattacca tgctgtaagt ttatcgcatc aaccggatat gctaacgaac
    20041 caatattagc taactataaa gaatatggct ttgatgcaat actgccaaaa ccgtatacct
    20101 tcaatgaact catcaaggta ctgtcttcac ttggcaataa ccaataatct gttatacagg
    20161 atttatgcaa tcaggatcat tatacgcagg gttattaacc tttgttgtaa ccggatgcat
    20221 gatacaatta ccactaaagg gttttatcaa ttcttttaat atgtccagtg gcgtttcaga
    20281 ttttatccag agttcaaatt ctttctgccg tattataaga ggcatcctgc tatggatcac
    20341 atgcattgca tcatttgctt cagtggtaag tatagcacat gtacgtagct ccttgcctcc
    20401 aatgatagca gtatcaaata tccctccaaa accaaaaaaa tcatctatcg ttatatagta
    20461 tggtattttt ttactgcctt cctttttcca ctcatagaag ccgcttgcca gtataatgca
    20521 cctgttattg aaaaacgcct ttttaaaacc tggctttaca gcaactgttt caccgcgtgc
    20581 attaatgagt ggttttactc tggatatatc ccttacccaa taggggatga acccccatcg
    20641 caatagcact aacgattttt tcttagtatg caccagcgct acaacatcct cacctgggct
    20701 gatgttgtag cgcggtgcga tagtatctat taatacttca tcaattccta aacgctgtgc
    20761 aatactgtca ataggctcaa tctgtgcaaa tcgcccgcac atatcattga tgtgtttttt
    20821 caattagtgc aataaagaat ttaacgcttt tacccatatc agaaatactt atacgctcat
    20881 tgatgccatg ggtgcgtttt aaatcttcat tattcatctt tacaggtgta aaccggtaaa
    20941 tctgatttgt taaagcatta taatgtcttg aatcagttgc ccccaatact aaataaggtg
    21001 ctacaatgat agaattatct aactgtcggg tgatagttgc tatggaatga aatgatggcg
    21061 cattgatatc cgataccggt gaaggatcat ttgaccatgg tgttgactca ataagtactg
    21121 cttcattact gataaccttc tttatgtatg cagtggtact ttccattgtt tcaccgggca
    21181 ggattcggca atttatcatt gcccatgcct gtgttggtaa tatgttatcc ttttcgcttg
    21241 cacctgccat ggtaagtgca acagtggtac gcattattgc attcgatgat tcggcctttt
    21301 caaactgcca ctttaatact ggcttaaaac accacatatt tgcaaacata actttcaaag
    21361 gaaaactcat atggggagta acatactcaa acatctgctg tgccgggcct ttaatttctt
    21421 ttgggaaggg attgtcttca atagcaacca atgctctggc aagcaccccc aatgcagtat
    21481 ggtgaggtgg catggatgag tgcccaccgg taccttttac cgtcaacttc aatgttaaat
    21541 atcctttttc ggcaatccct accatggcta taggatttga tatccccggc agcgcccctt
    21601 ccatgatagc accaccctca tccagaacat attccagttg aacattacga tcttttaaaa
    21661 gtttagcaat ag
//
LOCUS       DAOOJH010000034        21218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2501_length_21218_cov_9.168029, whole genome shotgun sequence.
ACCESSION   DAOOJH010000034 DAOOJH010000000
VERSION     DAOOJH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21218
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2501_length_21218_cov_9.168029"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..509
                     /locus_tag="PLJ75_06320"
     CDS             156..509
                     /locus_tag="PLJ75_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04869.1"
                     /translation="MSNGQLTINQIKEELIPIIQAVVTQEVKTNIEEFLRQSEIKAKE
                     LSLIERVIRVEEELKALKEIEKARFEAMETRFAAIDTRFEAIEKRFTNLQWFIGIGFT
                     IISILIAVFGLFVGK"
     gene            754..1023
                     /locus_tag="PLJ75_06325"
     CDS             754..1023
                     /locus_tag="PLJ75_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04870.1"
                     /translation="MTPQNYWAFPKEIPLEEVPRYSSIFEKINEPKTIQFDLTKTEWI
                     HSSFIGFLIYAKHLAASKGGKLYIIPSPAVQHILDLMNLREYLLH"
     gene            1531..2514
                     /gene="selD"
                     /locus_tag="PLJ75_06330"
     CDS             1531..2514
                     /gene="selD"
                     /locus_tag="PLJ75_06330"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPD04871.1"
                     /translation="MGPADLDAIVKKLPITYTGNVLVGMQTGDDAGVYKISDDVAIVQ
                     TVDIITPIVDNPFVFGKIAAVNSISDIYAMGGTPITALNIVCFPVTTFTMDVLEKILL
                     GGLQTLQQTGIQLIGGHSIDDVELKYGLAVTGLIHPDAIITNTGIQPDDVIILTKPLG
                     TGTINTAIKAGVADSNHIDECISSMTTINDCMAGYPYPEHIHAMTDVTGFGLIGHLNE
                     MLGDSSLEIVINTEAIPFLNGATRYAAMGLLPGGLYRNRDFAGKRCTIHSSVKREIAD
                     LLFDPQTSGGLLIAVSPDNAGKVIDYSAKKALCTAIIANCRKADSAHISIE"
     gene            2526..2900
                     /locus_tag="PLJ75_06335"
     CDS             2526..2900
                     /locus_tag="PLJ75_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04872.1"
                     /translation="MKYSDDKRRDLIKKIFWDYEIDIESLLNHIDNGTLPKAKEQKLF
                     IRCLEHLPWHCVVGVFGFDKAKELLTDEVIQKVWPEERSDHFATLKKILRGEPLPTTR
                     WDSELRKKLKSTVLSYRWYRTQ"
     gene            2782..3477
                     /locus_tag="PLJ75_06340"
     CDS             2782..3477
                     /locus_tag="PLJ75_06340"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPD04873.1"
                     /translation="MQPLKKFYEENLYPLQDGILNCVKNLNLPFYLTGGTALSRGYFN
                     HRYSDDIDLFTNSNPDFKSHSDAIVLTLKNNTYAIDTSTMILSQDYVSFYVSCEGLSN
                     IQCKLDLVNDKAPHFGIIQPTPVYYKTDDWYNILINKFTALFRLEIKDFVDIWIIAKN
                     KKFNWDDIITKGREKELGLDAIVCAHLMKTISFELLPTIKWAIDVSFDEFLKDMDTIA
                     IDLLKGCDNSLVT"
     gene            3535..3626
                     /locus_tag="PLJ75_06345"
     tRNA            3535..3626
                     /locus_tag="PLJ75_06345"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3569..3571,aa:Sec,seq:tca)
     gene            3693..5027
                     /gene="selA"
                     /locus_tag="PLJ75_06350"
     CDS             3693..5027
                     /gene="selA"
                     /locus_tag="PLJ75_06350"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPD04874.1"
                     /translation="MYKQIPQVQKLLEEEAIQPFITTIGHPFVVSAIRDTLDMERKNI
                     ADGKPFDYASLIGAIVARCRAISLEKLQRVINGTGVLLHTNLGRAPLGKEVLQSIAQQ
                     LYGYCNLEYTIPTQKRGKRGGFAEKLICTITGAEDALIVNNNAASIFLILHHFAHGKE
                     VVISRGELIQIGGGFRIPDIMQQAGCTLVEVGTTNITRQNDYIKALTPNTAIILSAHR
                     SNFYMKGFTDSPTLQQLAALKTDSLLLVKDLGSGNFIFNDEDNVSQALNHADIVCFSG
                     DKLLGACQAGIIAGKKALIEELRNNPLMRIMRVDKITYFIIQEVLLRYANHQHCTLPV
                     WDMMKQDVTIIDKKIKKLMKKLNADIKPYIHHTASKTAFGGGAMPHKVLPDYGIIIAI
                     DGKRAGDIAHHMVHQPVPIIGYIQNDAYTLNFRTIDDNDIASIADAINSLLP"
     gene            5037..6779
                     /gene="selB"
                     /locus_tag="PLJ75_06355"
     CDS             5037..6779
                     /gene="selB"
                     /locus_tag="PLJ75_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPD04875.1"
                     /translation="MYVIGTAGHVDHGKTSLIKALTGIDADRLPEEKQRHMTIDIGFA
                     YITLPAIGMVSIVDVPGHERFIRNMVTGAWGIDIGLLVIAADDGWMKQTDDHVKVLSI
                     LGIPAMVVALTKIDTIEQQTLPQVLSHINAMLATTPYRQAPVVPCSSITGEGITALKE
                     ALTNAIIKLPVPTTGNKPYLHVDRVFTVKGAGTVVTGTSKNGSFALNDTITIVPHATK
                     ARIRQIQSHNTQQQQTDVSQRTALALAGVEKTTLQRGDIIVRENFFTSATHCIAYITH
                     TFTQIKNNQWIEVLIGTASYDAQYTMISDSKPIVRLKCKQAVWLYPSQRFVATLPGGH
                     AIIAGGYILCHNYSALIKKDPVIHAITNNNSIEANAILCIVACNPFITNHALKKLFPD
                     ESAVAKAIQLPVQQSLLMLIDDIVLTQFQYDTAVKLLKQYAGTDTTTVDIAQRLSIPH
                     NLVNHIMAQYGYGKKLNGEMSDAEKHLLQQLLQKGIEGINPKDFQRHKNILDKLIKSE
                     NAIVIDRELLWHRDVFLHYAESIMAHFNTNKELTVQQAKEITGLSRKYVIPLLNALEQ
                     KKFIKRYGDVRLKA"
     gene            6883..7908
                     /locus_tag="PLJ75_06360"
     CDS             6883..7908
                     /locus_tag="PLJ75_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD04876.1"
                     /translation="MALFTPNIDKLREKNDIQGLLKLTKHRKAEVRLNALLALVKTRD
                     QSVTQELKKLINDPDPRVRTIATIKFGDINDPAIIENLRSIIISGSQRDKIEALRVLS
                     EIGPTDDLEISKILYLAFKDKKMMVSIEAIRTMGYLKDKYAIPHLVEALDSKHYQIRV
                     EATRALGMIATEQAIDPLIGCLVDNHAEVRKAAHDALKKIGTDKALKAITDAPFMLLV
                     KHMTEGGSQREETVKQIGQYRIKEAAPLLIKACNDEFKSVRAAAARSIGILREKSAVN
                     ALTKLLNDPYYDVRLEAVRALEKIFDPQALKAIEKAMSDSNKNVRDEARVAYYSLKSR
                     LEKVYET"
     gene            7923..8690
                     /locus_tag="PLJ75_06365"
     CDS             7923..8690
                     /locus_tag="PLJ75_06365"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPD04877.1"
                     /translation="MILGFNIGNTNTVMGIYDEDNPEPVYATTYPTRQTIQSDELSHI
                     IYNTLKQWDKRLASSITGVIYSSVVPQSNTTYNKTIEELFKVTVHSITHLSKMPIRIE
                     YDNPAQLGVDRIVNAVAAFTDYACDCIIVDIGTAITFCQLTQGVLRGGIIAPGVKVAM
                     KGLAQSTAQLFEVPFEKPPYIIATNTSDALISGFFYGWIALIEGIIGKIQSTMGTCMV
                     IVTGGMAEILYPHLSISAIVDHSLTMRGIFYLYNSNR"
     gene            9306..9767
                     /locus_tag="PLJ75_06370"
     CDS             9306..9767
                     /locus_tag="PLJ75_06370"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPD04878.1"
                     /translation="MNNYERYTRILARSVDHIFKHFLYDNDIKEVFTNQSTPDDPRVT
                     IQLDGTMKGELIINFPEQTLHQLTKNFFINAKGNIKKYYPDVAGEVANLIAGTFINQL
                     QFIKHNLKPSPPEYNEEHTTLKTLYENINLSFESRFGGFDLDFYYKDLLHQ"
     gene            9767..11479
                     /locus_tag="PLJ75_06375"
     CDS             9767..11479
                     /locus_tag="PLJ75_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPD04879.1"
                     /translation="MAVIDYIIIVIYFLFSIGIAFLFSRRARTNTRQFFLSGQSLPWW
                     ITGTAMVATTFAADTPLAVTELVHSGGIAGNWLWWNMVAANVVTVFFFAKLWRRSGVL
                     TDIEFIELRYSGKEASFLRGFKALYLGLFANCIIMGWVNVALAQILAHLFNINHSYVL
                     FIVIATMLFVGLYSAIGGLWAVAVTDFVQFIIAMGGCIVLAVVVIDHPHIQGIDGLIQ
                     SIPQNALLFFPSGDSSSLPLSAFLLFIGVQWWASWYPGSEPGGGGYVAQRMMSSKDET
                     HSLKATLWFTIAHFALRPWPWIIVALATMVLYPTIPADQSKATYVFAIRDYLPAGLKG
                     MLVAAFLAAYMSTIATHLNWGSSYLVNDLYRRFIVTGKSEKHYVYSAQVCTTLLAVIA
                     SLLIFVIDSISGAWTFIIECGAGIGPVLMLRWYWWRINAWSEIIAMIAPIPAVIISRL
                     VLHIALPYNLLIIVPWTTICWVMATLVTQPVKHETLTAFYHKVQPEFGFEPIRKALHY
                     PPGNYQTCMILLFQTTLGIIATYSLLFGIGAVLLHKTGAYLYLIAFVVSFMLVIYSFR
                     MTKK"
     gene            11884..14253
                     /locus_tag="PLJ75_06380"
     CDS             11884..14253
                     /locus_tag="PLJ75_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPD04880.1"
                     /translation="MSIVEKLAQVIAHDMHLDEKTINELHDLFTISDDDTKAIEKIQE
                     IIKLSPDFWKNFYSRCLNDTALQKINSDSQFFKKHKRAIIDYFIKLPEQKIDYDYFIS
                     RFAIGIQLEKATVEPILFANILSNLYIQLIQYCEDIIHDTSLLTEIMQSYLKIVFLDL
                     TLILHAYFFMKEKKLSDAKKELERLGRSYRLIHEINDLIVRTTDENTLYENVAKILVV
                     VGNFDLAWIGMVDKQLNVVPVASYGETQYLNDIYINIDPSLPEGNGPTGTAIRDKTII
                     RINYPDEDPRFSPWKERAAKFDFKSIVAIPIIVDDNTHAIGAITLYSHNEHFVTLGEA
                     ALLQEVANDISFAIDHIRKNKKIEKILFYDELTGLGNYNYFLSALTSQIQLASSQTMQ
                     LAVVTIDIDNFTAINNSFGFNTGDAILREYRNRLQNSIDANLIARDGDEFFILYPYID
                     EMGLTSFISDLKLIATKPLIAMSHELVISYCIGIATYPNDGKTAEELLNKSRIALTEA
                     KKKGSSSICYYSEELFQNSIQKIALEKELRSALKNNEFTLYYQPKIAVASRNISGLEA
                     LLRWNHPEKGLLPPSDFIAILESSDMIIQIGKWVAAEVARQVQINPMFQEKSFIMSFN
                     VSPNQFYDSAFIFDIIKIVKSSAINPCNLEIEVTENILMSDAENVIDTCNKLQQFGIN
                     ISIDDFGTGYSSLSYLKKMPVSTLKIDASFVNGLPHNKDDVEIVKTIILLAHSLGKKT
                     IAEGVETREQLDVLVDLGVDEVQGYYFSKPMPIHECVDYCKNFNPDNYF"
     gene            14488..15480
                     /locus_tag="PLJ75_06385"
     CDS             14488..15480
                     /locus_tag="PLJ75_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04881.1"
                     /translation="MKAKAIALYSGGLDSLLAAAIVARQGVEVIGLHCILPSVDPQKP
                     PDSLAAAQLAKQIDLPLEYYRCDEQYMQVIAHPVHGYGKRANPCIDCKIFFLQKAKEY
                     MQRHNASFVITGDVVGQRPMSQYKAMLRHIEKESGLEGILLRPLSAKLLPPTVPEQKG
                     IVNRDMLYAISGRSRQQQMQLAQDSGIQDYQSPAGGCFLTMPQIASRVKDLFAHPPYS
                     TLDLYLVNIGRHYRLHDKASIIVARNEEECIILDRYKQHADFYVEPDFKGPVMYGRGT
                     ITQNDYLLLTQIMARYGRPTMTENSIALLIQGQRISNLMVDKPIGDALLNQMRI"
     gene            15502..16110
                     /gene="purN"
                     /locus_tag="PLJ75_06390"
     CDS             15502..16110
                     /gene="purN"
                     /locus_tag="PLJ75_06390"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPD04882.1"
                     /translation="MKKTVSFLVSGRGSNFQAVAKAIIKGTIPAKTGVVISDTEGVRA
                     LDIAQELSIPAFFINPGQYKSRQEHEKAMVQLLHEYSTDVVVTAGYMRLLTPYFVHEF
                     KNSIINIHPALLPAFPGTHAQRQALEYGVKISGCTTHFIDEGTDSGPIILQKAVPVYD
                     DDTEDTLSARILKEEHQLLIESVRLFCKDKIFIKGRKVYIVR"
     gene            16585..17124
                     /locus_tag="PLJ75_06395"
     CDS             16585..17124
                     /locus_tag="PLJ75_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04883.1"
                     /translation="MGLIAILLLCVFYLGLIIIIYEYYTLFSSKDEYTKQELKGVITG
                     APDHWMPLLSQFRLFPVYALASLIVGILIPLLSYNWFFQSSIFSILLFIILPQVRRSY
                     EPVKVTITDSIWDIIAAALSEYHEIMIVFFSTGTVASLTYCWISNRELPFLWFFVNAI
                     IICVIIFFTLVSIDKENNK"
     gene            17349..17795
                     /locus_tag="PLJ75_06400"
     CDS             17349..17795
                     /locus_tag="PLJ75_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04884.1"
                     /translation="MKRFLLVALIAVVAISFTACGSSKMGGQVKGDSYISEGWVTPDI
                     FRVTATGAPKPGLKDRVMRRGTAKEAAIAMAEKRIVEKFKGARLEGAAGAADYASTGV
                     AVAKEFEGLVKGGTIVKETYDEEDHCEIVYQIEKKGLKKEVEGGAY"
     gene            18001..19119
                     /locus_tag="PLJ75_06405"
     CDS             18001..19119
                     /locus_tag="PLJ75_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD04885.1"
                     /translation="MVRFKKELIEMFVQTVQLAIEKGLVNFEVLSIDSVKIRANASSK
                     KDKSIDGLNRLEDTIKEKLTRFMDDVDRDERRDEEIEALRRQEARVVEAKEELRKRIE
                     KKKEGKSGKELEKIEKNEKINLTDFDSHTMQQANGEINPAYSMTTAVDAHHDIITHFQ
                     INEEDNDGKALVPVIEGSREKCGQSHEIVAADAGFASLDNYEKLAAMNQEALIPDRRY
                     DVDRRGVQKKGAFDTYHFTYCPETNWYQCPHGIPLFFQNEYSAGGRNFFRYANRAACL
                     SCCDRTRCTKSEYRTITRDKKEGYREAMRHCLSKSENKEKYKRRSHCSESPNGNIKHN
                     LKYRIYMRRGRDKVAMEGSLLCMMHNILKMGNFSMQML"
     gene            complement(19259..20080)
                     /locus_tag="PLJ75_06410"
     CDS             complement(19259..20080)
                     /locus_tag="PLJ75_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD04886.1"
                     /translation="MSAVIIDTEKCKKDKLCVLECPMKIIILNSDTGIPETIHNAENM
                     CLDCGHCIAVCPYGALSLTSMKVEECQSISQNFNPGIEIIEYYFKSRRSIRRFKNKTV
                     EKEKLEKLLDITAYAPTGHNSRTVEFIVYTQKEEIKKLTEHVIDWMQLMISSSPDIGK
                     SMHFDMITKAWASGVDVVMHNAPVVIVAHGNKNNPNTPTSCTIALAHLELIAQAFGLG
                     CCWAGYFTWCAMAYPPLRKALSIPDDNGIYGTMLTGYPIAKYYRIPRRQALVEWR"
     gene            complement(20237..21052)
                     /locus_tag="PLJ75_06415"
     CDS             complement(20237..21052)
                     /locus_tag="PLJ75_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPD04887.1"
                     /translation="MPLYIVDAFTQEPFKGNPAAVCVLSSTISDQLMQAIAYELNCPE
                     TAFVMKQDDGFSLRWFAPLQEMDLCGHATLATAHILYQQQIINPDQMIRFYTRSGLLL
                     VKSQGDTIIMDFPKEESSECSIQQWIIDALGVKPVFTGKNRMDYLIEVESDKEVIMVS
                     PDFITLKNLDSRGVIITAQSSKNEFDFVSRFFAPGIGVNEDPVTGSAHCCLGPYWQKK
                     LHKNHFVAYQASQRGGMLKIEVKDNRVFIGGTAITVLKGEFNIQEVCYVQDNL"
BASE COUNT         6623 a         4198 c         4186 g         6211 t
ORIGIN      
        1 atggaaaaaa taatctccct acggatcccc ccttttgttc aaaggggaga acaagggggt
       61 acaaattata ttgactttta ctgtttagta gtatatagtt attctatact actttatata
      121 attataaaca ctacagacta aaggaataca ttgttatgag caacgggcaa ttaaccataa
      181 accagataaa agaagagtta atccctatta tacaggctgt ggtaacacag gaggtaaaaa
      241 ccaatatcga ggagtttttg cgtcaaagtg aaatcaaagc taaagaatta tcgctcattg
      301 agcgtgtcat ccgtgtggaa gaggaattaa aagccttaaa agagatagaa aaagcacgct
      361 ttgaggcaat ggaaactcgc tttgcagcta ttgatactcg ctttgaagca atagaaaaac
      421 gttttaccaa cctgcagtgg tttataggca ttggctttac tatcatcagc atcctcatcg
      481 ctgtgtttgg cctttttgtt gggaaataaa aaaatttttt aaaaaataaa aaaattattt
      541 gactttattg tattatttct tatgtatagt gcaattttat ttacaaaaat caatatgtgc
      601 ttttttatgt acgttagtta tgtaatgtag tataaaagta tatttttttt taattttcat
      661 agctctccct tttataaaaa attaaacttt agtacataaa aagacgatta tagatagata
      721 cggtgaagtg cgcacatgga gaggaggaag tcaatgacac cacaaaacta ctgggctttc
      781 cctaaggaga taccccttga ggaggtaccc cgctattcca gtatttttga aaagatcaac
      841 gagccaaaaa caattcaatt tgatttaacc aaaactgagt ggattcactc atcatttatt
      901 ggttttttaa tctatgcaaa acatcttgct gcatccaaag ggggcaagct ttatatcatt
      961 ccttccccgg cagttcagca tatccttgac ctgatgaacc tcagggaata tctgctccac
     1021 taacaataca taaaaaaaca cttctgcatc gactgcacat gattcaggat aaccttctac
     1081 atttcaggat gactgccgct aaatatttcc atcacccttt cggtagcttt tatacatttt
     1141 tatacattat ataaaattgc taacgttaaa caaaaaatta ccactgaatc attctgaatt
     1201 atgacaatgg cgtggtatat aacccacttc aagacaaacc aaaaacaaaa ttaagaaaac
     1261 aaacccttac taaaaatgtt gggaaaaagc attgttttat agtaactatc gcccaaaaag
     1321 tatgtgtcaa tgaaacacac acagcactgt gcgtttcata aaaattattg atacaaattt
     1381 ttcttcgggc caaacatttt tatgttgtca ctggcgctat atactatata ctgttacatg
     1441 aaggataata caatggaaag ctgtaacatc aatccaaaaa agaagttgcg ccttactgaa
     1501 tcggtacgcg ggtcagggtg agctgcaaag ataggtccgg cggaccttga tgcaatagtt
     1561 aaaaagctcc ccatcaccta taccggcaat gtgcttgtcg gcatgcagac gggcgacgat
     1621 gccggcgtgt ataaaatcag tgatgatgta gcaattgttc aaactgtgga tattatcact
     1681 cccatagtgg ataatccttt tgtctttgga aagattgcag cggttaacag catatccgat
     1741 atctacgcta tgggaggcac acccattaca gccttaaata ttgtatgctt tcccgttaca
     1801 acatttacca tggatgtgct ggaaaaaata ttactgggcg ggcttcagac gttacagcaa
     1861 accggcatac agctcattgg cggtcacagc attgatgatg ttgaacttaa atatggactg
     1921 gcggtaaccg ggcttataca tcctgatgcc ataattacca ataccggcat acagccagac
     1981 gacgttatta tccttaccaa gccgcttggc accggaacca tcaatacagc cataaaagcc
     2041 ggcgttgctg acagcaacca cattgatgaa tgtattagct ccatgactac aatcaatgat
     2101 tgcatggctg gctacccata ccctgaacat atccatgcca tgaccgatgt aaccggcttt
     2161 gggctcatag gtcatctcaa tgaaatgctt ggcgatagtt cccttgaaat tgttatcaat
     2221 acagaagcaa tcccctttct taatggggca acccgatacg ctgccatggg acttcttccc
     2281 ggcggattat accgcaacag ggattttgcc gggaaacgct gcaccatcca ttccagcgtt
     2341 aaacgtgaaa ttgccgatct gctgtttgac ccgcaaacat cgggcgggct tttgattgcc
     2401 gtatcgcctg ataatgcagg caaagtcatt gactacagtg caaaaaaagc tctgtgcaca
     2461 gcaataatag ctaactgccg caaggctgac agtgcccata tttctattga atagagtact
     2521 gaacaatgaa atattcagat gataaacgaa gagaccttat taaaaaaata ttctgggatt
     2581 atgaaataga tatagaatcc ttgctaaacc acattgacaa tggtactttg cccaaagcaa
     2641 aagaacaaaa gctatttatt cgctgtctgg aacatcttcc atggcattgc gttgtaggtg
     2701 tttttggctt tgacaaagct aaagagctat taaccgatga agtgatacaa aaagtatggc
     2761 ctgaagaaag gagtgatcat tttgcaaccc ttaaaaaaat tctacgagga gaacctctac
     2821 ccactacaag atgggattct gaattgcgta aaaaacttaa atctaccgtt ttatcttacc
     2881 ggtggtaccg cactcagtag aggatatttt aatcatcgat attctgatga tatcgattta
     2941 tttaccaaca gtaatccaga tttcaaaagc catagtgatg caatagtact tacattaaaa
     3001 aataatacat acgccattga tacttcaaca atgatactgt cgcaggatta tgtatcgttt
     3061 tatgtttcat gtgaagggtt aagcaacatt caatgtaaac ttgatttagt gaatgataaa
     3121 gcaccacatt ttggcataat ccagcctacg ccagtctatt ataaaactga tgattggtat
     3181 aatattttaa tcaataaatt taccgcacta ttccgattag agataaaaga ttttgtggat
     3241 atctggatta ttgcaaaaaa taaaaaattt aattgggatg atataatcac aaaggggcgt
     3301 gaaaaagagc tgggattaga tgcaatagta tgcgcacatt taatgaaaac aatttcattt
     3361 gaattattac ctactattaa atgggcaata gatgtttcat ttgatgaatt tttaaaagat
     3421 atggacacaa ttgctattga ccttttgaaa ggatgtgata attcacttgt tacataaaat
     3481 attttttcaa tttgtttttg acattctgtt atagtaacca tatatttttg tattggaagc
     3541 ggatgagatc tggtggtctc tgcggtcttc aaaaccgctg gctgccctta ccggcagcgg
     3601 caggttcgat tcctgcccct tccgcatttt aaaataggca aaaccttgct gcttgctcat
     3661 ctgtaaggtt ttcattgcac aggatggcag atatgtacaa acaaattcct caggtacaaa
     3721 agcttttaga agaagaagca atacagccgt tcataacaac tatcggtcac ccttttgtgg
     3781 tttctgctat tcgtgatact cttgacatgg agcggaaaaa tattgcagat ggcaagccat
     3841 ttgattatgc atcattaatt ggggcgatag ttgcccggtg cagggcaata tcccttgaaa
     3901 agttgcaacg cgtcattaat ggtaccggcg ttttgttgca caccaattta ggacgggctc
     3961 cattgggcaa agaggtactg cagagcattg ctcaacagct ttatggatac tgcaatctgg
     4021 aatataccat cccaacgcaa aaacgcggta aacgaggcgg ctttgccgaa aagcttatat
     4081 gcacgataac cggcgccgaa gatgcgctca tcgtcaacaa caatgctgcc agcatatttt
     4141 tgatacttca tcactttgca catggtaaag aggtggttat atcccgtggc gagctcatac
     4201 agattggcgg tggatttaga atccctgata tcatgcagca ggcaggttgt acccttgttg
     4261 aggtgggcac aaccaacatt acccggcaaa acgattatat aaaggctctg acccccaata
     4321 ctgcaatcat cctgtcggct caccgctcca acttttatat gaagggattt accgatagcc
     4381 ctaccctgca gcagcttgca gcattaaaaa cggattcgct gctgctggta aaggatctgg
     4441 gcagcggaaa ttttatcttt aatgatgagg acaatgtttc acaggcatta aaccatgctg
     4501 atattgtttg ttttagcggt gataagctgt taggagcatg ccaggctggc attattgccg
     4561 gcaaaaaagc attaattgaa gagttgagaa acaatccatt gatgcgcatc atgcgtgtgg
     4621 ataaaataac ctatttcatt atccaggagg tattgctacg gtatgccaat caccagcatt
     4681 gtaccctgcc ggtatgggat atgatgaaac aggatgttac tatcattgat aaaaaaataa
     4741 agaagcttat gaaaaaacta aacgctgata ttaaaccata tatacaccat accgcttcaa
     4801 aaacggcatt tggcggcggc gctatgcccc acaaggtgct tcccgattat ggtataatca
     4861 ttgctattga cggtaaacgt gccggtgata tagcacatca catggtacat cagccggtgc
     4921 ccatcattgg ctacattcaa aatgacgcgt acaccttaaa tttccgcacc atcgatgata
     4981 atgacatcgc cagcattgct gatgcaatca actcattatt gccataggta cacggtatgt
     5041 atgtcattgg aactgccgga catgttgacc atggtaaaac atcgctgatc aaggcactta
     5101 ccgggattga tgccgaccga ctccccgaag aaaagcagcg ccacatgacc atcgacattg
     5161 gttttgcata tattaccctt cctgccattg gcatggtaag tattgttgat gtgccgggac
     5221 atgaacgctt tatccgtaat atggtaaccg gagcatgggg catcgatatt gggcttctgg
     5281 ttattgctgc cgacgacggc tggatgaaac agactgatga tcatgtaaag gttttatcca
     5341 tactggggat accggcaatg gttgttgcac ttaccaaaat tgatactata gagcaacaaa
     5401 cccttccaca ggtactatcg cacataaatg ccatgcttgc taccactcct taccggcaag
     5461 cacctgttgt accctgctcg tccataacgg gagagggaat tactgctctt aaagaagcct
     5521 taaccaatgc aattataaaa ctgccagtgc ccactaccgg caacaaaccg tacctgcatg
     5581 ttgacagggt ttttaccgtc aaaggtgctg gcacggttgt tacgggcacc agcaaaaatg
     5641 gaagctttgc actcaatgat accatcacca tagtgcctca tgccacaaaa gcgcgcatac
     5701 gccagataca aagccacaat acacagcagc agcaaacgga tgtatcacag cgcaccgcac
     5761 tggcgcttgc aggtgttgaa aaaacaacct tacagcgcgg tgatatcatt gtcagggaaa
     5821 atttttttac atcagccaca cactgcatag catacattac gcatacattt acgcagatta
     5881 agaacaatca atggattgag gtattaattg gcactgcatc atacgatgct caatacacca
     5941 tgataagcga tagcaagccg atagtaaggc ttaaatgtaa gcaagcggtg tggctgtatc
     6001 cttcacaacg ttttgtggca acgttgccgg gggggcatgc tattattgca ggcggttata
     6061 tactatgtca taactattca gcgttgataa aaaaagatcc cgttatccat gcaataacaa
     6121 acaacaacag cattgaagca aatgcaattt tgtgtattgt tgcatgcaat ccttttataa
     6181 caaaccatgc gctgaaaaaa cttttccctg atgaaagtgc tgttgctaaa gcaatacagt
     6241 tacctgtaca acaatcactg ctgatgctga ttgatgacat agtgcttact cagtttcagt
     6301 atgacactgc cgtaaagctg ctgaaacaat atgccggaac tgatacaact accgtggata
     6361 tagcacaaag gctttccatt ccacacaatc tggttaacca catcatggca cagtatggct
     6421 atggcaaaaa actaaatggt gaaatgagcg atgctgaaaa gcacctgctg cagcagctat
     6481 tgcaaaaagg catagaaggc attaatccta aggactttca acgtcataaa aatatacttg
     6541 ataagcttat aaaatcagaa aacgcaattg tcattgacag ggaactgctg tggcacaggg
     6601 atgttttttt acactatgct gaaagcatca tggcacattt taacaccaat aaagaattaa
     6661 ccgtacagca ggctaaagaa ataacaggac tgtcaaggaa atatgttatt ccgctactca
     6721 atgcgctgga acaaaaaaaa ttcattaaac gctatggcga tgtacggctg aaagcataac
     6781 tatttaattt gcatgataaa ataaaattgt acattattag ttgacttttt ttgtacatgg
     6841 taactatact tgtaaaaaat aaaagtaatt aaaggaattg ctatggcact ttttactccg
     6901 aacatagaca aactaaggga aaaaaatgat attcaggggc ttttaaaatt aacaaaacac
     6961 cgcaaagcag aagtacgcct caatgcgctg cttgcactgg ttaaaacccg cgatcaatca
     7021 gttactcaag aattaaaaaa actcataaat gaccctgacc ctcgtgttag aactatcgcc
     7081 accataaaat ttggtgatat taatgacccg gcaataatag aaaatctacg ctcaataatc
     7141 atctcaggtt cacagcgcga taaaattgaa gcgcttcgtg tattgagcga gataggtcca
     7201 acggatgacc tggaaatatc aaaaatttta tatttagcct tcaaagataa aaagatgatg
     7261 gtttccattg aagctatacg cacaatggga tatctaaaag ataagtatgc catacctcat
     7321 ctggttgaag cactggacag caaacattac cagataaggg ttgaagcaac cagagcattg
     7381 ggtatgatag caactgagca ggcaattgac ccgttaattg gatgtcttgt tgacaatcat
     7441 gctgaagtgc gcaaagctgc tcatgatgct ctgaagaaaa ttggaaccga caaggcgctc
     7501 aaggctatta ccgatgcacc atttatgctg ctggtaaaac acatgaccga aggcggctcg
     7561 caacgcgagg aaacggtaaa gcagattggt cagtacagga taaaagaagc cgcacctctt
     7621 cttattaaag cctgcaatga tgaatttaaa tcagttcgtg cggctgctgc ccgttctatt
     7681 ggcatattac gcgaaaaaag tgctgttaat gcgctcacca aactattaaa cgatccctac
     7741 tacgatgtgc gccttgaggc tgtcagagct ttagaaaaga tatttgatcc tcaggcgctg
     7801 aaagccattg aaaaagcaat gagcgattcc aataaaaatg tccgtgatga agcgcgcgta
     7861 gcgtattatt cccttaaatc ccggctggaa aaagtttacg aaacgtagat caggattgct
     7921 tcatgatact tggcttcaat attggcaata ccaataccgt tatgggcatt tacgatgagg
     7981 ataatccaga gccagtatac gccactacct accctacccg acaaacaata caatccgatg
     8041 aactatcgca cattatatat aatactttga aacaatggga taaacgcctt gcgtccagca
     8101 ttaccggtgt tatctattca agcgtggtcc cacaatccaa cacaacatac aataaaacca
     8161 ttgaagaact ttttaaggtg acagttcata gcataacaca cctttcaaaa atgccaatca
     8221 ggatagaata tgacaatcct gcacagttag gcgtggatag gatagtaaac gccgttgctg
     8281 cttttaccga ttatgcatgc gattgcatca tagtggacat tggtactgcc attaccttct
     8341 gccagcttac acaaggggta ttacggggag gaataatagc accaggcgta aaggtagcaa
     8401 tgaagggttt agcacagtct accgctcaat tatttgaagt accttttgaa aaaccaccct
     8461 atattattgc tactaatacc agcgatgccc tcatttcagg ctttttttac ggctggatag
     8521 cgcttataga gggaattata ggcaaaattc agtcaacgat gggcacctgc atggtcattg
     8581 taaccggtgg gatggctgaa atactctatc cgcacctttc aatctcagct attgttgacc
     8641 attcattaac catgcgtggt attttttatt tgtataacag taaccgctaa gcgtgtaaac
     8701 ataaataaaa cagtcctact atattaatat tcacaaagtt atccacacta tccacattga
     8761 cagcatctca tgacattaat ttactaaatt aattggctta tcgctgctac acgattacta
     8821 tgtcacatta ctacactgaa atatcagtaa attttttgat tgagccttat ataaatgctg
     8881 gaaatacgag gagtttcatt gcaatatatt tttataaata ttttttgttt ttttttaaaa
     8941 atttttttat gactattcta ttgcaaatta acataaatta atttctgtta atttttcaat
     9001 tttgtggata gcgtgacaga taaacattgt attgccataa ttattgtttt gttgtattta
     9061 cacccgctgc tgctcaagaa aaataactca acgcatatat aggtttactg ccatccaata
     9121 cgcaaaatta tgaaaaaatt tataattcta ctataatcaa ataatttttg attgtcacaa
     9181 ttatttatta tgtcctatca tgtacactga gtgtgcgcta taccattacc ctttacctct
     9241 tttgaaggtg agtggcgata attaggttaa tcattctatt gttttaacaa ggagattgct
     9301 gtacaatgaa taattacgag cgttatacac gcatattagc ccgttcggtt gaccatattt
     9361 ttaaacattt tctttatgac aatgatatca aagaggtctt taccaatcaa tcaacaccgg
     9421 atgaccccag ggtaaccatc cagcttgacg gaacgatgaa aggtgagctc atcataaatt
     9481 ttcccgaaca aactttacat cagttgacta aaaatttttt tatcaatgca aaaggcaata
     9541 taaagaaata ctatcccgat gtagccggtg aggttgcaaa cctcatagca ggaacattta
     9601 taaatcagct tcagtttata aagcataatc tcaaaccatc accacctgaa tacaacgaag
     9661 aacataccac tttaaaaaca ctctatgaaa atataaatct ttcatttgaa tcacggtttg
     9721 gcggttttga ccttgacttt tactataaag atctattgca ccaataatgg ctgtcataga
     9781 ttatatcatc atcgttatat attttctgtt cagtattggc attgcttttc ttttttcccg
     9841 acgggcgcgc actaacacca ggcaattctt cctttccggt caaagccttc catggtggat
     9901 aaccggtaca gccatggtgg caacaacatt tgcagccgat accccccttg cggtaaccga
     9961 gcttgtccac agcggaggta ttgcaggtaa ctggctatgg tggaatatgg ttgccgctaa
    10021 tgttgttacc gtgtttttct ttgcaaaatt atggcgacgc tctggtgttt taaccgatat
    10081 tgagttcatt gagctgcgct acagtggcaa agaagcatca tttttacgag gattcaaggc
    10141 attatatctg ggactttttg ccaactgtat cattatgggc tgggtaaacg ttgctctggc
    10201 acagatttta gcacatcttt ttaacataaa ccattcatac gtactgttca ttgtcattgc
    10261 aacaatgctc tttgtgggac tctattctgc aataggcggc ttatgggctg ttgctgttac
    10321 ggattttgtt cagttcatta ttgctatggg tggatgtatt gtgcttgcag tggttgtcat
    10381 tgatcatccg catatacagg gcatcgatgg tttaattcag tccatacccc aaaatgctct
    10441 tttatttttc ccctctggcg atagttcctc cctgccgctc tctgcctttt tgttgtttat
    10501 tggtgttcag tggtgggcat cgtggtatcc ggggtcagag ccaggcggtg gcggctatgt
    10561 tgcacagcgt atgatgtctt ctaaggatga aacacactca ttaaaagcaa cactgtggtt
    10621 taccatagca cattttgcct tacgaccatg gccatggata atcgttgccc ttgcaaccat
    10681 ggtgctgtat cccactatcc ccgctgacca atccaaagcc acctatgttt ttgcaatacg
    10741 cgattatctg cctgccggct taaagggtat gcttgttgca gcattcttag cggcatacat
    10801 gtcaaccatt gccacccatt taaactgggg aagctcgtac cttgtgaatg atctgtaccg
    10861 acgatttata gtaacgggaa aaagtgaaaa gcactatgtt tacagcgcac aggtgtgcac
    10921 cacattgctg gcagttattg caagcctgtt aatctttgtt atcgattcaa tatccggagc
    10981 atggacattc attattgaat gcggtgcagg catagggccg gtgctcatgt tgcgctggta
    11041 ctggtggcgt atcaatgcat ggtcggagat tatagccatg atagcaccca tccccgcggt
    11101 tattatatcg cggctggtac tgcatattgc cttaccctat aatctgctca tcatcgttcc
    11161 atggaccaca atctgctggg tgatggcaac ccttgtaacc caacctgtca aacatgaaac
    11221 gctgacagca ttttatcaca aggtacagcc tgaatttggg tttgagccca tacgcaaagc
    11281 gctccattat cctccgggta actatcaaac atgtatgata cttttattcc agaccacact
    11341 tggcatcata gccacctact cgctgctctt tggcataggg gcagtgttgc tgcacaaaac
    11401 aggagcatac ctctatctca ttgcatttgt cgtcagcttt atgctggtca tctattcttt
    11461 tcgcatgaca aaaaaataat aacatcatta tgtgcgatag ttactgtata gtgtacctgc
    11521 tttgttaaaa agtaacaagg caccgtgtca atccaagctt atcgcctgtc aatctgaacc
    11581 aggtgaagaa tcccaccgtt ttgtgtgccg ctggcattct gagcggagtg aagaatcttg
    11641 tcgttttgtg aacgaatata ttcttctggg attcgcccct cagaatgacg aaaagggata
    11701 tcgttattat gattccatta catttatgga tagaatgtaa tcattgcaaa aatttttatt
    11761 tttgtaaagc aactatcaaa atattttttt ttactattcc tgagcgcgct atttttttat
    11821 tgatccttta tactttattt tgtataaatt ttaccataag tctggacgga attaaattta
    11881 accatgagca ttgttgaaaa attagcacaa gttattgccc atgatatgca ccttgatgaa
    11941 aaaactatca acgagctgca cgatctgttt accataagcg atgacgatac aaaggcaata
    12001 gaaaaaatac aggaaatcat aaaattgtca cctgatttct ggaaaaattt ctatagccgg
    12061 tgtttaaacg atactgcatt gcaaaaaatt aattctgatt cacaattttt taaaaagcac
    12121 aaacgtgcca ttatagacta ttttataaaa ttaccggaac aaaaaattga ttatgattat
    12181 ttcattagcc gctttgccat aggcatacag cttgaaaaag ctactgtaga gccaattctg
    12241 tttgcaaaca tcttgagcaa tttgtatata cagctcatac agtattgtga ggatataatt
    12301 catgatacat cactacttac agagataatg cagtcatact taaaaattgt ttttttagat
    12361 ttaacattga tactgcatgc ctattttttt atgaaggaaa aaaagctgtc cgatgcaaaa
    12421 aaggaactgg agcgcctggg aaggtcatat cgtttaatcc atgaaatcaa tgatcttatt
    12481 gttcgcacaa ccgatgaaaa caccctgtat gaaaatgttg ctaaaatact ggttgtcgtg
    12541 ggcaattttg accttgcatg gattggtatg gtcgataaac agctcaatgt agtaccggtt
    12601 gcatcgtatg gagaaaccca gtacctgaat gatatatata ttaacattga cccatcattg
    12661 cccgaaggta atggacctac gggaactgca atccgggaca aaaccataat caggattaac
    12721 taccccgatg aagatccgcg ctttagcccg tggaaggaac gagccgccaa atttgatttt
    12781 aaatccattg tagcaatacc catcatcgtt gatgacaata cccatgcaat tggcgccatt
    12841 acgttatatt cgcataatga acactttgtc acactgggtg aagcggcact tttgcaggag
    12901 gttgccaatg atatctcctt cgcgatagac cacatacgaa aaaataaaaa gattgaaaag
    12961 atcctttttt atgatgagct taccggtctg gggaattaca attacttttt atctgccctg
    13021 acctcacaga tacagttagc ttcatcacaa acgatgcaat tggccgttgt tactattgat
    13081 attgataact ttactgccat caataactca tttggcttca acacaggcga tgctatactg
    13141 cgtgaatatc gtaaccgact tcaaaatagt atagatgcaa atcttattgc acgcgatggc
    13201 gatgagttct tcatacttta tccttacatt gatgagatgg ggctgacatc ctttatcagc
    13261 gaccttaaac tgatagccac aaaaccatta attgctatga gccatgaact ggttatctcg
    13321 tactgcatcg gtattgcaac ctatccaaac gatggcaaaa ctgctgagga gcttcttaat
    13381 aaatcaagga ttgctctgac cgaggcaaaa aagaagggaa gcagcagtat atgctactat
    13441 tcagaggaac tttttcaaaa tagcattcaa aaaattgctc tggaaaagga attgcgcagt
    13501 gcactcaaaa acaatgaatt taccctttac tatcagccaa aaatagctgt agcgtcgcgt
    13561 aacatttcag ggctggaagc gctgttacga tggaatcacc cggaaaaagg cttgctgcca
    13621 ccatctgatt ttattgctat tctggaatca tctgatatga taatccagat agggaaatgg
    13681 gttgcagcag aggtggcaag gcaggtgcag attaatccaa tgtttcagga aaaaagtttt
    13741 atcatgtcat ttaacgtatc ccccaatcaa ttttatgatt cagcatttat atttgatata
    13801 atcaaaatag ttaaaagctc ggctattaat ccctgcaatt tggaaataga agtaactgaa
    13861 aatattttaa tgtcggatgc agagaatgtc attgatacct gcaataaatt acaacaattt
    13921 ggcattaaca tctcaataga tgattttgga acagggtatt cgtcactatc atatcttaaa
    13981 aagatgccgg tatctacttt aaagatagat gcatcgtttg tcaatggctt gccgcataac
    14041 aaagatgatg ttgaaattgt taaaaccatc atccttttag cccactcact gggtaaaaaa
    14101 accatcgctg aaggagttga aaccagggaa caattagatg ttcttgtaga tttaggggtt
    14161 gatgaggttc agggatatta tttttcaaaa ccaatgccca tccatgaatg tgttgattat
    14221 tgtaaaaatt ttaatcccga caattatttt taacaaaaac aatatccagt aatagtgcag
    14281 tatccatcct ggtactttgc tatcaggcac atcatagtaa taaaataatt ccttgacttt
    14341 cattgccatg catacccttt ttatatagaa gtaatgatac actcaagctt tcaaggaaaa
    14401 acccatgatt cccagtatag gtaaatgcca tgcaggtagc agtagcagca atggcaaaac
    14461 tttttcatca ctatgaagaa taacaccatg aaggcaaaag caatagccct gtattctggt
    14521 ggactggata gcctgcttgc tgcagcgata gttgctcgtc agggagttga ggttattgga
    14581 ctgcactgca ttttgccatc agttgacccc caaaaacctc ccgatagcct tgcggctgca
    14641 caactggcaa agcagattga tcttccactg gaatactatc gctgcgacga acagtatatg
    14701 caggttattg cacatccagt acatggctat ggtaaacgtg caaatccatg tattgactgt
    14761 aaaattttct ttttacaaaa agcaaaggag tatatgcaaa ggcataatgc ttcatttgtc
    14821 attaccggtg atgtggttgg tcaaaggccc atgtcacagt acaaagcaat gttgcgacat
    14881 atagaaaaag aaagcgggct tgaagggata ctgctacgcc cgttatcggc aaagctgcta
    14941 ccaccaaccg tccctgaaca aaaaggtatt gtcaacagag atatgctcta tgctatatca
    15001 ggtcgttcac gtcagcagca gatgcaatta gcacaggatt caggaataca ggattatcaa
    15061 tcgcctgctg gaggttgctt tttaaccatg ccccagattg catcacgggt taaggacctg
    15121 tttgcccatc ctccttacag cacccttgat ctttaccttg tcaacattgg gaggcactat
    15181 cggttacatg ataaagcaag tatcatagta gcccgcaatg aagaagaatg cataatactt
    15241 gacaggtata aacagcatgc tgatttttat gttgaaccag attttaaagg tcctgtcatg
    15301 tatggcaggg gaaccattac gcaaaacgac tatctactgc ttacacagat tatggctcgc
    15361 tatggccgcc caacaatgac agaaaatagc attgcactat taatacaggg acagcgtata
    15421 agtaatctta tggttgataa acccataggt gatgctctgt taaatcaaat gcggatataa
    15481 ttatggcacc ggagtaatac aatgaaaaaa actgtatcat ttttagtttc aggacgtggt
    15541 tcaaactttc aagctgttgc aaaagccatt ataaagggca ccatacctgc aaaaacgggg
    15601 gttgttattt ctgataccga aggggtcaga gcccttgaca ttgcacagga gctatctatc
    15661 ccggcttttt ttattaaccc cgggcagtac aaaagccgcc aggaacatga aaaagctatg
    15721 gtacaattgt tgcatgaata cagtaccgat gtcgtcgtta cagcaggata catgcgcctt
    15781 ttaacaccgt attttgtaca tgaatttaaa aatagcatta tcaatataca tcctgctctt
    15841 ctgccagcat ttcccggtac acatgcacaa aggcaggcgc tggaatatgg tgtaaagatt
    15901 tctggatgca caacgcactt tattgatgag ggtaccgatt caggtccaat tattttgcaa
    15961 aaagctgtcc ctgtttatga cgatgatact gaggatacct tatcggcgcg catcctcaag
    16021 gaagagcatc aactattgat tgaatcagta cggcttttct gcaaggataa gatttttatt
    16081 aaaggcagaa aagtttatat tgtgcgatag ttactcctga ataagctttc cgggttaaaa
    16141 aatacttgca aaatttacac taatcagtat ttttttatta cgatattatt ttatgtattc
    16201 cgggataatg catagttgct ctgtagccgc acaaatagtg ttactatgca acaccactgc
    16261 catggtgcat agtggcgcct gtgttgggcg acacatcatc ttgaaccaac gacggaggtt
    16321 ctcatcgtct ggtgaatgac cgtcatcctg agcaaggcaa agaatctcgt tgttttgtga
    16381 accaattgat tcttcggggc ttctcccctc agaatgacaa tgtgcggagt gtcatcctga
    16441 accatgttct gaactggttt caggatctaa cgttgcacat ctggattccg gaacaagtcc
    16501 agaatgacgg tagcggcaca atagattctg tgttaagcac ggaatgacag gaatgcataa
    16561 tttaattttt tgaggaggaa tcacatgggg ttaattgcaa tacttttatt atgtgtgttt
    16621 tatctaggat taatcataat tatctatgaa tactatacgt tattttcatc aaaagatgaa
    16681 tatacaaaac aggaactaaa aggtgttatc actggcgctc ctgaccactg gatgccatta
    16741 ctgtcacaat tcagattatt ccctgtttat gcactggcat cacttatcgt tgggattctc
    16801 atccctctgt tatcatataa ctggttcttt caatcatcga tattctctat acttttattt
    16861 atcattttac cacaggtacg tcgttcttat gaaccagtaa aagttaccat aaccgactcc
    16921 atctgggata tcatagcagc tgcactatca gaataccacg agataatgat agtattcttc
    16981 agcacaggca ctgtagcatc attaacatat tgctggatat caaatcgtga attgccattt
    17041 ctatggttct ttgtcaatgc aatcattata tgtgttatca tattttttac attggtatcc
    17101 atagataaag aaaataataa ataaaatcat taattttttt gcactttttg tttaatacac
    17161 agtactacag tttattattt tctgaacaat gttagttcat gctatcacca aaaattttat
    17221 tttttgtgtt gacggtttca ttaaattttt gtattattta ctgagtgtac ggcttgtgcc
    17281 atgtacacag tgttttaatt aataatatat catggggtta accccctaaa gagaaggagg
    17341 agtcttatat gaaaagattt ttgttagttg cattaatagc tgttgttgca attagtttca
    17401 cagcatgcgg aagttccaaa atgggtgggc aggtaaaagg ggatagctat atttctgaag
    17461 gatgggtaac acccgatatt ttccgagtaa ctgcaaccgg tgctccaaaa ccaggactta
    17521 aagacagggt tatgagacga ggaacagcaa aagaggctgc tattgcaatg gctgaaaagc
    17581 gtattgtaga aaaatttaaa ggtgcccgat tagaaggtgc tgctggagct gctgactatg
    17641 catcgacagg tgtagcagtt gcaaaagaat ttgaaggttt agtaaaaggt ggaaccatag
    17701 ttaaagaaac gtacgatgaa gaggatcact gcgaaatcgt atatcagatc gagaaaaagg
    17761 gattaaagaa agaggttgaa ggcggcgctt attaataata attagcttcc atgtaatgac
    17821 taaaaagcag gcatgtcttc ctgctttttt attttattca ttgattcagg ctgtcgaatt
    17881 aattgacaca aaaaggataa tatgacaata gtattccaca atcaagcaaa aaatgcaggg
    17941 agaaagcgat gagacaattc agggaaccgc gtcatagacc actcgacgat agggaagttt
    18001 ttggttcgtt tcaagaagga gcttatagag atgtttgtgc aaacggttca gcttgccatt
    18061 gagaaggggc tggtgaattt tgaagtattg tcaattgatt cggtcaagat acgggcaaat
    18121 gcgtcaagca agaaggacaa gagcattgat ggtttgaatc ggctggaaga caccattaag
    18181 gagaaactaa cgagattcat ggatgatgta gaccgagatg agcgcaggga tgaagaaatt
    18241 gaggcattgc gtcgtcagga ggcacgggta gtcgaggcaa aagaggaact gcggaagcga
    18301 attgagaaga agaaggaggg caagtcaggt aaggagctgg agaagattga gaaaaatgag
    18361 aagatcaatc taactgattt tgatagtcat acgatgcagc aggcgaatgg tgaaattaac
    18421 cccgcctatt cgatgacgac tgcggttgac gcacatcatg atattattac ccattttcaa
    18481 atcaatgagg aagacaatga tggtaaggcg cttgttccgg ttattgaagg gagcagggaa
    18541 aagtgtgggc aatctcatga aatcgttgct gcggatgctg gttttgcatc tcttgataat
    18601 tacgaaaaac ttgctgcgat gaatcaggaa gcattgatac ctgacaggcg gtatgatgtt
    18661 gacaggcgtg gtgttcagaa gaaaggagcg tttgatacat atcatttcac ctattgtcct
    18721 gaaacaaact ggtatcaatg tccgcatggt ataccacttt tttttcagaa tgaatatagt
    18781 gctggtggga ggaatttttt tcgatatgct aaccgggctg catgtttatc ctgttgtgat
    18841 cgaacgaggt gcacgaagag tgagtatcgg acaatcaccc gagataaaaa ggaagggtat
    18901 cgtgaagcga tgaggcattg tttatcgaag agcgaaaaca aggagaaata caagcgacgg
    18961 tcacactgtt ctgaatcacc gaatgggaac ataaagcaca atttgaaata tcgcatatac
    19021 atgcgacggg gacgagataa ggttgcgatg gaagggtcat tgctctgtat gatgcacaat
    19081 atactgaaaa tgggtaattt cagtatgcag atgctataga ttagcccacc atgggtagtg
    19141 gtgagctgtt tgagcgatgc tatctggtat aaagtccatt tgttataaag cagtaagggg
    19201 aagtcaatag tttaaaagtt aaaattcgac agcctgatta atagaattaa tgagaccttc
    19261 atctccattc aaccaaagct tgccgtctgg gtatgcgata gtattttgca atgggatatc
    19321 cggtcagcat tgttccatat attccattat catctgggat actcaacgct ttccttaatg
    19381 gaggatatgc catagcacac catgtaaaat atcctgccca gcagcacccc aatccaaatg
    19441 cttgcgctat taattccaaa tgtgcaagtg ctatagtaca tgaagttggg gtattgggat
    19501 tgtttttatt accatgtgct actataacca ctggtgcatt gtgcatgaca acatcaacac
    19561 ctgaagccca tgcttttgtt atcatgtcaa aatgcatgct tttacctata tccggagatg
    19621 aactgatcat taattgcatc caatcaataa catgttctgt taattttttt atttcttctt
    19681 tctgtgtata cacaataaat tcaacagttc gcgaattatg acctgttggt gcatatgctg
    19741 ttatatctaa caatttttca agcttttctt tctctactgt tttattttta aatctgcgta
    19801 ttgagcgtct tgatttaaaa taatattcga tgatctcaat acccgggtta aaattttgtg
    19861 aaatactctg acattcttca accttcattg atgttaaact taacgcccca tatggacaaa
    19921 cagcaataca gtgcccacaa tcaagacaca tgttctctgc attgtgtatt gtttctggta
    19981 tacctgtatc actgttcaat ataatgatct tcatgggaca ttccagcaca cacaatttat
    20041 cttttttaca cttttctgta tcaattatta ctgctgacat gctttattat cctttcaata
    20101 caatattctt atcatgttaa tttattactg aaataaaatt atggatatct ctcaaatcag
    20161 gttttgcatt attcaccata tgataaaaca ttttttcaat acttgtttcc ccacgattac
    20221 gtccataatc tcaatatcag aggttatcct gcacataaca aacttcctga atattgaatt
    20281 cacctttcaa aacagttatc gctgttccac caataaacac cctgttatcc ttcacttcaa
    20341 tcttcagcat cccaccacgt tgtgaagcct gatatgcaac aaagtgattt ttatgcaatt
    20401 tcttttgcca gtaaggtcct aaacaacaat gtgctgaacc agtcactgga tcctcattta
    20461 ctccgattcc aggtgcaaaa aatcgggaca caaaatcaaa ttcgtttttt gaagattgcg
    20521 cagtaatgat aacaccgcga ctatcaaggt tttttaatgt aataaaatca ggacttacca
    20581 tgataacttc cttatctgat tctacttcta ttaggtaatc catcctattt ttgcctgtaa
    20641 aaacaggttt tactcctaat gcatcaatga tccattgctg gattgaacat tcacttgatt
    20701 cctcttttgg gaaatccatt ataattgtat caccctgact ctttaccaaa agcaaaccgc
    20761 ttctggtata aaatctaatc atttgatctg gattaataat ctgctgttga taaagaatgt
    20821 gagctgtagc caatgttgcg tgcccgcata gatccatttc ctgtaatggc gcaaaccacc
    20881 gtaatgaaaa accatcatcc tgtttcatga caaaggctgt ttcaggacaa ttcaattcat
    20941 acgctatagc ctgcattagt tgatcagata ttgtagaaga caatacacac acagctgctg
    21001 gattaccttt gaatggctct tgtgtaaatg catcaacaat atagagtggt atcacttttc
    21061 ttcttcaacc ttttcaatgt tatggaataa ataatgcata aaacagcata tcatttttct
    21121 ttaataattt gtctcatcta tcatgtcaat aagttcacca aaaaataatt gagacctctg
    21181 aacaaatctt taaaaaaatg cttgcaatag aaaaataa
//
LOCUS       DAOOJH010000035        21074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2511_length_21074_cov_10.562681, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000035 DAOOJH010000000
VERSION     DAOOJH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 21074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21074
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_21074_cov_10.562681"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PLJ75_06420"
     CDS             <1..366
                     /locus_tag="PLJ75_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD04888.1"
                     /translation="TVSSDVMQRALLAFHFIRESTLLMLDNPTQNLSLSSYTILHNAI
                     IKYTSSGKNLCIIASHDLNFLTQTTDKLYIMDTGTIASWHLPADINETIIKKYLGVDV
                     FITRNIYNGKPNVHLYFES"
     gene            complement(379..726)
                     /locus_tag="PLJ75_06425"
     CDS             complement(379..726)
                     /locus_tag="PLJ75_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04889.1"
                     /translation="MAAEINYSIEEREGAKIIHLSGVLCSATEREFINLVESLIATNN
                     VIINFSATSMVTSSGLRALIDVSHLARQKKRRVMILGASENLHGLINVTESYDLLLIN
                     SVEEGLRKLEYYL"
     gene            complement(731..1819)
                     /gene="aroB"
                     /locus_tag="PLJ75_06430"
     CDS             complement(731..1819)
                     /gene="aroB"
                     /locus_tag="PLJ75_06430"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPD04890.1"
                     /translation="MDSKINSETIVAHYSSKQSTITIGHGIFNFLVRLLPYSKWDVIG
                     AIISDRVYLLHRQTIDDSLQEINACIKGYDDTEYNKSYAYAESYFNYLLHNQFSRKSL
                     LIGIGGGVTTDFTGYLASSYMRGIDIVYVPTTLLAMVDASIGGKVAVNIEAGKNIIGH
                     FHQPDYIIVDMGFLTTLPVEEWKCGMSEAVKHAIIGDSSSLNLFLRREVSIGNVNESL
                     YHCVKNSVHFKANIVEQDEFEKNIRSILNYGHTVGHAIESVFHYTLSHGEAVAVGMLV
                     ESYISMKMGMPQEDFEMIKELLLYYKLLPDISFSTSDIIDHMQYDKKNIKGTILFVLL
                     EKVGKPVIGQEVPGTLITEALNYYLTMV"
     gene            complement(1804..2268)
                     /locus_tag="PLJ75_06435"
     CDS             complement(1804..2268)
                     /locus_tag="PLJ75_06435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD04891.1"
                     /translation="MRFKLSLSHKKIRIRVAGLVVKDDTILMIAHKKGNDIYWLLPGG
                     GVEYRESLDNALKREFKEELGLDVEVGDIAMIADSIEPNGRRHIINVIFWCNLVSESI
                     QLGNEKILYGYSFKPIQEIDTIVIYPPLNTCIIQLLKNKNEKIYKGSLWIPK"
     gene            complement(2265..>3013)
                     /gene="rnc"
                     /locus_tag="PLJ75_06440"
     CDS             complement(2265..>3013)
                     /gene="rnc"
                     /locus_tag="PLJ75_06440"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPD04892.1"
                     /translation="TMNDDKTKGSEKKRQNRKLQQLEKRLQITFNNKSLLRRALTHRS
                     FVNESPVPVKDNERLEYLGDSVLALVVNEYLFKSFENYPEGDLAKIKSAVVSEVMLAN
                     VAINLNLGDFIFMGRGEELTGGRQRQSILANTLEAIIGAVYIDSGLTKSKKLVLRLLK
                     KDIQRIDKLSYLRDPKTTLQEIVQKKYKNRPIYTVIQESGPDHSKIYTVKLVINDEDI
                     TMGSGSSKRKAESDAAKKALELIYSDRIHI"
     assembly_gap    3014..3113
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3297..3491)
                     /gene="rpmF"
                     /locus_tag="PLJ75_06445"
     CDS             complement(3297..3491)
                     /gene="rpmF"
                     /locus_tag="PLJ75_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPD04893.1"
                     /translation="MAVPKRRKSKAKSRMRRSHDAIGIPNLRPCPTCGAYILPHRVCP
                     ECGHYKGKLVIEPVVKVTKG"
     gene            complement(3581..4282)
                     /locus_tag="PLJ75_06450"
     CDS             complement(3581..4282)
                     /locus_tag="PLJ75_06450"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04894.1"
                     /translation="MIDRQVSVKRNIVEQTLMNVRDYIRAHQSIVVKISIVSGIVLFL
                     VIAGIVFCDYKINKETADFEAVLEEYRNTYTLDEIQKQEKFKRTVNKLGSIVDSSYFG
                     YVHKNGYYIIASLYFNEKDYSNAVKYYEKAYRHCSPLFAPLALQQAGIACEYINNIDE
                     ALRYYLLCESKYNTSYNNDQILFNIGRMYSLKGEYFKAREYFTKVTVEFPQSVFAKRA
                     MQYGMFLGAIESGQK"
     gene            complement(4289..5956)
                     /locus_tag="PLJ75_06455"
     CDS             complement(4289..5956)
                     /locus_tag="PLJ75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPD04895.1"
                     /translation="MNLERSDSQLYMNHVSSLSNDSIQTKTIYNGEIVTVDNDFAYVS
                     IGTKTDGRVSLEEFEIAPKVGDIVEVMLVNRRLLDGMYVFSIKAAKREKSWQQFLKAT
                     EGSSIIQGIVTQKSKSGYIVDCDGFHAFLPLTLAADVKGKSQNGHSSVYFKVKNVDKR
                     RRSVVLSRKDFLDEEQQKIWDNLEKNYSIGDVIRGKVTKFVDFGAFIDIGGIDALLHR
                     NDISWRKVFKKKKILRIGEERDFVLLSFNRAEGKIALGLKQLISDPWLSVPEKFKVGE
                     ITEGKVVTLTNFGMFIEFEAGVEGFCASTDISWTKRMVNPKEQYKKGDRVNVFILDVD
                     LENRKLLVGIKQLYPNPWDTIQERFPLKSVHTRPIKKILNFGMFVELEPDVDGLIHIS
                     DISWDENFKDIHSQYKEGQEVQCTILEINKEEQKIACGIKQLTKSPWEIIADKYKPRT
                     PVSGTITGMVPFGIFVKIDGDSVEGLVHISEVSRKKIDSVEDIFKIGDKVNAVVLGVD
                     VAKKRLSLSIKHYEIMSEKEEIKKILNTPTAGTVTLGELLKNKLDKE"
     gene            complement(6133..6816)
                     /gene="cmk"
                     /locus_tag="PLJ75_06460"
     CDS             complement(6133..6816)
                     /gene="cmk"
                     /locus_tag="PLJ75_06460"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPD04896.1"
                     /translation="MNKNIVIAIDGPAGSGKSSVAREVAKRMGLQYIDSGALYRAITL
                     YVLEKSGESFTRDDVCRHLNAIHLEQVFNEDGSCTTICNGIDVSEKIRSETIASHIGK
                     ISDIIEVRNFVNSLMREWAKGNSIIVDGRDIGTVVFPDALIKIYLDASVDERAKRRAL
                     EYSLKGKEVDLDELKTQIRRRDEQDMKRKFGALKKSDDAIYLDTSNMTQGEVVHSIYS
                     LINTKIQYC"
     gene            complement(6809..7684)
                     /locus_tag="PLJ75_06465"
     CDS             complement(6809..7684)
                     /locus_tag="PLJ75_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPD04897.1"
                     /translation="MFNTVAIIGLGLIGGSIARMLKKKNHEIVISAYGRNPELLQSAK
                     EEGIIDNIGSINKIDCSGVDLFIVATPVLSSIDLIQSLLSSDDLKNEALIIDAGSVKQ
                     SIIDAIIAHDKACQFIGCHPMAGSEKSGYQYSSHSLLGNAWVIITPHDKNKESDIKKI
                     EAFWKMLECNTIIIDAQLHDTGVAYSSHLPHIASTALVDSFDSITNNTYSTIEPFIGK
                     GFRDMTRLAGGKPDMWADIVLLNHGNIVNALDSYINSLLHIKSIMQQAAQTNDRTLLY
                     RYFATIRQKWEKYNE"
     gene            complement(7684..>8187)
                     /locus_tag="PLJ75_06470"
     CDS             complement(7684..>8187)
                     /locus_tag="PLJ75_06470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935231.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPD04898.1"
                     /translation="GLIAECADVLQVGTRNMQNFNLLKELGKIDRPILLKRGMSATIN
                     EFLMSAEYILSQGNKQVILCPRGIRTFEDVTRNTLDAGAVPVLKDFSHLPVICDPSHA
                     MGLKDYVVAAALAYVAAGADGIMVEAHPNPEEAMSDGQQTINYEQLVDLKKKLASVAL
                     ALGRGMY"
     assembly_gap    8188..8287
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8288..8472)
                     /locus_tag="PLJ75_06475"
     CDS             complement(<8288..8472)
                     /locus_tag="PLJ75_06475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037053.2"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPD04899.1"
                     /translation="MIIVLKPNITQDEIDHIVGKITSLNLQPHVSKGQYRTIITVIGD
                     EDIIREQPLEAISGVESV"
     gene            complement(8502..9548)
                     /gene="pheA"
                     /locus_tag="PLJ75_06480"
     CDS             complement(8502..9548)
                     /gene="pheA"
                     /locus_tag="PLJ75_06480"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPD04900.1"
                     /translation="MENNNFENTVIETIAQYVDTYIKELKDIVQDGEQGIFAVKAKMI
                     SNLKQHARPPITIELVEKIATEIFSLAVSVVKPIKVAYLGPIGTFTHIALLEIFGDAI
                     EAIAHRTISDVFDEVETSRATFGVVPIENTTEGAVTYTMDEFLETDLNIVSEKYLRIT
                     YSLLSVCDNIDKIKKVYSHPQSLAQCKNWLKANLPNVEIIQLNSTAKAAETASWDKYS
                     AAIASTISAKIYNLNIIADGIEDSRQNFTRFLVLGKHANPPSNNDKTSIVCAVKDQPG
                     ALYKLLKPFSDHGINMTMIESRPDKRKMWAYNFFIDFMGHRTDPVVQEALENMKQETI
                     FLKILGSYQAERHE"
     gene            complement(9550..10062)
                     /gene="scpB"
                     /locus_tag="PLJ75_06485"
     CDS             complement(9550..10062)
                     /gene="scpB"
                     /locus_tag="PLJ75_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPD04901.1"
                     /translation="MIEAILFISSEPVPLNYFTKQMGIDATQAKIIIDSLIDEYEDRD
                     RGIKLVEISGGYQFVTNPKFAATIRQAMGYKKKETLSKGMLETLAIIAYKQPIMLAEI
                     EELRGVSSRMMLAALMRKNLIKPVGRKDIPGRPLLYGTTDEFLKYFGLNKLSDLPKLS
                     EIKEYNFEEE"
     gene            complement(10205..10954)
                     /locus_tag="PLJ75_06490"
     CDS             complement(10205..10954)
                     /locus_tag="PLJ75_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPD04902.1"
                     /translation="MVSSQEIEAQCILHINNFEGPLDLLWELIKKSKIEITQVSIAEI
                     TDQYCNYLSLMEKINIPIAMDFIVMASELIYYKSKALLPGSDIGDEFFVPPLPPELIQ
                     KLLEFKKYQIASKNLISLHAMQSDVFARQPNPVNNDEVVYLEVNLFDLLNAFVDVLES
                     EKEVESQSIVFDEILVSDRIILLISMLQDTEQIEFNELFPQKPARMEVVVTLLAVLEM
                     IRIGAIAVLQNAQFGNMYIFKKSLEKAELIY"
     gene            complement(11097..11456)
                     /locus_tag="PLJ75_06495"
     CDS             complement(11097..11456)
                     /locus_tag="PLJ75_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04903.1"
                     /translation="MAKILIVDDAKFMRTLVKDALVPRGHEIIGEAENGVEAVQLYKK
                     LRPDLVTMDITMREKDGIEAAEEILLFDPNARIIMVTALGQENLLTRAIKIGVKDFVV
                     KPFPPERLQKAADKALA"
     gene            complement(11640..12686)
                     /locus_tag="PLJ75_06500"
     CDS             complement(11640..12686)
                     /locus_tag="PLJ75_06500"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPD04904.1"
                     /translation="MSQKIKVMVVDDSALVRKVITDILESDKDIEVIGTANNGKSAVF
                     KNLTLNPDVITMDIEMPIMDGLEALQEIIKTNPKPVIMMSVLTQYGAEATFRALEFGA
                     IDFIPKPSNLLAMSVDDIAELLISKVKAVARIPLKISHSKDIVNRQSTKLTVDSNQKR
                     VKKIVGIGTSTGGPSALQRVLQKLPRNFPSPILVVQHMPPGFTKAFAERLDSLCHVSV
                     KEAEDSEEAMDGCVYIAPGDSHMVMEKMNNKRIIRLSQSDKVSGHRPSADVLFQSMVK
                     VGMENNIGVIMTGMGRDGAMGIKEIHNNGGYTIAQNESTSVVYGMNRVAVEMGAIDAV
                     VDLDSIPDEIVARI"
     gene            complement(12692..15748)
                     /locus_tag="PLJ75_06505"
     CDS             complement(12692..15748)
                     /locus_tag="PLJ75_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD04905.1"
                     /translation="MGFSLGEYQDIFLEEADEQLQELNANLLQLEKNPSDEDIINNIF
                     RAAHSLKSSAAFVGLTDLSDLAHKMENLLQGIRDKVMEITPSIVDVLFQCFDVINEVI
                     HEVSAGNQPTRDFTGLIKKIEGVSTHFENPAEKKEQPVTRKIQLPASIVKDIRDGINN
                     GLSCCEISITLDTDAAMKSLKVQLVLANLEKIGDVLVTIPAIEEVALPGFNNCVQIIL
                     LTNASVDEIRKACEVDQVIRIDLRKIELIKKDNKVALKYHPQETLFEEKQEAAPKQPD
                     FDEDMVPDEEEKDEIIIRSKEDKKTVQLRTVKVSVDKLDQLLNNVGELVIANSGFYKL
                     YEEMRRFSVEKTIINEFKNRMEQMSRIAKDLQSGIMKTRMVPIGQVFTRFNRLVRDLA
                     KEFDKKVIMEIKGEETELDKKVIDVIGEPLMHLIRNAVDHGIESVEERRQLKKPDVAT
                     ITLNAYQGGNQIFVEVSDDGRGLDYKKIKSKAIEMNLATPEIVANMDKDELASFIFMP
                     GFSTSEKVTDISGRGVGMNVVQETVNELNGSVSIESEPGMGTRFILSFPLTLAIIPAI
                     MVRVQRELFAIPLSDVIETIKILPDEITTIEGHEVINLRGEILSLIRLNNFVGINSIL
                     KDNERMPVVVVGYGNRKIGIVVDLLEGKQEIVIKSLEQNYKTVPGLAGASILGDGSIC
                     LILDISSMINKVIMQQDMLSHKEKQRVLDTRQASVEVVKHEATKDNAEKPEHDDHYKK
                     DIRESVTATEMPIKEDIIFKKQLNGEIVEIEETRVGESEKSTNNNIIERQVHNALEEF
                     KSELKEKIKTTLGEENKIYNKLGISDDDLAKVQVLANVGITDAAEKLSKILGRRVDLS
                     IPDVSVLAIETIPQFFGDIDSVYLGVYMPIVGQINGTILFSLHQESGYELVDMLLGLP
                     QGTTTSLSEDGESVLKEVTNIVGSAVVNAFADKIEMAIKPTVPSVVHDYMQSILDSIL
                     AFHTAENEYALVMDTEFFHQDDRVIGKLMIMPTTSSLSTIVERLRN"
     gene            complement(15785..16279)
                     /locus_tag="PLJ75_06510"
     CDS             complement(15785..16279)
                     /locus_tag="PLJ75_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD04906.1"
                     /translation="MNNELISIAAKTAESVIDTNDSLDIIQLVSFKLDKIEYGIDILS
                     VHEILRIPEITRLPNAPEYIKGVINLRGNVIPVVDIRMRFGMPSVPVTELSRIIVVEI
                     GEKLVGLMVDNVYQVIRLSRSRIDEPHELIEGISTEFINGIGRLQDRLIVILRLDNIL
                     FGEE"
     gene            complement(16634..>17152)
                     /locus_tag="PLJ75_06515"
     CDS             complement(16634..>17152)
                     /locus_tag="PLJ75_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04907.1"
                     /translation="LFNQMVCIVTNDGYLYAIDISLGVSIYRKHIGTVIENGLIAQYP
                     EIYFVTPDSIQKLHVLKDELVWSFNDSSIIDAMAYDNGIVYATTNGLLCKLSASGDSE
                     WKISPGKSIHSLKSIRNEITCIADTVFYLIASNGEILWSYTLPSPDATIVAVTPKMVF
                     ICIENRLLTLKI"
     assembly_gap    17153..17252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17253..18242)
                     /locus_tag="PLJ75_06520"
     CDS             complement(<17253..18242)
                     /locus_tag="PLJ75_06520"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPD04908.1"
                     /translation="MNRQIDDNELLHEIAPYAGKASDVLLKIIRQWQSSLPALEDTLT
                     SNDIIEKAKTGSHGIQIKKLVAMFATGIAAIVIAGVMLFTHYFSGTMEKAVCTAVIGD
                     VHVIRGNQSVPLMNGMGIQQRDRIICGKGSMAVIEIGSLKLRIDQNSEFAIDTVQSKG
                     KLLLDTSMTRGVIYATIAKLKKGDSVFVKTKTAVASIRGTTFMIKSNNNITKLIVLEG
                     KVMIAPRLDIIPQESTPGVVVDVGQACVVDAGTIIAIKKAVSMNTTELDTIIQQSLPV
                     TIADKKIIQIIETLTRDINQAIPVNENELTLNALYNIKAVVPDNNDVLVTTSDG"
     gene            complement(18298..18867)
                     /locus_tag="PLJ75_06525"
     CDS             complement(18298..18867)
                     /locus_tag="PLJ75_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD04909.1"
                     /translation="MDQSIKALSDEELVQSVMHGNDRAFEELYERYSERIFKLLYSYV
                     YNVDDAVDLMHDVFIRAYRHLNKFDVRRAFSAWLYTIAINCAKNYRCKVYKNDTMIER
                     EEQRIMNTDAMKTPEDHVIENELLQEFSKAVDALNNKFREVFLLRYGQQLQYSDIAHI
                     LDISERTAKWRMERALAFITDHLKKKGVI"
     gene            complement(18948..19289)
                     /locus_tag="PLJ75_06530"
     CDS             complement(18948..19289)
                     /locus_tag="PLJ75_06530"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPD04910.1"
                     /translation="MISSANNPNKVKVTILGQVYTIEGDASGDHIIKVADYVNQKMED
                     VANTISHANSLQIAILAALNIADELFQIKESEYMMSDEIKEKTNMLISMLDEGLIGDI
                     FSNVSKKSQHL"
     gene            complement(19286..19942)
                     /locus_tag="PLJ75_06535"
     CDS             complement(19286..19942)
                     /locus_tag="PLJ75_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04911.1"
                     /translation="MITMQQLEELESRIIKALQLIGDLRAENAQLEANNESLKVQAEE
                     ARLSLEEKEQEIARLKKEIDKATNELSQIKQKEEVLEKKIVEMLGKLDTLKSGIPSYQ
                     AKPAPDTASTKAQPQQKPSLKAEPEPRAEEDFMEDRGDDEDIIIIDDTETAKTDIEVE
                     TLPTEEDEIVLLDDTDDEIIIDDVNDDLIIIDDDSAEKKSKDKTSDDDDFIIIEDEEK
                     "
     gene            complement(20156..>21074)
                     /locus_tag="PLJ75_06540"
     CDS             complement(20156..>21074)
                     /locus_tag="PLJ75_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04912.1"
                     /translation="MIQIKSAILMALFYVIFIHGNTIVSAPLTPSIYTVNEGMVEGSV
                     STDYVSAHSSFMKEKIVLNLGVMPKTNLRFTAGWLQIIDNNSLSSSTIGDSSIGIWHS
                     FGSLFNNFILGYYIDIGLATGQNAFNDETKYPTESGFSTVSWGLVGAYTVHNVTFIMA
                     GKYELMPEDSSSVFDGLTFNVADSNTWKRFFGLNPFSKDAMLYYKKFKNDIIAFSLTA
                     LYNGYPVILYMSLSSGNRLNNRPVYGDHNLPILYKGYNDTIFMVVASGARYFFNEKTY
                     FGAFLVYNIIRDYSYAVKYSLGIEVSSLW"
BASE COUNT         6309 a         4385 c         2963 g         7117 t
ORIGIN      
        1 acagtatcat ctgatgtaat gcaacgcgcc ttattggcat ttcactttat cagagaatca
       61 acgttactga tgcttgataa tcccacacag aacctttcat tatcttcata cactatattg
      121 cacaatgcta tcattaaata tacatcttca gggaaaaatc tttgtattat agcctcacat
      181 gatttgaatt ttttaaccca aactacagat aaattatata ttatggatac tggaactatt
      241 gccagctggc accttcctgc tgatataaat gaaaccataa taaaaaaata tctgggtgtt
      301 gatgtattta taacacgtaa tatctataat gggaagccca atgtacattt atattttgaa
      361 tcgtaactta catcaagttt acaggtaata ttctaatttt cttaatcctt cttccacact
      421 attgatgagc aaaagatcat atgactcagt aacatttatt aaaccatgaa gattttcgga
      481 tgcacccagt atcatgacac gccgtttttt ttggcgtgct aggtgtgata catcaattaa
      541 tgctcgtaat cctgatgatg ttaccatcga agtagcggaa aaattgatta taacattatt
      601 ggtagcgatt aaactttcaa ctaaatttat aaattctcgt tcagtagcag aacacaatac
      661 tcccgataga tgaataattt ttgccccctc tctttcttca attgaatagt ttatttctgc
      721 cgccatagta tcacaccatt gttaaataat aattcaatgc ttcagttatc aatgtgccag
      781 gcacctcctg gcctatcact ggcttgccca ccttttccag aagaacaaag aggattgtac
      841 cttttatgtt ctttttatca tactgcatat gatctattat atcactggta gaaaatgaaa
      901 tgtccggcag tagcttatag tataagagca attcctttat catttcaaaa tcttcttgcg
      961 gcatccccat cttcatagat atatacgact ctaccagcat ccctaccgcg acagcttcgc
     1021 catgacttaa cgtataatga aacactgatt caatggcatg cccaactgta tgaccatagt
     1081 ttaaaataga ccggatattt ttctcaaatt catcctgttc aacaatgttt gctttaaaat
     1141 gcaccgaatt tttcacacaa tgataaaggc tttcattaac attgccaatt gaaacctctc
     1201 ttctaagaaa aaggtttaac gaagaactat cgccaattat agcatgttta accgcttcgg
     1261 acatcccaca cttccactct tcaactggca gtgttgttaa aaatcccata tcaacaatta
     1321 tataatcagg ctgatgaaaa tgacctataa tatttttacc ggcttcaata ttgactgcaa
     1381 cctttccacc tatacttgca tcaaccatcg caagcaacgt tgtgggaaca tacacaatat
     1441 ctatacccct catatatgat gatgccagat atccggtaaa atctgttgtg actccgccac
     1501 ctataccaat gagtaatgac tttctggaaa attgattatg caataaataa ttaaaataac
     1561 tttcagcata cgcatatgat ttattatatt ctgtatcgtc atatccttta atgcaggcat
     1621 tgatttcttg taagctatca tcaatagttt gcctgtgaag taaatacacc ctgtcactaa
     1681 taattgctcc aataacatcc cattttgaat atggcaataa acgaacaaga aaattaaaaa
     1741 taccgtgtcc gatagttatt gtcgattgtt ttgatgaata atgagctaca attgtttcac
     1801 tatttatttt ggaatccaca atgaaccttt ataaattttt tcatttttgt ttttcaaaag
     1861 ctggatgata catgtgttaa gaggaggata aatgacaatg gtatctattt cctgtatggg
     1921 tttaaaacta tagccataca aaattttttc attcccaagc tgtattgact cacttacaag
     1981 gttgcaccag aaaatgacat ttattatgtg tcgcctacca tttggctcta tggaatcagc
     2041 aatcatagct atatcgccga cctcaacgtc aagacccagc tcttctttaa attctctttt
     2101 taatgcatta tccagtgatt cccgatattc aacgccccct ccaggcagca accaatagat
     2161 gtcgttccct tttttatgtg caatcatcag tattgtatca tcttttacaa ccaatccggc
     2221 aacacgaatt cttatctttt tatgcgatag tgacagctta aacctcatat gtgtatcctg
     2281 tcagaataga tgagctctaa cgctttttta gcagcatctg attccgcctt gcgtttactg
     2341 ctcccacttc ccatggtaat atcctcatca ttaatgacca gctttacagt atatattttg
     2401 ctatggtcag gtcctgattc ctgtataaca gtataaattg gccggttttt gtacttcttc
     2461 tggactattt cctgtaatgt tgttttggga tcacgaagat aggaaagctt gtcaatcctc
     2521 tggatatcct tttttaacaa tcgtaacacc aatttttttg attttgtcaa acctgaatca
     2581 atgtataccg caccaataat agcttcaagc gtatttgcaa gtatggattg cctctgtcgc
     2641 ccccctgtaa gctcctcacc cctgcccata aaaataaaat cacctaaatt taaattgata
     2701 gcaacgtttg ctaacattac ttcggatact actgctgatt ttattttggc taaatcacct
     2761 tcaggataat tttcaaatga tttgaaaaga tattcattga ccactaatgc taaaactgaa
     2821 tcccctaaat attctaatcg ttcattatct tttactggaa ctggtgattc atttacaaaa
     2881 gaacggtggg ttaatgctcg tcgtaacaat gatttattat taaatgttat ttgtaaccgt
     2941 ttttcaagct gttgcaattt gcgattttgc cttttttttt ctgatccctt cgttttgtca
     3001 tcattcattg tatnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaattttt
     3121 tttatttttt caaaatcaac tgccattacc tctgcccctc cttacctcgc ttgtttagca
     3181 taccaaatcc cttacaatat agacatatta gcgatagtag tatgaagtat ttaattttgt
     3241 caagtttaat atagatggag cgttatcaac gccccatgaa ttttttgaaa taacttttac
     3301 ccctttgtaa cttttacaac aggttctatt acaagttttc ctttataatg tccacattca
     3361 ggacaaactc tatggggtaa aatataggcg ccacacgtag gacatggccg taaattgggt
     3421 atccctattg catcatgtga acgtcgcatt cttgattttg cttttgattt tcgcctcttt
     3481 ggaaccgcca ctttcttact cctttttcaa attgtcaaaa ttaaaataat gctttcaaaa
     3541 aatgatacta tttatgtcaa tgctttttta ttgcatctca ttatttctgc ccgctttcaa
     3601 tagctccaag aaacatacca tactgcatag ctcttttagc aaataccgat tgtggaaatt
     3661 caacagtcac ttttgtaaaa tattcccgtg ctttaaaata ttcaccctta agactataca
     3721 tcctgccaat attaaaaaga atctgatcat tattatatga cgtattatat tttgattcac
     3781 ataacaaata gtatcgcaaa gcttcatcaa tattatttat atattcacag gcaattcccg
     3841 cttgttgcaa tgcaagtgga gcaaacagcg gcgaacaatg gcggtaggct ttttcataat
     3901 atttaactgc attgctgtaa tctttttcat taaagtagag gcttgcaatg atataatatc
     3961 catttttgtg aacataccca aaatatgaac tatcaactat tgatccaagc ttattgacgg
     4021 tgcgcttaaa tttttcctgc ttttgaattt cgtcaagtgt ataagtgttc ctatattctt
     4081 ctagaacagc ttcaaagtca gccgtttctt tattaatttt ataatcacaa aaaacaatac
     4141 ctgcaattac caagaacagc actataccgg aaacaattga aatttttaca acaattgatt
     4201 gatgtgcacg aatataatcc cgcacattca taagtgtttg ttctacaata tttctcttta
     4261 cactaacctg gcgatcaatc ataaggaatc attccttgtc taatttattc tttaacagct
     4321 cgcccaatgt aacagttcct gctgttggcg tgttcaaaat ctttttgatt tcttcttttt
     4381 cgctcattat ttcataatgt ttaatactga gcgataatct cttctttgca acatcaacgc
     4441 ccagtactac agcattcacc ttatctccaa tcttaaaaat atcttcaaca gaatcaattt
     4501 ttttccttga aacttctgaa atatggacaa gcccttctac tgaatcacca tcaatcttta
     4561 caaaaatccc aaatggtacc ataccggtaa ttgtccctga aacaggggta cgcggcttat
     4621 atttatctgc tattatttcc catggcgact ttgtcagctg ctttatgcca catgcaattt
     4681 tttgctcttc tttgttaatc tcaagaatag tacactgtac ttcctgtcct tctttatact
     4741 gtgaatgaat atctttaaaa ttttcatccc atgatatatc agaaatatgt attaagccgt
     4801 caacatctgg ttctaattca acaaacatac caaaattaag aatttttttg attggcctgg
     4861 tatgaacact ttttaatggg aagcgctcct gtatagtatc ccatggattg ggataaagtt
     4921 gttttatccc tactaaaagt tttcggtttt caagatcaac atcaagaatg aatacattta
     4981 ccctatcacc ttttttgtat tgctctttag gattaaccat ccgttttgtc catgaaatat
     5041 cagttgaagc acaaaaacct tcaacccctg cctcaaactc tataaacatg ccaaaatttg
     5101 taagcgttac caccttccct tctgttattt ctccaacctt gaatttttca ggaacagata
     5161 accatggatc agaaataagc tgctttaaac caagtgcaat tttgccttct gctctattaa
     5221 aagacaataa aacaaaatca cgttcttcac caatcctcaa tatcttcttt tttttaaaaa
     5281 ccttccgcca cgaaatatca ttgcggtgaa gaagtgcatc aataccacct atatcaataa
     5341 atgctccaaa atcaacaaac tttgtcacct tcccccgtat aacatcacca attgagtaat
     5401 ttttctctaa attgtcccat attttttgct gctcttcatc aagaaaatct tttcttgaca
     5461 aaacaacaga tcgtctgcgt ttatcaacat ttttaacttt aaaatatacg cttgaatgtc
     5521 cattttgcga tttccccttg acatcagcag ctaacgtcaa aggcaaaaaa gcatgaaaac
     5581 catcacaatc gacaatataa ccgctttttg atttttgtgt tacaatcccc tgtattatag
     5641 agcttccttc agtagccttc aaaaattgct gccagctttt ttcccttttt gctgctttta
     5701 ttgaaaatac atacatccca tccagaaggc gtctgtttac cagcataacc tcaacaatat
     5761 caccaacctt aggagcaatc tcaaactctt caaggcttac ccttccatca gtctttgtgc
     5821 caatactaac atatgcgaag tcattatcaa cagttactat ctccccatta taaatagtct
     5881 ttgtttgaat agaatcattt gaaaggcttg aaacatgatt catatatagc tgactatcac
     5941 ttctctctaa attcatatca cttcacctat attcattact tttacacaat tatacaatga
     6001 aaggaattac tctttataag tcaattttat tttagcaata atttaaagct atatccaaaa
     6061 attacattat ttgtatagaa caatatcaca ttcataaata tttaccatca ttctgtacca
     6121 ctatgacaat agctaacagt attgtatttt tgtattgatt aaagaataaa tgctatgaac
     6181 aacctcaccc tgtgtcatat tgcttgtatc aaggtatatt gcgtcatccg attttttcaa
     6241 tgcaccaaat tttcttttca tatcctgctc atccctgcgt ctaatttgtg ttttcaattc
     6301 atcaaggtca acttctttac ccttcaggga atactctaaa gctctgcgtt ttgctcgctc
     6361 atcaactgag gcatccagat atatcttgat aagagcgtca ggaaaaacaa ctgttcctat
     6421 atctcttcca tcaacaataa tggaattgcc cttagcccat tcacgcatca gggaattaac
     6481 aaagtttcgt acttcaataa tatctgatat tttcccaata tgtgatgcta tagtttcact
     6541 acgaattttt tcactgacat ctattccatt acagatagtt gtacaacttc catcttcatt
     6601 aaacacctgt tcaagatgta tagcattgag gtgcctgcat acatcatcac gagtgaatga
     6661 ctcaccagat ttttccagaa catataacgt tatggctcta tacagagctc ccgaatcaat
     6721 atactgcaat cccattcgtt tagctacttc acgcgcaaca gaactcttac ccgatccagc
     6781 aggtccatct atagcaataa ctatattttt attcattata tttttcccat ttttggcgga
     6841 tagtagcaaa atacctgtac agcaacgttc tatcattggt ttgtgcagcc tgttgcatta
     6901 tgctttttat atgcaaaagt gaattgatat atgaatcaag tgcattcaca atattaccat
     6961 gattgagtaa aacaatatct gcccacatat ccggtttgcc gccagctaat cttgtcatgt
     7021 cgcgaaatcc tttgccaata aatggttcta tggtgctata ggtattgttt gtaatgctat
     7081 caaaactatc aactaatgca gtggatgcga tatgaggcag atgacttgaa tatgcaactc
     7141 ctgtatcgtg cagttgtgca tcaataatga tagtgttaca ttctaacatc ttccagaatg
     7201 cttcaatttt ttttatatca ctttccttat ttttatcatg aggtgttatt ataacccatg
     7261 cattgccaag cagagaatgc gagctgtatt ggtacccgga tttttcagaa ccagccattg
     7321 ggtggcatcc aataaactga caggctttat catgcgcaat gattgcatca ataatagatt
     7381 gttttacact tcctgcatct attattaagg cttcattttt caaatcatca ctgctcaaca
     7441 atgactgaat taaatcaata gatgaaagaa caggtgttgc aacaataaat aaatctactc
     7501 cactacaatc tattttattg atggagccaa tattgtcaat aataccttct tctttagcac
     7561 tttgcaatag ctcaggattt ctgccatatg cgctaataac aatttcatga ttttttttct
     7621 tgagcatcct ggcgatagac cctccaatca gtcccagacc aattattgca actgtattaa
     7681 acatcaatac atacctcttc ccaaagccag tgcaacactg gcaagttttt tctttaaatc
     7741 aaccagttgt tcataattaa tggtttgttg tccatcagac attgcttctt ctggattggg
     7801 gtgcgcttcc accattattc catcagcacc tgctgcaaca tatgccaaag ctgcagctac
     7861 aacataatct ttaagcccca ttgcatggct tggatcgcat ataacaggaa gatgcgaaaa
     7921 atctttaagc acaggcactg ctcctgcatc aagagtatta cgggtaacat cttcaaatgt
     7981 acgaataccc cgcgggcata aaataacctg cttattcccc tgtgaaagta tatattctgc
     8041 cgacatcaaa aattcattaa tagtggcact cataccacgc tttaaaagaa taggcctatc
     8101 gattttaccc aactccttta ataaattaaa attctgcata tttcgtgttc ccacctgcaa
     8161 tacatcggca cactcagcta ttaatccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnact gactcaaccc ctgaaattgc ttcaagcggt tgttcgcgaa tgatatcctc
     8341 atcaccaata acagtaataa tagtacggta ttgtcctttt gaaacatgtg gctgtaaatt
     8401 tagcgatgtt atctttccaa caatatgatc aatttcatct tgtgttatat taggttttaa
     8461 tacaataatc atttagcaac tcccccttaa caaaaaatat tttattcatg cctctcagcc
     8521 tgatatgatc ccaatatctt caaaaatata gtttcctgtt tcatattttc taacgcttcc
     8581 tgtaccaccg gatctgttct atgccccata aaatcaataa aaaaattata tgcccacatt
     8641 tttcgtttat caggacgcga ttcaatcatt gtcatattta tgccatgatc gctgaatggt
     8701 tttaacagtt tatataaagc tccaggctga tctttaactg cacatactat cgatgtctta
     8761 tcattatttg aaggtggatt agcatgttta cctaaaacta aaaagcgggt aaagttttgt
     8821 cgtgaatctt caatcccatc agcaataata tttagattat aaattttggc agaaatagtt
     8881 gaagcgatag ctgccgaata tttatcccac gaagctgttt cagcagcttt tgccgttgaa
     8941 tttagctgaa ttatttctac attgggtaga tttgctttaa gccagttttt acattgcgcc
     9001 agcgattgag gatgggaata aaccttttta attttatcga tattgtcaca tactgacaac
     9061 aggctatagg taatacgcaa atatttttca gaaacgatgt ttaaatctgt ttcaagaaat
     9121 tcatccatgg tataggtcac tgcgccttca gttgtattct caattgggac aacaccaaat
     9181 gtcgctctgc ttgtttcaac ctcatcaaaa acatcagaga tagttctatg agctatcgct
     9241 tcgatggcat ctccaaaaat ttccagaagg gcaatgtggg taaacgttcc tatgggtcct
     9301 aaatacgcaa ccttgatcgg ctttacaact gatactgcca gtgaaaaaat ttcagttgct
     9361 atcttttcaa ctaattctat agttattggt ggcctggcat gttgcttaag attcgaaatc
     9421 atctttgctt ttacagcaaa tatgccctgt tccccatctt gaacaatatc ctttaattct
     9481 tttatatacg tatcaacata ttgagcaatt gtttcaataa cagtattttc aaaattatta
     9541 ttctccattt cactcctctt caaaattata ttcttttatc tcggataatt ttggtaaatc
     9601 tgacagctta ttcaatccaa aatatttcaa aaactcatca gtcgtaccat ataataaagg
     9661 tcgtcccgga atatcttttc ttccaaccgg tttgatgaga ttttttcgca tcaaagctgc
     9721 cagcatcatt ctagaagata ccccacgcaa ttcctcaatt tctgcaagca taattggctg
     9781 tttataggca atgatagcta atgtttcgag catccctttt gataatgttt ctttcttctt
     9841 ataccccata gcctgccgta tggtagcagc aaatttagga tttgtaacaa actgatatcc
     9901 gccagatatt tcaacaagct taattcctct atctctgtct tcatattcat caatgagcga
     9961 atcaataatt atttttgcct gtgtagcatc tattcccatt tgctttgtaa aataattaag
    10021 cggtactggt tcagaggaga taaaaagaat agcttcaata ataccaaaaa tatcacctcc
    10081 ccctgacgtc tcttcagata attcgtcctg agaactaatt gcatcatctt cgtctatttt
    10141 ttctaaattt tcattttctt gaatgctatc atcattcata attattccct gtatcaatgc
    10201 tacctcaata tattaattca gctttttcca acgatttttt aaaaatatac atattgccaa
    10261 attgtgcatt ttgtaatact gctattgcac ctatacggat catttccaat accgccagta
    10321 acgtaaccac cacttccatc ctggcaggtt tttgaggaaa tagctcatta aactcaatct
    10381 gctctgtatc ctgcagcatg cttattaaca gtataatacg atcactaaca agaatttcat
    10441 caaatactat gctctggctc tcaacctctt tttcagattc aaggacatca acaaaggcat
    10501 tgagaagatc aaaaagattc acttctaaat atacaacttc atcattgtta accggattag
    10561 gttgtcttgc aaagacatct gattgcattg catgtaatga aatcaaattc tttgaagcaa
    10621 tctgatattt cttaaattca agtaattttt gtattaattc aggaggcaaa ggcggaacaa
    10681 aaaattcatc tccaatatcc gaaccaggta atagtgcttt tgatttatag taaataagct
    10741 cacttgccat aacaataaaa tccatagcaa tcggaatatt tatcttctcc ataagggaca
    10801 gatagttaca atattgatca gtaatttcag ctatggaaac ctgcgttatt tctattttag
    10861 acttttttat aagctcccat agtaaatcca gcggaccttc aaaattattt atatgcaaaa
    10921 tacattgcgc ttcaatttcc tgtgatgaaa ccatgattta accagaatta taaatagttt
    10981 ttattgtaaa aaaaagacta ttaatgacaa tctttttatt tatgcaacga taattaattt
    11041 ttattattta aataatacaa gtttgcaata attccttaat tcattctacg atgtatttat
    11101 gctaatgcct tatctgcagc tttttgaagt ctttcgggag ggaatggttt cactacaaaa
    11161 tctttaactc caatttttat tgctctggtt aataaatttt cctgacccag agcggttacc
    11221 attataatac gtgcattggg atcaaacagt aaaatttcct cagctgcttc aataccatct
    11281 ttttcacgca ttgtaatatc catggtaacc agatctggcc gtaatttttt ataaagctga
    11341 acagcttcaa caccattttc agcctcacca ataatttcat gccctcttgg gaccaatgca
    11401 tctttgacaa gggttctcat aaatttcgca tcatctacta ttagtatttt agccatagcc
    11461 ttaccccaaa gtatataata tcccacatcc acacgccggt atataatctt aaatacatat
    11521 taccgataag cctatttcat tcaagaaaaa atttttatta attacaattt tttacaaata
    11581 ccctcgaatc gaatactctt ataaacatta accgtataat aacagctata ataaacaact
    11641 tatatacgag ctactatttc atcaggtatt gaatcaagat caacaactgc atcaattgcc
    11701 cccatttcta ctgccaccct attcataccg tataccactg aagttgattc attctgtgca
    11761 atagtatacc caccattatt atgaatttct ttaataccca tggctccatc tcgtcccatt
    11821 cctgtcatta tcacccctat attattttcc atacctactt taaccatcga ttgaaatagg
    11881 acatctgctg agggtctgtg acctgatacc ttatcagact gtgacaaccg tattatacgt
    11941 ttattattca ttttttccat caccatatga gaatctcccg gtgcgatata tacacatcca
    12001 tccatagctt cttcactatc ttcagcttcc ttaacagaaa catgacatag actatccaat
    12061 cgttcggcaa atgcctttgt aaatcctggg ggcatatgtt gtacgacaag tattggtgaa
    12121 ggaaagtttc ttggtaattt ttgtaatact cgttgtaatg ccgatggtcc accagttgaa
    12181 gtgccaatcc caacaatttt tttaaccctt ttttgattac tatcaaccgt cagctttgtt
    12241 gattgtctat tcactatatc tttgctatga gatatcttca ggggtatgcg tgctacagct
    12301 tttactttcg atataagtaa ttccgcaata tcatccacgc tcattgccag aagatttgaa
    12361 ggtttaggaa taaaatcaat tgccccaaat tcaagtgctc taaacgttgc ttcagcacca
    12421 tattgcgtta gcacactcat cataatgaca ggttttggat tcgtctttat gatttcctgc
    12481 aatgcttcaa gcccatccat tatgggcatc tctatatcca ttgtaattac atcaggatta
    12541 agagtcagat ttttaaatac tgcagatttg ccattatttg cagtgccaat aacctctata
    12601 tctttgtcac tttcaagaat atcagttatg acttttctga cgagagcaga atcgtcaaca
    12661 accatgacct taattttttg tgacatagag attaatttcg caacctttca actatcgtac
    12721 ttaatgatga agttgttggc attatcataa gtttaccaat cactctgtca tcctgatgga
    12781 aaaattcagt atccatcacc agggcatatt cattttccgc tgtatgaaaa gccagaatgg
    12841 aatcaagtat tgactgcatg taatcatgaa caactgatgg aacagttggc tttattgcca
    12901 tttctatttt atcagcaaat gcattaacaa cagccgaacc aacaatattg gtaacctctt
    12961 taaggactga ttcaccatct tcgcttaatg atgtggtagt accctgaggc aacccaagca
    13021 gcatatcaac aagttcataa ccggattcct ggtgcaaact gaaaaggatg gtaccattaa
    13081 tctgtccaac aattggcata taaacaccaa gataaacact gtcaatatca ccaaagaatt
    13141 gagggattgt ttctatcgcc agtactgata catccggtat ggacagatca actcttctgc
    13201 ccagtatttt tgaaagtttt tctgcagcat cagtaatccc aacatttgca agaacctgaa
    13261 ctttggcaag atcatcatct gatataccta atttattata aattttgttt tcttcaccaa
    13321 gggttgtttt tattttttcc ttcagctcag atttaaactc ttcaagggca ttgtgaacct
    13381 gtctttcaat tatattgttg tttgtacttt tttctgattc ccctactcga gtttcttcta
    13441 tttctacaat ctctccattt agttgtttct taaaaataat atcctcttta atgggcatct
    13501 cagtagctgt aacagattcc ctgatatcct ttttatagtg atcatcatgt tctggttttt
    13561 cagcattgtc ttttgttgct tcatgtttta caacctccac tgatgcctgc cttgtatcca
    13621 gcactcgctg tttttcttta tgcgacagca tatcctgctg cattattact ttattaatca
    13681 tagatgatat atcaagtata aggcatatac ttccatcacc caggatagag gcacctgcaa
    13741 gccccggaac tgttttataa ttttgctcca atgatttaat aactatctcc tgcttacctt
    13801 caagcaagtc aacaactata ccaatttttc tattgccgta gcctacaaca actaccggca
    13861 ttctttcatt atcttttaat atgctattta tacccacaaa attatttaag cgtatcagcg
    13921 aaagtatttc acctctaaga tttataacct catgtccttc aatggttgtt atctcatcag
    13981 gcaatatttt gatagtctca attacatctg aaagtggtat cgcaaataat tctctttgta
    14041 ctctaaccat gattgcaggt atgattgcca gcgtaagcgg aaagcttaaa ataaaacgcg
    14101 tacccattcc aggctcactt tcgattgata cgctaccatt taattcatta acagtctcct
    14161 gcacaacatt cataccaaca ccgcgaccgg aaatatcggt aactttttct gaggtggaaa
    14221 aaccaggcat aaaaataaag ctggctaatt catctttatc catattcgca actatttccg
    14281 gtgtagccag attcatctca atagcttttg attttatttt tttatagtcc aatcccctgc
    14341 catcatcact tacctctaca aatatctgat tcccaccttg atatgcatta agggttattg
    14401 tggcgacatc tggttttttc aactgccgtc tttcttctac cgattcaata ccatgatcga
    14461 cagcatttct gataaggtgc atgagtggtt cgcctataac atctataact tttttatcaa
    14521 gttcagtttc ttcccccttt atttccatga taactttttt atcaaactct tttgctaaat
    14581 cgcgaaccaa cctattaaat cgtgtaaaaa cctgaccaat gggcaccatt ctcgttttca
    14641 ttattccgct ttgtaaatct tttgcaatgc gggacatctg ttccattctg tttttaaatt
    14701 cattaataat agtcttttca acagaaaatc gtcgcatctc ttcatacaat ttgtaaaaac
    14761 cagaattagc tataacaagt tctcctacat tattcagtag ctgatccaat ttatctactg
    14821 aaaccttcac tgtcctgagt tgaactgttt tcttatcttc ttttgatcgt ataataatct
    14881 catctttctc ttcctcatca ggcaccatgt cctcatcaaa atccggttgt ttaggagcag
    14941 cttcttgttt ttcttcaaaa agcgtttcct gagggtgata ttttaatgca accttattat
    15001 ctttttttat cagctcaatt ttcctcaggt ctatcctgat gacctgatca acttcgcatg
    15061 ctttcctgat ctcatccact gacgcattcg taagtaaaat tatctgaaca caattattga
    15121 atccaggcaa agcaacctct tcaatagcag gaatagtcac aaggacgtcc cctatctttt
    15181 caagattggc aagcacaagc tggactttta atgatttcat tgcagcatca gtatcaaggg
    15241 taatactaat ttcacagcat gataggccgt tgtttatgcc atcacggata tcttttacta
    15301 tactagctgg aagctgaatc ttccttgtta caggttgttc ttttttttca gcaggattct
    15361 caaaatgagt agaaactcct tcaatttttt taattaatcc tgtaaaatct ctggtgggtt
    15421 gatttcccgc tgaaacttcg tgtataactt cattaatgac atcaaaacat tggaataaaa
    15481 catcaacaat tgaaggtgtt atttccatta ccttatcgcg aatgccctgc aataaatttt
    15541 ccattttatg tgccagatca cttagatcag tcaacccaac aaaagctgct gaacttttta
    15601 aagagtgcgc agctctaaaa atattattta taatatcctc atctgaagga tttttctcca
    15661 gttgcagcag gttcgcatta agctcctgta gttgttcatc agcctcttct aaaaaaatat
    15721 cctgatactc acccaaggaa aagcccatcg ttttacctcg aactaatatt tagtttttta
    15781 catgctactc ttcaccaaac aggatattgt ctaaacgtaa tataacaata agcctgtcct
    15841 gtaatctccc tataccattg ataaattcgg ttgaaatgcc ttcaattaat tcatgaggct
    15901 catcaatcct tgaacgtgaa agccgtatta cctggtagac attatcaacc attaacccaa
    15961 ccaatttttc tccaatttca acaacaatta ttctgctcaa ttcggtcact ggtacagatg
    16021 gcataccaaa gcgcatccga atatctacaa cgggtattac attgccacgc agattaataa
    16081 cacctttaat atactctgga gcattgggaa gtcgtgtaat ttcaggtata cgaagtattt
    16141 catgaactga taaaatatct ataccatatt ctattttatc aagcttaaaa ctaaccaatt
    16201 gtattatatc aagcgaatca ttagtatcaa taaccgattc ggctgttttt gctgcaatac
    16261 ttattagttc attattcata tatcacctgc ttatactata aaagtactac aatttcaata
    16321 atagtaaata ctatcataga gcactaatat aattcaacac aaaaatattc aatacataaa
    16381 aaagtttttt attttcaaca cagcaataca ttggcaatca gcatgacagg caaagtcaac
    16441 aatgaatggt taagtcagaa aataatagtg taagcttaca tttatctatg caatttttgg
    16501 tagttattac tgtagaaaat agagtgttca cacctttaaa atccttttac aacctatccg
    16561 gcaattaaaa caaagcaatt gtatcatcag tggtgttcac aaaggtatta caaattagaa
    16621 tcttaaatgt tccctatatt ttcagggtta gcaatctatt ctcaatacaa ataaatacca
    16681 ttttaggagt cactgctact attgttgcat caggagaagg caatgtatat gaccataaaa
    16741 tctcaccatt tgatgcaatg agataaaaaa ctgtatcggc aatacatgtt atttcattgc
    16801 ggatgctttt taatgaatgt atgctcttgc ccggactaat cttccattca ctatccccac
    16861 tggcagatag cttacacaat aagccattcg ttgtagcata tactatccca ttatcgtatg
    16921 ccattgcatc aataatacta ctgtcgttga atgaccatac cagttcgtct tttaaaacat
    16981 gcaacttctg aattgaatca ggagttacaa aataaatttc aggatactgt gctataagcc
    17041 cattttcaat aacagtgcca atgtgtttac ggtagatgct aacacccagc gaaatatcga
    17101 ttgcataaag gtacccatca ttcgtcacaa tacaaaccat ttgattaaat aannnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncccatcgc tggttgtaac cagcacatca
    17281 ttgttatcag gtacaacagc tttaatatta tacagggcat tgagcgttag ctcattttca
    17341 ttcacaggta ttgcttgatt aatatctctg gtcaacgttt caataatctg gattattttt
    17401 ttatcggcga tagttaccgg taaagactgc tgtataattg tgtcaagttc tgtagtattc
    17461 atggaaaccg ctttttttat agcaatgatg gtacccgcat caacaacaca tgcttgccct
    17521 acatcaacaa caacaccagg agtactttct tgtggtatga tatccaatct gggagcaatc
    17581 atcaccttcc cttccagcac tattaatttt gttatattat tattggactt tatcataaaa
    17641 gttgtaccac gaatacttgc aaccgcggtt ttagttttta caaaaacaga atctcctttt
    17701 ttaagttttg caatagttgc atagataacg ccacgcgtca ttgacgtatc cagtaataat
    17761 ttacccttgc tctgtactgt atcgatggca aattcagaat tttgatctat ccgtaatttt
    17821 aaactcccaa tttctataac agccattgaa cccttaccac atattatcct gtcccgctgt
    17881 tgtattccca tgccattcat caacggtacc gactgattac ctcgtataac atggacatct
    17941 ccgattactg ccgtgcatac tgccttttcc atagttcccg aaaaataatg agtaaaaagc
    18001 attactccag caataactat cgccgctata ccagttgcaa acatagcaac aagctttttt
    18061 atttgtatac catgagagcc agtttttgct ttttcaatga tgtcgtttga agtaagtgta
    18121 tcctcaagtg ccggcaatga agactgccat tgccggatta tttttagcaa aacatccgat
    18181 gctttccctg catacggcgc aatctcatgt agcaattcgt tgtcatcaat ttgtctattc
    18241 ataacaatcc catgtgtttt gctgtatgtt tcaaaaaact atctatcata tactatttca
    18301 tatgactcct tttttcttta aatgatcagt aataaaggca agcgcccttt ccatacgcca
    18361 ttttgcagtg cgttctgaaa tatccagaat atgtgcgata tcgctatatt gcaattgctg
    18421 cccatatcgt aataaaaata cctctctgaa tttattattc aatgcatcta ccgcctttga
    18481 aaactcttgc aacaattcat tttcaataac atgatcttca ggcgttttca tggcatcagt
    18541 attcattata cgctgctcct cgcgctcaat catggtatca tttttatata ccttacagcg
    18601 atagtttttg gcacaattta ttgctatggt atataaccat gcggaaaaag cccttctaac
    18661 atcaaattta ttcaaatgcc tgtatgcccg tataaacaca tcatgcatca aatcaaccgc
    18721 atcatctaca ttataaacat agctgtacaa aagtttaaaa attcgctccg aatagcgctc
    18781 ataaagctct tcaaatgcac ggtcattgcc atgcattacc gattgtacca attcttcatc
    18841 agatagagct tttatggatt ggtccattgt tattataata tatcaccggg ataggataaa
    18901 agtgcaaagc gggttttatt ttttttatat cttttcatat caatctctta tagatgctgg
    18961 ctttttttgc ttacattgct aaaaatatca ccaataagcc cttcgtccag cattgagatg
    19021 agcatattgg ttttttcttt tatttcatcg ctcatcatat actctgattc tttaatctga
    19081 aataattcat cagcaatatt caatgctgcc agaatagcaa tctgcaaaga gtttgcatgt
    19141 gatatagtgt tagctacatc ttccattttc tggttaacat aatcggcaac ctttataata
    19201 tggtcgcctg atgcatctcc ttcaatggtg taaacctgtc ctaatatggt cactttgact
    19261 ttattaggat tatttgcaga agatatcatt tttcctcatc ttcaataatg ataaaatcgt
    19321 catcatcgga agtcttatct ttagactttt tttctgctga atcatcatca atgattatca
    19381 aatcgtcatt aacatcatca ataattatct catcatcagt atcatctaac aggactatct
    19441 catcttcttc agttggtaaa gtttcaactt ctatatcagt ttttgcagtc tcagtgtcat
    19501 caataatgat aatatcctca tcatcgcccc tgtcttccat aaagtcttct tcggccctgg
    19561 gttctggctc agcctttaaa gatggctttt gctgtggttg tgctttagtt gaagctgtgt
    19621 ctggcgcagg ttttgcctga taagaaggaa tcccggattt tagcgtatca agtttaccta
    19681 acatttcaac aatcttcttt tccagaacct cttccttttg cttaatttga gacagctcat
    19741 tagtagcttt atcaatctcc tttttaagcc ttgctatttc ctgttctttt tcttctagac
    19801 taagccttgc ctcttcagcc tgcactttaa gagattcatt attagcttct aattgtgcat
    19861 tctcagctct tagatctcct ataagctgta atgcttttat aattctgctt tcgagctctt
    19921 ccagttgctg cattgtaatc ataagaacat cctcccgaat tatcattttt ccatgtatca
    19981 ctgctttaaa tgtcggtgaa aataaaattt tagtaaacta aaatttaaaa tgacatcatc
    20041 aataacaata aagtataatg accaatatat aaaatcaaga gtttttttaa ttatttatat
    20101 aattcattta ctttctgaat ttttccccat agttttttaa gtaatgacag gaatttcacc
    20161 acaaagaaga aacctcaatc cccagtgaat attttacagc atagctataa tcacggataa
    20221 tattgtacac caaaaaggct ccgaagtaag ttttttcatt aaaaaaataa cgtgcacctg
    20281 acgctaccac cataaaaata gtatcgttat accccttata aagaatgggg aggttatgat
    20341 caccataaac gggtctatta ttaagcctat tgccagagct caatgacata tacagtatta
    20401 cagggtaacc attatacagt gcagtcaatg aaaacgcaat gatatcgttt ttaaacttct
    20461 tgtaatacag catggcatcc ttactaaaag gatttaatcc aaaaaatctc ttccatgtat
    20521 tgctgtcagc cacattaaat gtcaagccgt caaaaactga tgaactatct tccggcatca
    20581 attcatattt acctgccata ataaatgtta cgttatgtac cgtatatgca ccaaccaatc
    20641 cccatgatac agtagaaaac ccggattccg ttggatattt ggtttcatca ttaaaggcat
    20701 tctgtcccgt tgccaatcct atatcgatat aatatcccag aataaagttg ttaaacaacg
    20761 aaccaaagct atgccatatc ccaatggatg aatcacctat ggtactgctt gataaactat
    20821 tattatcaat aatctgtagc cagccagcgg taaaacgaag atttgtttta ggcataacac
    20881 ccaggttaag cactattttt tctttcatga atgatgaatg tgcactgaca taatctgtgg
    20941 aaacagagcc ctcaaccatt ccttcattaa ctgtatagat agagggtgtt aaaggggcac
    21001 taacaatggt attgccatga atgaaaataa cataaaaaag cgccatcagg atggcgcttt
    21061 taatttgtat catt
//
LOCUS       DAOOJH010000036        20686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2585_length_20686_cov_11.084872, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000036 DAOOJH010000000
VERSION     DAOOJH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_20686_cov_11.084872"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLJ75_06545"
     CDS             <1..237
                     /locus_tag="PLJ75_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD04913.1"
                     /translation="MNMLAEQDPHLDVQEPVTLRVTMQEIANWAGMQINDVQKLLAHL
                     NKLGKIELFNDRVIVRNIRDFQRMVNSRRKSMYA"
     gene            238..311
                     /locus_tag="PLJ75_06550"
     tRNA            238..311
                     /locus_tag="PLJ75_06550"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Arg,seq:cct)
     gene            374..1453
                     /gene="prfA"
                     /locus_tag="PLJ75_06555"
     CDS             374..1453
                     /gene="prfA"
                     /locus_tag="PLJ75_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPD04914.1"
                     /translation="MESRLQQLLDRYHEIEQAMADPKTVNDQNLFKSLTKEHSKLFPI
                     VQKYNEYTKIKKETEEVTNALKEENEPDMVALLEDEKIQLESKMNEIEEQITQLLLPQ
                     DPHEGKDIIIEIRAGTGGDEASLFVADLYRMYIRYADLKGWKVEFIDANQTELGGFKE
                     IIFSISGDDAYSCLKFESGVHRVQRIPVTESGGRIHTSAVTVAVLPEAEENDIQINPD
                     DIRIDVYRSSGHGGQSVNTTDSAVRITHIPTGIVVTCQDEKSQHKNRAKALRVLRARL
                     FEKLERERMEKETALRRSQIGSGDRSERIRTYNFPQSRVTDHRINLTIYDLESVLDGE
                     LDKIIIPLREYDMEQMLKASHVYHA"
     gene            1437..2306
                     /gene="prmC"
                     /locus_tag="PLJ75_06560"
     CDS             1437..2306
                     /gene="prmC"
                     /locus_tag="PLJ75_06560"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPD04915.1"
                     /translation="MYIMHNNATIFDILSQAAHRLASISATPALDAEILMAHTLNIER
                     HTLPLHYSTHISKIIYKKFENLLLRRLQSEPIAYIIGYKEFYSLPFHVNQHVLIPRPE
                     TELIVDFALYYAHHNATILDIGTGSGAIAIAIKYNLTDCSVWATDISADALRIAKKNA
                     ATLLGMNKIKFLQSDLFTSLPHVHFDVIVSNPPYINPEIYTTLQKDIFFEPKEALVAP
                     DHGFGIIKAIINDAYNYLTKDGVLIVEISDDLAHQIVNYTQSLPYTISIAKDYSGMQR
                     VAILHKTIKNISN"
     gene            2437..3348
                     /locus_tag="PLJ75_06565"
     CDS             2437..3348
                     /locus_tag="PLJ75_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04916.1"
                     /translation="MINGKEELLHDIRVANFIEAYRFSCISKRDKWYSFSNIEWDPIL
                     SEIKIEAIINFNDSSYTINQIIRQDTIPKKSIGNQRFKYTILEPLKESKIFFKESDIT
                     GTILIQNYFPYYDIIHHSSISPNNVENMFNFYSDRCKAKVSVELHKKNNTTHKYFDCV
                     GFREHSWGLYPSHHISCDSRVVVHFRDQTISFRYIEYNGMSYSYGCISKKSGNLALVL
                     VELEFLSIKNSSFESSEFTYKDAQDDIDLIVSKPIKPHHIEVSKKLKKSHLHIVSFSD
                     FTVIGTNKKGYGIEEHLISYERLKNVL"
     assembly_gap    3723..3822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3823..4161
                     /locus_tag="PLJ75_06570"
     CDS             <3823..4161
                     /locus_tag="PLJ75_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04917.1"
                     /translation="HNQKLQSAQHLAQLAARFADGKENIIIDLFEKGNMSFKIWAMNV
                     MQHHNKMIYIPPLLPHCTDINETMSSTAYSTITKITGKNFSSQLNLPYNNPKVLRAIK
                     DFYFQKNTIQ"
     gene            complement(4124..4759)
                     /gene="nth"
                     /locus_tag="PLJ75_06575"
     CDS             complement(4124..4759)
                     /gene="nth"
                     /locus_tag="PLJ75_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPD04918.1"
                     /translation="MIDRGTSKKIITRLKKEYPDVAPPLKYANVYQLCIAVVLSAQTT
                     DNQVNRVTPILFKKYGDFATLAAANVHEVEHIIKPTGFYHTKAKHIIALSQQVIEKFN
                     GNLPADREQLMSLPGIGRKSANVILATGFGIPAIPVDTHVARVAHRIGYCSSKKVYEI
                     ETMLMKSIDESEWITAHFLFIYHGRNTCMARKPQCSHCPITVWCSFENKNL"
     gene            complement(4752..5282)
                     /locus_tag="PLJ75_06580"
     CDS             complement(4752..5282)
                     /locus_tag="PLJ75_06580"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD04919.1"
                     /translation="MSSKTLTIFCAGLVLIHLQCTTTKNVKHQHHWRFNDPYINQTII
                     SGWEHINKRHYEWAMLDFTRLIQKNYIDYDILFGAGLACYYTNDTVKAIDYCTGAIEQ
                     NPQHFEAYFYRAQCYLALNNKKKALDDLHTIVAMKLNQPFICGYYINNDDLADDAALQ
                     ERKKEAQKIIVAIVHD"
     gene            complement(5272..5877)
                     /locus_tag="PLJ75_06585"
     CDS             complement(5272..5877)
                     /locus_tag="PLJ75_06585"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HPD04920.1"
                     /translation="MDVVVATRNNGKMKEIVAFDIPSVIFTSLDAYPEIGEIEETGAT
                     FTENALIKARYVCTVSGLPALADDSGLVIDALNGEPGVYSARYGNCATDDDRCRLVLS
                     KMINVPWDKRTARFICVMALVFPDGKEYIEEGICHGFIATRPAGDNGFGYDPIFYLPY
                     FDKTMAQLPLSVKNTISHRAKALQKVKNLLQTMVRQKSHEQ"
     gene            6123..6797
                     /locus_tag="PLJ75_06590"
     CDS             6123..6797
                     /locus_tag="PLJ75_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04921.1"
                     /translation="MEEKKQINILRIGVANLTELEELVKAFRLMNQYSKRRRFVVSRE
                     DLKDIYGNKIVEKAHDINISVVKLLQRNFKPDTVFKIFSSDEGIAIVTYFESMTGKDF
                     SMQLIETIGNIGGGIYKPFIDVVPNFHELFKLFEKGLSPKLVVVGYIPVKNVAQEVSM
                     YNEIKKFDPYIRLLDITNDDLKPKSFLKGAVLFHFDNTLKVWHRLLTKIINEYTKPYF
                     MEEISK"
     gene            6819..8861
                     /locus_tag="PLJ75_06595"
     CDS             6819..8861
                     /locus_tag="PLJ75_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD04922.1"
                     /translation="MDERKLQLFAPVILILTIITSISITIMLYLQKTNIIMIVLSCIL
                     SATLITLLIIFRLIHKVFFTNNIRKILSVVDNFKKGIFVQPSNEIKGNDLLSKMYGEL
                     LIASRHLDNIIKSQKNEIDKFRELYNNMMFSMNAYIMALDFNNKIIFTNELFLNKFQL
                     SPSEVNGKNLSDIFYFLTGTVLQAIEEIKSTHESIILEKAHLISHNRISIIADIKLSF
                     MISNNEPQIIMVMDDVTSKTKRDYQISLITRLSETIQKDDALDHILRTILTAVTSGTG
                     LGFNRAMLFLRDDKNNILYGRMSVGPDTLDEAFSIWSSLNPLKGKEILSRGINGDDVH
                     GRLLKEKVESTSFDLNEDTIFTQTLQSGETIHITDALHDQRVNDEIKNFIDVNEFVIV
                     PLIALNKIIGILIADNKFNQMPITQDSVDLLTVFAYQAALSIETYNNLELLKKEMEKI
                     RHRQEAIVESEKLAAIGRIASHIAHEIRNPLVTVGGYSRRILQTANAQQIDIQKIKKA
                     AQIIMTETERLEKILANVMDFTRPSPFLLRFNNINEIIDDTASLLSNLFQERRVELSL
                     QLQEVPLIKSDFNQMKQVMLNLLQNALDATPPYGIVEVKTYSDDKKVYIEVSDTGSGI
                     VEEDIYRIFEPFYTTKTTGVGLGLAIVKKIISDHNGDIKVINKERGAVFTISIPLP"
     gene            8954..9313
                     /locus_tag="PLJ75_06600"
     CDS             8954..9313
                     /locus_tag="PLJ75_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04923.1"
                     /translation="MKKILIVEDEQHQRELYAMELQEEGYEVDQATNGKEAVDKVKAN
                     KYDCVILDIRMPEMDGIEALGKILSRDKKVPIIIYTAYSNYKSNFMTWTADAYITKSS
                     NLKELKDKIKELVSTRS"
     gene            9313..10533
                     /gene="glgC"
                     /locus_tag="PLJ75_06605"
     CDS             9313..10533
                     /gene="glgC"
                     /locus_tag="PLJ75_06605"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPD04924.1"
                     /translation="MDLQNVLKSTLVFILAGGQGERLYPLTKDRSKPSVHFAGIYRII
                     DFSLSNCLNSNLTRIIVLTQYKSLSLDRHIKTAWNLYFRESGNFIDVVPPQQRFDENW
                     YMGTADAIFQNIYIIQHEKPTYVIILSGDHIYKMDYQKMLKYHIEKNADMTIAACEMP
                     INEAHRFGVLETDSNLKVVGFEEKPQYPKPVPGTENAAFVSMGIYIFTTDKLVRYVSD
                     DHKSDSSKDFGKDIIPKIYETENVFAYNYVANEGERAYWRDIGTIASYYEAHRDIISI
                     SPILNLYDKEWPIRTYMEQWPPAKFIHNEEGRRGIAINSLIANGSIISGGYVEQSIIS
                     PGVKVNSFSEVRGSIIMHGSTIGRYAKIQNAIIDKDNHIPEHMQIGFDLEEDKKHFMV
                     SPEGIVIIPKSYVQ"
     gene            10653..12710
                     /locus_tag="PLJ75_06610"
     CDS             10653..12710
                     /locus_tag="PLJ75_06610"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPD04925.1"
                     /translation="MSCNFIFCLHNHQPVGNFDHVFQWAYNDCYRKTLDILYKYPEFK
                     FAIHNSGPLLEWIEDHDASYCDTLALMVERGQVEVIGGGFYEPIFSIISDNDIHGQLS
                     LMQSFCKKRFGVEPAGFWTAERVWDPEIPSLVYDYKLKYTLLDDIHFRYAGIPESGLY
                     GYYITERLLKPLYVFPIDKFLRYSIPFKLPHETIDYFKRQCDKYGHCAFVYGDDGEKF
                     GVWPQTFQWVFEEKWLQNFIEAILKEDFISLIHPGEYIQYNPPIDRVYLTQGSYYEMS
                     EWALPADSGEQLVQLHNEIKNAGREAEFYPFLKGGVWNNFLNKYPESNAINKRTLYLS
                     REINAIEIKTGKDMSQAKTELYRAQCNCAYWHGLFGGIYLTHLRSALYKHIAVCEDMI
                     CAEKKLNTPEIIQGDFRNEGHEQIFLRNQTCSIIVNPAFGATISEFTNRKKRTNVFDT
                     IARRKEAYHSILHNIKDDDKNATNVQSIHEMVTVKEKGLQKYLVYDSSRRYSCKDLLF
                     TSLPQPDDLMFNRIDYIDCSLHPFSCTMNDTTIQCDSSSDVLPAISKTFSIHATKPIL
                     TVHYDIEGYNGWFGLEFTINMLVPHSKECYYAIEGVAAENAYLDSMGTSSGITQVTMV
                     DTTRDVSFSLQTSKPFILVRYPVYTVSQSDSGFEKNYQGSCMVLCFETNNSLHLDCEL
                     TVQ"
     gene            complement(12781..13149)
                     /locus_tag="PLJ75_06615"
     CDS             complement(12781..13149)
                     /locus_tag="PLJ75_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HPD04926.1"
                     /translation="MDDQEKLPERILVADEVASILTGDNIVKALANCKVDTPEKEHAI
                     EFVKAHYNFIQEIVQNDIQRKIIRSDFEIKDLVAHVNALMQSKDEYLFTMIVMHSPKH
                     YQQIQKSLLQEINKKEKEKS"
     gene            complement(13308..14528)
                     /locus_tag="PLJ75_06620"
     CDS             complement(13308..14528)
                     /locus_tag="PLJ75_06620"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HPD04927.1"
                     /translation="MKRYFFWTSLCIIVALVACGGAQKQQKPSKTACEMCGEADGFAA
                     IFDNDTALARDRAIDDAMNKLVKMKLGTSIEGRTVVEDYALVESIVEAKSTGMVRNWK
                     VIKEGAQEGAYTVRIYGEVYPQAVNDTIEATLKNYGRPKFMILINETFEGQKHEPGFT
                     VTELTMMEIMQNAGFEFVDAATVQQLIKKDKAKMMQAMIGRVGGAVQELLLDDVGAEC
                     IIVGDVTTTDQSNVLQQYSANMKSKQAILNLKAIDVYTGSILASTSTNMPGAHINAEA
                     ASKKAIQSALVKILGSTDEETGKFKSGPFMNQITKKFLLAATHRMITLNIVGLDYNDL
                     TKFRDILQNRIRGIQKVYVRGQAGKQSKIDVEFAGKTTDLADELVGKASSIGFKIEVK
                     ETYPNKIMLIAQKL"
     gene            14782..16899
                     /locus_tag="PLJ75_06625"
     CDS             14782..16899
                     /locus_tag="PLJ75_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPD04928.1"
                     /translation="MSLSSLYNLPVDQITRESYFNVGGMHGFMRWGVYIFLIIAAIFI
                     LYSLIQKALIWRRGKPELRTDFPEKRIIALIKYVFLQKKVLREKYAGIMHACLFYGFI
                     GLFIVTFIILIQEDVTELFFHFKFLVGDAYLLWSFAGDIFGVAVIVGLVMAFWRRYKV
                     KPSRLDTKPIDTFALILIAFIILTGFLNESLRIAITGFPKFEVWSPVGYSLAHLFGWL
                     SIPTLEITHYINWWLHMIGAFSFIGLIATDKLGHILTTMLNVYFQNLDNESPKTKFAM
                     PVISPKEFETAETFGVGMVEHFTWKQLMDGDACTRCGRCQDNCPAYLTEKPLSPKKII
                     NDVKDAMDDRIPKLMTADDPSTVESKALIGEYVLDDEIWSCTNCAACMENCPVQIEHV
                     PKIVDMRRYQVLMEGKMATELQTSFGNMENNSNPYGFAFAARADWVTEDLGVKTLAED
                     PNVDFVYFVGCAASYDKRNQKVALALCKILQAAGYKVGILGPEEACCGDSAMRAGNEY
                     LFHALATQNLETFKNYGIKKIITTCPHGYNTIKKEYPKFAKVGVDSSGNPLEANYEVY
                     HHTEVIMNLIKSGKIRLTKPLNETITYHDSCFLGRYNTIYDQPRNILKAIPGTNFVEM
                     SRNHERSFCCGAGGARMWIEEHLGTRINQFRTKDAQATGATRIGTACPFCLTMLSDGA
                     NELDIQNLQTFDLAEYVWEAMEK"
     gene            complement(16976..17500)
                     /locus_tag="PLJ75_06630"
     CDS             complement(16976..17500)
                     /locus_tag="PLJ75_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HPD04929.1"
                     /translation="MPLYEINDRKPVIGEGTWIAPSAQIIGDVRIGSRCYIGFGAIIR
                     GDYGTIIIGDESAIEELCMIHARPMQLVTIGRRVTVGHMVMIHGATIMDNAVIGMHAT
                     VSDNAEVGEWAIVAEHALVVNKQRIPAYKIYGGVPAKEIGDVQQKHKDIWDAGKQVYV
                     DLTYQYPQSFHKID"
     gene            complement(17516..18457)
                     /locus_tag="PLJ75_06635"
     CDS             complement(17516..18457)
                     /locus_tag="PLJ75_06635"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD04930.1"
                     /translation="MDNTITLYSKVKIILKRYFEALNMEQRVVVPQYDADYKPKWITI
                     ILLFIATFITTSIAGSNAGGSILEIIINGLPFSLSLLAILFAHEMGHYITARYFGVIA
                     TPPYFIPFPSLIGTMGAIIRIKSPIPDKRVLLYIGAMGPIAGFIISCILVIVGLMQSS
                     VQPLPKPSGDSFVPIFGDSLLFVFLVKMILGNIPQGYDVYFSPLAWAGWIGFLVTSLN
                     LMPIGQLDGSHVLYALFGEKQRYAGWLFFGIICILTFLWVGWVVWIILTVSLLMIGHP
                     YIPDGKPLTAAEKIMGYLCIIIFIFTFIPVPVVISGQ"
     gene            complement(18462..20600)
                     /locus_tag="PLJ75_06640"
     CDS             complement(18462..20600)
                     /locus_tag="PLJ75_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD04931.1"
                     /translation="MNPLEKLINPSSIAVVGASSNPLKMATLHLLSIMKDGYKGRIYV
                     IHPKEESVFGLKTYKSPLDLPEAVDCAFFVIPAQNIPDTLKDFGTIGTKRAIIISAGF
                     KEIGQEGKLLEDKLKSTAGINGIRFLGPNCMGIVNTEISLNTTVMEYIDRPGSLGFAT
                     QSGTYITQTLRYLKNHGIRLSKAVSVGNEADISIIDVLEYLANDEQTKAIALYIEGIR
                     DIKRFLNVAITITSKKPIIAQYVGGSQAGARAGSSHTGAMAGPDYLYEGLFKQAGIIR
                     VHSIEELYFHGYMLATQPGLKGNRVGIITNSGGPATSMANSCEKEGFIVPTFSEGLQS
                     NIKPLIPPHAQAGNPVDITFNLDLDVLTIKIPELAIQSGEVDALCIHGAYRKGFVEAV
                     YPHIREILNIQKPEDIINMFPSDLHTPGTIPKKYGIPLVMSSFYDYQDDFTKAMQDHD
                     VLVFDAPEKAAKALAALLRYKEIINRLPYEPITLPETNSEAKAIIQGAKKNNQKALDE
                     YMAKRLLSLYGIPIPDERLIENEKQLDGIVTSMSYPIVMKACHPNIMHKTEKGLVMLP
                     IKDSIEAKQAFQTIQSRAGYAIPVLAYPMVEGKREFMVGAVNNKGFAQSVLFGLGGIY
                     TEVVQDATFRIAPLAKRDAYEMFNDIKAKTLLGKFRNMDEVDRDTLAQLIVTVSMLPV
                     LHPEIAEIDCNPVIISGNKPVVVDALIIIE"
BASE COUNT         6672 a         3809 c         3603 g         6502 t
ORIGIN      
        1 atgaatatgc tggcagagca ggatcctcat cttgatgtgc aggaaccagt aacattacgc
       61 gttactatgc aggagattgc aaactgggca ggtatgcaga taaatgatgt tcaaaagcta
      121 ttagcacatt taaataaact gggaaaaatt gagctgttta acgatcgtgt tatagttcgc
      181 aatatcagag acttccagcg gatggtaaat tctagacgta aaagcatgta tgcgtaggct
      241 cccgtagctc aggtggatag agcagtagtt tcctaaacta tgtgccagag gttcgagtcc
      301 tctcgggagc agagatgaaa ataacatata cattagcggg cttatcccgc ttcttttttt
      361 aggggtattc actatggaat cacggttaca acaacttctt gacagatatc atgaaattga
      421 acaggcaatg gcagacccaa aaaccgtaaa tgaccagaac ctgtttaaat ctttaacaaa
      481 agagcattca aaattatttc caatagtaca gaaatataat gaatatacaa aaattaaaaa
      541 agaaactgaa gaagttacca atgcgttgaa agaagaaaat gaaccggata tggttgccct
      601 tttagaagat gagaagatac aattagaatc aaaaatgaat gaaatagaag aacaaataac
      661 acagttactc cttccacagg atccacatga agggaaagat attatcattg agatacgtgc
      721 tggcacggga ggtgatgaag cttctctatt tgtggctgat ttatacagga tgtatatccg
      781 gtatgcagat cttaaaggct ggaaggtaga gtttattgat gccaatcaaa ctgaacttgg
      841 tggttttaaa gaaataatat tttcaattag tggtgatgat gcatatagct gtttaaaatt
      901 tgagtcaggt gttcaccgag ttcaaagaat acctgttact gaatcaggag ggagaatcca
      961 cacatctgca gtaactgttg cagtacttcc cgaagcggaa gaaaatgata tccagataaa
     1021 tcctgatgat atccgcattg atgtctaccg ttcatcaggg catgggggac aatcggttaa
     1081 tactaccgac tctgctgtac gtataacaca tattccaact ggcattgtag ttacctgtca
     1141 ggatgaaaaa tctcaacata aaaacagagc aaaagctcta agagtactgc gtgcacgtct
     1201 gtttgaaaaa ttagaacgtg aacgcatgga aaaagaaaca gctttacgcc gttcgcagat
     1261 tggaagcggc gatagaagtg agcgtatacg aacatacaat ttcccccaat caagggttac
     1321 cgatcatcgt ataaacttaa ctatttatga tttagaatca gtacttgatg gtgaactgga
     1381 taaaatcatc atccctctca gagaatatga tatggaacag atgctaaaag cttcccatgt
     1441 atatcatgca taacaatgct actatttttg atatcctgtc acaagctgct cacaggttag
     1501 cttcaatttc tgcaacacca gccctggatg ctgaaatatt gatggctcat acactcaata
     1561 ttgaacgtca tactctccca ttgcattatt ctactcacat cagcaaaata atatataaaa
     1621 aatttgaaaa tttactatta cgcagattac aaagcgaacc aattgcttat attataggtt
     1681 ataaagaatt ttattcactt ccttttcatg taaaccaaca tgtcctgata cctcgtccag
     1741 aaactgaact cattgttgat tttgcccttt attatgcaca tcacaatgca acaatacttg
     1801 atattggaac aggtagtggc gcaattgcta tcgcaattaa atataaccta acagattgtt
     1861 ctgtctgggc aacagatata tcagcagatg ccctgcgtat tgcaaaaaaa aatgctgcca
     1921 ctttattagg gatgaacaaa attaaatttc ttcaaagcga tttgtttaca tcgttacctc
     1981 acgtacactt tgatgtgatt gttagtaatc caccatacat caatcctgaa atttatacta
     2041 ccttgcaaaa agatattttc tttgaaccaa aagaagcatt agttgcaccc gatcatggtt
     2101 ttggcatcat caaagcaata atcaatgatg cttataacta tctaacaaaa gatggagtat
     2161 tgattgtaga gataagtgat gatttagccc atcagatagt caactatact cagtcattgc
     2221 cctatacaat ttccattgca aaagattatt cgggtatgca acgtgttgca attttgcaca
     2281 aaactataaa aaatataagc aattaagaaa taatgataaa tttaaaataa ttttattcga
     2341 tactgtaata taactgatgt atcagcccaa taagcatggt agtggagtaa gcagaccgct
     2401 aaatgagcat tccatctgaa aggggaatca ttgtgcatga tcaatggtaa agaggaatta
     2461 ctgcatgata tcagagttgc caactttata gaagcatatc gttttagttg cataagcaaa
     2521 cgtgataagt ggtatagctt ttcaaatatt gaatgggatc caatattaag tgaaattaaa
     2581 attgaagcta tcatcaactt taatgattct tcatacacca tcaatcaaat tattagacag
     2641 gacacaatcc caaaaaaatc tattggtaat caacggttta aatacacaat acttgaacca
     2701 ttaaaagaaa gtaaaatatt ctttaaagaa agtgatatca caggcaccat acttattcag
     2761 aactatttcc cttattacga tatcattcat cattcatcga tatcaccgaa taatgttgaa
     2821 aatatgttca atttttactc tgaccggtgc aaggctaaag tttcagttga attgcacaaa
     2881 aaaaataata ctactcataa atattttgac tgtgtaggat ttagagaaca ctcatggggg
     2941 ttatatcctt ctcatcatat tagttgtgat agcagagtag ttgtgcattt cagagatcaa
     3001 acaatttcgt ttagatatat agaatataat ggcatgagct attcatatgg ttgtatttca
     3061 aaaaaaagtg gcaatcttgc attggtcctt gttgagttag aatttctttc aataaaaaat
     3121 tcttcttttg agtcatcaga atttacctac aaagatgctc aggatgatat agatctgata
     3181 gtttcaaaac ctataaaacc acaccatatt gaggtatcaa aaaaactaaa aaaatcccat
     3241 ctccatattg ttagtttttc cgattttacc gttataggaa caaacaaaaa aggatatggt
     3301 attgaagaac atcttatttc atatgaacga ttaaaaaatg tgctataatg tgaatttatg
     3361 acaatcacat tagaacagat cagaaagctc agtatctttt taattatcct atttttgtta
     3421 ttactctata acatatattc aaaactattc ttcgactggt atggtattga cattattgtc
     3481 cgttcatatt cttttttatt ttcatttttt tgtatattta actacactca aattgatatt
     3541 aaacagtaca aaaccatata ccaaaaacga tatcgttctt atgcaaactt tttattattt
     3601 tttgaaactc gccttattcc atttttattt atatactttg tatccactct tcatacttta
     3661 attgacactt taaataaaag caactggcca tatgcaggat actttggcat tttagatgga
     3721 cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacaacca gaaattgcaa
     3841 tcggctcaac atttagcaca gcttgctgca cgttttgccg atgggaagga aaacataatc
     3901 attgatttat ttgaaaaggg aaatatgtcg tttaaaatct gggcaatgaa tgttatgcag
     3961 catcataaca aaatgatcta tataccacct ttattgcctc attgtaccga tatcaatgag
     4021 actatgtcat caacagcata tagcaccata acaaaaatca ccggtaaaaa tttttcatca
     4081 cagctcaatc ttccctataa taatccaaaa gtattaagag caatcaaaga tttttatttt
     4141 caaaagaaca ccatacagta atggggcaat ggctgcattg aggttttctt gccatgcagg
     4201 tattcctgcc atggtaaata aatagaaaat gggcagtaat ccattctgat tcatcaatgc
     4261 ttttcattaa catggtttca atttcataaa ctttttttga actgcaataa ccaatcctgt
     4321 gagcaaccct ggctacatgc gtatccaccg gtattgcagg tataccaaat cctgtagcta
     4381 aaatcacatt agctgatttc cttccaatgc ctggcaaact catgagctgc tccctatctg
     4441 ctggcagatt tccattaaac ttttcaataa cctgttgcga caatgcaata atatgttttg
     4501 ccttggtatg ataaaatcct gttggtttta ttatgtgttc aacttcatgg acatttgcag
     4561 cagccagggt agcaaagtca ccatactttt tgaacaatat tggtgttacc ctgttcacct
     4621 gattgtcggt tgtctgtgct gataaaacaa cagcaataca gagctggtat acattggcat
     4681 attttaatgg cggagcaaca tcagggtatt ctttttttaa ccgtgtgatt attttttttg
     4741 aagtgcctct atcaatcatg aactatcgcc actattattt tttgtgcctc tttttttctt
     4801 tcctgtaacg ctgcatcatc cgctaaatca tcattgttga tgtaataccc acaaataaat
     4861 ggctggttta atttcattgc cacaatggta tgcagatcat ccagtgcttt ttttttgttg
     4921 ttcaaagcca ggtaacattg tgccctgtaa aaatatgcct caaagtgctg tgggttttgt
     4981 tcgatagctc ctgtacaata atctattgct ttgacagtat cattcgtata atagcaagcc
     5041 agtccagcac caaacaggat atcataatca atatagtttt tttgtattag acgggtaaaa
     5101 tccagcatag cccactcata gtgccgttta tttatgtgtt cccagccact tattattgtt
     5161 tgattgatat atggatcatt aaaacgccag tgatgttggt gtttaacatt ctttgttgta
     5221 gtacattgta aatgtatcaa taccaaacca gcgcaaaaaa ttgttaaggt tttactgctc
     5281 atgggatttt tgccttacca ttgtctgtaa tagatttttt accttttgta atgcctttgc
     5341 cctatggctt atagtatttt ttactgacag aggaagctgt gccatggtct tatcaaaata
     5401 aggaagataa aatatggggt catacccaaa cccgttatca ccagcaggcc gtgtggcgat
     5461 aaaaccatgg catataccct cttcgatata ttctttacca tcagggaata ccagtgccat
     5521 cacacagata aagcgggctg tccgcttatc ccacggaaca ttgatcatct ttgaaagtac
     5581 aagcctgcaa cgatcatcat ccgttgcaca attgccatac cgggcagaat atacgcctgg
     5641 ctcgccattc aatgcatcaa tcacaagccc tgaatcatca gcaagtgctg gcagaccact
     5701 aacagtacaa acatagcggg cttttattaa tgcattttcg gtaaatgttg ctcctgtttc
     5761 ttcaatttct ccaatttcag gatatgcatc aagagaagta aaaataaccg aaggaatatc
     5821 aaaagcaaca atttctttca ttttcccatt gtttcttgta gcaacaacaa catccatggt
     5881 gccccttcac ttaatggtta tatgataaac atagaataat aaataaatgt tgcatgtatt
     5941 ggtaaagcct gaacaaagtg ttattggcca tttgattgga gtactattat taaaaataat
     6001 tgcacggtat gcatttaata atcaatgcaa tactattata tgtttcatgt atatattttc
     6061 aatatttatt gttgcatttt cattgaaaaa attgttacta tgatagtata ttgttgatag
     6121 ctatggaaga aaaaaagcag attaatatat tacgtattgg tgttgcaaat cttactgaac
     6181 tggaagagtt agttaaagct ttccgattaa tgaatcagta ttccaaacga agaagatttg
     6241 tagtttcacg cgaggattta aaagatatat atggcaataa aattgttgaa aaagctcatg
     6301 atattaatat atccgttgtt aaattgctac agcgaaattt taaacctgat accgttttta
     6361 aaatattcag ctctgatgaa gggatagcaa tagttaccta ttttgaatca atgacaggca
     6421 aggatttttc tatgcaactt attgaaacaa tcggtaacat cggaggtggt atatacaaac
     6481 cttttattga tgtagtgcca aattttcatg agctttttaa attgtttgaa aagggtcttt
     6541 cgccaaagct ggttgttgtt ggttatattc cggtaaaaaa cgttgcccag gaagtctcaa
     6601 tgtataatga aataaaaaaa tttgaccctt atattagatt gctcgacatt accaatgatg
     6661 atttaaaacc aaaatccttc ttaaaaggag cagtcttatt tcattttgac aataccctga
     6721 aggtgtggca ccgactattg acaaagatta tcaatgaata tacaaaacca tacttcatgg
     6781 aagagatttc aaagtaatga tacgctgtga tatagtgcat ggatgaaaga aaactacaac
     6841 tttttgctcc tgtcatattg atccttacta ttataacaag cattagtatt acaataatgc
     6901 tttatttaca gaaaacaaac attatcatga ttgtactttc ttgtattctc tctgctactt
     6961 tgataacatt attgattatc ttcaggctga tccataaagt ttttttcact aataacatta
     7021 gaaagatatt atcagttgtt gataatttta aaaaaggtat ttttgtccaa ccttcaaatg
     7081 aaattaaagg taacgattta ctttcaaaaa tgtatggtga attactcatt gcctctcgcc
     7141 atcttgataa tataatcaaa tcgcaaaaaa acgagatcga taaatttcgc gagctctata
     7201 acaatatgat gttttcgatg aacgcttata tcatggcact tgactttaac aataaaataa
     7261 tttttactaa cgagttattt ttaaataaat ttcaactatc cccttctgaa gtaaatggaa
     7321 aaaatttatc agatattttt tattttttaa caggtacggt attgcaggca attgaagaaa
     7381 ttaaatcgac acatgaatca ataattttag aaaaagctca tctcatatca cataaccgta
     7441 ttagtatcat cgctgatatt aaattatcct ttatgatttc caacaatgaa ccgcaaatta
     7501 taatggttat ggatgatgtc accagcaaaa caaagcgcga ctaccagata tcactgatta
     7561 cacggttgtc tgaaacaatt cagaaagatg atgctcttga ccatattttg cgtactattt
     7621 tgactgcggt aacatccggc acaggattag gatttaaccg tgcaatgctc tttttgcggg
     7681 atgataaaaa caatatttta tatggcagga tgtcagttgg gcctgatacc cttgacgaag
     7741 ctttttctat atggagtagt ttaaatcctc tgaaaggaaa agaaatttta agtcgtggaa
     7801 taaatggaga tgatgtccat ggtagattac tgaaagaaaa ggttgaaagt acctcttttg
     7861 atttaaacga agatactatt tttactcaaa cacttcaatc aggtgaaaca attcatatca
     7921 ctgatgcttt acacgatcaa cgagtcaatg atgaaattaa aaattttatt gatgttaacg
     7981 agtttgttat cgtacctctt atagcactta ataaaattat tggtatatta attgcagata
     8041 ataaatttaa tcaaatgcca ataacccagg atagtgttga tttattaaca gtatttgctt
     8101 atcaggctgc actttcaatt gaaacctata acaacctgga attattaaaa aaggaaatgg
     8161 aaaaaatcag acatcggcag gaagcaattg ttgaatctga aaaattagca gcaatagggc
     8221 gtatagcttc tcatattgcg catgagatca ggaatccact ggtaaccgtt gggggatatt
     8281 cccgaagaat attacaaact gctaatgcac agcagataga tatacaaaaa ataaaaaaag
     8341 cagctcaaat tataatgacc gaaaccgaac gtctggaaaa gatccttgct aatgttatgg
     8401 actttacccg accatcacct tttttgctgc gctttaacaa tataaacgaa atcattgatg
     8461 atactgcatc attactatca aatctttttc aggaaaggcg tgtagaacta tcgctacaat
     8521 tgcaagaggt accgcttatt aaatctgatt ttaatcagat gaaacaggtt atgcttaatc
     8581 tgttacagaa tgctctggat gcaactccac cgtatgggat agttgaggta aaaacatatt
     8641 ctgacgataa aaaggtttat atagaagtaa gcgatacggg gtcaggaatt gtagaagaag
     8701 atatatacag aatttttgag ccattttata ccacaaaaac aacaggcgtt ggtttagggc
     8761 ttgcaatagt taaaaaaatt atatccgacc ataatggtga tatcaaagtt ataaataaag
     8821 aacgtggagc tgtttttacc atttcgatac cattaccgta aatattattt ttttactttt
     8881 aaaaaaaatg tctttgattt tttttgtagt cactatacat tgattattag aaaagtataa
     8941 ttgggaggtt gttatgaaaa aaattcttat tgtagaagat gaacagcatc agagggaatt
     9001 atatgcaatg gaacttcaag aagagggata tgaagtagat caagctacca atggcaagga
     9061 agccgttgat aaggttaagg caaataaata tgattgtgtg atacttgata tacgaatgcc
     9121 cgagatggat ggcatagaag cactgggtaa aatcttatca cgcgataaaa aagtacctat
     9181 catcatctat accgcctatt ccaattataa aagtaacttt atgacatgga ccgctgatgc
     9241 atatattacc aagtcatcaa atttaaaaga actgaaagat aagataaaag aattggtatc
     9301 aacacgaagc taatggatct gcagaacgta ttaaaaagta cacttgtttt tatacttgca
     9361 ggcgggcagg gtgaacggct ttatcccttg acaaaagata gatcaaagcc atcagtacat
     9421 tttgcgggga tatataggat tatagatttc tcattaagta actgtcttaa ttcaaattta
     9481 acccgtatta ttgttcttac ccagtataaa tcgctttctc tggacaggca tataaagaca
     9541 gcatggaatc tttatttccg agaatcagga aatttcattg atgttgttcc accacaacaa
     9601 cgatttgatg aaaactggta tatgggtact gctgatgcaa tctttcaaaa catatatatt
     9661 atccagcatg aaaaacctac atacgttatc atactatcag gtgatcatat ttataaaatg
     9721 gattaccaaa aaatgcttaa ataccatatt gaaaaaaatg ccgatatgac aattgctgcc
     9781 tgcgaaatgc caattaatga agcacatcgt tttggtgttc ttgaaaccga tagtaactta
     9841 aaagtagttg gctttgaaga aaaacctcaa tatccaaaac cggttccagg aacagaaaat
     9901 gctgcatttg tatcaatggg catttatatt tttaccaccg ataaattagt acgatatgtt
     9961 tctgatgatc ataaatcaga tagctctaaa gattttggca aggatattat tcctaaaatc
    10021 tatgaaaccg aaaatgtttt tgcatacaat tatgttgcta atgaaggtga gcgtgcatac
    10081 tggcgtgata taggaactat tgcttcatat tatgaggctc atagagatat catatccata
    10141 agcccgatac tgaaccttta cgataaggaa tggcccataa ggacttatat ggaacagtgg
    10201 cctccagcta aatttattca caatgaagaa gggagacgcg ggattgccat taattccctc
    10261 attgcaaatg gctctatcat aagcggaggc tatgttgagc aatcaattat atcacctggt
    10321 gtaaaggtta acagcttttc tgaagtacgt ggaagtatta ttatgcatgg gtcaactata
    10381 ggccggtatg caaaaattca aaatgcaatt atcgataaag ataatcatat cccagaacat
    10441 atgcagatag gatttgatct tgaagaagat aaaaaacatt ttatggtttc acctgaaggg
    10501 attgtcatca tacctaaatc atatgttcaa taaatttata gtaaatccca tcccattgcg
    10561 atacattgat gcacaatacc atgtatgagg gatttactat tgctaatact tcttcagaat
    10621 gatacagtac atctctattg acggaggtag gtatgagctg taattttatc ttctgtctcc
    10681 ataaccatca gccagttggc aattttgatc atgtattcca gtgggcatac aatgactgtt
    10741 accggaagac acttgatatt ttgtacaaat atcctgagtt taaatttgca attcacaact
    10801 ccggtcctct tttagaatgg atagaagatc atgatgcatc atattgtgat acattagcgt
    10861 taatggttga gcgtggtcag gtggaagtaa taggcggtgg attttatgaa cctatatttt
    10921 caattattag tgacaatgat attcatggac aactatcgct tatgcaaagt ttttgtaaaa
    10981 agcgttttgg tgttgaacct gctggtttct ggacagccga gcgggtttgg gaccccgaaa
    11041 tacccagtct ggtctacgac tataaactta aatatacact gcttgatgat atccatttcc
    11101 gctatgctgg aatcccggaa agcggattat acggctatta tatcaccgaa cgtctgctaa
    11161 aaccactata cgtttttcct attgataaat ttttgcgata ttccatacct tttaaacttc
    11221 cccatgaaac tattgactat ttcaagcgtc agtgtgataa atatgggcac tgcgcttttg
    11281 tttacggcga tgatggtgaa aaatttggtg tttggccaca aacattccaa tgggtatttg
    11341 aagaaaaatg gctgcaaaac tttatcgaag ctattttaaa agaggatttt atatcactca
    11401 ttcatcccgg agaatatata cagtacaatc cgcccattga cagggtttat cttactcagg
    11461 gcagctacta tgaaatgtcc gaatgggcgt tgcctgcgga tagtggtgag cagcttgtgc
    11521 aattgcacaa tgagataaaa aatgcaggaa gagaagccga gttttatcct tttttgaagg
    11581 gcggtgtatg gaataatttt ttaaacaaat accccgagtc caatgcaatc aacaagcgca
    11641 ccctctattt atctcgtgaa atcaatgcta ttgaaataaa aaccggtaaa gatatgtcac
    11701 aggcaaaaac agaactgtac cgggcacagt gcaactgtgc ttactggcat gggctttttg
    11761 gaggtatcta tcttacgcat ttgcggagtg cgctttacaa acatattgct gtatgtgagg
    11821 atatgatctg tgcggaaaaa aaactaaaca cgccagaaat aatacaaggt gattttagaa
    11881 atgaggggca cgaacagatt tttttacgca atcaaacatg tagcattatt gtcaatccag
    11941 cttttggtgc tactatttcc gaatttacca atcgtaaaaa acgcaccaat gtttttgata
    12001 cgatagcgcg ccgtaaagaa gcataccata gcatattaca caatattaag gatgatgaca
    12061 aaaatgccac caatgtacaa tctattcatg agatggttac agtaaaagaa aaaggattgc
    12121 agaagtatct tgtgtacgat agttctcgaa gatattcctg taaggattta ttatttacca
    12181 gcttgcccca acctgatgac cttatgttta acagaataga ttacattgac tgctcgttgc
    12241 atccattttc atgtaccatg aatgatacta ctatccagtg cgatagctca tcggatgtat
    12301 taccagcaat aagcaaaact ttttcaattc atgcaactaa acctatttta acagtacact
    12361 atgacataga aggctataat ggatggttcg gattagagtt tactataaat atgcttgtcc
    12421 cccattcgaa agaatgttac tatgctatag aaggtgtagc agcagaaaat gcctatcttg
    12481 acagtatggg tacttccagc ggcataactc aggttactat ggttgataca actcgtgatg
    12541 tatcattttc attgcaaaca agcaaaccgt ttatacttgt gcgctaccct gtttacactg
    12601 tttcacaatc tgactcagga tttgagaaaa attatcaggg gagctgcatg gttctttgct
    12661 ttgaaacaaa taactcactc catttagatt gtgaacttac tgtacaatga aattgaatcg
    12721 caatgtgatt atggatattt ttatttcaca cttcaggaaa atcaatgagt aaagtactta
    12781 tcatgatttc tctttttctt ttttatttat ttcctgcaac aatgattttt ggatctgctg
    12841 gtagtgtttt ggagaatgca tcactatcat ggtaaacaaa tattcatctt tactctgcat
    12901 gagtgcatta acgtgtgcca ctaaatcctt tatctcaaaa tccgaacgta ttatttttct
    12961 ttgtatatca ttctgcacta tctcctgaat aaaattataa tgggctttta caaattcaat
    13021 ggcatgttct ttctcaggag tatctacttt acaattggca agcgctttaa caatattatc
    13081 gccggtcaat attgatgcaa cctcatcggc tactaatatt ctctcaggca gcttttcttg
    13141 atcatccatt ataaactccc gctacaaaaa cttttcaaat ttaatgtatc caaccatgca
    13201 acatgtgtca atcaaaataa atgccagaga aactattctc tggcattttt gtgtaacaaa
    13261 gctattgtta ctataagaaa gagtaataat gtctatgaaa aaccttctta caatttctgg
    13321 gctatcaaca taattttatt gggatatgtt tctttaacct caattttaaa cccaatgcta
    13381 ctcgctttac caaccaattc atcagctaaa tccgttgttt tgccagcaaa ctcaacatca
    13441 atctttgact gttttccagc ctgcccgcgg acatatacct tttgaatgcc tcgtatccta
    13501 ttctgtaata tatcacggaa tttagttaaa tcgttataat caagccctac aatatttaat
    13561 gtaatcatac gatgtgttgc agctaacaaa aattttttag taatctgatt cataaatgga
    13621 cctgatttaa acttacctgt ttcttcatca gtactgccta aaatctttac aagggcactc
    13681 tgaatagctt tctttgatgc tgcttcagca ttgatatgag cacctggcat gttggtactg
    13741 gttgaagcta agatgctacc ggtatagaca tcgatagctt tcaggtttaa gattgcctgt
    13801 ttagatttca tatttgctga atactgctgt aaaacatttg actggtctgt tgttgtaaca
    13861 tcacctacta tgatacattc agctccaaca tcatcaagca aaagctcctg tacagcccca
    13921 ccaacccgtc ctatcatcgc ctgcatcatc tttgctttat ctttttttat taactgctgg
    13981 acagttgctg catccacaaa ttcaaacccg gcattttgca taatctccat catggtaagc
    14041 tctgtaacag tgaagcctgg ttcatgcttt tgaccttcaa aggtttcatt gatgagtatc
    14101 atgaacttag gacgcccata atttttcaat gttgcctcta tagtatcatt aacagcctgt
    14161 ggatatacct caccataaat tctgactgta tatgcacctt cctgtgcacc ttctttaata
    14221 actttccagt ttcttaccat acctgttgat tttgcctcaa caatggattc tactagcgca
    14281 taatcttcaa cgactgtacg tccttctatg gaagtaccca gcttcatctt caccaatttg
    14341 ttcatagcat catcaatggc tctatcacgt gcaagtgctg tatcattatc aaatatggca
    14401 gcaaacccat cagcttcacc acacatctca catgctgttt ttgatggttt ttgttgcttc
    14461 tgagcacctc cgcatgccac taatgcaaca atgatacata aacttgtcca gaaaaaatat
    14521 cgtttcataa ttggacctca taattattga atatttattg tatatgaatg atactatagt
    14581 accatattaa agttacgtca aaccattttt ataaattatc aattaccaca ttctaaaatt
    14641 accaacatgt tcataggtta ctattcaatc ataatattct cactattcta tttaaaatag
    14701 tgttgacaag gacttccagt attaaagtat tgttaactaa agttagatgc aattattttt
    14761 tatggataag gagggttgta catgagcctt tcaagtttat ataatctgcc tgtcgaccag
    14821 ataaccagag aatcgtattt taatgttggc ggcatgcatg gtttcatgcg atggggtgta
    14881 tatatctttt taataatcgc tgccattttt attctttaca gcctgatcca aaaggcacta
    14941 atctggaggc gtggcaaacc agaactcaga acagatttcc cggaaaaaag aattattgcg
    15001 cttattaaat atgtattttt acaaaaaaaa gttttacgcg aaaaatatgc agggatcatg
    15061 cacgcctgcc tgttctatgg ttttatagga ctattcattg taacatttat aatcctgatt
    15121 caagaggacg ttactgaact attcttccat tttaaatttt tggtagggga tgcatacctg
    15181 ttatggtcat ttgcaggcga tatttttggc gttgcggtta ttgtagggct tgttatggca
    15241 ttctggaggc gttataaagt aaagccaagc aggctggata caaaaccaat tgataccttt
    15301 gcactgatat taatagcttt tattatcctt accggatttt taaacgaatc gctcagaata
    15361 gccattacgg gattcccaaa atttgaggta tggtcaccgg ttggttattc attagcacac
    15421 ctttttggtt ggttaagtat tccaaccctt gagataaccc actacatcaa ctggtggttg
    15481 catatgattg gtgcattcag cttcatagga ctcattgcta ccgataaatt agggcacatc
    15541 cttaccacca tgctcaatgt ctatttccag aatcttgaca atgaaagtcc aaaaacaaaa
    15601 tttgcaatgc ctgtaataag cccaaaagaa tttgaaacag ccgaaacatt tggtgtaggt
    15661 atggttgaac actttacatg gaaacagctt atggatggtg atgcatgtac acgatgtgga
    15721 cgctgtcagg ataattgtcc tgcatacctg actgaaaaac cactatctcc taaaaaaatt
    15781 atcaatgatg tgaaagatgc aatggatgat cgcatcccga aacttatgac cgcagatgat
    15841 ccatcaactg ttgagtcaaa agctttgatt ggtgaatatg ttttagatga tgagatatgg
    15901 tcatgtacca actgtgcagc atgtatggaa aattgccccg ttcagattga gcatgtacca
    15961 aaaattgttg atatgcgccg ttatcaggtc cttatggaag ggaagatggc aacagagctg
    16021 caaacatcat ttggtaacat ggaaaataat tctaatccct atggatttgc ctttgctgct
    16081 cgtgctgatt gggttactga agatttagga gttaaaacat tagccgaaga ccccaatgtt
    16141 gattttgtat attttgttgg ctgtgcagca tcatatgata aacgcaatca gaaagtagct
    16201 ctcgctctat gtaaaatttt acaggcagcc ggatataaag taggtatatt aggtcctgaa
    16261 gaagcatgtt gtggcgattc tgcaatgcgc gcaggcaatg aatatctatt ccatgcactg
    16321 gcaacacaaa accttgaaac attcaaaaat tatggtataa aaaagattat taccacctgc
    16381 cctcatggat ataataccat taaaaaagaa tatccaaaat ttgcaaaagt tggcgttgat
    16441 tccagtggta atcctcttga agcaaactat gaggtttatc atcacactga agttattatg
    16501 aacctcatta aatcaggtaa aataagatta acaaaaccac tgaacgaaac catcacctat
    16561 catgattcat gtttccttgg acggtataat accatctatg atcagccaag aaacatcctc
    16621 aaggcaatac ccggaactaa ctttgtcgaa atgagcagaa atcatgagcg tagtttttgt
    16681 tgcggcgctg gtggtgcacg catgtggatt gaagagcatc tgggaacacg aatcaatcaa
    16741 ttcagaacaa aagatgcaca ggctacggga gcaaccagga taggtactgc atgcccattc
    16801 tgtttaacca tgctttcaga tggtgcaaac gaacttgata ttcagaattt gcagacattt
    16861 gatcttgcag aatatgtctg ggaagcaatg gaaaaataat gtaaaaaaaa tagtaataca
    16921 ttacaattat gaaaaagcat cgccaatttg acgatgcttt tttattttaa tcaatttaat
    16981 ctattttatg aaaagattgt ggatattggt aggtcaaatc gacatacacc tgtttcccgg
    17041 catcccatat atctttatgt ttttgctgta catcaccaat ctctttggct gggacaccac
    17101 catatatttt ataagcaggt attctttgtt tattgacaac cagcgcatgt tcagcaacta
    17161 tcgcccattc tcctacctct gcattatcac ttacggttgc atgcatgcct attactgcat
    17221 tatccatgat agtagcacca tgaatcataa ccatgtggcc aacggttacg cgtcttccta
    17281 ttgtaaccaa ttgcatgggg cgtgcatgaa tcatacacaa ttcttcaata gcagattcat
    17341 cgcctataat aatagtaccg taatctccac ggataattgc tccaaatcca atatagcatc
    17401 ggctaccaat cctgacatca ccaattatct gcgcagatgg agctatccat gttccttcac
    17461 caataacagg cttccgatca ttaatctcat agagtggcat gctaccctcc cttatttatt
    17521 gtcctgatat aactaccggt acaggaataa aagtaaatat aaaaattatt atgcataaat
    17581 aacccattat tttctctgct gctgttaatg gcttcccatc agggatatag ggatgaccta
    17641 tcataagcaa acttacagtt aaaattatcc agactaccca tcccacccat aaaaaagtaa
    17701 gaatacatat aataccaaag aatagccagc cagcatagcg ttgcttttcc ccaaatagtg
    17761 cgtataaaac atgactgccg tccagttgtc caatgggcat aagatttaag cttgttacca
    17821 gaaagcctat ccagccagcc catgccagcg gagaaaaata tacatcataa ccctgtggga
    17881 tattgcccag tatcattttt actaaaaaaa caaacagaag tgaatcaccg aatataggta
    17941 cgaaactatc accagatggt ttcggaagag gttgtacact tgattgcata agtcctacaa
    18001 tcaccagtat acagcttata ataaaccctg ctatgggtcc catggctcca atgtataata
    18061 aaacacgttt atcaggaatg ggcgacttta tgcgtattat ggcacccatg gtacctatca
    18121 atgatggaaa cggtataaaa taggggggtg ttgcaataac tccaaaatag cgcgcagtga
    18181 tataatgccc catttcatgg gcaaaaagga tagcgagcaa tgataatgaa aatggtaaac
    18241 cattgatgat aatttcaagt atggaaccac cggcatttga accagcaatt gatgttgtta
    18301 taaatgtcgc tatgaagagc aaaattatgg taatccattt tggtttataa tcagcatcat
    18361 attgtggtac aactactcgt tgctccatgt taagcgcttc aaaatagcgc ttgagtatta
    18421 tctttacttt tgaatacaat gttatggtat tgtccatggt attattctat tattatcaga
    18481 gcatccacaa ctacaggctt attcccactt ataatcacag ggttacaatc tatctcggct
    18541 atctctggat gtaataccgg tagcatactt actgtaacaa tcaattgtgc cagtgtatcc
    18601 ctatccactt catccatatt tctaaatttt cccaataatg tctttgcttt tatatcatta
    18661 aacatttcat aagcatcgcg ttttgctaaa ggagcaattc tgaacgtagc atcctgtact
    18721 acttcagtat atatgcctcc tagaccaaaa aggactgatt gagcaaaccc tttattatta
    18781 accgcaccaa ccataaattc ccgtttccct tcaaccatgg ggtatgccag caccgggata
    18841 gcataaccag cacgactttg aatggtttga aatgcctgct ttgcttcaat actgtccttt
    18901 ataggtaaca taacaagtcc cttttctgtt ttatgcataa tattggggtg acatgctttc
    18961 atcactatag gatagctcat ggatgtaaca ataccatcta attgcttttc attttcaatc
    19021 agcctttcat cagggattgg aataccatac aaggacaaca atcgtttagc catatattca
    19081 tccaaggctt tctgattatt cttcttagca ccctggataa ttgcttttgc ctcagagttg
    19141 gtttctggta atgtgatagg ttcatacggt aaacggttta taatttcctt atagcgcaat
    19201 aatgcagcta atgccttcgc agccttttca ggtgcgtcaa acacaaggac atcatgatct
    19261 tgcatggctt ttgtaaaatc atcctgatag tcataaaacg atgacataac cagagggatt
    19321 ccatattttt tggggatagt tcctggtgta tgcagatcac tggggaacat attgataata
    19381 tcttcaggtt tctggatatt taaaatctct cgtatatgag ggtaaactgc ttcaacaaat
    19441 ccttttctat aggcaccatg tatgcaaaga gcatcaacct caccactttg tattgccagt
    19501 tccgggattt taatggttaa tacatctaaa tccagattga aggttatatc aacagggtta
    19561 cctgcctgtg catgaggtgg tataagaggt ttaatgttac tctgtaatcc ctcactaaat
    19621 gttggaacaa taaatccttc tttctcacag ctatttgcca ttgatgttgc aggacctcct
    19681 gaattggtaa taatcccgac gcgatttcct tttaaccctg gctgcgttgc cagcatatac
    19741 ccatgaaaat aaagttcttc aatagaatga accctgataa taccagcctg tttaaataat
    19801 ccttcataaa gataatccgg tccggccata gcaccggtat gacttgaacc agcacgagct
    19861 ccagcctgag aaccaccaac atattgagct ataatcggct tttttgatgt tattgttata
    19921 gccacattca gaaaacgttt tatatcacgt attccttcaa tatacaatgc aatagctttt
    19981 gtctgttcat cattagcaag gtattctaaa acatcaataa tggatatatc tgcttcattg
    20041 ccaacactta cagctttact caatcgtatt ccatgattct ttaaatagcg aagtgtttgg
    20101 gtaatatatg taccgctctg ggtagcaaat cccagtgaac ctggcctgtc aatatattcc
    20161 atcaccgtgg tattcaatga tatttcagta ttgacaatac ccatgcagtt tggacctaaa
    20221 aatctgatgc cgtttatgcc tgctgttgat ttaagcttat cctctaatag ttttccttcc
    20281 tgaccaatct ccttaaatcc tgcgctgatg ataatagctc ttttggtacc tattgtacca
    20341 aagtctttca aagtatcggg aatattttgt gcaggtatca caaagaatgc gcaatcaaca
    20401 gcctcaggta aatcaagtgg cgacttgtac gttttcagtc caaaaactga ttcctcttta
    20461 gggtgaataa catagattct ccccttatac ccatctttca taatgctgag taaatgcagt
    20521 gttgccatct ttaatggatt gctgctggct cctaccactg ctattgaaga aggattaatc
    20581 aatttttcaa gtggattcat gctatcctcc atttgttttt tatgcattac gacatggcat
    20641 acttattttg caatataaaa atgaataatc gttatttaat ttatgg
//
LOCUS       DAOOJH010000037        19284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2822_length_19284_cov_9.607104, whole genome shotgun sequence.
ACCESSION   DAOOJH010000037 DAOOJH010000000
VERSION     DAOOJH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19284
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2822_length_19284_cov_9.607104"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1104)
                     /locus_tag="PLJ75_06645"
     CDS             complement(109..1104)
                     /locus_tag="PLJ75_06645"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPD04932.1"
                     /translation="MNKYIAQVAVVTLLVFFTYSAFAQENGLTSPPSSDIPVSDIPDN
                     NTAVTNTPDATVNNSTSSWNPFTKGNGPTTPTLAMFIAVPVITTVYGMETWDWGEQKN
                     PELADEGFFGKNTDSGGADKAGHAYAHYLSFRVFHAYYDWSENGRNTKWFYSIATATF
                     LGVFIEVGDAFTGKYGFSYEDVVADVAGIGLGILLEYSPTLDSLIGFSWQYWPTEGYT
                     DRWGANPLHMTSDYSGAKYVLNFRLAGLQNLGLRLPNFLRYIQLDFGYFTRNYVRWDK
                     TDDDPYRSLYFGISLNFMEVVKDFFDNPESRTCKILQQPFKYYHIPAGYSGDYKI"
     gene            1418..1810
                     /gene="queF"
                     /locus_tag="PLJ75_06650"
     CDS             1418..1810
                     /gene="queF"
                     /locus_tag="PLJ75_06650"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPD04933.1"
                     /translation="MTDYSKANDAVIEKVQGYIFDTIPARGHETIVRWEYPEFQSLCP
                     VSGRHDQGTVIISYKPAKLLLESKSVRNYLGAWRNKSIWQEYVTDVIAQDLFDAAKPQ
                     WIEVSIQWAPRGGIFATTTSVKGNKKKL"
     gene            1807..2802
                     /locus_tag="PLJ75_06655"
     CDS             1807..2802
                     /locus_tag="PLJ75_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD04934.1"
                     /translation="MMSEYIAGVDIGGTKIIATIADVTGIKSRVYQQTQLHGDNFTIP
                     KQVYALIQLAAENAQIQYDEIKSVGVSTCSPFLRNGVYKEIVAPNLCGGLTDSGRLPN
                     DWQSIALEEFLCNIYPHVIVENDCVASVVAEKLFGAGKFIDNLVYVTWSTGIGAGIYA
                     DGKLIRGKNGNAPHFGHIFIAEDGPQCGCGNYGDLEALASGSAIARDYHGSSAKIVFD
                     DYRKGNEKAIAIIKRAARNFARGLASLNAMVDTEYISLGGSVMNDSDILLPMIREEFY
                     QSFKVLSHNVIIEKSALFQYIADIAALALVMPASWIEQWRTYSPWLQSPGMIKLN"
     gene            complement(2870..3196)
                     /locus_tag="PLJ75_06660"
     CDS             complement(2870..3196)
                     /locus_tag="PLJ75_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD04935.1"
                     /translation="MDIKHEIKGNVLIVSIEGELTSPQALDVDKKIINEIEKEKKERI
                     IIDLSKLDFIDSTGLGVLVSIRLYCKKNKILFATVGVKEKVKRTFTVTEMTSFLNIHD
                     SMDKLL"
     gene            complement(3269..3898)
                     /locus_tag="PLJ75_06665"
     CDS             complement(3269..3898)
                     /locus_tag="PLJ75_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPD04936.1"
                     /translation="MYRIKGKHVVVIGAGEVAYRKVKDLIAAGAIVTVIAPHIGKDLS
                     ALANDHSVTIIQRLYQKGDCKGAALVFSATNNTEVNQQVFYEASELNIPINAVDDPDN
                     SSFYVPSFFTRGKLLVAVSTGGASPAMAAKVRRLLEQAIPQDIEQALDALTAIRKLLK
                     ENFPHLTSNERSTVLKTIANDEHLIARTIASHNNNTLHELLNELLMNKK"
     gene            complement(3921..5189)
                     /locus_tag="PLJ75_06670"
     CDS             complement(3921..5189)
                     /locus_tag="PLJ75_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD04937.1"
                     /translation="MIDIGKLYCGGSSTGDGLRYGTEASVDAHGNLPHTRVAMADQRK
                     PIVVWNVTRTCNLNCVHCYTDSHNKKYGELSTDEGLTLIDDLATFGIPSLLFSGGEPL
                     MRRDIFRLITKAADKNIRPVLSTNGTLIDRDKARAIKDAGIVYVGISLDGMEDVNDTF
                     RGMDGAFKKAMKGFENCIAVGQRVGLRLTLTKRNYADLHNIFDFIEKEGINRACFYHL
                     VYSGRGGSLFADDLSYSESRQAMDTIIERTDDFFRRGLDINILTVDNHVDGVYLYLKL
                     LQKDEQQAASIAQLLTWNGGGAYSTGVGIANIDFEGNVHPDQFWQDYSFGNILTRSFA
                     DIWMDESNPLMKGLKHKADYIKGRCRLCKFKPMCNGSMRVRAYRVFGDPWAPDPQCYL
                     TDEEIGLTHDTIIALKDAGEYYTMPEELAQ"
     gene            complement(5286..6263)
                     /locus_tag="PLJ75_06675"
     CDS             complement(5286..6263)
                     /locus_tag="PLJ75_06675"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04938.1"
                     /translation="MLDEKKLISQIQIAVPVTKRPYRVLADTLGTTEYEVINTVQKLK
                     QDGLIRSIAGIFDAKKLGYTSNLVAFAIPEPAIAHGVAIINALPGVSHNYLRDGAYNI
                     WFTLALPEETNFIETVQHLAEQVNAVTFNIFQAVLTVKLSARFFEDDSIHDNNAQYAV
                     TGKHNETIPVDDTVKNTVKVLQCDLPCSEEPFDQLIAQSNIPMDVDLFIATANRLLEN
                     GMMRSYRAVVKHHAMGFVANAMTVWKADGDKRNSMLEQFANENAISHLYVREAYPNPW
                     DYPVFAMVHAKSEDELDMIIKKLYSIAKTDYMSYRSLQEFKKERVRYFE"
     gene            complement(6253..7311)
                     /locus_tag="PLJ75_06680"
     CDS             complement(6253..7311)
                     /locus_tag="PLJ75_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD04939.1"
                     /translation="MNKPRLIFWELTKRCNLRCVHCRAESYDKEFEHELSLQDCKNIL
                     DSIAAYYKPIMVLTGGEPLYRKDLFDIAGYANGKGLPIALATNATLIDREIASAIKKS
                     GFERVSISIDGPDALTHDSFRGIPGSFDKALQGAQLLHEAGVPFQFNTTITRRNVTSI
                     EKILQLAKTMHAVALHIFMLVPVGCGVEIAQTDMLSKEQYEEVLRWFYHTTQEAGIEF
                     KATCAPHYYRIIRQEAKKENRKLTFETDGLAAVTRGCLAGSGVCFISHRGDVQPCGYL
                     PLVAGNCLLTPFNELWESSELFLKLRDLSNLEGKCGVCEYKAFCAGCRARAYYASGNY
                     MDEEPFCIYQPKRATHAG"
     gene            complement(7308..8594)
                     /gene="hemA"
                     /locus_tag="PLJ75_06685"
     CDS             complement(7308..8594)
                     /gene="hemA"
                     /locus_tag="PLJ75_06685"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPD04940.1"
                     /translation="MAECTQQIGMVGMSHKTASVETRECFAFDTAVLESFVDNAKKNN
                     IDEMVYIATCNRMELYFVSSQIEEAMQSVVQLLEQFAGLKREQFEPVLYKKFHKDVIR
                     HLLCVASSLDSMVIGENEIVGQVKNWYRWAAVHKTTGPILNKLFHQAFRTAKRIRTQT
                     EIARNPLSIAFIAVELARKIFDDLASSSVLLVGAGEMSELILKYLTKFKIGTVILANR
                     SLHNAQRIATEINRDATIIPLSEIIDVIPEVDIIITSTGSHEYILTRDMITEHIKKRS
                     QPLFLIDIAVPRNIEPAVSTIHDVFLYNIDNLKSIADENLKNRIHETKLAMELIDADA
                     EDFLHWYQSLEIVPLIVSIQETFSTIRNEELQKYRRRKLKHLSDDDFAIVEELTNQIM
                     SKTLHIPISYLKDLSDNYHKKESKEKTKLLQEMFKR"
     gene            complement(8600..9424)
                     /gene="ccsA"
                     /locus_tag="PLJ75_06690"
     CDS             complement(8600..9424)
                     /gene="ccsA"
                     /locus_tag="PLJ75_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPD04941.1"
                     /translation="MTLLVYLTFFTFIITSVYSILSIHRDASSYIRVTKVAIIVAIIL
                     LFFQGCFLYKTIAHAYKINIFTFFYLIVVFLSLLLLFMSISRWKDDFRALSAIITPLI
                     TVIYGISLLFYGSNRFMAIEPNHNLMLIHIVLSLLGEVLFFIAFASAVLYLVVSWQLQ
                     KKSSMKFIYRFPSLGVLNRLTNFSLAHSLAFFSAGIAIGIIMLFEVYHNISLGSPKEI
                     LMYAVWCILLIVWIIAHSKITSNFYVSIITIVAFVIVIVLFIATNVVITTGFHSFR"
     gene            9936..10922
                     /locus_tag="PLJ75_06695"
     CDS             9936..10922
                     /locus_tag="PLJ75_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04942.1"
                     /translation="MPMKDIIIFTSDEEKLVEHIVQAIESKYQTEYILIEKHKEALRS
                     LATSISLYPSLLDSQRLQNQKRTVQSLLDTLCSRSMPDMILHIPTKAILGRAFTIAKI
                     NFFIMLWYIARDKDEYTAFVEALLLCIASNVFMLTAEEVYTSIIEDDALDKIIRHNAV
                     YLLARTWEHRLDYGVAEFAPILLNLWKARERLIPNFGTMMGFSELCMLSEHTSSLWLD
                     FLQRDAIMEDEVYALEEFIFDLTYEEIAFVRDYLEKNSKTTVMREELPSIMGKTHIPE
                     YSGQDPRELYRSFRDRKINSRFRARSKLNGPKKTLEEYLMCFLLSSKTVVEY"
     gene            10955..11608
                     /locus_tag="PLJ75_06700"
     CDS             10955..11608
                     /locus_tag="PLJ75_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04943.1"
                     /translation="MFDIYGSYEYNPSIFDNMQMEINDIDDDISSDIENLLANRFDSL
                     YLKDIYKKNNICIDIIYPRVQYGHHRVLLKSPDYIRFLLSLYPFSSDLENLQKIVLRP
                     RHIILGNVELMALYLQQKKILVVYLHHPHFYSLKTSRFAQFSELTPMIHMLQGKIMKT
                     NMHENDPYLIPPLWYMVSIIESSSENKVDKFLLRCDSSSKLRAVLDEISFFYGRHGY"
     gene            11614..12396
                     /locus_tag="PLJ75_06705"
     CDS             11614..12396
                     /locus_tag="PLJ75_06705"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPD04944.1"
                     /translation="MIDYHVHTYLCDHASGTPGDYIEKALEARLFEIGFSDHAPLPEH
                     LRNDITMEPEETEMYCSMLEDVKDKYPIAVKIGFEVDYPLHPTFDTHYFHDNRIDYMI
                     GSCHFIDGWAFDHPLFVDEYNKRDINQVYRDYYSIVFDMVNSGLFDIIGHFDLVKKFG
                     YRATVDLTDHILPIVKLAGKQATAIEINTSGLRKPVKEIYPSYDIMKLMFENNVPITL
                     GSDAHAPEEVAYNFYDALLLIKRAGYKKIAGYSKRKQYSIIL"
     gene            12393..13115
                     /locus_tag="PLJ75_06710"
     CDS             12393..13115
                     /locus_tag="PLJ75_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HPD04945.1"
                     /translation="MITLKKDTNNIYRVYGLCNLQAGVIAKAVNTIDYSDTDNNVRFK
                     EKLLIYKATGILPEHIVMLDQVHGSSIIEMNYRPQKNDPFVTQADGFVTMVHGLCLVI
                     RTADCVPVILYDSAKGILAALHSGWRGCMQNITAEGIKKLYQKGSSPASVHVMILPSI
                     SRDAYEVGDDVASQFNGYILNKNGRCYLDMWNHIADTAMKNKIPCNNIYVTGICTFQN
                     NTEFFSYRKGDKGRNLNFVYLS"
     gene            13321..14235
                     /locus_tag="PLJ75_06715"
     CDS             13321..14235
                     /locus_tag="PLJ75_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04946.1"
                     /translation="MEKLQLSKLLDTHNHNKTLDEIKSLYRKYYSEFAIIEDLFFIVK
                     DLFYGEFPGYQKCNTQYHDFQHTVDATLAAMRLLDGYNIKCKIIPEDIAYCLIIAALL
                     HDTGYIQEDWDTEGTGAKYTAVHVGRSVEFLRKHYTKFQIAEKYIPIISLCIQSTGLS
                     TNFKELAFISKEHMIAGAMMGTADLLGQMSDREYLEKLLFLYYEFKEAGIPGYSTEYD
                     IIWKTIDFYVMTVKRMKRDYLNVQRYCTYHFEKRYAIPSNLYMVAINRNIRYIKKIIA
                     DESTNFRHKLKRGDQYQLQMLSQQSVHH"
     gene            14470..14997
                     /locus_tag="PLJ75_06720"
     CDS             14470..14997
                     /locus_tag="PLJ75_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04947.1"
                     /translation="MKKAISTIVMLKLVLLAANVWAYSVVTIQKWEINQNDVIKQYLP
                     NANYKQYTLNQIAGYNNKLLNYILAIDADYKNKITIFRTGKGIARDFMFIGTKLYVVT
                     DYMQLSNAESSSEVISALGKIYGTPQKQTEANTTTYTYTKDQTKIIAILEAKGQKYSM
                     TIYYYYKNLFKSLLQ"
     gene            15323..15598
                     /gene="rpsT"
                     /locus_tag="PLJ75_06725"
     CDS             15323..15598
                     /gene="rpsT"
                     /locus_tag="PLJ75_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003332298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPD04948.1"
                     /translation="MANIKSAEKRNRQNEKRRQKNSKIKSSVRTAEKKVRKSLENNSN
                     ETAQVLFKEFVKKIDAAAGKDILHWKTAARKKSRLAKKVNSVNAVSQ"
     gene            15614..15880
                     /locus_tag="PLJ75_06730"
     CDS             15614..15880
                     /locus_tag="PLJ75_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD04949.1"
                     /translation="MTKHEIITRLHIKHEIPRDVVQQIVDGFVEEIVQSVQNGEKVTI
                     KGFGVFTRIRKAERKVFSPIAQREVVVPSKYVIDFKSSKNTAIE"
     gene            15881..15953
                     /locus_tag="PLJ75_06735"
     tRNA            15881..15953
                     /locus_tag="PLJ75_06735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15914..15916,aa:Val,seq:tac)
     gene            15997..17340
                     /locus_tag="PLJ75_06740"
     CDS             15997..17340
                     /locus_tag="PLJ75_06740"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPD04950.1"
                     /translation="MKKLQEYLSPIEKVLLSFVIVIFSGAFLLCLPVCTTHGITFIDA
                     LFTSTSAVCVTGLVVLDTAKDFTFIGQVVILLLIQLGGLGIMTFSVALLSLIGSSISI
                     KWRFTLQNMYSDVSKLPVRHVLTRIVIYTFSIEACIAFILFTQFIQQFPFRVAIWHSI
                     FHSVSAFCNAGFSTFSSNLMDYNGNTVVIISIGLDIILGGLGFLTLSELFSNKTFKLK
                     KLWKALTIHSRLVLIVTAILIFSGMLLFLVIEYDGILKTMPLKEKLLASFFQSVTART
                     AGFNTVDIGALQEGTCLMLIVLMFIGGSPGSIAGGVKTSTVAVLYIYLISRLKGLKQI
                     VIGERALSYDNIERATTLVIVSGIFIFCTTFILVLIPTLPIPEFLASLFEVTSAFGTV
                     GLSMDFTPKTLPIERIILCIVMYAGRLGPLTLISALTSHRKTINIRYAEDHVMIG"
     gene            17347..18033
                     /locus_tag="PLJ75_06745"
     CDS             17347..18033
                     /locus_tag="PLJ75_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HPD04951.1"
                     /translation="MKKYFVIGLGNFGFNIARSLEENGCEVLGMDVNKDLVQRAKDSI
                     SHAIIGDASDREVLESLAISDFDGSIISIGQDMAASILIALYLKEIGIKKIIVRAISE
                     DHGKILSLIGVDTVVFPERDMAIRLANKLALKNAMDYLPITDEYGILEVIPPKTFINK
                     SLKDLKLSTRYGCQVIGLKYFPNGNYNDVSEKNVQIKIAPTADDVILENSVMIVIGKV
                     EDIERLQNAD"
     gene            complement(18034..18990)
                     /locus_tag="PLJ75_06750"
     CDS             complement(18034..18990)
                     /locus_tag="PLJ75_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD04952.1"
                     /translation="MKVKFWGVRGSIPTPLNSDVIRDKIKQALQLASPKDISNEESID
                     TFLDTLPISIVGTYGGNTTTLEVRTSTDDLIIVDCGTGIKQLGHELLKTEFGNGKGFA
                     TILLTHTHWDHIQGFPFFAPFYVKGNRFNIYSPYNDIKERIEYQQVYTHFPINLEYML
                     AQKEFFTIDKESEIYINDVKIKNQSMRHPGGSFGFRFEENGKSFVFTSDCEFNIDEID
                     KIDTYKNFFENADVCVFDTQYTFEESINKIDWGHSSASIAIDIASMFNIKRLILFHHE
                     PDYNDQKLDTVLSNAKLYMGINPDRRGNLTINIAREGMEIEI"
BASE COUNT         6021 a         3452 c         3703 g         6108 t
ORIGIN      
        1 ttttaccggg taaaaggcat ccattgcctc aaattctact ggggaccgcc accggggatg
       61 ctgcgaaaaa acctataacg ctctctataa caaataattg atcacttttt atattttata
      121 atcgccgcta tatcctgccg gtatatggta atatttaaac ggttgctgca aaattttaca
      181 ggttctgctt tcgggattat caaaaaagtc ttttaccact tccatgaagt ttagtgagat
      241 gccaaagtac aggcttcggt aggggtcatc gtcggtttta tcccagcgaa catagttgcg
      301 ggtgaaatag ccaaaatcaa gctgtatata gcgcaaaaag ttgggcaatc gtaaacccag
      361 attttgcagt ccagctaatc taaaattaag aacatatttt gcaccgctgt aatcgcttgt
      421 catgtgcaaa gggtttgctc cccagcggtc ggtgtagcct tcagtaggcc aatactgcca
      481 gctaaatcca ataagcgagt ccagtgttgg cgaatattct aatagtatac ccaaaccaat
      541 cccggcaaca tcggcaacaa catcttcata cgaaaatcca tatttgccgg tgaatgcatc
      601 gccaacttct ataaaaacac ctaaaaaggt tgctgtcgca attgaataaa accacttggt
      661 atttcttcca ttttcggacc aatcatagta ggcatgaaaa accctgaatg ataaatagtg
      721 tgcataggca tgtcctgctt tgtcagcacc accagagtcg gtattcttac caaaaaaacc
      781 ttcatcagca agctcgggat ttttttgttc gccccaatcc catgtttcca tgccatatac
      841 tgttgtaata accgggactg caataaacat ggctaatgta ggtgttgttg ggccattgcc
      901 tttggtaaaa ggattccacg aactggttga attgttaaca gttgcatcag gtgtattggt
      961 aacagcagta ttgttatctg gtatatcgct aacaggaata tcactggaag gcggcgaagt
     1021 taagccattt tcctgagcaa atgctgagta tgtaaaaaaa acaagaagcg taacaactgc
     1081 tacttgtgca atatacttat tcatggtcag tgtcctttat acactcatat agtatttaat
     1141 acgcaaaaaa aaatattgag tggtagactg tagattgcat ttttaaatgt caagataata
     1201 ttaacgatat tttttaaaaa aataactata caaataaacg tatcaatatt tataaaaaat
     1261 attcatgtca ccatcataac tgtacattaa aagtattatt ggcgatagtt actctataag
     1321 tatatgtcag gctgtaaata gtgtgttaca aaaaatactt gctaaaatag tacttcatgt
     1381 atcgtaataa aaacatatta atgtaagagt gttaaccatg actgattatt caaaagcaaa
     1441 cgatgctgtt atcgagaagg tgcaggggta tatctttgat actatccctg ccagagggca
     1501 tgaaactatt gtcaggtggg aatatccgga gttccagagt ctttgccctg tttcgggcag
     1561 gcatgatcag ggcaccgtga ttatcagcta taaacctgct aaattgttgc tggaaagcaa
     1621 atcagtgcgt aactatcttg gtgcatggcg taacaaatct atatggcagg aatatgttac
     1681 tgatgttata gctcaggatt tatttgatgc ggcaaagcca caatggattg aggtgtccat
     1741 acagtgggct ccgcgcgggg gcatctttgc caccacaacg tctgtaaaag ggaacaagaa
     1801 aaagctatga tgagcgaata tattgctggt gttgatatag gcggaacaaa gattattgca
     1861 actattgctg acgtaaccgg aataaaatca cgggtgtatc agcaaacaca gttacatggt
     1921 gacaatttta ccataccaaa acaggtgtat gcattaatac agcttgcggc tgagaatgca
     1981 cagatacaat atgatgagat taaatctgtt ggtgttagta catgcagccc ctttttgaga
     2041 aacggagtat ataaggaaat tgttgcccct aatttatgtg gtggtttaac agacagtgga
     2101 cgcttaccta atgactggca gtcaattgca cttgaagaat ttttatgtaa catatatccg
     2161 catgttattg tggaaaatga ctgtgtagca tcagtggttg ccgaaaagct ttttggtgca
     2221 ggtaaattta ttgataatct tgtttatgta acatggagca cgggtatagg tgccggtata
     2281 tatgctgatg gaaagcttat acgcggtaaa aatgggaatg cgccgcattt tgggcatatc
     2341 tttattgctg aagatggtcc gcaatgcgga tgtggaaatt atggggattt agaggcgtta
     2401 gcttcgggca gtgctattgc ccgggattat catggcagtt cagctaagat tgtctttgac
     2461 gattaccgta aaggcaatga aaaagcaata gcaataatta aacgagctgc tcgtaatttt
     2521 gcccgcggat tggcatcgct taatgcaatg gttgatacag aatatatcag ccttggcggc
     2581 agtgtaatga acgatagtga tattttactg cccatgattc gtgaagagtt ttatcaatca
     2641 tttaaggtac tatcccacaa tgtcattata gaaaaaagcg cactctttca gtatattgct
     2701 gacatcgctg cattagccct tgtcatgcca gcatcatgga ttgagcaatg gcgaacctat
     2761 agtccatggc tgcagtcacc aggaatgata aagctcaatt gatggttact cgatactttt
     2821 tgtttttaca atggcagtaa aaaataaaag cgtttaatgc ttgtgtatgc tacagcagtt
     2881 tgtccatcga atcatgaata ttgaggaacg atgtcatttc ggttacggta aatgtacgct
     2941 ttaccttttc tttaacgcca actgttgcaa ataaaatttt attcttttta caatataatc
     3001 taattgatac aagaacaccc aatccggtag aatcaataaa atccagtttt gacaaatcaa
     3061 tgataatccg ttcttttttt tctttttcga tttcattaat tattttttta tcaacatcaa
     3121 gcgcttgagg tgatgtcaat tctccttcta tagaaacaat aagaacgtta ccctttattt
     3181 catgtttgat atccatgaac aaataacctc ctgtacacaa tgtttatata caattacata
     3241 gaacagtaac gtatagtaaa atcaaggatt attttttatt catcaataat tcattgagca
     3301 attcatgcaa tgtgttgttg ttatgcgatg caattgtccg tgcaatcaga tgttcatcgt
     3361 tagctattgt tttcaacaca gtacttcgtt cattgctggt aagatgggga aagttttctt
     3421 tcaatagttt gcgtatggca gttaatgcat caagggcttg ttcaatatcc tgcgggatag
     3481 cttgttctaa gagcctgcgt accttcgcag ccattgcagg agatgcgcca ccggtggaca
     3541 cggctacaag cagtttacca cgagtaaaaa acgatggcac gtaaaatgat gagttatcgg
     3601 gatcatcgac ggcattgatg ggtatattta attcagatgc ttcataaaaa acctgctggt
     3661 ttacttctgt gttgttggtg gcagaaaaaa caagtgcagc acctttacag tcacctttct
     3721 ggtataagcg ctgtatgatg gtaacactgt gatcattggc aagtgcagat aaatctttgc
     3781 ctatatgggg agctatcaca gtaactatcg cccccgcagc aataaggtct ttcaccttgc
     3841 ggtaggcaac ctcaccggca cctataacca caacgtgttt gccttttatc ctgtacatta
     3901 ttggatacac catatgcttg ctattgtgct aattcctccg gcattgtata gtactctcca
     3961 gcgtccttta atgcaataat ggtatcatgc gttaatccta tctcttcatc agtaagatag
     4021 cactgcgggt cgggagccca tgggtcgcca aatacccgat aggcgcgcac acgcatggaa
     4081 ccgttgcaca ttggtttaaa cttgcacagt cggcatctgc ctttaatata atctgcctta
     4141 tgcttgaggc ccttcatcaa tggattgctt tcatccatcc atatatcggc aaaacttcgc
     4201 gttaaaatat tgccaaatga gtaatcctgc cagaactgat cgggatgaac atttccctca
     4261 aaatcaatat tggcaatgcc aacaccggtt gaatacgctc cgccgccatt ccatgttaaa
     4321 agttgcgcta tgctggcagc ttgttgctca tccttttgca gtagctttaa atatagataa
     4381 acgccgtcaa catggttgtc caccgtaagg atgtttatgt ccagtccccg tctgaaaaaa
     4441 tcatcagtgc gctcaatgat ggtatccatt gcctgccgtg attcagagta tgaaaggtcg
     4501 tctgcaaaca gtgaaccgcc acgccccgaa tagacaagat ggtaaaagca ggcgcggtta
     4561 atcccttctt tttcaatgaa gtcaaatatg ttgtgcaggt cggcatagtt gcgtttggtt
     4621 aatgtcaatc gtaatcccac gcgctgccca actgcaatac agttttcaaa gcctttcatg
     4681 gcttttttaa atgcgccatc catccccctg aatgtatcgt ttacatcctc cattccgtcc
     4741 aggcttatac caacatagac aatacctgca tcttttatgg cacgtgcttt atctctgtca
     4801 attaatgtac cgttggtgga tagtaccggg cggatgtttt tatctgctgc ttttgttata
     4861 agcctgaata tatccctgcg catgagtggc tcaccacctg aaaacaaaag cgagggaatg
     4921 ccaaatgttg ccaaatcatc aataagcgtt aacccctcat cagtagaaag ctcaccatac
     4981 tttttattat gcgaatccgt ataacagtgc acacagttta gattgcatgt tctggtaaca
     5041 ttccacacta caattggttt gcgctgatct gccattgcca cgcgcgtgtg aggaaggttg
     5101 ccatgcgcgt caaccgatgc ctcagtgcca taacgcagtc cgtcacctgt tgatgaaccg
     5161 ccacaataca atttgccgat atctatcatg gttacctcac attattacaa tcctctatag
     5221 gcattgttta acattacata aacgtattat caagcaccat tttaccatac tatttgtagt
     5281 cgctgttatt caaaatatcg tacccgttct tttttaaatt cttgtaatga tcgatagctc
     5341 atgtaatcag tttttgcaat tgaatagagt tttttgatga tcatatcaag ttcatcctca
     5401 ctttttgcat gaaccattgc aaagacagga taatcccatg ggttaggata agcttcacgc
     5461 acatacagat gcgatatggc attttcattt gcaaattgtt ccagcatgga gttacgttta
     5521 tcaccatcgg ctttccatac ggtcattgcg ttggcaacaa atcccatagc atggtgttta
     5581 accacagcac ggtatgatcg catcatcccg ttttctaaaa ggcggtttgc tgttgcaata
     5641 aacaagtcaa catccatggg gatattgctc tgggcaataa gttgatcaaa tggctcctcg
     5701 ctgcatggga ggtcgcactg gagcaccttc accgtatttt tcacggtatc atctactggt
     5761 atggtttcat tgtgtttacc ggttacagca tactgtgcat tattgtcatg gatactatcg
     5821 tcctcaaaaa aacgtgcaga cagcttaacc gtcaacactg cctggaagat attaaatgtt
     5881 acagcgttaa cctgttccgc cagatgttgc actgtttcaa taaaattagt ttcttctggc
     5941 agtgcaaggg taaaccatat attatatgca ccatcccgca ggtagttgtg gctgacaccg
     6001 ggcagcgcgt tgataatagc aacaccgtgg gcaatagcag gttcagggat tgcaaatgcc
     6061 accaggtttg atgtatatcc taacttttta gcatcaaata taccagcaat actgcggatc
     6121 agcccatcct gtttcaattt ttggactgtg ttgataacct catattcagt ggtccccagg
     6181 gtatctgcca atactctgta gggtcgcttt gttactggta ctgcaatctg tatttgcgaa
     6241 ataagttttt tttcatccag catgggttgc cctttttggt tggtatatgc agaaaggttc
     6301 ttcatccata tagttgccgg aggcatagta ggcgcgtgca cggcatcctg cacaaaatgc
     6361 tttatattcg catacgccgc attttccttc aagatttgat aaatctctga gctttaaaaa
     6421 aagttctgat gattcccata gttcattaaa aggagtgagt aagcaattgc ctgctacaag
     6481 tggaagatag ccgcatggtt gaacatcacc ccggtgtgaa ataaaacata cgccactgcc
     6541 agcaaggcac ccacgggtta cagcagcaag cccgtcggtt tcaaaggtta gttttctatt
     6601 ttctttttta gcttcctggc gtataatcct gtaatagtga ggtgcacagg tagccttgaa
     6661 ctctatgccg gcttcttgtg ttgtatggta aaaccagcgc aatacttctt catactgctc
     6721 tttcgatagc atatcagttt gtgcaatctc aactccgcaa ccaaccggta caagcataaa
     6781 tatatgaagg gcaaccgcat gcatcgtttt tgccagttgt aatatttttt caatggaggt
     6841 aacattgcgc ctggtaattg tagtattaaa ctggaacggt acacctgctt cgtgtaacaa
     6901 ttgtgctccc tgtaatgctt tatcaaatga accggggatg cctctaaatg agtcatgggt
     6961 tagtgcatca gggccatcaa tggaaatact aacacgctca aatccgcttt tctttatagc
     7021 tgaagctatt tctctgtcaa tcaatgtagc attggtggct aatgcaatgg gtaatccctt
     7081 gccattcgca tagcctgcta tatcaaaaag gtctttacgg tacaggggct caccaccggt
     7141 caacaccata ataggtttat aatatgctgc aatgctatct aaaatatttt tacaatcctg
     7201 aagcgatagt tcatgttcaa attctttatc atagctttcg gctctgcagt gaacacaacg
     7261 caggttgcac cgcttggtca attcccagaa gatcaggcga ggtttattca tcgtttaaac
     7321 atctcctgca gcaattttgt tttttccttt gattcttttt tgtgatagtt atctgataaa
     7381 tcttttaaat aagaaatagg tatgtgcaat gtcttggaca taatttgatt ggtcagctct
     7441 tcaacaatag caaaatcatc atctgaaaga tgttttaatt ttcgcctgcg atatttctgc
     7501 agctcttcat tcctgatggt tgaaaaggtt tcctgaattg acacaatcaa cggaactatc
     7561 tccagactct ggtaccagtg caaaaaatcc tctgcatcag catcaataag ttccatggca
     7621 agttttgttt catgtattct attttttaaa ttttcatcag ctatggattt tagattatca
     7681 atattgtaca aaaagacatc atgaatggtg ctcaccgccg gctcaatatt gcgtggaacg
     7741 gcgatatcaa ttaaaaatag tggctggcta cgttttttta tatgctctgt aatcatatct
     7801 ctggttaaaa tatattcatg tgatcctgtt gaggttatta ttatatcaac ttctggtatc
     7861 acatcaataa tttctgataa cgggataatg gtagcatcac gatttatttc ggtggctata
     7921 cgctgggcat tgtgcagtga cctgttggct aaaataacag ttccaatctt aaacttggtt
     7981 aaatatttta atataagctc actcatctca cctgcgccaa ctaaaagcac cgagcttgat
     8041 gcaaggtcat caaatatttt tcgtgccagc tcaacagcaa taaatgcaat tgatagggga
     8101 ttgcgggcaa tctcagtttg tgtgcgaata cgttttgctg ttcggaaagc ctggtgaaac
     8161 agcttattta agataggacc tgttgtttta tgaacagcag cccagcgata ccaattttta
     8221 acctgtccaa ctatctcgtt ttcgccaatg accattgagt ccaatgaaga agcaacgcac
     8281 aataagtgtc ggatgacgtc cttatgaaat tttttgtaaa gcactggttc aaactgctca
     8341 cgttttaaac cagcaaattg ctctaaaagt tgtacaactg attgcattgc ctcttcaatc
     8401 tgtgaagaaa caaaatatag ctccatacga ttgcatgtgg caatatatac catctcatca
     8461 atattatttt ttttagcatt gtcaacaaat gattcaagta cggctgtatc aaatgcaaaa
     8521 cactcccttg tctcaaccga tgcggtctta tggctcatgc caaccatgcc aatttgttgt
     8581 gtacattctg ccatgctgct tacctgaaac tgtgaaatcc tgtcgttatg acaacattgg
     8641 tagcaataaa taaaacaata acaatcacaa atgcaactat tgttattatg ctcacataaa
     8701 agttactggt aattttagaa tgtgctataa tccatacaat taataatata caccacacgg
     8761 catacatcaa aatctctttc gggctgccca atgaaatatt atggtatact tcaaaaagca
     8821 taataatccc tattgctatc cccgcgctga aaaaggccag cgaatgtgct aatgaaaaat
     8881 tggtaagcct gttgagtaca ccaaggcttg gaaaacgata aataaatttc atggatgatt
     8941 ttttctgcaa ttgccatgat actactaaat ataaaacagc agaagcaaat gctataaaga
     9001 ataaaacctc gccaagcaat gataaaacaa tatggatgag catcagatta tgattgggtt
     9061 caatagccat aaacctgttt gaaccataaa acagtaacga aatgccataa ataacggtaa
     9121 tgaggggcgt aataattgcg cttagtgctc taaaatcgtc tttccacctt gaaatgctca
     9181 taaaaagaag caatagagat aaaaaaacaa caataagata aaagaaggta aaaatattaa
     9241 ttttataagc gtgagcgata gttttatata aaaaacaacc ctggaaaaat aaaagaatta
     9301 tagcaacgat aatggcaact tttgttactc gtatgtatga cgaagcatcc ctgtgtattg
     9361 aaaggatact ataaacagaa gtaatgataa atgtaaaaaa tgtcaggtaa accagtagcg
     9421 tcatacagcc tcactatgtt actttcaata tatatcgttt ttataacaat tcataaatat
     9481 tgtcattgca cgatagtgtc aagcaataat gggcattcct gaacactatg caatagtaat
     9541 catctataca aactatctgt catattagga tgttattgca tcattacatg gtatgcaaag
     9601 tgctttgtcc tgttataaga tagtataatt ttatgtcatt tttttactaa attggcaaaa
     9661 atatatttga ctcttttatc tattttattt actattatta gtgaggctac tcattgagta
     9721 gttaccttcc aacataatcc gctaatgata ttgacgattc ctcacaagag gggtcgtcat
     9781 tttttttaca tttcttcttt atttactata cctatgttat tcatacaatg ctttatacta
     9841 actaaccatg tgccgcaatg gattttattc aaattaaata ttgacaaaat tatccacagg
     9901 aagtctaatc ttattaatgt taatgggtga tatctatgcc aatgaaagac atcataatat
     9961 ttacatctga tgaggaaaag cttgttgaac atattgtgca ggcaatagaa agtaaatatc
    10021 aaactgaata tattctcatt gaaaaacata aagaggcatt gcgcagttta gcaacatcaa
    10081 tttcgttata tccttcgctc cttgattcgc aacgtcttca aaatcaaaaa cgcaccgtgc
    10141 aaagtttgct tgataccctg tgttcacgtt ccatgcctga tatgatttta catataccaa
    10201 ccaaagctat actggggagg gcgttcacca tagccaagat caattttttt atcatgctat
    10261 ggtacattgc gcgtgataag gatgaatata cagcatttgt agaagcgcta ttattatgta
    10321 tagcttccaa tgtatttatg ctcacagccg aggaggtgta cacttctata attgaagatg
    10381 atgcacttga taaaatcata cgacacaatg cagtgtatct tttagcgcgt acatgggagc
    10441 acaggcttga ttatggagtt gccgaatttg cgcccatttt gttaaattta tggaaagcac
    10501 gtgaacgcct tatcccaaat tttggtacta tgatgggctt ttctgaatta tgtatgcttt
    10561 cagaacacac ctcgtcatta tggcttgatt ttttacagcg tgatgctatt atggaagatg
    10621 aggtatatgc acttgaagaa tttatatttg accttactta tgaagagata gcttttgtgc
    10681 gagattatct tgaaaagaat agtaaaacta ctgtaatgcg agaagaattg ccatcaatca
    10741 tggggaaaac acatatacct gaatatagtg ggcaggaccc ccgcgagctt taccgttcat
    10801 tcagagatcg caagattaat tccaggttca gagcacgatc aaaattaaat ggtcctaaaa
    10861 aaacgcttga agaatatctc atgtgctttt tattatcttc taaaacagtg gttgaatatt
    10921 aagcaatagc aaggagtatt atacagggaa aggtatgttt gatatttatg gttcatatga
    10981 atataaccca tccatatttg ataatatgca gatggagata aatgatattg atgatgatat
    11041 atcaagtgat attgaaaatc tgctggcaaa ccggtttgat tcattatacc ttaaagatat
    11101 ctataaaaaa aataatatct gtatagatat tatttacccc cgcgttcagt atggtcatca
    11161 cagggtgttg ttaaaatcac cggattatat acggtttctt ttgtcattat atccattttc
    11221 cagtgatctt gaaaacctgc aaaagattgt tttgcgccca cggcatatta tattgggcaa
    11281 tgttgagctc atggctttgt atctgcaaca aaaaaaaata ctggtagtat acctgcatca
    11341 tcctcatttc tattcactaa agacatcacg gtttgcccag ttttccgaat taacacccat
    11401 gatacatatg ctgcagggta aaataatgaa aacaaacatg catgaaaatg atccctatct
    11461 tataccacca ttgtggtata tggtatcaat tattgaatca tcatctgaaa ataaagttga
    11521 taaatttttg ttgcggtgcg atagttcttc gaaattgcgg gcagtactgg atgagatatc
    11581 cttcttctac gggcgtcatg gatattaaac accatgatag attaccatgt acatacctat
    11641 ctgtgcgatc acgcatcagg tacgcccggt gattatattg aaaaagcgtt ggaagccagg
    11701 ctctttgaaa ttggtttttc tgatcatgca ccgttgcctg aacatttgcg gaatgatatt
    11761 accatggaac ctgaagaaac cgagatgtac tgctcaatgc ttgaagatgt aaaggataaa
    11821 tatcccatag cagtgaaaat aggttttgag gttgactatc cgcttcaccc aacatttgat
    11881 acccattatt ttcatgataa tcgcattgac tatatgattg gttcatgtca ttttattgat
    11941 ggctgggctt ttgatcatcc cctgtttgta gatgaataca ataagcggga tatcaatcag
    12001 gtttatcgtg attactatag tattgttttt gacatggtca attcagggct ttttgatata
    12061 attggccatt ttgatcttgt aaaaaagttt ggttacaggg caactgttga tctaaccgat
    12121 catattcttc ccattgtaaa gcttgcagga aaacaggcaa cagccattga aataaatacg
    12181 tcagggttac gcaaaccggt gaaggaaatt tatccgtcgt atgacattat gaagcttatg
    12241 tttgaaaaca atgttcccat tacactgggt tcagatgctc atgcacctga ggaggttgcc
    12301 tataattttt atgatgccct gcttttgata aagcgggcgg ggtacaaaaa aattgcagga
    12361 tacagtaaac gaaagcaata ttcgataata ctatgataac attaaagaag gacacaaata
    12421 atatctacag ggtttatgga ctgtgcaatt tgcaggcagg tgttatcgca aaagctgtca
    12481 atactataga ttattctgat actgacaata acgtccgctt taaagagaaa ttacttatct
    12541 ataaagctac agggatattg cccgaacata ttgttatgct tgaccaggtt catggcagca
    12601 gtatcattga gatgaactat cgcccacaaa agaatgaccc ctttgttaca caagctgatg
    12661 gttttgttac catggtacat ggattgtgcc ttgttatacg aacggctgat tgcgttccgg
    12721 ttatactgta tgattcggca aaaggtattc tggcagcgtt gcattccggc tggcgcggat
    12781 gtatgcaaaa tattactgct gaagggataa aaaaattata tcaaaaaggt tcttcacctg
    12841 cttcagtcca tgttatgata ctgccgtcaa taagccgtga tgcgtatgag gttggggatg
    12901 atgttgcttc ccaatttaac gggtatatac ttaacaaaaa tggcaggtgc tatttagata
    12961 tgtggaatca tattgctgac accgcaatga aaaacaaaat tccgtgcaac aatatatatg
    13021 taacggggat atgcacgttc caaaacaata cagaattttt ttcatacagg aaaggagata
    13081 aaggtagaaa tttaaatttt gtatatttat catagcaata gttaattttt agcctgtgaa
    13141 aataaatata aacctgcatt gtaataaata caatgccaat ttattcagag attgtgatat
    13201 gggatattgc tttttacaag agcatttata tatggttaat ttttttaaaa aaactgaata
    13261 aaagcttgca ttttttaaaa aaaataaaat attatattaa taagtagtca ggaggaaagt
    13321 atggaaaagc ttcaactgtc aaaacttctt gatacgcata atcacaataa aacattagat
    13381 gaaataaagt cattatatag aaaatattat agtgaatttg caataattga agatcttttt
    13441 tttattgtta aagatttatt ttatggcgaa tttcctggtt accagaaatg taatactcaa
    13501 tatcatgatt ttcagcatac cgttgatgca acattagcag caatgcggct gttagatgga
    13561 tataatatta aatgtaagat aataccagaa gatattgcat attgccttat tattgctgct
    13621 cttttacatg ataccgggta catccaggaa gattgggata ccgaaggaac aggagcaaaa
    13681 tatactgcgg tacatgtagg gcgcagtgtt gaatttttac ggaaacacta tacaaaattt
    13741 caaatagctg aaaagtatat ccctataatt tcattgtgta tacagtcaac agggctgtcc
    13801 acaaatttta aggagcttgc atttatatca aaggaacaca tgatcgccgg ggcgatgatg
    13861 ggaactgccg accttttagg gcagatgtca gacagagaat atttggaaaa gctgctattt
    13921 ttatactatg aatttaaaga ggcaggtatt cctggttatt ccaccgagta tgatatcata
    13981 tggaaaacta tagattttta tgtcatgaca gtaaagcgca tgaagagaga ttatctcaat
    14041 gtgcagcgtt attgtacata tcattttgaa aagcgatatg ctattcccag taatctttat
    14101 atggtagcaa taaaccgcaa tattagatat atcaaaaaga taattgctga tgaaagcaca
    14161 aactttagac ataaattgaa acgtggtgac cagtatcagc ttcagatgct ttcacaacaa
    14221 tcggtacatc attaatattt taatgtgctt atgcaatgca aatggcagga tacgtgtgtt
    14281 gtattatcaa tgtcctcgga taaaaagata aaagaacccg tagaaattgc gatgatatat
    14341 aagccatagt aagggcttat ttgatatacc acctgctacc agagcataat ggaaagaaaa
    14401 atgtttgaca taatatgttc atctgggtta ttttttaaca ctatagtgat aaagcaaaaa
    14461 ggaatagata tgaaaaaagc aataagcaca attgttatgc tgaaacttgt cttgctggcg
    14521 gcaaatgttt gggcttatag tgtggttacc atacagaagt gggaaattaa tcaaaatgat
    14581 gttataaaac aatatctgcc aaacgcgaat tataaacagt atactcttaa tcagattgct
    14641 ggatataata ataaactttt aaattatata cttgccattg atgcagatta taaaaataag
    14701 atcacgattt ttagaacagg caagggtatt gccagagatt ttatgttcat aggaacaaaa
    14761 ttgtatgttg taactgacta tatgcaacta tccaatgctg aatcttcttc tgaagtaatc
    14821 tcagcgctgg gaaaaatata tggaacacca cagaaacaaa cagaagcaaa cacaaccacc
    14881 tatacctata caaaagatca aacaaaaatt attgcgattt tagaagctaa agggcaaaaa
    14941 tattcaatga ctatatatta ttactataaa aatttattta aaagcctgct gcagtaagta
    15001 gtaattgtgg gttttcatgt aacaatgtct atggcgatat tttgtaagta ctcagaaagt
    15061 agaggcacat tcaggggaaa atatagattg tttgattaag cttctactta atagttcatt
    15121 taaaggtaga ataatataag tcttttttaa acggtccaaa atatattctc aaaaagaaat
    15181 gttaagtttt ctacaggttt tttatgaaaa tttttattaa attcttgaca caatgtggta
    15241 caatctgcat atatcctttt cattgaatct tcaatataat tgataatctt aaattatact
    15301 ttttacatat gagggtgtta cattggctaa tataaaatca gctgagaaac ggaatagaca
    15361 aaacgaaaaa agaaggcaaa aaaattctaa gattaaatca tctgtcagaa ctgctgaaaa
    15421 gaaagtgcgc aaaagccttg aaaacaattc aaatgaaact gcacaggtgt tgtttaaaga
    15481 atttgttaaa aaaattgatg cagcagcagg taaggatata cttcactgga aaaccgcagc
    15541 acgcaaaaag tcacgattag caaagaaggt taatagcgtt aatgctgtat cacaatgacg
    15601 cagagggtaa tttttgacca agcacgaaat aattaccaga ttgcatatta agcatgaaat
    15661 cccccgtgat gtagtacagc aaattgttga tggttttgtg gaagaaatag tgcaatcggt
    15721 tcaaaatggc gagaaggtta ctattaaggg gtttggcgtt tttacaagga tcagaaaggc
    15781 ggagcgcaag gtgttttcac caattgctca gcgggaagtg gtagtaccgt caaagtatgt
    15841 tattgacttt aagtccagta aaaacactgc tattgaatag gggcgcttag ctcagctggg
    15901 agagcatctg ccttacaagc agagggtcag aggttcaaat cctctagcgc ccacagtaat
    15961 tggggtatac aggcgtatac cccaatttat agtgctatga aaaagttaca agaatatcta
    16021 tcgccaattg agaaggtcct tttaagcttt gttatcgtaa tattttccgg tgcttttctt
    16081 ttatgccttc ctgtttgcac aactcacggc atcacattta tcgatgcatt atttacttca
    16141 acatcagcag tttgcgttac tggcttagtg gttttagata ctgctaagga tttcaccttt
    16201 attggacagg tagtcatctt gttgcttatc cagttaggtg gtttgggcat aatgacattt
    16261 tcagttgctt tactttcctt aataggtagt agcatatcca ttaaatggcg ttttaccttg
    16321 caaaacatgt atagcgatgt atcaaaactt cctgttcgcc atgttttaac aaggattgtc
    16381 atctatacat ttagcatcga ggcatgtata gcttttatac tgtttactca atttattcag
    16441 cagttcccat ttcgtgttgc tatctggcat tctatatttc attctgtttc tgctttttgc
    16501 aatgctggtt tttccacgtt ttccagtaat cttatggatt acaatggtaa taccgttgtc
    16561 attatcagta taggtctgga tattatttta ggtgggcttg gatttttaac gttgagcgag
    16621 ttattttcca acaaaacatt caagctaaag aaactctgga aagctctgac tattcattca
    16681 agactggtat tgatagtgac agctattttg atatttagtg gtatgttgct gtttcttgta
    16741 atagaatacg atggcatact gaaaaccatg ccgttaaaag aaaaattgtt agcatcgttt
    16801 tttcaatcgg taacggcccg aacagcgggt ttcaatacag tagatattgg tgctttgcag
    16861 gaaggcacat gcttgatgtt aatagtattg atgtttattg gtggctcgcc cggttctata
    16921 gctggcggtg tgaaaacaag tactgtggct gtattataca tatatcttat atcccgttta
    16981 aaagggctta aacagatagt tataggggaa agagctttgt catatgataa tattgaaagg
    17041 gctacaacgc tggtcattgt atcgggaatt tttatttttt gtactacgtt tattcttgtc
    17101 ttaataccta cattgccaat acctgaattt cttgcatctt tatttgaggt tacttcggca
    17161 tttggtacag ttgggctgtc gatggatttc actcccaaaa ctttacctat tgaacggatt
    17221 attttgtgta ttgttatgta tgcaggacgg ttgggaccat tgacattgat ttcggcattg
    17281 acatcacata gaaaaacgat taatatccga tatgcagaag atcatgtaat gatagggtag
    17341 ttaaatatga aaaaatattt tgtaatagga cttgggaatt ttgggtttaa tattgctcgt
    17401 tcattagaag aaaatggatg cgaagtattg gggatggatg ttaataaaga tctggtccag
    17461 cgagccaaag attctataag tcatgcaatt attggcgatg cttcagacag ggaagtttta
    17521 gaatcattgg caataagtga ttttgatggt tctattataa gtataggaca ggatatggcg
    17581 gcaagcatat tgattgcctt atatcttaaa gagattggca tcaaaaaaat tattgtaaga
    17641 gctatctcgg aagaccatgg aaaaatatta tcacttattg gtgttgatac ggtggttttt
    17701 cctgagcgag atatggcaat aaggcttgct aacaaattag cactaaaaaa tgcaatggat
    17761 tatcttccta ttactgatga atatggaatt ctggaagtta tacctccaaa aacctttata
    17821 aacaaatcat tgaaggattt gaaattaagt acccgatatg gatgccaggt aataggctta
    17881 aaatattttc caaatggaaa ttataatgat gtaagcgaaa aaaatgttca aattaaaatt
    17941 gcgccaacag cagatgatgt catactggaa aattcggtta tgatagttat tggtaaagta
    18001 gaggatattg aacgtttaca aaatgctgat tgattatatt tcaatttcca ttccttcgcg
    18061 tgcgatattt attgttaaat tgcctcttcg atcaggatta attcccatgt agagttttgc
    18121 atttgataga acagtatcaa gcttttgatc attataatca ggttcatggt ggaatagaat
    18181 taatcgcttg atgttaaaca tgctggcaat atctattgca atagaagcag atgaatgtcc
    18241 ccaatctatt ttatttattg attcttcaaa tgtatactgc gtgtcaaata cacatacatc
    18301 cgcattttca aagaaatttt tatatgtatc tatcttatca atttcatcaa tattgaactc
    18361 gcaatcactt gtaaaaacaa atgatttgcc attttcttca aacctgaacc caaatgaacc
    18421 acctggatgc cgcatgcttt gatttttgat tttgacatcg ttaatatata tttcactttc
    18481 cttatcaatt gtaaaaaatt ctttctgagc aagcatatat tctaaattaa ttgggaaatg
    18541 ggtatagacc tgctgatatt caattcgttc tttaatatca ttatatggtg aataaatatt
    18601 gaatcggttg cctttaacat aaaaaggggc aaaaaatgga aaaccctgta tatgatccca
    18661 gtgggtatga gtaagtaata ttgttgcaaa acctttacca ttaccaaatt cagtctttaa
    18721 taattcatgt cctaattgtt ttattcctgt accgcaatca acaataatta aatcatcagt
    18781 agaggtgcga acttccagtg ttgttgtatt acctccatag gtgccaacta ttgatatggg
    18841 caatgtatca aggaatgtat cgatagattc ttcattggag atatcttttg gtgaagctaa
    18901 ttgtaatgcc tgttttattt tgtctcttat tacatctgaa tttaatggag ttgggattga
    18961 acctcgaacg ccccagaatt ttactttcat taaatttttc tcctcaatat aatatggtta
    19021 tataaaatat atccatataa agaaatcaac taataatttt ttataaggct ctggccccaa
    19081 tttctttctt attttttcat gtgtaatcca attttcaatg tgatatgtat gtacttacgc
    19141 agacacagaa catcgcagat acagaacata tgctctgacc ccattatttc ctgtacaatg
    19201 caagatatgc attgttagat gtttgttgct gattatttgt atgatattat gggttatata
    19261 aataatttat aatgtttcgt ctca
//
LOCUS       DAOOJH010000038        18884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2888_length_18884_cov_10.718891, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000038 DAOOJH010000000
VERSION     DAOOJH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18884
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2888_length_18884_cov_10.718891"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLJ75_06755"
     CDS             complement(<1..216)
                     /locus_tag="PLJ75_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD04953.1"
                     /translation="MSTQHKLIIGNCMSMEEIPDESIHLMVTSPPYFNAPFDYKELFK
                     NYDHYLGVLTQVAQEIYRVLQDGRIAVL"
     gene            complement(335..763)
                     /locus_tag="PLJ75_06760"
     CDS             complement(335..763)
                     /locus_tag="PLJ75_06760"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPD04954.1"
                     /translation="MAYQVSVFAENKPGKIERITNVLASQNINIRAITINDSGDYGII
                     KLLLDRPEDGCAALKDEGIAATLKEIVAVKIQDRPGSLHKVAAILKEHSINVDDAYGF
                     TIKPHEESAFVFQVDNPKAAEKVLRDSGYQLLADKELYLL"
     gene            complement(904..2199)
                     /locus_tag="PLJ75_06765"
     CDS             complement(904..2199)
                     /locus_tag="PLJ75_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPD04955.1"
                     /translation="MYWNNNIETISRKDLESLQLTKLNKALQAASVTPFYKKVFEENN
                     INIDAIKTLEHIKDLPFTTKNDLRLSYPDGMLAVPKKKVVRMHASSGTTGKSTVIFYT
                     AKDIRDWADLVARCMVATGASQDDVFQNMMGYGLFTGGLGLHYGAEMLGCMVIPSSSG
                     NTLRQIQLMQDFETTIIHITPSYCLHMVEAIRAEGIDPYALTLKKAYLGAEPYSEATR
                     KKIEEQFGIDAYNSYGLSEMNGPGVAFECTYKNGMHIWEDYYIAEIINPETGEVLQPG
                     EEGELVLTHINREAMPILRYRTRDLTRIIPEPCQCGRTHYRIDRIKGRTDDMLIVSGV
                     NVFPSQIETVLMKFPEIGNNYQIILDRENNLDRMHIKVELYSKLFHGDIKEIDRIKSK
                     LKEALKALITINPRIDLLEPGSLPPSEGKAKRVFDNRTL"
     gene            complement(2345..3196)
                     /locus_tag="PLJ75_06770"
     CDS             complement(2345..3196)
                     /locus_tag="PLJ75_06770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPD04956.1"
                     /translation="MQTQYRFGGPITTSLKKIIVFTSVVYIIQLFGNIVAPGVIEYTF
                     GIHHIGFIHEFKLWQPVTYMFLHGNFFHIFFNMFALWMFGCDLEELFGSKNFMRFYLY
                     SGIGAGIGIAFMNYYIYATNGYNPVTIGASGAIYAILLAYGMIWPNREVLLYFIFPIK
                     MKYLVLLFGGIEFFGTIASAQGLGGNVSHIGHLGGFITGFILLKTRFWLRQKTSFNPF
                     ASISRNYRLAKHKKRIDDRIKAKEIIDTLLDKITHYGYNSLTPEEKQKLEWARRHYYP
                     DNNDIIH"
     gene            complement(3299..4900)
                     /locus_tag="PLJ75_06775"
     CDS             complement(3299..4900)
                     /locus_tag="PLJ75_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04957.1"
                     /translation="MDNAKPQAIATTVLVVDDEESIRDILSRYLQLKGYTVLTAGNGH
                     TAMDILLSQAVDLVLTDLNMPNVNGRELLKRMAEKFPDIPKIVLSGYGSNEDMLVALK
                     SGAYDFIQKPLTDFGILDKSVERAIERKKLKEERDRYVQQITRLNEIIGLLNKGAAFE
                     EIFDIIAKSLNNVIPFDRLAVAIINDAGQVETRLVSSTKNILININDKFPLSDSSLLK
                     VSETKQYHIINDLEEYYRQNPSSERTKLLLQEGMRSSMTIPLIIANRLKGFLLFASVQ
                     PNTFNNDHIQFAETISGHIAFSIQRGELLHELDMHNKKLEQIVKIRTHQLLKTQKATI
                     FALSSLTELRDPETGYHLDRMRGYCMLLAQIAKYSGVALEISNQYLRDLYDSSILHDI
                     GKVGIPDNILLKPGKLTYEEFEIIKTHTTIGYNALKRASQEMGQDSFLSMAMDVTLYH
                     HEAWDGTGYPYGLSEDEIPLSARIVTIVDVYDALTTIRPYKEAYSHQKSIEIMKSEAH
                     RYDPHLFELFLQNADDFDAIRQKYQ"
     gene            5245..7482
                     /locus_tag="PLJ75_06780"
     CDS             5245..7482
                     /locus_tag="PLJ75_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04958.1"
                     /translation="MISIQKFESWAIKPFNAIFNFFSERIAYTFGIAFTTSLGGFIIA
                     AYIALFLIATVRFNVSNTGSDFVMLFSGLTLFVYAPLYYLHFGLLYKAGIPCFTRRLS
                     LINKYVEHNTLHQTMSDEEALQLLDALEHLPRDNMAAALIYPSIVMVVCCLYEAIKGT
                     IANALFLLLGIASAIFIYIFLTYIVAELLSGRMRRKIKWLLVKRGISFDERYILSIRG
                     KFIFINIMVMVAMFELGAMVYYNNYELFSIIPLMFISITGILVFVILFFYLLSIEESL
                     GEIESAAVDLARGGSGLLFLPYLDKELINTSKGFIAAAYEVNDIRKNLEQKVEERTEE
                     LNKALTSLKEKNDLIAKELRIAANIQQGILPQTPSVHNAVKIVAYNRASSDIGGDLYD
                     IIQMQDNCLGVLIADVSGHGIPAALISTMVKISFRESCTNYLLPRRIFADVNKQLVNI
                     IRTQEYATVFFMVIFPTYDVMYANASHRKAVVYKRQSGTIMYWDTGGIFLGALPDEEV
                     GSLFEDNFGILEPGDRVFLYTDGLPESRNWNNEEFSDERVERLVSETVNMSIEEARDY
                     IITEWEKFLGGTPPRDDMTFVFLELDPILYNALQHRERGEQLVHEKKFNLAIEELKKA
                     VIMNPQDINALLLLSRSYIYIHHYDRALPHLIDYIAKKPDNSFAHYLLAIVHYKKNQF
                     EEAYNAASQALTMQSSYIPALYSMAAICKALGRKDDAIQYCKQILKLDAGNIKAQRLL
                     QKLLP"
     gene            complement(7479..9293)
                     /gene="pepF"
                     /locus_tag="PLJ75_06785"
     CDS             complement(7479..9293)
                     /gene="pepF"
                     /locus_tag="PLJ75_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020589653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HPD04959.1"
                     /translation="MDNTVKKIPRRDEIPDNDKWDVTPLFASAEEWEKLYAEIENSIH
                     DYATFEGKLHQSFSIFLQAIEFDLSISRSIDRLFTYAHLKSDEDTTNQDAQAMLQKAH
                     SLYQRVSEASSFLVPEIQSIPDTIMSNYLSNHKLTPYVFFIEKILRYKEHTLSKEIEE
                     ILAMAHEIAIAPAEFFKKLDNADLRFGTIEDEQGNTIELSHGNFITFLMSYNRDVRKK
                     AFHAYYEVYANHKFSIAQSLASSVKKDVFYSRVRRFKSCLHQALFPDNIPETVYHNLI
                     DSVGKNLNPLFHYLHYRKEALGLDELHFYDTYVPLIPDVPFTMEYQEAVETCIEALTA
                     LGDDYTSVLHDGLLSGWVDRYENRGKISGAYSSGCYDSPPYILLNYDNTINSLYTLIH
                     ESGHSMHSYFSRKYQPYVYHEYTIFVAEVASTLNEMLLSHYLLNKYQDNPLMQAYILN
                     REIDEIRGTLFRQTMFAEFERIIHTMAENNQPLTYQALTEAYAALLQKYFETTIVIDP
                     ELPMEYLRIPHFYSAFYVYKYATGIAAALSLAQMILSSDDGAKKYMQFLRLGGSMFPL
                     EELKIAGVDMESPDAIANALHYFDNLVNTFIQVHKSLV"
     gene            complement(9469..10866)
                     /gene="nhaC"
                     /locus_tag="PLJ75_06790"
     CDS             complement(9469..10866)
                     /gene="nhaC"
                     /locus_tag="PLJ75_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPD04960.1"
                     /translation="MRKRLPSFSECIALLTITIALLFAGTVLFKIPIPLLLIVAASIS
                     GVKSYLLGHSWAEIEESIVNGIKGTLPAIMILMTVGILSGSFIASGTIPLLIDYGLKL
                     IHPGWILPAAYIVCSISSLVIGSSWTTAATVGVALVGMANAMHVSPAICAGAVISGAY
                     FGDKLSPLSDTTNLAPGVSDTDLFSHIRAMMFTTIPAWVLAFIGFLIINLYSVPNNNT
                     QSEQIVSLLQTLETSFNLNPVVITPLMLVIIMALCKFPAVVILLSGSTVAILFGWLVQ
                     SQNLLFVLQSSYSGFVIHTSNEIINNLLNRGGILSFLETILIILCATGLGGIWESGGY
                     ITSIHNNILHKIKTSKMLIISTVLTGVISNIIMAEQYLSIVMTGRIYKDAYKRKNLEP
                     YMLSRSLEDGGTVTSALVPWNSCGAFMSSALGVSTLHYLPYTLFNLIMPVVAIVFTAL
                     GFFIYKKNNIKQHNK"
     gene            11135..11926
                     /locus_tag="PLJ75_06795"
     CDS             11135..11926
                     /locus_tag="PLJ75_06795"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPD04961.1"
                     /translation="MHIFDQDIALVETGNNAFKATISENWLINTIPNGGYLIALLSNA
                     MLRQSDKKTTPIITANYIQRCKPGEANVHIECFGQSSQFNRYQASLFQQGNEKIRAWG
                     TFASNNNNQYIERYECKSPDVAAIRDCLQLPETPAFQVLSHVAIRLDPYSWGWTHGEV
                     HDRSIQKGWLAFREQRTYDIPALLLMADVIPPAVFVSLGLVTWVPTIELSINIRNMPH
                     SQWLKFYSRTRFITCGLLETDGEIWDEEERLVLLSRQIAQIRVQR"
     gene            12040..13605
                     /locus_tag="PLJ75_06800"
     CDS             12040..13605
                     /locus_tag="PLJ75_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HPD04962.1"
                     /translation="MKKYCIGSMIICLMSINFLYAETIKPEITKVIVYHDSAMIKKEA
                     KMMLNKAVNEIVITNLPAAMVDESVQVIVKDAALQDVRLETTYLQKGDAKRINTVLEK
                     LNAIKDDITAKQNEIASINSVLEYIKKGSTSPFSIKMTAAEMNSILQLIEQYSNQSYI
                     KIAKLQQALQKLNQEKERLEKELALLNANEASKTLKLTVYNTGNNNATVLLQYITGNA
                     GWDMEYDMRTDTEKSATDITCNALVAQNTGEDWNNVAVELSTAKPFVATQLPQLNPWY
                     LDIYRPVYYKESAKSMAVQDADALSRAPSPEEMPEMQQETIAISFLVKGHKTIPSDGN
                     RYKLAVASSVTGCTLRYTTIPKIFAYIMLEGEFVNPFDFPLQRGSVNVYLDGKYVNSF
                     SLDKIYVPKDTVTIGLGVDESMAVERKLVSKKTEYKGIVNKTKRIEYVYSITLNNGKK
                     RDITVTVHDAIPVSQNEKIEVILINKDTIGATIDSQGKATWNIILGPNQKKELKVHFT
                     VEYPDKVQVAGLE"
     gene            13612..14751
                     /gene="rlmD"
                     /locus_tag="PLJ75_06805"
     CDS             13612..14751
                     /gene="rlmD"
                     /locus_tag="PLJ75_06805"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPD04963.1"
                     /translation="MKKHRKLLYTVAQQYADKHVSPSCQHAGECGGCLLQHITYDAQL
                     LIKKDYCNKVLEGIASIEEVMPSHPLGYRNRMDYVTAFGRMGLRKRGNWRHVVDLEDC
                     LLLQTGSRALFTTIRQKLAGIEDYDYHTHRGYLRYIVLREGFNTGQVMVNFVVARHEN
                     NCKAIIDAIADRVTSMSLVLNDGLTDLSYGRVIEDIKQGYIEEKLNGICYRITPNSFF
                     QANTHVTQMMYTEIAKHVYGRALDIYCGVGSISLFIANNVETVTGVECNPEAVMIAKK
                     NALINAVPNAVFIESDAMNYISTYSDDYDVIVVDPPRTGLEQKVVQKLLDIHAKRIVY
                     MSCNPITFASDAGAIMQEYTLQFCKAYDMFPQTQHVELLAVFDRT"
     gene            14754..15917
                     /locus_tag="PLJ75_06810"
     CDS             14754..15917
                     /locus_tag="PLJ75_06810"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPD04964.1"
                     /translation="MQLSNSQKAIQRRVYHHVIKRMHTFFTIVQPGFEQIAFNELLHM
                     GIKPLAITKGGIVFRGNLYDSYKANMFSRVATRIIMRLTEFRALHFNEIYRTLRNFPW
                     ELYISSGTSIAIEVSVHKSKLHHTDRIGKEVLNAIEKRMNDFALHINTDQYRVQTIYI
                     RFDHDQCGVSMDTTGDPLYKRGYKQHVGRAPLRETIAAALLYECDITSYDVIIDPMCG
                     SGTFPLEAWCMTNNFFPGINRAFAFMHWPSFRKEACNSLASSLSRGNYGATILAGDRD
                     PSMVTIAKNNCTILGAAITTYSGDFLTEKLTIEGKHILCTMNPPYGKRIADDDTAGIY
                     SRLSYIFENWYPAWSFCIIIPLSLRHVFTVPVTKEIRFNNGGLPVIALIGRER"
     gene            complement(15889..16410)
                     /locus_tag="PLJ75_06815"
     CDS             complement(15889..16410)
                     /locus_tag="PLJ75_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPD04965.1"
                     /translation="METIRFESLPFVQDILLHPAFNTLKIFAHHGTISCYQHTIAVAQ
                     LAYQWSRLLHLDYIAATRGALLHDFYMYNWRTGGPRLHGLRHPHIALNNAAKHFVISK
                     KEKDIILKHMWPLTPIPPRYGESWVVTLADKMVSLHDYACVLKDKIIAFNIFFSQKLS
                     KLWRAIAPFQSEQ"
     gene            complement(16413..17156)
                     /locus_tag="PLJ75_06820"
     CDS             complement(16413..17156)
                     /locus_tag="PLJ75_06820"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HPD04966.1"
                     /translation="MHDYQLMLFEFIIFSTAGWILEVIYRSLNNKKIVNPGFLTGPYL
                     PIYGIGAVIITTIAYATISMPIIYKCIIYFLALSVLEYVSGVLLYILFGKRYWNYLDE
                     PLNIQGFVCLPFSIIWGLLALAFEYVLLPMFIHFYTTIPETLVNTIAFSGLTIMIIDC
                     LLTHSVVQKLINAKKTPSFLQHTMAIRFQNDALLTLRYFVDNAISYRKKINKMIPPKT
                     LRLVSSIKERYTMFLQSITKMIDNHNKHK"
     gene            complement(17149..17724)
                     /locus_tag="PLJ75_06825"
     CDS             complement(17149..17724)
                     /locus_tag="PLJ75_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD04967.1"
                     /translation="MIKRKQHQILRTAEMMFNRFGVKKTAVDDIAHDARVAKATIYNY
                     FGSKEGLLKALILEKIQQFDQKLQELLTANTQPIEKMKTLIREHIQLFINNPFIKDSS
                     LHDEDFSEELNASEQHMLQNIISEASKAGLLHNANSQQIINTMQLFLKGIEATLKNSI
                     TYSLDSLESSIDFISSSIFKSNPIHGDKTNA"
     gene            18173..18748
                     /gene="purH"
                     /locus_tag="PLJ75_06830"
                     /pseudo
     CDS             18173..18748
                     /gene="purH"
                     /locus_tag="PLJ75_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
BASE COUNT         5962 a         3281 c         3735 g         5906 t
ORIGIN      
        1 taaaactgct atacgtccat cctgtaaaac acggtatatt tcttgtgcaa cttgagttaa
       61 aacaccaaga tagtgatcat agtttttaaa aagctcctta taatcaaaag gcgcattaaa
      121 atagggtggt gaggtaacca tgaggtgtat gctttcatca ggaatttctt ccatggacat
      181 acaattgcca ataattagct tgtgttgtgt agacattgtt aaccttttag caaataatga
      241 aattacttat ttaaatattc attgtattga gaattaaaaa aatagcatgc caaacattgc
      301 atgaaaagct aaatctaccg attgggaagt atccttacag taaatataat tctttatcag
      361 caagcaattg atatcctgag tctcgtaata ctttttctgc tgctttagga ttgtctacct
      421 gaaacacaaa tgctgactct tcatgtggtt tgatagtaaa tccataagca tcgtcaacgt
      481 taatagaatg ttcttttagt attgcagcaa ctttatgaag actgcctggc ctgtcctgta
      541 ttttcacagc aactatctct ttcaatgtgg ctgcaatacc ttcatctttt aatgcagcac
      601 acccatcctc tggacgatcc agcaaaagtt taataatacc gtaatcaccg gaatcattga
      661 tggtaatagc cctgatatta atattctggc ttgcaagaac attggttatt cgctcaattt
      721 ttcctggttt attttctgca aacacagata cctgatatgc catagtgcac ctccataact
      781 gcataattat tatcgatagt gttaataaca gcttttttca tgcatacaac atttaacaaa
      841 aagattttac aatgttttgc actctcaagt atattaaaaa gtaacgtttt gaatattcca
      901 tatttataac gtacggttgt caaaaacacg tttagctttc ccttcactgg gtggcagtga
      961 gcctggctct aataaatcaa tacgaggatt aatagtaata agtgctttca gtgcttcttt
     1021 gagtttactc ttaatgcgat caatctcttt aatatcaccg tgaaaaagtt ttgagtatag
     1081 ctcaacctta atatgcattc ggtctaaatt attttcacgg tccagtataa tctgatagtt
     1141 attgccaatt tcagggaact tcataagaac agtttctatc tgtgaaggga atacattcac
     1201 gccacttaca atgagcatat cgtccgtacg ccctttaata cgatcaatgc gataatgggt
     1261 gcgcccacat tggcatggtt cgggtataat acgggtgaga tcgcgagtgc gatatcgtaa
     1321 aattggcata gcttcacggt taatatgggt taatacgagc tcgccttctt caccaggttg
     1381 taatacttca cctgtttcag gattgattat ctcggcaata tagtaatctt cccagatatg
     1441 catgccattt ttataggtac actcaaaagc aacaccaggt ccgttcattt cgcttaagcc
     1501 atatgaatta tatgcgtcta taccaaattg ttcttcaatc tttttacggg ttgcttcaga
     1561 atatggctca gcgcccagat atgctttttt caatgttagc gcgtatggat caatcccttc
     1621 agcacgtatt gcttctacca tgtgcagaca atagcttggt gtgatgtgta taatggttgt
     1681 ttcaaaatcc tgcattaact gaatctgacg cagcgtattt cctgaggatg acggtattac
     1741 catacaccct aacatctcag caccataatg taatcccaat ccaccggtaa aaagcccata
     1801 gcccatcata ttctggaaaa catcatcttg tgatgcacca gtggcaacca tgcagcgtgc
     1861 aaccagatca gcccagtcac gaatgtcttt agcagtgtaa aaaataacag tggatttacc
     1921 tgttgttccc gaggaagcat gcatgcgcac aacctttttt tttggtactg ccagcatgcc
     1981 atcgggataa ctcaggcgaa ggtcattctt tgttgtgaat ggcaagtctt tgatatgctc
     2041 cagtgttttg attgcatcta tattaatgtt attttcttca aatacctttt tataaaaggg
     2101 ggtaacgctg gcagcctgca gtgctttgtt tagttttgta agctgcaatg attcaagatc
     2161 ttttctgctg atagtttcaa tattgttatt ccagtacatg gcaacctctt ttaaaaatgt
     2221 acacgcattt actgtgctat agaagtggct ctacgatcca tttctacgat ggtaatgctg
     2281 tgacattgtc actgcttcca taggttgtca agaagatatt gatttacagt aactatcgcc
     2341 gccattaatg aattatatca ttgttgtcag gatagtaatg tctgcgtgcc cattctaatt
     2401 tttgtttttc ttcaggtgta agcgaattat agccataatg ggtaatttta tcaagaagtg
     2461 tatcgataat ctcctttgcc ttaatgcgat catcaattct ttttttatgt tttgcaaggc
     2521 gatagttcct ggaaattgac gcaaatggat taaatgaagt tttttggcgt aaccagaatc
     2581 ttgtttttaa aagaataaat cccgtaataa aaccacctaa atggccaata tggcttacgt
     2641 taccacccag cccctgtgct gatgcgatgg tgccaaaaaa ttctatgcca ccaaaaagta
     2701 aaactaaata tttcatcttg attggaaaaa taaaataaag aaggacctct cggttaggcc
     2761 agatcatccc atatgccaga aggattgcat agatggcgcc tgaagctcct attgttacag
     2821 gattgtatcc attggtagca taaatataat aattcataaa agcaatccca atccctgcgc
     2881 caataccact gtacaggtaa aagcgcataa agtttttact accaaaaagc tcttctaaat
     2941 cacagccaaa catccataga gcaaacatat taaaaaatat atgaaaaaaa tttccatgta
     3001 aaaacatata cgtaacaggt tgccacagct taaattcatg aataaatcca atatggtgga
     3061 tgccaaatgt gtattcgatt acaccgggcg caacaatgtt gccaaaaagc tgtatgatat
     3121 agacaacgct ggtaaaaact ataattttct taagtgatgt tgtaataggt ccaccaaagc
     3181 gatattgtgt ttgcatgatt ttccccgatt taatgatagt acaagtcttt attaagaaat
     3241 atagtttatg ctgcaataaa tgcaaaattt tgtttcaatg atactactgc cgtccaattt
     3301 actgatattt ctgtcggatt gcatcaaagt catctgcatt ttgtaaaaac agttcaaaaa
     3361 ggtgtggatc atatcgatgt gcttcacttt tcattatttc aatagatttt tgatggctat
     3421 atgcttcttt ataggggcgt atggttgtga gtgcatcata aacatcgaca atggttacta
     3481 tgcgtgccga taatgggatt tcatcttctg aaagcccata aggatatccc gttccatccc
     3541 atgcctcatg gtgatataac gtaacatcca ttgccatgga taaaaatgag tcctgtccca
     3601 tctcctgcga agcacgtttc aatgcattat agccaatggt tgtatgggtc ttaattattt
     3661 caaattcttc ataggttagt ttccctggtt ttaacaatat gttatccggt attcctacct
     3721 tgccaatatc gtgtaaaatg gatgaatcgt ataaatcgcg aaggtattgg ttgcttattt
     3781 ccaatgcaac tccagagtat ttggcaatct gagcaaggag catgcagtag ccgcgcatac
     3841 ggtcaagatg gtagcccgtt tcaggatcac gaagctcggt taaactgctg agtgcaaata
     3901 ttgtagcctt ctgagtttta agcaattgat gtgtgcgtat ctttacaatt tgttccaact
     3961 ttttgttatg catatcaagt tcatgaagca gttcgccacg ctgtatactg aatgcaatat
     4021 gtccgcttat ggtttcagca aattgaatat gatcattgtt aaaggtattg ggttggacag
     4081 atgcaaaaag cagaaaacct tttaatcggt tggcaataat gagtggtatg gtcatgctgg
     4141 agcgcatacc ttcctgtaaa agaagttttg ttctttcaga tgaaggattt tgcctgtagt
     4201 attcttctaa atcattgatg atgtgatatt gctttgtttc agaaactttt agtagcgaag
     4261 aatcgcttag aggaaactta tcattaatat ttattaaaat attttttgtt gatgatacaa
     4321 gacgtgtttc tacctgcccg gcatcattga tgatagccac tgccagacga tcaaatggaa
     4381 ttacattgtt aagacttttt gcaatgatat caaaaatttc ttcaaatgca gcacccttgt
     4441 ttaacaatcc tattatttca tttaaccggg taatttgttg cacatagcga tcacgttctt
     4501 ccttaagttt ttttctttca atagctctct caactgattt atccagtatt ccaaaatcgg
     4561 ttaacggttt ctggataaaa tcataggctc cgctttttaa ggcaacaagc atatcctcat
     4621 tgctaccata gccgctaagc actatcttgg gaatatcagg aaatttttcc gccatccttt
     4681 tcaggagttc ccggccgttc acattgggca tgttaaggtc tgttaataca agatcaaccg
     4741 cttgtgatag aagaatgtcc atggcagtat ggccattgcc agcagtaagt acagtatatc
     4801 cttttaattg aagatagcgt gaaaggatgt cccgtattga ttcctcgtca tcaacaacca
     4861 gtaccgtggt tgcaatagct tgtggttttg cattatccat gatttttaag ggtaactcac
     4921 attgtaatac atgtatttct tatacagtat ggtgcttttg gagttacgtc aatcgtaaaa
     4981 ttatagaaat acctaaaaaa ataggtacca tcaaatatag aaggattcat ctataaaatc
     5041 gattgttcac cttataaata atggggaaat gagttctgac gctctttttt ttacccggga
     5101 cgagcatgac gatgaacaat ttttagatgg tgagtattat tgtaattatc ataagatttt
     5161 ttttgattga tttttttgca taaatattac actttaaaac taaatactaa atatctaaaa
     5221 tactgtgcgt ttcaggacat cattatgatt tcgatacaaa aatttgaatc atgggcaata
     5281 aagccgttta atgctatatt caattttttt agtgagcgta ttgcgtatac gtttggcata
     5341 gcttttacaa caagtttagg cggctttatc attgcggctt atatagctct ttttcttatt
     5401 gcaacagtaa ggtttaatgt cagcaataca ggatcagatt ttgtgatgct cttttcaggt
     5461 cttacgttat ttgtttatgc tccgttatat tatttacatt ttggcctgtt gtataaagca
     5521 ggcatcccat gctttacaag gaggctttca ctcatcaata aatatgttga acataatacg
     5581 ttgcatcaaa caatgagtga tgaagaagcc ctgcaacttc ttgatgcact ggaacatctt
     5641 ccacgtgata atatggctgc tgcattgatt tatccttcca tagttatggt tgtttgctgt
     5701 ctgtacgaag caatcaaagg aactatcgcc aatgcgcttt tccttttatt gggtattgca
     5761 tcagcaattt ttatctatat atttttaacg tatatagtag ctgaactttt gtctggaaga
     5821 atgcgtcgca aaataaaatg gttactggtg aaacgtggca tatcatttga tgagcggtat
     5881 atcttgagta tcaggggtaa atttattttc atcaatatta tggttatggt tgccatgttt
     5941 gaattgggtg ccatggtgta ttataacaac tatgagttgt tttcaataat accgctgatg
     6001 tttatatcaa taactggaat acttgtgttt gttattttgt tcttttattt actttctatt
     6061 gaagaatcgc taggcgagat tgagtcggcg gcggttgatt tagcccgcgg gggaagtggt
     6121 ttgctctttt tgccctatct tgataaagag cttatcaata ccagtaaagg atttattgcc
     6181 gctgcctatg aggttaatga catacgaaaa aatcttgaac aaaaagttga agaaagaaca
     6241 gaagaattga ataaagcgct aacaagcctt aaagaaaaaa atgatttaat tgcaaaagag
     6301 ctcagaatag ctgcaaacat acaacagggt attttgccgc aaacaccttc tgtgcataat
     6361 gcagtcaaga ttgtagcgta taacagggca tccagcgata ttggtggtga cctttatgat
     6421 attatacaga tgcaggataa ttgtttaggt gtccttattg ctgatgtttc agggcatggt
     6481 atccctgctg ctttgataag taccatggtt aagattagct tccgtgaatc gtgcactaac
     6541 tatttgctac ccagaagaat ctttgctgat gtcaataagc aactggtcaa tataattcgc
     6601 acacaggagt acgctacagt attttttatg gtgatatttc ctacctatga tgtcatgtat
     6661 gcaaacgctt cacatcgtaa agcagtggta tataaacggc aatcaggaac tataatgtac
     6721 tgggataccg gtggtatctt tctgggggca ttgccagatg aagaggttgg gagccttttt
     6781 gaggataact ttggaatact ggagcctggt gacagggtgt ttctatacac tgatggtttg
     6841 cccgaatcgc gaaattggaa taatgaagag tttagcgatg agcgtgtgga aaggcttgtt
     6901 tcagaaacag tgaacatgtc aattgaggag gcaagggatt atattattac agagtgggaa
     6961 aaatttcttg gtggaacacc tcccagagat gacatgacat ttgtctttct ggaattagac
     7021 ccaattttat ataatgcatt acaacatcgc gaaagagggg aacagcttgt tcatgaaaag
     7081 aaatttaatt tagcaattga agaactgaaa aaagcagtca ttatgaatcc tcaagatata
     7141 aatgccctat tattgctctc acgttcgtat atttatatac atcactatga cagggcactt
     7201 cctcacctta ttgattatat tgcaaaaaaa cctgataaca gttttgcaca ctatttgctg
     7261 gctatagtcc attacaaaaa aaatcaattt gaagaagcat acaatgctgc atcacaggca
     7321 ttaaccatgc aatcatcata tattcctgca ctttatagta tggcagctat ctgcaaagca
     7381 ttagggagga aggatgatgc aatacaatat tgcaaacaaa tcctgaagct ggatgcaggg
     7441 aatataaaag cacagcgact attacaaaaa ctattacctt aaacaaggga tttatgtacc
     7501 tgaataaatg tattgaccag gttgtcaaaa taatgtaatg cattggcaat agcatcaggg
     7561 gattccatat ccacccctgc tatcttcaac tcttctaacg gaaacatact tccgcccaat
     7621 cgtaaaaatt gcatatactt ttttgccccg tcatcactgc taagtatcat ctgagccaat
     7681 gataatgctg cagcaatacc cgttgcgtac ttatatacat aaaatgcaga ataaaagtgt
     7741 ggaattctaa ggtactccat gggtaattcc ggatcaatga caatagttgt ttcaaaatat
     7801 ttttgtaata atgctgcata ggcttctgtt aaggcttgat aggttaaagg ctgattattt
     7861 tcagccattg tatggatgat acgttcaaat tcggcaaaca ttgtttgtct gaacaatgtc
     7921 ccgcgtatct cgtctatttc acggttaaga atatatgcct gcatcagtgg gttgtcctga
     7981 tacttattta atagatagtg gcttaagagc atttcgttta atgtggaagc tacctcagca
     8041 acaaaaatag tatattcatg gtatacatag ggctgatatt ttcgtgaaaa atatgaatgc
     8101 atcgagtgcc ctgattcgtg aattaaggtg taaagactat tgatggtgtt atcataattt
     8161 aaaagaatgt aaggtggtga atcataacag cctgatgaat atgctccgct tatctttccg
     8221 cgattttcat atcggtcaac ccaccctgat aacagtccat catgtaaaac agaggtgtag
     8281 tcatcaccca gtgctgtcaa tgcttctatg catgtttcaa ctgcttcctg atattccatg
     8341 gtaaagggaa catcaggaat taagggaaca taggtatcat aaaagtgcag ttcatccaac
     8401 ccaagagctt ctttgcgata gtgtaaataa tgaaaaagag gattgaggtt ttttcccaca
     8461 ctgtcaatta agttatgata caccgtctct ggaatattat cagggaacag agcctgatga
     8521 agacagcttt taaatctgcg tacccgtgag taaaatacat ctttttttac cgagcttgcc
     8581 agtgattgtg caatgctgaa tttatgattg gcatacacct cgtaataggc atggaaagct
     8641 tttttgcgta catcccgatt atagctcatt aaaaatgtga tgaaattgcc gtgacttaat
     8701 tcaatggtgt tgccctgttc atcttcaatt gtgccaaacc gcagatccgc attatccagt
     8761 tttttaaaaa attctgcagg ggcaatggct atttcgtgtg ccatggctaa tatttcttct
     8821 atttcttttg ataatgtatg ttccttgtag cgaagtatct tttctataaa gaatacatac
     8881 ggcgtaagct tgtggtttga cagatagtta ctcataatag tgtcaggtat ggattgaatc
     8941 tcgggcacta aaaagcttga tgcctcactg acacgctgat acaacgagtg agctttttgc
     9001 aacattgcct gagcatcctg attggttgta tcctcatcgc ttttgagatg tgcataggta
     9061 aacaatctgt caatggagcg cgagatggaa agatcaaact caatagcctg taaaaaaatt
     9121 gagaaggact gatggagctt gccttcaaaa gtagcataat catgtataga attttcaatc
     9181 tctgcataaa gcttttccca ctcttctgct gaagcaaaca gtggtgtcac atcccattta
     9241 tcattatctg gaatttcatc ccggcgtggt atttttttta ccgtgttgtc catggtagct
     9301 cctcattaca gtttttaaga tttttattaa taatggtata ttcatgtata atcgttatta
     9361 aaaatattgt acagtgtgcc ctgcagcaat gatatgaagt atttgcaagt gattctttgc
     9421 ctccatataa aacaattgtg tcatttaaaa cattatcgta ttattgtatc atttattatg
     9481 ctgttttata ttatttttct tatagataaa aaagcccaga gctgtaaata ctattgcaac
     9541 aacaggcata atgagattaa ataatgtata gggtaaataa tgcagtgtgg aaacacctaa
     9601 tgcggagctc atgaaagcgc cacatgaatt ccatggtaca agcgcagatg taacggtgcc
     9661 accatcctca agagagcggg aaagcatata tggctcaaga tttttccttt tgtaggcatc
     9721 tttatatatt cgccctgtca taacaattga taaatactgt tcagccataa taatatttga
     9781 aatgactcca gttaaaacag tggagataat aagcattttg cttgttttga tcttatgaag
     9841 aatattgttg tgaatggatg taatatatcc gccactttcc catatgccac ccaggcctgt
     9901 tgcgcacagt atgatgagga ttgtttctaa aaaactaagt ataccgccac gatttaatag
     9961 attattgatg atctcattgc tggtatgaat aacaaatccg gagtatgatg attgtaaaac
    10021 aaaaagtaaa ttttgtgatt gtacaagcca gccaaacagg atggcgacag tactccctga
    10081 taacaggatt acaacagctg gaaatttaca taatgccatt ataatgacaa gcataagtgg
    10141 agttatgaca acaggattca aattgaaaga agtttccaaa gtttgtagta atgacacaat
    10201 ttgctctgat tgggtattgt tgttgggaac actatacagg tttataatta aaaagccaat
    10261 aaaagcaaga acccacgcgg ggattgtggt aaacatcata gctctgatat gggaaaataa
    10321 atctgtatcg gatacacccg gtgctaaatt ggttgtatca gaaagaggtg atagtttatc
    10381 gccaaaataa gcacctgata taacagcacc agcacatata gctggtgaaa catgcattgc
    10441 atttgccatc ccaaccagag ctacgccaac agttgctgcg gttgtccatg aacttccaat
    10501 gacaagactg cttatactgc aaacaatata tgcggcgggt aaaatccatc ccggatgtat
    10561 gagttttagt ccgtaatcaa tgagtaaagg aatagttcct gatgcaataa aactacctga
    10621 taatattccc actgtcatga gtatcatgat tgcaggcagt gtgcctttta tgccattgac
    10681 gatactctct tcaatctccg cccagctatg gcccaataga taacttttaa cacctgaaat
    10741 tgatgctgca acgatgagta acagcggtat cggtatttta aatagtactg ttcctgcaaa
    10801 aagcaaagca atcgtaattg ttagcagtgc aatgcattca gaaaatgatg gtaatctttt
    10861 tctcatactt ttttatacta tatgtttgcc aatttatagt ttgcaataaa aggtggtgta
    10921 tgataacgaa tatcatagag tataatgatg atttaacgac ttgatggcaa tcataaaaaa
    10981 agcttttaat aataagtata ctggtattac aatgacaaaa tataaatatt gagctaccat
    11041 ggtgatgata cgttataaaa attgtcaaat agaaatagtt agaagctgta tgttatgata
    11101 ctatttttta gaatgttaaa agtgtggaca acatatgcat atttttgatc aggatatcgc
    11161 ccttgttgaa acaggcaaca atgcatttaa agcaactatc tcagagaatt ggttaattaa
    11221 tactatccct aatgggggtt atcttattgc attgttgagc aatgcaatgc tacgacaaag
    11281 tgataagaaa acaactccta tcataacagc aaattatata caacgttgca aacctggaga
    11341 agctaatgtg catattgaat gctttgggca gtcttcacaa tttaataggt accaggcaag
    11401 cttgtttcag cagggtaatg aaaaaataag agcgtgggga acgtttgcca gtaataacaa
    11461 taaccagtat attgaaagat atgaatgcaa gtcacctgat gtagctgcta tacgggattg
    11521 ccttcaactt ccagagacgc cagcatttca agtgttatcc catgttgcta tacggcttga
    11581 tccctattca tgggggtgga cacatggaga agtgcacgac aggtcaattc aaaaaggatg
    11641 gcttgcattc cgtgaacaaa gaacatatga tatccctgca cttttgctta tggcagatgt
    11701 aatacctcct gcagtatttg taagtttagg gcttgttaca tgggttccaa ccattgagtt
    11761 gtcaattaat atacgtaata tgcctcactc acagtggtta aagttttatt cgcgtacacg
    11821 gttcattacc tgtggcttac ttgaaaccga tggcgagata tgggatgaag aagagaggct
    11881 tgtgctgctc tcacggcaga ttgcacagat acgagtacaa cgatgagatt atttaagtaa
    11941 ctatcgtaca aagagatgtt taaaaaaatt ctgcccgggt ggcagacaat attcatcatg
    12001 atagttaaaa cgatattaaa ttatataagg agcgaatgta tgaaaaaata ttgtattggg
    12061 tcgatgatta tctgtttaat gagtattaat tttctttatg ccgaaacaat aaagcctgaa
    12121 ataacaaagg taattgtata ccatgatagt gctatgatta aaaaagaagc aaagatgatg
    12181 ctgaataaag cggtaaatga aattgtcatc acaaacttac ctgctgcaat ggttgatgaa
    12241 tctgtgcagg ttattgtgaa ggatgccgct ttgcaggatg tgcgtttaga gacaacctat
    12301 ttgcaaaagg gcgacgcaaa acgtattaat acagttttgg aaaagctaaa cgcaataaag
    12361 gatgatataa cagcaaaaca gaatgagata gcttcaatta attcagtgct ggaatatatc
    12421 aagaaaggca gtacttcgcc attttccata aagatgaccg ctgctgaaat gaattctata
    12481 ttacaattga ttgagcaata ttccaatcaa agttatatca aaatagcaaa gttacagcaa
    12541 gcactgcaaa agctcaatca ggaaaaagag cgtctggaaa aagaactggc actgctcaac
    12601 gcaaatgagg caagtaaaac gctgaaatta actgtataca atacagggaa caacaatgca
    12661 acagtacttt tacaatatat caccggtaat gctggctggg atatggaata tgacatgcgg
    12721 acagatacag aaaaatctgc tacagatatc acatgcaatg cccttgttgc tcaaaatacc
    12781 ggtgaagact ggaacaatgt tgcagtggag ctatcgacgg caaaaccctt tgtagcaact
    12841 caacttcctc agctcaatcc atggtatctg gatatttatc gccctgtata ttataaggag
    12901 tctgcaaaaa gcatggcagt acaggatgca gatgcattgt ccagagcacc ttctcccgaa
    12961 gaaatgcctg aaatgcaaca ggagacgata gctatttcat ttcttgttaa agggcataaa
    13021 acaatacctt ctgatgggaa tcgttataag cttgctgttg ccagctcggt tacaggttgt
    13081 acattacgat acactaccat tcctaaaatt tttgcatata tcatgttgga gggggaattt
    13141 gtaaatccat ttgattttcc tctccagagg gggagtgtga atgtttattt agatggtaaa
    13201 tatgtaaact cgtttagcct tgataagata tatgttccta aagataccgt aaccattgga
    13261 ttgggtgtcg atgaaagtat ggcggtagag cgtaaacttg taagcaaaaa aactgaatat
    13321 aagggaatag tgaacaaaac aaaacgtatt gagtatgtgt attcaattac actgaataat
    13381 ggaaaaaagc gtgatataac ggtaacagtt catgatgcca ttcctgtatc acaaaatgaa
    13441 aagattgaag tcattcttat aaacaaagat actattggag caactatcga tagtcaaggg
    13501 aaagcaacat ggaatattat ccttggtcct aaccagaaaa aagagttaaa agttcatttt
    13561 acagttgaat atcctgataa agtgcaggtg gcaggtttgg agtagaaaac attgaaaaaa
    13621 catcgtaagc ttttatacac tgttgcacag cagtatgcag ataaacatgt atcaccatcc
    13681 tgtcagcatg ctggtgagtg tggtgggtgt ctattgcaac atattaccta tgatgctcag
    13741 ttgttgataa aaaaagacta ttgcaataaa gtgcttgaag gtatagcatc cattgaagaa
    13801 gtgatgccat cacatccatt gggatatcgc aatcgtatgg attatgtaac tgcttttggc
    13861 aggatgggac ttagaaaacg gggtaactgg cgtcatgttg ttgatctgga agattgtttg
    13921 ctattacaga ctggtagcag agcgctcttt acaacaatac gacaaaagtt ggcgggtatt
    13981 gaggattacg attatcatac ccataggggg tacctgcgat atattgtttt acgtgaagga
    14041 tttaatacag ggcaggtgat ggttaatttt gttgttgcgc ggcatgaaaa taattgtaaa
    14101 gcgataattg atgcaatagc ggatagggtg acttccatgt cactggtatt gaatgatgga
    14161 ttgactgatc taagttatgg aagggtgatt gaggatataa aacagggata catagaagaa
    14221 aagcttaatg ggatttgcta tcgtattacc cccaattcgt tttttcaggc taacacccat
    14281 gttacacaga tgatgtatac ggagattgca aagcatgttt atggcagagc tcttgatata
    14341 tactgtggcg taggaagtat tagccttttt atagcaaata atgttgaaac tgttaccggg
    14401 gtagagtgta atcctgaagc agtaatgatt gccaaaaaaa atgcattgat aaatgcagta
    14461 cccaatgctg tttttattga atcagatgcc atgaattata tcagtacata ttctgacgac
    14521 tatgatgtaa tagttgttga cccaccacga acgggattgg aacaaaaggt agtgcaaaaa
    14581 cttctggata tccatgctaa aaggattgta tatatgtcgt gcaatcctat aacctttgct
    14641 tccgatgcgg gagcaataat gcaggagtat acattgcaat tttgcaaggc gtatgatatg
    14701 tttcctcaga ctcagcatgt agaacttctg gctgtatttg acagaacata atcatgcaac
    14761 tgtccaattc acaaaaagca atacaacgga gggtgtatca tcatgtaatt aagcggatgc
    14821 atacattttt tacaattgtt cagcctggct ttgaacagat tgcattcaat gagctgttac
    14881 atatgggcat caaaccgctg gctattacaa aaggcgggat agtattccgc gggaatcttt
    14941 acgatagcta taaagcaaac atgtttagcc gtgttgctac acgtattatc atgcgtttaa
    15001 cagaattcag ggctctgcac tttaatgaga tttaccgcac actgagaaat tttccatggg
    15061 aattatatat aagcagtggg acatctattg ctattgaagt atcggttcat aaatcaaaac
    15121 ttcatcatac cgatagaatt ggtaaagaag ttcttaacgc cattgaaaag aggatgaatg
    15181 attttgcact gcatataaat actgaccaat accgggtaca aacaatttat atacggtttg
    15241 atcatgacca gtgtggtgtc agtatggata caaccggtga tcctttatat aagagaggat
    15301 ataaacagca cgttgggcgg gcaccattac gagaaacaat agcagctgca ttgctgtatg
    15361 aatgcgatat aaccagctat gatgttatca ttgatcctat gtgcggtagc ggaacatttc
    15421 cactcgaagc atggtgtatg acaaacaatt tttttccagg gataaaccgt gcgtttgcct
    15481 ttatgcactg gccatcattc agaaaagagg catgcaattc attggcatca tcattgagca
    15541 ggggaaacta tggcgcaaca atacttgccg gcgacaggga cccatcaatg gttactatcg
    15601 ccaaaaacaa ttgtactata cttggtgctg caataactac ctatagtggt gattttctta
    15661 ctgaaaaatt aacgattgaa gggaaacata tcttatgtac aatgaacccg ccttatggca
    15721 agcgcatagc tgatgatgac acagcaggaa tatatagcag gctgtcatat atttttgaaa
    15781 actggtatcc tgcatggagt ttttgtatta tcattccttt atcactgcgg catgtcttca
    15841 ccgtccctgt aacgaaagag atacgattta acaatggtgg attgcctgtc attgctctga
    15901 ttggaaggga gcgatagctc tccacaattt gcttagcttc tgagaaaaaa atatattaaa
    15961 agctataatc ttatctttga ggacacatgc ataatcatgg agtgacacca tcttatcggc
    16021 aagggtgaca acccacgatt caccatagcg aggtggtatg ggggttagtg gccacatatg
    16081 tttaaggata atatcttttt ctttttttga tataacaaaa tgttttgcag cattgttaag
    16141 cgctatgtgt ggatggcgta aaccatgtaa gcgcggaccg ccggtacgcc agttgtacat
    16201 ataaaaatca tgcaataatg caccccgtgt tgcagcgata taatccagat gcagcagacg
    16261 tgaccattgg tacgctaatt gtgcaacagc tatggtatgc tgatagcagc tgattgtgcc
    16321 gtgatgagca aaaattttta aggtattaaa tgcaggatgc aacagtatat cctgcacaaa
    16381 gggtaatgac tcaaatcgta tggtttccat agttatttat gtttattatg attatcaatc
    16441 atttttgtta tgctttgtag gaacatggtg tatcgttctt tgatgctgct taccagacgc
    16501 aatgtttttg gtggtatcat tttatttatt tttttccgat acgagatggc attatcaaca
    16561 aaatatcgta atgtcagaag agcatcattc tgaaaacgta tagccattgt atgctgcaag
    16621 aaggatggag ttttttttgc attaataagc ttttgaacaa cactatgtgt cagcaagcaa
    16681 tctattatca taattgttaa gcccgaaaaa gctatagtat ttaccagtgt ttcaggtata
    16741 gtggtataaa aatgtataaa catcgggagc agtacatatt caaatgccag tgcaagcaac
    16801 ccccagataa ttgaaaacgg taaacataca aatccctgta tattcagtgg ttcatctaaa
    16861 taattccagt aacgcttgcc aaacagaata tacagtaaca cccctgatac atattccagc
    16921 acgcttagtg ctaaaaaata aataatacat ttatagataa taggcatgga aatagtagca
    16981 taggcgatag ttgttataat aactgcacct atcccgtaaa tgggaagata tggtcctgtt
    17041 aaaaaacccg gatttacaat ttttttatta ttgagagaac gatagataac ttctaatatc
    17101 caccctgctg tagaaaagat gataaactca aacagcatca gctggtaatc atgcattggt
    17161 tttgtctcca tgtattggat tagatttaaa tattgaactg gaaataaaat cgatacttga
    17221 ctctaaagaa tcaagggaat aggtgatact gttttttaat gttgcttcaa tacctttaag
    17281 aaaaagctgc attgtgttga taatctgctg actatttgca ttatgtaata accctgcttt
    17341 tgaagcttca gaaatgatat tttgtaacat atgctgttca cttgcattga gttcttcaga
    17401 aaaatcctca tcgtggagtg aagagtcttt aataaatgga ttgttaataa aaagctggat
    17461 atgctccctg atcaatgttt tcattttttc aatgggttga gtgtttgcag taaggagttc
    17521 ctgtaatttt tgatcgaatt gctgtatctt ttccagtatc aacgctttga gcaatccctc
    17581 tttactgcca aaatagttat agatagttgc cttggcaacc cgtgcatcat gggcaatatc
    17641 atcaacggct gtttttttta caccaaacct gttgaacatc atctcagccg tgcgaagtat
    17701 ctggtgttgt tttcgtttta tcatggtact accattttat acttttatac tattttagta
    17761 taaaataata ctaaaccagg ctatcgtcaa caataaaata aaaatttttt tagcatcatg
    17821 ttgtagatat atcaaatgag agacaacccg aggtctatca tgacatcaag gatatgctgc
    17881 tggatgacat tatctggtta atctggttgt tgggagggat ttataattaa tgtgatgaac
    17941 aattattgac agctaaataa tgatattttg tgaggtacta ctaatcaaaa tataaagatt
    18001 aacattcaca aaaatatacc aagcgttgta ttaaaatctg ttaaattata caggttttgt
    18061 tcatttttaa aaaatgcttg tatttttcaa ggtgattatt caaatggtca tattcatagt
    18121 aatacaataa aaattagttt tagtttaatt taaaatgtta aatggaattt aaatggctgc
    18181 aatcaaaaga gcattgataa gtttgtccga taagtcgggg ataataccgt ttgctaaaaa
    18241 gctcaataat ggtggtgttg caattttgtc tacaggaggc actgccaggc ttctcaggga
    18301 taatggtatt gatgctactg aagtttcaga ttacaccggg tttccggaaa tgatggatgg
    18361 gagggtaaag acgctccatc caaaaataca cggtgcgcta ttgggattgc gtgataatcc
    18421 tgaacatata gaagcaatgc agaagcatgg tataataccc attgatatgg tggtgattaa
    18481 tctataccca tttcaacaga caatttccag aggatgtacc cttgacgaag caattgaaaa
    18541 tattgatatt ggcggacctg caatgcttag atcagctgct aaaaattata aatatgtaac
    18601 cgttattgtt gaccctgaag attatgatac tgtcttaagc gagatggaat tgaatgatgg
    18661 atgtgtgtca gaagaaacaa attttaggct ggcaagcaaa gtttttgagt tgacatcaca
    18721 gtacgattca atgattgcta catatttaaa agaacaaatt aagaatgctt aaagggtaaa
    18781 ccgttgcaag cggagcccat aatgtgcata ttacaatata aacatcggag gtaatagatg
    18841 gcagaagtta cattaaaaga gatttacccg gttccagaga aatt
//
LOCUS       DAOOJH010000039        18866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2892_length_18866_cov_10.011695, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000039 DAOOJH010000000
VERSION     DAOOJH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2892_length_18866_cov_10.011695"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PLJ75_06835"
     CDS             complement(<1..1030)
                     /locus_tag="PLJ75_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPD04968.1"
                     /translation="MNLFNKDTYVPLAERMRPQTIEEFVGQRHLLSKGKLLYSVFDQK
                     KALSIILWGDPGTGKTTLARLIAHTCRMDSYFISAISAGVADVRKVIEKGRDNRSQGI
                     QTLLFIDEIHRFNKAQQDAVLGAVESGDIVLIGATTENPSFSIISPLLSRTRVLRLYP
                     LQEDDIRVIVTRALTHDQELSHKNISIPDEALDIISQLAMGDARRALNILEAAVFLKG
                     EGQLNKEIVLEAYREQSIAYDKSGDKHYDTISAFIKSLRGSDPNAAIYYLARMLVAGE
                     EPEFIARRMVIFASEDIGNAYPLALTVATSGMIAVKNIGLPECEIILAHMATFLASCP
                     KSNASYMAL"
     gene            complement(1033..>2132)
                     /locus_tag="PLJ75_06840"
     CDS             complement(1033..>2132)
                     /locus_tag="PLJ75_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPD04969.1"
                     /translation="GTVQADILKEEQAQNTCIFSIDFALKLMGDMQEYFIKHTINNFY
                     SVSISGYHIAEAGANPVTQLALTLANGFTYVEYYLARGLAIDDFAPNLSFFFSNGLDP
                     EYSVIGRVARRIWAKAMKYKYGGNERSQMLKYHIQTSGRSLHSQEIEFNDIRTTLQAL
                     YAIYDNCNSLHTNSYDEAITTPTEESVRRALAIQMIINQEYGLAKNQNPLQGAFIIEE
                     LTDLVEEAVIAEFNNISIRGGVLGAMETMYQRNKIQEESLYYENKKMTGELPIIGVNT
                     FLSKDGSPIQIPNEVVRATPEEKDVQLESLAQFHRVWKPEADIALQKLKQIVKQHENV
                     FETLMDVAQYCSLGQLTDALYSVGGMYRRSM"
     assembly_gap    2133..2232
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2233..4286)
                     /locus_tag="PLJ75_06845"
     CDS             complement(<2233..4286)
                     /locus_tag="PLJ75_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPD04970.1"
                     /translation="MLHASTVQKYIPKNHIRIVTAASLFDGHDAAINIMRRIMISTGA
                     EVIHLGHNRSVDEIVKSAIEEDVQAIAITSYQGGHVEFLKYMYDLLKNRNADSIKIFA
                     GGGGTILPDEIKDLEAYGIAKIYSPDDGRAMGLQGMINDLLSKCDYPEGNIDSIPIDD
                     IKNMDIRAIARAISACENFRENIKNDLLTVKKLIKENDTPVLGITGTGGSGKSSLVDE
                     IVLRFLQSNPQKTIAIVSVDPTRQKTGGALLGDRIRMNAINHANIYMRSLATRGSKSS
                     VSHYIQDAIDILKLAGFDVIMLETSGIGQAQTDVLDYCNCSIYVMTPEYGAATQLEKI
                     DMLDYADIVVINKFDKSGALDALHDVKKQMQRNKEAFDMPLEQMPVFGTIASQFNDDG
                     VNKFFNYLIEKLNEKLNEKGGKKFAAYVAEKQKKDIVYIVPPERTRYLSEIAETIKKY
                     NNNTLEESKKALYLYSIKKCIEYYKQSKSEHSVIDELNKKYHEVYGSLSADSKTILKT
                     WEDKKKNYQGEYYTYHVRNKEIKVKAYTESLAHTKIPKVCVPKFENWDDIITWNMQEN
                     FPGQFPYTAGVYPFKREDENPTRMFAGEGIAEKTNKRFHYLSYHMPAVRLSTAFDSVT
                     LYGYNPDKRPDIYGKIGNSGVSISTIDDVKRLYSGFKLTDPSTSVSMTINGPAPIMAA
                     YF"
     gene            4459..4596
                     /locus_tag="PLJ75_06850"
     CDS             4459..4596
                     /locus_tag="PLJ75_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04971.1"
                     /translation="MPQISLYIDKDTHAKIEKAAAKERISISKWIGKNIKKVIKEDYP
                     Q"
     gene            complement(5102..5368)
                     /gene="rpsU"
                     /locus_tag="PLJ75_06855"
     CDS             complement(5102..5368)
                     /gene="rpsU"
                     /locus_tag="PLJ75_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPD04972.1"
                     /translation="MPVPLEVEVGDGDLERAFKNLKKRMAFEGIFKELKRRRYYEKPS
                     EEKKRKKEEAERRRLKKVRRFETQSKQRRFVAKPSGRGGSSQDE"
     gene            5617..6105
                     /locus_tag="PLJ75_06860"
     CDS             5617..6105
                     /locus_tag="PLJ75_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04973.1"
                     /translation="MKNCKITILIALFLFVSLYCCSKPVQKSLIPSQPQSPDFNITTI
                     VLECSQKHSNLLPFVELVLLLHNDNTSRYFIATVYGTMYATYTTPPSFAPSHAVCGVT
                     VNHNTLCHYFFVCVTNDAVIIQSTEKKCFDEPDSEKYHTIITIPWKHVNTIQTKVIIN
                     DR"
     gene            6219..7157
                     /locus_tag="PLJ75_06865"
     CDS             6219..7157
                     /locus_tag="PLJ75_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPD04974.1"
                     /translation="MIIGLDIGGTNIKGVIINNDGTILAKTSVITGAGIDATENNIGN
                     CIKTLLELSHKNKNEVDAVGIGSAGAIDKQNGMVITSPNIPAWKHYPIVQRVEKLTGY
                     TVFLENDANAAVVGELWLGNGKNYKNWIMLTLGTGIGGGAVINGMPYTGNNGSAMEFG
                     HITIDYNGLQCKCGRKGCLECYGSATAVVHFVKSHCTFYPDSSLNTISPHAMTARHVY
                     DEAKKGDPLALYVFNLIGTYLGIGIADLVNIFNPEAVILGGGLSSASQYFLPHIIHQV
                     QKLSLEGMKENIRYMITKHGDYTAAIGAAKHALETI"
     gene            7170..8069
                     /locus_tag="PLJ75_06870"
     CDS             7170..8069
                     /locus_tag="PLJ75_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPD04975.1"
                     /translation="MFFSNQLLFSEHTLNQIHIYAGRWLDFLMVAFTHCGDEIFYILL
                     IPFLFWCVNKRLATIVGLSFLFSTVFNDIIKFAFNKPRPNPLNLAEGIAQLNNVYCPP
                     SSPGFPSGHAQNAVVFWGLLAYTIRRRLFSIFAVFMIVAISYSRLYLGVHFFGDVAGG
                     IIIGCMVFTCAVLLIPYFDRLWNLQLLSAIIIFVMMPVAVAIAFPDDATVKSLGVFSG
                     FSIGLIVTKNRFGEPSFKLVPALVNYFIGITVLLLLKEGIKVILPSVAYADFIRYWII
                     GGWISFGAPLTFNHIPYLSKIYR"
     gene            8313..9350
                     /gene="pheS"
                     /locus_tag="PLJ75_06875"
     CDS             8313..9350
                     /gene="pheS"
                     /locus_tag="PLJ75_06875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPD04976.1"
                     /translation="MYMKQQLENLYKQTIATIEKAVSIDELEQIRVKVLGRKGELTQI
                     LRSLGTLPQEERIETGKLSNELKDSIEEALEKRKTQLTQQHINKLIMEDWLDVTLTKP
                     GLLQEYQRGHLHPISQIQYEIEDIFTSMGFEILDGPEVETDYYNFEALNIPPYHPARD
                     MQDTFWTEDGNLLRTHTSAIQIRGMERLKPPFRVIGPGRVFRYESIDASHENTFYQVE
                     GMMIDKDISVAHLIYFMKTLLKEIFKRDITIRLRPGYFPFVEPGFELDIQCLICGGKG
                     CSVCKYSGWLELLPCGLVQPNVLRYGKIDPDKWSGFAFGLGLNRLVMMRYGINDIRHF
                     LSGDIRFLYQF"
     gene            9385..11781
                     /gene="pheT"
                     /locus_tag="PLJ75_06880"
     CDS             9385..11781
                     /gene="pheT"
                     /locus_tag="PLJ75_06880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPD04977.1"
                     /translation="MWISLNIIQDMVDIKDIPVSEIANRLTMSTAEIDSIEHVNQLLN
                     TIYTAKILEVSKHPNADKLTVCKVDTGSNVIQVVCGAPNHKAGDIVALAPAGTKFSDT
                     ITITKTRLRGVESEGMLCSERELGLSDDHSGIMIFPPDTPIGVSLASLFPHKIDVRLE
                     IDNKSITHRPDLWSHTGFAREIAALFKRSFKYPVKYDVAKECKATNSLVVTIINPEGA
                     PRYCGLVVDNITIKESPDWLKARVEAIGMRPINNIVDVTNYVMAELGEPMHAFDRKKL
                     RGNEIIVRFASEGEHLMTLDGQEHILTSQDVVIADSGGPIALAGVMGGGNSEIEDNTT
                     SIVLEAANFNPVYIRRTANRHNLRTEAAIRFEKSLDPEVCPDAILRCYELIKMLLPEA
                     EATTHIVDAYPVKAKPISITTTTDFIRRKLGHAIDDDTIVSILTSLDFKVNKQDNNLT
                     IDVPSYRATKDVSIPDDIVEEIGRIYGYDNIPQQAPLVPCAPPQQNIKRTFERTVKSI
                     LVNDYHMTEVSNYSFVGEALLNRLQMNEDTGLRLKNPLSKEQDRLRESLIPNIILNIT
                     TNQRFFDDFRIFELGRVYHKQDRTSPDLAMENTRVAGIVYAKKPLYPLFYDAKECCMG
                     LFHKTKRTNYSINPACNQLPPYAHPARSLEIAIDNSPIALICELHPKIINEFEIKGSV
                     ALFDVDIDLLLNSPVQETIFKELPRFPDVPFDISILADEKEYAATLVTMLYKADTTHI
                     KNVEVISVYNQPPIPQGKKSVSFRIIFRADDHTLTPEEADSLQNKVIRFVEKNGYKLR
                     "
     gene            complement(11778..12479)
                     /locus_tag="PLJ75_06885"
     CDS             complement(11778..12479)
                     /locus_tag="PLJ75_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04978.1"
                     /translation="MKKIVGGVIAILLILFGFMMYISHKVIRINDMDIPDFQPLKDNT
                     LAEKYCPYIIANHEYEYPYAVYYRASIDNNGNTHIAYHFFWEREVNKTRGFVPWLTRN
                     LYTGGIKLQEIMFGKHDIEVIGVVLDKNHNIKKVIYESPEHYNPYDFIVKHKTNEIAD
                     TVHTPLRFKVVSWNHLFAFVDNNYNLKKGEVELYRKPQYFTKKLWDEFTMCKKEETAV
                     KRNRAHYAWEREYID"
     gene            complement(12560..15265)
                     /locus_tag="PLJ75_06890"
     CDS             complement(12560..15265)
                     /locus_tag="PLJ75_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HPD04979.1"
                     /translation="MMNRKLLIALTIFITGYILVYSIGKFYIDYRWFSIYNHLDIFWI
                     LFFSKFNVQTIFWGLFIALFILNFILIRILGGKGRIFTKNILDKIRIPGFGTTRNLLF
                     IILIIGVVVLGFFMGLWASTYWKEYLMFKNEVAFTDFPKEPLFNMDIGFFVFSLPFYK
                     FIFRWVLVSLSFITIFSFFFHILNNGIFSQNGKLEFSRFARAHLSILFGLIVLIIASG
                     YRLLAYDLLFNNRAKFFGAGYADVHAKLLAYDICMVISIIAAILLFANMVIRSFKMPI
                     ILLITLLPVYFVFGTIFPALQQRFIVDPNELEKEKPYIQYNINYTRIAFGLNGVKELP
                     FENAQTLTFRDLEKNRDVVNNIRLWDWRPLKETYKQLQGLKPYYDFIDVDIVRYTINN
                     QKVALNISARELDNTKLTVSKNSWLNNHLIFTHGYGVIASRVDKITPEGLPELVLYDI
                     PTKSTIPIPVTVPQIYYGEHQNSYIITNTKITPGEFDYPYGDENKYTIYNGTGGDVLN
                     SLVKRAMYAIAFGDFNILISNVITNQSRIHFRRNIVDMVTAITPFIYYDSDPYVVVVD
                     GKIYWIIDGYTYSSYFPYSTPIQLTNGVSINYIRNPIKIIIDAYNGTIRYYIADNNDP
                     VIKAYAKIFKNLFIPMEQMPDAFKDHLRYPEALFNIQAQQLLRYHMTNINVFYNNEDL
                     WDIPQQIYESAREKMHSYYLVTSLPNEPATEFLLILPFTPAKKDNMIAFMVARCDIPN
                     YGELRLYTLPKDKLNFGPMQIEARINQDAEISKQLSLWNQRGSRVIRGNMLVLPIRDS
                     ILFVEPLYLKAETSEMPELKRVIVSYKDKIIMEENLSLALSKLFTGGSFGERYYGGYA
                     IKDLVNKAYMHFIQAEEYQRQGNWAKYGEELKNLKETLTALRNAR"
     gene            complement(15282..16613)
                     /locus_tag="PLJ75_06895"
     CDS             complement(15282..16613)
                     /locus_tag="PLJ75_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD04980.1"
                     /translation="MKQKNGFAKKELFAVILIALMNLFLFADQNLMAPNLTQIAHDFG
                     FNDVQRDVMLGGHISFVFWILGGLVTLAIGYLTDKISRRNLFLFVIIVGELPCLLTGF
                     AQNYDQLFWLRALTGIGIGGALPLTFSIIGDYFSHENRAAASAWIGLAQGLGIAVGQL
                     MAGFIGSAYGWRLPFIIVAIPNFILAFIFLLFVKEPQRGKTEESLKELIEEGKVYTGK
                     INWREYKSLFKIKTNILVFLQGIPGTVPWGVFFIFLNDFYSQDKGYSIEVATLIVLTV
                     GAAAILGAFIGGFIGNWLYNIKPKYLPMLCGTSTLLGIIPMALLLNYTPQVIIPEPDP
                     TLPLIYGFITGFTVTITGPNVRAILLNVNTPETRGSIFSLFNLTDDLGKGFGPVIISA
                     LIVLFGRLMAFNIANLFWLLCGLLLLVMIKTFPEDEARLNAILKEKAEKMK"
     gene            complement(16841..17014)
                     /locus_tag="PLJ75_06900"
     CDS             complement(16841..17014)
                     /locus_tag="PLJ75_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04981.1"
                     /translation="MYYSGIDPSWSGKYPTVVAVSNNSLKVVQYIYTKRVNDIICAIA
                     PYNNSMIEIDALV"
     gene            complement(17107..18777)
                     /locus_tag="PLJ75_06905"
     CDS             complement(17107..18777)
                     /locus_tag="PLJ75_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD04982.1"
                     /translation="MEKIWLKSYAPGVPHTLKYDEITVVHGFERSAKDFPNKNAMIFL
                     DAKISFKKMQSMVNKIANALIDLGVKPGDKVAMLMPNMPQIVIGSYAAWKIGAVVVMN
                     NPLYTDHELEHQLNNSESTVLITLDLLGPRMIALRPKTQVKKIIVAHIRDHLGFPKKQ
                     LLPIVAKANHRNIPPTPEVYEWTDLLKKYPDKDPGTMAKFNDLATLQYTGGTTAVSKG
                     AMLSHKNLSSNVQQIKAWFPEFKRGELTSIGVLPYFHSFGMTTVMNISAFYGWTQVLI
                     PRPEAGAVLEALIKHKVDFMPAVPTFYVGLLNHPKIKEADISTIKGCFSGAAPLPVDV
                     IREFEAKTGAPICEGYGLSETTPVVTINPYGGKTIPGSIGLPIPDTECKIVDLDEGTK
                     EMEIGQEGELCIRGPQVMSGYYKMPDETAKTLRDGWLYTGDIAKVDENGYFYIVDRKK
                     DMIIAGGYNIYPRDIDEVLFEHPKILEACAIGVPDKYRGETVKAFVVLKPGETMTEQE
                     VIDYCKTKLAAYKVPKMVEFINELPKSNVGKILRKELRKMELEKMEKK"
BASE COUNT         5815 a         3711 c         3186 g         6054 t
ORIGIN      
        1 gcagtgccat atacgatgca ttgcttttgg gacacgatgc cagaaaagtt gccatgtgtg
       61 ccagtattat ctcgcactcg ggcagtccta tattctttac cgcaatcatc ccggatgttg
      121 ccaccgttaa tgcaagagga tacgcattgc caatatcctc cgaagcaaat atcaccattc
      181 ggcgtgcaat aaactcgggt tcttcaccgg caacaagcat cctcgctaaa taatagatag
      241 ctgcattggg gtcagagcct cgcagtgatt ttataaatgc tgatattgta tcataatgct
      301 tatcaccgct tttgtcataa gcaatgctct gctcccggta tgcctccagt acaatctctt
      361 tattgagttg cccctcacct tttaaaaata ccgctgcttc aagtatgttc aatgctcgcc
      421 gcgcatctcc cattgccaat tgggatataa tgtccagtgc ttcatcaggt atacttatat
      481 ttttgtgcga tagttcctga tcatgagtca atgcccgggt aactatcacc cttatatcgt
      541 cctcctgcaa aggatacagc cgtaaaacac gggttcgcga caataacggc gaaataatgc
      601 tgaacgaagg attttcagtg gttgctccta ttaaaacgat atcaccactt tcaacagcac
      661 caagcactgc atcctgttga gctttgttga agcggtgaat ctcatcaata aaaagcaatg
      721 tttgaatgcc ctgtgaacgg ttatcccttc ctttttctat aaccttgcgc acatcagcaa
      781 cgcctgccga aatagcgctt ataaaatacg aatccatccg gcatgtatgc gcaataagcc
      841 ttgcaagggt agttttgccc gttcccgggt caccccacag gataatcgac agagctttct
      901 tctgatcaaa gacagaatac aacagtttgc cttttgaaag caggtgcctc tgaccaacaa
      961 attcctcaat tgtttgcggg cgcatccgtt cggctaaggg aacataggtg tctttgttaa
     1021 atagattcat tattacatgc ttcttctata cataccacca acgctgtaga gtgcatcagt
     1081 tagctgtcct aaagagcaat attgagcaac atccattaat gtttcaaaca cattttcatg
     1141 ttgttttact atttgtttta atttttgtaa ggctatatcg gcttccggct tccataccct
     1201 gtgaaattgc gctaatgatt caagctgtac atctttttct tccggcgttg ctcgtacaac
     1261 ctcattggga atctgtatgg gtgagccatc ctttgaaaga aaggtgttta ctcctataat
     1321 gggcaattca ccggtcattt ttttattttc atagtaaagt gattcttcct gaattttatt
     1381 gcgctgatac atggtttcca tggctcctaa aacgccaccg cgtatactga tattgttaaa
     1441 ctcagcaata acagcctcct ccaccagatc agtcaactct tctatgatga atgctccctg
     1501 cagtgggttc tgattttttg ccagtccata ttcctggtta ataatcatct gtattgctaa
     1561 tgcccggcgc accgattctt ccgtaggcgt ggttattgct tcatcatatg aattggtatg
     1621 caaagaatta caattatcat aaatggcata caaagcctga agggtggtac gtatatcgtt
     1681 aaattcaatc tcctgtgaat gaagcgatct ccccgaagtt tgtatatgat atttaagcat
     1741 ctgtgaacgt tcattaccac catacttata tttcattgcc tttgcccata tcctgcgcgc
     1801 aacccgccct ataacagaat attcaggatc aagtccattt gaaaagaaaa atgacaaatt
     1861 gggggcaaaa tcgtcgatgg caagccctcg cgccagatag tattcaacat aggtaaaccc
     1921 attagctaac gtcaatgcca actgcgtaac aggattggca ccagcttcag cgatatgata
     1981 ccctgaaata gataccgaat aaaaattatt aatggtatgc tttataaaat attcctgcat
     2041 atcccccatc agtttcaatg caaaatcaat tgaaaaaata catgtattct gagcctgttc
     2101 ctctttcaat atatctgcct gcacagtgcc ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnaaaaagta agctgccatt attggagcag gaccattgat ggtcattgaa
     2281 actgatgtgc tgggatcggt aagcttaaac cctgaatata acctttttac atcatcgata
     2341 gttgatatcg aaacacctga attgccaatc tttccataaa tgtcaggtcg tttatcagga
     2401 ttataaccat acaatgtaac agaatcaaat gcagttgata accgcacagc aggcatatgg
     2461 tatgaaagat aatgaaaacg tttatttgtt ttttctgcta tgccttcacc tgcaaacata
     2521 cgggtaggat tttcatcttc acgtttaaat ggatatacac cggcggtata cgggaattgg
     2581 ccggggaaat tctcctgcat attccatgta ataatatcgt cccagttttc aaattttgga
     2641 acacaaactt tgggaatctt tgtatgtgcc aatgattcgg tatatgcttt tacttttatt
     2701 tctttatttc gtacatgata tgtataatat tcaccctgat aatttttttt cttatcctcc
     2761 catgttttta aaattgtttt gctgtcagcg ctaagtgatc catatacctc atgatacttt
     2821 ttattaagtt catcaattac tgaatgttca ctcttacttt gtttgtaata ttcaatacat
     2881 ttttttatgc tataaagata aagggctttt tttgattctt ccagagtgtt attattatat
     2941 ttttttattg tttctgctat ttcagaaaga tatctggttc tttcaggcgg tacaatatat
     3001 actatatcct tcttttgttt ttcggcaaca tacgcagcaa atttttttcc acctttttca
     3061 ttcaattttt catttaattt ttcaatgaga taattaaaaa atttattgac accatcatcg
     3121 ttaaactgac tggcaatagt tccaaatacc ggcatctgct caagcggcat atcaaaagct
     3181 tctttattgc gctgcatctg tttttttaca tcatgcaacg catcaagtgc tcctgattta
     3241 tcaaatttat ttataactac tatgtcggca tagtccagca tatcaatttt ttccaattgt
     3301 gtagcagctc catattcagg ggtcatgaca taaattgaac aattacaata atccagaaca
     3361 tctgtctgcg cctgcccaat tccagaggtt tcaagcataa tgacatcaaa accggctaac
     3421 tttaaaatat ctatcgcatc ctgaatatag tgagacacag aagactttga gcctcgagta
     3481 gccagtgacc tcatatagat attggcatgg ttgatggcat tcatccgtat gcggtcgccc
     3541 aataaggcac cacctgtttt ttgtcgtgtg ggatcaactg atactatagc aattgttttt
     3601 tggggatttg actgcaaaaa ccgcagtact atctcatcaa caagcgaaga cttacctgaa
     3661 ccacccgttc cggtgatacc taacacaggc gtgtcatttt cttttattaa tttctttaca
     3721 gtcaataaat cattcttgat attttccctg aaattttcac atgccgatat agcacgggct
     3781 attgccctga tgtccatatt ttttatatca tctattggaa tggaatcaat attaccttca
     3841 ggataatcac attttgataa aagatcatta atcatgccct gtaaacccat tgctcttcca
     3901 tcgtcgggcg aatatatctt agcaatacca tacgcttcaa gatcctttat ttcatccggt
     3961 aatatcgttc caccacctcc agcaaaaatt tttatggaat cggcattgcg atttttcaat
     4021 agatcataca tatatttaag aaattccaca tgaccaccct gatatgaggt aatcgctata
     4081 gcctgtacat cctcctcaat tgcacttttt actatttcat ctactgaacg gttatgccct
     4141 aaatgaataa cctcagcacc ggtgctaatc atgatgcggc gcataatgtt aatggctgca
     4201 tcatgcccat caaataatga tgctgcggtg actatccgga tatgattttt tggaatatac
     4261 ttttgaacag tactcgcatg taacatataa catcaacccc cctatctatt ttttggtcat
     4321 attattttat atattacaat attacaatat tttttttcat attagcgaca aatatattgg
     4381 tgcatgaaaa aatattacta tagctaatat acttaacttt tatctattat cctattatcg
     4441 ttgtcatagg ggtttcctat gccacaaata tcattatata tcgacaaaga tactcatgcg
     4501 aagatagaaa aggctgcagc caaagaacga atttcaatct cgaaatggat aggtaaaaat
     4561 attaaaaagg tcataaaaga agattatcca caatgatatt ttgatttatt cggttcaata
     4621 acagataaac tggattgtga aataaatgca acagccgaaa acaacgctta tgcgcatcgt
     4681 cgctgcgctt ctcggcctcc gcgccaaaaa tatgaagacg ggcgctacgg cacattttca
     4741 cctccgttat cgctacggtg aaaacgtcat atacgctgga tacgttatta tgcaagcagc
     4801 atgttaaact ctttactcac ctttgaaaaa actgctgttt ctgtaaagtt tttgtcatta
     4861 ctgcaaatgc agaaatctaa tacatcaaaa ttagatttgt ttgtcattat gtacatataa
     4921 tatttattca ttatttgttg actttatcca tccgcctatc atgtcgcacg cttcattaat
     4981 ttttaacgga tatatgctca cactatttta tggaaaggta ccacgtatga taaagactca
     5041 ataaataaaa aatccggctg aatgccggat tttcctgttg ttttcattat cacagttaaa
     5101 actattcgtc ctgtgatgaa ccacccctgc cagaaggttt tgcaacaaac ctgcgttgct
     5161 ttgattgagt ttcgaatctt ctgacttttt tcaggcgccg ccgttctgct tcttcctttt
     5221 tgcgcttctt ttcttcgctt ggcttttcgt aataacgcct gcgtttaagc tctttaaaaa
     5281 tcccttcaaa tgccattcgt tttttcagat ttttaaatgc acgctcaaga tcgccatctc
     5341 ctacctcaac ttctaaaggt actggcatat tgtaatcaca tcccttctta attgttttac
     5401 attattttaa tgtaattatt atattgtagc tctatcacgt ttacattact gaaaatactg
     5461 tacttttgtc aacataaaaa attatatctc tccttttgcc attagtttta acaaatattc
     5521 atgaaaacgg ttgacaatac ataaaaatat gctctttaat agtacaccat gcataataag
     5581 gaatttgata ctttgtttta tttaatagct gtatatgtga aaaattgtaa gattactata
     5641 ttaatagcat tatttttatt tgtatcgtta tactgttgca gtaaacctgt tcagaagtca
     5701 ctcatcccat cacaaccgca atcgcctgat tttaacataa ccactattgt tttagaatgc
     5761 tcgcaaaaac atagcaattt gttgcctttt gtggaattgg tacttttgct gcacaatgac
     5821 aacaccagtc gttattttat tgcaacagta tatggaacta tgtatgccac ttatactacc
     5881 cctccatcct ttgcaccttc acatgcagta tgtggagtta ccgtaaacca taacacactg
     5941 tgccactatt tttttgtatg cgtaaccaac gatgctgtta ttattcaatc aacagaaaaa
     6001 aaatgttttg atgaacctga tagtgaaaag tatcatacta ttatcaccat accatggaag
     6061 catgtaaaca ccatacaaac taaggttatt ataaatgaca ggtaatgaaa taaattttca
     6121 atggttattg ccatgaataa taaaaaatta ttgattacct gctggttgca atcattatat
     6181 ggctatcttt caataaatag taatgcagga attttttcat gattattgga ctggacattg
     6241 gcggaacaaa tatcaaaggc gtcatcatca acaatgatgg cactatactg gcaaaaacat
     6301 cagtcataac aggagcaggt attgatgcca ctgaaaataa tattggtaac tgtatcaaaa
     6361 cattactgga actatcgcac aaaaataaaa acgaggttga tgctgtaggc attggttctg
     6421 ccggtgctat tgataaacaa aacggtatgg taatcaccag cccaaatatt cctgcatgga
     6481 agcactatcc aattgtacag cgtgtggaaa agcttaccgg ttatacagtg ttccttgaaa
     6541 atgatgccaa tgctgcagta gttggcgagc tatggcttgg caacggcaaa aattataaaa
     6601 actggattat gcttaccctt ggcaccggca tagggggcgg tgctgttatc aatggtatgc
     6661 cctataccgg caataacggg agcgctatgg aatttggaca cataactatt gattataacg
     6721 gcctgcaatg caaatgcggc agaaagggat gtctggaatg ttacggctcg gcaacagcgg
     6781 ttgttcattt tgttaagagt cattgtacat tctatcctga ttcttcactc aataccatat
     6841 caccacatgc gatgaccgca cgccatgtct atgatgaagc aaaaaaaggt gatccgcttg
     6901 ctctgtatgt ttttaatctc attggaacat atttgggaat tggcattgct gatctggtaa
     6961 atatatttaa tcctgaagca gtgatactgg gcggaggatt atccagcgcc agtcagtact
     7021 ttcttccgca cattattcat caggttcaaa agctttccct tgaagggatg aaggaaaaca
     7081 tacgctatat gattaccaaa catggagatt atacagcagc cattggcgca gcaaaacatg
     7141 cattagaaac tatataacag gaggatgtta tgtttttcag caatcagctt ttattcagcg
     7201 aacatacgct caatcagatt catatctatg ctggcagatg gcttgatttt ttaatggttg
     7261 cctttaccca ttgcggcgat gagatatttt acatcctgtt aataccgttt cttttctggt
     7321 gtgtcaacaa acggcttgct acaatagttg gattgtcatt tttgttttcc actgttttta
     7381 acgatattat taaatttgct tttaataagc cgcggccaaa tcctttgaac ttagccgaag
     7441 gtattgccca gcttaacaat gtatactgcc ctccatcaag tcctggattt ccaagcggac
     7501 atgcacaaaa cgcagtggtt ttctggggtt tgctggcata tacaatcagg cgcaggttat
     7561 tttccatctt tgcagttttc atgatagttg caatttcgta ttcacggctt taccttgggg
     7621 ttcatttttt tggcgatgta gcgggtggta tcatcatcgg ctgcatggtg tttacatgcg
     7681 ccgtgctgct tatcccctat tttgacagat tgtggaatct acaattacta tctgccatta
     7741 ttatttttgt catgatgcct gttgcagtag ccattgcatt ccccgatgac gcaacggtta
     7801 aatcgctcgg tgttttttca ggattttcta tcggcttaat tgtcactaaa aatcgttttg
     7861 gcgaaccatc atttaaactt gtgcctgcac tggttaacta tttcattggg ataaccgtat
     7921 tgcttctttt aaaagagggc atcaaggtca ttttaccttc tgtggcatat gcagatttta
     7981 ttcgctactg gataataggc ggctggattt cgtttggtgc gccattaact ttcaatcata
     8041 tcccctattt atctaaaatt tacaggtaat gttgttgact ttttataaac agttgatttg
     8101 taatacactt ctaaaattta ttttgacagt aactatcacc catactcatt tttttggcga
     8161 tagttactcg ttatgtattt tgttttccgg gcattgttga aaattgtttt tttattgtaa
     8221 tattaatgga gcaggcgaat ctaacaccac tgcatgctca tagtagtcac aatgaaatct
     8281 tcaggtgccc attattattt atgtgtcagg tgatatatat gaaacaacaa ctggaaaacc
     8341 tgtataaaca aaccattgct accattgaaa aagctgtctc gatagatgag ctggaacaaa
     8401 tacgcgttaa ggtattggga cgcaaaggcg aattgacaca gattttgcgt tcgctgggta
     8461 cactaccaca ggaagagcgg attgaaacag gaaaactttc aaatgaatta aaagatagca
     8521 ttgaagaagc tctggaaaaa cgtaaaacac aactaacgca acaacacatt aataaactca
     8581 tcatggaaga ctggcttgat gtaacgctga caaaaccagg tttattacag gaatatcagc
     8641 gcgggcactt gcatcctata tcacagatac aatatgaaat tgaagatatc tttacctcaa
     8701 tgggttttga aattcttgat ggtccggagg tggaaaccga ttactataac tttgaagctt
     8761 tgaatattcc gccctatcat ccagcccgtg atatgcaaga cacattctgg acggaagatg
     8821 gtaatttact gcgaacgcat acatcggcta tccaaatacg agggatggaa cgccttaaac
     8881 cgccattcag agttataggg cctggacgcg ttttcaggta tgaaagcatc gatgcatcac
     8941 acgaaaacac attttaccag gttgaaggca tgatgattga taaagatatt tctgtggctc
     9001 atttaatata cttcatgaaa acattgctca aagaaatatt taaacgtgat attaccatac
     9061 ggttgcgccc cggttatttc ccttttgttg aaccgggttt tgaacttgat atacagtgtc
     9121 ttatttgcgg cggcaagggt tgcagtgtat gcaaatattc aggatggctt gagcttttac
     9181 cctgcggact tgttcaaccc aatgttctgc gctatggtaa aattgatcct gataaatggt
     9241 cgggatttgc ctttgggttg ggcttaaacc gtcttgttat gatgcggtat ggcattaatg
     9301 atatacgtca ctttttaagt ggcgatatac gtttcctcta tcaattttaa tgatataatt
     9361 ttataattgt acggagtcgt tactatgtgg atttcactta acattattca ggacatggtt
     9421 gatataaagg atattcctgt tagcgaaata gctaatcgtt taacaatgtc tactgctgaa
     9481 atagattcta ttgaacatgt gaatcaactt ttaaacacta tttataccgc caaaattctg
     9541 gaagtatcaa aacatcccaa tgccgataaa ctaaccgttt gtaaagttga taccggcagc
     9601 aatgttatac aggttgtgtg cggtgcacca aatcataaag caggcgatat tgttgcatta
     9661 gcaccagcag gaacaaagtt ttccgataca atcaccataa caaaaaccag attgcgcggt
     9721 gtagaatccg aagggatgtt atgctctgaa cgtgagcttg gcttatcgga cgatcattca
     9781 ggtataatga tttttccgcc cgacacacct attggcgtaa gtttagccag tctatttccc
     9841 cataagattg atgtgcgtct tgaaattgat aataaatcta taacgcatcg tccggattta
     9901 tggagccata ccggatttgc ccgtgagatt gctgcactat ttaaacgttc atttaagtat
     9961 cctgttaagt atgatgtagc aaaggaatgt aaagctacca attcgcttgt tgtaactatt
    10021 atcaatcccg agggagcccc tcgctattgt ggattggttg tcgataatat tactataaaa
    10081 gaatcacctg actggttaaa agcacgtgtt gaagcaattg gtatgcgtcc catcaacaac
    10141 atcgttgatg ttaccaatta tgtcatggca gaattaggcg aaccaatgca tgcatttgat
    10201 cgtaaaaaac tgagaggtaa tgaaattata gttaggtttg cctctgaagg tgaacatctt
    10261 atgacattag atggacagga acatatctta acatcacagg atgttgttat cgccgatagc
    10321 ggtggtccta ttgccctggc tggtgttatg ggtggtggaa acagcgaaat tgaagacaat
    10381 actacatcga tagttctgga agcggcaaat tttaatcccg tatacattcg aagaacagca
    10441 aaccgccaca accttagaac ggaagctgca atacgttttg aaaaatctct tgatcctgaa
    10501 gtatgccctg atgccatcct ccgttgttat gaacttatca aaatgctttt acctgaagct
    10561 gaagcaacaa cacacattgt tgatgcttat ccggttaagg caaaacccat aagcataaca
    10621 acaactactg attttatacg ccgaaaactt ggtcatgcta ttgatgatga cactatagtt
    10681 tctattctaa catcgcttga tttcaaagtt aataaacagg ataataatct taccattgat
    10741 gtccccagct accgggcaac aaaagatgtt tcaatccctg atgatattgt tgaagagata
    10801 ggcaggatat acggttatga taacatacct caacaggcac cattagttcc ctgtgctcca
    10861 cctcaacaaa atattaaacg gacttttgag cgaactgtta aatcaattct tgtcaatgat
    10921 taccacatga ccgaggtaag caattattct tttgttggtg aagcactgtt aaatagatta
    10981 cagatgaatg aagatacagg attgcgatta aaaaatccat tatcaaaaga gcaggacagg
    11041 ctcagagaaa gccttatacc taatattatc cttaacatta ctaccaatca gcgatttttt
    11101 gatgatttca gaatttttga attgggcaga gtatatcata agcaggacag gacttcacct
    11161 gatttagcca tggaaaatac gcgagttgcc ggtatagtgt atgcaaaaaa accattgtat
    11221 cctttattct atgatgctaa agaatgttgt atgggattat ttcataaaac aaagagaaca
    11281 aattatagca tcaatcctgc ttgtaatcag ctaccgccat acgctcatcc agctcgttca
    11341 ctggagatag caattgataa cagtccaata gcattgatat gtgaactgca tcctaagatc
    11401 attaatgaat ttgaaatcaa ggggagcgtg gcattatttg atgttgatat tgatttatta
    11461 ttgaactcac cggttcagga aactatattt aaagaattac cgcgattccc ggatgtgcca
    11521 tttgatattt caattctggc agatgaaaaa gaatatgctg caacattggt aacaatgtta
    11581 tacaaggctg atacaactca tattaaaaat gttgaggtaa tttcagtata taatcaaccg
    11641 cctataccgc agggtaaaaa atcagtttct ttcaggataa tttttagggc tgacgatcat
    11701 accttaacac cggaagaagc agattccttg caaaacaagg ttatacgttt tgtggagaaa
    11761 aatggatata agctccgtta atctatatat tctcgttccc atgcatagtg agccctgttt
    11821 cgtttcactg ctgtttcttc ttttttacac atggtaaact catcccacag ctttttagta
    11881 aaatactgcg gcttacgata taattcaact tcacctttct tcaaattata attattatct
    11941 acaaatgcaa agagatgatt ccatgaaaca actttgaatc gtaatggtgt atgtacagta
    12001 tctgctattt catttgtttt atgtttaaca ataaaatcat aaggattata atgttcaggg
    12061 ctttcgtata taactttttt gatgttatga tttttatcca aaactacacc tatcacctca
    12121 atgtcatgtt tgccaaacat tatttcctgt aattttattc ctccagtata aagatttctg
    12181 gtaagccatg gaacaaaacc tcttgtttta ttaacctccc tttcccagaa aaaatgatat
    12241 gcgatatgtg tattgccatt gttatcaata ctggcccggt aataaacagc ataaggatat
    12301 tcatattcat ggtttgctat aatataaggg caatattttt cagctaatgt attgtctttt
    12361 aatggctgga aatcagggat atccatatca tttatacgaa ttactttatg agaaatatac
    12421 atcataaatc caaataatat tagcagtatt gctataaccc cgcctactat tttcttcata
    12481 cctaatcccc tgaaataaaa ttattctcca ccgtcattcc gggcttgacc cggaatcaaa
    12541 atatgtaatg acaaaagtat tatctggcat tccttaacgc tgttaatgtt tcttttaaat
    12601 tctttaattc ttcaccatac ttagcccaat ttccctggcg ttggtactcc tcagcctgta
    12661 taaaatgcat atacgcttta ttaactaaat ctttgattgc atacccacca taataccttt
    12721 cgccaaagct accaccagta aatagctttg ataaagccaa tgataaattc tcttccataa
    12781 ttattttatc tttgtaagat actatcacac gctttaattc gggcatttca cttgtttctg
    12841 ccttaagata cagtggttca acaaaaagta ttgaatccct gataggcaat acaagcatgt
    12901 tgccacgaat tactcgcgaa ccacgttgat tccacagtga cagttgtttt gatatttcag
    12961 catcctgatt aatacgggct tctatctgca ttggaccaaa attcagttta tctttaggga
    13021 gggtatataa acgcaactca ccataattgg gtatatcaca tcgagctacc ataaaagcaa
    13081 tcatattgtc tttctttgca ggtgtaaaag gtaaaattaa taaaaactct gttgcaggtt
    13141 cgtttggtag cgatgttacc agatagtaac tatgcatctt ttctcttgca ctttcataaa
    13201 tttgctgtgg tatgtcccat aaatcttcat tgttataaaa aacattaata ttggtcatat
    13261 gatatcgcag taattgttgt gcctgtatat taaatagtgc ttcaggatac cgaagatgat
    13321 ctttaaaagc atcaggcatc tgctccatgg gaatgaatag atttttaaat atttttgcat
    13381 atgcttttat taccggatca ttattatcag ctatataata ccgtatagtc ccattatatg
    13441 catcaataat tatctttatg ggatttctta tataattaat tgatacacca tttgttaatt
    13501 gtattggagt tgaatatgga aaataacttg aataagtata cccatctatt atccagtaaa
    13561 tcttcccatc aactacaaca acatatggat cagaatcgta ataaataaac ggtgttatag
    13621 ccgttaccat atctacaata tttcttctga aatgaatcct gctctggttg gtgattacat
    13681 ttgaaataag tatattgaaa tcaccaaaag ctatcgcata cattgctctt ttcactagcg
    13741 aattaagaac atctccaccc gttccattgt agatggtata tttattttca tcaccatatg
    13801 gatagtcaaa ttcaccaggt gttatctttg tgttagtaat aatatatgaa ttttgatgct
    13861 caccataata tatctgtggt actgttaccg gtattgggat tgtacttttt gttggaatat
    13921 cataaagaac taattcaggt aatccttcag gggttatttt atcaactctg ctggcaatta
    13981 caccataacc atgggtaaat ataagatgat tattgagcca tgaatttttt gaaactgtta
    14041 gctttgtatt atcaagttca cgggcggaaa tatttaacgc taccttttga ttattgatag
    14101 tatagcgtac aatatctaca tctatgaaat cataatacgg ctttaatccc tgcagttgct
    14161 tatatgtttc ctttaatgga cgccaatccc ataaacgtat attgttaact acatccctgt
    14221 ttttttctaa atctctgaag gttaatgttt gtgcattttc aaacggcaac tctttaacac
    14281 cattcaatcc aaatgctatt cttgtataat taatattata ttgtatatat ggtttttctt
    14341 tttctaattc gttgggatca acaataaatc gttgctgcaa agctggaaat attgttccaa
    14401 atacaaaata tacgggtaat agtgttatta acaatataat gggcattttg aaacttctaa
    14461 tgaccatatt agcaaagaga agtatagctg caataataga tataaccatg catatgtcat
    14521 acgccaatag tttagcatga acatcggcgt atcctgcccc aaaaaatttt gcacgattgt
    14581 taaataaaag atcataagcc agtaatcggt agccagatgc aatgattaaa actatcaaac
    14641 caaataaaat tgacagatga gcccgtgcaa accttgaaaa ttccagctta ccattttgag
    14701 aaaaaatgcc attattgagt atatggaaaa aaaatgaaaa aatagtaatg aatgacaatg
    14761 aaaccagcac ccagcgaaat ataaatttat aaaaaggaag ggaaaataca aaaaaaccaa
    14821 tatccatgtt aaataagggc tcttttggaa aatcggtgaa agcaacctca tttttaaaca
    14881 ttaaatattc tttccagtaa gttgaagccc acaatcccat aaaaaaaccc agaacaacaa
    14941 ccccaataat aagtataata aaaagtaaat tacgtgttgt tccaaaaccc ggtatcctga
    15001 ttttatcaag gatgtttttt gtaaagatcc tccctttacc gcccagtatt cgtatcaaaa
    15061 taaaattaag aataaacagg gcaataaata aaccccagaa aattgtttgc acattgaatt
    15121 ttgagaaaaa taaaatccag aatatatcta aatgattata aattgaaaac catcggtaat
    15181 ctatataaaa tttaccaatt gaataaacta gaatatatcc tgtgataaaa atggttaatg
    15241 caatcaaaag tttacgattc atcatatcac cttgctaatt tttacttcat cttttcagct
    15301 ttttctttca aaattgcatt gaggcgcgct tcatcttcag gaaatgtttt aatcataacc
    15361 aaaagtaata atccacacaa aagccagaaa agatttgcta tattaaatgc cattaaacgg
    15421 ccaaacagta caatgagtgc acttataata acaggtccaa aacccttacc cagatcatcc
    15481 gtgaggttaa ataatgaaaa gatagaacct cgtgtttcag gagtatttac attcagcaat
    15541 attgcgcgta cattaggccc ggtgatagtt accgtaaatc cagttataaa gccataaatc
    15601 aatggtaacg tgggatctgg ttcaggtatg ataacctgag gcgtgtaatt tagtaacaat
    15661 gccattggaa taataccaag aagcgttgag gtgccgcaca gcattggaag atatttgggt
    15721 ttgatattat acaaccagtt accaataaaa cctccaataa atgcccccaa tattgccgca
    15781 gcacctactg tcaatacaat aagtgttgct acttctattg agtaaccctt gtcctgtgaa
    15841 taaaaatcat tcagaaaaat aaagaaaaca ccccatggta cggttccagg tatcccctgt
    15901 agaaatacca gaatatttgt ttttatttta aagagcgatt tatattcacg ccagttgatt
    15961 ttcccagtgt aaacctttcc ttcttcaata agttctttta aactttcttc ggtttttcca
    16021 cgttgaggtt ctttaacaaa caacaaaaat atgaaagcca atataaaatt aggaatggca
    16081 acaatgataa aaggtaagcg ccagccatat gctgaaccaa taaaaccagc cataagttgc
    16141 cctacagcaa taccaagccc ctgagccaat ccaatccatg cagaagcagc agccctgttt
    16201 tcatgtgaaa aataatcacc aataattgaa aatgttaagg gaagtgcacc accaatgcca
    16261 ataccggtca atgcccttaa ccagaacaac tgatcatagt tttgagcaaa accggttaat
    16321 aagcatggta attcaccaac aatgataaca aataaaaata aatttcgccg tgatatctta
    16381 tctgtcaaat agcctattgc taatgttacc aatcctccca gaatccagaa tacaaatgag
    16441 atgtgcccac ccagcatgac atcgcgttgt acatcattaa agccaaaatc atgcgcaatc
    16501 tgcgttaaat tgggagccat taaattttga tcggcaaaca aaaaaagatt catcaatgca
    16561 attaagataa cagcaaacaa ctctttttta gcaaacccgt ttttttgttt catatatcaa
    16621 acatcctcca aaaatataaa cttaacacca atgtctttgt gggcaattaa caaaatgcat
    16681 tgtaactatc atattataat aacgaaatca agaatttttt caaatttatt caacattctt
    16741 tttacatggt ctgttgtggt tttggcacta ttactattcc ttcttctcta ctgccaaaat
    16801 aattgcaagc attgtgcaaa caataataaa cagtatatac tcaaacaagc gcatctattt
    16861 caatcattga attgttatag ggagctatcg cacaaatgat gtcattaact cttttagtat
    16921 agatatattg aactaccttt aaactattat tactaaccgc tacaacggtt ggatactttc
    16981 cactccatga tggatcaatg ccactataat acattgttgt atatcactat ttaaaaaaat
    17041 aaaggggcat tgtaagttta tgcccctttt tgattaaaca aattgtatca aataaatcaa
    17101 actaacttat tttttctcca ttttttctaa ttccatctta cgaagttctt tacgcaatat
    17161 cttcccaaca ttgctttttg gcagttcatt gataaattca accatctttg gtactttata
    17221 agctgccaat tttgttttgc aataatctat gacctcttgt tcggtcattg tttcaccagg
    17281 tttcagtaca acaaatgcct taactgtttc accacgatat ttgtccggca ctccaattgc
    17341 acacgcttcc agaatctttg gatgttcaaa aagaacttca tcaatgtcac gtggataaat
    17401 attatatcca cctgctatga tcatatcctt ctttctgtca acaatataga aatatccatt
    17461 ttcatcaact ttagcaatat caccggtata gagccatcca tcccgtaatg tctttgctgt
    17521 ttcatcaggc atcttgtaat atccgctcat tacctgtggt cctctaatgc ataattctcc
    17581 ttcctgtcca atttccatct cctttgtccc ctcatccaga tcaactatct tacattcagt
    17641 gtcagggatt ggcaaaccaa tggaacctgg aattgtcttc ccaccataag gattgatagt
    17701 tacaacagga gttgtttcag ataagccgta cccttcgcat ataggtgcac ctgtttttgc
    17761 ttcaaactcc ctaataacat caaccggtaa cggagcggca cctgaaaaac aacctttgat
    17821 agttgaaata tcggcttctt taatctttgg atggtttaaa agtccaacat agaacgttgg
    17881 aacagcaggc ataaaatcaa ctttatgttt tattaatgct tctaatactg ctcctgcctc
    17941 aggacgtggt attagaacct gagtccatcc ataaaatgca ctaatattca taaccgttgt
    18001 catgccaaat gagtggaaat atggcaacac cccaatactg gtcaattcac cacgtttaaa
    18061 ttcaggaaac catgccttta tctgctgtac attagacgaa aggtttttat gtgaaagcat
    18121 tgcaccctta ctaacagcag ttgtaccacc cgtatactgt agggttgcca gatcattaaa
    18181 tttagccata gttcccggat ctttatcagg atactttttt aaaagatctg tccattcata
    18241 tacttctggc gttggaggaa tatttcgatg attggcttta gcaacaattg gtaaaagctg
    18301 ctttttagga aatcctaaat gatcacggat atgagctaca ataatctttt tcacctgtgt
    18361 tttaggtcgc aatgcaatca tacggggtcc taatagatca agggtaatga gcactgttga
    18421 ttctgagtta ttcaactggt gttccaattc atgatccgta tagagcggat tattcataac
    18481 cactactgct cctatcttcc atgctgcata tgaaccaatg actatctgtg gcatgtttgg
    18541 catgagcatt gccactttat cacccggttt tacacctaga tcaatcaatg cattggcaat
    18601 tttattaacc atggactgca tctttttaaa actaattttc gcatccagaa agatcattgc
    18661 atttttattg ggaaaatcct ttgcagatct ttcaaaacca tgcacaacgg ttatctcatc
    18721 atatttgagg gtatgcggga cacccggtgc ataagatttt aaccaaatct tttccattgc
    18781 acaaacctcc tgtgtcattt ttgtaaatta taattattaa gaatacattt actggtaaaa
    18841 atgatgaata attaaatacg acatat
//
LOCUS       DAOOJH010000040        18842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2893_length_18842_cov_8.598605, whole genome shotgun sequence.
ACCESSION   DAOOJH010000040 DAOOJH010000000
VERSION     DAOOJH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2893_length_18842_cov_8.598605"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PLJ75_06910"
     CDS             complement(<1..797)
                     /locus_tag="PLJ75_06910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPD04983.1"
                     /translation="MNLSTTLGNCTLKNPITIASGTGGYGEELSQYFDIGLLGALFTK
                     GLSLKPRSGNPGPRIVETPSGMLNSIGLENVGLEIFLQQKLPFLLQSKATVIPNVAGH
                     SVEENVELCKILSHTEGIAAIELNVSCPNVKEGGIAFGQDIKIFSQLLEKVRNVTDGA
                     LIVKLSPNVTDITQFAKAAKNIGADAVSAVNTFVGMKIDIKTGKPYFANTFAGLSGPA
                     IKPIALRMVYQITENVDIPVIGIGGIFCLEDVLEFLMAGACAVQIGTA"
     gene            1025..1693
                     /locus_tag="PLJ75_06915"
     CDS             1025..1693
                     /locus_tag="PLJ75_06915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPD04984.1"
                     /translation="MIYIAFDIDGTLYDCSTIVGDAFAQGIDEFLKNHPELDINKPST
                     QEIVNLVGTPVNEIFYTLFPRLPYNLVQQLNDICTNKLSSMVLQKKGIILPGVIETIP
                     MLYNNGYTLLTASNGRKKYVQAILDAYELSHYFFNIVALEDKRLHNKTQLLTYYKNSL
                     YDCKQLIMVGDRTNDMVAAQNNGLPFIACAFGHNAHEVEHCRWIAHSFYEIPQIVERI
                     IENC"
     gene            1771..2223
                     /locus_tag="PLJ75_06920"
     CDS             1771..2223
                     /locus_tag="PLJ75_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04985.1"
                     /translation="MYIETTTCISLEHCALLKRYSQLHGISIRTLLSSLIHYAILTEK
                     RKAQPFKRVEYRKRNQDWKRFHLYLFEDEYEFFIDVKKVWKMSLANVIAYCIDTVLPE
                     LLKLLANEDDTDNYRYRNYSFGFFKEEGIQCCKFYWGLHPEIIRKASS"
     gene            2301..4826
                     /locus_tag="PLJ75_06925"
     CDS             2301..4826
                     /locus_tag="PLJ75_06925"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD04986.1"
                     /translation="MHDIISSLNIFMLPPLITLITSLVIAGIAILKGKLKKENIFFAL
                     LCIWYALLTPNFLCHFIIKDVNTILKIERTIHTLYVFIPFIGVMFYHTVLGITRKYLE
                     ISCFVVSALFALLVHTPYYITDLLTYPWGHIAKGGIVFQIFGLYAFSILVYAIVISLL
                     KLRQEKNELVRLKIKYILLALNLIGIFTLCNVPAMMGYNFYPLSNLMFIPLVILAYGI
                     LRYRLMDIQSVLHITFIWLCISSVIVIPNIVIYTILYPYFTSIDKTVLFVLFTVWFSL
                     NYFYFRKVQPFIDQLFNRRKYDLKKVQSQFINDMGLLKDLHLLLNELSYILKRTLLVK
                     NVEIFIKDDESNNEFYNLSGEKIVIDKKLEEFLLGHPGVIEKKIVISKDEYNDIRQIL
                     IPLFEKFSSEYIISLAHNELVGFIMLSERVNLKPLSGDEIELLQNIKTASSIAIANSL
                     MYQNLNNLKNNLEMLVAQRTEELQKKNDQMEFELKLAKTVQKTLLPAVLPANNQLYAA
                     VYFKPLMEVSGDFFILEQLDETHYLIGVVDVSGHGIPSALLTSMIKTEIENLARTYLS
                     TSQICTQLNRRLTGILQESGFYFTLFLGIVDFVNMELTYTNCGHTDIYVITYNDTVYP
                     LSTDSVFIGADEHMNYPECIFELNTKDRIVLYTDGIIEIRSQNSQFYGSERFTNILVA
                     SREFSPQDQIDIIIKDLQNYIQSEQGAIRDDMTLCIIEIGEPIAVDNYLKKAVTFYKK
                     KNYDNALAILNSISTMSIPASYNYLKAKLLFKNRHYEDAKQAILLALKEKPDHKEFNY
                     LYGQICFALKDFNTALDIFADISMMQPYKKSDEFIELIRKKIE"
     gene            complement(4866..6113)
                     /locus_tag="PLJ75_06930"
     CDS             complement(4866..6113)
                     /locus_tag="PLJ75_06930"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPD04987.1"
                     /translation="MEREKVLELKQVIGKKLNRPMLYYLDLCTRCGLCYDTCHVYQGI
                     PKKEYSPVGRAEVVRRVFRKYFKPAGKFFPWWGDAHEFDENLMEELREAAFSCTGCRR
                     CTMFCPFGIDMPYILGIAKELLIAYGNAPEELVMLADGAVEKGKSIDMFKEGFRGVIK
                     DLEQEVQKLIGASSSDGLIPMEKHGANILFVGLSGAHSIIYPAAIFNAAKEDWTLSYF
                     EAVNFGYFAGDTEKSNFIANRIINEAKALGVKEIVIVECGTAYRIPKFLMGPLPFKVS
                     SIVEVIYRYIKEGRIKIKQGIVSEPVTWHDPCQLGRNGGIYEQPREILQIMANDFREM
                     NPNCEYNWCCGGGGGLVALDNEEFRIKSGKPKKEQIEKSGASMVVTACENCHLQLSTI
                     SQGYNMGVKVESLTKLVGENLII"
     gene            complement(6116..6838)
                     /locus_tag="PLJ75_06935"
     CDS             complement(6116..6838)
                     /locus_tag="PLJ75_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HPD04988.1"
                     /translation="MSGYLSYFILIPMVYIAFATLIFGLIYRFYSIWKAPAPVGTGAI
                     FPKKGSKVSGLIYDAMLVPMAYAKQKGFWFIIMLFHIAFFFLFLGHLELVADFKFIQI
                     IPHRIFLGGGLVGIILMLTTLYFLFRRFGTPYREISIPEDFIILLVLFFCILFGSILH
                     LAERYSDWGAVLRVDINVYRNWLQSLLLFKPQLSYNITELSHYTILVLHVLFANIFLM
                     MFPFSKMIHSVFTFLAHYRKRN"
     gene            complement(6863..7102)
                     /locus_tag="PLJ75_06940"
     CDS             complement(6863..7102)
                     /locus_tag="PLJ75_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HPD04989.1"
                     /translation="MSDIKADVTMDLKGLACPLPVVKVSQQIKKMQVGQVLMAETTDP
                     GALADFPAWAKTSGNEIIDTVKDGTTTKFFIKKLK"
     gene            complement(7255..7794)
                     /locus_tag="PLJ75_06945"
     CDS             complement(7255..7794)
                     /locus_tag="PLJ75_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HPD04990.1"
                     /translation="MDVNKTLESIQNQIKEIQDGRKDKIAMIVFSGDLDKLLASFIIA
                     TGSVAMGMEAVMFFTFWGTPALRDKNKKGKGKNLFGKMFGFMLPKGALKVKLSRMNMG
                     GMGTGMMKYLMKKKNIENLDGMLALAGELGVKIYVCEMSMDLMGFKMDEMIDYPGLQS
                     VGVATFLQEALDSKIQLFI"
     gene            8116..8304
                     /locus_tag="PLJ75_06950"
     CDS             8116..8304
                     /locus_tag="PLJ75_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD04991.1"
                     /translation="MNNKPVTNNEAHNDDNRLHFASTNEALYALNMYMNRFMWISTRE
                     IVSGISSWYYNDTKGEKL"
     gene            complement(8366..8581)
                     /locus_tag="PLJ75_06955"
     CDS             complement(8366..8581)
                     /locus_tag="PLJ75_06955"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPD04992.1"
                     /translation="MKKNVGTADRYIRVLIGVSLLINIFIVKPGAIGTIILLALGLAV
                     LYSAYSGYCWIYDLLKISTCKKSNDAE"
     gene            complement(8820..9755)
                     /locus_tag="PLJ75_06960"
     CDS             complement(8820..9755)
                     /locus_tag="PLJ75_06960"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD04993.1"
                     /translation="MKCTIVTVYITTMKRYCIVIIIAIALVFNTIACYKQHIPSDGVY
                     TVWVHSDIQPTKKSERWHYARAIDDIMNIKPHPIFAIIAGDIVQHKEAEEDYQWFIEL
                     RKKAEINYWYEIAGNHEWKNMPAYNTYIGKPLRYTVLFGNIIFIFMSNERGRPPTYIS
                     DETFLWWKNIVITNQDKNIITVTHAPLKNSNLIGTVSKKHIIINSERFEKVLQQYHVD
                     IWISAHIHLPAWIGQNSKIIPEFNNTLFINVYGIRKDPTTNVESRIFFFTHGSKKVLI
                     RLRDHEAETYKSTYDINHKLHYPFTCNNCLPEMIQ"
     gene            9836..11104
                     /locus_tag="PLJ75_06965"
     CDS             9836..11104
                     /locus_tag="PLJ75_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD04994.1"
                     /translation="MDNNITIDTSRFQEISVSYLKKLSGDKLLTFDVLDQQGNTVFSA
                     GQALDDELLKNKSEEGIKFFIPRVTESIGVYDREIIDMKKLKAIADDTATMYTDIRKN
                     GKMTHAQFLQSHNHLAAIVDSLRPEDKTGGVLSLLKDIKDFDYTTYTHSVNVGLLAMI
                     LVYKEMSTGDKVKLAALAGYLHDIGKLRVEQSIIQKPGLLSQDEFKKVLEHTREGYRI
                     LDSVVDSTGKKVVPNSIKYVALFHHRKLQSPGYPFRETDKDKTLENQYSELPDLARMI
                     GICDMYDALTSATPFRIPMGVEKALRYILSLSNYLYSTDDIHRFIKVLGLSLNKGTPF
                     LKAGDFIMLESEKISMDTRKKVRVYEVARIEEISRINFMNPKVMIFYDTIKNKKVHNI
                     IVDLRYDSNRRVVRVFESTRIRNMLLRQLK"
     gene            11115..11483
                     /locus_tag="PLJ75_06970"
     CDS             11115..11483
                     /locus_tag="PLJ75_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD04995.1"
                     /translation="MNPILLIFDNDRFAEFIRFVLTTAGLKIELLADPLEIFEKIKEM
                     NPPLIVMDIFLKNIDGLYLLERLYSVKEFTKIPVFIVSSRNEPLAVFDALERGAADYM
                     AAPINEDMLIEKVNKLLQAN"
     gene            11605..12630
                     /locus_tag="PLJ75_06975"
     CDS             11605..12630
                     /locus_tag="PLJ75_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HPD04996.1"
                     /translation="MKKCILLVLAVLLFLLECSSRINLYNTSSDGQSNNTINAYSMAE
                     SLSFNEVWAYLMKGEEDKLTGEEKITDLCYFSAKVNGKGQITDDYAPPAIKAENRIRH
                     HIVIAILDNYALTHFIIHPDFKVRKGFIKDICRISQKFDGVQIDFESVASSDAHYFFS
                     FLSELRANLGKSKVVSIALPARRSRIADAYDYPSIAALVDRIIIMAYDQHWYGSNPGP
                     IAGLSWCREVGEFAKSFIPQQKLIMGLPLYGRCWQDKNFNKSMHYTHIEQVMQTSNIQ
                     PRYDPENGSYFEYEEKIKVRVYFNDERALYERLKLYSSMSIDNVAFWRIGQGPKGMWQ
                     GISTKSL"
     gene            12656..13189
                     /locus_tag="PLJ75_06980"
     CDS             12656..13189
                     /locus_tag="PLJ75_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPD04997.1"
                     /translation="MKKLSYDDIQSNRPDKNELFRLQRFPVVTILENIRSLYNVGSMF
                     RTADAARIAYMYLCGYTPHPPRKEIEKTALGSTESVPWEHSTKPIQIIRMLKNQGFVI
                     AALEHTTTGVAYTTYNYSYPLCIVVGNEVHGVSDDVLAMCDAAIEIPMFGIKHSLNVA
                     VAYGIVVYHAVMTYQMK"
     gene            13208..14371
                     /locus_tag="PLJ75_06985"
     CDS             13208..14371
                     /locus_tag="PLJ75_06985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPD04998.1"
                     /translation="MQKVILKQGKEKSVLQRHPWVFSGAIATVDADDGAMVPLCSYDG
                     TVIAWGYYNSRTQIAMRLLTFSDTKPDDDLIARRLMNAYALRVESGVVKSTNAFRMVH
                     SEGDMLPGLIADWYNGHCVVQFLTSGMDRLKGTIVQIIQEKLQPFSIYERSDHEGRKM
                     EGLPEVHAQLHGQTPDFITINEHGMEFYVDVIQGQKTGFYCDQRDNRLRIQSIANGKD
                     VLNLFAYSGGFSVAALMGGATKAVSVDSSGYALSIAQKNCELNNVGKRHAGVKADVFD
                     YINTNDIPQNVIICDPPALIKSKSAIQQGLRGYKELNVKIFKKVPPLSFVLTCSCSRF
                     VDMKLFQKIVFSAARDAGREVTITGKYLQPADHPVSIYCPETEYLKAIMVFIS"
     gene            14541..15380
                     /gene="nifU"
                     /locus_tag="PLJ75_06990"
     CDS             14541..15380
                     /gene="nifU"
                     /locus_tag="PLJ75_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HPD04999.1"
                     /translation="MWEYTDKVKELYTNPKNVGEIPDADVVVEVGSIVCGDALTLYLK
                     LDGDVIVDAKFKTFGCGSAIASSSALTEMIKGKTIAEAARITNKDIVDFLGGLPDQKM
                     HCSVMGQEALEKAIKKIKGETIEDEQEDEGAVICQCFGVTENVIRRVIRENNLATVED
                     VTNYTKAGGACGSCIPKIEDILKEELASAVAQPAATAMKPMSNIKRMQLVEETIHNVI
                     RPVLMRDGGDIDLVDIEGKKVYVALRRHCAHCVVSDVTMKNLVEDTLKEFVENDIEVI
                     EVK"
     gene            15380..16588
                     /gene="nifS"
                     /locus_tag="PLJ75_06995"
     CDS             15380..16588
                     /gene="nifS"
                     /locus_tag="PLJ75_06995"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPD05000.1"
                     /translation="MNTIIYLDNNATTMVAPEVLEAMLPFFTRRYGNPSSMHDFGGDV
                     EHDMEKARNQVARMLGAEYDYEIVFTSGATESDNMAILGTLQYYRDKKHIITTKVEHP
                     AVLNLCRQLEREGYSVTYVPVDREGNLDVDFLFDSVTDDTAIVSVMYANNETGVIFPV
                     ETIGAFCKQRGITFHIDAVQAIGKIPVDVNALQCDLLAISGHKFHAPKGIGVLYVRRG
                     TRLRPLLYGGHQEKARRPGTHNVPGIVGIGKAAELVMQHLSNSEEVANVKRLRDRLEN
                     GLLSQLSNAHLNGNKNNRVDNTTNIGFEFIEGEAILLYLNEKGIAASSGSACSSGSLE
                     PSHVLRAMGVTFTSAHGSIRFSLSRYTTEDEIQYTLQVMPEVVNRLLAISPYWDAMNK
                     KGKSMTELAH"
     gene            complement(16615..17451)
                     /gene="flgF"
                     /locus_tag="PLJ75_07000"
     CDS             complement(16615..17451)
                     /gene="flgF"
                     /locus_tag="PLJ75_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HPD05001.1"
                     /translation="MVRGLYTGASGMIAQEARLDAIANNLANVDKAAYKKDLTLFKAF
                     PDMIIRRLNDDGLGITPAGSYDTMPFVGKLGTGVEVNEVYTIHEQGSLMRTENPFDMA
                     MEGNGFFTVMTERGERYTRNGAFTLNKDGILVTHSGLPVLGQNGIIKLQKNNFMINER
                     GEILVNSALSLNPDDMIGLTQNNWEQPVVIDKLKIVDFENIRELKKEGESLYHETQYS
                     GPALPAEEYKVIQGFLEKSNVNAVREMVDMIEVQRSYEANQKAMLTHDQELGRLINEV
                     AR"
     gene            complement(17451..18719)
                     /gene="murA"
                     /locus_tag="PLJ75_07005"
     CDS             complement(17451..18719)
                     /gene="murA"
                     /locus_tag="PLJ75_07005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPD05002.1"
                     /translation="MDFYIVKGGNKLNGTVTISGAKNAALPIIVASLLAEGTTVLHNV
                     PNLKDIQTILQVIECLGAQYQFDINTNTLTIHVNSLKNAEVPYDLVKTMRASIYVMGP
                     LLARCGEADVSLPGGCAIGERPVDIHLSGFEALGTTITIEHGYIKARVEKLNGNRFIM
                     RKVSVGATANIMMAAVLADGQTILENCAMEPDVVDLGNFLITMGAKIEGLGTERIIIN
                     GVKKLKPVSYSIIPDRIEAGTFLIAGAITRGNVTVNNVIPEHVKACIDTCADMGFVIS
                     GNDSSVTIEPSKKLQGSMVRTLPYPGFPTDLQAPIMSLMCTIPGISVIIETIFENRYT
                     HVGELRRMGADIEIEGNTAIIKGGKQLQAAPVLMSDLRAGASLVLAALAARGKTEIRR
                     IYHTDRGYENFEQKLQALGADIVRVKGGKP"
BASE COUNT         5938 a         3359 c         3622 g         5923 t
ORIGIN      
        1 gcggtgccaa tttggacggc acatgcacca gccattaaaa attcaaggac atcttcaagg
       61 caaaatatgc ccccaatgcc aatcaccgga atgtccacat tttctgttat ttgatacacc
      121 atcctgaggg ctataggttt tattgcagga ccacttaaac ctgcaaatgt atttgcaaaa
      181 tacggttttc cggtctttat atcaatcttc attcctacga aagtatttac tgctgatact
      241 gcgtctgctc caatgttttt ggctgctttt gcaaattgtg taatatcggt aacattgggc
      301 gatagtttta caataagtgc accatcggtt acattacgaa ccttttcaag gagttgtgaa
      361 aatatcttga tatcctgacc aaaagcaata ccgccttctt ttacattggg gcatgacaca
      421 ttcaattcta tagctgcaat cccttcagta tgcgatagta ttttgcacaa ctcaacattt
      481 tcttctactg aatgaccggc aacattgggt attaccgtgg cttttgattg taataaaaat
      541 ggtaattttt gctgtaaaaa tatttccaga ccaacatttt ccaatccaat tgagttgagc
      601 atgccagatg gcgtttccac aatccttggt ccaggatttc cggatcgtgg tttaagcgat
      661 aatcccttgg taaacagtgc acccagtaaa cctatatcaa agtattgaga aagttcttcg
      721 ccgtaaccac cagtgcctga agcaatggta attggatttt tcagtgtgca atttcctaat
      781 gttgttgaaa gattcacaag catgccatgt gttatttatt agaaatataa tcgtgaaata
      841 gtattatatc acagatgcgt caagaaaatt acaatgcaac aataaaattt tcaaaaaatt
      901 aattgtatgt attcatgttg actatatcac cgtttgatat gaacagtgtt aaaactgtta
      961 aaaaaggtag catgtgtatt atagtattgt aatagcacaa attttatttg ttagatggtt
     1021 ggtcatgata tacattgcgt ttgatattga tgggacacta tacgactgct caacgattgt
     1081 tggcgatgca tttgcgcagg ggatagatga gtttttaaaa aatcatcccg aacttgatat
     1141 taacaaaccg tcaactcagg aaatagtgaa cctggtaggt actcctgtta acgaaatatt
     1201 ttatactctg tttccccgct taccttacaa tttggtgcaa caactgaatg atatctgtac
     1261 caataaatta tcatctatgg tattacaaaa gaaaggtatt attttaccag gagttatcga
     1321 aacaattcca atgttgtata ataatggtta tacactgtta accgcatcga atggcagaaa
     1381 aaaatatgta caggcaatac ttgatgctta tgaactatcg cactattttt tcaatatcgt
     1441 tgcgctggaa gataagagat tgcacaataa aacacaattg ctgacatatt ataaaaatag
     1501 tctctatgat tgtaaacagc tcattatggt tggcgataga accaatgata tggtggctgc
     1561 acaaaataat ggattgccat ttattgcctg tgcttttggg cataatgctc atgaggttga
     1621 gcattgccga tggattgctc attcatttta tgagataccg cagattgttg aaagaataat
     1681 cgaaaattgt tgatttatgg tgtgcttgtt gcatactgct taaccatgta cgacagtcat
     1741 ttttaatgtt ttttaagata tggagggttt atgtatatag aaacgacaac ctgtatttct
     1801 ttagagcatt gtgctctatt aaagcgttat tcacaattgc atggcatatc aatacgaacg
     1861 ttactatctt cattgattca ttatgcaata ctaacagaaa aaaggaaagc tcagccgttc
     1921 aaaagagttg aatacaggaa aagaaatcaa gattggaaac gttttcatct gtatttattt
     1981 gaagatgaat atgaattttt catagatgta aaaaaagttt ggaagatgtc gcttgcaaat
     2041 gtcattgctt attgtatcga tactgttctt ccagaattac taaaattgct tgcaaatgaa
     2101 gatgataccg ataactatcg gtacagaaat tatagttttg gattttttaa ggaagaaggt
     2161 atacaatgct gcaagtttta ttggggatta caccctgaaa taatacgaaa agcatcctca
     2221 tgacacaaaa tacaattgac tatatcgtta tatcacattt aaagtggtat tattcttttg
     2281 agtactgatg aggttactgt atgcacgata tcatttcgtc gcttaacatc tttatgctcc
     2341 cgcctcttat aacccttatt accagtttgg tcattgctgg tatagctata ctgaaaggaa
     2401 aattaaaaaa ggaaaatata ttttttgctt tattatgtat atggtatgca ctgcttactc
     2461 ctaatttttt atgccatttt atcatcaagg atgttaatac cattttaaaa atagaaagaa
     2521 ccatacatac gctgtatgta tttattccct tcataggggt aatgttttac catacagtat
     2581 tgggaataac aagaaaatat ctggaaataa gttgttttgt tgtaagcgcg ctgtttgctt
     2641 tgttggtgca cactccttat tatataacgg atcttttaac ctatccatgg ggacatattg
     2701 caaaaggggg tatagtattc caaatttttg gattatacgc tttctcaatc cttgtatatg
     2761 ctatagtcat aagtttatta aaattaagac aggaaaaaaa tgaactggta cgcttaaaaa
     2821 tcaaatatat acttcttgca ttaaatttaa taggtatttt taccctctgt aatgtcccgg
     2881 caatgatggg gtataatttt tacccattga gcaatttaat gtttatccct cttgtaattt
     2941 tagcatatgg cattttgcgg tatcgtttaa tggatattca aagtgtattg cacataacat
     3001 ttatatggct ttgtatttct tcggtgatag ttattcccaa tatagttatc tataccatat
     3061 tatatcctta ttttacatca attgataaaa cggtactttt tgtattattt acagtatggt
     3121 tttcacttaa ctatttttac ttcagaaagg tacaaccatt tattgaccag ctttttaata
     3181 gaaggaagta cgatttaaaa aaggtacaat cgcagtttat caatgacatg gggttgttaa
     3241 aagatctcca tcttttactc aacgagctgt catacatttt aaaacggaca cttctggtta
     3301 aaaatgttga aatatttatt aaagatgacg aaagcaacaa tgagttttac aatctttcag
     3361 gtgaaaagat tgtaattgat aaaaagcttg aggaattttt attgggtcat ccgggtgtaa
     3421 tagaaaaaaa gatagttata agcaaagatg aatataatga cataaggcaa attttgatac
     3481 cattatttga aaaattttct tctgagtata tcatatcgtt agcgcataat gaacttgttg
     3541 gttttattat gttatccgaa cgtgtaaatc taaaaccact ttctggtgat gaaattgaac
     3601 tattgcaaaa tatcaaaaca gcttcctcaa tagcaatagc caattcatta atgtatcaaa
     3661 atctaaataa tttaaaaaac aaccttgaaa tgctggttgc acagcgaacc gaagaacttc
     3721 aaaagaaaaa tgaccagatg gaatttgaac ttaagttagc aaaaacagta caaaaaacgt
     3781 tattgccggc agttcttcct gctaataacc aattgtatgc ggctgtttat tttaaacctt
     3841 taatggaagt aagcggcgat ttctttattc ttgaacaact tgatgaaaca cattatctta
     3901 ttggtgttgt tgatgtttcg ggccatggca taccatctgc attgcttacc tcaatgataa
     3961 aaaccgaaat agaaaattta gccaggacat atttgtcaac atctcaaatt tgtacacaat
     4021 taaatagaag gttgacgggc attttgcagg aaagcggctt ttattttacc ttatttttag
     4081 gcatagttga ttttgtaaat atggagctta cttataccaa ttgtgggcat accgatattt
     4141 atgttatcac ttataatgac acggtatatc ccttgtctac tgattctgta tttataggtg
     4201 cagatgagca tatgaattat cctgaatgta tatttgagct caatacaaaa gaccgtatcg
     4261 tactctatac cgatggaatt attgagataa gatcacaaaa tagccagttt tatggcagtg
     4321 aacgttttac caatatactt gtggcttcaa gggaattttc gcctcaggat cagattgata
     4381 ttataatcaa ggatttgcaa aactatatac aatccgagca gggggctatc cgtgatgata
     4441 tgacactgtg tatcattgaa attggtgagc ccatagctgt tgacaattat ttaaaaaaag
     4501 cagttacatt ctataaaaag aaaaattatg acaatgctct tgccatttta aattcaattt
     4561 ctacaatgtc catacctgca tcatataact atctcaaagc aaagttatta tttaaaaata
     4621 ggcattatga agatgctaaa caggctattc tgttagcgtt aaaagaaaaa ccagatcata
     4681 aagaatttaa ttatctttat gggcagatat gttttgcttt aaaagatttt aatacagcgc
     4741 tggatatatt tgctgacata tcaatgatgc agccatataa aaaaagtgat gaatttatag
     4801 aattaattag aaaaaaaata gaatgatata taaggggcaa atatctttgc cccttcatgg
     4861 ttacgttata taattaagtt ctcgcctaca agttttgtta aggattcaac ctttactccc
     4921 atattatatc cctgacttat agtagacagt tgtaaatgac aattttcaca ggcagttaca
     4981 accattgaag caccgctctt ctcaatttgc tcctttttag gtttgccact tttaatcctg
     5041 aattcttcat tatcaagcgc aactaaacca ccgccgccac cgcagcacca gttatattcg
     5101 cagtttggat tcatttcacg aaaatcgttt gccattattt gcaatatctc acggggttgt
     5161 tcgtaaatgc ctccattcct tcctaactga cagggatcat gccatgttac cggttctgat
     5221 acaatgccct gtttaatttt gattctgcct tctttgatat agcggtaaat tacctcaacg
     5281 atgctgctta ctttaaaagg caatggcccc attaaaaatt ttggtatcct gtatgctgtg
     5341 ccgcattcaa caataactat ctctttgact cccagtgctt ttgcctcatt gatgattcta
     5401 ttggcaataa agtttgactt ttctgtatca cctgcaaaat agccaaagtt taccgcttca
     5461 aaatagctca gcgtccagtc ctctttagcg gcattaaaga ttgcagcagg ataaattatt
     5521 gaatgcgccc ctgataagcc aacaaaaagt atattggctc cgtgtttttc catgggaatt
     5581 aatccatcac ttgatgatgc accaataagc ttttgaactt cctgttctaa atcctttatt
     5641 accccacgga agccttcttt aaacatatcg atgcttttgc ctttttcaac cgcaccatca
     5701 gctagcataa ccagttcttc aggtgcattg ccatacgcta tgagcaattc ttttgcaatc
     5761 cccaatatat agggcatatc tataccaaaa ggacaaaaca tggtgcaacg gcgacagccg
     5821 gtacatgaaa atgctgcttc acgcagttct tccataaggt tttcatcaaa ttcatgtgca
     5881 tcaccccacc atgggaaaaa ttttcctgct ggcttaaaat attttctgaa aaccctgcgg
     5941 acaacctcag ccctgcctac cggtgaatac tctttttttg gtataccctg gtaaacatgg
     6001 caggtatcat aacacaaacc gcatcgtgta cagagatcaa gataatacag catgggtctg
     6061 ttcaactttt ttccaatgac ctgtttaagt tccagtactt tttccctttc catggttagt
     6121 tccttttacg gtaatgtgct aaaaaagtaa aaaccgaatg tatcattttg ctaaatggaa
     6181 acatcatgag gaagatgttg gcaaaaagaa catgcagtac cagtattgta tagtgcgata
     6241 gttcagtaat attatacgat agctggggtt taaacaatag caaactttgg agccagtttc
     6301 tgtatacatt aatatcaacc ctgagcactg caccccaatc gctgtaccgt tctgctaaat
     6361 ggagtattga accaaaaagt atacaaaaaa acaaaacaag caggatgata aaatcttccg
     6421 gtatggagat ttcacggtag ggagttccaa atcgtctgaa gaggaaatac agtgttgtaa
     6481 gcataagaat gatgcccact aaaccgccac ccagaaaaat tctatgaggg ataatctgga
     6541 tgaatttaaa atcagctact aattccagat gaccaagaaa aagaaaaaag aatgcaatgt
     6601 gaaaaagcat tatgataaac cagaaacctt tttgcttagc gtatgccatc gggaccagca
     6661 tagcgtcata gatgagtccg gatacctttg aacctttttt aggaaatata gctcctgtac
     6721 ccacaggagc aggagctttc catatactat aaaatctata gataagccca aaaatcaagg
     6781 ttgcaaaggc tatgtaaacc ataggaataa gaataaaata tgataaatag cctgacattg
     6841 ttcacctcac agcattactg tgttatttta gctttttgat aaaaaatttt gtagtagtac
     6901 catctttcac agtatcaatg atctcattac cactggtttt tgcccatgcc ggaaaatctg
     6961 caagagcacc gggatcggtt gtttctgcca ttaagacttg tccaacctgc atctttttaa
     7021 tctgctgtga tacttttaca acaggtaatg gacaggccaa tccttttaaa tccattgtta
     7081 catcagcttt aatgtcactc ataatttcct ccttaattaa ttgttacaat actatatact
     7141 atatactata tactatatac tataacatta acaatacaat aacttcccgt cattcttact
     7201 tatgtataaa gtaataacat atataagaca agaataaatt taaaaaaatt tttattaaat
     7261 aaaaagctga atcttgctat cgagtgcttc ctgtaaaaag gttgctacac caactgattg
     7321 cagtcctgga taatcaatca tttcatccat tttgaaaccc atcaaatcca ttgacatttc
     7381 acatacataa atttttaccc caagctctcc ggctaaagct aacatgccat ctaaattctc
     7441 aatattcttc ttcttcatca agtatttcat cataccagtc cccattccac ccatgttcat
     7501 tctggagagt ttaaccttta atgcaccttt tggtaacata aaaccaaaca tcttcccaaa
     7561 aagatttttg cctttcccct ttttgttctt gtctcgtagg gcaggagtgc cccagaatgt
     7621 gaaaaacata acagcttcca tgcccattgc tacagaacct gttgcaataa tgaaggaagc
     7681 taatagctta tcaagatcac cactgaatac aatcatagca atcttatctt tccttccatc
     7741 ctgaatctcc ttaatttgat tctggatgga ttctaatgtt ttgttaacat ccatacattc
     7801 cccctggatt atagtttttt gataatttaa aattttataa taaactttaa tactaattaa
     7861 actatactac aattttgtaa ataacaaaaa tttttttatt gaaaattaat attttatgat
     7921 tctacaatag ctatggattg tactatacag tatacgtata gtatgtgcga tagttccata
     7981 caataaaagc ttaacattaa tattttttta ctctatgaat gtgcattgtt gacagttgtt
     8041 gtaagaagat gtacggtggt gattttatgt agcgatgctg ctgcacaata gtatatattg
     8101 aataaagagc acacagtgaa taacaaacct gttactaata atgaagctca taatgacgac
     8161 aatcgacttc actttgccag tacaaacgaa gcactctatg ctttgaatat gtatatgaat
     8221 agatttatgt ggatttcaac aagagagata gtttccggaa taagctcatg gtactataat
     8281 gacacaaaag gggagaaact ataaaataat aaggggtaca ccatgtcaga ccccttatta
     8341 ttttatttta atgaaactat tgttgttatt cagcatcatt tgatttttta catgtgctta
     8401 tctttagaag atcatatatc cagcaatagc cgctatatgc tgaatataaa acagccagtc
     8461 ccaatgccag taaaatgatt gtacctatag cgcccggttt tacaataaaa atattaatta
     8521 ataaagaaac tccaataaga actcgtatgt acctgtctgc tgtaccaaca ttttttttca
     8581 tagctcacct cggttaatca atattaaact ataatgaaat atcacaaagt aaatctttta
     8641 acattaagtg aatatggcaa gtattttttt atatatctgt tataaaagat aattgcaata
     8701 ttgcaattga tagtattatg ggtaccgcta aaactatatt ttcattatta aaaaatagct
     8761 ggaaacaata ataaagcctt ttaccaatta accaatgcct gtgcccacac aaaaatacac
     8821 tattgtatca tttcgggaag acaattatta caggtaaaag gatagtgcag tttatgattg
     8881 atatcataag tacttttata tgtttctgct tcatgatcac gcaatcgtat taaaactttt
     8941 tttgacccat gagtaaagaa aaatattcgt gactcaacat tggttgtcgg gtcttttcgt
     9001 ataccataaa cattgataaa aagagtattg ttaaattcag ggatgatttt actattctgg
     9061 cctatccatg caggtaagtg tatgtgtgca cttatccata tatcaacgtg gtactgttgt
     9121 aaaactttct caaatcgttc tgagtttatg attatatgct tttttgatac tgtccctatt
     9181 aaattgctgt tttttaatgg tgcatgcgtt actgttatga tatttttatc ctgatttgta
     9241 ataacaatat ttttccacca taagaaagtt tcatcggaaa tataggttgg tggcctgcca
     9301 cgttcatttg acatgaatat gaaaataata tttccaaaaa gtacagtata acgtaatggt
     9361 ttgccaatat atgtattata tgctggcata tttttccatt cgtgatttcc ggcaatttcg
     9421 taccaatagt taatttctgc cttctttcgt aattctataa accattgata atcttcttct
     9481 gcttctttgt gttgtacgat atctcctgct ataatggcaa agataggatg cggtttaata
     9541 ttcataatat cgtctatagc ccttgcataa tgccatcgtt ctgatttttt tgtgggttga
     9601 atatccgagt gtacccagac ggtatatacg ccatcggaag gaatatgctg tttataacag
     9661 gcaatggtgt tgaagacgag tgcaattgct attatgatga ctatacagta tcgtttcatg
     9721 gtggttatat atacagtaac tatcgtacat ttcatgaaaa ataaagaaaa aaaatattgc
     9781 aactattaca atagtgtatc ataattgatt caaatttaaa atattttagc agactatgga
     9841 taacaatatt actattgata catcccgttt tcaggagata tctgtttcgt atttaaaaaa
     9901 attaagtggt gataagcttt taacctttga tgtattggac cagcagggga atacagtatt
     9961 ttctgccggg caggcgcttg atgatgagtt gttaaaaaat aaatctgaag aaggcataaa
    10021 attttttatt ccccgtgtta ctgaatcaat tggtgtttat gaccgtgaaa tcattgacat
    10081 gaaaaagtta aaggccatag cagatgatac ggcaacgatg tatacggata tacgtaaaaa
    10141 tgggaaaatg acccatgctc agtttttgca atcacataat catttagcag ccattgtgga
    10201 cagtttgcgt cctgaggata aaaccggtgg agtgctatcg cttttaaaag atataaaaga
    10261 ctttgattat accacctata cccattcggt caatgttggt ttgcttgcaa tgattttagt
    10321 atataaggag atgtcaaccg gtgataaggt taaattggca gcgcttgcag gatatcttca
    10381 tgatatcggg aaactgcgag ttgagcaatc tatcattcag aaaccaggtc tgttatcaca
    10441 ggatgaattt aaaaaggtgc tggaacatac tcgtgaaggg tatcgaattc tggacagtgt
    10501 cgttgattca acagggaaaa aggttgttcc caatagtata aaatatgttg cactatttca
    10561 ccatcgtaaa ttgcaaagtc cgggctatcc attcagggaa accgataagg acaaaaccct
    10621 tgaaaatcaa tacagtgaat tgcccgattt agcccgtatg attggtatat gtgatatgta
    10681 tgatgcctta acaagtgcaa caccattcag gattcccatg ggtgttgaaa aagctttacg
    10741 atatatatta agtttatcca attacctgta ttcaactgat gacatccata ggtttattaa
    10801 ggtactgggg ctatccttaa ataagggtac tccattttta aaagcagggg atttcatcat
    10861 gcttgaatct gaaaagataa gcatggatac acgcaaaaag gtaagagttt atgaggtagc
    10921 acggattgag gaaatttcac ggataaactt catgaatcct aaggttatga tattttatga
    10981 tacaataaaa aacaaaaagg tacataatat tattgtggat ttacggtatg acagcaatcg
    11041 cagggtggtg cgcgtttttg agagcacacg aatacgcaat atgctattga gacagcttaa
    11101 gtagggtaga aagtatgaat ccaattttat taatatttga taatgaccgt tttgctgaat
    11161 ttatacgctt tgtattaaca actgctggct taaaaattga attattagcc gatccactgg
    11221 aaatttttga aaagattaaa gagatgaatc caccattaat agttatggat atttttttaa
    11281 aaaacatcga tggcttatat ttactggagc gattatattc tgttaaagaa tttacaaaaa
    11341 taccggtttt tattgtatca tcccgcaacg aacctttagc tgtttttgat gcattagagc
    11401 gaggagctgc tgattatatg gcagcaccaa tcaatgaaga tatgttaatt gaaaaggtca
    11461 acaagttatt acaagctaat tgaaagtcaa ttgaaacaaa aagtattgaa taaagccgta
    11521 atataccgac atatgtaatg aggtattatt gtaattttaa taacaaatgg gaagtttcta
    11581 ctattccatt ttcaaggatt tattatgaaa aaatgtatac tattggtgct tgcagtgctt
    11641 ctatttttac tggaatgttc atcaagaatt aatctctata atacatcatc agatggacaa
    11701 tcaaataaca ccatcaatgc atattcaatg gcagaaagcc tttcctttaa tgaggtatgg
    11761 gcatatctta tgaaaggtga agaggataag ctgaccggtg aggaaaaaat taccgatttg
    11821 tgttatttca gtgctaaggt aaacggcaaa ggacaaatta cagacgatta cgcaccacct
    11881 gcaataaaag ctgaaaatag aatacgccac catatcgtta tagcaattct tgataattat
    11941 gcactaacac attttataat acatcctgat tttaaggtgc gaaaaggttt tattaaggat
    12001 atttgccgga tttcacaaaa atttgatgga gttcagattg attttgaatc cgttgcttca
    12061 tcagatgctc actatttttt tagcttttta tcagaattac gcgccaatct gggtaaaagc
    12121 aaggtagtat caattgcttt gccagcacgg agaagccgca ttgcggatgc gtatgattat
    12181 ccttctattg cagcactggt ggataggatt atcatcatgg catatgatca gcattggtat
    12241 ggtagtaacc ctggtcctat tgcagggcta tcatggtgca gagaggttgg tgaatttgca
    12301 aagagcttta taccgcagca aaagctaatc atgggtttgc cgttatatgg acgatgctgg
    12361 caggataaaa attttaataa atcaatgcat tatacccata tagaacaggt tatgcaaaca
    12421 tccaatatcc agccccggta tgatcctgag aatggatcat attttgaata tgaagaaaag
    12481 attaaagtgc gcgtatattt taatgatgaa cgtgcattat acgaaaggct aaagctgtac
    12541 tcatcgatgt ctattgacaa tgtggcattc tggcgtatag ggcagggacc aaaagggatg
    12601 tggcagggta tcagcacaaa aagtttataa caatgttact ttatgaaaaa aagtaatgaa
    12661 gaaattatcc tacgacgata tacagagcaa tcgtcccgat aaaaatgaat tattcagatt
    12721 acagcggttt ccggttgtaa ccattctgga aaatattaga agcctgtata atgttggctc
    12781 catgttcagg actgctgatg ctgcgcgaat tgcctacatg tatctctgtg gctatactcc
    12841 acatcctcca cgaaaagaga ttgaaaaaac tgcgctgggc agtaccgaaa gtgtgccgtg
    12901 ggagcattct accaaaccaa tccagataat acggatgtta aaaaatcagg gttttgtgat
    12961 tgcagcactg gagcacacaa caacaggtgt agcgtataca acctataatt attcctatcc
    13021 actgtgtatt gttgttggca atgaggtgca tggtgtaagc gatgatgtac tggctatgtg
    13081 tgatgcggct attgagatac caatgtttgg catcaaacat tcgctcaatg tagcggttgc
    13141 gtatggtatt gtggtgtatc atgccgttat gacatatcag atgaagtagt agcgcagtat
    13201 aatgactatg caaaaagtaa ttttaaaaca aggaaaagaa aaatcagtac tgcaaaggca
    13261 tccatgggtt ttttcagggg cgatagcaac tgttgatgca gatgatggtg caatggttcc
    13321 tctgtgttca tatgatggta ctgtgattgc atggggatat tacaacagcc gaacacagat
    13381 agccatgcgg ctattaacat tcagcgatac aaaacctgat gatgatttga ttgcccgacg
    13441 attgatgaac gcgtatgcat taagggttga atccggggtt gtaaagagta ccaatgcctt
    13501 caggatggtg cattctgaag gagatatgct gccgggactc attgctgact ggtataatgg
    13561 gcattgtgtt gtacagtttt taacatcagg gatggatcgc ttaaaaggaa ctatcgtgca
    13621 gattatacag gagaagctgc agccgttcag tatttatgag cgcagtgatc acgaaggaag
    13681 aaagatggaa gggctgccgg aagtacatgc tcagcttcat gggcaaaccc cggatttcat
    13741 taccataaac gaacatggga tggaatttta tgttgatgtg atacaagggc aaaaaactgg
    13801 tttttactgt gaccagcgtg ataaccgatt gcgcattcaa tctattgcca atggcaaaga
    13861 tgtcctcaat ctgtttgcat attccggtgg gttttctgtt gcggcattga tgggaggtgc
    13921 aacaaaggct gtctcggtcg atagctccgg atatgctctt tcaattgcac aaaaaaattg
    13981 tgaattaaat aatgttggca aacggcatgc aggtgtaaag gcggatgttt ttgattatat
    14041 caataccaat gatatccccc agaatgtaat catatgtgat cctcctgcgc ttattaagag
    14101 taaatcggca atacagcagg gattgcgcgg ttataaagaa ttaaatgtaa aaatttttaa
    14161 aaaggtgcct ccgctgtcat ttgtattaac atgttcatgc tctcgttttg ttgatatgaa
    14221 attgtttcag aaaattgtgt tttctgctgc acgtgatgcc ggccgtgagg ttactattac
    14281 cgggaaatat ctacagcctg ccgatcatcc tgtcagtata tattgtcccg aaacagaata
    14341 cctcaaagcc attatggtat ttatttcata gtaactatcg cccgcagagt attttttaaa
    14401 atttttattg acaaacataa aatatactac tatattggta gtaattaagg attgagcaat
    14461 tggcgataga gagtaacaac gattgtgtgc tgattttgcg ttgtgatgta ttgtataatg
    14521 cgcggtaaca ggagataacc atgtgggaat ataccgacaa agtaaaagaa ttatatacca
    14581 accctaaaaa tgttggtgaa atacccgatg ccgatgttgt tgttgaggtt ggtagtattg
    14641 tgtgcggcga tgctctgaca ctgtatttga agcttgacgg cgatgtaatt gtagatgcta
    14701 aatttaaaac atttggctgc ggcagtgcaa tagcgtcgtc ctcggcacta accgagatga
    14761 taaagggtaa aaccattgca gaagcagcaa gaattaccaa taaggatata gttgactttt
    14821 taggtgggct tccggaccaa aaaatgcact gctcggtcat ggggcaggaa gcactggaaa
    14881 aagccataaa aaaaataaaa ggtgaaacca ttgaagatga acaagaagat gagggagcgg
    14941 ttatatgcca gtgttttggt gttactgaaa atgtaatacg tcgggttatc agggaaaata
    15001 atcttgccac ggttgaggat gttaccaatt acaccaaagc tggaggtgct tgcggttcat
    15061 gtataccaaa aattgaggac attctgaaag aagaattagc cagcgctgta gcgcaaccag
    15121 cagcaactgc catgaagcca atgtcaaata taaagcgtat gcagcttgtt gaagagacaa
    15181 tacacaacgt tatacgccct gttttgatga gggatggcgg tgatatcgac cttgttgata
    15241 ttgaaggaaa aaaagtatat gtagcgttac gcaggcactg tgctcattgt gttgtttcag
    15301 atgttaccat gaaaaatctg gttgaagata cattaaaaga gtttgtagaa aacgacatcg
    15361 aagtgataga ggtaaagtga tgaataccat tatatatctg gataataatg caacgaccat
    15421 ggttgcacct gaagtgctgg aagctatgtt gccgtttttt acccggcgct atggcaatcc
    15481 atcaagcatg catgattttg gtggtgatgt tgagcatgat atggaaaaag ctcgcaatca
    15541 ggtggcacgc atgctgggtg cggaatatga ttatgagata gtttttacaa gtggtgcaac
    15601 cgaaagcgat aacatggcaa ttctgggaac attgcagtac taccgtgata agaaacacat
    15661 cattacaact aaggttgagc atccggcagt attaaattta tgcagacagc ttgagcgcga
    15721 aggatatagc gttacctatg ttcctgtaga cagggaaggc aaccttgatg tggattttct
    15781 ttttgattcg gttaccgatg atactgctat tgtatcggtt atgtacgcca acaatgaaac
    15841 gggtgtaatc ttcccggtgg aaacaattgg cgctttttgc aaacagcgcg ggattacatt
    15901 ccatattgat gcagtacagg caattggcaa gattcctgtt gatgtgaatg cattgcaatg
    15961 cgatttgctg gcaatatcag ggcataagtt tcatgcaccc aaagggattg gagtgctcta
    16021 tgttcgaaga ggtacacggc tacgaccgtt actgtatggt ggacatcagg aaaaagcccg
    16081 caggcctggc acccataatg tgcccggtat tgttggtatt ggcaaagcag ccgaactggt
    16141 aatgcagcat ctatcaaatt ctgaagaggt ggctaacgtt aaacgtctgc gcgacagatt
    16201 agagaatggc ttgctgtcgc aattgagcaa tgcgcatttg aacgggaata aaaacaacag
    16261 ggtggataat accaccaata ttggctttga atttattgaa ggggaggcaa ttttattgta
    16321 tctcaacgaa aaggggattg ctgcttcttc tggctctgcc tgttcaagcg gttcacttga
    16381 gccatcacat gtgttgcgtg caatgggggt gacgtttacc tctgctcatg gttctatcag
    16441 gtttagcctc agccgttata ccactgaaga tgagatacag tatacgcttc aggtaatgcc
    16501 tgaggtagta aacaggttat tggcaatatc gccatattgg gatgcaatga ataaaaaagg
    16561 caagtccatg acagaacttg cccattgata gtataagatt attaaaaata tagtttaccg
    16621 tgcaacctca ttgataagcc ttcccagttc ctgatcgtgg gttagcattg ctttttgatt
    16681 ggcttcatag cttcgctgta cttcaatcat atccaccatc tcgcggacgg catttacatt
    16741 tgatttttca aggaatccct gtataacctt gtattcctcg gctggcaatg ccggtccaga
    16801 atattgtgtt tcatggtata atgattcgcc ttcttttttt aactcacgga tgttttcaaa
    16861 atcaacaatt tttagtttgt caataacaac cggctgctcc cagttattct gggttagccc
    16921 aatcatatca tcaggattta atgataatgc agagttaacc aggatttcac cccgttcatt
    16981 gatcataaaa ttgttcttct gtaattttat gatgccattt tgacccagga caggcaatcc
    17041 actatgcgtt accagtattc catctttatt cagggtaaaa gcgccattac gggtatatcg
    17101 ttcgccacgc tcggtcatga cagtgaagaa tccattaccc tccattgcca tgtcaaatgg
    17161 attttcagta cgcatcagcg agccctgttc atggatagta tatacctcgt tcacttcaac
    17221 accggtaccc aatttaccaa caaagggcat ggtatcatac gaaccagccg gagtaatacc
    17281 cagcccatca tcgttaaggc ggcggataat catatcaggg aatgctttaa aaagggtaag
    17341 gtctttttta tatgctgctt tatcaacatt tgccagatta ttggcaatag catcaagacg
    17401 tgcttcctgt gcaatcatac cgctggcacc tgtatagagt cctctcacca ttatggtttt
    17461 cctcctttta ctctaactat atcggcacct aacgcctgta acttttgctc aaaattttca
    17521 tatccacggt cggtatggta aatacggcgg atttcggttt ttcctctggc agctaacgca
    17581 gccagcacca gagaagcgcc tgcacgcaga tcggacatta aaaccggagc agcctgcaat
    17641 tgcttaccac ctttgataat agcagtattt ccttctattt caatgtcagc acccattcgc
    17701 cgtagttcgc caacatgggt ataacggttt tcaaaaatgg tttcaataat gacacttata
    17761 ccggggatag tacacataag cgacatgatt ggcgcctgaa ggtcggtagg gaagccaggg
    17821 tagggaagag tgcgtaccat actgccctgc aatttttttg acggttcaat ggtaacggag
    17881 ctatcgttac ctgaaataac aaaacccatg tcggcacagg tatcaataca ggccttaaca
    17941 tgttccggaa taacattatt aacggttaca ttaccacggg tgatagctcc tgcaattaaa
    18001 aaggtacctg cttctatgcg gtcaggtatt atggagtagc ttaccggctt cagttttttt
    18061 actccattga tgatgatgcg ctctgtcccc agtccttcaa tctttgctcc cattgttatt
    18121 aaaaaattgc ccagatcaac aacatcaggt tccatggcgc agttttcaag gattgtttgt
    18181 ccatctgcaa gcacagcggc catcatgatg ttggcagtgg cgccaacgga aaccttccgc
    18241 attataaaac gattgccgtt taatttttca acacgtgctt taatatagcc atgctcaatt
    18301 gttatggtgg ttcccagcgc ttcaaaaccc gaaagatgaa tatccaccgg acgctcgcca
    18361 attgcgcaac cgcccggcaa cgacacgtct gcctcgccgc atcgtgctaa aagcggaccc
    18421 ataacataga ttgaagcacg cattgttttc acaagatcat agggcacttc agcatttttt
    18481 aatgagttaa catgaatggt taaggtattg gtattgatgt caaactgata ttgtgcgccc
    18541 agacattcaa tgacctgtaa tattgtctga atgtccttta aattaggtac attgtgaaga
    18601 actgttgtac cttctgcaag caagcttgcg acaattattg gtaaagcggc atttttagct
    18661 ccggatatgg ttaccgtgcc attaagtttg ttgccgcctt tgactatata gaagtccaca
    18721 gttatgcctc tttttgtact ggtttagtga tatgctattg ataggttcag tatggatggt
    18781 ttgctaaatt gtcaattcaa ttcaacagtt attgataaaa aaattaacgc tattgtatga
    18841 aa
//
LOCUS       DAOOJH010000041        18800 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2901_length_18800_cov_10.190024, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000041 DAOOJH010000000
VERSION     DAOOJH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18800
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2901_length_18800_cov_10.190024"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..839)
                     /locus_tag="PLJ75_07010"
     CDS             complement(12..839)
                     /locus_tag="PLJ75_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05003.1"
                     /translation="MKTKFIRIDDVSEIDTSKIGIFDLANRYIDKYGNMYGLRYNRDV
                     KKIEVIKIMRTQKKHATYFSQKIQMRPEKPEAPHNDDYLGEEMEFDPEACINHIVELS
                     KTHKNRLSGIIMNIANSNIIADTDKMLWNTMNDLFRNIDMDAIQQLEKIADLYNEFNN
                     YPRSIIYYQSKLDTKHRKILDEIPAESSKFRYVKYYEMWYITKTTYKTIIKLLNDIRF
                     LLEQQHIEDKRNLTPFQKQTCSDALTSIDNTITEANTILADCGMVEELINTSKAFIG"
     gene            complement(853..1230)
                     /locus_tag="PLJ75_07015"
     CDS             complement(853..1230)
                     /locus_tag="PLJ75_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPD05004.1"
                     /translation="MTEKRIHPRKECNIKTKFDYFEGDPETVSVNQKPAKSSKGYILN
                     VSISGAFLVCNCRVSVQMPIKLYCKIQNTKLELEGEIVRTGMVQNNPSEVAQRYAAKN
                     VKGDSYIAIRFKELLPQSIIEQV"
     assembly_gap    1380..1479
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1480..2559
                     /locus_tag="PLJ75_07020"
     CDS             <1480..2559
                     /locus_tag="PLJ75_07020"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPD05005.1"
                     /translation="PTITVAGEQKTKPTQHSVKELMELGILPDILLCRSARTLSDELK
                     SKIALFCNVSERYVISAVDISHSIYEIPYMLHENQYDEVVLEHFGLPVKPLHIPAWDT
                     FFTSLMNPKKKVTIAVVGKYISLQDSYRSIYEALIHGAVANNAELEIQRIESEEIERK
                     YMNSLEELFKNVNGILIPGGFGTRGIEGKIATIEYARTHKIPLFGICLGLQCAVIEFA
                     RHGCGMENANSTEFDPTTPYPVISLLEEQELIDKLGGTMRLGAYLCMFKDNTRIKSIY
                     NTDSVMERHRHRFEFTLKYKQLFEKHGMIFSGFNPQSNLVETIEYADHPWFICTQFHP
                     EFKSKPVKPHPLFKDFIRASMENIC"
     gene            2681..4114
                     /gene="rpoN"
                     /locus_tag="PLJ75_07025"
     CDS             2681..4114
                     /gene="rpoN"
                     /locus_tag="PLJ75_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPD05006.1"
                     /translation="MALLKLDVVQTQRLRLTQTLRQSLDLLQISTLELEEILLQELQE
                     NPVLECDDALNEGFESLEDVAKNEMEGTVASKAEEFSIEYADASDTGIQNDFDKKNVL
                     EIVTRNESLQEHLLSQIRMLSLPQEDIDILEQLITSLDDNGFLTVSKEFIQNNYNIAS
                     EKLDSLLVVLNTVEPVGCGVFNVTESLMVQAQYYYPEDDLLQVIIKNYLPNIQRLEYD
                     RIAKNLNIRREEVKEKAILLTNLTPFPGALYLNSKPIYVRPDIEVQVIDDEVVIQLVD
                     NWIPRLYINAKYVQMLKQKKVDEKTRQFIHEKVSSAKNLITNIGRRNETLLLVAYAIM
                     NRQKEFLQKGQGFLKPLIYADLESELGFHESTIARAVANKYAQTKWGVFEFKYFFVKK
                     VKSEHNDETSQDTILKRIKELINSELKHNPLSDEEILDILRGEGIHIARRTIAKYRDM
                     LSIPASHIRKKLYLIKDEGNHEFNNHR"
     gene            4092..4901
                     /gene="raiA"
                     /locus_tag="PLJ75_07030"
     CDS             4092..4901
                     /gene="raiA"
                     /locus_tag="PLJ75_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPD05007.1"
                     /translation="MNLTITGRNFEVSDSIREYAEKKLTKLTKYFHQLIDMHIRLFIE
                     RQDHVAEITINGDGVQFYAKEKAGDMYSSIDLLLDKIEQQIVRYKERHSEHKVTPLNK
                     LEDIATYNVSGEQTPIVIRQVSNKPIDEKEAYLQMKLEGAEFMLFKKGEKSIKNAVDY
                     MNKNYAVIYKDGDTYTLIELPFEMIQNNNFDPSKFNVYSLDVKKESLINPEIKFIKKN
                     SCSVKIMSVDDAVVEITKSDVPFIVFFNSETHFLNIIYKKGKSLELLVPAF"
     gene            4911..5882
                     /gene="hprK"
                     /locus_tag="PLJ75_07035"
     CDS             4911..5882
                     /gene="hprK"
                     /locus_tag="PLJ75_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPD05008.1"
                     /translation="MKKYLRIHDILENTQKIDLQLTLVAGSEGLDKAITAIEINRPGL
                     TLAGFFDYFAYDRIQIFGLGETAYIKTLSRERREEIYHKFFSYDIVSCVYTHNEYPDE
                     IFIDFAKKYKVPTFVTRYPTTRFISLLTHIVEAAFAESVTIHGTLVDVFGIGILLIGK
                     SGVGKSETALELIERGHRLIADDLVEVKKIDESLLMGNGSELIRHHMEIRGIGILNVR
                     DIFGIRSVRNRKRIELVAELQEWDANTHYDRLGIEEQLYTILDLEVPYIIVPVRPGRN
                     IPIIIETAALNQRLKKIGIFSAQELDKKIQEWIVRENEDYEREKGEN"
     gene            5857..6132
                     /locus_tag="PLJ75_07040"
     CDS             5857..6132
                     /locus_tag="PLJ75_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPD05009.1"
                     /translation="MKEKKVKIKSDAGVHARPASILVREAMKFPCDIYLIKGQMEANG
                     KSIMSVLGLAITKGSFLTIRADGEMEEEAVNRLSALIENDFAELASK"
     gene            6129..6704
                     /locus_tag="PLJ75_07045"
     CDS             6129..6704
                     /locus_tag="PLJ75_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05010.1"
                     /translation="MKHCCSLSQYAIWILIAIMLLLPVNSFAQSKHKQELSLTDEEIK
                     TLFEKKKDPVFSGILSFYMPGLGQFYCDEKLKGAAFLITEYTIIIGSMFYFLDFNFKA
                     GGDSGFNLKVDANQTDMGLFSIERKHLFYVTVAALSLIHIFNIIDAVKSAYSFNSTLE
                     QKRKNLIQKYPQLDMGCNNDGGMYLAFSSHL"
     gene            6721..7248
                     /locus_tag="PLJ75_07050"
     CDS             6721..7248
                     /locus_tag="PLJ75_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05011.1"
                     /translation="MVLLCSTVVFAQNGKNVYKPLPLQEVPADVPIFFEEPERKDPFI
                     AGVLSWSWSGLGQFYTQNYKKGSAFLVADIAEKSLLVFMLFHISDKYSHGSEDVNWSD
                     IDKKDQYMVVGLLFSIFFTRVFSVVDAVNSAHDYNKNIYYPYWKSKQSFKTSFENTSS
                     NKSICLMLSHPVNFQ"
     gene            7245..7811
                     /locus_tag="PLJ75_07055"
     CDS             7245..7811
                     /locus_tag="PLJ75_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05012.1"
                     /translation="MKQLLCIITIVIVLMCSHTLFAQNVVYNDVRFDSTKLIVTISIT
                     DVKTYDILEALKRGLEGRIEYVVEIVENPVLPLLPKNVIKTSTVKKKVKFDFSKKAYI
                     VSRSNVPTLYYSDESIIEELFNNQQIVIDNAYTLRKSNYSIRIRVSFSSVKLYFPLNL
                     IFNYVVGIWDFDTGWEYGPKLVGIPYAE"
     gene            7835..9616
                     /locus_tag="PLJ75_07060"
     CDS             7835..9616
                     /locus_tag="PLJ75_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05013.1"
                     /translation="MKIHVKEYFKNRISRKNVKNSLIIIGGFFIFSVIIALLLPPRSE
                     SDSVGQGFFVNLVMIIPLAAAVFFIVLAFRLRVHPDGLYGGGSIRAKIALALIVIAIV
                     PTIPIIVITSNILNTTISQFFTEKTMIALERSVDMANEQILSIDNGLIRQLEQLRYDL
                     DSNFVSVANITNASFQKRYTFSQSYLYVTTIEHKGILNNRIQSLFLYPDRERVENDIN
                     TFLEIAKVTNAIACSRLAIDDQMYSVAYTQYGQYCIVMYRLIPRDLLSTSEFINTSFS
                     EYKKQEFLKPYFQTGIGIALIMISIGIILLAIVLSLILSQSITKPVYELVEAAEKIAK
                     GNFNINLVRREQDEMALLYESFNQMAKQLDQGKKMMYQTQKLKAWSDIARKLIHEIKN
                     PLTPIRLSAERLSRRYEEHHPDFSSILKEATNTIIEEVTILMNMLSEFSKFARLPEIN
                     LQAESINEIIENCIAMYYDNDKISFVLDLKPDIPPVMCDKTLLRQAFLNIIQNAIEAN
                     GHVITVSTAYDSQQGALTIAFSDDGVGIPDDEIEKVFEPTFSTKENGMGLGLAIVEKI
                     IIDHKGTITCSSTLKQGTKFTITIPQQ"
     gene            9627..10982
                     /locus_tag="PLJ75_07065"
     CDS             9627..10982
                     /locus_tag="PLJ75_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPD05014.1"
                     /translation="MAKILVVDDEKNILNTMKAILQDEGHVVYTVQNGKDALQFVKSN
                     ECDVVFLDVWLPDIDGMEVLQQIKQLQNDIAVIMISGHGSIDIAVKSTRLGAFDFLEK
                     PPSMERVITSLNNAIEQIQLKRENVILKKNITMEDEMIGNSIPMQEVKRIIDTAAATN
                     ARVFITGENGTGKELVARAIYRKSKRSDKPFIKVNCAAIPDELIESELFGHEKGSFTG
                     ALARRLGKFELADKGTIFLDEICDMSQSAQAKVLRVLQEQQFERVGGNDVITVDVRVI
                     AATNVDVKEAIEQGRFREDLYYRLNVIPIYVPPLSERKDDIPLLVDYFLEKFAREHGL
                     GVKQMSDSAMHFMVNYAWPGNVRELKNVIERLTIMVPSEVIELDDIKKHIESYDYEDT
                     IVKDSSSLKKAREQFEKEYIIKALKQFDKNVSATAKSLGIERTNLHRKIRQYHINVDK
                     L"
     gene            10986..11624
                     /locus_tag="PLJ75_07070"
     CDS             10986..11624
                     /locus_tag="PLJ75_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HPD05015.1"
                     /translation="MYSTLKEVLQTYRNYAHRLPMVIAYEDEVIPLTVNKAYHDTAQD
                     VQDTTATTVHEEIERCNQCQSTHKIAMQSRLTEAAIMIVLNPPALISSVEKKVLQPQV
                     DTMLQRMMKAIDVQMENCYITHMIKCESQKQLPGTMFLHCQQFLVKEINHVNPRVIIV
                     MGEMLPMRKIIKSMDAVQWFGIEHPITLIKNPELKKDAWKTLKLVRATLDEK"
     gene            11614..13566
                     /gene="priA"
                     /locus_tag="PLJ75_07075"
     CDS             11614..13566
                     /gene="priA"
                     /locus_tag="PLJ75_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPD05016.1"
                     /translation="MKNSDMFADVAVGLPVEGLFTYSTAEFPITKGMRVVVDFNNRKI
                     IGCVVNVHSQKPDFEIKPVAKIIDDEPIFDERLLAMAQFIAHHYVCYFGEAVSTTMPS
                     GKSYKTRQKTFFAGDSYSEITLTPEQEDIQQAILQQGQKAHCIYGITGSGKTEVYIAL
                     AREMIRRNQSVLYLVPEIGISSQMFMRLKEVFGNSLVMYHSGMSPNERLLSWRRFYHG
                     DAAIAVGTRSAIFMQAPHVGLIIIDEEHDASYKENSTPRYHARTVAWYRHKNEGSMLV
                     LGSATPSLETLYAVQGKRIMMHTLHRRFGNATLPQLEILPVKASGGNKIITNQLLMAI
                     KKTIEAKRQVILLLNRRGFAPRVICDDCGMSVQCPHCSISMTYHKKKLLCHYCHYEST
                     IPEQCEHCGSKNLIYVGSGTQRIEELLTRMFPLATIVRMDQDTARKKNFVAEVVSNMQ
                     QGKIDILLGTQMVAKGFDFPNVALVGVIMADIGLSLPDFRAAERIFALLVQVAGRSGR
                     GDIAGKVIVQTINPDNPLFNHLKNYDYIGFCQRELSYRKALNYPPYVRLARLVIRGKD
                     EDEVAQVATLYKKKLDELLAHYPGITILGPSQAPLYKIASYFRYHIVLKSGSVDALSG
                     MIAQCKKNMDVHNAYIEVDIDPLDLM"
     gene            complement(13571..14068)
                     /locus_tag="PLJ75_07080"
     CDS             complement(13571..14068)
                     /locus_tag="PLJ75_07080"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HPD05017.1"
                     /translation="MPEFPEIPDEIKKLFEEHAIDEIRLDAEGNWFHNGEPFTNQRII
                     RFFNSSITITRDGTYVLHYANFTYPIVVEDAPIFVTGARFEGFADFEHVILTLSSGEE
                     EELSIDTLHIRKAAELYCYVRNNTMLARFKRSAAFTILERLEESDDIFYVTIAGNKIV
                     LEEKK"
     gene            complement(14328..16394)
                     /locus_tag="PLJ75_07085"
     CDS             complement(14328..16394)
                     /locus_tag="PLJ75_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05018.1"
                     /translation="MPPVKKNRFTIIPEDSHTQGMRILSIEEENKEEKKSKKLHLSIP
                     KQLHPLIAAMVLIAGVALVVGIVSLVKKPESFLSSIQKSGNNAYKLLPGLSDEKLAPE
                     DISQNQHILRGKTYYFKKNYTSAIDEFNQVVESDASDNDKALALTYMGMIYDDKGDYN
                     KAIETLKRALKYKPDDTTVLKNLALAYRHKKDYPDALATITRAIAIDGNNPDLLLLQG
                     NIYFDMGRFPDAAKSYTKALDIAPDNPQALYNTGLTFLKTGDELSATEYFKKAAAADP
                     TGAIAKSAYSRLGSFYTNRRDYAMAEKYLNLAIQLDPNEPINHYNLGIVYMQRQQFDK
                     ALAEFKAAESSGIYEEELLEGLSQAYASLDRYDEGIAVLERLRPVENRNVAIIARLAE
                     LYYRKGDLDTAFEYYKQITIYEPVSENARVAYANMGNIRDDQHRFDEAVAYYKKALAI
                     DPNDSSTLYNMGIAYKHAGKPELAVAAFNEASRNNPDNPKPRLAIANLYYEKGYLDLA
                     LDEYIKVVRLWPHLQEAQFMLGMLYHSKGRDDYALEAFNRVIAINQNNDYAKKAYVNT
                     GIILSTSKDEKQQAQAIQSLQKALLIAPNDAEALLALGTIAFNQHKYDKAMEIFYQVL
                     EVSNDDAIVAQAYHTIGRCYYAKHDYKKSLQFLTKASELDPSNEQIRLNRKVATEAYE
                     KELSKK"
     gene            complement(16400..16813)
                     /gene="nusB"
                     /locus_tag="PLJ75_07090"
     CDS             complement(16400..16813)
                     /gene="nusB"
                     /locus_tag="PLJ75_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPD05019.1"
                     /translation="MGHRRKAREFAMQALYMYETVGTPVDELTRLDWVDKEIPEDIMD
                     FALNLITGTIANIDCIDNLIKQYSRNWKFERINPVDKSILRISVYALLYCDGIPHAVT
                     IDEGIELGKIYGSENSGQFINGILDAIRIHEGEKK"
     gene            complement(16813..17274)
                     /gene="ribE"
                     /locus_tag="PLJ75_07095"
     CDS             complement(16813..17274)
                     /gene="ribE"
                     /locus_tag="PLJ75_07095"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPD05020.1"
                     /translation="MNTIEGKLDASGLTIAIIVSRFNEFITSKLLSGALDCLQRHNAD
                     EKNICVVWTPGAFEIPPIAMKLSKGNYDAVVCLGAVIRGATPHFDYVAAEVSKGVAQV
                     SLQSNIPVIYGVLTTDTIEQAVERAGTKSGNKGFDAAMAAIEMAQLYKQLR"
     gene            complement(17519..18283)
                     /locus_tag="PLJ75_07100"
     CDS             complement(17519..18283)
                     /locus_tag="PLJ75_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPD05021.1"
                     /translation="MITKKVEDNVYILTLNDGKTNALTDTVLNDLRKYLKEAASDATI
                     KGVILTGEGKTFSSGFHLPMFLSFTTINEVIKFFDVEEEILLELFMFEKPVIAALNGH
                     TVAGGMIFAMACDYRIMKNHPKIKMGMSEIKIGLPLSVAQWNVVRFGLDSDKKFRDIM
                     YFGNMFSPDEALKMGLVDELADEDKLISRAKELINLWKNNPGNAFTPLKLCVRQETAD
                     RIRKDLTDGSWTQGLQCFFKDDVRKTLEFVQATMSK"
BASE COUNT         5778 a         3296 c         3797 g         5829 t
ORIGIN      
        1 ggttgtgatt attaaccaat aaatgcttta ctggtattta taagctcttc aaccatgccg
       61 cagtcagcaa gaatggtatt agcttcggtg atagtattat caatagatgt taaagcatca
      121 gagcaggtct gtttttgaaa tggggtgaga tttcttttat cttctatatg ctgttgttca
      181 agcaaaaatc gtatatcatt gagcagtttg atgattgttt tatatgttgt ttttgttata
      241 taccacatct catagtactt aacgtaacgg aattttgacg attctgcagg aatttcatcc
      301 aggattttgc gatgttttgt atctaatttt gattgatagt agatgattga gcgaggataa
      361 ttattaaatt cgttatacag gtcggcaatc ttttctaact gctgaatggc atccatatct
      421 atattcctga ataaatcgtt catggtattc cataacatct tatcggtatc agcaataatg
      481 tttgaatttg caatgttcat gataatgccg ctaagacggt ttttatgtgt tttactaagt
      541 tcaacgatat ggttaataca tgcttcagga tcaaactcca tttcctcgcc cagatagtca
      601 tcattatgag gggcttctgg tttttcaggt cgcatttgta ttttttgtga aaaataggta
      661 gcatgttttt tttgtgtacg catgatcttt attacttcta tcttttttac atcacgatta
      721 taccgcaatc catacatgtt accatatttg tcgatgtaac gattggccag atcaaatata
      781 ccaattttag atgtgtcgat ttcggataca tcgtcgatac ggataaattt agttttcatt
      841 gtacctgcta tgttatacct gctcaatgat tgattgaggt aaaagctctt taaagcgtat
      901 agctatataa ctatcgccct taacattttt tgctgcatag cgttgcgcta cctcagaagg
      961 attgttttgc accatccctg ttcgtacaat ttcgccctct aattctaatt ttgtgttttg
     1021 tattttacaa taaagcttaa ttggcatctg tacactgaca cgacaattac aaactaaaaa
     1081 ggctcctgaa atacttacat tcagtatata cccttttgaa cttttggctg gcttttgatt
     1141 tacgcttact gtttcaggat ctccctcaaa gtaatcaaat tttgttttaa tattgcattc
     1201 ttttcgtggg tgaattcttt tttcggtcat ggtattgtcc tattgaataa aaattaataa
     1261 attttcttta cagaagtttg gattgtaagt atagtatctt tgaatgtttt gtcaagaata
     1321 gttttattat acgctaaaat ttgcgatagt tactacaaat tcttgacata tgcatggcan
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctactattac cgtagcaggc
     1501 gaacagaaaa caaagccaac ccagcattca gtaaaagagc ttatggaatt gggaatacta
     1561 ccggatattc ttctttgcag gtctgcccga acgttaagcg atgaattaaa aagcaaaatt
     1621 gcattatttt gtaatgtatc ggaacgttat gttatttcag cagttgacat cagtcactca
     1681 atctatgaga taccatatat gctgcatgaa aatcagtatg atgaggttgt actggaacat
     1741 tttggtttgc ctgttaaacc actgcatatc cccgcgtggg atacgttttt tacttcgttg
     1801 atgaatccta agaaaaaggt tacgattgct gtagttggca agtatatctc gttacaggat
     1861 tcataccgct ctatatatga agctttaatc catggggctg tggcaaataa tgcggaactg
     1921 gaaatacaaa ggattgagtc ggaagagata gaacgtaaat acatgaattc actggaagag
     1981 ctttttaaaa atgttaatgg tatattaata cctggtggtt ttggtaccag gggaattgaa
     2041 ggtaaaattg ccacgattga atatgcccga acacataaaa taccgctgtt tggtatttgt
     2101 ttagggttgc aatgtgctgt tattgaattt gctcgtcatg gatgtggcat ggaaaacgca
     2161 aattcaactg aatttgatcc caccacacct tatccggtta tctcccttct tgaagaacag
     2221 gagttgatcg ataaattagg cggcaccatg cggctgggag cttatttatg tatgtttaaa
     2281 gataatacca gaattaaatc aatctataat actgattcgg ttatggaacg tcaccgccat
     2341 agatttgagt ttacgctaaa atataaacaa ctatttgaaa agcatggcat gatattctca
     2401 gggtttaatc ctcaaagcaa ccttgtagaa actattgaat atgctgatca tccatggttt
     2461 atttgtactc agtttcatcc tgaatttaaa tcaaaacctg taaagccgca tccattgttt
     2521 aaagatttta tacgtgcatc catggaaaat atttgttaaa tatgcaattt aaatagacgt
     2581 tgccaatact atttttgtat atttatacgt aggcaaagaa atagaatatg tgaaaaagaa
     2641 tttaacattt gaattgaagg cgattagtgc gaggaaatga atggctttat taaagcttga
     2701 tgtagtacaa acccagcgtt tacgtcttac tcaaactctt agacaatcat tagatttatt
     2761 acagatttcc actcttgaac tggaggagat attattgcag gagttgcagg aaaatccagt
     2821 tttagaatgt gatgatgctc tcaatgaagg atttgaatcg cttgaagatg tcgctaaaaa
     2881 tgaaatggaa ggtactgttg catcaaaagc tgaggagttc tctattgaat acgctgatgc
     2941 aagtgatacc ggtatacaaa atgattttga taaaaaaaat gttttggaga tagttaccag
     3001 gaatgagtca ttgcaggaac atctgttatc acagatacgg atgctgtcat tgccgcagga
     3061 agatattgat atattggagc aattgattac aagtctggat gataatggtt ttttaacagt
     3121 ttcaaaagag tttatccaga ataattacaa tattgcttca gaaaaattag acagtcttct
     3181 tgttgtactc aatactgtgg agcctgttgg gtgtggggtt ttcaatgtta cggaatcgct
     3241 catggtgcag gcgcagtatt actatccaga ggatgatctg ttgcaggtga ttatcaagaa
     3301 ttatttacca aacatacagc gccttgaata tgacaggata gcaaaaaatt tgaatattcg
     3361 cagagaggaa gttaaagaaa aagcaatatt gctgacaaat ctaacacctt ttcccggtgc
     3421 actctattta aatagcaaac ctatctatgt tcgtcctgat atagaggttc aggtgattga
     3481 tgatgaagta gttatacaac tggtggataa ttggattccc agactttata tcaatgctaa
     3541 gtatgtccag atgttgaaac aaaaaaaagt tgatgaaaaa acacgtcaat ttatacatga
     3601 aaaggttagt tcagcaaaaa atttaatcac gaatattgga aggcggaatg aaactttatt
     3661 gttagttgct tacgctatta tgaacaggca aaaagagttt ttgcaaaagg ggcagggttt
     3721 tttaaaaccg ttgatttatg cagatttaga atcagaattg ggctttcatg aatcaacaat
     3781 tgcccgtgct gttgctaata aatatgctca aacaaaatgg ggcgtttttg aatttaaata
     3841 tttttttgta aagaaggtta aatctgaaca taacgatgaa acatcacagg atacaatatt
     3901 aaagcgcatc aaagagctca tcaatagtga gctgaaacat aatcccttga gtgatgagga
     3961 aatacttgac atcttaaggg gtgaaggtat tcatattgct cgtagaacta ttgcaaaata
     4021 cagggatatg ttgtctatcc ctgcatcaca tattcgtaaa aaattatatt tgattaagga
     4081 tgagggtaac catgaattta acaatcacag gtagaaattt tgaagtgtct gatagcatac
     4141 gcgaatatgc tgagaaaaag ctaacaaaac ttacaaagta ttttcatcag ttgattgata
     4201 tgcacatacg cttgttcatt gagcggcagg atcatgtggc agaaattaca attaatggtg
     4261 atggtgtgca gttttatgca aaggaaaaag caggagatat gtattcctcc atagatcttt
     4321 tgcttgataa gattgagcaa caaattgtgc gatataaaga acgacattca gaacataaag
     4381 taaccccatt aaacaaatta gaggatattg ctacgtataa tgtcagtggt gaacaaacac
     4441 caatagttat acgccaggtt agcaataagc caattgatga aaaagaagca tatttgcaga
     4501 tgaaattaga aggtgccgaa ttcatgcttt tcaagaaggg agaaaaatca ataaagaatg
     4561 ctgttgatta tatgaataaa aactatgctg ttatttataa agatggagat acctacacat
     4621 taatagaact cccttttgag atgatacaaa ataacaattt tgatccatct aaattcaatg
     4681 tatattcact ggatgttaag aaagaatcat tgatcaatcc tgagattaaa tttataaaga
     4741 aaaattcatg ctctgttaaa attatgtcag tagatgatgc agttgtagaa ataactaaaa
     4801 gtgatgtccc gttcatagta ttttttaact ctgaaacaca ttttttaaat attatttata
     4861 aaaaaggaaa atcacttgaa ttattagtgc cagcatttta gaaaagagaa atgaaaaagt
     4921 atctacgcat tcatgatatc ttagaaaata ctcagaagat agatcttcag cttacgcttg
     4981 ttgctggcag tgaaggttta gataaggcta taacagcgat agaaattaac cggccggggc
     5041 ttaccctggc tggctttttt gattattttg cttatgatcg cattcagata tttggtctgg
     5101 gtgaaactgc atatatcaaa acattatccc gagaacgacg cgaggagata taccataaat
     5161 tcttctcgta tgatatagtc agttgtgtat atacacacaa tgaatatccg gatgaaatat
     5221 ttatagattt tgcaaaaaaa tataaagtcc caacatttgt aaccagatat ccaacaacac
     5281 gatttataag tttgctaacg catattgttg aggctgcatt tgctgaatcg gttactatcc
     5341 atggtacact ggtagatgtc tttggaattg gtatactcct aattggtaaa agtggtgttg
     5401 gtaaaagcga aacagcgctt gagctcattg aacgtgggca tcgcttaatt gcagatgatc
     5461 tggtagaggt taaaaagatt gacgaatcgt tgcttatggg aaacggttct gaactgatac
     5521 gccatcatat ggaaatacgg ggtattggaa ttttaaatgt gcgtgatata tttggtatac
     5581 gatcagtacg aaaccgtaag cgtattgagc ttgttgctga actgcaggaa tgggatgcaa
     5641 atacccatta tgatagattg ggaattgaag agcagttata tactattctt gatcttgaag
     5701 ttccctatat tattgtacct gtaagaccgg gaaggaatat accgataatt attgaaacgg
     5761 ctgcgcttaa tcagcggctt aaaaaaatag ggatattttc cgctcaggaa cttgataaaa
     5821 agattcagga atggattgta cgggagaatg aagattatga aagagaaaaa ggtgaaaatt
     5881 aaaagtgatg ctggtgtcca tgcgcggccc gcttcaatac ttgtacgtga agcaatgaag
     5941 ttcccctgtg atatttattt aattaaagga caaatggaag caaatgggaa atcaatcatg
     6001 tctgtgttag ggctggctat cacaaaggga agcttcctga caatacgtgc tgatggtgaa
     6061 atggaagaag aagcagttaa caggttatcg gcattaattg aaaatgattt tgcagagctg
     6121 gcttcaaaat gaaacattgt tgctcattat cacaatatgc tatctggatt ctcattgcaa
     6181 taatgctgct tttgccagtg aatagttttg ctcagtcaaa acataaacag gaactatcgc
     6241 ttactgatga agaaataaaa acattatttg aaaaaaagaa agaccctgtt ttttcaggta
     6301 tactttcatt ttatatgccc gggcttggcc agttttattg tgatgaaaag ctaaaagggg
     6361 ctgcattcct tattactgaa tacactatta ttattgggtc gatgttttat tttctggatt
     6421 ttaacttcaa agcaggtggt gattccggat ttaaccttaa agttgatgca aaccaaacgg
     6481 atatgggttt attcagtatt gagaggaagc atttatttta tgtaacagtt gcagcactat
     6541 cactgatcca tatttttaat atcatagatg ccgtgaagag tgcttattca tttaatagca
     6601 cactggagca aaaacgaaag aaccttattc aaaaatatcc acaacttgat atgggatgta
     6661 acaatgatgg aggtatgtat cttgcttttt cctcgcattt ataggataac tacaacctgt
     6721 atggtacttc tgtgctctac agtagttttt gcacaaaatg gaaaaaatgt ttataagcca
     6781 ttgccgcttc aggaagtacc cgctgatgtg ccaatttttt ttgaagagcc tgaacggaaa
     6841 gaccctttta ttgccggtgt tttatcatgg tcgtggagcg gtttaggaca attttatact
     6901 caaaattata agaaaggttc ggcgtttttg gttgccgata ttgccgaaaa aagtttactg
     6961 gttttcatgt tatttcatat atctgataaa tattcgcatg gttccgaaga tgtaaactgg
     7021 tcggatattg ataaaaaaga tcaatatatg gttgtggggc tattgttttc aatatttttt
     7081 acaagagtgt tttctgtagt tgatgctgta aattcagctc atgactataa taaaaatatt
     7141 tattatccgt actggaaaag caaacaaagt tttaagacat cctttgaaaa caccagtagc
     7201 aataaatcta tatgtttgat gctatcacat ccagttaatt tccaatgaag caattacttt
     7261 gtattatcac cattgttatt gtgttaatgt gctcgcacac attgtttgca cagaatgtgg
     7321 tatacaatga tgtgcgattt gattcaacta agctcatagt taccatttca attaccgatg
     7381 ttaaaaccta tgatatactg gaagccttaa agcgaggatt ggaagggagg attgaatatg
     7441 ttgttgagat tgttgaaaat cctgtactgc ctcttttacc caaaaatgta ataaaaacaa
     7501 gtacggttaa gaaaaaagtt aaatttgatt tttctaaaaa ggcatatatt gtgtcccgat
     7561 caaatgttcc cacactgtat tatagtgatg aatcaattat tgaagaactt ttcaataatc
     7621 agcagatagt tattgacaat gcctatacct tacggaaatc aaactattct atacgtattc
     7681 gtgtttcatt tagttcggta aaactatatt ttccccttaa cctgatattt aattatgttg
     7741 taggtatatg ggattttgat acaggctggg aatatggtcc aaaacttgtt ggcatacctt
     7801 acgctgaata acggtatggt atggaaatga atttatgaaa attcatgtaa aagaatattt
     7861 taaaaatcgt atatccagaa aaaatgtaaa gaattcattg attattattg gcggcttttt
     7921 tatcttttct gtgatcatag cgcttctttt acccccgcgc agtgaaagtg attcagtggg
     7981 tcagggattt tttgttaatc ttgttatgat tataccgctg gctgctgctg tattttttat
     8041 tgtgctggca ttcaggctca gggtgcatcc tgatggtctt tacggtggcg gcagtatacg
     8101 agcaaagata gcattagctc ttattgttat agcgatagtc ccaacgatac caatcatcgt
     8161 tattaccagc aacattttga acactaccat cagtcagttt tttacagaaa aaacaatgat
     8221 agcccttgaa cgatctgttg atatggcaaa tgagcaaata ctgtcgattg acaatggttt
     8281 aatacgacaa ttagaacaac tcaggtacga tcttgatagt aattttgtaa gtgtggctaa
     8341 tattaccaat gcttcatttc aaaaacggta tacattttcg caatcctatc tatatgtaac
     8401 aactatcgaa cataaaggta ttttgaacaa ccgcatacaa tcattgtttc tatacccaga
     8461 cagggaacgc gttgaaaatg acattaatac ttttctggaa atagcaaagg taacaaacgc
     8521 aatagcatgc agcagattgg caattgatga tcaaatgtat tcagtggcat atacacaata
     8581 tggtcagtat tgcattgtaa tgtatcgatt gataccaagg gatttacttt ctacttctga
     8641 gtttatcaat acttcattta gtgaatataa aaagcaggaa tttttaaaac cgtatttcca
     8701 aaccggcatt ggaattgccc ttatcatgat atcaattggg attatattgc ttgcgatagt
     8761 tcttagttta atactatctc agagcattac aaagcctgta tatgagctgg ttgaagccgc
     8821 tgaaaagatt gcaaagggta attttaacat aaatctggta cgcagggaac aggatgagat
     8881 ggcattgctc tacgaatcat ttaaccagat ggcaaaacaa ctggatcagg gtaagaagat
     8941 gatgtatcaa acacagaaat tgaaggcatg gagcgatata gcacgaaagc tcatccacga
     9001 aattaaaaat cccctgacac ctatacggtt atcggcagag cggttgtccc gacggtatga
     9061 agaacatcat ccagattttt cctccatcct taaagaagca acaaatacaa ttatagagga
     9121 agtaacaata cttatgaata tgctttcgga gttttcaaaa tttgcaaggc ttccggagat
     9181 aaacctgcaa gcggagagta ttaacgagat tattgaaaat tgtattgcga tgtattacga
     9241 taatgataaa atttcatttg tgcttgactt aaaacctgat ataccacctg ttatgtgtga
     9301 taaaacatta ttacggcagg cattcttaaa tataattcag aatgctattg aagcaaatgg
     9361 acatgtaata acagtatcta ctgcttatga tagtcaacag ggagcgttaa ccattgcttt
     9421 tagtgatgat ggtgttggca ttccggatga cgaaattgaa aaggtttttg aaccaacgtt
     9481 ttctactaaa gaaaatggta tgggattggg gcttgctatt gtggaaaaga ttattattga
     9541 tcataagggc actatcacct gcagttccac attaaaacag gggactaaat ttaccataac
     9601 tatcccccag caataaggtt ataactatgg caaagatttt agttgttgat gatgaaaaaa
     9661 atatattaaa caccatgaag gcaatcctgc aggatgaagg ccatgtggtg tatactgtcc
     9721 agaatggaaa agatgctctc cagtttgtaa aatccaatga atgtgatgtg gtttttttag
     9781 atgtatggct tcctgatatt gacgggatgg aggtcttaca gcagataaaa cagttgcaga
     9841 atgatatcgc agtcatcatg atatcaggtc atggttcaat tgatattgca gtaaaatcta
     9901 cacggcttgg tgcttttgat tttctggaaa agccaccttc catggaacgt gttataacct
     9961 cgctgaacaa cgccattgag cagatacagc ttaagcgtga aaatgttata ttaaaaaaga
    10021 acatcaccat ggaagatgag atgattggca attccatccc aatgcaggag gttaaacgca
    10081 tcattgatac tgctgctgca accaatgctc gtgtttttat aacgggtgaa aatggcacag
    10141 gcaaggaact ggtagcacga gctatctacc gaaaatcaaa acgaagtgat aaaccattca
    10201 taaaggttaa ctgtgcagcg atccctgatg aactcattga aagcgagttg tttggccatg
    10261 aaaagggttc atttaccggt gcacttgccc ggcggttggg aaaatttgaa ctggctgata
    10321 agggtacaat ctttcttgat gaaatttgcg atatgagtca gagcgctcag gctaaagtat
    10381 tgcgtgtatt gcaggagcaa cagtttgagc gagtaggcgg caatgacgtt ataaccgttg
    10441 atgtgcgggt tattgctgca accaatgttg atgtaaaaga ggctattgag cagggacgtt
    10501 ttagagagga tttatattac cggttaaatg taattcccat ttatgtccct ccactttctg
    10561 agcgcaagga cgatataccg cttttagtgg attattttct tgaaaagttt gctcgtgaac
    10621 atggtctggg cgttaaacag atgagcgaca gcgctatgca ttttatggta aactatgcat
    10681 ggccgggcaa tgtgcgcgaa cttaaaaatg tcattgaacg gcttaccata atggtgccat
    10741 cagaggttat tgagcttgat gatataaaga agcatattga atcgtatgat tacgaagata
    10801 ccatagtgaa ggattcatca agtttaaaga aagctcgaga gcaatttgaa aaagagtata
    10861 ttataaaggc attaaaacag tttgataaaa acgtatcagc aaccgccaaa tcattgggga
    10921 ttgagcgcac caatcttcac aggaagatac ggcagtatca tatcaatgtt gataagttat
    10981 agagtatgta cagcacatta aaagaagtat tgcaaaccta tcgtaactat gctcaccgtt
    11041 tacccatggt aatagcatac gaagatgaag taattccttt gacagttaac aaagcgtatc
    11101 atgacactgc gcaggatgtg caggatacaa cagctactac cgtacatgaa gagattgaac
    11161 ggtgtaatca atgtcaatcc acgcataaga ttgccatgca gagcagattg actgaagcgg
    11221 ctatcatgat tgtgctaaat ccacctgcat taatttcctc agttgaaaaa aaggtattgc
    11281 agcctcaggt tgataccatg cttcagcgca tgatgaaagc cattgatgtc caaatggaaa
    11341 actgttacat tacccacatg attaaatgtg aaagccagaa acaattaccc ggaacgatgt
    11401 ttttgcactg tcagcaattt cttgttaaag agataaatca tgttaatcca cgcgtcatta
    11461 tagttatggg cgaaatgctc cccatgcgga aaataataaa aagcatggat gccgttcaat
    11521 ggtttggcat agagcaccca ataacattaa ttaaaaatcc tgaattaaaa aaagatgcat
    11581 ggaaaaccct caaattagtc agggcaacat tggatgaaaa atagcgatat gtttgctgat
    11641 gttgccgttg gattgcctgt tgaaggtttg tttacctata gcacagcaga gttcccgatc
    11701 acaaaaggga tgcgggttgt cgttgacttc aacaatagaa aaataatcgg ttgtgttgtc
    11761 aatgttcatt cacaaaaacc tgattttgaa attaagcctg ttgcaaaaat aattgacgat
    11821 gaaccaatct ttgatgagcg cctgcttgct atggcgcaat ttattgcgca tcactatgta
    11881 tgttattttg gcgaggctgt gagcactacc atgccatccg gaaaatcgta taaaacaaga
    11941 caaaagactt tttttgcggg cgatagttac tctgaaatta cccttacccc tgaacaggaa
    12001 gacatacagc aggcaatact gcagcaaggg caaaaggcac actgcatcta tggtataacc
    12061 ggaagcggca aaacagaggt ttacattgca ttagccaggg agatgattag gcgcaatcaa
    12121 tcggtgttat acctggtgcc cgagattggt atatcatcgc agatgtttat gcgtcttaag
    12181 gaagtatttg gcaacagcct tgtgatgtat cacagcggca tgagtcctaa tgagcggttg
    12241 ttaagctggc ggcgctttta ccatggggat gctgcgattg ctgttggtac tcgctcggct
    12301 atatttatgc aggcaccaca tgttgggctt atcattatcg atgaggagca cgatgcctcg
    12361 tataaagaaa acagtacgcc acgctatcat gcacgcaccg ttgcatggta ccgccataaa
    12421 aatgaaggca gcatgcttgt attgggttcg gcaactccat cactggaaac gctgtacgca
    12481 gtgcagggaa aaagaataat gatgcataca ttgcacaggc gttttggcaa tgcaacactg
    12541 ccacagcttg aaatactgcc tgtaaaagct tcaggtggca ataaaataat taccaatcaa
    12601 ctgctgatgg caattaaaaa gacaattgaa gcaaaaagac aggttatact actgcttaac
    12661 aggcggggat ttgcacctcg tgttatatgt gatgattgcg gcatgagcgt acagtgtcct
    12721 cattgcagca taagcatgac ctatcataaa aaaaaattgt tatgccatta ttgccattat
    12781 gagtccacca tccctgagca gtgtgaacac tgcggttcaa aaaatttaat atatgttggc
    12841 agcggcactc agcgcataga agaactatta accaggatgt tccctctggc tactattgtg
    12901 cgcatggatc aggatactgc acgaaagaag aactttgttg ctgaggttgt cagcaacatg
    12961 cagcagggga agattgatat tctgcttggt actcagatgg ttgcaaaggg atttgatttt
    13021 cccaatgtgg cactggttgg cgttattatg gcagatattg ggttatcgct tccggatttc
    13081 cgagcagcag aacgcatctt tgcattgctg gtacaggttg caggaaggtc gggcaggggc
    13141 gatattgcag gtaaagtcat agtgcaaaca attaatcctg acaatccgct ttttaatcat
    13201 cttaaaaatt atgactacat tggtttttgt caaagggaac tatcgtaccg aaaagccttg
    13261 aactatcctc cgtatgtacg gctggcacgg ctggttatcc gtggtaaaga tgaagatgag
    13321 gtggcacagg ttgcaacgct ttataaaaaa aagctggatg agctgttagc gcactatcct
    13381 ggcatcacta ttttaggacc gtcacaggca ccgctgtata aaatagcttc atacttccgc
    13441 tatcacattg tattgaaatc aggcagcgtt gatgcattaa gtgggatgat agcacaatgc
    13501 aaaaaaaata tggatgtgca taacgcctat atagaagtag atattgatcc acttgattta
    13561 atgtaagcat ttattttttt tcttcaagga caattttatt gccggcgata gttacataaa
    13621 aaatgtcatc actttcttca agcctttcca gtatggtaaa agctgctgag cgtttaaacc
    13681 ttgcaagcat ggtgttgttg cgaacatagc agtaaagctc agcagctttt ctgatatgaa
    13741 gcgtgtcaat agaaagttcc tcttcctcgc cgctgctcaa tgtaaggata acatgttcaa
    13801 agtcagcaaa gccttcaaag cgtgcaccgg taacaaagat aggtgcgtcc tctacaacga
    13861 taggataggt aaaattggcg taatgcaata cgtatgttcc atcgcgtgta atagtaattg
    13921 atgaattaaa aaaacgtata atacgctgat tggtaaaagg ctcgccattg tgaaaccagt
    13981 tgccttcagc atcaaggcgt atttcatcaa ttgcatgctc ttcaaataat ttttttattt
    14041 catcagggat ttcaggaaac tctggcatac agtatattca cctctcaaaa aagatagtgt
    14101 atagtatcac tggctgtgta aggttaccta ttttgtatca tgattatatg ccatggtagg
    14161 tacatacatg ctcgtgtgca aacacatttt tcttttatac tgccatcttt aagggagttg
    14221 ggtagtattg tattgctgat tcaccgaaat agaagtatac acctacacaa cccccttgtg
    14281 ttcccccctt tgtaaaaggt gggaattgtg tggaggttaa atcctcatta tttcttcgac
    14341 agctcttttt catacgcttc cgttgctact ttacggttta agcgtatctg ctcgtttgaa
    14401 gggtctaact ccgaagcttt tgttaaaaat tgtaatgatt ttttatagtc atgctttgca
    14461 tagtaacacc tgcctatagt atggtaggct tgcgcaacaa ttgcgtcgtc attggatacc
    14521 tccagcacct gatagaatat ctccatagcc ttatcgtatt tatgctggtt gaaggcaatt
    14581 gttccaagtg ctaacaatgc ttccgcatca ttgggagcaa tgagcaatgc cttttgcaac
    14641 gattgtatag cctgtgcctg ctgtttttca tcttttgatg ttgataaaat aatccctgtg
    14701 ttaacatagg ctttttttgc atagtcattg ttttgattta ttgcaatgac cctgttaaat
    14761 gcttccagtg catagtcatc tctgcctttg gagtggtaca gcataccaag cataaactgg
    14821 gcttcctgta aatgaggcca caggcgaaca acctttatgt attcatccag cgctaaatca
    14881 agatacccct tttcataata tagatttgca attgcaaggc gtggttttgg attatcagga
    14941 ttattccggg aagcttcgtt aaatgctgca actgccagtt caggcttacc ggcatgttta
    15001 taggcaatgc ccatattata cagggtggag ctatcgtttg ggtctattgc cagtgctttt
    15061 ttataatagg caacagcttc atcaaagcga tgctggtcgt cacgaatgtt tcccatattg
    15121 gcgtaggcaa cgcgtgcatt ttctgaaacg ggttcataga tggtaatttg cttatagtac
    15181 tcaaacgcag tatccagatc ccctttacga tagtacagct cggctaaacg tgcaatgatc
    15241 gcaacattcc tgttttcaac cggacgtaaa cgctctaaaa ctgcaatacc ttcatcatag
    15301 cgatccaggg atgcatacgc ctgcgataag ccttctaaaa gttcttcttc atagatgccg
    15361 gatgattcgg cagctttaaa ttctgccagt gctttgtcaa attgctggcg ctgcatataa
    15421 acaataccca gattgtagtg gttgattggt tcattggggt caagctgtat ggcaaggttt
    15481 aaatattttt cggccatggc atagtccctg cggttggtgt aaaacgagcc taaacggcta
    15541 tatgctgatt ttgcaatagc accggtaggg tcagcagcgg cagctttttt aaaatattcg
    15601 gttgcagata gctcatcgcc ggtttttaaa aatgttaatc cggtgttata gagggcttgt
    15661 ggattatccg gtgcaatatc cagagccttt gtataacttt ttgcagcatc agggaatctg
    15721 cccatatcaa aataaatatt tccctgtaat aatagtaaat ccggattatt gccatcaatg
    15781 gcgatagctc tggtaatagt ggctaaagcg tcgggatagt cttttttatg gcgatacgct
    15841 agtgctaaat ttttaagtac ggtggtatca tcaggtttgt actttaatgc tcgtttcaat
    15901 gtttcaatag ctttattgta gtcaccctta tcatcgtata tcatgcccat gtaggtaagt
    15961 gctaaagcct tatcattatc agatgcatcc gattcaacta cctgattaaa ctcatcaatg
    16021 gcgctggtat aatttttttt aaaatagtat gttttcccgc gcagtatatg ctgattttgt
    16081 gatatgtcct caggggcaag cttttcatcg cttaagccgg gtaaaagttt ataggcattg
    16141 ttgcctgatt tttgaatgct ggataaaaat gattctggct tctttacaag cgatacaata
    16201 ccaacaacaa gagcaacgcc tgcaatgagc accattgcgg caataagggg atgaagttgt
    16261 tttggtatgc tgagatgtaa ttttttgctc tttttttctt ctttattttc ttcttcaatg
    16321 gaaaggatac gcatcccctg tgtatggcta tcttcaggaa taatggtgaa tctatttttt
    16381 tttaccggag gcatggtatt tacttctttt caccttcatg aatccgtatg gcatctaaaa
    16441 tcccatttat aaattgtccc gaattttcgc taccataaat cttgcccaat tcaatgcctt
    16501 catcaatagt caccgcatgc ggtatcccat cacaatagag cagagcatat accgagatgc
    16561 gcaatatgga tttatcaacc ggatttatgc gttcaaattt ccagtttcgg gaatactgct
    16621 taattaaatt atcaatacaa tcaatattgg cgatagttcc ggtaataaga tttaatgcaa
    16681 aatccatgat atcttcaggg atttctttat caacccaatc aagccgtgtt aattcatcaa
    16741 caggagtgcc cacagtctcg tacatgtaga gagcctgcat tgcaaattca cgggcttttc
    16801 ttcgatgacc cattatcgta actgtttata caattgtgcc atttcaatag ctgccattgc
    16861 cgcatcaaaa cccttattgc ctgattttgt accagcacgc tcaactgcct gttcaatggt
    16921 atcggtggtt aaaacgccgt agataaccgg aatgtttgat tgcaatgaaa cctgcgccac
    16981 accttttgat acctcggcag caacatagtc aaagtgaggt gttgcaccgc ggataacagc
    17041 acccaggcat accactgcat cataattgcc ctttgacagc ttcattgcaa tgggtggtat
    17101 ttcaaatgcg ccgggcgtcc agaccacgca gatatttttt tcatcagcat tatgacgctg
    17161 taagcaatct aaagccccgg ataaaagttt gctggttata aattcattaa aacgcgaaac
    17221 aataatagct attgttaaac cggatgcgtc taattttcct tctattgtgt tcatacaaaa
    17281 cctgctcttc aaagatttta ttatgtgcga tagttcctga tatcagcatt gccatcgtta
    17341 tcgttaatag tagaaagtgt gtgatatcat gctcatttaa gtcaagttat tttttaacct
    17401 ggttactgtc atcttttcat cagcactgta taacgatact ttatgtttac actgtgcaat
    17461 agtatgacag caagcaatcc atgtaacagt aactatcgcc caccacacct gcgatagctt
    17521 atttactcat tgttgcctga acaaactcca atgttttacg aacatcatct ttgaaaaagc
    17581 actgcaaccc ctgggtccag cttccgtcgg taaggtcttt gcgaatacgg tcggcagttt
    17641 cctgacgcac gcataacttt aacggtgtaa acgcattgcc aggattattt ttccataggt
    17701 ttataagctc ttttgcccgg ctgataagct tatcctcgtc agccagctcg tcaaccagcc
    17761 ccatttttaa tgcttcgtca gggctaaaca tgttgccaaa gtacatgatg tccctgaatt
    17821 ttttgtcact gtcaagccca aaacgcacca cattccactg ggcaactgat agcggaagcc
    17881 ctatctttat ctcgctcatc cccattttaa tctttggatg attcttcatg atacggtaat
    17941 cacaagccat ggcaaaaatc ataccgccgg caacggtatg accgttgagt gcggcaataa
    18001 caggcttttc aaacataaaa agctctaata aaatttcttc ttcaacatca aaaaacttga
    18061 ttacctcgtt tatagtggta aagcttaaaa acatgggaag gtgaaaacca gatgaaaagg
    18121 tttttccctc gcctgtgagg ataacacctt taatggttgc gtcagatgct gcttctttta
    18181 aatactttct caggtcattc agtactgtat cggtaagcgc atttgtcttg ccatcattaa
    18241 gcgtcaggat gtacacatta tcttcaacct tttttgtaat catacaaagc cccctatagg
    18301 taatgattaa tcataattac atatcagtaa cacaatagaa acagttgccg tacatgtaaa
    18361 gaataaattt taaaacatgc cataattaac tacattgttg ttttattatt ttaccatatt
    18421 gtaccattgg gttatctgat aatagcatga tgcaccacac cgtcattcaa ggattaatcc
    18481 ggaattcaaa tgtgcaatgc tatatcctga atcagcgctc aggacatgat tcaggatgac
    18541 aggcggtact accccccttg tgcccccttt ataaaagctg gaaattgtgt gggtgagttc
    18601 attggcacaa aataggtttt attcttcaca attgttttta caatataatt tcgtagtaac
    18661 cacattatgc ttacttgata agttctgcat tcttcatggt aaaatttctt caaattgtgt
    18721 aaggatggaa ttgatactta ataattgggg tcagagccct gttaacttca ccctttcgtt
    18781 caaaggggag tagggggagt
//
LOCUS       DAOOJH010000042        18756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2912_length_18756_cov_9.530988, whole genome shotgun sequence.
ACCESSION   DAOOJH010000042 DAOOJH010000000
VERSION     DAOOJH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2912_length_18756_cov_9.530988"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLJ75_07105"
     CDS             complement(<1..1000)
                     /locus_tag="PLJ75_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05022.1"
                     /translation="MQKIFRLLSLLIVASIVITSSGCATVYRQQKKKINENYQEGIQL
                     YKQGDYKNAKEHFETVLSIDPQHSGAKQYLIITNEALQKRTKKYYNAGIQYKRKGDLE
                     NALIQFLQAQQRDPDYKDVKQQISNIRSSKYATKKYNTYYSTAKKQYEKKRYIAAYKN
                     CNKAELFDPNSLDLITLKARIKNQLDNNSYPYTSKAEAAKTKNPALAKKYCNKALAVN
                     PWDEKAQAIAKDIKRIEKLNDLYANGEKAYKKGDYVTAYRAFKQIDNNEPGYRNTANY
                     LATIKTKLEANINTYYQNGVTYYEQDNFKAAIAEWDIVLLINPDHQKAREYRERAVT"
     gene            1304..2266
                     /locus_tag="PLJ75_07110"
     CDS             1304..2266
                     /locus_tag="PLJ75_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD05023.1"
                     /translation="MEHKIIVSAKDIVKTFVMKKGALGKHGLVKAVDRVSLSIPEGNT
                     YGLVGESGCGKTTLARALLRLIEPDSGEIMIRNTPIHTLQKKELRQFRKNMQIVFQDP
                     YGSLNPRMTVEKIIAEALKIHCQLTKKDIVSRISELLAMVGLDKHFASRYPHELSGGQ
                     RQRIGIARAIALSPSFIVLDEPISALDVSIQGQILNLLLDIQEQCGMSYLFVAHNLAV
                     VKHISTMVGVMYLGKLVEENTASMLYRKPLHPYTRSLLSSIPDVKPVKHLFSVVKGEI
                     PSPENPPAGCHFHPRCPWVMPVCKEAYPAIVTVNGAKVACFLYH"
     gene            2248..3099
                     /locus_tag="PLJ75_07115"
     CDS             2248..3099
                     /locus_tag="PLJ75_07115"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05024.1"
                     /translation="MLSVSLTIGLLLVAVFNNSPAQDELAVTIEQKPQYTIYEREGIL
                     YCTGNTEAAVCNNKGADELAANNINTAIAIFQEGLVHAPLFYPFLYNIGIAYMRNRQF
                     DEARVYLEKAKHIVPENPRIYILIGELYAFTGREGDALVLFKQALNIHKKELDAIIKI
                     GTIYIERNQLELALRYFNSALDINPKYPDAGIGVAKVFFKKGEYYKALIRLKSIDITQ
                     GNYDKAYHYYYAESSYKLKDYKTAYEQYKQLLVYKDDIFFLTHSYALIEHRMNLARQF
                     AELENVQ"
     gene            complement(3136..6219)
                     /locus_tag="PLJ75_07120"
     CDS             complement(3136..6219)
                     /locus_tag="PLJ75_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05025.1"
                     /translation="MERDSLQSIKLELTKSLSFTHYVRICLHPVIASSHSQIAYALLC
                     SELQAEPAIRLSCIYTIVHYNIKGFTQHFHALLGQSITDEEIIAICSYLKVFGNVDST
                     AVLNNYVMHHYADESHHNAVIECLDTIRIVHQQDITLLTTLKTILNDTSAHHIIRYYA
                     LRALALYHDIKVLQSFLNQDESTLTGILDAIAFIGQDIINTNKEKDSTPGDSNLVIEL
                     RVFLSKLLPQFSEYSLKTKIACIHALIMCKHRETNEYLMKILTSDNESEKEHLLILLQ
                     HAITQLRDPEPFIRILISLGVVTPHHNKIIIENVLTFFTALQTDRTSTILKDKMFNYF
                     TVSLDSFFELYRKNYMISEVEEKNLPEIVQKIRNFIVSELTPQLLNMIVHYLKYNNNS
                     EIATILTPITNHIQQIDSSSREGFLLLIEMLYDSDPKSREISASRLETIDFEKRFLRD
                     RIVRLCAIIAKLNIQSAAPLLVKIYNYCKKYRDDVIFDACINTLAAMQYPYMLGELEL
                     MLLSGDAKEQLFALNYFIHYKDEHASAILFEVLKNTSTGNEVVQIALNLLTTMQIAQF
                     KGSTEILKNIVQTHASNSVKKAAILCIGHCGEGKDIEWLIAQFNESNDTAIKESILQA
                     ISSIIPRLRDFNKRPIIHFLLDCLKESGIRVRMYACAVLVQLNNKDVENHIKEMLIIK
                     NKEIQSEILFIFQNSKLPEFSFFLLSLLKEEYAIVSEIIHFLHNIPSEISDDIATFIG
                     NLYKKNGVDLSSVTPEAITIKHGKIEWLKKYYIMKITIPQESNPETLEETVSKRDTLH
                     SLIINHCKNNAVHIKYILPHSIVAYSENAMNIANACIAMEYDIKHFNQHAFNPFNAII
                     QCYHALVMEFGQYFIVLTMDRYLHNTLYNYGIIDDGLYTSIQNDYACNSLPYILASPA
                     YIPIYYIANKKNAPFEAQKALDEIIVMEKTKKEKEQQLSEEIRKRKLHIQSQGTAEYI
                     ETLEKVNAILRSEINEINKYVQKRSADRELNTQLLKMLENMQKKIFLEISSFMMK"
     gene            6491..6931
                     /locus_tag="PLJ75_07125"
     CDS             6491..6931
                     /locus_tag="PLJ75_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05026.1"
                     /translation="MKINDDLQAMIQSVAGDLGYMIYDVVLIIHGRSPRITVKIDHLQ
                     GISHYDCERFSKELSHRIDSRKLLDDYILEISSPGLKRELRTIDDYIRFTGAPVKGVY
                     LEDDVKKTFKGHLERVDNAIVTIDEGQKIIVVPYMQIVKANLDY"
     gene            6943..8265
                     /gene="nusA"
                     /locus_tag="PLJ75_07130"
     CDS             6943..8265
                     /gene="nusA"
                     /locus_tag="PLJ75_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPD05027.1"
                     /translation="MSGNFFEALHQIATEKGITREMIEEIVQSAMVSAYKKQYGITRP
                     VDIAFDRDKNTVKIISRKLVVRLPKNYAEEISLEDAQKIKPDAKFGDEVVVEENPLET
                     FGRIAAQTAKQVILQKIKEAEKNIVYDEYKDKVNELINGYVQRKTKEALYIDLGKTEG
                     IIPAREQSPLEHYKIGDRVKAIVLTVQKNSKGPSVVLSRAHPKFVEKLFEMEIPEIYD
                     DIVKIVSIVREAGLRTKVAVTSDRDDIDSVGACVGMKGVRIQSIVRELEGEKVDVIEY
                     SPNKKVMATNALTPAKVKEIIETRDGSVIAVVDNDQYRLAIGKNGHNARLAVKLCGFN
                     IDIKTEDQYREFLSSSESRALVEQLFATTQDGETPLEELPGLDARTITLLETGGIFSV
                     EDLVEKSVEDLMNIDGIGEKTAQKIINIIQEYVDFEEDDYEDEQEDKE"
     gene            8307..10787
                     /gene="infB"
                     /locus_tag="PLJ75_07135"
     CDS             8307..10787
                     /gene="infB"
                     /locus_tag="PLJ75_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPD05028.1"
                     /translation="MKAIDVATKNHVSTDDVIKICKELGIPCDNEQSDLHNDDVFLIE
                     KKIQIIKEHRAQEAKKLIQQAELKKKIKLKRKVHVAKELKKETDGKGETVIHEGTDAR
                     KKAEVKTAEPSKQGRDEHKDGQRKNEKFERKDRQGKPFNKFYKKDGKKDDQKLKHGGL
                     EQKPEKPSAQPLHDKKDVAVIDKEVVDLQKRQKDKGKEKEKEKDKDKRLLKKEKAEFD
                     EKLSLLKRKKEALPREAVTPREIEITETITVGDLAKKMNIKASEVIAKLMSMGVMATI
                     NQVIDADTASILAGEYGTEVKVVSLYEETVIRAEEEDRPEDYVLRPPIVTVMGHVDHG
                     KTKLLDAIRTTNVVDTEHGGITQHIGAYRVKVGEKYVTFLDTPGHEAFTTMRARGASV
                     TDIVVLVVAADDGVMPQTIEAINHAKAANVPIIVAINKIDLPEANPGRVKQELTTYEL
                     IPEEWGGTTLFAEISAKQKININELLDLILIQAEMLELKANPALKAKGVVIESKLDPG
                     RGPIATVLVQNGTLRVGDPFVVGLYSGRVRAMFADTGEQVYEATPSMPVEVLGISGVP
                     SAGDPFQVVESEKYARQIAQKRQELVRLESAQKVKKVTLEDLNAMIKEGEIKELKIVI
                     KADVDGSVQALRDSLQKLSTNDIRVKVIHAATGGINESDVMLASASNAIIIGYQVRPS
                     SKVAEIADKENVSIQYYNIIYDAIADVKAAMEGMLSPEMKEEITSTGEIKQTFKISKV
                     GTIAGAVVLTGRLKRSDKIRLLRDSVVVFDGKLNSLKRFKNDAGEVEAGQECGFTLEN
                     FNDIKEGDTFESYQIIEIKKKLGDNSRK"
     gene            10798..11178
                     /gene="rbfA"
                     /locus_tag="PLJ75_07140"
     CDS             10798..11178
                     /gene="rbfA"
                     /locus_tag="PLJ75_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPD05029.1"
                     /translation="MGYRKQKLEAQIQKLIGTLIVTELKDPRIGFVTVTRVKMSKDYA
                     YADVYISILGDEHDRQKTLEGLQSARGFVQYRVGKSLRIHTIPEVRFYVDTSIEEGVT
                     MVNLLEKLQKESAKNDNGDSSSKS"
     gene            11175..12119
                     /gene="truB"
                     /locus_tag="PLJ75_07145"
     CDS             11175..12119
                     /gene="truB"
                     /locus_tag="PLJ75_07145"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPD05030.1"
                     /translation="MKVSYSKGCVLVIDKPAGYTSFDIISIIKKKLKVKKAGHTGTLD
                     KFASGVLVLCTGWTTKLVPYFMDMDKRYIATIQLGIATDTHDCEGAIIATHEVNCTFE
                     EIDSCLQDTFVGTISQTPPLYSAVKIGGRRASDRVRRGEEITIAPRTVCIYAINVLNF
                     DKQRAQITLDVLCSKGTYIRALARDLGNSLQVGAHVVRLQRIAVGHFSVDNALRLEQC
                     DELPGKEIKNIDGVYAPYDALYEFGVVEVKEKALINIKHGKHLTADDVVTATEKNELF
                     RVIPEGQKVLVAIAHKQGEVYRCERVFVEEIDMLDTIL"
     gene            12260..12517
                     /gene="rpsO"
                     /locus_tag="PLJ75_07150"
     CDS             12260..12517
                     /gene="rpsO"
                     /locus_tag="PLJ75_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPD05031.1"
                     /translation="MVEKNEIINKYRVHEKDTGSTEVQIALLTERINHLTEHFKVHIK
                     DHHSRRGLLKLVGTRRRLLDYLKKKDVMKYRELIKSLGIRR"
     gene            12562..14649
                     /locus_tag="PLJ75_07155"
     CDS             12562..14649
                     /locus_tag="PLJ75_07155"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010491236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPD05032.1"
                     /translation="MNKEFAVTIGNDLIQFSTGILAKQADGAVVVSCGETIVFSSAVA
                     SKKVVEGQDFFPLTVDYREKHYSAGKIPGGFIKREGRPSDKEILTSRLADRPIRPLFP
                     DDFVNEVQIIIYVLSADREHQPDILAINAASAALSISGVPFKGPVGAVRVGRVDGQWI
                     VNPSFQEMENSDIDLVVAGTRKAVTMIEGQAKNLSEDAMIQAVEFAHANIIKLCEIQE
                     ELKNTCGKPLMQYIPKLSDNKLEKVIREKYFTAVENFKEYTEKKAREDAKDAIITAIT
                     NDLKEQFPDTIALVPEIVDAMDAEVIRSRILDEGSRPDGRGLNNIRPIDIMVGILPRA
                     HGSALFTRGQTQSLGIVTLGTVAESQRIDSIDLLEESYKRFMLHYYFPPFSTGEVGRV
                     GGVGRREIGHGMLAERAIEYAIPDEKEFPYTIRVVSEVLESNGSSSMATVCAGSLALF
                     NAGVPMKDQVAGIAMGLVLEGERYAILSDIQGIEDHLGDMDFKVAGTATGITAFQMDI
                     KIEGITTEIMRKALEQAKEGRLYILEKMNSVVSQPAQELSPHAPKIAVMKIEIEKIGE
                     VIGPGGRVIKKIIEETGAEINIEQDGTIFISANEQSAIDKAQQAIKGLIEEVEVGTIY
                     KGVVKRITDFGAFVEVLPGKQGLVHISKLDFNRVEKVKDVLKEGDEVMVKVIGIDDQG
                     RINLSRKDAMKKH"
     gene            14658..15920
                     /locus_tag="PLJ75_07160"
     CDS             14658..15920
                     /locus_tag="PLJ75_07160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPD05033.1"
                     /translation="MVHKFTLPDGLVVLLEPIDNVVSISCGLWIRVGSRHEKANEMGY
                     AHFIEHMLFKGTKKYSSKDIARLVDRVGGQHNAATNREYTCYYINVVSDYLELACEIL
                     SDTYFNSIFDAEEIEKEKNVVLEEIRMYEDTPDEHIHDLFMENMFYQSPLGHPILGVV
                     DTVTTINRDSIVEFYKQHYSDDNCLFAISGNFTVDNARTIIEKYFCSNCSPENNAPAV
                     MPQPSKRLYRYHMDKDLEQIHLCIGTDGLSKVDDDRWAYYLFNTILGGSMSSRLFQNI
                     REKEGLCYSIYCFHSSFMDTGIFGIYCGTSPSQYNKLLPLIANECKQIVSQGITQEEL
                     SDAKSYLIGNMALSLESTEVRMSHIAKNEMIYGRHFTFQEIAREIQTVTMDDFMRIAF
                     RIFNNAQFSLVTIGKLPQHHIDTFHLDL"
     gene            15981..17240
                     /locus_tag="PLJ75_07165"
     CDS             15981..17240
                     /locus_tag="PLJ75_07165"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05034.1"
                     /translation="MSENLQHIYRSLADILPQAHERKIVLITGARQTGKTTLAKKRYA
                     QLKYINLDSPEMRDSIRKVAASDWDKDVGIAVLDEAQKEPTIFEKIKYSYDEMSLPFS
                     VILGSSQILLLKNIRESLAGRIWLYELWPLMMSEMAAGSRRAEKPLLGAILEADSIDT
                     VLTAKPSILIGNENTIKKKAEDYLLQWGGMPALIHLESDNEKHQWLKNYNYTYLERDM
                     IDLSRLYDLEPFQKFQRLSAFRFSSLLNYSELARDAGISTDTARRYLEYLKISYQVLL
                     LQPYSINLTSSMVKTPKIYWIDMGIVRQLTGYYTNIPTGQLYENMVIAEMYKYIKTFH
                     SNANLYFYRTRSGLEVDIVIETPKGLMLAEIKNREDVVTGDYTAIREVANKMGTRFIG
                     GMVIYRGDKIYTLAQSIWAIPSHRLFT"
     gene            17280..17816
                     /locus_tag="PLJ75_07170"
     CDS             17280..17816
                     /locus_tag="PLJ75_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPD05035.1"
                     /translation="MLDAIITSKTRLKILLKFFINPSTKGYLRELSAQMNESTNAVRV
                     ELDRLVEAGYLERNANGNKVYYNANTMHPLYKDIHSVVKKYLGIDVLIDSVLAKLGKV
                     HEAYIVGDYSRGVDSGIIDVVIIGTVDYTYLAQLISKAEAIIKRKIRPLVLSPKEKKK
                     YDQLLHFAQALLVWHNGR"
     gene            17806..18306
                     /locus_tag="PLJ75_07175"
     CDS             17806..18306
                     /locus_tag="PLJ75_07175"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPD05036.1"
                     /translation="MEGNWYAVYVRSRHEFIVQTALAHKGLQTFLPAVERLRQWKDRK
                     KKVLFPLFPGYLFVQCNGDAAHFLAVLKTKGVVKILGDSSGYQPVPLQQIESLKTMIA
                     GRCAVDEYPYIKEGQKVRIAQGPLAGVCGILVKKGSEHKVVVSIDLLQKGVAVTVDGA
                     SIEPLV"
BASE COUNT         5888 a         3130 c         4205 g         5533 t
ORIGIN      
        1 ttgttactgc ccgttcacga tattcacgtg ctttttggtg atcagggtta ataaggagta
       61 ctatatccca ttcggcaata gcagccttaa agttatcctg ctcatagtag gtaacgccat
      121 tttgatagta ggtgtttatg tttgcctcta atttggtttt aatggttgca agataatttg
      181 cggtattcct gtatcccggt tcattgttgt caatttgttt aaatgcccgg taggctgtta
      241 cataatctcc ttttttgtac gctttttcac catttgcata gagatcattg agcttttcaa
      301 tccgtttaat atcttttgct atagcttgag ctttttcatc ccacggattg acagcaagcg
      361 ctttattaca atattttttt gcaagagcag gattttttgt cttagctgct tctgcttttg
      421 atgtgtaagg atatgaattg ttatcaagtt ggttttttat ccttgccttt aatgttataa
      481 ggtcaagact attgggatca aatagctcgg ctttattaca gtttttataa gcagcgatat
      541 atctcttttt ttcatattgt ttctttgcag tagagtaata agtattatat ttttttgttg
      601 catactttga actgcgtata tttgaaattt gttgtttaac atctttataa tcaggatcgc
      661 gctgctgggc ttgcaaaaat tgaatcaacg cattttctaa atcaccttta cgtttatatt
      721 gtataccagc attataatat tttttagtcc gtttttgcag ggcttcgttt gtaatgatca
      781 aatattgctt tgcccctgaa tgctgaggat caatagaaag caccgtttca aaatgttctt
      841 ttgcattttt atagtcacct tgcttataga gctggatacc ttcctgatag ttttcattta
      901 tcttcttctt ttgctgcctg tataccgttg cgcatcctga actggttatg acaatacttg
      961 caactatcaa taatgaaagt aatcggaata ttttttgcat accagcaccc catgactgta
     1021 cttatttttt atagatatat actaaaatta tatatgctat atatgcaagt aaaaattttc
     1081 acaataaatt agggttgaca attgattatt ttgcataact attgtggatt gctgcaatga
     1141 agcaatgtaa atgaataaaa atttttggcg atagttatcc tgaactctga ttcatgattt
     1201 gatgttaagc aatcgatgcg gagatgatcc tgttctgaag gcagactatg attcaacatg
     1261 gcaggaacgc ataataacag tgtagaaaag aatagcataa tcaatggaac ataaaattat
     1321 tgtaagtgca aaggatatag ttaaaacctt tgtaatgaaa aaaggagcgt tgggcaagca
     1381 tgggcttgta aaagctgttg atagggtaag cttatccata cctgaaggga atacctatgg
     1441 gctggttggc gaaagtggat gcggtaaaac aacattagcc cgtgcattac tccgcctcat
     1501 tgaacctgat agcggagaaa taatgatacg gaatactcct atccatacat tacaaaaaaa
     1561 agaattacgg cagtttagaa agaatatgca gatagttttt caggatccct atggttcgct
     1621 gaatcctcgt atgactgttg aaaagataat tgctgaagcg ctgaagattc attgccaatt
     1681 gacaaaaaaa gatattgtca gcaggatatc tgaattattg gcaatggttg gacttgataa
     1741 acactttgca tcacgctatc ctcatgagct cagcggcgga cagcggcagc ggatagggat
     1801 tgcgcgggcg attgcattaa gcccatcatt tattgtactg gatgaaccaa tatcagcgct
     1861 tgatgtatca atacagggac agattctgaa tttattgctt gatattcagg aacaatgcgg
     1921 gatgtcgtat ctttttgttg cacacaattt agcggttgtt aaacatatca gcactatggt
     1981 tggggtaatg tatctgggca aattggttga agaaaatact gccagcatgc tctatagaaa
     2041 gcctctccat ccgtatacgc gcagtttatt gtcgtccatc ccggatgtta aaccggtaaa
     2101 gcatttgttc tcggttgtga agggggagat cccctctccg gaaaatcctc cagcaggatg
     2161 tcacttccac ccacgctgtc cctgggttat gccggtatgt aaagaagcat atccggcgat
     2221 agttactgtt aatggagcaa aggttgcatg ctttctgtat cactgacaat agggttgttg
     2281 cttgtagctg ttttcaataa ttccccggca caggatgaat tggcagtaac tatcgaacaa
     2341 aaaccgcaat acaccattta tgagagggaa ggaattttat actgtacggg caacaccgaa
     2401 gctgcagttt gtaacaataa aggcgctgat gagcttgccg ctaataatat aaacactgca
     2461 atagcgatat ttcaggaagg gttagtccat gctcctttat tttatccgtt tctgtataat
     2521 attgggattg cctatatgcg caataggcag tttgatgaag cacgggttta tttagaaaaa
     2581 gcaaaacata ttgttccgga aaatcccagg atatatattc tgataggaga gctctatgct
     2641 tttaccggca gggaggggga tgctcttgtt ttgtttaaac aggcattaaa cattcataag
     2701 aaagagctgg atgccattat taagattggt actatctata ttgagcgcaa tcaactggag
     2761 ttagcattgc ggtattttaa cagcgcactg gatattaatc caaaataccc tgatgctggt
     2821 attggtgttg caaaagtttt ttttaaaaaa ggggaatatt ataaggcgct gatacgatta
     2881 aaatctattg atatcacaca aggaaactat gataaagcct atcactacta ttatgctgaa
     2941 agctcatata aattaaaaga ttataagaca gcatatgagc aatacaagca gctattagtg
     3001 tataaagatg atatattttt tcttacccat tcatatgctt taattgaaca caggatgaat
     3061 cttgccagac agtttgctga acttgaaaat gtacaatagc atactcaggc ttagcccaca
     3121 aaagtatttc catcattatt tcatcataaa actggaaatc tctaaaaata tttttttctg
     3181 catattttca agcattttta acaactgtgt gttaagctct ctatcggcgc ttcgtttttg
     3241 aacatatttg ttaatctcgt taatttcact tctgagtatt gcattaacct tttcaagtgt
     3301 ttcaatgtat tcggcagttc cctgcgattg tatatgaagc tttcgttttc gtatctcttc
     3361 gcttaactgt tgctcttttt ctttttttgt cttctccatg acaataattt catcaagggc
     3421 tttttgtgct tcaaatggtg cattcttttt attggcaatg taataaattg gtatatacgc
     3481 tggagatgca aggatgtagg gcaaactatt gcatgcataa tcattttgaa ttgatgtata
     3541 taaaccatca tctatgatgc catagttata taatgtatta tggagatacc tatccatggt
     3601 aaggactata aaatactgcc caaactccat aaccagagca tggtaacact gtataattgc
     3661 attaaatgga ttgaatgcat gctgattaaa atgcttaata tcatattcca ttgctataca
     3721 ggcattggca atattcattg cattctctga atatgcaaca attgaatgag gcagtatata
     3781 tttaatatgc acggcattgt ttttgcaatg attaataata agtgaatgca gggtgtcacg
     3841 tttgcttact gtttcttcaa gtgtttcagg attagattcc tgcgggatgg ttattttcat
     3901 gatataatat ttttttaacc attcaatttt accgtgttta atggtgatag cttctggagt
     3961 tactgatgac aagtccacgc catttttttt gtataaatta ccaatgaaag tggcaatatc
     4021 atcgcttatt tctgacggga tattgtgtaa aaaatgtatt atttctgata ctattgcata
     4081 ttcctctttt aaaagagaaa gcaaaaaaaa cgaaaattca ggaagtttac tattttgaaa
     4141 aataaataat atttctgatt ggatttcctt gtttttaatg ataagcatct ctttgatatg
     4201 gttttcaacg tctttattat taagctgaac caacaccgcg caggcataca tccgcacccg
     4261 tatccccgat tcttttaagc aatccagcaa aaaatgtatt attggccgtt tattaaaatc
     4321 tctgaggcgg ggtatgatac tgctgattgc ctgtagaatt gattctttta ttgcagtatc
     4381 atttgattca ttaaattgtg caataagcca ttctatatct tttccctctc cgcaatgccc
     4441 aatacaaaga atcgctgctt tttttacact attgctggca tgagtttgta ctatattctt
     4501 caatatttct gtgctgcctt tgaattgtgc tatttgcatg gttgttaaca gattgagggc
     4561 aatctgtaca acctcattac cagtggacgt attttttaaa acttcaaaaa ggattgccga
     4621 ggcatgctca tctttgtagt ggataaaata gttgagtgca aatagctgtt cttttgcatc
     4681 tccggataaa agcataagct ccagctcgcc cagcatgtag gggtattgca ttgctgccaa
     4741 tgtatttatg catgcatcaa agataacgtc atccctgtat tttttacaat aattataaat
     4801 tttcaccagc aaaggggcgg cagattgtat attgagtttt gcaatgatag cacatagcct
     4861 gactatccta tcacgcagaa atcgtttttc aaaatcaatg gtttccaatc gtgatgcgct
     4921 tatctctctg ctttttggat cgctatcgta aagcatttca ataagaagca aaaatccctc
     4981 tctgctggaa ctatcaattt gctggatgtg attggtgatg ggagtaagta ttgtggcaat
     5041 ttcactattg ttattatatt tcagataatg aacaatcata ttcaataatt gtggtgttaa
     5101 ttcggataca ataaaatttc ttattttttg aacaatttca gggagatttt tttcttctac
     5161 ttctgaaatc atgtaatttt tacggtatag ctcaaaaaac gaatccagtg aaacagtaaa
     5221 gtaattgaac attttgtctt ttaaaatggt acttgttctg tcggtttgaa gtgctgtaaa
     5281 gaatgttaaa acattttcta ttataatttt attatggtgc ggagtaacaa cccccagtga
     5341 tatgagtatc cgtatgaatg gttcaggatc ccgtaattgg gtaatagcat gttgcagaag
     5401 tatcagaaga tgttcttttt cactttcatt gtctgatgtg agtatcttca tgaggtattc
     5461 gttagtttcc cggtgcttgc acataataag tgcatgtatg catgctatct ttgttttcag
     5521 agaatattcg ctaaattgtg gtaaaagctt tgataaaaac actctgagtt caatgacaag
     5581 gttactatcg cctggagtag aatctttttc tttattggta tttataatgt cctgacctat
     5641 aaaagcaatg gcatccagta ttcctgtgag cgtactttca tcctgattta agaaggactg
     5701 caatacttta atatcatggt aaagtgcaag tgcacgtaat gcataatacc gtatgatatg
     5761 atgtgcagaa gtatcattta gaattgtttt cagtgttgta aggagtgtga tgtcctgctg
     5821 atgcactatt cggatagtgt ccagacattc tatgacagca ttgtgatgag attcatcagc
     5881 ataatggtgc atcacatagt tgttaaggac tgcagttgaa tcgacgttac caaacacttt
     5941 cagatatgaa caaatggcaa ttatctcttc atcggtaatg gattgtccta aaagagcatg
     6001 aaaatgctgc gtaaaacctt taatgttata atggactata gtataaatgc agcttaaccg
     6061 tattgccggt tcagcctgaa gctctgagca tagcagagcg tatgctatct ggctatgcga
     6121 tgatgcaata acaggatgca ggcatatcct tacatagtgc gtaaatgaca ggctttttgt
     6181 taattctaat ttaattgatt gtaaactatc acgttccata gcgcaccttt ttataaaggg
     6241 tttatacagt ttatataaca atagaaagta ttgtttgtaa tactgtaaat tagaatatta
     6301 cttttgataa aagtaaagat aaaaaaaagc ttgaaattgt aagcatatag taatatattg
     6361 attaattaag gtagttggtt ttctgctttt tgtttttctg ctttattgta tagtgtaatg
     6421 taaagcgggt ggcgtatgcc actctttttt ttaggctttt tatccttacc attattgttg
     6481 cgagataaca atgaaaatca atgatgattt gcaggcaatg atacaaagtg ttgccggtga
     6541 tctgggatat atgatatatg atgttgtgct cattatacat ggcaggtcac cgcgtatcac
     6601 tgtaaagatt gaccatctgc aaggaatatc gcattatgac tgtgaaaggt ttagcaagga
     6661 actatcgcac aggatagata gcagaaaact tctggatgat tatattcttg aaatatcatc
     6721 gccggggctg aaacgggagt tgcgaacgat tgatgattat atcaggttta caggtgcgcc
     6781 tgtgaaaggg gtatacctgg aggatgatgt aaagaaaaca ttcaaagggc atcttgagag
     6841 ggttgacaat gcgatagtta ctattgatga aggacaaaag attattgttg ttccctatat
     6901 gcagatagtt aaagcaaatt tagattatta aaggtgattg ctatgagtgg aaattttttt
     6961 gaagcgctac accagattgc aactgaaaaa ggtataactc gtgaaatgat tgaggagata
     7021 gtgcaatctg caatggtatc ggcttataag aaacaatatg gcattaccag gccagttgat
     7081 attgcctttg atagagacaa aaataccgtg aagataattt cacgtaaact ggttgtacgg
     7141 cttcccaaaa actatgctga agaaatatca ctggaggacg cacaaaagat taagccggat
     7201 gcaaagtttg gcgatgaagt tgtggtggaa gaaaatcctc ttgaaacgtt tggacgcatt
     7261 gctgctcaga ctgccaagca ggtgatatta caaaagataa aggaagcaga aaaaaatatt
     7321 gtatacgatg aatataaaga taaggtaaac gagcttatca acgggtatgt acagcgtaaa
     7381 accaaagaag cgctttatat tgatttaggg aaaacagagg gtattattcc tgcacgtgag
     7441 caatcgcccc ttgagcacta taagattgga gacagggtta aggcgatagt ccttactgtg
     7501 caaaaaaaca gtaaaggccc aagtgttgtt ttatcgcgtg ctcacccaaa atttgtggaa
     7561 aaactttttg aaatggagat acccgaaatt tacgatgaca tagttaagat tgtttccatt
     7621 gtacgcgaag ccggcttgcg taccaaagtt gcagtcacca gtgacaggga tgatattgac
     7681 agcgttggtg catgtgtggg tatgaaggga gtcaggatac agtctattgt gcgcgaactt
     7741 gaaggtgaaa aggtagatgt tatagaatat tctcccaata aaaaagttat ggcaacaaat
     7801 gcgctgacac cggcaaaagt aaaggaaatt atagaaacac gtgatggttc tgttatagcg
     7861 gttgtagata atgatcaata caggctggca ataggtaaaa atggacataa tgcgcgttta
     7921 gcggtaaaat tgtgtggatt taatatcgat atcaagaccg aggatcagta ccgtgaattc
     7981 ttaagttcaa gtgaatcacg ggcattggtt gagcaacttt ttgccacaac acaggatggt
     8041 gaaacaccat tagaggaatt gccagggctt gatgctcgta ctatcacatt gcttgaaaca
     8101 ggcggtatct tctcggttga ggatctggtt gagaaatccg ttgaggacct tatgaacatt
     8161 gatggtatag gagaaaaaac agctcagaag attattaata taattcaaga atatgttgac
     8221 tttgaagaag atgattatga agatgaacag gaagataagg aatagatgct cctgtcaata
     8281 attttagtat gaatagggat ttgttaatga aagcaattga tgttgcaaca aaaaatcatg
     8341 tttcaacgga tgatgttata aaaatctgca aggaattagg catcccttgc gacaatgaac
     8401 aaagcgattt gcacaacgat gatgtatttc ttattgaaaa gaagattcag attattaagg
     8461 agcatcgggc tcaggaagcc aaaaagctga ttcaacaggc tgagttaaaa aagaagatta
     8521 aattaaaacg gaaggtacat gttgcaaaag aactaaagaa agaaacagac ggtaaaggag
     8581 aaacggtaat ccatgaggga actgatgcca gaaaaaaagc agaggtaaaa actgctgagc
     8641 cgtctaaaca aggaagggat gaacataagg atgggcaaag aaaaaatgaa aaatttgaga
     8701 gaaaagacag gcagggtaag ccttttaaca aattttataa aaaagatgga aaaaaagacg
     8761 atcaaaaact aaaacatggc ggactagaac aaaagccgga aaaaccatca gcgcaaccct
     8821 tacatgataa aaaggatgtt gctgttattg ataaagaagt tgttgatttg cagaagcggc
     8881 aaaaagataa aggaaaagaa aaggaaaaag aaaaagataa agataaacgt cttctgaaaa
     8941 aggagaaagc agagtttgac gaaaaactgt cattattgaa aaggaaaaag gaagctttgc
     9001 caagggaagc tgttactcca agagagatag aaattacaga aacgattacc gtgggtgacc
     9061 ttgccaaaaa gatgaatata aaggcaagcg aggttatagc caagcttatg tcgatgggtg
     9121 tgatggcaac tatcaatcag gtcattgatg ccgatactgc ttcaatttta gctggtgaat
     9181 atggcaccga ggttaaggtt gtttcgctgt atgaagaaac tgttatacga gcagaggagg
     9241 aagataggcc agaagattat gttttgcgtc cacccattgt tactgttatg ggtcatgttg
     9301 accatggtaa aacaaaactt cttgatgcaa tcaggacaac caatgttgtg gatactgaac
     9361 atggtggtat tactcagcac attggtgctt atcgggtaaa ggttggcgaa aaatatgtta
     9421 cgtttttaga tacacccggc catgaggcgt ttaccacaat gcgtgcacgt ggtgcaagcg
     9481 ttaccgatat agttgtttta gttgttgctg ctgatgatgg tgttatgccg caaacaatag
     9541 aagcaataaa ccacgcaaaa gcagcaaatg taccaatcat cgttgctata aataaaattg
     9601 atttacctga agcaaacccg ggtcgtgtta agcaggaatt aaccacctat gagctgatac
     9661 ctgaggagtg gggtggaaca acgctttttg ctgaaattag tgcaaagcaa aaaataaata
     9721 tcaatgagct tttagattta attttgattc aggctgagat gctggaatta aaggcaaatc
     9781 cagcattgaa ggcaaaaggg gtggttattg aatctaaact tgatccaggc agaggaccca
     9841 tcgctacggt gctggttcaa aatggtacgt taagggttgg cgacccgttt gtggttgggc
     9901 tttattcggg cagggtgcga gccatgtttg ctgatactgg cgaacaggtt tatgaagcca
     9961 ctccgtccat gcctgttgag gtgctgggaa taagcggtgt cccttcggct ggcgacccat
    10021 ttcaggtagt agaatcggaa aaatatgccc gacagatagc tcagaaaagg caggagcttg
    10081 tacgtctgga aagcgctcag aaggtaaaga aggttactct ggaagacctt aatgcaatga
    10141 ttaaagaagg tgagattaag gaactaaaga ttgttattaa ggctgatgtt gatggctcag
    10201 tacaggcatt gcgcgactct ttacagaaac tttctaccaa cgacatacgg gtaaaggtta
    10261 tacatgcggc aactggtggt atcaatgaat cggatgttat gctggcatcc gcttcaaatg
    10321 caattattat tggctatcag gtgcgtccat catcaaaagt agcagagata gctgacaagg
    10381 aaaatgtctc gatccaatat tacaatatta tctacgatgc cattgctgat gtgaaggctg
    10441 cgatggaggg tatgctatct cctgagatga aagaagagat aacatccacc ggtgagatta
    10501 aacaaacatt taagataagc aaggttggaa ctattgccgg cgctgttgta ctcaccgggc
    10561 gcttgaaacg aagcgataaa atacggctgt tacgagatag tgtagtggta tttgatggga
    10621 agctcaattc gctgaagcgc tttaaaaatg atgcaggtga ggttgaagca ggacaggaat
    10681 gcggttttac actggaaaat tttaacgaca tcaaagaagg tgataccttt gaatcatatc
    10741 aaataataga gataaaaaag aaattgggag acaactcacg taaatagtgg cataaccatg
    10801 gggtacagaa aacaaaagtt agaagcacaa atacagaaat taattggcac ccttattgta
    10861 accgaactca aagatcccag gattggtttt gtaacggtaa cacgggtaaa gatgagtaaa
    10921 gattatgcat atgctgatgt atatatttca atcctgggcg atgaacatga ccggcaaaag
    10981 acattagaag ggctacaatc tgcacgcgga tttgtacagt accgggtggg gaagtcatta
    11041 cgtatccata ctataccgga agtgaggttt tatgtggata cgtcaattga agaaggcgtt
    11101 accatggtaa atctgcttga gaagctgcaa aaagaatctg ctaaaaatga taatggtgat
    11161 agttccagta aatcatgaaa gtttcatata gtaagggttg cgtgctggtt atagacaaac
    11221 ctgcaggata cacctctttt gatattatca gcataatcaa aaaaaaattg aaggtaaaaa
    11281 aagctggcca tacaggaacg ctggataagt ttgcatcggg tgttctggtt ctgtgtacgg
    11341 ggtggactac taaactggtg ccgtatttta tggatatgga taagcggtat attgcaacca
    11401 ttcagttagg gattgcaacc gacacccatg attgtgaggg tgccatcatt gcaacccatg
    11461 aagtaaactg tacctttgaa gagatagatt catgtctgca ggatacattt gttggtacta
    11521 tcagccagac cccgccgctt tactcagcgg taaaaatcgg cggaagacgc gcttcagata
    11581 gagtacgacg gggtgaagag attactattg ccccgcgaac agtctgtatt tatgcaatca
    11641 atgttcttaa ctttgataag cagcgcgcac agattacact ggatgtttta tgcagcaagg
    11701 gaacctatat cagagcactt gctcgggatt taggtaacag tttacaggtg ggcgctcatg
    11761 ttgtgcggct gcagcgtata gcggttggac atttcagtgt tgataatgct ttacggctgg
    11821 agcagtgtga tgaactgcct ggcaaagaaa taaaaaacat cgacggggta tatgctccct
    11881 atgacgcctt atatgaattt ggtgtggttg aagttaaaga aaaagcgctt attaatatta
    11941 agcatggtaa acaccttact gctgatgatg ttgttactgc cactgagaaa aatgaactgt
    12001 tcagggtaat ccctgaagga caaaaggtgc ttgtggcgat agctcataaa caaggggaag
    12061 tgtaccgatg tgaaagagtt tttgttgaag aaattgatat gcttgataca atactataat
    12121 agtatatata aaagtatata taaatttatt tcttgactga ttttagcttg caatagcagt
    12181 gttaacagtt acgattagac taactatgaa taaaaagtat tttaagtaaa gtgataggta
    12241 aggaggagaa aagcgtcgta tggttgaaaa aaatgagatt atcaacaaat accgggtgca
    12301 tgaaaaggat accggttcaa ctgaggtaca gattgctctt ttaactgagc ggattaatca
    12361 tcttactgag cattttaagg tacacatcaa agatcatcat tcaagacgtg gattgcttaa
    12421 gctggtaggt acacgaagaa ggctgcttga ttatcttaag aaaaaggatg tgatgaagta
    12481 cagggaactg attaaatctc ttggtatacg aaggtagagc agaaacaatg gtattacatt
    12541 ttaggtgtga aggaatgaac aatgaataag gaatttgcag taacaatagg aaatgattta
    12601 attcagttca gtacaggcat tttagcaaag caggcagatg gtgcagtagt tgtttcctgt
    12661 ggtgaaacca ttgtattttc atctgctgtt gcttcaaaaa aggttgtaga agggcaggat
    12721 ttttttccat taacggttga ttaccgggaa aagcattatt cagcaggcaa aataccgggt
    12781 ggatttatta agcgtgaggg cagaccaagc gataaagaga tccttaccag ccggttggcc
    12841 gaccgtccca tacgtcctct tttcccggat gattttgtca acgaggtgca gattattatc
    12901 tatgttttat cagcggacag ggaacatcag cctgatatcc tggcaataaa tgctgcttcc
    12961 gcagcgttaa gcatctcagg tgtaccgttt aaaggaccgg ttggcgctgt aagggttgga
    13021 agggttgatg ggcaatggat tgtgaatcca tcttttcagg aaatggaaaa cagcgatatt
    13081 gaccttgttg tagccggtac cagaaaagcg gtaaccatga ttgaaggaca ggcaaaaaat
    13141 ttatctgaag atgccatgat acaggcagta gaatttgccc atgcaaatat cattaaactg
    13201 tgcgaaattc aggaagaatt gaaaaatacc tgcggcaaac cattaatgca gtatatacca
    13261 aaactgagcg ataacaagct tgaaaaggtt attcgtgaga agtactttac tgcagttgaa
    13321 aattttaaag aatatacgga aaagaaagca cgtgaagatg caaaagatgc tatcataacc
    13381 gctattacca atgacctgaa agaacaattt cccgatacaa tagcacttgt tcctgagatt
    13441 gttgatgcaa tggatgccga ggtgatacgc tcacgtatac ttgatgaagg ttcccgccct
    13501 gatggcagag gactgaacaa tattcgtccc attgatatta tggttggcat ccttccacgg
    13561 gctcatggtt cggcgctgtt tacccgtggt caaacacaaa gccttgggat agttaccctt
    13621 ggaactgttg ctgagtctca gcgtattgac agcatagatt tactggaaga atcatataag
    13681 cgcttcatgc tgcattacta cttcccgcca tttagcactg gtgaagttgg cagggttggc
    13741 ggagtaggaa gacgcgagat aggacatggc atgcttgccg aacgcgccat tgaatatgct
    13801 attcccgatg agaaggaatt tccgtataca atacgcgttg tttcagaagt tttagaatcg
    13861 aacggttcat catcaatggc aacggtatgt gccgggtcac ttgctttgtt taatgccggg
    13921 gtgccaatga aggatcaggt agccgggatt gccatgggat tagtactgga aggcgagcga
    13981 tatgcaatct taagtgatat tcaggggatt gaggaccatt taggtgatat ggattttaag
    14041 gtggcaggca cggcaacagg tattaccgca tttcagatgg atatcaagat cgaaggcata
    14101 actaccgaaa ttatgcgcaa agcgctggag caggcaaaag aaggaaggct ctatatactg
    14161 gaaaagatga attcagtcgt ttcccaacct gcacaggaac tatcgcccca tgcgccaaaa
    14221 attgccgtaa tgaaaattga gatagaaaag attggtgagg tcataggacc gggcggaaga
    14281 gtaataaaaa agattattga ggaaacgggt gccgagatta acattgaaca ggatggtact
    14341 atctttattt cagccaatga acaaagcgca attgataagg cacaacaggc aatcaagggg
    14401 ctgatagaag aggttgaggt tggcaccatc tataaaggtg ttgtaaaacg tattaccgac
    14461 tttggtgcat ttgttgaagt tttacctggt aagcagggtc tggtacatat ttcaaaactg
    14521 gattttaacc gtgttgaaaa agtcaaggac gtgcttaagg aaggcgatga ggtaatggtt
    14581 aaggtgatag gcattgatga tcagggcagg attaatctaa gccgtaaaga tgctatgaaa
    14641 aaacattaga ttgtcccatg gttcataaat ttacacttcc tgatgggctt gttgtcttgt
    14701 tagagccaat tgataatgtt gtatctattt cgtgcgggtt atggatacgg gttggttcgc
    14761 ggcacgaaaa agccaatgaa atggggtatg cccatttcat tgagcatatg ctgtttaaag
    14821 gcaccaaaaa atacagctca aaagatattg ctcgccttgt tgatagggtt ggcggccagc
    14881 ataatgcagc taccaatcgt gagtatacct gctattatat caatgtggtt tctgattatt
    14941 tagaactggc atgcgagata ttgtccgata cctactttaa ttccatcttt gatgccgaag
    15001 agatagaaaa agaaaaaaat gttgtccttg aagaaatacg catgtacgaa gatacccccg
    15061 atgaacatat acatgatctt tttatggaaa atatgtttta tcaaagtcct ctgggtcatc
    15121 ctatcctggg tgttgttgat acggttacta caattaatag agattccatt gttgagtttt
    15181 ataaacagca ctatagtgat gataattgcc tttttgccat ctccggcaat tttactgttg
    15241 ataatgcccg tactattata gaaaaatatt tttgcagtaa ttgttcacca gaaaataatg
    15301 ctccggctgt tatgccacag ccatcgaaac gattgtaccg ctaccatatg gacaaggatt
    15361 tagagcagat tcatctgtgc attgggacag atggattaag taaggttgat gatgacaggt
    15421 gggcatatta tctctttaat actattctgg gcggcagcat gtcatcacgc ctttttcaaa
    15481 atatacgtga aaaagagggt ttgtgctaca gcatatattg ttttcattcg tcatttatgg
    15541 acaccggcat ttttggaatc tattgcggga catccccgtc acaatataat aaattattac
    15601 cgctgattgc caatgagtgc aaacaaattg tatcacaggg tattacccag gaagagcttt
    15661 cggatgccaa aagctattta attggcaata tggcattgag cctggagagt actgaagtgc
    15721 gcatgagcca tatagcaaaa aatgagatga tctatgggcg acattttacg tttcaggaga
    15781 tagcgcgtga aatacaaacc gtaaccatgg atgactttat gcgtattgcc ttccgcatct
    15841 ttaacaatgc acagttttcg cttgtgacca ttggaaaact cccccagcat catatagaca
    15901 cctttcatct tgatttgtaa tgcgatagca actgtagttt tttcttgaca atcgtacatt
    15961 ttgctgtaaa atttacgatt atgagcgaaa atttacagca tatttaccga tcattggctg
    16021 acatactacc gcaagcacac gagcgtaaaa tagtgcttat taccggtgcg cgtcagaccg
    16081 gcaaaactac attagcaaaa aaacgatatg ctcaattgaa gtatattaat cttgattcac
    16141 ctgagatgag agatagtatc cggaaagttg cggcatcaga ttgggataag gatgtgggga
    16201 tagctgtcct tgatgaagct cagaaagagc ccactatttt tgaaaagata aaatacagct
    16261 acgatgaaat gtcattgccg ttctcagtta ttctgggatc aagccagata ctgttgttaa
    16321 agaatatcag ggaatcatta gcaggcagaa tctggcttta tgaactgtgg cctttaatga
    16381 tgtccgaaat ggcagcgggt agcagacggg cagaaaagcc attgctgggg gccattcttg
    16441 aagctgattc tatagacaca gtgttgacag caaagccatc aatattgatt ggcaatgaaa
    16501 atactataaa gaaaaaggct gaggattatt tattgcaatg gggggggatg cctgcgctta
    16561 ttcaccttga atcggataat gagaaacatc agtggctaaa gaattacaat tatacctatt
    16621 tagaaaggga tatgattgat ctatcacgtc tgtatgatct tgagccgttc caaaaatttc
    16681 agcgtctttc agcgtttaga ttttcatctt tgcttaatta ctctgaatta gcacgcgatg
    16741 ctggcattag cactgatact gcacgcagat atcttgaata cttaaaaata tcatatcagg
    16801 tgttattgct tcagccatat agtattaacc ttaccagctc gatggtgaag accccaaaga
    16861 tttattggat tgatatgggc atagtacgac agctaacggg atactacacc aatattccaa
    16921 ccggacaact atatgaaaac atggtaattg ctgaaatgta taaatatatt aagacattcc
    16981 atagtaatgc aaatctttat ttttatagaa ctcgttcggg attggaggtt gatattgtca
    17041 tagaaacgcc aaaaggattg atgcttgctg aaattaaaaa cagagaagat gtggtgacag
    17101 gtgattacac tgctatacgg gaagtggcaa acaagatggg cacacggttc attggaggaa
    17161 tggtcattta ccggggggat aaaatatata cattagctca atctatatgg gctataccat
    17221 cacatcgtct ttttacataa acgcttgaca aaattgagtc aaaatagtac tcattaacca
    17281 tgttagatgc tatcataaca tcaaaaacgc gccttaagat actgctcaag ttttttatca
    17341 atcccagcac aaaaggctac ttgcgggagc tatcggcaca gatgaatgag tccaccaatg
    17401 ctgtgcgcgt ggagctggac cgccttgttg aagcaggcta tttagagcgg aacgcaaacg
    17461 gcaacaaagt atattacaac gccaacacca tgcatccact gtataaggac atacacagcg
    17521 tggtaaaaaa atatttaggc attgatgtgc tcattgacag tgtgcttgcc aagctgggga
    17581 aggtgcatga ggcgtatatc gttggcgact attccagggg tgttgacagc ggcatcattg
    17641 atgttgtcat cataggaacg gttgattaca cctatttagc gcagttgatt agcaaagccg
    17701 aagctattat caaacggaag atacgtccgc tggtactatc gccaaaagaa aaaaagaagt
    17761 atgaccagtt actccacttt gcccaggcat tgctggtgtg gcacaatgga aggtaactgg
    17821 tatgccgttt atgtacgctc gcgtcatgag tttatcgtgc aaaccgcatt ggcgcacaaa
    17881 ggcttgcaga catttttgcc agctgtagag cgccttcgtc agtggaagga ccgcaaaaaa
    17941 aaggttttat tccccttatt tcccggatat ctgtttgtac agtgcaatgg tgatgctgcc
    18001 cattttttgg cggtgctgaa aacaaaggga gtggtaaaaa ttctgggcga tagctccgga
    18061 tatcagcctg ttcccctgca gcagattgaa agccttaaaa caatgattgc cggcagatgc
    18121 gcggtggatg agtaccctta tattaaggaa gggcagaagg tacggattgc tcaaggaccg
    18181 cttgccggag tttgcgggat actggtaaaa aaggggtcag agcataaggt ggtggtgagt
    18241 attgacctgc tgcagaaggg cgtggcggta acggtggatg gtgccagcat tgaaccgctt
    18301 gtgtgaagca caaaaaagta ttgactgctt tgttcttcgc aatggtatac agggattacc
    18361 acagtgttgc aaaaacataa taaaagtgct tgacaaataa tgagtaacta ttttactcat
    18421 tttatataag cagtgtcata ctgtatcgtt tttttattat tatacgcatg aagctatata
    18481 gagcgatagt tattggtaaa gctcagtata aaaaaaatgt ctgtaactga gagggcgaag
    18541 ctttgctgtc ataatgtatg gtatgaacat agagcgtata ccaatacgct tgacgaggga
    18601 gttaagcagt gctatggtta cagcagcaaa gcgcatacat ggtgtaaaaa aatgacagca
    18661 ctatagaagc ggtttatata cataaaaata ctttgacatg attatagaaa atagtatact
    18721 atagataaaa caggaggaaa tgtacccatg ggatat
//
LOCUS       DAOOJH010000043        18395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_2984_length_18395_cov_9.861396, whole genome shotgun sequence.
ACCESSION   DAOOJH010000043 DAOOJH010000000
VERSION     DAOOJH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18395
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2984_length_18395_cov_9.861396"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1125)
                     /locus_tag="PLJ75_07180"
     CDS             complement(115..1125)
                     /locus_tag="PLJ75_07180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD05037.1"
                     /translation="MRSWHLHNYIVGDISITLLSSEIIIIHRRKNNAFIKSISWRSFR
                     RAGIAFAAVCLCLVVLFILAGINDIFFSEASWYDEIKKKTIANKEFEEPRERSILKIV
                     HHTIKQGETLSEIAGMYGVSMDTICGCNKLESYDLVHVGQVLTIPNKDGLIVSVQSGQ
                     TLPSLARKYNVGIQKIIAENVIANPDFINVGQHIFIPDAKPLDIFKGFLWPVNNQYIT
                     SGYGWRKDPVYGDKQFHQGIDVRANYEWIHASKYGRISYAGWLGGYGKVIVVTHPGGD
                     RTLYGHLSRIIVKEGQYVKQGQIIAKSGNTGNSTGPHLHFEIIRKGQHINPYVELKKK
                     RK"
     gene            complement(1157..1582)
                     /locus_tag="PLJ75_07185"
     CDS             complement(1157..1582)
                     /locus_tag="PLJ75_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD05038.1"
                     /translation="MAGKSDSLNSTIGEGSVFEGKFYISGSLRIDGKFEGEIKTDEEL
                     VVGETGKVKTNIHAKNVVVAGTVIGNIVADEEVKLLETAKVLGDIETPILTVQRGVVS
                     QGVVTITGGQKKDVKKLIEESYAGTPQAAGIFSKKGSEK"
     gene            1779..2435
                     /locus_tag="PLJ75_07190"
     CDS             1779..2435
                     /locus_tag="PLJ75_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05039.1"
                     /translation="MNILQTPIVVNEIFYSLQGETTRTGFPSLFIRLAGCNLNCRWCD
                     TAYARTGYTMTIASIVKKIQQYPLANHITITGGEPLIQQGTIALLNILCTHNYPLQLE
                     TNGSISLEKVPQPVHRIVDVKTPSSGEEKSFNFTNLQLLQPHDEIKFVIANDNDYNYA
                     KEFMQKYCANTCCVINFSPVVLTMKPQTLAEYILRDRLPVRLNVQLHTMLWQQEPKHF
                     "
     gene            2445..3317
                     /locus_tag="PLJ75_07195"
     CDS             2445..3317
                     /locus_tag="PLJ75_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05040.1"
                     /translation="MDYVGHIIRPPSEAYSMIIQVTVGCSHNMCTFCGTYKDQKFYVK
                     DMQQVKRDIDEASRYRFDRVFLTDGDVLILPTETLLDLFAYIKKKNPHIERIGVYANT
                     KAILKKSVAELKQLKDAGLGIVYHGIESGNEEVLRRIRKGALPHKQLEAAQKIKEAGI
                     ALSQTVLLGIGGIDLSIPHAMDTGKHLGAMSPDYASALTVMLLPNTPLYRDYVQGKFV
                     LPDKFGLLRELKLMIENMDVTSTCYFTSNHASNYLPIKATLPDDKIQVISLLDHIINT
                     KDESILKPEYLRAL"
     gene            3332..3493
                     /locus_tag="PLJ75_07200"
     CDS             3332..3493
                     /locus_tag="PLJ75_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05041.1"
                     /translation="MDKSMEEAIRFISFELQESPGTDIAKLIEKASQQFDLTPMQTEF
                     LVNKFILNK"
     gene            complement(3729..4811)
                     /locus_tag="PLJ75_07205"
     CDS             complement(3729..4811)
                     /locus_tag="PLJ75_07205"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPD05042.1"
                     /translation="MIRGLVYLVIISLAIIGSCAKQKVDDYASIAFSIGDVKKNGQLA
                     VIGEAINQRDIIETGALSSCDIKIGDSIIRIKEKSKAIMAQFLRKDGIENTTLGLDIG
                     KMICKPKKLLKNESFLVKTPTAVAGVRGTNFSVEADAKKTTRIKVFEGKVAVVKRVEA
                     VEEHVDKIIEVAPVVEEKEKVIITADDVKKAEKKVEVIIAKEGESPKAIEKVIAVAKE
                     EIVVSRDKVEKFKPEDFNDEKQGIIKVEEKPKEVVKEVAKVVKKDKQIPKPEGQLLVT
                     RYEIYFVKNGKVEWEGKVVSPPTKADDKIYIASGDYIFCAKLDGTVLWRKKLANDGKI
                     ELEGEKLAVYAGGQKKILDKLTGEEE"
     gene            complement(5077..5826)
                     /locus_tag="PLJ75_07210"
     CDS             complement(5077..5826)
                     /locus_tag="PLJ75_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD05043.1"
                     /translation="MLRVEHLFAGYDSKIVLKDISLQLHRGTVAALLGPNGSGKSTLL
                     KAIDGIIKPDNGAIILDTMNINAMTKNNIAQSIAYLPQSGNSFPSCNVFESILLGRKP
                     YISFEPTKHDMEIVEKTINNFNIDSLAFRTINQLSGGEIQKVLIARAIVQEPEVLLLD
                     EPINHLDIKNQIEILQIVRYYTINYNLITLIVFHDINRALQFADKFIVLKEGYVYGDG
                     DSKIVNPEIMKGVYGIDLDCIEYKGRKLLFV"
     gene            complement(5826..6917)
                     /locus_tag="PLJ75_07215"
     CDS             complement(5826..6917)
                     /locus_tag="PLJ75_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015790968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPD05044.1"
                     /translation="MAKLNLPMRDSPYIKSGLMQQYLQLLYVKLGIGFVFLLCLGAAF
                     LFSLTAGSLHIPFGKIITTLFWQNDLSYVIYNIRLPRSVAAVIAGASLGVSGCVIQNI
                     LKNPLASPFTLGISHGAAFGASVAIIGLGAGITHSYGNESVSVFNVYMVVFAAFAGAI
                     VAVSIILLLSLVKKVSPEAIVLAGVAMSSLFGASTMALQYFATDFQVSATIFWTFGDI
                     GKAGWVENLIMTIGFIACFVYFYIHRWNYNALLWGDDVAKSLGVNSTSLRLLGMLLCA
                     LIVSLCTAFLGIIGFVGLIAPHIVRMIIGNDYRFLIPYSALFGALFLLISDSIAKMLL
                     SPVILPVGIITSFLGAPMFFYLLVRRSTL"
     gene            complement(6874..7896)
                     /locus_tag="PLJ75_07220"
     CDS             complement(6874..7896)
                     /locus_tag="PLJ75_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD05045.1"
                     /translation="MITDFAGRRVAIPEEIKGVVALSASLRYVVYLQAFDKVVGVEEL
                     EKRNSMRTINADGRMYWTAIADRVGAIPGIGEGGPGRLPDFERLMEVNPDIVFTFEAN
                     NAEIIQRKTGIPCIVISGATGMDGFSIDDIKKTFLFMGNILGKQVRALQLCTYIDTCI
                     ADLQQRVHNTKKVSVYVGGVSARGAHGITSTESFYPPLQWLNVLNVADELGKKGHLFI
                     DMEKLIVWNCDNLFIDAAGLALIEIDYKKNSGFYKKLKAVVKNNVYTVYPYNFYRTNI
                     EVMIANSYFIGKTLYPERFNDIDTEEKARVIVKEFVGMDVYDNLKKSYKGYGKVKFTN
                     AGLTVH"
     gene            complement(8019..8423)
                     /gene="tsaA"
                     /locus_tag="PLJ75_07225"
     CDS             complement(8019..8423)
                     /gene="tsaA"
                     /locus_tag="PLJ75_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPD05046.1"
                     /translation="MESISYPIKPIGYIRHRHKDVPRHWSLSELEGEIVIDEDYALGI
                     HDIKAGNLIVVLFLFDRAVSFTMDRLIVKPPHCDEYLGVFSTCSPVRPNPIGLSIVEV
                     LEVHGFTIKVKRIDMFDGTPVLDIKPYIPYMS"
     gene            complement(8439..8882)
                     /locus_tag="PLJ75_07230"
     CDS             complement(8439..8882)
                     /locus_tag="PLJ75_07230"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPD05047.1"
                     /translation="MKKIAICGKGGVGKSLMVYLISKALIDKNCHVLIVDADESNTGL
                     HRMFGFSKMPQSFMEYLGGKKVVQQTMLKRFRNGKNEPEMSIIEKEQFDTNNIDDDYI
                     VKEGPLSFIFIGKIYHPLEGCACPMGAVSTAAQEVRAIIGNIFTH"
     gene            complement(8910..11180)
                     /locus_tag="PLJ75_07235"
     CDS             complement(8910..11180)
                     /locus_tag="PLJ75_07235"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPD05048.1"
                     /translation="MDGFKVFSFIGIIICMGMTMVFAQTQTEADKTNDENNGMVILEE
                     IVVEGAKLIIPTKQAGETVYTGTEVTRKGMELKGEKGKANVYESISIVPGVVFEGIDP
                     GNMVSEQNSIRMRGVRGFMGAMTVDGIPNYGGNPMGPRAYVYDMENFDSIAVFKGAVP
                     ADLGVGVGDRGGAVELKPRWAQDTFGIALVSSYGSFDLMKMFVRVDSGKVGPADSRLS
                     VSYSCTKEDKWKGPGDLGPRHNANVTFVQPMGKYMTISLWGNYNTIHHYKYRSLSYEQ
                     AKDLDDYYKYDFNGGLTGIPGEDMFYYKFNEEEHTNCDFFTLINAHGKDFSVVIKPYI
                     TNEDATIRDGANIQNQPGVQKRTRDIVRKGVVSQISIDLSMIKATAGYHFEQSDMNIY
                     TQNYWIQNDGSLLYRGYGMYTTSGDTFINSPYGKLAGEIKNFKWQVGVKYFRFKESDS
                     RGYTTTYPGSVPALTRAPDLDRDERIYDIMLPTMGVSYSFTKYFEVYSSYGKNFIRPY
                     AYMPLQNLYNRLRTQFQAAGITLNDLFKGYDIEQSDNVDVGVRIKRHYLEINPTVFVS
                     QHKNLLVNVTDSRVTDSGTGKAVSYQQNAGKAKGYGCELGTSIFVSRWATIFVNPTYN
                     HLVYDGNSTYQGTVYDNDGRQIVDTPQWVVLAGIIGKYKQLELVPMLRYIGKRYGDIM
                     HHEQIDAYSVFDVKVSYCKDKIHMLKNVTFSLEFDNIFNKKYVSVINASDDTVSGTSY
                     YAGAPFSVKGSVSCMF"
     gene            11411..12634
                     /locus_tag="PLJ75_07240"
     CDS             11411..12634
                     /locus_tag="PLJ75_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05049.1"
                     /translation="MNDDWYNTAILQSIIIADAFASPLDNLSAQHIKATFDDVTEYVD
                     PEPALKHKLYLWKKPGLYTSLSQYCILLCAINSQNYTYNHQLVINFLHSLGDSSAYFR
                     HTPQILHNLGNAYDAPTAELLVLIPSLLFCKREPTPQNLLHFILAHNKNAATCAACTF
                     FYLLLKEIIGRQLSTFHPEILNDISSQMALLIQQHSASIFEHGANPQVITQAASDLHA
                     LMTTLPLTSDEVTFTRHILNYANKWSNNSFTRLTVNHPFAILPFALYCSYSTPYNAVF
                     SHIHHGGRVSVLLPLIAAISAALYGPDSIPEHLKETLINKKRIYQLLQFLHESSITMN
                     YLTEFFQNEMKLTQKEKDELESKLKHSKHIKEKPKKPAADKHNAMTNHVVESWTKLDK
                     AKWKKEKKKHKPYSE"
     gene            complement(12622..13515)
                     /locus_tag="PLJ75_07245"
     CDS             complement(12622..13515)
                     /locus_tag="PLJ75_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05050.1"
                     /translation="MRHNNCYYCGINGFIFKSYRFEDMAIIADIKIEKKHEGWPGIPH
                     GGLSMAAFVELIDIAKESAALYPHEYHFRFGGERLSCGDAVRCIVQKGDEKNFFGIFG
                     RSLQNPYCHCRYSETRVHPYNNDVIDVLKQNMDAATPFIIPNMSVNLINNKPGNNNSR
                     MFYITDYQLDRSYLIARLHAPNNFVPMDDSSIHQGVLCSLLDETMGWSTFLSVWQGGV
                     TVELKITFFDKVYNSDDVYVIGFCDTVKGEYGKKIASAYGGVFAQKDDDMEIIALAHG
                     RWLTLAGFKEIMLNYLKHYSE"
     gene            13985..14845
                     /locus_tag="PLJ75_07250"
     CDS             13985..14845
                     /locus_tag="PLJ75_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05051.1"
                     /translation="MVLILVTVLISTTGIAIDFSFQPIVIENLSRYNEDRIAYQHVQK
                     DVSPNLDNSFSALLIIKSKKVYLIQDGYDNPENVSTKKLQFEMENKPLDDPWENKINN
                     KPDYVRITERKIELLKNFGEDFVSKNFGNFFLSVRNALIKKHVEVFKTLMVDRKESGL
                     VVTRTPLPVPAYLNAPETPTKYKMTVSGKTIDEKLYYAEDIDGDGITETFTVNSPDGF
                     NWGYKSGPNIILILNNKDEEIKGLIGQLCNWAYYGTPEEEKEILHNFPTDMDIINEFK
                     LEAVPTQQTK"
     gene            complement(14892..15791)
                     /locus_tag="PLJ75_07255"
     CDS             complement(14892..15791)
                     /locus_tag="PLJ75_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD05052.1"
                     /translation="MTYRYIDLHIHSQYSEDGDYSVDHIFQMAKTIGLSAISITDHDA
                     IDAIPEGIVCSEKYNIEFIPGIELTTIYEDGSQQHILGYFINNAPVLNAIVKKIGNYR
                     HEIAKKRIQKLRDIGFVMNEKNVWRKANNRPPTATAIMLEVFENPHNRNDKRLEEYFN
                     GKKSDNKLMYFYREYLAEHMPAYVPFISISTHEGIEVVKATGGVPVLAHPKFVKGEEH
                     LKKIAVMGIAGIEAISSYHTKEDIDYFIRFAADHNLIITAGSDFHGPTSKPKVTLGGI
                     QGNDYRLLDELKKYRERSKTVIQ"
     gene            complement(15811..16533)
                     /locus_tag="PLJ75_07260"
     CDS             complement(15811..16533)
                     /locus_tag="PLJ75_07260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD05053.1"
                     /translation="MNIQKCVIVFLLVIVSFSFAGELYFIDNENIQRYNGKSGKWIEV
                     NMVKQIKALANQFGITVEDVYCINNIKEKQFKRSFVFFPYSDIYIQQLMEKGISLQPL
                     NVPYDAFIWPIGDPEKISSGLGRRWGRFHPGVDIPAPKGTMVRATMEGKVIYAGYFGN
                     YGNSIVLEHRDNYVTRYAHNSVLLVKVGDYVQKGQAIAFVGSTGRSTGNHLHFEIRCA
                     DIPLNPLDLLPEKSLIIMKNPK"
     gene            complement(16785..17606)
                     /locus_tag="PLJ75_07265"
     CDS             complement(16785..17606)
                     /locus_tag="PLJ75_07265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPD05054.1"
                     /translation="MKLDNIVIAVSISSVMIVLAYLLFYYKTDAYVINNPVDIKHEEN
                     INITRLHKVPIILYHNIDGKGTYSIELKKLKNQFDLLKKHLIKVISLQTLESHINGHN
                     PFNQKVVSITFDDGYRSMYTKLMPLADSLNYPFTLFVYTDNIYYKSKNNLTWEMVKEL
                     HDHNIDIQCHSQSHPDLVKLYNKRTMQSKYELYREIYLSKKILELYLQKPIYYFAFPY
                     GRYTIELTELCRDAGYKRVFSTDYGPNIVIHDNFCLRRHHIKSNYRNEKVLTIAW"
     gene            complement(17593..>18395)
                     /locus_tag="PLJ75_07270"
     CDS             complement(17593..>18395)
                     /locus_tag="PLJ75_07270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD05055.1"
                     /translation="YITGGSSGIGLAFAKLAIQKGAHVAIIARDKAKLEKAKYEIKTC
                     CSNPHQRIEAFSVDVSNAGRVYKAVPAMIKKFGKPDILVCAAGLGFSNYFEKISDGDF
                     KRIMEINVFGIWYTLKAFYPYMAGRKTNIVLLSSLAGLIGIYGYSAYGTSKFAVFGLA
                     DCLRGEFKEKGICLSVLCPPEVNTPFIAEELKTIPKKTKRIKKMTGMLSAETIAKKIV
                     SAIQKNKYMIVPGTMSKITWLTHRMFNGYTTRIITDGIIKFLGNDETR"
BASE COUNT         5869 a         3917 c         2717 g         5892 t
ORIGIN      
        1 aaccttcact cataagtgcg gctatttgct gtatagcagg aggaatatat cctgcaggta
       61 tctcttcagt accaatctcg caaacaaagt taatcctctc catatccttt tatcctactt
      121 tcgtttcttt tttaattcca cataaggatt tatatgctga cctttcctga taatttcaaa
      181 gtgtagatgc gggccggtag agttgccagt attaccactc ttagcaataa tctgcccctg
      241 ttttacatat tgaccttctt tcacaattat tctggacaga tgcccataca atgttctatc
      301 gcccccggga tgagttacga caatcacctt accatatccc cctaaccagc ctgcatagct
      361 tatccgccca tatttgctgg catgtatcca ttcatagttt gctctaacat caataccctg
      421 atggaactgc ttatcaccat atacgggatc tttacgccat ccatagccac tggtaatata
      481 ttgattgtta acaggccata gaaacccctt gaaaatatca agcggctttg catcagggat
      541 aaagatatgc tgcccaacat tgataaaatc aggattagct attacattct cagcaattat
      601 tttttgtatc cctacattgt actttctggc aagagaagga agcgtctgac cgctttgtac
      661 actaactatc aatccatctt tattggggat ggttaaaacc tgtccaacat gcacaaggtc
      721 atatgattcc agcttattac atccacaaat cgtatccatc gaaacaccat acattccagc
      781 aatctcgctt aatgtttcac cctgctttat agtatggtgt acaatcttta atatggaacg
      841 ttcacggggt tcctcaaatt ctttattggc aatggtcttt ttcttaattt catcatacca
      901 tgaagcttct gaaaaaaata tatcgttaat accagccaaa ataaaaagca caacaaggca
      961 caaacaaaca gcagcaaatg ctatccccgc tctcctgaaa ctgcgccatg aaatgctttt
     1021 aataaaggca ttgtttttcc gtctgtggat aataattatc tcagatgata aaagtgttat
     1081 gcttatatca cctactatat aattatgtaa atgccacgat ctcatagatt cattatcggc
     1141 aacaaagaat cagtcgtcat ttttcagaac ccttcttgct gaaaattccc gcagcctgcg
     1201 gcgttccagc gtacgattct tcaataagct tcttcacatc tttcttttgt ccacctgtta
     1261 tggtaaccac accctgagaa accacaccac gctgtacagt cagtattggt gtttcaatat
     1321 cacccagcac cttggctgtt tccagtaatt taacctcttc atcagcaaca atattcccaa
     1381 tgacagtccc tgcaacaaca acatttttgg catgtatatt tgtcttaacc tttccggttt
     1441 cacctactac aagctcctca tcggttttga tctcaccttc aaatttccca tcaatacgaa
     1501 gcgaaccact gatataaaat ttaccttcaa aaaccgaacc ttcgccaata gtactattca
     1561 agctatcact tttaccagcc atacgtttat cacctcttgc ttattaattt ccttgttgca
     1621 ttattttttt gaatgtacat gtacttttga attattgcaa ttttatttca actatttttt
     1681 attgcaaatc aattattcct gtactatttt tactaaaagc aatactatta tgtgcgatag
     1741 ttactgataa ttaactgcaa cctgatgata tataatatat gaacatatta caaacaccca
     1801 tcgttgtcaa tgagatattc tactccctgc agggtgaaac tacccgtact ggatttccgt
     1861 ctctttttat acggctggca ggctgtaacc tcaattgcag gtggtgcgac actgcctatg
     1921 caaggaccgg ttatacaatg actatcgcca gtattgtaaa aaaaatacaa cagtacccat
     1981 tagccaacca tattaccatc actggtggcg aacctctgat acagcagggc acgatagctt
     2041 tgctgaatat tctttgcaca cataactatc ctcttcagct tgaaaccaat ggcagtattt
     2101 cactggaaaa agtaccgcaa cccgtacaca ggatagtaga tgttaaaaca ccttcaagcg
     2161 gcgaggaaaa gtcatttaac tttacaaacc tgcaattatt gcagccacac gatgaaataa
     2221 aatttgtgat agccaatgac aatgactata attatgctaa ggagtttatg caaaaatatt
     2281 gtgccaatac atgctgtgtt attaactttt caccggtagt gttaaccatg aagccgcaga
     2341 cacttgcaga atatattctg cgtgacagat taccggtacg gcttaacgtt cagttgcata
     2401 caatgttatg gcagcaagaa cccaaacatt tttaggagca agcgatggac tacgtaggac
     2461 atatcatacg ccccccttca gaagcgtata gtatgattat tcaggtaacc gttgggtgct
     2521 cgcacaacat gtgcaccttt tgcggtacct ataaggatca aaaattttat gtcaaagata
     2581 tgcagcaggt gaagcgtgat attgatgagg cttcgcggta ccgctttgat cgtgtttttt
     2641 taaccgatgg tgatgtgctt atactgccaa ccgaaacatt gctggattta tttgcctaca
     2701 ttaaaaaaaa gaatccgcat attgagcgca ttggtgttta tgccaatacc aaagccatac
     2761 taaaaaaatc agtggcggaa ttaaaacaac taaaagatgc cgggcttggc attgtatacc
     2821 atggcattga aagcggcaat gaagaagtac tacgcaggat acgcaaaggg gcgcttccgc
     2881 ataaacagtt agaggcagca caaaagatta aagaagcagg tattgcgcta tcacaaacag
     2941 tgctgctggg gattggtggt attgatttaa gcatccccca tgccatggac accggcaaac
     3001 atttaggtgc catgtcaccc gactatgcat cggcattaac cgttatgctt ttacccaata
     3061 cacccttata ccgtgactat gtacagggca aatttgtatt gcccgataaa tttggcttac
     3121 ttcgcgagct caagctcatg atagaaaata tggatgtaac cagtacatgc tattttacat
     3181 caaatcacgc atctaactat ttacccatta aagctacatt gcctgacgat aaaatacagg
     3241 ttatttcgtt gcttgaccat attatcaata caaaagacga atcaatatta aaaccagaat
     3301 atctcagggc attgtaagga ggggatggat catggataag tcaatggaag aggctatacg
     3361 ttttattagc tttgaacttc aggagagccc gggcaccgat attgcaaagc ttattgagaa
     3421 agccagtcag cagtttgacc ttacaccaat gcaaaccgaa tttctggtta acaaatttat
     3481 attaaataaa tagtaacgct ctgcaccgat agaatgtaca gtatgaagta tatacaccgt
     3541 acattgtcat tcaggtcttt acccggaatc caaatatcta tcgttatatc ctgaatcagc
     3601 gttaaggatg acatccaccg ccaattttat ttcatatgga tggcaacacc catgccataa
     3661 aaaactcctc atcttcaggt ttactgaaaa tgaggagttt atggcaaagc ttttatttca
     3721 aaatcaatct actcttcttc acctgttaat ttatccagta ttttcttctg ccctcctgca
     3781 tatactgcaa gcttctcacc ttccaattca atcttaccat cgtttgccag tttctttcgc
     3841 catagcactg ttccatctaa cttagcacag aagatataat cacctgatgc aatataaatt
     3901 ttgtcatcag cctttgttgg agggcttaca accttgcctt cccattcaac ctttccattc
     3961 tttacaaagt aaatttcata tcgggttacc agcagttgtc cttccggttt tggaatctgt
     4021 ttatccttct ttacaacctt tgcaacttct ttaactacct cttttggttt ttcttcaacc
     4081 tttatgattc cctgcttttc atcattaaaa tcttcaggtt taaatttttc caccttatct
     4141 ctacttacca caatctcctc tttagctact gcaattacct tctctattgc tttaggcgac
     4201 tcaccttcct ttgcaataat cacttcaacc ttcttttctg ccttttttac atcatccgct
     4261 gtaataatta ctttttcttt ttcctcaaca acgggagcta cttcaataat tttatcaaca
     4321 tgttcttcaa ctgcttctac cctctttact acagccacct tcccttcaaa aaccttgata
     4381 cgtgttgttt ttttagcatc agcttcaacc gaaaaatttg tgccgcgtac accagcaact
     4441 gcggttggag ttttcactaa aaaactttca ttttttaaaa gctttttagg tttgcatatc
     4501 atcttcccaa tatccagacc aagtgttgtg ttttcaatgc catcctttcg caaaaattgt
     4561 gccataatag cttttgattt ttcttttatc ctgattatgg aatcaccaat ttttatatca
     4621 catgacgaaa gtgcacctgt ttcaataata tccctttgat ttatcgcttc gccgataact
     4681 gctaactgcc cattcttctt cacatcacct atactgaacg ctattgatgc ataatcatca
     4741 accttctgct ttgcgcaact gcctataatg gctaaactta taattaccaa atatactaaa
     4801 ccccgtatca ttgtatatcc tccattaaag taaagataaa atattgcaca ttttatagat
     4861 ttgaacaaat acaacaaact ataaccaacc ttcttaatta caatcattta agtatacatg
     4921 tatttgtaga aaaaatgcaa atttttttta taatttataa aaaatcattt tttatatatc
     4981 actttcccat aatctctgaa atttttgtaa acggataagg attatacaac aaacttttct
     5041 tttacaattt gagatagtaa atacctatta tactatttat acaaataata acttccttcc
     5101 cttgtattca atacaatcta aatcaatacc ataaacacct ttcattattt caggattaac
     5161 aattttggaa tcaccatcac catagacata tccctctttc aacactataa atttatctgc
     5221 aaattgcagt gctctgttaa tgtcatgaaa cactattaac gtaattaaat tgtaattgat
     5281 tgtataatac cttactatct gtaaaatttc aatctggttt ttgatatcca gatggttgat
     5341 aggttcatcc agtaataaaa cctcaggctc ctgcacaatg gctcgtgcaa tcaaaacctt
     5401 ttgtatttct ccaccactca attgatttat ggttctaaat gctaatgaat ctatattaaa
     5461 attattaatg gttttctcaa caatttccat gtcatgcttt gttggttcaa atgatatata
     5521 tggttttcgc ccaagaagta ttgattcaaa tacgttgcat gatgggaaac tattgcctga
     5581 ttgaggtaga tatgcgatag attgtgcgat attgtttttt gtcattgcat taatattcat
     5641 tgtatccagg ataatagcac cgttatcagg ttttattatt ccatctattg cctttaaaag
     5701 cgttgattta cctgaaccat ttggaccaag taatgctgcc actgtacctc tatgcaattg
     5761 caacgaaata tctttcaaaa ctatttttga atcatatcca gcaaaaagat gttctactct
     5821 taacactata acgtactcct tcttaccagt agataaaaaa acattggtgc acccagaaat
     5881 gaagttataa tcccaaccgg aagaattact ggtgatagaa gcatttttgc gatactatca
     5941 gatattaaaa ggaataaagc gccaaacaaa gctgaatatg gtataaggaa acgataatca
     6001 ttgccaataa tcatccgaac aatatgcgga gcaatgagcc caacaaatcc aatgatacct
     6061 aaaaaagcag tacagagtga aactatcagt gcgcataata acatccccag taagcgtaag
     6121 gatgtactat ttacacccaa actttttgca acatcatcac cccataataa tgcattataa
     6181 ttccatcgat gaatgtaaaa ataaacaaaa caagctataa agcctatagt cataatcaaa
     6241 ttctctaccc atccggcttt tccgatatca ccaaatgtcc agaatattgt tgccgaaacc
     6301 tgaaagtctg tagcaaagta ttgcagcgcc attgttgaag caccaaaaag actgctcata
     6361 gcaacacccg ccaatacaat tgcttccggt gatacctttt taaccaatga caaaagaagt
     6421 attattgata cagctacaat agcacctgca aatgcagcaa acaccaccat atagacattg
     6481 aaaacactca cagattcatt gccatatgaa tgtgtaatcc ccgcacccaa accaatgatt
     6541 gcaactgacg caccaaatgc tgcaccatga gatataccca gtgtaaaggg agatgccaat
     6601 ggatttttta aaatattctg gataacacat ccagaaacgc ctaacgatgc tcctgcaatt
     6661 acagcagcaa cagatctagg taatcgaata ttgtatatca catacgaaag atcattttgc
     6721 caaaataatg ttgtgataat cttaccaaac ggtatatgaa gcgaaccagc agtaagagaa
     6781 aacaaaaaag cagcacccag acaaagcaga aacacaaacc ctatccccaa ttttacatat
     6841 aataactgta aatattgttg cattaatcca gatttaatgt acggtgagtc ccgcattggt
     6901 aaatttaact ttgccatatc ctttataact cttttttaaa ttatcgtata catccatacc
     6961 aacaaattct ttcacaatta ccctggcctt ttcttcagta tcaatatcat taaaccgttc
     7021 aggataaagc gttttcccaa taaaatatga attagcaatc ataacttcta tatttgttct
     7081 ataaaaatta tagggatata cagtatatac attattttta acaactgctt ttaatttttt
     7141 ataaaaacca ctgtttttct tgtaatcaat ttcaatgagt gcaagtccag cagcatcaat
     7201 aaataaatta tcgcaattcc acacaataag cttttccata tcaataaaga gatgtccctt
     7261 tttgcccaat tcatcggcaa cattcaaaac gtttagccat tgtaaaggtg gataaaaact
     7321 ttcagtgctg gttattccat gtgcaccacg tgcactcacc cctcctacat atactgatac
     7381 tttttttgta ttatggaccc tttgctgtaa atcagcaatg caggtatcaa tatatgtaca
     7441 tagttgcaac gctcgtactt gtttacctaa aatatttccc ataaataaaa atgttttctt
     7501 tatatcatca atagaaaaac catccattcc cgttgcgccc gatatgacaa tacatggaat
     7561 acctgtttta cgctgtatta tttctgcatt gtttgcttca aatgtaaata ctatatcagg
     7621 attaacctcc ataagccttt caaaatccgg aagcctgcct ggaccacctt cgcctatgcc
     7681 tggaatagca ccaactctgt ctgctattgc ggtccaatac attctaccat ctgcattaat
     7741 agtgcgcata gagtttcttt tctcaagctc ctcaacccca actactttat caaaagcctg
     7801 taaatagacc acatatcgta aagaagcact caatgccact acccctttaa tttcttctgg
     7861 aatagctacc ctcctgcctg caaaatcagt tatcacacta ttacctttag gtaacctggt
     7921 atcacacaat atacaaaata agcatagaac gcaacagcca attgttttat atagatccgt
     7981 attcatagta tataaaagca actcccctgc ttattggttt atgacatata aggaatatac
     8041 ggtttaatat caagtaccgg cgtaccatca aacatatcta tccgtttaac ttttattgta
     8101 aaaccatgta cttctaaaac ttcaacaatg gataatccaa taggatttgg cctaactggc
     8161 gaacatgtac tgaaaacacc aaggtactca tcacaatgtg gcggctttac aatgagcctg
     8221 tccatggtaa aggatacagc tctgtcaaaa agaaataata ccacaatgag gttgcctgcc
     8281 tttatatcgt gtatacccag cgcataatcc tcatcaataa ctatctcacc ttcaagctct
     8341 gataatgacc aatggcgcgg aacatcttta tgacggtgtc taatataccc aatgggttta
     8401 attggatagc ttattgattc catttcacct cttataattt aatgagtaaa aatatttcct
     8461 attatagcac gtacttcttg cgcagccgta ctaactgcac ccatcgggca agcgcaccct
     8521 tccagaggat gatatatttt tccaataaaa ataaaagata atggaccctc ttttacaata
     8581 tagtcatcat ctatattgtt agtatcaaac tgttcttttt caataatgct catttcaggc
     8641 tcatttttgc cattcctaaa tcttttgagc atcgtctgct gtactacttt tttcccacct
     8701 aagtattcca tgaaagattg tggcattttt gaaaaaccaa acattcgatg cagtcctgta
     8761 ttagattcat cagcatcgac tataagaaca tggcaatttt tatcaatcag agcttttgaa
     8821 attagatata ccatcaagct tttaccaaca ccaccctttc cacaaattgc aatttttttc
     8881 atacgttata tcccctgtat actattaatt taaaacatac acgacacaga accttttacc
     8941 gaaaacggtg cacctgcata ataacttgtt cccgataccg tatcatcaga agcattaatt
     9001 accgaaacat attttttgtt gaatatatta tcaaactcta acgaaaatgt tacatttttt
     9061 aacatgtgta tcttatcttt acaataactc acctttacgt caaaaacact atatgcatct
     9121 atctgctcat ggtgcataat atcaccatat cgttttccta tatagcgcag cattggtact
     9181 aattccagct gcttatactt cccgataatc ccggctaaaa caacccattg tggtgtatca
     9241 acaatctgcc tgccatcatt atcataaaca gtaccctgat aggtactgtt gccatcatag
     9301 acaagatgat tataggttgg attaacaaaa atcgtagccc acctagaaac aaagatactt
     9361 gttcccaatt cacatccata tccttttgcc tttcctgcat tttgctgata gcttactgcc
     9421 ttaccggtac cggaatcggt tactcgcgaa tctgtcacat ttactaaaag atttttatgt
     9481 tgtgatacaa aaactgttgg attaatctct aaataatggc gttttatcct cacaccaaca
     9541 tcaacattat cagattgttc aatatcatat cctttaaaaa gatcatttaa tgttatacct
     9601 gcagcctgaa attgtgtgcg cagtctatta taaaggtttt gcaaaggcat atatgcatac
     9661 ggtcgtataa aatttttacc gtaactggaa tacacttcaa aatatttagt aaatgaatat
     9721 gatactccca tcgtgggaag cattatatca tagattcttt catcccggtc taaatctggt
     9781 gctcttgtca acgctggtac actacccgga tatgttgttg tatatcctcg ggaatctgat
     9841 tctttaaagc gaaaatattt tactcctacc tgccacttaa aattttttat ctccccggcc
     9901 agttttccgt acgggctatt aataaaggta tcaccgctgg tggtatacat accgtatcct
     9961 ctgtataaaa gtgaaccgtc attttggatc caataatttt gtgtatatat attcatatcc
    10021 gattgttcaa aatgatagcc tgcggttgcc ttaatcattg ataaatcaat gctaatctgg
    10081 gaaacaaccc ctttcctaac aatatctcgt gtgcgctttt gcacgcctgg ttgattctgt
    10141 atattcgcac catctctgat cgtagcatct tcatttgtta tatacggctt tatcacgact
    10201 gagaaatctt tcccatgagc attgattaat gtaaaaaagt cgcaatttgt atgttcttct
    10261 tcattaaatt tgtaataaaa catatcctcg ccagggatac cggttaaccc accattaaaa
    10321 tcatatttat aataatcatc taaatcctta gcctgctcat aggataatga gcgatattta
    10381 taatgatgta ttgtattata attaccccac aatgaaatag tcatgtactt tcccataggt
    10441 tgaacaaaag taacgtttgc attatgccgt ggccctaaat ccccagggcc tttccattta
    10501 tcctctttag tgcatgaata tgaaaccgat aatctactgt cagccggacc aacttttccc
    10561 gaatcaactc gtacaaacat cttcatgaga tcaaaactac cataagaaga aacaagcgca
    10621 atcccaaacg tatcctgcgc ccatcgtggc tttagctcaa ccgcaccgcc tctatcacca
    10681 acacccacac ctaaatcagc agggacagcg cccttaaaca cagcaatgct atcaaagttt
    10741 tccatatcat acacataagc cctgggcccc atcggattac caccatagtt aggtatacca
    10801 tctacggtca tcgcacccat aaatcctcta acgccacgca ttctgatgct gttttgctca
    10861 gaaaccatat taccgggatc aattccttca aagaccaccc ctggtactat acttattgat
    10921 tcatatacat ttgctttacc cttctcacct ttaagctcca tccctttccg tgtaacctcg
    10981 gtcccggtat agaccgtttc ccctgcctgt tttgtaggga taatcaactt cgcaccctca
    11041 actactattt cttctaatat aaccattcca ttgttttcat catttgtttt atccgcttcc
    11101 gtttgcgtct gggcaaatac catcgtcata cccatgcata taatgatgcc aataaaacta
    11161 aacactttaa aaccatccat ctataccttc caaaaaaaca ataattatga taatattgct
    11221 acaagtatgc taataatagc acattaaaaa attatgtgtt atttgtcaat aatattaatg
    11281 gtatattagt agcaataaat gctattaata caatattaat aatattatct gtatattata
    11341 gatataatat tactatagcg tatagtaaat taagcttgac tgctttctcg ttatttttat
    11401 cataccattc atgaatgatg attggtataa tactgccata ttacaaagca tcattattgc
    11461 cgatgcattt gcaagccccc ttgataacct ttccgcccag cacattaagg ctacctttga
    11521 tgatgttacc gaatatgttg acccggaacc ggcgttgaag cataaacttt atctgtggaa
    11581 aaagccggga ctttatacat cgttatcgca atactgtatt ttgctttgcg ctataaactc
    11641 gcaaaactat acctataatc atcaactggt cattaatttt ttacattcac tgggcgatag
    11701 ttctgcatat ttcaggcata ctccccagat attgcacaac cttggcaatg cctatgatgc
    11761 acccactgca gaattgctgg tattgatacc ttcattgcta ttttgcaaac gcgaaccaac
    11821 gccacaaaat ttgttgcact ttatccttgc tcacaataaa aatgcggcaa cctgcgcagc
    11881 ctgcaccttc ttttatctgt tattgaaaga aattataggg cgacagctca gtacattcca
    11941 tcctgagatt ttaaacgata tatcgtcaca aatggcactg cttatacaac aacactctgc
    12001 atcaatcttt gaacatggag caaacccgca ggtgataacg caagctgcaa gcgacctgca
    12061 tgcactgatg acaacgctac cgcttacaag cgatgaagta accttcacac ggcatattct
    12121 taactatgcc aataagtgga gcaacaacag ttttacccgc ttaaccgtca atcatccttt
    12181 tgcaattttg ccatttgctc tttactgctc atattccaca ccatataatg cagtattttc
    12241 ccatatacac cacggtggca gggtctcggt tcttttgcca cttattgcag caatttcagc
    12301 ggcattatat ggccctgaca gtatccctga acatttaaag gaaacactta taaacaaaaa
    12361 aaggatatac cagttgctgc agtttttgca tgaaagctca atcaccatga actatctaac
    12421 agaatttttt caaaatgaaa tgaagctaac gcaaaaagaa aaggatgagc tggaaagtaa
    12481 actaaaacat agtaagcata ttaaagaaaa accaaaaaaa cctgctgcgg ataaacacaa
    12541 tgccatgaca aaccatgtgg ttgaaagctg gacaaaactt gataaagcaa aatggaaaaa
    12601 ggaaaagaaa aaacataaac cctattcaga ataatgcttg agatagttta gcattatctc
    12661 tttaaatcct gccagtgtaa gccatcgccc atgtgcaagc gcgattattt ccatatcatc
    12721 atccttttgt gcaaaaacac caccatatgc agaagcaatc tttttgccat attcaccctt
    12781 cactgtatca caaaaaccaa tgacatatac atcatcgctg ttataaactt tatcaaaaaa
    12841 ggtaatctta agttcaacag taactccgcc ctgccacacc gataggaatg ttgaccagcc
    12901 cattgtttca tcaagcaatg agcataacac cccttgatga atagaactat catccattgg
    12961 tacaaaatta ttaggggcat gtaaccgtgc aataagataa cttctatcaa gttgatagtc
    13021 agtaatataa aacatccttg aattattatt tcctggttta ttattaataa gatttactga
    13081 catgttgggg ataataaatg gtgttgcggc atccatgttt tgttttaata catcaatgac
    13141 atcattgtta taaggatgca cacgagtttc actataccgg caatggcaat agggattttg
    13201 caacgatctg ccaaaaatac caaaaaaatt tttttcatcg cccttttgaa caatgcaacg
    13261 cacagcatcc ccgcatgata aacgttcacc accaaatctg aaatgatatt cgtggggata
    13321 gagtgcagct gattcttttg caatatcaat gagttcaaca aaggctgcca ttgacaatcc
    13381 gccatgagga atccccggcc agccttcatg ttttttctct atcttgatat cagcaatgat
    13441 ggccatatcc tcaaaacggt acgacttaaa aataaagcca ttgataccac aataataaca
    13501 attattatgt ctcatatttt ttcttgtaat agaacaatca atccctatat aatagtttat
    13561 cccctatatt ctacagttat gcaattatct caatgaatga aaaaaaataa caataaagca
    13621 taaataatgc tatacaatac aggttgcaaa ctatatatta aaaatcaaaa caattttttt
    13681 ttaaactata aaatatttga caaaaatatt ttgttacaat aatctatgct tgtattgttg
    13741 aattataatt gaaataatgc atttttaaat cccggacagt acactattac gggtttgcat
    13801 gtaacaaaaa ttgattacgt atgggcacca taataaatca ggcttttcat atttaaaaaa
    13861 taattaaacc tgattttttc aatgattgtg cccgcagggg aacatcccga gtataattat
    13921 tagaggttcc cttataatga atagatgaat ggaggcatat atgtgcatat tcaggaaaaa
    13981 aaatttggta cttatactag tcaccgtttt aatctcaacg acaggtatcg caattgattt
    14041 ttcattccag ccaatagtta tagaaaactt atcacggtat aatgaagaca ggattgcata
    14101 tcaacatgtt caaaaagatg tatcccctaa tttagataat tcattttcag cccttttaat
    14161 aataaaatca aaaaaggttt atcttatcca ggacggctat gataatcctg agaatgtcag
    14221 taccaaaaaa ttacaatttg aaatggaaaa caaacctctt gatgatccat gggaaaataa
    14281 gataaataat aaacctgatt atgtacgtat aacagagcgc aagatagaat tattgaaaaa
    14341 ttttggtgaa gattttgtat caaaaaattt tggcaatttc tttttaagtg tacgcaatgc
    14401 attgataaaa aaacatgttg aagtatttaa aacacttatg gtcgaccgca aagaatcagg
    14461 gcttgtagtc acccgaacac ctcttccagt acctgcatat cttaatgcac ccgaaacgcc
    14521 aacaaaatat aaaatgaccg tttcaggaaa aactattgat gaaaaactat attacgcaga
    14581 ggatatcgat ggagatggaa taactgaaac ttttacggtc aatagccctg atggttttaa
    14641 ctggggctat aaatccggcc ccaacattat cctcatttta aataataaag atgaagaaat
    14701 taaaggttta attggtcagc tatgtaactg ggcatactat gggaccccag aagaagaaaa
    14761 agaaatattg cataatttcc ctactgatat ggatattatt aatgaattca agctcgaagc
    14821 agtaccaaca caacaaacaa aataataatt gattcattta tttatagtag aggtaggttt
    14881 ataacctgcc tctactgaat tactgttttg cttctttctc tgtacttttt taactcgtcc
    14941 agcagcctat aatcattccc ctgtatacca cctaatgtaa cctttggctt agaggttggg
    15001 ccatgaaaat ccgaacctgc tgtaataatc agattatggt cggcagcaaa tcgaatgaaa
    15061 taatcaatgt cttcttttgt atgatacgac gatatagcct ctataccagc tatccccatt
    15121 actgcaatct ttttcagatg ttcttcccct ttaacaaatt ttgggtgagc aagcacaggt
    15181 acaccgcctg tagctttaac cacttcaatt ccttcgtgcg tgctaattga tataaatgga
    15241 acataagcag gcatatgttc agccagatat tctctgtaaa aatacattaa tttgttatct
    15301 gattttttcc cattaaaata ctcttcaagg cgtttatcat ttctattatg tggattttca
    15361 aatacttcaa gcataatggc tgttgccgta ggaggccgat tattagcctt cctccataca
    15421 tttttttcat tcattacaaa gccaatatcc cgcaattttt gaatacgctt ttttgctatt
    15481 tcatggcgat agttacctat tttttttact atcgcgttga gcacaggggc attgttaata
    15541 aaatagccca gtatatgctg ctgggaacca tcttcataaa ttgtagttaa ctcaattcct
    15601 ggaataaact ctatgttata tttttctgaa caaacaattc cctcgggaat tgcatcaatt
    15661 gcatcgtgat cagtaatact gatagcactt aatccaatcg tttttgccat ctgaaaaata
    15721 tgatctacag aataatcacc atcttcacta tactgcgaat ggatatgcaa gtctatatat
    15781 cgataggtca taactggagc atagcacaca ttatttgggg tttttcatta ttattaaact
    15841 cttttcaggt aacaagtcca gtggattaag aggaatatcg gcgcatctaa tctcaaaatg
    15901 gaggtgatta cctgttgaac gtcctgtact gcctacaaaa gctatcgcct gacctttctg
    15961 aacataatcg cctaccttta caagcaatac tgaattatgg gcatagcggg taacataatt
    16021 atccctatgt tccagaacaa tggaattgcc ataattgcca aaatatcctg cataaattac
    16081 tttaccttcc attgtagccc tgaccatcgt gccctttggc gcaggtatat caacccccgg
    16141 atgaaaccgc ccccatcttc ttcctaatcc agaactaatt ttttcaggat caccaattgg
    16201 ccatataaac gcatcatagg gaacatttaa cggttgtaac gaaatcccct tttccattaa
    16261 ctgctggata tagatatcag aatagggaaa aaacacaaaa gatcttttaa attgtttttc
    16321 tttaatattg ttaatacaat acacatcttc aacagttata ccaaactggt tagccaaagc
    16381 ctttatctgc ttaaccatgt tcacttcaat ccatttacct gattttccat tataacgctg
    16441 tatattttca ttatctatga agtaaagctc acccgcaaat gaaaaagaaa caataacaag
    16501 aagaaatact ataacacact tttgtatatt cataacagta caatcctgta caaaaaaatt
    16561 taaaacaaca tatactccct atcttattaa taaaacgaag tattctattt atcgtattaa
    16621 tatagttatt tataagtaca cagtaagctt attgtataat ttttaatcac tactttttgt
    16681 caacaaaaat ttcatccttt accatatgtc aactgctttg tgagcgtatt cttagtcctc
    16741 tatctttacc tgcttgattt ttcccacagt ttatgagtag ttacctacca tgcaatagtc
    16801 agtacttttt cattacgata gttacttttg atatggtgtc ttcgcaaaca aaagttatca
    16861 tgtattacaa tatttggtcc gtaatcagtt gaaaatacac gtttataccc agcatcacgg
    16921 catagttctg taagctcaat tgtataacga ccgtatggga aagcaaagta atatattggt
    16981 ttttgcaaat aaagttctaa tattttttta gataaatata tttcccggta cagttcatat
    17041 ttactctgca ttgttctttt gttataaagt tttacaagat caggatgact ttgcgaatgg
    17101 cattgtatat ctatattatg gtcatgtaat tctttcacca tctcccatgt taaattattt
    17161 ttgcttttat aataaatatt gtcagtatac acaaaaagcg taaaggggta gttcagacta
    17221 tcagccaatg gcatcaattt agtatacatc gacctatatc catcatcaaa tgttatagat
    17281 acaaccttct gattaaatgg attatgtcca ttaatatgcg attccaatgt ttgtagtgaa
    17341 attactttga tcaaatgttt ttttaacaga tcaaactgat tttttagttt ttttagctca
    17401 atagaatatg tacctttgcc atcaatgtta tgatacaata taataggtac tttgtgtaat
    17461 ctggtaatat tgatattttc ttcatgtttt atatctactg ggttatttat tacgtatgca
    17521 tcagttttat agtaaaataa caggtaagcc agtactatca taacactgga aatgctcact
    17581 gctattacaa tattatctag tttcatcatt ccccaaaaat tttattatac catcagttat
    17641 aattctggtt gtatatccgt taaacattct atgggtaagc catgttattt tgctcatcgt
    17701 gcccggaact atcatatact tatttttttg tattgcactt actatttttt tagcgatagt
    17761 ttctgctgaa agcatccctg tcattttttt aatgcgtttg gtttttttag ggattgtttt
    17821 aagctcttca gcaataaacg gtgtattaac ttcgggtggg catagtactg aaagacatat
    17881 tcctttttct ttaaactcac ctcgcaaaca atcagccagt ccaaatactg caaattttga
    17941 tgtcccatac gctgagtaac cataaatgcc tattaatcca gcaagcgatg aaagcaaaac
    18001 aatattcgtt tttctcccgg ccatataagg ataaaaagct tttagcgtat accaaatacc
    18061 aaaaacatta atttccatta ttcttttaaa atcaccatca ctaatcttct caaagtaatt
    18121 tgaaaatccc aatcctgcag cgcaaaccag aatatcaggt tttccaaact tttttatcat
    18181 agcaggtact gctttatata ctcttccagc attactgaca tcaacagaaa atgcttcaat
    18241 cctctggtgc gggttgctgc agcatgtttt tatttcatat tttgcttttt ctaacttcgc
    18301 tttatcccga gcaattatcg caacatgtgc acctttctgt attgccaatt ttgcaaaagc
    18361 aagccctata ccgcttgaac caccggtaat ataaa
//
LOCUS       DAOOJH010000044        18194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3022_length_18194_cov_9.979988, whole genome shotgun sequence.
ACCESSION   DAOOJH010000044 DAOOJH010000000
VERSION     DAOOJH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3022_length_18194_cov_9.979988"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="PLJ75_07275"
     CDS             complement(<1..1349)
                     /locus_tag="PLJ75_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPD05056.1"
                     /translation="MNYWQEYNAKLTTPDNAVKVVDCGDVVDYGFFNGKPVILDQALA
                     KRAPELKDVVIYTAVTLPPVPEVIKYPQSFTYIDWQWSKLTRLLHTQVEAAYYCPILY
                     HRAPFYYRYLLNQDPHENITPGYRSYYYNIPEKSKNVKWIAILQVGPMDDQGFFNIGP
                     QNSETSAKIDAADYVLVEVNKNMPRCLGGSEEAIHISRIDFIVEAPPEQMIFGAPEVP
                     PTEIDKKIAEHVMQFLYDGCCIQLGIGGMPNMVGKLIAQSDLKNLGGHTEMFVDAYVD
                     MIESGRMNGAKKNIDRFKCVYTFAIGSQHMYDFMHDNQGLASYPVDYTNDPKVIAQND
                     DTVSICNAIQVDLFSQVNAETLGSGQVSGNGGMWDFVLGAQWSKRGKSFICLSSTHTN
                     SKGELQSRIVPQLPLASTVTIPRQMVDYIVTEYGAVKLVACPTWMRAEKLISIAHPQ"
     gene            1779..7331
                     /locus_tag="PLJ75_07280"
     CDS             1779..7331
                     /locus_tag="PLJ75_07280"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPD05057.1"
                     /translation="MKKTSKMLISTVIGIFMVSIVFAAALVIESNEIKLVIDYQVEKP
                     YTEMALSFSKDIFVLSDVDSMVKDVNDKLHITFKPDIEYTIKPLDTRSCIVEIYNPKP
                     ANEYHFTITVKGYDKKAITITCNNSEIEQGKDYYFRTPLLKVVEYHRSSEMINTPIVL
                     RFNFPVSLEVLKQKLSIDPKISVAVEYDKDDTTKKTVIVYPANEEKAQKYSLTINKSM
                     VPVGGVLGMESHFSFTYNTFYPLELMKVESAYGRSGSFYPDYPVEFTFNNEIAIPEGH
                     SAAEYVTVSPNPGGVQVSVYGDTLSISGNFIGNERYTVTIAKDIQDIYGQKLDEPIVE
                     EITFEHAFSHFTCPENYMLVESYMEKIVPMHMVNVSSFSCKYLSLVDERDIRKYLENP
                     KEYFKEYATVKHYTVNWAWDVYKTVKFDFSKLHAANHGFFVYEITPSMENPYNSESNT
                     FHGVMQLSDIGVTVKKTKNNVFVHVRSLLHNTPLQNASVYCTGDSPIGKTDKKGVAII
                     KNTGCSVYTIKHEQSIFYYSMSNSSYHDEDNSNTAIQLPEKHYGSSYFINTPKIQLFT
                     DRYLYKPGEIVHVKGIFRYRNNDQWSLNPPLSPVKDIIVTVYNSRHEEIETYTKSLSP
                     TGGVQWDIPIKGNYPTGYYRITITAKNKDYNINGGIHFQVEEFKPAKAEMKIIPEKLK
                     YHWGEMLAMDVFGWYLFGAPVNNDVEYTVSLTPVIYRSESYPDYSFSTQLYYYDEYDE
                     YTPSSTTIATGRVKVNEKGIARVFVPLKAMANVPNADIVITAKTYLSDDSPVFGAKAG
                     LQVFEPVHVGIRVPRYFNNTDSPVTVQLVALNSSDKVVEGNTVKLVVKKHEWKSFQIA
                     GVNGRLQWEYKNIATDVYAHNYVIGKKEVAVAINDPGYYTAEVYDSSGKRKFAVKSFY
                     SIGKGEAGWRVNDDESVDVETDKSSYNVGDTAMLLVKNPFEKASAMVTVEREKFFDYF
                     EVPATTSIVMIPIKITEEHIPNIYVSVMVYTPRTGFNKVKHGEDLAKPKCYIGYASIK
                     VVPREKKLQVKVKSDKKQYKPGEETTIEINVTNYKGQPINGEATISVADRGVLNLVNY
                     TLPNPLDFFYAPRSLAVTTFDVRQIILGQRYIKEKGELIGGDGGIASMGMIVPRANIR
                     FSAYYNATVKIEKGVARIKFKLPDNLTSFKSMVVVHTNDSRFGYGETTFEVKTPLMVL
                     PTFPRFARVGDAFDAGALVFNYSGIKQTITVSMELSGGIKCQDGKLSSIKEIDLNNGQ
                     STELTFPIVVDNANQSTFVIKAKADKHTDGIKDSIAIKAPNIYETVAQYGRITKADDS
                     AKHTIDISKNIIPYVSSVNVFVSPSAFSELKGSLDYLVEYPYGCLEQKASRILPLIIG
                     EDIIIQKNLLKIKSKEDLKNTVATILKEFPGYFKNDGFSYWQDSSYTSSYLTVYATFI
                     MTMAKKQGYTVDAVTFDKALATVKRFATKGVIFSNEESFFSTYYKYLTLSFAHYVAAL
                     NGVTSENQLKLVYSVMNSKYEDMIAGYAYLLKAVALYPDSNFKQSMTRMIAQKFFSAM
                     RTESGYVYFKDASDWGYFYYDDVIVTSLILQSLLEAGVPFDNDYKVVNYLLKQRKGYA
                     WLNTHENAIVWWALNTYFTKYEKGKPDFAVTVLFNAKELLNALITDTSTTYSGLYAIT
                     HQDSGSRSLQLSKKGEGVLYYTFRYTYIPVYDKMISKNMGFLITKRFKDVKTGNEVTA
                     FKKGSEYLVEIEVYTPKERGITVLDDQLPAGFEPVNITFATEEKMTAEKRNAYYADWG
                     TFNHYEQYNDRVVYYADFMHTGNHTVVYKVRATNSGVFKLPACKVEEMYNPEVFGIMY
                     YTNAVQVQ"
     gene            7328..9616
                     /locus_tag="PLJ75_07285"
     CDS             7328..9616
                     /locus_tag="PLJ75_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD05058.1"
                     /translation="MIAMKPLLTLECRYRIKKIIKLSSGVIFLAIVIYIVFPYKRDYL
                     ASSNKLPVVIYDRNGTVLMEVAKGKSGLCQAISIQQLPEDFISLLLFSEDRNFYHHWG
                     ISLKSIARALYQNGKAMRIVSGGSTITQQVVKSKKEILRNTVLTKMLEFIEAVRMELH
                     FSKDEILQGYINEVYLGNNIYGFEKAAQIYFGKHINECNLLEAGFLIAIVKAPERYNP
                     YQNPETVVQSAKRLLRNACHMGYVKISPFELEVYTNSRIALQYNEKKVIAPLFCLYAL
                     SQARRLFPDKDITHIYTTLDATIYSNVLTVMQNSMSMLKDNNALHAALVMIDNTTMEI
                     VAMIGSVDFFDYKKGQVDATLIKRQAASTMKPFAYALALETGMFHTSSIVPDVYTQFP
                     SKVGNYIPKNFSDSYHGPVRFAKALGCSYNIPAVYVVSKIGLVSYYNLLKKIGFDSIT
                     RSPSYYGLGLVLGNADVSLLELANAYTVFPRQGLFSKTIAIKKIRDAHGKEYTVQSAG
                     SARVLKQQTCYLISHILSEYKYKVDAFGLCSSINFPFPFAVKTGTSKDFKDNFIAGYN
                     KQYTFAVWVGNLYNESLCDLPAVSGAGIVLKDVLLYLWNAGYPFERFTMPENIRKVKI
                     CTLSGMVADTYCHSTDYELYSGNNQPCNKCNWHTKNATIVPSQFKEWARNKNYNVAHD
                     ASVQIVFPGNGVVFKIEKTVRKNLQAIPLKATANSNIQWYVDNRYIGEGSELIWQLSA
                     GHHVISIQAGNQRDAVNITVLE"
     gene            9798..11891
                     /locus_tag="PLJ75_07290"
     CDS             9798..11891
                     /locus_tag="PLJ75_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD05059.1"
                     /translation="MMHKLFYPDSIAIVGLSSRPNNIPRLTLENTLRWGYSGRIFGVN
                     PKSDDEQVDGIRMYKSIGALPEIPDLVYALVPAKFVPGIVEECGQKGVQWMAIPSGGF
                     SEYSEEGKSLAQKTVDTAKKYGIRFVGPNGLTVANVENGLCLPFVPILRPQFGGMSIV
                     SQSGGVALMMWNVIMDENIGIAKFASIGNKLDLDEVDFLEYLGSDPATSIICMYLESI
                     PRGSDFIKVADRINKPIVVYKSNTTQAGKKAAMSHTAAMSNDEDIINAAFEKAGIIRI
                     YNYHDFFAVAKAFKLPPMKGKRVMVMSPAGGFSVMTADLCEQAGFKFANPGKEFYESL
                     QKFSNAGVIQFSNPLDMGDIYDPAFTAHVLYSVMHNNNVDGAMYVSQRPQMPDRKNVF
                     SQMFLSDLSKEAWGAIVSSGKPLAICLLGMAKLMAQIKRSVNFPIFNSAEEMVRAMKF
                     QMKYWLRRSTPQMHESYPMSLDTVLRWITNHHGDIGEDAMELLEFIDIPVPQSGIAKS
                     EKEAVTVAKQLGYPVVCKVISRDILHKSDVGGVIVNVNGDEGVKQAYAAIQSSVLSHK
                     PDAHIEGIRISTMAPDGVDMFVGSKYDQSFGQVIIFGFGGIYVEVFKDIAYCLCPATK
                     EEIKQKLTSLKSYALLKGARGKSALDVDAYVDIIWKVSHLLASASHIKELDLNPVRVF
                     EHGCQVLDVRMRIVH"
     gene            11929..13074
                     /locus_tag="PLJ75_07295"
     CDS             11929..13074
                     /locus_tag="PLJ75_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPD05060.1"
                     /translation="MAMVYLDNAATSFPKPDTVIKAVTNCLTNYCANPGRSGHSLSIE
                     AARKVFETRETVAELFHAKDSRYVIFTANATMAINLVFHALLQKGDHVVVSSMEHNAV
                     MRPLRHWESQGIITLSLANADGNGFVDDDIGAFIQPATRLCVINHASNVNGVVQDIAS
                     IAGRIKEINPDVAVLIDASQSTGLIPIDIQAIKCDFVAFTGHKALYGPQGIGGLVINT
                     AKHLQPFIMGGTGSKSESQFQPQFLPDKFESGTLNLPGIAGLKAGIDYVKSRMDEIHH
                     NELLHIKTIVEGLSSIKDLKLFWSRSLHNQIGVISVAHNHIPCSEIGQRLNDEFSVFV
                     RTGLHCAPLAHETLGTYPNGTLRVSVGLFTNKSDVDVFCKAIEIISK"
     gene            13080..13331
                     /locus_tag="PLJ75_07300"
     CDS             13080..13331
                     /locus_tag="PLJ75_07300"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPD05061.1"
                     /translation="MDYCYLLLPSVHDAMKAEKLLLQSGCAIQVIATPREISHNCGVV
                     IKFSCAEINTVLLSCKELSAEQKIFKKNSNNVYYEIIPE"
     gene            complement(13318..13464)
                     /locus_tag="PLJ75_07305"
     CDS             complement(13318..13464)
                     /locus_tag="PLJ75_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05062.1"
                     /translation="MIQNSFDMKQWKMEQIKYFKKYQSTFPKNSKEYKILAESIRKLE
                     STLG"
     gene            complement(13793..15004)
                     /locus_tag="PLJ75_07310"
     CDS             complement(13793..15004)
                     /locus_tag="PLJ75_07310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD05063.1"
                     /translation="MRDAYIVQAVRTPGCRRGKGAFAQTRPEDLLVTALQGVIERAKV
                     DKKVVDDIMVGCAFPEAEQGLNVGRIAAQMAGFPDDTCGATVNRFCASGLEAIAISTM
                     RIQAGWCDVAIGAGLESMSIVPMGGNMPRPHPYWAKQNPDVYISMGITAENVANRYKI
                     TRQMQDEFAYHSQMKAAEAQKNKLYNEIVPTKATKFVPQPDGTIKKEEFMQDFDDGVR
                     SDTTIEGLGKLKPAFAAFGSVTAGNSSQTTDGSAATLLMSEEACRKYGVKPLAKVKAY
                     VVAGCKADEMGVGPAYAIPKLLKLVGLDIKDIGIFEINEAFASQAVYCAQQLGIADPK
                     EWAVDSKTRRINVNGGAIALGHPLGCTGAKLAAQIVNMMTKNGIKYGIESMCIGGGMG
                     AAALFELVEKW"
     gene            complement(15041..17446)
                     /locus_tag="PLJ75_07315"
     CDS             complement(15041..17446)
                     /locus_tag="PLJ75_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HPD05064.1"
                     /translation="MVRRIEKAAVIGSGIMGGGIAALLADTGVNTLLFDIVPFNLTDE
                     EKKDPKARYRIVQAGFDGLMKAKPAAFMDKSNASRISLCNLEDDFDKLKECDLIIEVV
                     VENLKIKQDLFQKLDKIRKPTAIVTSNTSGLPLKEMSKNCSDAFKEHFMGTHFFNPVR
                     YMHLLELIPGEKTKKEVLEFIADFGEKRLGKGIVWAKDTPNFIGNRIGVHEIMTAMKL
                     LESENITIDEADAIMGPAMGRPKTAIFKLSDMVGLDTIHHLAENSYQLLEKDERREVY
                     KVTPWFNKMVENKWLGDKTKQGFYKKEKTPDGKRLSLVLDPVKMEYREAQKPEFESVS
                     AAKKLPTAAEKIKAIVWGKDKAAQFAWKLTAYDAIYAANRIPEIADSIVEIDNAMTWG
                     YNLELGPFQAWDAIGVKESVERMEKEGMKVPENVKKMLASGATSFYKVENGKEYYYDL
                     VNGGYKEVKKSEAAISIFNLKGAGKEVKKTNSISLIDLGDGVFCFEFHSKMNAINGEI
                     VDFMLANIDYVKENGVGAVIGNQAGGMPGAFSAGGDLAYMGSLAKAKKWSEIDKFIAN
                     VHTSQLAAKYAPIPVVAAPYGMTLGGGCEVCLNADRIVAHAELYMGLVEIGAGLLPGG
                     LGMMNLWRKYLKSVPKAAKITDLAGLFLPCFMAVAQAQVTMSAFEAQKKGFLGAKDRI
                     VFNRDLLIGEAKKEVLRMVDEGYAPPKKEKFAVLGREAQGMVDAEMLNMRIGMYITPH
                     MEFIAKKIAWVMSGGDVPSGMEIDEAHWLKLEREAFVELWQTENTQKMAEHILQTGKP
                     LFI"
     gene            18027..18099
                     /locus_tag="PLJ75_07320"
     tRNA            18027..18099
                     /locus_tag="PLJ75_07320"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18060..18062,aa:Cys,seq:gca)
     gene            18104..18189
                     /locus_tag="PLJ75_07325"
     tRNA            18104..18189
                     /locus_tag="PLJ75_07325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18138..18140,aa:Leu,seq:taa)
BASE COUNT         5591 a         3389 c         3688 g         5526 t
ORIGIN      
        1 aattgaggat gtgcaatgct tataagcttt tcagctctca tccatgttgg acatgctact
       61 aatttaactg cgccatattc ggttactatg taatctacca tctgacgggg gatagtaact
      121 gttgaggcta atggtagctg cggcactata cgtgattgca actcgccctt tgagttggtg
      181 tgggttgatg ataagcatat aaagcttttt cctcgttttg accattgagc acccaataca
      241 aagtcccaca tgccgccatt acctgaaacc tgcccggaac caagagtttc agcattaacc
      301 tgagagaaca gatcaacctg tatggcatta catatgctta ctgtatcatc gttttgtgca
      361 attacctttg gatcgttggt atagtcaaca ggataagagg ctaatccctg attatcatgc
      421 atgaaatcat acatgtgttg tgaaccgatt gcaaatgtat atacacattt aaatcggtcg
      481 atattctttt tagctccgtt catcctgcct gattcaatca tatcaacata agcatcaaca
      541 aacatctctg tatgaccacc cagattttta agatcagatt gagcaataag tttacctacc
      601 atatttggca taccaccaat acctaactgt atacagcatc cgtcatataa aaattgcatg
      661 acatgttctg ctatcttttt atctatttcg gttggaggta cctcaggtgc tccaaatatc
      721 atctgttctg gaggcgcttc aacaataaag tcaatacggg atatatggat agcttcttct
      781 gacccaccaa gacaccgcgg catattttta tttacctcaa caaggacgta gtcagcagcg
      841 tcaatttttg ctgatgtctc agagttttgc gggccaatat taaaaaatcc ctgatcatcc
      901 attggcccaa cctgcagtat ggcaatccat ttcacatttt tacttttttc agggatattg
      961 tagtaatatg agcgatagcc gggggtaatg ttttcatggg gatcctgatt aagcaaataa
     1021 cgataataga atggtgctct gtggtaaaga ataggacagt aataggcagc ctctacctga
     1081 gtatgcaata gtcgtgttag tttgctccac tgccagtcaa tataggtaaa actttgagga
     1141 tatttaataa cctcgggaac cggcggtaag gttaccgctg tatatataac cacatctttt
     1201 agctcaggtg ctctttttgc cagtgcctga tctaaaatta caggtttccc gttgaaaaaa
     1261 ccataatcga caacatcacc gcaatcaaca acttttactg cgttatcagg agtagttaat
     1321 tttgcattat attcctgcca gtaattcata aatacgcctc cattttttaa aaattttaat
     1381 tcaaaatatt ataataatac aatattatat tgtcaataaa aatatttagt attattaaaa
     1441 attatattaa taataaatta tatatatacg atattattat aatgttgtaa tatatatatc
     1501 tggcattcaa ttattgaatt aatccccaat aatatattat ttgctttaca cagcagatgt
     1561 aactatggta acattatcta cttaataata caaatgatga tgttttaata gaagaagttg
     1621 cctgtggacg atgtatagca agccatagag gatgaaaata gtgcttttac tttttttaat
     1681 acggtggcat gctgctttac ttccatgggg aaggaaaaaa ttatatgata gccgatacca
     1741 taccatagta ttccacaaat gaaaaggaga aaattgttat gaaaaaaaca tctaaaatgc
     1801 ttatttcaac agtcattggt atattcatgg tcagcattgt ttttgctgca gcccttgtta
     1861 ttgagagtaa tgagataaaa cttgttattg attatcaggt tgagaaacca tataccgaga
     1921 tggcattgag cttttcaaag gatatttttg tgctcagcga tgtcgattct atggtgaaag
     1981 atgtcaacga caagctccat ataacgttta agcctgatat agaatatacc ataaaaccac
     2041 ttgatacacg cagttgtatc gttgaaatct acaatccaaa acctgccaat gaatatcatt
     2101 ttactattac ggtaaaaggc tatgataaaa aagctataac cataacctgc aacaatagtg
     2161 aaatagaaca ggggaaggac tactatttca gaacaccatt gttgaaggtt gtagagtacc
     2221 atcgaagcag tgaaatgata aataccccca ttgtgctacg atttaatttt cctgtttcat
     2281 tagaggtgct gaaacaaaaa ttgtctattg atcccaaaat aagtgtagca gtagagtatg
     2341 ataaggatga tacaacaaaa aaaacagtta ttgtgtatcc tgcaaatgaa gagaaagccc
     2401 aaaaatattc tctcacaata aataaaagta tggtaccggt tggcggtgtg cttggcatgg
     2461 aatcgcattt ttcatttacc tataacacct tttatccatt ggaactgatg aaggttgagt
     2521 ctgcctatgg acggagtgga tctttttatc cagattatcc tgtagagttt acttttaaca
     2581 atgagattgc tatcccggaa ggtcattcag ctgcggagta tgtgacagta agtcccaatc
     2641 ccggcggcgt gcaggtcagc gtgtatggtg atacattatc aatcagtggt aattttatag
     2701 gcaatgaacg atatacagta actatcgcca aagatattca ggatatttat ggtcaaaagc
     2761 tggatgaacc tattgtcgaa gagattacct ttgagcatgc attttcgcat tttacgtgtc
     2821 cggaaaacta tatgctggtg gaaagctata tggaaaaaat tgttccgatg catatggtca
     2881 atgtttcatc gtttagctgt aaatatcttt cacttgttga tgaaagggac atcagaaagt
     2941 atcttgaaaa tcctaaagaa tattttaaag aatacgctac cgttaaacat tatactgtca
     3001 actgggcatg ggatgtttat aaaactgtta agtttgattt ttcaaagctt catgccgcta
     3061 accatggatt ttttgtgtat gagattacac cgtctatgga aaatccctat aacagtgagt
     3121 ctaacacatt tcatggcgtt atgcagcttt cagatattgg ggttacggta aaaaaaacta
     3181 aaaataatgt ttttgtccat gtgcgcagtt tgcttcataa tacaccgtta caaaatgcct
     3241 ctgtgtattg cacaggtgat agccccattg gtaaaactga caaaaaaggt gtggcaatca
     3301 ttaaaaatac ggggtgctct gtttacacca ttaagcatga gcaaagcatt ttttactata
     3361 gcatgagtaa tagttcatat catgacgaag acaatagcaa tactgcgata cagcttcctg
     3421 aaaaacatta tggctcaagt tattttatca atacgccgaa gattcagctt tttaccgatc
     3481 gctaccttta taaaccaggt gagatagttc atgttaaagg gattttcaga tatcgaaaca
     3541 atgaccagtg gagtttaaat ccgccgctat ctccagtgaa agatattatt gtcacggtat
     3601 ataattctcg tcatgaggag attgaaacgt atacaaagtc actatcgcca acaggtgggg
     3661 tgcagtggga tataccaata aagggtaact atcctacagg atactatcgt ataactatta
     3721 cagctaaaaa taaagattat aatattaatg gtggtattca ctttcaggtt gaggagttta
     3781 agcctgcaaa agccgagatg aagattattc ctgaaaagtt aaagtatcat tggggtgaga
     3841 tgcttgcaat ggatgtgttt gggtggtacc tgtttggtgc gccggttaac aatgatgttg
     3901 aatatacagt ttcgttaacg ccggttatat acaggagtga atcatatcca gattattcgt
     3961 tttctacaca gttgtactac tatgatgaat atgatgagta caccccatca tcaactacta
     4021 tagctacagg cagggtaaag gtaaatgaaa aagggattgc cagggtattt gttcccctta
     4081 aagcaatggc aaatgttccc aatgctgata ttgttataac tgcaaagacc tacctttctg
     4141 atgattcacc ggtgtttgga gctaaagcgg gattacaggt gtttgagccg gttcatgttg
     4201 gtatacgggt gccacgctat tttaataata ccgacagtcc tgtaacagta caactggtag
     4261 ccctgaatag cagtgataaa gtggttgaag gaaatactgt caagcttgtg gtgaaaaagc
     4321 atgagtggaa atcatttcag attgccggtg tcaatggcag attgcagtgg gaatataaaa
     4381 atatagcgac agatgtatat gcacacaatt atgtcatcgg caaaaaggag gtagcagtag
     4441 caataaatga tccaggctat tataccgccg aggtgtacga tagttccggc aaacgaaaat
     4501 ttgcagtgaa gagcttttac agtataggga agggtgaagc tggctggcgc gtcaatgacg
     4561 atgaatcggt tgatgttgaa accgataaaa gcagttacaa tgttggtgat actgccatgc
     4621 tgcttgttaa aaatcccttt gaaaaggcgt ctgccatggt aacggttgag cgtgagaaat
     4681 tttttgacta ttttgaagtc cctgctacta ccagcattgt catgatacca ataaaaataa
     4741 ccgaggaaca tatacccaat atatatgtca gcgttatggt atatacgcca cgaacaggtt
     4801 ttaataaggt aaagcatgga gaggatttag caaaaccaaa atgctacata gggtatgcca
     4861 gtataaaggt tgttccccgc gaaaaaaaat tacaggttaa ggtaaaatca gacaagaagc
     4921 aatacaaacc tggtgaagag acaaccatag agataaacgt aactaattat aagggacagc
     4981 caatcaatgg tgaggcaacc atttcagtag ccgaccgcgg tgttcttaac ctggttaatt
     5041 acacgctgcc caatccgctg gacttttttt atgctcctcg cagtcttgct gttacaacat
     5101 ttgatgtgag gcagattatt ctggggcaac gatatataaa agaaaaagga gagctcattg
     5161 gtggtgatgg cggtattgcc tcaatgggga tgattgtacc gagagcaaat atacggttta
     5221 gcgcttatta caatgcaaca gttaagatag aaaaaggagt agcccggata aaatttaaac
     5281 tgcctgataa ccttacaagc tttaagtcaa tggtggtggt gcatacaaac gattcacgat
     5341 ttggctatgg tgaaacaaca tttgaggtta aaaccccgtt aatggtactg ccaacatttc
     5401 cgcgttttgc ccgtgttggt gatgcatttg atgctggcgc acttgtgttt aactattcag
     5461 gtataaaaca aacaataaca gtaagcatgg agctttctgg aggtattaaa tgccaggatg
     5521 gaaaattgag cagcatcaaa gagattgatc ttaacaatgg tcagtcaact gagcttacct
     5581 tccctattgt tgttgataat gccaatcaat ccacatttgt cattaaggca aaggctgata
     5641 agcatactga tgggataaag gacagcattg ccataaaagc acccaacatc tatgaaacgg
     5701 ttgcacagta tggaaggatt accaaagcgg acgatagtgc aaaacatact attgatattt
     5761 caaaaaatat cattccgtat gtttcttcgg taaatgtgtt cgtttcgcca tctgcatttt
     5821 ctgagttaaa gggaagtctt gattatcttg tagaatatcc ctacggctgt ctggagcaga
     5881 aggcttccag gatattacca ctcattatag gtgaggatat tattattcaa aaaaatttac
     5941 tgaagataaa atcaaaagag gatttaaaaa atactgtcgc aacgatatta aaagaatttc
     6001 caggctattt taaaaatgat ggtttttcat actggcagga cagttcatat accagtagct
     6061 atctaacagt atatgctacc tttattatga caatggcaaa aaaacaaggg tacaccgtag
     6121 atgccgtaac ctttgataaa gcgttagcaa ccgtgaaacg gtttgcaaca aaaggggtta
     6181 tttttagcaa tgaggaaagc tttttttcaa cctactataa atatctaaca ttgagctttg
     6241 cccattatgt tgccgcactc aatggcgtta ccagcgaaaa tcagcttaaa cttgtttaca
     6301 gcgtcatgaa cagcaaatac gaggatatga tagcaggcta tgcgtatcta ttaaaagctg
     6361 tagcgttgta tcctgattcc aattttaagc agtccatgac cagaatgata gcgcaaaaat
     6421 tcttttcggc gatgcgtact gaatcaggtt atgtttattt caaggacgca agtgattggg
     6481 gatattttta ttatgatgat gttatagtga catcgctcat tttgcagtcg ttgctggagg
     6541 caggggtgcc atttgacaat gattataagg tggtcaatta tctgctcaaa caacggaaag
     6601 gatatgcttg gcttaatacg catgaaaatg ctatcgtctg gtgggcactt aatacgtatt
     6661 tcacaaagta tgaaaaggga aaaccagatt ttgcggtaac ggtattgttt aatgctaaag
     6721 aattgctcaa tgcattgatt accgatacca gtacaaccta ttccggattg tatgcaatta
     6781 ctcatcagga tagtggttca cggagtttac agctttcaaa gaagggtgaa ggcgttctct
     6841 attatacttt tcgatatacc tatattcctg tgtacgacaa aatgatatca aagaatatgg
     6901 gctttttaat aacaaagcgt ttcaaggatg tgaaaaccgg caatgaggta acagctttta
     6961 aaaaaggttc agagtatctg gttgaaatag aggtatacac gccaaaagag cgggggatta
     7021 ctgtcctgga tgatcagttg cctgcaggat ttgagccggt gaatattacc tttgccactg
     7081 aggaaaaaat gacagcggaa aaaaggaatg cttactatgc agattggggc acattcaacc
     7141 actatgaaca gtataacgac agggttgtat actatgctga cttcatgcat actggcaatc
     7201 acactgttgt atataaggtg cgagcaacca attcaggggt atttaagctg ccggcatgca
     7261 aggtggagga gatgtataat cccgaggtgt ttggcattat gtattatact aacgctgtgc
     7321 aggtgcaatg atagctatga agccgttgct aactctggaa tgcagatatc gcataaaaaa
     7381 aataataaag ctcagtagcg gcgtcatctt tttggcgata gttatctata tagtgtttcc
     7441 ctataaaaga gattaccttg cgtcatcaaa taaactcccg gttgttatct atgacaggaa
     7501 tgggacggtc cttatggagg tagcaaaagg caaaagtggt ttgtgccagg ctatcagtat
     7561 acaacaactt cccgaggatt ttatttcatt actgttattt tccgaggaca ggaattttta
     7621 ccaccattgg ggtatatcgt taaagagcat tgcacgggca ctgtatcaaa atggcaaagc
     7681 tatgcgcatt gtttccgggg gatcaaccat cacccagcag gttgtaaaat caaaaaaaga
     7741 gatactgcgc aatacggtgt tgacaaaaat gctggagttt attgaggcgg tacgcatgga
     7801 gcttcatttt agcaaggatg agattctgca ggggtacatc aatgaggtgt atttagggaa
     7861 taacatctat ggctttgaaa aggcagccca gatatatttt ggtaagcata taaatgaatg
     7921 caatctgctg gaggcaggtt ttttaattgc aatagtaaaa gcaccggaac gatataatcc
     7981 atatcaaaat cctgaaacag tggttcaaag tgcaaagagg cttttgcgca atgcctgtca
     8041 catgggctat gtaaaaattt ctccctttga actggaggtt tatactaata gcaggattgc
     8101 attgcagtat aatgaaaaga aggttattgc tccattattc tgcctgtatg cattatcgca
     8161 ggctcgtaga ttatttccgg ataaggatat cacgcatatc tataccacgc tggatgcaac
     8221 catatatagc aatgtattaa ccgtgatgca aaattcaatg agcatgctga aggataacaa
     8281 tgcgctgcat gctgcgctgg tgatgattga caacacaacc atggagattg ttgccatgat
     8341 tggaagtgtt gatttttttg actataaaaa agggcaggtt gatgctactc ttataaagcg
     8401 tcaggctgca tccaccatga agccttttgc gtatgctctg gcacttgaaa caggtatgtt
     8461 ccatacatca agtattgtgc cggatgtata tacgcaattc ccctctaagg ttggaaacta
     8521 tataccaaaa aacttcagtg attcctacca tggtccggta cgatttgcaa aggcgctggg
     8581 ttgttcatat aatatacctg ctgtatatgt agtaagtaag attgggcttg tttcttatta
     8641 taatctttta aaaaagatag gttttgattc aataacacgc tcgccatcgt actatggact
     8701 gggtttggtt ctgggcaatg ctgatgtcag cctgctggaa cttgcaaatg cctatacagt
     8761 ttttccacgg cagggattat tttcaaaaac tatcgctata aaaaaaatac gagatgcgca
     8821 tggcaaggaa tatacagtgc agtctgctgg cagcgcaagg gttttgaagc aacaaacatg
     8881 ctatttgatt tcgcatatat tgtccgaata taaatataaa gttgatgcct ttggattgtg
     8941 ctcatccatt aattttccct ttccctttgc agtgaagact ggaacatcaa aagatttcaa
     9001 agataatttt attgcaggat acaacaagca gtataccttt gcggtctggg ttggaaatct
     9061 atataatgag tcactgtgtg atcttccggc ggtatcgggg gctggcattg ttttaaagga
     9121 tgtactgctg tatctatgga atgcagggta tccatttgaa aggtttacca tgcctgaaaa
     9181 tataagaaag gtaaaaatat gtacactgtc cggcatggtt gccgatacct attgtcatag
     9241 tactgattat gagctatatt ccggcaataa ccagccttgc aataaatgta actggcacac
     9301 aaaaaatgca acaattgtac catcacagtt taaagaatgg gcaaggaata aaaattataa
     9361 tgttgctcat gacgcttctg tgcaaattgt ttttcccggg aatggtgtgg tgtttaaaat
     9421 agaaaaaaca gtccgaaaaa atttacaggc tataccattg aaggcaactg ctaatagcaa
     9481 tattcaatgg tatgttgata atcggtatat aggggaaggc agtgaactta tatggcagtt
     9541 atcggcaggg catcatgtta ttagcataca ggctggcaat cagcgcgatg ctgtcaacat
     9601 caccgttctg gaataatgtt catgaattca atatgtcagt agtattgtaa acaaatacaa
     9661 tgcgatatca ttggctatcg tttaaaaatt gtgtactgga atatactgaa tataaccata
     9721 taaaagaata aaaataattg ccataaaata acatattgtt atacatgtca taaatagaga
     9781 aaaaaaggag tgatacgatg atgcataaac tgttttaccc tgattcgatt gctattgtag
     9841 gtttgtcctc gcgtccaaac aacattccac ggcttaccct tgaaaatacc ttgcgatggg
     9901 gttatagtgg aagaattttt ggggttaatc caaaaagcga cgatgagcag gttgacggta
     9961 tcaggatgta taaatccata ggagccttgc ctgaaattcc tgatctggtg tatgcactgg
    10021 ttccggctaa atttgttcca ggtattgttg aagaatgtgg tcaaaaaggt gtacagtgga
    10081 tggcaatccc cagtggtgga ttttctgagt atagcgaaga agggaaatca cttgcacaga
    10141 aaacggttga tactgcaaaa aaatatggaa ttcgttttgt tgggcccaat gggttgacgg
    10201 tagctaatgt tgaaaatgga ttgtgtttgc catttgttcc tattttacgc ccgcagtttg
    10261 gtgggatgtc gatagtatct caaagcggtg gtgtagcatt gatgatgtgg aatgtcataa
    10321 tggatgaaaa tattggcata gcaaaatttg ccagtattgg taataaatta gatttagatg
    10381 aggtggattt tctggaatat ttaggctctg accccgccac atcaattata tgtatgtacc
    10441 ttgaaagtat tccacgaggt tctgatttta taaaagttgc tgataggata aataaaccaa
    10501 tagttgtata taaatcgaac acaacgcagg ctggaaaaaa agctgcaatg agtcatacag
    10561 ctgctatgag taatgatgag gatattatta acgctgcatt tgaaaaggcc ggtattatcc
    10621 gtatatacaa ttaccatgac ttttttgctg tggccaaagc atttaaattg ccaccaatga
    10681 aggggaagcg tgtgatggtg atgagcccag ctggtggttt tagtgttatg actgcagatt
    10741 tatgtgaaca ggcaggattt aaatttgcaa atcccggcaa agaattttat gaaagcttgc
    10801 agaaattttc caatgctggt gttatacagt tttcaaatcc tcttgatatg ggcgatattt
    10861 atgatcccgc ttttaccgcg catgtacttt attcggttat gcataacaat aatgttgatg
    10921 gtgccatgta cgtaagccag cgtccacaga tgcccgacag gaaaaatgta ttttcacaga
    10981 tgtttttatc cgacctttca aaagaagcat ggggtgccat tgtttcttcg gggaaaccac
    11041 tggcaatttg cctgttaggt atggcaaagt tgatggcaca gataaaacgc tcggtaaact
    11101 tcccaatatt taacagtgcc gaggagatgg tgcgtgcaat gaaattccag atgaaatact
    11161 ggttgcgacg ttctacacct caaatgcatg agtcataccc aatgagcctt gatactgtat
    11221 tacgatggat aaccaatcac catggcgata ttggtgaaga tgccatggaa ctacttgagt
    11281 tcattgatat tcccgtgcca cagtcaggga tagcaaagtc ggaaaaggaa gcggtaacag
    11341 tagcaaaaca acttggctat cctgttgtat gcaaggtcat ttcccgcgat attttacaca
    11401 agtcagatgt tggtggggtg atagttaatg tgaatggaga tgaaggtgta aagcaggctt
    11461 atgcagcaat acaatccagt gtactatcgc ataaacctga tgcacacatt gaaggtatac
    11521 gcatttcaac aatggcacct gatggagtgg atatgtttgt ggggagcaaa tatgatcagt
    11581 catttggaca ggttattatc tttggttttg gtggcatata tgttgaggtg tttaaagaca
    11641 tagcctattg tttatgcccg gctacaaagg aagaaattaa gcaaaagctt acttcattaa
    11701 aatcgtatgc actgcttaaa ggtgcgcgtg gcaaaagtgc actggatgtt gatgcctatg
    11761 ttgacattat atggaaggta tcacatcttt tagccagtgc atcccatatt aaagagctgg
    11821 accttaatcc tgtgcgggtg tttgaacacg gctgtcaggt tttagatgtg aggatgagaa
    11881 tagttcatta aattttaatt tgcaggttgt tgtggcactg ctatacttat ggctatggta
    11941 tatcttgata atgcagcaac cagttttccc aaaccggata cggtaataaa ggctgtaacc
    12001 aattgcttaa caaactactg tgctaatcca ggccgttcag ggcattcact ttccattgaa
    12061 gcagcgcgga aagtatttga aacgcgtgaa actgttgccg aattgtttca tgcaaaagat
    12121 tcacggtatg tcatctttac tgccaatgcc acaatggcaa tcaatctggt ttttcatgca
    12181 cttttgcaaa aaggcgatca tgtcgtcgtc tcatcaatgg aacacaatgc agtgatgcgt
    12241 cctctcagac attgggaatc acagggtatc atcacacttt ctttggcaaa tgccgatggc
    12301 aatggtttcg ttgatgatga cattggtgct ttcatacagc cagccaccag attatgtgtt
    12361 attaatcatg catccaatgt taatggtgtc gtacaggata tagcatctat tgcaggcaga
    12421 ataaaagaaa tcaatcctga tgttgcagtg cttattgatg cttcacagag caccgggctt
    12481 atacctattg atatacaggc aatcaaatgt gattttgtag cgtttaccgg gcataaagca
    12541 ttatatggac ctcaggggat tggtggactt gtaataaata cagcaaagca cttacagccg
    12601 tttatcatgg gtggtaccgg cagtaaatct gaaagccaat tccaaccgca gtttttaccc
    12661 gataaatttg aaagtggtac gcttaatctt ccgggtattg ctggacttaa agcagggatt
    12721 gactatgtaa aatcccgtat ggatgaaata caccataatg aactattgca tataaaaacc
    12781 atagttgaag ggttgtcatc cattaaagat ttgaagcttt tctggagcag atcgttgcat
    12841 aatcagattg gtgttatatc agtagctcat aatcatatac cctgcagcga gattggacaa
    12901 aggctcaatg atgaattttc agtttttgta cgcaccggtt tgcattgtgc accgctggct
    12961 catgaaacac tgggtaccta tcctaatggg acgttacggg tgtcagtagg tttatttacc
    13021 aacaaaagtg atgtcgatgt attttgcaaa gcaatagaaa tcatttcaaa gtgaagaata
    13081 tggactattg ttatttatta ttaccgtcag tacatgatgc aatgaaagct gagaagcttt
    13141 tattacaatc aggttgtgca atacaggtta ttgccacccc gcgggaaata tcgcataact
    13201 gcggggtggt tataaaattt agctgtgctg aaatcaatac ggtactacta tcttgtaagg
    13261 aactgtcggc agaacaaaag atattcaaaa aaaatagtaa taatgtgtat tacgaaatta
    13321 tcccagagta gattctaact tcctgatgct ttcagcaagt atcttatact cttttgaatt
    13381 ttttggaaat gtgctctggt actttttaaa gtactttatc tgttccatct tccactgttt
    13441 catatcaaag ctgttttgaa tcataaataa tcccccttgt acaatactgt actataattg
    13501 tcgtaaaaat gatctattcc attgagaaga aaatttttcg cctgcatttt ttcaacactg
    13561 caaggcattc tatggcttac aatgccttca caataactat cgtacatgca tcatatactg
    13621 ggcgatagtt actatgtatt atgtagtgtt ttgcaaataa gtgcaagcta cagcaggcta
    13681 tatttttttg attaccggtg ttattgatgt actctgatga agatatattt ttttacaata
    13741 aaaaaaagct gctcaaatca gagcagcttt ttttattaaa atgtattcgt aattaccact
    13801 tttcaactaa ttcaaacaat gcagcagcac ccataccgcc gccgatacac attgactcaa
    13861 taccgtattt aataccgttt ttagtcatca tattaacgat ctgtgcagcc aattttgcac
    13921 cggtacatcc taatggatgc cctaaagcta tagctccgcc attaacattg atacggcgtg
    13981 ttttgctgtc aactgcccat tcttttggat cagctatacc aagctgttgt gcacagtaaa
    14041 ctgcctgtga tgcaaatgct tcattgatct caaatatgcc tatgtcttta atatccagtc
    14101 caacaagctt taagagtttt ggaatggcat aagctggtcc aacacccatt tcatctgctt
    14161 tgcatcctgc aacaacgtaa gctttaactt ttgccagtgg ttttacaccg tatttacggc
    14221 atgcttcttc gctcataaga agtgtagcag cagaaccatc agtggtctga gaagagttgc
    14281 cagcagtaac tgaaccaaat gctgcaaagg ctggttttag ttttccaagc ccttcaattg
    14341 tagtatctga acgaacaccg tcatcaaagt cctgcataaa ttcttctttc ttaatagtgc
    14401 catcaggctg gggaacaaac ttagtagcct ttgttggaac aatttcattg tacagcttgt
    14461 tcttttgagc ttcagcagct ttcatctgtg aatgataggc aaattcatcc tgcatctgac
    14521 gggtaatttt atacctgttg gcaacatttt ctgctgttat tcccattgag atataaacgt
    14581 cgggattctg ctttgcccag tagggatgag ggcgtggcat attgcctccc atggggacta
    14641 tcgacatgga ttctaaacca gcacctatag ctacatcaca ccatccggct tgaatacgca
    14701 ttgttgatat tgctatggct tccagaccag aagcacaaaa tcggttaact gttgcaccac
    14761 atgtatcatc ggggaatcct gccatctgag cagctatacg accaacgttt aacccctgtt
    14821 cagcttcagg gaaagcacaa ccgaccataa tatcatcaac gactttcttg tcaacctttg
    14881 ctcgttcgat tacaccctgt agagcagtga caagcaaatc ttcgggtcgt gtctgagcaa
    14941 aagcaccttt gcctctacgg cacccaggtg ttctgacagc ctgtacgata tatgcatctc
    15001 tcataaaaat tttcctcctg tttgcataga ttttctatga ctaaataaat aatggtttgc
    15061 cagtttgtaa gatatgctca gccatcttct gggtattttc tgtctgccac aattcaacaa
    15121 aggcttctcg ctctaacttc aaccagtgtg cttcatcaat ttccatgcca cttggcacat
    15181 caccacctga cattacccat gcaatcttct ttgctataaa ttccatgtga ggagtaatgt
    15241 acatgcctat tcgcatattg agcatttctg catctaccat accctgagct tcacggccaa
    15301 gaaccgcaaa tttttccttc tttggtggag cgtatccttc atcaaccatt cttagcactt
    15361 ctttcttggc ttcgccaatt aaaagatctc tattgaatac gattcggtct ttagcaccaa
    15421 ggaatccttt cttctgagct tcaaaagctg acatggtaac ctgagcctga gctactgcca
    15481 taaagcatgg caggaaaaga cctgctaaat cggtaatctt tgcagctttt ggaacagact
    15541 taagatattt tctccataag ttcatcattc caagaccacc aggaagcaga cctgcaccaa
    15601 tttcaaccag acccatataa agctcagcat gtgctactat cctgtcagca ttaagacata
    15661 cctcgcaacc gccacctaac gtcatgccat aaggagctgc aacaacgggt attggtgcat
    15721 attttgctgc taactgtgaa gtatgaacat tagcaataaa tttatcaatt tctgaccatt
    15781 tttttgcttt tgcaaggctt cccatataag caaggtcacc accagctgag aatgcacccg
    15841 gcatgccgcc agcctggttg cctataacag caccaacacc attctctttt acataatcaa
    15901 tattagcaag catgaaatct acgatttcac cgttaatagc gttcatcttg ctatggaatt
    15961 caaagcagaa tacaccatca cccagatcaa taagtgatat tgaattagtc ttcttaacct
    16021 ctttgccggc gccttttaag ttaaaaatag atattgcagc ttcactcttc ttaacttctt
    16081 tgtaaccgcc atttactaga tcatagtaat actctttgcc gttttctact ttatagaaag
    16141 aagtagcgcc tgatgccagc atcttcttga cattttcagg aaccttcatc ccttcttttt
    16201 ccattcgttc aacagactct tttacgccaa tagcatccca tgcctggaat ggccctaact
    16261 ctaagttata accccatgtc attgcattgt caatttcaac tattgaatct gctatttcag
    16321 ggatacggtt tgctgcatag atagcatcat acgcagtaag cttccatgca aattgagctg
    16381 ctttgtcttt tccccataca attgctttaa tcttttcagc agcagttggc agcttcttgg
    16441 cagcactgac tgattcaaat tcaggcttct gtgcttcccg gtattccatc ttgacaggat
    16501 caagtaccaa tgaaagcctt ttaccatcag gtgttttttc tttcttataa aaaccctgtt
    16561 tggttttatc gcccaaccat ttgttttcga ccattttatt aaaccatgga gtaaccttgt
    16621 atacttcacg gcgttcatct ttttccagaa gctggtatga attttctgca agatgatgaa
    16681 ttgtgtcgag accaaccata tcagaaagtt taaatattgc tgtctttgga cgacccattg
    16741 caggtcccat gatagcatca gcttcatcaa tggtaatgtt ttctgattct aacagcttca
    16801 tagcggtcat gatttcatgg acgccaattc ggttaccaat aaagtttggt gtatcctttg
    16861 cccaaacaat acctttacca agacgttttt caccaaaatc agcaataaat tcaagaacct
    16921 cttttttggt tttttcgcca gggataagct ctaacagatg catgtaacgc accgggttaa
    16981 agaagtgtgt gcccatgaaa tgttctttaa atgcatcgct acaatttttt gacatctctt
    17041 tgagcggtaa gccggaagta ttagatgtta ctattgcagt tggcttgcga atcttgtcaa
    17101 gtttttgaaa taaatcctgt ttaatcttaa gattttcaac aacaacctca atgattaaat
    17161 cgcattcttt taatttgtca aaatcatctt caagattaca tagactgatt cgcgatgcat
    17221 tgctcttatc cataaatgct gctggtttag ccttcatcaa gccatcaaaa ccagcctgga
    17281 caatgcgata ccgtgccttt ggatccttct tctcctcatc tgttaaatta aatggtacta
    17341 tgtcgaagag gagagtgttt acgcctgtgt cagccaacaa agccgcgata ccaccaccca
    17401 tgataccgga gcctataacg gctgcctttt cgattcttct aaccataaga acctcctact
    17461 aagatgtaat aattaaatga aaatttgcat actcctgcgt aaatacaaac atgtaccaat
    17521 gatggaataa taaaataaat tgtcaatatt ttattattga aaaacactaa taatttaata
    17581 ttttatagta tatgaaattt atgttataaa aataattata aacataagtc atattatgat
    17641 aatataatat ttaccatatt ttgtttacta ttaaataaag aaaatatttc cagtacagct
    17701 ttaaaaaatt gtatcaaatg tccatagcaa agtatgttca taatttgata aaattatcat
    17761 aataaaccat atcaaaataa aagtttcagc tttatactga taaagattac atcataggga
    17821 aatctaaaaa aaatttaaat tagttacaag atgtcagcga tagtgtcatg tcttcaataa
    17881 tggtaaatgg tatatttccg gaattttact atcctttgta ccatacggtt ataatgctgc
    17941 attaaaaata tattttttta agttgacagt aaaattatgt gttattattg atgatacaaa
    18001 ttattccaat gtactatggt tagtctggcg ccgtacccaa gtggctaagg gagaggtctg
    18061 caaaaccttt atgcagcggt tcgaatccgc tcggcgcctc attgcccaag tggtggaaca
    18121 ggtagacaca agggacttaa aatcccttgg acttacaagt ccgtgccggt tcaagtccgg
    18181 ccttgggcaa ttat
//
LOCUS       DAOOJH010000045        17844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3091_length_17844_cov_10.491146, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000045 DAOOJH010000000
VERSION     DAOOJH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17844
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3091_length_17844_cov_10.491146"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1060)
                     /locus_tag="PLJ75_07330"
     CDS             complement(134..1060)
                     /locus_tag="PLJ75_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05065.1"
                     /translation="MLSCTDDQINTIIQEENNYTTDKPLTSVPIPGNNGLLLTSNITE
                     NSVKMQWTFANDDSPSELLQYQVIVSQTLTLFSLQEILQSGLLKEDNWSNQPINQSTI
                     TNLSIGKLYHANVCVRDPDKNIAVYTPTSFTTKGRMYLFSAGTYTGDMIFQKASRVVV
                     IVSARDALNALCKSVLQSAYPDLPQDNVIAFISINNDDSIATIPLHFAGIPLDWPVYS
                     PSGIIIAYNWDDLVDSSINKKLQDAGVCDSFWWSGSLADGTCDALNTCNGWTDGTNSF
                     QGRSGAHNKTEQWLSDNERNCNNQHHLLGLCW"
     gene            1348..1420
                     /locus_tag="PLJ75_07335"
     tRNA            1348..1420
                     /locus_tag="PLJ75_07335"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1381..1383,aa:Lys,seq:ttt)
     gene            1477..1549
                     /locus_tag="PLJ75_07340"
     tRNA            1477..1549
                     /locus_tag="PLJ75_07340"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1511..1513,aa:Glu,seq:ttc)
     gene            1939..3366
                     /locus_tag="PLJ75_07345"
     CDS             1939..3366
                     /locus_tag="PLJ75_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD05066.1"
                     /translation="MKENLALSFYPVSLEQIQEKVERKQIRQKNKINDLFQLDLFYNS
                     KANNTRIIKDASGNYITRVDGEIPVDLSVRNGERFLFISYDQSILTHGIHKYPAKFFP
                     ELPRWFIKRYSKEGDLILDPFAGSGTTNVEALLNKRHSVGIDIDPFSRLLLKVKVTPL
                     NEKELLFAQQYFLEAIKNFHVDSLSQKDLPDFPYRDNWFNKEILYELAYIKKQIEIMD
                     VSEDIKDYYKISFSSIIRSVSNADDNCTRTVVRKKLNKKVYPADALKKFVEAILVGTP
                     KMIEFSKMVPKHIDVILPENMDARDIKYNDDYFDLAITSPPYANAVDYPRAHQLESYW
                     LGLVEGSLTPLKKKHIGTESVSFNDYSQLHVFNIEIIDLKISAIFKKDPRRAFIVYKY
                     LKDMESNLSEVYRVLKKGGRYIIVVGNNKIRGEVFENWKYIMQLAGKTGFTIENYFAS
                     EIIKHFIKVPREERISTDWILILRK"
     gene            3372..3875
                     /locus_tag="PLJ75_07350"
     CDS             3372..3875
                     /locus_tag="PLJ75_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05067.1"
                     /translation="MDSGDIGRQASVEGFAHEHIVVGLLMKKYQNVSLVDLPLSPYDI
                     IIVRKLINGSEDIIRAQVKTSKKSIIFTGGIRGGVDREYKSNVKTYIQSPETSDVIIG
                     LNPLKEGSFDMYFIPTVLIKRFGTKSKSIKSMELFKNNYEMLENCKNEEFIIQRCKDS
                     GFKLYEV"
     gene            3865..5067
                     /locus_tag="PLJ75_07355"
     CDS             3865..5067
                     /locus_tag="PLJ75_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPD05068.1"
                     /translation="MKFNNFITSTIVPLYIVAIILFFAQNIFAVTLKWDIQPGSKLEF
                     VYDANVEYYENGMLRKAYSERNIIDLLCYNKETKGQFVKGTFSVYRKNINDYLYRLEE
                     QYSSDFFIRVNGMYDVPEGKFMPNLRHLPSFPEGDVKEGSVWQAPVNIIFNNFSVPLI
                     LLLEAQYIVLYINNESHEAIINYSILIDKTLKDKPYRDDMPIKIYGQYAGQVTWDFMQ
                     NQPKKSVNAYNLLLLFGRTGELNTIEFKMAIDQTNMAYKHIPDNEKKKQQKEIEKELK
                     HDGVSVDSDTRGMIIRFSDILFEFDKYNLNGEALKVLEKLVTIIKAKYPNNEVLVEGF
                     TDNVGSDDYNMILSEKRAQTVATYLKNSLGHDKISYRGLGKNNPIADNTTAEGRQKNR
                     RVEIIIKM"
     gene            complement(5069..5815)
                     /locus_tag="PLJ75_07360"
     CDS             complement(5069..5815)
                     /locus_tag="PLJ75_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05069.1"
                     /translation="METQTTYQNLIDAIIDIEEDAQYRDPGKAFVQLLSEYFFATETS
                     QNKAIAGFICQLPVPDQITKLPTLLKITPEDIKPFLETESINQSLCARIMLQPAYLSF
                     AYPHHSPSFSKMPPEIKGEIIQSIKQRNQSILTAFEKLQQDIKATKERNIKALIALIL
                     KNVHLKTGMPFTKLSEPVSQLIEKNFNFCNEIFIASNKQISEINDDAKIKNILKSLFI
                     IKKFDELTEMSRVFKSEARRFIRRTQRILQ"
     gene            complement(5874..6869)
                     /gene="tsaD"
                     /locus_tag="PLJ75_07365"
     CDS             complement(5874..6869)
                     /gene="tsaD"
                     /locus_tag="PLJ75_07365"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPD05070.1"
                     /translation="MFNKVLGIGLESSCDETAVAIVADGRKIVSHAIFSQIKLHQEYF
                     GVVPEIASRAHLEVINDCIDHCMKQANLSFHAIDYVAATCRPGLVGSLLIALQSAKAI
                     SYALSIPLIGVHHLEAHLYAPFLEGYGIEFPFIGLLVSGGNTALYLVHDFGKFELLGN
                     TTDDAVGEAFDKVAKFLLLGYPGGPIIETLAQQATVKKQLFPKILADSSESYSFSYSG
                     IKTAVIQYIHEHPDADIAEIAYSFQERALEILARRLFEASRRLKISTLVIAGGVASNK
                     RLREIIFEKKKEYHKIFIPSPILCTDNGAMVAGIGYQYFKKRMFSPLTIDVTARA"
     gene            complement(6859..8226)
                     /locus_tag="PLJ75_07370"
     CDS             complement(6859..8226)
                     /locus_tag="PLJ75_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD05071.1"
                     /translation="MIQWLKKRSFHIIVISFLAGIFIGVNIAFNVSAQEPSFKYLDFF
                     HQVYQLIRDEYVEQADSKNLFYGAIRGMIASLNDPFSRFLDEQDFSQLKEETSGEFIG
                     VGIEISAKNGDIVVVSPIEGTPAMKAGIQPGDIIYKVDNKPIKDKNINDIISIIRGKP
                     HTKVILSIIREGIDDPIDIELERLPIKLQSVNYAIIDNTNIGYLRIKVFSEDTASEVK
                     KAFQYFQKNNIPKCIVDLRWNPGGLLDQAVAISDMFLPKDALIVSTKGRSGKETLQEY
                     RSTTIPLYTGKCIILVNKGSASASEIVSGALKDNGRAKLLGEKTFGKGSVQKFFNLDE
                     DTGITLTIAKYYTPSGVSIHGIGIQPDYTVTMATISREEQKQINILLKEKILQNFIDK
                     HSKYDEATRKEFKDVLQKNNIKLSDDTANFILKSEIYRFAKKPLYDLEFDNQLSEAMK
                     IINVQ"
     gene            complement(8247..8525)
                     /locus_tag="PLJ75_07375"
     CDS             complement(8247..8525)
                     /locus_tag="PLJ75_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05072.1"
                     /translation="MKKIFVPILCIIIMAIATGCLHQQLSEDEFSIIWQEYLKREFVE
                     SFDEEQSTMQRRDILNSVLKDFSINEQSFYSYCRTKHPDKFRLFDIKP"
     gene            complement(8727..9332)
                     /locus_tag="PLJ75_07380"
     CDS             complement(8727..9332)
                     /locus_tag="PLJ75_07380"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPD05073.1"
                     /translation="MLTFQNHDEFLHFCERIKQTLQQRPPRQIHIEKKSLKGSAVLIP
                     LLYKDNEPYLLITRRTNKVATHKGEVSLPGGGIDPVDITPLDTALRETHEEIGIKPED
                     ITVLGEFDHFISIYGFHVYTFAGIIPYPYTLTISHDEIESYTEVPLSLFVEEKYDNVD
                     YYTYEGIPYAIYYYQYNGYTIWGLTARILTDFSRKVIKQII"
     gene            complement(9310..9852)
                     /locus_tag="PLJ75_07385"
     CDS             complement(9310..9852)
                     /locus_tag="PLJ75_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HPD05074.1"
                     /translation="MKSIPLTIPLSTDDIGALKAGMLITLNGILYTARDSAHKIIAEM
                     VQNNEPLPINFNNAGIFYAGPCPTPRGLPVGAIGPTTSYRMDAYTEMMLSLGVKVFIG
                     KGYRNNNIKKLLVQYKAIYCSSFGGAAAYLNQCVISSKPVAFEWLGPEAIVELTVNNF
                     PAVIINDIYGGDLYANLSEP"
     gene            complement(9849..10685)
                     /locus_tag="PLJ75_07390"
     CDS             complement(9849..10685)
                     /locus_tag="PLJ75_07390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPD05075.1"
                     /translation="MRTITTHDIYTEVSKLIVEANFYLPGHVQKALHAMYNRETKPLA
                     RETLSILLKNATIAQQNHIPLCQDCGSVIVFIDMGDEVHIQGNLYECINRAVADTYRA
                     HYLRPSMVQDPLFARTNTGNNTPAWIHVAIVEGSSITITVYIKGGGSENMTSLTMFTP
                     TTDVHTIIEYVVSTVKQAGPNPCPPLFLGIGIGGTADVAMLNAKKAVLRNKPHPMQKY
                     KQLEEEILQRCNETQVGPLGFGGNNTVAAVHIKEAPTHIATLPVALNLNCHSLRYGTA
                     VL"
     gene            complement(10701..10931)
                     /locus_tag="PLJ75_07395"
     CDS             complement(10701..10931)
                     /locus_tag="PLJ75_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPD05076.1"
                     /translation="MCLAIPMTVKRIEGTKAVVESMGVEKIVDIALSPDIKINDKVIV
                     HAGFVIEVLDPEAAREIEATWEEYTTLMENKQ"
     gene            complement(10949..11947)
                     /gene="hypE"
                     /locus_tag="PLJ75_07400"
     CDS             complement(10949..11947)
                     /gene="hypE"
                     /locus_tag="PLJ75_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPD05077.1"
                     /translation="MDIIVPGHGSGGKMMNDLINNTIKSILGSDTIQLDDSAILTVPV
                     HSIAFTTDSYTITPIEFSGGTIGSLAVNGTVNDLAVMGAVPRFISCALILEEGLPIET
                     LQRILISMKEAALKAGVAIVTGDTKIVPRGKGDRIYINTAGIGTVESSVQRRPIVPGD
                     SIIVNGTIGDHGTAIMALRNNLSFSKGLSSDCAALNHLIQDIIKHFPDAIKFMRDATR
                     GGVASILNEITKGASFGAKLYEEALPIRDEVKGVSEILGIDPLYSANEGKVVIVADSI
                     YANDIIAIMKKHPEGKDAAVIGTITSEFPEKAYMQTTIGGNRILPVLIEEQLPRIC"
     gene            complement(11948..13006)
                     /gene="hypD"
                     /locus_tag="PLJ75_07405"
     CDS             complement(11948..13006)
                     /gene="hypD"
                     /locus_tag="PLJ75_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPD05078.1"
                     /translation="MDIKKEAVLLSQYTLQTPVAIMEVCGTHTTEFFRTGVKDIFPQG
                     LRLIDGPGCPVCVTSNDYLDTAIAIGWQYDCIIATFGDMMKVPSSYSSLSKEKSKGLT
                     VEVVYSPMDAVQLAADNPDKKIVFLSIGFETTAPAEAVCVLEAKKRNIKNFFILPGNK
                     LTVPAVKVLLDSGEVNIDGFILPGHVSAIIGKKAWNFIATDYGKPAVVVGFEDHDLIR
                     GTLLLLNLITHANPNVVNEYTRVVRDDGNPKALEILYSVFKKGDAIWRGIGTIPESGL
                     LLNDDHAYFDALQQFTPEVPPPKEHPGCRCGELLRGVIIPTDCPLFGKACNPEHAIGP
                     CMVSSEGPCSAYYKYGRK"
     gene            complement(12996..15269)
                     /gene="hypF"
                     /locus_tag="PLJ75_07410"
     CDS             complement(12996..15269)
                     /gene="hypF"
                     /locus_tag="PLJ75_07410"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPD05079.1"
                     /translation="MVHYKIQVNGIVQGVGFRPFVYALATGNNCKGSVINNTEGVTIH
                     LEGDDQSIQQCIDAIKNSPPPLAKIIDIRITKLPLNGYTAFTIEESQITTCKDTFIPP
                     DVAICNECHDEFFDSTNRRYHYPFTTCTHCGPRFSIIDDIPYDRANTSMRSFPMCSNC
                     LAEYRNPLNRRFHTQPNACSVCGPEYRLCDSSGKTILTQFDAIMEQVKALLKDHIVAI
                     KSVGGYHLACDARSDRCVTLLRERKNRPFKPFAIMVSSIEFLETFCSVTAREKELLQS
                     RERPIVLIKLNKTIFSTHVAPHLTYIGVMLPYTPFQYMLFHNNRESIYIMTSANIADE
                     PIIYKDNDAFERLHTIVDYFVTYNREIIAHTDDSVMYVVDEHPFFVRRSRGYVPAPLF
                     IKKTPLHILATGGDLKNSFALARNNFVIVSQYLGDLSTPWGNDLYRKTIAHFSKIFDF
                     KPALVVSDMHPGYFTTLFAHELEEHGVQTIATQHHHAHIASVCQEHGISQPVIGIAYD
                     GTGYGTDHTLWGSEFLIADTTTFTRAAHFDYFKLPGGEHAINNPWKIGLSLLQCTAID
                     SKNFFTNCSSEQEKSLVEEIIQKNINCPLTCSIGRLFDGVAALLGLAHHISTEAEAAM
                     LLEEEALKAYSDTCVLSIPISNSMPYGINTTWLVQEIVSMMDKGIAPATIAMRFHRAI
                     ADVTVRVALMLREQFKIHSVALSGGVFHNRILLHCIVEGLRKNRFDVYTPLQLPCNDG
                     GIALGQIAIGRDTYNGH"
     gene            15890..16288
                     /locus_tag="PLJ75_07415"
     CDS             15890..16288
                     /locus_tag="PLJ75_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPD05080.1"
                     /translation="MSKSKLCLAGAVAVIFLAGCSQGTTSINPSPTDVVTVTDVNKFI
                     TDTDRQAGINATMNAMDPVKLAHKKHEDAGVKCVICHHKQGNDDRIKQCAVCHKGASG
                     EDTLHNLCINCHAKTKQGPTLCNECHIAKE"
     gene            16315..16779
                     /locus_tag="PLJ75_07420"
     CDS             16315..16779
                     /locus_tag="PLJ75_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPD05081.1"
                     /translation="MKPKRTLILGIGNLLQKDDGVGVHVIQYMQQHNVLLPQHVELLD
                     GGIAGFDLISHMIQFDKIMIVDALNVHDKPGSIYRFAGEHLVSKSPNVSLHELGIAEV
                     LKVLKIQGANPDIEVIGIVPENIKDLDMTLSPSVAESVPRVVDLILESVTVH"
     gene            16804..17808
                     /locus_tag="PLJ75_07425"
     CDS             16804..17808
                     /locus_tag="PLJ75_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HPD05082.1"
                     /translation="MSLNRREFLKVMGGTAAAVAFPGVLIQGCKRALQKASERTNVIW
                     IQAQSCSGCSVSLLNKLEPDIATVITEYISLNYHQTLCGGTGHAAIQVLENAVKTNRK
                     DFVLIVEGSIPTKSDEYCTIGDMDGRIIGAREWIQKLGANAMALVAVGTCSAFGGIPA
                     AKIRANGDNPTGATSLQTLFPDKKVINIPGCPPHPDWMVGTLLHVLLKGMPELDEYSR
                     PLMYFGTTVHEKCERLPDYKRGRFAKHWGEEGCLYLLGCLGMDTGCDIPARKWVGGMN
                     TCTGCGSGCIGCTEPPFPDYGNRGIYKHLMASNNEIESLHPQVAETVLKLKNGGVING
                     "
BASE COUNT         5404 a         3198 c         3591 g         5651 t
ORIGIN      
        1 tatgaccatc aaatgacgtt tgagctatta tcacagcagg caatagcata tacagtaaca
       61 aaaaaacact caaaacatat ttatactcaa taactatcac ccgttgttcc agttttaaaa
      121 ataattaaaa cgcttaccag cataacccta aaagatgatg ctggttatta caatttcttt
      181 cattatcaga gagccattgt tctgttttat tatgtgcccc gcttcttccc tgaaaactat
      241 ttgttccatc agtccatcca ttgcatgtat tcaatgcatc acacgtgcca tcagctaaac
      301 tgcctgacca ccagaaacta tcgcacacac cagcatcctg taacttttta ttgatagaac
      361 tatcgacaag atcatcccag ttatatgcta taatgattcc ggatggggaa tataccggcc
      421 aatctaaagg aatacctgca aaatgtagag ggatggtagc aatggaatca tcattattaa
      481 tgctgatgaa cgctataaca ttatcctgcg ggaggtccgg atatgctgat tgcaaaacac
      541 tcttgcataa tgcattgagg gcatcacggg ctgatactat aacaacaact cgcgatgctt
      601 tttgaaaaat catgtcaccg gtatatgtcc ccgcagagaa tagatacatt cttccttttg
      661 tggtaaatga ggttggggtg taaaccgcaa tgtttttgtc aggatctctg acacagacat
      721 tggcatggta tagcttcccg atggatagat tggtgatggt actttgattt ataggctgat
      781 tggaccaatt atcctcttta agcagtccag actgaagtat ttcttgcaat gaaaaaagag
      841 taagtgtttg acttacaata acctgatatt ggagtaattc agatggagaa tcatcatttg
      901 cgaatgtcca ttgcattttt acagaatttt ctgttatatt tgatgttaac agcaagccat
      961 tgttgccggg gataggaact gatgttaaag gtttatcggt ggtataatta ttttcttcct
     1021 ggattatagt attgatttga tcatctgtgc atgacagcat agtgagactt gatacaatgg
     1081 caattcctat gagaacaact ttgcatgtat attctaattg atataccata attattattt
     1141 aatgagtgca ctattaaatt tgcaataaat gtctctttac actatcagca taataaaagt
     1201 gcaggaaaat tgcaactttt ttatgaaaat attataaaat ggagtaattt ttgagcatca
     1261 gcaatgattt ttacattttt tatttgacac caaaaagcaa ttaattatac aaggttcatc
     1321 caattgttac aataagaatt atggtgcgag tcgctagctc agtcggtaga gcaactgcct
     1381 tttaagcagt gggtccggag ttcgattctc cggcgactca ataaaataat attgacagtt
     1441 tcaatgttat ttttttaagt agtctcatta ttacatgtcc ccgtcgtcta ggggtctagg
     1501 acaccgggtt ttcatcccgg caacaggggt tcgaatcccc tcggggatgc attgaaaata
     1561 aaaaagggta cgatgtcaat aaagtgccct tttttatttt tgtaaattca aggaattttt
     1621 ttatctatac tataaagatt atgttacttc tttgaaaaga gaataacacc caccatcatt
     1681 ccgggcttga ttcggaatcc cagtagataa cattagattc tgaaacaagt tcaggataac
     1741 attctcgctt cattgtatcg tatcatggtg gttatagtat ggttaatgtt aacttaccgg
     1801 gtattcattt atgacaggtg ctctataaaa ttttataatg aattatttta ttacttgcaa
     1861 ttatttatat gttctcttta catatgagat atatttcaaa actaataaat atacactact
     1921 gtataaaaag gaatgtttat gaaggaaaat cttgcattat ccttttatcc ggtatctctt
     1981 gaacaaattc aagaaaaagt agagcgaaag caaataaggc aaaaaaataa aattaatgat
     2041 ttattccaac tcgatttatt ctataacagt aaagcaaata acactagaat tataaaagat
     2101 gcatctggca attatataac acgggtagac ggtgagatac ctgtagattt gtctgtcaga
     2161 aatggcgaga ggtttttatt tataagttat gatcagagta ttttaactca tggtatacat
     2221 aaataccctg caaaattttt ccctgaattg ccaagatggt ttattaagcg atattcaaaa
     2281 gaaggggatt taatccttga tccttttgct ggtagtggca ctactaatgt tgaggcatta
     2341 ttaaataaac gccattctgt tggaattgac attgatcctt tctcaagact tcttttaaaa
     2401 gttaaagtta caccattaaa tgaaaaggaa ctattatttg ctcaacaata ttttttagaa
     2461 gctataaaaa attttcatgt tgatagttta tctcaaaaag atttacctga ttttccttat
     2521 cgagacaatt ggtttaataa agaaatttta tatgaacttg catatattaa aaagcaaatt
     2581 gaaataatgg atgtttctga ggatattaaa gattattata aaatttcttt ttcatcaata
     2641 atccgatccg tttccaatgc tgatgataat tgtacaagaa cagtcgttag aaaaaagctt
     2701 aataaaaaag tatatcctgc agatgcattg aaaaaatttg ttgaagcgat attagtcggg
     2761 acacctaaaa tgatcgaatt ttcaaagatg gtgccaaagc acatagatgt tatattacct
     2821 gaaaatatgg atgcacgcga tatcaaatat aatgatgatt attttgattt agcaattacc
     2881 tctccacctt atgcaaatgc agttgactac ccaagagctc atcaattgga aagttactgg
     2941 ttaggtttag tcgaaggttc tttgactcct cttaagaaaa aacatattgg gaccgaaagc
     3001 gtatctttta atgactatag tcagttacat gtatttaata tcgaaattat tgatttaaaa
     3061 atttcagcaa ttttcaaaaa ggatccccgc agagcattta ttgtatataa atatctaaaa
     3121 gatatggaaa gtaatctttc agaagtctat agggtactta aaaaaggagg aagatacatt
     3181 atagtggttg gtaataataa aatacgcgga gaagtttttg aaaattggaa atatattatg
     3241 caattagcgg gaaagactgg ttttactatt gaaaattatt ttgcttcaga aataattaaa
     3301 cattttataa aagtaccaag ggaagaaaga ataagcactg actggatatt gatattgagg
     3361 aaataattag catggatagt ggtgatatag gacggcaagc ttctgttgaa ggttttgcac
     3421 atgaacatat agttgttggc ttgttgatga aaaaatatca aaatgtatca ttggttgatt
     3481 tgccactatc cccttatgat attattattg tcagaaaact catcaatgga tctgaagata
     3541 taattagagc acaggttaaa acttcaaaaa aaagtataat ttttaccggg ggaataagag
     3601 gaggtgttga tcgggaatat aaatcaaatg taaaaacata tattcaatca cctgaaacat
     3661 cagacgtaat tattgggtta aatcctttaa aagaaggttc ttttgatatg tattttattc
     3721 caacagttct cataaagaga tttggtacta aaagtaaatc aattaaatca atggaattat
     3781 ttaaaaacaa ttatgaaatg cttgaaaatt gtaaaaatga agaatttatt attcagcgat
     3841 gtaaagattc agggtttaaa ttatatgaag tttaataatt ttattacttc tactatagta
     3901 ccactatata ttgttgctat tatattattt tttgcacaaa atatctttgc ggtaaccttg
     3961 aaatgggata tacagcccgg gtcaaaatta gaatttgtat atgatgccaa tgtggaatac
     4021 tatgaaaatg gtatgctacg gaaggcatac agtgaacgca atattattga tcttttatgc
     4081 tataataaag aaacaaaagg tcagtttgtt aaggggacat tttcggtata tagaaaaaat
     4141 attaatgact atctctaccg tttggaagag cagtatagct ctgatttttt tatacgtgtt
     4201 aatggcatgt atgatgttcc agaagggaaa tttatgccca atttacggca tttgccttct
     4261 ttcccggagg gtgatgtaaa agaagggagt gtgtggcaag caccggttaa tattatattt
     4321 aataattttt cagttccgct tattctgttg cttgaagctc aatacattgt gctgtatata
     4381 aataatgaaa gtcacgaagc aattatcaat tattcaattt taattgataa aaccttaaaa
     4441 gacaaaccat accgtgatga tatgccaata aaaatatatg gtcagtatgc aggtcaggtg
     4501 acctgggatt ttatgcaaaa ccagcctaag aaatctgtta atgcctataa tcttttgtta
     4561 ctttttggtc gtactggtga gttaaatact attgagttta aaatggcaat tgatcaaacc
     4621 aatatggcat ataaacatat tccagacaat gaaaagaaaa aacaacaaaa agaaattgaa
     4681 aaggaattaa aacacgatgg cgtttccgtt gattctgaca cgcgcgggat gattatacga
     4741 ttttctgata tattgtttga attcgataaa tataatctta atggtgaagc gttaaaggta
     4801 cttgagaaat tggtgacaat cataaaggct aaatacccca ataatgaagt tctggtggaa
     4861 ggttttaccg ataatgtcgg tagtgatgac tacaatatga ttctttccga aaaacgtgca
     4921 caaacggttg cgacatatct taaaaatagt ctgggacatg acaagatatc ataccgggga
     4981 ttgggtaaaa ataatcctat cgctgataat accactgccg agggcagaca aaaaaacaga
     5041 agggttgaga ttattattaa gatgtaaatt attgtaaaat tctttgtgtt cttctaataa
     5101 agcgcctggc ttcggattta aataccctgg acatctctgt taattcgtca aactttttga
     5161 taataaacag ggatttcaat atatttttta tctttgcatc atcgtttatt tcagatatct
     5221 gtttgttgga agcaataaaa atttcattac agaagttaaa gtttttttca atgagttgcg
     5281 atacaggttc agatagtttt gtaaatggca ttcctgtttt aagatgaaca ttttttaata
     5341 ttaatgctat aagagctttg atgtttcgtt cttttgtagc ttttatatcc tgctgcaact
     5401 tttcaaaagc agtaagtata gattgatttc tttgtttgat tgattgaata atctcgcctt
     5461 ttatctctgg aggcatttta gaaaatgatg ggctatggtg aggatatgca aaactaagat
     5521 atgcaggttg cagcataatc cgtgcgcata gtgattgatt gatactttct gtttcaagaa
     5581 aaggttttat atcctcaggg gttattttta gtaaggttgg cagctttgtg atttgatctg
     5641 gaacaggcaa ctggcatata aagccagcaa ttgctttatt ctgggatgtc tcggttgcaa
     5701 aaaaatattc tgataaaagt tgtacaaagg ctttgccagg atcacggtac tgagcatctt
     5761 cttcgatgtc tatgattgca tcaataagat tttgatatgt tgtttgtgtt tccatagtgt
     5821 tgttaatata tgccaatata gtataatgaa actaagaaat aatcaatatt tttttaagct
     5881 cgtgccgtaa catcaatggt taaagggcta aacatccttt ttttgaaata ctggtagcct
     5941 atacctgcaa ccatagcacc attatcggta cacaaaatag gtgatgggat aaaaatttta
     6001 tgatattctt tctttttttc aaaaataatt tctctaaggc gtttgttact ggcaacacct
     6061 ccggctataa ccagtgtaga tatctttaat cgtcgggatg cttcaaagag tctgcgtgcc
     6121 agaatttcca gggcgcgttc ctggaagcta taggcaatct ctgcaatgtc agcatcagga
     6181 tgttcatgta tgtactgtat aacagcggtt ttaatgccgc tgtatgaaaa tgaatatgat
     6241 tcggaactat ctgccagtat cttggggaat aattgttttt taacggtagc ctgttgggct
     6301 aatgtttcaa taatggggcc accggggtat cccagtaata aaaattttgc aaccttatcg
     6361 aatgcttcac ctaccgcatc atcggtggta ttgcctaaaa gctcaaattt gccaaagtca
     6421 tgtaccagat acaatgctgt attccctcct gaaaccagca accctataaa tgggaactca
     6481 atgccatatc cttcaaggaa tggtgcatac aggtgagctt cgagatgatg aaccccaatc
     6541 agtgggatgg aaagtgcata gcttatagcc ttcgcagatt ggagtgcaat aagcaaagaa
     6601 ccaaccagtc caggtcggca ggttgcagcc acataatcaa tagcatggaa tgataggttt
     6661 gcctgtttca tgcaatggtc aatacaatcg ttgattacct caagatgagc acgtgaggca
     6721 atctcaggca caacaccaaa atattcttga tgaagtttta tctggctgaa aatagcatgg
     6781 gatacaatct tccttccatc agcaactatc gcaacagccg tctcatcgca tgaactttca
     6841 agcccaattc caagtacttt attgaacatt aataattttc atagcctcag ataattgatt
     6901 atcaaattca agatcataca gcggcttttt tgcaaaccgg tagatttctg attttaatat
     6961 aaagtttgct gtatcatcag acagctttat attgtttttt tgcagaacat ctttaaattc
     7021 ttttctggtt gcttcatcat attttgaatg tttatcaata aaattttgta aaatcttttc
     7081 tttcagtaaa atatttatct gcttctgctc ttctctggag atagttgcca tggtaacggt
     7141 gtaatcgggc tgtattccta tgccgtgtat ggaaacacct gacggcgtgt agtactttgc
     7201 gatagtaagt gttataccgg tgtcttcgtc taaattaaag aatttctgaa cagagccttt
     7261 cccaaatgtt ttttcgccca atagctttgc ccgcccattg tcctttaaag cacctgaaac
     7321 aatttcagaa gctgaagcac ttcctttatt gaccagtata atacatttgc cagtatacag
     7381 aggtattgtt gtggaacggt attcctgcaa tgtttccttg ccactacgcc cttttgttga
     7441 tactatgaga gcatcctttg gcaaaaacat atcgcttatt gcaacagcct gatccagaag
     7501 tcctcccgga ttccatcgca aatcaacaat acattttggt atgttgttct tctggaaata
     7561 ttgaaatgct ttcttaactt cggatgcggt atcctcgcta aagaccttta tcctgagata
     7621 cccaatatta gtattatcaa taattgcgta attaactgac tgtaacttaa tgggaagacg
     7681 ttccagctca atatcaatag gatcatcaat accttcacgg ataattgaca gtataacttt
     7741 tgtatgaggt ttaccccgta ttattgatat tatatcatta atatttttat ctttaattgg
     7801 cttgttatca accttgtaaa taatatctcc tggttgaatt cctgccttca ttgccggggt
     7861 cccttcaatt ggtgaaacga caacaatatc gccgttttta gcagatattt caatccccac
     7921 accaatgaac tcaccacttg tttcctcttt caactgactg aaatcctgct cgtctaaaaa
     7981 tcgtgaaaaa ggatcattta acgaagcaat catcccccgg atagctccat aaaaaagatt
     8041 tttagagtca gcttgctcaa catattcatc acggataagc tgatatacct gatgaaaaaa
     8101 atctaaatat ttaaatgagg gctcctgtgc tgatacattg aatgcgatat tcacaccaat
     8161 gaatattcct gctaaaaatg aaatgacaat tatatgaaat gacctttttt ttaaccactg
     8221 tatcatatat tcctcacttt acttatttat ggtttgatat caaaaagcct gaatttgtcc
     8281 gggtgttttg tgcgacagta actgtaaaat gactgctcat tgattgaaaa atccttcaat
     8341 acagagttta aaatatctct tcgttgcatg gttgattgtt cttcatcaaa gctttcaaca
     8401 aattcccgtt ttaaatattc ctgccagatt atggaaaatt catcttcaga aagttgctga
     8461 tgcaaacacc ctgttgcaat agccatgata atgatacaca gtataggtac aaaaatcttt
     8521 ttcattgtta caatcctgac aattttttat aaacattcat taatacagca caccaaaaac
     8581 tatactgtat gcaaccattg taagcacaaa aaaatagtag taaagtagct tcattttagt
     8641 tgcttaccct atgaaagatg tggctatgat gttttttcat tttcaatttt caatagtagt
     8701 atgatggtaa aaaattgtaa agatatttat ataatttgtt taataacttt acgtgaaaag
     8761 tctgttaaaa tacgtgctgt taatccccag attgtataac cattgtactg ataataataa
     8821 atggcatagg gaatgccctc ataggtataa tagtcaacat tgtcatattt ttcttctaca
     8881 aaaagggata acggtacctc ggtataggat tctatctcat catgactgat ggtaagtgtg
     8941 tagggataag gaattattcc agcaaacgtg tatacatgaa atccataaat ggagataaag
     9001 tggtcaaatt cgcccagcac cgttatatct tcaggcttta tacctatctc ttcatgtgtt
     9061 tcacgcaatg cagtgtcaag cggtgttata tcaacagggt caatacctcc cccgggtaac
     9121 gacacttcgc ctttatgagt ggcaacctta tttgttcgtc gggtaataag caggtatggt
     9181 tcattatcct tatagagcag aggaatgagt accgctgaac ccttcaaact ttttttttca
     9241 atatgaatct gacggggagg tctttgttgc aatgtttgtt taatcctttc acaaaaatgc
     9301 aaaaattcat catggttctg aaaggttagc ataaaggtca ccaccatata tatcattgat
     9361 aataacagcc gggaaattat ttactgtaag ttcaactata gcttcgggcc ctaaccattc
     9421 aaatgcaaca ggttttgagc tgataacgca ctggttaaga taggcagcag ctccgccaaa
     9481 gcttgagcag taaatagctt tgtattgtac cagaagtttt tttatattgt tattgcggta
     9541 acccttgcca atgaatacct ttacccctaa cgaaagcatc atctcagtat aggcatccat
     9601 acggtatgat gttgtgggtc caatagcgcc aactggcaac cctcttggtg tggggcatgg
     9661 tcctgcatag aagataccag cattattaaa attaatgggc aatggttcat tgttttgtac
     9721 catttctgca attattttat gggcgctgtc gcgtgctgtg tatagaatcc cgttgagtgt
     9781 gatgagcatc cctgctttta atgcaccgat atcatcggtg gataatggaa tggtcaatgg
     9841 aatagatttc atagtactgc cgtcccgtaa cgcaacgaat ggcagtttaa atttaatgca
     9901 acaggcaatg ttgcaatatg ggttggtgct tctttaatgt gtaccgcagc cacagtgtta
     9961 ttacctccaa agcctaatgg tcccacctgc gtttcattac atcgctgtaa tatttcttct
    10021 tcaagctgtt tgtatttttg cattgggtgc ggtttattgc gtaatactgc ctttttagca
    10081 ttgagcattg ccacatcagc agtgcctcct atgcctattc ccagaaatag tggcgggcag
    10141 ggatttggcc ctgcttgctt taccgtgctg accacatatt caataattgt atgtacatca
    10201 gttgttggag taaacattgt gagtgaggtc atgttctcgg aaccaccgcc tttaatatac
    10261 acggttatgg taattgagct tccttcaact attgccacat gtatccatgc cggggtattg
    10321 ttgccggtgt ttgtccttgc aaacagtgga tcctgaacca ttgatgggcg cagatagtgt
    10381 gcacggtagg tatcggcaac ggcacggttg atacattcat aaaggtttcc ctgtatatgc
    10441 acctcatccc ccatgtcaat aaatactatc accgaaccgc agtcctgaca cagtggtata
    10501 tggttttgtt gtgcaatggt tgcattcttt aaaaggatgg aaagtgtttc ccgtgcaagc
    10561 ggtttggttt cacggttgta catggcatgg agtgcctttt gtacatgccc gggcaggtaa
    10621 aaatttgctt caacaataag ctttgatact tctgtataaa tgtcatgagt tgtaattgtg
    10681 cgcataataa aaacgtgaat ttactgtttg ttttccatca gtgttgtata ctcctcccat
    10741 gttgcttcta tctcgcgggc tgcctctgga tcaaggacct caataacaaa tcccgcatgt
    10801 acaattacct tatcattaat ttttatatca ggtgatagcg ctatatcaac aattttttct
    10861 actcccattg attcaacaac ggcttttgtt ccttctattc gtttaactgt cattggtatt
    10921 gccagacaca tggtatcctc ctttcaggtt aacatattcg tggaagctgt tcctctatta
    10981 ataccggtaa aatcctgttg cctccaattg ttgtttgcat atatgctttt tccggaaatt
    11041 cagaggttat ggtgcctata acagcagcat cttttccctc cgggtgtttt ttcatgattg
    11101 caataatatc attggcatat atactatcgg caacaataac aaccttgcct tcattggcgc
    11161 tgtacaaagg gtctatgccc agtatctcac tcacgccctt tacctcatca cgtatgggga
    11221 gtgcttcttc gtatagttta gcaccaaatg atgctccttt cgtaatttcg ttgagtattg
    11281 atgctacgcc accgcgtgtt gcatcgcgca taaatttaat ggcgtcagga aaatgtttga
    11341 tgatatcctg aatcagatga tttaaagctg cacaatcgga cgatagcccc tttgaaaagc
    11401 ttagattgtt tcgcaaagcc ataatagcag tgccatggtc gccgatagtt ccattaacaa
    11461 taatactatc gccgggtaca ataggtcgcc tttggacaga gctctccacg gtgcctatgc
    11521 cggcagtgtt tatatagata cggtcgcctt tacctctggg aacaatcttt gtatctccgg
    11581 taactatcgc cacgcctgcc ttgagcgccg cttccttcat tgatataagt attcgttgta
    11641 atgtttctat tggcaatcct tcttccagaa tgagtgcaca tgagataaac cgtggtactg
    11701 ctcccataac ggctaaatcg ttgacggtac cgttaacagc aaggctgccg atagttcctc
    11761 cggaaaattc gatgggtgtt atagtgtagc tatcagtggt gaaggctatg gaatgaaccg
    11821 gtacggtaag gatggcagag tcatctaact ggatggtgtc acttccaagg atagatttga
    11881 ttgtgttgtt gatgagatca ttcatcatct tgccgccact accatggccg ggaacaatga
    11941 tatccattta ctttctccca tatttatagt atgctgagca tggtccctcg gatgatacca
    12001 tgcacgggcc aatggcatgc tcgggattac acgctttgcc aaataaagga caatcggttg
    12061 gtataatcac accgcgcaaa agttcgccgc agcggcatcc cggatgctct tttggtggcg
    12121 ggacctcggg ggtaaactgc tgtaatgcgt caaagtatgc atggtcatcg ttgagcagca
    12181 atccactttc agggatagta ccaataccgc gccagatggc atcacctttt ttaaatacgc
    12241 tgtagagtat ttccagtgct tttggattgc catcgtcacg aacaacacgg gtatattcgt
    12301 tcaccacgtt tgggttggcg tgcgtaatta aattgagcaa tagcagtgtt ccgcggatta
    12361 gatcatgatc ttcaaaacct acaacaaccg ctggtttgcc atagtccgtt gctatgaaat
    12421 tccatgcttt tttgccaatg atagcgctga catgcccggg caatataaat ccatcaatat
    12481 ttacctcgcc agagtcgagc aacaccttaa ctgccggcac ggtaagcttg ttaccgggta
    12541 aaatgaaaaa gtttttaata ttgcgttttt ttgcttcaag tacacaaact gcctcggctg
    12601 gcgctgttgt ttcaaagcct atacttaaaa aaacaatttt tttatcagga ttatcggcag
    12661 ctaactgcac cgcatccatt ggtgaataca caacttctac cgtcagccct tttgattttt
    12721 cttttgacag tgagctgtac gatgagggta ccttcatcat atccccaaag gtagcaatga
    12781 tgcaatcgta ctgccagcca atagcaatgg ctgtatcgag atagtcattg gatgtaacac
    12841 aaacagggca tcccggccca tcaataaggc gcaatccctg cggaaatata tcctttacgc
    12901 cggttctaaa aaattcagtg gtatgggtgc cgcacacctc cataattgca acgggtgttt
    12961 gcaatgtgta ttgtgataag agcaccgctt cttttttaat gtccattata ggtatccctg
    13021 ccaatagcta tctgtcctaa tgcaatgccg ccatcgttgc acggtaattg taatggtgtg
    13081 tatacgtcaa acctattttt acgcagtccc tcaacaatac agtgcaataa aatacgatta
    13141 tggaaaacgc cgccggaaag tgcaacgcta tgtattttga attgttctcg caacatgaga
    13201 gctacccgca cggtaacatc ggcaattgcc cggtgaaagc gcatggcgat agttgccggc
    13261 gctattccct tatccatcat gctgacaatc tcctgaacaa gccatgttgt gtttatgcca
    13321 tagggcatgg aattgcttat tggtatggac aatacacagg tatcagaata tgcttttaac
    13381 gcctcttctt ccagaagcat tgccgcctca gcttccgtac tgatatggtg tgctaatccc
    13441 aacagtgctg ctacaccatc aaaaagcctg ccaatactgc aggtaagcgg gcagttgata
    13501 tttttttgta taatctcttc aacaagggat ttttcttgtt cactgctaca attggtaaaa
    13561 aaatttttac tgtctattgc cgtacattgc aacagtgata gcccaatctt ccatggattg
    13621 ttgatggcat gctctccgcc aggcagttta aagtaatcaa agtgcgcagc gcgagtaaag
    13681 gttgtagtat cagcaattaa aaactcgctc ccccacaggg tatggtcggt gccatagcct
    13741 gtgccatcgt acgcgatacc aatgacgggc tgggatatcc catgctcctg gcataccgag
    13801 gctatatgtg catgatgatg ctgtgtggca atggtttgta ctccatgctc ctccagttca
    13861 tgggcaaata atgtggtaaa atagccgggg tgcatatcgc tgactaccag tgcaggtttg
    13921 aagtcaaaga ttttactaaa gtgggcgata gtttttcggt aaaggtcatt gccccatgga
    13981 gtggacagat caccaaggta ctggctcaca ataacaaaat tattccgcgc cagtgcaaaa
    14041 ctgtttttta gatctccacc ggttgcaagt atatgcagcg gagttttttt tatgaagaga
    14101 ggcgcgggaa catagccgcg agagcgacgg acaaagaagg gatgctcgtc aaccacatac
    14161 ataaccgagt catcggtgtg agctattatt tcacggttgt aggttacaaa ataatccaca
    14221 atagtatgga gacgctcaaa cgcatcatta tctttataga taataggttc atctgcaatg
    14281 tttgcgcttg tcatgatata gatgctttct ctattattgt gaaaaagcat atactggaaa
    14341 ggggtgtagg gaagcataac gccaatatag gttaaatgcg gtgcaacatg agtgctgaag
    14401 atggttttgt taagtttaat cagcactatg gggcgttccc tgctttgcaa aagctccttt
    14461 tcccgggcgg tgacagagca aaatgtttct aaaaattcta tggaggaaac catgatggca
    14521 aagggtttga atggtcggtt tttacgttct cgcaacagcg ttacacatcg gtcactgcgg
    14581 gcatcacatg ctaaatgata gccgccaaca ctttttatgg caacgatgtg gtctttgagc
    14641 aatgccttta cctgttccat aatggcgtca aattgtgtca ggatggtttt accggaacta
    14701 tcgcatagcc tgtactcagg cccacaaaca ctgcaggcat tgggctgcgt atgaaagcgg
    14761 cggtttaacg gattgcggta ttctgcaaga cagttactgc acatggggaa ggagcgcatg
    14821 gatgtgtttg ccctgtcata ggggatgtca tcaataatgc tgaagcgcgg tccgcaatgg
    14881 gtgcatgttg taaacggata atgatagcgc ctgttggtac tatcaaaaaa ttcatcatgg
    14941 cattcattac agatggcaac atcaggcggg ataaaggtat ctttgcaagt tgttatctgg
    15001 ctttcttcta tggtaaaggc agtatagccg tttaatggaa gctttgttat acgtatatca
    15061 ataattttgg caaggggagg aggtgaattt tttatggcat caatacattg ctgtattgac
    15121 tggtcatcac cttcaaggtg aatggtaacg ccttcggtat tgtttataac actacctttg
    15181 cagttattac cggtagcaag agcatacaca aagggcctga atcccacacc ctgaacaatc
    15241 ccgttgacct gtattttata gtgtaccata gttaaaattg tagcacatgt tacctgttta
    15301 tagggaatgg gcacactttt ttgcaataaa aaaaggcttt gcttttttac attgagggtg
    15361 gcaatacaga tatttaagaa ttttataata ttttaaataa tagttgacat tacatccatt
    15421 ctattatatt acagcatcat tgcaataatg gataggttac tttgctatgt agttagttga
    15481 tgtgcaatgt agtatgggta actttactgc tgaaactgtt cgttattttt ttagactgaa
    15541 agcattggat gtttgcacaa taatagtata cagtttcaat taatgttgta tggttgtcat
    15601 gactggtagt tggggcacac attgaatatt tgcatatatt aaattatttg cattaattgt
    15661 ttataatgtg aaaaaattta gattgacaca tatggaaaca ttcattgatg ataaacaaag
    15721 gttgcacata ttgtaaatag gtcattctgg ttaaggatga tgtgttgttt atggtttacg
    15781 taaggtgtgc aataatatac tattttagat taatatatta ttgcaggatt tattattcat
    15841 ttattataaa atttaatgaa gatattacaa tagtttaagg aggtaaaatg tgagtaaaag
    15901 caaactgtgt ttagcaggtg ctgttgcggt tattttttta gcaggatgtt cgcaaggtac
    15961 caccagcatt aatcccagtc caactgatgt cgtaacagtt acggatgtta acaagtttat
    16021 tactgatact gacaggcagg ctggaattaa tgctacaatg aatgccatgg accctgtaaa
    16081 gctggctcat aaaaaacatg aagatgcagg ggtaaaatgt gtcatttgcc atcataaaca
    16141 gggcaatgat gataggatta aacaatgtgc ggtgtgccat aagggagcaa gtggtgagga
    16201 tacattgcat aatctatgta ttaactgtca tgcaaagaca aaacaaggtc ctactctatg
    16261 taatgagtgt cacatagcaa aagaataata aagctcttaa agaaagaatg tgcaatgaaa
    16321 cctaaacgaa cgttaatact gggtataggg aacctgctac aaaaagatga tggtgttggt
    16381 gtgcatgtga ttcagtatat gcagcaacat aatgtattgt tgcctcagca tgttgaattg
    16441 ttagatggtg gtatagctgg atttgatctt atatcgcata tgatccagtt tgataaaatt
    16501 atgattgttg atgcattaaa cgttcacgat aaacctggta gcatatacag gtttgcggga
    16561 gagcatttag tatcaaaatc tcccaatgtt tcattgcatg aattaggcat tgccgaagtg
    16621 cttaaggttt tgaagataca gggggctaat cctgatattg aagtcattgg aatagttccg
    16681 gaaaatatta aggatttaga tatgacatta agcccttcgg ttgcagaatc agttcccaga
    16741 gttgtggacc tgattcttga aagcgttact gttcattaac ctgattaata aggaggatat
    16801 tacatgtcgc taaatagaag agagttttta aaagtaatgg gtggtacagc agccgcagtt
    16861 gctttcccgg gtgtgctcat tcagggttgt aaacgggcat tgcagaaagc ctctgaacga
    16921 acgaatgtta tatggattca ggcacagtcc tgttcaggat gctcggtatc actgctaaac
    16981 aaattagaac cggatattgc taccgtaata actgaataca ttagccttaa ctatcaccag
    17041 acattgtgtg gtggtaccgg tcatgcagca atacaggtat tggaaaatgc ggttaaaacc
    17101 aaccgaaaag attttgtatt gattgtggaa ggatcaatac ctacaaaatc cgatgaatat
    17161 tgcaccattg gagatatgga tggcaggatt attggagcac gggaatggat tcagaaatta
    17221 ggtgctaatg caatggcatt ggttgctgtt ggtacatgct cagcatttgg tggcattcct
    17281 gctgcaaaga tacgggctaa cggtgataat ccaacagggg caacatcatt acaaacatta
    17341 ttccccgata aaaaggtgat taatattcca ggatgcccgc ctcatcctga ctggatggtt
    17401 ggcacactat tgcatgtatt attaaagggg atgcccgaac ttgatgaata tagcaggcct
    17461 cttatgtatt ttggaactac tgttcatgaa aaatgtgaaa ggcttcctga ttataaacga
    17521 ggtcgatttg ccaaacattg gggtgaagaa ggatgtttat acctattggg atgtttgggt
    17581 atggatacag ggtgtgatat acctgcgcgg aaatgggtgg gtggcatgaa cacctgtacc
    17641 ggttgtggat cagggtgcat tggatgtaca gaacctccat tcccggatta tggcaacaga
    17701 ggtatctata agcatctgat ggcaagcaat aatgaaattg aaagcttgca tccacaggtt
    17761 gctgaaactg tactaaaact taagaatgga ggtgtgatca atggctaagg tattacttga
    17821 tccaattacc agaatagaag gaca
//
LOCUS       DAOOJH010000046        17419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3179_length_17419_cov_9.162290, whole genome shotgun sequence.
ACCESSION   DAOOJH010000046 DAOOJH010000000
VERSION     DAOOJH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3179_length_17419_cov_9.162290"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLJ75_07430"
     CDS             complement(<1..257)
                     /locus_tag="PLJ75_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD05083.1"
                     /translation="MIHEKNFLYHLSQIRQKLFFYLEKQMAHHGIHDIAPSYGDILYV
                     LDKKGVMTLQQLGSLTMKDKSTVSSVVTRLERLGYVQKKIS"
     gene            complement(429..2027)
                     /locus_tag="PLJ75_07435"
     CDS             complement(429..2027)
                     /locus_tag="PLJ75_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPD05084.1"
                     /translation="MKVVTALQMQEIDRLTINEYGIPGEVLMAIAGKSVADYIKTYLY
                     ADAIAVLCGVGNNGGDGYVAAYFLSQYIKTDIFVAGATQKLTPSSRIYFELCKKTAIP
                     VYELTDTILQTGIFNNYDIIVDALTGTGFSGTPKGLLLQGIEAINKSCKAVVSIDMPS
                     GLPADGILQSEAIVKAHVTVTMGLPKVNLVTYPGKDYAGHVVVADIGFPEVLLKSNDI
                     KRQLIDDDYIISNYRPQKNYDAHKGTNGHLLCIGGFDGMEGAIMLVSEAAFAAGIGLV
                     TIATTPKARTIIAGKIKEAITKEIELDDYADVFIDEMTHEVLKQKQFIELTERITTIL
                     QQLITSTRCSACAIGPGLGRSIFSAAVVKSAIAIVKERAIPAIIDGDGLFHCAMLHEN
                     FSPDKTCIITPHFKEASRLLNVPVDDIKQDRLSFAEKLAGATGAITVLKGPASIVSDG
                     VHTTINTTGNPALATGGSGDVLTGIIGSLLANRITPLVAASLGVYIHGKAADIYCDDS
                     GYTIMQAGDIIQNIRKAYGCLPWM"
     gene            complement(2113..3174)
                     /locus_tag="PLJ75_07440"
     CDS             complement(2113..3174)
                     /locus_tag="PLJ75_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05085.1"
                     /translation="MIKKSIALLAFCVMTSCGYFRVTDLKSTKVCTIPSGTALHAISI
                     NYNESGLQDMSFGVVLADDKVYVKDNIQRKVLVYDRNGNCKIAIGNVSGIADEKDVKK
                     TQVSFDAIGTMVIDSENNLYVQNRLTSVQRISAAGTQIDMSPSFILVFNKEGNLQYTI
                     GQTGMPTVPFYYIEYLEIDNNDRLFVIARTYDIWSVYRFDKRQRDYYMTFTKTHFVDM
                     VDANTSYTGKIENIKILSSGDGFVLSVAYYHNTRFKYRKIYSYDIKSSKPTEILKISE
                     PKNELFTVIQNKYIYLWNVEDTNDVKFLLYNFDGTIIGNIRCKFPQQVLFSDIGISNE
                     GKLYSYHAYASGLEIYEWE"
     gene            3372..4529
                     /locus_tag="PLJ75_07445"
     CDS             3372..4529
                     /locus_tag="PLJ75_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD05086.1"
                     /translation="MDFNLSEEQQMVKDMCRKFADNELAPKAEHYDKTHEFPWEHVKK
                     LGDMGMMGVVYPEEYNGSGMDYLAYAIAVEEISRGCASTGVIVSAHNSLCLSPIYYFG
                     TDEQKKKVLPKLCTGEWIGCFGITEPNAGSDAAGTKTTAEFKNGKWVLNGTKNFITNG
                     GVASVAVVMAITEKGVGHKGLSMFIVEKGTPGFSVGKTEDKLGICASSTTELVFENCE
                     LPEGNLLGKKGDGFKIAMHTLDGGRVGIAAQALGIAQAALDAAAKYALERVQFNQPIA
                     RQEAIQWMLADMATEIQAARLLTYQAAILIGSGADRRRFSKNAAMAKLFASETSHRVT
                     HKALQIFGGYGYIKDYPVERFYRDSRITEIYEGTSEIQRLVIASNVLNEYQ"
     gene            4535..5491
                     /locus_tag="PLJ75_07450"
     CDS             4535..5491
                     /locus_tag="PLJ75_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05087.1"
                     /translation="MTGAQKAVSLSTMKALKRYTMKNDYTTFLKEIQQTILSFSEGDI
                     SFDEALQRYNAYTEKLKNDNIGKQYEDYIINCLGTHNAYNFSIFSALVAYLPGEPCCK
                     ICSFYGPDTFITTINSMCDKDFFSSPPRFLLDYCVHDKVSQNFSFHSSLIVNSESFSI
                     IFIAITSSSFFSKDSFLQFAAQLKTLMSPLTVTDSMSLDIPNCTLQYIHQTCSKLLPA
                     PLTLYGIHLISPFSTLSHFGLYSLLEVSSIIKNRLIDIFGQYAFALSMNRYLCISTNP
                     DINTRLFTIVYKEISIPAKFYKKTIEKEEEITPTLIEILHFR"
     gene            5507..6004
                     /locus_tag="PLJ75_07455"
     CDS             5507..6004
                     /locus_tag="PLJ75_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HPD05088.1"
                     /translation="MRKKQIVIIGSSDDTVHYDTAYVIGAFIASNSWILITGGRTGVM
                     EAASKGAHDNGGIVVGILPFDTFNDANSYCDIVIPTGIGFARNSINVLSADVVISICG
                     KSGTLSELSYAWQYGKPIISCVFTGGWSQRIAEEPIDDRQNGSTLYAAYSLDDVFSFL
                     YKLIQ"
     gene            complement(6006..8390)
                     /locus_tag="PLJ75_07460"
     CDS             complement(6006..8390)
                     /locus_tag="PLJ75_07460"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPD05089.1"
                     /translation="MHHQENTNLIKTVTIVGAGSWGTALANVIGHNHPEMTVRMWAYE
                     KDVVRTINNNHINTAYLPGIILADSIIAYHDLKESVKQTDAIIIATPSKVVYDICVKL
                     RRHIQPDTYCGYLSKGFCKIGSGIYTMSQTIELALPFLMDTVCAVYGPSHAEEVCKEY
                     HTALHIASKSNSSINFFMNLLSNEYIECIPINDIIGVDCGSTLKNPAAIAAGLISNLP
                     QCGDNLMGALIAQALKEMVQLGKCLGGQEDTITGIAGLGDLVATALSQHSRNRRFGKE
                     IGEQITQKGTTLSLFDRFLLRFKPDDVLGRMGGRVHYLVEGAYAIEPIIELADRYTIT
                     MPIYRSLYEVLLNKRDPWLLIETIKNPAKYEILTRRARIKAKERKKGIERMSGLIFKN
                     IVVERMIKELSVPEKKREVLDQKNEYIQLLHQYKHLKKPKKFLYELSLYNNLNEKNYE
                     EQLKTIIEFYYTSISDRYVYSFSLLMLKAARLFFYIYGLLYRRKIVEFFEERISLSGD
                     IKYLKKIAMTANPVYICNANQIADSIFVVLALIKFITIPLPRFNINSQLLKNSLLRYL
                     FRVSGGYIVDKTRYSSTLYRETLLNYILICVEHGITILYSNDSSAPNDETTDSISQND
                     IIQDVLIERLSILLQQTSEEIAIIPVEVAHKYYNPVGMPVSFLKIFRNVTKVNISRPI
                     TMSHFSASEALAEDTKMMLRLKLRHDTPLYPHYFVAHSLNKNSGIADIRKISDDVDSE
                     LKLRNLKNSYDVSKIVQDGLDFLELNGYVMIKNTLVSVADNNTEAIRYFAGYLL"
     gene            complement(8517..10280)
                     /gene="rpoD"
                     /locus_tag="PLJ75_07465"
     CDS             complement(8517..10280)
                     /gene="rpoD"
                     /locus_tag="PLJ75_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPD05090.1"
                     /translation="MAKGDVLLESIPEVKKLVELGISNGEITYDELNDILPDKILNSD
                     KIDDIFILLNQLGIDVVEEPTRRNDAAPLKKEKTHSKKSSSSSSETGVSDDPIRLYLK
                     EIGKVSLLSGDQEVKLAKRIEEGEILIENAVLDSTVLLNELVKNHSKVKSGKIKLTDI
                     LRINKLYYFSSFDMHDLEKRYENNMSIIIAEDKKIMQSQAKLKKLSEDSKKALELKEK
                     MMASRSSIREALINLQVHENEINKTAQRILSMVARIKETRHFFNNLENSFNKTVKELK
                     QFGRKLEKNEDVKKILKELKIKNKDELLEIVKDIRNNERKIRRIEQEAGSTCDEIMEW
                     GTQIEIGHRKISLAKKELINANLRLVVSIAKKYANRGMHFFDLIQEGNIGLIKAVDKF
                     EYRKGYKFSTYATWWIRQAITRAISDQARTIRIPVHMIEQINKVMRETRLFQQEFGRE
                     PSPDELADRLGWHVSKVKGVKNVAREPISLETPIGEEEDSLLGDFIEDKEVDSPVNTA
                     AYRLLSEQINAVLSTLPAREQKVIRMRFGLDDGYSHTLEEVGYVFKVTRERIRQIEAK
                     ALRRLRHPTRARKLKDYLDYM"
     gene            complement(10391..12160)
                     /gene="dnaG"
                     /locus_tag="PLJ75_07470"
     CDS             complement(10391..12160)
                     /gene="dnaG"
                     /locus_tag="PLJ75_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPD05091.1"
                     /translation="MAIPRDTIEHIRQKAAIEDIIRRYVPTLTKKGKNYVGLCPFHKE
                     TSPSFTVSPDKQMFYCFGCHEGGNVFTFIAKVEHVDFVQSVKIVGEIVGIPVRNEEKQ
                     NDEYTTLLAINAMIAERYRQALMSAAGKRALDYLLHRGVTQQGIDEFQLGYAPDEWQY
                     ATDILRQKNVPLDIAVKTGNINKSVKNTITHYYDRFRNRVIFPIFDQKNEIIGFGGRI
                     IESGEPKYMNSPESLVFQKRNILYGFNKARQHIAELGRAIVVEGYLDVIGVYQAGIQN
                     VVAPLGTALTQEQLQLLSRLSNEVIIVFDADSAGVKASLRSLDVAANLNITIKIAQLP
                     EMDPFDFVNKRGAREFMAVVDSALKPVDFRIERIIAENKGKSQVNVLKQIFGILRTIP
                     FEVERQHYFKKLSSVFQVDEKAIRADYERFTKGKTTNGVIVTDNNTVDFYAKSQRELV
                     LLLCNYPLLIENAIVDCPLQDFTDTVAKNIYQTLIALYEDNESFSLEKMFDFYPEGEE
                     KKLLTQGFSKQFSFEDPHVAYSEIILNMKLFTIDKKINEFAEKIEKDNRENIQYYITE
                     IEVLRREKEKLTSYIYNKGSIKH"
     gene            complement(12184..12633)
                     /locus_tag="PLJ75_07475"
     CDS             complement(12184..12633)
                     /locus_tag="PLJ75_07475"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPD05092.1"
                     /translation="MSILTTIESNLKDAMKSKNELLSSTLRMMKSDILYEKTKSTQEL
                     TDEKIIEVLTRSAKRRKEAIAEYEKAGRDDLARKEKQELEIIMQYLPQQMSADEIASH
                     VDAVIASMGTITNKDTGKVMGQVMKDLKGKADGQIVKQIVAQKLEKL"
     assembly_gap    12737..12836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13005..13268)
                     /locus_tag="PLJ75_07480"
     CDS             complement(13005..13268)
                     /locus_tag="PLJ75_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPD05093.1"
                     /translation="MMANENHSNDEFPREITFKVIFHNTLFLKEMLINICAENTIDAH
                     ITERESKQGKFISYTITALFAHEEVLHAICSHITRLDGFITMF"
     gene            complement(13284..>14051)
                     /locus_tag="PLJ75_07485"
     CDS             complement(13284..>14051)
                     /locus_tag="PLJ75_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPD05094.1"
                     /translation="NSTHYGIAGYYAKMAADNAMIGITGTNARPSVAPTFGVENMLGT
                     NPLTFGIPADENFPFLLDCATSVSQRGKIEVYARLDKPLPPGWVIDNHGNSCTNPHQV
                     LDGLIKGSMALTPLGGIGEDGAGYKGYGYCTVVEILSAALQAGSYLKMLSGFDMQGNK
                     VPYHLGHFFIAIAIEAFTDIASFKSITGDILRQLRNSARMPGQKKIYTAGEKEYLAYL
                     ERKDKGVPVNNEVMKELVAMRDELNITGYELLTLHAS"
     assembly_gap    14052..14151
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14187..14888)
                     /locus_tag="PLJ75_07490"
     CDS             complement(14187..14888)
                     /locus_tag="PLJ75_07490"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HPD05095.1"
                     /translation="MQLKKLVKNLEDIKSKGFIETIRKGPTAVGYLLEKELGLSETNI
                     TIPDIGGRIEIKATRKNTNSLITLFTFNRGVWRMKQKDLINKYGYRDAQGRQSLYATV
                     SAKVNNVQGFFLEIDNDKHLVLLKNAKEDETIAEWSTYVIAGKFMTKLDRLLLILADS
                     KMVDEKEFFHYNEAYILENPTPEKFLEGFNRNELMIDLRMHLKDYGGVRNHGTAFRIS
                     EKNLTDLYTKKRRLI"
     gene            complement(14892..16205)
                     /locus_tag="PLJ75_07495"
     CDS             complement(14892..16205)
                     /locus_tag="PLJ75_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD05096.1"
                     /translation="MNKDKLLDKSINLSCNNCIQKNNTLYEDSWDFKNANTKEYTHCF
                     HSYPAMMIPQVARRIIEIYGENATLLFDPYCGTGTSLVEANLKGINAIGTDLNPLARL
                     IAQAKTTKIDSKVLDKYLYDFTNYLFSKSFKEEDYSTITLPCITNIDIWFSKSVQYKL
                     AIIFNYIIQKIDNVFIQNFFKVAFSETIRETSNTKNGEFKLVRDKKLIKNEDLDVFGI
                     ILGKLSRNKEGLINFIKVCPQNVVSHIYSFNTVLSIPYDLVPQESVDIVVTSPPYGDS
                     RTTVAYGQFSRFANEWLGFHNANKIDNLLMGGRKNNVNNFKFNNDLLNDVITAISNKD
                     NKRAMEVVSFFKDYESSIVNVSATIKKGGVVCYVVGNRTVKNINIPTNKITAQLFEQN
                     NFVHMETIVRNIPNKRMPLMNSPTNIPGQTGQTMKNEYIVICKKI"
     gene            complement(16391..>17419)
                     /locus_tag="PLJ75_07500"
     CDS             complement(16391..>17419)
                     /locus_tag="PLJ75_07500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05097.1"
                     /translation="GVVADRFNRHAIFMITQTMLCIQSLILGLLVISNYITPASIVLL
                     NLAMGCINAFDIPVRQSFLFDLVADKQLLGNAIALNSVVFNSTRLIGPPLAGFLVAQF
                     GEGLCFLINTTTFAGIILALLLMKDIHYSKILHTSNFWENIKEGFVYVKNHYPIKSVL
                     ILLGLMSLIGMPFMVLMPVVAVKILHGDSHTLGFLLGTAGFGALVGALFIASRSSITR
                     FENKIAAGSLLLGSTLVCFSFSRNLYCSLILIFIIGISLIIQMASCNTFIQSMVDDTM
                     RGRVMSLYGMSHIGMAPIGNLIAGAIAEKIGVAQTLLLCGTGCAIVALWFYKNLHKLK
                     KHIVRYVR"
BASE COUNT         5172 a         3475 c         2855 g         5717 t
ORIGIN      
        1 ttacttattt ttttctgtac atatcctaac ctctcaagcc tggttacaac cgatgatacc
       61 gttgatttat ccttcatggt taatgaacct aactgctgta atgtcatcac ccctttttta
      121 tcaagaacat acaaaatatc accatatgac ggagctatat catgaatgcc gtgatgcgcc
      181 atctgttttt ctaaataaaa aaacagcttt tgacgtattt gcgataaatg atataaaaaa
      241 ttcttttcat gtatcattat gatattctca acatcgataa ttagttttgt acaaaactat
      301 tattattttg tcaacatcaa ttatcaattt attgccaata tttttgtgac ttgcttttgg
      361 gcagtgtatg tattttttta cttatcggga aaaaagctat cagcataata ccatgttatt
      421 acacaatctc acatccatgg taaacaccca tatgctttcc ttatattttg gattatatcg
      481 ccagcctgca ttatggtata acctgaatca tcacaatata tatcagcagc tttgccatgt
      541 atatatacac ctaacgatgc agcaacaaga ggggtaatcc tgttagcaag caacgaacca
      601 ataatccctg taagaacatc tccggaacca cccgttgcaa gcgcaggatt cccggtggta
      661 ttaatggtgg tatgcactcc atcagaaaca attgaagctg gaccctttaa tactgttatt
      721 gcgcctgttg cacctgccaa tttttcagca aaggataaac gatcctgttt tatatcgtct
      781 acaggtacat tcaacaatct agatgcctct ttaaaatgtg gtgtaataat acatgttttg
      841 tccggggaaa aattctcatg taacatggcg cagtgaaaca agccgtcgcc gtcaatgatg
      901 gcagggatag cgcgttcttt cactatggca attgctgact taacaacggc tgctgaaaaa
      961 atacttctac ctaaacctgg cccaatggca catgcactgc atcgcgttga agttatcaac
     1021 tgttgtagta ttgtagttat acgctccgtt aactcaataa attgtttttg cttcagtact
     1081 tcatgagtca tttcatcaat aaaaacatca gcataatcat caagctctat ctcttttgtt
     1141 atcgcctctt ttattttacc tgcaatgata gtgcgagctt taggcgtggt tgctatggtc
     1201 acaagcccta tgcctgctgc aaatgcagct tcagacacaa gcattatcgc accctccata
     1261 ccatcaaatc caccaatgca gagcaaatgt ccgtttgtac ctttatgagc atcgtaattt
     1321 ttttgtgggc gatagttact gataatataa tcatcatcaa tgagctgtcg tttgatgtca
     1381 ttagacttga gcaaaacttc tggaaatcca atgtcagcaa caacaacatg acctgcataa
     1441 tcttttcccg gataagtaac cagatttact ttaggcaatc ccatcgttac cgtaacatgc
     1501 gctttgacaa tagcttcgga ttgcagtatc ccatccgctg gcagtccact tggcatatct
     1561 atggacacca ctgctttgca acttttattt atagcttcaa taccctgtag cagcaaacct
     1621 ttgggagtac ccgaaaaacc cgttccggtc aatgcatcaa caataatatc ataattatta
     1681 aaaatacccg tttggagtat tgtatcagta agctcataaa cgggtattgc ggttttttta
     1741 caaagttcaa aatatatacg cgatgatggt gttaactttt gagtagctcc agcaacaaag
     1801 atatccgttt ttatatattg cgataaaaaa tatgctgcaa catacccatc accaccgtta
     1861 ttgccaaccc cgcatagtac agcaatggca tcagcatata aatacgtttt aatataatca
     1921 gcaactgatt tcccggcaat agccatcaac acttcgccag ggattccata ttcattgatg
     1981 gttaacctat caatctcctg catctgcaaa gcggttacta ctttcatgtt caatcacttc
     2041 ccgtatatat acagtataat catacttact accattgtct atgccttcat tgtgctgcgt
     2101 ttaaatagtt ttttattccc attcatatat ttccaaaccg cttgcatagg catggtatga
     2161 atacagtttg ccttcattgc ttataccaat atcagaaaat aacacctgct ggggaaattt
     2221 acacctgatg ttgccaatga ttgttccgtc aaaattgtac aataaaaatt taacatcatt
     2281 ggtatcttct acattccaca gatagatata cttattttga attaccgtaa aaagttcatt
     2341 tttgggttct gaaatcttta aaatttcagt tggctttgag ctttttatat catagctata
     2401 tatttttctg tatttaaacc gtgtattatg ataataggca accgaaagaa caaaaccatc
     2461 cccactactg agtattttta tattttcaat ttttccggta tacgaggtat ttgcatcgac
     2521 catatccaca aaatgtgttt ttgtaaatgt catgtagtaa tcgcgctgcc ttttatcaaa
     2581 ccggtaaacg ctccatatat cataggtcct ggctataaca aaaagcctgt cattgttatc
     2641 tatctcaaga tattcaatat aataaaatgg caccgttggc atccccgttt gaccaatggt
     2701 atattgcaaa ttaccttctt tattaaatac aaggataaat gatggcgaca tgtctatctg
     2761 agttcccgct gcgcttatac gctgcaccga ggtcaatcgg ttttgtacat acagattatt
     2821 ttccgaatca ataaccattg ttccaatggc atcaaatgaa acctgtgtct tctttacatc
     2881 cttttcatct gctataccgg aaacattacc tattgcaatt ttacaattcc cattccggtc
     2941 ataaacaaga accttcctct gaatattatc ctttacgtat actttatcat cagccagtac
     3001 cacaccaaaa ctcatatcct gaagtcctga ttcattataa ttgatgctta ttgcatgcaa
     3061 tgctgtaccg gaaggaatgg tgcatacttt tgttgattta agatcggtaa cacgaaaata
     3121 gccacatgag gtcataacgc aaaaagctaa taatgctatt gattttttta tcataccatt
     3181 gtccttaatt accataacta tcgcccacta taatttttta taaccattta tataaagtag
     3241 caatcttttt ttcaacataa aaaagcatcc cattttacaa cactgttcgt tattgccttg
     3301 acaaaaacat agtttatttt acacaataat aaaaagacat tattatacag atataataag
     3361 gagatcggtt tatggatttt aatctatctg aagaacagca aatggtaaaa gatatgtgca
     3421 gaaaatttgc tgataatgaa cttgccccaa aagctgagca ctatgataaa acacatgaat
     3481 ttccatggga acatgtaaaa aagttaggtg atatggggat gatgggcgtt gtatatccgg
     3541 aagagtacaa tggttccgga atggattacc ttgcctatgc gatagctgtt gaggaaattt
     3601 cacgaggctg tgcgtccacc ggtgttatcg tttctgctca taattcattg tgcctttcgc
     3661 caatttatta ttttggcacc gacgaacaga aaaaaaaggt tttgccaaaa ttatgcacag
     3721 gcgagtggat tggctgcttt ggaataaccg aaccaaatgc aggttctgat gcggctggaa
     3781 caaaaactac tgctgaattt aagaatggta aatgggtctt gaacgggaca aaaaatttta
     3841 ttaccaatgg cggtgttgcc agcgttgctg tagtaatggc aattactgaa aagggtgttg
     3901 gccataaggg attgagtatg tttatagttg aaaaaggtac tccgggcttt tcagtgggca
     3961 aaactgaaga taagcttggc atttgtgctt cttcaaccac tgaacttgtt tttgaaaatt
     4021 gcgaactgcc tgaaggtaac cttttaggga aaaaggggga tggctttaag attgcaatgc
     4081 ataccctgga tggtggacgc gttggtattg ctgctcaggc tttaggtatt gcccaggctg
     4141 ctctggatgc tgcggctaaa tatgctctgg agcgagtaca atttaaccag cctattgccc
     4201 gacaggaggc tattcaatgg atgttagctg acatggctac tgaaattcag gctgctcgcc
     4261 ttttaaccta tcaggctgca atactcattg gaagtggagc agacagacgt agattttcaa
     4321 aaaatgcagc aatggctaaa ctctttgcct ctgaaacatc gcatcgtgta acgcataaag
     4381 ctttgcaaat ttttggcggt tacggttata taaaggacta tccggttgaa cgattttacc
     4441 gcgattcacg aattaccgaa atatatgaag ggacatcaga aattcaacgg cttgttattg
     4501 catcaaatgt tcttaatgaa tatcaataat atcaatgaca ggggcacaga aagctgtgtc
     4561 cctgtcaaca atgaaagctt taaaacggta tacaatgaaa aatgattata ccactttttt
     4621 aaaagaaatt caacagacca tcctttcgtt ttctgaaggt gatatatcat ttgatgaagc
     4681 attgcagcgt tataatgcat ataccgaaaa gctgaaaaat gataacattg gaaaacaata
     4741 tgaagattat atcatcaatt gtcttggcac ccataacgcc tataactttt ctattttttc
     4801 cgctttagtg gcatatttac ccggtgaacc atgctgtaaa atttgttctt tttacggtcc
     4861 cgataccttt atcactacca ttaacagcat gtgcgataaa gatttttttt catcaccacc
     4921 ccgattttta cttgattact gtgtacatga taaagtaagt caaaattttt ctttccatag
     4981 ctcattgata gtgaactctg aaagttttag tattattttt atagcaataa catcatcatc
     5041 ctttttttca aaagattcat ttcttcaatt tgcagctcag cttaaaacgt tgatgagtcc
     5101 attgacagtt accgatagta tgtccttaga tatacccaac tgtacattgc agtacattca
     5161 tcaaacatgc agcaaattac tgcctgctcc acttacattg tatggtatcc atttgatatc
     5221 acccttcagc actctgagcc attttggttt atattcactt ttagaggttt catctataat
     5281 aaaaaacaga ttaattgata tttttggtca atatgcgttt gctctttcta tgaaccgata
     5341 tctgtgtatc agtacaaatc ccgatatcaa cacccgttta tttaccattg tttataaaga
     5401 aatatccatc ccggcaaaat tttataaaaa aaccattgaa aaggaagaag aaattacccc
     5461 aacattgata gaaatccttc attttcgata aataaagagc tttttcatga gaaaaaaaca
     5521 aattgttatc ataggatcat ctgatgatac tgtacattac gatacggcat acgtcattgg
     5581 ggcgtttata gcaagcaata gctggatact tattaccggt ggccgaactg gcgttatgga
     5641 agcagcatca aagggtgcgc atgataacgg tggtattgta gttggtattt tgccatttga
     5701 tacattcaat gatgccaata gttattgtga tatcgtcatt ccaactggaa taggttttgc
     5761 acgtaacagt atcaatgttt tatcagcaga tgttgttatt tcaatttgtg gaaaatcggg
     5821 aacactgagc gaactatcgt atgcatggca atatgggaaa ccaataataa gctgtgtttt
     5881 caccggagga tggagccaaa ggattgcaga agagccaatc gatgacagac aaaacggaag
     5941 tactctctat gcagcatact cacttgatga tgtattttct ttcttatata aactgataca
     6001 gtaagctata gcaaataacc tgcaaaataa cgtattgctt cagtattatt atccgctact
     6061 gataccaggg tgttttttat cattacatag ccgttcaact caagaaagtc taatccatcc
     6121 tggactattt ttgaaacgtc ataagagttt tttaaatttc ttaattttaa ctcgctatca
     6181 acatcatcag atattttcct tatatcagca attccactat tcttgtttaa tgaatgtgct
     6241 acaaaataat ggggatacag cggtgtatca tggcgcaact ttagccttaa catcatctta
     6301 gtatcttcag caagcgcttc gctggctgaa aaatgagaca tggttatggg tcgtgaaata
     6361 tttacctttg ttacattacg gaaaatttta agaaaagaaa ccggcatacc aacagggtta
     6421 taatatttat gggcaacttc aacagggata atagcaatct cctctgatgt ttgttgtaaa
     6481 agaattgata gcctttcaat aagtacatcc tgaataatat cgttttgtga tatggagtca
     6541 gttgtttcgt catttggtgc acttgaatca tttgaataca gtatggtaat accatgttca
     6601 acacagatta aaatgtaatt gagcagtgtc tcacgataca gtgtagaaga ataccgtgtt
     6661 ttatcaacaa tataaccacc actcaccctg aaaaggtagc gcaacaaaga gttttttaat
     6721 aactggctgt taatattaaa tcgcggcaaa ggaatagtta taaattttat gagcgccaga
     6781 acaacaaaaa ttgaatcagc aatctggttg gcgttacaaa tatagaccgg attggctgtc
     6841 attgctattt tttttaaata tttaatatca ccacttaaag aaattctttc ctcaaaaaat
     6901 tctactattt tacgccggta tagcaatcca tatatataaa aaaacagccg tgctgcttta
     6961 agcattaata aagaaaatga atatacatat ctatcgctta tgctggtata ataaaattct
     7021 ataattgttt taagctgctc ttcataattt ttttcgttca gattgttata gagcgatagt
     7081 tcatataaaa attttttagg tttttttaga tgtttatact ggtgcagcaa ttgtatatat
     7141 tcattttttt gatcaagcac ctctcttttt ttttcaggga cagatagttc tttgatcatt
     7201 cgttcaacga caatattttt gaatatgagc ccgctcatcc gctctatgcc tttttttctt
     7261 tcctttgctt taattcgcgc cctgcgggta agaatttcat attttgcggg attttttata
     7321 gtctcaatta aaagccatgg atctctttta tttaacaata cctcatataa cgaacgataa
     7381 ataggcatgg tgatggtata tctatcagcc aattcaataa tgggttcaat agcatatgct
     7441 ccttcaacaa ggtaatgtac ccttccaccc attcttccca gaacatcgtc gggtttaaac
     7501 cgtaaaagaa atctatcgaa caaacttaat gtcgtacctt tttgtgttat ctgctctcct
     7561 atttctttac caaacctcct gttcctgcta tgctggctta atgcagtagc aaccagatca
     7621 cccagacctg ctataccggt tattgtatcc tcctgccctc ctaaacattt acccagttgc
     7681 accatctctt tcagcgcctg tgcaattaga gctcccatga ggttgtcacc acattgtggc
     7741 aggttgctga taagccctgc agcaattgca gcaggatttt tcaaggtgct tccacaatca
     7801 acaccaatga tatcgtttat aggtatacat tctatgtact catttgatag taagttcata
     7861 aaaaaattaa ttgagctatt ggatttgctt gctatgtgta atgcagtatg gtattcttta
     7921 caaacctctt cggcatggct gggtccatat actgcacaaa ctgtatccat taaaaatggc
     7981 aacgcaagtt ctattgtttg cgacattgta tatatcccac tgccaatttt acaaaatcct
     8041 tttgaaagat aaccacaata ggtatcaggc tgtatatgac gtcgtaattt tacacagata
     8101 tcataaacaa cctttgaagg agtggcaatg ataatagcgt cagtttgttt cactgattct
     8161 ttgagatcat gatacgcaat tatagaatca gccagtataa taccgggaag atatgcagtg
     8221 ttgatatgat tattattaat ggtgcgcacc acatcctttt catacgccca catcctgaca
     8281 gtcatttcag ggtgattgtg ccctataaca ttagctaatg ctgtacccca ggaaccagct
     8341 ccaacgatgg taacagtttt aattaaattt gtattttcct gatgatgcat attaattatt
     8401 cacactatac aatgggcatt acaatgattt attagtgtac ctttaattta atagataaaa
     8461 gataccatct tctatctatt aattatctat tggactatta aaattttaca gactaattac
     8521 atatagtcaa ggtaatcttt taattttcgt gctctggtag gatggcgcaa cctccttaaa
     8581 gcctttgctt ctatctggcg tatacgttca cgggttactt taaacacata accaacctct
     8641 tccagcgtat gcgagtagcc atcatccagc ccaaaacgca tacggataac cttttgttca
     8701 cgtgctggta atgtgcttaa aacagcattt atctgttctg acaataagcg atatgctgct
     8761 gtattaacag gtgaatccac ttctttatct tctataaaat cacccagtag cgagtcttct
     8821 tcttcgccaa tgggggtttc aagcgatatt ggctcacggg caacattttt aacacctttt
     8881 accttgctca catgccagcc taatctatcg gctaattcat ccggacttgg ttcacggcca
     8941 aactcctgtt gaaacaaccg tgtttcacgc ataaccttat taatttgctc tatcatatgt
     9001 accggaatac ggatggtacg cgcctgatca gatatagctc gtgttattgc ctgacgtatc
     9061 caccatgttg cataggtgga aaatttatat ccttttcgat attcaaattt atcaacagcc
     9121 tttatcagtc caatattacc ttcctggata aggtcaaaaa agtgcatgcc acgatttgca
     9181 tattttttgg ctattgatac aacaagtctc agattagcat tgataagttc cttctttgcc
     9241 agtgatattt tccgatgccc tatttcaatc tgtgttcccc attccataat ttcgtcacag
     9301 gttgaaccag cttcctgctc aatcctgcgt atcttccgct cattgtttct gatatcctta
     9361 actatctcta aaagctcatc tttattttta atcttcagct cttttaatat cttttttaca
     9421 tcttcattct tttctaattt acgcccaaac tgcttcagct ctttgacagt tttgttaaaa
     9481 ctgttttcca gattattaaa aaaatgacgg gtttctttta tacgggcaac cattgataat
     9541 attcgctgtg cagttttatt tatctcattt tcatgcacct gtaagtttat caatgcctcc
     9601 ctgatactac tgcgggatgc catcatcttt tctttcaact ctaatgcttt ttttgaatct
     9661 tcagataact ttttaagttt ggcttgactt tgcattatct ttttatcttc agcaatgata
     9721 atgctcatat tattttcata tcgtttttca agatcgtgca tatcaaacga agaaaaatag
     9781 tacagcttgt taatacgcaa aatatcagtt aacttaatct taccgctttt tacttttgag
     9841 tgatttttaa caagttcatt gagcagcacc gttgagtcca gaacagcatt ttcaattaat
     9901 atttcacctt cttctattcg ttttgctaat ttaacctcct ggtcacccga taaaagcgac
     9961 accttcccta tttctttcag gtataaccgt attggatcat ctgaaacgcc tgtttcagat
    10021 gatgaagaag atgatttttt ggaatgcgtt ttttcttttt ttaatggtgc agcatcattt
    10081 cgtcgagttg gttcttcaac aacatcaatg cccaattggt tcaatagaat aaaaatatca
    10141 tcaatcttat cagaatttaa tatcttgtcg ggtaaaatat cattgagctc atcataggta
    10201 atttcaccat ttgatatacc caattccaca agttttttaa cctccggaat gctctctaaa
    10261 agtacatcgc cttttgccat aattaattct accctttaca ttaaaattat tattacttac
    10321 aaaatcattg ataacaatca caagaattca tacaatccct gtattgatgt tataaacaac
    10381 tactatttca ttaatgtttg atacttcctt tattgtagat gtacgaagtt aacttttctt
    10441 tttcacgtcg caatacttca atctcggtta tataatattg tatattctca cgattgtcct
    10501 tttcaatttt ttctgcaaat tcattaattt ttttgtcgat tgtgaaaagc ttcatattta
    10561 gtataatttc cgaatatgca acatgaggat cttcaaatga aaattgtttt gaaaagccct
    10621 gtgtcagcaa ttttttttct tcaccttcag gataaaaatc aaacatcttt tccagtgaaa
    10681 aagattcatt gtcttcatac aaagcaatca atgtctgata gatattcttt gctacagtat
    10741 cagtaaaatc ctgtaatggg caatcaacaa tggcattttc aataagcagg ggataattgc
    10801 ataaaagtaa taccaattcc cgctggcttt ttgcataaaa atcaacagta ttattatcgg
    10861 taactatcac cccattagtt gttttacctt ttgtaaaacg ttcataatca gctcgtattg
    10921 ccttttcatc aacctgaaac actgaactta gctttttaaa atagtgctgc cgttcaacct
    10981 caaatgggat agtacgtaaa atgccaaaaa tctgcttcag tacattaacc tgtgattttc
    11041 ctttattttc tgcaataatc cgctcaatgc gaaaatcaac cggcttgaga gcactatcaa
    11101 caacagccat aaattcccgt gcaccacgtt tgttgacaaa atcaaatggg tccatctcgg
    11161 gcaactgcgc tatttttata gttatgttaa ggtttgctgc aacgtcaagc gaacgcaacg
    11221 atgcctttac ccccgctgaa tcggcatcaa aaacaataat tacctcattg ctcaggcgcg
    11281 acagcagttg tagctgctcc tgcgtaaggg cggtgccaag cggtgcaaca acattctgaa
    11341 tcccagcctg atatactcca ataacatcaa gatagccttc aacaacaata gccctgccca
    11401 attcagcaat gtgctgccgt gctttgttga atccatacag aatattccgc ttctgaaata
    11461 ccagtgattc gggagaattc atatattttg gctcgccaga ttcaataatt ctaccaccaa
    11521 aaccaattat ttcatttttt tggtcaaaga ttggaaatat taccctgttc cggaacctgt
    11581 cataataatg ggttatagta ttttttaccg atttattgat attaccggtt ttaacggcta
    11641 tatcaagcgg aacattcttc tgtcgcaaaa tatcggttgc atactgccat tcatcaggtg
    11701 catatcccaa ttggaattcg tcaataccct gttgggtaac cccacgatgc aagagatagt
    11761 ccagagcacg ctttcctgcc gctgacatca acgcctgacg gtaacgctct gctatcattg
    11821 catttattgc cagtaatgtt gtatattcat cgttttgctt ttcctcatta cgaacaggaa
    11881 tgccgacaat ctcgccgaca atctttacac tttgaacaaa atctacatgc tctacctttg
    11941 ctatgaaggt gaatacattg cctccctcat ggcagccaaa gcaataaaac atctgcttat
    12001 caggcgagac agtaaatgaa gggcttgttt ccttatgaaa agggcacaga ccaacatagt
    12061 tcttaccctt ttttgtaaga gtgggaacat aacgccgtat gatatcttct atggcagcct
    12121 tctgccgtat atgttcaatc gtatcgcggg gtatagccat ttgttataaa tatatggtgc
    12181 aatctacaat ttttcaagtt tttgagcaac tatttgtttt acaatctgcc cgtctgcttt
    12241 ccctttcagg tctttcatca cctgccccat gaccttaccg gtatccttat tggtgatagt
    12301 tcccattgaa gcaatcactg catcaacatg agaggctatc tcatcagcac tcatctgctg
    12361 aggtagatac tgcatgatta tctcaagttc ctgcttttct ttacgagcaa ggtcatctct
    12421 gccggctttt tcatattcag ctattgcttc ttttcttctt ttggcgctgc gtgttaacac
    12481 ctcaatgatt ttttcatcgg taagctcctg cgtactctta gtcttttcat acagtatgtc
    12541 cgatttcatc atacgcaagg tactgcttaa cagttcattt ttgcttttca ttgcgtcttt
    12601 aaggttggat tcaattgtgg taaggatgct catagaaata cctcactgca atagtatttg
    12661 atgaagtgac gtatacagag gagagaattg tgacatataa tcacagtata tttgtatgtc
    12721 aacaaaagtt tcaaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt
    12841 attgcaatgt tttcatacag cagtttttgc cggttaaaat ttgctgtatg cagggttgta
    12901 gtaaagggtg cacaccgttt catggaaagc agcaaagctc atatactaca tgcagataat
    12961 cttgagtgta cacagattac cggaactatc gccaaccaat agtgctaaaa catggtgatg
    13021 aagccatcca gccgtgttat gtggctacaa attgcatgta acacttcttc gtgggcaaac
    13081 agcgcagtaa tggtatatga aataaatttc ccttgcttgc tttcacgttc agtaatgtgt
    13141 gcatcaatag tattctcggc acaaatattg atgagcatct ctttaagaaa tagagtgttg
    13201 tgaaaaatca ccttaaacgt tatctctcgg ggaaattcat cattgctatg gttttcattt
    13261 gccatcatag cacgccacac tgttcatgaa gcatgcaagg taagcagttc atatcctgtt
    13321 atattcagct cgtcacgcat ggctacaagc tctttcatta cttcgttatt gaccggcaca
    13381 cccttatctt tgcgctccag gtaagcgagg tattcctttt cgcctgccgt gtagattttc
    13441 ttttgacctg gcattctggc tgaattgcgc aactgacgca ggatatcacc ggtaattgat
    13501 ttgaacgatg caatgtcggt aaacgcctca atagcaatag caataaagaa atgtcctaaa
    13561 tggtatggca ctttattacc ctgcatgtca aagccgctga gcatttttaa atagctcccc
    13621 gcctgcagtg ccgccgacag tatctcaaca acagtacaat agccataccc tttataccct
    13681 gcgccatcct cgccaatacc gcccaatggg gtcagagcca tgctcccttt aataagccca
    13741 tcaagtacct gatgagggtt agtacaggaa ttgccatggt tatcgataac ccatcctggg
    13801 ggaagaggtt tgtcaaggcg ggcatacacc tcgattttgc cgcgttgact tacactggtg
    13861 gcgcaatcaa gcaaaaacgg aaaattctca tcagcaggga tgccaaaggt aagagggttg
    13921 gtacccagca tgttttcaac gccaaatgtt ggcgccaccg aaggccttgc attggtaccg
    13981 gttatgccta tcatagcatt gtcagcagcc attttggcat aatatccggc aatgccatag
    14041 tgagtggaat tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nggcaaaaag
    14161 gtcaagctct atttttatag tatattttat atcaatcttc ttttctttgt gtataggtcg
    14221 gtaagatttt tttcagaaat tctgaaagcc gttccatgat tcctgacacc accataatct
    14281 tttaaatgca ttcgcaaatc aatcatcaat tcatttctgt taaatccttc caaaaatttt
    14341 tcaggtgttg gattttctaa aatataagct tcattataat gaaaaaattc tttctcatca
    14401 accattttag aatctgctaa tatcaatagc aatctatcta gtttagtcat aaatttacct
    14461 gcaataacat aggtactcca ttctgcaatg gtttcatctt cctttgcatt tttgagcaaa
    14521 acaagatgct tgtcattatc tatttctaaa aagaatcctt gaacattatt aacttttgca
    14581 ctaacagtag catataatga ctgtctcccc tgtgcatctc tatagccata tttatttata
    14641 aggtcttttt gcttcattct ccatacacct ctattgaatg taaagagagt aattaaagag
    14701 ttggtgtttt ttcttgttgc tttaatttca atcctacctc ctatatctgg aattgttata
    14761 ttggtttcac taagccctag ttctttttca agtaaataac caacagcggt tgggcctttc
    14821 ctaattgtct caataaatcc tttactcttt atatcttcta aatttttcac aagttttttt
    14881 agttgcatac tttatatctt tttacatata acgatgtatt catttttcat tgtttgccct
    14941 gtttgtccag gaatattagt tgggctattc atcagaggca tccttttatt aggaatattt
    15001 ctaacaatgg tttccatatg tacaaaatta ttctgctcaa acaattgtgc agttatttta
    15061 ttggttggaa tattaatatt ttttactgtc ctattgccaa caacatagca aacaactcca
    15121 cctttcttta tcgtagcaga tacattaaca atagatgatt cataatcttt aaaaaacgaa
    15181 acaacctcca tagctcgttt gttatccttg tttgatattg cagtaataac atcattcaac
    15241 aaatcgttat taaatttaaa attatttaca ttatttttcc ttccacccat gagcaaatta
    15301 tctattttat ttgcattgtg gaatcccaac cattcatttg caaatcttga aaactgccca
    15361 taagctacag tagttcttga atctccatag ggtggtgatg ttacaactat atcaacagat
    15421 tcttgtggca ctaaatcata tggaatagac agcacagtat taaatgaata aatgtgtgaa
    15481 actacattct gagggcaaac cttaataaag ttaataagtc cttccttgtt tcttgacaat
    15541 tttcctaata taatcccaaa aacatctaaa tcttcatttt taattaattt tttatctcgt
    15601 actaatttaa attcaccatt cttggtattt gaagtttctc taatagtttc actaaaagct
    15661 actttaaaaa aattttggat aaatacattg tctatttttt gaataatata attgaatatt
    15721 attgccagct tatattgcac tgactttgaa aaccatatat caatatttgt tatgcatggg
    15781 agtgttatag tactatagtc ttcctctttg aagcttttgg aaaacaaata atttgtaaaa
    15841 tcatacaaat atttatccaa tactttacta tctattttgg tagtttttgc ttgagcaatt
    15901 aatcgagcta atggattgag gtcagtccct atagcattta tcccttttaa attggcttca
    15961 actaaagaag tacctgtacc acagtatgga tcaaaaagaa gcgttgcgtt ttccccataa
    16021 atttcaatta ttcgccgcgc tacttgaggt atcatcatag ctggataaga atgaaaacaa
    16081 tgtgtatatt cctttgtatt cgcattttta aaatcccaac tatcctcata caatgtatta
    16141 tttttttgta tgcaattgtt acaagataag ttgatagatt tatccaataa tttatcttta
    16201 ttcataactt gaaatttgat tttatagtat aaataacatc tccaatatga aaatagtttt
    16261 ttatatttat tctgtcaata tttttaattt ttatggaatg gatttatatg caacgttgct
    16321 acgactttcc ttccaataat aattgcatac tgcaaagaca attgatcagc aaaacaaatt
    16381 catatatgaa ctatcgcaca taacgaacga tgtgtttttt taatttgtgc aagtttttat
    16441 aaaaccacaa tgctacaata gcacatcccg tcccgcacaa caacaatgtt tgggcaacgc
    16501 ctattttctc ggcgatagct cctgcaatga ggttaccgat gggagccatt ccaatatgtg
    16561 acatgccata caggctcatg accctccccc tcatggtgtc atcaaccatg gattgaataa
    16621 atgtattgca ggatgccatc tgtataatta aacttatgcc aatgataaaa attaaaatca
    16681 atgaacaata caggttccgt gaaaaggaaa agcaaaccag tgtgctcccc agcaatagtg
    16741 atcctgccgc tatcttgttt tcaaaccgcg tgatactgct gcgtgaagca ataaacagag
    16801 caccaacaag cgccccaaat ccagcagtgc ccagcaaaaa tcccaatgta tgcgaatcac
    16861 catgtaatat cttcactgct acaaccggca tcagcaccat aaacggcatt ccaattaagc
    16921 tcatgagccc taaaagaata agcacgcttt tgataggata atgatttttt acgtatacaa
    16981 aaccttcttt tatattttcc cagaaattgc tggtatgcaa aatcttagaa taatgtatat
    17041 ccttcatgag caataatgcc agtataatcc ctgcaaatgt tgttgtgttg ataaggaagc
    17101 acaaaccttc cccaaactgt gcaaccagaa aacctgccag aggcggtcct ataaggcgcg
    17161 tgctgttaaa tactaccgaa ttgagcgcaa tggcattgcc taaaagctgt ttatcagcaa
    17221 ccaggtcaaa taaaaaggac tgtcgcacag gtatatcaaa ggcattaata caccccattg
    17281 ccaggttgag taacacaatg ctggctggag taatataatt ggatataaca agtaatccaa
    17341 ggataagcga ttgtatgcat agcatcgttt gggtaatcat aaagatagca tgcctgttga
    17401 aacggtcggc aacaactcc
//
LOCUS       DAOOJH010000047        17115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3245_length_17115_cov_9.737339, whole genome shotgun sequence.
ACCESSION   DAOOJH010000047 DAOOJH010000000
VERSION     DAOOJH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17115
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3245_length_17115_cov_9.737339"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="obgE"
                     /locus_tag="PLJ75_07505"
     CDS             complement(<1..656)
                     /gene="obgE"
                     /locus_tag="PLJ75_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPD05098.1"
                     /translation="MAKFTDTITIKIKAGNGGAGAVSFRREKYIPKGGPDGGDGGKGG
                     DVIIQAQQGYYNLGHLFKDRIYKAKNGGYGMGQNRHGKDGQDLIIKVPAGTEVFDTQT
                     GEPLADLVNEGDSVCVAKGGIGGKGNAFFKTSTHQTPRFAQPGMQGDERTVTLNLKLI
                     ADVGLVGLPNVGKSTLLAKITNAQPKIANYPFTTLIPNLGVIEQGEKVITIADIPGII
                     "
     gene            complement(1057..1317)
                     /gene="rpmA"
                     /locus_tag="PLJ75_07510"
     CDS             complement(1057..1317)
                     /gene="rpmA"
                     /locus_tag="PLJ75_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPD05099.1"
                     /translation="MAHKKGGGSSRNGRDSHAKRLGIKVSGGQFVKSGSIIIRQRGTK
                     LHPGLNVKRGSDDTLFAVADGVIKFERLGKERKKVSVYPVQS"
     gene            complement(1363..1716)
                     /locus_tag="PLJ75_07515"
     CDS             complement(1363..1716)
                     /locus_tag="PLJ75_07515"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HPD05100.1"
                     /translation="MINVTLQGIILRDGNIALSGSEIVITITGHSTKAAKGNDIVCAG
                     VSALAQSVVVALEYWAIVHSIVQDDGLLRFSIHVPLLNDREITRCESIVSVLVIGLNE
                     IRKQYSQFLTINVNE"
     gene            complement(1716..2030)
                     /gene="rplU"
                     /locus_tag="PLJ75_07520"
     CDS             complement(1716..2030)
                     /gene="rplU"
                     /locus_tag="PLJ75_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016525666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPD05101.1"
                     /translation="MYAIVEIAGKQYKVEKDKTVAVDKLDAKDNNEIAIDKVIMFVDG
                     EKVLIGQPYLSNVVVKAKVDGMVRGKKVRGIKFKRRKNYTRTFGHKQHYTSITIVDMS
                     VQ"
     gene            complement(2124..2197)
                     /locus_tag="PLJ75_07525"
     tRNA            complement(2124..2197)
                     /locus_tag="PLJ75_07525"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Ile,seq:cat)
     assembly_gap    2846..2945
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3357..4430)
                     /gene="dgcA"
                     /locus_tag="PLJ75_07530"
     CDS             complement(3357..4430)
                     /gene="dgcA"
                     /locus_tag="PLJ75_07530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HPD05102.1"
                     /translation="MNDDVSLYGEEIKFPAEIEQYERKIYDLKQLIEISKGLNSTLDY
                     NILIDSILLTCMGQMQLLKAGIFTHRSIDRQEFVLHRNYKGFEIDHKIEYLIPANSKL
                     IQFLDMNFKCYTLDELKDILRDEPSLESLICLDPSLIVPLKGKGTLNGIIILADRINQ
                     KDFSESDKEYMLNIASLAGIAIQNAYLYELATTDMMTRLKIHHYFQTALMEEREKSIK
                     SSLPLSLIMIDIDNFKDFNDTYGHTCGDLVLKNVAMVISGSVRQSDIASRYGGEEFAV
                     ILPHTEINEAFKVAERVRTSVQKSYILYKRKKLGVTVSVGVTQFNPSLDSTSKNIIER
                     ADKALYMSKQQGRNKTTVLLTDK"
     gene            complement(4442..5161)
                     /gene="hisA"
                     /locus_tag="PLJ75_07535"
     CDS             complement(4442..5161)
                     /gene="hisA"
                     /locus_tag="PLJ75_07535"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPD05103.1"
                     /translation="MLVIPAIDLKEGRCVRLVQGDPLQEKVYSYDPVTIARQFEDAGA
                     QLIHVVDLDGAFEGQPVNMHIVKEIAKSVSIPIEIGGGIRTEAAIKMYLDEGIERIIM
                     GTILIEESFREMIQRFKEYIIAGVDARDLHVATHGWKKIIDINAIEFIKDIQNIGIAE
                     IIYTDIATDGMLQGPNFRSIERILADVPGVSLIASGGISTMQDIQKLLHYSGSGLKGC
                     IVGKAIYDGMVDLRSAIKLCS"
     gene            complement(5152..5760)
                     /gene="hisH"
                     /locus_tag="PLJ75_07540"
     CDS             complement(5152..5760)
                     /gene="hisH"
                     /locus_tag="PLJ75_07540"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPD05104.1"
                     /translation="MITVIDYGMGNLRSVVKAVEKYTSDVQVSSDPRSIASSKALIMP
                     GDGAFGQAMENLTQSGWIEPLVRFIGNGGYFFGICLGYQLLFTSSEEFGNHKGLDIIP
                     GTVIRFPDDLKVPHMGWNQVELIKEHPVVNGIPQNSYFYFIHTYYPVIGDSSWIMGQT
                     GYGVTFPSIVGRGNCIATQFHPEKSHRYGLTMIENFVRMVCS"
     gene            complement(5757..6341)
                     /gene="hisB"
                     /locus_tag="PLJ75_07545"
     CDS             complement(5757..6341)
                     /gene="hisB"
                     /locus_tag="PLJ75_07545"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011064953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPD05105.1"
                     /translation="MERSAHIERKTNETDITLKCILDSGDVSTITTGIGFFNHMLTLF
                     VTHGRMNIQLNARGDLEVDDHHTVEDIGICMGKAFNKALGDKSGISRFGQAVIPMDEA
                     CTAAVVDISGRGCFVYKGDKLTGAIGQYQEELTLEFFQSFAHNALITLHIQQMYGDNR
                     HHIHESIFKSAGLALYRAYSLSGRTGIPSTKGVL"
     gene            complement(6378..9251)
                     /locus_tag="PLJ75_07550"
     CDS             complement(6378..9251)
                     /locus_tag="PLJ75_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05106.1"
                     /translation="MKKFFKHKICYSAILCVIITVPLFVYPFGKNKVTVEVFTWHILR
                     TIHFNVYYPYGIERLAATTATIAERAYGYLADTLQHQLNEPVPIIVFPSHIDFQENNI
                     LMQIIGEGTGGFTEAFKNRVVIPFSGSYDEYQHVLVHELVHAFQFNILMHDSSGKLQS
                     MMSMGAVPLWFTEGMSEYLSIGFDETADMTMRDFFFNDQYVSLMDFTTYRTGNLYYYY
                     KVGQAFFYFFETVYGKGKIGEFLRDIRDMGNFEKALKVHTKKDLEAIDKEFRHFYKKR
                     YYPLMVGRNFDEEEGERVTDHKKTRSVFNTCPAISPDGKKIAYIDNRDIYASISVITI
                     GEKEAEAKARVLLKGNETSQFEGMHLLDNYLSWTPDGKGIVFVSQSNNRDVIFILDSR
                     NGTIIKKIVLPFRSIKDPALSPDGNYISFIGQTNTQSDVYIYRLNDTMLQQITNDVYT
                     ERYPRLDKKNAFIYYSSNYPTNDYTGTDYAIIRHNVLTGERQVIVKAQSKNLQVDVAG
                     DGNTIVYISNRDGIYNVYRYTIATGEEIKITTGTTGIFYPRLFPEGNAFAFVSYQNGG
                     YDIFIKNEIKPVTETPLFNSEHIPVMLPPNYFPLSKAVLDNYETRISSDWVFVGLGGT
                     VGYGFTGFAQMSFSDYLGDNRIIVTTDFLRDDESNNFMNYDVQYLYLRYRWDWTFGCF
                     RQKSPYGIFTLANLNDIIHNAYWSTLSMDHYGAYVIASYPFTKFSRFSTRVSSSRYEQ
                     DYGYSDERPDVYGNLNSISVSYNYDNVLWGFLAPLRGTRGQIMLTQVFNLTGQDYSFT
                     SIDIDLRHYFFIDKQYVIALRGVGGKIMGSEKEYFKYYIGGFNTLRGHPFVEYGGSNV
                     FLFNAEFRFTFIESINMGWPLFLKIGNIGGVLFVDAGSAWDNSYTFFNNETDQFEDFK
                     MDMGFGFRLTVYPIVILKLDYAWPYYYGEFGEKEILFSLGYEF"
     gene            9408..9591
                     /gene="ssrS"
                     /locus_tag="PLJ75_07555"
     ncRNA           9408..9591
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLJ75_07555"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            9610..10656
                     /locus_tag="PLJ75_07560"
     CDS             9610..10656
                     /locus_tag="PLJ75_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05107.1"
                     /translation="MEIITVFGVRWRVAPLWEVLVTLFVILVIIGFFALLRYNQYLKL
                     KKSSIEQLFIYKARQRGLTGYQIRILQHIISLSKLSTPEIIFNNVTIFETGLVKFLQY
                     IKKSRDTFNEDLKDIFKDIVITYERIYNPVEYQEQLNSPDELTTNTMVCGYSATGRAF
                     IAKITQITGDAIHCKYFRKPEDIPADIIAEQVTIFFFRNGDAEYSFDSKIIAIQENTI
                     VLQKPQAFKRGKEVHHPYIQTLIPCTVQLKNDESKNSEEVIIQHDETVSINGTIERLN
                     EYEAVIRVEKRLEYQKEYILFFTLENFKIEVLSTIIASRTITREKIIYYTFKFVQMSE
                     AAKNVISKYVKERL"
     gene            complement(10668..11786)
                     /gene="dnaJ"
                     /locus_tag="PLJ75_07565"
     CDS             complement(10668..11786)
                     /gene="dnaJ"
                     /locus_tag="PLJ75_07565"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPD05108.1"
                     /translation="MATTKRDYYEVLGVPKNATEDEIKQAYRKLALKFHPDRNKGSKE
                     AEEKFKEATEAYEVLRDPKRRAQYDKFGHEGVAGFEGFGRGAYTDFSDIFGDFDIGDI
                     FESFFGAGSGSGTRTRSSRTRRGRDIQYDLSITLDDAANGKEVQIEIPRHEVCSSCGG
                     TGSASGSKPTVCPVCNGTGQIRQTQGFFSVTQTCYKCRGEGKIITSPCRACRGTGLEE
                     KHRKITVKIPAGVESGSRLKISGEGEQGPGGGSAGDLYVVVHIQKHPLFERHGNDLIC
                     VVDIPFTIACLGGEIEVPTIYNKKAKMKIPAGTENGQIFRLKGNGMPYLGSYGKGDQL
                     VKINIKVPKKLTPRQKELLKEFARIEGETVDTDREFYS"
     gene            complement(11868..13805)
                     /gene="dnaK"
                     /locus_tag="PLJ75_07570"
     CDS             complement(11868..13805)
                     /gene="dnaK"
                     /locus_tag="PLJ75_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPD05109.1"
                     /translation="MTMSKIIGIDLGTTNSCVAVMVGGEPVVIPNSEGQRTTPSVVAF
                     TDKGERLVGQVAKNQMITNPENTIRSIKRFMGRSYGEVQNEIKMVPFTVADDGKGGVK
                     IKTIAGEFTPQEISARILQKMKQSAEDYLGEKVTRAVITVPAYFNDSQRQATKDAGRI
                     AGLTVERIINEPTAAALAYGLDKKERNHKIAVYDLGGGTFDISILELGEGVFEVKSTN
                     GNTHLGGDDFDQRIMEWLISEFKKQTGIDLHTDKMALQRLKESAEKAKIELSNKLQTE
                     INIPFITADQNGPKHLIMTLTRSKFEGLVEDLIESTKEPCIKALEDAGLTPGDIDEVI
                     LVGGSIRIPAVQELVRRVFGKEPHKGVNPDEVVAVGAAIQGGVLAGEVHDVLLLDVTP
                     LSLGIETLGGVMTKLIERNTTIPTRKSQIFSTAADNQTAVSIHVLQGERELASQNRTI
                     GRFDLIGIPPAPRGVPQIEVTFDIDANGILHVSAKDLGTGKEQKIRIEASSGLNEEEI
                     KKMVKDAEQHAEEDRKARQLVEARNEADALIYSLEKLIKENEGKIDAAEKSSIESEIE
                     NLRKAMESSDINVIRDAKAKLERASHSFAEKLYKQGAEQYAQSQSQQQSDTSGTTEES
                     TQGGEKVYDADYEVVDEDKKK"
     gene            complement(13875..>14123)
                     /gene="grpE"
                     /locus_tag="PLJ75_07575"
     CDS             complement(13875..>14123)
                     /gene="grpE"
                     /locus_tag="PLJ75_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPD05110.1"
                     /translation="EEINSLGQAFDPEYNEAVEIDESPDVVKDTITKVYQKGFKLDKY
                     VVRAARVKVTRPAKQKVEDDNANNANNATEENTAASQN"
     assembly_gap    14124..14223
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14224..14507)
                     /locus_tag="PLJ75_07580"
     CDS             complement(<14224..14507)
                     /locus_tag="PLJ75_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05111.1"
                     /translation="MSNKHDKRHKREDDNIHDAINQEKIQTMNEEKIKVQENGDSSLN
                     QSAGNDQVESSSELIQQKEDQLRKLQEELNKKNEELDALKDLLVRRQADF"
     gene            complement(14553..15596)
                     /gene="hrcA"
                     /locus_tag="PLJ75_07585"
     CDS             complement(14553..15596)
                     /gene="hrcA"
                     /locus_tag="PLJ75_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HPD05112.1"
                     /translation="MHTKQLNDREAQVLKAIVYEYIATGKPVGSRSFVQKYSFSLSPA
                     TMRNIMFDLEQMGYLSHPHTSAGRIPTDKGYRFYVDSLLDTYETVAKTDIEVREEMLA
                     REIELDKIFMSIVKMLSLISKYTGIALMPKPDFTVVKHIELISLTSNDVLFIIVTRTG
                     IVIHKKVKLSSSINQDDLYKYSRFLTAELSGYSLVEIRQSLINQLRTVTDVETSVRDV
                     ALDITELALKDEQEPDIYIDGIENLLHIPDIVEAEKLHEIVRFIEQKDTLRKIMEDLR
                     QKEGVFTLIGDEIKDIAIPCCSIIASSYKIGNSPVGVVGVIGPTRMDYEKVVPLVDYT
                     GKVVSNFLTQMTK"
     gene            complement(15847..16656)
                     /locus_tag="PLJ75_07590"
     CDS             complement(15847..16656)
                     /locus_tag="PLJ75_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05113.1"
                     /translation="MPVTKAEKVAYNDFVKGYRAKLEELTKQIKEAEQKKKKMPGIKG
                     YINLELILLYIKYIYTLLNMNTVSVNMLKVKNERFLNDARKEFYRVLQLFEEIVGKDI
                     DRMLKENEEYLAAINKLNPQQTLALVKEIHVLLQKIMDALGESSKWKWSFVELQGRVA
                     VITKNMINFSELQKYRDPRKAFYYERQDMLKLCKQSLSEAARQYRNKYELSTKSSEDI
                     LKSIELLTALRRIHILFGESEEAEKLKNTIEALRLRLEADEKEEEEKKKKK"
     gene            complement(16668..16814)
                     /locus_tag="PLJ75_07595"
     CDS             complement(16668..16814)
                     /locus_tag="PLJ75_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05114.1"
                     /translation="MEIMRISGADVVPAGPAVNNQGQQENTVEKEKPEQPEKVNKSTI
                     DTYA"
BASE COUNT         5046 a         3695 c         2627 g         5547 t
ORIGIN      
        1 tcaataatcc cgggaatatc agctatcgta ataaccttct ctccctgctc aatgacccct
       61 aaattgggta taagcgtggt aaaaggataa ttggcaattt ttggctgagc attagttatt
      121 tttgcaagca acgtagattt cccaacattg ggaagcccaa ccagcccaac atcggctatt
      181 aatttaaggt ttaacgttac cgtacgctca tcaccctgca taccaggttg agcaaatcgc
      241 ggtgtttgat gggtagatgt tttaaaaaat gcatttcctt tcccaccaat gccacccttt
      301 gcaacgcata cactatcgcc ttcattgaca aggtcagcta agggctcacc tgtttgtgta
      361 tcaaaaactt cagtacctgc aggcacttta ataattaaat cctgcccatc tttgccatgt
      421 cggttttgtc ccatgccata tccgccattt tttgctttat atattctatc cttaaacagg
      481 tgccctaaat tatagtatcc ctgctgtgcc tgtataatga catcaccgcc ttttccgcca
      541 tcgcctccat caggaccacc ttttggaata tatttctcac gcctgaacga aacagcacct
      601 gcaccgccat tgccagcctt aatttttatc gttatagtat cggtaaattt agccacaaat
      661 cattccttac gtttttgata gataatagca taaaaggaca gcacttaata ctgcacatgt
      721 atttattaat cactatgtta ctatagtatt atttgttata ttactgatag tatcgcaatt
      781 gatgtatttt tctaaaattg agtttatagg ctaccccatt ttcacttttt cttcgtcaca
      841 acatgataat gtttaccctt gagggagcag tttcaacgat agagtaagga gatttatgca
      901 atgcaaagga gtttactgca aacactattc cattgagcag tatgtacaaa aaagaaaaag
      961 gggccacagc ttaaaaactt acccctttta ttattcattc aataacttta ttcaatagct
     1021 ttttaatata caattacaat tacactggta acgtaattat gattgtacag gatataccga
     1081 aaccttcttc cgttctttac caagccgttc aaattttatt acaccatcag ctaccgcaaa
     1141 gagtgtatca tcactaccgc gctttacatt taaaccggga tgaagttttg ttcctctttg
     1201 tcgtataatt attgagccac tctttacaaa ctggccacca gatactttta ttcccaaccg
     1261 tttagcatga gaatcacgac catttcgtga tgaaccacca ccttttttat gtgccatgta
     1321 ttcacctctt atttacaata attatcaaaa tagtatcaaa atttactcgt taacattaat
     1381 agttaaaaat tgtgaatatt gttttcgtat ttcatttaaa ccaataacta aaacgcttac
     1441 tatactttcg cagcgggtta tctccctgtc atttaataat ggaacatgta tagaaaaccg
     1501 taacaatcca tcatcctgaa caatactatg aactatcgcc caatactcca gagcaactac
     1561 tacggattgc gctaatgctg aaacaccagc acaaacaata tcattgccct ttgcagcttt
     1621 tgtagaatgt cccgtaatcg ttataacaat ttcgctgccg gataatgcaa tgtttccgtc
     1681 acgtagaata atcccctgta aagtgacatt aatcactact gcacagacat atcaacaata
     1741 gttatacttg tatagtgctg cttatgccca aatgttctag tataattttt gcgcctcttg
     1801 aattttattc cgcgcacctt tttaccccga accattccat caacttttgc ctttaccaca
     1861 acgttggata aatacggttg cccaataagt actttctcac catcaacaaa cattataact
     1921 ttgtcgatag cgatttcatt attatccttt gcatccaatt tgtcaacagc tacagtttta
     1981 tccttttcaa ccttatattg tttcccggct atttcaacta ttgcgtacat acacctctcc
     2041 acgatttttc aatttaccac tttattgcaa tcttatatta ttaaatacag aaaaaacaat
     2101 aatagaggat ttagctctac tcttgggccc acagggactc gaaccccgga cctactgatt
     2161 atgagtcagc cgctctaacc tgctgagcta tgggcccctt gtgaaatgac aaaataataa
     2221 tataccatac tacatgtcaa gaaaataaat ttcaattatt taaaaagtgt ttaataggta
     2281 tatcattgta ctatactata gcttttttat agtacatttt caatgacaaa ttgtttataa
     2341 aagtgttatt ttttaaaatt tattttaaaa tcattatcca tttactatat tttaccatac
     2401 tatgaaggga aatcagtatt gacactcatt atagctgtta attatttaaa tatatagata
     2461 aagaacaaca aacatcattc atggtaaata attgactata tcttagttaa gtaggcaaat
     2521 cattatttgg ggaactaatc acaagcaaat tcatatcaca aaataataaa cccatcagat
     2581 aacgagagtc gagataacta tacatacaaa aaattcttag aagccttata gattgatggt
     2641 ctgcatgctg atgcaaaagt attactttaa ggtattaata aagattttga ttcccataaa
     2701 gtagctactt cttagcaata taaggacatg gagatctttc agccccaaaa tagtattgct
     2761 gttcatttat ttcaatagca tcattatatg cctcaactat gcccaccaca tgaataatag
     2821 ctcccactac aaataaaagt cctgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnactgg ataaataata aaattaatag atagcgttag tattaacata cccgatatga
     3001 acttaatata atagctgata gttttgtttc ctttattttt gtttaccatg ctaaaatttc
     3061 tccaaaaata aaattatcac cacattgtca ttctgaggaa cgcggtccct tttatcattc
     3121 ttaagggagt aacacagctg aagaacctca tcttccaaaa aaaattattc gccattgctc
     3181 ataatgacat tgaacattat tgttgttcta agcttgtctc agaatttaat tccttaacct
     3241 tcaatgcatg aaactactca tcataccatt gcactatttc acttattact attttcgtat
     3301 tatggcagga aattatatag tgatacaaga caaaatcaag cattttattt tattttttat
     3361 ttatccgtaa ggaggacagt tgttttgttt ctaccctgtt gttttgacat atagagtgct
     3421 ttatcagcgc gttcaataat attttttgag gttgaatcca gagacggatt aaactgggta
     3481 acgcctactg atacagtaac acctaatttt tttcttttat agagaatata tgacttctgc
     3541 actgaggttc gcacacgctc tgcaacctta aaggcttcat tgatttcagt atgcggcaat
     3601 attacagcaa attcttcgcc gccataccgt gatgcaatat cactctgtcg taccgaacca
     3661 gatatcacca ttgctacatt ctttagaact aaatcaccac acgtatgccc gtaggtatcg
     3721 ttaaaatctt taaaattatc aatatcaatc attatcagtg aaagaggaag gctgcttttt
     3781 attgattttt cgcgttcctc catcaatgcg gtctggaaat aatgatgaat tttaagcctt
     3841 gtcatcatat cagttgtggc taattcatat aaataagcgt tctgaatagc tatccccgcc
     3901 agcgaagcaa tgttgagcat atattctttg tctgattcgc taaaatcttt ttgatttatt
     3961 ctgtcagcta atatgatgat gccattaagg gtaccttttc cttttaacgg aactatcagt
     4021 gaaggatcaa ggcaaataag gctttccagc gaaggttcat cccgtaatat atctttcagt
     4081 tcatccagtg tgtaacactt gaaattcata tcaaggaact gaattaattt tgaattggca
     4141 ggtattaaat attcaatttt atgatcaatt tcaaaacctt tataatttct atgcaataca
     4201 aattcctgac ggtcaatact gcgatgagta aaaattccag cttttaaaag ctgcatctgt
     4261 cccatgcatg ttaaaagtat ggaatcaata agtatattat agtccagagt ggaatttaat
     4321 cccttgctta tttcaataag ctgctttaaa tcataaattt tgcgctcata ttgctcaatc
     4381 tcagcaggga attttatttc ctcaccatac aatgatacat catcattcat attgccacca
     4441 tctaactaca cagctttatt gcactgcgta aatctaccat cccatcatat atagctttac
     4501 caactatgca accctttaat ccgctacccg aataatgcaa caatttttgt atgtcctgca
     4561 ttgtagaaat accaccagag gcaattaacg aaactcccgg gacatcagct aatattcttt
     4621 ctatcgatcg aaaatttggt ccctgcaaca taccatcagt agctatatct gtataaataa
     4681 tctctgcaat accaatgttt tgaatatcct ttataaattc aatggcattt atgtcaataa
     4741 tttttttcca gccgtgtgtt gcaacatgta aatcccttgc atcaacacct gctataatat
     4801 actctttaaa cctttgaatc atttctctaa acgattcttc aataaggatg gtacccatta
     4861 ttatacgctc tattccctca tcaagataca ttttaatagc agcttcggta cggataccac
     4921 caccaatttc aataggaata gataccgatt ttgcaatctc ctttacaata tgcatgttga
     4981 caggctgccc ttcaaatgct ccatcaaggt caacaacatg aataagctgt gctccagcat
     5041 cttcaaattg ccgggcgata gttaccgggt catacgaata caccttttcc tgcaatgggt
     5101 caccctgcac caggcgcaca catctgcctt cttttaaatc gattgccggg attacgagca
     5161 taccatcctc acaaagtttt caatcatggt gagtccatac cggtggcttt tttccggatg
     5221 aaattgcgtg gcaatacaat ttcccctccc cacaatgctg gggaatgtca ccccatagcc
     5281 agtttgaccc attatccagg aactatcgcc aataaccgga tagtaggtat gaataaaata
     5341 aaaataacta ttttgcggta taccattaac cactggatgt tcttttatta gttcaacctg
     5401 attccagccc atatgaggca cctttagatc atccgggaat cgtatcacag taccgggtat
     5461 aatgtcaagc cccttatggt tgccaaactc ttcactgctg gtaaacaaaa gctgatagcc
     5521 aagacatatg ccaaaaaagt aaccgccatt acctataaac cgtaccaatg gttcaatcca
     5581 gcctgattgt gtcagatttt ccattgcctg accaaaagca ccatctcctg gcattatcaa
     5641 cgcttttgat gaggctatgc tcctggggtc agagctaacc tgtacatcac tggtatattt
     5701 ttctacagct tttacaaccg agcgcaggtt gcccatgcca taatcaatga cggtaatcat
     5761 aacactcctt ttgttgatgg tataccggtg cgaccactta agctgtaagc ccggtatagt
     5821 gccaatcctg ctgatttaaa gattgattcg tgtatatgat gacggttgtc gccatacatc
     5881 tgttgtatat gcagtgtaat cagggcatta tgtgcaaacg attgaaaaaa ttccagtgta
     5941 agttcttcct ggtattgccc tattgccccg gtaagtttat cgcctttata tacaaagcac
     6001 cccctccctg atatatcaac aacagcagca gtgcaagcct catccatagg gatgactgcc
     6061 tgcccaaacc tgctaatccc gcttttatcg cccaatgctt tattgaacgc ctttcccatg
     6121 catatcccaa tatcctcaac agtatgatga tcatcaacct ccagatcacc ccttgcattg
     6181 agctgtatat tcatccttcc atgcgtaaca aacaatgtga gcatatgatt aaaaaaacca
     6241 attcctgtag tgatggtgct tacatcacca ctatcaagta tacattttaa tgttatgtca
     6301 gtttcatttg ttttacgttc tatatgtgca cttcgttcca ttgcattaaa ctccttgaaa
     6361 atagaatctt tcccccatca aaattcatat cctaaactaa agagtatctc tttttcacca
     6421 aactcaccat aataatatgg ccatgcataa tcaagtttta aaataactat tgggtatacg
     6481 gtaagcctaa atccaaatcc catgtccatt ttaaaatctt caaattgatc tgtttcatta
     6541 ttaaaaaaag tatacgaatt atcccatgct gaacctgcgt caacaaataa aacccctcca
     6601 atatttccaa tctttaaaaa gagtggccag cccatgttta ttgattcaat gaatgtaaac
     6661 ctgaactcag cgttgaacaa aaaaacattg ctaccaccat attctacaaa cggatgtccg
     6721 cgaagggtat taaatccacc aatataatat ttaaaatact ctttctcact tcccataatc
     6781 ttgccaccaa caccgcggag cgctataaca tactgtttat caatgaaaaa ataatgccgc
     6841 aggtcaatat ctatgctggt aaatgaataa tcctgccccg tcaggttaaa cacctgggtc
     6901 aacattatct gcccacgagt gccccgcaat ggcgctaaaa aaccccacaa aacattatcg
     6961 taattgtatg atacagaaat tgaatttaaa ttgccataaa catccggacg ttcatcacta
     7021 tagccgtaat cctgttcata gcggcttgag gaaacacggg tgctgaaacg cgaaaattta
     7081 gtaaaaggat agcttgcaat cacataggca ccgtaatgat ccattgaaag cgttgaccag
     7141 tatgcattat gaataatatc gttaaggttt gccagcgtaa atatgccata gggacttttt
     7201 tgtctgaaac agccaaaggt ccaatcccat cgatatcgta aataaagata ttgcacatcg
     7261 taattcataa agttgttgct ctcatcatcc cgcaaaaaat cagttgttac aataatccgg
     7321 ttatcgccca gataatcgct gaagctcatc tgcgcaaaac cagtaaagcc atagccaacc
     7381 gttccgccaa gcccaacaaa aacccagtca gatgaaatcc ttgtttcata gttgtccaac
     7441 acagcttttg atagtggaaa atagtttggg ggcagcatca ccggtatatg ctcactgtta
     7501 aacagcgggg tttctgttac aggttttatt tcatttttta taaagatatc gtatccacca
     7561 ttttgatatg aaacaaacgc aaatgcattg ccctccggaa aaagacgcgg ataaaatata
     7621 ccggttgttc ccgtagtaat ttttatttcc tcgcccgttg caatagtata acgataaaca
     7681 ttatagattc catccctgtt tgagatatat acaatagtat tgccatcccc tgcaacatca
     7741 acctgtaaat tttttgactg agccttcact ataacctgtc gctcacctgt tagcacatta
     7801 tgtcttataa ttgcatagtc ggtacctgta taatcattag ttggataatt tgatgaatag
     7861 taaataaaag catttttttt atcaagcctg ggataccgtt ccgtatacac atcattggtt
     7921 atctgctgta acattgtatc gttgagcctg tagatataca catcactctg cgtattggtc
     7981 tgccctataa agcttatata gttgccatca ggtgataagg caggatcttt aatcgaacga
     8041 aatggcagta ctatcttttt gataattgta ccgtttcgac tatccagtat aaagataaca
     8101 tccctgttgt tggattgtga aacaaacaca atgcctttgc catccggtgt ccatgacaga
     8161 tagttatcca gcaaatgcat cccttcaaac tgagaggttt cattcccttt taataacaca
     8221 cgggcttttg cttcagcctc tttttcgcca atggtaataa ccgaaattga agcatagata
     8281 tccctgttat cgatataggc aattttttta ccatcaggcg atatggcagg gcatgtattg
     8341 aacactgaac gcgttttttt atgatcggtt accctttcac cttcctcttc atcaaaattt
     8401 ctgccaacca tcaaagggta atatcttttt ttataaaagt gcctgaattc cttatcaata
     8461 gcctcaagat ccttttttgt atgtaccttt aatgcctttt caaagttacc catatcgcgt
     8521 atgtcacgca aaaattcgcc aatttttccc ttgccataca ccgtttcaaa aaagtaaaag
     8581 aatgcctgcc ccactttata gtagtagtac aaattacctg tacgataggt agtaaagtcc
     8641 atcaggctta catactgatc attaaaaaag aaatcacgca tggtcatatc ggcagtttca
     8701 tcaaatccaa ttgatagata ctctgacata ccttcggtaa accacaatgg cacagcaccc
     8761 atactcatca tcgactgtag tttaccggaa ctatcgtgca taagaatatt gaactgaaac
     8821 gcatgaacaa gctcatgaac cagaacatgc tgatattcat cgtacgaacc actaaacggt
     8881 atcaccaccc tgtttttaaa tgcttcagta aatccgccag taccctcgcc aatgatctgc
     8941 atcaggatat tattttcctg aaaatctatg tgcgaaggga atacaatgat tggtaccggt
     9001 tcattgagct ggtgttgcag tgtatcagca agatatccgt atgccctttc agcgattgtt
     9061 gctgtcgttg cagccagcct ttcaattcca tagggataat acacattgaa atgtattgtc
     9121 cttaaaatat gccaggtaaa tacctcaacc gtaaccttgt ttttgccaaa tgggtataca
     9181 aataaaggaa cggttatgat gacacatagt attgcactat aacaaatttt atgtttgaaa
     9241 aattttttca acactatgga tgtagccttt aaagcttttt tgtgttcgat agttactgta
     9301 tatcagctat tattatgaaa ccatgctcca cactgtatta gtgttttcac tcacattagc
     9361 aagtaaaatt tttgttgcca ataaatatta tttcagtata ttggacagta agcctgcggt
     9421 gttcgtgtat ggtatgtcat attgtagttc caatactact gagcagggaa tctctaatct
     9481 gcggattgat atcacgcttc attatgaagt agatagatgt ccgtatgcga gaatccacct
     9541 ttacaaaggg agctcaaata ttcataccac tgcggcacac gcgggttaat ttaatggtat
     9601 gcggccagta tggaaataat tacagtattt ggagtacggt ggcgggtagc tcccctgtgg
     9661 gaggtgcttg taacattatt tgttatcctt gtaatcatag gtttttttgc actgctgcga
     9721 tataaccaat atctcaaatt aaaaaaatca agtattgaac aactttttat ctataaagcg
     9781 cgccagaggg gattaacagg gtatcagatt cgtattcttc aacacatcat atcactgtct
     9841 aaattaagca ctcccgaaat tatttttaac aatgtaacca tttttgaaac aggtctggta
     9901 aaatttttac agtatataaa aaagtcaaga gatacattca atgaagattt aaaagatatt
     9961 tttaaagata tagttataac ctatgaaagg atatataacc cggtggaata tcaggagcaa
    10021 ttaaattcac cggatgaatt gactacgaat accatggtat gcggctatag tgcaacagga
    10081 agggcattta tagctaagat aacacagatt actggagatg ccatccattg taaatatttt
    10141 cgtaaacctg aggatatccc tgctgacatc attgcagagc aagtaacaat tttctttttt
    10201 agaaatggag atgccgaata ttcttttgat tcaaaaatca tagccattca ggaaaatacc
    10261 atagtattac aaaagccaca agcatttaaa agaggcaaag aggtacatca tccatatatt
    10321 caaacactta ttccctgcac tgttcagctc aaaaacgatg aaagcaaaaa tagtgaagaa
    10381 gtaataatac aacatgatga aaccgtttca ataaatggaa ctatagagcg cttaaatgag
    10441 tatgaagctg taatcagggt tgagaaacgg ctggaatatc aaaaagaata tatactattt
    10501 tttacacttg aaaattttaa aattgaagta ctcagcacca ttattgcctc acggacaata
    10561 accagagaaa aaattattta ctataccttc aaatttgttc agatgtcaga agcggcaaaa
    10621 aatgtaatct ctaaatatgt aaaagagcgg ctgtaaacac cgctctttta agagtaaaat
    10681 tctctatcag tatctaccgt ttccccttca atgcgagcaa attcctttaa taattctttc
    10741 tgacgaggtg ttaatttttt tggtaccttg atattaattt ttaccaattg atcacccttg
    10801 ccgtacgaac ccagatatgg cattccattg cctttaagtc tgaatatttg accattttca
    10861 gtccctgctg gtattttcat ttttgctttt ttattgtata ttgttggaac ctctatctca
    10921 cctcccagac aggctatagt aaacggtata tcaacaacac aaatcagatc atttccatga
    10981 cgttcaaaca acggatgttt ctgaatatgt actacaacat ataaatcgcc tgccgaacca
    11041 cctccgggac cctgctcgcc ttccccggaa atttttaaac gagatccgga ctcaacacct
    11101 gcaggaatct ttaccgtaat tttgcgatgt ttttcttcta accctgtacc tctacatgcc
    11161 ctgcatggtg aggttataat ttttccttcc cctctgcatt tataacatgt ctgtgttact
    11221 gaaaaaaatc cctgcgtctg acgtatttgc cctgtcccat tacaaacagg acataccgtt
    11281 ggttttgaac ctgatgccga accggtacca ccacaactac tgcatacctc atggcgtgga
    11341 atttcgatct ggacctcttt accattggct gcatcgtcca gtgttattga caaatcatat
    11401 tgaatatctc ttccccgacg cgtacgggat gatcgtgttc gtgtgccaga accagaacca
    11461 gcaccaaaaa agctttcaaa tatatcacct atatcaaaat caccaaagat atccgaaaaa
    11521 tcggtatatg ctcctctgcc aaagccttca aatcctgcaa caccttcatg cccaaactta
    11581 tcatactgtg cccggcgctt tggatcgcgt aatacctcat atgcttcagt agcttcttta
    11641 aatttctctt ctgcctcttt acttccttta ttgcgatcag ggtgaaattt taaagcaagc
    11701 tttctataag cttgctttat ctcatcttct gttgcatttt taggaacacc cagaacttca
    11761 taataatctc gcttcgtcgt agccaaggtc atcaccccta tttaaaaaga ttacaataat
    11821 tgtgcgccaa tggcaaccat tgtcgcacaa ttatttacta ttatagttta ttttttctta
    11881 tcttcatcta ccacttcata atctgcatca tatacctttt caccaccctg agtgctttcc
    11941 tcagttgtgc cagaagtatc cgattgttgc tgtgattgag attgagcata ttgttcagca
    12001 ccctgcttat aaagcttttc tgcaaatgaa tgggatgctc tttccagttt agctttcgca
    12061 tcacgtatca cattaatatc acttgattcc attgctttcc tgagattttc aatttcagat
    12121 tctattgaag atttttccgc agcatctatc ttcccttcat tttcctttat cagtttttca
    12181 agtgaataga ttaaagcatc cgcctcatta cgagcttcta caagttgacg cgcctttcta
    12241 tcctcttcag catgttgttc ggcatcctta accatcttct tgatttcttc ttcattcaaa
    12301 ccacttgagg cttcaatcct tattttctgc tcttttccag taccaagatc ttttgccgat
    12361 acatgcaaaa tcccgttagc atctatgtca aatgttacct caatttgcgg cacgccacgg
    12421 ggtgcaggtg gaataccaat gaggtcaaat cgacctatgg ttctgttctg cgaagctaat
    12481 tcgcgttcac cctgtaacac atggattgac acagccgtct gattgtcagc agcagttgaa
    12541 aatatctgac tctttctggt tggtatagtt gtgttacgtt caatgagttt ggtcattaca
    12601 ccacccaatg tctctatacc aagtgacagc ggtgtaacat ccagcagcaa cacatcatga
    12661 acttcgccag ctaaaactcc accctgaatt gcagcgccca cagcaacaac ctcatccgga
    12721 tttacacctt tatgaggttc tttaccaaac acccgtcgta ccaattcctg aacagcaggg
    12781 attcgtattg atccgcccac taagattact tcatcgatat caccaggggt aagtcctgcg
    12841 tcttccagtg cttttataca cggttctttt gtagattcaa tgagatcttc taccaatcct
    12901 tcaaattttg agcgtgtcag agtcataata agatgtttag gaccattctg atcagctgta
    12961 atgaatggta tattaatctc agtttgcaat ttattggata gctctatctt agctttttct
    13021 gcagattctt tcagtctttg taacgccatc ttatctgtat gcagatcaat tccagtttgt
    13081 tttttaaatt ctgatattaa ccactccatt atacgctgat caaaatcatc gccaccaaga
    13141 tgcgtgttcc cattggtaga ttttacctca aaaaccccct cgcccagttc aagtattgat
    13201 atatcaaatg ttccaccacc taaatcatac actgcaatct tgtgattgcg ctctttctta
    13261 tcaagcccat acgccaatgc tgcagcagtt ggttcgttta ttattcgttc aacggttaaa
    13321 ccagctatcc tgcctgcatc ctttgttgcc tgcctttgtg aatcattgaa atatgcaggt
    13381 acagtaatga ctgcacgagt tactttttca cccagataat cctcagcact ctgcttcatc
    13441 ttttgtaaaa ttcgtgctga aatttcctga ggtgtaaatt cccctgcaat tgttttaatc
    13501 ttcacaccac ctttaccgtc atcagcaacg gtaaaaggaa ccatctttat ttcgttttgc
    13561 acttctccat aggaccgccc cataaaccgt ttaatggaac gtattgtatt ttcaggattc
    13621 gttatcattt gatttttagc aacctgccca actaaacgtt cgcctttgtc cgtaaatgca
    13681 acaaccgatg gagttgtcct ttgcccttct gaattgggta taacaactgg ttcaccacca
    13741 accataactg caacacatga atttgtggtt ccaaggtcaa tccctattat tttgctcata
    13801 gtaattctcc ttttctttta aagtattgaa tataattgaa tgatagtatg tatcattgtt
    13861 cattttcatc cctgttaatt ttgtgacgca gcagtatttt cctcagttgc attgtttgca
    13921 ttgtttgcat tgtcatcttc taccttctgc tttgccgggc gcgtcacctt cacccgtgca
    13981 gcacgtacaa catatttatc cagtttaaag cctttctgat atactttggt aatagtatcc
    14041 tttacaacat caggactttc atcaatttca accgcttcat tatactcagg atcaaacgct
    14101 tgtcctaaac tattaatctc ttcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnntcaaaat cagcctgtct gcgtaccagc agatctttca gagcatctaa ctcttcattt
    14281 ttcttattaa gttcttcctg taacttacgc aactggtcct ccttttgctg tattagctca
    14341 ctgctcgatt caacctgatc attaccagca gattgattca atgaactatc accattctcc
    14401 tgaactttta ttttttcttc attcattgtc tgtattttct cttgatttat tgcatcatga
    14461 atgttatcat cttccctttt gtgcctttta tcatgtttgt tactcataac gccccctgcc
    14521 atacaatggt aatgtaacaa ttcataacta tattattttg tcatttgtgt taaaaaattt
    14581 gaaactactt tgccggtata atctacaagc ggaactactt tttcataatc catgcgcgtt
    14641 ggaccaataa caccaacaac acctaccgga ctattaccaa ttttatatga tgaagcaata
    14701 atgctacaac atgggattgc aatatctttt atttcatctc cgataagcgt aaagacacct
    14761 tccttctgcc ttagatcttc cataatctta cgcagggtgt ctttctgctc aatgaaacgt
    14821 actatttcat gtaacttttc agcttcaacg atatcgggta tatgcagcag attctcaatc
    14881 ccatcaatgt aaatatcagg ctcctgttca tccttcagcg ctaattcggt tatatcaagt
    14941 gctacatcac ggactgatgt ttcgacatcg gttacggtac gcaactgatt aataagactc
    15001 tgtcgtattt caaccagcga gtatcctgaa agctcagccg tcaaaaaacg tgaatattta
    15061 tacaaatcat cctgatttat agatgatgac agtttaacct ttttatgaat aacaattcca
    15121 gtacgggtta ctatgatgaa caacacatca ttagacgtta gcgagattaa ctcaatatgc
    15181 tttactacgg taaaatcagg ttttggcatc agggcaatac ctgtgtattt tgaaataagc
    15241 gatagcatct taacgataga cataaatatt ttatccagtt caatctcacg agccaacatc
    15301 tcttcgcgta cttctatatc agtttttgcc actgtttcat aggtatccag caatgaatct
    15361 acataaaagc ggtaaccctt atcagttggt attctgcctg ctgaggtatg cggatgggaa
    15421 agatatccca tctgctccag gtcaaacata atattgcgca tggttgccgg tgataatgaa
    15481 aatgaatact tttgaacaaa agaacgagaa cctaccggct ttcctgttgc tatatactca
    15541 tatacaattg ccttaagaac ctgtgcttcg cgatcattta actgttttgt atgcatgata
    15601 gcaacctcta tatacatatt agcactcaat ctatatgagt gctaataata tactaaaaaa
    15661 aatagcgccc gtcaagtcat tttccttaaa aatagtggaa taaagatgat attttcatat
    15721 ctttagtgta tagacataca taaaaccgct tatttataca atcatcctat gaacaatcaa
    15781 aagaagtgtt gttagcaata ttttgtatac acagggattt aacaaattaa cgttttcaga
    15841 atacccttat tttttctttt ttttctcttc ttcctctttc tcgtccgctt ccaatcgcaa
    15901 gcgtaacgct tcaatagtat tttttaattt ttcagcttct tcagattcac caaataatat
    15961 atgaatcctg cgcagcgctg ttaaaagttc tattgacttc aggatatctt cggatgattt
    16021 tgtggaaagc tcatatttat tgcgatattg cctggcagct tcggataagc tttgcttgca
    16081 taacttcaac atatcctgtc gttcatagta aaacgctttc cgcgggtctc tatatttttg
    16141 caattcactg aagtttatca tgttttttgt tattacggca accctgccct gtaactcaac
    16201 aaacgaccac ttccatttag aactttcgcc caatgcatcc attatttttt gcaacaatac
    16261 atgaatttct ttaacaagag ctaatgtttg ttgaggattg agtttattaa tagcggcaag
    16321 gtattcttca ttttctttta acatacgatc aatatcctta ccaactatct cctcaaaaag
    16381 ctgtaaaacc ctgtagaatt ctttacgcgc atcatttaaa aaacgctcat tttttacttt
    16441 tagcatattc accgatactg tgttcatgtt cagcagcgta tagatatact tgatatagag
    16501 caaaatcaac tcaagattta tatacccctt gataccgggc atctttttct ttttttgctc
    16561 agcttctttt atctgtttgg ttaactcttc taactttgct ctatagcctt taacaaaatc
    16621 attataggca accttttcgg ctttcgtaac tggcatgata gtttccctta tgcataagta
    16681 tcgatggttg atttgtttac cttctctggc tgctctggtt tctccttttc cacagtattt
    16741 tcctgctgtc cctgattatt gacagccggt cctgctggca ctacatcagc acctgaaatt
    16801 cgcataattt ccatattttc catccctccc tgaagatata ataataaaca ttttctacac
    16861 tctatgcacc gttaccatgg tatgttcact atcatagtgt aataattaat atatgcgata
    16921 gctcctgtaa aatagcaaga aaaaaattaa aaattactat cgctcatatt aatgttatta
    16981 ttttccttag cactataaaa ttatttcatt gaaattgcac cgctgttagt ggaaattctt
    17041 tagcggtata aatcttcata atcttcaact taccttactg tcaattttta ttttttgatc
    17101 ttcaagtatt ttttc
//
LOCUS       DAOOJH010000048        16628 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3353_length_16628_cov_9.193326, whole genome shotgun sequence.
ACCESSION   DAOOJH010000048 DAOOJH010000000
VERSION     DAOOJH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16628
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_16628_cov_9.193326"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLJ75_07600"
     CDS             complement(<1..241)
                     /locus_tag="PLJ75_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05115.1"
                     /translation="MAHGERINKRKENKRRHIVDIFRHHTTLSKAEVKRYSGYSMDTV
                     MNSFKILKNSGIIVPTEGKQKEKGRKAFFYTLDHEK"
     gene            587..>1316
                     /locus_tag="PLJ75_07605"
     CDS             587..>1316
                     /locus_tag="PLJ75_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05116.1"
                     /translation="MNTIIAMGTILFVILAILFIIIILLQFDKSRGMGILGRYRNTAS
                     CLSTADITITITMVMVAFVMLGSPGLSTFELHSAYSYEKDALQQEKTGAVIAQKGKAL
                     QQVQPDGSQIDDNSMLKVEHDESSFLVYYKNRCILKHSANNPCIAVGNGTAEYAMSKG
                     SFKIKEKIANKINLKKFTIKENSDTITINFDDTVTIAINAKDNCTISFQCKPDYNRLW
                     LKLEAYKKEHIYGCGEQFSKLDLKN"
     assembly_gap    1317..1416
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1417..2604
                     /locus_tag="PLJ75_07610"
     CDS             <1417..2604
                     /locus_tag="PLJ75_07610"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPD05117.1"
                     /translation="AVWVQDWVGKRITWFGKQLFWDWRYSQELYPDLPALIKELSHKN
                     IKFLGYINCFLTTDGDLYKEASQKGYLVKDKTGAVRHIVTTAFPAGVIDLTNPHACRW
                     IKDVIKKNMIDLGLNGWMADFGEYLETDTQLYSGESAELFHNTYSVQWAKVNYEAIKE
                     AGKLGDIVFFTRAGYTGTSQYSPLIWAGDQLVNWSKNDGLASVIPAGISLGFCGVGYY
                     HSDIGGYTTLLWIKRSKELFMRWAELSAFTPVMRTHEGNRPESNWQFNSDKETLQHLA
                     KMTRIHTALKPYFEKCKDEYHTNGLPLIRHPYIHYPDDEKLHLFQYQYLLGRDLLVAP
                     VYSKGKKKWEVYLPHDNWIHVWSGKEFSGGTVKIDAPLGQPPVFYRKDSKLSDLFMKL
                     KDI"
     gene            2905..3912
                     /locus_tag="PLJ75_07615"
     CDS             2905..3912
                     /locus_tag="PLJ75_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05118.1"
                     /translation="MKLSYKISQINDTIYRISLPLPGNPPYINVYCFKGEVNTLFDTG
                     ISWTWRLLQKALHEIGLQFKDIHQIIPSHGHVDHYGAANAIVKRSNNNIQVIAHNADA
                     MRIEKGVYANPLVMLRFMQSMGVPKALIVPLAILDVAFKSMGHNVTITHKIIDEVYTL
                     RVGDYNAKLILTPGHSKGQMCLFIEKESLLFSGDHIVPGVKPIILAGFEPNTSFPVSQ
                     SHAIFHTALKKITKLHPRVTFPAHGEPFDGKLDRLINTYHKIYQLRKQRIFSILLNND
                     YTVYSLARKVFKSLKGINLVLDMYLSLSETYTHLEAMCIEGEVIKYSFDNVIYYTAAL
                     Y"
     gene            4375..5574
                     /locus_tag="PLJ75_07620"
     CDS             4375..5574
                     /locus_tag="PLJ75_07620"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPD05119.1"
                     /translation="MRLISIAIPIIFIFVISCFNTVESKNNNTGLTVSFTGDVIMHGP
                     VKSFAFRNNIIDIKTKRTINNGGFDILFEHIKPEFENSDYVVTNMEFPVAPPFTSKPF
                     IFNCTPAVLDAFVSNHINIVTLANNHILDQQYEGLLSTIKILESKNINYIGAGTLINT
                     TKDSINGIVVGDSIKIGIIAYTGVFNYELSRKLKKDIYINDFYSINKVLNDIARIKQK
                     CDFLVMVAHIGEEYKTQPAKKDRDIIHTYCEAGVDCVIGHHPHVIQPVEQFKTNDGRF
                     CTIFYSLGNFIANQSSVHIDAISSVECSTRETIIVQLTVTKNTENNHHYTVIPVMIIN
                     IPDTESKYKYGKRIQPIAIMQYINLLKKENNNSEYINYLINRKNELIKHLFLYDNYQN
                     IKIKDNY"
     gene            complement(5580..6701)
                     /locus_tag="PLJ75_07625"
     CDS             complement(5580..6701)
                     /locus_tag="PLJ75_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD05120.1"
                     /translation="MSNGISTDNLDALINNCIQTLNINKTKFDNLPDYERALAYYSIY
                     HTNKIHLLLVSNTYKGLATTFTQTGKVFEELAYHYFDTLPTMLTTIHCLELIKSIADD
                     NKKIFLLNHIIDAKKPFAFCLTEENAGSDITAITTVATKNNNQYIINGTKDIVINSGI
                     ASFYIVFARSGKKGRAGLNAFLIPENAKGLCTNKLMLDSFYNSVLGTVTLENVTIDEE
                     YQIGEDGSGYFLLTEMLDKGRPLVAACCVGGSQRAFDIILEHTKKRKQFSKTLYNFQG
                     INFTIAEFETRIHAARLLYQDACKKIDNGEPFSKESSIAKYFASRLFLDITTFGMDVL
                     GYRSIFNASEIKHLNNCAHTMMFIDGTENVQKMIIASLL"
     gene            complement(6694..7038)
                     /locus_tag="PLJ75_07630"
     CDS             complement(6694..7038)
                     /locus_tag="PLJ75_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD05121.1"
                     /translation="MAFKKIGKKIQIAREEKGITQIELSKMLGITQAALSNYELGKRR
                     LYLQQIEEIAKHLEKPVSYFLDEESSSNIDDELSDITINSITDKIKNLTHYEINELNQ
                     FIDYLLWRRNHE"
     gene            7439..8686
                     /locus_tag="PLJ75_07635"
     CDS             7439..8686
                     /locus_tag="PLJ75_07635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD05122.1"
                     /translation="MFDYIMNDEQLKLRDEARDFAKWVPREMILDMDAEKIHFPKEFL
                     KEAGKRNLLGIRLPEQYGGRGLNWVDDCIVAEEIGVASYSLACLWGVGADIVCEAIVE
                     FGSEELKQEIVVPLLKGDVYAAECLTEPRGGSDFFGASTVAKKDGSDWIITGQKRFIV
                     GAEGADWFMVYAVTNPEGKAHQRLTCFMVPRTKGVETKYIYGLMGVRGGGAGRLILNE
                     VRVPERYALNGINGGFDVFVRNMLPERLGTAAMTIGSVRPAVEIATRYTSMRKAFGQP
                     IAYFEAVGFKVADSVMLLDATRSLVYSTALAIDSGKVHPGRVRRMVSESKKFVTESAW
                     TIANNCMQVMGGIGYTNVYPLERIVRDIRLSMIWVGTNEIMDLIIQNEWYKEYFKVIS
                     KLNLRDVEQDAPGADQVEEKVYE"
     gene            complement(8711..10312)
                     /locus_tag="PLJ75_07640"
     CDS             complement(8711..10312)
                     /locus_tag="PLJ75_07640"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05123.1"
                     /translation="MKIEFVGGARTVTGSSFIVKNNNFTVMIDCGMFQGTRELRERNY
                     LNLIYAPSQIDSLLLTHAHIDHSGLIPKLVKEGFTQPIYATKATCELCSIMLPDSAHI
                     QEQDAEIINKKRKKLGREYIEPLYTSEDAERCMQYFNPVRYHQTVSIHPDITVSFKDA
                     GHILGSSFIEMNIKDNGKQNAIVFSGDIGQKNQAIIRDPELPDYADILLIESTYGDRL
                     HKSKEDTYNELKKVILESYNKHGNIIIPAFAIERTQELLYTIAQLVKKGEIPPIPVYL
                     DSPLATSATHIFLNNQDCFDDAINNLLRSGDSPLDFPNLHFVKTVEESKWLNKNAKGA
                     IIISASGMCTAGRVKHHLLNNLYKPESAVVFVGYQAEGTLGRRIVDGAHMVKIYGENV
                     AVRAKIYTIGGFSAHADKEGLLDWMSTIKNPKLKVFVVHGEENASALLAEEIRNRFGY
                     TVYVPHWGEIVDLETMKSEYAKYSPFEIADNVESELEHVEKLVQTLKARYKKAKEEGR
                     RINWNQIENDISDIRELVSMINDEL"
     gene            complement(10346..11014)
                     /locus_tag="PLJ75_07645"
     CDS             complement(10346..11014)
                     /locus_tag="PLJ75_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD05124.1"
                     /translation="MDIIKQYGFGSSTITVLKNISITINKGEIVSIVGPSGAGKSTLL
                     NIIGCLDTFQQGYVQILDQDISPFSYDELATFRNKHIGFIFQMHNLMPEFTALENTMM
                     PLLIRRVSKKEAKHKALELLEHFKIVDRAGHKPAELSGGECQRVAVARALITQPDIIL
                     ADEPTGNLDSKNSDILIELLFKNCKDKGSTAVVVTHDQSIAAKTQRIITLIDGMIQSE
                     TMNL"
     gene            complement(11071..12375)
                     /locus_tag="PLJ75_07650"
     CDS             complement(11071..12375)
                     /locus_tag="PLJ75_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD05125.1"
                     /translation="MRLYELFIGWRYLKAKKSQGFISFNTFLSLFIVAIGVFVIIVVM
                     SVMTGFQSQIRDKILDVDAHITVSRFFVDNNSEGLPDYKELSNKIKTIKGIRSIEPFV
                     QGQGLVRVKSYIFPVAIRGYGIPGMVPDDIKKFVTAGDKEFSKPTTIAIGEEMAFNYN
                     LRLGDYLEIIVPKGRLTAKEGITPGIGKYRIATYFKTGYYDFDTKLIIMTIPQTQQLY
                     GIGNIVSGLALKLDDIYDMDKIASTVQGLIGYDYQVTTAEQQNQNLFYALRLEKLIMT
                     IILFLVIISASFTIMGTLVMVVMEKRKSIGILKTMGAHPYSIMTIFVLEGFFIGLIGA
                     IVGVITGLVTAINLESIIHWIERTINGVMSWIFITFDLGIFYPVSLVPKNVYYIDSLP
                     TQIQPEFVMIVAILAIFIATVAAILPSWYASRLHPVESIRYE"
     gene            complement(12483..13400)
                     /locus_tag="PLJ75_07655"
     CDS             complement(12483..13400)
                     /locus_tag="PLJ75_07655"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HPD05126.1"
                     /translation="MKQFCHYGIAIMLLFCLYSIAFANEDHDFYITTALGCGFSQGVL
                     GEYVYDNGRKVSQLHWNIDHLYYYFYEVALYYGWFTTGLSLQNSYDHSIGTINDSDWT
                     DNNNPYVKTNFSHHDNYLEYMHNYNVYCGFLVVNNKDFQLIMKAGYIYKNIKMSARDG
                     YVEYPPGSPKVSMYGIGIIYQQEYSIPYIAIEAMYTMYNLSLSGGLSCGIFADADDVD
                     NHVKRDIDFHDDFDGILYLCGKAGIQYSFTHFIIGGKVIYEYVPVTKGDEYYINNSNG
                     SKSPVYNNCGGCKLAVMSIELFISIKVEL"
     gene            complement(13464..14123)
                     /locus_tag="PLJ75_07660"
     CDS             complement(13464..14123)
                     /locus_tag="PLJ75_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05127.1"
                     /translation="MMRKGIVSFVCIMIVAMSNIFYTTYLFAVDIALNYSPCSLLADY
                     KENSRTINIKDDSVDYFDKWGYLSKASLLFLFPNNDKEHPYMTGYFIDILHKSVSIEP
                     VDIKYFEKGGDIHIVSGIAGIMFRGPVKTTNIYFDFGFGIGYTAIFKDSYGAHSYGND
                     TDYTFKLGAGYKVNLSHSIACNFGVDWIAPGLSSYHKLNHGGYVNLYYGGIYPYIGVA
                     F"
     gene            complement(14297..15190)
                     /locus_tag="PLJ75_07665"
     CDS             complement(14297..15190)
                     /locus_tag="PLJ75_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05128.1"
                     /translation="MNESDRELIDGQLTIIEDELSAFMTRNFRAECYDLSSKSAMAAE
                     IMVNGNISQAMYTLAEKDVLIQFSFHEMLYDNIKLNNGQDKTIKSNKYIVYEFKNNST
                     NQERAWENKEISVREVSNVNMTDTNTTDTDIENNQIELSLPMEDSNLAVEFETELYQN
                     LETKQGVEVNGNEDTNVISLDNGVEITVEYETISNTEKGLVKKIKKAAHKVKHSVSNA
                     WDKYGPQIYGTLCIISGVVILAEYYGAAFMAGITSGFAAIPLIYGAHPFATAIGTGLI
                     TSGCVSIIKNKYYIGFSYSYK"
     gene            complement(15517..>16628)
                     /locus_tag="PLJ75_07670"
     CDS             complement(15517..>16628)
                     /locus_tag="PLJ75_07670"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05129.1"
                     /translation="LNVPQNAIVDSLQGRGIIISAGQLNALVTQGHSIFHNEKEDILE
                     TGLKIVPYISVDDTGARHQGHNGYCTHIGNDVFSYFKSTNSKSRVNFLRILRGRHNDY
                     MISQESIDYMMMHRIPPPLVNRFRGKLNKRIATEEEWESLLHIIGIHREHDKRIITEA
                     ALLGSILSHGIKRDMVIVSDDAGQFDIVVLLHALCWLHAERGLSTIVPINDYHKKIIE
                     DMRRKVWELYHNVQEYKQYPTKEKMLLLESQFDTLYRQETEFEDIKKALESMRQNKKE
                     LLLVLQRPEIPLHNNSSENAIRVYVLKRKIHGGTRSELGRECRDTFLSLKKTCRKLDI
                     SFYEYILDRLTGRYRIPPLCQLLRERILTPLSFPA"
BASE COUNT         5396 a         2854 c         2731 g         5547 t
ORIGIN      
        1 gtttttcatg atctaacgta taaaaaaaag ctttcctgcc tttttctttt tgtttgcctt
       61 cggtaggtac tattatacct gaatttttta agattttaaa tgagttcatg acggtatcca
      121 tggaataacc ggaataacgt ttcacttcag cttttgaaag agtagtgtgg tggcggaata
      181 tatctactat atgtctacgt ttattttctt ttcttttatt tattctttca ccatgagcca
      241 tacggtaacc tcgtatttga ttagtttcgt tatataaatc aatatcgata gtatcatttt
      301 cttaaaaaat aacatataac attattgcaa atatttttta ctttttcaat tattttttgc
      361 aatttaaaat gacgaggatt attaattagg taaagcttta tccattcgta tttcaatatt
      421 ttacggttat acgttgcaaa catttaacaa agaatagact tttttcatta cacagatatt
      481 agtcaataat ataatctttt cttgacataa atattatttt agcataatct tacagttata
      541 ctgaaaatat ttcattgtag caaaatctga ataatcaagg tgcagtatga acacaataat
      601 agcaatggga acgatacttt ttgttatact ggcgattctg tttatcatca ttatattatt
      661 gcaatttgat aaaagtagag gcatgggcat attgggtagg tacagaaata ctgcatcttg
      721 tttatccact gctgacatta ccatcaccat taccatggtg atggttgcat ttgttatgct
      781 gggttcaccg gggctatcaa catttgaact gcatagtgca tattcatacg aaaaagatgc
      841 gttgcagcaa gaaaagacag gcgctgtgat agcgcaaaaa ggtaaagcgt tacaacaagt
      901 gcaacctgac gggtcacaga ttgatgacaa ttccatgcta aaggtggaac atgatgaaag
      961 ctcattttta gtttattata aaaaccgttg tattttgaaa cattctgcta acaatccatg
     1021 tattgctgtt ggcaatggta ctgcagagta tgccatgagt aagggttctt tcaaaataaa
     1081 ggaaaagatt gccaataaaa taaatcttaa aaaatttacc ataaaagaaa attctgatac
     1141 gataaccata aattttgatg ataccgttac cattgcaata aatgcaaaag ataactgtac
     1201 tatttcattt caatgtaaac cagattataa cagattgtgg ttaaagcttg aggcatataa
     1261 aaaagaacac atctatggtt gtggtgaaca attttcaaaa ttggatttaa aaaatannnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngctg tatgggtgca ggattgggtt
     1441 ggcaaacgga taacatggtt tgggaagcag ttattctggg actggcgata ttcacaagaa
     1501 ttatatcctg acctgccggc tcttattaag gaactatcgc acaaaaatat aaaattttta
     1561 ggctatataa attgtttttt aaccacggat ggcgatttat ataaagaagc atcccaaaaa
     1621 ggatacctgg taaaggacaa aactggcgca gtacggcata ttgtaaccac agcattcccg
     1681 gcaggggtga ttgacttaac caatccgcat gcatgcagat ggattaaaga tgtaataaag
     1741 aaaaatatga ttgatttagg tttaaacggc tggatggcag attttggcga atatcttgaa
     1801 actgatacac agctttattc aggtgagtcg gcagaactat ttcataatac atattcagta
     1861 cagtgggcaa aagtaaatta tgaagctata aaggaagccg gcaagttggg tgatattgta
     1921 ttttttacgc gtgctggtta tactggtact tcgcaataca gtccattaat ctgggctggc
     1981 gatcagcttg ttaactggag taaaaacgat gggcttgctt cggttatacc ggcaggaatt
     2041 tctctgggtt tttgtggagt agggtactac cattcggata ttggtggtta tacaacattg
     2101 ctatggatta agcgaagcaa agaactattt atgcggtggg ctgaattgag tgcatttacg
     2161 ccagtaatgc gaacacatga aggtaaccgc ccggaatcta actggcagtt taacagcgat
     2221 aaagaaaccc tgcagcattt agctaaaatg accagaattc atacagcact caaaccctat
     2281 tttgaaaaat gcaaggatga ataccatact aatggcttgc cattgatcag gcatccgtat
     2341 atccattatc ctgatgacga gaaactacat ctattccaat atcagtattt gttaggcagg
     2401 gatttactgg tagctccagt gtacagtaaa gggaaaaaga agtgggaagt atatcttccg
     2461 catgacaact ggattcatgt ttggagcgga aaagaatttt ctggtggaac cgtaaaaata
     2521 gatgcaccgt tagggcagcc accggtattt taccggaaag attcaaaatt gagtgattta
     2581 tttatgaagt taaaggatat ataagccatg ctaataattc tttaacaact gcaaggtatg
     2641 attctgcatc tctatcacat agtattgcca taccaggttt tgattcatca acattttatt
     2701 tataaaatca ttgacaacta tcggagataa acaattattt tattcaatac ataatgatgc
     2761 cacttataaa taatttttga tgtatttctc ttagtataaa cagtggaaat tttgtacttt
     2821 ttattataat aaaataaata gtataaatag tcaaaaatga tttttcagtg tgaagattat
     2881 tattttatga aaacatggta aaatttgaaa ctatcatata aaatatcaca gattaatgat
     2941 accatataca gaatttccct gccacttccc ggtaatccac cttacattaa tgtttattgt
     3001 tttaaaggcg aagtaaacac cctttttgat accggaatat catggacatg gagattattg
     3061 caaaaagctt tgcatgagat tggattgcag tttaaggata ttcatcagat tataccatca
     3121 catggtcatg tggatcatta tggtgcagca aatgcaatag tgaaaagaag caataataat
     3181 attcaggtga tagcccataa tgctgacgca atgcggatag aaaaaggagt ttatgccaat
     3241 ccattggtaa tgttgcgatt tatgcaatct atgggtgtac caaaagcgtt gattgtgcca
     3301 ttagcaatcc ttgatgttgc atttaaatct atgggtcata acgttaccat cacccataaa
     3361 ataattgatg aagtttatac attacgagta ggagattata atgcaaaatt gattttaaca
     3421 ccaggacatt caaaagggca gatgtgttta tttattgaaa aggagagttt gctattttct
     3481 ggagatcata ttgttcctgg agtaaaacct attattttag cgggctttga accaaataca
     3541 tcattccccg tttcacagag tcatgctatt tttcatactg cattgaaaaa aattacaaag
     3601 ctacatcccc gtgtaacatt tcctgcacac ggtgaaccct ttgatggaaa acttgacagg
     3661 ttaatcaaca cctatcacaa gatataccaa ttacggaagc aacgaatatt ttccatacta
     3721 ttgaataatg attacacagt ctattcgctt gcacgaaagg ttttcaaaag tcttaaggga
     3781 ataaatttag tgctcgatat gtatctttca ctttcagaaa catacactca tttagaggca
     3841 atgtgcattg aaggtgaggt catcaaatat tcatttgaca atgtaatcta ttatactgca
     3901 gctttatatt aattaaggtt aaactactat actatctggt tccatgtttc atggtatatc
     3961 atgttcattt tttatgcgat agttactgct tatgaatatt ttcgtaaata acgtattaac
     4021 agcagcatta caatcaatgc caaaaaattt atttctgttg atacctatga cacttaaatt
     4081 atacggagag caggttcacg gtatgctgtc ggtttgttac atttggtggt gatagttact
     4141 attatagagt aattggaatg tgtttaatgt ttttatgaat acaatttcta atgtgacata
     4201 atatatctta tcagaagtaa tgaaagttca aatggaagtt cagtatctat ttttattaga
     4261 ttacgataaa tgaatagaca aatttaaacc tgtataaaat atcttttaaa agatattgca
     4321 taatggatat gtactttata atgagccaat aaaccatagg gactatcata tagtatgaga
     4381 ttaataagca ttgcaatccc aataatattt atatttgtta tttcctgttt taatacggta
     4441 gaatctaaaa acaataacac cgggttaaca gtatcattta caggagatgt tattatgcat
     4501 ggtcctgtaa agtcgtttgc tttcagaaat aatattattg atataaaaac aaaacgaact
     4561 atcaataatg gtggttttga tatactattt gaacatataa aaccagaatt tgaaaattca
     4621 gattatgttg ttacaaatat ggagtttccc gttgctccac cgtttacaag caaacctttt
     4681 atattcaatt gcactccagc agttttagat gcatttgtga gcaatcatat aaatattgtg
     4741 acacttgcaa ataaccatat tcttgatcaa caatacgaag gtttactgtc caccataaaa
     4801 atacttgaat caaaaaatat taattatatt ggtgcaggta cactgataaa cacaacaaaa
     4861 gattcaataa atggcattgt agttggtgat agtatcaaaa taggcattat tgcctataca
     4921 ggagttttca actatgaact atcacgaaaa ctaaaaaaag atatttatat aaatgatttt
     4981 tatagcataa acaaagtatt gaatgatatt gctcgtatta aacaaaaatg tgattttctt
     5041 gtaatggtgg ctcatattgg tgaagaatat aaaacacaac ctgcaaaaaa agatagggat
     5101 attatacaca cctattgtga ggcaggcgta gattgtgtta taggacacca tccgcatgtg
     5161 attcaacctg ttgaacagtt taaaacaaat gatggacgtt tttgtaccat attttacagc
     5221 cttggtaatt ttatagccaa ccagtcttca gtccatattg atgccataag cagtgttgaa
     5281 tgcagtacac gtgaaacaat tattgtacag cttactgtaa caaagaatac tgaaaacaat
     5341 caccactata cagtaattcc cgtaatgata atcaacattc ctgacactga atcaaaatat
     5401 aaatatggga agagaattca acccatcgct attatgcagt atataaacct gcttaaaaaa
     5461 gaaaacaaca acagtgaata tataaactat ttaataaaca gaaagaatga gttgataaaa
     5521 catctgtttt tatatgacaa ctatcaaaac ataaaaatta aggataacta ttaatacatt
     5581 tatagtagcg aggcaatgat cattttctgg acattttccg tgccatcaat aaacatcatg
     5641 gtatgcgcac agttgttaag atgttttatc tcgcttgcat tgaaaatgga acggtagcct
     5701 aaaacatcca taccaaaagt tgtaatatct aaaaataatc gtgatgcaaa atattttgct
     5761 atagaggatt ctttgctaaa aggttcacca ttatcaattt ttttacatgc atcctgatat
     5821 aataatcgtg ctgcatggat acgggtttca aattcggcaa ttgtaaaatt tattccctga
     5881 aaattgtaaa gagtttttga aaattgtttt cgtttctttg tatgttctaa tattatatca
     5941 aaggctcttt gtgatccacc aacacaacaa gcagctacca atggccttcc cttatcaagc
     6001 atttcagtta acaaaaaata accacttcca tcttctccta tttgatactc ttcatctata
     6061 gttacgtttt ccagggttac cgttccaagc acactattat aaaaactatc taacattaat
     6121 ttgtttgtgc ataatccttt agcattttcc ggaattaaaa atgcatttaa acctgccctg
     6181 ccctttttac cgctccgtgc aaaaacaata taaaacgatg caatgccgct gtttatgaca
     6241 atgtcctttg taccattaat aatatattgg ttattatttt ttgttgcaac tgttgttata
     6301 gctgtaatat ccgagccagc attttcttcg gttaaacaaa atgcaaaagg cttttttgca
     6361 tcgatgatat gattaagtaa aaatattttc ttattatcat ccgcaataga ttttattaat
     6421 tccagacaat gaatcgtagt taacatggtt ggcaaagtat caaaataatg atacgcaagt
     6481 tcttcaaata cttttcccgt ttgagtaaat gttgtagcaa gacctttgta tgtattactt
     6541 accagcagca gatgtatttt attagtatgg tatattgaat aatatgccaa agccctttca
     6601 taatcgggta aattatcaaa ttttgtttta ttaatattta atgtttgtat acaattattt
     6661 attaatgcat caagattatc agttgatatc ccgttactca tgatttcttc tccatagcaa
     6721 ataatcaata aattgattta gttcatttat ttcataatgt gtaagatttt tgattttatc
     6781 agtgatagag ttaatggtaa tatcagatag ttcatcatca atgtttgaag aagattcttc
     6841 atcaaggaaa tacgatacag gtttttcaag atgttttgca atctcttcta tttgctgtaa
     6901 atacaatctg cgtttgccta attcatagtt tgataatgca gcctgtgtaa tgccaagcat
     6961 ctttgacaat tcaatctgcg ttatgccctt ctcttcacga gctatttgaa tctttttgcc
     7021 aatcttttta aatgccataa catatactca ttttttatag taccatcgat gtcatagaaa
     7081 tagtgaaaaa tataagtttt tatacttctc tgaaaacata ccatgatagt ctcgcatctt
     7141 acatttagca gacaggtagt atactgtgtt acgcgattat taatgcatat ttactttttt
     7201 atgacaataa aaaatttttt ttgatatttg tattgacagt ataacagcta taacactaat
     7261 ataacaatat gttatatttc ataggtaact atcgtacaaa aaattgttga atatagtttt
     7321 gagtaatagc atcataatta tcagcatata ttgttatcga tagttgttat cgcaataatg
     7381 aactgaacct gataaatgaa tgtagcaatg aataccatat atatgtgagg aggcatatat
     7441 gtttgattat atcatgaacg atgaacaatt gaagttgagg gatgaagctc gtgattttgc
     7501 aaaatgggta cctcgtgaaa tgattttaga catggatgca gaaaaaatac atttcccaaa
     7561 agaattttta aaagaggctg gaaaaaggaa tttgctgggc atacgattgc cagaacaata
     7621 tggcggcaga ggactaaatt gggtagatga ttgtattgtt gcagaagaga ttggcgttgc
     7681 cagttattca ttagcttgtt tatggggcgt tggtgctgat atagtgtgtg aagcaatagt
     7741 tgaatttgga agcgaagaat taaaacagga aatcgttgta ccgttattaa aaggtgatgt
     7801 ttatgctgca gaatgcctga cagaaccacg cggcggttca gatttttttg gagcatcaac
     7861 agttgccaag aaagatggca gtgattggat tattaccggt caaaaacgat ttattgttgg
     7921 agctgaaggt gctgattggt ttatggtata cgcggtaacc aatcctgaag ggaaggctca
     7981 tcagcgttta acctgcttta tggtgccacg aacaaaaggt gttgaaacaa aatatatcta
     8041 cggacttatg ggagttcgtg ggggtggtgc tggtcgctta attttaaacg aggtacgagt
     8101 ccctgaacgg tatgctttga atggtattaa tggtggtttt gatgtttttg tcagaaatat
     8161 gttgcccgaa cgtttaggta ccgctgcaat gactattggc agtgtgcgcc ctgcagttga
     8221 gatagcaacc cgatatacct ctatgcgtaa agcatttggt caacccatag catattttga
     8281 agcagttggc tttaaagtgg cagatagcgt catgttactg gatgctaccc gatcactggt
     8341 atacagtaca gcgctggcta ttgattcagg caaagtacat ccaggaagag tgcgaaggat
     8401 ggtgtccgaa tccaaaaagt ttgtaaccga atccgcatgg actatcgcca ataactgcat
     8461 gcaggtaatg ggtggtattg gctataccaa tgtttatcca cttgaacgta ttgtaaggga
     8521 tatcaggtta tccatgatat gggttggtac caatgagatt atggatttaa tcattcagaa
     8581 tgaatggtat aaagaatact ttaaggtaat ctcaaaatta aatttgcgag atgttgaaca
     8641 ggatgctcct ggtgcagatc aggttgaaga aaaggtttat gagtgatagt tattaaagat
     8701 tgggcatgcg ctacagttca tcgttaatca ttgatacaag ctcgcgtata tcagatatgt
     8761 cattttcaat ttgattccag ttgatgcgcc tgccctcttc ttttgctttt ttatatcgtg
     8821 cttttaaagt ttgcaccaat ttttcaacat gctcaagctc agattcaaca ttatcagcaa
     8881 tttcaaatgg actatatttt gcatattctg atttcattgt ttcaagatca actatctcac
     8941 cccaatgagg cacataaact gtatagccaa atctgttacg tatttcttcc gctaacaatg
     9001 ctgatgcatt ttcttcgcca tggacaacaa agacttttaa tttaggattt ttgatagtac
     9061 tcatccaatc aagcagtcct tctttatcag catgagcaga aaatccacca atagtataaa
     9121 ttttggctct aacagcaaca ttttcgccat atattttgac catgtgtgca ccgtcaacaa
     9181 ttcgtcttcc cagagtacct tctgcttgat atccaacaaa tacaaccgct gactcgggtt
     9241 tatataaatt attcaacaga tgatgcttta ctctccctgc agtacacatc ccacttgcag
     9301 atatgattat ggcacctttt gcattcttgt ttaaccattt tgactcttcc actgttttaa
     9361 caaaatgaag attaggaaaa tccagaggac tatcgcccga acgcaataag ttatttatag
     9421 catcatcaaa acaatcctgg ttattcaaaa aaatatgagt agctgatgtt gctaaaggtg
     9481 aatctaaata aacaggtatt ggggggattt cccccttttt cacaagctgt gctatggtgt
     9541 ataataattc ctgggtacgt tcaatagcaa atgctggaat aattatatta ccatgtttat
     9601 tatatgattc aagtataact ttttttaatt cattataggt atcttctttg cttttatgaa
     9661 gtctgtcacc ataggttgat tctataagca atatatcagc atagtcggga agttcaggat
     9721 cacgaattat tgcctgattt ttttggccaa tatcaccaga aaatactata gcattttgtt
     9781 tgccattatc tttgatattc atctctataa acgatgaacc caatatatga ccagcatcct
     9841 taaaacttac tgtaatatca ggatgaattg ataccgtttg atgatatctc acaggattaa
     9901 agtattgcat acaacgctct gcatcttcag aggtgtatag tggttcaata tattcgcgtc
     9961 ccaatttttt tcgcttttta ttaatgattt cagcatcctg ctcctgaata tgggcactat
    10021 cgggcaacat aatactacaa agttcacagg ttgcctttgt ggcatatatt ggctgagtaa
    10081 aaccttcttt tactaatttt ggaataagtc cactgtggtc aatatgtgca tgggttaaaa
    10141 gtaaactgtc tatctgtgaa ggtgcataga tgagattaag ataatttcgt tcccttagct
    10201 cacgtgttcc ctgaaacata ccgcaatcaa tcataacggt aaaattatta ttcttaacaa
    10261 taaatgatga accagttacc gtgcgtgctc caccaacaaa ttcaattttc ataacaccat
    10321 aattcctttt tcattttatt attaattata aattcattgt ttcactctgt atcatcccat
    10381 ctattaatgt aatgattctt tgtgtttttg ctgctatgct ctgatcgtga gtaacaacca
    10441 ctgctgttga gcctttatcc ttacaatttt taaataaaag ttcaattaaa atatccgagt
    10501 ttttactgtc cagattacct gttggctcat cagccagtat aatatcaggt tgcgttatga
    10561 gtgctcgtgc aacagcaaca cgctgacatt caccacccga caactcagca ggtttatgcc
    10621 ctgctctatc aactattttg aagtgctcta acaactcaag tgctttgtgt tttgcttcct
    10681 tttttgatac tcttcgtatt aaaagaggca tcatggtatt ttccagtgcg gtaaattcgg
    10741 gcatcaggtt gtgcatctgg aagataaaac caatatgctt atttctaaat gttgccagct
    10801 catcatacga aaatggtgaa atatcctgat ctaatatctg aacatatccc tgctggaagg
    10861 tatcaaggca cccaataata tttaacaggg ttgattttcc tgcacctgat ggaccaacta
    10921 tcgaaactat ctcaccttta ttaatggtta tactaatatt ttttaacact gtaatagtgc
    10981 tgctgccaaa tccatattgt ttaataatat ccatgccttt aatggcacta ttgtgtacgg
    11041 tatcatccat agggttatcc atagcaataa ttattcatag cgtattgatt caacaggatg
    11101 caatcgtgat gcataccatg atggcagaat ggcagccaca gtggcaataa agattgccag
    11161 tatagcaaca atcataacaa attcaggttg tatttgcgtg ggaagcgaat caatataata
    11221 tacattcttt ggcaccagcg aaacaggata aaaaatacct aaatcaaaag ttataaatat
    11281 ccatgacatt acaccattaa tagttctctc aatccagtgt atgattgatt ccaaattgat
    11341 ggcggtaaca agcccggtaa taacgccaac aattgcacca atgagcccga tgaaaaaacc
    11401 ttcaagcaca aagatggtca tgatggaata tggatgcgcc cccattgttt ttaatatgcc
    11461 aatagattta cgtttttcca ttaccaccat gacaagtgta cccattatgg taaatgaagc
    11521 tgagattatc actaaaaata aaataatagt cataattaac ttttcaagac gcagtgcata
    11581 gaataaattt tgattttgtt gttcagcagt tgttacctga taatcatagc caatgagtcc
    11641 ctgaacagtt gaagctattt tatccatatc atatatatca tcaagtttca aagcaagtcc
    11701 gcttacaata ttaccaatgc catagagctg ttgcgtttgc ggtattgtca tgatgataag
    11761 cttggtatca aaatcgtaat atcccgtttt gaaataagtt gcgatgcggt atttcccgat
    11821 gccgggcgtt atcccttctt ttgcggttaa ccttcctttg ggaacaataa tctctaaata
    11881 atcgcctaag cgcaaattat aattaaatgc catctcctcg ccaatagcta ttgtagtagg
    11941 tttactaaat tctttatcac cggcagtgac aaattttttt atatcatcag ggaccatacc
    12001 gggaatgcca taaccacgga ttgctacagg aaaaatatat gacttaacac gcactaaacc
    12061 ctgtccctgg acaaatggtt ctatagagcg tattcctttt attgttttta tcttgttcga
    12121 tagttcttta taatcgggta aaccttcgct attattatct acaaaaaagc gactcactgt
    12181 tatatgcgca tcaacatcca gaattttatc gcgaatctga ctctggaagc ccgtcattac
    12241 tgacataacc acgataatga caaatacgcc aatagcaact ataaacagcg ataaaaatgt
    12301 attaaaagag ataaatccct gagatttttt tgccttaaga taccgccatc ctatgaacag
    12361 ttcatataat cgcatagata ttccttgaaa aaaattataa cgaatgtatt aaattatagt
    12421 attttgtcaa atgtatttat ttatatgaag atttcagaaa atgtttatat aaaatgcagc
    12481 atttataatt ctacttttat tgaaataaaa agttcaatgc tcattaccgc aagtttacag
    12541 cctccacaat tattatatac tggagatttg cttccattgc tattattgat ataatattca
    12601 tcgcctttgg ttaccggtac atattcatat atcactttgc ctccaatgat aaaatgggta
    12661 aatgaatact gtatgccagc ttttccacaa agatagagta tcccatcaaa atcatcatgg
    12721 aaatcaatgt cgcgtttaac atgattgtca acatcatcag catcggcaaa tataccgcag
    12781 gatagtcctc cagatagtga taaattatac atagtataca tagcttctat tgcgatatag
    12841 ggaatggaat actcctgttg atagataata cctattccat acattgatac ctttggcgaa
    12901 ccgggtggat attctacata gccgtcacgt gccgacattt ttatattttt atagatgtat
    12961 ccagctttca ttataagttg aaaatcttta ttattaacta ctaaaaatcc acagtaaaca
    13021 ttataattat gcatatattc cagataattg tcatgatgcg aaaagttagt ttttacatat
    13081 ggattattat tatcagtcca atccgaatca tttattgttc caatagaatg gtcataacta
    13141 ttttgcaatg ataatccagt ggtaaaccag ccatagtata atgctacctc ataaaaatag
    13201 tagtaaaggt gatcgatatt ccaatgtaat tgacttactt ttctaccatt atcatacaca
    13261 tattcaccaa ggacaccttg tgaaaagcca catcctaatg ccgttgttat atagaaatca
    13321 tggtcttcat tagcaaaagc tatagaataa agacaaaata ataacattat agctatgcca
    13381 taatggcaga attgcttcat cactttattc accatgcaaa tttttacaaa tcaaaatttg
    13441 cgcatatact ataatagttt acattaaaat gcaacaccaa tataaggata aattcctcca
    13501 tagtataaat tcacataacc gccatgattt aatttatggt agctacttaa tccaggtgct
    13561 atccaatcaa caccaaaatt gcatgctata gaatgtgaaa ggttaacctt atagcctgcc
    13621 cctaatttga atgtgtagtc tgtatcattg ccataagaat gcgcaccgta actatcttta
    13681 aaaatagcag tatagcctat tccaaaacca aaatcaaaat aaatatttgt tgttttaact
    13741 ggacctcgga acataattcc agcaataccc gatacaatat ggatatcacc acctttttca
    13801 aaatatttta tatcaaccgg ttcaatggaa actgatttat gcaatatatc aataaaatat
    13861 cctgtcatat agggatgctc tttatcattg ttgggaaata aaaacaatag tgatgcttta
    13921 ctcaagtaac cccatttatc aaaataatct acactgtcat ctttaatgtt tatagttcgc
    13981 gaattttcct tataatcagc taaaagagag catgggctat aatttaatgc aatatcaaca
    14041 gcaaaaagat aagtagtata aaaaatgttt gacatagcta ctatcattat acaaacaaat
    14101 gatacaatac ctttcctcat catagtacac cccttttatt atcctttaca ttatataatc
    14161 ttactcaaaa tcattatata accttatata agagcaccaa acaaatagca aggtgccctg
    14221 atatctactt acactaaaac gattatttta ttattgtaaa aaattaaata tttagctcat
    14281 ctaatatgta ctattactat ttatagctat aactaaatcc tatatagtat ttattcttta
    14341 taatagatac acagcctgaa gtaatcaaac ctgtaccaat tgcagttgca aaaggatgag
    14401 ctccgtatat taaaggaatt gcggcaaaac cactagtaat accagccatg aaagctgcac
    14461 cataatattc agctaatata acaacacccg atataataca taatgttcca tatatctggg
    14521 gcccatattt gtcccatgca tttgatactg aatgcttaac cttgtgtgct gcttttttga
    14581 tttttttaac taatcctttc tctgtatttg atattgtttc atattctaca gttatttcca
    14641 cgccattgtc caatgatatt acattagtat cttcatttcc attaacttct acaccttgct
    14701 ttgtttctaa attttggtat aattctgttt caaattcaac tgctaaattt gaatcttcca
    14761 ttggtaatga taattctatt tgattatttt caatatctgt atctgttgta ttggtatctg
    14821 tcatattaac atttgatact tctctaacag atatttcttt attttcccaa gccctttctt
    14881 gatttgtgct attattttta aattcatata ctatgtattt attactcttt attgttttat
    14941 cttgtccatt atttaacttt atattatcat acaacatctc atgaaatgaa aactgaatca
    15001 aaacatcttt ttctgctaat gtatacattg cttgactaat atttccattt accataatct
    15061 cggctgccat agcagatttt gaagataagt cataacattc tgccctaaaa tttcttgtca
    15121 taaatgctga taattcatct tcaattattg ttaattggcc atcaataagt tctcggtcag
    15181 attcgttcat agatcgcatg gtatcttctg taacaattgc tttaaaataa taatcatatt
    15241 tctttgcaat gtcattggat gtaggagtat aattgctaca cgaaacaata taagtaaacg
    15301 atattatagt aataaataaa catgtttgta acaataattt ttcagttatg agggaatgtt
    15361 ttatttgaga tttgagattt gagatttgta atacatattt aatcctcgtg tttatataat
    15421 atattttttc tttaagttat aatagattat ttgtcaagta tatttttatt atttaataga
    15481 aaatatatag taactactca gaaactgtag gaaaaatcaa gcaggaaatg atagaggagt
    15541 aagaatacgc tcacggagca gttggcacag tgggggaata cgatacctcc cggtcagacg
    15601 atccaatata tattcgtaaa acgatatatc aagtttacgg caggttttct ttaaactcaa
    15661 aaatgtatct cgacattctc gtcctaattc gcttcgcgta ccaccgtgaa tttttcgttt
    15721 tagaacatat acccgtatag cgttttcact actattatta tgcaatggta tttcagggcg
    15781 ttgtaacact aaaagcagtt cctttttgtt ctgcctcatt gattcaagag ctttcttaat
    15841 atcttcaaat tcagtctctt gcctatagag agtatcaaat tgactctcta acaaaagcat
    15901 cttctctttt gtaggatatt gtttgtactc ttgtacattg tgatacaatt cccatacctt
    15961 cctgcgcata tcctcaatta tctttttatg atagtcattt atcggcacga tagttgaaag
    16021 tcctctttca gcatgaagcc agcataacgc atgtaacagt accactatgt caaattgacc
    16081 agcgtcatca ctgactatga ccatgtccct cttgatccca tgcgaaagta tgctcccgag
    16141 taacgctgct tccgtaataa tcctcttatc gtgctcacga tgtatgccaa taatgtgtaa
    16201 caacgattcc cattcttctt cagtagcaat ccgcttattc agtttccctc tgaaacgatt
    16261 gactaaaggg ggcggtattc tatgcatcat catatagtca attgactcct gactaatcat
    16321 ataatcgtta tgacgccccc tcagtattct gagaaaattt acccgacttt tgctgttggt
    16381 actcttgaaa taggaaaata cgtcattccc aatgtgcgta caatacccat tgtgcccttg
    16441 atgacgtgct cctgtatcat cgactgatat atagggaact atcttcaacc ctgtttctaa
    16501 tatatcttcc ttttcattat ggaaaatact atgcccctgt gttaccaggg cattcaattg
    16561 ccctgccgaa ataattatcc ccctcccctg caggctgtct actattgcat tctggggtac
    16621 atttaatt
//
LOCUS       DAOOJH010000049        16516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3379_length_16516_cov_10.474273, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000049 DAOOJH010000000
VERSION     DAOOJH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_16516_cov_10.474273"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLJ75_07675"
     CDS             <1..234
                     /locus_tag="PLJ75_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05130.1"
                     /translation="VKALAAKETVAKIIIGILAVLYVFLLYGSFNAYRAPGKVSQEML
                     EQTEKLSSEQQKALEQLQQLQKLLEKMQQEEKE"
     gene            complement(279..893)
                     /locus_tag="PLJ75_07680"
     CDS             complement(279..893)
                     /locus_tag="PLJ75_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015190871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPD05131.1"
                     /translation="MIHNNVKTDFISEEYSPQVDTSAYLHPMAAVIGHVIIGKRVMVA
                     PFAVVRGDEGQPIHVGDDSNIQDGVVIHALETEHNGHPINNNLVEVDSKKYAVYVGKR
                     VSLAHQSQIHGPAYVGDSTFIGMQSLIFKAKVGNNCVVEPKSLVLGVTIADRRYVPAG
                     SVIKTQGDADALPSIDDSYIFKSLNDDVIHVNVALADGYKVKKI"
     gene            1354..3648
                     /locus_tag="PLJ75_07685"
     CDS             1354..3648
                     /locus_tag="PLJ75_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD05132.1"
                     /translation="MFKQLLHRYSIHQVTLLSTESPLHQRIVRYLTRNRIEFSTITSF
                     NDSADTSLLILPLSMHTITPVSDILQQMLQLDHTPLLVFATDFPVTTRFKKRFSIAGI
                     IGILELTEHPRFITVESHTTQSNPTASLNALIYTTENLISRSIYKILSQNILYNALIK
                     LHPSIICITTIEGTIIYFRANREYLQRNNNIKKILYGKKIYDFATNPDSINNLFNLIN
                     KNIEQRETEITFCIDNNTFNLLVTGEKIQLYNDFNAYLLIGINITTQRKTEDLLISSE
                     LKYRTLVENLPDGLSLSKGEMLLYANPQFFKLFHYPPDTLMTDIDFHVNIHPEDWDTL
                     FPLLHQSLDGVTNLSIRGITNDEKQILLELHILSFQIDNSLYHQIYWQDITRTQKLLD
                     TILHSERLSAIGELASGITHEFNNILTSIQGYIQYTINNPDDAEANKKAFKVIEKMTE
                     QGHTILKNLSMLSRKDITNKERHSVAELLQDILKIQEKILTKDNITVVRKFFKDPVVY
                     VDPGLIQQVFLNILLNAVHAIRPKGKGTITITTDESNGNAIIKIHDTGTGIDPKIKDE
                     IFNPFFTTKQGGAYNIVGTGMGLSISKNIIEQHSGSIYFYSIPEKETEFIIELPKFSS
                     AYLPNEIKISCEPRDIANLSVLIVDDDEAIRDLFKTLLQSLGITTISFATNGYEGYSL
                     CKDSNFDIVFMDVSMPVLSGIDAYKMIKDEKPQQKVVFITGIFYEDQIKELVDKENAY
                     GYIKKPFDIKEIKALLYSIASCRR"
     gene            3673..4539
                     /gene="mtnP"
                     /locus_tag="PLJ75_07690"
     CDS             3673..4539
                     /gene="mtnP"
                     /locus_tag="PLJ75_07690"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HPD05133.1"
                     /translation="MKTTIDAGIIGGSGLYQIDGVTIVDEVSVKTPWGMPSDIITIAE
                     IDGVRAGFLPRHGRGHFILPHEINYRANIAALKMLGAGQIIAFSAVGSLKDRIKPLDF
                     VIPDQIIDRTKSRISTFFGDGIVAHIAFADPFCSRLHELILVAAQQLNIPMHTNEILI
                     CMEGPAFSTRAESHLYRSWQAGVINMSTLPEAKLAREAEMCYAVICMSTDYDCWKEDE
                     EHVTVDMVVNNLNKNAANAKALIKHLVPLLKQERTCACKEAIKYAIMTDSTKQPQKQK
                     KKLKAILPHYFS"
     gene            complement(4527..5960)
                     /locus_tag="PLJ75_07695"
     CDS             complement(4527..5960)
                     /locus_tag="PLJ75_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HPD05134.1"
                     /translation="MKHYKVTLAIPDTVAQEINTICTILLDNGFECYLVGGSVRDLIL
                     QIPVYDYDFATNARPEQVMKLFKKVVPTGIKHGTVTVLMSHAHYEITTYRSDGKYTDG
                     RHPDSIAFSDSLKEDVERRDFTINGLAYDVNKKVIIDYVGGMKDIKKKVIQTIGDPVQ
                     RFSEDGLRPYRACRLAAKLHFSINKKTLEAIPQMLDVASKISVERVRDEIKKMLEVDK
                     PSVGFEYMRVTGLLDLCLPELSQGFGMQQNKYHLYDIYYHSIYSCDAAPNDKPLIRLA
                     ALFHDIGKITTRREGHDGDYTFYNHEIIGAKIARRIMRRLKFSNDEIEYVTHLVENHM
                     FYYTQDWTDGAARRFIRKVGLHNLEDLFILRLADRVGSGMRDGLPEPIKYLKKHIEKV
                     IAKDNAFTVKDLDIDGYSIMDALHIKPGPMVGKILNELLEKVLDNPELNNYQTLLTLA
                     KEIYAIMERSDESSCHDSIQHQQHYEK"
     gene            complement(5957..8185)
                     /locus_tag="PLJ75_07700"
     CDS             complement(5957..8185)
                     /locus_tag="PLJ75_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD05135.1"
                     /translation="MKIFPIHELIYTVQKFPLLQSMTPCEIVRSLNFFEDVPFDCLNE
                     IVHNINIHQFNDNEIVARHGRYNEWFYIVLSGKVSVFMITPDYTKSELYSIEPDNFFG
                     EDIVISNEPRESTAIACGESILLAVDAQSIKKIIASSTRTNEMLNNAFLQRKMRNNLR
                     SIPIFTHLNDEIFNEIVDVVNVVQKKKGDVIFKQGDTGDALFMIRKGDVSVYRKMNKK
                     DELISLLAEGNFFGEMALVLGEPRNATVIANNDCELLKIEKKDFDTIVLRHKDLYNSI
                     HTVALERVSGHELVNATEAIVSKKLIELNRKLNKHIDVIAQCTFETPNGSALLATLPG
                     SRYPYVYPRDSACATRMLYRVAMSTLRSNDIAFRLLAGIARFICNCQRSDGYWGQRYG
                     LDTTEKSIYKQEDNVAHGVTILCRYCLAAKHKGHAPGDSEAYINAIYNGAMFAIKRYY
                     RNEIHLFYSTTSIHESAIEEGYSIWVNYAYWCMLSLIEKVAQEYDCVSRFERVIDLKE
                     SFGTIVNNVFTIDDRLIRRLKPDGEADLRPDITLMSPFFFGTGVMPHVFTEDAIFSNT
                     MKFVEDTLWDPDLGMLQRYLPFIEDPNTHVHAGNGPWVQYTAMLAQYNYFTGKVEKGD
                     RIIDSIDRYSADGYLCEHLTTPERFREFLTLEWLSGTDVSKEFAANIMVDGITYDLIV
                     EELNHMKNSYEQIKKKIEAGADNYLTFAMPLMWSHAEYAMALLLKTWRELLDSGVKNN
                     LL"
     gene            complement(8277..9938)
                     /locus_tag="PLJ75_07705"
     CDS             complement(8277..9938)
                     /locus_tag="PLJ75_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05136.1"
                     /translation="MIKKTIQHYIGIIALVIGCCLVTGLSYEIAYTIIFTPDGLDLFN
                     AKKVRLKNNTAGYTVATINYKESVNPLITDLLLSFDSDFPYLAKDDSGHYAIRYAQYK
                     YLQDAIEGEGCAQFFHRDHRVDIQTAKNNWLTNCDDLGSFTIEASIKLLQYSSAAIVL
                     SRTGFSQGKQGFEMLCVKNKLAVRFYKMFYKKDNTPIDVFLHRGPSLPLNAWQHISIS
                     FDRISGKLETWINGDVVETVYCTESGEPFVDVLVPKFNCHDLPIATVGKNFYGCIDKV
                     RISQRYIENLQKETAIAMSHYKPVSYNNRDTINREGIITSPVYQFPDFGTMVLLFHWQ
                     EQLPPSTYVWMEFRISDEMFKSDDMLPQWYRINNNQKNIYLKKVGDSYCRGKYYQWRA
                     HLISSPDGTNAPTIYAIELQYALDKAPQPPVMVAVDSVSDRVITLKWKKNVEYDILGY
                     KIYYGVKSGKYDGVITTIGNKRITNELSDNNGYISVTLTNEIIEENRNNDAKGFLQYP
                     VLKNNVIYYFAVTAYDSYKPDTLFNHESKPSNEVSARPEPGSDIQ"
     gene            complement(9942..10373)
                     /gene="rpiB"
                     /locus_tag="PLJ75_07710"
     CDS             complement(9942..10373)
                     /gene="rpiB"
                     /locus_tag="PLJ75_07710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPD05137.1"
                     /translation="MKIGIGNDHAGVDLKYSIIKTIPEHIFINAGTDSYESCDYPDYI
                     KLVAEMLQREEVDCGIAICGTGIGASIVANKHRGVRAALCTNEYMATMARKHNDANML
                     VLGARVVGIDLAIAIINAFLHATFEGGRHQQRLDKIQQIEH"
     gene            complement(10587..11972)
                     /gene="asnS"
                     /locus_tag="PLJ75_07715"
     CDS             complement(10587..11972)
                     /gene="asnS"
                     /locus_tag="PLJ75_07715"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006616883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPD05138.1"
                     /translation="MKRIRIVDCLQSDKSLESCTVAGWVRTKRESKENIFIEINDGSC
                     LTNMQVVVGFGDSCYETARQLHTGASVYITGKLVASPAAGQKWELKPDKLIVYGDADP
                     ESYPLQKKRHSFEFLREIAHLRPRTNTMGAVLRIRNQVSYAIHTFFQQKGFLYVHTPI
                     ITTSDCEGAGEMFQVTTLDLLHVPVQDGFIDYSQDFFGAKAALTVSGQLEGELLALAM
                     GDIYTFGPTFRAENSNTTRHLSEFWMVEPEMAFATLEDDCTLAEEFLKYIFTWVIDRC
                     HEDMVFFDERIKPGILHNLESIITSSFEIVTYTDAIAQLKQSPEPFEYAVKWGIDLQT
                     EHERWLTEKYYKKPIIVIDYPKEIKAFYMKRNDDNKTVKAMDVLVPGVGEIIGGSERE
                     DRYDVLLQRMQAMGLNPDDYWWYLDTRRYGSAPHAGFGLGFERAIQFITGMQNIRDVI
                     PFPRTPGSAKF"
     gene            complement(12112..12381)
                     /locus_tag="PLJ75_07720"
     CDS             complement(12112..12381)
                     /locus_tag="PLJ75_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05139.1"
                     /translation="MFRYLYGIHSGQRGCPDIRKLIIEPILPDKNAVLQPFVSPGLGF
                     EIDKKLLSKYGKRYFKMTELGLKIKVIREKGLKEALLLKKRKEGQ"
     gene            12693..13490
                     /locus_tag="PLJ75_07725"
     CDS             12693..13490
                     /locus_tag="PLJ75_07725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPD05140.1"
                     /translation="MRLLYITLLLLFTVSTLHAQDTVCVLVYHTFMGKKIHYDISLEE
                     FHKQLLELKDHGFTFVTFHEIKNGLVKGNKNILVTIDDGHRTALDAYYSVLKPMRIKP
                     LLGINTFIIGKKPYVLTWDELKQLSREGCYIASHGYFHLFINDKLYKNNYRYFKLEVF
                     ESKKMLEEKLCTPVDTFIYPFGVVSDVGKKALIEAGYNYAFTIKWGNTSLPITSNNLE
                     ISRYMYNQNWNAIARAIIYKHKNPSTPHKESEKILVSTMPRLKGTHQ"
     gene            complement(13456..14853)
                     /gene="gltA"
                     /locus_tag="PLJ75_07730"
     CDS             complement(13456..14853)
                     /gene="gltA"
                     /locus_tag="PLJ75_07730"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPD05141.1"
                     /translation="MADKIPRQKMPQQDAKNRIRNFNEVPLGYDEKTAMVEAARCLQC
                     KRPACVDGCPVNVNIPGFIKALKEGRPLEAFFTIKQTNSLPAVCGRVCPQEDQCEKLC
                     VLAKKGDAVAIGNLERYVADYEREKEHFVAQKSDSHTNKKVAIIGSGPAGLTAAGDLA
                     RIGYDVTIFEALHKAGGVLVYGIPEFRLPKDIVQYEIDALKAAGVTIGLSRVIGTAET
                     VNDLLSTGFDAVFVATGAGLPLFLDIPGENLQGVYSANEYLTRSNLMKAYKFPEYDTP
                     IVKGQKVAVFGGGNVAMDSARTALRLGSKEVYIVYRRSRTEMPARVEEVERAEEEGVI
                     FTLLTNPVQFIGDEKGRVKAVECLKMELGEPDASGRRSPIPVKGSEFTIAIDVAIIAI
                     GNGPNPLIPKTTPGMNITKRGNIIADPETMKTSKIGVFAGGDIVTGAATVIQAMGAGR
                     KAAKAIDEYLSTGAW"
     gene            complement(14843..15685)
                     /locus_tag="PLJ75_07735"
     CDS             complement(14843..15685)
                     /locus_tag="PLJ75_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPD05142.1"
                     /translation="MYTIVTNEKISDNVGKAIVEAPYIAKHRKAGQFVIVIVDEVGER
                     IPLTIADADSATGTITLYYQIVGTSTHKLSLKKKGEELFNVAGPLGHPTDIQCYGTCV
                     CVGGGIGIAAVYPIAVAMKDAGNYLISIIGARNKNLLILENELKAKSDEMIVTTDDGS
                     HGVKGFVTDELKTILDGGKVDIVVAVGPVPMMKAVSDMTKQYGVKTLVSLNSIMVDGT
                     GMCGGCRVTVDGKKQFTCVDGPEFDAHLVDFDELMKRLNTYKEQEKESFEKHKCKLEQ
                     YYGR"
     gene            16123..16434
                     /locus_tag="PLJ75_07740"
     CDS             16123..16434
                     /locus_tag="PLJ75_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05143.1"
                     /translation="MFVQTDKLAIEKGLANCKVLSIDSVKIRANASSKKDTSIDGLNL
                     LEDTIKEKLPRFMDDVDRDERRDEEIKALHRQEARVAEANKELRMLVLHLLIITKNLL
                     R"
BASE COUNT         5104 a         3526 c         2806 g         5080 t
ORIGIN      
        1 gtcaaagcgc ttgccgcaaa agaaactgta gcaaaaataa tcatagggat attggctgtc
       61 ctgtatgttt ttttactata cggttctttc aatgcatacc gagcccccgg gaaagttagt
      121 caggagatgt tagaacaaac agaaaagctt tcatcagaac aacaaaaagc attggaacag
      181 ctacagcaat tacaaaaatt actggaaaaa atgcagcaag aagaaaaaga ataattcata
      241 taacggttac atcctctgtg catagaggat gtaaccattt aaatcttctt taccttatag
      301 ccatctgcta atgctacatt cacatgaatt acatcatcat tgaggctttt aaaaatatag
      361 ctgtcatcaa tactaggtaa cgcatcagca tcaccctgtg tcttaataac agaacccgcc
      421 gggacatacc tcctatcagc tatggtcacc cccagaacca gtgatttagg ctctactaca
      481 caattgttcc ccacctttgc cttgaatatt aacgattgca tccctataaa ggtactatcg
      541 ccaacatacg ccgggccgtg aatctgtgac tgatgtgcaa gtgaaacacg tttcccaaca
      601 tatacagcat attttttact atctacctca actaaattgt tattgattgg atgcccatta
      661 tgctctgttt caagggcatg aatgacgacc ccatcctgaa tattggaatc atctcctaca
      721 tgaattggtt gcccttcatc accacgcact acagcaaatg gtgccaccat cacccgttta
      781 ccaatgataa catgtccaat gacggcagcc attggatgca aatatgccga ggtatccacc
      841 tgtggagaat attcttcact gatgaagtct gtttttacat tgttatgaat catagaccaa
      901 tcctccataa aaaatatatt gttttttgaa aggttatcga tattatattt ctgtcaagat
      961 taatcacaat gacatgaata aaaaagcgtt gatgaataac tattattgtt agtattgtgt
     1021 actcattgag aagttactca tggctggggt gtaacccata gatgatgtct atggaataat
     1081 actttaattg taaggcaaag ccgatagtta ttgcctatgg tatgcatcgt cgcagcaaag
     1141 aatctacttt ttcataacag agattctttg ggactgcgca catgcaaatg acaatgatcg
     1201 taggggattt tttagccgat gtactcccta tgcttgacaa tgcttggcaa tgattaacaa
     1261 tgctatggtg gtgttagtga tggcattact atatttaatt taagtgaacc tttatcaggt
     1321 ttttcatcat taaaaaatta ataacaaaat attatgttta aacaattact tcataggtac
     1381 tctatccatc aggtaactct gttatcaacg gagtcaccat tgcatcagcg tatagttcgc
     1441 tatttaaccc gaaatcgcat agaattttct acaattacat cgttcaatga tagtgctgac
     1501 acttctctgt taattttgcc gttgagcatg cataccataa cacctgtttc tgatatatta
     1561 caacaaatgt tgcaattgga tcatacaccg ctattggttt ttgctactga ttttcctgta
     1621 acaacgcgct tcaaaaaaag gttcagtata gctggtataa tcggcatact ggagctaacc
     1681 gaacatcctc gttttattac agtagaatcg cataccacac aatccaatcc aacggcttcg
     1741 ttaaatgcct taatatacac cacagaaaac cttatttcac gcagtatcta taaaatatta
     1801 tcacagaaca ttttatataa tgcattgatc aaattacacc cttctattat ctgtattact
     1861 accattgagg gaacaataat atattttaga gcaaatagag agtatttaca gcgcaataat
     1921 aatattaaaa agatactcta tggaaaaaaa atttatgatt ttgcaactaa tcctgattcc
     1981 ataaataacc tttttaacct cataaataaa aatattgagc agcgcgaaac tgaaataact
     2041 ttctgtattg ataacaatac attcaacctg cttgtcaccg gtgaaaaaat acaactctat
     2101 aatgatttta atgcctattt attaattggt ataaacatca caacgcaaag aaaaactgag
     2161 gacctgctta tttcatcaga attgaaatac cgtacgctcg tggaaaacct tcctgatggg
     2221 ttatccctgt ctaaaggtga aatgttactg tatgccaatc ctcagttttt taaattattt
     2281 cattatccac ccgataccct tatgactgat atcgatttcc atgtaaacat tcacccggaa
     2341 gactgggata cacttttccc attgctccat caatcacttg atggtgtaac aaatctttca
     2401 attagaggta ttactaacga tgaaaaacaa atactattag aattacatat attgagtttt
     2461 cagatagata attcattata tcatcaaata tactggcaag atataacccg gacacaaaag
     2521 ctattagata caatacttca ttctgagcgt ctttcagcca ttggcgaatt ggcaagtggt
     2581 atcactcatg aatttaataa catccttacc agcatacagg gctatataca atataccatt
     2641 aataatcctg atgatgctga agctaataaa aaagctttta aggttattga aaagatgacg
     2701 gagcagggac acaccatttt aaaaaatcta tcaatgctat cacgaaaaga tataaccaat
     2761 aaagaacgcc atagcgttgc agaattatta caggatatat taaagattca ggaaaaaatt
     2821 ttaaccaaag ataatataac agtagtaaga aaatttttta aagatccagt tgtctatgtt
     2881 gaccctggac ttatacagca ggtttttctc aatatcctgc tcaatgcagt tcatgccata
     2941 cgtccaaaag ggaaaggaac cattaccatt accactgatg aatcaaatgg taatgctata
     3001 attaaaattc acgataccgg taccgggatc gatccaaaaa tcaaagatga aatattcaat
     3061 ccttttttca ctaccaaaca gggtggagct tataatattg ttggcaccgg catgggatta
     3121 tcaatctcaa aaaacattat tgagcaacat agtggaagta tatattttta cagcatcccg
     3181 gaaaaagaaa ccgaatttat cattgagctc cccaaatttt cttcagcgta tcttcctaat
     3241 gaaattaaaa tttcatgtga acccagagat atagcaaatc tttcagtatt aattgttgac
     3301 gatgatgaag caatccgtga tctttttaaa acattattgc aatcactagg tataacaacc
     3361 atttctttcg caaccaacgg atatgaagga tattccttat gcaaggattc aaactttgat
     3421 attgttttca tggatgtatc catgccagtt ttatcaggaa ttgatgcata taagatgata
     3481 aaagatgaaa aacctcagca aaaagttgtt tttattaccg gtatatttta tgaagaccag
     3541 ataaaagagt tagttgacaa agaaaacgcc tatggctaca ttaaaaagcc gtttgacata
     3601 aaagaaataa aagcattact atattcaatc gcttcatgca ggcgatagtt attgaagaag
     3661 aggtgaaata caatgaaaac aacaattgat gctggcatta ttggcggttc aggattatat
     3721 cagattgatg gtgtaacgat agttgatgaa gtatcagtaa aaactccgtg gggaatgcct
     3781 tctgatataa taaccattgc cgaaattgat ggtgtcaggg caggtttttt accacgtcat
     3841 ggcagaggac attttattct gccccatgaa ataaactacc gggcaaacat tgcggcatta
     3901 aaaatgctgg gagcagggca aattattgca ttttcagcag ttgggagttt aaaagatcgg
     3961 attaaacctc ttgattttgt tatccctgat caaattattg acaggactaa atctagaatt
     4021 tccacttttt ttggggatgg cattgttgcc catattgcct ttgcagatcc tttttgcagc
     4081 agactgcatg aattaatttt agttgcagca caacagctca atatccccat gcataccaat
     4141 gaaatactta tctgcatgga aggacccgct ttttcaacac gtgcagaaag ccatctttac
     4201 cgttcatggc aggctggtgt catcaatatg agcacactac ccgaagcaaa attagcccgc
     4261 gaagcagaaa tgtgctacgc tgtcatctgc atgagtaccg actatgattg ctggaaagaa
     4321 gatgaagaac atgtcacggt tgatatggtg gtaaacaacc tcaataaaaa tgctgcaaac
     4381 gctaaggctt tgataaagca tcttgtgcca ttattgaaac aggaaaggac atgtgcctgt
     4441 aaggaagcta tcaaatatgc tattatgact gattctacaa agcagccaca aaaacagaag
     4501 aaaaaattaa aagctatcct gccacactat ttttcataat gttgttgatg ctgaatgcta
     4561 tcatggcatg agctttcatc agaacgttcc atgatagcat atatctcttt tgcaagtgtt
     4621 aaaagtgttt gataattgtt aagctcggga ttatcaagta ccttctccag tagctcattg
     4681 agtattttcc caaccattgg tcccggtttg atatgcaatg cgtccattat actataacca
     4741 tcaatatcca gatccttaac cgtgaatgca ttgtcttttg caataacctt ttcaatatgt
     4801 tttttaagat attttattgg ttcaggcaat ccatctcgca tgccactacc aactctatcc
     4861 gccaatcgca gtataaacaa atcctccaga ttgtgcagcc caactttcct gataaagcgc
     4921 cttgccgcac catcagtcca atcctgtgta tagtaaaaca tatgattttc tacaaggtga
     4981 gtaacatact ctatctcatc atttgaaaat ttaagcctgc gcataatcct gcgggcaatc
     5041 tttgcaccga tgatctcatg attgtaaaag gtataatcac cgtcatggcc ttccctgcgt
     5101 gttgtaatct tgcctatatc atggaataat gcagcaagcc gtattaaagg cttatcgtta
     5161 ggagcagcat cacatgagta aatgctatgg taatagatat catataaatg atatttattt
     5221 tgttgcatac caaatccttg cgatagttcc ggtaaacaca aatccagcaa accagtcact
     5281 cgcatatatt caaaacctac cgatggctta tctacctcca gcattttttt tatttcatct
     5341 cgtacacgct ccactgatat ctttgatgcc acatcaagca tctgtgggat tgcttccagt
     5401 gtttttttat taatgctgaa atgcaacttg gccgctaatc tgcacgctct atatggtctc
     5461 agcccatctt cactaaaacg ctgtaccggg tcaccaattg tctgaataac ctttttttta
     5521 atatccttca taccgccaac ataatcaata attacttttt tattgacatc atacgccaac
     5581 ccattgatag taaaatcacg gcgctcaaca tcctctttaa ggctatccga aaatgcaata
     5641 ctatcaggat gcctgccatc ggtatacttg ccatccgagc gatacgtggt aatctcatag
     5701 tgtgcatggc tcatcaaaac agtgacggta ccatgtttta ttcctgtagg gactactttt
     5761 ttaaatagtt tcattacctg ttcaggacga gcatttgtgg caaaatcata atcatacaca
     5821 ggaatttgta aaatcaaatc cctcactgaa ccaccaacca gataacactc aaatccatta
     5881 tccagaagaa tagtacaaat tgtattgatc tcctgtgcta ccgtatcagg gattgctaat
     5941 gttactttat agtgtttcat agcaaattgt tttttacccc actatctaaa agctcgcgcc
     6001 atgtctttaa caacagtgcc atggcatact ctgcatgtga ccacataagg ggcatggcaa
     6061 atgtcaggta attatcagca ccagcttcaa tcttcttttt tatctgttca tacgaatttt
     6121 tcatatggtt aagttcctca acaattaaat cataagtaat cccgtcaacc ataatattgg
     6181 ctgcaaattc tttactaaca tcagtcccgg acagccattc cagagtcaaa aattcacgga
     6241 atcgttccgg tgttgtcaga tgttcacata ggtatccatc agcactatac ctatctatac
     6301 tatcaataat tctatcccct ttttcaactt ttccggtgaa ataattatac tgtgccagca
     6361 tcgctgtata ttgtacccag ggaccatttc ccgcatgaac atgggtattg ggatcctcaa
     6421 taaaaggtaa ataacgctgt aacatcccta aatccgggtc ccacaatgta tcctcaacaa
     6481 acttcatagt atttgaaaaa attgcatctt cagtaaaaac atggggcata acaccggtac
     6541 caaagaaaaa tggactcata agggttatat caggacgcag atcagcttct ccatcaggtt
     6601 tcagccgtct gataagtcta tcatcaatag taaaaacatt gttaactatt gtaccaaaac
     6661 tttctttcag gtcaataacc ctttcaaacc gtgatacaca gtcatattcc tgcgccacct
     6721 tttctatcag tgacagcata caccagtagg cataattgac ccatatagaa tatccttctt
     6781 caatagccga ttcatgaatt gaggttgttg aatagaagag atgtatctca ttcctgtaat
     6841 aacgttttat agcaaacatc gctccattat agatggcatt gatataagcc tcactatcac
     6901 cgggtgcatg ccctttatgt ttagcagcca gacaatatcg gcacagtatg gtaacaccat
     6961 gagcaacatt atcttcctgt ttgtatatac ttttttcggt tgtatcaagt ccataacgct
     7021 gcccccaata tccatcactt cgttgacagt tgcaaataaa acgagcaatc ccggccagta
     7081 atctgaatgc aatatcatta gaacgcagtg tactcatagc aacccgatag agcatcctgg
     7141 ttgcgcaggc actatcgcgg ggataaacat agggatagcg tgagccgggt aatgttgcta
     7201 aaagtgcact cccatttggt gtttcaaagg tacactgtgc tatgacatca atatgcttat
     7261 tcagcttccg gttcaattca atgagttttt ttgaaactat tgcctcggtt gcattaacca
     7321 gttcatgtcc tgacaccctt tcaagtgcaa cagtatgaat actgttgtat aaatctttat
     7381 ggcgtaaaac aatggtatca aaatcttttt tttcaatttt taataattcg caatcattat
     7441 tagcgatgac ggtagcattt cgtggttcac ctaaaacaag tgccatttca ccaaaaaaat
     7501 taccctctgc aagcaatgat atcagttcat cttttttatt catttttctg tatacgctta
     7561 catctccttt tcgtatcata aacagggcat cccccgtatc accctgctta aagataacat
     7621 cgcctttttt tttctgcaca acattaacaa catctactat ttcattaaat atctcatcat
     7681 tgagatgagt aaatataggt attgatcgca ggttatttct catctttctt tgtaaaaatg
     7741 cattgttgag catttcattt gttcttgttg aagaagctat aatttttttt atggattgtg
     7801 catcaactgc aagcagtatg ctttctccac acgctatcgc tgtgctttca cgtggttcat
     7861 tactaataac tatgtcctca ccaaagaaat tatcaggctc tattgaatat aactctgact
     7921 ttgtataatc cggtgtaatc ataaaaaccg agaccttacc agaaagtaca atgtaaaacc
     7981 attcattata cctgccatgt cgtgcaacaa tctcattgtc attaaactga tggatgttga
     8041 tattatgaac tatctcattg agacaatcaa aaggaacatc ctcaaaaaag ttgagtgatc
     8101 gtactatctc acatggtgtc attgattgta aaagagggaa tttttgcact gtataaataa
     8161 gctcatggat gggaaatatc ttcatatagt atccttcctc aatcaataat gtgatacctt
     8221 aataatagat agagatatca ttaattgcaa gtataaatgt atacaatagt actgtactac
     8281 tgtatatctg aaccgggttc ggggcgggca cttacctcgt ttgatggctt tgactcatgg
     8341 ttgaatagtg tatcgggttt gtagctatca tatgcagtaa ctgcaaaata atagataacg
     8401 ttatttttta atacaggata ctgtaaaaat ccttttgcat cattattcct gttttcctca
     8461 atgatttcat tggttaatgt tacactgata taaccattgt tatccgatag ttcattggta
     8521 attcgtttgt tgccaatagt agtgataacc ccatcatact ttccactttt aacaccatag
     8581 taaattttat agcccaggat gtcgtactca acattcttct tccattttaa cgttatcacc
     8641 ctatcgctca cagaatcaac cgctaccatc acaggaggct gaggtgcttt atcaagggca
     8701 tactgtaatt ctatagcata gatggttggt gcatttgtac catctggcga agatatcaga
     8761 tgtgccctcc actgataata ttttcctcta cagtaactat cacccacctt ttttagatat
     8821 atatttttct ggttattgtt tatcctgtac cattgtggca gcatatcgtc gcttttaaac
     8881 atttcatcac tgatcctgaa ttccatccat acataggttg aagggggcaa ctgctcctgc
     8941 cagtgaaaca aaagcaccat cgtcccaaaa tccggaaatt ggtataccgg gcttgtaata
     9001 attccttcac gatttatggt atccctatta ttatagctta ccggtttgta gtgcgacatg
     9061 gctattgctg tttctttttg taaattttca atgtacctct gtgaaattct tactttgtca
     9121 atacacccat aaaagttctt ccccacagtg gcaatgggca gatcatgaca gttaaatttt
     9181 ggcacaagta catcaacaaa tggctcacca ctttcagtac aatagacagt ttctacaaca
     9241 tcgccattaa tccatgtttc aagcttcccg gagattctgt caaagcttat agaaatatgc
     9301 tgccatgcat tcaatggtaa tgatggtccc cggtgcaaaa aaacatcaat cggggtattg
     9361 tcctttttat agaacatttt ataaaatcgt acagcaagct tattttttac acaaagcatc
     9421 tcaaaaccct gtttaccctg tgaaaaacca gtacgcgata aaacaatagc agccgagcta
     9481 tactgtaaca attttatgga agcttcaatg gtaaatgaac ctaaatcatc gcaattggtc
     9541 aaccagttgt tttttgccgt ttgtatatca acacggtggt cacggtgaaa aaattgtgca
     9601 cacccttcac cttcaattgc atcctgtaaa tacttatact gagcatagcg tatagcgtaa
     9661 tgaccggaat catctttggc cagataagga aaatcgctgt caaatgaaag gagcaaatcg
     9721 gtaatcagtg gattaaccga ttctttataa ttgattgttg ctactgtata gccagcggta
     9781 ttatttttta atcgcacctt tttagcatta aaaagatcaa gcccatcagg cgtaaaaatt
     9841 attgtatacg ctatttcata agataggccg gtaacaaggc aacaacctat aacaagagct
     9901 attatcccta tatagtgttg tatagttttt tttatcatga gttagtgttc tatctgttgt
     9961 attttatcca accgttgctg gtgacggcca ccttcaaagg ttgcatgcaa aaatgcattg
    10021 atgatagcta tggctaaatc aataccaaca acacgtgctc ccagcacaag catattggca
    10081 tcattgtgtt tacgtgccat ggttgccata tactcattag tacaaagagc cgcacgcacc
    10141 cctctatgct tgttggcaac aatactggcg ccaattcccg taccgcagat ggcaattccg
    10201 caatcaacct cttctcgttg gagcatttca gcaaccagct tgatataatc ggggtaatcg
    10261 cacgattcat acgaatcggt ccctgcattg ataaaaatgt gttctggtat agtttttata
    10321 atactatatt ttaaatcaac acccgcgtga tcgttgccta tacctatttt catggtttat
    10381 cgctccaaaa atagaaaagt aatgatgcaa tatcaaatga caaatcctta caatgaaaaa
    10441 attctttgcc tgaactacgt ttagcacagg agctaccact tcagtataac tatcggcttc
    10501 gactcaacat tatactacta atacaatatc tactatacaa actatcatat caaataaatt
    10561 aactaactaa ctaactaact aactaactaa aattttgcgc ttcccggagt ccgtggaaac
    10621 gggatgacat ccctgatatt ctgcatccct gttataaact gtattgctct ttcaaaaccc
    10681 aatccaaatc cagcatgggg agccgaacca tatcgtcgcg tatccagata ccaccagtaa
    10741 tcatcaggat taagccccat agcctgcatg cgctgtagca atacatcata tcggtcttcg
    10801 cgctcactgc caccaataat ctcgccaacc ccgggtacta aaacatccat tgcctttaca
    10861 gttttattat cgtcattccg tttcatataa aaagctttta tctccttggg ataatctata
    10921 acaatgattg gctttttata atatttttcg gttagccacc gttcatgttc ggtctggagg
    10981 tctatccccc attttactgc atattcaaat ggttctggcg attgctttaa ttgcgcaata
    11041 gcatcggtat aggtaactat ttcaaatgaa ctggtgataa tcgattctaa attgtgtagt
    11101 atcccgggtt ttatccgctc atcaaaaaaa accatgtcct catgacatct atcaataacc
    11161 catgtaaaaa tatacttaag aaattcttct gccagagtgc aatcatcttc cagggttgca
    11221 aacgccatct cgggttccac catccagaat tcggatagat gccgtgtagt gttggagttt
    11281 tcagctctaa atgttgggcc aaaggtatag atatcaccca tcgctaatgc taaaagctca
    11341 ccttcaagtt gaccactgac ggtaagcgct gcttttgctc caaaaaaatc ctggctataa
    11401 tctatgaaac catcctgtac tggtacatgc agcagatcaa gggtggttac ctgaaacatc
    11461 tcaccagctc cctcacaatc acttgttgta ataatggggg tatgaacata taaaaatcct
    11521 ttttgctgaa aaaatgtgtg gatcgcatat gatacttgat tgcgtatgcg caggacagct
    11581 cccatcgtat tggttcttgg acgcagatgg gcaatctccc gtaaaaattc aaaagaatgt
    11641 cgcttcttct gcaggggata gctctcggga tcagcatcac cgtatacgat gagcttatcc
    11701 ggcttaagtt cccacttctg tcctgctgca ggagatgcta ccagtttgcc tgttatataa
    11761 acgctggcac cggtatgcag ctgccgtgca gtttcatagc aactatcgcc aaaaccaacc
    11821 accacctgca tattggttaa acacgagcca tcatttattt caataaagat attttctttg
    11881 gattcccttt tggtacgcac ccagcctgcc accgtgcagg actccaaact tttatcactt
    11941 tgtaaacaat caacaatacg tatgcgcttc atcacctgct ccgctgtcat gttttaattt
    12001 tttgattcca tgctaataac agcactttca ctcattttga acattcaata tagcaaacat
    12061 catcaattca aaaataaaaa ataatagaag tactatttgt tttatattac atcattgccc
    12121 ttccttacgc ttctttaata acagtgcttc ctttaatccc ttttcacgta tcactttaat
    12181 ctttaaacct aactcggtca ttttaaaata tcgttttcca tattttgaaa gcaacttttt
    12241 gtcaatctca aaacccaatc ctggacttac aaatggttgc agcactgcat ttttatctgg
    12301 cagtataggt tctattatga gtttgcggat atccgggcat ccacgttggc cagaatgtat
    12361 accgtaaagg tatcgaaaca tggtataact ctatacaacg tatcttcata ataaccctcc
    12421 tcctttttaa ttttttttga ttctatcatc caataatcat gcgtcaatca taattcataa
    12481 tacctgttgt taccccgacg tttttttaca gcaacaacat ctggcatatg aaaaaaaata
    12541 atttacaggt gatttttatt tttttaaaaa aatatggtta atcattttta atagattatt
    12601 tattattgac aatattttat aaacagacac tttataagta ctatatatgt accgatagtt
    12661 attgacaata gatctattag cagaggaaaa tcatgcgatt attgtatatt actttattat
    12721 tactgtttac tgtcagtacg ttgcatgcgc aggatacggt gtgtgttctg gtatatcata
    12781 catttatggg aaaaaagatt cactatgaca tctcactgga agaattccac aaacagttgt
    12841 tggaactgaa agatcatggt tttacctttg ttacctttca tgaaatcaaa aacggactgg
    12901 taaaaggcaa taaaaatatt ctggtaacca tcgatgatgg gcatagaaca gctcttgacg
    12961 cctattattc agtgttaaaa ccaatgagga ttaaaccgtt attgggaatc aatactttta
    13021 ttattggtaa aaagccgtat gttttgacat gggatgagtt aaaacagctt tctcgtgaag
    13081 gctgctatat agcctcacat ggttacttcc atctttttat aaatgataag ctttataaaa
    13141 acaactatcg ttattttaaa ttagaagtat ttgaatcaaa aaaaatgctg gaggaaaaac
    13201 tatgcacacc cgttgatacc tttatatatc cttttggtgt tgtttctgac gttggtaaaa
    13261 aagctttgat tgaagcggga tataactacg catttactat caaatgggga aatacttcat
    13321 tgccaataac aagcaataat ctggaaatat cccgatacat gtataatcaa aactggaacg
    13381 ccatagccag agccattatt tataaacaca aaaacccatc tacccctcat aaagagagcg
    13441 aaaagattct tgtttctacc atgccccggt tgaaaggtac tcatcaatag ctttggctgc
    13501 ctttcgtcct gcacccattg cctgtataac tgttgcagca ccggttacaa tatcgccgcc
    13561 agcaaaaacg cctatcttgc ttgtcttcat ggtttcggga tcggctatga tatttcccct
    13621 ctttgttata ttcatgccgg gtgttgtttt ggggattaac ggatttgggc cattcccaat
    13681 ggcaataatg gctacatcaa tagcaatggt aaactctgaa cctttcacag ggattgggct
    13741 tcgtctgcca cttgcatcag gttcacccaa ttccatcttc agacactcaa ctgctttcac
    13801 acgtcctttt tcatcaccaa tgaactgtac aggattggtt aacaatgtaa aaataacgcc
    13861 ttcttcctcg gcacgttcca cctcttctac tcgtgccggc atctcagtgc gggaacgccg
    13921 gtaaacaata tacacctcct tgcttcccaa acgcagtgca gtccgtgccg aatccatggc
    13981 aacatttcct ccgccaaaaa ctgcaacctt ctgccctttg acaatgggag tatcatactc
    14041 cgggaattta tacgccttca tcaaatttga gcgtgtcaaa tactcattgg cactgtacac
    14101 cccctgtaaa ttttcaccgg gtatatctaa aaataatggc aacccggctc ctgtagctac
    14161 aaacacagca tcaaaccccg ttgacagtag atcatttacc gtttcagccg tgccaattac
    14221 cctgcttagt ccaatagtta cacctgcagc tttcagtgca tcaatctcat actgcacaat
    14281 atctttggga agtctgaact caggaatccc atacaccaga acgccaccag ccttatgtaa
    14341 tgcctcaaaa atggtaacat cgtaccctat acgtgctaaa tcaccggctg cagtaagccc
    14401 tgctggcccg gagccaatga ttgcaacctt tttattggta tggctatcgc tcttctgagc
    14461 cacaaaatgt tccttctctc gttcataatc agcaacatag cgctccagat tgccaatagc
    14521 aacagcatcg cctttttttg ccagcacaca caatttttca cattgatctt cctgggggca
    14581 taccctcccg catactgccg gcaatgaatt tgtttgtttt atggtaaaaa atgcctcgag
    14641 aggccttccc tctttcaacg cctttataaa tcctggaata ttgacattaa caggacatcc
    14701 atcaacacag gcaggacgct tacactgcaa acaccgtgct gcctctacca tagctgtttt
    14761 ttcatcatac cctaacggga cctcattgaa attgcggata cgattttttg catcctgctg
    14821 aggcattttc tgccgtggaa ttttatctgc catagtactg ctccagtttg catttatgtt
    14881 tttcaaagct ttctttttcc tgctctttat aggtattaag ccttttcata agttcatcaa
    14941 aatcaaccag atgagcatca aactcaggac catcaacgca ggtaaattgc tttttgccat
    15001 caaccgtaac cctgcatccg ccgcacattc cggtgccatc caccattatg gaatttaagc
    15061 tgaccagtgt tttcacccca tactgtttgg tcatatcact gaccgccttc atcattggaa
    15121 ccggcccaac agcaacaaca atatcaactt tccccccatc aagtatcgtt ttcaattcat
    15181 cggttacaaa tcctttaaca ccatgcgacc catcatccgt agttactatc atctcatcac
    15241 tttttgcttt gagttcgttt tctaatatta ataaattttt gttgcgcgct ccaataatac
    15301 ttatgagata gttacctgca tctttcattg ccactgcaat tggatacaca gcagcaattc
    15361 caatgccacc accaacgcaa acacaggtac catagcattg tatatcggta ggatgcccca
    15421 atggtcctgc aacattaaaa agttcctccc ccttcttttt aagcgatagt ttatgggtag
    15481 aggtccccac aatctgatag taaagtgtga tagttcctgt agcactatca gcatcagcga
    15541 tagtaagcgg gatacgttca cccacttcat caacaataac tattacaaat tgccctgctt
    15601 ttctgtgttt tgcaatatag ggtgcttcca caatagcttt tcctacattg tcagatattt
    15661 tttcattggt tactatcgta tacataattc acttccataa taaagataat caaacaattg
    15721 atatgcataa tgaaatattg aacatgtcat gaacttacta gcaatcatct gacctttgac
    15781 agacatagta tatagtatat cacattctat tgattaaaaa ataactatcg ccaatacaat
    15841 actgtcacaa atactccctg tcaaaaagca tataccaaca ctgatgacta tacaccattt
    15901 gttgcaggca aacagtacac tataatagct acacatggtc aagatatttt tatataatca
    15961 ggctgtcgaa ataatgtaca taacaaggat agtatgccat tagtataccg taatcaagcg
    16021 acaaaaacag ggagaacgtg atgagacgat tcagggaacc gcgtcataga ccactcgacg
    16081 atagggaagt ttttggttcg tttcacaaac gagcttatag agatgtttgt acaaacggat
    16141 aagcttgcca ttgagaaggg gctggcgaat tgtaaagtat tgtcaattga ttcggtcaag
    16201 atacgggcga atgcgtcaag caagaaggac acgagcattg atggtttgaa cctgctggaa
    16261 gacaccatta aggagaaact accgagattc atggatgatg tggaccgaga tgagcgcagg
    16321 gatgaagaaa ttaaggcgtt gcatcgtcag gaggcacggg tagccgaggc aaacaaggaa
    16381 ctgcggatgc tggttttgca tctcttgata attacgaaaa acttgctgcg atgaatcagg
    16441 aagcattgat acctgacaga tggtatgatg ttgacaggcg tggtgttcag aagaaaggag
    16501 cgtttgatac atgtca
//
LOCUS       DAOOJH010000050        16459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3395_length_16459_cov_9.362777, whole genome shotgun sequence.
ACCESSION   DAOOJH010000050 DAOOJH010000000
VERSION     DAOOJH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_16459_cov_9.362777"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLJ75_07745"
     CDS             complement(<1..511)
                     /locus_tag="PLJ75_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HPD05144.1"
                     /translation="MLYCQAQSFQAVRQAIALSLATLKECTITIAPQVFTHPHYGRMV
                     HSLINVLQQLSVAISITDTTITLKPHQLQSFTATIALHPYCPIADIFLCMAPALIKNE
                     VQSDILFHGVTHSQFSHSTGFIRYGLAPLLQQFGCYLYSATKKFGFYSATGTAATKVY
                     PALHKNAGAI"
     gene            complement(511..1779)
                     /gene="serS"
                     /locus_tag="PLJ75_07750"
     CDS             complement(511..1779)
                     /gene="serS"
                     /locus_tag="PLJ75_07750"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPD05145.1"
                     /translation="MIDPKIVRDNIDLVKHTLKIRRMEDAVDIAKLEDMDATRRQIIA
                     KIDELRTMRNTLSKEVGTLKQKGQNPTDIMEKVRSIGNDIKQYEEQIASIEETYTTMM
                     LSIPNMLHESVPIGNDEHDNVVVRTWGQKPYFDFEPKPHYDLGTQLNILDFERGVKIA
                     GARFYVYRGLAAQLERAIINFMLDLHTKKHGYTEVFGPFIVNDESMIGTGQYPKFMDE
                     YYRIERDALSLIPTAEVPLTNLYRDEIIDGNNLPLYVTMQSSCFRREAGAAGKDTRGL
                     IRVHQFQKVELVKFVEPETSFDELERLTADAEEVLQLLNLHYRVVLLCSADTSASSSK
                     TYDIEVWMPGLDRYVEISSCSNFVDYQARRARIRYRKKEGEKPMLVHTLNGSGLAAGR
                     TLAAIMENYQTRDGNITIPELLKPYLEKSI"
     gene            2305..2451
                     /locus_tag="PLJ75_07755"
     CDS             2305..2451
                     /locus_tag="PLJ75_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05146.1"
                     /translation="MRKQNRFNEQKAGGMTVYMQEQQQHQRWRQKSRGMVIVIVKQQH
                     ESGQ"
     gene            complement(3153..3225)
                     /locus_tag="PLJ75_07760"
     tRNA            complement(3153..3225)
                     /locus_tag="PLJ75_07760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3190..3192),aa:Phe,seq:gaa)
     gene            3264..4190
                     /locus_tag="PLJ75_07765"
     CDS             3264..4190
                     /locus_tag="PLJ75_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05147.1"
                     /translation="MSIQKFLMIIFLWVIIAFLFPLPMYADEDAGYYYQKAQLQYQHQ
                     MYTYAVENLERVLIITPKHTAALNLLATIYLNHYNNRVRALQYYNQSLAIDDNQPDVH
                     CEVGKLYVYFSEYNHAIEHFTKTIAQQGKAVEAHYHLALVYNTLKQYGTAAVHADECN
                     RLTKNDTDMLLTKAADALQKNEKGVAIAQYSRALQVNPACKEAYIGLALLYRQDNSLA
                     KAIEVLEQCIKVYPHDNTTAMTLAHLCFEYKPAKRRKYYIDRAITLCKGVVERDPFHC
                     EAYSLLYDVYKTLHDVPLMDNYAEEYNRCCEK"
     gene            4408..4710
                     /locus_tag="PLJ75_07770"
     CDS             4408..4710
                     /locus_tag="PLJ75_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD05148.1"
                     /translation="MDSKVEYIIQKIVSIIVEKADPARIILFGSRAKGNAHKGSDIDI
                     ALEGEKALTFREMRKMKELIDDVSGLYSVDIIVLRKVDDTFKDSIKESGVVIYEKK"
     gene            4697..5089
                     /locus_tag="PLJ75_07775"
     CDS             4697..5089
                     /locus_tag="PLJ75_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007474707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HPD05149.1"
                     /translation="MKKSEIYLSVKKLENAVTSLKEAVSIAQTDLEKDGTIQRFEFTV
                     ELLWKTLKTILLYNGVECNNPRACIKEAFRNRIIEDDEILLDMLEDRNVSAHIYDEIT
                     SVEIFERIKNVYSDILENITKSIQSGIK"
     gene            5138..6502
                     /locus_tag="PLJ75_07780"
     CDS             5138..6502
                     /locus_tag="PLJ75_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPD05150.1"
                     /translation="MNSNKTMNLKRVLGLAEVTFIAVGFTIGGGIFVFTGIVLKIVGP
                     ALPLAYLLAVIPVFLSMLPLAMLGSAIPSVGGNYKYPSRMVSPGVAFAGIWIYVLATF
                     FGQIPLYSISCARYIATFFPAMSVQCMAIVLVTVFYLVNLIGIKPASVLQAIMVIILA
                     SALLFFSFAGIVHLDGKNFENFFQKGIGNLAVGIALLTFTYLGSNGIIELGGEIKDAG
                     KTIPRAYFITFPIVAIVYLLVAIAATGALPWHTTSQAEEPLVFISKSILSSAGFMFFI
                     LGGAVLALITTLNALFIVGTKSLLIIINDRLLPAWLGNVHKKFATPHILLTIIWLLSL
                     AGILSNFSIETFASYSALGGIIIFLPILIAALRFPKLYPERYMQAHFKLKGVWLYLCT
                     FAGFILIVFFCIIILVDLQSLLKIGLFLLFALSGVAYYYLRKRFLLRQGFDIVAIATK
                     EDWR"
     gene            6506..7576
                     /locus_tag="PLJ75_07785"
     CDS             6506..7576
                     /locus_tag="PLJ75_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPD05151.1"
                     /translation="MKTLPLLDTWEALQQLPWLSLGKFPTPVQRLHHFSHDSLWIKRD
                     DLSSDVYGGNKVRKLEFTLAEAKALNKSRVVTMGGIGTNHGLATAMFCQKLGLACRLL
                     LFYQPVTAYVKKNLLLLCRYNAQLKYYKTMLKAGVMFYTFERLMHDAYFLYAGGSSPL
                     GTVGFVNAAFELQQQIQQGLMPEPDYIVCALGSAGTMAGLTLGAALAGLRSKVIGVRV
                     TDRSLGPVPIANEKSVLSLMKQTYALMAGFTQLPAVDFMTPAIVHDYCGEGYGYPTAA
                     CMDAIDTMHQLENIPLEPTYTGKTFAAVCGMIQNPEYNNKTILYWHTYNSVDLTEEAA
                     HVDYHDLPKNLHWVFEERSNAC"
     gene            7635..9392
                     /locus_tag="PLJ75_07790"
     CDS             7635..9392
                     /locus_tag="PLJ75_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD05152.1"
                     /translation="MSMESNSNNTPAFNDRYITIFENTGAGTILIDADTTIILVNSRF
                     ASMLGYDKHEIEGRVSWTQFIHEDDVERMKEYHCLRRKDPDSAPKNYEFRIYTKDKQI
                     LYIYITIDMIPGTQLSVASLTDITQLVETREALRKSEEKYRLLVETMSDGLLVQDEHL
                     TIQYVNPRFCDIVGYDQQYIIGKQIKDFIHPHYYCQFDIQMEKRQFGIQEPYEMAWMK
                     DDGTIAHTIVSPKIVSDEQGRFLGSFAVVTDITQRKNWEEALKQSEELFAKVFDATPV
                     GLALIDMQTGMIVKWNKAFEILIHVPLHHKPAISIFDIGIIKKRDIQRLYKQLIKFNT
                     ISDYEISLKHDSKEYIAILSAEVIYINDQKMVIISLTDITEKRMLEKEIILISEKERQ
                     TIGQDLHDDLIPHLLGIDALCKVMIQELSKNILPLKEDVENIRQLLDGAIVKTRQFSR
                     GLTPSYFIEKQGLSALVHELIQTVAMIYGITCTYICDKEISISNSEVLTHVYHIIQEA
                     VYNAVKHSKSETINIVITETHKTIMIVIEDKGYGFDIAHVRGMGLKIMRHRAAVIGAE
                     LKITSMVNAGTTISLTIDK"
     gene            9400..10077
                     /locus_tag="PLJ75_07795"
     CDS             9400..10077
                     /locus_tag="PLJ75_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD05153.1"
                     /translation="MNKKISIVIVEDHPVFRMGLCKIINQESDMMVVGEAEDVHNGLK
                     IISKLKPDAAIIDISLKDSNGIELVKDIARHFPVVKTLVLSMHDEKIYAQRVLQTGAS
                     GYIMKDKTSDVIVEAIRTILDGSIYVSEQIMKEIVAKAVKNEYGQSHVDILTNRELAI
                     FALIGKGKTTSEIAATLNLSSKTIGTYKERIKEKLHIKNAVQLVNEATRWVHETEENV
                     SQLPTNK"
     gene            10173..11831
                     /locus_tag="PLJ75_07800"
     CDS             10173..11831
                     /locus_tag="PLJ75_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HPD05154.1"
                     /translation="MENKTYEPGQAYEYQLTLDKLLKAALVYNPNQEIVYRDIIRYTY
                     KDLMRRIHKLANVLKGMGVKQGDTVAIFDYDSHRYLECFFAVPMMGAVLQTMNFRLSP
                     EQIVYTLNHTGATTILVHGDMLPLISSIRDKLHNVCHCIVLVDDVKPLADDIAITGEY
                     EAMIRVADDRYDFPELDENTKATTFYTTGTTGLPKGVYFSHRQLVLHTFGLMAMLGAY
                     DNIGRFRSGDVYMPLTPMFHVHAWGIPYLATLLGVKQVYPGKYEPAMLLKLIVKEKVT
                     FSHCVPTIIQMLVTAPGIESVDLSHWKVVIGGAMLPEDLCKQALDKGIDIYSAYGMSE
                     TCPLLTAALLKPTLLSIPKDQQVAFRCKTGLPAVLADVQVVDAHDNPVEQGMTGEVVV
                     RSPWLTKGYYKDEDKTKELWKNGWLHTGDVGFIDEYNYLKITDRIKDVIKTGGEWVSS
                     LDLENIMSQHPAVFECAAVGVPDPKWGERPLLVIKLKDDCKNTVDDKQLHAFMLKAAK
                     DGKIPKYGVPDKFEFVDAIPKTSVGKIDKKEIRKKYSQPVAATI"
     gene            12011..12472
                     /locus_tag="PLJ75_07805"
     CDS             12011..12472
                     /locus_tag="PLJ75_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPD05155.1"
                     /translation="MQCEVPAFNPGDSEIKNILLNNKIIAVVGLSDDPAKDSNRVARY
                     LINHGYTVVPVNPTKQSILGLQSYPNIKAIPFPVDIVDIFRKPADVPPIVDDAIAIGA
                     KVVWMQLGIAHNEAAKKANNAGLKVVQGKCIKVEHSRYFGNSGQTVQFNIS"
     gene            complement(12487..13890)
                     /locus_tag="PLJ75_07810"
     CDS             complement(12487..13890)
                     /locus_tag="PLJ75_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPD05156.1"
                     /translation="MNNNERLAIIQQKLSAALGASVVSNDPDVLEKYAIDETSDLKGN
                     PWLVVYAKSAEDVSTVLSVCNEYCIPVIPRGAGTGVTGGAVPVCGGVVLSLETMNSIV
                     EIDVENMIAVCEPGVITGILQKEVAQYGLMYPPDPASLDSCSLGGNVAEGAGGPRAVK
                     YGTTKDYITGLEFVLSDGSIIQHGGKFIKNATGYNLTGILIGSEGTLAVITKIYCKLI
                     PAPAYTLDMLIPFASLEEAIRMVYTFLINRIKPAVLEFMEEDALIVASQYLGQAMPHP
                     EAKAHLLIQLDGDSSEDIERMLQTILSLGMVHPDTILTAQSPQQKERLWKARRCIREA
                     IDAKSPVFLAEDCSVPRSSIAPFLTGVKEALTPYGLQSIIFGHAGDGNVHIDVLKNDM
                     PYEDFKKLVPALKEIIYGQAYRYGGTITGEHGTGFIRKDALKKFATSQELSLYKRLKK
                     AFDPNGILNPHKIIDID"
     assembly_gap    13980..14079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14239..14436)
                     /locus_tag="PLJ75_07815"
     CDS             complement(14239..14436)
                     /locus_tag="PLJ75_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05157.1"
                     /translation="MKLTKEIEQGQADLSIEIEKIQTDLTREIHQSHTSTLRWMIGLF
                     LAAVLTQAGLIIALLQLAISK"
     gene            complement(14988..16178)
                     /locus_tag="PLJ75_07820"
     CDS             complement(14988..16178)
                     /locus_tag="PLJ75_07820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05158.1"
                     /translation="MHHKQKLYTNPNLIIAFSITLIVVMGVTNITPALPAMATFFNIP
                     YSSVALLITVFTMPGIIVTPILGIVSDRIGRRILIIPSLLIFGITGTIMFFVKDFSWL
                     LILRFIQGVGVSALGAINTTIIGDMFTGNDRTAAFGYNASVLNIGTAAYPVIGGLLCI
                     AGWNYPFLLSAFAFVVAWFVIFHLHNPEPVNEVALKDYFKTIAAIIKKPYIIGLLSTN
                     ILTFTLLYGPIITYFPYIISYRFNGDSATIGLIMSLMSIVTAITATQLQWLSTRFSEK
                     NLIFYSFLGFAISFIIAGLAQNYFWLTVAIIIGGASNAINNPSLLSLLTGAAPTHYRG
                     AIMSLNGMGLRVGQTVGPIIMAMLLAIVTIDIAFYIIGGATVIITIALFSTLLSNREY
                     TVKT"
BASE COUNT         5068 a         2917 c         3584 g         4790 t
ORIGIN      
        1 ctatcgcccc ggcattcttg tgtaatgcag ggtatacctt tgtggctgct gtgccagtag
       61 cagaataaaa accaaatttt tttgtggcgc tatacaggta acatccaaac tgctgcagca
      121 gcggtgctaa gccatagcgt ataaaaccgg tagagtggga aaactgggag tgggttacac
      181 cgtgaaaaag aatatctgat tgcacctcat tttttatcag ggcaggagcc atgcaaagaa
      241 aaatatcggc aatgggacaa tacggatgca gggcgatagt tgctgtaaaa ctttgcagct
      301 gatgcggctt gagggttatg gtggtatccg taattgagat ggcaaccgat agctgttgca
      361 atacattgat aagcgagtgc accatcctgc catagtgagg atgggtaaaa acctgcgggg
      421 caatggtgat ggtacattct ttcagtgttg ctaatgacag agctattgcc tgccgcacag
      481 cctgaaacga ttgtgcctga caatacagca ttatatgctt ttttccagat agggtttcag
      541 cagttcgggt attgttatat tgccatcgcg tgtttgatag ttctccataa tggctgccag
      601 tgtgcgccct gccgcaagcc ctgaaccatt gagtgtatgt accagcatgg gcttttcgcc
      661 ttcttttttg cggtagcgga tgcgcgcacg ccgtgcctga taatcaacaa agtttgagca
      721 tgatgatatt tcaacatatc gatccagtcc gggcatccat acctctatat cataagtttt
      781 agaagaagac gctgatgtgt cggcactgca caggagcact acccggtaat gcagattgag
      841 cagttgcaaa acctcttctg catcggctgt caacctttcc agttcatcaa atgatgtttc
      901 gggctctaca aattttacca gctcaacctt ttgaaattga tgtacccgta taagtcctcg
      961 tgtatcttta cctgctgcgc ctgcctcgcg tctaaagcat gatgattgca tggtaacata
     1021 caggggcaaa ttattcccat ctataatttc atcacggtac agattggtta atggtacttc
     1081 ggcagttggt atcagtgaca gcgcatcacg ttcaatacgg taatactcat ccatgaattt
     1141 tggatattgc ccggttccta tcatgctttc atcattaaca atgaatggcc caaatacctc
     1201 ggtgtagccg tgcttttttg tgtgtaaatc aagcataaaa tttataatag cgcgttcaag
     1261 ctgtgcggca agtccgcgat atacgtaaaa gcgtgctccg gcaattttta ctccacgttc
     1321 aaaatccagt atattgagct gtgtccccag atcatagtga ggttttggct caaaatcaaa
     1381 atatggtttt tgcccccagg tgcgcacaac aacattgtca tgttcatcgt taccaatggg
     1441 tacggattca tgtaacatat tagggatgga taacatcatg gtggtatagg tttcttctat
     1501 agaggctatc tgctcttcat actgctttat atcgttgcct atactgcgaa ctttttccat
     1561 gatatcggtt gggttttgtc ctttttgttt taatgtgcct acctcttttg agagcgtatt
     1621 gcgcattgtt cgcagctcat ctatttttgc tataatttga cggcgtgtag cgtccatatc
     1681 ctccagtttt gcaatatcga cagcatcttc catcctgcgg attttcaagg tgtgcttcac
     1741 cagatcaata ttgtcgcgta ctatctttgg atctatcatg gtaaaacctc atggtgcatt
     1801 aaaatagtat gtttacaatg aaattgcaca tgtacaatgt aatactgtag cacacatgca
     1861 atgctgataa aaccattttt ttacactgat aatcatataa aaaagatggt catcaaatat
     1921 gtctttcatg aaataggaat gttaagtaat tttgtcaaga aagtatggtg tatgtaagct
     1981 ataaaaaaca agaaaaaatt gtatatctgt aacgctttac agcaaaaaga aaatcattca
     2041 ggttatggat gtacggtgtt aggttcttat tgttgaatgg ttgtgtagat tcataaagtt
     2101 ttaatgcgaa ataatggatt tattggaatt tgtgcacctc taattaccgt tattgaatat
     2161 gactgaggtg tgtataaaaa ggcagctata taacgatatg agcttttgtg gtataataag
     2221 ggagagcatc atcttttcaa gatttctcca taaaaatttt attattttac aatttacttt
     2281 ttcgattgca tctggcacaa tgaagtgcga aaacaaaacc gttttaatga gcagaaagct
     2341 ggcgggatga ctgtatatat gcaggagcaa caacagcatc aacgatggcg acagaaaagc
     2401 cggggtatgg tgatagttat agtgaaacaa caacacgaaa gcgggcaatg aatagtatta
     2461 tggagaggag catggatatg gcagtagaga acaccgtcat ggcagggtat gccgtaacaa
     2521 aaggcgacgg tgtagcgatg gtgctggaca taacgtttca atgggaaatg agaatttttg
     2581 tggaaaaaaa atagcgagtg atctccttaa atttatttta aagcttatac aatagaggcg
     2641 aggtaatagc agagtacagt atatggaaca ggacattcat tgagtgcatc accggcctga
     2701 atggagcaat taaataatgt tccgttcttt cttttgtcac aaaagcgcaa caaaagaaag
     2761 taccaatgaa aagttgtcag ggcgggcgag gaagcccggt gtgcacccgc cgtggaaggg
     2821 atatatgtga tgcaatgaag gcacaccgcc gtttaaggtg gctccacagg gttagtaagg
     2881 gaatattgag cgcataaatg ctcacttacg aaagtgaaaa cgcagattgc cttatgcagg
     2941 gcaccataga gaatgccata ctggcaggag cagacatgat gaagggaaaa tccatgcaga
     3001 atgcggtggt atatcaaaac tgggagaaga cgcagtacag ggcagcagac tgtttaagcg
     3061 actgggcagc acaaaaaggg cgccacgatg cagaagactg gaagaccaat cagcagaaat
     3121 tgtgtgacag gcacaataga gaaaataaaa attggtcagg gacggaatcg aaccgccgac
     3181 acggggattt tcagtcccct gctctaccga ctgagctacc tgaccagcca ttatggatgg
     3241 atacataaag aaggtgttat aaaatgtcaa tacaaaaatt tttgatgata atctttttgt
     3301 gggtaatcat agcctttcta ttcccattgc ctatgtatgc cgatgaagat gctggttatt
     3361 attatcaaaa agcgcagttg caataccagc accagatgta cacatatgct gttgaaaatc
     3421 tggaaagggt actcatcatt accccaaagc atactgcagc attaaatctt ttagcaacta
     3481 tctacctgaa tcattacaac aatagggtca gagcattgca gtattataac caatcgcttg
     3541 caattgatga taatcagcct gatgtacact gtgaggtggg aaagctgtat gtttatttta
     3601 gcgagtacaa tcatgccatt gagcatttca caaaaactat cgcccagcaa ggaaaggccg
     3661 ttgaagctca ttatcatctg gcattggtgt ataatacgct gaagcaatat ggtaccgcag
     3721 ccgtacatgc cgatgaatgt aacaggttaa caaaaaatga caccgatatg cttttgacta
     3781 aggctgccga cgctttgcaa aaaaatgaaa aaggtgtggc gatagctcag tatagcaggg
     3841 cattgcaggt aaatcctgcc tgtaaagaag cgtatattgg gctggcattg ctgtatcgtc
     3901 aggataacag tttagcaaag gcaatagagg tgcttgagca atgcatcaag gtatatccgc
     3961 acgacaatac aaccgcgatg acattggcgc acctatgttt tgagtataaa ccggcaaaac
     4021 gccgtaagta ttacatcgat agagctatca ccttgtgcaa gggtgtagtg gagcgtgacc
     4081 ccttccattg tgaagcatat tcattattat acgatgtgta caaaacgctt catgatgtgc
     4141 cgctgatgga taactatgca gaggaataca acaggtgttg tgaaaaataa atgcgttcaa
     4201 tcaaatacta ttgttttatt agcgtaaacc aacactttct attctatatg tttttttact
     4261 gcccgtgaaa gaacaagcta ttattaaccc tctgtattta tcttgacagt gttatttcaa
     4321 gtcagtataa ttttggatgt caggcgtcat aaagttttaa tgggaaatga tactatgaac
     4381 gattacatgt gatggagata ggatataatg gacagtaaag tagaatatat aatacaaaaa
     4441 attgtatcaa ttattgttga aaaagctgat cccgccagga ttatcttgtt tggctcgcga
     4501 gcaaaaggca atgcacataa aggttctgat attgatatag cgcttgaagg cgagaaagca
     4561 cttacattca gggaaatgcg gaagatgaaa gaactcattg atgatgttag tggattatat
     4621 tctgttgata ttatagtgtt acgtaaggta gatgatacct ttaaggatag tataaaagag
     4681 tcaggggttg taatctatga aaaaaagtga gatatatcta tctgttaaaa aattggaaaa
     4741 tgctgttaca tcactaaaag aagcggtaag tattgctcaa accgatttag aaaaagatgg
     4801 tactatacag cgttttgagt ttacggttga gcttttatgg aaaacattaa aaacgatatt
     4861 attatataat ggtgttgaat gtaataatcc cagagcatgt ataaaagagg ctttcaggaa
     4921 ccgaataata gaagatgatg aaatactcct tgacatgctg gaggatagga atgtgagcgc
     4981 acatatttat gatgaaataa catccgtaga aatctttgaa aggattaaaa atgtttatag
     5041 tgacatcctt gaaaatataa caaagtccat acaaagtggc atcaaatgat tgttcagtga
     5101 tgagcaaaca tagtatacaa tagatggtga aaaagcaatg aacagcaata aaaccatgaa
     5161 cttaaaacgg gtactgggac ttgcggaggt tacctttatt gcagtgggat ttaccattgg
     5221 tggtggtatt tttgttttta cgggcattgt actaaaaatt gtgggacctg cattaccgct
     5281 tgcctatttg ctggcggtga ttcccgtctt tttaagcatg cttccacttg caatgctggg
     5341 gtcggcaatt ccgtcagttg gtggcaatta taaatatcct agccgtatgg tatcaccggg
     5401 tgttgccttt gctggcatat ggatttatgt acttgcaaca ttttttggac agattccact
     5461 gtacagcata tcctgcgcgc ggtacatagc tacttttttc cctgctatgt ctgtgcaatg
     5521 tatggcgata gttcttgtaa cagtatttta cctggtaaat cttatcggga ttaaacctgc
     5581 ttccgttttg caggcaatca tggttatcat cctggcaagc gccttgctgt ttttttcatt
     5641 tgctggaatt gttcatcttg atggtaaaaa ttttgaaaat ttttttcaga agggtattgg
     5701 gaatcttgca gtggggattg cactgcttac ctttacctat ttaggatcaa atgggattat
     5761 tgaattgggt ggtgaaataa aagatgcggg gaaaactata cccagggcat attttattac
     5821 ctttcccatt gttgccatag tgtaccttct tgtggctatt gctgcaacag gtgctcttcc
     5881 atggcataca acatcacagg ctgaagagcc tttagtgttc ataagcaaat caatactgtc
     5941 cagtgccggg tttatgtttt ttatcctggg cggtgcagtg cttgccctta tcacaacgct
     6001 caatgccctg tttattgtag gcactaagtc attgcttatc attatcaatg ataggctgct
     6061 tccagcatgg ttgggaaatg ttcataaaaa atttgccacg cctcatatac tgctaaccat
     6121 tatctggtta ctatcgcttg caggtatcct ttcaaatttt tccattgaaa cgtttgcatc
     6181 gtactcagca ttgggtggca ttatcatctt tcttcctatt cttattgcag cgctgcgatt
     6241 tccaaaactg tatcctgagc gttacatgca ggcacatttt aaattaaaag gggtgtggtt
     6301 gtacctctgt acttttgcgg gttttatcct catagtgttt ttttgcatca tcattctggt
     6361 agatctgcaa tcgctgctta agattggctt atttctactg tttgctttga gtggtgtggc
     6421 gtactattat ttacgcaaac gatttctgct aaggcagggt tttgacatag tagctatcgc
     6481 cacaaaggaa gactggaggt agcctgtgaa aactttaccg ctgctggata catgggaagc
     6541 gttgcaacaa ttaccatggc tgtcacttgg caaattcccg acgcctgtac aacgactgca
     6601 tcatttttct catgatagtc tatggattaa gcgggatgat ctatcttcag atgtatatgg
     6661 cggcaataag gtaaggaaat tagaatttac attagcggag gcaaaggctc tgaataaatc
     6721 aagagttgtt accatggggg gcattggcac caaccatgga ctggcaaccg ctatgttttg
     6781 ccagaagctg gggcttgcat gcaggcttct tttgttttac cagccggtaa ccgcgtatgt
     6841 aaaaaaaaat ttactgttat tgtgccgcta taatgcacag ttaaaatatt acaaaaccat
     6901 gctgaaagcc ggtgttatgt tttatacatt tgaacggttg atgcacgatg cctatttttt
     6961 atatgccggc ggttcctcgc cactgggaac ggtaggtttt gtaaatgctg cgtttgaact
     7021 acagcagcag atacagcagg ggcttatgcc ggagccagat tatattgtgt gtgcattggg
     7081 ctcggcagga actatggcag ggcttaccct gggtgcagcg ttagcgggat tacgcagtaa
     7141 ggttattggg gtcagggtaa ccgatagatc ccttggacct gtgcctattg ccaatgaaaa
     7201 gagtgtactg tcactgatga agcaaaccta tgcactcatg gcagggttta ctcaattgcc
     7261 agcagttgat tttatgacac cagctatcgt acacgattat tgtggtgaag gatatggtta
     7321 cccaaccgct gcatgtatgg atgccattga cacaatgcac cagttggaaa atataccgtt
     7381 agaaccaacc tataccggta aaacatttgc agcggtatgt ggtatgatac aaaacccgga
     7441 atataacaac aaaacaatat tgtactggca tacctataat tccgttgatt taacagaaga
     7501 ggctgcgcat gttgattatc acgatctgcc gaaaaatttg cactgggtgt ttgaagagag
     7561 atcaaatgca tgttgaaaaa gtcatgatac tctatacaat ccttttgtaa aaaaatttta
     7621 tggggcgata gttcatgtca atggagtcaa acagtaacaa taccccggca ttcaatgaca
     7681 gatatatcac aatatttgaa aatacaggtg caggaaccat cctcatagat gcagatacaa
     7741 caattattct ggttaatagc cgttttgctt ccatgctggg atatgataaa cacgagattg
     7801 aagggagggt aagctggacg caatttatac atgaagacga tgtagaaaga atgaaagaat
     7861 atcactgttt gcgaagaaaa gatccggata gtgcacctaa aaattatgaa ttcagaatat
     7921 atacaaaaga taaacagata ctgtacattt atattaccat tgatatgatt ccaggtactc
     7981 aattgagtgt tgcttcgtta accgatatta cccagttagt tgaaacaaga gaagcgctgc
     8041 gaaaaagtga agaaaaatac cgtttgcttg tggaaacaat gagtgatggg ttgctggtac
     8101 aggatgaaca tttaacaatt caatacgtta atccacgatt ttgtgatatt gtaggatatg
     8161 atcaacaata tattattggt aaacaaataa aagattttat ccatcctcat tactattgcc
     8221 agtttgatat ccagatggaa aaacgtcagt ttggaattca ggaaccctat gaaatggcat
     8281 ggatgaaaga tgatgggaca atagcgcata ccattgtatc cccaaaaata gtatccgatg
     8341 agcagggacg atttttgggt agctttgcgg tagttactga tataacccag cgaaaaaatt
     8401 gggaagaggc tttaaaacag tcagaagagc tttttgcaaa agtatttgat gcaactcctg
     8461 ttggattggc gcttattgat atgcaaacag ggatgattgt aaaatggaac aaggcttttg
     8521 agatacttat ccatgtgcca ttgcaccaca aaccggctat tagcatcttt gatattggaa
     8581 taattaaaaa aagagatata caacgattat ataaacagct cattaagttt aatacaatca
     8641 gtgattatga aatttcattg aaacatgatt caaaagaata catcgcaatt ttatcggcag
     8701 aggtgatata tattaatgac cagaagatgg ttattatatc acttactgat attacagaga
     8761 aacgaatgct tgaaaaagag ataattttga ttagcgaaaa ggagcgacag acgattggtc
     8821 aggacctcca tgatgatctt attcctcatt tgctggggat agacgccctg tgcaaggtga
     8881 tgatacagga attatcaaag aatatactgc cgcttaaaga agatgtggaa aacatccggc
     8941 agcttttaga tggtgccatt gtcaaaacac gccaattttc aagagggtta acaccatcat
     9001 atttcataga aaaacagggt ttatctgccc tggtgcatga attaatacaa actgttgcaa
     9061 tgatttatgg gattacctgt acctatatat gtgataaaga aattagtatt tcaaattcag
     9121 aagtattgac gcatgtgtac catattattc aggaagcagt ctacaatgca gtaaaacaca
     9181 gcaaatctga aacaataaac attgtgatta ctgaaacaca taaaactatt atgatcgtta
     9241 tagaagataa agggtatggt tttgacatcg ctcatgttcg tggtatggga ttaaagatca
     9301 tgaggcacag ggcggcagtt attggtgcag aattaaaaat aacttcaatg gtaaatgccg
     9361 gaacaacaat atcgctaact atcgataaat aagggtacta tgaacaaaaa aatatctatc
     9421 gtaattgttg aagatcatcc cgttttcagg atgggcttgt gcaaaataat taatcaggaa
     9481 agcgacatga tggttgtggg tgaagccgag gatgtgcata acggattaaa aattatatca
     9541 aaattaaaac ctgatgctgc tattattgat atatcgttaa aagatagcaa tgggattgaa
     9601 ctggtaaagg atatagcacg tcatttccct gttgttaaaa cattggtttt atcaatgcat
     9661 gatgaaaaga tttatgcaca aagagtattg cagactggtg catcaggata tattatgaaa
     9721 gataaaacat cggatgtaat agtggaagcg attagaacca ttctggatgg gtctatctac
     9781 gttagtgagc agattatgaa agagattgta gctaaagccg taaagaatga gtatggacag
     9841 tcacatgttg atatactgac aaacagagag ctggcaatat ttgcattaat aggaaaagga
     9901 aaaacaacca gcgagattgc agcaacactt aatttaagca gtaaaactat aggaacatat
     9961 aaagaacgca taaaagaaaa attacatata aaaaatgcag tacagcttgt taatgaagct
    10021 acccgatggg tgcatgaaac tgaagaaaat gtaagtcaat tacctacaaa taaataagta
    10081 tcaatggtac atcaataaaa taattccaag tattgtaaga caatatcatc gttgttacta
    10141 tctctataaa taaaacagca ggaggtttat tgatggaaaa caaaacgtat gaaccagggc
    10201 aggcgtatga gtatcagcta acgctggaca aattattaaa ggcagcactc gtgtataatc
    10261 ccaaccagga aattgtatat cgagatatca ttcggtatac ctataaggac ttaatgcgtc
    10321 gtattcataa acttgcaaat gtattgaaag gcatgggagt gaaacagggt gatactgttg
    10381 ctatctttga ttatgatagt caccggtatc tggaatgttt ttttgctgtg cctatgatgg
    10441 gggcggtgct gcaaacgatg aattttagat tatcacctga acagattgtt tacacattaa
    10501 accataccgg agccacaact attctggtac atggtgatat gctcccattg attagcagta
    10561 tcagggataa gctgcataat gtttgccatt gtatagtgct tgttgatgat gtgaaaccat
    10621 tggcggatga tattgcaata accggtgaat atgaagcaat gatacgggtt gctgatgaca
    10681 ggtatgattt tccggaattg gatgaaaata caaaagcaac aacattttat accacaggaa
    10741 caacaggttt gccaaagggg gtttacttta gccatcggca actggtcctc catacatttg
    10801 ggcttatggc aatgcttggc gcctatgaca atatcggcag attccgatca ggtgatgttt
    10861 atatgccatt aacacctatg ttccatgtcc atgcatgggg aatcccctac cttgcaacat
    10921 tgctgggtgt aaaacaggtt tatccaggga aatatgagcc tgcaatgtta ttaaagctaa
    10981 tagtaaaaga aaaggttact ttttcccatt gcgttccaac cattatccag atgcttgtta
    11041 ctgctccagg gattgagagt gttgatctat ctcactggaa ggttgttatt ggtggagcaa
    11101 tgcttccaga ggatctatgt aaacaggcat tggataaagg tattgatatc tattcggctt
    11161 atgggatgtc tgaaacatgt ccattgctaa ctgctgcgtt attaaaacca acactactat
    11221 caataccaaa ggatcagcag gttgcgttta gatgcaaaac agggttacct gcagttttag
    11281 cggatgtaca ggttgttgat gctcatgata atccggtgga gcaaggtatg accggggagg
    11341 ttgttgtgcg ttccccatgg cttacaaagg gatactacaa agatgaagac aaaactaaag
    11401 agttatggaa aaatggatgg ttgcatacag gggatgtggg atttattgat gaatacaact
    11461 atttgaaaat aacggacaga attaaagatg ttatcaaaac aggcggtgaa tgggtatcgt
    11521 cgcttgatct ggaaaatatt atgagtcaac atccagccgt ttttgaatgt gcagcggttg
    11581 gcgtcccgga tcccaagtgg ggtgaaaggc cattattggt tataaaacta aaagatgatt
    11641 gtaagaatac agtcgatgat aaacagctcc atgcctttat gctaaaggct gctaaagatg
    11701 gtaaaattcc aaaatatgga gttccggata aatttgagtt tgttgatgct atccctaaaa
    11761 caagtgtagg aaagattgat aaaaaagaga tcaggaaaaa atatagccaa ccagtggcag
    11821 caaccatata atgccagaag ggtcagagca tatgccttca ctatgtgatg ctctgacccc
    11881 ctgttttcag aaaagcatag gtatattcac atatttaaat tgtaaaaaaa tgaaaatgtg
    11941 tttatattta ttatagatta tagtactgca tattgaataa aagccgggga taggaggtgg
    12001 tatggcagat atgcaatgcg aagtacctgc attcaatcca ggtgatagtg aaattaaaaa
    12061 cattttgttg aacaataaaa ttattgcagt ggtgggattg tctgatgatc cagcaaaaga
    12121 tagcaatcgt gttgcacgct atttaattaa tcacggctac acagtggtgc cggttaatcc
    12181 aacgaaacaa agcatattgg gtttgcagtc gtaccccaat ataaaagcaa tcccgttccc
    12241 tgttgatatt gtggatattt ttagaaaacc tgcggatgtc ccgcccattg ttgatgatgc
    12301 cattgcaatt ggtgcaaagg ttgtatggat gcagttaggg attgctcata atgaagcagc
    12361 aaaaaaagct aacaatgcgg ggcttaaagt agttcaaggc aagtgcatta aggtagagca
    12421 ctcccgttat tttggcaaca gtggacaaac agtacaattt aatattagtt aataatgtta
    12481 tcggcatcaa tctatatcaa taatcttgtg cggatttaaa attccgttgg gatcaaaggc
    12541 tttttttaaa cgcttataaa gcgatagttc ctgggaagta gcaaacttct ttaatgcatc
    12601 tttccgtata aatcctgttc catgctcgcc agtgatagta ccaccgtatc gataagcctg
    12661 cccgtatata atctctttaa gcgctggtac cagcttttta aaatcctcat agggcatatc
    12721 gttttttagc acatcaatat gaacgttccc atcaccggca tgcccaaata tgatcgattg
    12781 taagccatac ggggtcagag cctctttgac ccccgttaaa aaaggagcaa tagaagagcg
    12841 ggggactgag caatcctcag ctaaaaaaac ggggcttttt gcatctattg cctcacggat
    12901 acaacgccgt gccttccata gccgttcttt ttgttggggt gattgtgctg tcaatattgt
    12961 atcgggatgt accattccaa gggaaagaat cgtttgcagc atgcgctcaa tatcttcgga
    13021 actatcgcca tcaagctgaa tgagcagatg agcttttgct tctggatgag gcattgcctg
    13081 acctaaatac tgtgaggcta ctataagggc atcctcttcc ataaattcta aaacagcagg
    13141 cttaatgcga ttaattaaaa acgtgtacac catcctgatt gcttcttcaa gacttgcaaa
    13201 tgggataagc atatcaagtg tataagccgg agcagggatg agcttgcagt atatctttgt
    13261 aataacggca agtgttcctt cagaaccaat gagaatgccg gtaagattgt accctgtagc
    13321 attttttata aatttaccac catgctgaat gatacttcca tcactaagta caaactcaag
    13381 ccctgtgata taatcttttg ttgtgccata ttttactgcg cgaggtcctc ctgcaccttc
    13441 agctacattg ccaccaagtg agcatgaatc aaggcttgcc ggatcgggcg ggtacataag
    13501 tccatactgt gctacctctt tttgcaatat acccgtaatg acgccaggtt cgcacactgc
    13561 tatcatattt tcgacatcta tctcaacgat gctattcatt gtttctaatg ataaaacaac
    13621 accaccgcaa acaggtacag caccgccggt aacaccggta cccgccccgc gtggaatcac
    13681 cggtatacaa tattcgttac agactgaaag aacagtgctc acatcctcag cactttttgc
    13741 atacactacc agccatggat tgcctttaag atcggatgtt tcgtcaatgg catatttttc
    13801 aagcacatcg ggatcattgc tgacaacaga tgctccaagt gcagcgctca acttttgttg
    13861 tataatggct aacctttcat tgttgttcat gatgacatac atagggtatg cagtgtattg
    13921 atgcaagctt ttttaggagt gggaatacta ttcaatctat gtcattttgt ctatgagcan
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna agctttttta ggagtgggaa
    14101 tactattcaa tctatgtcat tttgtctatg agcagggtat tgttgtgcaa gggggtgtag
    14161 aatgattttc tattttattt tttattattg taaatattca ggctttttgt gttaaactgt
    14221 atgatatgta cattaacctt atttacttat agcaagctgc aacaacgcta tgataagccc
    14281 tgcctgtgtt aacacggcag cgagaaataa acctatcatc cagcgtaatg tgcttgtatg
    14341 gctttgatgt atctctctgg ttaaatccgt ttgtattttc tcaatctcta tacttaaatc
    14401 tgcctgtcct tgctctatct ctttggtcag cttcaattct gagatcgtta catcctgccg
    14461 ggtggctaac tgattattta atattgtctg ctcaactaac tcaataaact cagctaatag
    14521 ttttgcctta atctcgtctt ccttaaatgc ctcatatatt cttactgctt atcccattgg
    14581 tacgttgcct cctttatgta tatatactac tgcctgctgg taacatgtca aattatttta
    14641 atgcatgtat aataccatcc atacttcact accatctctt tttttaaaaa attgacatga
    14701 atcaacattg taacctcaat aacagggggc gatggtgaaa aaaagtatta atttttttcc
    14761 ttctggaggg ttgtataaca ctaataacaa cgaggttggt gaagaatgag attgccaata
    14821 tgggaataag tatttgataa agcacagttg aacggcaata gtcataataa acaagcgata
    14881 gcaacggcat ggcagattga gcagataaca gcctgaagtt tctcaggaat gagtagttcc
    14941 gctatcctgg gtagtacgta caatcaacga gaggtatcga tagttaatca tgttttcacg
    15001 gtgtattctc tattcgatag cagggtggaa aacagtgcta tggtaatgat taccgttgcc
    15061 ccgcctataa tgtagaatgc tatatcaata gtaactatcg ccaataacat tgccataatg
    15121 attggaccaa ctgtttgacc aacacgaagc cccatcccat ttaatgacat gatggctccg
    15181 cgatagtgtg tgggtgcagc gcctgttaac agtgaaagca aacttgggtt gttgatagcg
    15241 ttggaagcgc cacctataat gattgccacc gtaagccaga aatagttttg agccagccct
    15301 gcaatgatga atgaaatggc aaaaccaaga aagctgtaaa agataagatt tttttctgaa
    15361 aagcgcgttg aaagccattg tagctgtgta gctgttattg ctgttacaat ggacatcagt
    15421 gacatgataa gccctattgt tgcagaatcg ccgttgaacc ggtatgagat gatgtaggga
    15481 aaataggtaa taatgggtcc atataaaagg gtaaaggtaa ggatattggt agacagtagc
    15541 cctataatat agggtttttt gataatggca gctatggttt tgaaatagtc tttcagtgca
    15601 acttcattga caggctctgg gttgtgcagg tgaaatataa caaaccatgc aacaacaaag
    15661 gcaaaggctg aaagcaaaaa gggatagttc cagccagcaa tacataacag tccgccaatg
    15721 acaggatagg ctgcggtgcc aatatttaaa acacttgcgt tataaccaaa tgctgcagtg
    15781 cggtcgttac cggtaaacat gtcgccaatg atggttgtgt tgatagcgcc caatgccgaa
    15841 actcccacgc cctgtataaa gcgcagtata aggagccatg aaaaatcctt aacaaaaaac
    15901 atgatggtac cggttatgcc aaatataagc agtgagggga ttattaaaat tcttcttcca
    15961 atcctgtcgg atactatgcc aaggataggc gttacaataa ttccgggcat ggtaaaaaca
    16021 gtgattaaca gggcaaccga tgaataaggg atattaaaaa aagttgccat agcaggtaat
    16081 gcaggcgtaa tgtttgtaac acccatgaca acaataagcg taatgctaaa tgcaataatc
    16141 aggttggggt tggtatagag cttttgttta tggtgcatag gctaccgctt atgtgtttat
    16201 ggttatcaat ggctatgtat gtaaatatac cagtgtagta ccaatcaagc acaaaacaca
    16261 atgttttctt taattttttg ggcgatagtt acccttatag taagcaaggt gttgctataa
    16321 atattgatta aagccattca ttttttttta tttctttttt attaatgttg atatcgcgat
    16381 atgtgttgaa aattaagaca acccttaaaa aagtattgac aatttgtatt tacgtagtct
    16441 tatgattaat caatagttt
//
LOCUS       DAOOJH010000051        16170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3467_length_16170_cov_9.951101, whole genome shotgun sequence.
ACCESSION   DAOOJH010000051 DAOOJH010000000
VERSION     DAOOJH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16170
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_16170_cov_9.951101"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1188
                     /locus_tag="PLJ75_07825"
     CDS             67..1188
                     /locus_tag="PLJ75_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HPD05159.1"
                     /translation="MKNILLYSETLAQVEYSPTHPFKPMRAKQMLDLLNRYGLLENTE
                     IIEHPVLDEELLYLFHTPDYIHALKTCDHGEFDINLLTYGLGTHDNPIFKGVYNFSLQ
                     ASGGTFLGATKLVDTDATFVFNPIGGFHHAMNDHAEGFCYINDIAIALKHLIHNGLRC
                     AYIDIDVHHGDGVQSAFYDDNRVLTISIHESGQFLFPGTGFENEIGEGAGYGYNVNIP
                     LLKDTDDEIYIYAFEEIVPPLLQSFNPDIICIQVGGDSHKDDPLAHLNLTSNGYQKVL
                     TLIKQQSKKILGTGGGGYNLYKTAALWTLAWSIFTGIKPMDNFMGVIGGMMYGPEADA
                     GSLFDNPYSVGGLTKDKCFDYARDVVAYIKQSVFPIHNI"
     gene            complement(1195..1845)
                     /locus_tag="PLJ75_07830"
     CDS             complement(1195..1845)
                     /locus_tag="PLJ75_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD05160.1"
                     /translation="MIKICAFSDIHRAYENIYRLQPEIESSDIVVIAGDIGPVMWYTN
                     WHAYKRTIAWINKPVLAVHGNWDGSLIQEFLSPYSIHAGFAIVDDVGFFGVGGSIKTP
                     LTTPIEYTEEQIHDYLYKGFEHINHLPVKVLVSHTPPKNMCDRTLFKTHAGSNSVAQF
                     CCEYNINLCICGHIHEACGIGKMGHTTVVNTGSLKRGYYASILVALNGVEATIKRL"
     gene            complement(1982..2140)
                     /locus_tag="PLJ75_07835"
     CDS             complement(1982..2140)
                     /locus_tag="PLJ75_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05161.1"
                     /translation="MKNAQLYIYKEYEEPFECAILPQVDECDRLVSAIKYFGVLIPAE
                     IFDEDRGI"
     gene            complement(2544..3074)
                     /locus_tag="PLJ75_07840"
     CDS             complement(2544..3074)
                     /locus_tag="PLJ75_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05162.1"
                     /translation="MIEHRVEHSYVVKQNFVSRFITPIVLLLVIYIVLHNLYFESWKI
                     DNRDVQYWISFIAGIVLFVFIGFNALIVYPVLYFKGATIHERIVVSLIVQIVWIIKEL
                     VRVSEFFTFGETLYYTFNSAFLLALSGSFAFMGISEMFCRYRLKKKGIELQKIITPLP
                     VMSVVTGIIALYVFLI"
     gene            complement(3067..3414)
                     /locus_tag="PLJ75_07845"
     CDS             complement(3067..3414)
                     /locus_tag="PLJ75_07845"
                     /inference="COORDINATES: protein motif:HMM:NF027666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4976 domain-containing protein"
                     /protein_id="HPD05163.1"
                     /translation="MDLAPTLLDLAGISIPPHIEGKSLKPILLSDKAYGRKAWLYEYF
                     KEFPYNVPEIRAVRTDSHIYIEYGGRRKPELYNVIDDPDERDNLLNTQKGLKLKPELK
                     KLLEDLKMGKQYD"
     gene            complement(3388..4281)
                     /locus_tag="PLJ75_07850"
                     /pseudo
     CDS             complement(3388..4281)
                     /locus_tag="PLJ75_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(4297..4470)
                     /locus_tag="PLJ75_07855"
     CDS             complement(4297..4470)
                     /locus_tag="PLJ75_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05164.1"
                     /translation="MSRIMFDAISDGIALTRRNLLKLCGASIGAYVSHCLPIRTVAKT
                     SPNILFILSDDHR"
     gene            4698..5318
                     /locus_tag="PLJ75_07860"
     CDS             4698..5318
                     /locus_tag="PLJ75_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPD05165.1"
                     /translation="MELRAQLIEFILLTLTVSLSGVMAPGPMSAVAMNHGTQQRFAGL
                     FIASGHAFVELPLIGALFFGIGFISRFSSLTAYITLAGSIVLFILGTVTIRDIRKQIT
                     IAPHSPLIDGIALTAFNPYFFIWWFTIGLSLIQKSVVFGLYGLVIFSILHVACDYVWL
                     FLLSMVSFAGASFLGKQFKTAVSIITVVMLYSFGAYFLYDALRQIL"
     gene            complement(5419..5826)
                     /locus_tag="PLJ75_07865"
     CDS             complement(5419..5826)
                     /locus_tag="PLJ75_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HPD05166.1"
                     /translation="MENIIKDPIVYAREVVGRDPLATFLGITVEEVKHGYARCAVTVK
                     PEYCNAVERAHGGIIHAVADQAFAVASNSMGTMAVALSMTLQYLQAALVGERIFAEVI
                     VIHAGRKVSSWRVDVRGVDDKIIATGDAVAYHK"
     gene            complement(5935..7191)
                     /locus_tag="PLJ75_07870"
     CDS             complement(5935..7191)
                     /locus_tag="PLJ75_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05167.1"
                     /translation="MKKALMAFCDSDGAIYKHPAAIAAFRSGRRFVAVEQEELMPLPD
                     GSTLYVLPGRFPVEGRSFKSVSNFNGNEILAVAAFLSSGYLRTYLPAYIKTPDAPLLS
                     LWAYCGVVFVGDDFYVPAMRIDPDQRSDPALHRNDGELFKTIKTIKKEYPGNSLVKQL
                     ITCSTKYGCLCARNFFLSRYEAPIPTTRMCNALCLGCFSFQKDTGIHVSQYRLTKEPS
                     PEEIAQVMLHHIERVDRAVVSFGQGCEGEPLMRAKDIARAITLVRKRTNRGTIHMNTN
                     GSKPEALTMCIDAGLDSVRVSLNSPTEEYYTRYYRPKNYYYNDVLQSIALACKAGIFV
                     SLNLFFLPGFTDAESEVKNLFSFLDRFPVDMIQTRNLNIDPDYYLDTISFVDEPALGI
                     RTLLATLRERYPDIRLGYYNPPKESF"
     gene            complement(7272..7784)
                     /locus_tag="PLJ75_07875"
     CDS             complement(7272..7784)
                     /locus_tag="PLJ75_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05168.1"
                     /translation="MNDKFHALTESLLTQEELQHCILLLFCLGDSSCDDPLLEELQIR
                     GYIVKEKDNRIITQKGKQYLSGLMQKLAQFHNAGEIIFQHYVDRELSPIKKEIHEIRM
                     QWRAVRDKRLARKAELQKMGKDKSALQKDSIYKALQKEQNALRTHLKHTEKKYHKKLA
                     NHVKNVGMLP"
     gene            complement(7781..7978)
                     /gene="thiS"
                     /locus_tag="PLJ75_07880"
     CDS             complement(7781..7978)
                     /gene="thiS"
                     /locus_tag="PLJ75_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HPD05169.1"
                     /translation="MKIRVNGIDIEFEKKTLKELLDLYRFNPSKIVVEKNGRIIDKEQ
                     YATEVLKDGDVLEIARFVGRG"
     gene            complement(7991..8713)
                     /locus_tag="PLJ75_07885"
     CDS             complement(7991..8713)
                     /locus_tag="PLJ75_07885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPD05170.1"
                     /translation="MAQFFVDSAGSDNSIIITGKDFYHLTTVRRASIGDAIQIIDNNG
                     MQYHATITQIDDGKIVATITEKLHYPDIHVQCTLCAALLKGKKFDLVIQKAVEIGVKQ
                     IIPVVTQHTIPDVSGKEDKKVERWQSIASEASKQCLRKEVPAVHNVMPFEDVIRLPKE
                     IGIMAHADPKAINLRNFLKEQVKHNNVMLLIGPEGGFSKKEVEIARQYDWFVLYSGAT
                     QLRAETAAIVLPAIILYEWGYE"
     gene            complement(8716..9492)
                     /locus_tag="PLJ75_07890"
     CDS             complement(8716..9492)
                     /locus_tag="PLJ75_07890"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPD05171.1"
                     /translation="MKQIKYLVVSIICYGVVVSTNAILYAQAEREIELTAQEILARVD
                     RIMQYPDGILKGYIMYITPDGSSQKLQITGYIANNQFLFDVSKPARGSMQKILYRIGG
                     EDLWVYDMAAKALYHKMGVDKFDDILATNFFYTDLSNADLQSNYVATIDGSVIIKNND
                     CYKLTLDPIDKTGIYGKLTLYVVKKTYIPLRIDYHDRDKVIFKTLSITKVAEKDEKSF
                     PVRYDMLDIKKGTMTILEFNSIEDGVRVDAELFRPEKLGD"
     gene            complement(9547..9939)
                     /gene="aroH"
                     /locus_tag="PLJ75_07895"
     CDS             complement(9547..9939)
                     /gene="aroH"
                     /locus_tag="PLJ75_07895"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPD05172.1"
                     /translation="MLRGVRGAITVDENTKEEIVSRTKELLQAIVTVNDIHTDDIASA
                     IFTVTDDLNAEFPAVAARTIGWIHIPLLCSREIPIPGSLGMCVRVLLHINTNKKQDEI
                     VHVYLRDAKKLRPDIGTPEQDKYYLSHD"
     gene            complement(9939..11210)
                     /gene="aroA"
                     /locus_tag="PLJ75_07900"
     CDS             complement(9939..11210)
                     /gene="aroA"
                     /locus_tag="PLJ75_07900"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPD05173.1"
                     /translation="MQFKVLPSSLKGEVVIPGSKSHTIRACVCALLANGTSTIKMPLD
                     SSDTRSCLGMIQRFGATVTQHNDEWVVTGCGIPAVPDDIIDIGNSGTSLYFGIGVASL
                     VNGITVFTGDNQIRNRPAQDLLTAITELGGWAVSTRSNGKPPVIVQGPITGGHVAIRA
                     ITSQYVSSLLIATPMAKNNTIIDVPLLNEAPYVGMTLWWLNELGIEYHANGYDQFIIR
                     GGQTYKPFSKYIPADFSSATFFMVAAAITGSTVKLKGLDINDTQGDKEVVTILKQMGA
                     DIAILPDAIVITGKPLKGGEFDLNAIPDALPALSVCGCFAEGETRLVNVPQARVKETD
                     RIAVMCAELKKMGADIEELPDGLVIRQSQLKGAPVQGHHDHRVVMSLAVAGLAAQGHT
                     VIDTAQAVDVTFPNFVELMLQLGAAIEREES"
     gene            complement(11516..11698)
                     /locus_tag="PLJ75_07905"
     CDS             complement(11516..11698)
                     /locus_tag="PLJ75_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05174.1"
                     /translation="MKVKKLRCEHCSLRATFDRSPRSLIGRLWRWHITWCPGWKAYSN
                     SLTEEERGELIAKYSL"
     gene            complement(11904..12431)
                     /locus_tag="PLJ75_07910"
     CDS             complement(11904..12431)
                     /locus_tag="PLJ75_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05175.1"
                     /translation="MRATHLIVTITFVLMSYVSVQAKTVLDYYCLIPDEFFQCEDDVK
                     ITRNYKLNQIKYKNIKAGYIRAESERNPMEVALFKDIKSNKDVVAVNIKCGPGCMCNR
                     FALLQFDANGKAINITASIFPNENLIWKKIKRTDVDAYEFILPEVGTTIKVVHADTGE
                     ELAQIFWRDGKFVLQ"
     gene            13045..>13964
                     /locus_tag="PLJ75_07915"
     CDS             13045..>13964
                     /locus_tag="PLJ75_07915"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPD05176.1"
                     /translation="MNATDIITSIGGIGLLLLGIALMSESIQKLAGSRLRIILTNLTG
                     GPGKALLTGMLITTIIQASSATAIATIGFVSAGLITMSQALAVIFGANVGSTVTGWLV
                     AIFGVQYSIGNISLILVAIGSILKILYTDKKSDIGMIMAGFGLFFFGISLMQQGMSQL
                     SSIINFSNIAGEGIFDRLILFAIGFVLTILMQSSSASVAIALIALNTGTIHLFQAQVL
                     VIGTNAGKSIPILFAGIGATPDTKRIIAADFLFNIITAIILFIIIPWLSPLLAFLFTT
                     IYPMPYEIQLALFHTLFNCIGILLFTPFITP"
     assembly_gap    13965..14064
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14065..14684)
                     /locus_tag="PLJ75_07920"
     CDS             complement(<14065..14684)
                     /locus_tag="PLJ75_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD05177.1"
                     /translation="MYQIISSSFQSNDCLLDARLYLPESNQKLPIIIMAHGFGARMDF
                     GLHPFADVFANMGFAVLMFDYRGFGKSKGSQQQLVYHKYHIEDYHSAIHYVKTTPNID
                     GAKIVLWGTSYSGGHVLTVASQDTTIAGVIAHVPFVDGIATAFHLPIKNIIIGMYSGV
                     RDMVTHIVNSKPYTIPIVSPPDSFAAMNTADSYQGYMNLVPAEVTD"
     gene            complement(14770..>16170)
                     /locus_tag="PLJ75_07925"
     CDS             complement(14770..>16170)
                     /locus_tag="PLJ75_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD05178.1"
                     /translation="VLFMVVSVHGFAQNNDAILSYDDYVSIMIKQLPQIKNNIIQVKK
                     AQVNVEKAKSVDDTTLVAQGQYTHQKQYTTGKNLFEPDYSETYYGYAGIERIIAPTGT
                     RLSAGFEYDASSVKGSRTIPAQAMSIDEYQPSIKATITQPLLKNAFGIADRYGKNNAE
                     YTLQIQKLQQLEDDNSVMNYYKKLYFTWIFYKQAIDILAESMANAKNLVVTVERKAKA
                     GLAENDDLQRAYSSLYSYQADYQQYQITYRALLDELWLYINKEATPDSAYLQKYFDEI
                     VTMDIQEIPFEKTRAYAIVAKSIDSMHYTIDVANNDTLPQMDLVLSASRKNYSQNQSD
                     ALSKLPDTDYSIGLQFSYPLGNHTSQSQLQEYELSLKELEHNLNISKNDYYKNIRTIL
                     QSLKGYKDLIEINTKNLYALVSQRQTERKKYEQARLDLKYLIETENSIFSQQLKLLQL
                     KVSFIDYFIDYNDLIQ"
BASE COUNT         4812 a         3347 c         2710 g         5201 t
ORIGIN      
        1 ttatcttgat ttattatata tgagtttgca ttctgtctca agctaacaaa ttagggttta
       61 atatgtatga aaaatatcct tctttattct gaaactttag cacaggttga atattcccca
      121 acacatcctt ttaaaccaat gcgtgcaaag cagatgctcg atcttttaaa cagatatgga
      181 ttattggaaa ataccgaaat aatagaacac cctgtccttg acgaagaact actatatctt
      241 ttccatacac ctgattatat acatgctctt aaaacatgtg accatggtga atttgatatt
      301 aacctcttga cctatggatt gggcacacat gataatccta tctttaaagg ggtatacaat
      361 ttttcattac aagcctcagg tggtactttt ttaggagcta caaaattagt tgatactgat
      421 gctacttttg ttttcaatcc aattggtgga tttcaccacg caatgaacga tcatgccgaa
      481 ggtttttgtt atatcaatga tatagccata gcattaaaac accttataca caatggtttg
      541 cgctgcgctt atattgatat tgatgttcat catggtgatg gcgttcaaag cgcattttac
      601 gatgataacc gggtacttac tatttcaatc catgaaagcg gacaattcct tttcccggga
      661 acagggtttg aaaatgagat tggtgaaggc gcagggtatg gttataatgt caatatacca
      721 ctgttaaagg acaccgatga cgaaatatat atctatgcat ttgaagaaat tgtcccacca
      781 ttattgcaaa gttttaatcc agacattatt tgtattcagg ttggcggcga tagtcataag
      841 gatgatcctt tagcccatct caacctcacc agcaacggat atcaaaaagt gctaacgctt
      901 ataaaacaac agtctaaaaa aatattggga acaggcggag gaggctataa tctgtataaa
      961 acggcagcgc tgtggacttt agcatggtca atttttaccg gcataaaacc aatggataat
     1021 tttatgggtg ttattggcgg aatgatgtat ggacctgaag ctgatgctgg ttctctattc
     1081 gacaatccct attctgttgg tggattaaca aaggataaat gctttgacta cgcacgcgat
     1141 gttgttgctt acattaagca atctgttttt cccattcata acatctgatt gttgctataa
     1201 tcgttttatt gttgcctcca caccgttgag tgccaccagt attgatgcat aataccctct
     1261 tttcagacta ccggtattga ccactgtagt gtggcccatt ttcccgatac cacacgcttc
     1321 atgaatatgg ccgcatatgc ataagttaat gttatattca caacaaaatt gtgcaaccga
     1381 gttgcttccc gcatgcgttt tgaatagtgt cctgtcacac atgttcttag gaggggtgtg
     1441 tgataccagc acctttacag gaagatggtt aatgtgttca aaacctttat acaagtaatc
     1501 atgtatttgc tcttcggtat attctatcgg agtggttaat ggcgttttta ttgaaccacc
     1561 taccccaaaa aatcctacat catccactat tgcaaaacct gcatggattg agtagggcga
     1621 tagaaattct tgaatcaggc tgccgtccca attgccatgt acagccagca caggtttatt
     1681 aatccacgcg atagttcttt tatatgcatg ccagttagta taccacatca caggtccaat
     1741 atcaccagca ataacaacaa tatcgcttga ttcaatctcg ggctgtaagc gataaatatt
     1801 ttcataggct ctgtgtatat cactgaaggc gcaaattttt atcattattt actgtccaga
     1861 tttgaaattt tgatagcaaa ccataaatag aatgaaaaag cggggcaaat atagtaaaag
     1921 tcaagaatta tatatagaat atatctttaa gaaagttttc ctctttgaag caatatttaa
     1981 actatattcc cctatcctcg tcaaatattt ctgccggaat caacacacca aaatacttta
     2041 tagcagaaac aagcctgtca cattcatcaa cctggggtaa tattgcacat tcaaacggtt
     2101 cttcatactc cttataaatg tataattgtg catttttcat atagataacc ctgcatttaa
     2161 taatcgtatt ttaatatagc ataaattaca ttaaaatcaa gttgattaaa caattttata
     2221 ttaaaatata tttaaatttg tatttacaag attatcgata ttttttaaag gcttattaca
     2281 atgaccggga aatgttatat gtataattta agaataaagt gagggagtgt acatggccgg
     2341 cgtatgatca gccataatgg atgaaaatta tacaatccgg aaacaatact ccccttgctc
     2401 tatgaaggtg aatggccggg gtttgaggtt aatatatcta tctccggagg aagtatatat
     2461 aagcataaat agtaatagaa atattaatga aaaattaatt tatatcctgt tacataaata
     2521 taaaaccagt gttcgccaac acctcatatc agaaaaacat aaagagcaat aattccggtg
     2581 acaacagaca tgactggcaa aggtgtaatt attttttgta attcaattcc tttctttttt
     2641 aatctatatc gacagaacat ctcactaatc cccatgaaag caaaagaacc agatagtgct
     2701 aataaaaacg ctgaattaaa tgtataatat aatgtttccc caaaggtaaa aaattcactt
     2761 acccttacaa gttcttttat aatccacact atctgtacaa ttaatgacac aacaattctt
     2821 tcatgtatag ttgcaccttt gaaatataat acaggataaa ctataagagc attaaagcca
     2881 ataaatacaa ataacacaat accagctata aaagagatcc agtactgtac atctctatta
     2941 tcaattttcc atgattcaaa gtaaagattg tgaagcacta tgtaaataac aagcagtaat
     3001 actattggtg taataaaccg cgacacaaag ttttgcttta ccacatagct gtgttcaacc
     3061 ctgtgttcaa tcatattgtt ttcccatctt taaatcttct aacaattttt taagttccgg
     3121 tttaagcttt aatccttttt gtgtatttaa aagattatcc ctttcatcag gatcatctat
     3181 aacattatag agttctggct tccgccttcc accatattcg atgtaaatgt gtgaatcagt
     3241 tcggacagca cgaatttctg gaacattata gggaaattcc ttaaaatact catagagcca
     3301 tgcttttctt ccataagctt tatcagacag caatattggc tttaaacttt ttccttctat
     3361 atgtggtggt atagaaatac ccgctaaatc aagtaatgta ggtgccagat caatatttaa
     3421 taccatttga taagcctttc tcccgggatt tttaataaat ccaggatatc ggacaataag
     3481 aggtattcgt atggcttctt cataagccca tcttttatca atcaaacgat gctctcccca
     3541 gaagtatcca ttatctcctg catagataac aacagtgttt tcctatattc ttgattcatc
     3601 taacgctttt agtattctgc caatctgcct gtcaagtgct acaacagttc tgcaataatt
     3661 gagataatgg tgttccaatg taccaaacgt acccgcccat gttccaccct gtactgttgt
     3721 tacccacgag tcagattctt ctggcaataa ttgttttaca tcaacgtcat tatataaatt
     3781 ttttaaatca tccggcgaca ggaaaagctg atgaacagct ttgtatgaca gatataaaca
     3841 gaatggattg tttcgtttag ttttaataaa ttctattgca aaatcggtta aatcatcagt
     3901 aatatattta cctttacgtt ctgttaaaac gccatcaatg tatagcggac aatcaaaata
     3961 tcttccctga ccttcctgaa tggtaaaaga aataaatcta tcaacacctc taagttttgg
     4021 gagccctttt ccgggcatgt gctatttccc aataaaagca gtatcataac ctgcttgttt
     4081 taatatttct aaagatgtta tattttcatt attccataca gtcaggttgt ttttaacacc
     4141 atgggtgtga gcatattgtc cggcaagaaa gcttgctcgc gaagggctgc aaagtgatgt
     4201 tgtcacaaaa gcatttttaa aatgtactcc ttcagatgaa agcctatcaa gatgtggggt
     4261 cttgattata gcatgcctca tacagctcat agcatctcag cgatggtcat cagaaagaat
     4321 gaatagtata tttggacttg tttttgctac tgtacgaatt ggcaaacagt gtgaaacata
     4381 cgctccaatt gaagccccac atagctttaa aagatttcta cgtgttaatg ctataccatc
     4441 agatattgca tcaaacatta ttctggacat tgccatccac ctttttgtat agaggatatt
     4501 taaagcaatg gcagacagct taatactata atacttcaat ggtaatgtca atgataatgt
     4561 tgaaaattaa tcttttcatt gtatagcagc attaaagttt tacaacgcta ccaatagcat
     4621 actgtaaaaa atatatgcaa cgccagaaaa tgcttgaaat tggcatatca tgaatcaatc
     4681 tggtacagga tacaaccatg gaattaagag cacagctcat tgaatttata ctgcttaccc
     4741 tgaccgtttc actttctgga gttatggctc cagggcccat gagtgctgtg gcaatgaatc
     4801 atgggacaca acaacggttt gccggattat tcattgcaag cgggcatgct ttcgtggaac
     4861 ttccgcttat aggagcactc ttttttggga taggttttat ttcgcggttt tcctctctta
     4921 cagcatatat tacccttgca ggcagcattg tattatttat ccttggcaca gtaaccatac
     4981 gggatatccg taaacaaatt actatcgctc ctcattctcc ccttatcgat ggtattgctc
     5041 ttacagcctt taatccatat ttctttatat ggtggtttac tattggtttg tcgcttattc
     5101 aaaaatcagt ggtatttggt ttatatggat tggttatttt ttctatactg catgtagcat
     5161 gcgattatgt atggttattc cttttatcaa tggtatcatt tgcaggcgct tcatttttag
     5221 gaaagcaatt caaaacggct gttagtatca taactgttgt tatgttatat agttttggcg
     5281 cttacttcct gtatgatgcc ttacgccaga tactttaaga tatttcacca tcatctatgg
     5341 tgtcgccttc agtgattata caagccatat aatcaacact atggtacgtc agcatcatac
     5401 catgcagcaa cagcaaattt atttgtgata ggctacagca tctcccgttg caataatttt
     5461 atcatctaca cctcgtacat caacacgcca tgaacttact tttctaccag catgaattac
     5521 aataacctct gcaaatatac gttcgccaac cagtgcagcc tggagatact gtaatgtcat
     5581 actcaaagca acggccattg tacccattga attgcttgca acagcaaatg cctgatctgc
     5641 cacagcatga ataatgccac catgcgcgcg ttcaactgca ttacaatatt caggtttaac
     5701 ggtaacagca caccgcgcat agccatgctt aacttcttct accgttattc ctaaaaatgt
     5761 agccaatggg tccctgccta cgacctcccg tgcatacaca attgggtcct taatgatatt
     5821 ttccatttac atcactccct cagaagataa cattatttct caaagtcatt ccgggcttga
     5881 cccggaatca aaataattac aagatttcct taattgccac aggattcaca tccactaaaa
     5941 actttctttg ggcggattgt aatagcccaa ccgtatatca ggataccgct cccgcaaggt
     6001 agcaagcaac gtacgaatac caagcgcagg ttcatcaaca aaactaatcg tatctaaata
     6061 ataatccgga tcgatattca gattccgtgt ctgtatcata tcaacaggaa atctgtcaag
     6121 aaatgaaaaa agatttttta cctcactctc tgcatcggta aaacccggca aaaagaaaag
     6181 gtttaatgaa acaaatatac cagctttaca ggccaatgca atactctgta atacatcgtt
     6241 ataataataa tttttagggc gatagtacct ggtgtaatat tcttcagttg gggaattaag
     6301 gcttaccctg accgaatcaa gtccagcgtc aatacacata gtaagagcct caggctttga
     6361 accattggta ttcatatgaa ttgttcccct gtttgttctt ttacgaacca gcgttattgc
     6421 ccgtgctata tcctttgccc gcataagcgg ttcgccttca caaccctgtc caaaactaac
     6481 aaccgcccta tcaacccgtt caatatggtg cagcattacc tgcgcaatct cttcgggcga
     6541 tggctctttc gtgaggcgat attgcgaaac atgtataccg gtatctttct gaaatgaaaa
     6601 gcaacccaga catagcgcat tacacattcg tgtggtgggt ataggtgcct cataacggct
     6661 taagaaaaaa ttgcgtgcac ataaacatcc gtatttagtg gaacaggtta ttaactgctt
     6721 aaccaggcta ttgccgggat attccttctt aatagtcttt attgttttaa aaagttcacc
     6781 atcattacga tgaagggcgg ggtcagagcg ttggtcagga tcaatacgca ttgctggcac
     6841 ataaaaatca tctcctacaa aaaccacccc gcagtacgcc cataatgaca atagtggagc
     6901 atcgggagtc tttatgtatg ctggtagata tgtccttaaa tagcccgagc ttaaaaaagc
     6961 agccacagcc aaaatttcat tgccattgaa attgctcaca gacttaaacg accttccttc
     7021 aacggggaac cgtcccggca atacatacaa tgtactccca tcgggaagcg gcatgagttc
     7081 ttcctgttca acggcaacaa accgcctgcc gcttctaaat gctgcaatgg cagcgggatg
     7141 cttatagata gcaccatcac tatcacaaaa tgccatcaac gcttttttca tacaatcgtt
     7201 ttacaccgca ttcaataata ttaatagtat cactgcatca ccattgagta tgcacccttg
     7261 catgatgtat ttcaggggag cattcctaca ttttttacat gatttgctaa ttttttatga
     7321 tacttttttt cggtatgttt caggtgtgtg cgcaatgcat tctgctcttt ttgcagtgct
     7381 ttataaatac tatccttttg caaagctgat ttatccttcc ccattttttg caattccgct
     7441 ttacgcgcta atcgtttatc acgaacagcg cgccactgca tgcgtatttc atgtatctct
     7501 ttttttattg gcgatagttc cctgtcaaca tagtgctgga aaataatttc acctgcatta
     7561 tgaaattgtg ccagcttttg cataagccct gatagatatt gtttgccctt ttgagtaatg
     7621 atacgattat ctttttcttt gacgatatag ccccgaattt gcaattcttc aagcaatggg
     7681 tcatcacatg agctatcgcc caggcaaaac aacaacaaaa tgcaatgttg cagttcttct
     7741 tgtgtaagca aagattccgt cagagcatgg aatttgtcat tcatcctcta ccaacaaatc
     7801 gcgcaatttc aagaacgtcg ccatctttca aaacttcagt tgcatactgt tctttatcaa
     7861 tgattcgccc atttttctca accacgattt ttgatggatt aaacctgtaa agatcaagaa
     7921 gctctttaag ggttttcttc tcaaattcaa tatctatccc gtttaccctg atcttcatac
     7981 tactatcctg ttattcatat ccccattcat ataaaatgat tgccggcaaa acgatagctg
     8041 cagtttcagc tctgagctgt gttgcaccac tgtatagtac aaaccaatca tattgtcgtg
     8101 caatttcaac ctcttttttt gaaaaccccc cttcaggtcc tatgagcagc ataacgttgt
     8161 tatgttttac ctgttctttt aaaaaattcc gtaagtttat cgctttgggg tcagcatgtg
     8221 ccataatacc aatctctttt ggaagcctta tgacatcttc aaaaggcata acattatgaa
     8281 ccgctggcac ctctttgcgt aagcattgct ttgaggcttc gcttgcaatt gactgccagc
     8341 gttcaacctt tttatcttct ttgcccgaaa catccggtat ggtgtgctgc gtaacaacag
     8401 gaattatttg ttttactcct atttcaacag ctttctggat aacaaggtca aactttttgc
     8461 cctttaacag tgcagcacac aatgtacact gtacatgaat atcaggatag tgaagtttct
     8521 ctgttattgt tgcaactatc tttccatcat ctatttgtgt gatggttgca tggtattgca
     8581 taccgttatt atcaattatc tgtatagcat cacctatgct tgcgcggcgc accgttgtta
     8641 gatgataaaa atcctttccc gtgatgatga tgctattatc actgccagca gaatcgacaa
     8701 aaaactgtgc caccatcaat ctcctaactt ctccggtctg aataactctg catcaaccct
     8761 gacaccgtct tctatgctat taaattcaag tatcgtcatt gtaccttttt tgatatcaag
     8821 catatcatat cgtacaggga atgatttttc atccttctcc gctacttttg ttatagacag
     8881 tgttttaaaa ataaccttat ccctatcatg gtaatctata cgcaatggga tataggtttt
     8941 ttttaccaca tataaggtta attttccata gataccggtt ttatcaattg gatcaagggt
     9001 tagtttgtaa caatcattat tctttataat cacagagcca tcaatcgttg caacataatt
     9061 gctctgcaaa tcagcattgg acagatcagt ataaaaaaag ttggttgcca gtatatcatc
     9121 aaacttatca acacccattt tatggtatag tgctttcgct gccatatcat atacccacaa
     9181 atcctcaccg ccaatccggt acaatatttt ttgcatgcta ccgcgtgcag gtttgctgac
     9241 atcaaataaa aactgattat ttgcaatata ccctgtaatc tgtaattttt gcgatgaacc
     9301 atcaggtgta atatacatga tatatccttt taaaatgcca tcaggatatt gcattattcg
     9361 gtcaactcgt gccaatatct cctgagcagt cagttcaatt tcacgttcag cctgtgcata
     9421 cagaatagca ttagtgctta caacaactcc atagcatata atcgacacta ctaaatactt
     9481 gatttgcttc attgaatgta cccggtacat attattttta tttagtattc atctgcacaa
     9541 ttcttctcaa tcgtgcgata gatagtattt atcctgctca ggtgtaccaa tatccggtcg
     9601 caacttttta gcatcgcgca gatatacatg cacaatctca tcctgttttt tattggtatt
     9661 gatatgcaaa agaacccgta cgcacatgcc cagtgaacca gggattggta tctctcggga
     9721 acataaaagg ggtatatgaa tccagccaat agtacgggca gcaactgcag gaaactcggc
     9781 attcaaatca tcagtgacag taaatatggc tgatgctata tcatcagtat ggatatcatt
     9841 gacagtaact atcgcctgca aaagctcctt ggtccttgat actatttctt cttttgtatt
     9901 ttcatcaacg gttattgctc cacgcactcc cctgagcatc atgactcctc cctttcaatt
     9961 gcagcaccca gttgcagcat aagttcaaca aaattaggga aggtaacatc caccgcctgc
    10021 gctgtatcaa taacggtatg accttgtgca gccaatccgg caactgcaag cgacatcaca
    10081 acacgatgat catgatgtcc ctgtacggga gcacctttta attgactttg cctgatgaca
    10141 agcccatcag gaagctcttc tatatcagca cccatttttt ttaattcagc acacatcact
    10201 gcaattcggt ctgtctcctt aacccgtgcc tgtggtacat tcaccagccg cgtttcgccc
    10261 tcagcaaaac atccgcatac tgacagtgca ggaagagcat ccggaattgc attaaggtca
    10321 aactcgccac ctttaagtgg cttaccggta ataacaattg catcgggaag tattgcaata
    10381 tcagcgccca tctgctttag aattgtaacc acctctttat cgccctgggt atcattaata
    10441 tctaacccct taagttttac cgtgctacct gttatggcag ccgcaaccat aaaaaacgtt
    10501 gccgatgaaa aatcagcagg gatgtatttt gaaaatggtt tgtaggtttg acctccacgt
    10561 ataataaact ggtcataccc attagcatga tattcaatac caagctcatt aagccaccac
    10621 aatgtcatgc caacataggg agcttcattg agcaggggca catcaatgat agtattgttt
    10681 tttgccatcg gtgttgctat aagcagtgat gatacatact gtgaggtaat tgccctgatt
    10741 gcaacatgcc ctcctgttat aggtccctgc acaataaccg gcggcttgcc attactacgc
    10801 gttgaaactg cccagcctcc taattctgta attgcagtaa gcaggtcctg cgcaggcctg
    10861 ttgcgtatct gattgtcgcc ggtgaatacg gtgataccat tgacaagcga tgcaacacca
    10921 attccaaaat aaagcgatgt ccccgaattg ccaatatcaa tgatatcatc cggaacggca
    10981 ggaatgccgc aaccggtaac aacccattca tcgttgtgct gtgttaccgt tgccccaaac
    11041 cgttgaatca taccaaggca ggaacgtgta tcacttgaat caaggggcat ttttatggtt
    11101 gaagtaccat tagcaagaag agcacataca caggcccgta tggtatgcga tttagaaccg
    11161 ggtataacga cttcaccttt taatgaagat ggcaaaactt taaattgcat actttacact
    11221 tatttcattt tattgatttg taagtgacaa tttaattaat ccaaaccaaa ttttgtcaat
    11281 agattttatt gaataattgt taaatagtga ggattttacc agtatttttt atcttaaatg
    11341 tgaataatgg tatagtaata cttatcctgt cagattttga cgaagacact gtgaaagctc
    11401 aatcaattgt tatttcacca taaacatcac aataattaaa aaagactgct tcttcaaaaa
    11461 tccatggatg tgcaacgtat aaaaactata ggcaatatgt ttccctattt tatatttata
    11521 atgaatattt tgctatcaac tctcctcgtt cttcttcagt taaagaatta ctatacgctt
    11581 tccatccggg acaccatgta atatgccagc gccatagtct gccaatcagt gaacgaggac
    11641 ttctgtcaaa agttgctcgt aacgagcagt gttcacatcg taatttcttt actttcatcg
    11701 ttttctccag ttgttccatt ataattaaga gcgatagtta ctgactattg attgagtaca
    11761 tttcattgag ctgattggca acaattgagg tgttttcgcc cctcagagaa gattatctcc
    11821 cctgattgtt atacgcccag tgcaacaccc ttaacacaag gtgttgtact taatacttaa
    11881 ccataacttt tttatttgtt tttctactgt aacacaaatt taccatcacg ccagaaaatt
    11941 tgtgccaatt cttcacccgt atcagcgtgt acaactttaa tggtggtacc aacttcggga
    12001 agaataaatt cgtatgcatc cacatcggtc cttttaattt tcttccatat caaattttca
    12061 ttgggaaaaa tagaagcagt gatattgata gccttgccat ttgcatcaaa ctgtaaaaga
    12121 gcaaaccggt tgcacatgca acctggtccg cacttaatgt tcacagccac aacatccttg
    12181 ttgcttttta tgtccttaaa caatgccact tccatgggat ttctttccga ctcagcgcga
    12241 atgtaacccg ctttgatatt cttgtatttt atctgattga gcttataatt gcgggttatc
    12301 ttgacatcat cctcacattg aaaaaactcg tctggtatta agcaataata atccagaact
    12361 gttttagcct gaacagaaac atagctcata agtacaaatg ttatcgtaac aataagatga
    12421 gttgcacgca tttttcgttt cctccatttt aattaatcta tattttgaat caaaaatata
    12481 aaaagcttat tcaaaaaaca ttgttctatt gagtaaaaat atgttcacga tatgattgct
    12541 tataacagta tatacaatct ttagaaagac atcgttgatc tggcttccct catttttacc
    12601 aataatcttc gttaataatc accaacatat cgacccaata ataatcaggt tcactgaatt
    12661 ttatctcttg atttccaaac tgcatttatc acatagaatt tcttatttga ttttaatata
    12721 agattaaaaa tgaagcggaa gtcaaggaat tttttcaaat ttttatttat aattgcgtaa
    12781 cttcaaacac acctgattat agtatcgaga gtaatttctt atgttgccca tttttcacaa
    12841 agttatatct tgtgttgtca ataatctcaa ctgcattttt taagcatttt catctctttc
    12901 aagccttgtt tacatcttaa ttatgagtgc attcatattc ttgaacaata aatataacat
    12961 tataaaaaat aaatcttgct ctaaatacta tctttcatat aactatccta taattgccga
    13021 tactatagta aaaagagtta ccgtatgaat gcgactgata taataacatc cattggcggt
    13081 ataggactgc tccttttagg tatagctctc atgtccgaat ccatacagaa actggcaggt
    13141 tcacggctgc gtattatcct taccaacctt accggaggtc caggcaaagc actccttacc
    13201 ggtatgctca ttacaaccat tattcaagcc tccagtgcta cagccattgc cactattggt
    13261 tttgtaagtg ctggactcat taccatgtca caggcacttg cagtaatctt tggtgcaaat
    13321 gttggctcaa cagtaacagg ctggcttgtg gcaatctttg gcgtgcaata tagtataggg
    13381 aatatatctc ttatcttagt tgctataggc agcatattaa aaattcttta tactgacaaa
    13441 aagagcgata tcggcatgat catggcaggt tttggattgt ttttttttgg tatcagcctt
    13501 atgcaacagg gcatgtcaca actgtcatca atcataaact tttcaaacat tgctggagaa
    13561 ggcatttttg accgattaat actttttgct attggctttg tactaaccat tttaatgcaa
    13621 tcctcaagtg cttctgttgc aatagcacta atagctttaa atacgggcac cattcatctt
    13681 tttcaggcac aggttcttgt cattggaacg aatgcaggga aatctatccc aatactgttt
    13741 gcaggcattg gtgcaacacc tgatacaaaa cgaattatcg ctgctgattt tctttttaat
    13801 atcataacag caatcatatt atttattata ataccatggt tatcgccatt gcttgcattt
    13861 ctatttacta ctatctaccc aatgccttat gaaatacagc ttgcattgtt ccatacatta
    13921 ttcaattgta ttggcatatt gctgtttaca ccctttatta caccnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnntcatca gttacctctg ctggcactaa attcatataa
    14101 ccctgatagc tatcagcagt attcatagcc gcaaaagaat ctggcggcga tacaatgggt
    14161 atagtatagg gcttgctgtt aacaatgtgt gtcaccatat cccgaacccc tgaatacata
    14221 ccaatgatta tattttttat gggcagatga aatgctgtgg caataccatc cacaaatgga
    14281 acatgcgcta taactccagc aatagtagta tcctgtgatg ctaccgttaa aacatgcccg
    14341 ccgctatacg atgttcccca caatactatc tttgcaccat caatatttgg cgtggttttt
    14401 acatagtgta tggcactatg ataatcctca atatgatact tgtgatacac caattgttgt
    14461 tgactcccct tgcttttgcc aaagcccctg tagtcaaaca taagcacagc aaaacccata
    14521 tttgcaaaga catccgcaaa cggatgcagt ccaaaatcca tcctcgctcc aaatccatgc
    14581 gccattatga tgatgggcag tttttgattg ctctctggca ggtaaaggcg ggcatctaat
    14641 aaacaatcat tgctttgaaa cgatgaagaa ataatctgat acataacaat ctcctgaaac
    14701 attagaatgt catataatct ctgtaacaat gacgcgttta actatttata tacaattaaa
    14761 catacactat tactggatta aatcattata atcaataaaa taatcaataa aactaacctt
    14821 caactgtaat aattttagct gctgagaaaa aattgaattt tcagtttcaa ttaaatattt
    14881 aagatccagc cttgcttgct catacttttt tctttcagtc tgccgctggg ataccaatgc
    14941 atataaattt tttgtgttta tttctattaa atctttatat cctttcagcg attgtaagat
    15001 ggtacggata tttttatagt aatcattctt tgatatattc aggttgtgtt ctaattcttt
    15061 aagggatagc tcatattcct gtaactgtga ttgggacgta tgattgccca acgggtagct
    15121 aaactgtaac cctattgagt aatccgtatc aggaagtttg cttaacgcat cactctggtt
    15181 ctggctataa ttttttcgtg aagctgaaag aactaaatcc atctgtggca gcgtgtcatt
    15241 attggcaaca tctatggtat agtgcatgct atcaatactt ttagcaacaa tggcataggc
    15301 acgtgttttt tcaaaaggga tttcctgtat atccatagta actatctcat caaaatattt
    15361 ttgaagatat gcactatcag gtgttgcctc cttattgata taaagccata gctcatctaa
    15421 aagagctcta taggtaatct gatattgctg atagtctgcc tgatagctgt ataatgaaga
    15481 gtatgcccgc tgaagatcat cattttcagc cagccctgct ttagccttac gctcaacagt
    15541 cacaacaaga ttcttagcgt ttgccatgct ttccgctaaa atatcgattg cctgtttata
    15601 aaaaatccat gtaaaataca gcttcttata gtagttcatg accgagttat catcctcaag
    15661 ctgctgcaat ttttgtattt gcagggtata ttcagcattg ttttttccat atctatcagc
    15721 aataccaaat gcatttttta acaacggttg tgtgatagtt gccttgatgg atggctgata
    15781 ttcatcaatt gacatagcct gtgccgggat agtacggcta ccttttactg acgaagcatc
    15841 atattcaaac cctgctgata atcgtgttcc tgttggtgca atgatacgtt caattccggc
    15901 gtaaccatag tatgtttcac tataatccgg ttcaaaaagg tttttaccgg ttgtatactg
    15961 tttttgatgg gtatattgtc cttgagcaac cagtgttgta tcatcaacac ttttagcttt
    16021 ttcaacattt acctgtgctt ttttaacctg aatgatgtta tttttaatct ggggcaactg
    16081 tttaatcatg attgatacat agtcatcata cgaaaggatt gcgtcattgt tttgagcaaa
    16141 accatgcaca cttacaacca taaacaatac
//
LOCUS       DAOOJH010000052        15922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3550_length_15922_cov_8.407575, whole genome shotgun sequence.
ACCESSION   DAOOJH010000052 DAOOJH010000000
VERSION     DAOOJH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3550_length_15922_cov_8.407575"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLJ75_07930"
     CDS             <1..656
                     /locus_tag="PLJ75_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPD05179.1"
                     /translation="DEDLRCGVLFAHGDHFTGGLDLVQWVEPFSKGEYPALPAGSKDP
                     FGIDEDNRVSKPIVSALQGICFTVGWELMLATDVRVAASNVRLAQIEVKRGIYPVGGA
                     TVRLFEEIGWGNAMRYLLTGDEMNAQEAYRLGLVQEVVEPGKQLERAIEIATVIAKQA
                     PLGVRAILKSARLSRVEGAKAALERLLPDLLPIMKSEDAQEGVRAFIERREAAFKGK"
     gene            complement(668..1177)
                     /locus_tag="PLJ75_07935"
     CDS             complement(668..1177)
                     /locus_tag="PLJ75_07935"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPD05180.1"
                     /translation="MEIILFITALLFALGGILGSVLPILPGPPLSFFAVILLHWSGFA
                     SFSFTFFIITGIIAAILTVFDYVLPSWIVNKFSGSKQGVIGSMIGLIVGSIILPIIGI
                     IIGPFIGAFIGELVANNNALTALKAALLTFIGFLISTGLKLGYTITIAYYIIKALMGQ
                     PPAPDYPII"
     gene            complement(1262..1936)
                     /locus_tag="PLJ75_07940"
     CDS             complement(1262..1936)
                     /locus_tag="PLJ75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPD05181.1"
                     /translation="MKRVTYGLIAFIVCCSIGMLYAQDEKAKEAQKVLEQEKQLSDQI
                     YKEIVLEDFETVEYTDKNISLRVVKDEKAGVSMRTDYPAPIQNSKRYLGVKVFAKSGS
                     VLTIIPAKPLIIDKHCKYINMWVYGKNFSGELSIMVKDANGNIKRLVMGKLNFLGWRK
                     LSVPITDEIAQEDKYLAQKRNIEIVKILYNPSTLERLPAWNYFYIDDITAIVREKYID
                     KQSDEW"
     gene            complement(2077..3198)
                     /locus_tag="PLJ75_07945"
     CDS             complement(2077..3198)
                     /locus_tag="PLJ75_07945"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPD05182.1"
                     /translation="MIKILKKTCILFFAMGVIMTVDVSCKQASDRPKGFQSNAIKVMT
                     INVWSGLDYIGTFKMGEYETPQIREKRYQALLKEIANLNPDIIAINEANFLPDYMKRL
                     ASDLNYDYIYHVGVAGLKIGRLGIPTNLKEGDGLLARKQLQLQQVGHKKLSGGGIITN
                     CFSFHTQDATQVLEGRVIINEKPVFVAVTHWHASPPDTPQNRILLKELKETYGYSDDE
                     YTQAIQQLEADNKWRNNESIAMAEFLKKVVPADAPCIVMGDFNTTIDSPEMRYFLSQG
                     YHDTFLPSNNTSPYTWNPMENANIKKYYVTDTRTKFDSLYAHCTKINDLQPKTIDYIL
                     TNNVIPRTMIHSSTVCCTTLHDGYHPSDHFGVFAEILIQ"
     gene            3651..5210
                     /locus_tag="PLJ75_07950"
     CDS             3651..5210
                     /locus_tag="PLJ75_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05183.1"
                     /translation="MFIVKSSVLANGSFNWFWFIYEQGTYYDSTYTVYRPLYVKNVYR
                     DSSFSASLMPLIYWNYTTPNQDKTRGFLGFYVSIDYRSANHYENDTGVFPFVMYGTSN
                     DKEDQYFHLWPFGGTVKSKLGADAITTYAFPGFVLFILYPTTSYYVLAVYLVASMIPI
                     YAHYTIRDYDGHAIIWPLIRWGKSPQRDDFRIMPFYAHSKKKGYYSTASYGMLYNVRE
                     EYIGDDTMHTVFLVPFYARRWLDSGKADSSTLLWPFFSWGYNDKHGNVEINFPWPLVQ
                     IRDCDDPYIYKRIFFPFYGKYIEKRKTTFFISPFYFVLEKQKDSFHSQYSFYGILFWH
                     FQRDYKYGHKRYGNHWRFYKLWPLFHYEYNDKGDSSFAILSIFPFRDEEGYERLYQPF
                     FSLVEYATRDDGSKRFGLLLRTYFQSWGDSYFKMKIPFIISYHSKDKTVQHVSLLLSF
                     LEYTNDESINELRIGWIPVYRNVKDKKQYDDKKKVSLRLQQIEGGYNSMVAYGSTTSY
                     YNDSTGCMVRF"
     gene            5297..6103
                     /locus_tag="PLJ75_07955"
     CDS             5297..6103
                     /locus_tag="PLJ75_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD05184.1"
                     /translation="MIKKLRDVIVNVVGGVGKNTLHIVYMMGYSTMLFLEVVYYSRSA
                     FDKRKEILKQMYYAGVKTFIVVSIVALFTGMILALQIGLELKPYQQQPLVGNIIIATL
                     TREMAPFVTAIILIAAVGSTMAAEVGTMAVSEEIDALEMMSISPVRFLVMPRVISLAL
                     MLPIATVYTNLLGCIGGGIIAHFQLNVSFDVYYSHILESLHFKATYVGLLKAFIFGIM
                     VSTISCANGLRATNGALGVGKATRNSVVSSFLMVLIVGYYITGIFYGKAG"
     gene            6130..6876
                     /locus_tag="PLJ75_07960"
     CDS             6130..6876
                     /locus_tag="PLJ75_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD05185.1"
                     /translation="MIEFKHVTKILNGVKVLDNLNITIEKGETFVIIGRSGTGKTVTL
                     KHIAGLLEPESGAVLIDGKQMNGASRRQKDSLREKIGFVFQSGALINWMTVEENIALP
                     LVEHKVLSADEIKQIVEEKLDLLQLTSARDRMPAEISGGMKKRASLARVLVRNPEIIL
                     YDEPTSGLDPVMSSLINTIINQIKKDQHITQVVVTHDMESAYAIGDRIAMLYDGSIIE
                     CDVPDAIRTTTNPIVRQFISGSLEGPIEVL"
     gene            6968..7621
                     /locus_tag="PLJ75_07965"
     CDS             6968..7621
                     /locus_tag="PLJ75_07965"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPD05186.1"
                     /translation="MKQEMAVGIVVILAMTVLGYFTIIMSGEIIDTHTYYPASIVFKD
                     IEGLAKDDKVRINGVLSGYVAGTQLLDNKVIVTLKLFNHFTLYENYEIMIRNETALAG
                     KYVSIDPGTPVDENGKQYAVVTTRENLSGIAVSDPFTLLSRLIADNRGNVNSVLKNIR
                     DITDKINSGKGTLGKIINDATVHDQASGLIKELRDTIEDTREQAPITSFLRAALTAF"
     gene            7750..11091
                     /gene="carB"
                     /locus_tag="PLJ75_07970"
     CDS             7750..11091
                     /gene="carB"
                     /locus_tag="PLJ75_07970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPD05187.1"
                     /translation="MPRRTDITKILIIGSGPIVIGQACEFDYSGSQACKALREEGYTI
                     VLVNSNPATIMTDPEIADRTYIEPITHDIVAQIIERERPDALLPTVGGQTALNIAVQL
                     YDSGTLQKYGVELIGAKIEAIKKAEDRDLFKKAMLSIGLQVPRSVLASSIDEALTVLE
                     SISLPIIIRPAFTLGGTGGNIVYNQEDFIELVRRGLDESPISQVLLEESVIGWKEFEL
                     EVMRDLNDNVVIVCSIENFDPMGIHTGDSITVAPQQTLTDREYQVLRDMAIAIIKEIG
                     VETGGSNIQFAVNPDDGRIMVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGLTLDE
                     IRNDITRETPACFEPTIDYVVTKIPRWAFEKFEGADTTLGTQMKSVGEAMAIGRTFKE
                     SFQKALRSLEIERYGFGSDGNVKIDAMLDGLSENKKNDIIEMNLRIPKENRVFYIKKA
                     LELHWSVDTIYSLTSIDPWFLHQLKELVDAEHDCIAQLNSGLSKDIIVRMKQWGYSDR
                     QLAYLAHKDELTLLYAKGIDSQKDYSKRLSELEDDIRNYRQKNGIVPGYRLVDTCGGE
                     FEAYTPYYYSSYDDEDEARYSDKRKIMILGGGPNRIGQGIEFDYCCCHASFALRDAGV
                     ESIMVNSNPETVSTDYDTSDRLYFEPLTLEDIIHIYQKEKPEGVIIQFGGQTPLRLAQ
                     RLEANGVKILGTPPDAIDRAEDRERFAQVVKKLNLHQPENGIAFTQKQALEQANRIGY
                     PVLVRPSYVLGGRAMAIMYDEKSLIEYIVSAAELSPEHPILIDKFLDDALEIDVDALC
                     DGMDVYVAGIMEHIELAGIHSGDSACSLPPVSVKPVMMHTIMATTAALAHELGVIGLL
                     NIQFAIKNDVLYVLEVNPRASRTVPFVSKATGVPLAKIAVQILLGKKLKDFGLTQANT
                     MPYCSVKEAVLPFNKFPGVDTLLSPEMKSTGEVMGIANNFGEAFYKAELGAGDTLPLH
                     GTIFLSINQRSKEELLPEMRLLYDAGYKLIATEGTAAYLNAHGIPCEKVYKVSEGRPN
                     IVDVIKNKQVDLIINTPAGKVPKEDAYTIRQAAVRYHVPIITTIAAAKAAVQGMIFTK
                     KAGHFTVRSLQEYHAEVQQCVKKRF"
     gene            11070..11609
                     /gene="pyrR"
                     /locus_tag="PLJ75_07975"
     CDS             11070..11609
                     /gene="pyrR"
                     /locus_tag="PLJ75_07975"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPD05188.1"
                     /translation="MCEEKILMNKQQIDESIKKISTDIINEFGDMANVTLVGIQTRGV
                     EIAKRIKKLLELHGGNEIKMGTLDITFYRDDLATRGVIPAIKDTIIEFNITKKIVILV
                     DDVIFTGRTTKAALETLMSFGRPQIIRLAVLVDRGNRELPIQPDYCGIKINTKLNDKV
                     KVRLKETDGIEDTVLYCTE"
     gene            11624..12499
                     /locus_tag="PLJ75_07980"
     CDS             11624..12499
                     /locus_tag="PLJ75_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPD05189.1"
                     /translation="MKPTYHCIPCHVNHVYRIISQLSPEADKAHSIMQQLTLMIAKNR
                     YDTPPDCAYVLYKKIKVLTGIDDPFANVKQQNNDIMLSLMPQLQSVLDDDEPIKQALR
                     ISLAGNIIDYGIGDVDYSGYLKKFQDLVHDIDIPDKFYNQFIASVANAKKILFIGDNA
                     GEIVADKLLVEKLNHDNITFVVRGGAVINDATIDDAHYVGLSKKVKIVTTGDRTPGIH
                     LHRSGKDFLQELLHADMIIAKGQGNFETMIDASLQDFVKHGATLFFLFKIKCVPVAEY
                     MGKKVEDNAFVIREL"
     gene            complement(12543..13805)
                     /locus_tag="PLJ75_07985"
     CDS             complement(12543..13805)
                     /locus_tag="PLJ75_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015954926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPD05190.1"
                     /translation="MTHHNKLFGLSPEAGRWIYVITGLIICLCLGSVYAYSIFLNPII
                     NEFSTESYKLSASQGNLPFMVFLAFFSILMFFAGKIMDKLGPKKLTIYGGLVVGLGWA
                     LSYYAVEAKSLNLLILTYGVIAGSGVGVVYGCPLNIVGKWFPDKKGLATGLVLAGFGG
                     SALITGKIANVLIQQSSLSLTFLYFGIGFAILLVLLAFPFKSPEAGWKPQGWTPSASA
                     ASTVDFTSSEMVKTSTFWGLLLAYLIGCLAGLMAIGISKPVGSEIIKIDGETATTLVG
                     IFAIFNALGRPLFGWLTDKISPKGASILNLLIIIAVSIMMLVAKEGDVTMYIIAFCGF
                     WLILGGWLAIAPTTTLTFFGVKNYSRNYGIVFFAYGLGAILGGIISGQAKDAFGSYQV
                     AFWPTLILGVVGLMIVMATLKPPVKKAA"
     gene            complement(13920..14921)
                     /locus_tag="PLJ75_07990"
     CDS             complement(13920..14921)
                     /locus_tag="PLJ75_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HPD05191.1"
                     /translation="MKKGLLFLCGCIAIIATMMSFEMFGKTDTQITVEEFNQPINAPE
                     FYKGIYLNSQSGRDVNLLKNFITRAKDAGFNAVVIDVHNESSNKFLIPKENIDYVIAQ
                     GFYPIARIVMFPDGLKVYPIPEELIKGRLELAKKACEIGFREIQFDYIRFNDYGILKH
                     VPLEERYKTVEGFLARAKSELLPYKVKVACDVFGRIPLNTNDSIGQRMEGLDRVADVI
                     CPMAYPSHYTWSKKMMSDPYYTVLITGKRAKERTKNAEIVMFIQAFKIKVSMSGLSFQ
                     EYMTKQIQAVHDSGVKGFIFWNASQQYDEPIAATRQFYSNFKSQNISKSENPKMETM"
     gene            15221..15655
                     /locus_tag="PLJ75_07995"
     CDS             15221..15655
                     /locus_tag="PLJ75_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPD05192.1"
                     /translation="MRLWSLHPEYLDPKGLVAVWREGLLAKKVLAGKTKGYTNHPQLL
                     RFKNYNNPIKAIDGYLFWIYKEAQKRGYHFTAGKFNEELLIAIIPVTRGQLEYEFDHL
                     LKKVYSRNLNFYERLLDGKNKIICHPVFYCIEGDMEQWEIKR"
     gene            15637..>15922
                     /locus_tag="PLJ75_08000"
     CDS             15637..>15922
                     /locus_tag="PLJ75_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05193.1"
                     /translation="MGNKKIKSAVITNNLFRSLKVYNFRLFFMGQFISLVGTWVQQVA
                     SSWLIYQLTSSSIWLGLSAFAAQIPIFVMTPLSGVVADRFNRHAIFMITQT"
BASE COUNT         4868 a         2829 c         3370 g         4855 t
ORIGIN      
        1 ctgatgaaga cttgcgctgt ggagtacttt ttgctcacgg tgatcatttt acgggcggtc
       61 ttgatctggt acagtgggtt gaaccgttca gcaaaggtga atatccagcg ctgccagccg
      121 gtagcaaaga tccctttggt atagatgaag acaacagggt gtcaaaacca atagtgtctg
      181 ccctgcaggg tatatgcttt acggttggct gggagctaat gcttgctacc gatgtgcgtg
      241 ttgctgccag caatgtacga ttggcgcaga ttgaggtgaa acggggcatt tatcctgttg
      301 gtggtgcaac ggtgaggctt tttgaagaga taggctgggg caatgctatg cggtatttgc
      361 taactggtga tgagatgaat gcacaggaag cctaccgttt aggattggta caggaagttg
      421 ttgaacctgg caagcagctg gagcgagcta ttgaaattgc tactgttata gcaaaacagg
      481 ctccattagg ggtcagagcc attttaaaat cagctcgttt aagcagagtt gaaggcgcaa
      541 aggcggcttt agaacggtta ctgcctgatt tgctgcctat catgaaaagc gaagatgcgc
      601 aggaaggggt cagagccttt attgaaaggc gtgaagctgc ttttaaaggc aaataaacag
      661 taaatagtta tatgatgggg taatcgggtg ctggaggttg ccccattaat gctttaataa
      721 tgtaataggc tatggtaatg gtatacccca gcttcaagcc ggtacttatt aaaaagccaa
      781 taaatgtaag caacgctgct tttaatgctg tcagtgcatt attgtttgca accagttcgc
      841 ctataaatgc accaataaat ggaccaataa ttataccaat aataggtagt ataatacttc
      901 caactattaa ccctatcatt gaaccaataa caccctgttt ggaaccacta aacttattga
      961 caatccatga ggggagtaca taatcaaaga ccgtcaggat agcggcgata attcccgtaa
     1021 taataaaaaa tgtaaaacta aaagaggcaa aaccgctcca atgaagaagg ataactgcaa
     1081 aaaaacttaa aggaggaccg ggcagtatag gaagtaccga acccagaata cctcctaatg
     1141 caaaaagcaa tgctgtaatg aataatatta tttccataat aagtaatata ccttcattgt
     1201 tctttgataa aatacagaag ggcacatgcc ccatgtgccc tgaacttaca gtgtataatt
     1261 tttaccactc gtcgctttgc ttatcaatat atttttcgcg gacaattgct gtaatatcat
     1321 ctatgtaaaa gtaattccat gcgggaagcc gttccaatgt gctgggatta tacagtattt
     1381 taacaatctc tatattccgc ttctgggcta gatatttatc ttcctgagct atctcgtcgg
     1441 taattggaac ggaaagcttt cgccatccta aaaagttaag cttacccatt accagccgtt
     1501 taatattccc attggcatct ttaaccatga tagatagttc tcctgaaaaa tttttcccat
     1561 acacccacat gtttatatat ttgcaatgct tatcgatgat aaggggtttt gccggaataa
     1621 tggtcaatac cgaaccgctt ttggcaaaaa ccttaacacc cagataccgt tttgaatttt
     1681 gtatcggcgc aggataatcg gtacgcattg aaacgcctgc tttttcatct tttacaaccc
     1741 tgaggctaat attcttatct gtatattcta cagtttcaaa atcctcaagt actatttctt
     1801 tatatatctg gtcgctgagt tgcttttcct gttctaaaac tttctgcgct tcttttgctt
     1861 tttcatcctg tgcatagagc atccctatag aacaacatac aatgaaagct atcaatccat
     1921 atgtaactcg cttcatatta ataaaaacct ccttgaaaaa tttataaata cctgtcacct
     1981 ttgccggttg taacacatac agtagcttca ttatagacac tactgtcatg gaatgtcaat
     2041 agaaaatata attatatcaa cctaaaagta taaattttat tgtattaata tctctgcaaa
     2101 tactccaaaa tggtcggatg gatgatatcc gtcatggagt gtagtgcagc atactgttga
     2161 actatggatc atggtacgtg ggattacatt attagtcaga atatagtcaa ttgtttttgg
     2221 ctgtagatca ttgatttttg tgcaatgagc atagagcgaa tcaaactttg tcctggtgtc
     2281 agttacataa tactttttga tattggcatt ctccatagga ttccatgtgt acgggctggt
     2341 attgtttgaa ggcaaaaatg tatcgtgata gccctgtgaa agaaaatagc gcatttcggg
     2401 tgaatcaatc gtagtgttaa aatcacccat gacaatacag ggcgcatcag cggggacaac
     2461 cttttttaaa aattcagcca tagctattga ttcattgtta cgccatttat tgtcagcttc
     2521 aagctgttga atagcctgtg tatattcgtc atccgaataa ccatatgttt cttttaactc
     2581 tttcagcagt atcctgtttt gcggtgtgtc aggtggcgag gcatgccagt gagtaacagc
     2641 tacaaaaaca ggtttttcat tgataataac tctgccctcc agaacctgtg ttgcatcctg
     2701 cgtatggaat gaaaagcagt ttgttatgat gccaccaccc gaaagctttt tatgaccaac
     2761 ctgttgcaac tgcaattgtt ttcgtgccag caatccgtcg ccttctttta aattggtggg
     2821 aatacccaat cttccgatct ttagtccggc aacaccaaca tggtagatat aatcatagtt
     2881 aaggtcactg gcaagccgtt tcatataatc gggtaaaaag ttagcctcgt taattgcaat
     2941 gatatctgga ttcagattgg caatctcttt tagtaaggct tgatagcgtt tttcacgaat
     3001 ttgcggtgtt tcgtattccc ccattttaaa tgttccaata tagtccaatc ctgaccacac
     3061 gttgatggtc attactttta ttgcattgga ttgaaatcct tttggcctat ccgatgcctg
     3121 tttacacgat acatcaacag tcataataac ccccatagca aaaaaaagta tgcatgtttt
     3181 ttttaaaatc tttatcatgg cctcctccaa tcaaattatt tttttgtttg tgcgaaagtt
     3241 actgtacaat aagtcaatat ctctccattg acatggcact ggcatcatgg ttgcctggat
     3301 gttttgtgaa tatgatatac ttttcaaagc atatacaatt gcacatatgt atacggtgtc
     3361 aaccatttta cggttgtatc aatccatagg aactatcgcc catgcttttg taacaaaata
     3421 gttgattgta attttaaaag tgataattta gttactatac tttttttatt tttcgttgaa
     3481 ttaatcattg caaccaattg atgctttatc cagtataaca ttacatagca atgcaaagga
     3541 atagcgggcg cgaagaaaat taaatgaaat gcagacaact aaacatagca acaatgcaat
     3601 ccagtaaatg tttgaaaagg tttttgcaaa catgttgtat gatgattgta atgtttatag
     3661 taaaatcttc ggtgttagct aacggatcat ttaactggtt ctggtttatc tatgagcagg
     3721 gtacatacta cgatagtacc tatacggtat accgaccttt gtatgtgaaa aatgtgtacc
     3781 gtgactcctc gttttctgcc tcgcttatgc cgttgatata ctggaactat acaacgccca
     3841 atcaggataa aacaagaggt tttcttggat tttatgtatc catagattac cggtcagcaa
     3901 atcattatga aaatgacacc ggtgtgttcc cctttgtaat gtatggcact tcaaatgaca
     3961 aagaagacca gtattttcat ctatggccgt ttggtgggac ggttaaaagt aaactgggag
     4021 ctgatgcaat aaccacctat gctttcccgg ggtttgtact ttttatactg tatcctacta
     4081 catcgtatta tgtacttgct gtttatctgg tggcttcaat gattccgatt tatgctcatt
     4141 acaccatcag ggattatgat ggacatgcta tcatctggcc attgatacgg tggggtaaaa
     4201 gtccccaacg tgatgatttc cgtatcatgc cgttttatgc tcatagcaaa aaaaaaggat
     4261 actattcaac tgcatcgtat ggaatgctct ataatgttcg tgaagaatat attggcgatg
     4321 ataccatgca tacggtgttt ctggtgccgt tttatgcacg gcggtggttg gattccggca
     4381 aagctgacag ctctacgctg ttatggccat ttttttcatg ggggtataac gataagcatg
     4441 gcaatgttga gataaatttt ccatggccgt tggtgcagat acgggactgt gatgacccct
     4501 atatctataa aagaatcttt ttcccatttt atggaaaata tatagaaaaa cgtaaaacta
     4561 ccttttttat atccccgttt tattttgtgc ttgaaaaaca aaaggatagc ttccattctc
     4621 aatattcgtt ttatgggata ttgttctggc attttcagcg tgattataag tatggacata
     4681 agcggtatgg gaatcactgg cgcttttata aattatggcc attgtttcac tatgagtaca
     4741 atgataaagg cgacagctcg tttgcaatcc tttcaatatt cccattcagg gatgaagagg
     4801 gatatgagcg attgtatcag ccttttttta gtttggttga gtatgctacc cgtgatgatg
     4861 gtagcaagcg ctttggatta ttactgcgaa cgtacttcca gagctggggc gatagttact
     4921 tcaaaatgaa gattccgttc atcatttcat atcacagcaa agataaaaca gtacagcatg
     4981 tatcgctgtt gctatcgttt ttagagtata ccaatgatga atctatcaat gagttgcgta
     5041 ttggatggat acctgtgtat agaaatgtta aggataaaaa acagtatgat gataaaaaga
     5101 aggtgtcgct acggctgcag caaatagagg gtggctataa ttctatggtt gcttatggaa
     5161 gcaccacgag ctattataat gattctacag gttgcatggt aaggttttaa atatatggtg
     5221 aatacatcta ctgtttcgtg gtataatggt gtattgcata atcagtttag tttattttta
     5281 aggtaagcat atggtcatga taaaaaaatt aagggatgtt atagtcaatg ttgtaggtgg
     5341 tgttggcaaa aacaccttac atattgttta tatgatgggg tattccacca tgttattcct
     5401 tgaggttgtc tattattcac gatcagcctt tgacaaacgt aaagagattt taaaacagat
     5461 gtattatgca ggggtaaaaa cattcattgt tgtttcaatt gtagctcttt ttaccggtat
     5521 gatccttgca ctgcagatag ggcttgagct aaaaccctat cagcaacagc cgcttgtagg
     5581 gaatatcatc atcgcaacgc ttacccgtga aatggcacca tttgtaactg ctatcatcct
     5641 gattgcagcg gttggctcaa ccatggcggc tgaggttggg actatggctg tttccgaaga
     5701 gattgatgca ctggagatga tgtcaataag tcctgtgcgg tttctggtta tgccacgggt
     5761 tatatccctg gcactcatgc ttcctattgc aacggtttat acaaatttgc tgggatgtat
     5821 aggcgggggt atcatagcac attttcagtt aaatgtaagc tttgatgtgt attattcaca
     5881 tattcttgaa tcattgcatt ttaaggcaac ctatgtaggg cttttaaaag cgtttatatt
     5941 tggcataatg gtttctacta taagctgtgc taatggatta cgggctacca atggggcatt
     6001 gggagtgggt aaagcaacgc gaaactcggt tgtttcgtcg tttttaatgg tgcttattgt
     6061 tggttactat atcacgggaa ttttttatgg gaaagcagga taatcgatag ttattgttga
     6121 tagtgtacta tgattgaatt taaacatgta acaaagatat taaatggcgt aaaggtgctt
     6181 gataacctca atataactat tgagaaaggt gaaacctttg ttattatcgg cagatcagga
     6241 acagggaaaa cggttactct gaagcatatc gctggtttac ttgagcctga gagcggtgcg
     6301 gtgcttattg atgggaagca gatgaatggc gccagtagaa gacagaagga tagcttgcgt
     6361 gaaaaaatag gatttgtttt tcagtcaggt gctttaatca actggatgac ggttgaagaa
     6421 aatattgcac ttccattggt agagcataag gtattatcgg cagatgagat taaacaaatt
     6481 gttgaagaaa aattggattt attgcaattg acatctgcgc gtgatagaat gcctgctgaa
     6541 atcagcggtg gcatgaagaa gcgtgcaagc ctggcaaggg tattggtgcg caatcctgaa
     6601 ataatactct atgatgaacc aacttcaggt cttgatcctg ttatgtccag cttgatcaat
     6661 acaataataa accagataaa aaaggatcag catattactc aggtagtagt aacgcatgac
     6721 atggaaagtg cgtatgcaat tggagacagg attgccatgc tgtatgatgg gtcaattatt
     6781 gaatgtgatg ttcctgatgc aattcgcact acaaccaatc ccattgttcg ccagtttatt
     6841 tctggttctc tggaaggtcc catcgaggtt ttgtaatgta tattctatgc gatgggaaag
     6901 cttcatttaa agtatcatgt tatttaaaat gttttaacga tgtgctatac tattcaggag
     6961 gtgtgctatg aaacaggaga tggcagttgg tattgttgtc attttagcta tgacagtatt
     7021 aggatatttt accatcatta tgagtggtga gatcatcgat actcatacat attatccggc
     7081 atcgattgtt tttaaagata tagaagggct ggcaaaggat gataaggtac gtatcaatgg
     7141 cgtattatca gggtatgttg ccggtactca gttgcttgac aataaggtca ttgtaacatt
     7201 aaagctgttt aatcatttta ccttatatga aaactatgaa attatgattc gcaatgaaac
     7261 agcattagca ggaaaatatg tcagtataga tccagggacg cctgttgacg aaaatgggaa
     7321 acagtatgca gttgttacca cgcgggaaaa tctttcaggt attgctgtaa gcgatccatt
     7381 tacattgctt tcaaggctta ttgctgacaa caggggtaat gtaaattcgg tactcaaaaa
     7441 tatacgggat ataactgata agattaattc cggcaaaggg acattgggca aaatcatcaa
     7501 tgatgctaca gttcatgatc aagccagtgg cttaataaag gaattgcgtg atactattga
     7561 ggataccaga gaacaggcac ctattaccag ctttttgcgt gctgctttaa ctgcttttta
     7621 acaaaaaaat gctgccctgc cgttattatt gttttttttg gaaaaaattt gtctacggga
     7681 aagctgtgct ctttgctttt ttactcacaa tcatattgtg tgataacaca tagaaaggat
     7741 tcaatataga tgcccaggcg aacggatatt acaaaaattc ttattatagg ttcaggtcca
     7801 attgttattg gtcaggcgtg tgagtttgat tattcaggtt cacaggcatg caaggctctc
     7861 agggaagaag gttatacgat agttcttgtt aattccaatc cagcaaccat tatgaccgat
     7921 ccggagattg ctgacaggac ctatattgaa ccaattactc atgatatagt ggcacagatt
     7981 attgaacgtg aaagacctga tgctctgctg ccaacagttg gcggtcagac tgctttaaat
     8041 atagcagtac agctttatga cagcggtaca ttacaaaaat atggtgttga gctcataggt
     8101 gcaaaaattg aagccataaa aaaagctgaa gacagggacc tatttaaaaa agcaatgctt
     8161 tcaataggtt tacaggttcc ccgttctgta ttggcttcct caatagatga agcactaacg
     8221 gttcttgaat ccatatcgct tcctattatc atacgtcctg cttttacatt gggtggtacc
     8281 ggcggcaata tagtgtataa ccaggaagat tttattgaac tggtgcgcag gggattggat
     8341 gaatcgccta tcagtcaggt tttactggag gagtcagtta ttgggtggaa ggaatttgaa
     8401 ttagaggtaa tgcgtgattt aaacgataat gttgtcattg tgtgctcaat agaaaatttt
     8461 gatcccatgg gtatacatac cggtgattca atcacggtag ctccacaaca aaccttaacc
     8521 gacagggaat atcaggtttt acgtgatatg gcaatagcaa taataaaaga gatcggcgtg
     8581 gaaaccggtg gttctaacat tcaatttgct gtaaacccag acgatggaag gattatggtt
     8641 attgagatga atccacgcgt tagccgcagc tcagcattgg cgtccaaggc taccggtttt
     8701 cctattgcaa agattgcagc aaaattggcc atagggctta cccttgatga aatccgcaac
     8761 gatatcaccc gtgaaacacc ggcgtgtttt gagccaacca tagattatgt tgttaccaaa
     8821 ataccacggt gggcatttga aaaatttgag ggtgctgata caacgttagg gacacagatg
     8881 aaatctgttg gcgaagcaat ggctataggg cgtaccttta aagaatcatt tcaaaaagct
     8941 ttacgctctc tggagattga acggtatggg tttggctcgg acgggaatgt aaagattgat
     9001 gcaatgcttg atggattatc tgaaaataaa aaaaatgaca ttatagaaat gaatttgaga
     9061 attccaaagg aaaatagagt tttttacata aagaaagcac tggaattaca ctggagtgtt
     9121 gataccatat attcactaac atcaatagat ccatggtttt tgcatcagct taaagaatta
     9181 gttgatgccg aacatgattg tattgcacaa ttaaacagtg ggttatcaaa agatattatt
     9241 gtacggatga aacaatgggg atattcggac aggcaactgg catatttagc acataaggat
     9301 gaattgacat tactgtatgc gaaggggatt gattcacaga aggattattc aaaacgattg
     9361 tccgagcttg aagatgatat aaggaactat cgccaaaaaa atggaattgt ccccggctac
     9421 cgacttgttg atacctgtgg cggcgagttt gaagcataca cgccctatta ctattcgtcg
     9481 tatgatgatg aggatgaagc caggtattcg gataaacgaa aaataatgat tctgggtggc
     9541 ggccccaaca ggattgggca gggaatagaa tttgattatt gctgctgtca tgcgtcattt
     9601 gctctgcgtg atgctggcgt tgaatccatc atggtaaact ccaaccctga aacggtttca
     9661 acggattatg atacctcgga caggctatac tttgaaccgc ttacactgga ggatattatc
     9721 catatatatc aaaaagaaaa acctgaaggg gttattattc agtttggtgg gcaaacaccg
     9781 ttgcgattgg cacagcgtct ggaggcgaat ggggtaaaaa ttcttggcac tccacccgat
     9841 gcaattgata gggctgaaga cagagagcgt tttgcacagg ttgtaaaaaa attaaattta
     9901 catcagccgg aaaatggtat agcatttacc cagaagcagg ctcttgaaca ggcaaacagg
     9961 atagggtatc ccgtgctggt gcgtccatca tatgtgcttg gtggaagggc aatggcaatc
    10021 atgtatgatg aaaaaagcct gattgagtat atagtttctg ctgcagaact atcgcccgag
    10081 catcctatcc ttattgataa atttttagat gatgctcttg aaattgatgt tgatgcgctg
    10141 tgtgacggca tggatgtcta tgttgctggc attatggagc atattgaatt ggctggaatt
    10201 cattcgggtg actcggcatg ctcgttgccg ccggtgagcg ttaagcctgt aatgatgcac
    10261 accattatgg caacaacggc tgcgttagca cacgagcttg gtgttattgg attattaaac
    10321 atccaatttg caattaaaaa tgatgtgctc tatgttctgg aggttaaccc ccgtgcttcg
    10381 cgtacggtac catttgtatc taaggcaaca ggcgttccgc ttgctaagat agccgtgcag
    10441 atacttttgg gtaaaaagct caaggatttt ggattaacac aggctaacac tatgccgtat
    10501 tgtagtgtta aagaggcggt attacctttt aataaatttc caggtgtgga tacattgcta
    10561 tcgcccgaga tgaaatcaac cggcgaggtg atgggtatag ctaacaattt tggcgaagca
    10621 ttttataaag ccgagttagg agcgggggat acgttgccgt tgcacgggac aatattttta
    10681 agtatcaacc agcgttcaaa agaagagctg cttccagaaa tgcgtttgct ttatgatgca
    10741 ggctataagc tcattgccac tgaaggtact gctgcctacc ttaatgctca tggtatcccc
    10801 tgtgaaaagg tgtataaggt aagcgaagga cgtccaaata ttgttgatgt tattaagaat
    10861 aaacaagttg acttgattat caataccccg gcgggcaaag tacctaaaga agatgcctat
    10921 accatacggc aggcagcagt acgctatcat gtgccaatca tcacgaccat tgctgctgca
    10981 aaagctgctg tacaggggat gattttcaca aaaaaagctg gacatttcac ggtgaggtcc
    11041 ttacaggaat atcatgcgga ggtgcaacag tgtgtgaaga aaagattttg atgaacaaac
    11101 agcagattga tgagagtata aaaaaaatta gcactgatat tatcaatgaa tttggcgata
    11161 tggcaaacgt aacacttgtt ggcatacaga cacgaggtgt tgaaattgca aagcgtataa
    11221 aaaagcttct ggaattacac ggtggtaatg aaataaaaat ggggacgctg gatataacat
    11281 tttataggga tgatttagct acacggggtg ttatacctgc tataaaagat accatcattg
    11341 agtttaacat taccaaaaaa atagttatac tggtggatga tgttattttt acgggcagaa
    11401 caacaaaagc tgcactggaa accctgatgt catttggcag acctcagatt atacggctgg
    11461 cagtgctggt tgacaggggc aacagggaac tgcccataca gcctgattac tgtggcatta
    11521 agattaatac aaaattgaat gataaggtaa aagtgcgtct taaagaaaca gatggtattg
    11581 aagatactgt attatattgt acggaatgat acagaattaa accatgaaac ccacatacca
    11641 ttgcattccc tgccatgtaa atcacgttta cagaattatt tcgcagctat cacccgaggc
    11701 tgataaagct catagcatta tgcaacagct cacattgatg attgcaaaga atagatatga
    11761 cacaccacct gactgtgcat atgtgctcta taaaaagata aaagtattaa cggggattga
    11821 tgaccctttt gctaacgtta agcaacagaa caatgacata atgctatcat tgatgccaca
    11881 attacaatcg gtgcttgatg atgatgaacc aataaagcag gcgttaagga tatcgctggc
    11941 aggtaatatt atagattatg gcattggtga tgtggattat tcagggtatc tcaaaaaatt
    12001 tcaagattta gtccatgata tcgatatacc ggataaattt tataatcagt ttattgcttc
    12061 ggttgccaat gcaaaaaaga tattgtttat tggagataat gcaggtgaga tagtagctga
    12121 taaactgctg gttgaaaagc tgaaccatga taacattacc tttgtcgtca ggggaggcgc
    12181 tgtgataaat gatgcaacaa ttgacgatgc tcactatgtt ggattaagca aaaaagtaaa
    12241 aatagtgacc accggtgata ggactccggg tatacatttg catcgcagcg gtaaagactt
    12301 tttgcaggaa ttactacatg ctgatatgat aatagccaaa ggtcagggaa actttgaaac
    12361 catgatcgat gcttctttac aggattttgt aaaacatggt gcaacattat tctttttgtt
    12421 taaaataaaa tgtgttcctg tagcagaata catggggaaa aaggttgagg ataatgcttt
    12481 tgtaatacga gaattgtaaa aggggcacat attgttgtgc cccttttaca ctattcatta
    12541 tgttatgcag cttttttaac aggtggtttg agtgtagcca taacaatcat caaacctacc
    12601 acacctaata tcagagtagg ccagaaagct acctggtatg atccgaatgc atcttttgcc
    12661 tgccctgaaa tgattccacc aagaatagca cctaacccat aagcaaagaa tactataccg
    12721 taattgcgtg agtaattctt taccccgaaa aaggtaaggg tagtagttgg agcaattgca
    12781 agccatccac ccaggatgag ccagaatccg cagaatgcaa taatatacat ggttacatca
    12841 ccttcttttg caacaagcat catgatggac actgcaatga tgatcagtag attaaggatg
    12901 gatgcacctt ttggtgatat tttatcggta agccatccaa aaagaggacg tcctaaagca
    12961 ttgaatattg caaaaatacc aaccaatgta gtagcggtct ctccatcaat ttttataatt
    13021 tcgctaccaa caggttttga aattcctatt gccattaaac ctgctaaaca gccaataagg
    13081 taggctaata ataatcccca gaatgttgag gtctttacca tttcgctgga agtaaaatca
    13141 acggtactgg ctgcagatgc tgaaggggtc catccctgtg gcttccatcc agcttccgga
    13201 cttttaaatg ggaatgccag aagaaccaga agaatggcaa atccaatacc aaaatagagg
    13261 aatgttaatg ataagctact ttgttgaata agtacgttgg caatttttcc tgtaattaac
    13321 gctgatccgc caaagccagc aagtaccaaa ccggttgcaa gtcccttttt atcggggaac
    13381 catttcccta caatatttaa tggacagcca tatacaacac ccacaccgct accagcaata
    13441 acaccgtatg taagtattaa aagattaagg ctttttgctt caacagcata atacgatagt
    13501 gcccagccta atccaaccac caatcctcca tagatggtta atttttttgg acctaactta
    13561 tccattatct ttccagcaaa gaacatgagt atggaaaaga aagctaaaaa gaccataaat
    13621 gggagattgc cctgtgaagc actgagctta tagctctcag tactaaattc attaattatt
    13681 ggatttaaga atatgctata tgcatataca cttcccagac ataagcatat aattaaacca
    13741 gtaataacat atatccatcg tcctgcttca ggcgatagac caaaaagttt gttgtgatgg
    13801 gtcatataaa ccaatcctcc tgtatgtttt ttaataaatt ataatttaaa tcacaaaaga
    13861 caattgctga aagcaaaaat gattagcagt ataatagtca agaatatttt tacttattgt
    13921 cacattgttt ccattttggg attttctgat tttgaaatat tctgagattt aaaatttgaa
    13981 tagaattgtc tggttgcagc aattggttca tcgtactgct gtgaggcatt ccagaagata
    14041 aaacctttta cccctgaatc atgtacagcc tgtatttgtt tggtcatgta ttcctggaat
    14101 gataatcctg acattgatac ctttatctta aatgcctgga taaacataac tatttcggca
    14161 tttttggtgc gttcttttgc ccttttacca gtgattaata ctgtatagta aggatcggac
    14221 atcatctttt tgctccatgt atagtgggat ggataagcca tggggcatat cacatcggca
    14281 accctgtcaa gtccttccat acgctgtcca attgaatcat tggtattaag gggaatcctt
    14341 ccaaagacat cgcatgcaac ttttaccttg tatggcaata attcggattt tgcacgtgct
    14401 aaaaatcctt caacggtttt ataacgctct tccagtggaa cgtgttttaa aataccatag
    14461 tcattgaaac ggatataatc aaattgtatc tctctaaatc caatttcaca ggctttcttt
    14521 gccaattcta atctgccttt aataagttct tcagggatgg gatatacttt taatccatcc
    14581 gggaacataa cgatacgcgc aattgggtaa aaaccctgag cgataacata atcaatattt
    14641 tctttgggga tcagaaattt gttgctggat tcattatgga cgtcaataac cacggcatta
    14701 aagccggcat cttttgccct ggtaataaaa ttttttagta aatttacatc tcgtcctgat
    14761 tgcgaattta agtaaattcc tttataaaat tcaggtgcat taatgggctg gttaaattct
    14821 tccacggtga tttgcgtatc agttttacca aacatctcaa agctcatcat ggtggctata
    14881 atagctatac acccacaaag aaacagtaat ccctttttca tgtttttccc ttttctaaaa
    14941 gattttgatt aataaaaatc gtgtgtacat gatggtacta tgtaattatt tgtcgactaa
    15001 aatatgatat cattgtacac aaaaatgaag cacaaaacat atgcctggaa tccttcttga
    15061 ataatacatg gtagatgaat gctttcattt tattctttaa tacagtcttt ataaagcaaa
    15121 tgctggttgt gctatcttga aattgttatt tacattttta tagtaacatg taatacggct
    15181 taaaaaatgt atgggcgata gttattgata atgaaatggt atgcggttat ggtcattaca
    15241 tcctgagtat cttgacccta aaggcctggt tgcagtatgg agagaaggat tgttggcaaa
    15301 aaaggtattg gctggtaaaa caaaagggta taccaatcat ccgcaattgt taaggtttaa
    15361 aaattataac aatccaatca aagctattga tggctatctt ttctggattt ataaagaagc
    15421 acaaaagcgc ggttatcatt tcactgctgg gaagtttaac gaagaactac taatagcaat
    15481 aatccctgtt acaagaggtc aacttgaata tgagtttgat catttattaa aaaaagtata
    15541 tagcagaaat cttaactttt atgaaaggtt gctggatggt aaaaacaaaa ttatatgtca
    15601 tcctgtattt tattgcatag aaggtgatat ggagcaatgg gaaataaaaa gataaaatct
    15661 gctgtcataa ccaacaatct attccgctct cttaaagtat ataattttag acttttcttt
    15721 atgggccagt ttatatctct tgttggcaca tgggtgcaac aggtcgcatc aagctggctt
    15781 atatatcagc ttacctcatc atcgatatgg ttggggttat ctgcatttgc agcacagata
    15841 cctatctttg tcatgacacc actttctgga gttgttgccg accgtttcaa caggcatgct
    15901 atctttatga ttacccaaac ga
//
LOCUS       DAOOJH010000053        15421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3697_length_15421_cov_10.133802, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000053 DAOOJH010000000
VERSION     DAOOJH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15421
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_15421_cov_10.133802"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..566)
                     /locus_tag="PLJ75_08005"
     CDS             complement(120..566)
                     /locus_tag="PLJ75_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD05194.1"
                     /translation="MESRNVLLIFNQNIMYKPIIYRIAKDFNVIFNVLEAKILPKQEG
                     RLILQLSGEQEDIDTCINYLINEEVIVDILADKIQRDEDRCVHCGACTAVCRTDALVI
                     NRETMMVEFYPERCVACGLCKLACPVKAMSGISIDVDSEELAIKVI"
     gene            complement(578..1303)
                     /locus_tag="PLJ75_08010"
     CDS             complement(578..1303)
                     /locus_tag="PLJ75_08010"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPD05195.1"
                     /translation="MNNTLAYRALIQDTRFTTFEVCVETTSLFVCAHNNLGDRTLQLV
                     HWLRNDIKDYIAKHDNFLHALSPLEFDPSAPEVVRRMYSASAKASVGPMAAVAGTIAQ
                     MIGEELTAYSPEIIIENGGDIWAKVQEPAVIKVFAGRSSFSDDCGIVIQPHDTPLGIC
                     SSSGRFGHSFSFGKADCVTILASSASLADAVATATCNRVQTEEDCVHALEYACTISGV
                     TGALVIYWDTMAMKGCIELTQTS"
     gene            complement(1337..2527)
                     /locus_tag="PLJ75_08015"
     CDS             complement(1337..2527)
                     /locus_tag="PLJ75_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPD05196.1"
                     /translation="MSDIVIKKSYEAINNKIKKGEAVVVTADEMTSIVKSHGYKSAFK
                     KVDVVTTGTFGTMCSSGAFINFGHTRPKIKASKVWLNDVEAYAGLAAVDIYIGATQVQ
                     EDDPLNKVHPGRFKYGGGHVIEDLIAGKPVKLKAKSYGTDCYPLREYEATVTINDLRD
                     AFLYNPRNAYQNYNCAVNQSKKTIYTYMGILRPNMGNATYSSAGQLSPLLNDPYYWTI
                     GVGTKIFLAGSVGAVTWRGTQHSPNEPRSENGVVTSGAGTIAVTGDMKHMTTQFIKGA
                     SITGYGCSLMVGIGIPIPILNEDLAFFTGVSDDAIYCQVVDYGYDYPNAISRSIAQVS
                     YAQLKSGFVEIAGKRIPTAPLSSYPMAKKIADILKDWIVQRQFTVGEPQILLPTVPYQ
                     KHHE"
     gene            2826..3446
                     /locus_tag="PLJ75_08020"
     CDS             2826..3446
                     /locus_tag="PLJ75_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05197.1"
                     /translation="MPRLKPHARAEKEELRKTQITQSAYEVISAKGYNNFTLDDIAKN
                     AGLSKGGVLHYFKSKEDILLYLLEQIYIIIKKNINKRSSKYRTPERRLRAIVIAFIIT
                     AKRHPAFYTVLVDFWAQIPIHERVKYINTQIYKQLCDEVIKVIEDGIASGVFKQVHAD
                     NTAHAIVAMVMNVAIQWTFNHELYNIDHMAKTCMKMIMAYLEEKTI"
     gene            complement(3439..4092)
                     /locus_tag="PLJ75_08025"
     CDS             complement(3439..4092)
                     /locus_tag="PLJ75_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05198.1"
                     /translation="MAQATSQDIVHEILSAINRNQEIVFVSYSILDETEKNIQFALNK
                     ILERHNKSDLYTPVYSSLKELVANAIKANAKYILIEEGEIKDPNDIMEVVNKVRNILN
                     EESLLLYGIKAKQYRLSTRIYLKPQTTRLTIEVINNLPVPQKELERMRERIERSSYYD
                     SIADFYLENPDPTAEGMGLGLSMVVVLLKSVGIPYTNFTLYTDFKSKTVARIIIPLI"
     gene            4236..5099
                     /locus_tag="PLJ75_08030"
     CDS             4236..5099
                     /locus_tag="PLJ75_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPD05199.1"
                     /translation="MFNMLFPSVSSITVITLLSLVCGILLSLAKVLLKVEKDPRIEKI
                     VESLPGANCGACGFPGCVGYATHIIEKGASINLCPVGGPESAQKISQIMGIEAEIVAK
                     RIARVHCQGGINETKIRFMYDGPHSCTAAQLVMGGFKVCEYGCLGLGDCVRACLFDAI
                     HLDKRGLPLVDAEKCTGCGNCVEACPRNIISLLGENIDVYMMCRNKQKAPVMKLGCNV
                     GCTACRRCEKVCREEVFKDNPDVDTAIKVVDFLAVIDYELCINCGRCAEVCPQNVIDF
                     KKVMVIKRETN"
     gene            5086..6102
                     /gene="fliG"
                     /locus_tag="PLJ75_08035"
     CDS             5086..6102
                     /gene="fliG"
                     /locus_tag="PLJ75_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HPD05200.1"
                     /translation="MKPIDQMSGAERAAALLVALGPEVASEIMKFLDDESIQKLASEI
                     AKIEVLSPQEKEDLIGQFLLELKKTRGITFGGQNVAKEILYAAFGQDKAQDILKRLTH
                     KDLEKGFGFLNDIDPELIVTFLHNEHPQTIAVTLAYLPAAKAAEVIKLLPTDIAKEVT
                     LRMAKMERTSPDAVLEISRVLRKKYDEYRYQGQSSSKAGGLNALIDIMSHLSGDQEKQ
                     LIDYFETTMPQIADEIRARIFTFENVITLTNTEMRILIDEINNDYMIARALKGAGDEI
                     RFKFFRNMSKNRATDIMNEMEAMGPVRLSEIQSARDNIIRVMRILHDNGVISLRKEKE
                     KYIE"
     gene            6095..6928
                     /gene="rsmA"
                     /locus_tag="PLJ75_08040"
     CDS             6095..6928
                     /gene="rsmA"
                     /locus_tag="PLJ75_08040"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPD05201.1"
                     /translation="MNKKEILSWLQQYGLAPNKMYGQHFLFHEKYIEAIVTAISPCSE
                     DVILEIGPGLGVLTQRLSEHAKKVIAVEIDAGFVEVLQSRFANNSRVTIVHNDFLKTN
                     PYDECTVLTGNLPYNIASQIIFAAAHNFTAPHCFFLLQKEMAQRLIAKPKSAMYSAFT
                     VGIGLYFTVQTLFDIPPQAFYPEPKVYSTFVKLTRKNSVMLHTDEKAVFEQLVATAFW
                     ARRKMLKTALKRSPYTTYREAFISQAFGQLGFDEKVRGEELTLDEFVALAKYCAHYGN
                     K"
     gene            6950..9382
                     /locus_tag="PLJ75_08045"
     CDS             6950..9382
                     /locus_tag="PLJ75_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPD05202.1"
                     /translation="MIPEETIYNLLTTVSKPARYAGGEWNAVVKPHARVKVGLCFPDL
                     YEVGMSNNGIQILYHAINGMQHAAAERVFAVEHDFENQLRQSNIPLYTLETYTPLCAL
                     DMLGFNAACELLYTNILQVIDLGTIPLYAKNRGDSYPLIIIGGEAMSNPAPMLPFADA
                     IFVGDGEEAIIRIAQVLIDKKDKHLLKDEVLTKIAAIEGVLVSRDVEYGYTGNAITAI
                     KAPAVYKRVARRFHDYEVTRPVVPSIRSVQDRVSVQLDRGCKNLCKFCHAGYWELPYR
                     KVEVSRVAQAIVTTLQSTGFNDVSLFSLSVGDYTNIVSLLNIIVPQLIEQGVSISLPS
                     LRVDMQSIAIIESISDLKKTALTFAVESASDALRYRAYKRLTIDEIMNIITELSRRGW
                     QRIKLYFMIGLPGCDDTDEAAAIIAFMKQACSIDKRMRYNVTISPFVPKAHTPFQYEK
                     QYDEHYCLEVVNTIKRNVPKNVTIKNHDVYASLIEGVLARGDSMLADAIAGAYNAGAR
                     LDLWGEHFDFALWDNALQTSCGNWRRYLNRRSDQELLPWSMIQTRYERLIEKKKDCTW
                     NGRGLPHGFKNEILNREAFAQAAQQFTMRYALAATLRMHYAKKGKARFIGHIDSMEIV
                     KRALRMAHIPVSYTQGYNKHERLITGPALPLGYESYSEFVDVQLYESCSAMSLQKLQV
                     SFPDGFEVVNLYHHDASTLPLKRIVFAEYLLQFTDNYRFTLFINRLESKNDIVKTTKK
                     GLMNLRFDDAIAGVIVADSSIRLVMALGEHAIRPDTLVADWCGVHNPLTIAAITKVAS
                     YYLHNDELKKFH"
     gene            9398..10162
                     /locus_tag="PLJ75_08050"
     CDS             9398..10162
                     /locus_tag="PLJ75_08050"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD05203.1"
                     /translation="MGSLSYNLDGKVAIVTGGTRGIGLAIAQELLKQHAKVVICARKQ
                     EGLDRVKEMLKAGDDLLTCVAHIAKEDDVNALVDAAVQKYSTVDILINNVGMNLMTGS
                     LVDVELSAWQKIIDSNLTGTFLVSKKVGAIMRQQKRGKIVSISSVAGRLASPGMNVYG
                     IAKAGVEMMTKNLAVELAPFNVQVNAVAPGMVRTDFSKPFWSNEAIHDMVVKNIPMGR
                     IAEIDEVVHPVLFLASDGARYITGQTIVIDGGASIL"
     gene            complement(10163..11827)
                     /locus_tag="PLJ75_08055"
     CDS             complement(10163..11827)
                     /locus_tag="PLJ75_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HPD05204.1"
                     /translation="MNLTILLLGVNARYIHSSLPLYYLKQILQPLNYTVYLHETTINQ
                     PVDDTRNAIVTINPDVLCLSVYIWNATYIKKLLPMVHTALPNCIIILGGPEVSYTADE
                     WLKHFPFITTIVQGAGERSFEYAAQKQFLLDTKTVSLPNYHLNEIPFPYSESDINNVA
                     SRFIYYESSRGCPYACSYCISSREDQSIQFRDLSKVYAELDIFLQHRVRMVKFVDRTF
                     NCNPTHARSIFYYLLSHNNNHTHFHFEIHPDLLFDEDFTLLQQVPKNYFQFEIGIQSI
                     HPKTLSSINRKMNWEQAKENIAKLMELNTIHIHLDMICGLPYEDIEAIKESFNEIIEL
                     KPHYFQMGFLKILPGTKIASQKNEFGIEHDAEAPYTVIKNNWIPGHMMQALHCIERVV
                     DRFYNHHFKTTWMMLCHFITSGDSFKSFEMIAQFFYKKNFVLFAKDWEAIATMILTFI
                     KEEYPPVEELAIDCLRWDWFIQSNSKWVPACVRSKTNPNIVKEIIINNHKIAKDELLL
                     DGKRIPVRTIQHAQIFIAESKTFQQWHMAGHHYAIKLHRDTILYSA"
     gene            complement(11896..13215)
                     /locus_tag="PLJ75_08060"
     CDS             complement(11896..13215)
                     /locus_tag="PLJ75_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HPD05205.1"
                     /translation="MTLKNTEIQKAEGKLGVVIPGLGAVSTTFIAGVMLIRKGLKSPK
                     GSVSMMGKLKIGKGDDAHYVPIKDFVPLANLQDIEFAGWDIFEDNAYEAAINAGVLQK
                     EDLEPIKDELSKVKPWKAVFFNEYVKKLHGTWTKKAPSHWELAQMVKEDILNFKKEKN
                     LSRVVMVWCASTEIYQEMKPGVHDTIENFEKALKENNKYIAPSMIYAYAALDLGIPFA
                     NGAPNLCNEIPALRQLAEKNKVPMGGRDFKTGQTLMKTIIAPGLKARMIGVDGWYSTN
                     ILGNRDGEVLDDPESFKTKEVSKLSVLDNIFQPDEYPELYDGYYHVVRINYYPPRRDN
                     KESWDNIDIRGWLDYPMQIKVNFLCRDSILAAPVVLDLVLFFDLAQRATMYGIQEWLS
                     FYWKSPMFEPGEEPIHDLFVQLMKLKNTLRWLMDVPPVTYLGSESYD"
     gene            complement(13507..14955)
                     /locus_tag="PLJ75_08065"
     CDS             complement(13507..14955)
                     /locus_tag="PLJ75_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HPD05206.1"
                     /translation="MNAPLLIAKGEKDLCIIPRMANRHGLIAGATGTGKTVTLQVIAE
                     NFSLAGIPVFMADVKGDLSGISKPGKPDDKLAQRLKKLKIDNHTFQGFPVVFWDLFGK
                     SGHPIRTTVTDMGPLLLSRILNLNETQTGTLNVIFKIADDNGLPILDIKDLRSMIQFA
                     GDNASKFNTEYGRVSTASIGAIQRSILELQNQGAELFFGEPALNIEDFIQTDETGRGY
                     VNILSAESLINTPKLYATFLLWLLSELFEILPEVGDRDKPKIAFFFDEAHLLFNDAPR
                     ALLEKIEQVVRLIRSKGVGVYFITQNPADIPQTVLGQLGNRIQHALRAFTPNDQKAVK
                     AIAQTFRENSKLDIEKAIIELEVGEALVSFLDEDGKPEIVERAFIVPPKGQIGPITPQ
                     ERNEIIDASPVKGIYDKTIDRESAYEMLKTRAQKAVEATHEKEDIPKQRAGRKSDTVV
                     EAMMKSAARSIGSSVGRQIVRGILGSLLGGKR"
BASE COUNT         4546 a         2976 c         3149 g         4750 t
ORIGIN      
        1 tggcagtcaa cccttctttt tcaaaaaggg ttgcacttgt gacttgaata caagttttat
       61 gataaatgaa aatattatta cgatgaactt actatgtgtt ttcacagtaa ctttgaacgt
      121 tatattacct ttatagctaa ctcttccgaa tcaacatcaa ttgaaatgcc gctcatagcc
      181 tttacagggc aggctaattt acacagtccg catgcaacac agcgctcagg ataaaattcc
      241 accatcattg tttcacggtt tatcaccagc gcatcggttc ggcatactgc tgtacaggca
      301 ccgcagtgta cacatctatc ctcatcacgc tgaatcttat cggctaaaat atcaacaatt
      361 acttcttcat ttatcagata attgatacag gtatctatat cttcttgttc gccggaaagc
      421 tgcaatataa gcctgccttc ctgcttgggc aatatttttg cctcaagcac attaaatatt
      481 acattaaaat ctttagcaat gcgatagata atgggtttat acattatatt ctggttaaat
      541 attaaaagca catttcgtga ttccatgata acctcgttta tgacgtttga gtaagttcta
      601 tgcagccttt catagccatt gtatcccagt atatcaccag agctcccgta acgccagata
      661 ttgtacaggc atactccagg gcatggacac aatcctcttc tgtttgtact ctgttacagg
      721 ttgctgttgc aaccgcatca gccagtgacg ccgacgatgc aagtattgtt acacaatcgg
      781 ctttgccaaa actaaatgag tgcccaaaac gccctgatga cgagcaaata cccagtggcg
      841 tgtcgtgcgg ttgaataaca ataccacaat catctgaaaa tgatgacctc cccgcaaaaa
      901 ccttaatgac agcaggctcc tgtacctttg cccagatatc accaccgttt tcaataatta
      961 tctccgggct ataggcagtc agctcttcgc caatcatctg ggcgatagtt ccggcaacgg
     1021 ctgccattgg accaacggaa gcctttgctg aagcactata catcctccgt actacctccg
     1081 gtgcagatgg atcaaattca agaggtgata gcgcatgcag gaagttatca tgttttgcaa
     1141 tgtagtcttt aatgtcgttg cgcaaccaat gaaccaattg cagggttcta tcgccaagat
     1201 tgttatgagc acatacaaac aggctggtgg tttcgacaca aacttcaaat gttgtaaagc
     1261 gtgtatcctg aatcagtgct ctgtatgcca atgtgttgtt cataactgta tgctcatggt
     1321 gctgtaacag tttagtttac tcatgatgtt tttggtacgg aacggttggc aacagtatct
     1381 gcggctcgcc aaccgtaaat tgcctctgca caatccagtc ttttaaaatg tcggctattt
     1441 tttttgccat tggatagctt gaaagcggag ctgttgggat acgcttgccg gcaatttcaa
     1501 caaatcctga ttttaactgt gcatagctta cctgggcaat agatctgctg atggcatttg
     1561 gatagtcata accgtaatca accacctggc agtaaatagc atcatccgaa acgccggtaa
     1621 aaaatgctaa atcctcattt aaaatgggta tgggtatgcc aatcccaacc atgagactgc
     1681 atccgtaacc ggtaatggat gcacctttta taaactgcgt tgtcatgtgc ttcatatcac
     1741 ctgtgacagc tattgtgccg gcgcctgatg taaccacgcc gttttcgctt cgcggttcgt
     1801 tggggctatg ctgtgtgcca cgccaggtaa ctgcacccac actgccagcc agaaatatct
     1861 ttgtgccgac gccaatggtc caatagtacg gatcatttaa cagcggacta agctgtccgg
     1921 cacttgaata ggttgcattc cccatattgg gacgcagtat gcccatatag gtgtagatag
     1981 tctttttgga ttgattaact gcgcagttat agttttgata ggcattgcgc ggattgtata
     2041 aaaaagcatc gcgcagatca ttgatggtta ccgttgcttc atattcacgg aggggatagc
     2101 agtcggttcc gtaacttttt gcttttaatt ttacaggttt gccggctatt aaatcttcaa
     2161 taacatgccc accaccgtat ttaaatctgc cgggatgaac cttgtttaaa ggatcatcct
     2221 cctgtacctg tgttgctcct atgtagatat caacggcagc aagccctgcg tatgcttcca
     2281 catcgttaag ccacaccttt gatgccttta tcttgggacg ggtatgccca aagttaataa
     2341 atgcaccgct ggaacacatt gtgccaaacg ttccggtggt aacaacgtca acctttttaa
     2401 atgctgattt atacccatgg gattttacaa tgctggtcat ttcatcggca gttacaacaa
     2461 cagcctcacc tttttttatc ttattgttta ttgcctcata actttttttt atgacaatat
     2521 cgctcattgc atggttatcc tttttgtaat attggggtga atgaacatta tgcttacaat
     2581 actatgagca tgcaagtaaa aattattttt ttaaatttta tagcaaaaat cccatgggta
     2641 tggcaaatgg caaagaagtc ctgagtcggt tacaaatatc tccgcatttt ttataaattt
     2701 tgttaaaaaa actttacaaa taagtttaaa atagccataa ttgtataaac aatatatttg
     2761 ttacattgta cagattgtta tgtaccatga aaacatatgg aatataaaac aaaaaggaaa
     2821 attcaatgcc acgattaaaa ccccatgctc gtgctgaaaa agaggaatta cgcaaaacac
     2881 agattaccca gtctgcttat gaggttatat ctgctaaagg gtataataat tttaccctgg
     2941 atgatattgc aaaaaatgca ggactttcaa agggaggtgt actgcactat tttaaatcta
     3001 aagaggatat actgctgtat cttctggagc agatttacat cattatcaag aaaaatatta
     3061 ataaacgctc ttcaaagtat cgtaccccgg aacgcaggtt acgagcaatt gttattgctt
     3121 ttattataac tgctaaacgt caccctgcgt tttatacagt gctggttgac ttctgggcgc
     3181 agatacccat acatgagcgg gttaaatata ttaataccca gatatataag caactttgtg
     3241 atgaagtaat caaggtcatt gaggatggta tcgcatcagg tgtatttaaa caggtacatg
     3301 ctgataatac agctcatgcc attgtggcta tggttatgaa tgttgccata cagtggacgt
     3361 ttaatcatga actttacaat atcgatcata tggcaaagac atgtatgaaa atgatcatgg
     3421 cttatcttga agaaaaaact atataagggg tattattata cgggcaactg tctttgattt
     3481 aaaatcagta tataatgtaa aattggtgta tggtatccca acgcttttta atagtactac
     3541 aaccatggat agcccaagcc ccatgccttc tgcggtaggg tcaggatttt caagataaaa
     3601 atcggctatg ctgtcatagt aagatgaccg ttcaattctt tcacgcatgc gttccagctc
     3661 tttttgaggt accggtaagt tgttgataac ctcaatggtc aatcgtgttg tttgcggctt
     3721 tagataaatt cgtgttgata acctgtattg ttttgcctta attccataga gaagcaacga
     3781 ttcttcatta agtatatttc taaccttatt aaccacttcc ataatatcat tgggatcttt
     3841 aatctcccct tcttctataa gtatatattt tgcattggct ttaattgcat ttgcaactaa
     3901 ctcttttaag gaggagtaaa caggagtgta caggtcagat ttattgtggc gttcaagtat
     3961 cttgtttagt gcaaactgaa tatttttttc tgtttcatca agaatagaat agctgacaaa
     4021 aactatctcc tgatttctgt tgattgctga taatatttcg tgtactatgt cctgtgatgt
     4081 tgcctgtgcc atgatcgtgg tataattaac ttagaattta gttatactat cggtataatg
     4141 ctaataatgc aacaattttt taattgcaac aatattgaca tttttatata ctgtgttaca
     4201 caataccacc ttataatact ctgatgaggt acactatgtt caatatgctt tttccatctg
     4261 tatcatctat tactgttata acgctattga gtttggtatg cggtatattg ctttcactgg
     4321 caaaagtact gctcaaggtt gaaaaggacc cgcgtattga gaaaattgta gaatcacttc
     4381 ccggagccaa ctgtggcgca tgcggttttc ctggatgcgt tggttatgcc acacatataa
     4441 ttgaaaaggg tgcatccatt aacctttgcc ctgtcggcgg tccggagagt gcacaaaaga
     4501 tatcacagat tatgggaata gaagctgaga tagtagcaaa acgtattgct cgtgtacact
     4561 gccagggtgg tattaacgaa acaaagatac gatttatgta tgatggtcct catagctgta
     4621 cggctgctca actggttatg ggcggtttca aggtttgtga atatggatgt ttggggttgg
     4681 gtgattgtgt ccgtgcatgc ctctttgatg ctatccattt agacaaacga gggttgccat
     4741 tggttgatgc agaaaaatgt accgggtgcg gaaactgcgt tgaagcatgt ccgcgtaata
     4801 ttataagcct tttaggtgaa aacattgatg tttatatgat gtgccgcaac aaacaaaaag
     4861 cgccggttat gaagttagga tgcaatgttg gttgcacagc atgccggcgt tgcgaaaagg
     4921 tctgtcgcga ggaagttttt aaggataatc ccgatgtgga tacagcaatt aaagtggttg
     4981 attttttagc tgttattgat tatgaattgt gcattaactg cggcaggtgt gccgaagttt
     5041 gtccgcagaa tgttatagat tttaaaaaag ttatggtaat aaagcgtgaa accaattgac
     5101 cagatgtctg gtgcagaaag ggctgcggca cttctggtag cattaggtcc agaagttgct
     5161 tctgagataa tgaagtttct tgatgacgag agtattcaaa aattagcttc ggagattgct
     5221 aaaatcgaag ttctgtcacc acaggaaaaa gaagatttga taggacagtt tttgcttgag
     5281 cttaaaaaaa ctcgtggtat aacatttggt ggccaaaacg tagcaaaaga aatattatat
     5341 gcagcctttg gacaggataa agctcaggat atcttaaaac gattaaccca taaggatctg
     5401 gaaaaaggtt ttggcttttt aaatgatatt gacccggagt taattgtaac atttttgcac
     5461 aatgaacatc cgcagacgat tgctgttacg ctggcatacc tgccggctgc aaaggctgct
     5521 gaggttataa agcttttacc aaccgatatt gccaaagagg taactttacg catggcaaag
     5581 atggaacgca cttcacctga tgcggtgctt gagatatcgc gcgtgctacg caaaaaatac
     5641 gatgagtacc gatatcaggg gcaatccagt tcaaaggcag gtggtcttaa tgcgctcatt
     5701 gatataatgt ctcatttaag tggtgaccag gaaaaacagc ttattgatta ttttgaaacc
     5761 accatgccgc aaattgctga tgagatacgt gcacgcatat ttacatttga aaatgtaatt
     5821 acccttacca atactgagat gcgcatcctc attgatgaaa tcaataatga ctatatgatt
     5881 gccagagcat tgaaaggagc aggtgatgaa ataaggttta agtttttccg caacatgagt
     5941 aaaaaccgtg caactgatat catgaatgag atggaagcca tggggcctgt gcgattatcc
     6001 gaaattcaaa gtgctcgcga caatattata agggtaatgc gcatactgca tgacaatggt
     6061 gttataagcc tcagaaaaga aaaagagaag tatattgaat aaaaaagaaa tcctttcatg
     6121 gcttcagcaa tatgggcttg ctcctaacaa aatgtatggg cagcattttt tgtttcatga
     6181 aaaatatatt gaggcgatag ttactgcaat cagtccttgc agcgaagatg tgatacttga
     6241 aattggtcca gggctgggag tgcttacgca acgattgagt gaacatgcaa agaaggtcat
     6301 tgcggtggag attgatgctg gatttgttga ggttttacaa tcaaggtttg ctaacaattc
     6361 cagagtaaca atcgtacata atgacttttt aaaaacaaat ccttacgatg aatgtactgt
     6421 actaaccggt aatcttcctt ataatatagc ttctcagatt atttttgcgg cagcacataa
     6481 ctttacagca ccccattgtt tttttttgct gcaaaaagaa atggcacagc gtcttatagc
     6541 aaaaccaaaa agtgccatgt attcagcatt tacggttggg attggattat attttactgt
     6601 tcaaacatta tttgatatac cgccgcaagc cttttaccct gagcctaagg tgtattcaac
     6661 atttgtaaag cttacccgaa aaaattctgt aatgcttcat actgatgaaa aagcggtgtt
     6721 tgagcagctt gtagcaactg cgttctgggc acgccgaaag atgcttaaaa cagcgttgaa
     6781 acgttcgccc tatacaacct accgcgaggc ttttatttca caggcttttg gccaattggg
     6841 ttttgatgaa aaggttcgtg gcgaagagct tacccttgat gaatttgttg cactggcaaa
     6901 gtactgtgca cactatggca ataaataaca agcaataagt aaatgggata tgataccaga
     6961 agaaacaata tataatctgc ttaccactgt gtcaaaaccg gcgcgatatg ctggtggtga
     7021 atggaatgca gttgtaaagc ctcatgcacg ggtaaaagtg ggattgtgtt ttcccgacct
     7081 ctatgaggtt ggcatgtcca acaatggcat tcagattttg taccatgcca tcaacggaat
     7141 gcaacatgcc gcagcagaac gtgtctttgc cgttgagcat gattttgaaa accagttgcg
     7201 gcagtcaaac atccctcttt acacgctgga aacctatacg ccgctgtgtg cgcttgacat
     7261 gcttggcttt aatgcagcat gcgaactttt atacaccaat attttacagg ttattgattt
     7321 aggcactatc cctttgtatg caaaaaatag gggcgatagt tatcctttaa tcattattgg
     7381 cggcgaggca atgagcaacc ctgcaccgat gctacctttt gctgatgcaa tatttgttgg
     7441 tgatggtgaa gaggcaatta tccggattgc gcaggtgctt attgataaaa aagataagca
     7501 cttattgaag gatgaggtgt tgacaaagat tgctgcgatt gaaggcgtac tggtaagccg
     7561 cgatgttgag tatgggtata caggcaatgc cattactgca ataaaagcgc cagcggtata
     7621 taaacgtgtg gcacggcgtt tccatgatta tgaggtaaca cgccctgtag tgccttcaat
     7681 tcgcagcgtg caggataggg tatccgttca gcttgacaga ggatgtaaaa atttgtgcaa
     7741 attttgtcat gctggttact gggaattgcc ctatcgtaag gttgaggtaa gcagggttgc
     7801 acaggctatt gttacaacac tgcagtcaac cggttttaat gatgtatcgc tgttttcgct
     7861 gagtgtgggc gattatacca atattgtcag tctgctcaat atcattgtgc cacaactcat
     7921 tgaacagggt gttagcatat cgttaccgtc gctgcgtgtt gatatgcaat ccattgctat
     7981 cattgaatca atttcagatt taaaaaagac agcactcacc tttgctgttg aatcagcatc
     8041 agatgcattg cgctatcgtg cttacaaacg gcttaccata gatgagatta tgaacatcat
     8101 tacagagctg tcacggcgtg ggtggcagcg tataaaactt tattttatga tagggctgcc
     8161 gggatgtgac gataccgatg aggcggccgc tatcattgca tttatgaagc aagcatgttc
     8221 cattgacaaa agaatgcgct acaatgtaac tatctcccca tttgtgccaa aagctcatac
     8281 tccatttcag tatgaaaaac agtatgatga gcactactgc cttgaggttg tcaataccat
     8341 caaacgcaat gtgccaaaaa atgtcacgat taaaaatcac gatgtgtatg catcgctcat
     8401 tgaaggtgtc ctggcacggg gcgacagtat gcttgccgat gccatcgctg gtgcatacaa
     8461 tgctggtgct cgccttgatt tgtgggggga gcattttgat tttgcactgt gggataatgc
     8521 tttacaaaca agctgtggca actggaggcg ctatttaaat aggcggtcag atcaagagct
     8581 tttaccctgg agtatgatac aaacgcgcta tgaaaggtta atagaaaaaa agaaggactg
     8641 cacatggaat ggcaggggtt tgcctcatgg gtttaaaaat gaaattctta acagggaagc
     8701 gtttgcacaa gctgcacagc aatttaccat gcgttatgcg ctggcagcca cattgcgtat
     8761 gcattatgca aaaaaaggaa aagcccgttt tattggacat attgatagca tggaaattgt
     8821 aaagcgagcg ttgcgtatgg cacatattcc tgtttcgtat acacagggat acaacaaaca
     8881 cgaacggtta ataacagggc cggcgctgcc gttagggtat gaaagttatt cagagtttgt
     8941 tgatgtacag ctatatgaaa gttgtagtgc aatgagcttg cagaagttac aggtatcatt
     9001 tcctgatgga tttgaagttg tcaatctata tcaccatgat gcatcaacgc tgccgcttaa
     9061 acgcattgtc tttgctgaat acctgctaca gtttaccgat aactatcggt ttaccttgtt
     9121 tatcaaccgt ttggaaagca aaaacgacat tgtaaaaaca acaaaaaaag gattgatgaa
     9181 tttgcgcttt gatgatgcaa tagccggtgt tattgttgct gatagcagta taaggcttgt
     9241 catggcgctg ggtgagcatg cgatacgccc tgatacgctt gttgctgatt ggtgtggtgt
     9301 acacaatcca ttaactattg ctgcaattac aaaggttgca tcgtattatt tgcataatga
     9361 tgaattaaaa aaatttcatt aatatacgag gtatgctatg ggttcgcttt catacaactt
     9421 agacgggaag gtagctattg ttaccggtgg tacacgaggc attggattgg ctattgcgca
     9481 ggaattgctt aaacaacatg caaaagtagt catctgcgct cgtaaacagg aagggcttga
     9541 cagggtaaag gagatgctta aagcaggcga cgacctttta acctgtgttg cacacatcgc
     9601 aaaagaggac gatgtcaacg cactggttga tgccgcagtg caaaaatatt ccactgttga
     9661 cattttaatt aataacgtag ggatgaacct tatgactggt agccttgttg atgttgagct
     9721 ttctgcatgg caaaagatta ttgattcaaa ccttaccggt acatttcttg tttcaaaaaa
     9781 ggttggggca attatgaggc agcagaaacg cggaaaaata gtcagtatat cttcagttgc
     9841 cggcaggctt gcatcgccgg gaatgaatgt gtatggtatt gctaaagccg gtgttgagat
     9901 gatgacaaaa aatttagcag tggaacttgc cccctttaat gttcaggtaa atgcagttgc
     9961 tcctggtatg gtgcgtaccg attttagcaa gccattctgg tcaaatgaag caattcacga
    10021 tatggtggtt aaaaatattc ccatgggaag aattgccgaa attgatgagg tggtgcatcc
    10081 cgttttgttt ttagcctctg atggcgcacg ctatatcacc ggtcaaacta ttgtgattga
    10141 tggcggagct tcaatactat agtcatgcac tatagagtat agtatcccga tgtagcttta
    10201 ttgcataatg atgacctgcc atgtgccatt gctggaaggt tttgctttca gcaataaaaa
    10261 tctgtgcatg ttgtattgtg cgtacgggta tacgctttcc atcaagcaac aactcatcct
    10321 tggctatttt atgattattg atgattattt ccttaacaat gtttggatta gttttgctgc
    10381 gtacacatgc aggtacccat ttgctatttg attgtataaa ccaatcccat cgcaggcagt
    10441 ctattgcaag ttcctctaca gggggatact cttccttaat aaaggtaagg atcatagttg
    10501 caattgcttc ccaatccttt gcaaacaaca cgaagttttt tttataaaaa aattgcgcaa
    10561 tcatctcaaa acttttaaaa ctatcgccgc tggttataaa atgacatagc atcatccatg
    10621 tagttttgaa atgatgatta taaaacctgt caaccacacg ttcaatgcaa tgaagagctt
    10681 gcatcatatg gcctggaatc cagttattct taatgacagt atagggcgct tcagcatcat
    10741 gctcaatacc aaattcattt ttttgtgatg caatctttgt gccgggtaaa atttttaaaa
    10801 atcccatctg aaaataatgg ggctttaatt caatgatttc attaaagctt tctttgattg
    10861 cctcaatgtc ttcatacggc aacccgcata tcatgtctaa atggatatgt atagtgttta
    10921 gttccataag ctttgctata ttttcttttg cttgttccca gttcatcttt cggtttatgc
    10981 tgctcagtgt ttttgggtga atggactgga taccaatctc aaattgaaaa tagtttttgg
    11041 gtacctgttg taataatgta aaatcttcat caaacagcaa atcagggtgt atttcaaaat
    11101 ggaaatgagt atggttattg ttgtgcgata gtaaatagta aaaaattgat cgagcatggg
    11161 tgggattgca attaaatgtt ctatcaacaa acttcaccat cctgaccctg tgctgtagaa
    11221 aaatgtcaag ctcagcatac acctttgata aatctctgaa ctgtattgat tgatcctccc
    11281 ttgaagagat gcaataacta cacgcatacg ggcaacccct gcttgattca tagtatataa
    11341 aacgggatgc tacattgtta atatcacttt cagaataggg gaagggtatt tcattaagat
    11401 ggtagtttgg taatgataca gtttttgtgt ctaaaagaaa ttgcttttgt gcagcgtact
    11461 caaatgatcg ttcacctgct ccctgtacga tagttgttat aaatgggaaa tgcttcagcc
    11521 attcatcagc tgtgtagctc acttcaggtc cacccagtat tattatacag tttggcaatg
    11581 ctgtatgtac cataggaagc aattttttta tgtaagttgc attccatata tacactgata
    11641 aacacaacac atccggatta atagtaacta tcgcattgcg tgtgtcatca acaggttggt
    11701 taatggttgt ttcatgaaga tataccgtat aattgagtgg ttgtaaaatt tgttttaaat
    11761 agtaaagggg cagcgaggaa tggatatatc gtgcattaac gccaagaagt aaaatggtta
    11821 aattcatata ataaaaaata attttgtaaa gaaaatcccg gtggtgtcac caggattttc
    11881 ttctgtatta ttatatcaat catatgattc tgaaccaaga taggtaaccg gtggaacatc
    11941 cataagccag cgcagggtat tctttaattt cataagctgt acaaacaggt catggattgg
    12001 ctcttcacca ggctcaaaca tggggctttt ccagtaaaat gatagccact cctgaatgcc
    12061 atacatggtt gcacgttgag ccaggtcaaa gaatagtaca aggtcaagta caaccggtgc
    12121 agccagaata gaatcacggc ataaaaagtt aacctttatc tgcatggggt aatccaacca
    12181 gccgcgtata tcaatattgt cccagctttc tttattgtca cgacgtggag gatagtaatt
    12241 aatgcgaaca acatgatagt agccgtcgta caattccgga tattcatcag gctggaatat
    12301 attatcaaga accgaaagct tgctaacctc tttcgttttg aatgattcag gatcatccaa
    12361 tacctcgcca tcacgatttc ctaaaatatt tgttgaatac cagccgtcaa caccaatcat
    12421 ccttgccttt aaaccagggg caataattgt tttcattaaa gtctgccctg ttttaaaatc
    12481 ccgtccaccc ataggcacct tgtttttctc ggcaagctgt cgtaaggcag gaatttcgtt
    12541 acagaggttg ggagcgccgt ttgcaaaagg aatacctaaa tccagcgctg catacgcgta
    12601 tatcatgctt ggggcaatat acttattatt ttctttcagc gctttttcaa aattttcaat
    12661 ggtatcatga acacctggtt tcatttcctg ataaatctca gtgctggcac accataccat
    12721 aacaacacga gataagtttt tttctttttt aaagttaagg atatcttctt taaccatctg
    12781 tgctaattcc cagtgtgaag gagccttttt agtccatgtc ccatggagtt ttttaacata
    12841 ttcattaaag aaaacggctt tccacggctt tacttttgat agttcatctt taatgggttc
    12901 aagatcttcc ttttgcagta caccagcatt gatagctgct tcatatgcat tatcttcaaa
    12961 aatgtcccat ccagcaaatt caatatcctg caaattggca agtggaacaa aatccttaat
    13021 gggaacataa tgtgcatcat cacccttgcc tatctttaac tttcccatca tcgaaacaga
    13081 acccttgggt gattttaatc ctttacggat aagcattaca cctgcaataa acgtggtgct
    13141 gacagcacca agcccgggta taacaacccc taatttacct tcagcttttt gaatctcagt
    13201 atttttaagt gtcaaggtac gcctcgcttt taatttaaaa aatttatgta aaaaataata
    13261 acatcctgca atagaacaaa aaaacatgct atttattaca aattactaaa cataactatc
    13321 atatccatag tcaagaaatt tatcgaaaaa aggcagatta ttaataacaa taaaacaata
    13381 atcccagatg gttggtaata tatttttttg ccattaaaca aacattatta taaatgtcaa
    13441 tagaaaaaaa tgacatgatg tttgaaaagt aaaaggcatc atgaaaggta aataaaacag
    13501 taataactat cgtttacctc ctaatagtga acctaaaatt ccccttacta tctgccgtcc
    13561 aacagagctc cctattgaac gagcagcact tttcatcata gcttcaacta cggtatcact
    13621 ttttctacca gctctttgtt tagggatatc ttccttctca tgagtagctt ctacagcttt
    13681 ttgtgcacga gtcttcagca tttcataagc tgattctctg tctattgttt tatcataaat
    13741 gcccttaacc ggtgatgcat caataatttc atttcgttct tgcggcgtaa tgggaccaat
    13801 ctgtcctttt ggtggtacaa tgaatgcacg ttccacaatc tctggtttac catcttcgtc
    13861 cagaaatgac actaacgctt cgcctacttc taattctata atggcttttt caatatctaa
    13921 ttttgaattt tccctgaatg tttgggctat tgctttcact gctttttgat cgttgggagt
    13981 aaaagcacgt aaagcatgct gaatcctgtt tcctaattga cccaatacag tttgtggtat
    14041 atctgcaggg ttttgggtaa tgaaatatac accaacaccc tttgaacgta ttaaacgaac
    14101 tacctgctca attttttcca gtaaagctct gggtgcgtcg ttaaaaagca gatgtgcttc
    14161 atcaaagaaa aaggcaatct ttggtttatc ccgatcgcca acttcaggta aaatctcaaa
    14221 aagttctgat aaaagccata gtaaaaatgt agcatagagc tttggagtgt ttataagcga
    14281 ttcagcagat agaatattta cataaccccg ccccgtttca tcagtctgaa taaaatcttc
    14341 aatgtttagt gcaggttcac caaaaaataa ttcagcgccc tgattttgca gctctaaaat
    14401 actacgctgt atagctccaa tacttgcagt ggatacccgt ccatactctg tattaaattt
    14461 tgaagcatta tcgccagcaa attgaatcat ggatcgtaaa tctttaatat ccagaatagg
    14521 caatccatta tcatcagcaa tcttaaatat tacattgagc gttccggttt gtgtctcatt
    14581 gagattcaaa atacggctta aaagcagcgg tcccatatca gtaacagttg tacgaatagg
    14641 atgccctgat tttccaaaca aatcccagaa aacaacaggg aatccttgaa aagtgtgatt
    14701 atcaatctta agctttttaa ggcgttgtgc aagtttatca tctggctttc ctggtttact
    14761 tatccccgaa agatcacctt ttacatctgc cataaacacc ggaatgccag ctaaactaaa
    14821 attttcggct ataacctgta acgtaaccgt tttaccggta ccggttgcac cagcaattaa
    14881 tccatggcgg tttgccatac gtggaatgat acaaaggtct ttttcacctt tagcaatgag
    14941 tagtggggca ttcatttctt tcctccaatg tgtctttatc attgtttgta gtattacctg
    15001 tacgatagtt actgtcaatt ctattttgct gtttcacata tcataggctg taataattag
    15061 attttgaggg tgcccctgcc ggaacaggta ttggaaaatt tttcttgttg atgtatgcaa
    15121 ccaaaacttt atttattttc tatctttgtt tcacaagaga aaaagggtct ttatgcgcct
    15181 gcattcaaag acgacgtgcc ttccgtggca cggctgttta ttgttgtgat gatggcagta
    15241 tcattctgaa ggagcgccag cgactgaaaa atctattttt caattttaaa aaaaatcgag
    15301 atactaatca tccggctcag gatgacagac gttaactttt tcaaaaggga ttaagatttt
    15361 tcactatgct caagatgtta tactctgcac ttttattcaa aaaagcatct ccatattttt
    15421 t
//
LOCUS       DAOOJH010000054        15148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3785_length_15148_cov_9.948784, whole genome shotgun sequence.
ACCESSION   DAOOJH010000054 DAOOJH010000000
VERSION     DAOOJH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15148
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3785_length_15148_cov_9.948784"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1006
                     /locus_tag="PLJ75_08070"
     CDS             347..1006
                     /locus_tag="PLJ75_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05207.1"
                     /translation="MKYFNFISSTMVLCLCLAIFTSPHAQENTTKQFNPLYDAEAVIQ
                     RLSYDNFKNIKLLRTAIYNFGGGEQEFNSLVDTYAEASALYFRNEMVASANLFTRNEK
                     DIKEVALRLAKIYKDQSEKLHINVIKMGVRHSLKASIEQAKPNPSVEPLISNASYAIK
                     SANDYLIRSKPVDAIYYFRRAKENCFKVYQILGEQLPEEYKKDVVDNQNKIYIAKEKK
                     N"
     gene            complement(1007..1792)
                     /locus_tag="PLJ75_08075"
     CDS             complement(1007..1792)
                     /locus_tag="PLJ75_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD05208.1"
                     /translation="MIGFYEKRIFSYCISLCLIILCCSSVKPLTRSDTILSYYEEGYN
                     SNNWKTRVEAIKAVSSIKNERAENLIIKALNDSHNAVRIEALQILIQRPIKRARPIIR
                     DIALQDSNDNVRWSALMALSQFRDPRDASVFISNCANGDWLIREAAIVGLLSIDDFST
                     KYVNVDVIVRALHDSSISVIMATLRNLNIRHTQIYYTLIGMLKEKNNIMPSLTIAILN
                     ALQLYRLEENDKEVVINFLTHPNSQVRLAAFHTLKRQNQMNNM"
     gene            complement(1761..2453)
                     /locus_tag="PLJ75_08080"
     CDS             complement(1761..2453)
                     /locus_tag="PLJ75_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPD05209.1"
                     /translation="MQRLIITVMAILLLMSINSFAKPVGKILELSGDIDITSMITGKR
                     IIPEEGTVIDGNDKIRTGRKSFVSIVLNDGSKLFIREISVLYVQDIKLKLTDQPTRIQ
                     IITGKLRVIAAKTMKGNSLVVYTPTAIAGVRGTDFGIIATQLETRVVIFQGNVEVANK
                     DKNILKSYILKDREETKVTYQQPPEAPVVVPDYILSKWFDTYSIDEKKNIIIKRKQED
                     GLLDRLLRKKDF"
     gene            complement(2533..4842)
                     /locus_tag="PLJ75_08085"
     CDS             complement(2533..4842)
                     /locus_tag="PLJ75_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPD05210.1"
                     /translation="MNSRCIALFRIIGFVFIFSFISCTNVVLPQGLSQLGFYGDSVSI
                     IQTLEKKQKLSYTEHFMAGIAYKKNGDYKKAMYHFANSCFAQRQALTLTIYPQPVYSF
                     VSRLSTRSPLYNDAVYEIAKGFMYYQEYNYVLKFIDLIKKEKTGVYRDAQMLKSDALI
                     QLKKFEQAKETIEKTISQYHDVDSLVGLYLKLASLYEKQNSYLYAVQSYIKLLTISTK
                     SWRSQIAAKRILQIKEKNPDIVLLPDDIIMVVEALYNTKNYSDIVSLDSTWDKFFDNA
                     DIRYEFDSIRIKALCQLGKTGEIKQIYSQYKNSDFWDDCAVVIVRALWNSKKGEAISW
                     VNKLAETTDDGLKKTALYYKALYSIYRKEKNSQPVDEFVANYPNDALSQELLWLYAKN
                     NITSNKVAQYYLEKYYSMFGVKGKYADEVYFWLYILNSKENPEKAQTILYDLIAYRPD
                     SAYSWILLERRSPDYTVDHLSLDFSMAIQTLNFSTTLFTHAMLTYKERDEAKRLKRAA
                     LIKKSFSKVSELQYDKIPKLNEKLSLQDISKNNFVRIEHYFSIGYLEGINREFSVFAS
                     NSNNDVYIVSYLLGLRYNNYFMSAQALVQLLRRMNLTENIFLMSPDAINSLLPVPFDT
                     CINSAAKKYAVQEPFICAVIKAESLFNPYAESSAGAVGLMQLMLPTAKDIARKTGYGT
                     ITKDVLREPCISIEMGTYYIAWLMKNLNNNITLVAAAYNAGIGNVLKWKHDSEDLFTI
                     SVPFAETKGYIERIKKFYYQYMLLYDIRK"
     gene            complement(4845..5684)
                     /locus_tag="PLJ75_08090"
     CDS             complement(4845..5684)
                     /locus_tag="PLJ75_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05211.1"
                     /translation="MRRRIHFFMCSFLVICATLCGYHDDGFSQRTKSSQQAGEQRKAE
                     KGLKDNERFFYYINFSVSNSVVEQERLVCRKAILYDMFARFLYMKFRFNDAYKQIRKS
                     QELLIGLYLMVLSREIEEGKKLLDSVSPGPIMKNDYVSKHYCNLGYTNNEQAKLYMVM
                     ADNYRTSLYSMRLYKYIQALKKAKQAKRYAIIAYVTFQDKEFKKKFTIKDKGNNEPYI
                     YSYLKTKLEAIQDENEKKYLIPILGDSYYQLNNDYSYYDDVWSNGSLHELPEYAEYEK
                     EEE"
     gene            5803..6786
                     /locus_tag="PLJ75_08095"
     CDS             5803..6786
                     /locus_tag="PLJ75_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HPD05212.1"
                     /translation="MKKTTIISLLSIVCLTLLITCTSFDPAMFQKKPTIQFHSFQFKE
                     ITLNDMTTLFSIEIENPYPVTLYVDSIQSQIDIDSHTLLSLQSKDSLKLSKHSKAIKT
                     FAVTITYQQLLQAVNNYINRDSIMLSIKGTIGFSLPPSMQIVKPINVPFTIKQEIPTI
                     KPSIDIAHFKIIPPTLNELATMVKNRTLTISMLELNQVFADIQAFSQGKSIPDSILQL
                     DIPLSMSFDIIIKNKTKALLTGKSIDYNFILYDTEIAKGKPTIQSVNGTSTINCLTVL
                     STKNLSKGIVTALPKKNIHYMMKGSMLLDASFKDSTMPIPFSINHDGTISW"
     gene            7002..8072
                     /locus_tag="PLJ75_08100"
     CDS             7002..8072
                     /locus_tag="PLJ75_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPD05213.1"
                     /translation="MDVIPVILAGGAGTRLWPLSNEQKPKQFHNITGEGTLLELTINR
                     LKPLHPDFCLIVTARMYEDMSYEELHKTGLKGSIISEPMPRNTAPAILYAATYLSKLY
                     DDAIMLILPADHYIKKTDLFIQVLQKAIQQANNNYLVTIGITPTYPETGYGYIKAYDD
                     PSEVKPVEMFVEKPNLETAKKYLREGNYFWNSGIFVWKISVILDYYQKLMPQLYNAFQ
                     PLRDLTVEQLTQNDDLPWEIKRTVFSEIESISIDYGIMEKADYRYVIPGEFGWADLGS
                     WASIDDILNPDSNMNRSSSSKQVIFHLSKNCTVFPDKKLIAVVGLNDAVVVDSGDEIL
                     VMDKNASQDLREVVNHIKSKPQ"
     gene            complement(8066..8782)
                     /locus_tag="PLJ75_08105"
     CDS             complement(8066..8782)
                     /locus_tag="PLJ75_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05214.1"
                     /translation="MNKQVSLCQPGNGKGCCVCCGLFNLRNVSHNVLSQFLTEASIEV
                     SYGKIINNLQLRKYGSIKKIYDFRDSTTYVCPFMGFIFNNTPGCLVHPSINDGIDSRD
                     HSLFGKELCNDYFCPAYRILSDREKKILIRCTCDWYSYSIGIMDPLSFRWIIQQVEGI
                     LDIQIVPEIEQENAIVRCVNEALSMLGMVLNTLDAPLFYYSEPEYYLHMRIVSLNSES
                     MLNKNVREAIQRLIINNTRY"
     gene            8964..9608
                     /locus_tag="PLJ75_08110"
     CDS             8964..9608
                     /locus_tag="PLJ75_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPD05215.1"
                     /translation="MNYHDNLYELLEVTPSSSKNEIRKAFCSKVKMLHPDTTGSSSER
                     FTLELQKIIEAYRILMDDTQRARYDATFFKQAAKPLKYYIPQKRVRYSISLKDLLKNQ
                     FLPKKMRRKDIIHKFDHDIEIVCTQQELHKGAIALVQLPSRILCPLCNGQHRDCYLCN
                     GLGRIASPSTFEIELPGDLTKTTIITVDLSNYRPDTFTNLTVKFLRIKITIISP"
     gene            complement(9598..10896)
                     /gene="hisD"
                     /locus_tag="PLJ75_08115"
     CDS             complement(9598..10896)
                     /gene="hisD"
                     /locus_tag="PLJ75_08115"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPD05216.1"
                     /translation="MLQIERLSELTKDDLYKLFQRFGADFTSIMVNTVIPIVQRVREE
                     GERAVLAFTKQFDGVALSSVYATIEEIAQSYEKTDTNVIDSFKKAIDNIKEFHLHQKR
                     DSFLYTRDDGITLGMLYQPIDSAAIYVPGGKASYPSSVLMGVIPAQIAGCKNIEIITP
                     PANDGSIPAYIGAMAYLLGITTIIKSGGAQGIALAAYGSGAIPKADIVVGPGNIYVTA
                     AKAYLFSQGIIQIDSLAGPSEVLVIADDSAQPKWVAWDLLSQAEHEEMAIAILVTTSE
                     RLAKQVIEYLDEDLSSGRGRSEIKRKAMQHARILLVSSVEEAIDFSNNYAPEHMEFIV
                     KNPMDYLTQIKNVGSLFLGAYAPVAVGDYYSGTNHVLPTGGAARFSSGLGVETFMHRT
                     SFQYLTPDGLRDAVPHVCTMSLVEGFDDKHGDSLTIRFKG"
     gene            11022..11288
                     /locus_tag="PLJ75_08120"
     CDS             11022..11288
                     /locus_tag="PLJ75_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05217.1"
                     /translation="MIKHCVIALVILSLLYPVACKKSTITPKLFVTIQNEMLESDMQP
                     ATKESIVAKYNLSLKEYEEYEKKVESDPQLKADVGKERLNIMKQ"
     gene            complement(11289..12110)
                     /locus_tag="PLJ75_08125"
     CDS             complement(11289..12110)
                     /locus_tag="PLJ75_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD05218.1"
                     /translation="MIIDSHAHIFTDDMIVHKDRYCDDKQFALLYYGKAKIANDALLI
                     DSMQNNGVDASVALGFTWQRNDYAQRHNEYMAMVQERHKGKVYAFGAIALDTDMYQQI
                     KNIKQMGLYGIGEVAFYVNGFTEEEETIVSNILAIAGDMELPVCLHVNEPVGHIYTGK
                     YTTDMQRLYALLSQYTNTTVICSHWGGGLLFYELMPEVKKSLQHVYYDSAASPFLYSD
                     TIYSIAITICGKEKILFGSDFPLLDYSRYNNSIQLLGENAQWIADQNVKKLLRLL"
     gene            12191..13687
                     /locus_tag="PLJ75_08130"
     CDS             12191..13687
                     /locus_tag="PLJ75_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HPD05219.1"
                     /translation="MYNIEYFLEQAKENFHTLRVIGEDGNTKYIHSKYYPTREASYLK
                     DIVNPEKYDVLIILGIGLGYHLAYLTEIIHSFTHIICIDINNYLTNACIPEHIHLLLQ
                     NPAIETIIIDPLMPEQLYKLLSINCSKGIQVIDHQASFAAFPSAFASIKNIVQQIIQK
                     SLSNQLTTNYFAARYCKNAIMNIPPLPTCYPVIQCNNMYHNIPCIIVAPGPSLEESLP
                     LLYKNQNKVYIISVDSAVKVLSSYSIEPDYIITIDPQPIVYAHLFGVHHHAIIVAMLT
                     AHPAVFSSPVIVSLNSHPLCQLIEQVFPATIGSIDSRTGTVLGDAVSFAQFSGFSPIG
                     IAGADFSFPHFITYVRSTEYHYRYAAAHTRLSPIETSNMQYIFQASKGTLVEQRYSRK
                     VFIQYRNTIDALIYNTITYQINVSGLQLKNAVPLSTEDFFTLPAIMHYHKKLSPQALK
                     QPMDIHWERLYSYLYNDSIFNELMKASKVGDSYASQLKLLLKLLLQKR"
     gene            13692..14282
                     /gene="coaE"
                     /locus_tag="PLJ75_08135"
     CDS             13692..14282
                     /gene="coaE"
                     /locus_tag="PLJ75_08135"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPD05220.1"
                     /translation="MRIGVTGIYASGKGTVCSMFEQLGATVIDTDIIAREIVTPPSPV
                     LDSLVATFGRDILNPDGTLNRRYVANMVFNDANKVNALNAITHPAILCEVLQRTSNHS
                     TIYMVNTPLLFETEFYTYMDKTIVVIANQQQVVKRGQMRDGITEREINERLQHQISLN
                     EKMKLADYIIDNSKSLENTKKQVEELWKILISMKNQ"
     gene            14249..15031
                     /locus_tag="PLJ75_08140"
     CDS             14249..15031
                     /locus_tag="PLJ75_08140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPD05221.1"
                     /translation="MENFDFNEKPVAKEKNVFLLHLDIPRIILICAVIIGIVVTTFLL
                     GMNFSENKVVSNTALVSPDIPNDTLAQLDKGIDLGTSPQNPDFSTLDNAPDSLTDTLP
                     RVEEPKTYTENVTNKEANPGFSLANNDILSTQIIEQKEMKKTTPVKTTSKKNTATTKK
                     TVKKKQIVEVAQTEEIPSKNKAHRSWSIQVASYNTIFKAQKEAKYLHSMNYDAFVDKA
                     DISGKTYYRVKIGPILKEDKAIGMLQEIQSIDKYKESYIVKE"
BASE COUNT         4932 a         3027 c         2401 g         4788 t
ORIGIN      
        1 atcttcattg tataatcata gtgtttacca ctatatatat atgcaccacg tatcatagag
       61 aagccatcct tgtagtttta gtatgtatca aaactataca acaacatgta gcatgtaaca
      121 catgcttatg gcaacaacat actatcgcat tgtcctcact ataaatccaa taaaaaattg
      181 aataatagca atgtttcatt aataccgcac aaatctctct atttcgtcaa cctgtttttt
      241 aaatagtaac gataacattt tacttgactt caacatttaa tctattatat gattccatac
      301 ataatacaaa tttaaagtat ataagtctta taaaggagat agcaacatga aatatttcaa
      361 ttttatttca tcaaccatgg ttttgtgctt atgtttagct atatttacat cacctcatgc
      421 acaggaaaac actacaaaac aatttaatcc attatatgat gcagaggctg taatccagcg
      481 cttaagttat gataatttta aaaatataaa attattaaga acggctattt acaactttgg
      541 aggtggcgaa caggaattta atagtcttgt tgatacctat gcagaagcca gtgcactata
      601 tttcagaaat gaaatggttg cttcggctaa tctctttacc aggaatgaaa aagacattaa
      661 agaggtagcc ttgcggttgg caaaaatata taaagatcaa tctgaaaaat tacatattaa
      721 tgtaattaaa atgggagtgc gtcattcgct gaaggcatcc atcgaacagg caaaaccaaa
      781 tccctctgtt gagccattaa tcagcaacgc aagttatgct attaaaagtg caaatgacta
      841 tcttatacga tcaaaaccag ttgatgctat ttattacttc aggagagcta aggaaaactg
      901 tttcaaagta taccagatat taggagagca gttgcctgaa gaatataaaa aagatgttgt
      961 agacaatcaa aataaaattt atatagcaaa agaaaagaag aattagttac atattattca
     1021 tctggttttg tctcttcagg gtatgaaatg cagctaatcg cacctgcgag ttggggtgtg
     1081 ttaaaaaatt aatgacgact tctttatcat tttcttctag cctataaagc tgtaatgcat
     1141 ttaatattgc tatagtaagg gatggcataa tattattttt ttcttttaac atgccaatca
     1201 gggtgtaata gatctgtgta tgtcgtatgt tcagattacg taatgttgcc ataataacac
     1261 ttatagacga atcatgtaat gccctgacaa taacatcaac attaacgtat tttgtagaaa
     1321 aatcatctat actgagtaag ccgacaattg cagcttccct gataagccag tccccattgg
     1381 cacaattaga gataaaaact gaagcatcac gggggtccct aaattgagaa agtgccatta
     1441 atgcactcca ccgcacatta tcattgctgt cttgtaatgc aatatccctg ataatagggc
     1501 gtgccctttt gattggcctt tgaataagga tttgcaatgc ttcaatcctt acagcattgt
     1561 gcgaatcatt gagagcttta ataataagat tttctgcccg ttcattttta atactggaaa
     1621 cagcttttat tgcttctact cttgttttcc agttgttact attatatccc tcttcatagt
     1681 acgacaaaat agtatcagat cttgttagtg gtttaacaga actacagcat agtattatta
     1741 aacataatga tatacaataa ctaaaaatcc ttttttcgta aaagccgatc aagtaaacca
     1801 tcctcctgct tccgctttat gatgatattc tttttttcat caatactata tgtatcaaac
     1861 cattttgata aaatataatc aggaacaaca acaggagctt ccggcggctg ttgatatgtt
     1921 accttagttt cctctctatc ttttaaaatg tatgatttca aaatgttttt atctttgtta
     1981 gctacctcaa cattgccctg aaaaattacc acccgtgttt ccagctgtgt tgctataatc
     2041 ccaaaatcag ttccccgtac accagcaatt gcagttggtg tatatacaac taagctgtta
     2101 cctttcatag tttttgcagc aataacccgc aatttcccgg tgattatctg aatcctcgtt
     2161 ggttgatcag ttaactttaa tttaatatcc tgcacatata gaaccgaaat ctctcgtata
     2221 aacagtttac tcccatcatt taatacaata gaaacaaatg attttctgcc agttcttatt
     2281 ttatcatttc catctatgac agtcccttct tcggggataa tccgtttacc tgttatcata
     2341 gaggtaatat ctatgtcccc ggataattcc agtattttcc caacgggttt tgcaaaagaa
     2401 ttaatactca ttagcagaag aattgccatc acagttataa ttagtctttg catcacaaac
     2461 ctccagttat aatatacact ttgactataa tttataatat agtatcaatt atgcaaaaaa
     2521 ggaaaataat tattattttc taatatcata caatagcata tattgataat aaaatttttt
     2581 tatacgttca atatatccct tcgtctcagc aaatggtaca ctgatagtaa ataaatcttc
     2641 actatcgtgt ttccatttca aaacattacc aattcctgca ttatatgcgg ctgcaaccaa
     2701 tgttatatta ttatttaaat ttttcattaa ccatgcaata taatacgtcc ccatctctat
     2761 ggaaatacag ggctctcgca atacatcttt tgttatagta ccatatcctg ttttcctggc
     2821 aatatctttc gcggtaggga gcataagctg cataagacca acagccccgg cagacgattc
     2881 tgcatacgga ttaaataatg actcagcctt aataacagcg cagatgaaag gctcctgaac
     2941 tgcatatttt tttgctgcac tatttatgca tgtatcaaat ggcacaggta ataaactatt
     3001 gatagcatca ggtgacatta aaaaaatatt ttcagtcaga ttcatccgcc ttagcaactg
     3061 caccaaagcc tgcgctgaca taaaataatt gttatagcgc agccctaata aataggatac
     3121 aatatataca tcattattgc tgttactggc aaatactgaa aattctctgt tgatgccctc
     3181 cagataccct atgctgaaat aatgttcaat cctgacaaaa ttatttttag aaatatcctg
     3241 taaggataat ttttcattca atttcggaat tttatcatat tgcaattctg aaactttact
     3301 aaatgatttt tttataagag cagccctctt taaccgtttt gcttcatctc tctctttata
     3361 ggttaacatt gcatgagtga aaagcgttgt tgaaaaattg agcgtttgaa tagccataga
     3421 aaaatcaaga ctcagatggt caacagtgta atcaggtgat cgccgttcca gcaatatcca
     3481 gctatatgca gaatctggtc tatatgctat taaatcataa aggatagtct gggctttttc
     3541 aggattctct ttgctgttta gaatatataa ccagaaatag acttcatcag catatttccc
     3601 cttaacacca aacattgagt aatatttctc taaatagtac tgtgcaactt tattgctggt
     3661 aatattattt ttggcatata accataacaa ttcttgtgac agagcatcat taggataatt
     3721 agctacaaat tcatctactg gctgtgaatt tttttctttt cgatatattg aatacaaagc
     3781 cttataataa agggctgttt tttttaagcc atcatccgtt gtttcagcta acttatttac
     3841 ccatgatatg gcttcaccct ttttagaatt ccagagggcc cggacaataa ctacagcaca
     3901 atcatcccaa aaatcactat ttttatactg agaatatatc tgctttattt caccagtctt
     3961 acccaactgg cataatgctt taattcttat gctatcgaat tcatacctta tatcggcatt
     4021 atcaaaaaat ttatcccatg tgctgtcaag tgatactata tcactataat ttttggtatt
     4081 atataatgct tcaacaacca taattatatc atccggcaac agaacaatgt caggattttt
     4141 ttctttaatc tgaagaattc tttttgcagc aatctgagac ctccagcttt tagtacttat
     4201 agtaagcagc ttgatataac tttgcactgc atacaaatag ctattctgtt tttcatacaa
     4261 tgacgctaat ttaagataca gccccaccag cgaatcaaca tcatggtatt gtgatatcgt
     4321 tttttcaata gtttctttag cctgctcaaa tttctttaat tgtatcagtg catcggattt
     4381 aagcatctgc gcatcccggt atacaccagt tttttctttt tttataagat caataaattt
     4441 aagcacgtag ttatattcct ggtaatacat aaatcctttt gcaatttcat acactgcatc
     4501 attatacagt ggtgagcgag ttgataatct gctaacaaag ctatagacgg gttgaggata
     4561 gatagttaag gttaaagcct gcctttgagc aaaacagcta ttagcaaagt gatacatagc
     4621 ttttttataa tcaccatttt ttttgtatgc aatccctgcc ataaaatgct ctgtatacga
     4681 cagcttttgt tttttctcca atgtctggat gatacttaca ctatccccat aaaatcccaa
     4741 ctgtgataaa ccctgtggta acacaacatt agtgcacgag ataaatgaaa atataaaaac
     4801 aaacccgatg atacggaata atgcgatgca cctgctgttc ataattattc ttcctctttt
     4861 tcatattctg catattctgg caattcatgc aatgaaccat tgctccatac atcatcataa
     4921 tatgaataat cattgtttaa ttgataataa ctatcgccaa gaataggtat taaatatttt
     4981 ttttcatttt catcctgtat ggcttccaat ttagtcttta aatacgaata gatgtacggt
     5041 tcattattcc ctttatcttt tatagtaaac ttttttttaa actctttatc ttggaatgta
     5101 acatacgcta taatagcata ccgtttagcc tgttttgcct tttttaaggc ttgaatatat
     5161 ttatacagtc gcattgaata taacgatgta cgatagttat cagccatcac catgtatagt
     5221 ttagcctgct cattattagt gtaccctaaa ttacagtaat gttttgacac atagtcattt
     5281 ttcattatag gaccgggtga tactgaatcc agcaattttt ttccttcttc tatttcgcgc
     5341 gacagtacca ttaagtataa tccaataagc aattcctgag attttcgtat ttgtttgtat
     5401 gcatcattaa atcgaaattt catatacaaa aagcgggcaa acatatcata gagtattgct
     5461 ttcctgcaaa ccaaacgttc ctgttcaacc acactgttag aaaccgaaaa attaatataa
     5521 taaaaaaatc tttcattatc ttttaaaccc ttttctgctt tcctctgttc acctgcttgc
     5581 tggctgctct tcgttctctg ggaaaaacca tcgtcatgat atccacacaa tgtagcacag
     5641 atcacaagga acgaacacat gaaaaaatgt attcttcgtc tcattattat acctttgttc
     5701 attttctttt gggcatttat ttttatcata ttttatgatt gactgtacac tctttttgat
     5761 acaaaatagt ataaaattat agtaaaaatc aggaaaacaa caatgaaaaa aactaccatc
     5821 atatccttat tatcgattgt ttgtttaaca ttacttatca cctgtacttc atttgaccct
     5881 gcaatgtttc aaaaaaaacc taccatacaa tttcatagtt tccaatttaa agaaattact
     5941 cttaatgata tgacaactct tttttcaata gaaattgaaa atccttaccc tgtcacattg
     6001 tatgtagatt caattcaaag tcaaatagat attgattcac atacattgtt atcattacaa
     6061 tctaaagatt cattgaaact atcaaaacat tctaaagcaa tcaagacatt tgcagtaact
     6121 ataacctacc aacaactctt gcaggctgtt aacaattata ttaatcgtga ttccataatg
     6181 ctgtcaatca aaggaactat tggtttttcc ttaccaccat caatgcagat cgtaaaaccc
     6241 attaatgtcc cttttacaat aaaacaggaa ataccaacca tcaagccatc aatagatatt
     6301 gcacatttta agattatacc gccaacctta aatgaacttg ccaccatggt gaaaaaccgt
     6361 actcttacta tcagtatgct ggaactcaac caggtatttg ctgatataca ggcgttcagt
     6421 caggggaaaa gcatacctga tagtatattg caattggata ttcctctatc catgtcattt
     6481 gacattatca tcaagaataa aactaaagca ttgcttaccg gcaaatctat tgattataat
     6541 tttatattgt atgatacaga gatagctaaa ggtaaaccta caatacaatc agtgaacgga
     6601 acctctacaa tcaattgttt aacggtactg agtacaaaaa atttaagcaa aggcattgtt
     6661 actgctcttc ctaaaaaaaa tattcactac atgatgaagg ggtcaatgct attggatgcc
     6721 agtttcaaag attcaactat gccaatacca ttttcaatta atcacgatgg aactataagc
     6781 tggtaatagt gcattataat ggttattgac aattttaata attgacgtat agaacaaata
     6841 ataacattat gccatagagg aatttctaat aattagattc aggacattcc cttgtgggta
     6901 catgtagttg aacaaagttt tgtcatagtg tgaataatga taatcctgat ttttacaggt
     6961 gcccatacgg ttgaccgtaa ttactgcagg cgaggttatt tatggatgtt atacctgtca
     7021 tattggctgg tggtgcaggc acacggttgt ggcctctcag caatgagcag aaacccaagc
     7081 agttccataa catcaccggt gaaggcacgt tattggaatt aaccatcaat aggttaaaac
     7141 ctttacatcc tgatttttgt ctcattgtaa ctgcccggat gtatgaagac atgtcatatg
     7201 aagagttgca taaaaccggg ttaaagggaa gcattatatc agagcccatg ccaaggaata
     7261 ccgcccccgc tatcctttac gctgcaacct atctatcaaa gctttatgat gacgctatta
     7321 tgcttatcct gccggccgac cattatatta agaaaaccga tttatttatt caagtactac
     7381 aaaaagccat tcagcaggca aataacaatt atttagtaac tatcggcatc actcccacct
     7441 atccagagac cggatatggg tatattaaag cgtatgatga tccatcagaa gtaaaacctg
     7501 ttgaaatgtt tgtggaaaag ccaaaccttg aaacagcaaa aaaatatctc agagaaggaa
     7561 attatttctg gaacagtggt atctttgtat ggaagatttc agttattctg gattattatc
     7621 aaaaacttat gccacaattg tacaatgcct tccagccatt gcgtgatcta acagtggaac
     7681 agcttacaca aaatgatgac ctgccatggg agattaaacg tactgtattt tcagagatag
     7741 aatcaatatc aatagattat ggtatcatgg aaaaagccga ttatcgctat gttatacccg
     7801 gcgaatttgg ctgggctgac cttggtagct gggcttccat tgatgatata ctgaatcccg
     7861 atagcaatat gaaccgttca tccagctcaa agcaggtaat attccatctt tcaaaaaatt
     7921 gtaccgtttt tcctgataaa aagcttatag cggttgttgg tctcaatgat gctgttgtgg
     7981 tagattcagg tgatgaaata ctggtaatgg ataaaaatgc atcgcaggat ttacgcgagg
     8041 tagtaaatca catcaaaagc aagcctcagt agcgagtgtt gtttataatc aaacgttgta
     8101 tcgcttctct cacattttta ttaagcatag attcgctatt cagcgacact atacgcatgt
     8161 gtaaataata ttcaggctca ctataataaa aaagcggagc atccagagta ttcaatacca
     8221 tcccaagcat tgatagtgct tcattgacac agcgcacgat agcattctct tgttctatct
     8281 caggaactat ctgtatgtcc agaatacctt caacctgttg aatgatccac ctgaagctta
     8341 aaggatccat gataccaatg gaatagctat accagtcgca ggtacaacga atgagtattt
     8401 tcttttccct gtcgcttaga attctatacg cagggcaaaa gtagtcattg catagttctt
     8461 tcccaaaaag gctatgatcc ctgctatcga tgccatcatt tatagaagga tgcaccagac
     8521 atcccggcgt gttattaaaa ataaaaccca taaacgggca tacataggtt gtactgtctc
     8581 tgaagtcata tatcttttta atgctgccat actttcgtag ttgcagatta ttgataatct
     8641 ttccataact gacttcgata gaagcttctg ttaaaaattg agacagcaca ttgtgtgata
     8701 cattgcgcaa attgaataga ccgcaacaca cacaacatcc tttcccattc ccgggctgac
     8761 aaagcgatac ctgtttattc atacgacacc ataatctcta ttaatttgta cccagcagtt
     8821 ttattactat atcactatct ggtgtgcaca aatacataaa aattcacgta ttaggtataa
     8881 ccgttataat ttaacttgca atgaatgcat ttcatgagta tactgtacta tacagtttta
     8941 aaatattctt atacatcggc attatgaact atcatgacaa tctctatgaa ttattagagg
     9001 taacaccctc ttcatcaaag aatgagattc gtaaagcatt ctgctcaaag gtcaagatgc
     9061 tccatcctga tacaacagga tctagcagcg aacgctttac ccttgaactt cagaaaatca
     9121 tcgaagcgta ccgaatcctc atggatgata cacagcgtgc gcgctatgat gctacctttt
     9181 tcaaacaggc tgccaaacca ctgaaatact atattccgca aaaaagggta cgctattcca
     9241 tatcattgaa agaccttctg aaaaaccagt ttctcccaaa aaaaatgcga cgcaaagaca
     9301 tcatccacaa atttgaccat gatattgaaa tagtttgcac tcaacaggaa ctgcataagg
     9361 gggcgatagc tctggtgcaa cttccctcgc gtattttatg ccctctctgt aatgggcaac
     9421 accgtgactg ctatctctgc aatgggttgg gacgtattgc aagcccctca acatttgaga
     9481 ttgagctgcc cggcgactta acaaaaacta ccatcattac tgtcgatctt tccaactatc
     9541 gccctgatac atttaccaat ttaaccgtaa aatttttaag gattaaaata acaatcatca
     9601 gcccttaaat cggatagtaa gtgaatcacc atgtttatca tcaaaccctt caaccagcga
     9661 catggtacac acatggggta ctgcatccct taatccatct ggtgtaagat attgaaatga
     9721 tgtacggtgc ataaaggttt caacgcccaa tcctgaagaa aagcgagctg ccccacctgt
     9781 tggaagaaca tgattggttc ctgaatagta atcgcccact gccacaggag cataggcgcc
     9841 taaaaataat gaaccaacat tctttatctg tgtaagatag tccatgggat tctttacaat
     9901 aaattccata tgttcaggag catagttatt ggaaaaatct attgcttctt ctaccgatga
     9961 aacaagcaat atccgtgcat gctgcattgc ttttctctta atctcactcc ttcctcgtcc
    10021 ggaggacaaa tcttcatcga ggtattctat tacttgtttt gccagccttt ccgatgttgt
    10081 taccaaaatt gcaatggcca tttcttcatg ctcagcctgc gacagtaaat cccatgccac
    10141 ccactttggc tgagcactat catcagcaat aacaagcacc tcacttgggc ctgccaggga
    10201 atctatctga ataatgccct gactgaatag ataagccttt gctgccgtca catagatatt
    10261 ccccggacca acaactatat cagcttttgg tattgctcca ctgccataag cagctaaggc
    10321 tatcccctgc gccccacctg atttgatgat ggttgtaatc cctaaaagat atgccatcgc
    10381 accaatatat gcaggaattg atccgtcgtt agccggtggt gttatgattt caatattttt
    10441 acaccctgca atctgtgcag gaataactcc cattaagact gaagatggat atgaggcttt
    10501 accaccggga acataaatgg cagcgctgtc gataggctgg taaagcattc ccagggtgat
    10561 accatcatca cgggtatata aaaaagaatc tcttttctga tgtagatgaa actctttaat
    10621 attgtctata gcctttttaa acgagtcgat aacatttgta tcagtctttt catatgattg
    10681 cgcaatttcc tcaattgtag cataaacaga ggataatgca acaccatcaa actgtttggt
    10741 aaatgcaagc acagccctct caccctcttc acgcaccctc tggacaatgg ggattacagt
    10801 attaaccata attgatgtaa agtctgcacc aaagcgctga aaaagtttat atagatcatc
    10861 ctttgttaac tctgatagcc tctcaatctg taacatatat acctctttta atctttgttt
    10921 agtgttattt tacattcatt attatagata atgtcttgac tatataatta attattatta
    10981 tactacacaa acattgccgt aaaaatattt atggagggtc tatgataaaa cattgtgtaa
    11041 ttgctcttgt aatattaagt ttactctatc ctgttgcatg taaaaaatca actattactc
    11101 caaaactttt tgtgaccatt caaaatgaaa tgcttgaaag tgatatgcaa cctgcaacaa
    11161 aagaatcaat cgttgcaaaa tacaatcttt ccctgaaaga atatgaagaa tatgaaaaaa
    11221 aagttgaaag cgatccccaa ttaaaagctg atgttgggaa agagcgtctc aatattatga
    11281 aacaatagct ataaaagtct caacaatttt ttcacgtttt gatcagcaat ccattgtgca
    11341 ttttcgccaa gcaattgtat gctattgttg tatcgtgaat aatcaaggag gggaaaatcg
    11401 ctaccaaaaa gtattttctc ttttccacat attgtaatgg ctatcgaata aatagtatca
    11461 ctataaagaa agggtgatgc agcgctatca tagtagacat gctgcaaaga tttttttacc
    11521 tcaggcatca attcataaaa tagtagccct ccaccccaat gtgaacagat gaccgttgtg
    11581 tttgtatact gtgacaggag tgcatataag cgctgcatat cggtagtata cttaccggta
    11641 tagatatgtc caacaggttc attgacatgc aggcatacag gtaattccat atcccctgct
    11701 attgcaagga tgttacttac tatggtttcc tcttcttcag taaatccatt aacataaaaa
    11761 gcaacctcgc caataccata taatcccatc tgtttaatat tttttatttg ctgatacata
    11821 tcagtatcca gagctatcgc cccaaatgca tatactttac ccttatgtcg ttcctgtacc
    11881 atagccatat attcattgtg gcgctgtgca tagtcatttc tttgccatgt aaatcccagc
    11941 gcaactgatg catcaacacc attgttttgc atgctatcaa tcaatagggc atcattggca
    12001 atttttgctt tcccatagta gagcaacgca aactgtttat catcacagta gcgatcttta
    12061 tgcacaatca tatcatcagt aaagatatga gcatgactat ctataatcat tgtgctatcc
    12121 tataatgtat tgacataaaa caggtcatct gtttaaaatt aactgcattt ccatgctatg
    12181 aacaatgact atgtacaaca tagaatattt tttggaacaa gcaaaagaaa atttccatac
    12241 actgcgtgtt atcggtgaag atggcaatac taagtatata cacagtaaat attatcctac
    12301 cagagaagca tcatatttaa aggatattgt caatcctgaa aaatatgatg ttttaataat
    12361 tttaggtatt gggctggggt accatttagc atatttaact gagataatcc atagctttac
    12421 ccacattata tgcattgata taaacaacta ccttacaaat gcctgtatac ctgaacatat
    12481 acatttgctg ctacaaaatc ctgcaattga aaccatcatc attgatccat tgatgcctga
    12541 gcagctatac aaactgcttt caatcaattg cagcaaaggg atacaggtta ttgaccacca
    12601 ggcgtcgttt gctgcattcc catctgcttt tgcatccatt aaaaatatag tccagcaaat
    12661 catacaaaaa tccctatcca atcaactgac aacaaactat tttgcagctc gctattgtaa
    12721 aaacgctatc atgaatatac ccccattgcc cacatgttac ccggtgatac agtgtaacaa
    12781 catgtatcac aatattccct gtatcattgt tgctccagga ccatccttag aggaaagcct
    12841 gcctttatta tataaaaatc aaaacaaggt atatatcatt agcgtggatt cagccgttaa
    12901 agtgttatca tcctatagta ttgaaccaga ttatattatt acgatagacc cccaaccaat
    12961 cgtttatgct cacctttttg gggtacacca ccacgctatc attgttgcaa tgctgacggc
    13021 tcatcctgcc gttttttcat cgccggttat tgtatcactg aacagtcatc cattgtgtca
    13081 gcttattgaa caggttttcc ctgcaaccat tggttcaatc gatagtagaa ctggcactgt
    13141 ccttggtgac gctgtatcct ttgcgcaatt ttctggattt tctcctattg gcatagcggg
    13201 tgctgatttt tcattccctc atttcataac ctatgtacgc tccacagaat atcactatcg
    13261 ctatgcagca gctcataccc ggttatcccc aattgaaaca agcaatatgc agtatatctt
    13321 ccaggcatca aaaggtacgc ttgttgagca gcgatattca aggaaggtat ttatccagta
    13381 tcgcaacaca atcgatgcac ttatctacaa taccattacc taccagatca atgtttcagg
    13441 gcttcaattg aaaaatgctg taccactatc tacagaagat ttctttacct tgccagcaat
    13501 catgcactat cataaaaaac tatcgccgca ggctttgaaa cagcccatgg atattcattg
    13561 ggagcggcta tattcatatt tatacaatga tagcattttt aatgagctga tgaaagcttc
    13621 taaggtaggc gatagttatg cttcgcaatt aaaactatta ttgaaacttt tactacaaaa
    13681 gaggtaatac tatgcggatt ggggttactg gtatctacgc ttcagggaaa ggcactgttt
    13741 gctccatgtt tgaacagtta ggagcgacag ttattgatac tgatatcatt gcacgagaaa
    13801 ttgttacccc gccaagccct gttctggatt ctttagttgc tacatttggc agggacattc
    13861 tcaaccctga tggaacgctc aatcgacgct atgtagccaa tatggtattt aacgatgcga
    13921 acaaggtcaa tgcactcaat gctattactc atccagcaat actctgtgaa gtactgcaaa
    13981 gaacatccaa tcattcaacc atctacatgg tcaatacccc gctccttttt gaaactgaat
    14041 tttataccta tatggataaa actatagtgg ttatagccaa tcaacaacag gttgtaaagc
    14101 ggggacagat gcgtgatggt attactgaaa gagagatcaa cgagcgattg cagcatcaaa
    14161 tttcacttaa tgaaaaaatg aaattagccg attatatcat agataattcc aaatcattag
    14221 aaaatactaa aaagcaggtt gaagaattat ggaaaatttt gatttcaatg aaaaaccagt
    14281 agcaaaggaa aaaaatgttt tccttctgca tctggatatc ccgcgaataa tcttgatttg
    14341 tgctgttatc attggtattg ttgttactac ttttctatta ggtatgaact ttagcgaaaa
    14401 caaagtcgtt tctaacactg cactggtatc tcctgatata cccaatgata cacttgcaca
    14461 gcttgataaa ggaatcgatt taggaacttc accacaaaat cccgattttt caacacttga
    14521 taatgctcct gacagcctca ccgatactct gcccagggtt gaagaaccaa aaacatacac
    14581 agaaaacgtc actaataaag aagctaaccc aggcttttcg ttagccaaca atgatattct
    14641 ttcaactcag ataattgaac aaaaagaaat gaagaaaacc acacctgtta aaaccacatc
    14701 caaaaaaaat accgctacaa caaaaaagac cgtaaagaaa aagcaaatcg ttgaagtggc
    14761 tcaaactgaa gaaattccta gtaaaaacaa agcacaccgt tcatggtcta ttcaggtagc
    14821 atcatataac acaatattca aagctcaaaa agaagcaaaa tatttacact caatgaacta
    14881 tgatgccttt gttgataaag cagatatttc gggtaaaaca tactatcgcg taaaaattgg
    14941 accgattcta aaagaagata aagcaatagg tatgttacag gaaattcaat caatcgataa
    15001 gtacaaagaa agctatattg taaaagaata attttgttat cctgctactc cctgcagcag
    15061 gataactatt attgttacta atttctttgt attattcttt aacttcatat aaaacagcaa
    15121 aatccgttac ttcatcatca ccatctaa
//
LOCUS       DAOOJH010000055        14935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3855_length_14935_cov_8.915591, whole genome shotgun sequence.
ACCESSION   DAOOJH010000055 DAOOJH010000000
VERSION     DAOOJH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3855_length_14935_cov_8.915591"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1451
                     /locus_tag="PLJ75_08145"
     CDS             <1..1451
                     /locus_tag="PLJ75_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HPD05222.1"
                     /translation="IPLPNLEFKFVAANTLIGLEIFPTIMNLRSNNDGIDINLDKLIE
                     ELRKLRKEYFNSIENKLELQKKFVELQKQIFERSLNWQKDIGQQIQLSMWEPFKFYSS
                     NWFDPEWMFGVTDGFDIVIGNPPYGIKMDNIDNLIKTYKHFDRQKNSASFFIELGSLL
                     SNSKGIIAYIIPKSLSFSEGWKKTRELVTHKNRLISVIDVSKAFEDVKLEQIIICFSK
                     KTIIGKYQFNVGERWSGEIKVSDKISKSMIDQLDILPIYINKRKQSILSKLQQGSILL
                     SEISKTFRGLPFQSKLSNSGQPILRGKNIGKYKIYGSIDKISLPQEILDSAKVIEILK
                     PKIISQNIVTHVMNPYDRIMVIATIDTEGFLTLDTVMNTYLTTDRFTLQYVLAILNSQ
                     LASWFYCWFVYNKAIMTMHFDKYYMGKLPIKDVDLKTQQCLINLIDQILTAKKQNPSA
                     DTTRWEREIDTLVYKLYDLTDEEIQVVEGNVT"
     gene            1476..2036
                     /locus_tag="PLJ75_08150"
     CDS             1476..2036
                     /locus_tag="PLJ75_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05223.1"
                     /translation="MNPHNHHRRSIRLRGYDYTQPGAYFITMCTHNRECLFGDIANGE
                     MVLNEWGKMVWDEWFKSQSIRNEITLYDNEFAVMPNHIHGIVWIVNTDNIGVNDVGAN
                     GRSPLQLPMTQPIGSPKRSLSSFVAGFKSAVTKQINQLRQTPGMPVWQRNYYEHIIRN
                     NNELHRIQQYIINNPKNWELDENYHI"
     gene            2017..2580
                     /locus_tag="PLJ75_08155"
     CDS             2017..2580
                     /locus_tag="PLJ75_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05224.1"
                     /translation="MKIITYNHRRSIRLPGYDYTQPGAYFITICTHNRQCLFGDIANG
                     EMVLNEWGKMVWDEWFKSQSIRNEITLYDNEFAVMPNHIHGIVWIVNTDNIGVNDVGA
                     NGRSPLQLPMTQPIGSPKRSLSSFVAGFKSAVTKQINQLRQTPGMPVWQRNYYEHIIR
                     NNNELHRIQQYIINNPKNWELDENYHI"
     gene            2901..3986
                     /locus_tag="PLJ75_08160"
     CDS             2901..3986
                     /locus_tag="PLJ75_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05225.1"
                     /translation="MNTGMAHNIFSIIIPMLKAGTVAVTIALIMLFISKKLILVRQII
                     CIKRYAAKYQLAMGSITSRFAVNLPVVAESLQIPDEIIDEAVQNSKPSSDSWLYMPHQ
                     GHVYRIHIARDIEEIELENIGALQVHHIGDSRCKGEYAVLTQNGVTIYQCADDHLVLK
                     KHYPHDNIQQFYADSEIVITAAYKTIIIHNRKDTTTLRYSVDEGVCTYVSAKSLVVDS
                     VNDNKKYAVIYFGNTIYFLDLSARYIQFEPIRYITTNVPITGEYEKGFIMNEGFTYGN
                     VFIDIGTYPQKLQYAVFGGRNCVFFIAHEILYCIEVIGQDTLTGLGINIVSHQVDDFC
                     VADNVLVAHQIDNGFIRYFNYEEAEVA"
     gene            4039..4320
                     /locus_tag="PLJ75_08165"
     CDS             4039..4320
                     /locus_tag="PLJ75_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05226.1"
                     /translation="MKVKLKFEDILVVIAYAQVLSMFYFIEDVNTTFIATIIITSWFV
                     AQKVKSHIRKKDVLYTIMHRAHSIKKIVSVDLHRLDGIYEAFKEDELGR"
     gene            4324..4740
                     /locus_tag="PLJ75_08170"
     CDS             4324..4740
                     /locus_tag="PLJ75_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05227.1"
                     /translation="MPIEVKILGYVSFQKLKKPEHALIKYVPINSVIPIFNWQLYNKD
                     LNNQHTPYLLHRKKIHYRYYSPSKRFIILFGKILVIYMCTNVVLNTAKYLQKINNTPP
                     RNHFFENYINTDQRYNNNILKQLKQFKKKNGGVYGK"
     gene            4730..5629
                     /locus_tag="PLJ75_08175"
     CDS             4730..5629
                     /locus_tag="PLJ75_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05228.1"
                     /translation="MVNDQIYYIYDNAVWCRSNNIESGYGYRLEGVAVPIDLYVYNHL
                     SKQKVYLLYNWGVLEYTEKRIYNIKNPVAVVRNHNTLYIATKDAIMVEIKPERFQLVY
                     EKGLSFGTITDIYVDPYFDDNRIETYEHQYNELEILADGKRIFLYNNDYIIEHYDSVS
                     FVQYGKNDRGNKTNEVLTVNDGLVFNSVYYKLNEPAYYPFHVYIKFDFNLQGVYFIAH
                     ENLYLLITNIYTNECAVALMAQGVAKVDGNRDYLVIFGTDNSVGFLKMDEVEAMIQKY
                     NFFPQIEWRTKLDLMIERSRSRL"
     gene            5766..6401
                     /locus_tag="PLJ75_08180"
     CDS             5766..6401
                     /locus_tag="PLJ75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05229.1"
                     /translation="MPRSARNTEEVDSVKQQILDAAVKIITNNGFSKLSMRRIAAQLG
                     VSATTIYNYYTSKDELYFYIRIKGFERLFEYFEKAYAIPVSTREKFIGIIHGYVDFGL
                     TYPDYYEIMFLNRNVPKFLDCVGTPLEAVATKEKAVALKPFIFTVEKLQHHLDIEPKL
                     AYELAMQLWVELNGLVSLCISRLLREVNEKQQRFVDSYLDTIVCRYLPVDM"
     gene            6491..9475
                     /locus_tag="PLJ75_08185"
     CDS             6491..9475
                     /locus_tag="PLJ75_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPD05230.1"
                     /translation="MLLYTTNIRSSKGTRIIIFLFLKFIAFNFNNRKSLNRYLTSKDG
                     WLNFSIGLKTEDNSLQYGISFFEGKVAVTKTIAHDAKAVMIFKNDEAARKLLLGTPTD
                     VIYMLLKSDVRIEGSISYANIFSFFISLLFHDVQKRAMKTERKKDDKTVKRQQPEHNK
                     QLSDEIVKRRKERLKAEGADPHVVYLDDAYLSKYSMDDFPRLQRFLHDHLTKKPAVCA
                     ERAYLLTKWYRENGFEKDSSGNNWDPIVRNALAYKYMMENRKPLINDESLLAGTTTSK
                     PIGCPIYPDGSGIMIWNELMTISYRTYNPFDIDEQTREVLHFDVFPFWLHRNFREWVR
                     DKYNNPLSQQLDERYALYFNWKQATISHTIPNFPKVLAMGTLGMIQEMHEELNSGCGE
                     EKQNCLTAMILCLEGVNAYAQNLSKQAMGDSNREQNPQRKQELLTIADICSRIPMHGA
                     QTLHEAVMAVWIIWIALHMESMNAGLSLGRLDQWLQPYFVNDMKNIPPEKRDEYMKFA
                     LELIGDFYMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPEGTDAVCDMTYIFLKV
                     TEMLSVRDPNVNARYMPGVNSDTYLKRLCEVNLITAATPSMHNDAAIIKALTPMGYPI
                     NDIRDWSATGCVEPTLSHKHFGHTNMQMMNMVAALEMTLNNGYHPLTRLDVGPKTGRP
                     EDGVFTTFDTFFDAFTQQFAFLIDQSIDYNNKLAEAHQLIRPTPLLSAFIEGCLEKGK
                     DVTMGGAKYNSSGTAIIGLADVTDSMMAIKKLVYDEKRYAFADIKKAVDSNFAHDAVL
                     LSLVHKKIPLFGSGSDEAVAMANRITTWAHDHYSKMPHYRGGAYATGFWSMSNHVAFG
                     TLTGALPSGRLAGKPFTPGLTPQPFASKNLLDNIRDVARLNPENCNNNIAFNVKVVPS
                     AIESRQKTVNDMFSYVKTYFELGGMQMQMNVVTSAVLRDAMAHPENYRNLIVRISGYN
                     AYFVTLNRQMQLELIERAEYGI"
     gene            9472..10380
                     /locus_tag="PLJ75_08190"
     CDS             9472..10380
                     /locus_tag="PLJ75_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPD05231.1"
                     /translation="MNKALILDIKGNSLDDGPGIRTVVFFKGCPLNCVWCHNPESKRA
                     TPELSWDGRECIGCYSCKNVCALNAIIPGSTHFINRAVCNLCFACVDVCPSGAMSKVG
                     KLMAVEDIIQTIIKDKPFFDTSGGGVTLSGGEPTVYMQFASELLKALKKIKIHTLIET
                     GGYFNGDEFEANIVPYCDMVYYDIKLIEDFKHKKYCGVSNAKILQNFKKLVELSQKNA
                     IQVMPRIPLVPGITATKHNIEGIAHFLRECGVNRVQLLRYNPLWPEKCFKIGSNGNID
                     KALTQWMSQKDYEFYAKILVKNTIQL"
     gene            complement(10446..11429)
                     /locus_tag="PLJ75_08195"
     CDS             complement(10446..11429)
                     /locus_tag="PLJ75_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPD05232.1"
                     /translation="MAQLPSICFFGCGYIAKRHAKLLRKLYPDITLGFSSLYQQDASE
                     YARMFNGTAFKSYEEAATSDYDIAFITTPHAYHKDLAILAANNKKHVIVEKPAARNLK
                     EFDAIIKAVTKNNVRFTVAENYYFKPFIKTIHRHIAKGSIGTPLYVRLTKHNHDIISG
                     WRTDARLMGGGALLEGGCHWVNELVSLSNATPEEVLAVKPQMQYPTSIPFEDTIHITV
                     KFNNGSFATLFHSWYVPNTLKGVALSKIYGTEGTVTFESNGLFTVVKGKRFAMNFSFR
                     DFLGFRAMHQSFIENYRTQKEWEPNWKRIRMELSLIEAAYRSLKTNKFESI"
     gene            complement(11434..12999)
                     /locus_tag="PLJ75_08200"
     CDS             complement(11434..12999)
                     /locus_tag="PLJ75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPD05233.1"
                     /translation="MKTIKSSYEYVIIGSGFGGSFAAYALAKAGKQVLVIERGVWAKR
                     DDTCWDEYALHLQQKPLYRGHTPYAVDQHGTLEYDWPDDTVGGMSTFYGAAAFRMRNE
                     DFEGPPAPDSDTRNAELAWPFRYDTLAPYYDKAEELQCVAGIQGQDITQPPRTKDYPQ
                     QPPLNLSRPSQKVWDAATSLGLHPFYIPMAINFSGVCNNHTCILCNTCDHYLCKIQAK
                     NDLSVSVLPRAMHYGAQVLDNHRAIKINFVKSKAQSVDVINQQTRQVMTIQCKYLIVA
                     SGPIATPHLLLASGITGYPNTNHIGHNLMRHINGVVSGVVPYIANPDNTLAKRIAISD
                     FYFGDSSGKMLPEGNWGIIQDIATPGKGVIKANAPFGLKNIAATFSQFLINQLVIAED
                     IPQYTNRIYCSDPRDIFGMPQVMIYHRYHSRDIQARNALYKQAKRILRKAGALFFYTM
                     PIVTFSHALGTCRMGNNKNLSAINPECRLWDIENCYITDASALPTGGSVNPSLTIAAN
                     ALRVTDIIVQSRG"
     gene            complement(13028..13528)
                     /locus_tag="PLJ75_08205"
     CDS             complement(13028..13528)
                     /locus_tag="PLJ75_08205"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HPD05234.1"
                     /translation="MQLGHVAVSFIIASYAPQITGGQMQAFSLESILVAFAAHWLPNL
                     DVIPIKLGWAKDSFHCTWSHSLVFAIAVFLLLLPFNSAWAMLSLVSLIVHYLADMPSS
                     VGLPLLMPFTKKRFTINLWADTGHSGWDALIGTYVQAWTWILEGGAFVFLFVRMYQLS
                     IWPFIS"
     gene            complement(13688..14380)
                     /locus_tag="PLJ75_08210"
     CDS             complement(13688..14380)
                     /locus_tag="PLJ75_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPD05235.1"
                     /translation="MDGTLIRTHGAGKKAMVQAAITVYGTAGDMDTVDFQGKTDPLIL
                     IQSLTPHGINQNTIFERMEDYKTLYFDLLQQTMPQSDAVLLPGIRELLHTLKKYDNLI
                     VGLLTGNFYKGAMIKLNYFDIFHYFTMGVFADDTYIRNEMPFVALQRLQAVTGTVFTT
                     EDLIIIGDTIYDIECGKTSGATTVAVATGWTDAETLLQHNPDYFFHELSDTNNFLYSI
                     KELQDCIQLHTQ"
     gene            complement(14398..14628)
                     /locus_tag="PLJ75_08215"
     CDS             complement(14398..14628)
                     /locus_tag="PLJ75_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05236.1"
                     /translation="MLPRTTQLSILFAMIIAIIIVLLFEIPARPFTIRLAIWLYVLSL
                     LPGLGIAMLFDPAPREARERFLKINFYQEKKS"
BASE COUNT         4782 a         2674 c         3061 g         4418 t
ORIGIN      
        1 taataccatt gccaaatctt gagtttaaat ttgtagcggc aaatacgttg attgggttag
       61 aaatattccc tactataatg aatttaagaa gtaataatga tggaattgat ataaatttag
      121 ataaattaat cgaagaatta cgaaagctac gtaaagaata ttttaactcc atagaaaata
      181 aacttgaatt acaaaagaaa tttgttgaac tacagaaaca aatatttgaa agaagcttaa
      241 actggcaaaa agatattggt caacaaattc aattatcaat gtgggagcct tttaaatttt
      301 attcaagcaa ctggtttgac cccgaatgga tgtttggcgt aaccgatggc tttgatatag
      361 ttattggaaa tccgccgtat ggcattaaaa tggataatat tgataatctt ataaaaacct
      421 ataaacattt tgacaggcaa aaaaattccg cttcattttt tatagaactt ggtagtttat
      481 tatccaactc taaaggaata attgcttata ttatacctaa atccttgtct ttttctgaag
      541 gctggaaaaa aacaagagaa ctagttacgc ataaaaatag attgatatca gttattgatg
      601 tatctaaagc atttgaagat gtaaaattag aacagataat tatttgcttt tcaaaaaaaa
      661 ctataattgg taaatatcaa tttaatgtcg gtgagaggtg gagtggtgaa ataaaagtaa
      721 gtgataaaat cagtaaaagt atgatagatc aacttgatat tttgccaata tatattaaca
      781 aaagaaaaca aagtatttta agtaaattgc aacaggggag tatattactt tctgaaatat
      841 caaaaacatt cagaggatta ccattccaaa gtaagttgtc aaactcaggc cagccaattt
      901 taagaggcaa aaacataggc aaatataaaa tctatgggag cattgataaa atttccttac
      961 ctcaagaaat attagatagt gcaaaagtga tagagatttt aaaacctaaa atcatctctc
     1021 aaaatattgt tactcatgtt atgaatccat atgataggat aatggttata gcaacgattg
     1081 atacagaagg atttttaacc cttgatacag tgatgaatac atatttaaca acagatagat
     1141 ttacgctaca atatgtgctt gcaatactta attcacaact tgcctcttgg ttttattgtt
     1201 ggtttgttta taacaaagcg ataatgacaa tgcattttga taaatattat atgggaaaac
     1261 ttcccataaa agatgttgat ttaaaaaccc agcagtgttt aatcaatctt attgaccaaa
     1321 tcctcaccgc caaaaaacaa aacccaagcg ccgatacaac ccggtgggaa cgggaaatag
     1381 atacactggt ttacaagttg tatgatttaa cggatgaaga gatacaggtg gtggaaggaa
     1441 atgtaacata aaaaaatata ataacaaata caattatgaa tccgcacaat caccaccgcc
     1501 gttcaatccg tttgcgggga tacgattaca cccaacccgg tgcatatttt ataaccatgt
     1561 gcacccacaa ccgggaatgt ttatttggtg acattgcaaa tggcgaaatg gtgttaaatg
     1621 aatgggggaa aatggtatgg gatgaatggt ttaaatccca atcaatacga aatgaaatta
     1681 cattatatga taatgaattt gccgtaatgc ccaatcatat ccacgggatt gtgtggattg
     1741 taaataccga caatattggg gtgaatgatg taggggcgaa cggccgttcg cccctacaat
     1801 tgccgatgac gcaacccatt ggatccccaa aacgttcatt gtcgtcattt gttgcggggt
     1861 ttaaatccgc cgtaaccaaa caaatcaatc aattgcgcca aacacccggt atgcccgtct
     1921 ggcaacgcaa ttattatgaa cacatcatcc gtaacaacaa tgaattacat cgcatccaac
     1981 aatatattat aaacaaccct aaaaattggg aactggatga aaattatcac atataatcac
     2041 cgccgttcca tccgtttacc gggatacgat tacacccaac ccggtgcata tttcattacc
     2101 atttgtaccc acaaccgcca atgtttattt ggtgacattg caaatggcga aatggtgtta
     2161 aatgaatggg ggaaaatggt atgggatgaa tggtttaaat cccaatcaat acgaaatgaa
     2221 attacattat atgataatga atttgccgta atgcccaatc atatccacgg gattgtgtgg
     2281 attgtaaata ccgacaatat tggggtgaat gatgtagggg cgaacggccg ttcgccccta
     2341 caattgccga tgacgcaacc cattggatcc ccaaaacgtt cattgtcgtc atttgttgcg
     2401 gggtttaaat ccgccgtaac caaacaaatc aatcaattgc gccaaacacc cggtatgccc
     2461 gtctggcaac gcaattatta tgaacacatc atccgtaaca acaatgaatt acatcgcatc
     2521 caacaatata ttataaacaa ccctaaaaat tgggaactgg atgaaaatta tcacatataa
     2581 tcaccgccgt tccatccgtt taccgggatg tgattataca caacccggtg catattttat
     2641 aaccatttgt accctcaacc gggaatgttt gtttggtgac attgcaaatg gcgaaatggt
     2701 gttaaatgaa atggggaaaa ttgcacacca atattggttg gatattctca atagtacgca
     2761 taaaaaaata tcgcaataaa aaataaaaaa aatttcacca gggacattat atgtcattca
     2821 ccaggaagta tattccctat ataatgaagg aaaaaatagt ctaatataca caataaagtg
     2881 aatatgaaaa agggggctat atgaacactg gtatggcaca taatattttt tcaataataa
     2941 ttcccatgtt aaaggcaggt acagtagcgg taactatcgc cctgattatg ctttttatta
     3001 gtaaaaagct aattttggtg agacaaataa tttgtataaa acggtatgct gctaaatacc
     3061 agttggcaat gggttctatt accagccggt ttgctgttaa tttgccggtt gtagcggaat
     3121 cgttacagat accagatgaa ataattgatg aggctgtgca aaatagtaag ccatcaagcg
     3181 atagctggct gtatatgccc catcagggac atgtgtatag aatacatatt gcgcgtgata
     3241 ttgaggaaat tgaactggag aatataggcg cgcttcaggt acatcatatt ggcgatagtc
     3301 gctgtaaagg ggaatatgct gtgctaacac aaaacggcgt tactatatac cagtgtgctg
     3361 atgatcattt agtgctaaag aaacattatc ctcatgataa catacaacaa ttttatgcag
     3421 atagtgagat agtaataaca gcagcgtata aaacaataat catacataac aggaaagata
     3481 caactacatt acggtacagt gttgatgaag gagtctgtac gtatgtgtca gcaaaatcgt
     3541 tagttgtcga tagcgtaaat gataacaaaa aatatgcagt aatctatttt ggaaatacca
     3601 tatacttttt ggatttatca gctagatata ttcagtttga accaatccgt tacattacaa
     3661 caaatgtacc tattacaggc gaatatgaaa aaggattcat aatgaacgaa ggatttacct
     3721 acggaaatgt ttttatcgat attgggacat atccacaaaa gctccagtat gctgtttttg
     3781 gtggcaggaa ttgtgtattc ttcatagcgc atgaaattct gtattgcatc gaggttatag
     3841 ggcaggacac attaacaggt cttggcataa atattgtatc ccatcaggtt gatgactttt
     3901 gtgttgctga taatgtttta gttgctcacc agattgataa tggatttatt cgatatttta
     3961 attatgaaga agcagaggta gcataagcac ataatgatta ctatgcaata taggtaagcg
     4021 tacaatacag gagggagcat gaaagtaaaa ctaaaatttg aagatatact ggtcgttatt
     4081 gcctatgcac aggtgttatc aatgttttat ttcattgaag atgttaacac aacttttata
     4141 gcaacaatta ttattacctc atggtttgtt gcacaaaagg taaaaagcca tatacgaaaa
     4201 aaagatgttc tgtacaccat tatgcacaga gctcattcaa ttaaaaagat tgttagcgta
     4261 gatttacaca ggcttgatgg tatttatgaa gcatttaagg aagatgaact aggcaggtaa
     4321 actatgccta ttgaggtaaa aatactggga tatgttagct ttcaaaaatt aaaaaaacct
     4381 gaacatgctt taataaagta tgttccaatt aattctgtta ttcccatatt caactggcag
     4441 ttgtataata aagatttaaa caatcaacat acaccatacc ttttacatcg gaaaaaaata
     4501 cattaccggt attattctcc gtcaaaacgg tttattatac tttttggtaa gattcttgta
     4561 atttacatgt gcacaaatgt tgtgttaaat actgcaaaat atctccagaa gattaataat
     4621 acaccccctc ggaatcattt tttcgaaaat tatataaata ccgatcaacg gtataacaat
     4681 aacattttaa aacaacttaa acaatttaaa aagaaaaacg gtggtgtata tggtaaatga
     4741 tcaaatatat tatatttacg ataatgcagt atggtgtaga agtaataata ttgaaagtgg
     4801 gtatggatac aggctggaag gtgtagctgt tccaattgac ttgtacgtat ataatcactt
     4861 gagtaagcaa aaagtgtatt tactgtacaa ttggggtgtg cttgaatata ccgaaaaacg
     4921 aatatacaat ataaaaaatc ctgttgccgt agtacgtaat cacaacacac tttatattgc
     4981 aacgaaggat gcaatcatgg tagagataaa accggaacga tttcagctgg tatatgagaa
     5041 aggtttatcg tttgggacaa taaccgacat atatgtagat ccatattttg atgacaatag
     5101 gatagaaaca tacgaacatc agtacaatga acttgaaatt ttagctgatg ggaagcgcat
     5161 atttttatat aacaacgatt atataataga acattatgac agcgttagtt ttgtacaata
     5221 tggaaaaaac gatcgtggta acaaaacaaa tgaagtatta acagtcaatg atgggcttgt
     5281 attcaacagc gtgtactata aactcaatga acctgcttat tatccttttc atgtttatat
     5341 caaatttgat tttaatcttc agggtgtgta ctttattgca cacgaaaatt tgtatttgct
     5401 cataactaat atatatacca atgagtgtgc tgttgcattg atggcgcagg gagtagcgaa
     5461 agtggatggt aatagggatt acctggttat ttttgggaca gataattccg ttggtttttt
     5521 aaaaatggat gaagttgaag caatgataca aaaatataat ttttttccgc aaatagaatg
     5581 gagaacaaag ttagatttga tgatagaacg ctctcgatca agactttaag cacatttacc
     5641 cctgtattta ctgtaagttt aatagcctta tttgaaaata actatcgaaa aaattttatt
     5701 tttgcttggc aaataaggtt aacactgtta aaataacact gataatatat tagaggccaa
     5761 aaactatgcc acgaagtgca cgcaacacag aagaagttga tagtgttaaa cagcagatac
     5821 ttgatgctgc cgttaagatt atcactaaca atggattttc aaaactcagc atgcggagga
     5881 ttgcagcaca gttaggggtc agtgctacaa ccatctacaa ctattatacc agtaaggacg
     5941 aactatactt ttatatacgc attaagggct ttgaacggct gtttgaatat tttgaaaaag
     6001 catatgccat accggtttct acccgtgaaa agtttatagg tattatacat ggctatgttg
     6061 attttgggtt aacctatcct gattattatg aaataatgtt tttaaaccga aatgtgccaa
     6121 agtttctgga ttgtgtgggt acgccacttg aagctgttgc aactaaagaa aaagcagtgg
     6181 cattgaagcc tttcatattt acagtagaaa agttacagca tcatctggac attgaaccaa
     6241 agctggcgta tgaattggca atgcaactat gggtggagtt aaacggactg gtaagtttgt
     6301 gcatcagcag gctcctcaga gaagtaaatg aaaagcagca gcgttttgtc gatagttatc
     6361 tggataccat tgtatgccgt tatttgccgg ttgatatgta aatgcagtgt taggaataac
     6421 tcccccttgc cttgcgaagg agaggggttg ggggtgaggt tatactttag tcattatcag
     6481 gaggtttgcc atgttgctgt atacaacaaa tatcagatca agcaagggta ccagaataat
     6541 aattttctta tttttaaaat tcattgcttt caattttaat aatcgtaaat ccctcaaccg
     6601 ctatcttaca tctaaagacg gatggcttaa ttttagcatt ggtctgaaaa ccgaagataa
     6661 ttctttacag tatgggattt ctttttttga ggggaaagtt gctgttacaa aaactatcgc
     6721 ccatgatgcg aaagctgtta tgatttttaa aaatgatgaa gcggcacgca agctgctctt
     6781 aggtacgcca accgatgtca tctacatgct attaaaaagc gatgtgcgaa tagaaggaag
     6841 cataagctat gcaaatattt tttcgttttt catttcattg ctgtttcatg atgtacaaaa
     6901 acgtgcaatg aaaactgagc gcaaaaaaga tgataaaaca gttaaacggc aacaaccaga
     6961 gcacaataaa cagttaagcg atgaaatcgt aaagcgtaga aaggagcgtc tgaaagctga
     7021 gggagccgac ccgcacgttg tgtatcttga tgatgcctat ttgtctaagt atagcatgga
     7081 tgacttcccg cgtttacaac gatttctaca tgatcatctg acaaaaaaac cagcagtgtg
     7141 cgctgagcgt gcatatcttt taacaaaatg gtaccgggaa aatggatttg agaaggattc
     7201 atccggtaac aattgggacc ccattgtgcg caacgcattg gcgtacaaat acatgatgga
     7261 aaaccgaaag ccgcttataa acgatgagtc gcttttagcg ggaactacca ccagcaagcc
     7321 cattggctgc cccatatatc ctgatggaag cggcattatg atatggaacg aactcatgac
     7381 gatatcatac cgtacctaca atccgtttga tattgatgaa caaacacgtg aagttttaca
     7441 ctttgatgtt tttcctttct ggctgcaccg caatttcaga gagtgggtgc gcgacaaata
     7501 taataatccg ttgagtcagc agttagatga acgatatgcg ctgtatttca actggaagca
     7561 ggcaaccata tcacatacta ttccaaactt tccaaaagtt ttggctatgg gtactctggg
     7621 aatgatacag gaaatgcatg aagagctaaa tagcgggtgt ggtgaggaaa aacaaaattg
     7681 tttaacggca atgattttgt gtctggaagg tgtcaatgcg tatgcacaaa acctttcaaa
     7741 acaggctatg ggcgatagta accgggaaca gaatcctcag cgtaaacaag aacttttgac
     7801 aatagcggat atttgcagtc ggatacccat gcatggagca caaacgctgc atgaagcagt
     7861 tatggcagta tggattatat ggattgcact gcacatggag agtatgaatg cagggctttc
     7921 attgggtagg cttgatcaat ggctgcaacc gtacttcgta aacgatatga aaaacatccc
     7981 gcctgaaaag cgtgatgaat acatgaaatt tgcccttgaa cttattggtg atttttacat
     8041 gcgctgtacc gaccattttc cgcttacccc cgatttagct aacttctact ttggcggcag
     8101 ttcttccgac caggctatta cactgggcgg agtaacgcct gagggcaccg atgcagtatg
     8161 cgatatgacc tacatctttt taaaggtaac tgaaatgctc tcagttcgtg atcccaacgt
     8221 caacgcgcgc tacatgccgg gtgttaacag tgacacctat ttaaagcgtt tatgcgaggt
     8281 aaatttaata accgctgcta caccatcaat gcacaacgac gctgctatca tcaaggctct
     8341 gacccccatg ggatatccta ttaacgatat acgagactgg tcagcaaccg gctgtgttga
     8401 accaacacta tcgcacaaac actttggcca taccaatatg cagatgatga atatggtagc
     8461 agcactggag atgacactga ataatggcta tcatccgctt acaaggcttg acgttggacc
     8521 aaaaacgggc aggccggaag atggagtatt tacaacgttt gatacgttct ttgatgcgtt
     8581 tacacaacaa tttgcgtttt taattgatca atctatcgac tacaataata agttggcaga
     8641 agcccaccaa ttaatacgcc cgacaccgct tttatcagca tttattgagg gatgtcttga
     8701 gaagggtaag gatgttacca tgggcggtgc aaaatacaat agctcgggta cggcaattat
     8761 agggctggct gatgttaccg attccatgat ggcaattaaa aagctggtat atgatgaaaa
     8821 acgatacgcg tttgctgata taaaaaaagc tgttgatagt aattttgcac atgatgctgt
     8881 actgctttcg ctggtgcata aaaaaattcc tctttttggc tcgggcagtg atgaagccgt
     8941 ggctatggct aacagaatta ctacatgggc gcatgatcat tattctaaaa tgcctcatta
     9001 ccgtggagga gcatatgcaa ccggattctg gtccatgtca aatcacgttg cctttggtac
     9061 gcttaccggt gcgctgccgt cgggaagatt ggcgggaaag ccgtttacac caggattaac
     9121 tcctcagccg tttgcttcaa aaaatctgct tgataatatt cgggatgttg cacggctcaa
     9181 tcctgaaaat tgtaacaaca atattgcgtt taacgtgaag gttgtgccct ctgcaattga
     9241 atcgcgccag aagacagtca atgacatgtt ttcatacgtg aaaacctatt ttgaattagg
     9301 cggcatgcag atgcagatga atgttgttac atcggctgtg ctgcgcgatg caatggctca
     9361 tcccgagaac tatcgcaacc tgattgtacg tatttcagga tacaatgcct attttgtgac
     9421 attgaaccgt cagatgcagc ttgaacttat tgaaagggct gaatatggta tatgaacaaa
     9481 gcgctgattc tcgatatcaa aggaaattct ctggacgacg gtccgggcat tcgaaccgtc
     9541 gtctttttta agggatgtcc gctcaactgc gtatggtgtc ataatcccga aagcaagcgg
     9601 gcaacgccag agctttcctg ggacggcagg gaatgcattg ggtgttacag ttgcaaaaac
     9661 gtgtgtgcgc taaatgcaat aattcccgga agtacgcatt ttattaatcg tgctgtatgc
     9721 aatctttgct ttgcctgtgt tgatgtatgc ccttcaggtg caatgagtaa agttggcaaa
     9781 ctaatggctg tagaagatat tatacagact attataaagg ataagccttt ttttgataca
     9841 tccggcggcg gggttacgct atcgggtggc gaaccaacag tatatatgca atttgcatca
     9901 gaattgttga aagcattgaa gaaaataaaa atccatacac tcattgaaac aggcggttat
     9961 ttcaacgggg atgaatttga agccaatata gtaccatatt gtgatatggt gtattatgat
    10021 atcaagctta ttgaagattt taagcataaa aaatattgtg gggtttcaaa tgcaaaaatt
    10081 ttgcaaaatt ttaaaaagct tgttgaactg tcacaaaaaa atgccattca ggtaatgcca
    10141 cgtataccac ttgttccggg aataacggca acaaagcata atatagaagg tattgcgcat
    10201 tttttacgcg aatgcggtgt taaccgtgtc caactgctac ggtataatcc attatggccc
    10261 gaaaaatgct ttaagatagg gagtaatggc aatattgaca aagcattaac acaatggatg
    10321 tcgcaaaaag actatgagtt ctatgcaaaa atattagtga aaaatactat acaattatag
    10381 tatgggataa tagaaaatca taacacatac atgatagttt tttcataaag atgtgcagag
    10441 tgctgttata tactttcaaa tttgttagtt tttaacgaac ggtatgcagc ttcaatcaat
    10501 gacaattcca tgcgtatgcg tttccagttt ggttcccatt ctttttgtgt gcgatagttc
    10561 tcgataaatg attggtgcat ggcgcgaaag cctaaaaaat cgcggaaaga aaaattcatt
    10621 gcaaagcgct tccccttcac aacagtgaag agtccgttgc tttcaaaggt gacggtgcct
    10681 tccgtaccat atatttttga aagagcaaca cctttcaatg tattggggac ataccacgaa
    10741 tgaaataatg tagcaaaact accgttgtta aatttaacag tgatgtggat ggtatcttca
    10801 aagggtatac tggttggata ctgcatttgt ggttttactg ccagtacttc ttctggtgtt
    10861 gcgttgctca gtgatacaag ctcattaacc cagtgacagc cgccttctaa gagtgcgccg
    10921 ccacccataa gccgggcatc ggtgcgccat cccgagatta tgtcatggtt atgcttggtt
    10981 aatcgcacat acagtggtgt gccaatggag ccttttgcaa tgtgcctgtg tattgttttt
    11041 atgaatggct tgaagtaata attttcggca actgtaaatc ggacgttatt tttggttaca
    11101 gctttgatta ttgcatcaaa ttctttgagg tttcgtgctg ctggcttttc tactataaca
    11161 tgttttttgt tgtttgcagc taatattgct aaatccttat gatacgcatg aggtgttgta
    11221 ataaacgcaa tgtcataatc cgatgtggct gcctcttcat agcttttaaa tgctgttccg
    11281 ttaaacatgc gtgcatattc tgatgcatcc tgttgataca ggcttgaaaa gcccagtgta
    11341 atatcggggt atagtttgcg caatagttta gcatgacgct ttgcaatata tccgcatcca
    11401 aagaagcaga ttgatggaag ctgtgccatg atgttatccc cttgattgta caataatatc
    11461 tgtcactcgc agtgcatttg cggctattgt taagctggga ttaacagaac cccccgttgg
    11521 cagggcgctt gcatcggtta tgtagcagtt ttcaatatcc cataatctgc attctggatt
    11581 aatggctgaa aggtttttat tgtttcccat tctgcatgtt cccaatgcgt gggaaaaagt
    11641 aacaataggc atggtataaa aaaacagtgc acctgctttg cgtaatatac gttttgcctg
    11701 tttgtataat gcattgcgtg cctgaatatc gcgactatga tagcgatggt atatcattac
    11761 ctgaggcatg ccaaatatat ctctggggtc agagcaatag attctgttgg tatactgtgg
    11821 aatatcttca gcaatgacaa gctggtttat aagaaattgt gaaaatgttg ctgcaatatt
    11881 ttttaatcca aacggtgcat tggcttttat cacgcctttg ccgggggtgg ctatatcctg
    11941 tattatgccc cagttacctt ctggcaacat tttaccggaa ctatcgccaa aataaaaatc
    12001 agaaattgcg atgcgctttg ccagtgtatt atcaggattg gcaatgtagg ggacaacacc
    12061 gctcacgacg ccgttgatat gacgcataag gttgtgtcca atatggtttg tgtttggata
    12121 accggtaatt ccggatgcca gcaataaatg gggtgttgca atgggccccg aagctactat
    12181 caaatactta cactgtatgg tcatgacctg gcgtgtttgc tgattgataa catcaaccga
    12241 ttgagcttta ctttttacaa agttgatttt tattgcccgg tgattatcca gtacttgagc
    12301 gccatagtgc attgctcggg gcagaacgct taccgagagg tcattctttg cctgtatttt
    12361 gcaaagatag tgatcgcagg tgttgcacag tatgcatgta tggttgttgc acacgccgct
    12421 aaaattaatt gccatgggga tataaaaagg atgcagccct aatgaggttg cagcatccca
    12481 caccttttgc gaagggcgtg aaaggtttaa tggtggttgc tgtggatagt cctttgtacg
    12541 gggcggttgt gttatgtcct gtccctgtat gcctgctaca cattgcagtt cttctgcttt
    12601 atcatagtat ggggctaatg tatcgtacct aaatggccat gccagctctg cattgcgcgt
    12661 atcagaatca ggtgctggcg gtccttcaaa gtcttcattg cgcattctga atgctgcggc
    12721 tccatagaag gtactcatgc ctcctactgt gtcatcgggc cagtcatatt ccagtgtgcc
    12781 atgttggtcc actgcatacg gtgtatggcc acgatataac ggtttttgct gcaaatgcag
    12841 tgcatattca tcccagcagg tgtcatcgcg ctttgcccat actccacgct caataacaag
    12901 aacctgtttc ccggctttgg ctaatgcata cgctgcaaat gaacctccaa agccactgcc
    12961 aatgattaca tattcatacg aggattttat tgttttcatt ttatattaat ttacagtaac
    13021 tatcgcccta agaaataaat ggccatatgc tgagctgata catgcgcaca aataaaaaca
    13081 caaatgcgcc accttctaat atccatgtcc atgcctgtac ataggtgcca atgagagcat
    13141 cccatcctga atgaccggta tctgcccaca ggttgatggt aaagcgtttt ttggtaaagg
    13201 gcataagtag cggcagtcct acgctgcttg gcatatcggc tagataatga acaataaggc
    13261 taaccagcga aagcattgcc catgcactgt taaatggcaa aagaagcaaa aaaactgcaa
    13321 tggcaaaaac aagtgagtgc gaccatgtac agtggaatga atcttttgcc cagcccaatt
    13381 taatgggaat aacatccagg ttgggtaacc agtgagcagc aaatgctacc agtatacttt
    13441 ctaatgaaaa tgcctgcatc tggccaccgg taatctgcgg tgcataggat gcaataataa
    13501 atgatacggc aacatgacct aattgcatgt gttcctccag caacaataag atagtagcgt
    13561 caaatgtatt gtgtttaaac gatgcatgtt gcatcaattg tggcatcgtg tgttaacata
    13621 atatcctgac acatagattt ttaatcctta ccatgtttca tgatgacttt acgttgtttc
    13681 caaattgtta ttgagtatgt aactgtatac aatcctgtaa ttctttgatt gaatacaaaa
    13741 aattatttgt gtccgatagc tcatggaaaa aataatcagg gttatgctga agcagggttt
    13801 cagcatcagt ccagcctgtt gcaacagcta ctgttgtagc tcctgatgtt ttaccgcatt
    13861 caatgtcata gatggtatcg ccaatgatta tgagatcctc ggttgtaaaa acagtaccgg
    13921 taacagcctg tagacgttgt agtgcaacaa atggcatttc attgcggata tatgtatcat
    13981 cagcaaaaac gcccatagta aaataatgaa agatatcaaa gtaatttaat tttatcatag
    14041 ctcctttata aaaatttcct gttaaaagtc ctacgatgag gttgtcatac tttttcaaag
    14101 tatgtaacaa ttcccgtata ccgggaagta aaactgcgtc tgactgtggc atggtttgtt
    14161 gtaacaaatc aaagtaaagg gttttataat cttccatgcg ttcaaatatg gtattctggt
    14221 ttataccatg cggtgtcaac gattgtatca gtatcaatgg atcggttttc ccctgaaagt
    14281 ccacggtatc catatcccct gcagtaccat atactgtgat ggcagcctga accattgcct
    14341 ttttcccggc accgtgtgtg cggattagcg ttccatctat atcaaagagt attgctttta
    14401 tgactttttc tcctggtaaa aatttatctt taaaaagcgc tctctggctt cacggggggc
    14461 gggatcaaaa agcatggcaa tccccaatcc cggtaacaat gaaagaacgt aaagccatat
    14521 tgctaaacgt atggtaaatg gacgtgccgg aatttcaaac agtaaaacaa taattatagc
    14581 aatgatcatt gcaaaaagaa tacttaattg tgttgttcgt ggtaacatag tgcaaccctt
    14641 atttgtgata tagtttcaat gtttgtaaca ataaaagttt atagcacata aaatcaataa
    14701 aaaaaatata atagaaaagg ctctgccccc attattttgt tttctccgtg taatcagcaa
    14761 actacatgtt actttgagcg attttttttt taacagtata tgctctgacc cctatactta
    14821 gtgtttttta aaaaaatcac aaaaagtata aaaaaatgct caattctttt taattttttt
    14881 tatctccggc gttatgtata gtagggatgt acattataat aaaaacacgg aggaa
//
LOCUS       DAOOJH010000056        14870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3871_length_14870_cov_10.085251, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000056 DAOOJH010000000
VERSION     DAOOJH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3871_length_14870_cov_10.085251"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..458
                     /locus_tag="PLJ75_08220"
     CDS             285..458
                     /locus_tag="PLJ75_08220"
                     /inference="COORDINATES: protein motif:HMM:NF040425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HPD05237.1"
                     /translation="MLQLVDRIGRLESQLNKNSHNSSKPPSSDGLKKKARKWSQRKRT
                     GKKPAGSLRHLMR"
     gene            531..641
                     /locus_tag="PLJ75_08225"
                     /pseudo
     CDS             531..641
                     /locus_tag="PLJ75_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007349766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(871..1863)
                     /locus_tag="PLJ75_08230"
     CDS             complement(871..1863)
                     /locus_tag="PLJ75_08230"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPD05238.1"
                     /translation="MTELFMRVLLKNLHTPGRHCASTGLRNLAAFHGLGWSEAFCFGI
                     GAGLGIWYIKSNNPPRMIHVRSADIEERFFLHIGMPVTWETYETQQQSHRELIKKLDA
                     GLPAIVQTDLYYLPYYKSSTHFPGHVITVWGYDDDKKVFYITDTERPALIEVAYSDLA
                     QARFCEDNFFKIHGNCIAPAMMKAPVNMQDTIRNAIIYNSRVILDTSISTQGIQGLTT
                     FYNELELWTQFEDWKWAFRFAYQVIERRGTGGGGFRLIYADFLKEATAYLPDIESLQL
                     PYYMQQTAAAWTAFAMALKEASEKEKPEISPVTTCLENVIQAETRYHTKALMLL"
     gene            2081..2854
                     /locus_tag="PLJ75_08235"
     CDS             2081..2854
                     /locus_tag="PLJ75_08235"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD05239.1"
                     /translation="MQSKKMVITVPEKARAYFKKGSDNLGILLLHGFTGTPAEMRYIA
                     EKLHNAGVTVYVPRYPGHGTSLVEMANTSVHHWFTAAREALFELKSHCREVYVAGLSM
                     GGIFAILLAKEFDIKKIALMSVPCTLKEKTIYITPLVGRFKKIIWIPNKTKGVCDERA
                     RKEHICYDDGIPVIQSWQLFKTIKKAMQVLPDVAAQSLIIQSKNDKVIPKHSAEYIYQ
                     HLGSEHKHLIWLTKSDHAITVDYEKDEVTHHCINFFLKQ"
     gene            3087..3899
                     /locus_tag="PLJ75_08240"
     CDS             3087..3899
                     /locus_tag="PLJ75_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPD05240.1"
                     /translation="MSENNIILERKGSISIVTIDRPKRLHAFNDVMFDMLENVAARLG
                     ESLPRCIILTGSGDRSFSAGFDVQPDNPMVKRIVDAVSTKDIAPARETITRIRRAVDA
                     FVALPVPIIAALNGDAFGGGAELAIRCDLRVMARHAVICFSETKLGLMSDWGGGAALA
                     RLIGASRAADLLLTARKVLSDEALSMGLVNRVVDTGTALDEAIKIANLIAENGPHAVR
                     HVLALVRNSRNISYESSLEEEAERAIALIASGECFHGVSAFLEKRKPEFPDV"
     gene            4318..4878
                     /locus_tag="PLJ75_08245"
     CDS             4318..4878
                     /locus_tag="PLJ75_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD05241.1"
                     /translation="MELQQAIVERRSIRRFTDYYVTDEEIVQILEAARMAPSWANVQP
                     WEFIVVRDKSLIEKIVGTYSPTNPARKCSLASSALIVACAKTNVSGCKEGRDVTALAN
                     WYMFDLGMAVQNICLKAYELGLGTVVVGYFDHQQCNKLLQVPQDTTVVVTIPVGKPEV
                     SGKQGPPRKELMDCVYVNTYGNKFFK"
     gene            4889..5347
                     /locus_tag="PLJ75_08250"
     CDS             4889..5347
                     /locus_tag="PLJ75_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011306980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HPD05242.1"
                     /translation="MDIKDKWIYFAAPLFTAAERQFNETLALYIEQAGFAVYLPQKEC
                     AKISKSAEIYTQCLKGLDNAWCVVAVVDGCDTDSGTSFEIGYAYAKGIPVIGVRTDFR
                     QNADDGHLNLMLSQSIAMLCFVNSLEDASIDTVARCVKEKINALLIKVLQ"
     gene            5382..6005
                     /locus_tag="PLJ75_08255"
     CDS             5382..6005
                     /locus_tag="PLJ75_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05243.1"
                     /translation="MKRVLSCIAWFTGGILAALVVLLIYGLAITQNAIIQDTHKQYYH
                     ATRLVIYGDVVIGFLSDSSSQNKNLETSPHSFKVMLAGTIAQITFRPFESVYVPPLYL
                     GTYHINASGHDGILVLQVKDGLLYGAVRFPDWANGVWEPLKGVVIKNNTLYFTRSVTN
                     EKERLKTGAREYFTQYYVGTYKENGKKIVGFYKSRGAKYLFEAYRKK"
     gene            complement(6008..6958)
                     /locus_tag="PLJ75_08260"
     CDS             complement(6008..6958)
                     /locus_tag="PLJ75_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPD05244.1"
                     /translation="MNSLDTLLSQVTIFQRLSNEELSQLAPYFTRNNIGKDDVIFHEG
                     TYGNELFIVADGVIAAFIKLSDNSLREVARFSKGDFFGDMAIFENDVRSATCIAQTNC
                     TLLSLTADDFFAIMNNHPPIAIKIMYDMLTVISQRLLKTGSFLTEMIQWGEAARKRSI
                     IDEFTGVYNRSYFEDTIALLITQEKPFCLIMIDLDYFRKINEQFDHQVGDRAILAIVN
                     VLKKIFKESDAICRYGGDEFAIIVNGNNIDEAFNFAEQARVRVHHINCHELGMPNFTL
                     SISMGIAAFPQCGKDIIRTKADEALYMAKERGRNCVVIAS"
     gene            complement(6955..7881)
                     /locus_tag="PLJ75_08265"
     CDS             complement(6955..7881)
                     /locus_tag="PLJ75_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPD05245.1"
                     /translation="MDTKAIVKGVPLFSSLDDSEIQIIEKYCAHKELSRQEKLFSFDE
                     SFNLLCIVTSGSIGIYSNGAQNRLIARYIPNESFGELNLCGISLGKVEAIALDDAGVL
                     LFPATGTFVSFVTDHADIGAKIIYKLLSNISYRIRETNKLISQNYGWVSELRKAIYTD
                     RLTGLYNRQYCEELFRENKEYVIAIVKPDKFKMINDTCGHKAGDVTLVAIAQSLRRAL
                     PFATHIRYRGDEFMIVIAGDADMKKTAAAISEAIKAIDIHAITDGAINVITASIGISK
                     NTITQQAIENTYTIMWSAYNNGGNSIAMEGTK"
     gene            complement(8090..8329)
                     /locus_tag="PLJ75_08270"
     CDS             complement(8090..8329)
                     /locus_tag="PLJ75_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05246.1"
                     /translation="MAGKKASNTTTIKTTKRQSTEKKSKTNSIPIESNRIHEAIRIQA
                     FYNYLHRIMNNLPGDQLSDWLEAEKYIKKTINKQQ"
     gene            8732..9601
                     /locus_tag="PLJ75_08275"
     CDS             8732..9601
                     /locus_tag="PLJ75_08275"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPD05247.1"
                     /translation="MKKIAINLHPQDEKSLKLVAAFIKISHAKGFNVVLPDYALLHDD
                     FADYIANHEDYINADVAIAIGGDGTFIRTARMFAAGSVPILGVNRGRLGFLTEFLPQE
                     ALDFFDELASNTYTVSQRKMLKANIIRNQHQFAAIDFLNDAVITKGSFSRPIRIRLEL
                     NNEFVNCYAGDGLIIATATGSTAYSLSAGGPIVLPDDESVFVVNPVCPHTLAIRPMVV
                     PDSMVLSARVVTSIENLLLTIDGQEAIALQDTDVIHFSRSVHVIQVINHPSRRYFDVL
                     RQKLGWGANNEIE"
     gene            9605..11311
                     /gene="recN"
                     /locus_tag="PLJ75_08280"
     CDS             9605..11311
                     /gene="recN"
                     /locus_tag="PLJ75_08280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPD05248.1"
                     /translation="MLVELRIKNFILIDELSISFGKGLNILTGETGAGKSILIDALSG
                     VLGEKMSTDMIRSGYDRAVLEGVFDIGSLPQVKQLLELSGIDNDDDTLILRREIYANG
                     KGRCFANGVQIPLAKLKEIADYLVDIHGQNEHQNIVQVVKHRELLDNFGGLQSLVQQM
                     HDLHKTLTEIKEKIESFKIDEREKARRIDYLSHAIGEVEQANLQPNEEELLKNESSLL
                     SNAEKLFHELTTVQELMQDDRGVIQNLKKIEMSLGHMANYDPNLSPLLESVRDAYFSL
                     EDTHATLRDYARSIDFSPERINQVEERLALIASLKKKYGDTIEDVLAYAQNAKRELAT
                     ITSTEEALDKLQQEYDSTLTKAREVALLLSDKRLEAAKKLEGMVTSELADLGMQGTVF
                     RVSIKRELSPDGQIEANNKRYILYPHGLDRVEFLLSANEGEDLRQLRKAASGGEMSRI
                     MLALKKCILDADIVDSLVFDEVDAGIGGKTAEVVGKKLKTLAKQRQVLVITHLPQIAA
                     MSDNHYMVQKKKINARTTTLVEKLSDDKKVHEVARMLAGEVVTELSVKHAKELIKLAS
                     QQ"
     gene            11313..11900
                     /locus_tag="PLJ75_08285"
     CDS             11313..11900
                     /locus_tag="PLJ75_08285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HPD05249.1"
                     /translation="MKSDISINLSDFMTYLLGDIIQSTPAYQHIDMNRILVCSANNRN
                     SRGGAIFAKVVPLRFEDGVPLKKFKNHWYAFPKIMNGNHEILYLLYFYVPRYFDLPVY
                     EKLNVAFHELYHINPEFNGDVRRMGGYKFSHGYSKEHFNNHFKDEVDAYYNRVKNTSL
                     AIMLSMNFSDITKYFETVYARTMKVPKPQKIQEDN"
     gene            complement(11902..12177)
                     /locus_tag="PLJ75_08290"
     CDS             complement(11902..12177)
                     /locus_tag="PLJ75_08290"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPD05250.1"
                     /translation="MAKRLILHGVVQGVFCRYYCSETAKRLGLHGSASNLPDGTVEVL
                     LDTDDNTRLEAFIQALKTNPYDIRFYGSISTVDISDYHGTIRGDYRF"
     gene            complement(12479..14161)
                     /gene="nadB"
                     /locus_tag="PLJ75_08295"
     CDS             complement(12479..14161)
                     /gene="nadB"
                     /locus_tag="PLJ75_08295"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPD05251.1"
                     /translation="MAIEVYTSDFLIIGSGIAGLSFAIKASQLGTVHIVTKKKDFDSN
                     TNYAQGGIASVFDPHDSIELHIQDTLISGAGLCNEQAVRILAESGPKRVQELMDWGAH
                     FSTRANQNGSVTLDLGREGGHSHNRIVHAKDLTGQEVERALLDAIAKIKNITVFENHT
                     AVDLLTQHQLKLAGRHTNNSVSDITCYGAYILDNTSGVVHIFNAAYTFLATGGIGQIY
                     LHTTNPDIATGDGIAMAYRAGALIADMEFIQFHPTAFYQPGNKGRSFLISEAVRGEGG
                     ILVNSRGERFMDTVHPLKELAPRDIVARTIDMELKRLGDNCVFLDISFKDRDFLISRF
                     PTIYRYCMEAGIDMTKEPIPVVPAAHYLCGGIVSDLNGKTSIENCYVSGESACTGIHG
                     ANRLASNSLLEGIVFSHRAYCHIQTIFNSELKATIPEFPQWDKQGTFDLEEWILIQHN
                     VEDVKRIMWDYVGIMRSNLRLERAERRIALLEDEIQDYYKRSTVTSRLIELRNLVTTA
                     KLVVESALLRKESRGLHYNINYPRQDEKLKGSFVISCNQPPMFMKISDISFS"
     gene            complement(14169..>14870)
                     /locus_tag="PLJ75_08300"
     CDS             complement(14169..>14870)
                     /locus_tag="PLJ75_08300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD05252.1"
                     /translation="NNVTTAVHIVIDTTGIQWGLDFINRHTDKNIYIAMGIHPSSLAD
                     SEALHQLSVTIAHSKKTMPHKIFGIGETGLDYYRMRQPKSMQIESFHFQIQLAKEHNL
                     PVIIHSRDAMDDTYSIVKEYAPVHGIIHCFSGDHIDARKFIDLGFFISFAGNVTYKSA
                     TNLQQAASYIPMDTMLLETDAPFLTPVPFRGKPNHPGLVQYTYNYIALLRKISLSNCI
                     DNIAQNFSNVLKLNN"
BASE COUNT         4442 a         2793 c         3158 g         4477 t
ORIGIN      
        1 gctctactga aatacaggtt gtggtttcaa aatccattgt ttccctccag agtatatttt
       61 ttcaaagtgt agacctgagt atgatataat tgcaagcaat ttattttttt ataaattact
      121 atctatgagg ccattaaata tatattgtaa ctactcagct aaaatatttc acgtagaaaa
      181 aaattttgct attaatcgta gaggcatggt atagattatg tttcatgaaa tgagaagaag
      241 ctgaaaagat ataggtaccg agaaaacatg cagttggtga ttgtattctt caattagttg
      301 acaggatagg aagacttgaa agtcaactca ataagaacag tcataacagc agcaaaccgc
      361 catcaagtga tggattgaag aaaaaggcaa ggaagtggag tcaaaggaag cgaacgggga
      421 aaaaaccagc gggcagtctg aggcatctga tgcgataggc atagtaccat cttaccaaca
      481 ttgaaaggag catctgcact ccgcaaaagt attggagtta taatgttgct attccatttg
      541 gtaacaatct ttcggaaaga gatatccgta tggttaaagt acaacagaaa atttcaggac
      601 tattcagaac gtttagtaat gctgaacaat tttgtattat ttcgaagtta catcccgaca
      661 gtaaaaaaag cagtgtttta gtgttattga tgcactcgaa cgcatactta atggtaagca
      721 ggtttatcgt gagtttgttt cttaatagct gagtagttaa aattaatgtt tatggatgct
      781 ccattgtaat gaataaaaaa ttgctttaaa gaaaactcta tggttatata tagttccagt
      841 tacatgcttt tttataaatg ataataacta tcacaatagc attaaggctt ttgtatgata
      901 ccgtgtttct gcttgtataa cattttcaag gcacgttgta accggactaa tttcgggctt
      961 ttctttttct gatgcttcct tcaatgccat tgcaaaggca gtccatgctg cagcagtttg
     1021 ttgcatatag taaggtaatt gcaatgattc tatgtctggc aaataagcgg tagcttcttt
     1081 aagaaaatcg gcatatatca gtcggaaacc tccgccacct gttccacgcc tttcaatgac
     1141 ctggtatgca aagcgaaatg cccacttcca gtcttcaaat tgagtccata gttcaagctc
     1201 gttataaaag gttgtaagcc cctgaatacc ctgtgtggat attgatgtat caagtataac
     1261 acggctattg tagataattg cattacggat agtgtcttgc atatttactg gagctttcat
     1321 catggcaggt gctatgcaat tgccatggat tttaaaaaaa ttgtcttcgc agaaacgtgc
     1381 ctgtgcaagg tcgctatatg caacttcaat taatgcaggg cgttcagtat cggtgatgta
     1441 aaacaccttt ttatcatcat catagcccca gacagtaatg acatgcccgg ggaaatgagt
     1501 ggatgactta tagtaaggaa gatagtataa atcggtttgt actattgcag gtaaaccagc
     1561 gtcaagtttt ttaataagtt cacgatgact ttgctgttgt gtttcatatg tttcccatgt
     1621 aacaggcatg ccaatatgca aaaaaaaccg ttcttcaata tctgctgaac gtacatgaat
     1681 catgcgcggg ggattattgc tttttatata ccatattccc aaacctgcac caatgccaaa
     1741 acagaaagcc tcactccacc ccaatccatg aaatgcagca aggtttcgta atcctgtact
     1801 ggcacagtgt ctgccgggtg tatggagatt tttaagtaat acgcgcataa ataattcagt
     1861 cattatagtt tgtcatggtt cttgtaccta tattattctg tttaaagaat aatgtcaact
     1921 gtaatatagc aggtaaaagc tatagttgac ataacaccat gcatggtgtt taattattgt
     1981 gtttaatatg aagaataaaa taaataggag agatagttat ttgattaatt attttcttta
     2041 tattaatgtt ttttatagtt tgtgaaaagg aggagttatt atgcaatcaa aaaaaatggt
     2101 aattacagtt cctgaaaaag ccagagcata ttttaaaaaa ggatcagata atctgggtat
     2161 tttgctcctt cacggattta ccggtacccc tgctgagatg cgatacatcg cagagaagct
     2221 tcacaatgca ggtgtaacag tctatgtacc acggtatcct ggtcatggca ccagtttggt
     2281 tgagatggca aataccagcg ttcaccattg gtttacagca gcacgtgaag cattgtttga
     2341 attaaagtca cattgtcggg aggtctacgt cgcagggctt tctatgggtg gtatttttgc
     2401 aattttgctg gcaaaagaat ttgatattaa aaaaattgct cttatgtcgg taccctgcac
     2461 acttaaagaa aaaactattt acataacgcc attggttgga cgttttaaaa agattatatg
     2521 gattcccaac aaaactaaag gtgtgtgcga tgaacgggca cgcaaagaac atatctgtta
     2581 tgatgatggt atcccggtca tccaatcgtg gcagcttttt aaaaccataa aaaaagctat
     2641 gcaggtactg cctgatgttg ctgcacaatc gctcattata caatcaaaaa atgataaagt
     2701 tatccccaaa cattcggctg agtatatcta tcaacatctg gggtcagagc acaaacatct
     2761 gatatggctt acaaagagtg atcatgcaat aactgttgat tatgaaaagg atgaagttac
     2821 acatcactgc atcaattttt ttttaaagca gtgacctgtt catgtgattt ctttgtgtac
     2881 atcaatgcgc aggctttggg ttatattttt ttatgagcat gaataatctt tttgacaaat
     2941 atgggagata gttattcatt aacaagcaac atctgttaac cctgacaccg gtaagctcgt
     3001 gagtaaaggg taacctctgt attcgccagg agagctgttt cgtaatgatg cgcaagtgtc
     3061 aggtccttat gtgtgaggag ggtacaatgt cagagaacaa tattatcctt gaacgaaagg
     3121 gttcaatttc aatagtaacg atcgacaggc caaagcgcct ccatgctttt aacgatgtga
     3181 tgttcgacat gcttgagaac gttgccgcca ggcttggcga atcattgccg cgatgcatca
     3241 tactaacggg aagcggagac cgctctttca gcgcaggatt tgacgtacaa cctgacaatc
     3301 ccatggtgaa aagaattgtg gatgcggtta gcacaaagga catagcgccc gctcgggaaa
     3361 caataacccg tataagaagg gcagtcgatg cgtttgttgc cctgccggtt cccattatcg
     3421 cggcattgaa cggcgatgct ttcggaggag gggcagagct tgccatcagg tgcgatctga
     3481 gggtaatggc gcggcacgcc gttatctgtt tttcggaaac aaagcttggc ctcatgtccg
     3541 attggggagg aggtgcagcc ctggcaaggc ttattggtgc ttcaagagcg gcagatttgc
     3601 ttctgactgc acggaaggtg ctatccgatg aagcactttc aatgggattg gttaaccgtg
     3661 tcgtcgatac cggtactgcg cttgatgagg ctatcaaaat cgccaatctt atcgccgaga
     3721 atggccccca tgcggtacgt catgtgctgg cgcttgtccg aaacagcaga aatatctcat
     3781 acgaaagctc tctggaagag gaagccgaac gggcaattgc tctcattgcc tctggtgaat
     3841 gcttccatgg tgtctcagct ttcctggaaa agcggaagcc ggaatttccc gatgtgtgat
     3901 gagatgaaat agggacaata ataaataata gaagtgtaat aattctttgt atcaaatcac
     3961 attcaaaaac ttctctggta gtatctttaa aggagtgaga agcgtgatgc tcatgatgat
     4021 gaaaagcatg ctgtttttca gttcttcaat gtgtatgcat gatacgccga taaagcaagc
     4081 gttctgtgtt tatacatctg atatattgat attgtgcact ctatagccgg tcgttttttg
     4141 acatttcttt ctgtatttcc tatggttgca ttgtgcatgt tacctatccc acaaatgttc
     4201 ttgtgtgatt gtgcacaaat acattataat attcattttt gctttacata ataatgccta
     4261 ttttatacta tcaacattga tgcattttta tattgtatgc cacatgagga ggtagctatg
     4321 gaattacagc aagctattgt tgaacgcagg agcatcaggc gttttactga ttattatgtt
     4381 actgatgaag aaattgtgca gatactggaa gctgcacgca tggcaccatc gtgggctaat
     4441 gtacagccat gggaatttat tgttgtacgt gataaatcat taattgaaaa gattgttgga
     4501 acatattcgc caaccaatcc tgcacggaaa tgttcattgg cgtcttcagc gctcattgtg
     4561 gcatgtgcta aaacaaatgt ttcgggttgt aaagaaggca gggatgttac tgctctggca
     4621 aactggtata tgtttgattt aggtatggcg gttcaaaata tctgcttaaa agcttatgaa
     4681 ctgggattgg gcacggtagt ggtgggttat ttcgatcatc aacaatgtaa caagctatta
     4741 caggtgccac aggataccac cgttgttgta acgatacctg ttgggaaacc agaggtaagt
     4801 ggtaagcagg gacctcctcg aaaagagttg atggattgtg tttatgtgaa tacctatgga
     4861 aataaatttt ttaaataagt aaaacgatat ggatataaaa gacaaatgga tctattttgc
     4921 tgcaccactt tttacagcgg ccgagaggca gtttaatgaa acactggcat tgtacattga
     4981 gcaggctggt tttgcggtgt accttcccca aaaagaatgt gccaaaatca gcaaatctgc
     5041 ggagatatat acgcagtgct taaaaggact tgataatgca tggtgtgttg ttgcagtagt
     5101 ggatgggtgt gatacggatt ccggtacaag ctttgaaata gggtatgcct atgcaaaggg
     5161 gatacctgtt attggcgtca gaactgattt cagacaaaac gctgatgacg ggcaccttaa
     5221 tctgatgctg tcgcaaagca ttgctatgtt gtgttttgtc aacagccttg aggatgcatc
     5281 aattgatact gttgcacgat gtgttaaaga aaagattaat gctctgctca tcaaggtgtt
     5341 acaataaaat taaaataaac atggaataag ggtggtttac catgaaaaga gtactatcat
     5401 gtattgcatg gtttacgggg ggaatacttg ctgcacttgt tgtactgctt atctatggat
     5461 tggcgattac tcagaatgct atcatacagg acactcacaa acaatactat catgccacaa
     5521 ggcttgtcat atatggcgat gttgttattg gatttttaag cgatagttcc tcgcaaaata
     5581 aaaatttaga aacatcaccc cattcattca aggtgatgct tgccggaact atcgcccaga
     5641 ttacgttccg tccttttgaa agcgtttatg ttcctccact ctatttaggt acataccata
     5701 tcaacgcttc ggggcacgat gggatattgg tgttgcaggt taaggatgga ttactctatg
     5761 gggcggttcg cttccctgat tgggcaaatg gtgtatggga gccattaaag ggtgttgtaa
     5821 taaaaaacaa tacactctat tttacacgtt cagtgacaaa tgaaaaagaa aggttaaaaa
     5881 ccggcgcacg agaatatttc acacaatact atgttggtac ctataaagaa aatggtaaaa
     5941 agatagtggg cttttataaa tcacgtggtg caaaatatct ttttgaggct taccggaaaa
     6001 agtaatatta cgaagcaata accacacagt ttctgccacg ctcttttgcc atgtagagcg
     6061 cttcatctgc ttttgtacgt atgatgtctt tgccacattg cgggaatgct gcaatcccca
     6121 tgctgatgga aagcgtaaag ttgggcattc ccaattcatg gcaatttata tgatgaacac
     6181 gtactctggc ttgttcagca aaattgaatg cttcatcaat attgttgcca ttgacaatga
     6241 tagcaaattc atcgccaccg tagcggcata ttgcatcgga ttctttaaat atttttttaa
     6301 gcacattgac tatagcaagt attgcgcggt cgccaacttg atgatcaaac tgttcattga
     6361 tctttcggaa ataatcaagg tcaatcataa tgaggcagaa tggtttttcc tgtgttatca
     6421 aaagtgcaat agtatcttca aaataactgc ggttgtaaac accggtaaac tcatcaataa
     6481 ttgaacgctt acgtgctgcc tcgccccatt gaatcatttc ggttaaaaac gatccagtct
     6541 ttaaaaggcg ctgtgagata accgtgagca tatcgtacat gatcttaata gcaatgggtg
     6601 gatgattgtt cattatggca aagaagtcat ctgcggtaag cgaaagcagg gtgcagttgg
     6661 tttgtgcaat acaggttgct gaacgtacat cgttttcaaa gattgccatg tcaccaaaaa
     6721 aatctccttt actgaaacgg gcaacctcac gcagagaatt atcggacagc tttataaatg
     6781 cagcaatgac accatcagct acaatgaaaa gctcattgcc atacgtccct tcatgaaaga
     6841 tgacatcgtc cttaccaata ttatttctgg taaaataggg tgccagttga gataactctt
     6901 cattgctgag cctttgaaaa atagttacct gtgacagtaa agtatccagg ctgttcactt
     6961 tgtaccttcc attgctattg aattgccacc attgttatat gctgaccaca ttattgtata
     7021 cgtattttca atagcctgtt gagtaatggt atttttactt attccaatac ttgccgtaat
     7081 gacattgatg gctccatccg ttattgcatg tatatcgatg gctttgatgg cttctgaaat
     7141 tgctgcagca gtttttttca tgtcagcatc acctgctatc acaatcataa actcatcacc
     7201 cctgtagcgt atgtgtgtgg caaacggtag agctctgcgc agggattgtg ctattgccac
     7261 aagcgtgaca tcacctgctt tgtgtccgca ggtgtcattt atcatcttaa atttatcagg
     7321 ctttacgatg gcaatgacat attctttatt ttccctgaat agctcttcgc agtactgtcg
     7381 gttatacagg ccggtaagcc tgtcggtgta gatagccttt cgcaattcac ttacccagcc
     7441 gtaattttgt gaaataagtt tattggtttc gcgtatcctg tatgatatgt tcgatagtaa
     7501 cttataaatg atctttgcac caatatcagc atgatcggtt acaaatgata caaaggtgcc
     7561 agttgccgga aacagaagca caccggcatc atcaagggca atggcttcaa ccttccccag
     7621 cgatatgccg catagattaa gttcgccgaa actttcattg ggtatatacc gggcaattaa
     7681 tctgttttgt gctccgtttg aataaatgcc tatgcttcct gaagtgacta tgcacaacag
     7741 gttgaatgat tcatcaaatg aaaaaagttt ttcctgcctt gataattctt tatgtgcaca
     7801 atatttttca ataatctgga tttcgctgtc gtcaagcgat gaaaatagcg gcacaccttt
     7861 tactattgct tttgtatcca ttataccttc tgttgacttc ttctatactg tagtgaacac
     7921 tttttcatca tgtacctttg aatgcaataa aaaaataatc agtaactatc gccagaagca
     7981 tataaaaata aaattgctcc cttgcaacgt tgcatttttt aaaaggcatg tactgggtat
     8041 agagtattat ttattttcag tgcagatgca aggtcaaaaa aagatgcatc tattgctgtt
     8101 tattgatggt cttttttata tatttttcag cttccaacca atccgatagc tgatcacccg
     8161 gtaaattatt catgatacga tgtaaatagt tatagaatgc ctgtattcgt atggcttcat
     8221 gaatacggtt actttcaatc ggaattgaat tggtttttga ttttttttca gtgctttgac
     8281 gctttgtggt tttaatggta gttgtattgc ttgctttttt cccggccata aggatacctc
     8341 cttcccaaat tgtgtattga atagtttata ctaagggaat aaatagtata aaaaaataat
     8401 attgtcaatc aattttatag aaaataaaca aaactccatt gccataattt cattgacata
     8461 atctggttaa ccggtgtagt aataactcat cataagtaaa tgaacggaca gcttttgata
     8521 acgattgtgt cggcatccat atatgatgaa aatagctgga ggcagtcatt ctacatcatg
     8581 gttcagaata taacgttgta tatcagaatt ccggaacaga tccggaatga cagaggtggc
     8641 ataatgctat tttctatttt caaagcagtt aactcaatag taagtatcat atttttactc
     8701 ttataaagct aatagtggta aaaggaaaac aatgaaaaaa atagctataa atttacaccc
     8761 ccaggatgag aaatcactga agcttgttgc agcattcatt aaaatttcac atgcaaaagg
     8821 cttcaatgtt gttttacctg attatgcgtt gttgcatgat gattttgctg actacattgc
     8881 aaaccatgaa gattatatca acgctgatgt agcaatagcc attggtggcg atggtacctt
     8941 tatacggacg gctcgcatgt ttgctgctgg aagcgttcct attctgggcg tcaatagagg
     9001 acgattggga tttttaaccg aatttcttcc tcaggaagca ttggatttct ttgatgaatt
     9061 agcgagcaat acctatactg tttcgcaaag aaaaatgctg aaggctaata taatccgcaa
     9121 tcagcatcag tttgccgcaa tagatttttt aaatgatgcg gttatcacaa aaggttcctt
     9181 ttcacgacca atcaggatac ggctggagct aaacaatgag tttgttaact gctatgccgg
     9241 tgatggtctt atcatagcta ctgcaacggg ttctacggca tattcgcttt cggctggcgg
     9301 gccgatagtt cttcctgatg atgaatccgt atttgttgtc aatcccgtat gcccgcatac
     9361 ccttgccata cgtcccatgg ttgttcctga ttcaatggta ctatctgcca gggtggttac
     9421 cagcattgaa aatttattat tgacaataga tggtcaggaa gctattgctt tgcaggatac
     9481 cgatgtgatt catttttcac ggtcggtaca tgtgatacag gtgatcaacc acccatcaag
     9541 gcgttatttt gatgtattgc gtcaaaaatt aggatggggt gctaataatg aaatagagtg
     9601 aactatgctg gtagaattac gaattaaaaa ttttattttg atcgatgagc tatcaatcag
     9661 ctttgggaag ggattaaaca tcttaaccgg cgaaacgggt gcgggtaaat ccatccttat
     9721 tgatgcgctc tcaggagtgc tgggcgaaaa gatgtcaacc gatatgatac gctcaggata
     9781 tgacagggca gtgctggagg gcgtttttga cataggttca ctgccgcagg taaaacaatt
     9841 gctggaacta tcgggtatag ataatgacga tgatacattg attttgcgcc gtgagatata
     9901 tgccaatggc aagggaagat gttttgccaa tggtgtgcag attccgcttg caaaattaaa
     9961 agagatagct gactatttag tagatattca tggccagaac gagcatcaga atatcgttca
    10021 ggtggtaaaa cacagggagc ttctggataa ttttggaggg ttgcagtcac tggtgcagca
    10081 gatgcatgat ttgcacaaaa cattaactga aatcaaagaa aaaattgaat catttaagat
    10141 agatgaacgc gaaaaggcac gccgcattga ttatctttca catgctatag gcgaggttga
    10201 acaggcaaat ctacagccaa acgaagagga actgctcaaa aatgagtcaa gccttttgag
    10261 caatgcggag aagctttttc atgaacttac tactgtgcag gaattgatgc aggatgaccg
    10321 gggggttata caaaatttga agaagattga aatgagtttg ggccatatgg caaattatga
    10381 tcctaatttg tcaccgctgc ttgaatcggt gcgcgatgcc tatttttcac ttgaggatac
    10441 tcatgctaca ttgcgtgatt atgcccgttc tatcgatttt tcacctgaac gcatcaacca
    10501 ggttgaagaa cgtcttgcgc tcattgcaag cttaaagaaa aaatacggcg ataccataga
    10561 ggatgtactg gcatacgcac aaaatgcaaa gagggaactt gcaacaataa catccaccga
    10621 ggaagcgctt gataaattgc agcaggagta tgatagcaca ttaacaaagg cacgcgaggt
    10681 agcgctgttg ctttcggata agcgccttga agctgctaaa aaattagaag ggatggtaac
    10741 atctgagctg gctgatctgg gaatgcaggg cactgttttc agggtttcca ttaagcgcga
    10801 gttaagtcct gatggacaga tcgaagcaaa caataaacgg tatattttat atccgcatgg
    10861 tcttgacagg gtagagtttt tgctgtcggc aaacgaaggc gaggatttgc ggcaactgag
    10921 gaaagcagcc agtggtggtg agatgtcgcg catcatgctg gcattgaaaa aatgtattct
    10981 tgatgctgat attgttgact cgctggtgtt tgatgaggtt gatgcaggca ttggaggcaa
    11041 aacagcggag gttgttggga aaaagttgaa aacattagcc aaacagcggc aggtgcttgt
    11101 tataacgcat cttcctcaga tagcagcaat gtctgataat cactatatgg ttcagaagaa
    11161 gaagatcaat gcacgcacaa caacactggt ggaaaagctt tcggacgata aaaaagttca
    11221 tgaggttgcc cgcatgttag ctggtgaggt ggttacggaa ctatcggtta aacatgcaaa
    11281 agagttaatt aaactggcat cacagcagta atatgaaaag tgatatttct atcaaccttt
    11341 ctgattttat gacatacctg ctgggtgata ttatacaatc aacgcccgca taccagcata
    11401 ttgacatgaa tcgtatcctt gtttgcagcg ccaacaatag aaacagcagg ggcggtgcaa
    11461 tatttgccaa ggttgtaccc ctgcgatttg aagatggtgt gccgttaaaa aaatttaaga
    11521 atcactggta cgctttccct aagattatga atggtaacca tgaaattttg tatttacttt
    11581 atttttacgt cccgcgctat tttgatctgc ctgtgtatga aaagctcaac gttgcttttc
    11641 atgaactata tcatatcaat cctgaattca atggcgatgt gcgtcgtatg ggtggatata
    11701 aattttcaca tggatattca aaggaacatt tcaacaacca ttttaaagat gaggtagatg
    11761 catattacaa cagggtaaaa aacacctcgc ttgctattat gttaagtatg aattttagcg
    11821 atattacaaa gtactttgaa acggtgtatg cgcgtaccat gaaagtgccc aaaccgcaaa
    11881 aaatacagga agacaattaa gctaaaagcg atagtcaccg cgaatggttc catgatagtc
    11941 ggatatatcc accgtgctga ttgatccata aaaacggatg tcgtaaggat ttgttttcag
    12001 tgcctgtata aatgcttcaa gccttgtgtt atcatcagta tccagtagaa cctcaacggt
    12061 cccatcaggt agattgctgg cacttccatg gagccccaaa cgctttgctg tttcgctgca
    12121 gtaataccga cagaatactc cctgaacaac accgtgaagt attaaccgct ttgccatagt
    12181 accattctcc tgaaatagaa ttatgagcca ttgttatatt aatagcacga tgcactctct
    12241 gtcattctga aaggtgtaac cgtgaataat ctcttctttt aacaacgagc ttctttattt
    12301 cgacttcggc tcagcacagg aaatctgtat gattgagaaa cttcggtagc acctcataac
    12361 aaaatttcaa agtcctgcta tagtctattt tgtcacagtg tcaatgataa aataatgggc
    12421 atagtactat tacagtaact atcgccataa aaacattgca gtaaaagcaa taagcacatc
    12481 acgaaaatga aatatcagaa attttcataa acattggggg ttgattacac gatataacga
    12541 agcttccctt taatttttca tcctgtcggg gatagttgat gttgtaatgt aaaccacggc
    12601 tttctttgcg caacaatgcg ctttccacaa caagctttgc tgttgtaacc agattgcgaa
    12661 gttctattaa ccgtgaggtt acggtgcttc gtttgtaata gtcctgaatc tcatcttcta
    12721 acaaagcaat acgccgctca gcacgctcca atctaagatt tgaacgcatt atacctacat
    12781 aatcccacat aatccgctta acatcctcga cattatgttg aattaatatc cactcttcca
    12841 gatcaaatgt cccttgtttg tcccactgag gaaattctgg tatggttgct ttaagctcag
    12901 aattaaaaat tgtttgtata tgacaatacg cgcgatgcga aaaaactatc ccctctaaca
    12961 gcgaattact ggcaagcctg ttagccccgt gaataccggt acaggccgat tcgcctgaaa
    13021 cataacagtt ttcaatagat gtcttcccgt ttaaatccga aacaatacca ccacacaggt
    13081 agtgagcagc cggaactacg ggtataggtt ctttcgtcat atcgatgcca gcttccatgc
    13141 aatagcggta gattgttgga aatcgtgaaa taagaaaatc tctatcctta aatgagatat
    13201 caaggaatac acaattatcg ccaagccgtt ttaattccat gtcaatggtt cttgccacta
    13261 tatcgcgtgg cgccagttct ttcagcgggt gcaccgtatc catgaaacgt tcaccacggc
    13321 tgttcacaag gatgccaccc tcaccgcgaa cagcttcgct gattaaaaat gaacgtcctt
    13381 tgttaccggg ttgataaaaa gctgtgggat gaaattgaat aaactccata tcggcaatta
    13441 aagcccctgc tcggtatgcc attgctatgc cgtcaccagt tgctatgtca ggatttgttg
    13501 tgtgaaggta aatctgacca atcccaccag tagcaaggaa tgtatacgcc gcattaaaaa
    13561 tatgaacgac gccgctggta ttatcaagga tatacgcacc atagcatgtt atatcactaa
    13621 cactattatt agtatgcctg cctgctaatt tcaattgatg ttgtgtcagc aaatcaacag
    13681 cggtatgatt ttcaaatacg gttatgttct ttatctttgc aattgcatct aaaagagcgc
    13741 gctccacttc ctgtccggtg agatcctttg cgtggactat cctgttatgt gaatgtccac
    13801 cctcccttcc taaatcaagt gttacactac cattttgatt tgctcgtgtg ctgaaatgtg
    13861 cgccccaatc cataagctct tgaacccgtt ttggtccact ttcggctaat atacgcaccg
    13921 cttgttcatt gcataaaccg gcacccgaaa ttaatgtatc ctgtatatgt aattcaattg
    13981 agtcgtgagg atcaaatacc gatgcaatgc cgccctgtgc atagttggtg ttggagtcaa
    14041 aatccttttt tttggtaaca atatgtactg ttcccaactg tgatgcctta atcgcaaagg
    14101 ataaaccagc aatgccactt ccgatgataa gaaaatctga tgtataaact tcaattgcca
    14161 tgatactatc aattattaag ctttaacaca tttgaaaagt tctgagcgat attgtcaata
    14221 caatttgata aagaaatttt tcgtaataag gcaatatagt tataggtata ctgtacaagc
    14281 ccgggatgat tgggttttcc tctgaaggga acaggtgtta aaaagggcgc atcagtttca
    14341 agaagcatcg tatccatagg gatatatgaa gcagcctgtt gcagattggt ggcactttta
    14401 taggtgacat tgccagcaaa tgatataaaa aatccaaggt caataaattt ccttgcgtca
    14461 atatgatcgc ccgaaaaaca atgaataatt ccgtgcactg gtgcatactc ttttactatt
    14521 gagtatgtat catccatggc atcacgcgaa tgaataataa ccgggaggtt atgctccttt
    14581 gcaagctgta tctgaaagtg gaatgactca atttgcattg atttaggctg gcgcattcta
    14641 taataatcta aacctgtttc accaatgcca aaaattttat gcggcatggt ttttttacta
    14701 tgggcgatag ttactgataa ttgatgcaat gcttcagaat cagctaacga tgatggatgt
    14761 attcccattg caatgtagat atttttatcg gtatgcctgt taataaaatc cagtccccac
    14821 tgtatccctg ttgtgtctat gacaatatgg acagcagttg taacattatt
//
LOCUS       DAOOJH010000057        14792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3894_length_14792_cov_9.040035, whole genome shotgun sequence.
ACCESSION   DAOOJH010000057 DAOOJH010000000
VERSION     DAOOJH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14792
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_14792_cov_9.040035"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1348
                     /locus_tag="PLJ75_08305"
     CDS             <1..>1348
                     /locus_tag="PLJ75_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD05253.1"
                     /translation="DTTFYVLDEHNLIVNGSYSYHPPLKTIVFTPAGGFAWNGVYTAV
                     VTTGIYNALGLHMKQGYQWSFRIAPFYDTQPPAIDTNSVHPAGDNVSCHCIISFQIID
                     NGHIDIGSINGTNIVVSYKNGSNTLEVPCTYQYHASVKEIRCIPQSPLLEGKLYTVQV
                     HEGLQDESGNALANPCQWSFTTEVIPPQVLSVTPYDGASNVSVGEHIIIQFSEPVQQS
                     TLSQGITVSDSYGNDVDGTLEYDPNTYVATFIPHHLEYVTQYTVSAGEAINDISGIPL
                     QPVYSSTFTTMQEDISPEIIAITPDSPVNTDSTFQILFSESMDEASLVEHIGVRDSYG
                     NEIPVHINYDDAIYLCTVTPDRLIGFYNYTVYITTGVTDIHGNPLIHNYEKLYTTNPA
                     PVNTVVMLYAPVDYEGGDYIDGDIDELIDAQVDHSLVRIVALVDYPYDGNTVIVES"
     assembly_gap    1349..1448
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1449..2370
                     /locus_tag="PLJ75_08310"
     CDS             <1449..2370
                     /locus_tag="PLJ75_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05254.1"
                     /translation="ARFDSGFYYPIPWYVDWIDFLNKANAGGLLIGNFNRMVCKTVMA
                     SGHQGYGIGIMFPTKTPEDWYNNINPYGIDFIGDYGWDEFLQGKHFGLYADSNEPLND
                     DGFSAMTISSTDVTRQAIYYNYIHDPDDTDIYTVTVNQHAGAGNAVTSSDEIPINNEK
                     IKWQTVSCSYDGDVIAATAQNRYIDPLQDAGTPWLQRTGTVAQNWSRTQTSALESAVA
                     EVVDGTLTIAVTGIPENCELAMIIKDHNGTIIREAIRSGLGGTLRCDLRIYTGVYVQY
                     RVYLVPVNIMGWSMNTHEHYTIQLSYQKNE"
     gene            2387..3007
                     /locus_tag="PLJ75_08315"
     CDS             2387..3007
                     /locus_tag="PLJ75_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05255.1"
                     /translation="MENKNKRKILINKYYQLKTSFGVAGLFALSMGILFIISSSMLVW
                     NNISIAKAMTHQMDLQKVQEDILQSIEMLAKYGNKKNLIFEAYKAIGDLKRNTDALKE
                     SNELLRRSIAVNQYLMWVNGILGVALIIAVFVIVLRRTHRVAGPMFLMKRYMNDIIQG
                     RVPEVRALRKDDEFKDVFETFVSMIETLHLNRLKRFQKSKTKSSKG"
     gene            complement(3004..4062)
                     /locus_tag="PLJ75_08320"
     CDS             complement(3004..4062)
                     /locus_tag="PLJ75_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05256.1"
                     /translation="MYKPRYTFIVLLLFCMLLPYKAYASYYPFILQSKPPVCNHCIQP
                     SETVTVLHHNLIQQLLSSGVFISDSSSYVIPAGAINIPPAGELIRTLTVEITEQQSIL
                     YKELTPYLKEKIEQTSYSITVTVSSGTDPQEYVIQKENLTSQQLEQLYVTIAHDIIDF
                     YSSRRIPVLYQAAFAITLTAITLSPVYIDAFKELKDYAHSGSGFQASTCIENTKLPSL
                     VVIPALTWFMLHDPVVTVNYWHSISFMMQAGYRIKLPLSLSCTPFIGAGYTMHIISGD
                     THHYYPPYSYSQSIYYNPQIAAGIILALMLDPQLSLSSSAAYTTYFESSSHSSYFMYT
                     LGFTFHCNWQLYQTMVYH"
     gene            4217..>4911
                     /locus_tag="PLJ75_08325"
     CDS             4217..>4911
                     /locus_tag="PLJ75_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05257.1"
                     /translation="MFWFYGKNVHAIQLLMYSRIRSAGVRVATVLMLVVLLHCYGCTD
                     VNYPTDVLDKSPYDMSGGSFVIDGDAPFAYSQQVMLSSHVDNAYWMQFSNDAIRWVDW
                     VAFDNHSMWLLPLQYGETTVYAHYKNIRGDIVAYQDSIFFVERLVNNTSIQLGGDVAV
                     SNDGAIVAAGTLYGRANAVYVFHKQSIDWQGTKIKPDDIVDGDRFGYCVALSGDGRWL
                     FAGAPGKNAVYVY"
     assembly_gap    4912..5011
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5012..5523
                     /locus_tag="PLJ75_08330"
     CDS             <5012..5523
                     /locus_tag="PLJ75_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05258.1"
                     /translation="GDILPGDGQHYAFFGQSVAVTDDACWCVIGSPGYDILPNDDKGC
                     FYIFKRNADNTFLLLTQIKNTGGSRGDALGGAVAMSRDGRTIVASMHLYDTGLNAVDR
                     GLVQVYSFDGSGVEHSDTYSPDDRDTTCYFGNAIAASNNGVIVIGAPYAYRDTAGDNG
                     GVVYIRRWL"
     gene            5567..6238
                     /locus_tag="PLJ75_08335"
     CDS             5567..6238
                     /locus_tag="PLJ75_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05259.1"
                     /translation="MRRLVMMLLIMGMAGSVSAQELQQKDEMKKQAPLVKTFFAMAGT
                     MYGFAYPAGTIDAINGHVEREKDTYFGGNIGIGYQLSPLFLIHGGFTLWDAEVSVKRN
                     LLGQKLETRYCLDTCALHAGIMMVYKWFYADCMLFTELPLEDDTEKVYLSGEYMQTNR
                     LKSRYEYGGFLGLGVRIPVMEQFFVLAGIYGGGGFVPSLESSRGDELYLINATIRISV
                     MVYVL"
     gene            6304..7728
                     /locus_tag="PLJ75_08340"
     CDS             6304..7728
                     /locus_tag="PLJ75_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05260.1"
                     /translation="MKVYKGILLILLWSVYTGCSTADTESLSIEGQVMLCGEGMEGVC
                     VALQHPQYGSVETYTDDLGMYHFKEAWAGDYSVTPFVDGCEIVPVKREVSVFDNTVVN
                     DFIIRPLWERTYGSDYQDVVNAMTNTRNCGYVYAGSTDLHGSHEAFAIRLNAMGTMMW
                     YNTYGRDVYHSANAVLALDDGGCMIAGSKGSSQTNVDIWVAKIDGNGALQWQRTYGGN
                     QYDEARSIVNVNDGYCIAGCTESFGNGKEDVYVIKINDDGTVIDSTYFGGVNDDRALK
                     IIKCKNDSGFLIAGITEYENQLVADSWVVKLNPDLELSPNGWTKEYAIALYDCATDIL
                     ELDDGSIIVCGWGWDVENASRIWVSKLRGDGSVEEWTKEYYIGASCAVNSMIQRADGS
                     IVLTGYTMTALNDEKDILLLALDNEGNVLWTQVYGSGNEINDEGKAMVLAGDGGISVA
                     CDMKVSGVAGDVHCLKVNDKGLLR"
     gene            7810..8337
                     /locus_tag="PLJ75_08345"
     CDS             7810..8337
                     /locus_tag="PLJ75_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05261.1"
                     /translation="MKKQDYNNSTIYFVTVTTKGRVCLFGTIDEGQMHLNEMGIIVNN
                     CWLDIPENFPDAVMDVFVVMPNHVHGIIMVNDSQKKTKGAKKSIHKPEKRLLEAIIGQ
                     FSYKSTIEILTMSRREKADEWRWSIWDDNYYTRIIESEHYLNILRWYIIQNPYRWDVD
                     RENPYRNIQKRRDMP"
     gene            8334..>9657
                     /locus_tag="PLJ75_08350"
     CDS             8334..>9657
                     /locus_tag="PLJ75_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05262.1"
                     /translation="MKKACFLLLLCTILLLSCSGSKHKNEAIINGIDIPVRIYPAGVT
                     VAGEDGREQPSGDSILAYDTSITSTWHVLEKSVITFQCNHTYIDTIQLYDAGGVTLKL
                     YGDSADGRLLATIDDTTVRNDGWITVSIGREVDALVLVVEPAGSDAHIAHIELWGSAD
                     SVAGILLHYRNVPALDETSVDETVADIYPTLLKAGENSYEATVNITNPAMYRAVYVVY
                     TGRGIYRPVSVMMKVNECDWLGGLTIPESAGQETIQRQQSCEINPLWLTSGSNTLHFK
                     GVTNEVSLQEVTVVGIKDTGYNEPRQGTEVLTDGNGMTGVPATGGAIVTYNRLMRMYG
                     VLVYAGDNAGSIQVVCKGGAGISEGGMCTATQAGWYYIPLNKPVTGNEIAVTLANTAC
                     VIGEVAACASAAEGAHPAIVVSYPRNGEYFGRTAYIQGFITPYATDDGV"
     assembly_gap    9658..9757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9758..>14792
                     /locus_tag="PLJ75_08355"
     CDS             <9758..>14792
                     /locus_tag="PLJ75_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HPD05263.1"
                     /translation="TGLTVTVSPDSAKSIYYQGVQIEIPEKAVDEDVTITVVPLDEQQ
                     IQNLTPGMHNVTAPAAGYRFLFNGKAHGSFKKPVTISIPYDKHKLLSGSGEHEIRMFY
                     YDDGMGVWKQMEASRVVTAKSADYILYSRNITSTQAGGYVVVESTHFTDFINATLTVP
                     EHPEALQFNPNSIKDIKAGEPLAGMQLIQPPQSSNTGDARVRFPITLPKGRAGMQPQL
                     ELTYSQQGGNGPFGLGWGLQVSSISIDTKWGAARYDGTDIYTLDGSQLVPVDNAGNYR
                     TRIEGGFAKIVKQGNYWVVTQKNGVRYLYGQTDNARLQRGSKIGQWLLEWMIDTNGNC
                     IHYTYYHDTGALGAGTPFVQVYLDTIYYTGYNQSNGPFTVQFIRDEGVRKDKQIDGRM
                     GFLVYTRYRYKAIRVSYNGQRIWNYILSYTDGAFDKSLLAKAEVRAGNDSLLYAHDFE
                     YYDEVGWGNEYLNLFGDAVEMENARNVKDDIFIGDYDLGYIGGSKATTNQYQFSGNFS
                     PGSSKKSLAVGAEFSISSTETTTKILFIDIDGDGLADQVYSKGGIKYRRNLMGEGING
                     FADEKPILGLPNLGHDDSTSKTKGVGANAEMAGKRYQKGDSEAKTKSYFSDVNGDGYV
                     DFIDGCLVYYNYGYDEATQSLVFLPTVPEGMVPVSEEIHAPVSPVDEDEEAESKYAFL
                     EYYFRDDPVLAWRAPFNGKISISGEVTVLKPVPASYTTADGVWVSIQKTGTPHSALPK
                     IQGVERGSSKTMALDEIEVKSGEYLFFRVDSGKDGAYDEVQWNPTIRYKGYVPEAVDE
                     NNLYIYQYNVANDFYVYGGGEFEAPVTGKVTIKGGRIGKYAKTSDDIMVTIVKKKITQ
                     TTHITNDGATEDEPDIQILNQYQLPVDAQFIGTNAIPDMINEEVENRETRVYGDDTTV
                     QSVTVTKTIFECTLQSDTPIDYRQVIWENDDGYRGPLLQYTDVDTGTGDNQLSEESIA
                     YIKERIFSLPVATKLFSIPTTYVDNAMQGAINPYVFEGWEVPRAGNYRITMTATNTGG
                     APYKDELVLCVKNRRHGEGFIADCVEKVVNDMQTGLVAGDTLQLQKELSLTEVDDMYV
                     IVTAGKTNGLQLRLKVEYDVLSGNPWVDGDELWVEIPGIEYIVYKAIDPAEIAFGGGF
                     RNWYYGRWNADEYMYKANAPETYSYEFRPMDSSYIKIDAAEAINDPEGFAKKLIVCSS
                     MTPFYKEKVDDTTQSELNEVNPSTTNPPLPVWRGMDDDSWIGPGVFATTRVAKKNIEA
                     IINNSDEGVIDEGFLTQIHTMLASSTGIVDMRGVSKMTKSYNSTHSLGGPGSSVTITT
                     CKSYTKRQFFDINGDRYPDIITDYGLIYTGVNGAIKGWTMGIQGPVQKILSTNYNFGI
                     CPGSNSSQVESEQKGSGKVEKPKQSFPSLSFSLATGDNRSTVDYQDINGDGLPDRIKH
                     SGMLYYAVQYNMGYSLSNDHIIENLYEIQHTKNKSSSLSVGYSTDKSKISGGLSILEN
                     RSEKMDIPLLNDDDRSTLLDINGDGLPDRIVEYNNGLKIYVNTGDGFVEYNKKSIGIM
                     DTTKGRSWTLCFTIGEGIKILPPPLFIGTLIIDGGKSVTKDGNSTTYALRDINGDGLP
                     DIVYQSNNKLYARCNRTGKTNMLKGIKNSIGGSIQLDYQRAGNTHAMPQSQWVLAAVT
                     ADDGTGALDG"
BASE COUNT         4385 a         2456 c         3818 g         3833 t
ORIGIN      
        1 atgatacaac attctatgtg cttgatgaac acaacctgat tgtcaatgga agctattcat
       61 atcatccccc attaaaaaca attgtattta caccagcagg cgggtttgca tggaatgggg
      121 tatatactgc ggtggttacc acaggcatct ataatgcact ggggctccac atgaagcagg
      181 gttatcagtg gtcattcagg attgcgccgt tttatgatac ccagccgcct gcaattgata
      241 caaacagtgt acatcctgct ggtgataatg ttagttgcca ttgcatcata tcgtttcaaa
      301 taattgataa tgggcacatt gatataggaa gtatcaatgg caccaacatc gttgtaagct
      361 ataaaaatgg aagcaatact ctggaggttc cctgcaccta tcagtatcat gcgtcggtta
      421 aagaaatccg gtgcatccct caaagcccgc tgctggaagg gaaactgtat acggtacagg
      481 tacatgaagg gctgcaggat gagtccggca atgcattagc caatccctgc cagtggtcat
      541 ttaccacaga ggttatacct ccgcaggtac tatcggttac accatatgat ggggcatcca
      601 atgtatcggt gggtgagcat atcatcatac agtttagcga gccagtgcag cagagcacct
      661 tgagccaagg gattactgta agcgatagtt atgggaatga tgttgatggc acactcgaat
      721 atgaccccaa tacatatgta gccacgttta ttccgcacca tttagagtat gttactcagt
      781 atacggtaag cgcaggtgag gctataaacg atatatccgg cataccgctg cagccggtct
      841 attccagcac ctttaccacc atgcaggagg atatcagccc tgaaattatt gcaataacgc
      901 ctgatagccc tgttaatacc gatagcacct tccagatttt gttcagtgaa tccatggacg
      961 aagcatcgct ggttgaacat attggtgtaa gagatagtta tgggaatgaa atcccggtac
     1021 atattaatta tgatgatgca atatatcttt gcacggtaac tccggatagg ttgattggtt
     1081 tttataacta tacagtatat atcaccaccg gtgtcacgga tatccatggc aatccgctaa
     1141 tccataatta tgaaaaactg tataccacca atccggcgcc ggtaaacacg gtggtcatgc
     1201 tgtatgctcc ggttgattat gaaggcgggg actatataga tggtgatatt gatgaattga
     1261 ttgatgcaca ggtagatcat tcattggtaa ggatagtggc tctggtggat tatccctatg
     1321 atgggaatac tgtcatcgtt gaaagtatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnntg cccgttttga cagcggtttt tattatccca tcccatggta tgttgattgg
     1501 atagattttt taaacaaagc aaatgctggc ggattactta taggtaattt taaccggatg
     1561 gtttgtaaaa cagttatggc gtccggacat caggggtatg gtattggcat catgtttcca
     1621 acaaagacgc ctgaagattg gtataacaat attaatccgt atggcattga ttttattggc
     1681 gattatggat gggatgagtt tttgcaggga aaacattttg gactctatgc agatagcaat
     1741 gagccattga atgatgatgg ttttagcgca atgaccatca gcagtacaga cgtgacaagg
     1801 caggctatct attataatta tattcatgat ccagatgata cagatatata tacggtgaca
     1861 gtaaatcaac atgcaggtgc aggtaatgca gttacatcgt cagatgaaat accaataaac
     1921 aatgaaaaaa taaaatggca gacggtatcc tgctcgtatg atggggacgt tattgctgca
     1981 acagcacaaa acaggtatat agacccattg caggatgccg gcacgccatg gctacaaaga
     2041 acaggcacgg ttgcacaaaa ttggagcaga acacaaacat cagcccttga atcagcagta
     2101 gccgaagttg ttgatggcac ccttactatt gctgtaacgg gtatcccgga aaactgtgag
     2161 cttgcaatga ttatcaagga tcacaatggc accataatca gggaagccat caggagcgga
     2221 ttgggtggca ctttgcgatg tgatttacgc atatacaccg gcgtgtatgt tcaataccgt
     2281 gtttaccttg ttccggtgaa tatcatggga tggtcaatga atacccatga acactatacc
     2341 atacaattga gttatcaaaa gaatgaatag cgatcatgga gaaacaatgg aaaataaaaa
     2401 caaaagaaag attctcataa acaaatatta tcagttgaaa acatcgtttg gtgtggcagg
     2461 gctttttgca ctaagcatgg gtattctttt tatcataagc agcagcatgc ttgtatggaa
     2521 taacatatct attgcaaagg ctatgacaca tcagatggat ctgcaaaagg tgcaggagga
     2581 tatcttacag tctattgaaa tgttagcaaa atatggcaac aaaaaaaatc ttatctttga
     2641 ggcatataag gcaattggtg atctaaagag aaatactgat gccctgaagg aaagcaatga
     2701 gcttttgcgc aggagcattg ctgttaatca ataccttatg tgggtaaatg ggatattggg
     2761 agtggcgctc atcatagcgg tatttgttat tgtattacgg cgtacacacc gtgtggcggg
     2821 tcccatgttt ttaatgaaac gatatatgaa tgacattata caggggagag tacccgaagt
     2881 aagggcattg aggaaagatg atgagtttaa ggatgttttt gaaacctttg ttagcatgat
     2941 tgaaacattg catttaaatc gcttaaaaag gttccagaaa agcaaaacaa agagcagcaa
     3001 gggttaatgg tacaccatgg tttggtacag ttgccagtta cagtggaagg taaatcccag
     3061 ggtgtacatg aagtatgaac tgtgcgagga ggattcaaag taggttgtat atgcagcgct
     3121 gcttgataat gacagttgtg ggtcaagcat aagcgcaagg atgatgcccg ctgcaatctg
     3181 ggggttgtaa tagatggatt gtgaatacga atatggcgga tagtagtgat gggtgtcgcc
     3241 gctgattatg tgcatggtat atccggcgcc tatgaaaggg gtacatgaca atgatagcgg
     3301 aagcttaata cggtaaccgg cctgcatcat gaagcttatg ctgtgccagt agttaacggt
     3361 gacaacaggg tcatggagca taaaccatgt taatgcaggg ataacaacaa gtgatgggag
     3421 ctttgtattt tcaatacagg ttgatgcctg gaagccggaa ccggagtgtg catagtcttt
     3481 aagctctttg aaagcatcaa tatacacggg agacagggta atggcggtaa gcgttatggc
     3541 aaatgctgct tggtacaaaa cggggatgcg cctggacgag taaaagtcaa tgatgtcatg
     3601 ggcgatagtt acatacaatt gttctaattg ttgtgatgta aggttttcct tttgtatcac
     3661 atattcctgc gggtctgtac cggatgaaac cgttaccgtg atggagtagc tggtttgttc
     3721 aattttttct ttgaggtaag gggtgagttc cttatacagg attgattgct gttcagtaat
     3781 ctcgacggta agtgtacgga tgagctcacc tgctggcggg atattaatgg caccggcagg
     3841 gattacatat gaggagctat cgctaataaa tacgccggaa gacagcagtt gttgtataag
     3901 gttatgatgc agcacggtta ccgtttccga tggctggata cagtgattgc acacgggcgg
     3961 tttgctctga agtataaagg ggtagtagct ggcataggct ttatatggca agagcataca
     4021 aaacaatagc agcacaataa atgtgtatcg aggtttatac atgataattt ataaaaatcg
     4081 tttttctggt tattatgata aacaattgat tttaatcaag aatattttca taattatttt
     4141 atttttttta ataattatta tttgctcatt atatattgca aaaatgttat attactttta
     4201 atctattggt gcttgaatgt tctggtttta tggaaaaaat gtacatgcaa tacagttgtt
     4261 gatgtatagc aggatacgca gcgcaggtgt acgggtagcc acggtgctta tgctggtagt
     4321 tttactgcac tgctacggct gtactgatgt taattaccca actgacgttc tggataaaag
     4381 cccctatgat atgagcggtg gcagttttgt cattgatggc gatgcgccgt ttgcctattc
     4441 gcaacaggtt atgctgagca gtcacgttga taatgcgtac tggatgcaat tttcaaatga
     4501 tgcaatacgc tgggtggact gggttgcttt tgataaccat tctatgtggt tgttgccatt
     4561 gcagtacggc gaaactactg tgtatgcaca ctataaaaat atacgggggg acattgtggc
     4621 gtaccaggat tccatttttt ttgtagagcg gctggtaaac aataccagca tacagcttgg
     4681 cggtgatgtt gctgtatcaa atgatggagc tattgttgct gccgggacgc tgtatggaag
     4741 ggcaaatgca gtgtatgttt ttcataagca gagcatagac tggcagggta caaagattaa
     4801 acctgatgac attgtggatg gcgataggtt tggttattgt gtagcgctga gcggtgatgg
     4861 cagatggctt tttgcgggag cgccgggcaa aaatgctgtg tatgtgtatg annnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttggggata tactccccgg tgatggacaa
     5041 cactatgctt tttttggaca gtcggttgct gttactgatg atgcatgctg gtgtgttata
     5101 ggctcgccag gctatgacat acttcccaat gatgataaag gctgctttta tatatttaaa
     5161 aggaacgctg acaatacctt tttattactg acccagataa aaaatacagg cggcagcagg
     5221 ggtgatgcac tgggtggtgc tgttgcaatg tcaagagatg gaaggactat cgttgcatcc
     5281 atgcacctgt atgatactgg tcttaatgcg gttgatagag ggttggtgca ggtatatagc
     5341 tttgatggca gcggtgttga acacagtgat acctattccc ctgatgacag ggatacaaca
     5401 tgttattttg gcaatgctat tgcagcaagc aataacggtg tgatagtcat tggtgcgccg
     5461 tatgcatata gagatacggc aggtgataat ggcggggttg tatatatacg gcggtggctg
     5521 tgaccattgg taaacaggta ttgctcaagg atacagggag aaagtaatga gaagattggt
     5581 gatgatgcta ttgattatgg gtatggcggg cagtgtatca gcacaggaat tgcagcagaa
     5641 ggatgaaatg aaaaaacaag caccattggt gaagacattt tttgcgatgg caggcacaat
     5701 gtacggattt gcgtacccag caggaactat cgatgctatc aatggacatg ttgagcgtga
     5761 aaaggacaca tattttggtg gcaacattgg aattggctat caactatcac cactattctt
     5821 aatccatggt ggatttactc tgtgggatgc tgaggtgagt gtgaagcgca atttactggg
     5881 gcagaaactg gaaacgcgtt attgtctgga tacctgtgct ttgcatgccg gaattatgat
     5941 ggtgtataaa tggttttatg ctgactgtat gctttttacc gaattgcctc tggaggatga
     6001 taccgagaag gtatatctat caggagaata tatgcagacg aacaggttaa agtcgcgcta
     6061 tgaatacgga ggctttttag ggctgggtgt caggataccg gttatggagc agttttttgt
     6121 attagccggt atctatggcg ggggaggatt tgttccctct cttgaaagct cacggggtga
     6181 tgaattgtat ttaatcaacg ccacaatcag gataagtgtg atggtgtatg tgctgtagga
     6241 agcgccactg attcatcatt ggtattgctg ggagtgggat gacaatgaat aagaggaatg
     6301 gtcatgaagg tgtataaagg gatacttttg attttgttgt ggagtgtata tacaggttgt
     6361 agcaccgcag atacagaaag tttatcaatt gaagggcagg tgatgctatg cggtgaaggg
     6421 atggagggtg tatgtgtggc gctgcaacat ccgcaatacg ggagtgtgga aacctacact
     6481 gatgatctgg ggatgtatca ttttaaagaa gcatgggcag gtgattatag tgttaccccc
     6541 tttgtggatg gctgtgagat agttccggta aaacgagagg tgagtgtatt tgataatacc
     6601 gtggtaaatg attttattat aaggccattg tgggaaagga cgtatggcag tgattatcag
     6661 gatgttgtga atgccatgac gaatacacgc aactgcgggt atgtgtatgc tggcagtacc
     6721 gacttacatg gcagtcatga ggcttttgca atacggctca atgcaatggg taccatgatg
     6781 tggtacaaca cctacgggcg ggatgtgtac cacagtgcca atgctgtgct ggcactggat
     6841 gatggaggat gtatgattgc tggaagcaaa ggcagcagtc aaaccaatgt ggatatatgg
     6901 gtggctaaaa tagatggcaa tggtgcttta caatggcaaa ggacctatgg aggaaatcaa
     6961 tatgatgaag cccgtagcat agtaaacgta aatgatgggt attgtattgc aggatgtaca
     7021 gagtcgtttg gtaatggcaa ggaggatgta tatgtcatta aaatcaatga tgatggcact
     7081 gttatagatt ctacttactt tggcggggta aatgatgata gagcattaaa gattataaag
     7141 tgtaaaaacg atagcggatt ccttattgcc ggaataaccg aatatgaaaa tcagcttgtt
     7201 gcagatagct gggttgtaaa gcttaatcct gaccttgagc tgtcgcccaa tggatggacc
     7261 aaagagtacg ctattgcgtt atatgactgt gctaccgata tactggagct tgatgatggc
     7321 tcaattattg tatgtgggtg gggatgggat gttgaaaatg ccagcaggat atgggtttca
     7381 aaattacgag gggatggatc ggttgaagaa tggacaaagg aatattatat tggcgcaagc
     7441 tgtgcggtta acagcatgat acagcgtgca gatggcagca ttgttttaac cggatacaca
     7501 atgactgcgt taaatgatga aaaggatata ctgctgttag cactggataa tgaagggaat
     7561 gtgctgtgga cgcaggtata tggtagcggt aatgaaatta atgatgaagg caaagccatg
     7621 gtattggctg gcgatggggg gatttcggtt gcttgtgata tgaaagtatc tggcgttgcc
     7681 ggcgatgtac attgccttaa ggtaaacgat aagggattgc tcaggtaggt gtatcacatt
     7741 agatgggatt cgggagcagg tccggaatga cggtgtggcg taattctatt tttggagaag
     7801 taaaacagtt tgaagaagca agattacaac aatagcacaa tatattttgt aactgttaca
     7861 acaaagggca gagtttgcct gtttggtact attgacgagg ggcagatgca tttaaatgaa
     7921 atgggaataa ttgtgaataa ttgctggttg gatatccctg aaaattttcc cgatgccgtt
     7981 atggatgttt ttgtggttat gccaaatcat gtccatggaa tcattatggt aaacgatagt
     8041 cagaagaaaa caaaaggcgc taagaaaagt atacacaagc ctgaaaagag attacttgaa
     8101 gcaattattg ggcaatttag ctataaatcg acaatagaaa ttttaacgat gtcacgccgt
     8161 gaaaaggcag atgaatggag atggagtatc tgggatgata attattacac aaggattatt
     8221 gagagtgaac attatttaaa tatcttacgc tggtatataa ttcagaatcc ctatcgatgg
     8281 gatgttgata gagaaaatcc atatagaaac atacaaaaac ggagggatat gccatgaaga
     8341 aggcatgctt tttattattg ttgtgtacaa tactattact atcctgcagt ggcagtaaac
     8401 ataaaaatga agcgataatc aatgggatag atattcctgt gcggatatat cctgccggtg
     8461 taacagtagc aggtgaggat ggcagagagc aaccgtccgg cgatagcatc cttgcgtatg
     8521 acaccagcat taccagtaca tggcatgtac tggaaaaaag cgtcataaca tttcaatgca
     8581 atcataccta tattgatacc atacaattat atgatgctgg tggcgttaca ctaaaactat
     8641 atggcgacag cgctgatggc agactgctgg caaccattga tgatactact gtacgcaatg
     8701 atggctggat tacggtgagc attggcaggg aggtggatgc gctggtgctg gtggtggagc
     8761 ctgctggcag cgatgctcat attgctcaca ttgagctgtg gggaagtgct gatagcgttg
     8821 cggggatact gttgcactat cgcaatgtac cggcgcttga cgaaaccagt gttgatgaaa
     8881 cggttgccga tatctacccg acactgctga aagctggtga aaacagctat gaggcaacag
     8941 tcaatattac aaaccctgca atgtaccggg cagtatacgt ggtgtacacc ggcagaggga
     9001 tataccgtcc cgtaagtgtg atgatgaagg ttaacgaatg tgactggctg ggagggctaa
     9061 ccatccccga atcagcaggg caggaaacca ttcaacggca gcaaagctgt gagataaacc
     9121 cattgtggct taccagtggc agcaataccc tgcatttcaa aggggtaacc aatgaggttt
     9181 cccttcagga ggttacggta gttggcatta aggataccgg ctataacgag ccacggcagg
     9241 gtacagaggt actcaccgat ggcaatggca tgacaggtgt accggcaacg ggcggggcga
     9301 tagtaaccta taatcgtttg atgcgtatgt atggagtgct tgtgtatgca ggggacaatg
     9361 ctggcagcat acaggtagtg tgcaaaggag gcgcaggcat atcagaggga gggatgtgta
     9421 ctgcaacaca ggcaggctgg tactatatac cattgaacaa gcccgtaacc ggcaatgaga
     9481 ttgcggtaac ccttgccaat actgcctgcg tgataggcga ggtggctgcc tgcgcctcag
     9541 cggctgaagg tgctcatcct gcaattgtgg taagctatcc ccggaacgga gaatattttg
     9601 gcaggactgc ctatatacag ggatttataa cgccgtatgc taccgatgat ggggtggnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca gggcttaccg taacggttag
     9781 tcctgacagt gctaaaagca tatactatca gggagtgcag atagaaatac cagaaaaagc
     9841 ggtggacgaa gatgttacca ttactgttgt accgctggat gagcagcaga tacaaaacct
     9901 tacaccgggt atgcacaatg tgaccgcccc tgcagcggga tacagatttt tatttaatgg
     9961 aaaggcacat ggaagtttta aaaagcccgt aaccataagc attccgtatg ataagcacaa
    10021 gctgttgagc ggtagtggag aacatgagat acggatgttt tattatgatg acgggatggg
    10081 tgtgtggaag cagatggagg caagccgtgt ggtaacggca aagagtgcag actatatcct
    10141 gtatagcagg aatataacaa gcacacaggc aggtggctat gtggtggtag agtccaccca
    10201 ctttaccgat tttatcaatg ctaccttaac agtgccggag caccctgagg cactgcagtt
    10261 taatcccaac agtataaagg atattaaagc aggagagcct ttagcaggaa tgcagcttat
    10321 tcagccgcct cagagcagca ataccggtga tgcgcgagtg cggtttccca ttacccttcc
    10381 taaagggagg gcagggatgc agccgcagct tgagcttacc tacagtcagc agggaggcaa
    10441 cggacccttt ggattagggt ggggcttaca ggtatcatca attagcattg atacaaagtg
    10501 gggagcggca cgctacgatg gcaccgatat ctatacgctg gatggaagcc aattggtacc
    10561 ggtggacaat gcagggaact atcgcacacg catagaagga ggctttgcaa agattgtaaa
    10621 gcaggggaat tactgggtgg taacgcagaa aaatggggtg cgctacctgt atggtcaaac
    10681 cgacaatgca cgattgcagc gaggaagcaa aattgggcaa tggctgttag agtggatgat
    10741 tgataccaat ggcaactgca tacactatac gtactaccat gatacgggag cactgggggc
    10801 aggtacgccg tttgtgcagg tgtaccttga taccatttac tatacagggt ataaccagag
    10861 caatgggcca tttaccgtac agtttatacg ggatgaaggc gtcagaaaag acaaacagat
    10921 agacgggagg atgggttttt tagtatatac ccgctaccgc tacaaagcaa tacgcgtaag
    10981 ctataatgga cagcgtatct ggaattatat attgagctat accgatggag cgtttgacaa
    11041 aagcctgctt gcaaaggcag aggtacgggc aggcaatgac agcctgttat atgcgcacga
    11101 ttttgagtac tatgatgagg taggctgggg aaatgaatac cttaatttgt ttggcgatgc
    11161 ggttgagatg gagaatgcaa ggaatgtaaa agacgatata tttataggcg attatgacct
    11221 tggatatatt ggtggatcaa aggcaacaac aaatcaatat caattttcgg gaaatttttc
    11281 tccaggcagt tcaaaaaaat cacttgcagt gggtgctgaa tttagcattt caagcacaga
    11341 aaccacaacc aaaatactgt ttattgatat tgatggcgat gggctggcgg atcaggtata
    11401 ttcaaaaggt gggataaagt acaggcgtaa tttgatgggt gaaggcatta atggatttgc
    11461 tgatgaaaaa cctattcttg ggttacccaa tttaggacat gatgatagca catcaaagac
    11521 aaaaggagtg ggtgctaatg ctgagatggc gggcaaaagg tatcaaaaag gggactcaga
    11581 agcaaaaaca aaaagctatt tttcagatgt caatggagac ggctatgttg attttattga
    11641 tggctgtttg gtgtactaca attatggcta tgatgaagct acacaatcat tggtgttttt
    11701 accaacagtt cctgaaggga tggtaccggt atcagaggaa atacatgcac cggtatcacc
    11761 ggttgatgag gacgaggagg ctgagagcaa gtatgcattt ttagaatatt atttcaggga
    11821 tgatccggta ctggcatggc gtgcgccgtt taatggtaaa atatcgataa gcggagaagt
    11881 gacggtatta aaaccagtac cggcaagcta tacaactgct gatggggtat gggtaagtat
    11941 acaaaaaaca ggtacgcccc attctgcatt gccaaaaata cagggtgtag agcgggggag
    12001 cagtaagacg atggcacttg atgagataga ggtaaaaagt ggtgaatacc tgtttttcag
    12061 agtcgattcc ggtaaggatg gtgcctatga tgaggtgcag tggaatccta ccatcaggta
    12121 taaggggtat gtgcctgaag cggttgatga aaataattta tatatctatc aatacaatgt
    12181 agcaaatgat ttttatgtgt atggcggagg agaatttgaa gcaccggtaa cagggaaggt
    12241 aacaattaaa gggggaagga taggtaagta tgcaaagaca tcagatgata taatggtgac
    12301 gattgtaaag aaaaagataa cgcagacaac acatattacc aatgatggag caacagaaga
    12361 tgaacctgac atccaaatac taaatcagta tcaattacct gttgatgctc agtttattgg
    12421 taccaatgct attcctgaca tgattaatga agaggtggag aatagagaaa cacgggtata
    12481 tggggatgat accactgtac aatcggtaac agtaacaaaa acaatttttg aatgtacttt
    12541 acaaagtgat acacctattg attaccgtca ggttatatgg gaaaacgatg atgggtacag
    12601 gggaccatta ttacagtata ctgatgttga tacaggcacg ggggataatc aattatcaga
    12661 ggaaagtatt gcatacatta aggaacggat attcagcctg ccggtggcaa ccaagctgtt
    12721 tagcataccc acaacatatg ttgataatgc catgcagggt gctatcaatc cgtatgtatt
    12781 tgaaggatgg gaagtaccgc gtgcaggtaa ctaccggata accatgaccg caaccaatac
    12841 tggcggtgcg ccctacaaag acgaacttgt actgtgtgtt aagaatcgca ggcatggtga
    12901 aggatttata gcggattgtg tagaaaaggt ggtcaatgat atgcaaacag ggcttgtagc
    12961 cggtgataca ttacaacttc aaaaggaact atcgcttact gaagttgatg atatgtatgt
    13021 cattgtaacg gcaggcaaaa ccaatggttt gcaattgcgt ctgaaggtgg aatacgatgt
    13081 gttgagcggc aatccatggg tggatggtga tgagctatgg gtggagattc ccggcataga
    13141 atatatagtg tataaagcca ttgatcctgc agaaatagca tttggagggg gcttcagaaa
    13201 ctggtactat gggagatgga atgctgatga gtatatgtat aaggcaaatg cgcccgaaac
    13261 atacagctat gaatttagac ccatggatag ctcttatata aaaatagatg cagcagaagc
    13321 aataaatgat cctgaagggt ttgcaaaaaa attgatagta tgttcttcaa tgacgccatt
    13381 ttacaaagaa aaagtggatg ataccacaca aagtgaactg aatgaggtaa acccatcaac
    13441 gacaaatcct ccattgccgg tatggcgtgg gatggatgat gatagctgga taggtcccgg
    13501 tgtgtttgca accacacggg tggcaaagaa aaatatagag gcgattataa acaacagcga
    13561 tgagggtgta attgatgaag gatttttaac gcagatacac accatgcttg caagcagtac
    13621 cggcattgta gatatgcgag gcgtatcaaa gatgacaaag agttacaatt ctacacatag
    13681 tttaggcgga cctggctcat cagtaactat cacaacatgt aaaagctata ccaaacgaca
    13741 gttttttgat atcaatggtg ataggtaccc tgatattatt actgattatg gacttattta
    13801 tacgggcgtt aacggtgcta taaaaggatg gacaatgggc atacagggtc cggtacaaaa
    13861 gatattaagc acaaactata attttggtat atgccctgga tcaaattcaa gccaggtgga
    13921 atcagaacaa aaagggtccg gtaaagttga aaaacccaag caatccttcc caagcctttc
    13981 cttttcgtta gcgacaggag acaaccgtag caccgttgat tatcaggata tcaatggtga
    14041 tggtttgccc gacagaatta agcacagtgg aatgttatat tatgctgtac agtataatat
    14101 gggatacagt ttaagtaatg atcatattat tgaaaatctt tatgaaattc aacatacaaa
    14161 aaacaagagc agttcattga gtgttggata tagtacagat aaatcaaaaa taagtggtgg
    14221 actatctata ctggaaaata gaagcgaaaa aatggatatc cctcttctta atgatgatga
    14281 taggagtaca ctgcttgaca tcaacggtga tggattgcct gacaggattg ttgagtacaa
    14341 taatggattg aagatatatg ttaatacagg tgatggattt gttgaataca ataaaaagag
    14401 cattggcatt atggatacta caaaagggag atcgtggaca ctatgtttta ctattgggga
    14461 aggaataaaa atattacctc ctccgctttt tattgggacg ttgataatag atgggggaaa
    14521 aagtgtaaca aaagacggca attccaccac ctatgcactt cgcgacatca atggagacgg
    14581 attacccgac attgtgtatc agagcaacaa caagctatat gcacggtgca atcgcacggg
    14641 gaaaaccaac atgctgaagg gaatcaaaaa cagcataggg gggagcatac agcttgatta
    14701 ccagcgagcg ggcaacaccc atgccatgcc gcaaagtcag tgggtgcttg cagcggtaac
    14761 ggctgatgac ggcactggcg cacttgatgg gg
//
LOCUS       DAOOJH010000058        14775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3901_length_14775_cov_12.121332, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000058 DAOOJH010000000
VERSION     DAOOJH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3901_length_14775_cov_12.121332"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLJ75_08360"
     CDS             <1..298
                     /locus_tag="PLJ75_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05264.1"
                     /translation="NERYKDIIVQFAHDAFHYLLWLLWYIAFNPVRKRQCDSPVKYRY
                     SSIQSYVQEGADVGVPIDHHEYFLQLGDSFSERVKRFLQYEEAYRKRYSIAQWV"
     gene            complement(413..2110)
                     /locus_tag="PLJ75_08365"
     CDS             complement(413..2110)
                     /locus_tag="PLJ75_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD05265.1"
                     /translation="MILLNPKKYSAQFSDKESEEIMRKTIDFFETKGLKKILDDDHNY
                     VWYDDFLQFQKDNQIFAKFFTPEKYGDKNSRWDTYRIVNYAEILGFYGLCYWYTFQVS
                     ALGLGPIWIGKNEEVKQKAAKLLKEGAIFAFGLSEKEHGADIYSSDMMLYPNGDGTYR
                     ANGDKYYIGNANKAAMVSTFGKFADTGDYVFFVADSQHEKFECIKNVIQNQSYVAEYA
                     LHDYPIAEADILDKGRDAWDSSLNTINICKFNLGWGSIGIATHCFYEALNHAGHRNLY
                     GKYVTDFPHIKQFFVDAYTRLVAMKLFALRATDYMRCANENDKRYLLYNPMVKMKVTT
                     QGEEVINLLWDIIAAKGFEKDTYFHMAARDIRALPKLEGTVHVNMALIIKFMFNYFFN
                     PKEFPEVPQRLDAANDDFLFNQGPTKGLSKIQFHDYNIAYNSVNLPNVEIFKKQINAF
                     KDFMTGATPDKQQSEDIDFLLSIGELFTLVAYGQLILENKTIYAVEDDLIDQIFDFMI
                     RDFSKFALQIYSKTSSTSKQQELCLKMIEKPVVDHDRYNRVLEKYVYALKDEYYMNE"
     gene            complement(2489..3559)
                     /locus_tag="PLJ75_08370"
     CDS             complement(2489..3559)
                     /locus_tag="PLJ75_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPD05266.1"
                     /translation="MKNICTIQAGAKAYAIIKDEGIQKERIRCMLGAAGGPKWIILYG
                     LDKALYKSFLQKRKNPVYMIGSSIATWRFAALSLADGLQGIEAFKERYFAQKYSMKPD
                     REEITAETVAILEEFLPDSKVDEIVKHPTQRLCIITARSRHLLQYEDTFRLGAGLGIN
                     AITNMVSRKLVGLHFDRFAFYDAREFPSFIQWKNYHTQYIPLNNNNLKKAVLASGSIP
                     LVMNGVVFQHEGKTHILRDGGLVDYQQDLPLEPGDYIALYPHYSDRIIPGWFDKPLKY
                     RKPHHALENVCMVSPSKEAIASLRYKKIPDRKDFYTFAGDDSRRLLFWNETVALGQQI
                     AEEFMELVESGRIKDRVIPLAR"
     gene            complement(4046..4849)
                     /locus_tag="PLJ75_08375"
     CDS             complement(4046..4849)
                     /locus_tag="PLJ75_08375"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPD05267.1"
                     /translation="MITYIAILFVYEDTAVSQEKAVKQIYLSSNLKKKGLVEVREGIA
                     FVKIPAGSFMQGGNKVKVNGFWMAMCEVTQQQYESIMGINPSYFKGNPDNPVERVNWY
                     NAVEFCNKLSIKAGCKPYYTIDKVHKDPENKNTGDTIKWTVTINGGNGFRLPESAEWE
                     YACRAGTTTRYYWGDNVNGDYCWYNANSDEQTHPVGTKLPNAWGLYDMSGNVWEWCFD
                     WDIDDYGVFRIIRGGSWNYDADLMESSVCNYGYPNDTFRILGFRPVRNL"
     gene            complement(5156..5986)
                     /locus_tag="PLJ75_08380"
     CDS             complement(5156..5986)
                     /locus_tag="PLJ75_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD05268.1"
                     /translation="MAIKNYVISVLLIVSVGLLMLACKKEAKEKVVVKGSTTVLPIAQ
                     RSAEVFYEKNKVVISLSGTGSGDGIKALIDGSCDIANSSREMHEVELEMAKRKGETIK
                     GVAIAYDMIVPIVHPSNPVGNLTLHQLKAIYEGSVKNWNDVGGNDETIVVISRDSSSG
                     TYEVWESKVMKNVEVRKDALLQASNGAVLSVVARNKKAIGYVGYGYLNDTVKVLFVNS
                     VEPTIPNGKSGKYPIARKLYMYVNDVKIKPAAQNFIDFILSPEGQSIVKETGFIPIID
                     "
     gene            complement(6291..7118)
                     /locus_tag="PLJ75_08385"
     CDS             complement(6291..7118)
                     /locus_tag="PLJ75_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPD05269.1"
                     /translation="MKVKSFLVLMITAFVFSVSMGFSKGNDAKVVIKGSTTVLPIAQK
                     AVEVFYDKTKIIISLSGTGSGDGIKSLIDGSCDIANSSREMKPEELAMAKEKGEKIKE
                     VAIAYDMIVPIVHPSNPVKNLSLEQLKAIYEGTITNWKEVGGNNEKIVVVSRDSSSGT
                     FEVWESKVMKKAEVRKDALLQASNGAVLSVVARNKKAIGYVGYGYLDKTVKGIYVNNV
                     EPTIPNGKSGKYPIARKLYMYVNDVKIKPEAKQFINFILSNEGQIIVKEAGFIPIID"
     gene            7684..8448
                     /gene="pstB"
                     /locus_tag="PLJ75_08390"
     CDS             7684..8448
                     /gene="pstB"
                     /locus_tag="PLJ75_08390"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPD05270.1"
                     /translation="MSEKEIKVQAKNLSFYYGSSQALFDIDLTVLANSVMALIGPSGC
                     GKSTFLRCINRMNDIIPYTHIAGDMTIDGESIYTIKYDVTQLRRRVGMVFQKSNPFPK
                     SIFDNIAYGLKINGIKDRYIIEKTVEDSLKRTALWDEVKDRLNDSAMSLSGGQQQRLC
                     IARTIAVQPEIILMDEPASALDPISTQKIEELIQDLKKQYTIIIVTHNMQQAARVSDY
                     TAFFYMGKLIEVNKTEVMFTKPDKQMTENYITGKFG"
     gene            8473..9126
                     /gene="phoU"
                     /locus_tag="PLJ75_08395"
     CDS             8473..9126
                     /gene="phoU"
                     /locus_tag="PLJ75_08395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPD05271.1"
                     /translation="MSTHLEQELNELIRKILEMADIAIEAVEDSVQSLKNIDVKLAEK
                     VIQKDTALDTLEVFIDEECVRILVTKQPAAADLRLILALFKSNTDLERIGDFATNIAK
                     ETIRLEGKSLLKPLIDIPRMEKICIDMIKLAFRAITEKNIDYAKRCIDMDKEVDELNM
                     QINRELFSFMVENPATISPAFGLITVARALERIGDHATNIAERAVYYIQGEDIRHQE"
     gene            9130..9813
                     /locus_tag="PLJ75_08400"
     CDS             9130..9813
                     /locus_tag="PLJ75_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD05272.1"
                     /translation="MSKIYVVDDEKDIREILKVNLQKNGYDVNTFASAEEVLKQLAIA
                     KPDLFILDIMMNGMDGLDLCKHIRASRELTTIPILFLSAKSAELDKVLGLELGADDYL
                     TKPFSIHELIARVKAIMRRSQTKQELSPKEILTYNAISLYPDKFQVTIDNQNIDLTKT
                     EFLILKLFMQYPGKIFSRDNIIDSIRGDNVYVIDRTIDVHIMNLRKKLGTYKDAIKTY
                     SGVGYGLKV"
     gene            9842..11578
                     /locus_tag="PLJ75_08405"
     CDS             9842..11578
                     /locus_tag="PLJ75_08405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05273.1"
                     /translation="MKRLKTKLVVVYTVALVIFISFLLMYINHHIHSAFIAFQEAELQ
                     QNIENIHIYLHSYHKPFNYVLFQNHDVVASLKNVSYKSSLRITIITDDGTVYFDSFAN
                     PELMENHLYRPEILQSRTKGVGSAIRYSKTVKSDMLYVAKYFDNYYIRTAKSLGAINI
                     LIGALTKNILIVGFAVLVLTIAANYIFATIFTKPIAESLQFIDQFFHGDLNTRILNYK
                     DDEMGYLQNSMNRLADNIQARINELTAQKNKLFMIIESIHDPIALIDDSKKITVYNKA
                     FIELFQFPETVTNTYYFSIIRHSQLNAKIHVALSQRQNFSFEETIGSNHFQIFIYPFP
                     QPDSGILLLMHDVTEQKRIQKLKSDLVGNLSHELKTPISIIRGYLETIKDISLDEKTA
                     GQFIDNALANLERQNAIINDMLKLNELETTAYAVTEYIDIKTIITRCIELLTPKIQKK
                     NLAIVMDIDAIPETIAGNAFLTEEIFFNIIDNAINYNNPNGAITIKSAVKTATISISI
                     ADTGVGIPQDEIPRIFERFYRVDKSRSRDTGGTGLGLSIVKHAAQILGWNVSVTSSMN
                     GTVFAITIPWAG"
     gene            complement(11657..11854)
                     /locus_tag="PLJ75_08410"
     CDS             complement(11657..11854)
                     /locus_tag="PLJ75_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05274.1"
                     /translation="MRASRYLSEKEMLQKGIDVLLDALGPVETVRFLNISRDKRIESV
                     KRHRMWQKSLKKDDFINEIVK"
     gene            complement(12083..12301)
                     /locus_tag="PLJ75_08415"
     CDS             complement(12083..12301)
                     /locus_tag="PLJ75_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006527552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05275.1"
                     /translation="MKNANKIYMDVCSLCRPFDGQHYLRIRLETNAVNLILSKVKDSH
                     LELINSPVLIKEIESIDDDLERTELCNY"
     gene            complement(12402..13268)
                     /gene="pstA"
                     /locus_tag="PLJ75_08420"
     CDS             complement(12402..13268)
                     /gene="pstA"
                     /locus_tag="PLJ75_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPD05276.1"
                     /translation="MATLQQKAKYYETSSFVIITILSYAIIGFLLFVLGYIFLQGYKA
                     LSWDFIVSFPKNEMREGGIFPAIIGTLYLMIGSSLVSVPIGIMTAIYLTEYVENVTVL
                     NFIRLGINNLAGVPSVVFGLFGMSVFVIFLDFGSSIIAGSLTLGILNLPLVIRSTEEA
                     LLTVPMSYREASLSLGATRWQTIYKIVVPTALPGILTGIMLSLGRAAGETAPIMFTCA
                     TFYTPFVPDSLFSEVMALPYHIYVLATAGTHIEATRPIQYGTAIVLIVLVLGLNLAGM
                     IMRYSYRKKLKG"
     gene            complement(13273..14181)
                     /gene="pstC"
                     /locus_tag="PLJ75_08425"
     CDS             complement(13273..14181)
                     /gene="pstC"
                     /locus_tag="PLJ75_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPD05277.1"
                     /translation="MLKERLKIALNIHTASDRIIRYIFAVVGFVSVAFLFLIMIFLFK
                     EGLPVFNSVSVKEFLFGTSWYPTSVPPKFGTLPLIVASLTVTFFAAAFAVPFSMAIAI
                     YLSELAPATLREIVKPIIELIASIPSVIIGFFGMVVVAPFLQDHFDIDTGLNVLNAAI
                     MLAFMAIPTIASISEDALSSVPASLKEASYALGANRWQTIVHITMPAALSGVWTAIIL
                     GISRVLGETMVVLMVAGGATIFPTSLFDPVRPLTANIAAEMAEASYRSDHYFALFAIG
                     IILFVLTLLFNVIANYLSMKYKFKWN"
     gene            complement(14314..14775)
                     /locus_tag="PLJ75_08430"
                     /pseudo
     CDS             complement(14314..14775)
                     /locus_tag="PLJ75_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673847.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT         4741 a         3024 c         2444 g         4566 t
ORIGIN      
        1 gaatgagcgc tataaggata tcattgtaca gtttgcccat gatgcgtttc attatctgtt
       61 gtggttattg tggtatatag cgttcaatcc agtgcggaag cgccagtgcg atagtcctgt
      121 caagtatcgc tacagcagca tacaatccta tgtacaggag ggtgctgatg ttggtgtacc
      181 gatagaccat catgagtact ttctgcagtt gggggacagt ttcagcgaac gagtgaagcg
      241 gtttttgcag tatgaggagg cgtaccgcaa gcgttattct attgcacagt gggtataaaa
      301 aaggggtcag agcctatttt aacgaatacc aaaaaagagg ccgtctttat tagagacagc
      361 cccactttta cgctttaaca cccgtataca acaaattcaa ttactgtatt atttactcat
      421 tcatataata ttcatccttg agagcataca catatttctc cagaacgcgg ttatacctat
      481 cgtggtcaac aacaggcttc tcaatcatct tcaagcacaa ttcctgttgt tttgatgtac
      541 tgcttgtttt tgaataaatt tgcagagcaa actttgaaaa gtcacggatc ataaagtcaa
      601 atatctgatc aatcaaatca tcttccaccg catagattgt cttgttttca aggataagct
      661 gaccataggc aaccagcgta aacaactcgc ctatagacaa taaaaagtca atatcctcac
      721 tctgctgttt atcaggggta gcaccggtca taaaatcctt aaacgcatta atctgttttt
      781 tgaaaatttc tacgtttgga aggttcacac tgttataggc aatattgtaa tcatgaaatt
      841 gaatcttact caaccccttc gttggaccct gattaaatag aaaatcatca ttggcagcat
      901 caagacgctg aggaacttca gggaactctt ttggattaaa gaaatagttg aacatgaatt
      961 taattatcaa cgccatattc acatggacag ttccctccag ctttggcaat gccctgatat
     1021 cgcgtgcagc catgtggaag tatgtatcct tttcaaaacc ttttgcagct atgatatccc
     1081 acagtaaatt gatgacctcc tcgccctgtg tggtcacttt catcttcacc attggattgt
     1141 acaaaaggta tcgtttgtca ttttcattgg cacagcgcat ataatcggtt gcacgcagag
     1201 caaaaagttt cattgcaaca agccgtgtat aggcatcaac aaaaaactgt ttaatatgcg
     1261 gaaagtcggt tacatatttg ccatacagat tacggtgtcc ggcatgattc aacgcttcat
     1321 aaaagcagtg tgttgcaata ccaattgaac cccatcctag attaaactta caaatattga
     1381 tagtgttcag cgatgaatcc catgcatcac gtcccttatc aaggatatca gcttcggcaa
     1441 ttggataatc gtgcagtgca tattcagcaa cgtatgattg attctgaata acatttttta
     1501 tacactcaaa tttttcatgc tgcgaatccg caacaaagaa aacataatca ccggtatcag
     1561 caaatttacc aaatgttgac accattgcag ctttattggc attccctata tagtatttat
     1621 caccattagc ccggtaagtg ccatcaccat taggatacag catcatatcg ctggaataga
     1681 tatcagcgcc atgttccttt tcagaaagcc caaaagcaaa gatagcgccc tctttcaaaa
     1741 gtttggcagc cttttgcttc acctcttcgt ttttgccaat ccatataggt cccagaccca
     1801 atgctgaaac ctgaaaggta taccagtagc aaagtccgta aaatcctaaa atttctgcat
     1861 agttaacaat cctgtaggta tcccatcggc tgtttttatc gccatatttt tctggagtaa
     1921 agaactttgc aaatatctgg ttatcctttt gaaattgtaa aaagtcatca taccacacat
     1981 agttgtgatc gtcatccaga atctttttta accccttggt ttcaaaaaaa tcaattgttt
     2041 tgcgcataat ctcttctgac tctttgtcag aaaactgcgc actgtacttc tttggattta
     2101 acagtatcat accccacctc tattcaaatt atagttacaa atttgaatat atcatgcacc
     2161 tgatgcattg tacagaaaaa attacaccgt tatgtatttt tttaaacaac ttatttcata
     2221 aattgtttac ctgtgttttg ggcgtgcccc tgccaacagt caaaatcatc ataccaacgg
     2281 aacaccacct ctattccctc tatgcataat cccgctgata ttatggcagg gtcgggctac
     2341 tgcaggctac gctatcgctt cggccacggg catgtatagt ataaaagctt tcacattatc
     2401 tatcaatacc aatccgtaat gcctatgtgt ctctacaaat aaacgcccgt ggctgctttt
     2461 caagcacctt gcgtatccct cacgcaaact atcgtgctaa tggaattacc ctgtctttta
     2521 tcctgccgct ttccaccagt tccataaact cttcagcaat ctgctgtcct aaagcaaccg
     2581 tttcattcca gaataacaat cgcctgctat cgtcacctgc aaaggtatag aaatccttgc
     2641 ggtcaggtat ctttttatac ctcagcgacg caatagcttc ttttgacggt gaaaccatac
     2701 acacattttc caatgcatgg tgcggctttc tatactttag tggtttatca aaccatcccg
     2761 gaataatcct gtcactgtaa tgaggataca gggcaatata gtcacccggt tcaagcggca
     2821 ggtcctgctg ataatcaaca agaccgccat cccgtagtat atgcgttttt ccctcatgct
     2881 gaaacacaac gccgttcata acaagaggga tggagccgga tgccaataca gcttttttaa
     2941 ggttgttgtt gttcaacggt atgtactgcg tatgatagtt tttccactgt ataaatgatg
     3001 gaaattcacg agcatcataa aaggcaaacc tatcaaaatg caatccaacc agctttctgc
     3061 tcaccatatt ggttattgca ttaattccca atccagcacc cagacggaat gtatcctcat
     3121 actgcaaaag atgccgtgag cgcgcagtga taatacaaag acgttgtgtg ggatgcttta
     3181 caatctcatc caccttgcta tcgggcaaaa attcttcaag aattgctacc gtttcggcag
     3241 ttatctcctc acggtcaggt ttcattgaat atttttgtgc aaagtagcgt tctttaaatg
     3301 cttcaatccc ctgcaatcca tcagcaagcg acaatgcagc aaaacgccat gttgcaatgg
     3361 atgagccaat catgtacaca ggatttttcc ttttttgtaa aaatgattta tacagtgctt
     3421 tatcaagccc gtacagtata atccatttgg gaccacctgc agcaccaagc atacacctaa
     3481 ttctttcttt ttgtattcct tcatctttaa taatggcgta tgcctttgca cctgcctgaa
     3541 ttgtacatat attcttcata acaacctctg tgataaaata attataaata acactgttat
     3601 catattttta cctgcaggca agaaaaaaat ttatagtaca catccatggt catcggaagt
     3661 ttctgatagt tttctcatta gattgtatca ttatattgtt ttttttgttg aaaacaatta
     3721 tcgaatctac gatagtctat agtcatgata gtatacgttt attaaacaac caacataaga
     3781 atgagtatat tttcctcata cataataact taattatcaa aatactccag gaccacggaa
     3841 aaaaagatat atgaaaaaat ccatagtttt aaccattatt gtattattat gcataagttg
     3901 ttcaaatccc catggtacat acgaattaca aacatcatct ccccaactca tctcacaact
     3961 tgaaagattt tcttatgata agtataatct tattgataat aaaatgtata catcatggca
     4021 ggtacgtaat aaaaataatt ggcatttaca aattcctcac ggggcggaag cctaagatgc
     4081 gaaacgtatc atttggatag ccatagttac aaacactgga ctccataagg tctgcatcat
     4141 agttccagct gccaccgcga ataatccgga ataccccata atcatcaatg tcccagtcaa
     4201 aacaccactc ccatacattc ccgctcatat catataatcc ccatgcattt ggcaatttag
     4261 tgcctacagg atgcgtctgt tcgtctgaat tagcattata ccagcaataa tcaccattta
     4321 cattgtcacc ccagtaatac cttgttgttg tgcccgcacg acaggcatat tcccactctg
     4381 cactctccgg caaccgaaag ccgttccctc cattaattgt tactgtccat tttattgtat
     4441 caccagtatt cttattctca gggtctttgt gaaccttatc aatagtataa tacggtttac
     4501 atcctgcttt aatgcttagc ttattgcaaa actcaacagc attgtaccag tttacccgtt
     4561 caaccggatt atcaggattc cctttgaaat aacttggatt tattcccata attgattcat
     4621 attgctgctg cgtaacctca cacatagcca tccagaatcc attcactttt actttattac
     4681 caccctgcat gaaacttccc gcaggtatct tcacaaaggc aataccctct cttacctcaa
     4741 ccagaccttt tttcttcaaa tttgatgata ggtatatctg cttaacggct ttttcctgac
     4801 ttacagcagt atcttcataa acaaaaagaa tggctatata tgttatcatc accacaacca
     4861 taagtatgtg ccatctatta atgattatag gtaaaatcat tgcatcctcc tttgctcatg
     4921 actgtttctg attaataaaa ttgactattc tttgatagaa caatagaaat gctctaagct
     4981 ggatttctaa aaagtcaagc taaaacgtac aagaatttta agaaataatt aaggaaaaca
     5041 agttgtacca taataacaat ccaattactt tacaaatatt ttttttaata gaaaacgctc
     5101 tatttatagt cattaataga gcgtttacag cataatacca attattgtaa aactatcaat
     5161 caataattgg tatgaaccct gtttctttta ctatagactg cccttcaggg gaaagaataa
     5221 aatcaataaa gttttgagct gcaggtttaa tttttacatc atttacatac atatacagtt
     5281 tacgagcgat ggggtactta cctgatttcc catttgggat ggttggctca acgctattta
     5341 caaacaaaac ttttactgta tcattaagat atccataccc aacatagcca atggcttttt
     5401 tattccttgc aacaaccgat aaaactgctc catttgatgc ttgaagcagc gcatccttgc
     5461 gcacctcaac atttttcatt acctttgatt cccatacttc ataggttccc gagcttgaat
     5521 cccgtgaaat aacaacaata gtttcatcat taccaccgac atcattccaa ttttttactg
     5581 aaccttcata gatagcctta agctgatgta aagtaagatt tccaactgga ttagaaggat
     5641 gcacaatggg aacaatcata tcataggcta ttgccactcc cttgattgtt tcaccttttc
     5701 gtttagccat ctccaattct acttcatgca tttcacgtga tgaattagct atatcacaac
     5761 tcccatcaat caacgccttt ataccatcac ctgaacccgt acctgacagt gaaatgacaa
     5821 ccttattctt ctcataaaaa acttcggcag atctctgagc tatagggagc actgtcgttg
     5881 aacctttaac aaccactttc tctttagctt cttttttgca cgccagcata agtaatccaa
     5941 ccgaaacaat caacagtacg gatatcacat aatttttaat tgccattttt taacctccat
     6001 aaaaactaaa attatcaacc tcacctcagc ctctcttctt agcaagatga ggtgagcgat
     6061 gttcccaatt tgcatcattt tcatacccta tgcgtcaatg catcacagac atcttctaac
     6121 cattctgcca aattctattg acttagttgt ccaaacacta ttttaactac acataataga
     6181 ttactataat aacaattcaa ttactttgca aatatttttt aaacaaaaaa cgctatatca
     6241 atatgcatca atatagcgtt tacagcataa taccaattat tgtaaagcta tcaatcaata
     6301 attggaataa acccagcttc tttcactatt atttgccctt catttgaaag gataaaatta
     6361 ataaactgct ttgcctcagg tttaattttt acatcattta catacatgta cagtttacga
     6421 gcgatggggt acttacctga tttgccattt gggatggttg gctcaacatt gtttacataa
     6481 atacccttca cggtcttatc aagatatcca tagccaacat agccaatggc ttttttattc
     6541 cttgcgacaa ccgataaaac cgctccattt gacgcttgaa gtaacgcatc cttccgcact
     6601 tcagcttttt tcattacctt tgattcccat acttcaaatg ttcccgagct tgaatcacgg
     6661 gacactacca caatcttttc attgtttccg ccaacctctt tccagttggt gatagttcct
     6721 tcatagatag cctttagctg ttccagtgaa agattcttta ccggattgga tggatgcaca
     6781 atgggaacaa tcatatcata agcgattgca acctctttta tcttttcacc tttttcttta
     6841 gccatagcaa gttcttccgg tttcatctct cttgatgagt tggctatatc acagctccca
     6901 tcaatcaacg actttatacc atcacctgaa cccgtacctg aaagactgat aataatcttt
     6961 gttttgtcat aaaacacctc aactgccttc tgtgcaattg gtaatactgt tgttgaacct
     7021 ttgataacaa ctttagcatc attgccttta gaaaatccca ttgatactga gaagacaaat
     7081 gctgttatca ttaacaccaa aaaactcttg accttcataa aagcctccct gtaaactatt
     7141 aatgagaaga ttttgcaatc tccattggtt gtataaatag tcatgcattg ttaaattttt
     7201 atgaagaatc aattaatttt atgttttttt atgtattatc gacaaatcgt aaaaaaccac
     7261 cttttcatgt gattaatcat tgaaaaaaat ggttggcaac tattttctga tacaattgta
     7321 tgaataatta ctattttaga aaccctatta tgctaatgaa aatggataaa catcttccaa
     7381 tcacaatcca gaagggctac tatagaagaa taacgtcttt atttttaaaa aaaatatatg
     7441 cttttataca tgactttatt ataaattgtc agtatttatt ttattactta aaaataattt
     7501 taaattgaaa tgaggtacac cccgccgtct tcggcgccgg ggtgtacatt tattattata
     7561 tgtacataat tttccgtttt ggaattaaca gttataaatc tttacatttt ctttacaatt
     7621 tcataatata ttatttataa taagatgggt tattattaat attaagaata ataacggtga
     7681 tatatgtctg aaaaagaaat aaaagtccaa gcaaaaaatc tttcgtttta ctatggtagc
     7741 agtcaggcgc tttttgatat agacctcact gtattagcta attcggtaat ggcattgatt
     7801 ggcccttcag gctgtggtaa atcaacattt ttacgttgta ttaacagaat gaatgatata
     7861 ataccgtata cgcacattgc tggagacatg accattgacg gtgaatccat ttatactata
     7921 aaatatgatg tcactcagct caggcgccgt gttggcatgg tatttcaaaa atcaaatcca
     7981 ttcccaaaat ctatttttga taacatcgca tatggattaa agattaatgg cataaaagat
     8041 cgctatatca ttgaaaaaac agttgaagat tctttaaaac gaactgcatt gtgggatgag
     8101 gttaaagata ggcttaatga ttcggcaatg agcttatccg gcgggcagca gcagcggtta
     8161 tgtattgcac gcactatagc agtgcaacct gagataatac tgatggatga acctgcatca
     8221 gcgcttgacc caatttcaac acaaaagatt gaagagctta ttcaggattt aaaaaagcaa
     8281 tataccataa ttatcgtaac acataacatg cagcaggcag cccgggttag cgattacaca
     8341 gcattttttt atatgggcaa actcatagaa gttaataaaa ccgaagtgat gtttaccaaa
     8401 cccgataagc agatgaccga aaattacatc accggaaaat ttggttaatt attgcaccta
     8461 aaggagataa caatgtctac tcatttagag caggaactta atgagctaat acggaagata
     8521 ctggagatgg cagatattgc catcgaagca gttgaagatt cagtccaatc actaaaaaat
     8581 attgatgtca aattagctga aaaagttatc cagaaagata ctgcccttga tacattagag
     8641 gtttttattg atgaagagtg tgtaagaatc cttgtaacaa agcagcccgc agcagcagac
     8701 ttacggctta ttttagcact atttaaatca aacactgatt tagaaagaat aggtgatttt
     8761 gcaaccaaca tcgcaaaaga gacaatacgg ttagagggca aatcactgct taaaccgctt
     8821 attgacatcc ctcgtatgga aaaaatttgc atcgatatga taaaactggc gtttagagca
     8881 atcaccgaaa agaatattga ttatgcaaag cgatgtatag atatggataa agaggttgac
     8941 gagcttaata tgcagatcaa ccgtgagctg tttagcttta tggtagaaaa tcctgcaacc
     9001 atatcgccag catttgggct tatcacggtt gcacgagcat tagagcgaat tggcgaccat
     9061 gcaaccaata ttgccgagcg tgcggtatac tatatacagg gcgaggacat acgacaccag
     9121 gagtaaacga tgtcaaaaat atatgttgtg gatgatgaaa aagatatccg tgaaattttg
     9181 aaggtgaatc tgcaaaaaaa cggctatgat gtcaatacct ttgcttctgc tgaagaagta
     9241 ctgaaacaac ttgctattgc aaaacctgat ttatttatat tggatatcat gatgaacggt
     9301 atggatggac tggatttatg caaacacatc cgtgcatcaa gagaattaac cactataccc
     9361 atcctatttc tttcagcaaa atctgccgaa ctggataagg tgctgggatt ggaactgggt
     9421 gccgatgact acctcaccaa acccttcagc attcatgagc tgattgccag agtaaaagca
     9481 atcatgcgcc gttcacaaac taaacaggaa ctatcgccaa aagaaatatt aacctataac
     9541 gcgatttcat tatatcctga taaatttcag gtaactatcg acaatcaaaa tattgacctt
     9601 acaaaaaccg aatttttaat cttaaaactt tttatgcagt atcccggtaa gatttttagt
     9661 cgcgacaata ttattgacag catccgtggt gataatgttt atgtcattga cagaaccata
     9721 gatgtccaca tcatgaattt gcgtaaaaaa ctgggaacat acaaagatgc tataaagacc
     9781 tattcgggcg ttggttatgg gctaaaagtt tgaaggagta aaactttttt ggtaaacagt
     9841 aatgaaaagg ctgaagacaa aacttgtagt agtatacacc gtcgccctgg taatctttat
     9901 ttcgtttctt ttgatgtata tcaatcacca cattcactct gctttcatcg catttcaaga
     9961 agcggagttg cagcaaaata ttgaaaacat acatatctat ttacattcct atcataaacc
    10021 tttcaattat gttctttttc aaaatcatga tgttgttgct tcattaaaaa atgtatcata
    10081 taaaagttcg ttgagaatta ctatcattac ggatgatgga actgtttatt ttgattcttt
    10141 tgccaatccg gagttgatgg aaaatcacct ttaccgcccg gaaatactgc aatcccgtac
    10201 aaaaggggta ggaagtgcaa tccgctatag caaaactgtc aaatctgata tgctgtatgt
    10261 tgcaaaatat tttgataact actatatacg aacggcaaaa tcacttggtg caatcaatat
    10321 acttattgga gccttaacaa aaaatatact catcgtggga tttgctgtgc ttgtgctgac
    10381 cattgctgca aattatattt ttgctacaat ctttacaaag ccaatagctg aatcattgca
    10441 atttattgat caattttttc atggcgatct aaacacccgc atcctcaact acaaagacga
    10501 tgagatgggc tatctacaaa attccatgaa cagattagcc gacaatatac aggcacgcat
    10561 aaacgagctc actgcccaaa agaataagct ctttatgata attgaaagca ttcatgatcc
    10621 aatagcgctc attgatgata gtaaaaaaat tactgtatac aataaagctt ttatagaact
    10681 gttccagttc cccgaaactg tcaccaatac ctattatttt tccattatac gccatagcca
    10741 gctcaatgca aagattcatg ttgcactatc acaacggcaa aatttttctt ttgaagaaac
    10801 cattggcagc aatcacttcc agatatttat ctaccctttt ccccagcctg atagtggcat
    10861 cttacttttg atgcatgatg taaccgagca aaaacgcatc cagaagttaa aatctgatct
    10921 tgttggcaac ctgtcacatg agcttaaaac acctatcagc attatccggg gttaccttga
    10981 aacaataaag gacatctccc tggacgaaaa aactgccggc caatttattg acaatgcact
    11041 tgccaatctt gaacggcaaa atgctatcat caatgatatg ctgaagctca atgaattgga
    11101 aacaactgcg tatgcagtta ctgaatatat tgatatcaaa actattatta cccggtgtat
    11161 tgaattgcta actccaaaaa tacaaaagaa aaatctggcg atagttatgg atattgatgc
    11221 aatccctgaa actattgccg gcaatgcatt cttaactgaa gagatttttt ttaacattat
    11281 tgataatgcc atcaattaca ataatcctaa tggagcaatc accattaaaa gcgctgttaa
    11341 aacagcaact atttcaataa gcatcgctga cacaggtgtt ggaatacctc aggatgaaat
    11401 accccgtatc tttgagcgat tttaccgcgt tgataaaagc cgttcacgtg atactggcgg
    11461 tacagggctt gggctttcaa ttgttaaaca tgcagctcag attttaggat ggaatgttag
    11521 cgttacctca tcaatgaatg gtacggtgtt tgccataaca ataccatggg cagggtaatt
    11581 ctttcatgaa ttagtttata ccctgctttg tttcaatagt atgttgagta tgttgagtat
    11641 gttgccctta ctaaatttac tttactattt catttataaa atcatctttt tttaaacttt
    11701 tctgccacat tctatgccgt ttaacagatt cgatcctttt atcacgagat atattcaaga
    11761 agcgcactgt ttcaaccgga cctaatgcat ctaacagaac atcgatccct ttctgtaaca
    11821 tctctttttc acttaaatac cttgatgctc tcattttaat ttctccttct cgcaaaatat
    11881 aacaggatta tatgatggga taatattaat atatttttta catttattca ataatttgtc
    11941 atcacatgta ataaatatag cattataata ttcagcaaat gcaatatgtg cggcatcggc
    12001 tataccaaaa cgcaatgata ttaatgtttc aattctgttt tcaatatatc agctttgaaa
    12061 ttatagattt taccatattt atttaataat tgcataattc agttctttcc agatcatcat
    12121 caattgattc aatttctttt ataagcacag gggaattaat taattcaaga tgtgaatctt
    12181 ttactttaga taatataaga ttaactgcat ttgtttctaa acgtatgcgc aaatagtgct
    12241 ggccatcaaa tggcctacat aatgagcaaa catccatata aattttattt gcatttttca
    12301 tcacaaacat aatatactaa gtaaaaatta tgtcaacaat gaattagctt cattcactgt
    12361 tttggaatac aggtatatgc ttacaataaa aaatatttca tttatccttt caatttcttt
    12421 ctatagctat agcgcatgat cattccggca agattaagtc caagtacaag gactatcagc
    12481 acgatagctg ttccatattg gatagggcgg gtagcttcta tgtgggtgcc ggcagttgcc
    12541 agaacataga tatggtatgg caatgccatg acctcactaa aaagcgaatc cggtacaaac
    12601 ggtgtgtaaa acgtggcaca ggtaaacatg atgggagctg tttctcccgc tgcacgccca
    12661 agtgaaagca tgatgccggt caatatgccg ggaagtgcag ttggcacaac tatcttataa
    12721 atggtttgcc acctggtggc acccagcgat aatgatgctt ccctatatga catgggaaca
    12781 gtaagtaaag cttcttctgt ggagcgtatg acaagcggaa gattaagtat tcccagcgtt
    12841 agcgagccgg caataatgga tgaaccaaaa tcaagaaata taacaaatac actcattcca
    12901 aaaagaccaa agactactga cggaacgcct gctaaattat taatacccag ccgtataaag
    12961 ttgagtactg ttacattttc aacatactcg gttaaataga ttgctgtcat aataccaatg
    13021 ggaacggata caagtgatga gcctatcatc aaatacaatg taccgataat ggcaggaaat
    13081 attccgcctt cacgcatctc attttttggg aagcttacta tgaaatccca tgaaagcgct
    13141 ttgtaaccct gcaaaaaaat atatcccaat acaaacagta aaaaaccaat gattgcatat
    13201 gacagtattg taatgataac aaatgaagag gtctcataat attttgcttt ttgttgtaac
    13261 gttgccatta tattaattcc atttaaactt atatttcatg gaaagatagt tggcaatgac
    13321 attaaacagc aatgtcagta caaagagtat gataccaatg gcaaacagcg caaaataatg
    13381 atcgctgcga tatgacgcct ctgccatttc ggcagctatg tttgctgtta aggggcgcac
    13441 cggatcaaaa agggatgtgg gaaagatagt agctccaccg gcaaccatca gtaccaccat
    13501 tgtttcaccc aatacacgtg aaatacccag aatgatagcg gtccataccc ccgaaagcgc
    13561 tgcaggcatg gtgatatgaa ctatcgtttg ccatctgtta gcacctaatg catacgatgc
    13621 ctcttttagt gatgctggca cggacgataa cgcatcctca gaaatactgg cgatagttgg
    13681 tatggccata aaggcaagca taatagcagc atttaataca ttaagtcctg tatcaatatc
    13741 aaaatgatcc tgcaaaaacg gggcaacaac caccatgcca aaaaaaccaa taatgaccga
    13801 tgggatggat gcaatgagct ctattatggg tttgactatt tcacggagag tggctggagc
    13861 taattccgaa agatatatag ctattgccat gctaaatggc acagcaaaag ctgctgcaaa
    13921 aaatgttacc gtcaacgatg caacaatcaa tggcaatgtg ccaaactttg gtggcacaga
    13981 tgttgggtac cagcttgtac caaacaaaaa ctccttcaca ctcacactat tgaatactgg
    14041 caatccctct ttaaaaagaa atatcataat taaaaacaaa aaagcaacag aaacaaaccc
    14101 tacaacagca aagatatacc gtataatcct gtcgctggca gtgtggatgt ttaaagctat
    14161 ttttaatctt tctttgagca tgatattata gtgtaaatgg tataatcaaa atcaagataa
    14221 tcaactttaa attagtcacc actatgtaaa gaaattataa atattttatg aagataacgt
    14281 taaaaatatt ctaaaatgat atcagatata gtgtcatttc tcataattta cagccttaca
    14341 tagatcatcc tgatatatag tacattcttt aatgattcta tcttttgcta atgcacaata
    14401 tgaagtatct aaatcaatcc ctattgcatt tctttttaag ttataggcag caataagcgt
    14461 tgttcctgac cccataaatg ggtctaaaat tatatcatcg ataaatgaaa ataattttat
    14521 gcaccgtttt ggtaattcaa ttggaaatgg tgcgggatgg cctatacgtt tcttgctttc
    14581 accggaaaat gtccataacc catttgtcca ttccataaat tcttcttttg ttatatccga
    14641 ctttctagaa cctgatgtct ttttccattt tttttatata atataacaat gagctcaaca
    14701 ggtgcaatca cgtactgcgc agaagcagat aaccatgaac cccatgcggt tcgtcgtgaa
    14761 atattgcctt cattc
//
LOCUS       DAOOJH010000059        14596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_3952_length_14596_cov_9.464480, whole genome shotgun sequence.
ACCESSION   DAOOJH010000059 DAOOJH010000000
VERSION     DAOOJH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3952_length_14596_cov_9.464480"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..549
                     /locus_tag="PLJ75_08435"
     CDS             172..549
                     /locus_tag="PLJ75_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05278.1"
                     /translation="MLFNNSLIIKSTITAAKSILCRDDFVHEDFFMALQQYLALTPFY
                     VRWIYSFGMYYCNLLSLIKQHTLFYKLPLDKSIQLLHSMEKGNLFINRSLIMMLKLFT
                     TMVYFDDDVHAQLIGYEHLAHCQ"
     gene            559..2067
                     /locus_tag="PLJ75_08440"
     CDS             559..2067
                     /locus_tag="PLJ75_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HPD05279.1"
                     /translation="MIYSYSDYEHSKQLSADVCVIGSGAGGAVAAYELAASGYRVIIV
                     EEGGFYSTKDYDGKPLTSMKAMWRGAGATVSFGIPSISIPAGKCVGGTTAINSATCFR
                     TPEHIVHQWKQRLQVNLDYKELETYCERVETIINVTKLPWDVLGNCANIVKRGADALG
                     LHCKPLMHNVKNCKGCGTCQFGCVEGAKQSMEVSYIPLALQYNAMLLTQCKAVKILFK
                     GNKAYGVQCAITNEAGRKATCTINASVIVCACGSMLTPLLLKQSGVRNKHLGKHLQIH
                     PCSRVVALMDEEVKGWIGVSQGAYIDDYADEGIMLEGIFVHPSILAASLPGVGNDFLQ
                     LVQRFNNIAAFGVMVHDTAEGVVSGNLNTPLLWYSMSGKDIETLKKGIAYLSMVFIAA
                     GAKQVFTPLSPFPVLHSADDVKKLEHAHITRKDIPEVFAFHPLSTCRMAGDSSCGVVD
                     SYGKCFNTDNVYIADGSIVPTSLGANPQITIMTLATKISRHIADTLAHTRCV"
     gene            2067..2726
                     /locus_tag="PLJ75_08445"
     CDS             2067..2726
                     /locus_tag="PLJ75_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05280.1"
                     /translation="MKMKKVMVTVCLLLFMSMNIFYDAYPDTLPGLTHDDIQKLQAGT
                     IIITQQKQNTADSQIARVTGAICIHKPINEVWKCLVDWETMPRYVDSLDYYKVIAKMT
                     DDVWIIEGQLHVTFIKFRYTLIVNSNKSKYYQQWRLLQHNDIAQYNLQNKTNPNSGGI
                     KNIEGYQYCIPYDDTTTILYYAPVVAVSVPVPGFIEDSLTKKSIRDYLYGIKNYLEKT
                     Q"
     gene            2918..3385
                     /locus_tag="PLJ75_08450"
     CDS             2918..3385
                     /locus_tag="PLJ75_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05281.1"
                     /translation="MNKKIIRLITVTGLLYTIAFTVTAFVFIFLHPQLMAIINAVSQK
                     IFPALPLAHEQSQFYKILCLSMMSGVTVCSLLLYKDAQTYIAMVIPLCTMKFTSSLCG
                     LTAFIGGSVFHNGWNNLANIIIVITDFPLGLWIFCLFIVYRRYNNECKNYDSQ"
     gene            3434..4447
                     /locus_tag="PLJ75_08455"
     CDS             3434..4447
                     /locus_tag="PLJ75_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05282.1"
                     /translation="MNKEELMISANYVQYNPDNRGHYESFFIRANHPQKPLAFWIRYT
                     IFQPAKNHRNALGELWAIVFDGEGHHRALKAEYPISQCSFAPDDFNIVIGDAVLMPGI
                     AYGCIAYGDETIQWDVTYSGNSDPLFLYPAKLYTAKLPKAKTLVGLPFARFNGSIQIN
                     GETIEIMNWVGSQNHNWGSKHTDHYAWGQVCGFDNEPDAFLELATARIKVGPLFTPFM
                     TPLVLRYKGNEYALNSLSKLLCRARFGYFYWDFKAVEKNSTVKGSIVALKEDFVCLPY
                     YNPPGGIKYCLNTKIASCEITCKVGNDQWTLKTKNRCAFEILTDDTMHGLQPIREIAV
                     IKN"
     gene            complement(4450..5709)
                     /locus_tag="PLJ75_08460"
     CDS             complement(4450..5709)
                     /locus_tag="PLJ75_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HPD05283.1"
                     /translation="MNHYKQMYITIRCITLPIILCLSIIALPSCSDSTNGYVPSWTIM
                     VYMDGDNNLERYALADFNEMEQVDTNGHSINVIVLLDRCDGETTADGDWKGTRLYKIL
                     HDTNSSKINSERLADSRYLKITNIGDSDELNMGNPDTLSNFISFGKENYPADYYSLIL
                     WDHGSGWRSTNSTALIASEPQTFKAVCVDATSANDILEMKEVKQALLGKGLTLIGFDA
                     CLMSMAEVAYQLKDCARYMVASEDLEPASGWLYSNFLNYFIASGRSPIDLGQSIIDAY
                     ALYGSYPMSMVDLSNIDVMTGAIDSLSSALITHIDAIKDTISTARDKTLAFQSSSSPP
                     YAYIDLYDFLLNISSVNEVSYEVNDAMNAVKSAVLYHYRGDYTHSNGLSIYFPQSDTD
                     SEYSSYNTYTIDFAQTLWDDFLAEYFQ"
     gene            6003..7640
                     /locus_tag="PLJ75_08465"
     CDS             6003..7640
                     /locus_tag="PLJ75_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05284.1"
                     /translation="MINIKRLLIKHFNIIYIFIPFIIITVLSGIISLYTTHEYNSYIS
                     QFIESLETAKNIQIELQKQFNNGKIIILEGISFNTYQHKIHEYSYNAQNIQNQLFNLG
                     IMCKDMKGIHVKIIKFTNFYKQVNNEFLSYIVNYNDLDFYSSKNIINNSFANEQKLLQ
                     MMEDIVSDIDKVSNEKIQYINKYYLTGGFISIGLLTIFALVVSWFSARLFNRFKEKLE
                     TKVQRRTEQLQKTNDKLSLSEQKYRFLIESTDDMIFTMSEQGEIFTINKSVNEYLKLK
                     SSAVIGNNFYDYLYFDNDNDVFKKQMLKKNIERSLYDKKKMGFYAEFKTNKMIEPIEM
                     FVTLESVTTNGRMEIIGKASKIIENEIIDSFLYEHVKYEISNSLLLADEITHRIILNV
                     KKFLPHNAIAQLRLVISELLINAIEHGNLDITYEDKLLKLQDDVYFDYMTQKINEKDN
                     KDKKIKVEFMVNSERIVIKISDQGKGFDHKKYLSRSAEQITEESLLQHGRGILLARQI
                     FDEIKYNDKGNQVLCIKYFNPVEKELHAHKKDQILLS"
     gene            7958..8599
                     /locus_tag="PLJ75_08470"
     CDS             7958..8599
                     /locus_tag="PLJ75_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05285.1"
                     /translation="MKKYTITLYTIIFLLICSASYSQQLSPIVKLYPFNAADTYLINW
                     KWITALDYKKISEWAKIYRCSVNYDDYPIIDGVAWFVSKYGSTIIFDNLDDIKYFLHI
                     NFVTFDDPEKTGIDALCIVSCNGKPVKRLAFSDMIVNNSRVTIEITREYIVNKTMLVE
                     FKEYSSAGGFFGVWDVVLSKSDVLPQIIDIPESKSSKSKKGTMETIPQTLIKP"
     gene            8904..9191
                     /locus_tag="PLJ75_08475"
     CDS             8904..9191
                     /locus_tag="PLJ75_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05286.1"
                     /translation="MANEFEKEFDDEVYEDDFYEESFDDEELLEEESIEDDAQDEQEE
                     IEEDEKHIAVILACDDCDYRWEDVIVKGKDEDIEDLEIICPMCGSTNVTQI"
     gene            9188..12013
                     /gene="uvrA"
                     /locus_tag="PLJ75_08480"
     CDS             9188..12013
                     /gene="uvrA"
                     /locus_tag="PLJ75_08480"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPD05287.1"
                     /translation="MSQQFIRIKGAREHNLKNISVDIPRERLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMEKPDVDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIHDYLRLLFARVGIAYCYQCGKPISSQTIDQIVENVLTHPQGTKVQVLAPVVRGR
                     KGMYQDLFEKMKQSGYSRVRVDGQLHLLDDPIAIDKNKKHSIDVIIDRITLKENIRAR
                     LSDSIETAIQLAGGQVVLIINDNEQLISTKMTCPDCDISLPELSPRMFSFNSPYGACP
                     SCDGLGFIMQFDPDLIVDNPDKSLMDGALAVWGKTTNYWYQEQVVALARHLKFDPYTP
                     WKKLSRSIQQVVLYGSNGKKIDYSIERHEAQYTFAKAFEGVISNLTRRYKETQSDEMR
                     EWMERFMRNRVCDACGGRRLRPESLAVKVADKNIADISALSIKDAVTFFHELSLSETQ
                     MAIARQIVKEITARLLFLSNVGIDYLTLDRAAGTLSGGEAQRIRLATQIGSGLTGVLY
                     ILDEPSIGLHQRDNKKLLDTLKELRDIGNTVIVVEHDEETIRESDYVIDLGPGAGLDG
                     GYIIAQGTPEEIENNESSITGLYLNGKRFIAQPQTRRKSEHHLVIRGASENNLKAIDV
                     SFPLGVFTCITGVSGSGKSTLIIDILYKALASLKMHSKDYPGKFKKIEGIEYIDKVID
                     VDQSPIGRTPRSNPATYTGTFTPIRELFANLPESKIRGYKPGRFSFNVSGGRCEACWG
                     DGIKKIEMHFLPDVYITCEVCKGKRYNHETLEVRYKGKNIYEVLEMTVDEAYDFFYSI
                     PSIKQKLETLKRVGLGYIKLGQPATTLSGGEAQRVKLSTELSKRATGNTLYILDEPTT
                     GLHFADVAKLIDVLQSLVDKGNTVIVIEHNLDVIKCADWIIDLGPEGGDAGGRIVAEG
                     SPEDIAKIKQSYTGQFLKKIVLHKKIN"
     gene            12101..>12462
                     /locus_tag="PLJ75_08485"
     CDS             12101..>12462
                     /locus_tag="PLJ75_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05288.1"
                     /translation="MKRITTTSFFVIFIVLSSAVFPLDNNVVIFQKAKEDVMKGMYHF
                     NEMHYLAAIEFFRKALEVYPDYYTASEYLARSYKLAGYTDLALQQWQLLLEMTKGNVL
                     VQNKIDMINFRSSLYNGL"
     assembly_gap    12463..12562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12563..13319
                     /locus_tag="PLJ75_08490"
     CDS             <12563..13319
                     /locus_tag="PLJ75_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD05289.1"
                     /translation="RDRYGRPIYGLTQDNYTVVEDGATISTLNVDYLKNKMPAASMVL
                     CVDRSQPLQRYHNDIPWLAEFILKKMHKNDKLKILNFAADTWVANPYDWSRRRALKAL
                     KNFDYDSGRDIGKALYMAISDVLPQMERRGVILITDGDTAEKDFKAYSADIIIDYAKS
                     HFIPVYILTLKTKSPLLMRIANETGGAIYKGSELDGLRTVYDAIKNSNDYRYVLLYHT
                     FKMKSLTGWWSDVTINVSYKGQKGTEWAGYFVP"
     gene            13751..14416
                     /locus_tag="PLJ75_08495"
     CDS             13751..14416
                     /locus_tag="PLJ75_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05290.1"
                     /translation="MNNQTKSFLTTCVFLFMIIMLNGCVTSSYFTLTENQKPPIVPDT
                     IAVISGSASEIDIHLANKLTEELSNTKFKVMSQEQITKIIPDYPLNIINFTCEGKKFK
                     QPYISDESKKIVDAMQSKLKVKYVLLIWNNGVGTVTYTNRYGQQSGKEVEVCILTRFL
                     KYPEATIVGYSFFCDSKSLGYFFMINEDKKIAKTVEELLNKGVKKIVKEIVKNTENPA
                     GKK"
BASE COUNT         4806 a         2336 c         2937 g         4417 t
ORIGIN      
        1 cagtatgagg aggcgtaccg taagcgctac tccattgcac agtgggtata aaaaaggggt
       61 cagagcttta taataattaa aaaaagtacc aaaataaaaa tatatgttga cataatatat
      121 agccccaata atatgtactt aaagtctata ttattgggag gaatacttac catgcttttt
      181 aataactcgc tgataataaa aagtactata acagcagcaa aaagcatact atgcagagat
      241 gattttgtcc atgaggattt cttcatggca ctacaacaat atttagctct gacccctttt
      301 tatgtacggt ggatatatag tttcggcatg tattattgca atctgctatc acttataaaa
      361 caacatacac ttttttataa actgccactg gataaatcaa tacagcttct gcatagcatg
      421 gaaaaaggca atttgtttat caacaggtca ttgataatga tgctaaaatt attcaccacg
      481 atggtatatt ttgatgatga tgtgcatgca caattgattg gctatgagca tctggcacat
      541 tgccaataga ggcatgatat gatatattca tacagcgatt atgaacacag caaacaattg
      601 agcgctgatg tttgcgtcat tggcagcggt gctggtgggg cagtggctgc ctatgagctt
      661 gctgcatcag ggtatcgtgt tatcattgtt gaagagggcg gtttttattc aacaaaagat
      721 tatgacggca agccacttac cagtatgaaa gcaatgtggc gtggtgcagg tgctactgtt
      781 tcgtttggca ttccgtccat atcaattcca gcaggcaaat gtgttggtgg cactacagcc
      841 attaattctg caacatgttt cagaacgcca gagcatattg ttcatcaatg gaaacaacgg
      901 ttacaggtaa atcttgatta taaagaactt gaaacatatt gtgagcgtgt tgaaacaata
      961 atcaatgtta cgaaactgcc atgggatgtg cttggcaatt gcgcaaacat tgttaagcgg
     1021 ggtgcagatg cgcttggatt gcactgcaaa ccgttaatgc acaatgtaaa aaattgtaag
     1081 ggttgcggta cctgtcagtt tggctgtgtt gaaggagcca aacaaagtat ggaggtaagc
     1141 tatattcctt tagccttaca atacaacgca atgctattaa cacagtgtaa agcggtaaaa
     1201 attcttttta aagggaataa agcgtatggt gttcaatgtg ccataaccaa tgaagcaggg
     1261 aggaaagcta cctgtactat taatgcttct gttattgttt gtgcatgcgg cagcatgctt
     1321 actccgctgt tgcttaaaca aagtggcgta cgcaacaaac atcttggtaa acatttgcag
     1381 attcatccct gctcccgtgt tgttgctctc atggatgaag aggttaaagg ctggattggc
     1441 gtttcgcagg gagcatatat tgacgattat gcagatgaag gtattatgct ggaaggcatt
     1501 tttgtacatc cctcaatatt ggctgcatca cttcccggtg tgggaaatga ttttttacag
     1561 ttggtgcagc ggtttaacaa tattgcagcg tttggggtaa tggtgcatga tactgccgag
     1621 ggcgttgtat cggggaattt gaatacgcca cttttatggt attccatgag tggcaaagat
     1681 attgaaactt taaaaaaagg catcgcatac ctgtccatgg tttttatcgc tgccggagca
     1741 aaacaggtct ttacgccgct atcgccattt ccggtgctcc attcagcgga tgatgttaaa
     1801 aaattggaac atgcccatat cacgcggaaa gatatcccgg aggtttttgc ctttcatcca
     1861 ttgagtacat gtcgtatggc gggcgatagt tcctgtggag tggtcgatag ttatggtaaa
     1921 tgttttaaca ctgataatgt atatattgcc gatggcagca tcgtgccaac ttcactggga
     1981 gcaaatccac agattaccat tatgacttta gctacaaaga tttcgcggca cattgctgat
     2041 acattagctc atacacggtg tgtgtgatga agatgaaaaa agtcatggtg acagtatgtt
     2101 tgctactatt tatgtcaatg aatatttttt atgatgccta tccggatacg ctgccaggtt
     2161 taacccatga tgatatacaa aagctgcagg ctggtactat cattataaca cagcaaaagc
     2221 aaaatactgc tgatagccag attgcccgtg taacaggggc tatttgtata cataaaccta
     2281 tcaatgaggt gtggaaatgc ctcgttgact gggaaacaat gccacgctat gttgatagcc
     2341 tggattatta taaagttatt gcaaaaatga ctgatgatgt gtggataatt gaaggacaac
     2401 tgcatgttac atttataaaa tttagatata cactcattgt caacagcaat aaaagtaagt
     2461 attatcaaca atggcgactg cttcagcaca atgatattgc acagtataat cttcaaaata
     2521 aaacaaatcc aaacagcggt ggtataaaaa atattgaagg gtatcagtac tgtattccct
     2581 atgatgacac tacaacaata ctgtattatg ctccggttgt tgcagtatca gtgccggtac
     2641 cgggttttat tgaagatagc ctcaccaaaa aaagtatcag ggattatctg tatggtatta
     2701 agaactatct tgaaaaaaca caataatata gcattcaaca atagtcggat tatggttagc
     2761 caaacatgat acaaaagaat aagaaccctg caggggatat tgtattgtac tatagtaagg
     2821 gcgtattgcg atacgcccac aaaaacaata catcattttc tggaaaagcc cgtaatgaca
     2881 ttgagggaat catcacatga ttggaggatt attttttatg aataaaaaaa tcatccgttt
     2941 gattaccgta acagggttgc tttacacaat agcgtttacg gttacagcat ttgtttttat
     3001 atttttacat ccacaactga tggcaattat taatgcagtg tcgcaaaaaa ttttcccggc
     3061 actgccactg gcacatgaac aaagtcagtt ttataaaatc ttatgtttat caatgatgtc
     3121 gggagttact gtctgttcac tgctcttgta taaagacgcg caaacatata ttgctatggt
     3181 tataccactg tgcactatga aatttacatc gtcattatgc ggattaacag catttatagg
     3241 gggaagtgtt tttcacaatg gctggaacaa tctggcaaat ataataattg ttataacaga
     3301 ttttccttta gggctatgga ttttttgctt gtttatagta tacaggcggt ataacaatga
     3361 atgcaaaaac tatgattcac agtaactatc gccaaaaata ttttagtaca tacctaacga
     3421 caggaggttc tttatgaata aggaagagct tatgatttca gccaactatg tacaatataa
     3481 tcccgataac agagggcatt atgaaagttt ttttatccgt gccaatcatc ctcaaaagcc
     3541 attagcgttc tggatacgct ataccatctt tcagcctgca aaaaatcata gaaatgcttt
     3601 aggggaattg tgggcgatag tttttgatgg agaaggtcat catagagcat taaaggctga
     3661 gtatccaatt tcacaatgca gttttgcacc ggacgatttc aatattgtga ttggagatgc
     3721 agttttaatg cctggaatag cctacgggtg cattgcgtat ggtgatgaaa caatacagtg
     3781 ggatgtaacc tattcaggga acagcgaccc gctttttttg tacccggcaa aactttacac
     3841 tgcaaaactt cccaaagcaa aaacgctggt gggtttgcca tttgcacggt ttaatggttc
     3901 tatacagata aatggtgaaa cgatagagat aatgaactgg gttggaagcc aaaatcataa
     3961 ctggggttca aagcataccg accactatgc gtgggggcag gtttgtggct ttgacaatga
     4021 gccagacgct tttctggaat tggcaacagc tcgcataaag gtagggcccc tctttactcc
     4081 gtttatgacg ccactggtgt tacgatacaa aggaaatgaa tatgcactca attcgttatc
     4141 aaaattgcta tgcagggcac ggtttggtta tttttattgg gactttaaag cagtggaaaa
     4201 aaatagtact gtgaaaggtt caattgttgc attgaaagaa gattttgtat gtcttcccta
     4261 ttacaatcca cctggtggta taaagtattg cctcaacacc aagattgcat catgtgaaat
     4321 aacctgtaaa gtgggcaacg atcaatggac cttaaaaaca aagaatagat gtgcctttga
     4381 aatactgact gatgatacca tgcatggttt acagccaatc agggagatag cagttattaa
     4441 aaattgattt tactgaaaat attctgccag gaaatcatcc cacagggttt gtgcaaagtc
     4501 aatagtatag gtattgtagc tgctgtattc gctatcggta tctgattgtg gaaaatagat
     4561 ggataatccg tttgaatgtg tgtaatcacc acggtaatga tagagcacag cacttttcac
     4621 ggcattcatt gcatcgttta cttcgtaaga aacttcatta acagatgata tgttcaacaa
     4681 aaaatcatac aaatcgatgt atgcatacgg tggtgatgat gaactttgaa atgctaacgt
     4741 tttgtcacgt gctgtgctaa tggtatcttt aatagcatca atatgtgtta ttaatgctga
     4801 tgataatgaa tcaatggcac cagtcataac gtcaatgtta ctgagatcaa ccattgacat
     4861 ggggtaggaa ccataaaggg cataagcgtc aataattgat tgtcctaaat cgatggggct
     4921 tctgcccgat gcaataaaat aattaaggaa gttgctatag agccagccac ttgcaggttc
     4981 taaatcttca gatgcaacca tgtagcgcgc acaatcttta agctggtagg caacctctgc
     5041 catactcata aggcatgcat caaaaccaat gagcgttagc ccttttccaa gcaatgcctg
     5101 cttcacctct ttcatttcca aaatatcgtt agcgcttgta gcatccacac atactgcttt
     5161 aaatgtctga ggttcagatg caatcaaagc agtgctattg gtgctgcgcc atcccgaacc
     5221 atggtcccat agtataaggc tataataatc tgccggataa ttttctttac caaaagaaat
     5281 aaaattactt aaagtatcgg gatttcccat gttcaattca tcgctatcgc cgatgtttgt
     5341 tatttttaaa tagcggctgt cggctaatct ttcagagttt atttttgagc tgttggtatc
     5401 atgcaaaatt ttatacagac gagtgccttt ccaatcacca tcggctgtcg tttcaccatc
     5461 acaacggtca aggaggacaa tgacattgat gctatgcccg tttgtatcca cctgttccat
     5521 ctcattaaaa tcagcaagtg cataccgttc aaggttgtta tcaccatcca tatataccat
     5581 gatagtccat gatggcacat agccgttagt gctgtcactg catgaaggta atgctatgat
     5641 gctaaggcaa agtatgattg gcagtgttat gcaacgtatg gtgatgtaca tttgtttata
     5701 atggttcatg gtatatccct gtttgtataa acatagtagg ttgtatatcc agtatatagg
     5761 caatcatttt tctgattcag gttataaatt actactatac aatgtaatgg tatccttgtt
     5821 gttatcaagc aacaactatc tactttgtat caatttatat taatggtttt attggcatgt
     5881 aatagtaaat tttacaatat aattatgcta tcttcataat tttttaaaca cgtgttgatt
     5941 cattaatctt taaattcaat actaaaattt atataaaaat attaatggca tggttgtggt
     6001 ttatgattaa tatcaagcgt ttactaatta aacattttaa tataatatat atttttattc
     6061 cttttattat aataactgtt ttatcaggta ttataagcct gtatactacc catgaatata
     6121 atagctatat atcacagttt atagaatcat tggaaacagc caagaatatt cagattgaat
     6181 tgcaaaaaca atttaataat gggaaaatta taattcttga aggtatttct tttaatacct
     6241 atcaacataa aattcatgaa tattcgtata acgcacaaaa tatccagaat caattattta
     6301 atctgggcat tatgtgcaag gatatgaaag gcattcatgt gaaaattata aaatttacaa
     6361 atttttataa gcaggtaaac aatgagtttt tatcctatat agtgaattat aatgatctgg
     6421 atttttatag cagtaaaaat attataaata attcatttgc aaatgaacaa aaattgttac
     6481 aaatgatgga agatatagta agcgatatag ataaggtttc aaacgaaaaa atacaatata
     6541 taaataaata ttacctgaca ggtggtttta tttccattgg tttgttaact atatttgcac
     6601 tggtagtgag ctggttttct gcacgtttgt tcaacaggtt taaagaaaaa cttgagacga
     6661 aagttcagag aagaactgaa cagttgcaaa aaacaaatga taaattatca ttatcagaac
     6721 aaaagtaccg gtttttaatt gaatcaactg atgatatgat ttttacgatg agtgagcagg
     6781 gggaaatatt taccattaat aaatctgtta acgagtatct aaaattaaag tccagcgctg
     6841 tcattggtaa taatttttat gattacctgt attttgataa tgacaacgat gtgttcaaaa
     6901 agcaaatgtt gaaaaagaat attgaacggt cattatatga taaaaagaag atgggatttt
     6961 acgctgaatt taaaactaac aaaatgatag aacctattga aatgtttgta acacttgaat
     7021 ccgttaccac taatggaagg atggaaataa ttggcaaagc ctcaaagata atagaaaatg
     7081 aaattattga ttcatttctt tatgaacatg taaaatatga aatttcaaat tcattacttt
     7141 tagcggatga gattactcat agaattatat taaatgttaa aaaatttttg ccgcacaatg
     7201 caattgctca gttgcgatta gtaatcagcg aactgcttat caatgcaatt gaacatggta
     7261 atttagatat aacctatgaa gataaattat taaagcttca ggatgatgta tattttgatt
     7321 atatgacgca aaagattaat gaaaaagata acaaagataa aaaaatcaag gtagaattta
     7381 tggttaatag tgaaaggata gtaattaaga tatctgacca gggaaaagga tttgatcata
     7441 aaaaatattt aagcaggtct gctgagcaga ttactgaaga atcattatta cagcatggta
     7501 gagggatttt acttgcaaga cagatatttg atgaaataaa atataatgat aaaggtaatc
     7561 aggtgctatg cataaaatac ttcaatccag tggaaaaaga actacatgca cataaaaaag
     7621 atcaaatact gttaagttga aataagcatt gcagttttac tctgcaatat ttatacggga
     7681 acattattgc atatacattc taatggataa tcaatttaca tgttcaatat attttttatt
     7741 tagtatctgt attgtattca attaaattgc agattcatca ttacaacaca gtgagaggtg
     7801 ccgataataa catgcttttg ctatacatag accccgtatt ttgattaaag tctctatatc
     7861 attgcacaaa aataatgata atcaaagtat tcctgaaaat attttgcatg taaaaaattt
     7921 cttgccttct atgtattatc atacaataat tgtaacaatg aaaaagtata ctataacatt
     7981 atatactata attttcctgc ttatatgttc tgcatcatat tcacagcagc tatcgcccat
     8041 agtaaaattg tacccattta atgctgctga tacttattta ataaactgga agtggataac
     8101 cgctcttgat tataaaaaaa tttcggagtg ggcaaaaatc tatcgctgca gtgtaaacta
     8161 cgatgattat cccatcatag acggggttgc atggtttgta tcaaaatatg ggtcaaccat
     8221 catttttgat aatcttgatg atataaaata ttttcttcat ataaattttg taacatttga
     8281 tgatccagaa aaaaccggga ttgatgcact gtgtattgta tcatgtaatg gtaaacctgt
     8341 gaaaagatta gccttcagtg atatgattgt caataacagc agggtaacca ttgaaataac
     8401 acgggaatat attgttaaca aaacaatgct tgttgagttt aaagaatatt cttctgctgg
     8461 cggttttttt ggtgtgtggg atgttgtttt aagcaaaagc gatgtgcttc ctcagattat
     8521 agatattcca gaatcaaaaa gttcaaaaag taaaaaagga actatggaaa caattccaca
     8581 aacactgata aagccttaaa gctaaaaaac aaaaaaaata gtgttttgta tattttgtag
     8641 taaattacaa tttttcagtg tagcaaacca attttttttt aattattact gaaattttat
     8701 tgaccatttc aacgatacta acttttgtga caaaaaggaa tatgaaaatt gctattgtga
     8761 aattttaacg acagtattgg ttttgtcacc tttgtgataa aaatttttta ttttttcatt
     8821 tttttgttag atagtttttt aagaattcat atattgttaa tagtatttta tagtaaaata
     8881 ctgcttaaaa agggtgatag cgtatggcaa atgaatttga aaaagagttt gatgatgaag
     8941 tatatgaaga cgacttctat gaagagagtt ttgatgatga ggaattactt gaagaagaaa
     9001 gtatagagga tgatgctcag gatgaacagg aagagattga agaagatgaa aaacatatag
     9061 cggtaatttt agcctgtgat gattgtgatt accgatggga agatgtgatt gtaaaaggta
     9121 aagacgagga tattgaggat ttagagatta tatgtcctat gtgcggttct actaatgtaa
     9181 cccagatatg agtcagcaat ttattcgtat taaaggtgcc cgtgagcaca atttaaaaaa
     9241 tatatcggtt gatattcctc gggaaaggct ggtggttatt accgggcttt ccggttcagg
     9301 aaagtcatct ctggcttttg atacaattta tgcagaaggt cagcgcaggt atgttgagtc
     9361 gctgtcagcc tatgctcggc agtttttagg acagatggaa aaaccggatg ttgacctcat
     9421 tgaaggacta tcgcctgcta tatcaataga acaaaaaaca actcatcgta atccccgttc
     9481 aaccgttggt actgttactg aaattcatga ttacctgcgt ttattatttg cccgtgttgg
     9541 tattgcgtac tgttaccaat gcggcaaacc catttcttcg caaaccattg accagattgt
     9601 tgaaaatgtg ttaacccatc cgcagggaac aaaggtgcag gtgctggcac cggtggtcag
     9661 gggcagaaaa gggatgtatc aggatttgtt tgaaaagatg aaacaatcgg gatactcacg
     9721 ggtcagggtg gatgggcagt tgcatttgct ggatgatcct attgccattg ataaaaacaa
     9781 gaagcattca atagatgtca tcattgatcg tatcacactg aaagaaaata ttagagcgcg
     9841 gttatcagat tccattgaaa ctgctatcca gttagccgga gggcaggttg tattaatcat
     9901 caatgataac gagcaactta tttcaacaaa aatgacctgt cctgactgtg atatttcatt
     9961 accggaacta tcgccaagaa tgttttcatt taatagtccg tatggcgcct gtccgtcatg
    10021 tgatgggctt ggctttatta tgcagtttga tcccgatttg attgtggata atcctgataa
    10081 atcgttgatg gatggtgcat tagcggtatg gggaaaaaca acaaactact ggtatcagga
    10141 acaggtagtg gcgcttgccc gtcatttaaa gtttgatcct tataccccat ggaaaaaact
    10201 ttccagaagt attcagcagg ttgtactcta tggatcaaat ggtaaaaaaa ttgactactc
    10261 cattgaacgt catgaagcgc agtatacatt tgcaaaagca tttgaagggg ttatttccaa
    10321 tttaacccgt cgctacaaag aaacgcagtc ggatgagatg cgcgagtgga tggaacgatt
    10381 tatgcgcaat cgtgtatgcg atgcatgcgg aggtagacgt ttgcgtccgg aaagtctggc
    10441 ggtaaaggtt gctgataaaa atattgctga tatcagtgcg ctgtcaatca aggatgcagt
    10501 aacatttttt catgagcttt cgctttcaga aacgcagatg gcaattgccc gtcagatagt
    10561 gaaggagatt acggcaaggc ttttattcct ttcaaatgtt ggtattgatt atctgacgct
    10621 tgaccgagct gccgggacac tttcaggcgg tgaggcacag cgtatacgcc ttgccacaca
    10681 aataggttcg gggttaaccg gggtgctgta tattctggat gaaccaagca ttggattgca
    10741 tcagcgtgat aacaaaaaac ttctggatac attaaaggaa ctgagggata taggcaatac
    10801 ggtgatagtt gttgaacatg acgaagaaac cattcgcgaa tcggattacg tcattgattt
    10861 aggacctggt gcagggcttg atggtggata tatcattgca caggggacac cggaagagat
    10921 tgaaaataat gaaagctcaa taacggggtt atatcttaac ggcaagcgat ttattgcaca
    10981 acctcaaacc cgtagaaaaa gcgagcacca cctagtgata cgtggcgctt ctgaaaataa
    11041 tctaaaagct attgatgttt catttccttt aggagtgttt acctgtatta ccggtgtatc
    11101 cggttcgggc aaatcaacct tgattattga cattttgtat aaagcactgg catcgttaaa
    11161 gatgcacagc aaagattatc ccggtaaatt taaaaaaatt gaagggattg aatatatcga
    11221 taaagtcatt gatgttgatc agtcgcccat tggcaggacg ccgcgctcca accctgctac
    11281 ctataccggt acatttactc ccatacggga gctgtttgcc aatttgcccg aatctaaaat
    11341 acgcggctat aagccgggac gtttttcgtt taacgtgagt ggcgggcgat gtgaggcgtg
    11401 ctggggtgac ggcataaaga agattgagat gcatttttta cctgatgtat atattacctg
    11461 tgaggtgtgc aaaggcaaac gctataatca tgaaacgctg gaggtgcgct ataaaggtaa
    11521 aaatatctat gaagtgttag agatgaccgt tgatgaagcg tatgattttt tttatagcat
    11581 accttcaatt aaacaaaaat tggaaacatt gaaaagggta gggctgggat atataaaatt
    11641 gggacagcct gccaccacac tttcgggcgg agaggcacaa cgtgttaaat tgtcaacgga
    11701 gctttcaaaa cgcgccacgg gcaataccct gtatatactg gatgaaccaa caacaggttt
    11761 gcattttgct gacgttgcga agcttattga tgtattgcaa agtttagttg ataaaggcaa
    11821 tacggtgata gttattgaac ataatctgga tgtcatcaaa tgtgccgact ggataattga
    11881 tctgggacct gaaggcggtg atgccggcgg caggattgtg gcagagggaa gccctgagga
    11941 tatagcaaag ataaagcaat cctatacagg acaattttta aagaagatag tattacacaa
    12001 aaagataaat taattgcaat aatttacact atacacttac cctgtacata aaaccgatac
    12061 tcataaaggg gtgcattatt atattccatg gagtaatact gtgaaacgaa taacaacaac
    12121 gagttttttt gttatattca ttgtactttc ttcggcagtt ttcccattag ataacaacgt
    12181 ggtgatattc cagaaagcca aagaagatgt tatgaaaggc atgtaccatt tcaatgaaat
    12241 gcactacctt gctgccattg agtttttccg aaaagcgtta gaggtttatc ccgactatta
    12301 taccgccagc gaatatctgg cgcgttcata taaattggca ggatatactg atttagctct
    12361 ccagcagtgg cagttgctgc tggagatgac aaaaggcaat gtgcttgtcc agaacaagat
    12421 agatatgatt aatttcagaa gctcactgta caatgggctt gannnnnnnn nnnnnnnnnn
    12481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnccgtgacc gctatggcag accaatctat ggtttaacac
    12601 aggataatta tacagtcgtt gaagatggag caactatcag cacattaaat gttgattact
    12661 taaagaacaa gatgcctgcc gcatcgatgg tactatgtgt ggatcgttca caacctttgc
    12721 agcggtacca taatgatata ccatggcttg ctgagtttat attaaaaaaa atgcataaaa
    12781 atgataaact taaaatactt aattttgccg ctgatacatg ggttgccaat ccctatgact
    12841 ggagcaggcg tcgtgcgctg aaagcattaa aaaattttga ctacgacagc gggcgtgata
    12901 ttggcaaagc gctgtatatg gcaataagtg atgtattgcc ccagatggaa cggcgaggag
    12961 taatactcat tactgacggt gacactgctg aaaaagattt taaggcatat tcagcggata
    13021 tcattattga ttatgcaaaa tcgcatttta ttccagtata tatattaaca ttaaaaacaa
    13081 aatctccatt gcttatgcgc attgccaatg aaacgggagg agccatatat aagggaagcg
    13141 agctggatgg tttacgcaca gtgtatgatg caataaaaaa ttccaatgac tatcgctatg
    13201 ttttattgta ccatactttt aagatgaaat cgttaacagg ctggtggtcg gatgttacga
    13261 taaatgtatc atataaaggg caaaaaggaa ctgaatgggc aggatatttt gtaccataaa
    13321 aactatcgct aacaaagctt aaatgtgctc aaatattgaa ataaaatata gaaaaatagt
    13381 tgacaaaata catggtttgt tgttttgtaa caagcagtaa gtgtgaaata aataaataaa
    13441 cttttttact ggttgtcatg attttttctc tacaaaaggg cgtgaagtaa taataaacaa
    13501 tatttagctg tctctgtgac agcagtttat agtggaattg tataaagata tgagagggga
    13561 ttttctgtgg ctcagttaca acatatatat agtatatata aatataccaa aagcatagat
    13621 actatattta caagttttta ttatataaaa ataataacaa accatagtga tacatcaaat
    13681 cataatacag gtaacgccat gcattgtgca ttggtaatat actattattt aatgatggag
    13741 gatttctttt atgaacaatc aaactaaaag ctttttaaca acatgcgtat ttctgtttat
    13801 gataatcatg ttaaatgggt gtgtaacgtc atcatatttt acattaacag aaaatcaaaa
    13861 gccacccatt gtacctgata caatagctgt tattagcggg agtgcctcag agattgatat
    13921 tcatttagca aataaattaa ccgaggaact gtccaatact aaatttaaag tcatgagcca
    13981 ggagcaaatt acaaaaataa tccccgacta tccattgaat atcatcaact ttacatgtga
    14041 aggcaagaaa tttaaacaac catatatatc ggatgaatca aaaaaaatag tggatgcaat
    14101 gcaatctaaa ctaaaggtaa aatacgtttt attgatatgg aataatggag tcggtacggt
    14161 gacatatacc aatcgatatg ggcaacaatc cgggaaggaa gtagaggtgt gtatattaac
    14221 acgttttctt aaataccctg aagctacaat agttggatat tcattttttt gtgattcaaa
    14281 aagtttggga tatttcttta tgataaatga ggataaaaaa atagctaaaa ccgttgaaga
    14341 gttattaaac aaaggggtaa aaaagattgt taaagaaatt gtaaagaata ctgaaaatcc
    14401 cgcaggtaaa aagtaaaatt atgcaaggaa ctaaattttt ctgttctatc ataatgatat
    14461 tgctgttccc tgtatattct ggtagtatct atgcttcaga gtataatgag caatactctg
    14521 aagcatttac tttaaaagtg cacctcatca attatgatca aattgttaca atacaggata
    14581 aaaataataa aggaaa
//
LOCUS       DAOOJH010000060        14394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4018_length_14394_cov_8.636516, whole genome shotgun sequence.
ACCESSION   DAOOJH010000060 DAOOJH010000000
VERSION     DAOOJH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4018_length_14394_cov_8.636516"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /gene="recD"
                     /locus_tag="PLJ75_08500"
     CDS             <1..1021
                     /gene="recD"
                     /locus_tag="PLJ75_08500"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPD05291.1"
                     /translation="RLPYDVVIVDESSMVDIALLAKLIKALKEGARFIVVGDKDQLSS
                     VEAGAFFGDLCWGVTQFGYTSQWNKTVAAIKDDDAQQLPSHPVRDAIVVLSKAYRFGA
                     DSGIAVLAQAVRDADMHKVDNVLKNSYNDVEFIQYSSIDEIHTIIKEYAKQHYESVAI
                     AHNVSDAFSKISHQCIVTPFNQGPWGVSTLNAIIEKQINPLASGWYHGKIIMVTANDY
                     NLQLFNGDMGIFVNNDDGESGVCFPLEDSYRKINPLLLSEYIPAYTITIHKSQGSEFN
                     TVIVVLPNDINSRWSMLLTRELLYTAITRAKEKLIIYGTHDVIAYMVNNPTKRASGIQ
                     GKVWQ"
     gene            1209..2165
                     /locus_tag="PLJ75_08505"
     CDS             1209..2165
                     /locus_tag="PLJ75_08505"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPD05292.1"
                     /translation="MKVLIYGGGAVGLGLARALVDSGCATTIIDIEPTVTALRTHGII
                     QSGVLGDFAINPERFTVYDSILKADDNYDYMCVCIKSYLSEATAQDIASHYTHKQTPI
                     VLFQNGWGNAEKFISYFDKERIFNARVMTGFMKQELYHVIITVHADAIRMGSLYHGYG
                     KRLLSLAEALTTGGMAAEVVNDVVKYIWAKMLYNCALNPLGALFNVEYGDLGKSEYTK
                     AIMDDIITEIFETMHRAGYTTHFSNAAEYINVFYEKLLPPTAKHRSSMLQDIQAKRKT
                     EIDALNGAVVALAQHYGYEAKVNGIITGLIKFIESTYTGATC"
     gene            complement(2431..3672)
                     /locus_tag="PLJ75_08510"
     CDS             complement(2431..3672)
                     /locus_tag="PLJ75_08510"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPD05293.1"
                     /translation="MKRLLWCLSTILTCLTIISFAIAQTDFQPTQGKITASTVNIRNI
                     PSAGGVNIGTLKRSDVVNVIGRSQNKSEIEGKEDYWYKIQFNDGKKTITGWVFGGFIS
                     FELNLESGLRWKSATPSPGASLTAISTDTNGNIYAGTSSGDLFISFDYGKSWRKVVPQ
                     ALGVSIGHIKKIVIDDNMILIASKGDKNGGIWKSTNNGASWSQFTTSQGLPSNEVVDI
                     AIAPNKYIYAITSDKICVSNDGCANWKILDDSGLKGKYNVIAIDRSGIVFVGTNKGAF
                     KMKKLIDVFGKEDTSWIAVGQKSPNMGEVYAIAINPHANEIYVGCNKGLNKSNLDNLD
                     SWQGIGGQSIINDIMIEPDGRTSVATEYGLNISIDNGASWVTYKSENGLSNNYVYRIA
                     LHSKNKSIWLITGKDGINYSE"
     gene            complement(3694..4650)
                     /locus_tag="PLJ75_08515"
     CDS             complement(3694..4650)
                     /locus_tag="PLJ75_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPD05294.1"
                     /translation="MKITRFFVSLLLIACLVPFVACKGKTKTILNEVYLIQNPEDKKI
                     AYVPNSKLKRGEFVKVIDEKVVNQKKYYYVQIEDVDVKGWIAENYVHDGKLESVTVLT
                     DTDLYMRPSIKSDKIGKVFAGQIAFKIDKKGDFVLIQYPGKEGYVLKDKVGTGEIIIK
                     KVTIPGIGSATVNASSQLVLGEGRELEFDPRNAFDGSLQTAWCEGKSGDTGVGEWIQI
                     SFDQCINLEEVSIVNGWSKSEDLYKTNTRVAQLKITSNKGGEAVVELSDGIFDFQKFD
                     ISLKGSSFKFIITKVYPGKDSDTCISEISLKSSSNQCEDYYD"
     gene            4868..6013
                     /locus_tag="PLJ75_08520"
     CDS             4868..6013
                     /locus_tag="PLJ75_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD05295.1"
                     /translation="MTPIISSLLSVMLIFTQVPQRSVAGKEYIICGNNTQQKIYYTTS
                     SNYSKNHKGEKAIDNDTVTSWMSQHDGDQWIEIDFVVKRVITDIKVVAGYRDNYQTIT
                     YFILQFMYDGKWFDFARVDFTRKEKKGLFGTHSVYNPVAVAALGGIDASTFRIYIPQD
                     AIVNGYAAIAEIEVYAGSGKLQYFDKRLYNLCFPIKNGLFPENDAGYPNSPRGYRGGI
                     HYGLDILYYFNEDTYQPIPLDETTPVLACADGVVIRADYDYKPPAEEEWKKLSLYYQG
                     NPATFVQRSFGGRQIWIDHQNGVLSTYNHLSKIDNKVSAGSRVKKGQRIGWVGNSGLL
                     GEAQGKKWGQHLHFELWVDGIYLGYNMNINEIKRYLRWIFAIRDMEE"
     gene            6016..7185
                     /locus_tag="PLJ75_08525"
     CDS             6016..7185
                     /locus_tag="PLJ75_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05296.1"
                     /translation="MTDIKKRAYQFIIVFGIISLLGDIIYEGARSVNAQYLKGLGANA
                     LWVGIVAGLGEFVGYAIRLVSGYLSDKTRAYWLFTFIGYGLLITVPLLALTGIWQVAA
                     LLMIGERLGKALRSPAKDTILSQVTTQVGRGLGFGIHEAMDQIGAIAGPLMFTFIFAY
                     VVDYNLGYKIMWIPFILLMGVLIIAKMLVPHPEQFESTPHSNSSDALTPTFWLYNAFS
                     AFAIIGFASFPIIAFHLKSQNIVSDMAIPLLYAIAMGVDAVFAIIIGRLYDKKGLSVL
                     LVIPIASAIVPVMAFSFHWVAVTIGIILWGLVMAVHETIMRAAIADLTSLKKRGTGYG
                     IFNTAYGLCMFIGATAMGWLYEIDMRYIMAFSFINELIAVVLFVYIIKSIHSQMK"
     gene            7280..7642
                     /locus_tag="PLJ75_08530"
     CDS             7280..7642
                     /locus_tag="PLJ75_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019951681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05297.1"
                     /translation="MALEQKDMEFIEHYLREHLPRILAEQSLAKPPVVYEIELRERMV
                     RVEEELKHQRELMQKGFELMEKSFQMMERRFEQIDKRLELLENRFDNLLKRLFQFMIW
                     TFGMIISATGIIIAFMKM"
     gene            7695..8465
                     /gene="xth"
                     /locus_tag="PLJ75_08535"
     CDS             7695..8465
                     /gene="xth"
                     /locus_tag="PLJ75_08535"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPD05298.1"
                     /translation="MKLKLISWNVNGIRAVYKKGFYDWFINNNADIVCLQETKATEEQ
                     FPEELLKIPHYHKYFSTAEKKGYSGVALFTRIQPVKVEKGFGIQCFDNEGRILIAYYD
                     AFVLYTIYFPNGKASKERLQYKMDFYDAFLEHAENVRKNGHSIIMCGDVNTAHTELDL
                     ARPKENETVSGFLPQERAWIDKFISYGYVDAFRLFVKEGGHYSWWDYKTKARERNIGW
                     RIDYFFISEDLKTKVKDAFIQKDIEGSDHCPVGIVIDL"
     gene            8849..9034
                     /locus_tag="PLJ75_08540"
     CDS             8849..9034
                     /locus_tag="PLJ75_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPD05299.1"
                     /translation="MPVYEFKCKHCNAVFSELRKIGDTKAPECPDCHSDDTEKIFSVF
                     SSGAGVKPSCASSSGGG"
     gene            9665..9943
                     /locus_tag="PLJ75_08545"
     CDS             9665..9943
                     /locus_tag="PLJ75_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05300.1"
                     /translation="MKRTIAIVIVFLCIPPLYAEKKNYCELMRELVINISKEAKKHNP
                     EFIIIGQNALELLTINGKPTGKLANNYCHAMDAIGIEELFWGYSLNKR"
     gene            9903..10268
                     /locus_tag="PLJ75_08550"
     CDS             9903..10268
                     /locus_tag="PLJ75_08550"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HPD05301.1"
                     /translation="MKSFSGGIPLTNDDVHRLQKKPNGARRLVIAYMSVGEAEDYRYY
                     WKAGWEKSKPQFLEQENKLWKGNYKVRYWDKQWHVILYGNGNEELFGDSYPGRVIAAG
                     FDGVYMDVLDAAHYFQEKK"
     gene            10372..12411
                     /locus_tag="PLJ75_08555"
     CDS             10372..12411
                     /locus_tag="PLJ75_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05302.1"
                     /translation="MGSLKGKFKSLKVILSTIVGLIVTTAIIILIIISYNAAYSELEK
                     SYVNQIQNVNSGINMLVEQYFAQNIQVAELYASRDDVRESLVMGNFGPVTKELTAFLN
                     KYKELYENIFIATADSNPVILAAGGPGAVGTRYRNASYARAIDEALQGKVGLSKPDKS
                     PVTGQPVILIQVPIRENGRIVGLLGLPLELGKYSYKLVKDVKIGYTGYPYVTDIDGLV
                     FAHPEKENILKLEMSKLDFGQKLLSSPDNTVIKYKWEGKDKMLVGEKNKGYGFIVAST
                     MYMSDIANSARDMATTMVLFGLIMLAGAVIGIYYIIASRLNPLEKLKTIAGSLAEGNV
                     TMRYEGRVYSDEIGEMSTAINNTMENLEKLVAEVKVAVSNLTQAVNEIASGNENLSQR
                     TSEQASSLEEIAATIEQATATIKQNADHASRANQIAEKTSELAKNGNTLVGDAVVAIN
                     EINASSKRIGEILTLINEISFQTNLLALNAAVEAARAGEQGRGFAVVAGEVRNLAQRS
                     GSAAKEIGELIRDSLDKIDTGTTLVNKSGEALKEIMQSIQDLYKTITEIATASDEQKQ
                     GIDQINVAISDLDTMTQQNAALVEETASASEEMANQAQELADMMERFVIRDEVSQKIY
                     GTKHKELHLHAADSAKKPVTKKVDKQVKSKEEKKEEEKKEEGKNNKLIDDGFEEF"
     gene            12437..>14394
                     /locus_tag="PLJ75_08560"
     CDS             12437..>14394
                     /locus_tag="PLJ75_08560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPD05303.1"
                     /translation="MAEDAIRQVFLAETKELLENLENDIVRYEETKDPELIHSIFRYV
                     HTLKGGSGMAGYDDVYQFTHALENVLDSVRNGIIPMDDLLADIVFASIDVIRLRLYTD
                     SDQQQMLEKFNLLQSKINDIETLLQKKEEAEPAEEPEEKKFIYNFFRVKAKFRDDIFK
                     FGIDPLIIMEDLSSLGKIVELRVNRNNVPLLQDIDPEQCYIAWDVILKTKSKAEALDD
                     VFIFVRDDNEITITDVTKNYVAEQVIEDVPKLGELLLSRGMLTENEFEDVLNEHEKTK
                     KKVGEIAVTKGYIEENDVSLALKEQDEIKKQISTSTLRVDAKKVDNLMNLLGELVIGF
                     SGIKRIAEEIEDEKAYRLNNALYSVDRIIREFQEHLMRVRMVPIGPIFEQYRRFIRDT
                     AKAHGKSIRLEITGGDTEIDKTVIERISDPLKHLIRNAIDHGIEAPHDREEKGKPREG
                     LVSLKAYHQEGNMYIEVSDDGAGIDKEKVIEKAVAKGLLSKNDEIPDSTLYGFLFEPG
                     FSTSESVGDLSGRGVGLDVVKTNIEALRGTVDILSQPEKGTTFIIKLPLTLAIIDGML
                     VRVAHETFIIPILSIIESIKPVKDDIKTIESQGEVVHIRGEYVPLLHLHQEFNLPQTI
                     QNPWDGLVVVVESQGRSIGLIVDELIGQ"
BASE COUNT         4566 a         2368 c         3023 g         4437 t
ORIGIN      
        1 tcgtcttccc tatgatgttg tgattgttga tgaatcatcc atggtggata ttgctttact
       61 cgcaaaactt atcaaggcat taaaagaagg tgcacgtttc attgtagtag gtgataagga
      121 ccagctttca tcggttgagg cgggtgcgtt ctttggcgat ttatgctggg gtgttaccca
      181 atttggctat acatcacagt ggaataaaac agtagcagct ataaaagatg atgatgcgca
      241 acagttgcca tcacatcctg tgcgcgatgc aattgttgtg ctttctaaag cttatagatt
      301 tggtgcggat agcgggatag cagtattggc gcaggctgtt cgcgatgctg atatgcacaa
      361 ggttgacaat gttttaaaaa atagctataa cgatgtggag tttatacaat acagttcaat
      421 tgatgaaata cataccatta tcaaagagta tgcaaagcag cattatgaat ctgttgccat
      481 agcacacaat gtcagtgatg cttttagtaa aatatcccat caatgtatag taacaccgtt
      541 taatcagggt ccgtggggtg tatcaacgct gaacgcaata attgaaaagc aaataaaccc
      601 ccttgcttca gggtggtatc atggcaagat tattatggtt accgccaatg attataatct
      661 acaactgttc aatggtgata tgggaatatt tgttaataat gatgatggtg aaagcggggt
      721 gtgcttccca ttagaagata gttaccggaa gattaatcct ttgctgttat ccgaatatat
      781 cccggcgtat acaataacaa tacataaaag tcagggttct gaatttaata ctgtgatagt
      841 agtgctgccc aatgacatta atagcaggtg gtcaatgctt ttaacacgag agcttttata
      901 tactgctatt accagagcaa aagaaaagct tattatatat ggtactcatg atgttattgc
      961 atacatggtt aataatccta caaaacgtgc ttcaggtata cagggaaagg tatggcaata
     1021 aatgtggtaa taaaaaagtc atgaaacaac ttaataattg ctatcagatt atactttttt
     1081 agtattgact ggttcaactc atggtacaac aatggtgcga tagttactaa agtaacaggt
     1141 tatgtgctta gaataacttt gatgctgaat atacggcaaa tgtgcctgca taatcaagga
     1201 aggttgctat gaaggtgtta atctatggcg gtggtgctgt tggattgggt ctggcgcgag
     1261 cgcttgttga tagcggttgt gcaacaacca ttatagatat tgaaccaacg gtaacggcgt
     1321 taaggacgca tggtatcata cagagcggag tactgggtga tttcgcaatc aaccctgaaa
     1381 ggtttaccgt atacgatagt atcctgaaag cagatgataa ctatgattat atgtgtgttt
     1441 gtattaaatc atatttatct gaagctacgg cgcaggatat tgcttcccat tatactcata
     1501 aacaaacgcc tattgttttg tttcagaatg gatggggaaa tgcagaaaaa tttataagct
     1561 atttcgataa agagagaatc tttaatgcac gtgtcatgac cggatttatg aagcaggagc
     1621 tatatcatgt gattataacg gttcatgctg atgccattcg tatgggaagc ctctatcatg
     1681 gatatggtaa gagattacta tcgttagcag aggcgctcac tacaggtgga atggcagcag
     1741 aagttgtcaa tgatgtggta aagtatatat gggctaagat gctttacaat tgtgcgctta
     1801 atcccctggg tgcactcttc aatgtagagt acggtgatct tggtaaatca gaatacacaa
     1861 aagcaataat ggatgacatc ataaccgaga tctttgaaac aatgcacagg gctggataca
     1921 ccacgcattt cagcaatgca gcggaatata ttaatgtgtt ctatgaaaag cttttgccac
     1981 caacagcaaa gcatcgttca tccatgcttc aggatattca ggcaaaacga aaaaccgaga
     2041 ttgatgccct caacggcgca gttgttgcat tagcacaaca ctatggatat gaggctaaag
     2101 taaatggaat tatcacaggt ctgataaaat ttattgagag tacctatacc ggcgctacct
     2161 gttagcgtta cgcttattgc tttatcgaaa gtcctgctaa tgcagtaaca acattttttg
     2221 ctaacttcta tgacaaaatc attttcagaa tatagaattg ttcaccacta ccatcattcc
     2281 tgatgatgga atcttttttt aggttgtcaa tgattatagt actgtcatcc tatgtaaaaa
     2341 tagtaaagca accatcacgg ttgctttact attatctgtt gtattaatat gtattaataa
     2401 cgcaaaataa attaaacaag cagggaaata tcattctgaa tagtttatcc catctttgcc
     2461 ggtaattagc cagatacttt tattcttgct atggagagct atcctgtaaa catagttatt
     2521 tgacaatcca ttttcagatt tgtaggtaac ccacgatgca ccattatcta ttgatatatt
     2581 taaaccatat tcagtagcaa cacttgttct accgtcgggt tcaatcataa tatcatttat
     2641 aattgattgt ccacctattc cctgccagct atcaagatta tctaaatttg acttgtttaa
     2701 acctttattg catccaacat aaatctcatt agcatgtggg ttaattgcta tggcatatac
     2761 ttcgcccata ttaggtgatt tttgtcccac tgctatccat gaagtatctt ctttaccaaa
     2821 tacatcaatt aactttttca tcttgaaagc gcccttatta gttccaacaa atactatacc
     2881 tgacctgtca attgcaataa cattatattt cccctttaaa ccgctgtcgt ccagtatttt
     2941 ccaattagca cagccatcgt tacttacaca tattttatcg ctggtaatag cataaatata
     3001 tttattagga gcaattgcta tatcaaccac ctcattagaa ggtaaaccct gtgatgtggt
     3061 aaactgtgac cacgatgcac cattgtttgt tgacttccag atacctccat ttttatcccc
     3121 tttagaagca attaaaatca tgttatcgtc tataactatt tttttgatat gccctatcga
     3181 tacacccaat gcctgaggta ctacttttct ccacgatttt ccgtaatcaa atgaaataaa
     3241 gaggtccccc gatgatgttc ctgcataaat gttaccattg gtatccgtag aaattgctgt
     3301 taaagaagct cctggagatg gagtagcgct tttccatcgc aaaccacttt caagatttaa
     3361 ctcaaacgat ataaatccac caaataccca ccctgtaata gtttttttac catcattaaa
     3421 ctgaatcttg taccaatagt cttctttacc ttcaatttct gatttattct gtgaacgtcc
     3481 tataacatta acaacatcgc ttctctttaa agtgccaata tttacccctc cagcagatgg
     3541 tatattacga atgttgactg tactggctgt tattttaccc tgggtaggtt gaaaatccgt
     3601 ctgggctata gcaaatgata tgatcgttaa acatgttagt atcgttgaca agcaccataa
     3661 aagtcttttc attattcccc tccatttgta atattaatca taataatctt cacattgatt
     3721 tgatgaactt ttgagtgata tttcacttat acatgtgtca gaatctttac ccggatatac
     3781 tttagtaata atgaatttaa atgatgatcc ttttaagcta atatcaaatt tttgaaaatc
     3841 aaaaatccca tcagataatt caaccactgc ttcgccacct ttattagatg taatctttaa
     3901 ctgtgctact cgtgtattgg ttttatataa gtcttcactc tttgaccaac cgttaactat
     3961 gcttacctct tcaagattta tacattgatc aaacgaaatt tgtatccatt cgcctacccc
     4021 cgtatctccg gatttccctt cacaccatgc ggtctgcaag ctgccatcaa aagcatttct
     4081 tggatcaaat tcaagctctc gaccttcacc taaaactaat tgtgatgatg cattgacggt
     4141 tgcacttcca attccaggga tagttacttt ctttataata atttctccag ttccaacttt
     4201 atctttgagc acatatcctt cttttccagg atattggata aggacaaaat cacctttttt
     4261 atctatttta aatgcaatct gtccggcaaa gactttgcca attttatcac ttttgataga
     4321 cggcctcata taaaggtcgg tatcggtaag tacagttaca gattcaagtt ttccgtcatg
     4381 tacataattc tcagcaatcc agcctttgac atcaacatct tctatctgga catagtaata
     4441 ctttttttgg tttactactt tttcatcaat gactttaaca aattcacctc tttttaattt
     4501 actgttgggg acataagcaa tttttttgtc ctcaggattc tggattaaat aaacctcgtt
     4561 taagattgtt tttgtttttc ctttacatgc tacaaacggt accaaacaag ctattaatag
     4621 taatgaaaca aaaaatctgg taatcttcat ggacgtctcc ttttgttaat tttagtatta
     4681 ttgtttatca ggattttatt aacatttgtt taatagttac aaattatttg tcaattaaaa
     4741 ttttatattt tccttttttt cgtaatctgt acataaactt tttttataaa aaagtgctgg
     4801 attttagcaa agttgtatta gcatgtatgt catttattta caattttaaa gtaaagaatg
     4861 atgatatatg acaccaataa tttcttcact actttcggtt atgcttattt ttactcaggt
     4921 tccccaaaga agtgtagcag ggaaagagta tattatctgc ggcaataaca cccagcaaaa
     4981 aatatattat accaccagca gcaactatag caaaaatcat aaaggtgaaa aagcaattga
     5041 caatgatacg gtaacatcgt ggatgtcaca gcatgatggc gatcagtgga tagaaataga
     5101 cttcgtcgtt aaacgcgtga taactgatat aaaggttgtt gcaggttata gagataacta
     5161 tcaaacaatt acatacttca ttttgcagtt tatgtatgat ggtaaatggt ttgattttgc
     5221 cagggttgat tttaccagaa aagaaaaaaa aggattattt ggcacacata gtgtctataa
     5281 tccggtggcg gttgctgcac tgggtggtat agatgccagc acattcagaa tatatatccc
     5341 acaggatgcg atagttaatg gatatgctgc cattgccgag attgaggtgt atgcaggtag
     5401 tggaaaacta cagtattttg acaagaggtt atataatctg tgtttcccta ttaaaaatgg
     5461 gttgttccct gaaaacgatg ctggctatcc caattcgcca cgagggtacc gtggcggcat
     5521 acattacggt cttgacatat tatattattt caatgaggat acctatcagc caatcccgct
     5581 ggatgaaaca acaccggttt tagcatgtgc tgatggagtt gtcatacgag cagattatga
     5641 ttataaacca ccagcagaag aggaatggaa aaagctttcg ctgtattatc aaggcaatcc
     5701 tgcaacattt gtacaacgat catttggtgg cagacaaatc tggatagatc accaaaatgg
     5761 ggtattatct acctataatc atctttcaaa gattgataat aaagtaagtg ctggcagcag
     5821 ggtaaaaaaa ggtcaacgca ttggatgggt gggaaattca ggactgttag gcgaagctca
     5881 gggcaaaaaa tgggggcagc accttcactt tgagctgtgg gtggatggta tttatttagg
     5941 gtacaatatg aacattaatg aaataaaacg ttatttgcga tggatatttg caattcgtga
     6001 tatggaggaa taacaatgac cgatataaaa aagcgtgcat accagtttat tattgttttt
     6061 ggcataataa gccttctggg cgacattatc tacgaaggtg ctcgcagtgt taatgcacaa
     6121 tatttaaagg ggctgggtgc caatgcgctg tgggttggca ttgttgcagg attgggggaa
     6181 tttgtaggct atgcaatacg gcttgtttca ggatacttaa gcgataaaac aagagcatac
     6241 tggctattta cctttatagg gtatgggctg ctcatcacgg ttcctttgct ggcattaacc
     6301 ggcatatggc aggttgcagc tcttttgatg attggtgagc gtttaggaaa ggcgttgcga
     6361 agccctgcaa aggatacgat tctgtcacag gtaacaactc aggttggcag agggttgggg
     6421 tttggcatac atgaagcaat ggatcagatt ggtgctatag ccggtccgct gatgtttacg
     6481 tttatctttg cctatgtggt tgattacaac ctggggtata aaatcatgtg gataccattt
     6541 attttgctta tgggagtttt gatcattgca aaaatgctgg taccacatcc tgaacagttt
     6601 gaaagcacac cgcacagtaa ttcgtcggat gctctgaccc caacattctg gctatacaat
     6661 gcgttttctg catttgcaat catagggttt gccagtttcc ccattattgc ctttcactta
     6721 aaaagtcaaa atattgtcag cgatatggcc atacctttgc tgtatgcgat agcaatgggg
     6781 gttgatgctg tctttgccat aattattggc agattgtatg ataaaaaggg actatcagta
     6841 ctattagtta taccaatagc ttcggcgata gttccggtaa tggcttttag ttttcattgg
     6901 gttgcagtga cgattgggat tatactgtgg ggacttgtga tggcagtaca tgagacgatc
     6961 atgcgtgcag cgattgctga tttgacatca ctgaaaaaac gcggtaccgg ctatggcata
     7021 ttcaataccg cctatgggct ctgcatgttt attggtgcaa cagcaatggg ctggttgtat
     7081 gagattgata tgcgctacat tatggctttt tcgttcatca atgaattgat tgccgtggtg
     7141 ctttttgtat acattataaa atcaatacat tcacagatga aataatgtat tgacattact
     7201 atgattatgt actacattat tatacaaaag caatatatag tatatgtttt tgctaatttt
     7261 gtaattaagg gatggtaata tggcattaga gcaaaaggac atggagttta tagaacatta
     7321 tttaagagaa catttacccc gtatcctggc tgagcagagt ctggcaaaac cgccggtggt
     7381 atatgagata gagcttcgtg aacggatggt gcgtgttgaa gaagagttga aacatcagcg
     7441 tgagttgatg caaaagggtt ttgaattgat ggaaaaaagt tttcagatga tggaaagacg
     7501 atttgagcag atagataaac ggttagaatt gctggaaaat agatttgata atcttttaaa
     7561 gcgattattc cagtttatga tatggacatt tggaatgatc atatcagcta caggaattat
     7621 tattgcattt atgaagatgt agtggttgag gtaatgcact gctgctactg catatataaa
     7681 agagaaaaaa caatatgaaa ttaaaactta tttcgtggaa cgtcaatggg attagagctg
     7741 tttataaaaa agggttttac gattggttca tcaacaacaa tgcggatatt gtatgcttgc
     7801 aggaaacaaa ggcaacagaa gaacaatttc ccgaagagct tttaaaaatt cctcattatc
     7861 acaaatattt ttcaactgcc gaaaaaaaag ggtatagtgg tgttgcttta tttaccagga
     7921 tacagccggt taaagttgaa aaaggatttg gcattcaatg ttttgataat gaaggacgca
     7981 tactcatagc gtactatgat gcgtttgttt tgtatactat ctatttcccc aatggcaaag
     8041 catcaaagga gcgcctgcaa tataagatgg atttttacga tgcattttta gaacatgctg
     8101 aaaatgtgcg caaaaatggt catagcatta tcatgtgtgg tgatgttaac acggcccata
     8161 ctgaacttga tttagcccgt ccaaaagaaa atgaaaccgt ttcaggattt ttaccacagg
     8221 aacgtgcatg gatcgataag tttatatcct atggttatgt tgatgcattt cgtttatttg
     8281 taaaagaagg tgggcactac tcatggtggg attacaaaac aaaggctcgc gagcgtaata
     8341 taggatggag gattgactat ttctttatca gtgaagattt aaaaacaaag gtaaaagatg
     8401 catttataca aaaagatatt gaaggttcag atcattgccc tgtgggtatt gttatcgacc
     8461 tataatcgat agagcgcttt tttttggtag ctccattgtc acagcctttc catgcacttt
     8521 gcttgatgct attataactg cgattccatg ttttaagcga taattccatt atgcacatcc
     8581 tttatcttat acagaattaa tatgctgtta atataaataa aaaatgtttt agccaattta
     8641 ttgttactgt aaattttaaa aatgcttaat aaaaaatata caaaacaatt atgggcgata
     8701 gttactgatg aacggtttta ttttctaaaa aattttattg aaaccaattg aatgttagta
     8761 tatagtaaaa tactatacaa cctgttttgt gttatggaag ctacattact tattatttca
     8821 tttcattata acagtagagg attattatat gccagtatat gaatttaaat gtaagcactg
     8881 caatgctgtt ttcagcgaat tgcgaaagat aggtgataca aaggcgcctg aatgtccaga
     8941 ctgccattca gatgatactg aaaaaatatt ctcggtattt tcaagtggtg ccggggtaaa
     9001 accatcatgt gcatcatcat cgggcggtgg atgaggtata taatctgagc cagtttggct
     9061 ctttataagg ctctgacccc aatgtgtcaa taacagtact tattcatgct caacgtgttg
     9121 tactgcacta ttttaagtgt cagcttctat agtttctttt atcttttcta ctgattcatt
     9181 aagggtagca atagatagtt caccaattat attttgtttt tctgatagct cattcaatat
     9241 gttttagctt gcttactatt ggcaggtttt gacgtatctt ttagtaagtt aagggctttt
     9301 tcataacttg ctgcatcaac aactaaactg atatcatcat tataacctgt atttaccaac
     9361 ttgtcttcaa cccatttact atctggtaaa tgaccaataa aaatctcaac ccagccatct
     9421 ttttctgagg taataaatgc tatagttagt ataattttat ttttaaataa gcatatatgc
     9481 aattgaattg attgtcaaga aaaaacgggt ggtgaaacgc tatgctaatc aaacgctgta
     9541 aaaaagggtc agaggggtca gagcctatgt acagttaatc ccgtaaaaaa tttcttgcat
     9601 atattattga atcgattatc attttatttt gcactttgat gatagtgaaa gggtatgcca
     9661 ctctatgaaa aggactattg ctattgtcat agttttttta tgtatccctc ctctttatgc
     9721 ggaaaagaaa aattactgtg agcttatgcg tgaacttgtt attaacatta gtaaggaagc
     9781 aaaaaaacat aatcctgaat ttatcatcat tggacagaat gcgctggagc tgcttaccat
     9841 taatggtaaa ccaacgggca agcttgcaaa caattattgt catgcaatgg atgcaatcgg
     9901 cattgaagag cttttctggg ggtattccct taacaaacga tgatgttcac aggttgcaga
     9961 aaaaaccaaa cggggcgcgc aggttggtga ttgcctatat gtcggttggc gaggcggaag
    10021 attaccgcta ttactggaaa gccgggtggg aaaagtcaaa gccgcaattt ctggaacagg
    10081 aaaataagct atggaaaggc aactataagg tacgatactg ggataaacaa tggcatgtaa
    10141 ttttatatgg aaatggcaac gaagaacttt ttggcgatag ttacccggga agggttattg
    10201 ccgctggatt tgatggagta tatatggatg tacttgatgc tgcacattat tttcaggaga
    10261 aaaaataaat tttttgattt tttcaaactg attaattata tttgaggaag gctttttata
    10321 atttaaatat gtatcacaac ttgattccac aataatagca aggagggcgt tatgggttcg
    10381 ctaaagggta agtttaaaag cttaaaggta atactttcca cgattgttgg attgattgta
    10441 acaacggcga ttattatttt aattattata tcatataatg ctgcatattc tgagctggag
    10501 aagtcgtatg tcaatcagat ccaaaatgtt aatagtggta ttaatatgct ggtagagcaa
    10561 tactttgctc aaaatattca ggttgcagag ctatatgcat caagggatga tgtccgtgaa
    10621 tcgcttgtaa tgggtaactt tgggccggta acaaaggaac tgactgcatt ccttaacaaa
    10681 tataaagagt tgtatgaaaa tatatttata gcaactgccg atagcaatcc agtgatactt
    10741 gctgcaggtg gtcccggcgc agttggtaca cgctatagaa atgccagcta tgcaagggca
    10801 attgatgaag cattgcaggg taaagtagga ttaagcaagc ccgacaaatc accggtgacg
    10861 ggacagccgg ttatccttat acaggtgcca attagggaaa atggcaggat agttggatta
    10921 ttgggactgc cacttgaatt gggcaaatat tcatataaat tagtaaagga tgtaaagatt
    10981 ggatataccg gctaccccta tgtcactgat attgatggat tggtatttgc tcatccggaa
    11041 aaggaaaaca tattaaagct ggagatgtct aagctggatt ttggacaaaa gttgttaagc
    11101 agccctgata ataccgtcat taagtataaa tgggaaggga aagataaaat gcttgtcggt
    11161 gaaaaaaata agggatatgg ttttattgtg gcaagtacca tgtacatgtc cgatatcgca
    11221 aattctgccc gtgatatggc tacaaccatg gtgctttttg gactgatcat gttagctggt
    11281 gcagtaatag gtatttacta tatcattgcc tcgcgtctca atcctctgga aaaattaaaa
    11341 actatagccg gttcacttgc cgaaggaaat gttaccatgc gctatgaagg cagggtatac
    11401 agcgatgaaa ttggtgagat gtctaccgct attaacaata ctatggaaaa tttagaaaag
    11461 cttgtagccg aggtaaaggt agcggtaagc aatttaacgc aggcggttaa tgagatagcc
    11521 agcggcaatg aaaacttatc gcagcgtacc agcgaacagg cttcatcact ggaggaaatt
    11581 gccgcaacaa tagaacaggc tactgcaact atcaagcaaa atgcggatca tgcatcaaga
    11641 gcaaatcaga tagcggaaaa gacaagtgag ctggcaaaaa atggcaatac gctggttggc
    11701 gatgcagtag tggcaattaa tgaaataaat gcatccagca agcggatagg cgagatactt
    11761 acgcttatca atgagatttc attccagaca aacctgcttg cgttaaatgc agccgttgaa
    11821 gctgcacgag cgggagagca gggcagaggt tttgcggtgg ttgctggcga agtgcgtaat
    11881 ttagcacaac gctcaggcag tgccgctaag gaaataggtg agcttattcg cgattcatta
    11941 gataagattg ataccggtac aacactggta aacaaaagtg gtgaggcgtt gaaggagatt
    12001 atgcagtcta tacaggattt gtataaaaca ataactgaga ttgcaacagc aagtgatgag
    12061 cagaagcagg gaatagatca gataaatgta gcaatatcgg atctggatac gatgactcag
    12121 cagaatgcag ccctggtaga agagacagca agtgcaagcg aagagatggc aaatcaggca
    12181 caagaattgg cagatatgat ggaacgtttt gtgattagag atgaggtaag tcagaagatt
    12241 tatgggacaa aacataaaga attacatctt catgctgctg attcagcaaa aaaacctgtg
    12301 acaaaaaagg tagataagca ggtaaaatca aaagaggaga agaaagaaga agaaaaaaag
    12361 gaagaaggca aaaacaataa gcttattgat gatggatttg aagaatttta aattgaacta
    12421 tagtgcgagg tgaatcatgg cagaggatgc catacggcag gtatttctgg cagagacaaa
    12481 agagttgctg gaaaaccttg aaaatgatat agtacgctat gaagaaacta aagatcctga
    12541 attgatacac tctattttca ggtatgtgca tacgttgaaa ggtggatcag ggatggccgg
    12601 atatgatgat gtgtatcaat ttacccatgc tcttgaaaat gttcttgatt cagtacgcaa
    12661 tggtatcata cccatggacg atttgcttgc agatattgtg tttgccagta tagatgtcat
    12721 ccgcttgcgt ttatataccg atagtgacca gcagcagatg cttgagaaat ttaatttgct
    12781 tcaaagtaag attaatgata tagaaacact tttacaaaaa aaggaggaag cagaacctgc
    12841 tgaagaacca gaagaaaaaa agtttatcta taattttttc cgggtaaaag ctaaattcag
    12901 agatgatatt tttaaatttg ggattgatcc actcataata atggaagatt tatcttcatt
    12961 gggaaagatt gttgaattgc gtgttaatcg aaataatgtt ccactgttgc aagacattga
    13021 tccagaacaa tgttatatcg catgggatgt aattttaaaa accaagagca aggctgaggc
    13081 tctggatgac gtttttatat ttgtacggga tgacaacgaa atcactatta cagatgtaac
    13141 aaaaaattat gtggctgaac aggttattga agatgttcca aaattaggtg agcttttatt
    13201 atcccggggc atgttaacag agaatgaatt tgaagatgtt ttgaacgaac acgagaagac
    13261 taaaaagaag gtgggggaga tagctgttac taaaggatat attgaagaaa atgatgtatc
    13321 gttagcttta aaggaacagg atgaaataaa aaaacagata agcacctcta ctttgcgagt
    13381 agatgcaaaa aaagtagata atctcatgaa tcttttaggt gagctggtaa ttggattttc
    13441 aggtattaaa cgaattgctg aagagattga ggatgagaag gcttaccggt tgaataatgc
    13501 attgtatagc gttgaccgta ttatacggga atttcaggag catctgatgc gtgtgcgaat
    13561 ggtgccaatt ggaccgatat ttgaacaata caggcggttt atacgtgata ctgcaaaggc
    13621 gcatggcaaa tcaatacgtc tggaaataac aggtggcgat actgagattg acaagaccgt
    13681 gatagagcgg attagtgatc ctctaaaaca tttgatacgc aatgctatag atcatggaat
    13741 tgaagcacct catgataggg aagaaaaagg gaaaccgcgt gaggggcttg tctcattaaa
    13801 agcataccat caggaaggga atatgtatat tgaagtgagc gatgatggag caggtattga
    13861 taaagaaaag gtaatagaaa aagcagtggc taaaggactg ctgtcaaaaa acgatgaaat
    13921 tccagatagt acattatatg gttttctttt tgaaccgggt ttttcaacct cagaatctgt
    13981 tggtgatctt tccggcagag gcgttgggct tgatgttgtg aaaactaata ttgaggcatt
    14041 acgtggtact gttgatattc tcagtcaacc tgaaaaaggt acaacattta ttattaaatt
    14101 gccattaaca ttagctatta ttgatggtat gctggtaaga gttgcccatg aaacatttat
    14161 tattccaata ctttcaataa ttgaaagtat taaacctgtt aaagatgata ttaaaacaat
    14221 agaaagtcag ggtgaagttg ttcacatccg tggagaatat gtacctcttt tacatttaca
    14281 tcaggaattt aatcttccac aaaccataca aaatccctgg gatggattgg ttgttgttgt
    14341 tgagtctcag ggcagatcaa ttgggcttat agttgatgag ctgattggtc agca
//
LOCUS       DAOOJH010000061        14202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4082_length_14202_cov_9.997809, whole genome shotgun sequence.
ACCESSION   DAOOJH010000061 DAOOJH010000000
VERSION     DAOOJH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14202
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4082_length_14202_cov_9.997809"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLJ75_08565"
     CDS             <1..872
                     /locus_tag="PLJ75_08565"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPD05304.1"
                     /translation="YIKIAEGCSNNCSYCAIPIIRGKHRSYAPELIVDDAAQALQRGV
                     KELIVVAQDVAIYNYDGVLVNDLISRLNNLEGTFWIRLMYCHPDHISSELIEAMATLQ
                     KVVHYIDIPFQHASTKILRAMNRKGNAKRYLRLVEKLRKAIPDIAIRSTFMVGFPGED
                     DDDFAVLLDFIKEAQLDRVGVFMYSPEEGTKASVMSGMVKKATQDKRYNRLMQLQQII
                     SLQRLEKTIGNVVEVLVEEKVDANNYIGRTQYDAPDIDGIFFLTADGINVNDIVLAKI
                     TDTLEYDRIGVLI"
     gene            869..>1090
                     /locus_tag="PLJ75_08570"
     CDS             869..>1090
                     /locus_tag="PLJ75_08570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPD05305.1"
                     /translation="MNIPNLLTLSRIILIPLFLYLIFTPTIEHRIWALVIFVIASLTD
                     LLDGWTARKLKQETETGKFLDPLADKFLVI"
     assembly_gap    1091..1190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1349..2218
                     /locus_tag="PLJ75_08575"
     CDS             1349..2218
                     /locus_tag="PLJ75_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HPD05306.1"
                     /translation="MKRHYILLLLFLFFICSIFMVYAQKNVGDSSKDVNYCIQCHLTL
                     NGKPRSVVLEWENSIHAKKNLECDGCHGGNHTVNDVKQAKDKKTGFIGKPVKKDIPQF
                     CGRAECHGYALTQFKRGPHYDSVLKIGSPNCVDCHGDHNVQRSTYHIISDKTCSSCHS
                     VEYSRELVSSMISIEKNIEEIENSLEYLNQRNVESKDSMVRYIELKRYFHQLVHVFSK
                     QEIDFTKKLVEVEIEYLDKQLTNRIATIKRLDLIYIITSGFSIAIIIAFTVYVALIRK
                     RRTKVMKNFQSRS"
     gene            2237..2749
                     /locus_tag="PLJ75_08580"
     CDS             2237..2749
                     /locus_tag="PLJ75_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPD05307.1"
                     /translation="MIQDYFEKFKRWYLFNTEKIKYVGKKNDKHTCILCAIRDKAEDV
                     VNLEVHRTERFIVSINLYPFNPGHCMIFPLRHCENIHELGKEDIVALHELTIKVIDII
                     KQEFNPSGFNVGFNIGLWSGASISHIHLHIVPRFPNELGFLDVISHTRVMVVDPIVVC
                     ANLKKRFNAE"
     gene            complement(2753..2950)
                     /locus_tag="PLJ75_08585"
     CDS             complement(2753..2950)
                     /locus_tag="PLJ75_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05308.1"
                     /translation="MLPHQMRAGDFACLLCGSKLNLSISDIAVGITTGSCPMCSQPFT
                     IKLNKKDIELLLEAEETGKQQ"
     gene            3182..3925
                     /locus_tag="PLJ75_08590"
     CDS             3182..3925
                     /locus_tag="PLJ75_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05309.1"
                     /translation="MNSKRGDVMTGFRKITVTLLIVSILIAIVIIFDGEIVKKIVMLK
                     PVRVVTQGEINRMEEELSKLTKKDAKTPETINKIGVLYQNLGAKYNEKGAWDPAIDAL
                     QKALGHGRNVPVVHYQLAIAYANRGTQLGNNDDIDKAVYHYEKALQMEPNYIEAKYGL
                     AILLFYRKNERSKAIQIMNEIAKQNPKFYRARFALARFYYENKELDKSLSVYQDLYTD
                     LDKMDSTLATEYRQACKENIQRITSEMAR"
     gene            3932..5029
                     /gene="dprA"
                     /locus_tag="PLJ75_08595"
     CDS             3932..5029
                     /gene="dprA"
                     /locus_tag="PLJ75_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD05310.1"
                     /translation="MDKQNISVALSLMSSGLWQLYAQNDPGTIYYECVLPEQRTVQDY
                     YATWYSGTPLEESEKIIELCHKLNVTIISYWDDAYPQLLREIVYPPAVIYCKGILPNK
                     PYIAIVGTRNADPKSAFIARTLSGTLASAGIAVVSGLAVGIDREAHSGALKANGSTIG
                     VMANGIDMLYPASNRDIYTDIINSGTGCVLSEYPPKVRINKWTFVRRNRIVSGLSRAT
                     IIVKAAQKSGALITARYALEQNRELFVCPGHSFDEGYKGCFMLLKEGAHPVYDENDIF
                     EVLNIGSTRKEMAIMKNTNIDDTNMMESNLFAVYDNDDIVKTLRQGAMEIDSLIRLMG
                     KSSAEVLEKLTTMELQGIITRSGNIIALNNK"
     gene            5037..5645
                     /locus_tag="PLJ75_08600"
     CDS             5037..5645
                     /locus_tag="PLJ75_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05311.1"
                     /translation="MIVKKILIVMLMVLWCISFVQIFSVPPYSGDIVKIYKSGYFKEL
                     ERNMFLITDSFLQIKTMSKAEYAWIYLNDISTKYNIIINVYNSEGRLVKAPGMSQIGN
                     DTKVIPLLHELKPEAKFFITGDSYCGIIPVYRKSECNFCHQVSQQPLIGIITYSMPYD
                     GYIYYTSERIIIFVIITFAISCLLFVVIRWQPYKDIQELFDK"
     gene            5724..7826
                     /gene="topA"
                     /locus_tag="PLJ75_08605"
     CDS             5724..7826
                     /gene="topA"
                     /locus_tag="PLJ75_08605"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPD05312.1"
                     /translation="MADHKKTLVIVESPTKAKTINKYLGKDYMIASSMGHVIDLPKSR
                     IAVDVDNDFKPDYITIRGKAKLIKDLKKKALEAKNVLLATDPDREGEAISWHLSNVLK
                     DKNRDIKRIEFNEITEEAIKEAVKNARDINMDLVNAQQARRILDRLVGYYLSPILWKK
                     VKKGLSAGRVQSVALRVICEREKEIENFIPQEYWTLDAIMEHNKSEFIASLVAEDGNR
                     IDLKTKADVDRILSLIERKPFTVKKIEQKERRRKPQPPYITSKLQQDAARRLGFSASK
                     TMMIAQQLYEGVKLSGEGQVGLITYMRTDSTRVAHTAVNAVREYLQKYYSKDYISDVP
                     NIYPNKKGAQDAHEAIRPTNVFRTPESIKNDLTPDQYKLYSLIWKRFVSSQMTPEVSQ
                     VISVIFDVVNFEFRSSGSKVLFDGFTAIEKEESAQKKLLPALEVGQVLMPKSFSPEQH
                     FTTPPPRFNEASLIKFLEESGIGRPSTYAPIINTLFKRYYVVKRGKQLVPTVLGRLVN
                     DIMVSYFESLINIDFTAKMEEQLDMIEEAHNDWVSMIREFYDPFKATVSHAEEHIGDM
                     KNILDEETDLVCEKCGKKMVKRLGKFGFFLACSGFPQCTNAKPLPLGKCPKCGGDIVK
                     RSGKRRGSFYACNRYPECDFATREEVSERSCPQCGSVLFIRKIKKKGEMLVCLNEQCG
                     YSVTLLDDDKTETEVNVS"
     gene            7828..8736
                     /locus_tag="PLJ75_08610"
     CDS             7828..8736
                     /locus_tag="PLJ75_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPD05313.1"
                     /translation="MDHYIDTFIRYLISEKNASPLTVESYSKDLKQFVEAVHNYLSTD
                     DIDIKGITAEHIRQFIDICFENGNELSTIGRKIATLKSFFRYMEFHNYISINPARDIH
                     FPKQNNYLPVFLTFDQIQKLTTFQCHNFIDFRDKALLEVFYSTGARVSEIANANIVDL
                     DCEKKTLKVLGKGSKERIVFLTDDTVYWFDKYYNCRLQKFGIMTDPLFINNSGKRLTS
                     RGIFYIIEKRANMAGLWKSVSPHVLRHSFATELLNRGADIKAVQDMLGHENISTTQKY
                     THTTIKRLKELYQKCHPHAIRRDNNE"
     gene            8729..9274
                     /gene="hslV"
                     /locus_tag="PLJ75_08615"
     CDS             8729..9274
                     /gene="hslV"
                     /locus_tag="PLJ75_08615"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPD05314.1"
                     /translation="MNNVTYRGTTVLAIKKDNTLAMGCDGQVTLGDTVIKSQAVKLRK
                     VYNDKVMTGFAGSVADALTLFERFEQKLESYSGNIPRSVVELARDWRLDRALRRLEAV
                     LIVGDPQHLYLISGNGEIIEPDDGILGIGSGGNYALAAARALLRNTSLDALEIVKQSL
                     LIASEICIYSNSNIVIEEIKQ"
     gene            9274..10683
                     /gene="hslU"
                     /locus_tag="PLJ75_08620"
     CDS             9274..10683
                     /gene="hslU"
                     /locus_tag="PLJ75_08620"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPD05315.1"
                     /translation="MGDIIQFMGNNDLTPRQIVSELDKYIIGQKDAKKCVAIALRNRS
                     RRKKLDPELQEEISPKNIIMIGPTGVGKTEIARRLSKLVNAPFIKVEITKYTEVGYVG
                     RDVESMIRDLIAIAVNMVKKEYSTMVEEKALKNAYDKIVDIIIPKKGFEFDDRQVSGF
                     HMERESIFESDTMYEQMRKQYLEDLKNGNLDDMEIEYESEMPNVIPVMSVMGGSGLED
                     IDMQIQSMLGDLMPKKSKRKKSTVKDALRIFITEETEKLIDMEKVTIEAVKRVEDMGI
                     VFIDEIDKIAGAESKSGPDVSRQGVQRDLLPIIEGTTVNTKYGPIKTNHILFIAAGAF
                     NVSKPSDLIPELQGRFPIRVELSSLSEEDFRKILTVPKNSLTLQYKELLKTEGVAIEF
                     TRDGIVEIARIAQVLNDENENIGARRLHTIMEKLLEDILFDAPDLDTVHKNFTINADF
                     VKNKLEPTIKNKDLSKYIL"
     gene            complement(10689..11597)
                     /locus_tag="PLJ75_08625"
     CDS             complement(10689..11597)
                     /locus_tag="PLJ75_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HPD05316.1"
                     /translation="MNESKSIRYICKEHHLRIYATISKDLLEQLISIHHTTLHATIAL
                     GRTLNAAILLASSSLKPNSNDTLSIKIQCSGPLQEIMVQVDGRGNVRGLVSNPDVDAM
                     MQTQTLDIAKALGAGIITVTRDLGLKEPYTGVSPLLYGDIANDIAHYLTQSEQIPSAI
                     ILTVNYNNDGTLARSAGILIQTYPDTPEFVIQHIEQYLQSGFSLDRELQTTTDCKAII
                     EKIVKSNIEPLGCTPLQLNCRCNKEMLTQILVSIDNNELTAMIEKDHGAQITCAFCKK
                     QYDFTEDDLKKILLTKEKKSPNTSYQ"
     gene            complement(11590..>12204)
                     /locus_tag="PLJ75_08630"
     CDS             complement(11590..>12204)
                     /locus_tag="PLJ75_08630"
                     /inference="COORDINATES: protein motif:HMM:NF027524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05317.1"
                     /translation="TVDDTMNAIACIKNEGFNLIIQLMPGLPCDTSEKSIESAHIACK
                     LQPNGVRIYPTIVIKDTVLEKLFIEGIYTPMTLEEAVTLTAGMYSIFTMNNIPVIRMG
                     LHPFANDTSSFIVAGPYHPSFGYLVKSRYCRNQLQNMLQQATDNNSTSKRYIIELPAL
                     HSAEYLGAHRENIAFLQNMFYNSYIQCTITNTLEPILYSDSYYE"
     assembly_gap    12205..12304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12415..>14202)
                     /locus_tag="PLJ75_08635"
     CDS             complement(12415..>14202)
                     /locus_tag="PLJ75_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HPD05318.1"
                     /translation="AFLAAKVLALDDSRVTPAYRLNDNTNYYPTNKWVLFGHHFAAIA
                     GAGPLIGPVLAAQFGYLPGFLWILIGSVFAGGVHDMVILVASIRKDGKSLAEIAKEEI
                     GPVTGMAALFAVLFILIVAIAGLGLVVVNSLYNNPWGTFIIAMTIPIAIVMGIYMKSI
                     RPEKVLETSIIGIALLLITVIYGKNILGTGAEPFFTLNQHNLAVTLAIYGFIASVLPV
                     WLLLAPRDYLSSFMKIGVILLLAIGVLVVHPDLHLPALTQFIHGDGPIIPGKLFPFVF
                     ITIACGAISGFHALISSGTTPKMIEKERYALPIGYGAMLVEGFVSMMALIAAAVLIPG
                     DYLAINTHLSFETLAQMGFPVDKINELSQLVGINIAGRPGGAVSLAVGMAYIFSSIPF
                     LSHLMAYLYQFAIMFEALFILTTIDAGTRVARYVVQELGGYIYKPFGDLRSLYGNIIA
                     SLIVVFAWGYFIYTGSVQTIWRMFGTANQLLAMLALCIGTTIIIRMNKIKYIWVTLIP
                     MLFMLTTTFTAGIILIMEYIPLALSTQPDAYTYMINIVLLGLMILLALFILLDSMYKW
                     YGFITGKRSLQNNTGEPSQYSKEFPKMSD"
BASE COUNT         4592 a         2250 c         2904 g         4256 t
ORIGIN      
        1 cctatattaa aattgcagaa ggttgctcca ataattgcag ttactgtgcc attccaataa
       61 tacgcgggaa gcatcgttcg tatgcgcctg aattaattgt agacgatgct gcacaagcgt
      121 tgcagcgggg cgtaaaagag cttattgttg tagcgcagga tgtagcaata tataattacg
      181 atggtgtgct cgtcaatgac ctgattagca ggctcaataa tcttgaaggt acattctgga
      241 tacgacttat gtattgtcat cccgatcaca tttccagtga attgattgaa gcaatggcaa
      301 cactgcaaaa ggttgttcat tatattgata tcccctttca gcatgccagc acaaagatat
      361 tgcgtgccat gaacagaaaa gggaatgcta aaagatattt acgccttgta gaaaaattac
      421 gcaaagcaat ccctgatata gctatacgct caacgtttat ggttggattc cctggcgaag
      481 acgacgatga ttttgctgtc ctgcttgatt ttataaaaga agcacagctt gacagggttg
      541 gtgtatttat gtattcacct gaagaaggaa caaaagccag tgtcatgagt ggtatggtta
      601 aaaaagcaac acaagataaa cgctacaaca gactgatgca attgcagcaa ataatatcgt
      661 tgcaacgatt ggaaaaaact ataggaaatg tagttgaggt gcttgttgaa gaaaaagtgg
      721 atgcaaataa ctacatcggc agaacgcaat atgatgcccc cgatattgat ggtatttttt
      781 tcttgaccgc agatggtatt aatgtcaatg atatcgtgct ggcaaaaata actgatacat
      841 tagaatatga ccgtattgga gtactcattt gaatatacca aatttattga cattatcacg
      901 aattattctg attccgctat ttttatattt gatttttacg cccaccatag aacatagaat
      961 atgggcgctt gttatatttg tcatagcttc gttaactgat ttgttagatg gatggactgc
     1021 ccgtaaactg aagcaggaaa cggaaacagg aaaatttttg gatccgttag ctgataagtt
     1081 ccttgttatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tggatgatat
     1201 tattgcaggt gtatatgcaa atgttgttat ctcgttgata ttgtttatta tgaacatcta
     1261 tgggattgtg cttgtgtaat gtattaatgc tgtcgaaata gataatagta tgcggaggct
     1321 taatgccaaa atggtttgag gttgttttat gaaacgacat tatatactgt tattattgtt
     1381 tttatttttc atttgcagca tattcatggt atatgctcaa aaaaatgtgg gcgatagttc
     1441 taaagatgtg aattattgca ttcaatgtca ccttacgctt aatggaaaac cgcggtcagt
     1501 agtgcttgaa tgggaaaaca gcatccatgc aaaaaaaaat ttagagtgcg atggatgtca
     1561 tggcggtaac cacacagtaa atgatgtaaa acaggctaaa gataagaaaa cagggtttat
     1621 tgggaaaccg gtaaagaagg atataccgca gttttgtggt agagcagaat gccatggtta
     1681 tgctttaaca caatttaaac gcggtcctca ttatgattcg gtattaaaaa ttggttcacc
     1741 caattgtgta gattgccatg gcgatcataa tgttcaacgt tcaacatatc atattatatc
     1801 cgataagaca tgttccagtt gtcatagtgt tgaatattca cgtgagctgg ttagttcaat
     1861 gatcagtatt gagaaaaata ttgaagagat tgaaaactca ttggaatatc taaaccagcg
     1921 caatgttgaa agtaaagata gcatggtacg atatattgaa ttaaaacggt atttccatca
     1981 attagtacat gtattctcaa aacaagaaat agactttacc aaaaaattgg tagaggttga
     2041 aattgaatat ttagacaaac aattaacaaa ccgtattgca accattaaac gtcttgattt
     2101 aatctatatc ataacaagtg gatttagtat tgctataatt attgctttta cagtatatgt
     2161 agcattaata cgaaaaaggc gaacaaaagt aatgaaaaat tttcaatccc gctcatgata
     2221 tccgtggagt atttgtgtga tacaggatta ttttgaaaaa tttaagcgat ggtatctttt
     2281 taacactgaa aaaataaaat atgttgggaa aaagaatgat aagcatacct gtatcctctg
     2341 tgcaatacgg gataaagcgg aggatgttgt taatctggaa gtgcatcgta cagaaaggtt
     2401 tattgtttcg ataaatttat atccattcaa tcccggtcat tgtatgattt ttcccttgcg
     2461 gcattgtgag aatatacatg aattgggaaa agaagatatt gtggctctcc atgaattgac
     2521 aattaaggtt atcgatataa taaaacaaga gtttaatcca tcaggtttta atgttggatt
     2581 taatataggt ttgtggagtg gcgcaagcat atcgcatatt catttacata ttgtaccgcg
     2641 gtttcctaat gagctgggat tcttagatgt tatcagtcat acacgagtaa tggttgttga
     2701 tcctatagtt gtttgtgcaa atttgaaaaa acgattcaat gcagagtaat agttactgtt
     2761 gtttgccagt ttcttctgct tcaaggagca gttcaatatc ttttttattt aattttattg
     2821 taaaaggttg gctacacatt gggcaggaac cagtagttat gccaacagca atatcgctta
     2881 tggaaaggtt aagttttgat ccgcacaaca aacatgcaaa gtcgccagca cgcatctgat
     2941 gaggcagcat aaatacaact ccctggtaaa gtagtttgtt aaggttaagt ggtaaatata
     3001 aattgctata aaaatgtcaa gttttttata ctctggtgag attgtttaac aagctattga
     3061 atcaacaatt gaatcaacaa ttgaaaaaaa gtgttttaca actatcgtat gctcaccatg
     3121 atattaaata gtgaagtttt aataatgtcc cttgagtttt ataaatgtaa gatataacag
     3181 tatgaatagt aaacgaggtg atgtcatgac agggtttaga aaaattacag taacattgct
     3241 tattgtcagc attttgattg ctatagtgat aatttttgac ggagaaattg taaagaaaat
     3301 tgttatgtta aaaccagtaa gggtagtaac tcagggcgaa attaatcgca tggaagaaga
     3361 attatcaaag cttactaaaa aagatgcaaa aactcctgaa acaattaaca aaattggtgt
     3421 tttatatcaa aatttagggg caaaatataa tgaaaaaggt gcatgggacc ctgcaatcga
     3481 tgcattgcaa aaagcattgg ggcatggcag aaatgtcccg gtagtgcatt atcagttagc
     3541 tatagcatat gctaacaggg gaacgcagct gggaaataat gatgatatag ataaagctgt
     3601 gtatcactat gaaaaagcat tgcagatgga accaaattat attgaagcaa aatatggatt
     3661 ggctatactg ttattttatc gtaaaaatga acgttctaaa gccatacaga ttatgaatga
     3721 gattgctaaa caaaatccaa aattttacag agcacggttt gctttagcca ggttttatta
     3781 tgaaaacaaa gaacttgata aatcattatc tgtctatcaa gatttatata cagaccttga
     3841 taaaatggat tctacattag caacagaata cagacaggca tgtaaagaaa atattcaacg
     3901 gattacatct gaaatggcac ggtaatgttg tatggataaa caaaatattt ctgtggcact
     3961 atcgctcatg agttccgggt tatggcaatt atatgctcag aatgaccccg ggacaatata
     4021 ttatgaatgt gttttgccgg aacagagaac tgtacaggat tattatgcta cgtggtatag
     4081 tggcacgcca ctggaagaat cagaaaagat tattgaattg tgccataagc ttaatgtgac
     4141 tattatatca tactgggatg atgcatatcc acaacttttg cgagagatag tctatcctcc
     4201 tgctgttata tattgtaaag gcatattgcc caataaacca tacattgcaa tagtgggtac
     4261 caggaatgct gatcctaagt cagcctttat tgcacgtact ttatcgggga cacttgcttc
     4321 agcaggtatt gctgttgtca gtgggttggc agttggaatt gatagagaag cgcatagtgg
     4381 tgcattgaaa gcaaatggct caaccattgg tgttatggct aatggaatag atatgttgta
     4441 tcctgcaagc aacagggata tctacacgga tattattaat tcgggcactg gatgtgtatt
     4501 gtcagaatat ccaccaaagg tacggattaa caaatggacc tttgtaaggc ggaatagaat
     4561 agtcagtggt ttatctcgag ctaccatcat agttaaagca gcccagaaaa gtggagctct
     4621 tattactgcc cggtatgcat tggaacagaa tagggaactg tttgtttgcc cgggacattc
     4681 ttttgatgaa gggtataaag gttgtttcat gctattgaaa gaaggtgcac atcctgtcta
     4741 tgatgagaat gatatctttg aagttttaaa tatcggcagc accagaaaag aaatggctat
     4801 aatgaagaat accaatatag atgatactaa tatgatggag agcaatttgt ttgctgttta
     4861 tgacaatgat gatattgtaa aaacattgag gcagggggct atggaaattg attcactaat
     4921 ccgtttgatg ggtaaaagca gcgctgaagt tcttgaaaaa ttgactacta tggaactgca
     4981 gggtattatt acacgatccg ggaatataat agccttgaac aataaataaa gattgtatga
     5041 tagttaagaa aatattaata gttatgctca tggtgctctg gtgtataagt tttgtccaga
     5101 ttttttctgt tccaccgtat agtggcgata tagttaaaat atataaatca ggttatttca
     5161 aagaactcga aaggaatatg tttttgatca ccgattcttt tttacagatt aaaaccatgt
     5221 caaaagcaga atatgcatgg atttatttga atgatatatc aacaaagtat aatattatta
     5281 tcaatgttta taattctgag ggcagattgg tgaaagcacc aggaatgagc cagattggta
     5341 atgatactaa ggttatacca ttgctgcatg agcttaaacc tgaagcaaaa ttttttatta
     5401 cgggcgatag ttattgtgga ataattcctg tgtatagaaa atccgaatgc aatttttgtc
     5461 atcaggtttc acagcaacct ttaattggaa ttataaccta ctctatgccg tatgatggct
     5521 atatctatta tacatcagaa cggattatta tatttgttat tataacgttt gccataagtt
     5581 gtttgctttt tgtagtcatt cgatggcaac cgtataagga tattcaggaa ttatttgaca
     5641 aataatgagt agctattttc atgatgtgca atcaataata ccatgaacta tcgcttattt
     5701 taatgtatta aaaggaaaat aatatggctg atcataagaa aacacttgtt atagttgagt
     5761 cacctacgaa agctaaaaca ataaataaat atttaggcaa ggattatatg attgcctcgt
     5821 caatggggca tgtcattgat cttcccaaat cacgtatagc cgttgatgtg gataatgatt
     5881 ttaaacctga ttatataaca atacgtggca aggctaaatt aataaaagat cttaagaaaa
     5941 aagcccttga ggctaaaaat gttctccttg caactgaccc tgacagagaa ggcgaggcaa
     6001 tttcctggca tctatcaaat gttttaaaag ataaaaacag agatataaag cgaatagaat
     6061 ttaatgaaat tactgaagaa gcaattaaag aagcagtgaa gaatgcacgc gatattaata
     6121 tggatctggt taatgcacag caggcacggc gtattcttga tagactggtt ggttactatt
     6181 taagcccaat attatggaaa aaagtaaaaa aaggattatc ggcaggtcgt gtgcaatcgg
     6241 ttgcattgag ggtaatctgt gaacgtgaga aagaaataga aaattttatt ccacaggaat
     6301 actggacatt agatgctatc atggagcata acaaatcaga atttattgct tcacttgtag
     6361 ctgaagatgg taacaggatt gatttaaaga caaaagctga tgttgacagg atattatcct
     6421 taattgaaag gaaacctttc actgttaaaa aaattgaaca aaaggaaaga agacgaaaac
     6481 cgcagccacc ttatattacc agcaagttgc aacaggatgc tgcccgccga ttgggatttt
     6541 ctgcaagtaa aaccatgatg attgcacagc agttatatga aggtgtaaaa ttatccggtg
     6601 aggggcaggt tggtttaatt acttacatgc gtactgattc cacacgagta gctcatactg
     6661 cagtcaatgc tgttcgtgaa tatttgcaaa agtattattc taaagactat atttctgatg
     6721 tcccaaatat atatcccaat aaaaaaggag ctcaggatgc tcatgaagca atacgtccaa
     6781 ccaatgtgtt cagaacacct gagtctataa aaaatgactt aacgccagat caatataaat
     6841 tatattcact tatatggaaa cgatttgttt cttcacaaat gacccctgaa gtttcacagg
     6901 tgattagtgt tatatttgat gttgtaaatt ttgaattcag gtcttcagga agtaaggtgt
     6961 tgtttgatgg gtttactgca attgaaaaag aagaaagtgc ccagaaaaaa ctgttgcctg
     7021 ctttagaggt tggacaggtt ttaatgccca aatcgtttag ccctgaacaa catttcacaa
     7081 caccaccacc acgttttaat gaagcatcac ttataaagtt tttagaagaa tcagggattg
     7141 gtagaccatc aacctatgcg cctattatta ataccctgtt taaacggtat tatgtggtta
     7201 aacgtgggaa acaactggta ccaacagttt taggaagact ggttaacgat attatggtat
     7261 cgtattttga atcgttaatc aatattgatt ttaccgcaaa gatggaagaa caattagata
     7321 tgatagaaga ggctcataat gattgggtta gcatgatacg tgaattttat gatccattta
     7381 aggcaacagt aagtcacgcc gaagaacata taggggatat gaaaaatatc ctggacgaag
     7441 aaactgattt agtatgtgaa aagtgtggca aaaagatggt taaacgtttg ggtaaatttg
     7501 ggtttttcct tgcctgctca ggatttccgc agtgtaccaa tgcaaaacca ttgccgttgg
     7561 ggaaatgtcc aaagtgtggc ggtgatattg taaaacgttc ggggaagcgc cgtggttcat
     7621 tttatgcgtg taaccgatac cctgaatgtg attttgcaac ccgtgaagag gtatcagagc
     7681 gttcatgccc acaatgcggt agcgtattgt ttattcgcaa gataaagaaa aaaggcgaaa
     7741 tgcttgtatg tttaaatgag cagtgtggtt attcggtaac attgctggac gatgataaaa
     7801 ctgaaacaga agtaaatgta agttgatatg gatcattata ttgatacatt tatacgctac
     7861 cttatttctg agaaaaatgc atcgccatta acggttgaat catatagtaa agatttaaaa
     7921 caatttgttg aggcagttca taattattta tcaacagatg atattgatat caaaggcata
     7981 accgccgagc acattcgtca atttattgat atctgctttg aaaatggaaa tgaattgtct
     8041 actattggaa gaaaaatagc aacattgaaa tcttttttta ggtatatgga atttcataat
     8101 tatataagta ttaatcccgc acgggacatt cacttcccta aacaaaataa ctatcttccc
     8161 gtttttttaa cctttgatca gatacaaaaa cttacaacat ttcaatgtca caattttatt
     8221 gattttcgtg ataaggcatt gcttgaggta ttttactcaa ctggcgcacg tgtttccgag
     8281 attgctaatg caaacattgt tgaccttgat tgtgaaaaaa aaacattgaa ggtgttgggt
     8341 aaaggttcaa aagaaagaat tgtttttttg actgatgata cagtttactg gtttgataaa
     8401 tattacaact gtcgcttaca aaaatttggt attatgaccg atccattatt tattaataat
     8461 agtggtaaac ggttgacatc gcgtggtatc ttttatatta ttgaaaaaag ggcaaacatg
     8521 gcaggtttgt ggaagtcggt tagccctcat gttttgcgtc atagctttgc cactgaactg
     8581 cttaacagag gtgcggatat taaagctgtg caggacatgt taggacatga gaatatttca
     8641 acaacgcaga aatatactca tactaccatt aagcgattaa aagaattata tcaaaaatgt
     8701 catccccacg caatcaggag agacaacaat gaataatgtt acataccggg gaaccactgt
     8761 tcttgctata aagaaagaca atacccttgc catgggatgt gatggccagg ttacattggg
     8821 tgatactgtt ataaaaagtc aggcggttaa attacgcaaa gtgtataatg ataaagtaat
     8881 gaccggtttt gccggttcgg ttgctgatgc actaaccctt tttgaacgct ttgaacaaaa
     8941 gctggagtcg tattccggaa atattccgcg ttcggttgtt gagctggcgc gtgactggcg
     9001 gctggacaga gccctgcgca ggctggaggc tgtactgatt gttggcgatc ctcagcatct
     9061 ttatcttata agcggaaatg gtgagattat agagccggat gatggcattt taggcattgg
     9121 ttcgggtggt aattatgcat tggcagcagc cagggcactg ctcagaaata catcattaga
     9181 tgctcttgag atagtgaaac aatcgttatt gattgcctcg gaaatttgca tttattccaa
     9241 tagcaatatt gttattgagg agataaaaca ataatgggtg atataataca gtttatggga
     9301 aacaacgatt taactcctcg tcaaattgta agtgaactgg ataaatatat tattgggcag
     9361 aaggatgcta aaaaatgtgt ggcgatagct ctcagaaaca gaagccgtcg taaaaagtta
     9421 gatcccgaat tacaggaaga aatatcgcct aaaaatatca tcatgatagg acctaccggt
     9481 gttggcaaaa ccgaaattgc ccggcgacta tcgaaacttg ttaatgctcc ttttataaaa
     9541 gttgaaatta ctaaatatac tgaggttgga tatgtaggtc gtgatgttga atcaatgata
     9601 cgagatttaa tagcaattgc tgtcaatatg gtaaaaaaag aatatagcac aatggttgag
     9661 gaaaaggcgt tgaagaatgc ctatgataag atagttgata taattatccc taaaaaaggt
     9721 tttgaatttg atgacaggca ggtttctgga tttcatatgg agcgcgaatc tatttttgag
     9781 tctgatacca tgtatgagca gatgaggaaa caataccttg aagatttaaa aaatggaaat
     9841 cttgatgata tggaaattga atatgagtct gaaatgccaa atgttatccc ggttatgtcg
     9901 gttatgggtg gttccggact tgaggatatt gatatgcaga tacaatccat gctgggcgat
     9961 cttatgccaa aaaaatcaaa acggaaaaaa agtactgtta aagatgcact caggattttc
    10021 ataaccgaag aaacagaaaa gcttatagac atggaaaagg tcactattga agctgttaaa
    10081 cgtgtagagg atatgggtat agtttttatt gatgaaattg ataaaattgc tggagcagaa
    10141 tcaaaatcag ggcctgatgt ttcacggcag ggggtacagc gtgatttatt acccattatt
    10201 gaaggtacaa cggtaaatac caagtatggt cctataaaaa caaatcatat actatttatt
    10261 gctgctggtg cattcaatgt gtcaaagcct tctgatctta tacctgaact tcagggaagg
    10321 ttccccatca gggtggaatt atcttcttta tccgaagaag attttagaaa aatcctcaca
    10381 gtacctaaaa actcattaac gcttcaatat aaggaattat taaaaacaga aggagtggct
    10441 attgagttta ccagagatgg tatagtagaa attgctcgca tagcacaggt attaaatgat
    10501 gaaaatgaaa atattggagc acgccgattg catacaataa tggaaaaatt gcttgaggat
    10561 attttgtttg atgcgcctga tcttgatacg gtacataaaa actttactat caatgctgat
    10621 tttgtaaaga ataaattaga acctactata aagaataaag atttatcaaa atatatactg
    10681 taactttttt attgatagct ggtattgggt gatttttttt cttttgttaa aaggattttt
    10741 tttaaatcat cttctgtaaa gtcgtattgt tttttacaaa atgcacatgt tatttgtgca
    10801 ccatggtctt tttctatcat cgctgtaagt tcattattgt caatagatac aagtatctgg
    10861 gtaagcattt ccttattaca cctgcagttg agctgcaatg gtgtacatcc taatggttct
    10921 atgttacttt taacgatttt ctcaattatt gccttacaat cagtagttgt ttgcaattcc
    10981 ctgtctaatg aaaaaccact ttgcagatat tgttctatgt gctgaataac aaattcgggt
    11041 gtatcgggat atgtttggat cagtatccca gcagagcgtg ctaacgttcc atcattgtta
    11101 taattaacag taagaatgat ggccgaaggt atttgttcag attgggttag gtagtgggca
    11161 atatcgtttg caatatcacc ataaagaaga ggtgatacac ccgtatatgg ttcttttaat
    11221 cctaaatcac gagttacagt aattatacct gcacccaatg cttttgctat atcaagtgtt
    11281 tgtgtttgca tcatggcatc aacatctgga ttgcttacca atccacgcac attacccctc
    11341 ccatctacct gtaccataat ctcttgcaat ggcccgctgc actgaatttt aatgcttaat
    11401 gtgtcatttg agtttggctt taatgatgaa gaagccagta agatcgcagc atttaaagtt
    11461 ctccccagtg cgatggttgc atgaagggtt gtatgatgta tggagattaa ttgttccagc
    11521 aaatcttttg agattgttgc atatattctg agatggtgct ctttacaaat gtatcgtatg
    11581 cttttgcttt cattcatagt aactatcgct atatagtatt ggttccaggg tatttgttat
    11641 ggtgcattgt atataactat tataaaacat gttttgtaaa aatgcaatgt tttctctatg
    11701 agctcccaga tattctgctg aatgcaaagc tggcaattca atgatatagc gttttgaggt
    11761 actattgtta tccgtagcct gctgcaacat gttttgtagc tgattgcggc agtatcgtga
    11821 tttaacaaga tagccaaacg atgggtgata tggtcctgca actatgaagc tgctggtgtc
    11881 atttgcaaag ggatgtaatc ccatccttat gaccggaatg ttattcatgg tgaagattga
    11941 atacatcccg gcagtaagtg ttactgcttc ttccagtgtc attggcgtat agattccctc
    12001 aatgaaaagc ttttctaaca ctgtgtcttt aataacgatg gtagggtaaa tccttacacc
    12061 gttgggctgt agcttgcagg ctatatgagc cgattcaatg gacttctctg acgtatcaca
    12121 tggcaaaccg ggcataagct gaataataag attaaatcct tcgtttttga tacaggcaat
    12181 cgcgttcatg gtatcatcaa cagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12301 nnnngtatta ctggtataca cctggttgca gaacacacag cggtgcctgc aaccaaggtg
    12361 aggtatgaag aatggtattg ttacatttgt ttttggcatt caactcctta ctgtttaatc
    12421 gctcattttg ggaaattctt ttgaatattg tgacggttca ccggtattgt tttgtaatga
    12481 ccgtttgcct gtgataaaac cataccactt atacattgaa tcaaggagta taaataacgc
    12541 taacagtatc ataagtccca gcaacacaat gtttatcatg taggtgtatg catccggttg
    12601 tgttgacaat gcaagcggta tgtactccat aataagaatt atccctgcag taaaggtggt
    12661 agttaacata aaaagcatgg ggatgagcgt aacccagata tattttattt tgttcatcct
    12721 gatgatgatg gttgttccaa tacacaatgc cagcattgcc agcaattgat tggctgttcc
    12781 aaacatgcgc catattgttt gcaccgaacc ggtataaata aaataccccc acgcaaaaac
    12841 aacaataagg cttgcgataa tattgccgta taatgatctg aggtcgccaa aaggtttgta
    12901 tatatagcca cctaattcct gtactacata ccgtgctaca cgtgtaccag catcaattgt
    12961 tgtcagtatg aacagtgcct caaacatgat ggcaaattga tacaggtatg ccatgagatg
    13021 acttaagaaa ggaatggacg aaaagatata tgccatccca accgcaagtg ataccgcacc
    13081 accaggcctg ccagctatgt ttattccaac cagttgtgat agctcattaa ttttatctac
    13141 cggaaaaccc atctgtgcca gggtttcaaa ggaaaggtga gtattaattg caagatagtc
    13201 accggggata agtacagccg cagcaatgag tgccatcatg gaaacaaatc cctcaacaag
    13261 cattgcacca tatcctattg gcagtgcata ccgttctttc tcgatcatct ttggtgtggt
    13321 gcctgatgag atgagcgcat gaaatcctga aatagcacca caggcaatgg tgatgaacac
    13381 aaacgggaaa agcttaccgg gtataattgg cccatcgcca tgtataaatt gtgttaatgc
    13441 cggtagatgt aaatcagggt gcactactaa aacgccaatt gctaaaagca ggatgacgcc
    13501 aattttcata aatgatgata gatagtcacg aggggcaagc aacaaccata ccggcaaaac
    13561 acttgcgata aaaccatata tagcaagtgt aacggcaagg ttatgctgat ttaatgtaaa
    13621 aaaaggttca gcacctgttc ctaaaatgtt tttgccatag ataacagtga taagtaataa
    13681 agcaattcct atgatagaag tttccaatac tttttcgggg cgaattgact tcatataaat
    13741 acccatcaca attgctattg gtatggtcat cgcaatgata aaagttcccc atgggttatt
    13801 gtataatgaa tttacaacga caagccccaa tccggcgatg gccactatta aaataaacaa
    13861 tactgcaaag agcgctgcca ttcctgttac aggacctatt tcttctttgg ctatctcggc
    13921 aagcgacttt ccatccttac gaatagaagc aaccagtata accatatcat gaacaccgcc
    13981 ggcaaagacc gaacctatta aaatccataa aaagcctgga agatacccaa actgagcggc
    14041 taacacaggt ccaattaaag gacctgcacc ggcaatggct gcaaagtgat gcccaaataa
    14101 tacccattta ttggtaggat agtaattggt gttatcattc agccggtaag caggtgtgac
    14161 gcgggaatca tctaatgcaa gtaccttcgc agctaaaaat gc
//
LOCUS       DAOOJH010000062        13581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4267_length_13581_cov_11.457489, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000062 DAOOJH010000000
VERSION     DAOOJH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13581
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_13581_cov_11.457489"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..1441
                     /gene="gmd"
                     /locus_tag="PLJ75_08640"
     CDS             266..1441
                     /gene="gmd"
                     /locus_tag="PLJ75_08640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD05319.1"
                     /translation="MKRALITGVTGQDGSYLTELLLSKGYEVHGIKRRSSLFNTERID
                     HVYNDPHEGGSFFLHYGDLTDSLNVTEIIQKVKPDEIYNLAAQSHVAVSFVQPEYTAN
                     VDAMGTLRILESVRLLGLQDKVKIYQASTSELFGKAVEFPQSENTPFYPRSPYGVAKL
                     FAYWITINYREAYGMFACNGILFNHESSRRGETFVTRKITRGVARILFGLDKKIYLGN
                     LNAKRDFGHAQDYVKGMYLILQQPKPDDYVLATGITTEIREFIALAFDEVGLTVTFQG
                     EGIKECGIVISCHEDVLLSRLQSLHCAPEHKENILQTARACLNKVVVGIDQRYFRPSE
                     VDILIGDPTKAREKLGWEPRHTVKDIVSDMIIGDLEKNYREYVLKTSGFTMSRSCGV"
     gene            1528..1917
                     /locus_tag="PLJ75_08645"
     CDS             1528..1917
                     /locus_tag="PLJ75_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05320.1"
                     /translation="MQKVDILHRYLSEHSVPELIEKKLDANSFLTNNFAYHALRIGNS
                     IGDNLDISIEIIILDEIAKKYNLILNTTEHAELHTQGIAESDLDNLVQAAILFENIKN
                     NKKLYKEILRKISYFIRKEFYPIIHQE"
     gene            complement(1918..>3227)
                     /locus_tag="PLJ75_08650"
     CDS             complement(1918..>3227)
                     /locus_tag="PLJ75_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD05321.1"
                     /translation="ISEHALIKANDKLCQTIAHTISSSESFLTAEKRALKRSMILQDI
                     VKGLSKTSIEGFVYAAVYDLNGILAEKKYTYAAHTNAKLHGKYFSKDLVKQFESLNTT
                     VKERIELSLDSGQTWCYRYRIPFEFFKSKVGIIEIVFTEDAILGPVHKARLLMLIIAA
                     ILLSASIYAAMRIARQMVQPLVQLTTGVIKVKDGDLTTRIDVTTHDEIGILTEEFNTM
                     IDHLREKLKMQKFVSESTISMIHEKRDDELDLGGTNKNMAFLFSDIRGFTAMSEKLPP
                     EQVVSILNEYLDLQAAIIKKHGGDIDKFVGDEIMAVFEGDNKAESAVEAAVDIIESIQ
                     KLNKQREKEGAVTITVGIGIHMGNVVHGRMGSKDRMDFTSIGDAVNLSARLCSHADAQ
                     TILVSEAIIINLSKKKFKGKKLGPIKVKGKEQPIPIFQITGKA"
     assembly_gap    3228..3327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3328..3539)
                     /locus_tag="PLJ75_08655"
     CDS             complement(<3328..3539)
                     /locus_tag="PLJ75_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05322.1"
                     /translation="MNNSTTNPIYSFIQSIPFLTNIIKKGGIRLKWVTAVSALMVIVI
                     SIISFLLITISEHALIKANDKLCQTIA"
     gene            3941..4789
                     /locus_tag="PLJ75_08660"
     CDS             3941..4789
                     /locus_tag="PLJ75_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HPD05323.1"
                     /translation="MIINHNISAIFAHRTLKFHDWAITKDMEKLSSGMRINKAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQEVHDILQRVRVLAVQSANGIY
                     TPEDRQQIQVEVSELVDEIDRIASQAEFNKMKLLTGAFARLHPSASMWFHMGPNMHQR
                     ERVFIETMTTAALGLRNAVTLTFISISTPGKANAVIGLADEALRQISKQRADLGAYYN
                     RLEHAAKGLMNAYENIQASESRIRDTDMAEQMAEFTKNQILTQAATAMLAQANTKSQT
                     VLQLLK"
     gene            complement(4909..5880)
                     /locus_tag="PLJ75_08665"
     CDS             complement(4909..5880)
                     /locus_tag="PLJ75_08665"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPD05324.1"
                     /translation="MATYQHSTGTFIGKGGIEIFFQQWEVANPKAVLIIIHGLGEHSG
                     RYGNLIDALQNKGIAIFGLDHRGFGRSGGKRGHTDSFMDYIYDMKIFVNMIKDKNPNK
                     PVIMLGHSMGGVLAIKYALTHAEDLDALILSSPGLVPTIKVPAWKEKLAIFLSKRIPS
                     FTMPSGLDASTISRDKEVVKKYMEDPLVHDKVTPRFYVEMMKTIEECINRCGEIKMPL
                     LLFQGTADALVKDEASKIVYQKASSRDKTLKLFEGLYHETMNELEADRKKVLKVVTDW
                     ILAHSKKTSGVKHAAAPKTKTKAAAKKPKAPQKKVSKSNDKSKSKKK"
     gene            6212..6580
                     /locus_tag="PLJ75_08670"
     CDS             6212..6580
                     /locus_tag="PLJ75_08670"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPD05325.1"
                     /translation="MEFQKLRVAFVLLLCTIGFGTTGYYIFENMKPFDAFYMTMITIS
                     TVGFHEVKPLTNIGRIITITIILSGVSISAYSIGKLLRLLIEGELRKEFWRKKLERTI
                     QSLSNHFIICGFGRIGKLIC"
     gene            6611..6871
                     /locus_tag="PLJ75_08675"
                     /pseudo
     CDS             6611..6871
                     /locus_tag="PLJ75_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
     gene            6983..7201
                     /locus_tag="PLJ75_08680"
     CDS             6983..7201
                     /locus_tag="PLJ75_08680"
                     /inference="COORDINATES: protein motif:HMM:NF014171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HPD05326.1"
                     /translation="MEELKVGHNSSMVRKTLVESNLRRDYGVIIVAIKKINGQMIFNP
                     QSSEILDAGDVIVALGKKYLMEQMSKVL"
     gene            complement(7249..9102)
                     /locus_tag="PLJ75_08685"
     CDS             complement(7249..9102)
                     /locus_tag="PLJ75_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD05327.1"
                     /translation="MSSEDSGATHINTTTLQTASNMVLLFQLPACVISISGEVIALNT
                     PFSDLINMNQLCLDITHPFYPEYRKQIATSYLKALKGISRQCFAVFRNKDNQRISAEL
                     YIFPVFQGKTIVALFVILQIVDGRILSFDKSTETLINDFDKNYESQLYEYSPFPILQI
                     DQSGNIIKASSSIQELIGYEVDDLKQNRNLLYRAMNHYDFNRMRITLSQIFNGTIAYK
                     RIGEIRFITKEKDEKWVNASCYLNHSPDFQPVVEIILEDITRIKRLEYQIQQLSRFHI
                     IGDLTKGFLHSFNNIINVLLNKSQWLLQITEKPPVHEGLKLIIKTAEEGIQQVRRIEE
                     FIGRDKLEEKTNINIVEAVEDAIEFAKIQFKVDEASKRRYISIERRYFSLLSMYGDPH
                     LFRDLLLSTIFKVASVITSKGTIEIILKKNSTLSITMRTKKDTSEVKDDSIFISSIDL
                     LKLAEQNHAKILEEESLYDYSISIILPEENISPIPQNEQQVPQAKLRDKDICIVEDEP
                     ALQEIMYEVFDSLGNRVSVFNNADQALESFKINNFDIVIADYGLPGITGLDLLTKIKE
                     QKEETLTVLLTGWLLSNIKAYKNVIDIYMHKPFKLDILIHEISNELYKRNK"
     gene            complement(9178..>9482)
                     /locus_tag="PLJ75_08690"
     CDS             complement(9178..>9482)
                     /locus_tag="PLJ75_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPD05328.1"
                     /translation="KDVTQESFAREVLQWRGKVVVNFWAPWCRLCMRQNAVLKNLSQS
                     SAQAKFVTINADENQQIIKNYHIDAIPTILLFENGKIIEKMAGFQSPDTLQAKLSQ"
     assembly_gap    9483..9582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9583..10904)
                     /locus_tag="PLJ75_08695"
     CDS             complement(<9583..10904)
                     /locus_tag="PLJ75_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05329.1"
                     /translation="MRQKLKSFCILFLLVSVVTCASYDYNKQIGEIEKLFYSGQYKEA
                     ANKLLPAINTKSKDQLLYMMECGLILQVAGDYENSNKVFIEAAKIADQITISVSKQAA
                     SLLLNETTTDYNGEDFERVLIHMYLGINYLMLSKPDEARVEFKKVNDLLRDISVTTGK
                     SYKQNIMAKYLTAIAFEQIADQDNDMNDREFAYIEYKQIYELNPALDIVYRDLQRLAK
                     QLGDTDDYKMWIGKFGKRDTIPKDAGELVVIVQNGKGAIKKSRGSLLSDQAMKSSIQV
                     TLNGMSLQEGVTIAGVMIALKNAENPIPKFVKREGKINYLVVNVNGKDIERSHMLEDI
                     ATTAVKTLEDDYGRIYAKVAAGIAVKAAASVAAGYAAKKLAEQSDSTKNFAGLIGMVA
                     GAGVGVGLLSQIKPDLRCWHTLPENFQLSRLFLPPGTYTITFKYVDNAG"
     gene            complement(10920..11492)
                     /locus_tag="PLJ75_08700"
     CDS             complement(10920..11492)
                     /locus_tag="PLJ75_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05330.1"
                     /translation="MKKLSLLLIIFIIGFIACGGGVQYKDASKAEGSREWGPKEIKMT
                     VNTMVESMYSFLKDEWKKPAYIEVKQIRNKTSEHIDTKMLADEITTNLIKRRIKFVDA
                     SLTTDAISEMEKGMTGMVDPESAIPVGELKSPNMYLTGEVRENVRTVGSKTVQYLVVT
                     LKLINLKTQVVEWQDQQEFLKSASKKKITF"
     gene            complement(11610..12632)
                     /gene="mnmH"
                     /locus_tag="PLJ75_08705"
     CDS             complement(11610..12632)
                     /gene="mnmH"
                     /locus_tag="PLJ75_08705"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPD05331.1"
                     /translation="MQIPSITFFDSCSITDKVYVDVRAPVEYTNDHIPDAVNYPLLDD
                     EQREYIGTLYHTRGRDEALMEGIEIVSPKLKPMVSFFSLLSQSSSTIIVYCFRGGMRS
                     ESVVSFLNSIGFNLFKLEGGYKAYRQFIRNYLTNYYFTIPVVTVYGLAGSGKTKILHH
                     NPYSLDLEGCAGNRSSLFGSLGMPDHSQKYFESRIVHILQTINVWPYCLLEGESRKIG
                     NCHIPPIILEAMQKGIALWLDTPIDVRIQHIYQEYMPYITEEAIIAIVDKLQPALGKT
                     KTQYCKQLFCHGKYLEFIQFLLQHHYDPEYQHSIKNISFTASINYTEPHDTANTIDAL
                     IHSMLQ"
     gene            complement(12647..13306)
                     /locus_tag="PLJ75_08710"
     CDS             complement(12647..13306)
                     /locus_tag="PLJ75_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05332.1"
                     /translation="MTLQPEEMLEEWDWEKGIPTGKSIPRSIAHQCGIAHEGVHLWIV
                     RSIPIPQLVFQKRASHKDMYPGYLDITVGGHVPYGLDTNKIHKEAYEEIGIIPDDNKL
                     IDLGYFRYEENNATLFHREFQRVYLYMSDEPLTAYSFNDNEVNAVYAIPVDYCKAIFT
                     DDYPMEVEAYNGYQLLTTFVSKNNFHPLLFAPSMSHYMDVLFTAIEQLLQFGTVTIRM
                     S"
BASE COUNT         4074 a         2311 c         2457 g         4539 t
ORIGIN      
        1 tattacatga tatataatcg attaaaaatt gcaaataaaa aataaagtat atgatctgtg
       61 tatagaagaa tatataccac gccttctttg ctttgctcag ggaatggtga aaatttactt
      121 taaacagcta tttttttctt gtatattata gacgcttcag ggagattaac attataactt
      181 ttttataagt aagaagatga tggcactatg atttcaagta aggttcgttt ttaatgatta
      241 tagaacagtg gatcatgagg gtacaatgaa aagggcactt attacggggg taacggggca
      301 ggatggctct tacctgacgg agttgttgtt atcaaagggc tatgaggtcc atggtatcaa
      361 acgtcgatca tcacttttca atacagaaag gatagatcat gtatataatg atcctcatga
      421 agggggtagt ttttttttac attatggtga tttaaccgat agtttaaacg ttactgagat
      481 aattcaaaag gtaaaacctg atgagattta taatttagct gcacaatctc acgttgctgt
      541 gagttttgtt caacctgagt acacagcaaa tgtagatgca atgggaacac tgagaatttt
      601 agaatcagta cgtttgttgg gtttacagga taaggttaaa atatatcagg catccacgtc
      661 tgagctattt ggcaaagctg ttgaatttcc tcagagtgaa aacacaccat tttatccacg
      721 aagtccttat ggtgttgcaa agctttttgc ttattggata accataaact acagggaagc
      781 atatggaatg tttgcctgta atggtatttt atttaatcat gaaagttcca gaagagggga
      841 aacgtttgtt acgcgaaaaa ttacacgagg ggttgctcgc atattatttg gactggataa
      901 aaagatttat ttaggtaatc taaatgcaaa acgagatttt ggacatgcac aggattatgt
      961 caaaggaatg tatttaatat tgcaacagcc caaacctgat gattatgtat tagctaccgg
     1021 tataacaact gagataaggg aatttattgc attggcgttt gatgaagtgg gattaactgt
     1081 tacatttcaa ggcgaaggga taaaagagtg tggtattgtt atttcttgcc atgaagatgt
     1141 attgttatca cgattacaat cgttacattg tgcgcctgag cataaagaaa acatactgca
     1201 aactgcaagg gcttgcctca ataaggttgt tgttggcatt gaccagcggt acttcagacc
     1261 ttctgaagtg gatatactca tcggtgatcc aacaaaggcg cgtgaaaaat tggggtggga
     1321 accccggcat actgttaaag atattgtttc agatatgata ataggtgatc ttgaaaagaa
     1381 ctatcgtgaa tatgtgctta aaacaagtgg ttttactatg tccagaagtt gtggggtttg
     1441 attattttgt tgtattttta catcgcttct ggcataatga tttatagatt gagaggatat
     1501 aaacaactat catagtgagg ggggagtatg cagaaagttg atatattaca taggtattta
     1561 tctgagcata gtgtgcctga gctgattgaa aaaaaattgg atgctaattc ttttttaaca
     1621 aacaatttcg cctatcatgc tttgcgtatt gggaattcaa ttggtgacaa tcttgatatt
     1681 tccattgaga tcataatact ggatgagata gctaagaaat ataatttgat attaaatact
     1741 acagagcatg cagaacttca tacccagggt attgctgaat cggatcttga taatcttgtt
     1801 caagcagcaa tattgtttga aaacattaaa aataataaaa aactatacaa agagatattg
     1861 cgtaagataa gctattttat acggaaagag ttttatccaa ttattcatca agaataatta
     1921 tgctttcccg gtgatctgga agatgggtat tggttgttct ttacctttaa ccttaatggg
     1981 accaagcttc ttccctttaa atttcttctt tgaaagattt attataattg cttcagatac
     2041 aagtattgtc tgagcgtcgg catgtgagca cagccgtgcg gatagattaa cggcatcacc
     2101 aattgatgta aaatccatcc tgtcctttga acccattctg ccatgaacaa cattccccat
     2161 atggatgcct atcccaacag taattgttac cgctccttct ttttcacgct gtttgtttaa
     2221 tttttgaatt gattctatga tatcaactgc tgcttcaact gcgctttcgg ctttattgtc
     2281 accttcaaaa acagccataa tttcatcacc tacaaattta tcaatatcac caccatgctt
     2341 ttttataatt gcagcttgca ggtcaaggta ttcgttcaga atagaaacaa cctgttctgg
     2401 aggaagcttc tcggacatgg ccgtaaaccc acgaatatca gaaaaaagga aagccatatt
     2461 cttatttgtg ccacctaaat ccaattcatc gtctcttttt tcgtgtatca tggagatagt
     2521 tgattcagaa acaaactttt gcatttttaa cttttcacgc agatgatcaa tcattgtatt
     2581 aaattcttca gtaagaatcc caatttcatc atgtgtcgtt acatcaatcc gggtagttaa
     2641 atcaccatcc tttactttta taactcctgt agttaattgc actaatggtt gtaccatctg
     2701 ccgtgcaatc cgcatagctg catatatgct ggctgataaa aggattgctg caatgatgag
     2761 cataagaagc cttgctttgt gaactggtcc taatattgca tcctcggtaa aaactatctc
     2821 tataatccct acctttgatt taaaaaactc aaaagggata cgatagcgat aacaccatgt
     2881 ttgacctgaa tctaatgata gctctatcct ttctttaacg gtggtgttta atgattcaaa
     2941 ttgtttgaca aggtcttttg agaaatattt tccatggagt tttgcattgg tatgtgcagc
     3001 ataggtatat tttttttcag caagtatccc atttaaatca tagacagcag cataaacaaa
     3061 accttctatg ctggtttttg acagtccttt tactatatcc tgcagaatca tggagcgctt
     3121 caatgctctt ttttcagcgg ttaaaaaact ttccgaagat gagatggtat gcgctattgt
     3181 ctgacataat ttatcattgg ctttgataag tgcgtgctca gaaattgnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnngct attgtctgac ataatttatc attggctttg
     3361 ataagtgcgt gctcagaaat tgtaattaat aaaaagctaa taatgcttat gacaatgacc
     3421 atgagtgctg atactgcagt gacccatttt aacctgattc cacctttttt gataatattg
     3481 gttaagaatg gtatagattg tataaatgag tatatgggat tggttgtgga attgttcata
     3541 gtagttcccc tctttgttgt tcgatagtag ctcacttatg gtattactgt atacaaatcc
     3601 aattgattgg caacattata tacgatagtt ataaataatt acacataata cctttcatag
     3661 tatatattca agaaaaagaa ttttaattaa taaaaatttt ttgttggtga tagtgtggtg
     3721 taaaattttc catgccgtaa agcttaagaa atgtgttcaa taacaaaaaa taataaaaaa
     3781 catatgaaat atcaacatat tttatgataa acgtaaataa atgccattaa gttaagttaa
     3841 ccagcctcac cagttttctg aaataagacg aatcttggaa tgaactattt atatagttaa
     3901 tgctatcacg gatgatagta tattgttcaa ggaggataca atgattataa accacaacat
     3961 cagtgcaatt tttgcccacc gaacgttgaa attccacgat tgggcaataa ccaaggacat
     4021 ggaaaagctt tcttcaggga tgcgcatcaa taaagccggt gatgatgcat cagggttagc
     4081 tgtgtccgaa aaaatgcgtt cacagattca gggtttacgg caggctgaac gtaacacaga
     4141 agatggtatg tcgatgattc agacagcaga agggtatttg caggaggttc atgacatact
     4201 acagcgtgta agagtgttag cggtacagtc agcaaacggt atttacacac cggaagacag
     4261 acagcagatt caggttgaag tttcagaatt ggttgatgaa atcgatcgta ttgcttctca
     4321 agctgaattt aataagatga aacttttaac tggtgcattt gcacggcttc atccttcagc
     4381 atccatgtgg tttcatatgg gacctaatat gcatcagagg gaaagggtat ttattgaaac
     4441 aatgacaact gctgcattgg gtttgcgaaa tgctgttacc ctaacattta tatcgatttc
     4501 aacacctggt aaagctaatg ctgttattgg attagccgat gaggcactgc ggcagatttc
     4561 aaagcaacga gctgatcttg gtgcttatta caaccggttg gaacatgctg ctaagggctt
     4621 gatgaatgca tatgagaata tccaggcatc agaaagccgc atacgagata ctgatatggc
     4681 cgagcagatg gcagaattta caaagaacca gattcttact caggcagcta ctgcaatgct
     4741 ggcgcaggca aatacaaagt ctcaaacagt attacagctt ttaaaataaa taaatctgag
     4801 atagcatcgt aacaccacta taaataaagc cgggcactcc cacccggctt tattcgtatt
     4861 attggttaac attagtaagt attgttcttt taactttgtt tagcatgttt atttcttttt
     4921 ggattttgat ttgtcattac ttttggatac ttttttttga ggtgcttttg gtttctttgc
     4981 tgcagctttt gttttagttt ttggcgctgc tgcatgcttt actccagagg ttttttttga
     5041 gtgcgctaaa atccaatcag ttacaacctt tagtactttt ttacggtctg cctccagttc
     5101 attcatagtt tcatgatata atccttcaaa aagcttcaat gttttatccc tggagctggc
     5161 tttttggtaa actattttac ttgcctcatc tttaacaagc gcatcagcag ttccttgaaa
     5221 tagtaaaagt ggcattttta tttcgccgca tctatttata cattcttcaa tggttttcat
     5281 catctcaaca taaaaacgag gagttacttt atcatgaaca agcggatcct ccatatattt
     5341 tttaaccacc tccttgtcac gggagatagt tgaagcgtcc aacccggatg gcatggtaaa
     5401 tgagggaatc ctttttgaca aaaatattgc cagtttttct ttccatgccg gtactttaat
     5461 tgttggaacc aatccagggg atgataaaat tagtgcatcc agatcctcag catgtgtcag
     5521 cgcgtattta atagcaagaa caccacccat tgaatggcct aacataataa cgggtttgtt
     5581 aggatttttg tcttttatca tatttacaaa aattttcata tcgtatatat aatccataaa
     5641 actatcagta tgtcctcgtt tacccccact gcggccaaag cctctgtggt ctaaaccaaa
     5701 gattgcaatc cctttattct gaagggcatc aataaggtta ccatatcggc cgctatgctc
     5761 tccgagccca tgaataatga ttaaaactgc tttagggtta gcaacttccc attgctgaaa
     5821 aaagatttca atacctcctt taccaataaa tgtaccggta ctatgttgat atgttgccat
     5881 aatctcacct cactttttta aaagttttaa ttactacatg tacattatta tataggtact
     5941 acaaagaact gctttccatc ttatgaataa tttaaaaaag cagcttttac ctttagtgtg
     6001 cgttaagaca gataatgaaa atatagctcc caaacccctt atataatgta tatagtaata
     6061 aatattaaag aagaataata atttttcatt atcttaaatt tatataaaaa aaatcaaata
     6121 aaatttttaa ttttaaaaat tatttacatt ctataatacg atattgatat atgattgcat
     6181 tgataatgaa tttattgtat agaagggtat tatggagttt cagaaattac gtgttgcatt
     6241 tgttctcctt ctatgtacta ttggttttgg aacaactggt tattatattt ttgaaaacat
     6301 gaaaccattt gatgcttttt atatgacaat gataactatt agcactgtag gtttccacga
     6361 agtcaaaccg ttaactaata ttgggagaat tattactatt acaatcatat taagtggtgt
     6421 atcaataagt gcatactcaa ttggaaaact tttacgcttg ttaattgaag gtgagttacg
     6481 caaagaattt tggaggaaaa aattggaacg taccattcaa tcgttatcaa accattttat
     6541 aatatgtggt tttggcagaa ttgggaaatt aatttgttag gaattatttg aaagaagagt
     6601 tccatttgta gtcattgaaa acaattctgt gactatagat gaattagaca agttaggata
     6661 cctgtatatt gatatggatg ctaccaacga tgaggctttt atcaaagctg gtattatgaa
     6721 atcaaaaggg atagtcacat cggtgatgtc tgatgcagat aatgtttata ttgtattaac
     6781 agccagaggg ttacgtggtg acatttgtat tttatcaaga ggttcagatg aaaagtcaga
     6841 acaaaaactg ttacgtgcag gagcaaacag ggtggtactt ccttatcaaa tagggggtaa
     6901 gcgtatggcc caggttttaa cacgtccaac tgttgttgat tttattgata tggcaatgat
     6961 ggattcagaa ttaggattgg caatggaaga gttaaaagtt ggtcataact catcaatggt
     7021 taggaaaact cttgttgaaa gtaatttgcg acgagattat ggtgttatta ttgtggcaat
     7081 taaaaaaata aatggtcaaa tgatatttaa tccgcaatcg tcagaaattc ttgatgcagg
     7141 tgatgttata gtagctttag gtaagaaata tttgatggag caaatgagta aagtattata
     7201 atagctataa aaaacttttt ggggtacatt attacaggct aatcattgtt atttatttcg
     7261 tttgtataat tcatttgaaa tttcatgaat caaaatatcc agcttaaaag gtttatgcat
     7321 atagatatcg attacatttt tatacgcctt gatgttactg agcaaccagc ctgtcagaag
     7381 tactgttaac gtttcttctt tttgttcttt aatttttgta agtaagtcaa gtccagttat
     7441 tcctggcaaa ccgtaatctg ctatgacaat atcaaaatta tttattttaa aagattctaa
     7501 agcctggtcc gcattgttga atactgaaac tctgttccca agagaatcaa aaacctcgta
     7561 cattatttcc tgtaaagcag gctcgtcctc aacaatacaa atatctttgt cgcgtaattt
     7621 tgcttgaggt acttgctgct cattttgagg aattggagaa atattttcct cgggcaggat
     7681 aattgaaatt gaataatcat ataatgattc ttcttctaat attttggcat ggttttgttc
     7741 agcaagtttt agcagatcaa tgcttgaaat aaatattgaa tcatccttta cttctgaagt
     7801 atcttttttg gtgcgcattg tgattgacaa tgtgctgttc ttttttaaga ttatttcgat
     7861 agttcctttt gaagtgataa cagatgcaac cttgaatatt gttgagagaa gtaagtcgcg
     7921 gaatagatga ggatctccat acatgctgag gagagagaaa tatcgccttt caatagatat
     7981 atatcttcgt ttgctggctt catcaacctt gaattgtatt ttagcaaatt ctatcgcatc
     8041 ttctaccgct tcaacaatat taatattagt tttttcttct aacttatctc ttccaataaa
     8101 ttcttcaatc cgacgtacct gttgtatgcc ttcttcagca gtttttatga ttagtttaag
     8161 tccttcatgc acaggaggtt tttcagtaat ctgcagtaac cattgtgatt tgtttaacaa
     8221 aacattaatg atattattga atgaatggag aaatcccttg gtaaggtcac caatgatatg
     8281 gaatctacta agctgctgta tttgatactc aagtcgttta attcgtgtaa tatcttctaa
     8341 tattatttct acaacaggtt gaaaatcagg tgaatggtta agatagcatg atgcgttaac
     8401 ccacttttca tctttttctt tagttataaa tcgtatttca cctatgcgtt tatatgctat
     8461 agttccatta aatatttgcg atagtgttat acgcattcta ttaaaatcgt aatgattcat
     8521 tgcccggtat aataaattcc tattttgttt taaatcatct acctcataac caataagttc
     8581 ctggattgat gaactggctt ttattatatt gccagattga tcaatctgta aaatgggaaa
     8641 tggggaatat tcataaagtt gcgactcata atttttatca aagtcattta tcaatgtttc
     8701 agttgatttg tcaaatgaca gaatccttcc atcaactatc tgcaaaatta caaaaagtgc
     8761 tacaatagtt ttcccctgaa atacgggaaa aatgtacaat tcagcggata tcctttgatt
     8821 atctttgtta cgaaatacag caaaacattg cctggatatt ccttttaatg cttttaaata
     8881 ggatgttgct atctgtttgc gatattcggg ataaaaggga tgggtaatat caaggcatag
     8941 ctggttcata ttaattaaat cagagaaggg tgtattcaat gctatcactt cgcctgaaat
     9001 gctgataacg catgcaggta attgaaacag gagcaccata tttgatgctg tttgcaaagt
     9061 tgtagtattt atatgtgttg caccactatc ttcactactc atacaaggta ccaccatgct
     9121 taaccatgtc aaaacataag tatttttttg caataaaaaa tataaaaaaa aatagtgtta
     9181 ctgtgaaagt tttgcttgta gtgtatcagg ggattggaag cctgccatct tttctataat
     9241 cttaccattt tcaaagagga gtattgtggg aattgcatca atatggtagt ttttgataat
     9301 ctgctgattt tcatctgcat ttattgtaac aaattttgct tgtgccgatg attgtgaaag
     9361 gtttttcaaa acagcattct gacgcataca taggcggcac catggtgccc agaaatttac
     9421 aactactttg cctctccatt gcagcacttc gcgggcaaag ctttcttgtg taacatcttt
     9481 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncctgcatt atcaacatat
     9601 ttaaaggtga tagtgtaggt acctggtggg aggaataatc tgcttagctg gaaattttca
     9661 ggcaaagtat gccagcaccg caaatcaggt tttatctgtg atagtaaacc tacgccaacg
     9721 ccagcacccg ctaccatacc aattaaacct gcaaagttct ttgtactgtc tgactgttcg
     9781 gctaattttt ttgcagcata tcctgctgca acggatgcag cagctttcac tgcaatgcct
     9841 gcagcaactt ttgcatatat tcgtccataa tcgtcttcca gagtcttcac tgcggtagtt
     9901 gcaatatctt ctaacatgtg acttcgttca atgtccttgc cattaacatt aacaaccaga
     9961 tagttaattt ttccttcgcg ctttacaaat tttggtattg gattttcggc atttttaaga
    10021 gctatcataa ctccggcaat ggtaactcct tcttgtaatg acatgccatt gagcgtcacc
    10081 tgaattgaag atttcatagc ctgatctgaa agaagtgaac cgcgggactt tttaatagca
    10141 cccttcccat tttgcactat aactacaagc tcacctgcat cttttggtat tgtatcacgt
    10201 ttaccaaatt tgccaatcca cattttataa tcgtctgtat cgcctaattg ttttgcaagc
    10261 ctttgtaaat cgcggtaaac aatatccaga gcaggattga gttcataaat ttgcttgtat
    10321 tctatatagg caaattcacg atcattcatg tcgttgtcct gatctgcaat ctgttcaaat
    10381 gcaatagctg tcagatattt tgccattata ttctgcttat acgatttccc tgttgttaca
    10441 cttatatccc ttaataaatc gttaactttt ttaaattcaa cgcgtgcttc atccggtttg
    10501 ctgagcataa ggtagttaat tccaagatac atgtgtatca gcaccctttc aaaatcttca
    10561 ccattataat cagtagttgt ttcgttcagt aacagtgatg cagcctgttt actaacgcta
    10621 atggttatct ggtcggctat cttagctgct tcgataaaaa ctttgttgct gttttcgtaa
    10681 tctcctgcaa cctgcaatat caaaccgcat tccatcatgt ataaaagctg atcttttgat
    10741 tttgtattta ttgcagggag cagtttattg gctgcctcct tatattgacc ggagtaaaat
    10801 aatttttcaa tttcgccaat ttgtttgtta tagtcataac tggcacatgt tactactgat
    10861 accagcagaa ataaaataca aaaagattta agcttttgcc tcatacaaat tactccggat
    10921 taaaatgtta ttttcttttt actggctgat tttaaaaatt cctgttgatc ctgccactca
    10981 accacctgcg ttttcagatt aataagcttt aaggtaacca ctaaatattg aactgtttta
    11041 cttcctacag tgcgcacatt ttcccttacc tcaccggtca aatacatgtt tggtgatttt
    11101 aattcgccaa ccgggatagc gctttcaggg tcaaccatgc cggtcatacc cttttccatt
    11161 tctgaaattg catcagtagt aagcgaggca tctacaaatt ttatgcggcg tttaataagg
    11221 ttggttgtta tttcatcagc aagcattttt gtatcaatgt gttcagatgt tttattacga
    11281 atttgtttta cttctatgta ggcgggtttt ttccattcat cttttaaaaa tgaatacatt
    11341 gactcaacca tcgtattaac tgtcatctta atttcttttg gtccccattc acgtgagccc
    11401 tcagctttac ttgcatcttt atattgcacg ccaccgccac aggctataaa accaataata
    11461 aaaattatca atagcaatga aagctttttc ataaatataa cctcctgaat tattataaag
    11521 attatggctt gttttgcata acaaaaagaa actaatgaaa taatcactgc tatagatagg
    11581 cagtaaagtc aagaaaaaaa tggtggttat tattgtagca tggaatgtat caaagcatct
    11641 attgtatttg cagtatcatg tggttcagtg tagttaatag atgcagtaaa tgatatattt
    11701 ttgatactgt gctggtattc aggatcataa tggtgctgta ataaaaattg tataaattct
    11761 aaatacttac catggcagaa caactgtttg caatactgcg tttttgtttt acccagagct
    11821 ggttgtaatt tgtccactat agcaatgata gcttcttcag tgatatatgg catatattcc
    11881 tgatagatat gttgtattct gacatcaatg ggagtatcaa gccataatgc tatccccttt
    11941 tgcatggctt ccagaattat tggcggtatg tggcagttcc caattttgcg tgattcgccc
    12001 tcaagcaagc agtagggcca tacgttaatg gtttgcaata tatgaactat cctgctttca
    12061 aaatattttt gtgaatgatc tggcatcccc agactgccaa ataacgagct tcggttgcct
    12121 gcacagccct caaggtcaag ggaataggga ttgtggtgga gtatctttgt tttaccggag
    12181 ccagcaagcc cataaactgt aactacaggg atggtaaagt aatagtttgt tagatagttc
    12241 cttataaact ggcgataggc tttatatcca ccttccagtt taaagagatt aaatccaata
    12301 ctgtttaaaa atgatactac tgattcggag cgcattcctc ccctgaaaca gtaaacaata
    12361 atggtggaac tggattgtga taataatgaa aaaaatgaaa ccataggctt caattttggt
    12421 gaaacgattt ctatcccttc catgagcgct tcatctctgc ctctggtatg atataacgtg
    12481 ccaatatatt cacgctgttc atcatcaaga agcgggtagt tgactgcatc agggatatga
    12541 tcgttagtgt attcaacagg tgctcgtaca tccacgtata ctttatcagt tatggaacaa
    12601 ctatcgaaaa atgtgataga tggtatttgc ataggttttt atcgttttat gacatgcgta
    12661 tggttactgt gccaaactgc agcaattgtt cgatggctgt gaacagtaca tccatataat
    12721 gactcatgga tggtgcaaac agcaatgggt gaaaattgtt ctttgaaaca aaagtcgtca
    12781 atagctgata tccattatat gcttctactt ccatgggata gtcatcggta aatatagctt
    12841 tgcaataatc tacaggtata gcatagaccg cgtttacctc attatcatta aatgaatagg
    12901 ctgtcaacgg ttcatctgac atatacaaat ataccctctg gaattcgcgg tgaaaaagag
    12961 tagcattgtt ttcttcatac cggaaatatc ctaaatcaat gagtttatta tcatccggta
    13021 ttatccctat ctcttcatat gcctctttat ggattttatt ggtgtctaat ccatagggta
    13081 catgcccacc aacggttata tctaaataac caggatacat atctttatgg gaagcacgtt
    13141 tctggaacac aagctgtgga ataggtatag aacgcacaat ccataaatga acaccttcat
    13201 gggctatgcc gcattgatgg gcaatacttc gtggtataga ttttcctgtt gggatacctt
    13261 tttcccaatc ccattcttct aacatttctt ctggttgtag tgtcattgta attatgttcc
    13321 ccctctgtaa atagaatact gtgtcatttt gagggatgta gttcagaaga atctgttctt
    13381 ttaaaaaact acgagattct tcacttcgct cacaatgaca ttcccgtatc acgggcttct
    13441 tcactttgac tttgctcagt acaggcggct ttacctcaga atatcagtaa cactacatgt
    13501 tcattatatc aattcatcaa caaacttcta aaaactggat tgtaatattg tatgggtaca
    13561 tgctatgctc ctcagttgtt t
//
LOCUS       DAOOJH010000063        13531 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4285_length_13531_cov_10.762986, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000063 DAOOJH010000000
VERSION     DAOOJH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_13531_cov_10.762986"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(230..877)
                     /locus_tag="PLJ75_08715"
     CDS             complement(230..877)
                     /locus_tag="PLJ75_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015194725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HPD05333.1"
                     /translation="MEQKYYQYILFCDFDGTITAEETFVGMCQKFAPDTFHEIGKRMW
                     ERQLTIREGVRAVIESLPSSLYPAMLAYIDAIDMRPGFPEFLCYCNNHSIPVVIISGG
                     LYDCVVRKLGDLINKVEAIYAAKVDASGDYLKLYSDYEYEGELVAKAVIMDNYRAQCR
                     IAVGDGPTDIEMAKHADIVFARSMLKQFLPEDKKYYEWDIFFDVIETLSVIIDKK"
     gene            complement(914..2494)
                     /locus_tag="PLJ75_08720"
     CDS             complement(914..2494)
                     /locus_tag="PLJ75_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05334.1"
                     /translation="MDTQYTSRQILEIKSSGTYIILFILIALIAHVFQFIAKVDLFEI
                     LKLSFASIICLLIAYSIYKRKKTLKRTEILEWLLGFITLTIPLAAKFAYATTYDWTFA
                     LESYNSSILLIIFMVLLQLYFNKRLVAFYSSYAIVTWVAFLLLAIAHGADIHWDATID
                     GKPIHGFIIFREIFMIVITGIFGILAYKNIQIVEQYDDRSTRQLSIIHGQSEKQKQLN
                     EIIKEKVSDLLQQVQRQDELTIMFNDKMQNQASTFEEISATLEELLGSAESIAHSSEE
                     QVGGNTMMETIVSEFKIIKNETRQNLDKTIHEVDNVVAQTGSANERIRDVESTINEIR
                     NQSQKIAETIGVIVDIADRINLLSLNASIEAARAGDYGRGFAVVADEIGKLAYQTSES
                     IKDIESVLMQSTKTTEKGVEVIRTTAAMLKELIAKMAESSSKIKILQDSTTVEEKYIK
                     NIIAQMEKNVELAKNIGTGTEEQKAAIESSARAIEHMNEVVSQMVDEIQKLAMTSRSI
                     LDNATALLEQSKGDSSSE"
     gene            complement(2677..3522)
                     /locus_tag="PLJ75_08725"
     CDS             complement(2677..3522)
                     /locus_tag="PLJ75_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD05335.1"
                     /translation="MVNLSQPVLKTISSRYSCRTYTNEELKDEDYDYLQQCIAAISTL
                     SGTTLRITLIHNNRVKLPQKIGTYGFIKNPQGFIAATTTDTKEGLVDFGYALEQMVLA
                     ATERSIGTCWLGGTFTRGPFNQIMGLLPHEIVPAVISLGYPADKKRMFERSVRYIAGS
                     DTRKKADELFFEHTWNNPYKGSEPFITLLELVRLAPSASNKQPWRILFNNNAFHFYLE
                     RTPGYYDRNRALLGFHDLQMIDMGIALCHFHYGTIDYKIDGRFAVTHHPAIDKREYII
                     SYVLG"
     gene            3747..4262
                     /locus_tag="PLJ75_08730"
     CDS             3747..4262
                     /locus_tag="PLJ75_08730"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HPD05336.1"
                     /translation="MKRISLAFVVIMLVTSVAFGGEPKGRGHDCPDMQMKKGPIFGDV
                     ERLKQELGLSDAQVDKIIAINKQYGMKMLDIREKLAPNVIQLKKLLLQDDVDINAVRA
                     KLKEIADLRVELHLLRIQHVIDIEKQLNKEQLNKLRNYKRAEMKKMKKMHPGGDCGPM
                     GPEGPDDGPMM"
     gene            4281..4772
                     /locus_tag="PLJ75_08735"
     CDS             4281..4772
                     /locus_tag="PLJ75_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPD05337.1"
                     /translation="MDASEFTAIVHETKDGVLRAVQKHLAAQFYEAIDDVVQETYLRA
                     YKSLAAGKFEGRSTLDSWLYRIAANESKRMNGKLLREQKKLQKLSRATTAYSPAIDCE
                     ELSLMPVIEMLPDKYKDVVKLKAQGNNEWEIATKLNIPKGTVKSRLSRGKDLLLRLLQ
                     EEL"
     gene            4769..5110
                     /locus_tag="PLJ75_08740"
     CDS             4769..5110
                     /locus_tag="PLJ75_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05338.1"
                     /translation="MNNYNLLFNEAIQQRLHSSQWDKAMALQVMAKVKKRNRLYTAFA
                     SAAAMILFAAAVTLWQYNSDDSTNNAMYSFVHAQVNGVYTTVFPEANNADMLYGDDRV
                     GDIIETSLFER"
     gene            complement(5112..5813)
                     /locus_tag="PLJ75_08745"
     CDS             complement(5112..5813)
                     /locus_tag="PLJ75_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05339.1"
                     /translation="MEHSGRSFIASPETCPYLGNRTKLYRYCILYSLSQDDLDHLLVQ
                     GWRHFGYTFFKPECPGCMLCIPVRVKTFEFKPSKSHRRTQRKNIQTTVKVSELSLTRR
                     IYDIYGDHSLSRFGQPPSRQFLISNVLDICTNTYQIEYYIHSTLFAVDYIDETNKALN
                     TIYCIFHSDFAHLSPGIFSILYAIQLAQDKNLPYLYLGYYVPGCTVMEYKISFHPYQW
                     YSWQKKQWLDEPVQL"
     gene            6498..7850
                     /locus_tag="PLJ75_08750"
     CDS             6498..7850
                     /locus_tag="PLJ75_08750"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HPD05340.1"
                     /translation="MIMVESFLTREKYRFNYVARLSWSLILFTIVVYNFKGILLILST
                     SYLVYSILWAIAVELDAPLIHYQNTRYIRITFDAFFYTMVIYLTGGINSFLLLSYILF
                     IMMASLYTTNRYGIYAIVICMIYYNAMLAMIYYNIIPSVNILANEIQSSVAITTTSVM
                     FTNFILILVSVILHRITYRLYFKLNEKTNELQTERNVLKKWNELIEQDLNLARKIQIK
                     LIPNKDPYPFIHSLYVPMEAVGGDFYDFIEFKESKDIGIFVSDVSGHGVHAAFITSMI
                     KTLILQAGVIRNNPAELLRYLNKMLYGITADNFITAFYGIYNPLNHSITYASAGHNPP
                     YVISGRNIIQLNKSKGIPLALENNDYLFSRGKDYKNVTYGMNSDDRIVLYTDGLIEEH
                     VDDDHYKNFGELMRQEILPYAQSYSSKEIIDTLYTSLKKKSGKEHFNDDVCIICMEIH
                     "
     gene            8308..9891
                     /locus_tag="PLJ75_08755"
     CDS             8308..9891
                     /locus_tag="PLJ75_08755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPD05341.1"
                     /translation="MSTTNRKEKVYIFDTTLRDGEQAPGFSMNIDEKLHFADQLVKLG
                     VDIIEAGFPVSSPGDFEAVKKIAERVKGVQVAGLARANRKDIDAAWDALRNAENPRIH
                     IFISSSDIHINYQFKKTREEVLAIAVDSVKHARNYMPNIEFSPMDATRSDWNYVAQMV
                     EAVIHAGATTINIPDTVGYAMPSEFHQFITFLFNNVPNIDKAIVSVHCHNDLGLATAN
                     TLAAIEAGARQVECTINGIGERAGNTAMEELVMALKVRGDKYNVYTDINTKQIVPTSK
                     LLTHITGVNVQPNKAIVGANAFAHESGIHQDGMLKNAMTYEIMKPEDVGITKSALILG
                     KHSGRHAVIARLNNMGYNLSDEELDRFFKLFKVLADKKKQIFDEDLEVLVGEAVYKVK
                     GRYRVTNVQISTGMYSPPMTLVTIKDRNDEGVESFDVAHGNGGVDAGINAVKKITGTN
                     AKIESFNLVAITGGSDALCEVTVTVVEEIDGKQLKVFGNGVNIDISVAGILSFVDALN
                     KLDYIKQRNHIGEKIEVQL"
     gene            9915..11552
                     /locus_tag="PLJ75_08760"
     CDS             9915..11552
                     /locus_tag="PLJ75_08760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPD05342.1"
                     /translation="MPLTLDEAKKRGWNSIDIILVTGDAYIDHPSFGVAVIGRVLEKA
                     GYTVGIIAMPNFMDPSSIEVMGRPNLFFGVTSGNIDSMLARFTAFKKIRNDDPYVPGG
                     MGGVKPSNAIITYCNLIKQRFKDVPIVIGGIEASMRRLVHYDFIQNKLRRSILVDSRA
                     DILVYGMGESQTVEIANRLSGGQSMEGISGTVTIARNKPLNAFLLPSEKEATESNEAL
                     ITMYKQFYQNFQSKVIIQESGGRYITHYPPPQISQEQLDSYYDVPYIRKPHPVYTRTI
                     PAYTMIQYSINAHRGCAGGCSFCTLTLHQGKRIVSRSRDSIKQEITAIAAKKAIHITD
                     IGGPSANMYGMECSIGGCNRESCVFPAVCKHLKINTRGWIDLMDEALTLPNVKKVSVG
                     SGIRYDIFMRSKDCLHELSHFIAHFVSGQLKVAPEHTDSTVLRAMRKVPLYPLAEFVK
                     AFNHECKRQAKEYYIVPYLMTCQPGSTDSSVYRMRSEIEAIFGYIPFQCQAFIPLPMT
                     LSSIQYYTGSDPLTGELFFVEKNTKARRKQHGILMKR"
     gene            complement(11811..12809)
                     /locus_tag="PLJ75_08765"
     CDS             complement(11811..12809)
                     /locus_tag="PLJ75_08765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPD05343.1"
                     /translation="MSLEPETLINIINIQTEVVKLGLDLAGVMDLVVQRAQQLTHADG
                     AVIELAEDDYMVYRAASGTAEKQLGLRLHKETSLSGLCVQVGHALQCSDSEEDPRVDK
                     DACRKVGLRSMVVSPLKYGDIVVGAIKVLSSKPNFFDDTATHVLDLMSELIAASMYNA
                     ERYSSNELFHRATHDQLTGVANRALFYDRLRRRLIQAQRLKEKFSIIILDMDELKQIN
                     DNWGHRAGDTAIQEFSLRIKKATRQTDTVARLGGDEFGIIVHAIKNDDDIAIVTQRIE
                     NALTQPFKFEGKKIPLQASMGWALFPNDGEELEVLIDNADQRMYTMKRMHKNNPKS"
BASE COUNT         4159 a         2421 c         2738 g         4213 t
ORIGIN      
        1 tttaaagttt caggtagtga agcaatagcc ataacatttc aatgggaaat gagaattttt
       61 gtggaaaaat agagagagat tcccatggct gtgtgctcgc ctgcaacggc ggtcgccttc
      121 cgtccgggga aggctactgg gaaagtatcc cagccgaaca gggaggtatt gtatctttgg
      181 atgcgggcgc atgatagggc agtctgccgt taatctatca ttcaataaat tatttcttat
      241 caatgatgac agaaagagtt tcaataacat caaaaaaaat atcccattca tagtattttt
      301 tgtcttcggg taaaaactgt ttcagcatgc tacgcgcaaa gactatatca gcatgttttg
      361 ccatctcgat gtcggttggt ccgtcgccaa cagctatcct gcattgtgcc cgatagttat
      421 ccataatgac agcttttgcc accaactcac cttcatactc ataatcagaa tacagcttta
      481 agtaatcgcc gcttgcatca acctttgcag catagatagc ctctacttta ttgattaaat
      541 ctcccagctt acgaacaacg caatcatata agccgccaga gatgatgaca accgggatag
      601 aatggttatt gcagtaacat aaaaactcag ggaaaccggg cctcatgtca atagcatcaa
      661 tataggcaag catcgctgga tatagtgaag aaggtaatga ctcaataact gcgcgtacac
      721 cttccctgat ggtaagctga cgctcccaca tccttttgcc aatttcatga aaggtatccg
      781 gtgcaaactt ctggcacatc cccacaaatg tttcttctgc agtgatagtg ccatcaaaat
      841 cacagaatag tatgtactga tagtattttt gttccattgt ttgtttgata gtttctctat
      901 aacaggcacg ctgctactca gatgagctat cgcccttaga ttgttcaaga agagcggttg
      961 cattgtcaag aatactgcgt gatgtcattg caagcttttg aatttcatca accatctggc
     1021 tgacaacctc attcatatgc tcaattgctc tggcacttga ttcaatagct gccttttgtt
     1081 cttcggtgcc cgtgcctatg ttctttgcta attccacatt tttttccatc tgggcgatga
     1141 tgtttttaat gtatttttct tcaacggtgg tgctgtcctg caaaatttta atttttgatg
     1201 agctttctgc catttttgca attagctctt tgagcattgc tgccgtagta cgaattactt
     1261 caacaccttt ttcggtagtt ttagtgcttt gcataaggac actctcaatg tctttaatac
     1321 tttcagatgt ttgatatgct aatttgccaa tttcatcggc aacaacggca aaacctcttc
     1381 cgtaatcgcc ggcacgtgct gcttcaatgc ttgcattaag ggataaaagg ttaatcctat
     1441 cggctatatc aacaataaca ccaatggttt cggcaatttt ttggctctgg ttgcgaattt
     1501 cattgatagt tgattctaca tcccgtattc gttcatttgc gctgccagtc tgagcaacaa
     1561 cattgtcaac ttcatgtatg gttttatcca gattttgacg ggtttcgttt tttattatct
     1621 taaattcgct tacaatagtt tccatcattg tattgccgcc aacctgctct tcggagctgt
     1681 gcgcaatgct ctcggctgaa cctaataact cttcaagtgt tgctgatatc tcttcaaagg
     1741 ttgaagcctg gttttgcatc ttatcattaa acatgatggt taattcatcc tgacgttgta
     1801 cctgttgcag taaatcagat accttttcct tgattatctc attcagttgt ttttgctttt
     1861 cagattggcc atggataata gatagttgcc tggtggacct gtcatcgtat tgttcaacaa
     1921 tctgtatgtt tttgtacgcc agtattccaa atatgccggt aattactatc atgaatattt
     1981 ctctaaaaat tataaagccg tgaattggct tcccatcaat ggttgcatcc cagtgaatat
     2041 ctgccccatg agctatcgcc agcaataaaa aagcaaccca ggtaacaatg gcatatgaag
     2101 aataaaacgc tacaaggcgt ttgttaaaat aaagctgtaa taaaaccatg aatataataa
     2161 gtaaaattga gctattgtat gattccaggg caaatgtcca gtcataggtt gttgcatacg
     2221 caaacttagc agctaatgga atggtcaggg ttataaagcc taatagccat tccagaattt
     2281 cagttcgttt aagtgttttt tttcttttgt atatactata ggcaataagt aaacagatta
     2341 tactggcaaa tgaaagcttc aggatttcaa aaagatcaac ttttgctatg aactgaaata
     2401 catgcgctat caatgctatc aaaataaata aaattatata tgtcccactg gatttaattt
     2461 ccagtatctg tcgggatgtg tattgtgtat ccattgtgac ctctttacta ttgtaaggtt
     2521 gtccaaactt tatacaaaga atacaacata acaatgaaaa tagcaaattt aaaaaaagaa
     2581 aaatacagca attaaaactt ctaaactatt caaaatagaa tagacatatt ttgcctacta
     2641 taataatagc acatcatacc actgtatgca taaattttat cccaatacat aactgatgat
     2701 atattcgcgt ttatctattg caggatgatg tgtgacagca aaccttccat ctattttgta
     2761 gtcaatagtt ccatagtgga aatgacacag tgcaataccc atatctatca tctgaaggtc
     2821 atggaatccc agtaaggctc tgttcctgtc gtagtatccc ggtgtacgtt ccagatagaa
     2881 atggaatgca ttgttattga aaaggatacg ccatggctgt ttgttggatg ccgatggcgc
     2941 aagccgtacc aattccagca atgttataaa aggctctgac cccttatagg gattgttcca
     3001 tgtatgttca aagaacagtt catcagcctt tttccgtgta tcagaaccag ctatataccg
     3061 tacagagcgt tcaaacatcc tttttttatc agctggatag cccagcgata taacagcagg
     3121 tacaatctca tggggcagta atcccattat ctggttaaat gggcctctgg taaaggtacc
     3181 acccagccag catgttccaa tactgcgttc tgttgctgct aataccatct gctcaagagc
     3241 atatccaaaa tctaccagcc cttcctttgt atcagtagtg gttgctgcaa taaatccctg
     3301 gggatttttt ataaatccat acgtaccaat cttttgtggt agctttaccc tgttattgtg
     3361 tatcagcgtt attcgcaggg tagtgccaga taatgtgctt attgctgcaa tacactgttg
     3421 cagataatcg tagtcttcat cttttagttc ttcattggta taggtacggc atgaatagcg
     3481 gctggatatt gttttcagta ctggctgtga aaggttaacc attttttacc tcactataat
     3541 aaaatataac aaagaataaa aaaattttga ttatttgtag tgaactgcta accctatatc
     3601 cctatatact gaaagatagc ataattgcaa taattttata gtagaaaaat gaccatttat
     3661 ggaacctttc gtgtttttat caggtctaat agctttgttg ggaagaataa gctttcacgt
     3721 ttaattttat aatgaagggg atggctatga aacgaataag tctggcgttt gttgtgataa
     3781 tgctggttac atcagtagca tttgggggcg aacctaaagg aagaggacat gattgtcctg
     3841 atatgcagat gaaaaagggc cccatttttg gtgatgtaga acgtttaaag caggagcttg
     3901 gattatctga tgcacaggtt gataagatta ttgcaatcaa caaacaatat ggaatgaaga
     3961 tgcttgacat acgggaaaaa ttagcaccca atgttattca gctaaaaaag cttttgcttc
     4021 aggatgacgt tgacattaat gcagtgcgtg ctaaactaaa agagatagca gatttgcggg
     4081 tagagctaca tcttttacga attcagcatg tcattgatat tgaaaagcag cttaataagg
     4141 aacaacttaa caaactaagg aactataaaa gagcagagat gaaaaagatg aaaaagatgc
     4201 accccggcgg tgattgtgga ccaatgggtc ctgagggtcc tgatgatggc ccaatgatgt
     4261 aggggcaaag gcttatcctg atggatgcaa gtgaatttac ggcaatagta catgaaacaa
     4321 aggatggtgt gctgcgagca gtgcaaaagc acctcgcagc acaattttat gaggcaattg
     4381 atgatgttgt gcaggaaaca tatctacggg catataaaag ccttgcagcg ggtaagtttg
     4441 aaggaaggtc aacgcttgat tcatggctat acaggattgc tgccaatgag tcaaaacgca
     4501 tgaatggtaa attattacgg gagcagaaaa agctacagaa attatcaagg gcaacaaccg
     4561 catattcacc tgcaatagat tgcgaggaac tatcgcttat gccagtcatt gagatgcttc
     4621 ctgataagta taaagatgta gtaaaactga aggcacaggg taacaatgaa tgggagattg
     4681 caacaaagct caatattcct aaaggcactg ttaaatcccg actgtcacga gggaaggatt
     4741 tattattacg cttgttgcag gaggaactat gaacaattat aatctacttt ttaatgaagc
     4801 aattcaacag cggctgcaca gttcacagtg ggataaagct atggctttgc aggtaatggc
     4861 aaaggttaaa aaaagaaata gattatatac tgcttttgcc tcggcagcgg ctatgatact
     4921 atttgcggct gctgttaccc tgtggcagta caattctgat gatagcacca ataatgctat
     4981 gtacagcttt gtacatgctc aggttaacgg ggtgtatacg actgtttttc ctgaagcaaa
     5041 caacgctgac atgctgtatg gtgatgatag agtgggtgat attattgaaa catcgttatt
     5101 tgagcggtag tttatagttg cacaggttca tcaagccact gtttcttttg ccagctatac
     5161 cactgatacg ggtgaaatga gattttgtat tccataaccg tacagccggg aacataatat
     5221 cctaaataaa gataaggtaa atttttatct tgagccagtt gtattgcata caggatgctg
     5281 aagataccgg ggctcaagtg tgcaaagtca gaatgaaaaa tacaatagat tgtattgagc
     5341 gctttgttag tttcatcaat gtaatcaacg gcaaagaggg tggaatgtat gtagtattca
     5401 atttgatagg tgttggtaca tatatccagg acattgctga ttaaaaattg tcgagatggc
     5461 ggctggccaa aacgcgataa tgaatgatcc ccataaatat cgtagatcct tctggttaag
     5521 gaaagttcac tcacttttac ggttgtttgt atattttttc gctgtgtcct gcgatggctt
     5581 tttgatggtt taaattcaaa tgttttaacc ctcacgggta tgcacaacat acagccgggg
     5641 cattcgggct taaaaaatgt atatccaaaa tggcgccaac cctggacaag taaatggtca
     5701 agatcgtcct gtgataggct atagagtatg cagtaacggt ataattttgt tctgttgcca
     5761 agataggggc atgtttcggg gctggctata aatgatcttc cagagtgttc cataattatg
     5821 tactataata acaaaagtat tgttgtcaat tgaatggcaa tagtgcttgc aataaactat
     5881 atccgtaaag agccaatgca tacgctctaa tcaataatta taagtatggc gatagttact
     5941 ctaaaacaaa aaattgttac ataaacgatt ggttgttgca tgaattattc attcagtttc
     6001 atcctccatg agatcctgaa acaagttcag gatgacgttc tcaccatggt cattctgagg
     6061 gacgtagtcg cgaagaatct attcttttat acaacgacga gattcttcac tttgctcaga
     6121 atgacattct ccgttcagga tgactgtcgc caactatttt catcccttat ggatggccgg
     6181 aagacagccc ctataaactt ctcaatactt tcaatagtat ataaacgtaa caggtatttg
     6241 aaataatagt aatagcgtat ggtacgatgt tatgataaaa gtgtcaatta ttttccagag
     6301 gtttactcat gatagttttt tatttgattt tctctattta tttgttaaac ttataaaaaa
     6361 ttgttttctt aatactatac agtatagtat gaacacctta aacaatcagg tttttttatt
     6421 atatacgatg gtatacaata ttttactgat gcttgtgcca acaagggaat gtccaaaaac
     6481 taattattaa aggttccatg ataatggtag aatctttttt aaccagggaa aaataccgtt
     6541 ttaattacgt cgctcgcctg tcgtggagct tgatactttt tactattgtt gtatataatt
     6601 ttaaagggat attgttaatt ctttcaacgt catatcttgt ttacagtatt ctatgggcaa
     6661 tagcagtaga attagatgct ccattgatac actatcaaaa tacgcggtat attcgtataa
     6721 catttgatgc atttttttat accatggtaa tttatctaac aggtggtata aactcatttt
     6781 tgttgttaag ttacatacta ttcattatga tggcttcact ctatacaacg aatcgatatg
     6841 gtatatatgc gatagttatc tgtatgattt attacaatgc aatgctggct atgatatatt
     6901 ataatattat tccatcggta aacatccttg ccaatgaaat acaatcttcg gttgctatca
     6961 ccactacctc ggtgatgttt actaatttta ttctgatttt ggtgtcggtt attctgcacc
     7021 ggattaccta tagattgtat ttcaaactta atgaaaaaac aaatgaactg cagacagaac
     7081 gaaatgttct taaaaaatgg aatgaattaa tagaacagga tttaaacctt gcccggaaaa
     7141 ttcaaatcaa acttataccc aataaagatc cttatccatt catacattca ttatatgtgc
     7201 ctatggaagc tgtcggcgga gatttttatg attttattga atttaaagaa tcaaaggaca
     7261 ttggcatatt tgtgagtgat gtttccggac acggagtaca tgcggcattt attacctcga
     7321 tgataaagac tcttatatta caggcagggg taatcagaaa taatccggca gaattattgc
     7381 gctatcttaa taaaatgctg tacggtatta cggcggataa ttttattaca gctttttacg
     7441 ggatttataa tcctttaaat catagcataa cctacgccag tgcaggtcat aatcctccct
     7501 atgttatttc aggaaggaat ataattcagc ttaataaaag taaaggaatc ccattagcat
     7561 tggaaaataa tgattatctg ttttcaaggg gaaaagatta taaaaatgtg acatatggca
     7621 tgaactcgga tgaccgaatt gttttatata ctgatggatt gattgaggag catgttgatg
     7681 atgaccatta taaaaatttt ggagaattga tgcggcagga aatactgccg tatgctcaaa
     7741 gctattcaag taaagaaatt attgatacat tgtacaccag tctgaaaaaa aagagtggaa
     7801 aagaacattt caatgatgat gtgtgtatca tttgcatgga aatccattaa aaggcttaaa
     7861 aaacatattt tatattttat aggcagtaga gttatacctt aatatttttt atcctaaaac
     7921 cgatagttaa atagcagtga aaaaattaat acggcaacag caaaatatat tgacaatata
     7981 tcaggatgcg attaattgta tattaaatta atatattaaa ttgatgctat taaaaagtaa
     8041 aatatgatga atactgtatc acaaaacctt aacatttttg caaatctatt gggactacta
     8101 ctagtgggga aaaccgcgac aggggtttcg tgaaaggtat tggtatacta aaaaaattaa
     8161 tgaccgccac ggaaaccccg tggcggtttt attttatgta tggaggtata cagggatgaa
     8221 ttcccagcga ttgctactat ataccaaaaa acatctatta ttatttatta ttgcattata
     8281 tctattgact gaataaggag aaaaagtatg tcaacaacaa acaggaaaga aaaggtttat
     8341 atttttgata caacgttacg ggatggtgag caggctcccg gttttagtat gaacatagat
     8401 gaaaaactcc attttgccga tcaactggta aaattggggg tggatattat tgaggcagga
     8461 tttcctgtat catcccctgg cgattttgaa gcggtaaaaa aaatagccga aagggtaaaa
     8521 ggggtacagg ttgcaggatt ggcacgggca aatagaaagg atatagacgc cgcatgggat
     8581 gctttgcgca atgctgaaaa tccgcgtatt cacatcttta tatcaagctc agacatccat
     8641 attaactatc aatttaaaaa aacacgtgaa gaggttctgg ccatagctgt tgattcggta
     8701 aaacatgcca gaaattatat gcccaatatt gaattttcgc caatggatgc aacaaggagc
     8761 gactggaact atgttgcgca gatggtagaa gcggtgatac atgccggtgc aacaacgatc
     8821 aatattccag acacggtggg gtatgccatg ccatcagaat ttcaccagtt tattacattt
     8881 ctttttaaca atgtgcctaa tatagataag gcaattgttt cagttcattg ccataatgat
     8941 cttggacttg ctactgccaa tacactggct gctattgaag caggtgcacg tcaggtggaa
     9001 tgtacaatca acggtatagg cgagcgggca ggtaatacag ccatggaaga gctggtaatg
     9061 gcactcaaag ttcgtgggga taagtataat gtatatactg atatcaatac taaacaaatt
     9121 gtgccaacct caaaattgct tactcatatt acgggtgtca atgtacaacc aaataaagcc
     9181 atcgttggtg ccaatgcttt tgcacatgaa tcagggatac atcaggatgg tatgttgaaa
     9241 aatgcgatga cgtatgaaat tatgaaacct gaggatgttg gcattacaaa atctgctctt
     9301 attttaggga agcactcggg gcgccatgct gtcattgcaa ggctgaataa catgggatat
     9361 aatcttagcg atgaagagct ggatcgtttc tttaaacttt ttaaagtgct tgctgacaag
     9421 aaaaagcaaa tttttgatga ggaccttgaa gtactggttg gcgaagcagt ttacaaggtc
     9481 aaagggcgct acagagtaac caacgtgcag atatctaccg gtatgtattc acctccgatg
     9541 acactggtta ctatcaagga tagaaacgat gagggagttg aatcatttga tgttgcgcat
     9601 ggcaacggtg gtgttgatgc gggaataaat gcagttaaaa agattaccgg gacaaatgcc
     9661 aaaatcgaat catttaattt agtggctatc accggtggtt ctgatgcatt gtgcgaggtt
     9721 acggtgacag ttgttgagga aattgacgga aaacagctta aagtttttgg caatggcgtg
     9781 aacattgata tatcggtggc aggtatcctt tcttttgtgg atgctttgaa taagctggac
     9841 tatatcaaac agcgcaacca tattggcgag aagatagaag ttcaattata agataatgga
     9901 agtatacatt gtttttgccg ttaactctgg atgaagcaaa aaaacgtgga tggaacagta
     9961 ttgacattat tctggtgacg ggtgatgcat atattgatca tccgtcattt ggtgttgctg
    10021 ttattgggcg ggtacttgaa aaagcgggat atacagtggg tattatagcc atgcccaatt
    10081 ttatggaccc atcatcaatt gaggttatgg gcaggccaaa cctttttttt ggtgttacca
    10141 gcggcaacat tgattcaatg cttgcgcggt tcactgcatt taaaaaaata cgcaatgatg
    10201 atccctatgt tcccggcggc atgggcggtg taaaaccgtc aaatgccatt attacctatt
    10261 gcaatctgat caaacagcgt ttcaaagatg ttcccatcgt cattggtggc atcgaagctt
    10321 caatgcgaag attggtgcac tatgatttta ttcaaaataa attacggcga agtattcttg
    10381 tcgatagtcg ggctgatatt ttagtctatg gcatgggaga gtcccagacg gtagagattg
    10441 ctaaccgcct gtcagggggt cagagcatgg agggtatttc tggaacagta actatcgcca
    10501 ggaataagcc tttgaatgcc tttttacttc cctctgaaaa ggaggctacg gaatcaaatg
    10561 aagcgctgat aacgatgtac aaacagtttt accagaattt tcaaagtaag gttatcattc
    10621 aggaaagcgg tgggcgatat attacccatt atccgccgcc acagatttcg caggagcaat
    10681 tggattcata ttatgacgta ccatatattc gaaaacctca tcctgtgtat accagaacaa
    10741 taccagctta cactatgatt cagtattcca tcaatgctca ccggggttgt gccggtggat
    10801 gctcgttttg taccttaacc ctgcatcagg gcaagaggat tgtatcgcgt tcacgggact
    10861 ccattaagca ggagattacg gcgattgctg caaagaaagc aattcatatt accgacatag
    10921 gtggtccgtc ggcaaacatg tatgggatgg agtgcagcat tggcggttgc aacagagaaa
    10981 gttgtgtgtt tcctgcggtg tgtaaacatt tgaagattaa tacccggggc tggatagatt
    11041 tgatggatga ggctctgacc ctcccaaatg taaaaaaggt atctgttggt tcagggatac
    11101 gctatgatat ttttatgcgg tcaaaagact gcttacatga gctatcgcac ttcattgcac
    11161 attttgtaag cggacagctt aaagtagctc cggaacatac cgatagcacg gtgctcagag
    11221 ctatgcgcaa ggtgccgtta tacccccttg cagaatttgt aaaggcgttt aaccatgaat
    11281 gcaaacggca ggcaaaggaa tactacattg tgccctacct tatgacctgc caaccgggca
    11341 gtaccgatag ctctgtgtat aggatgcgca gtgaaattga ggcaatcttt ggatatatcc
    11401 cgtttcaatg tcaggcattt atcccgctgc ccatgacctt atcgtcaatt cagtattata
    11461 caggctctga ccccttaacc ggagagctgt tttttgtaga aaagaatact aaagcacgaa
    11521 ggaagcagca tggaattttg atgaaacggt aaagcaacga atgggggaga gcatatgtgc
    11581 actttcttgt actggattat gccaatattt cattatcgcc agaaggttta ctctcattgt
    11641 ataattgtat agtatctcgg gattaattcc tgctctattc ttccatttaa tactatcgaa
    11701 acttacagcc gggaaatttc aactatttgt aatgtgtact atatgataaa ttatatacaa
    11761 ttgctataaa aattaaaatt ttattatttt attaattgta tagtaatcct ttaactttta
    11821 gggttatttt tatgcattcg tttcatggta tacattcgct gatcagcgtt gtcaataagt
    11881 acttctagtt cctcaccatc attgggaaat agtgcccaac ccatacttgc ttgcaatggt
    11941 attttcttgc cttcaaattt aaaaggctgc gtaagtgcgt tttcaattcg ctgagttaca
    12001 attgcaatgt catcatcatt tttgatggca tgtacaatga tgccgaattc atcaccacct
    12061 aatctggcaa cagtatccgt ttgtcgtgtt gcttttttta tacgcaatga aaattcttga
    12121 attgctgtat cacctgctct atgtccccaa ttgtcattta tttgtttcaa ttcatccata
    12181 tccagaataa taattgaaaa tttttctttt aagcgttgtg cttgaataag ccgccgtcgc
    12241 aatcggtcat aaaaaagtgc tcgattagct actccagtaa gctggtcatg ggtagcacga
    12301 tgaaaaagct catttgacga gtatctttcg gcattataca ttgaggcggc tattaattct
    12361 gacataaggt caagaacatg agtagcggta tcatcaaaaa aatttggttt agaagataaa
    12421 actttaatgg caccaaccac tatatctcca tattttaaag gagatacaac cattgaacgc
    12481 aaaccaactt ttcgacaagc atctttgtca acacgggggt cctcttctga atcgctacat
    12541 tgtaatgcgt ggccaacctg tacacataat ccggaaaggc ttgtttcttt gtgtaaacgt
    12601 agccctaatt gtttttcagc agtacctgaa gcagctcgat ataccatata atcatcttcg
    12661 gctaactcta ttacagctcc atcagcatgg gttaattgct gtgctcgttg tacaaccagg
    12721 tccatgacac cagcaaggtc aagaccaagt ttcacaactt cagtctggat atttatgata
    12781 ttaattaatg tttcgggttc taagctcata actgtatgat agtgcatacg tcataaaagt
    12841 atctgtcagg cgtttgcctc attgccatag tgaacctata gagtaaatta ttaacaaacg
    12901 tttttttcct gtcaaccaca tttaaagcat agcaggatgc tgaagaattg cttgctacta
    12961 attgccctgc atcaaaaatc aataagcaat tgctacccgg ccaaaacagt agtataagta
    13021 aatttgcttt cagggtttta caagaggcgt gttgtgcata gttctgaatt tgctaacatg
    13081 atttttaaaa atatcatcat ctatataata aaaagattgt ggaggataaa aattatcaaa
    13141 aatatcattg gggtttaatg gtgttcaaag atttcaagat gtccaatttt aatggcatag
    13201 ccattttttt accggaaaaa tagctaaaaa agtctgtttc atcgatacct gcatatttat
    13261 taaacttttc ccatagttta gatggatggc cttgaatgat ttcttctata aaaaaatatc
    13321 caataatttg cttatctgga aaagttgaat aaatataagc tttatcagta ttttctttga
    13381 aaattgtttt ttaaaattca aattttttat taccttttat tatttgatta acgaattttg
    13441 gtttaatcga aagtaaaacc atgatcaata tccccctgtc ttttatcgtt aagtaacttc
    13501 catggcctat ttcagttatt gactgtggag g
//
LOCUS       DAOOJH010000064        13311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4362_length_13311_cov_9.882770, whole genome shotgun sequence.
ACCESSION   DAOOJH010000064 DAOOJH010000000
VERSION     DAOOJH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4362_length_13311_cov_9.882770"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..>1434
                     /locus_tag="PLJ75_08770"
     CDS             424..>1434
                     /locus_tag="PLJ75_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05344.1"
                     /translation="MFIFEWLSKKISYTFAVCFFTIFGGWMSAVFGYYLGTSFILSDY
                     NEMNALALKWLPLSVLIPTLLHYISFGLLTPIKIPAFLKPLRDINKAFNNGTLNPTLD
                     DETLKNIYVQLSHLPLYNMIAAALFGAMCGFFLMGFGYYDMYTSGTLTIVKMKTGIKA
                     VTIGVLVVIILYAMSTYLLTEIISNQHRAFVYQELRQRNINIYPRGLIGLRIKFSFFI
                     ILMIITLLTFAAIMEQYRLYEGAQYVNIITYMVISIIAGIFVMYINSDSVMRVLVEMG
                     VVTKNIASGQNAWFKVMSHEEEFAEIEYSILEMATQIEEHRKNLELKVEQRTEELREA
                     LAS"
     assembly_gap    1435..1534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1535..2458
                     /locus_tag="PLJ75_08775"
     CDS             <1535..2458
                     /locus_tag="PLJ75_08775"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD05345.1"
                     /translation="PKRVFQEVNQNILEHIKTQDYMTAFFVVIDDDYNIVYANASHQK
                     AILVHSDEGRVEFLDTNGLFIGAIEEARETYEEKVTKLRYGDRLLLYTDGIPEAANVD
                     REEYSIERLQDAALKNRHLPLEDFTSFIIEDVQRFKGKAPVEDDITLLVIELARDEAV
                     DIIKQSKKLIDEHKYYEAIDYLERSLTLYPNNRKLIYNLAKNYFRVNNFGKASELIEQ
                     YLEMDKRNKYAYYVGGAAYYQMMNYEKAIQLLERAQSIDPNFTNALFALGMAYKKIGM
                     SQDALQIFERVANIDPDNKMALFEINEIRKK"
     gene            2519..4399
                     /locus_tag="PLJ75_08780"
     CDS             2519..4399
                     /locus_tag="PLJ75_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD05346.1"
                     /translation="MLLKNRNKKSSKKSNPIRKVYFGLRLRLILVLSVVLLIVLLIFT
                     AFMIYNQTIIMSQEKNEKATILAQNLSNIFEWYLDKSKYETIEDSKQKLKLIKEEITK
                     FKNNNQDILYIFLYDYYNTIAYKSTQIDITKEPYYKVVKSYLTDRQQKLITHDYTAGK
                     DISKNNKFYKNKQYKVVLCPVIAKTGILMQINEDFDTILLKSHTQSFTNDEKKKFYTK
                     LWVKYHDLLPDKFDPKNFDKKNENLIDCNLDIIFLQLYKNIFSIKKNVVEKKDLWLLN
                     EKWILHNYTIISRGILNKDIALIKKYDDIIIDNLKVLRDYGEKFKHLGTLIVVYDLNQ
                     IQSDIYKSLSNGYITALSIYIISIMVIFWITGIMLKNIKKLEKWALDVGKGNFSLPID
                     IKNNDEIGRLSDVFQYMINEINSKYHLEKFVSRSTRHLIGMAGFEKIDVGFFDRRNFA
                     FLFSDIRGFTSFSENNDPETVMSVLNLYFDKQAKIIQKYKGDIDDFVGDQIMAHFGGT
                     KKADTAITVAIGIMKEIDLLNKERKSMNLPIFEIGIGIHVGDVVIGKIGAEFRMDLAC
                     IGDVVNTTSRLCQNAQAGEIIVSKSIISEITKTFKYKKLEPVKLKGKKDPLEVYSVKW
                     ES"
     gene            complement(4429..5028)
                     /locus_tag="PLJ75_08785"
     CDS             complement(4429..5028)
                     /locus_tag="PLJ75_08785"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPD05347.1"
                     /translation="MFTIVVISEIHGQIKSKQRDENTVEYYNIGNKKEKGNNYQQTNI
                     LKNPYDSLIVTIANKENIDPHLIRCIIKIESNFNKDAVSVAGAMGLMQLMQDIVQAYN
                     VDDPFNPEQNIKAGVKHFKSLLALLNNDIVLALAAYHAGIGRVKKNMTVPNISSTIEY
                     VNAVMKLYKPQENNNYTTKIEKLYLQILPDGTINITTIK"
     gene            complement(5072..6517)
                     /locus_tag="PLJ75_08790"
     CDS             complement(5072..6517)
                     /locus_tag="PLJ75_08790"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPD05348.1"
                     /translation="MNITFITSEAYPFAKTGGLADYSYSLPKALAKLGHTVNLFLPRY
                     YCVDKIKYNCSLIDMPLAVPHDDGIRWAGIYKSNYLDTINVYFIEHDVFFGRDGLYDY
                     HGIPYGDNAERFIYFNRATLEALKQLAIVTDIIHCNDWQTALIPLFLKTHYAHESIFK
                     NTKSVLTMHNAGYQGVFPKDVLYYMQLSDEYFVPERLAFYDKINFLKAGILYADCLTT
                     VSKRYAQEIQYPDYGYDLAPIIKQNSSKLFGILNGVDYEQWNPEYDKFIPCNFSINDV
                     SGKKKCKYALQDYFSLNKSDIPLLGCISRLTYQKGIDILIDALDWLLFDEPEVQFVLL
                     GNGEQWLIDRFENLRKLYPDKVGVWWGYNEKLSHCIEAGCDFYVMPSRYEPSGLNQMY
                     SMAYGTIPIVRATGGLDDSVIEFNTTELTGNGFKYKHNDIAELYSTIRYALKIYNNSN
                     LMQQLIYNAMSFKRSWDDAAKEYEALYKNIK"
     gene            complement(6519..8171)
                     /gene="gpmI"
                     /locus_tag="PLJ75_08795"
     CDS             complement(6519..8171)
                     /gene="gpmI"
                     /locus_tag="PLJ75_08795"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002702138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPD05349.1"
                     /translation="MKLAPNGFKRRKGALVLIIMDGVGAGTNDDGNAFFLARTPVFDS
                     LSKSCPYTLLKAHGTAVGLPSDADMGNSEVGHNALGAGRIFDQGAKLVDKSIESKQIF
                     TTDVWKKLIDKPVKSKSTLHFIGLLSDGNVHSHINHLFKLIEQAAHEGVKKIRVHILL
                     DGRDVPETSALEYVSSLENFLASFNARGFDFCIASGGGRMVTTMDRYEADWNIVKRGW
                     DAHVLGKGRAFTSATEAIETFRKEQPDITDQYLPAFVITKNGKPAGTIQDGDSVILFN
                     FRGDRAIEISRAFEDKNFNKFDRERYPDVVFAGMMEYDGDLHIPKNYLVFPPSIDKTV
                     SEYLVHNGCKQFAISETQKFGHVTYFWNGNRSGMFDPQYEVYQEIPSDRVQFDQRPWM
                     KAAEITDAVIEALNSNKYDFIRLNFPNGDMVGHTGNLEAAIIAAEVVDLCIGRLLKAI
                     NDTQALAIITADHGNLDEMFEKDTKTGAIIIDKKTGKPQPKTSHTLNPVPFIIYDPHF
                     KGEYCLTPQTNQGLANVAATICTLLGYHPPEEYYPSLITLAQ"
     gene            complement(8172..8474)
                     /locus_tag="PLJ75_08800"
     CDS             complement(8172..8474)
                     /locus_tag="PLJ75_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05350.1"
                     /translation="MIFTKREEDNRCIFIINEQLNPRTLKELIEEMHNFLQNDSRDAI
                     IDMSNVEIVDSTILAGFMTLYNNFKNNHKKFRLINTNSYVKRIIELASLDTFLLEE"
     gene            complement(8483..8827)
                     /locus_tag="PLJ75_08805"
     CDS             complement(8483..8827)
                     /locus_tag="PLJ75_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05351.1"
                     /translation="MNFTSNRYDDIVIINIAGIGEYGDDVKLIKYMEKLLDNKLYIVA
                     VNFSDVDTINSAAVAALVYLLKYGDEHGFDVAFFGANDKIIMILERALPKYAINIFTE
                     EEFYKRFNIPNK"
     gene            complement(8824..9528)
                     /locus_tag="PLJ75_08810"
     CDS             complement(8824..9528)
                     /locus_tag="PLJ75_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05352.1"
                     /translation="MSYHVNKNILNCNTLLIAVIITLSFIFLLPQYLYALAIVDTYHQ
                     IQTTFEGLSKFLNSIYTVVSTITSILGYRAIVLFFAIAVTSAGLSAIGFPKGKTTFFI
                     AFIIDNILWYTWNKSFGIDYTATVLTMLKTNAVIVFPYIIFIIIKRYALQIKHFFIQI
                     FHMVLPSHYKKKITKNALSETINSLHSAYATMMSSIMNDLIHSNDTINLSHDTIVTIE
                     HIESILKKIKETGDKQ"
     gene            9964..10530
                     /locus_tag="PLJ75_08815"
     CDS             9964..10530
                     /locus_tag="PLJ75_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPD05353.1"
                     /translation="MDELIENIGKRIQYYRKKNDTTLKELADKIKATPSLISQIEHGK
                     ANPSLGTLKAIADVFGVPIGLFFENDIKRTTSSPVIRKNTHRKLITNGNVAYTLLNPD
                     SEDMEVILIEFPPQVATGEEHYQHDGYEVGYVIKGELTVVLDDNEYNLQLGDSIAFRS
                     IRPHTIKNKSSQNAVAVWVNLIPWIFVK"
     gene            10532..11686
                     /locus_tag="PLJ75_08820"
     CDS             10532..11686
                     /locus_tag="PLJ75_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPD05354.1"
                     /translation="MQLLKGAIATMNDETNTTIDTQSTKYTQLKIKLSVVHMVLELLL
                     LGIGAFTISKPIYRFVATHIHNEYVQLIAFFITFGGLLSLMLLPLEFYQSYVVEHKFK
                     LSNQTFLRWCSEELKSLMVSLIIGVPLLLLFYFFIKLTSNWWLYFAIVVFIVAIVLAK
                     VAPVLIFPLFYKFTPIDNEQLKNTLNDLMQEHGLRLKGIYSFNMSKNTNKANAAFTGL
                     GKSRRIILSDTLINEFSINEIKSVFAHELGHYIHKHILKNIIISGFIIIISFYICSYF
                     YEITLPSAGYTNRFDIAALPLLIFYLSVFSLLLMPVLNTLSRYYEKQADMYALATTND
                     PDSFISTMEKLAAMNLSTKEQHPLIEFFMYSHPTIAKRIALANDYRRIQS"
     gene            complement(11654..12679)
                     /gene="purM"
                     /locus_tag="PLJ75_08825"
     CDS             complement(11654..12679)
                     /gene="purM"
                     /locus_tag="PLJ75_08825"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPD05355.1"
                     /translation="MTYKDSGVDVEGGNKFVKRIRSLVKDTFTKEVLTDIGGFAALFD
                     ASFKQYKHPVLVSGTDGVGTKLKIAQMMNIHNTIGIDAVAMCVNDIIVTGARPLFFLD
                     YISCGKLHEDVLVNIIQGIASGCKMADCALIGGETAEHPGIMQPDDYDIAGFSVGVVD
                     KQNSINGNTINPGDIIIGIASSGVHSNGYSLVRKLFFDIKKYTIETKFHELSKPLGAT
                     LLEPTKIYVKPILKALESIAIKGMVHITGGGFYENIPRILPKNTAAYIDKSSFPIPPI
                     FSIIQHEGNIDEREMFTTFNMGIGFICIVSKKDADALITGLTRHGEKAYSIGEIKSRL
                     DSPVIIS"
BASE COUNT         4511 a         2071 c         2176 g         4453 t
ORIGIN      
        1 acaggcaaat tttccaatca tattaaatta aaaaatactt gctatttttt ataatgtacg
       61 ggaacctatt ataagtatat tattgatgat atttttttga attgcaatat agcattatac
      121 ctatatagta taaaaaccct aaaagatgct gtcatagtgc ggtatgctgg gtggcaaatg
      181 gttttgtaaa atttttttta gtttatagag gtagcagaat tacggtagta tatgttccat
      241 tattataatt attaattata ccgattagta cagtaagttg tatttttctt ttatttcaat
      301 gagtattgta taaaaatgta taaatactat gatagtaaat ttgatgaggt ttatgctatg
      361 ggtacggctt tatactattt aatttagaaa taattctgtt ttttacagga gatgacataa
      421 attatgttta tatttgaatg gctgagtaaa aaaatatcat atacctttgc tgtttgtttt
      481 tttaccattt ttggcggatg gatgagcgca gtgtttggtt attatcttgg cacatcattt
      541 atcctttctg attataatga gatgaatgca ttagcgctta aatggctgcc actatctgta
      601 ttaataccaa cgttgttaca ttatatatca tttggtttgc tcactcccat taaaataccg
      661 gcatttctta agccacttcg cgatattaac aaagcattta acaatggcac gttaaatcca
      721 acgcttgatg atgaaacttt gaagaatatt tatgtccagc tttcacattt accattatat
      781 aatatgatcg ctgcagcact atttggggca atgtgtggtt tttttcttat gggttttgga
      841 tattacgata tgtatacttc gggaacatta acgattgtaa aaatgaaaac cggaataaag
      901 gcagtaacta ttggtgtact ggtagtcatt attttgtatg ccatgtctac atacctgtta
      961 accgagatta ttagcaacca gcatcgggct tttgtatatc aggagttgcg gcaaaggaac
     1021 atcaatatct atccaagagg attgattggg cttagaatta agtttagttt ttttatcata
     1081 ctaatgatta ttacactgtt aacatttgct gcaataatgg agcaatatcg tttgtatgaa
     1141 ggagcacagt atgttaatat cattacatac atggttattt caataatagc aggtattttt
     1201 gtaatgtata ttaattctga ctcggttatg cgggtacttg tagaaatggg tgttgttaca
     1261 aaaaatattg cttcagggca aaacgcatgg tttaaggtaa tgtcacatga agaagaattt
     1321 gctgagattg aatattccat attagaaatg gcaacgcaaa tcgaagaaca tagaaaaaat
     1381 ctggaattaa aagttgaaca gcgtactgaa gaattgcgcg aagcattagc aagcnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncctaaa cgtgttttcc aggaagttaa
     1561 tcagaatatt cttgaacata taaaaacaca ggattatatg acagcatttt ttgttgttat
     1621 tgatgatgat tacaatattg tttatgcaaa tgcaagccac cagaaagcaa ttttagtaca
     1681 ttcggatgag ggtagagttg aatttctgga taccaatggt ttatttattg gtgctattga
     1741 agaagcaaga gaaacatatg aagaaaaagt tacaaaattg agatacggtg acagattgct
     1801 tttatatact gacggcatac ctgaagctgc aaatgttgac cgtgaagaat attcaattga
     1861 aagattgcag gatgctgctt tgaaaaaccg gcatcttcca ttagaagatt ttaccagctt
     1921 tattattgaa gatgtgcagc gatttaaggg aaaggctcct gttgaagatg atattacgtt
     1981 acttgttatt gaactggcaa gggatgaagc tgttgacatt ataaaacaat caaagaagtt
     2041 gatagatgaa cacaaatact atgaagcaat tgattatctt gaaaggagtt taacgctata
     2101 tccaaataat cgtaagctta tatacaattt agcaaaaaat tatttccgtg ttaataattt
     2161 tggtaaagca tcagaactta ttgaacaata tttagaaatg gataaacgta ataagtatgc
     2221 ttactatgtt gggggcgcag catattatca aatgatgaac tatgaaaagg caattcaatt
     2281 gctggaaaga gcacagagca ttgatcctaa tttcaccaac gctttatttg ctttaggtat
     2341 ggcatacaaa aaaataggaa tgtctcagga tgcattacag atatttgagc gtgtagctaa
     2401 catagatcct gataataaga tggcattatt tgaaataaat gaaataagaa aaaaatgata
     2461 aaaggcaaaa catttttatc atttcaatat tattatattg aataaacgaa tatctactat
     2521 gcttttaaaa aatagaaata aaaaatcttc aaaaaaaagt aacccgatca gaaaagtata
     2581 ttttggtctt agattgcgtt taatattagt actaagtgtt gttttgctca tagtattatt
     2641 aatatttact gcatttatga tttataatca aactattatt atgagccagg aaaaaaatga
     2701 aaaggctaca attttagcgc aaaacctgag taatatattt gaatggtatt tagataaaag
     2761 caaatatgaa acaatagagg attcaaaaca aaagttaaaa ttaataaaag aagaaataac
     2821 aaaatttaaa aacaacaatc aagatatatt atatatattt ctttatgatt attataatac
     2881 aatagcatac aaatcaacgc aaattgacat tactaaagaa ccatattata aagttgtaaa
     2941 aagttattta acagatcgtc agcaaaaatt aattacgcat gactatactg ctggaaagga
     3001 tatatcaaaa aataataaat tttataaaaa taaacagtat aaagttgttc tatgcccggt
     3061 aatagcaaaa actggcatat tgatgcaaat taatgaagat ttcgatacta tacttttaaa
     3121 aagtcatact caaagtttta caaatgatga aaaaaagaaa ttttatacta aattgtgggt
     3181 taaatatcat gatttattgc ctgacaaatt tgacccaaaa aattttgata aaaaaaatga
     3241 aaatttaata gattgcaatc ttgatataat tttcctccag ctatataaaa atattttttc
     3301 tatcaaaaaa aatgtagtag agaaaaaaga tttatggtta ctcaatgaaa aatggatatt
     3361 acacaactat acgattattt ccagaggcat tttaaataaa gacattgctc ttattaaaaa
     3421 atatgatgac attataatag ataatttaaa ggttttacga gattatggag aaaaatttaa
     3481 gcatttaggt actttgattg ttgtatatga tttgaatcaa atccagtcgg atatctataa
     3541 aagcctttcc aatggatata taacagcatt atcaatatat attataagta taatggttat
     3601 tttctggatt acagggataa tgttaaaaaa tattaaaaag ttagaaaaat gggctttaga
     3661 cgtaggcaag ggaaatttca gtttgcctat tgatattaaa aataatgatg aaattggaag
     3721 attatcagat gttttccagt atatgataaa tgaaataaat tcaaaatatc atctggaaaa
     3781 gtttgtttca agatcaacta gacacctgat tggaatggca ggatttgaaa aaattgatgt
     3841 aggttttttt gatagaagaa attttgcgtt tttgtttagt gatatcagag ggttcacaag
     3901 tttttctgaa aataatgatc ctgaaactgt tatgagtgtt ttgaatttat attttgataa
     3961 acaggcaaag attatacaaa aatataaagg ggatatagat gattttgtag gtgatcagat
     4021 aatggcgcat tttggcggta ccaaaaaagc tgatacagca attacagttg caattggaat
     4081 tatgaaagaa attgatttac taaataaaga aagaaaatca atgaatttac ctatttttga
     4141 aataggtata ggtatacacg taggtgatgt cgttataggt aaaattggtg cagaatttag
     4201 aatggatctt gcatgcatag gtgatgtagt aaatacaaca tcacgattat gccaaaatgc
     4261 acaggctggc gaaataattg tatcaaaaag tattataagt gaaataacaa aaacatttaa
     4321 atataaaaag ttggaacccg ttaaacttaa aggtaaaaaa gatccattag aagtatattc
     4381 tgttaaatgg gaatcataat ttaccgtatc aacatggttt ttaataaatc atttaatggt
     4441 tgttatgttg attgtgccat caggtaaaat ttgaaggtat agcttttcta tctttgtggt
     4501 ataattgttg ttttcttgtg gtttatataa cttcataact gcattaacat attcaattgt
     4561 tgaactaata tttggcacag tcatgttttt tttcactcga cctatgcctg catgataggc
     4621 ggccaacgct aacacaatat cattattcaa taatgctaaa agagatttaa aatgctttac
     4681 tcctgcttta atattttgtt caggattaaa ggggtcatca acattatatg cctgcactat
     4741 atcctgcata agctgcatca aacccatcgc accggcaact gaaacggcat ctttattaaa
     4801 atttgattca atctttataa tgcatctaat taaatgagga tcaatatttt ccttattggc
     4861 gatagttact attaatgaat catatggatt tttaagaata tttgtttgct gataattatt
     4921 tcccttttcc tttttattcc cgatgttata gtattctaca gtattttcat ctctctgttt
     4981 ggatttaatc tgcccatgaa tctctgatat gacaacaata gtaaacatta atatcattgc
     5041 aattatcaat gtatgatgag caaaatgtat attacttaat atttttatac aatgcttcgt
     5101 attcttttgc tgcatcatcc cagctccgtt taaaagacat cgcattatat attaattgct
     5161 gcattaaatt gctattgtta taaatcttta atgcataccg aatggttgaa tataattcag
     5221 caatatcgtt atgtttgtat ttaaatccat ttcccgtgag ctctgttgta ttaaattcaa
     5281 tgactgaatc atcaagccca cccgttgccc ttacaatggg aatggtaccg tacgccatgc
     5341 tatacatctg gtttaaacct gaaggctcat agcgtgatgg cataacataa aaatcgcatc
     5401 cagcttcaat acaatgagat aatttttcat tgtaccccca ccaaacaccg accttatcag
     5461 gataaagctt ccgaaggttt tcaaatctgt caataagcca ttgctcccca ttccctaata
     5521 gtacaaattg cacctctggt tcatcaaaca aaagccaatc caatgcatca atcagtatat
     5581 caataccttt ttgatatgtc aacctgctaa tacaccctaa caatggaata tcacttttgt
     5641 ttaatgaaaa gtaatcctgc aaagcatatt tgcacttttt tttgcctgat acatcgttga
     5701 ttgaaaagtt acagggtata aatttatcat actcaggatt ccattgctca taatcaacac
     5761 cgttgagaat gccaaaaagt tttgagctgt tttgtttaat aattggtgcc agatcataac
     5821 catagtcagg gtattgtatc tcctgagcat agcgtttact gactgttgtt aaacaatctg
     5881 catatagaat gccagcttta agaaagttta ttttatcata aaatgccaat cgttctggaa
     5941 caaaatattc gtcagatagt tgcatataat ataatacatc ttttgggaaa acaccctgat
     6001 atccagcgtt atgcatggtt aaaacgcttt tagtattttt aaatatagat tcatgagcat
     6061 agtgggtttt taaaaataga ggtatcaatg ctgtctgcca gtcattgcaa tgaatgatat
     6121 cggtaactat cgccaattgc tttaacgcct caagggtagc acggttaaaa tatataaacc
     6181 gctcggcatt atcaccgtac gggataccgt gataatcata taatccatca cgaccaaaaa
     6241 aaacatcatg ctcaatgaag taaacattga tagtatcaag ataattagat ttatatatac
     6301 cagcccagcg aataccatcg tcatgaggta ctgctaacgg catatcaatg agtgagcagt
     6361 tgtattttat tttatcgaca caataatagc gtggtaaaaa aaggttaacc gtatggccca
     6421 gttttgccag cgcttttggc aatgagtatg aataatcagc aagaccacct gtctttgcaa
     6481 atggataggc ttcacttgtt ataaatgtta tattcatatc attgtgcaag ggtaatcaat
     6541 gagggataat attcttcagg tggatgatat ccaagcaatg tgcatatagt agcggctaca
     6601 tttgcaagtc cctgattggt ttgcggcgta aggcaatatt cgcccttaaa atgggggtca
     6661 tagattataa acgggacggg atttaacgtg tgtgaagttt ttggttgcgg cttcccggtc
     6721 ttcttatcaa tgataatggc tcctgttttt gtatcttttt caaacatttc atctaaattg
     6781 ccatgatccg cagttattat agctaatgcc tgtgtatcat taattgcttt taataaacgt
     6841 ccaatgcaca gatcaacaac ctctgcggca atgattgcag cctctaaatt accagtatgt
     6901 cctaccatat cgccattggg aaaatttaag cgaataaaat cgtatttgtt gctatttagt
     6961 gcttcaataa ctgcatcagt aatttcagca gctttcatcc atggacgctg atcaaattgc
     7021 accctgtcag aggggatttc ttgatacact tcgtactgtg ggtcaaacat cccgctacgg
     7081 ttaccattcc agaaataggt aacatggcca aatttttgtg tttctgaaat ggcaaattgc
     7141 ttacagccat tatgaacaag atattcactg acggttttat caattgacgg aggaaaaaca
     7201 agataatttt ttggtatatg taaatctcca tcatattcca tcatccctgc aaacacaaca
     7261 tcgggatatc gttcacgatc aaatttatta aaatttttat cctcaaaagc ccgtgaaatc
     7321 tcaattgccc ggtcacctct aaaattaaat agtataaccg aatcgccatc ctggatagtt
     7381 ccagcaggct tgccgttttt tgtaatgaca aaagcaggta agtactgatc ggtaatatcg
     7441 ggctgttctt ttctaaatgt ttcaatagct tctgttgcag atgtaaatgc tcgccctttg
     7501 ccaagaacat gagcatccca cccacgcttt acaatattcc agtctgcttc atatctatcc
     7561 atggtggtta ccatcctgcc tccaccagag gcaatgcaga aatcaaatcc tcgtgcatta
     7621 aatgatgcca aaaaattttc aagactgcta acatattcca gtgcagaagt ttcagggaca
     7681 tcacggccat caagaagtat atgaacgcga atctttttta ctccttcatg agctgcctgt
     7741 tcaatgagtt taaacagatg gtttatatga gaatggacat taccgtcaga aagtaatcct
     7801 ataaaatgaa gtgttgattt agattttact ggcttatcaa taagtttttt ccatacatca
     7861 gttgtaaaaa tttgttttga ctctatggat ttatcaacca gttttgcacc ctgatcaaaa
     7921 atccttcctg ctcccaatgc attgtgccca acctctgagt ttcccatatc agcatccgat
     7981 ggtagcccaa ctgccgtacc atgtgctttg agcagggtat agggacagct tttgctcaat
     8041 gaatcgaaaa caggtgttcg tgctaaaaaa aaagcattgc cgtcatcgtt agtaccggca
     8101 ccaactccat ccataataat caatacaagg gctccttttc tacgtttaaa accgttgggt
     8161 gctaatttca tctactcctc cagcagaaaa gtatcaagtg atgccaattc aataatcctc
     8221 ttcacataac tatttgtgtt aattaatcta aactttttat gattattttt aaagttatta
     8281 tataaagtca taaacccagc cagtatagtg gaatctacaa tttcaacatt tgacatatca
     8341 attatagcat ccctgctgtc attttgtaaa aaattgtgca tttcttcaat caattccttt
     8401 aaggttcgcg gattcaattg ttcattgatt ataaaaatac aacgattatc ttcttccctt
     8461 tttgtaaaaa tcatggctca cctcatttgt ttggaatatt gaaacgttta taaaactctt
     8521 cttcagtaaa aatatttatt gcatacttag gtagtgcacg ctccagaatc attataattt
     8581 tatcatttgc gccaaaaaaa gcaacatcaa aaccatgttc atcaccatat tttaaaagat
     8641 aaaccagtgc agcaacagca gctgaattta tagtatcaac atctgaaaaa tttactgcta
     8701 ctatatataa tttattatca agtaattttt ccatatactt aattaatttt acatcatcac
     8761 cgtattcgcc aatgccagca atattaataa taacaatatc atcataacga ttggatgtaa
     8821 aattcactgt ttatctccgg tttctttaat ttttttaagg atagattcta tatgttcgat
     8881 agttactatg gtatcatgtg aaagattaat agtatcgttg ctatgaataa gatcattcat
     8941 gattgacgac atcattgttg catacgcgct atgtaatgaa ttgattgttt cagacagagc
     9001 attttttgtt attttctttt tataatgaga tggcaatacc atatgaaaaa tctgtataaa
     9061 aaagtgcttt atctgcaatg catatcgttt tataataata aatataatat acggaaatac
     9121 tattacagca tttgttttaa gcattgtcag cactgtagca gtataatcta tgccaaagct
     9181 tttattccat gtataccata atatattatc tattataaat gcaataaaga atgttgtttt
     9241 ccctttggga aatcctattg cagaaagccc tgctgatgtt actgcaatgg caaaaaagag
     9301 tacaatggca cggtatccca aaattgatgt tatggtagat actacagtat atatactgtt
     9361 taaaaacttt gataatcctt caaatgtagt ttgaatctgg tgatatgtat caacgattgc
     9421 aagggcataa aggtactgag gtaataaaaa aataaaggat aaagtaataa taactgcaat
     9481 cagtaatgtg ttgcaattca gaatattttt atttacatga taactcattg cactatactc
     9541 tgtactataa ttatttaaag taatacccgt cacattgaat aatgctttct catataatac
     9601 ataatataaa aagcatattt attactatcg tattcaaata tgggtgtaaa aacaaaagtt
     9661 tcagttgtat cattatttat aatgatattt ttttgctcca ttttaactgc aattataatc
     9721 tcaaaactgc tttatatcag gaaacacagt aactatcaat atttaaatta cttttttaca
     9781 aaaaataata ttttatccat tttaatttaa tcttttttaa attgtcaaat attttaacat
     9841 aaatgatcca ataaattgta atcaagaatt tataaaaatt aatagacaaa ataactaatt
     9901 taaattatat ttaaatcact ggatataaaa cgctggtatt acatttataa aggaaaactt
     9961 atagtggatg agctcataga aaatattggt aaacgaatac aatattatcg aaaaaagaat
    10021 gatacaacat taaaagaatt agctgataaa ataaaagcta ctccaagcct cataagtcag
    10081 atagaacatg ggaaagctaa tccatcactt ggaacactca aagcaatagc tgatgtgttt
    10141 ggcgtaccca taggattgtt ttttgaaaat gatattaaac gtacaacatc ttcaccagtt
    10201 atacgtaaaa atacccatcg taaattaatt acaaatggaa atgtagcata tacattgtta
    10261 aatcctgatt cagaagatat ggaagtaata ttgattgagt tcccaccaca agtggctacc
    10321 ggagaagagc actatcaaca tgatggctat gaggtaggtt atgttataaa gggcgagctt
    10381 accgttgttc tggatgacaa tgaatataat ctgcaattgg gtgatagtat cgcttttaga
    10441 agcatcagac ctcatacaat aaaaaataaa tcatcgcaaa atgcagtagc agtatgggtt
    10501 aacttaattc catggatttt tgttaaataa tgtgcaattg ttaaaaggag ctatcgcaac
    10561 catgaatgat gaaaccaata ccacaattga tacacaatca acaaagtata ctcaattaaa
    10621 aataaagctt tcggttgttc atatggttct ggaacttttg ttgctgggca ttggtgcatt
    10681 tactatttct aaaccaatat ataggtttgt tgctacgcat atacacaatg aatatgtaca
    10741 gttgattgct ttttttataa catttggcgg cttgctgtca ttgatgcttt taccgcttga
    10801 gttttatcaa agctatgttg ttgaacacaa atttaaactt tccaatcaaa cttttttgcg
    10861 gtggtgcagt gaagaattga aatcattgat ggtttcattg attattggcg tgccattatt
    10921 attgctattc tactttttta taaaattaac atcaaactgg tggttatatt ttgcgatagt
    10981 tgtttttata gttgcgatag ttctggcaaa ggtggcaccg gttttgatat tcccgttatt
    11041 ttataaattt acaccaatag ataatgaaca gttaaaaaat acattgaatg atttaatgca
    11101 ggaacacggg ttgagattaa aaggaatcta tagctttaat atgagcaaaa atacaaacaa
    11161 ggcaaatgca gcttttacgg gattgggtaa aagccgaaga attattctga gtgatacatt
    11221 gattaatgaa ttttcaatca atgaaatcaa atcagtattt gcccacgaat taggacatta
    11281 tattcataaa cacatactta aaaatataat tatcagcggt tttatcatta ttatatcatt
    11341 ttatatatgt tcatattttt atgaaattac cttgcccagt gcgggatata caaacagatt
    11401 tgatattgca gcgttgcctt tattaatttt ttatctttca gtatttagct tgttgttaat
    11461 gccagtattg aatacactat cgcggtacta tgaaaagcaa gccgatatgt atgcacttgc
    11521 aacaaccaat gatcctgatt catttatttc aactatggaa aaattagctg caatgaatct
    11581 gtcaacaaag gagcagcatc cgctcattga attttttatg tatagccatc ctacaattgc
    11641 aaaacgtatt gcgttagcta atgattacag gagaatccag tcgtgatttt atttcgccaa
    11701 tactatatgc tttttcacca tgacgggtta aacctgtaat aagcgcatcg gcatcttttt
    11761 ttgacacaat acaaataaag ccaataccca tattaaacgt tgtaaacatc tctctttcat
    11821 caatattacc ttcatgctgt ataattgaga atattggagg aataggaaat gaacttttat
    11881 caatataagc agcagtgttt ttgggaagta tacgggggat attttcgtaa aatccaccgc
    11941 cggtaatatg taccatgcct ttgatagcta ttgattcaag cgctttgagg ataggtttaa
    12001 cataaatttt agttggttca agcagagttg cgcctaatgg cttagatagt tcatgaaatt
    12061 ttgtttcaat ggtatacttt ttaatatcaa agaacaattt tcgcacaagc gaatagccat
    12121 tagaatgaac accagatgaa gctatgccta taatgatatc acctggatta attgtatttc
    12181 catttatgct attctgtttg tcaacaacac caacgctgaa tccagctata tcataatcat
    12241 caggttgcat tatgcccgga tgttctgctg tttcgccgcc tattaaggca caatcagcca
    12301 ttttgcatcc tgaagcaatg ccctgtatga tattgacaag tacatcttca tgtagttttc
    12361 cacatgaaat ataatctaaa aaaaataaag gtctggcacc tgttacaata atatcgttga
    12421 cacacattgc tactgcatca atgccaatgg tattgtgaat attcatcatc tgggcaattt
    12481 ttaactttgt gcctacaccg tcagtgccgg aaacaagtac cggatgttta tactgtttaa
    12541 atgacgcatc aaaaagggct gcaaaaccac caatatcagt caatacctct tttgtaaatg
    12601 tgtcctttac cagtgaacgt attcgtttaa caaatttgtt gccgccttca acgtcaacac
    12661 cagaatcttt ataggttatt cccatggtta cctcttgagt ttatttgctt tttatcttcc
    12721 agggttatct tgtgacatga tgcctattgt atgtcaatta attattcgat gtttctgtgg
    12781 aaatcttatt tattaatggt ataccaattg cttttcatgt gcatctttaa acgatttgca
    12841 ttttccaggt ttcccatcaa tttagcgttc gatagctgcc tgaaaaagtt tcttaactct
    12901 tatcccgcaa aattctgttc agagacaaat catctaatag attttatgct tgactatttt
    12961 gttatattca cgttatcttt tatttcatgg tgacaaatcc aacgtattta aattgttttg
    13021 cttaaacttt tttatctgag tatatattac tttttatgtc atccaccaaa ttatagcata
    13081 attattagga aatataacga gttctttaga tacattaaac ctcaagaaag gaggtgggtg
    13141 gaaacttttg aaagataaaa agaatcaggg aaattcagcc agaatcagta tagacgaaat
    13201 acttcgtata tactcacaaa acaggataaa aagcgaagta gttcatatat gaatgagtta
    13261 tatgctattt gctcgccaac tttatggggg cgccgccatc cgtggcggct t
//
LOCUS       DAOOJH010000065        13214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4398_length_13214_cov_9.448970, whole genome shotgun sequence.
ACCESSION   DAOOJH010000065 DAOOJH010000000
VERSION     DAOOJH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4398_length_13214_cov_9.448970"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLJ75_08830"
     CDS             <1..534
                     /locus_tag="PLJ75_08830"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPD05356.1"
                     /translation="PLDATNNFAHKIPWFCVTIALYSKALEAAVAGVVYEPLRDECFY
                     AWKDGGAYVNEKRIFVSQINDIGISIIATGFPYKKDDPSCNNAKQFNNVLPYVQGMRR
                     MGSAAIDLSYVACGRFDGYWEPLLYPWDMTAGALLVEEAGGMVTQYNGNTFDPEFPEI
                     CATNGLIHQQLLELINK"
     gene            658..1248
                     /locus_tag="PLJ75_08835"
     CDS             658..1248
                     /locus_tag="PLJ75_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05357.1"
                     /translation="MTKKIMCLCFVLVASTLLAQNTQSVDIPVQSLYSNKVRIKDVYF
                     NKKVDLTGKGEVLEVEAILENLTDDPIDVYVNIIATYEKEEKTKSSFEMPIPEKERVR
                     NFVPFPDDIANYQYPDGKGGNKLKAYPKNTKSGIDPLTGKLYHLPVNDIVLVRSTNLA
                     PYRKDFLFFNYVTILVFDNNGTPILREVFELKGKRK"
     gene            complement(1324..>1827)
                     /locus_tag="PLJ75_08840"
     CDS             complement(1324..>1827)
                     /locus_tag="PLJ75_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPD05358.1"
                     /translation="YDKNLINKIEQQYNISEKIVETIDTRRREEMSELWRTMLADLPP
                     QAAVFQKLANTIRTIAIHGNAILIGRGSVIITKGLRYGLHLRFVAPLQYRIQKVMEYQ
                     KIKDRLAAEKLIEEKDRQRHEFMTQYIKFNAYDPASYDITINVAQFTKEQVAQIGIAG
                     LKVRKLI"
     assembly_gap    1828..1927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1928..4299
                     /locus_tag="PLJ75_08845"
     CDS             <1928..4299
                     /locus_tag="PLJ75_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD05359.1"
                     /translation="YLLQQHGISDITMSLFYLPENVVNYFGDGTFWDVAIDYSIEEIP
                     LGTAGGVKLALKDYDDTFIVLSGDGLYDFNVHDIIQFHKEKRSLFTIALAHVPDPVEY
                     GIVVTHEDGTIDRFLEKPSWGEVFSDTVNTGLYIIEPEIIDKFVPDNGKFDFSYDLFP
                     LLQKKGIPLYGYVTDGYWCDIGNLDAYRKVHQDILNGIVKINFPGKKIGENIWAGDDV
                     EIHADAILKGPIVLGNFVNIKKGAEVSEYSVIGDNTVIHENASVRRSVILHNTIVGTK
                     TEIRGAVVGKRNVIEDNVSIYEGAVISDDCQIGNGAKIATGIRIWPEKVIGEGTQLSY
                     DLIWGKTEKKILFTNEGIYGSFNVKITPEFAAKLGSAIGAFLGKNKKVIVSRDTTRAS
                     RLIKRALTAGLLSMGVEVYDLEIASLPINKYSVVYENADLGIYVQISPHTDLQFNLIK
                     IFNRKGFELSNVERRKIENIFFRGDYPRQSAFEVGRLYYPTHNVESYIMFATQFVNKD
                     SIAQRQYNVIVDCFYGTTTHIFPSLLAAFSCKATVLRGQIREFNSSEDIRIESRDAIN
                     SIKNMAKLNSEIGVIVGPHGEQVTIIDEEGTILQWDDIYTLLGLLFIKHRHIQKLFMP
                     VISSSRLEHIAKDRGVQIERIAKTLSNDEFINIFSRDADTVYPYMIYEYDPMITFLLI
                     LELAALEGKKLSEIRKGLPCTNMYHTVLPCPVDAKAIIMREITATSNGHIELVDGIRI
                     VYDEGWILILPDPIQPAVHIYCEALTDEARDKIVEEYSVKIKKLTAKYT"
     gene            4311..5426
                     /locus_tag="PLJ75_08850"
     CDS             4311..5426
                     /locus_tag="PLJ75_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPD05360.1"
                     /translation="MKKTPHVNEERLIKTFIDLVKISSPSWEEKGVIDYIVNFSKSAG
                     VECITYPCNNSYNVWIKIPGDNSYVPILFSCHMDTVTPCENIQPLIKQNKITSDGNTI
                     LGADDKSAVAGFLEAILCIKENTLPHGNIEFLFSCAEETGLYGIKGFDLKQVQAKYAF
                     VFDSDGPVGGVVIAAPFHISMKIHVKGKAAHAGMEPEKGISAIRVISEIITNIPHGRI
                     DDETTVNVGTIKGGRATNIVAEEAECTLETRSRKKNKVLTIKKEITSIAKTIALRYKA
                     KITVTSNMDYEGYSINENDSIVSIAMKALQSIGKKPLLAVSGGGSDTNILNARGIKAV
                     NLSSGMRQVHTTKEYIAMQDLFDVPSLVLSIIQAAKK"
     gene            complement(5431..5874)
                     /locus_tag="PLJ75_08855"
     CDS             complement(5431..5874)
                     /locus_tag="PLJ75_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05361.1"
                     /translation="MIVLTFVIILQISFVIHVYYIISYITKKQDKDFKGFLFTSITNI
                     FLALFLSVFILIFPQELKEINLERILFIESGVVFLIMLSVKAKIASNIYKRAQDPQHF
                     HYSYFGKKVLHPSVVKMSELFTYFLTLPLTVICGAYFIVKLGCGK"
     gene            6026..7480
                     /locus_tag="PLJ75_08860"
     CDS             6026..7480
                     /locus_tag="PLJ75_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD05362.1"
                     /translation="MNQQHTYNLYEIFLNKSSQYPDDIFIVYNDEMIKYSDAFLNVSK
                     IAYALRKAGVANSSNVIMQIGNTPYFIYCFFAILQCNAMPVLVNPMARQYELKYYHDI
                     TTPACIITRSFIADRLTQQGLVSRGCEMIVIDDSSSFTSIKAILDADDYNMDYVPEEK
                     NGAAIIFTAAMDGKALGAVLTHTGIYHSAESVAWMLPHYASMMIAALPLFHAFGLTTS
                     LIIPLISKTEICLVDRFSPKKLFKLLLNRKVRYFVGVPAMYSIMKALFERDVDFSNIS
                     LWVSGGDYLSRELQEWYAEKGFVIRQGYGLTEASPIVSWNMPHIENKICSIGKPMPYN
                     EVRIVNEEGVMQPPRNNGEIIVKGINVINHYYNNNQMTQKYIKNGWLHTGDVGYYDND
                     GYLFITGRKKDMILKNGYNVYPKEVERILQYHPCIESVRVTGHVTFTGDSTIEMLVAE
                     IVCKGVKKLSVDEIKAWCIDNISLYKIPDTIIIR"
     gene            8201..9229
                     /locus_tag="PLJ75_08865"
     CDS             8201..9229
                     /locus_tag="PLJ75_08865"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPD05363.1"
                     /translation="MQHRVLQKGDLLDKEGKLIEKGYATSLIRRYNPEAISLYPLPFL
                     NKMRIKEWDYYGITTQDYFVSATISNIGYAGLLFIYYIDFKTKDYIEDTLITPFGKGF
                     TLGKTSDDDSMFKKGDIVFKCSKKNKEREIFINWPGFGKGKGFKAHLILQQPPGDSIV
                     MMTPIGNNRFYYNQKINCMPAEGAFILNGKEYGFTFNKALATLDWGRGVWEYKSYWNW
                     ASSSWFLPDGTRFGLNLGLGFGDLSYATENCFFIDGKIHKLDKVTFNYDNTNFMKPWY
                     FGSNDGRLGLTFTPFFERVARSNLLVITSEVHQLFGHYHGFVIGDNGKKINIAHCIGW
                     AEEHHARW"
     gene            9341..9670
                     /locus_tag="PLJ75_08870"
     CDS             9341..9670
                     /locus_tag="PLJ75_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05364.1"
                     /translation="MTDIYGDPVLFIKVMKLHGNEWIQDQQVDVFGKNNHWVISLGTH
                     LHGNTIKVLLATIIEEGQEMIIFESDPIALPASPEQLQETIPSYTKDIRDINNIHDLC
                     DDKRISS"
     gene            9667..11199
                     /locus_tag="PLJ75_08875"
     CDS             9667..11199
                     /locus_tag="PLJ75_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPD05365.1"
                     /translation="MSLEHPDKIKICIILHAHLPFVCDSSRDLPLEEIWLYQNIAECY
                     IPLVMMCNSLLKKNIQPSIALSLSPTLITMLQQDYYKYRFKQWCLQLCEFSDILKKHN
                     RKAVKALHFYDAFITDRLHYIENTDVLDDLHYLADTNAVELLTTAATHPFMPLVRHVP
                     YMMRLQIKLGIDTFYSRFGYKPDGFWLPELGYCYGMDSYLKDENIAYTIVNDKSILLS
                     NAVPACGNFSPSMTKSGLMVLPRDSVLSMKIWSGQGYPGNPYYREFHRDALYELQELS
                     PTPDNTLLGLKIYAVSGSDDKEYYNPDCIPEIVSKQVDDFIDAIYKRSEQVANITHKS
                     PVFILPFDAELFGHWWFEGPLFLELLIEKITQLHGVELIMPSDLLGSECATVDLVESS
                     WGKQADFSTWYNPLVRRNAAMLDEMIYEFLQYVNVKNEMLYQCAREILLAMSSDWLFM
                     ISTGSYTDYAGRRFSEHSDAARTLLTMLKNHESNETLIHERYKKYPIFPSIDRVLTRI
                     EL"
     gene            complement(11180..12451)
                     /locus_tag="PLJ75_08880"
     CDS             complement(11180..12451)
                     /locus_tag="PLJ75_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05366.1"
                     /translation="MATITMNNETDTIPFDQMQMQINKATEDVSVKCNTAIEPYIGFE
                     KGCTRISIPVPELSPALIIQSLGVIKDHIEHIRIHSFSEGYYAVQALNKNIFKTENIF
                     DNCKIEFSGLMTNFKIECTKKGNLSQEEINCIIALMQKAYSSMKENPVTRLIRRGASV
                     YTGTEGIDWNYIAGYEEVKRIIRESIILPLKNPSVYDNIARLTRKTFEYNKPKAILFE
                     GPPGVGKTTIARIIAREVQVPLIYVPVESIMSKWYGQSSQNLAEIFDASEDMGGSILF
                     IDEIDSLAGSRDQNMYEATRRILSVLLRRLDGIGAIANTMVIGATNRKQDLDHALINR
                     FDQSIHFPLPGENERASIFGNYAQHLNPEELMKLAHISNTLSGRTIKDLCEQTERRWA
                     RKLIIKNLPPSQPPFEYYYHTVKNFFTTQSG"
     gene            complement(12479..>13214)
                     /locus_tag="PLJ75_08885"
     CDS             complement(12479..>13214)
                     /locus_tag="PLJ75_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD05367.1"
                     /translation="KIAIVTGASRGIGQSIAKGLAAHGATVFCTSRKIEDLNKVKDEI
                     ELEGGKAHAIACHIGKLDDIQNLFKAVEERYGRLDILVNNAATNPYFGDVLHATADAW
                     DKTIDVNFKGYFFMSQYAANLMIKNKGGSIVNVASINGIRPAPFQGIYSMTKAGVIAM
                     TKSFAKELAPFNVRVNAILPGLTDTKFSSVMTQNEEILKLILPTIPMKRVANPDEMVG
                     AVVYLVSDAASYTTGATIVIDGGALA"
BASE COUNT         4190 a         2151 c         2675 g         4098 t
ORIGIN      
        1 cctctggatg caacaaataa ttttgctcat aaaatcccat ggttttgtgt taccattgca
       61 ctgtattcaa aagcgctgga agcggcggtt gccggcgttg tgtatgaacc gctcagggat
      121 gaatgctttt atgcgtggaa agacggcgga gcgtatgtaa atgaaaaacg tatctttgta
      181 tcgcagataa acgatatagg gatatcgatc attgctaccg gctttcccta taaaaaggac
      241 gatccttcat gcaataatgc aaagcaattt aataatgtat tgccgtatgt tcagggcatg
      301 cgtcgtatgg ggtctgctgc gattgatttg tcatatgttg catgtggaag atttgacggc
      361 tactgggaac cactgttata cccatgggat atgactgctg gcgctttact ggttgaggaa
      421 gctggcggca tggtgacaca atataacggc aacacatttg atccggaatt tcctgaaatc
      481 tgcgctacca atggactgat tcatcaacag ttgctggagc tcatcaataa atagggtacg
      541 gtattctgct caattttatt aaaaaaatgc ccgataaatg ggtaaggata attatagtaa
      601 aactgtctac ccactatggg ttgtgatggt taacaattat gattgcaggt ggcttatatg
      661 acaaaaaaaa ttatgtgtct atgctttgta ctggtggctt ccacattgct tgctcagaac
      721 acgcagagtg ttgatattcc agtacaatcc ttatatagca ataaggtgcg gataaaagat
      781 gtttatttta ataaaaaggt tgatctcacc ggtaaggggg aggtgctgga ggttgaagca
      841 atactggaaa atctcaccga tgatcctatt gatgtgtatg tcaatattat tgctacctat
      901 gaaaaagagg aaaaaacaaa atcaagtttt gagatgccaa tacctgaaaa agaaagagtt
      961 agaaactttg ttccttttcc agatgatatt gcaaattacc agtatcctga cgggaaagga
     1021 ggcaataagc ttaaagcata tccaaagaat acaaaatctg gcattgatcc attaacaggg
     1081 aaactatatc atctaccggt aaatgatata gtacttgtta gatctaccaa tctggcacca
     1141 tacaggaaag attttctatt ttttaattat gttactatcc ttgtttttga taacaatggt
     1201 actcccatct tacgtgaagt atttgaattg aaagggaaaa gaaagtagtt tgccaattca
     1261 gatacaataa cagttgttaa aaataccaat aaaaaggagt tgcccacagg gcaactcctt
     1321 ttgttatata agctttcgca cttttaatcc tgctatacca atctgggcta cctgctcttt
     1381 tgtaaactga gcaacattga tggtaatatc atacgatgcc gggtcatagg cattaaattt
     1441 tatgtattgt gtcataaact catgtcgctg tctatctttt tcttctatta gtttttcagc
     1501 agcaagccta tcttttattt tttggtattc cataaccttt tgtatgcggt attgtaaggg
     1561 agcaacaaag cgtagatgca agccatatcg tagacctttt gtaattatta cagaaccacg
     1621 ccctattaaa atagcatttc cgtgtattgc tatggtacgt attgtgttgg ctaatttctg
     1681 aaatactgct gcctgtggtg gcaagtctgc aagcatggta cgccataatt cggacatctc
     1741 ttcacgtctt cgggtatcaa tagtttcaac tattttttca gatatattat actgttgttc
     1801 aattttatta atgagatttt tatcatannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnntat atttattgca gcaacatggt atttcagata ttaccatgag tcttttttac
     1981 cttcctgaga atgttgttaa ctattttggt gatggcacct tctgggatgt ggcaatagat
     2041 tattctattg aagaaatacc attgggaaca gcaggagggg taaaattagc attgaaggat
     2101 tatgatgata catttattgt attgagtggt gatggacttt atgattttaa tgtacatgat
     2161 atcatacaat tccataagga aaaaagatca ttgtttacga tagctctggc acatgtgcct
     2221 gaccctgttg aatatggcat tgttgttacc catgaagatg gcactattga caggttttta
     2281 gaaaaaccat cgtggggcga ggtattcagc gacacagtga acacaggatt atatattatt
     2341 gaaccagaga ttattgataa atttgtacca gataatggta aatttgattt ttcgtatgat
     2401 ttatttcctc tactacaaaa gaagggaatt cctctttatg gctatgtaac tgatgggtat
     2461 tggtgtgata taggtaatct ggacgcgtat cgcaaggtac atcaggatat actcaatgga
     2521 attgtaaaga ttaattttcc aggtaaaaaa ataggtgaaa acatctgggc tggtgatgat
     2581 gttgaaattc atgcagatgc aatactaaag ggacctattg tgttaggaaa ttttgttaac
     2641 attaaaaaag gtgccgaggt atcagagtac agtgttattg gcgacaatac tgtcattcat
     2701 gaaaacgctt ccgtacgaag gagtgtgata ttacataata caatagtggg taccaaaact
     2761 gagatacggg gagctgtggt tggcaaacgc aatgttattg aagataatgt ctcaatttat
     2821 gaaggtgctg ttataagtga tgattgtcag ataggaaatg gtgctaaaat agccaccggt
     2881 atcagaatat ggcctgaaaa ggttattggt gaaggtaccc aattgagtta tgacctgatc
     2941 tggggaaaaa cggaaaaaaa gatattattc accaatgaag gaatatatgg ctcattcaat
     3001 gtaaaaatta cacctgaatt tgctgcaaaa ttagggtccg ctattggagc ttttttgggg
     3061 aaaaacaaga aggtcattgt cagcagggat acaacacggg catcacgatt aataaaaaga
     3121 gcgttgactg caggattact atccatgggg gtagaagtat atgaccttga gatagcaagt
     3181 ttaccaataa ataaatattc ggtggtttat gaaaatgctg atcttggtat atacgttcaa
     3241 atatcacccc ataccgattt gcaatttaac cttataaaaa tatttaatag aaaggggttt
     3301 gagttaagca atgttgaacg acgaaaaata gaaaatatat ttttccgtgg tgattatcca
     3361 cgccagagtg cttttgaagt aggcaggtta tattatccca ctcataatgt tgaatcatat
     3421 ataatgtttg ctacacaatt tgtaaacaaa gatagtatag cacaacgaca gtataatgta
     3481 atcgttgatt gtttttatgg aactacaaca catatatttc catctctttt agcagcattt
     3541 tcatgcaagg caacagtatt gcggggccag atacgagagt tcaatagttc cgaagacata
     3601 cgaattgaaa gtagagatgc aataaatagc ataaaaaata tggcaaaatt gaatagcgag
     3661 ataggagtta ttgtaggccc tcatggcgaa caggtaacaa ttattgatga agagggaaca
     3721 atcttgcagt gggatgatat ttatacatta ttgggactat tatttattaa acacagacac
     3781 attcaaaaac tattcatgcc ggttatatca tcgtcacgtc tggagcacat tgcaaaagat
     3841 agaggtgtac aaatagaacg aatagctaag acactatcca atgatgaatt tattaatatt
     3901 ttttcacgag atgcagatac ggtttacccg tacatgatat atgaatatga tcctatgata
     3961 acatttttat taattttaga actggcagca ttagaaggta aaaaattatc tgagataagg
     4021 aaaggattgc cctgtactaa tatgtaccat actgtgttac catgcccggt tgatgctaaa
     4081 gcaataatta tgcgtgagat tacagcaact tctaatggtc atattgaact ggtagacggt
     4141 ataaggatag tgtatgatga aggatggatt ttaatcttac ccgatccaat ccagcctgct
     4201 gtacatatat actgtgaggc gttgacagat gaggcgcggg ataaaatagt tgaagaatac
     4261 agtgttaaaa taaaaaagtt aaccgcaaaa tatacgtgag gcatatgaat atgaaaaaga
     4321 caccacatgt taatgaagaa agactgatta aaacttttat tgaccttgtc aaaatttctt
     4381 ctccgtcatg ggaggaaaaa ggtgttattg attatattgt taatttcagt aaatcagctg
     4441 gtgttgaatg cataacctat ccctgtaata attcgtataa tgtgtggata aagattccgg
     4501 gtgacaatag ttatgttccc atcctttttt catgccatat ggataccgtt accccctgtg
     4561 aaaatataca acctttaata aaacaaaata aaattacttc agatgggaat acaattttag
     4621 gtgccgatga taaatcggct gttgcaggat ttttggaagc aatactatgt attaaagaaa
     4681 atacactacc ccatgggaat attgaatttt tattctcatg tgctgaagaa accggattat
     4741 atggaataaa aggttttgat ctaaaacagg tgcaggcaaa atatgcattt gtttttgaca
     4801 gtgatggacc ggtagggggg gtggttattg cagcaccatt tcacatcagt atgaagatac
     4861 atgttaaagg gaaagctgct catgcaggga tggaaccgga aaagggtatc agcgcaatac
     4921 gggtaatatc agaaataatc actaacatcc cccatggaag aattgatgat gaaactaccg
     4981 tcaatgttgg aactataaaa ggtgggcgag caaccaacat tgttgcagaa gaagctgaat
     5041 gtactcttga gactcgttca cgtaaaaaaa ataaggtatt gaccataaaa aaagaaatta
     5101 cttctattgc taaaacaatt gcactacgat ataaagcaaa gattacggtc acaagtaata
     5161 tggattatga gggatatagt ataaacgaaa atgacagtat tgtttccata gctatgaagg
     5221 cattacaaag catcgggaaa aagccacttt tagctgtttc agggggaggt agtgatacca
     5281 atatattaaa tgcacgtggc ataaaagcag taaacctttc ttctggcatg cggcaggtgc
     5341 atactacaaa agaatatatc gccatgcagg atttgtttga tgttccttca ttggtactgt
     5401 ctataattca ggctgctaaa aaatgatgat ctatttgcca cagcctaatt ttacaataaa
     5461 ataagcacca catatcaccg ttagagggag tgttaaaaag tatgtaaaaa gttctgacat
     5521 cttaactacc gatggatgta aaactttttt tccaaaatat gaataatgga aatgctgggg
     5581 atcctgagca cgtttataaa tatttgaagc aatttttgct ttaacggaaa gcattattaa
     5641 aaaaacaaca cctgattcga taaaaagaat acgttccagg ttaatttctt taagttcctg
     5701 tggaaaaatt aagataaaaa cagacaggaa cagtgctaaa aatatatttg ttatggacgt
     5761 aaaaagaaag cccttgaaat ccttatcctg cttctttgta atataactta tgatataata
     5821 tacatgaatt acaaatgata tttgaaggat gatgacaaac gttaaaacaa tcataaatta
     5881 gcggctcttc tgtgatattt ttgtattata atatatttac tgtataagtc aagaaaatca
     5941 ttattataac tgatagattt acttgtattt tttcaaagaa tcatgtacaa taactatcgc
     6001 caaaaatata atggaaatta cttgtatgaa ccagcagcat acttataacc tttatgagat
     6061 ttttttaaac aaaagttcac aatatcctga tgacattttt attgtgtata atgatgaaat
     6121 gatcaaatac agtgatgctt ttttgaacgt tagcaagatt gcctatgccc tccgcaaggc
     6181 aggagttgcc aatagctcaa atgttatcat gcagatagga aatacaccat attttatcta
     6241 ttgttttttc gcaattttgc aatgtaatgc gatgcctgtt ttagtaaatc caatggcgcg
     6301 ccaatatgag cttaagtatt accatgacat cactacgcct gcatgtatca ttacccggag
     6361 tttcattgcg gataggttaa cacagcaagg tcttgtatcc aggggatgtg aaatgatcgt
     6421 cattgacgat agtagtagct ttacttcaat aaaagcaata cttgatgctg atgattataa
     6481 catggattat gttccagaag aaaaaaatgg tgctgcaata atttttactg ctgccatgga
     6541 tgggaaagca ttaggggctg tgttaactca taccgggata tatcacagtg cagaaagtgt
     6601 tgcgtggatg cttcctcact atgcctcaat gatgatagca gcgctaccat tgtttcatgc
     6661 ttttggactt actacctcac tcataatacc gctgatatca aagactgaaa tatgtcttgt
     6721 tgacaggttt tcacctaaaa aattatttaa attattgttg aacaggaaag tgcgttattt
     6781 tgtaggtgtg cctgctatgt atagtattat gaaagcgctt tttgaacgtg atgtagactt
     6841 ttcaaatatc agcttatggg taagtggtgg agattatctt tcacgagagc ttcaggagtg
     6901 gtatgctgaa aaaggctttg ttatacggca gggctatgga ttgactgaag cctcaccaat
     6961 tgttagctgg aatatgcctc atattgaaaa taaaatatgc tcaattggga aacccatgcc
     7021 ctataatgaa gtcaggatag tcaatgaaga aggagttatg caaccaccac gtaataatgg
     7081 cgaaatcatt gttaagggga ttaatgttat caaccattat tataacaaca accagatgac
     7141 acaaaaatat atcaaaaatg ggtggctcca taccggagat gtagggtatt atgataatga
     7201 tggttattta tttataacgg gcagaaaaaa ggatatgata ctgaaaaatg gttataatgt
     7261 atatccaaaa gaggttgaac gtatattgca gtatcatccg tgtatagaaa gtgtccgagt
     7321 gacaggacat gttacgttta ccggtgacag caccatcgaa atgcttgttg cagagatagt
     7381 gtgtaaaggt gtaaaaaaac tttcggttga tgagatcaaa gcatggtgta ttgataacat
     7441 ttcgttgtat aaaattcctg ataccataat tattagataa attatactat agatatgctt
     7501 aacaatgctt tgcagccttt tcataaacaa tagcatcatg atgctgtatt tttattccca
     7561 gctactgact gaaagcctgt cattggcatc tctattgaaa ctaaagacac tggaaaataa
     7621 aaacatttat atttatatag gaagttcaac gtaataaaca gtttatgcga gttggtttta
     7681 ttacgtgatt caaaaaataa aaaaaagagg taatcgggcg attacctcta gctctacaaa
     7741 agcatagcgg aatactttgg aaggaaattg ttgtgtacca ggattttccc agctcactct
     7801 tatatataag caaaccatat gccagcgtca agtaaaaaac ggattttttt ataaaaaaga
     7861 atgaaaaaaa ttaatttatc caaaattatt gtcattttca taaaaaatgt aagtataata
     7921 ggtacatttc tgaagagtgc ctatcgatat ttttggcaaa atcatcatcg atggagttta
     7981 ttcggtgaaa agagtggttc atcggtatag aaagtgtcct caaaatagga catatgttgc
     8041 ttaaaccgta caattggact aaaaatttca ctatagtgat attaattgca gaaagatgaa
     8101 aatgaaatag tgaatacata gtaaaaaaaa ttgcttgact ttatagtttt agtgctattt
     8161 cattctgtta tgaacaataa tgacaaacgg agtttattct atgcagcata gggtactaca
     8221 aaagggtgat ttgcttgata aagaaggaaa gctcattgaa aaaggatatg caacatcgct
     8281 gatacggcga tataatccgg aagctatttc gctatatcca ttaccttttc tcaacaaaat
     8341 gcgaattaaa gaatgggact attatggtat aaccacacaa gattattttg tatctgctac
     8401 aatttcaaat attggatatg caggattgct gtttatttat tatattgact ttaaaacaaa
     8461 ggattacata gaagatacgc ttatcacccc gtttggcaaa ggttttacat taggtaaaac
     8521 cagtgatgat gatagcatgt ttaaaaaggg ggatattgtt tttaaatgta gtaaaaaaaa
     8581 taaagaaaga gagattttta taaattggcc gggttttggg aaggggaagg gttttaaagc
     8641 gcacttgatt ttgcaacaac caccgggtga ttctattgtc atgatgacac caattggtaa
     8701 taaccgattc tattataatc aaaagataaa ctgcatgcct gcagagggag catttatcct
     8761 gaatggtaaa gaatatggtt ttacatttaa taaagcactg gcaacactgg attgggggag
     8821 aggagtatgg gaatataaaa gctactggaa ttgggcaagc agttcatggt tcttaccaga
     8881 tggaacacgg tttgggctta atttagggct tggttttggt gatctttcct atgctacaga
     8941 aaattgtttt tttattgatg ggaagataca taagcttgat aaggttacct tcaactacga
     9001 taataccaat tttatgaaac catggtattt tgggtctaat gatggaaggt tagggcttac
     9061 ttttacgccg ttttttgaaa gggtagcaag atcaaatctt ctggtaatta ccagtgaggt
     9121 gcaccagctc tttgggcatt atcatgggtt tgttatcggt gataatggaa aaaaaattaa
     9181 tattgcacat tgcattggct gggctgaaga gcatcatgca cgctggtaag ggagtatagc
     9241 catgtcagga tattttactt gtcatttacc ttcattttca ttaatgcatg ttcattggtc
     9301 aatagatagc cctaattggg agaaaattgt atcaggggca gtgacagata tttatggtga
     9361 cccggttcta tttatcaagg ttatgaaatt acatggaaat gagtggatac aggaccagca
     9421 ggttgatgtc tttgggaaga ataatcactg ggttatatcc ttaggtactc atttacatgg
     9481 caatacaata aaagtattgc ttgctacaat aattgaagaa ggccaggaaa tgattatttt
     9541 tgaatctgat cctatagcat taccagcttc acctgagcag ttacaggaaa ctattccgtc
     9601 ctatacaaaa gatatacggg atataaataa tattcatgat ttatgtgacg ataaacgcat
     9661 atcgtcatga gtttggaaca tcctgataaa ataaaaatct gtattatatt acatgcccac
     9721 cttccttttg tgtgcgatag ctctcgcgac cttccgcttg aagagatctg gttgtatcag
     9781 aatatagctg agtgttatat accacttgtt atgatgtgta attctcttct aaaaaaaaat
     9841 atacaaccat cgattgcact gtcgctcagt cccacactga taacgatgtt gcagcaggac
     9901 tattataaat accggtttaa acaatggtgt ttacagcttt gtgagttttc ggatatatta
     9961 aaaaagcaca atcgtaaagc agtaaaggca ttacattttt atgatgcctt tataactgat
    10021 agattgcatt atattgaaaa cactgatgtg ctggatgatt tacactatct tgccgatacc
    10081 aatgctgttg aactattaac aactgcggct actcacccct ttatgccact ggtgcgacat
    10141 gttccgtata tgatgcgttt gcagataaaa ctgggaattg atacctttta tagcagattt
    10201 ggttataaac cagatggctt ctggttgccc gagctgggct attgttacgg catggatagc
    10261 tatttgaagg atgaaaacat tgcttatacc attgttaatg ataaaagcat tttgctgagc
    10321 aatgctgttc cagcgtgtgg gaatttttcc ccatcaatga caaaaagcgg attgatggta
    10381 ctgccacgtg attctgttct ttcaatgaag atatggtcag ggcagggata ccctggcaat
    10441 ccatactacc gggaatttca tagggatgca ctctatgaat tacaagagct atcgcccaca
    10501 cctgataata cccttttagg gcttaagatt tatgcagtta gcggaagtga tgataaggaa
    10561 tattataatc cagactgtat tcctgagata gtaagtaaac aggtagatga ttttattgat
    10621 gcaatctata agcggtcaga gcaggttgca aacattaccc acaaatcccc tgtttttatt
    10681 cttccatttg atgccgagct ttttggacac tggtggtttg aagggccact ttttctggaa
    10741 ttgctcatag agaagattac acaattacat ggtgtagagc tgattatgcc ttctgatttg
    10801 ttgggcagtg aatgtgcgac agttgattta gttgagtcat cgtgggggaa acaggcagac
    10861 ttttcaacat ggtacaatcc gctggtgcgc aggaatgctg ccatgcttga tgagatgatc
    10921 tatgaatttt tacaatatgt gaacgtgaaa aatgagatgc tgtatcaatg tgcccgtgag
    10981 attttactgg ctatgtcatc tgactggttg tttatgatat caacaggcag ctacactgat
    11041 tatgctggca ggcgattttc tgaacatagc gatgctgcgc gaacactgct tacgatgcta
    11101 aaaaatcatg aaagtaatga aacattgatt catgaacggt ataaaaagta tccaatattc
    11161 ccctctattg acagagtact tacccggatt gagttgtgaa aaaattttta acggtgtgat
    11221 agtaatattc aaatggcggc tgtgaggggg gtaaattttt tataatgagc tttcgtgccc
    11281 atctgcgctc ggtctgctcg catagatctt tgatggttct tccggaaagt gtattgctta
    11341 tatgcgctaa cttcataagc tcttcagggt taagatgctg tgcatagttt ccaaaaatgc
    11401 ttgcgcgctc attttcgccg ggaaggggaa agtgaatgct ctgatcaaat cggtttataa
    11461 gcgcatgatc taaatcctgt tttcgatttg ttgcaccaat aaccatagta ttggcgatag
    11521 ctcctatacc atcaagacgc cgtaaaagca ccgataaaat gcgacgggtt gcttcataca
    11581 tgttctggtc acgagagcct gccagtgaat caatctcgtc aatgaaaagg attgatcctc
    11641 ccatatcttc gcttgcatca aatatctcgg ctaaattttg cgatgactgt ccgtaccatt
    11701 ttgacattat tgattcaact ggaacataga tgagaggcac ctgtacttct ctggcaatga
    11761 tacgtgctat ggtggttttg ccaacaccgg gaggcccttc aaaaagtatt gcttttggtt
    11821 tattgtattc aaatgttttg cgtgtcaacc gggcaatgtt gtcgtatact gaaggatttt
    11881 ttaaagggag tattattgat tcacgtatga tacgttttac ctcttcatat cctgcaatgt
    11941 aattccagtc aataccttct gtaccggtat atacggatgc accccgtcgt atgagccgtg
    12001 ttaccggatt ttctttcatt gacgaatatg ctttttgcat aagtgcaatg atgcagttaa
    12061 tctcttcctg cgatagattg cctttttttg tacattctat cttaaaattt gtcataagcc
    12121 ctgagaattc aatcttacag ttgtcaaaga tgttttcggt tttaaatata ttcttattta
    12181 atgcctgcac agcatagtac ccttcagaaa aagagtggat gcgtatatgt tcaatatgat
    12241 ctttgataac acccaggctt tgtattatga gtgcaggcga tagctccgga acaggtatac
    12301 tgatgcgcgt acatcctttt tcaaaaccaa tatatggctc aattgcagtg ttgcatttaa
    12361 ccgacacatc ctcagtggct ttgtttatct gcatctgcat ctggtcaaat gggatggtat
    12421 ctgtttcatt gttcatggtt atggttgcca tatataccag taaataaaac ttcaaaaatt
    12481 atgcaagcgc tcccccatct ataacaatag tagcccctgt tgtataactt gctgcgtcgg
    12541 atacaagata tactaccgct cccaccatct catcagggtt agctactcgc ttcattggta
    12601 ttgtcggcag aatgagtttt aaaatttctt cattttgtgt cataactgaa gaaaattttg
    12661 tatcggttaa acccggtaag attgcattga cgcgcacatt aaaaggtgcc agttctttag
    12721 caaacgattt tgtcatggca ataacacctg cctttgtcat agaataaatc ccctgaaatg
    12781 gtgcaggacg aataccattg attgaagcaa cattgacaat ggagcccccc ttatttttta
    12841 tcatcagatt tgcagcatac tggctcatga aaaagtaccc tttaaagtta acatcaatgg
    12901 tcttatccca tgcatcagcc gttgcatgta atacatcgcc aaaatatgga ttggttgctg
    12961 cattgttaac cagtatatcc aatctaccat agcgttcttc aacagcttta aaaagattct
    13021 gtatatcatc aagcttcccg atgtgacagg ctatggcatg tgccttaccg ccttctaatt
    13081 caatttcatc ttttacctta tttaaatctt ctatttttct gctggtacaa aaaactgttg
    13141 caccatgcgc tgctaaacct tttgctattg actgtccaat accacggctt gctccggtaa
    13201 caatagctat tttg
//
LOCUS       DAOOJH010000066        12947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4500_length_12947_cov_9.258843, whole genome shotgun sequence.
ACCESSION   DAOOJH010000066 DAOOJH010000000
VERSION     DAOOJH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4500_length_12947_cov_9.258843"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..1124
                     /locus_tag="PLJ75_08890"
     CDS             84..1124
                     /locus_tag="PLJ75_08890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPD05368.1"
                     /translation="MNGTTALIGCGRIGFLLEDDPLRTKPCTHYGGATKAKLTITYAC
                     DINKKRLSQFAQKAKLPIEHCYTDFQKLLEKENPECVIIASWTQTHATIGIEAAQHGA
                     KVIVCEKPIAQDIPSAKLLLEACSAHNTTLIINHERRYDYRYNFVKKLVENGSIGAIT
                     SVHGFVFTPSQAHIPQSGGGTLLHDGTHLIDTVHYLFGTIAQVQGHTKQYSSQSLYED
                     YALAHCLTHSGIHVILEAGGYRDYFLFELQIYGTGGAIVIGNGYLHVYTPEKSQFYTG
                     FNDLVEQPVHPKGKPDYFVQLYKEAKQCLKHPEQVTSSGIDGYRALEVIHAIYHSASQ
                     DGKTITLPLDCT"
     gene            complement(1187..1828)
                     /locus_tag="PLJ75_08895"
     CDS             complement(1187..1828)
                     /locus_tag="PLJ75_08895"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPD05369.1"
                     /translation="MFTGIIEEVGTVTSMVQSANGKLISISAHTVLEGTKAGDSLCID
                     GACQTVTQVGVDNFTVFASDVTLSITTLGMLKQGATVNLERAMQPQGRFGGHFVQGHV
                     DGTGTIVAVKKLPDGLSLMVDVPQSVSMFIVPKGSIAVDGISLTVVHKGNTIELYCIP
                     ETMANTTLSTKKRGDRVNLEVDIIAKYVYSMVQSIKGGDSSLETKLKDEGFWM"
     gene            complement(1831..3057)
                     /gene="ribD"
                     /locus_tag="PLJ75_08900"
     CDS             complement(1831..3057)
                     /gene="ribD"
                     /locus_tag="PLJ75_08900"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPD05370.1"
                     /translation="MENQKHNDYMRMALRLAFGRLGATSPNPAVGAVIVKNGQVVSTG
                     CTMEYGGDHAEVVAIKNASCNLQGAEMYVTLEPCCHQGKTPPCTNAIIASGIAVVYIP
                     MLDPNPVVAGRGMQTLQQAGVEVVVLHQCKQAAFDIVRPFIKSIARHKPFVIHKIAST
                     LDGRTATNTGDSRWISSEVSRLVVHRLRSCVDTIVVGSSTVEADNPRLTVRYDDFSDT
                     AQNFFSTRQYTFSGYPGSFLQGLLESWKTEVTHTARKVVIGLPGIFHDTYLAKEKDAC
                     FFIAQHQYNTLSHRDDYSIIRDLMDSGRIQCTHACHAVEQIHEVCDMLYNQGSMIVML
                     EGGAKLAGSFFNADAIDQCMYFYCPLIMGNGKGIIEGKGRPYINESIHLYDVSTVYLG
                     GDVLVSGYKEPYTCEA"
     gene            complement(3035..3913)
                     /locus_tag="PLJ75_08905"
     CDS             complement(3035..3913)
                     /locus_tag="PLJ75_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05371.1"
                     /translation="MPLWHAFIFFCSILVASCAIYAQVPDAYSYNSIAAFANWLVEQK
                     EYYRAYQEYRRLHAYYPDRYAYDSFVVASLYCQYEGKQYAEILSSANFTTTAPPWMWI
                     YIFDSAFKCNDSSVNHGIATIPSYEYSSDINEIIFKRKLAYCLYAGHDYQELSPTQSF
                     VDSYDVESLYAYSRFKRSQLKNPYAALAWGMVPGGGYVYSGNTEDGIVAAIVIGICSV
                     ITYFAIDTNNTGIAVFTGSIGVFFYGGSIAGGYLASIKANKAIMSELQAYLEEGLKFH
                     DDRAMMFERYGKPKTQ"
     gene            complement(3916..4806)
                     /gene="secF"
                     /locus_tag="PLJ75_08910"
     CDS             complement(3916..4806)
                     /gene="secF"
                     /locus_tag="PLJ75_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPD05372.1"
                     /translation="MEKIIPFFKYRFVAYSLTVVLIIIFSLQTYRNGGFNWGIDFVGG
                     AKITVKFQQGVDTAQIRKVLAHNAINATIQQVGKDEDNEYIIATKLTSRTENVEESTK
                     ALRTILQTNFNNAEVLSIETVGPAIGDFLKRSAGKLIALSIIIMTVYLAFRFELKYSV
                     GAMVALIHDIILTVLFCGFANIEINIPILAAILTIYGYSVNDTIIIFDRVRENLQITT
                     KQAFIDVIDKSITQTISRTLLTLLTTLFAVFALYMMGGEGLNEFATILLFGLSIGTFS
                     SLYIASPFVMYWEKLTSKQS"
     gene            complement(4819..6474)
                     /gene="secD"
                     /locus_tag="PLJ75_08915"
     CDS             complement(4819..6474)
                     /gene="secD"
                     /locus_tag="PLJ75_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPD05373.1"
                     /translation="MSRGLIYKGIFIVCIILFAIILILPTVGKNTLRVVMSTTATQED
                     IQAVKLRFPESNYTITARENEFFIEGYNLNVAVMNEVKQYHGVVDAQLLPHWAEKALL
                     AKKINLGLDLQGGSYLVLQANYHKIEEKTGKPLTNTEKNEITEQALELLRNRVDKFGV
                     AEPSIRPKGNDAIEIQLPGVKDPEAVKKAIGTTGQVEYRLVDDVYTQKANQWLKQYYA
                     GKALPEEQLELNKLADTLSNAIALPDTLELMFYYERHPQTGKLFAAYPMVLEKKVALA
                     GSDISKAWVATDEYQQLAVHFNTTPEGAAKFAEVTSEKNQGRKLAIVIDDKVRSAPSI
                     RVQINTGQAIIQGDFTHEEVTALARIIKEGALPVDLSIVEERTVGPTLGLDSIQSGVK
                     AFVVGVTGIMLFMFVYYKFSGLIADFGLLLNGIFMLALLSWLGFTLTLPGIAGFILTM
                     GMAVDANVLIYERIKEELRAGKTPRMAVTQGFSRAFWTIFDSNITTLLSAFVLSQFGT
                     GPIKGFAVTLSIGIICSMFVALYITRFIFEMITSRGTLQKLSI"
     gene            complement(6497..7132)
                     /locus_tag="PLJ75_08920"
     CDS             complement(6497..7132)
                     /locus_tag="PLJ75_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05374.1"
                     /translation="MKTYTNKLPLKLMCGVCLLFSTLMLYGPAIAQDQNPQTVKQPVA
                     EDTVVQNKTNQQELSPQQNVVEDTATTAEQAPAHEVDTTEKKVVIKEEPKKEAAKPQT
                     KAREVIDQKIQGNDTVTVGTNSLLSITDGTFRYSRIPGITIKENNEPILTVVKEEVPE
                     TVEEKGIFGLSKKASDRIAKIILVVIVIFVFILYRMRTKNTRGSVLRRFPK"
     gene            complement(7519..7845)
                     /gene="yajC"
                     /locus_tag="PLJ75_08925"
     CDS             complement(7519..7845)
                     /gene="yajC"
                     /locus_tag="PLJ75_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPD05375.1"
                     /translation="MRGIKVFPFEEIAYASNGQQSSSPWVSIIPLVLMIVIFYLLLIR
                     PQQKKEKDRMKMINQLKKGDKVLTSSGMIGVIVNIKPEENIITLKLAEGVKVDFVKSA
                     IQQKLS"
     gene            complement(8049..8720)
                     /locus_tag="PLJ75_08930"
     CDS             complement(8049..8720)
                     /locus_tag="PLJ75_08930"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPD05376.1"
                     /translation="MPLIKLVVGTTMVVMLACSGDVYYKNSGQKLLKTYKGNYAIVVS
                     KKNFALFVVSREKIIKKYTIAYGLNPDKKPKLYENDNRTPEGLYYVTEILSMDAPKHS
                     SSYKKLQGMNRVYFKSSKGHYKFGYPDVDLGDNAYGPRFYRLSYPNDNDIKRYEQAKI
                     HGALPLYNGKMPSIGGGIAIHGNNDPDSIGHLATSGCIRMLNDDVIELEQFIQIGTPV
                     YIVPD"
     gene            complement(8729..9190)
                     /locus_tag="PLJ75_08935"
     CDS             complement(8729..9190)
                     /locus_tag="PLJ75_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPD05377.1"
                     /translation="MRFNWIYTLLIFMVLLLCCSSLEKEQDTIAIRYVQLSLLYNYMI
                     QTSPDASQLQQQYKEIQSKLGTINEVVDGDERKNCIEKLNSINKEMEKQKKIFLTDIQ
                     QAVATVAKRKGYTIILGGGDTVVYAKGGYDITNEVLKELVATRLQKSPVTR"
     gene            complement(9193..11721)
                     /gene="bamA"
                     /locus_tag="PLJ75_08940"
     CDS             complement(9193..11721)
                     /gene="bamA"
                     /locus_tag="PLJ75_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPD05378.1"
                     /translation="MKKIGILVLSITFIAIFDGGFGQPSKYEGKIVKKIEFTGLFDVD
                     EDDLLDVMTTEVGFPLRSIEVREDIKKIFKTGKFENIIVEVQEHQDGVAVRFVCTERP
                     SINKIIFKGVEEINESTLKEELPIKEGEVYRKDKVEVSLIKIRNKYDIEGFFNAVVRY
                     KIEKVDEKDGKNAVNVIFIVDEGEDIKVQKISILGNTIFSLRTLKGLLVTEEEGLFTD
                     AKFTKVTYDEDKGKILGYYRESGYLDAEIVEDTVDYKWLDPETQEKRGIYITIKLREG
                     ERYYFDGYSVDGNEVFTKEDLMSQFEQIKIGQPFNDTLFQKDRQMISFIYATKGYIFA
                     RIIPERKITEKVVEVDGKKEKRKYIFTHFQIKEGPQAYIENIIIKGNIKTKDKVIRRE
                     LIVYEGELFNSYKMQLSRERVYNLGFFKEVNFDVRPGSKEGYMNLIVDVVEQPSGTIS
                     LGGGYGSTTGFSIFADIAENNLLGNGQRISLRFEYGPLRNSITLGFNEPWLFNKPIGF
                     NVSIFYILSTIEQSSIFTNSNQTAKHQTQQYGYSLGLSYRFWYYYGIGSVWHHAFKKV
                     VNATGNSTDSVFLEQAQGIQEKRKLTLYAYRDSKDNYLNPTKGWRAEFSASFTGGYLL
                     RGDDHWVMYSPDLYWYFSPFHLPFLKTHPCVVELRFNGTFITPPWYKSKMYEVQDPDK
                     NPWLESEDRLYIGGPETLRGWEVLDLDFPDSWRYGLFHRILYGAEFRVPIHPTFLWLV
                     FFFDAGSVWTDSFWEKSLEESTREIINEDKVNKLVYDIRDITDVDVMSYFKYSYGFGF
                     KIQLPMMPLRFWFGRKLAWVGKDEGYFKPLSGFQFQFGIGDMRF"
     gene            complement(11746..12174)
                     /locus_tag="PLJ75_08945"
     CDS             complement(11746..12174)
                     /locus_tag="PLJ75_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPD05379.1"
                     /translation="MDAKGIKRSIKVKSKFDYRSIIDITSLVDMTFLLLCFFMVTSSL
                     GSLSSITVHLPKAIQSGQMQQGNMVVSIDEKNEIFINDVKYQLADLLNEFKSRKASIK
                     NNVVIIRGDRNANYETIIAVMDILNQAGIARFTLATVKPK"
     gene            complement(12203..12844)
                     /locus_tag="PLJ75_08950"
     CDS             complement(12203..12844)
                     /locus_tag="PLJ75_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPD05380.1"
                     /translation="MYSDNYWISIFEAIPTWVMLLPIFICSIIGTAAIIERILFYNRI
                     NTDYAMHINNVAKEYSNNIQQALLQCKKVPGPISELIYNTLAFPASMKERERVITENV
                     YLTQKKIEKHVGIIATIATISPMFGLLGTVTGMMKSFSALSRVGNVAQDLLAYGVAEA
                     LITTALGLLVAIPAWIFYNYLVSRVERYAKDLEYVANALLDLPVYTLEPQIEK"
BASE COUNT         3889 a         2894 c         2163 g         4001 t
ORIGIN      
        1 aaaataaata ttatgagctt gatcagtaca accgccatgt taccgactat gagctaaata
       61 cctatctgcc cattttataa aacatgaatg gtacaactgc acttataggc tgtggcagga
      121 ttggttttct tctggaagat gatccactcc gcacaaaacc atgcacacac tatggcggag
      181 caacaaaagc taaacttacc ataacgtatg cctgtgatat caataaaaaa cggctatccc
      241 agtttgctca aaaagcaaag ctaccaatag agcattgcta cactgatttt caaaagcttc
      301 ttgaaaaaga aaatcctgaa tgtgtgatta ttgcttcatg gactcaaacg cacgccacca
      361 ttggcataga ggcagcacag catggtgcaa aggtcattgt ttgcgaaaaa ccaattgcac
      421 aggatattcc atcagctaaa ttattgcttg aagcatgcag tgcccataac acaacgctta
      481 ttatcaatca tgagcggcgc tacgactatc gatataattt tgtaaaaaag cttgttgaaa
      541 acggcagcat tggtgctatc acctcggtac atggatttgt gtttacacca tcgcaggctc
      601 atataccaca gtcaggaggg gggacactgc tgcatgatgg aacccacctt attgatacgg
      661 tgcattattt gtttggaact atcgcacaag tacaggggca taccaaacag tattcgtcgc
      721 agtcactata tgaagattat gcactggcgc attgtttaac gcattcgggt atacatgtca
      781 tccttgaggc agggggatac cgcgattact ttttgtttga gttacagata tacggaacag
      841 gcggtgcgat agttattggg aatggatatt tgcatgttta cacaccagag aaatcacagt
      901 tttatacagg cttcaatgac cttgttgaac aacccgtgca tcccaaaggg aaacccgatt
      961 attttgtaca gctttataaa gaagcaaagc aatgccttaa acatcctgaa caggtaacat
     1021 caagcggcat agatgggtat agagcattag aggttatcca tgcaatctac cattcagcct
     1081 cacaggatgg taaaaccatc acgcttcccc ttgattgtac ttgaagcagt aatgtatggt
     1141 tttgatttta atccggggta tatcacgggt tttattttca taaactttac atccagaatc
     1201 cctcatcttt tagctttgtt tcaagggaac tatcaccgcc ttttattgac tgcaccatgg
     1261 aataaacata tttagcaatg atatcaacct caaggttaac cctatcgccg cgtttctttg
     1321 tggaaagcgt agtattggcc atagtttccg ggatgcaata caattcaata gtgttaccct
     1381 tgtgcaccac cgttaatgat atgccatcaa cagcaattga acctttaggc acaataaaca
     1441 tgctaacact ttgcggaaca tcaaccatga gcgaaagccc atcaggcaat ttttttactg
     1501 caacaatcgt gcctgtgcca tccacatgcc cctgcacaaa atgtccgcca aacctgccct
     1561 gcggctgcat tgcacgttcc agattaaccg ttgccccctg cttcagcata ccaagcgttg
     1621 tgatggagag cgtaacatcg gatgcaaaca ccgtaaaatt atcaacaccc acctgcgtaa
     1681 cagtttgaca ggcaccatca atgcatagag aatcaccagc ttttgttccc tccagcacgg
     1741 tatgagcact gatggaaatt aatttcccat ttgctgattg caccattgat gtaacagtac
     1801 caacttcttc aataataccg gtaaacatta ttatgcctca catgtataag gttctttgta
     1861 gcctgaaaca agcacatcac ctcctaaata caccgtgctg acatcataca ggtgtataga
     1921 ctcattaata tagggacgcc ctttgccttc aataatgccc ttaccgttgc ccatgatcaa
     1981 tgggcagtaa aaatacatac actggtcaat agcatctgca ttaaaaaaag agcccgcaag
     2041 ctttgcacca ccttcaagca ttactatcat tgagccctga ttatatagca tgtcacaaac
     2101 ctcatggatc tgctcaacag catggcatgc atgggtacac tgtatcctgc cactatccat
     2161 taaatccctg ataatggagt aatcgtccct gtgcgatagt gtattatact gatgctgtgc
     2221 aataaaaaaa caggcatcct tttctttagc aagataggta tcatggaaaa taccaggcag
     2281 tccaatgaca acctttctgg cagtatgggt tacttcagtt ttccatgatt ccagcaatcc
     2341 ctgtagaaag ctaccgggat atcctgagaa tgtgtattgc ctggtgctaa aaaagttttg
     2401 agcagtatca gaaaagtcat catagcgcac ggttaggcgc ggattgtcag cctcaacggt
     2461 ggagctacca acaacaatgg tatcaacaca tgaccgcagc ctgtgtacaa caaggcgtga
     2521 aacctccgag cttatccatc gcgaatcacc tgtattggtt gcagttctgc catcaagcgt
     2581 tgatgcaatt ttatggataa caaatggctt gtgccgcgca atacttttga taaaaggacg
     2641 cactatatca aaggcagcct gcttgcattg atgtaaaaca acaacctcca cacctgcctg
     2701 ttgcaatgtt tgcattcccc gccccgcaac aactggattg ggatcgagca tgggaatata
     2761 tactacagca atacctgatg caataatggc attggtacag ggcggtgttt ttccctgatg
     2821 gcaacatggt tccagggtta catacatctc cgctccctgc aggttgcatg aagcattttt
     2881 tatagctaca acctctgcat ggtcaccgcc atattccatg gtacaaccag tcgacaccac
     2941 ctgaccattt tttacaatga ccgcaccaac agcaggattt ggcgaggttg ctcccaatct
     3001 gccaaatgca aggcgtaatg ccattctcat ataatcattg tgtttttggt tttccatacc
     3061 gttcaaacat cattgctcta tcgtcgtgaa atttcaagcc ttcttctaaa tatgcctgca
     3121 attcgctcat gatcgcttta tttgccttta tacttgcaag atacccgcct gcaatgctcc
     3181 ctccataaaa aaatacgcca atgctgccgg taaatacagc aatgccagta ttgttagtgt
     3241 caatggcaaa ataggtaata acactgcata taccgataac tatcgccgca actataccat
     3301 cctcggtatt gccgctgtac acatagccgc cgcccggcac catgccccac gcaagtgccg
     3361 catagggatt ttttagctgt gagcgtttaa aacggctata ggcatacagg ctttctacat
     3421 catagctgtc aacaaatgat tgtgtgggcg atagttcctg ataatcatgc cctgcataca
     3481 aacaataggc aagctttctt ttaaaaatta tttcattgat atcgctactg tattcatacg
     3541 atgggatagt tgctatacca tggttaaccg atgaatcatt gcatttgaat gcgctgtcaa
     3601 agatatagat ccacatccat ggaggtgctg tagtggtaaa gtttgcagag gatagtattt
     3661 cagcatactg ctttccttca tactggcagt acaacgaagc aacaacaaag ctatcatacg
     3721 catacctatc gggataatat gcatgtaaac gcctatactc ctgatacgca cggtagtact
     3781 ccttttgctc tacaagccag ttagcaaagg cagctatact gttatagcta taagcatccg
     3841 gtacctgtgc ataaatagca cacgaagcta ccagtatact gcaaaaaaag ataaaggcat
     3901 gccatagcgg cataattacg attgctttga tgttagtttt tcccagtaca taacaaatgg
     3961 tgatgcaatg tataatgatg aaaatgttcc aatagacaat ccaaagagca atatcgttgc
     4021 aaattcattt aagccttcac cacccatcat gtataaggca aacactgcaa aaagcgttgt
     4081 cagcagggta agcaatgttc tggatattgt ttgtgttatg gatttatcaa taacatcaat
     4141 aaaagcctgc tttgttgtaa tctgtaaatt ttcacgaaca cggtcaaaaa taatgatggt
     4201 gtcattaacc gaatagccat aaatagtaag gattgctgca aggataggga tgtttatttc
     4261 gatatttgca aagccacaaa aaagaactgt aaggataata tcatgaatca gcgccaccat
     4321 tgcaccaacg gaatatttaa gctcaaacct gaatgctaaa tacacggtca taataataat
     4381 tgaaagagca atgagcttcc ctgctgacct ttttaaaaaa tcacctatgg cgggacccac
     4441 tgtttcaatg ctcagcacct cagcattatt aaaattggtc tgtagtatcg tgcgtaaggc
     4501 tttggtgctc tcttccacat tttcggttct gcttgttagc tttgttgcaa taatatattc
     4561 gttatcttca tctttgccca cctgctgtat ggtagcgttt atcgcattgt gtgctagcac
     4621 ctttcgtatt tgcgcggtat ctacaccttg ttgaaatttt accgtaattt tagcaccacc
     4681 tacaaaatca ataccccagt taaaaccacc attgcgatat gtttgtaatg aaaaaataat
     4741 tatcaacact actgtaagtg aatacgcaac aaatctatac ttaaaaaatg gaatgatctt
     4801 ttccaatgtc agcacccctt agatacttag tttttgtaat gttccgcgtg aagttatcat
     4861 ttcaaagata aatctggtaa tatataatgc tacaaacatt gagcatataa tacctataga
     4921 aagcgtaaca gcaaacccct tgatggggcc cgtaccaaac tgtgaaagta caaatgctga
     4981 aagcagtgtg gtaatattag agtcaaaaat agtccagaat gctcgtgaaa agccctgtgt
     5041 taccgccatc cttggtgttt tcccggctct cagttcttct tttatacgct catagatgag
     5101 aacattggca tcaacagcca tacccattgt caatataaaa ccagcaatac ctggcagtgt
     5161 taatgtaaaa ccaagccatg acaaaagcgc cagcatgaaa ataccattga gcagcaaacc
     5221 aaaatcggct atgagccctg aaaatttata atacacaaac ataaaaagca taatgccggt
     5281 aacaccaaca acaaatgcct ttacaccgct ctggattgaa tcaagtccca gcgtaggacc
     5341 aaccgttcgc tcttctacta tggacaaatc taccggcaat gcgccttctt taattattcg
     5401 tgcaagcgca gttacctctt catgggtaaa gtcaccctgt ataattgcct gtcctgtatt
     5461 tatctgtaca cgtatagaag gcgcgctgcg gaccttatca tcaataacta tcgccagctt
     5521 acgcccctga ttcttttcgc tggtaacctc ggcaaacttt gctgcacctt ccggcgttgt
     5581 gttgaaatgt actgctaatt gttgatactc atcggtagcc acccacgctt tgcttatatc
     5641 actgccagcc agggcaactt ttttttccag aaccatagga tacgccgcaa agagcttgcc
     5701 agtttgggga tggcgttcat aataaaacat aagctccaga gtgtcaggaa gggctatggc
     5761 attcgaaagt gtatcagcca atttattcag ttcaagctgc tcctccggta aagccttacc
     5821 ggcataatat tgttttaacc actggttagc cttctgtgtg tagacatcat caaccaggcg
     5881 gtattcaacc tgccctgtgg ttccaatagc tttttttacc gcttcaggat ctttcacacc
     5941 cggaagctgt atttctatgg catcattccc tttgggacgt atggatggct cagccacacc
     6001 aaatttgtca acacggtttc taagcagctc cagcgcctgt tcagttatct catttttttc
     6061 agtattggtt aatggttttc ctgttttctc ttctatttta tggtaatttg cctgtaatac
     6121 caaatatgat ccgccctgca gatccaatcc taaatttatc ttctttgcaa gcaatgcttt
     6181 ctcagcccag tgaggcagca attgcgcatc aactacaccg tgatactgct tcacctcatt
     6241 cattactgca acgttgaggt tgtatccctc aatgaaaaat tcattttctc tggcagtaat
     6301 ggtatagttt gattcaggga agcgtaactt aaccgcctga atatcttcct gtgttgcggt
     6361 tgtactcata acaacccgta gtgtgttttt tccaactgtt ggtaaaataa ggattattgc
     6421 aaataatatt atacaaacta tgaaaatacc cttataaatt aatccccgtg acatgtatac
     6481 ctcatgtaat gtgatgttat ttcggaaatc tgcgcaatac acttccgcgt gtatttttgg
     6541 ttcgcatacg ataaagaata aatacaaata tgacaataac aacaagaata atcttagcaa
     6601 tcctgtcaga tgctttttta cttaatccaa atataccctt ttcttcaact gtttcgggca
     6661 cctcctcttt tacaacggtt aggatcggtt cattattttc ttttatagta atgcccggaa
     6721 tacgcgaata tctaaatgtt ccgtcggtga tactcaataa agaattggta cctacggtta
     6781 ccgtatcgtt accctgtatt ttctggtcaa tcacctctct ggcttttgtc tggggtttgg
     6841 cagcctcttt ttttggctcc tcttttataa caactttttt ttcagttgta tcaacttcgt
     6901 gtgctggtgc ttgttctgct gttgttgctg tatcttcaac tacattttgc tgtggcgata
     6961 gttcctgttg attggttttg ttttgcacta cagtgtcttc agctaccggt tgcttaacag
     7021 tttgaggatt ctgatcctga gcaattgccg gtccgtacag cataagagtt gaaaagagta
     7081 aacacacacc gcacatcaac tttaatggta gtttgttagt atatgttttc atatcttctc
     7141 caaaaaatac gattatcaga ctcaactatt gaccatcact tctatacaac tcagcaacag
     7201 catatgtgca aggatatcag gaatcgtttc tcgcctaaaa aaatgattat gcaccttacc
     7261 ccaactactc ttcttcacaa ggcgagggga gcattgtttc cctctctttg caacagcagg
     7321 attcttcact ccgttcagaa atgatatacc actatatcgt caaccccgtg cctggcccgg
     7381 aatatataac actatatcct gcatcatgat tcagtatgac tgtcgccaat tatcttcatc
     7441 atctacggtt ggccggcaac cactgtccat gtattaagat gacaaaataa tattaaaaaa
     7501 taatattaat gctatccttt aagaaagctt ttgttgtatg gcacttttta caaaatctac
     7561 ctttacccct tcagccaatt taagggttat aatattttct tcaggtttaa tatttacaat
     7621 aacaccaatc ataccgcttg aggtaaggac cttatcacct tttttaagct gatttatcat
     7681 cttcatacga tctttttcct tcttttgttg cggccgtatc aaaagaagat aaaatatcac
     7741 tatcatgagt actaaaggaa tgatacttac ccatgggctt gatgattgct gtccattgct
     7801 tgcatatgct atctcttcaa atgggaacac ttttatacct ctcattgtta atctttacta
     7861 taaaacattg caaccattat ggtataaaca gcaaatgtca atcagtttta ttgtttgaaa
     7921 cattttacat acgctgttaa acaaccactg attgcttgat tgctatacat aataaaaatc
     7981 agtatttaac ctgtatatcc tttttaagaa ctatcaccaa atgtcaacac aatttaccca
     8041 aattaatatt aatcaggtac tatataaacg ggtgtaccaa tctggataaa ctgctcaagc
     8101 tctatgacat catcatttaa catccgaata catccacttg ttgctaaatg ccctattgaa
     8161 tcaggatcat tattgccatg aatagctatt cctcccccaa ttgaaggcat cttgccatta
     8221 tacagcggca atgcaccatg tatctttgcc tgttcatatc gtttgatatc attatcattg
     8281 ggatatgaaa gacgataaaa ccgtgggcca tacgcattat cacctaaatc aacatcagga
     8341 tacccaaact tataatgtcc tttgcttgat ttaaaataaa ccctgttcat cccctgtaat
     8401 tttttatacg acgaggaatg ttttggtgca tccatactca agatctcagt tacataatac
     8461 aacccttcag gagttctatt atcattttca taaagctttg gctttttatc aggattaagc
     8521 ccatacgcta tggtatattt ttttataatc ttttcacggc ttactacaaa taaagcaaaa
     8581 tttttcttac tgacaactat cgcatagttg cccttatacg ttttcaaaag cttctgccct
     8641 gaatttttat aatacacgtc tccgctgcac gcaagcatca ctaccattgt agtacctaca
     8701 actaacttta ttaatggcat aattacctct atcgtgtaac cggtgatttt tgcagtcgtg
     8761 ttgccacaag ctcttttaat acctcattgg ttatatcata cccaccttta gcgtaaacaa
     8821 cggtatctcc tccccctaat ataatggtat atcctttccg ctttgcaaca gtagctactg
     8881 cctgctgtat atcagtgagg aaaattttct tttgtttttc catttcctta tttatactat
     8941 ttaatttttc tatacaattt ttcctttcat caccatccac cacttcattt attgtaccaa
     9001 gttttgactg tatctcctta tattgctgct gcaattgaga agcatccgga gaggtctgta
     9061 tcatatagtt gtacagaaga gacaactgaa catagcgaat agcaattgta tcctgctctt
     9121 tttctaatga agaacaacat aataacaata ccatgaaaat taatagtgta taaatccaat
     9181 taaaccgcat actcaaaaac gcatatctcc tataccaaac tgaaattgga agccgcttaa
     9241 aggtttaaaa taaccttcat ccttcccaac ccacgcaagt tttcgtccaa accagaatcg
     9301 caacggcatc attggcaact gtattttaaa gccaaatcca tatgagtatt taaaatatga
     9361 catgacatct acatcagtaa tatcccgtat gtcatacacc agtttattta ctttgtcttc
     9421 attgataatt tccctggtag attcctccag gctcttttcc cagaaactgt ctgtccatac
     9481 cgaaccagca tcaaagaaaa ataccagcca caaaaatgtg ggatggatag gaactctaaa
     9541 ttcagctccg taaagtatcc tgtgaaacaa tccataccgc catgaatcag gaaaatcaag
     9601 atccagcacc tcccagcctc gcaatgtttc aggaccacca atataaagcc tgtcttctga
     9661 ttcaagccat ggatttttat caggatcctg tacctcatac attttacttt tataccatgg
     9721 cggagttata aaagttccat taaatcgcaa ttcaacaaca cagggatgtg ttttaaggaa
     9781 cggtaaatgg aaaggcgaaa agtaccagta caaatcggga gagtacatca cccagtggtc
     9841 atcgccccgt agcagatagc cgccggtaaa tgatgctgaa aattctgcac gccatccttt
     9901 tgtaggattt aaataattat cttttgagtc acgatacgca tagagtgtca gctttcgttt
     9961 ttcctgaatt ccctgcgcct gttccaaaaa tacactatcg gtactgttac ccgtagcatt
    10021 gacaaccttt ttaaaggcat ggtgccatac tgaaccaatc ccataatagt accagaaacg
    10081 atagcttaat cccagtgagt aaccatactg ctgcgtttga tgctttgcag tttgatttga
    10141 attggtaaaa attgagctct gctcgatagt tgataaaata taaaaaattg aaacattgaa
    10201 acctattggt ttattaaaaa gccatggctc attgaaacct aacgtaatag agttacgcaa
    10261 agggccatac tcaaaacgca aggatatacg ctgtccatta cccagcaagt tattttcagc
    10321 aatatctgca aagatggaaa aacctgttgt agaaccataa ccaccaccaa gtgaaatggt
    10381 accagaaggt tgctcaacaa cgtcaacgat aagattcata tatccttctt ttgaaccagg
    10441 ccttacatca aaattgacct ccttaaaaaa tcctaaatta taaacacgtt cacgggataa
    10501 ctgcatcttg tacgaattga aaagctcacc ttcatacact attaactcac gtcgtataac
    10561 cttatctttg gttttaatat tgcccttaat aatgatgttt tcaatatagg cttgcggtcc
    10621 ttccttaatc tgaaaatgcg taaagatgta ttttcttttt tcttttttac catcaacttc
    10681 cactaccttt tcagttattt ttcgttcagg gataatgcgg gcaaatatat atcctttagt
    10741 agcataaata aagcttatca tctgcctatc tttttgaaac aatgtatcat tgaaaggctg
    10801 tccaatcttg atttgctcaa attgtgacat taaatcttct ttagtaaaaa cttcattccc
    10861 atcaacagaa tatccgtcaa agtaatatcg ttccccctca cgtaacttga tggtgatata
    10921 gatacccctc ttttcttgcg tttcagggtc aagccacttg taatcaacgg tatcctctac
    10981 tatctcggca tccagatatc ctgattcacg atagtaccca agtattttac ctttatcctc
    11041 atcataggta accttggtaa attttgcatc tgtaaatagc ccttcttcct cggttactaa
    11101 aagtcctttt aatgtacgca acgaaaagat agtattccct aatattgaaa tcttctgaac
    11161 ctttatatcc tctccttcat caacaatgaa tataacattg acagcatttt tcccatcttt
    11221 ttcatctact ttttcaattt tataccttac tacggcatta aaaaacccct cgatatcata
    11281 tttattacga atttttataa gacttacttc aaccttatct tttctatata cttcaccttc
    11341 tttgatcgga agctcttctt ttaacgttga ttcattaatc tcttcaacac ctttaaatat
    11401 tattttattt atcgatggtc gctcggtgca aacaaatctg actgctacac catcctgatg
    11461 ctcctgtacc tcaacaataa tattttcaaa tttacctgtt ttaaaaattt ttttaatatc
    11521 ctcgcgtacc tcaatactcc tgaggggaaa cccaacttca gtagtcataa catctaaaag
    11581 atcatcctca tcaacatcaa aaagtccggt aaactctatc ttttttacta tcttaccctc
    11641 atactttgaa ggttggccaa atccgccatc aaatattgct ataaaggtta ttgataaaac
    11701 cagtatccct atctttttca ttgagtaaat ccctataagc ttattttact ttggttttac
    11761 cgtagcaaga gtaaagcgtg ctattccagc ctgattaaga atatccataa ctgcaattat
    11821 tgtttcataa tttgcattcc gatcaccacg gattatcacc acattgttct ttattgaagc
    11881 tttcctgctt ttaaactcat tcaacaaatc agccaattga tattttacat cattgataaa
    11941 tatttcattt ttttcatcta tgctgactac catattcccc tgctgcatct gccctgattg
    12001 tatagctttt ggcaaatgaa cagtaatgga tgataatgat cccagacttg aagtaaccat
    12061 aaaaaaacaa agcaaaagaa aagtcatatc aacaagtgat gttatatcaa ttattgaacg
    12121 ataatcaaat ttacttttta ctttaatact gcgttttatt ccctttgcat ccatatcagt
    12181 aattattata gtgtatcaat cgttacttct ctatttgagg ctcaagagtg tacaccggca
    12241 gatcaagaag agcattagct acatactcta aatccttagc atagcgttca acacggctta
    12301 ccaggtaatt ataaaaaatc catgctggta ttgcaaccag taaccccaga gcagtagtaa
    12361 taagagcttc agccacacca tatgctaaaa gatcctgtgc aacattccct actctggata
    12421 aagcgctaaa ggatttcatc atgccggtaa cagttccaag caatccaaac ataggtgaaa
    12481 tcgttgcgat agttgcaata atcccaacat gcttttcaat ttttttttgt gtcagataaa
    12541 cattttcagt tataaccctt tccctttcct tcatgctggc aggaaaagct aacgtattgt
    12601 aaataagctc acttattggc ccgggcacct ttttgcattg cagcaatgcc tgctgtatat
    12661 tgttgctgta ttctttagct acattattaa tatgcattgc atagtcagta tttattctat
    12721 tgtaaaacag tattcgttca attatggctg ctgtcccaat tattgaacaa ataaaaatgg
    12781 gtaaaagcat tacccatgta ggaattgcct caaaaatact aatccaataa ttatcactgt
    12841 acatatatcg tccttacaac atctgaaatt ataactgtat tcgaatactt tttttacaac
    12901 aatatgcgta taccaaaaag tccctttata cctgattcag gataatg
//
LOCUS       DAOOJH010000067        12347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4726_length_12347_cov_10.036202, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000067 DAOOJH010000000
VERSION     DAOOJH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4726_length_12347_cov_10.036202"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLJ75_08955"
     CDS             complement(<1..186)
                     /locus_tag="PLJ75_08955"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD05381.1"
                     /translation="MVEQFFNIHAKEYCVTLAFLYGSWAHGLQRNNWDIDIAIMIEET
                     DEDEIFQKIIAMTLALSE"
     gene            complement(223..1491)
                     /locus_tag="PLJ75_08960"
     CDS             complement(223..1491)
                     /locus_tag="PLJ75_08960"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPD05382.1"
                     /translation="MSCTVIIGTQWGDEGKAKMIDFYSQKADIIVRYQGGANAGHTVV
                     VNGKKHVFHLIPSGILHKDTVCVIGNGVVVDPVQLIEECQLLENEGIDVRKRLFISDA
                     AHLLLPFHKELDGAMEEARSNKIGTTKRGIGPCYADKCLRVGIRVGDIFDDDYCKERI
                     TTALAIKNSQFERCYGREGFTFAEIYDLLVAFKDYAGALAINTPYYLYNEAKKGKNIL
                     LEGAQGNALDIDHGTYPFVTSSNPSIGGAIVGSGLNPFMIQEVVGITKAYVTRVGEGP
                     FPTEDKGSDGELIRQRGGEFGATTGRPRRCGWFDIELLKHTIRINGITTIALTKLDVL
                     SGFKTIKVAVGYSINGKKIDYFPSAMQDKIVPIYEEHPGWDEDIAGCTSFKALPVNAQ
                     NYVHYIQDTLQVPISIISVGPDRVNTFEIK"
     gene            complement(1582..2184)
                     /locus_tag="PLJ75_08965"
     CDS             complement(1582..2184)
                     /locus_tag="PLJ75_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPD05383.1"
                     /translation="MEQLYKKALNLSLFTIAYNIIEGIVSVVAGALAGSVALVGFGLD
                     SFVESLSGFIMIWRFKKHGKVTEQEEEVIENKALRYVGYTFIILSFYIAYESISKLYY
                     KEIPSPSFIGIIIAVASIVVMPVLYYKKIKTAKAINSKSLSSDAKETLACTFLSLALL
                     IGLGLNYLKGWWQADPVVGLVVTIYLIKEGIEILSGEEED"
     gene            2688..3037
                     /gene="ssrA"
                     /locus_tag="PLJ75_08970"
     tmRNA           2688..3037
                     /gene="ssrA"
                     /locus_tag="PLJ75_08970"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3126..>3455)
                     /locus_tag="PLJ75_08975"
     CDS             complement(3126..>3455)
                     /locus_tag="PLJ75_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05384.1"
                     /translation="DGDFLYYKATGIVTSYCTKNKAELQLIFEKGIDLLAIYELYGVV
                     SYGMGFVDYDSVFVIIDKNLKIVYFDYNHDRKNTSEFSTQVLWDLLLNEMNTRCVRTL
                     MKIGFLD"
     assembly_gap    3456..3555
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3556..4031)
                     /locus_tag="PLJ75_08980"
     CDS             complement(<3556..4031)
                     /locus_tag="PLJ75_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPD05385.1"
                     /translation="MKSVIFAGVGGQGVILAGKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCHVCFGEKVYSPLIEKGTADFVIALELLEAMRKLEYLSTNGTLIVNKFKVNPAP
                     VEAGLAKYPDDIESWLCSNIGKCHVVDTAKVLKEAGSNKAVNIVMVGILSNFLEFD"
     gene            complement(4042..>4680)
                     /locus_tag="PLJ75_08985"
     CDS             complement(4042..>4680)
                     /locus_tag="PLJ75_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD05386.1"
                     /translation="GELTPEIIDMAINKKMPKNEIPLYSGNIPGRPPALCKGCPHGFV
                     FEVLKKLDVVVNGDIGCYTLAVLPPYSAMHSQGCMGASIGMHSGFEKARGDEMARNSV
                     AVIGDSTFVHSGITPLIDLVYNKGTGTVIILDNRVTAMTGHQDHPATGKTLTGEDTHE
                     LDLELLCKAVGVKRVRKLDPKNMDEFEKAVKEEIAAKEPSVIISLRKCILKK"
     assembly_gap    4681..4780
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4808..5239)
                     /locus_tag="PLJ75_08990"
     CDS             complement(4808..5239)
                     /locus_tag="PLJ75_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HPD05387.1"
                     /translation="MNVTQLSLFLENKPGHLLNALKVIADENINILTLTIAEVSDFGI
                     VRMIVTKPQKAIEALRSHKFVCSPTDVLAIELDDKPGSLLQALEIFAKRNLNIEYMYA
                     FTEKRGDKAVMIFKFSDIETAKKSLLEEGFNVIKKIDLIGE"
     gene            complement(5347..6648)
                     /locus_tag="PLJ75_08995"
     CDS             complement(5347..6648)
                     /locus_tag="PLJ75_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPD05388.1"
                     /translation="MFNKEYESMEVKKLKELQLERLRWSLKHAYNNVAHYKNAFDKAR
                     FNPDKFSSLDDLKHVPFLMKQTMRDSYPYGLFAVPLEKVIRIHSSSGTTGNATVAGYT
                     RNDIEVWAELIARSIVGAGGSEKDIVQDAYGYGLFTGGLGLHYGAEKLGATVIPVSAG
                     NTERQLMLIKDFGTTILVCTPSYAVNMADYAEKKMPDYDFKKTKLKAGIFGAEPWTQS
                     MRKEIENRMGIDAYDIYGLSEVMGPGVSCECSQKSGLHIFEDHFYVEVIDPETGEVLP
                     EGEQGEIVYTSLTKEACPVIRYRSRDITRIYRDSCKCGRTMIKMEKVTGRSDDMLIIR
                     GVNVFPMQIEAVLMEVEGTEPHYQIIVDRKGALDDVEIKVEVNEKIFTDQLGVLKGIT
                     ERIEQRIRSVLGISAKITLVEPSTLQRSEGKAKRVIDKRNL"
     gene            6841..7668
                     /locus_tag="PLJ75_09000"
     CDS             6841..7668
                     /locus_tag="PLJ75_09000"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD05389.1"
                     /translation="MQDYLKLFMITVTILLCITEPTFPSDYIQNLKNGTWEQRITAMN
                     TLGYSGNKKAFWYLVKYLDQQFQSTNQNLAIRVRQTAALSLGRLKDDRAIPYLVEQYK
                     KEQDPEVKRAILFALRFFKPNQDTIAIVKQGLSDSNKDIQWEALIVASIYCDSSLATD
                     VAQLGKDDPEINVISAYIQYMINQNKSENESIMRKSLQHKNPSVRYWASHFLTKSLGT
                     AALEDIMKAQEIESVWWVANEMEDNIYILARKKFNDEMVEQLGSFRFVIDKKDKQVQ"
     gene            complement(7675..8190)
                     /gene="lepB"
                     /locus_tag="PLJ75_09005"
     CDS             complement(7675..8190)
                     /gene="lepB"
                     /locus_tag="PLJ75_09005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD05390.1"
                     /translation="METKDSFNIFAKVLFIFAGLIFGFILIRLFFIPVKINNVIMEPA
                     LHNNDYAWFYKTRKVDSGSIVLYKSPLSGKQVGRVIAKSGDTIEIINKRIYVNGIPLH
                     IHWKIVQKDTRTFPPRFTARDNLAAITVPNGHIFVICDNWDIPTDSRLFGTIPLNAIV
                     GKYIWKLSPKL"
     gene            complement(8212..9393)
                     /locus_tag="PLJ75_09010"
     CDS             complement(8212..9393)
                     /locus_tag="PLJ75_09010"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPD05391.1"
                     /translation="MSVATHISEAMIHSSWIRKMFEEGAKLKAQFGEENVYDFSLGDP
                     DVPQPESFYTTLKALASKPDPKAHGYMPNAGFTTTRDAIAKKVAKDHHVDIQGKHVIM
                     TCGAAGGLNVALKTILNPGEEVIVPKPCFAEYQFYISNHNGVITLVSTNADFSLDVDA
                     LEKAINDKTRAVLINSPNNPTGVIYSHDDITRLSSLLERYSNKYGKPIYLLSDEPYRE
                     LIYDGSTVPSILSHYNYSMVVSSFSKTMSLPGERIGYIAINPSMPEADIIMAGLILCN
                     RILGFVNAPALMQRIVERIAFDRVDVNAYAVRRDMFCNGLKDAGYSFITPKGAFYVFC
                     KSPIEDDVIFVKHLLNFNILAVPGVGFGGPGYFRLAFCVPEKVIEKSIPQFKKALEML
                     K"
     assembly_gap    9574..9673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9766..11586)
                     /locus_tag="PLJ75_09015"
     CDS             complement(9766..11586)
                     /locus_tag="PLJ75_09015"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPD05392.1"
                     /translation="MKVHELKHQSLTKWIQDASNLMKPDDVYVCDGSKGEYDMLAQQM
                     VDEGLAVQLNQKKRANCLLFRSDPSDVARVENRTFIASKTQEAAGPTNNWIDPVELKQ
                     TMTDLYRGCMRGRIMYVIPFSMGPVGSSIAKIGVEITDSPYVVINMHIMTRVGSKVLD
                     VLGTEGEFVPCWHSVGKPLTKGESDNGKWPCAPLEKKYISHFPETREIWSYGSGYGGN
                     ALLGKKCLALRIASVMAHDEGWLAEHMLILKITNPEGKAKYIAGAFPSACGKTNLAML
                     IPTIPGWTVETVGDDIAWLKFGSDGKLYAINPEAGFFGVAPGTSEESNYNAMKTIEKN
                     TIFTNVALTEDLNVWWEGIGYEPGGKLIDWLGNEWIYDKNNKDQKPAAHPNSRFTTPA
                     NQCPSIAKEWEDPDGVPISAILFGGRRAQTIPLVVESKNWLHGVFLGSIVGSEVTAAA
                     LDLTIGQVRRDPFAMLPFCGYHMGDYFKHWINIEKKTTSEKLPKIFFVNWFRKTDDGK
                     WLWPGYGENSRVLKWIFERCDGSGKAVDTPVGYMPAPDAIDKPASVSNQDMEILLKVD
                     REGWLREVDDVRNNHYPKFGNHLPQELYELLDTMESLLKA"
     gene            complement(11738..12040)
                     /locus_tag="PLJ75_09020"
     CDS             complement(11738..12040)
                     /locus_tag="PLJ75_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05393.1"
                     /translation="MVYSFDKDKTGYFLKIEGDLDLYSSYNLKEDIKAIFLKEPENII
                     IDLSHLNYIDSAGIGFLIDIKKNATQNNKLCDFKNIPSDILKLFHSIRVDVILHIK"
BASE COUNT         3712 a         2601 c         1839 g         3895 t
ORIGIN      
        1 ttctgacaat gctaatgtca ttgcaataat tttttgaaat atttcatcct catctgtttc
       61 ttctatcata atggcaatat caatatccca attattccgt tgtaatccat gcgcccatga
      121 gccatagaga aaagccagcg ttacacaata ttcttttgca tgaatattaa aaaattgctc
      181 caccatgttg agtagttatt catttgtcat aagtactcag catcatttta tttcaaaagt
      241 attgacccta tccggaccta ccgagattat tgaaatgggc acctgcaatg tatcctgaat
      301 ataatgcaca tagttttgtg catttaccgg caatgcttta aacgatgtac acccggcaat
      361 atcctcatcc cacccgggat gctcttcgta tataggtact atcttatcct gcatcgctga
      421 agggaaatag tcaatttttt tgccatttat ggaatatcct actgcaacct ttattgtttt
      481 aaaaccagat aaaacatcca gtttagttaa agctatggtt gttatcccat ttatccgtat
      541 ggtatgcttt aatagctcaa tatcaaacca gccacaccgc cgtggacggc cggtagttgc
      601 gccaaactct ccaccacgtt gtcgtattaa ctcaccatcg cttcctttgt cctcggtagg
      661 gaatggcccc tccccaacac gggtcacata agccttggta attccaacaa cctcctgtat
      721 cataaaaggg tttaatccag aaccaactat cgccccgcca atgcttggat tggatgaagt
      781 aacaaatgga taggtgccat gatcaatatc cagggcattg ccctgagcac cttcaagcaa
      841 tatgttttta ccctttttag cttcattgta caggtaatag ggggtattga tggctaaagc
      901 ccctgcatag tctttaaatg ccacaagcag atcatatatt tcagcaaagg taaagccttc
      961 tctaccataa catcgctcaa attgagagtt ctttattgcc aatgcagtgg taatgcgttc
     1021 tttacaatag tcatcatcaa agatatctcc aacccttata cccacacgta aacacttgtc
     1081 agcataacag gggccaatgc cacgtttggt ggttccaatc ttattggaac gtgcctcttc
     1141 cattgctcca tccagctctt tatggaatgg caacaggaga tgcgccgcat cggatatgaa
     1201 aaggcgtttt cgcacatcaa tgccttcatt ttcaagcaac tgacattctt caataagctg
     1261 cacaggatca acaaccacac cattgccaat gacacaaacc gtgtccttgt gcaaaatacc
     1321 tgatggtata agatgaaaaa catgcttttt gccattgact accactgtat gtcctgcatt
     1381 agcaccaccc tgataacgaa cgataatatc ggctttctgc gaataaaaat caatcatctt
     1441 ggctttgcct tcatcacccc actgtgtgcc tataataact gtgcaactca tagttacctc
     1501 atatattata taaagttgtt tttaacgcca accttaccac catagaaaaa acaaaaaaaa
     1561 taacaagctt ttttctactt atcaatcttc ttcttctcca gacaaaattt ctatcccctc
     1621 ttttataaga tatattgtaa ctactaatcc gaccacagga tctgcctgcc accacccttt
     1681 tagataatta agtcctaatc caataagcag agccagcgat agaaaagtac aggctaaggt
     1741 ttcttttgca tctgatgata aacttttact atttattgct tttgccgtct ttattttttt
     1801 ataatacaat actggcataa caactattga ggcaactgca ataataatcc caataaaact
     1861 tgggctgggg atttctttat aatacaattt acttattgat tcatatgcta tgtaaaaact
     1921 taaaataata aaggtatagc caacataccg taaagcttta ttttcaatta cttcttcctc
     1981 ttgttctgtt actttaccat gttttttaaa tctccatatc ataataaaac cagataatga
     2041 ttcaacaaag ctatccagtc caaaaccaac cagcgcaaca ctgcctgcaa gtgcaccagc
     2101 aacaactgaa acaatccctt caataatatt ataggcaatt gtaaatagtg aaagattaag
     2161 tgctttttta tataattgtt ccatagttaa acctctcaat taatttttat gtgatgctgt
     2221 tataccttcc cacttcattt tcattcgttc acaataaatg cacccccaac agcatatcaa
     2281 caccaggcat tataccgtaa atcatttttt ttattatgtt cgatagttat tgttaatcat
     2341 atcacagtgt gaaatatatc acttcgtata attgccttcc attcatgatg agcaataatt
     2401 ctaatctttt tattctatat ttgaacctcc aatgaattat tttagattat tccctattaa
     2461 ttattgaagc gctcgctaca atacctcttt tgcccactat gatagcttcc aagtaatctc
     2521 gtgtaatcga ccattacctg taaaaggtat gacaatagct tcgatatagt gttccaatga
     2581 aaaacttcta cttacttact aaaaatcatt atagattgta acatcttcaa aaaattattc
     2641 ctataaatat tgacaattat gaatcttatt gtatattata tagtgcaggg gacgattttg
     2701 gtttcgactg caagtatgtg tctgtagctg catgtcgagt ttctgctggc tcgttaaaac
     2761 tggcaggcta aaataagtgc aaataacgat ttagctctcg cagcttaatc cctgcgggcg
     2821 tctttacctg ttctgctcct ctggggatgg taaaggcgta agatacagag gatagctatt
     2881 aaaaattgat cctgatttaa tagcaaattt aaaaggatct gctcattgag aagggttcct
     2941 gttccccaaa ccaatgaaaa gattaacgaa caggataaac atgtagaggc tgcaggtgtg
     3001 tattgtagga cgcgggttcg actcccgccg tctccaagtt tatttaattt ttattatacc
     3061 aattacaaca ctggaaaaaa ctaaactttt ttatgagcaa taagtttgtc aatatctcat
     3121 agcatttaat ctaaaaaacc tattttcatc aatgtacgca cacatcgtgt attcatctca
     3181 ttcagcaata aatcccataa cacctgcgta gaaaattcac tggtattttt tctatcatgg
     3241 ttataatcaa aatatacaat cttcaagttt ttatcaataa tgacaaaaac cgaatcataa
     3301 tctacaaatc ccataccata tgataccaca ccatacagtt catagatagc cagcaaatca
     3361 ataccctttt caaatattag ttgcagttct gctttattct tagtgcaata cgaagtaaca
     3421 atacctgttg ctttatagta taaaaaatca ccatcnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnntgatc aaactccaaa aaattagata aaatccctac catcactata
     3601 ttcaccgctt tgtttgatcc tgcttccttt aataccttag ctgtatccac aacatggcat
     3661 ttcccaatat tactacataa ccagctctca atatcatcag gatactttgc taaacctgcc
     3721 tcaactggcg caggattaac cttaaattta tttacaataa gcgtcccatt tgttgataaa
     3781 tattctaatt tcctcattgc ttccagtaat tccagcgcta taacaaaatc ggctgtacct
     3841 ttttcaatca atggtgaata caccttttcc ccaaaacata catgacaatc aacactaccg
     3901 cctcgctggg ccatgccatg tacttcagat tccttaacat catatcctgc attcatggca
     3961 acctgcatta atatcttacc cgcaaggata accccctgac caccaactcc cgcaaaaatg
     4021 acacttttca taaatattcc cctatttttt caaaatacat ttccgcaatg aaataataac
     4081 cgaaggttct ttagctgcaa tttcttcttt tacagctttt tcaaactcgt ccatattttt
     4141 tggatcaagt tttctaaccc gctttacccc aacagctttg cacaaaagtt caagatccag
     4201 ctcgtgtgta tcctctcccg taagtgtttt ccctgttgca ggatgatctt gatgcccggt
     4261 cattgcagtt accctattat ccagtataat aaccgtacca gttcctttat tatatactaa
     4321 atcaattaat ggtgtaatac ccgaatgcac aaaggttgaa tcaccaatga cagcaacact
     4381 atttcgtgcc atctcatcac cgcgggcttt ttcaaatcct gaatgcatac caatgctggc
     4441 acccatacat ccctgtgagt gcatcgcaga atatggcggc aatactgcca atgtataaca
     4501 accaatatcg ccattaacaa caacatctaa ttttttcagc acctcaaata caaaaccatg
     4561 aggacatcct ttacataatg ctggaggtct tcctggaata ttcccggaat atagtggtat
     4621 ttcattttta ggcatctttt tattgatagc catatcgatt atttcaggag ttaattctcc
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccagcataa cttttttttg
     4801 cattgcatta ctccccaatt aaatcaattt tttttatcac attaaatccc tcttcaagaa
     4861 gcgatttttt tgctgtttca atatctgaaa acttaaaaat cataacagcc ttatcaccac
     4921 gtttctcagt aaatgcatac atatattcaa tatttaaatt tcgtttagca aatatttcaa
     4981 gtgcttgtag caatgaacct ggtttatcat ctaattcaat agccagtaca tcggttgggg
     5041 aacaaacaaa tttatgagaa cgcaaagctt ctattgcttt ttgaggttta gtaactatca
     5101 tccgtacaat accaaaatct gatacttctg caatagttag cgtaaggata ttaatatttt
     5161 catctgctat tacttttaat gcattcagta ggtgtcctgg cttattctcc aaaaaaagtg
     5221 ataattgcgt tacattcatg gttattctcc taaaaataaa tttacgtggg cacaccacat
     5281 tgagttaatt ctacgaatat tttttgcttt atgaatgaga tcctgcatca tttacatctt
     5341 tattctctac aaattacgtt tgtcaataac gcgttttgct ttaccttcac tacgctgcaa
     5401 ggtacttggt tctaccaatg taatcttagc actgattccc aatacgcttc tgatacgctg
     5461 ttcaatccgc tcagttattc cctttaatac acccagttga tcggtgaata ttttttcatt
     5521 tacctccacc tttatttcaa catcatcaag agcacctttg cgatccacga taatctggta
     5581 atgaggttca gtaccttcaa cttccatgag gactgcttct atctgcatgg ggaatacatt
     5641 aacgcccctg atgataagca tatcatcact tcttcccgta accttttcca ttttaatcat
     5701 tgtcctacca cacttacagg agtcccgata tattctggta atgtctcgtg acctataacg
     5761 aataaccggg catgcctctt tcgtaagtga agtatatact atttcaccct gttcgccttc
     5821 tggcaatacc tctcctgttt cagggtctat tacctcaaca taaaaatggt cttcaaatat
     5881 atgcaatcct gacttctggc tgcattcgca tgatacgcca ggacccatga cctcacttaa
     5941 tccatatata tcatatgcat caattcccat acgattttct atctcttttc gcatactttg
     6001 agtccatggt tcagctccaa aaattcctgc tttaagcttt gtttttttaa aatcataatc
     6061 aggcattttc ttctcagcat aatctgccat attaactgca tacgaaggag tacaaacgag
     6121 gatagttgta ccaaaatcct ttatcaacat caactgtcgc tcggtattgc ctgctgatac
     6181 cggtataaca gttgcaccca gcttttcagc accataatgc aatcccaatc cgccagtaaa
     6241 caatccataa ccataagcat cctgaactat atccttttct gaacctcctg caccaactat
     6301 ggatcgagct atcagctcag cccacacctc aatatcattt cttgtatacc cggctacggt
     6361 tgcattcccc gtagtacctg atgaagaatg tatccgaata accttttcca atggcactgc
     6421 aaataaacca taaggatagc tatctcgcat cgtctgcttc atgagaaaag gaacatgctt
     6481 caaatcatca agagaggaaa atttatcggg attaaacctt gccttatcaa aagcattctt
     6541 ataatgggca acattattat aggcatgttt aagcgaccat cgtaacctct ccagttgaag
     6601 ttcttttaac ttttttactt ccattgattc atattcttta ttgaacattg ccagctcctt
     6661 ttatatggct atctctattt aaaattttta agatggaaga ttttattaat aattattaaa
     6721 attgtcaata aacttgcaaa tgtttaaaaa aaaatttaaa aaataattga gttttttatg
     6781 gtgatgttgt gaaatttttt atattagtaa atatctattc tattctggag cgtaccaatt
     6841 atgcaagact atttaaaact ttttatgatt actgtcacta ttctgttgtg tataacagaa
     6901 ccaacttttc ccagcgatta tatccagaat ctaaaaaacg gcacatggga gcaacgaatc
     6961 acagccatga acacactcgg atattcaggg aataaaaaag ccttctggta tctggtaaaa
     7021 tatctggatc aacagttcca atcaactaat caaaatttag ctatacgggt acgccagact
     7081 gcagcactgt ctttaggcag gcttaaagat gatcgcgcca ttccctatct tgttgaacaa
     7141 tacaaaaagg aacaggatcc cgaggttaaa agggctatcc tttttgctct ccgttttttt
     7201 aaaccaaatc aggatactat tgctattgtt aagcaggggc tgtccgacag caataaagat
     7261 attcagtggg aagctcttat agttgcaagt atctattgtg attcgtcgtt agcaacagat
     7321 gttgcccaat taggcaagga tgatcctgaa attaacgtaa tctctgcata cattcagtac
     7381 atgatcaatc aaaacaaatc cgaaaacgag tccatcatgc ggaaatcatt acaacacaaa
     7441 aatccttccg tacgctattg ggcttcacac tttttaacaa agtcattggg tacagcagca
     7501 cttgaggata ttatgaaagc tcaggaaatt gaatcggtgt ggtgggttgc aaacgagatg
     7561 gaagataata tctatatcct tgcacgcaaa aaatttaatg acgaaatggt tgaacaactg
     7621 ggttcattca ggtttgttat tgataaaaag gataagcagg tacaataagt ttaactataa
     7681 ttttggcgat agtttccaga tatactttcc aacaatagca ttaagcggga tagtaccaaa
     7741 caatctgctg tcggtaggga tgtcccaatt atcacaaatc acaaatatat gcccattggg
     7801 gacagttatc gcagcaaggt tatctcgcgc agtgaatcgt ggcgggaacg tcctggtatc
     7861 tttttgcaca atcttccagt gtatatgcaa aggaatgccg ttaacatata tcctcttatt
     7921 aataatctca atggtatcac cagattttgc aataactcta ccaacctgtt tacctgaaag
     7981 aggacttttg taaagaacta tcgaacctga atcaactttt cttgttttat aaaaccatgc
     8041 ataatcatta ttgtgtaatg ccggttccat aatgacatta ttaattttta caggaataaa
     8101 aaacaaccgg ataagtataa atccaaaaat taaacctgca aaaataaaca atacctttgc
     8161 aaaaatatta aacgaatctt ttgtctccat atgtattgct acttttttat cttatttcag
     8221 catttcaagt gcttttttaa actgaggtat ggatttttct attacctttt caggcacgca
     8281 aaaggctaat ctaaaatagc caggcccgcc aaatccaacc ccgggcactg caagtatatt
     8341 aaaatttaaa agatgcttta caaatataac atcatcttct atgggcgatt tgcaaaaaac
     8401 ataaaaagcg cctttagggg taataaaact atacccggca tctttgagac cattgcaaaa
     8461 catatcccgc cgaactgcat atgcattgac atccaccctg tcaaaggcaa ttctttcaac
     8521 tatccgctgc ataagagcag gagcattgac aaatcccaat attctgttac acagtattag
     8581 tcctgccata ataatatctg cttcgggcat tgatggattt atcgcaatgt atccaatgcg
     8641 ttcaccagga agtgacatcg tttttgaaaa agaagaaaca accattgaat agttataatg
     8701 tgataaaata ctgggaactg tgctaccatc atatatcagt tctcggtatg gctcatctga
     8761 taacaggtaa ataggcttcc catatttatt tgaatagcgt tccagcagtg atgataatcg
     8821 tgtaatatca tcatgtgaat agataacacc ggtagggttg ttgggagaat ttattaaaac
     8881 tgctcttgtt ttatcattga ttgctttttc cagggcatcg acatcaagtg aaaaatcagc
     8941 attggtggat actaatgtta ttactccatt atggttggaa atataaaatt gatattctgc
     9001 aaaacagggt tttggtacta tgacttcttc accaggattt aatatggttt tcaaagctac
     9061 attgagccct cctgctgcac cgcatgtcat aataacatgt ttcccctgaa tatcaacatg
     9121 atgatctttt gccacctttt tagcaatagc atctcgtgtt gttgtaaatc ctgcatttgg
     9181 catgtatcca tgtgctttag ggtcaggttt tgatgctaat gcttttaacg ttgtataaaa
     9241 tgattcaggt tgtggaacat caggatcgcc taaactgaaa tcataaacat tctcttcacc
     9301 aaactgcgct ttaagttttg ctccttcctc aaacatcttg cgtatccatg aagagtgaat
     9361 catcgcttca gaaatatgtg tagcaactga catggcaacc tcccgttaaa atagcaggat
     9421 agttattggg gaactatcgt gcaataataa taaattcttc ttttttatta tcatactatt
     9481 ttcaaaagat ttttatcaag tctttttcat ataatattta ccatttttat accatcccat
     9541 agtaaaagca acctcttaac attgtttact tttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnggatatg tatttgtgtt tgttaaaaat ggggctgtaa aaaattaact
     9721 tttagacagc cccttacatg atacaatgta atgtatagca acttttcaag ctttgagaag
     9781 tgattccata gtatcaagta attcatatag ttcttgcggc aggtgattgc caaacttagg
     9841 gtaatgatta ttccttacat catctacctc acgcagccac ccttctctgt caacttttaa
     9901 caatatttcc atatcctgat ttgatacaga agccggctta tcaatggcat caggagcggg
     9961 catatagcca acaggagtat caactgcttt accactacca tcacatcgtt caaatatcca
    10021 ttttagcact ctgctatttt caccatatcc gggccatagc catttgccat catcggtttt
    10081 tctgaaccag ttaacaaaaa atatctttgg caacttttca gatgttgttt tcttttctat
    10141 gtttatccag tgtttaaagt aatctcccat atggtatcca caaaacggaa gcattgcaaa
    10201 tgggtccctg cgcacctgtc caatggtaag atccaatgcc gctgcagtta cttcagaacc
    10261 aactatcgaa cctaaaaaca caccgtgcag ccaattttta gattccacca ccaatggtat
    10321 tgtttgtgcg cgtctaccgc cgaataatat tgcactgatt ggtaccccgt ctggatcttc
    10381 ccattctttt gcgatggatg ggcattggtt tgctggggtg gtaaatcgtg aattaggatg
    10441 agcagcaggt ttttgatctt tattgttttt atcatatatc cattcattcc caagccagtc
    10501 aattagtttg cccccgggtt catatcctat accttcccac cacacattca aatcttcagt
    10561 taatgcaaca ttggtaaata ttgtattttt ttctattgtc ttcatagcat tatagtttga
    10621 ttcttcagaa gttcctggtg caacaccaaa aaatcctgct tcaggattga tcgcatacaa
    10681 ctttccatca gacccaaatt taagccatgc aatatcatcc ccaacggttt ctaccgtcca
    10741 cccgggaatg gttggtataa gcatagcaag attggtttta ccacatgccg atgggaatgc
    10801 tcctgctata tattttgcct ttccttcagg attggtaatt ttcaatatca gcatatgctc
    10861 ggcaagccag ccttcatcgt gtgccatgac cgaagcaata cgtagagcca aacacttttt
    10921 ccctaacaac gcgttgccac cataccctga accgtaagac cagatctcac gtgtttcagg
    10981 aaaatgcgag atatactttt tttctaatgg agcgcatggc catttaccat tatcactttc
    11041 tccctttgtt aatggtttcc caactgaatg ccagcacgga acaaactccc cttcagttcc
    11101 cagaacatca agcaccttgc tgccaacacg cgtcataata tgcatattaa taacgacata
    11161 tggcgaatca gtaatctcca caccaatttt tgcaattgat gagccaacgg gtcccattga
    11221 aaacgggatt acatacatga ttcttccacg catacatcca cggtaaagat cagtcattgt
    11281 ttgttttaat tctaccggat caatccagtt attagttgga ccagcagctt cctgcgtctt
    11341 tgacgcaatg aacgtcctgt tttccaccct ggctacatcg cttggatcag atctaaaaag
    11401 taaacagttt gcccgttttt tttgatttaa ctgtactgcc aaaccttcat caaccatttg
    11461 ctgggccaac atgtcatatt cgcccttgct tccatcacac acataaacat catctggttt
    11521 catcaaattt gatgcatcct gaatccactt tgttaatgat tggtgtttta actcatgtac
    11581 cttcataatg cttcctttct agtattaaag gattataaaa ctattgtgag taacggttat
    11641 ggtgtacaat ttttttgtca actattacta ttcaaaatag tagcgaccaa tctattaacc
    11701 aaattgtaac aaaattgatt taataataaa aaatagtcta ttttatatga agaataacat
    11761 cgacacgtat ggagtggaat aactttaaaa tatcagatgg gatgttttta aaatcgcata
    11821 gcttattatt ctgagtggca tttttcttta tatcaattag gaaacctatt cctgctgaat
    11881 ctatgtaatt taaatgcgat agatcaataa ttatattttc aggttccttt aaaaatatag
    11941 ccttaatatc ttcttttaaa ttgtaagaag agtacaggtc aagatcaccc tcaattttaa
    12001 gaaaatatcc tgttttatct ttatcaaatg aatataccat acactatcac tttcagagct
    12061 ttttaccaca ctataattat cgtattataa aagataatag ttgaatcaat tcgtccagta
    12121 ccccaataaa tagcctgcta tataatgtgt ttatattatc actattagaa tatatagcaa
    12181 tatcctcaat aaaatcacat aaaaaactac tgatataaaa taactacaca gtaactatcg
    12241 caaacataca atatattcgt aggtaaacat atcccccata aactgtcgca agatttcatg
    12301 ctcatccctg ctattgactg tactgttaag aataaatcgc aattgag
//
LOCUS       DAOOJH010000068        12143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4822_length_12143_cov_9.651721, whole genome shotgun sequence.
ACCESSION   DAOOJH010000068 DAOOJH010000000
VERSION     DAOOJH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_12143_cov_9.651721"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..905
                     /locus_tag="PLJ75_09025"
     CDS             363..905
                     /locus_tag="PLJ75_09025"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HPD05394.1"
                     /translation="MESKKIFSRTIDIVSYQDGPDSVYIKGEIKDTRLCQNYLVTGEK
                     QNPGVLHNMIITMRVSGSNLVISDIQVTMKQYPREECAEANYNIQKLNGISITKGFTK
                     AVKDAIGGTKGCSHVTNCILAMGPAAVQSFWAMKAQQPVSKEFDSGGSMSQYLIDTCH
                     VWRKDGPMVKNLAEIINKLK"
     gene            complement(913..1917)
                     /gene="thiL"
                     /locus_tag="PLJ75_09030"
     CDS             complement(913..1917)
                     /gene="thiL"
                     /locus_tag="PLJ75_09030"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPD05395.1"
                     /translation="MKLNDIGEFGFIKRITPDTIYDDRNVLRAIGDDAAVFTVSHDEC
                     IVLTTDLLVERIHFIKETIAGYQLGYKSLAVNLSDIAAMGAKPQHAFVSIGIPIETPL
                     AYLDELYRGMKQLAQLYSVNILGGDTTSSKTDLIINVAVTGVAKKNSILYRNTAKAGD
                     AIYCTGFLGDSRAGLDFILQNKNPQHDYEKKLLHAHTLPRPHVEEGLFLSNTGAVHSC
                     IDVSDGLSSDLLHIAEESNVGFILDEKSIPISPQLQQYCDDNALNATDYALSGGEDYV
                     LVFTVDENASLQLEKNFYNTFNRAIYKIGIITPDKKYVMRKIDGSLINIKAGGWDHFV
                     "
     gene            complement(1904..2554)
                     /gene="thiE"
                     /locus_tag="PLJ75_09035"
     CDS             complement(1904..2554)
                     /gene="thiE"
                     /locus_tag="PLJ75_09035"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPD05396.1"
                     /translation="MKRFTSSLYLVTDRGLAKKPIEILVEEAIRGGVDCVQLREKNIA
                     TKHFVALAKTVKNITIRYNVPLIINDRIDVALAVDADGIHLGQDDMPLTNARKLLPHK
                     MIIGITVSTINEAIEAQKGGADYIGASPVFATPTKTDTAAPLGLEGLQEIKDAVTIPV
                     VAIGGINLHNAEDVLLHGADCLAVVSAIVCADNPCQAAANLAAIIKKLRKSNHETE"
     gene            complement(2571..3371)
                     /gene="thiM"
                     /locus_tag="PLJ75_09040"
     CDS             complement(2571..3371)
                     /gene="thiM"
                     /locus_tag="PLJ75_09040"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPD05397.1"
                     /translation="MNLHEYAGETLKKIRAQKPLIHNITNYVVMNYTANALLACGASP
                     VMAHAYNEVRDMVGIADALVINIGTLEEEWIRSMIIAASEANKKGIPIILDPVGAGAT
                     KFRTTAALRILRAAKISVIRGNASEILALTDSSSQTKGVDSQHGVDEATDVAVELSLR
                     YNAVVAVTGVTDVVTDGNAAIKVSNGHSMMRYVTGTGCAATALIGTFCAVESDHLKSA
                     ASALAYFGIAGELAAKEAMTPGSFMIALLDKLYAITPVELEQYAKFAI"
     assembly_gap    3458..3557
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3558..4854)
                     /gene="thiC"
                     /locus_tag="PLJ75_09045"
     CDS             complement(<3558..4854)
                     /gene="thiC"
                     /locus_tag="PLJ75_09045"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HPD05398.1"
                     /translation="MTQLDYAKQGIITDAMKQAAAFEGMKPEELLQLMAKGWAVIPKN
                     IKRNFNVRAIGKGLLTKVNANIGTSTDHGDIDEECKKLETAVYYGADSVMDLSTGGNL
                     TAIRTMILQNANVMVGAVPIYRVAAELAANNRSILHMSHNQLFEAIEEQCQNGIDYMT
                     VHCGVTQKSMELASRQRRVMGIVSRGGSLLSAWMKHHKRENPLYEDYEKLCAIAHKYD
                     VTLSLGDGLRPGATADATDAAQIEELILLGDLVQRARDAGVQVIVEGPGHVPMEQIAA
                     NIVLQKRICDNAPFYVLGPLTTDIAPGYDHIVGAIGGAIAAYAGADFLCYVTPAEHLC
                     LPSVDDVRLGVIGTRIAAHSADIAKGIPKALQQDLEMSQFRKSLNWEGMISRAIDPDM
                     AKQRRSNSEDYNEKVCTMCGKLCAITIHNECELLHHDGDV"
     regulatory      complement(4925..5026)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5089..5430)
                     /locus_tag="PLJ75_09050"
     CDS             complement(5089..5430)
                     /locus_tag="PLJ75_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPD05399.1"
                     /translation="MMKTKTLQDTLQFAQMVAGKHGWVLHPDKEFLDILVEGLTTNYN
                     RYGYFSCPCRDADGIREKDKDIICPCEYCAPDIQEYGSCYCGLYNDPLFIKNGGYPES
                     IPERRQFKNFI"
     gene            complement(5437..5739)
                     /locus_tag="PLJ75_09055"
     CDS             complement(5437..5739)
                     /locus_tag="PLJ75_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPD05400.1"
                     /translation="MIENITFTYKDGLLSPGDITVYALSTCGHCKRALKFLDDNSVKY
                     KFVYVDLLPSEEKNNIKDYLAKKSNMYVAFPFLVIDDTTFFTGFIEEEWRRLLKME"
     gene            complement(5753..7216)
                     /locus_tag="PLJ75_09060"
     CDS             complement(5753..7216)
                     /locus_tag="PLJ75_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05401.1"
                     /translation="MKCKKCGNKIPLLIEICPYCGYEFHATHHKNVHNDNANTAEDAV
                     HDDRADDTQKILNDIKNIKNYESNNIGSIAILIISILLFFSIGYAKHPLKDTIIVIIV
                     LLLHEFGHLLGMMIFKYKNIKMFFIPFFGAAVSGLKLNKDGFNEAIISLLGPMPGILI
                     AIACMFFFKNNDNLNQIAASLIFINGFNLLPIYPLDGGRIMDIIFQRSMVAQLLFRIV
                     TSLALIYLAVFSHTLLLAFVGIIIILSIPDNYRTNKAVKALKADIPATVSIENEDKVL
                     QDNIGIIYATVKAIFPKVKGKRYANIILAVWDKLTFKAPNFIKSLIITIIYGMSIAML
                     VVSTALFYFNYSRSHSRLESSIVSMVDDSGNHYQLQQIKYHNKLLYEIQVHNDLYHGF
                     CKEYSPITNKVVTEGYWYKGKWDKEWKFYNSKGEVYRIDFFDRGTFKFRSEFINGTLQ
                     IIKFEELNNNDKMKFKNHEQAKPLGPNNVHVSTDIVQ"
     gene            complement(7288..7470)
                     /locus_tag="PLJ75_09065"
     CDS             complement(7288..7470)
                     /locus_tag="PLJ75_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05402.1"
                     /translation="MDELILYLISEYHTAVMHTLTIISVMAVWVVICGCIRFGIAVYR
                     RAKGIYPPAESLREGH"
     gene            7870..8556
                     /locus_tag="PLJ75_09070"
     CDS             7870..8556
                     /locus_tag="PLJ75_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPD05403.1"
                     /translation="MSIVDNYHYIQSEIKNIALRCNRNPDDITLIAVSKTFPPEIIQE
                     AIDNGITLFGENRVQEAKAKIPQLKGNFTFHLVGHLQSNKAKDAVKLFDCIHSIDRLS
                     TAIEVDKEAKKIDTIQKILIEVNTSGEESKFGVVPSQAIALCKSILDLHNIQCHGFMT
                     VGPLTDDRSAIRNAFALLRTLRDEINIKLGTSLHELSMGMSGDYDIAIEEGSTMLRIG
                     TAIFGKRISQ"
     gene            8553..9356
                     /gene="proC"
                     /locus_tag="PLJ75_09075"
     CDS             8553..9356
                     /gene="proC"
                     /locus_tag="PLJ75_09075"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPD05404.1"
                     /translation="MNIQKTTIGIIGLGNMGGAIGTGLSRIIDPKNIIGYDISSQAMT
                     SLTSTLSISTASSIQELSHKSDVIIIAVKPAIVASVASELKSTTKNKLIISIAAGITL
                     HKLSEWLGDSFRIIRCMPNMPALVGEGMTVLSTTSANNDDMEIAQQIFGALGRVMVVS
                     EQLMDAVTAVSGSGPAYVFTLIQAMADAGVKSGLTRDNAVTLAAQTVLGSATMVLQGE
                     EPIVLRGKVASPAGTTIDAIHVLEKHGFSGIIMDAIEAAFLKSRTLGGK"
     gene            9357..10313
                     /locus_tag="PLJ75_09080"
     CDS             9357..10313
                     /locus_tag="PLJ75_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HPD05405.1"
                     /translation="MLTNYHVWQIKKAIKVLIPVMVFIFIAGNIICYFVKEPVKIPDD
                     AVYKKNGFLLFKDADAYITILKKYPYNPGVTLTIYTMREGESYWDVIQRYRISIDTFI
                     GANPHLDSLLAKAGTQVVIPHQDGLLIPCRHFYDAWLAKLSINGTTNIKGHYLHHPFE
                     LLAMDDIRFAFIKHAKPIAVNDKIEKLYTLRQKFQSPINGRYTSMYGDRVDPLEHGMA
                     FHNGIDICAPMGTPINPVRNGFVNFTGWLDGYGFTIIIQHDEGYESWYGHCQKIFAQK
                     GDFVTNNSVIGTVGSTGRSTGPHLHFMLLQHGKMLNPIMFIW"
     gene            complement(10324..11055)
                     /locus_tag="PLJ75_09085"
     CDS             complement(10324..11055)
                     /locus_tag="PLJ75_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPD05406.1"
                     /translation="MRAIVLAAGTGSRLGEMTKNKTKGMVRVNGKPLIDYLFEFFELD
                     FFDEIIVVGGFAFDELNSHLSGKNIDNIKILENKDYLKGNIFTLITALKTFAHDSFLI
                     TNVDHIYPKSMFQRMKQSLYKITAMCDFDRTLGLDDMKVKLLADKKTIAHISKQLTDF
                     DCGYIGMTYVDKSMEIAYRDAAYKAIERYGEKAVVENIVQILAENPKVAPVICDLSGF
                     GWYEVDDENDLIKAEKGLLQNQNFN"
     gene            complement(11043..11681)
                     /locus_tag="PLJ75_09090"
     CDS             complement(11043..11681)
                     /locus_tag="PLJ75_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPD05407.1"
                     /translation="MKTRALLFDFDGTLVDTMEGFADIAGEVIHQFHPAISYEEARRR
                     YLETSGVPFFQQLEIILPGDPTNSKKAAIFEETKKEGFFRSTFSEEVRFVITQLRGKG
                     ILVGVSSNNFQYLIEQFIEREGLQFDIILGFKDGFEKGKHHFEYVLQHFQLKKDELTF
                     VGDSLKDAEKAISNDISFIGLCGTFTKEQFLDKYPNIITINSLKELLDICER"
     gene            complement(11726..11857)
                     /locus_tag="PLJ75_09095"
     CDS             complement(11726..11857)
                     /locus_tag="PLJ75_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05408.1"
                     /translation="MHGAFGCRRMIGQPAAHNHYTASLSGQAGNTVVRLQLIFYIDK"
BASE COUNT         3744 a         2429 c         2041 g         3829 t
ORIGIN      
        1 aattattaag aatacattta ctggtaaaaa tgatgaataa ttaaatacga catatatagt
       61 accatattaa aaattattac catacattgt caaccaatta tttatatata acatttttat
      121 tcatttattg atatataact acaaaacata tttatatata tataataatt tttataggaa
      181 aagcaactta tagcttccaa taactttaaa tttgtttctc acattatagt agcgatttcg
      241 tcgtctgtat tgagtcatca atggacatta tgcaataact ttccatggaa ttttaattga
      301 aaaattattt gttttttatt acatagcagt aagcttatta cttattatag gagaaagtta
      361 ctatggaaag caaaaaaatt ttttctagaa ctattgatat agttagttat caagatggac
      421 ctgattcagt ttatattaaa ggtgaaatca aagacacacg cctgtgccaa aattatctgg
      481 taactggaga aaagcaaaac ccgggtgtac tgcacaacat gatcattaca atgcgtgtca
      541 gcggcagcaa cctggtgatc agcgatatac aggtaacaat gaaacagtat ccacgcgagg
      601 aatgtgcaga agcaaattat aatattcaaa aattaaacgg gatttctata acaaagggat
      661 ttacaaaggc tgtaaaagat gctattggcg gtacaaaagg ttgttcgcat gtaacaaatt
      721 gtattctggc tatggggcct gccgctgttc agagtttctg ggctatgaaa gctcaacaac
      781 cagtttcaaa agaatttgat agcggcggct caatgtctca atatttgata gatacctgcc
      841 atgtatggcg gaaagatggt cctatggtaa aaaatttagc tgagataatc aataaattaa
      901 aataatattt tattatacaa agtggtccca tccacctgct ttgatattaa ttagtgaacc
      961 atctattttt ctcattacat attttttatc tggtgttatt attcctattt tgtagattgc
     1021 tctattaaat gtattataaa aatttttttc taattgcaat gaagcatttt catcaacagt
     1081 gaaaaccagc acataatctt cgccgcccga caatgcatag tcagtagcgt ttaatgcatt
     1141 atcatcgcaa tactgttgca attggggtga aattggtatt gatttttcgt ccagtataaa
     1201 tcccacatta ctttcctctg caatgtgcaa tagatcagag cttaacccat cacttacatc
     1261 tatacaggaa tggactgcac ccgtattgct taaaaacaaa ccctcttcaa catgaggtcg
     1321 cggtaatgta tgagcatgga gcaacttttt ttcatagtca tgctgtggat ttttattttg
     1381 taaaataaaa tccaatcccg ccctgctatc gcccagaaag cctgtacaat agatagcatc
     1441 accagctttt gcggtattac gatataatat gctatttttt tttgcaactc ctgtaactgc
     1501 aacattaatg attaaatcag tttttgaaga tgttgtgtcc ccacccagta tattgacact
     1561 atacagttga gctaattgtt tcatccccct gtatagttca tccagatacg caagcggtgt
     1621 ttctattggg attcctatgc ttacaaaggc atgctgaggt ttagctccca tagctgcaat
     1681 atcactcagg ttaaccgcca gcgatttgta gcccagctgg taacccgcaa tggtttcttt
     1741 tataaagtga attctttcca caagcaaatc agtggtaagc actatacatt catcgtgact
     1801 cacagtaaat acggctgcat cgtcacctat agcgcgcaat acatttctat catcataaat
     1861 ggtgtcgggg gttatccgct taataaaacc aaattcaccg atatcattca gtttcatgat
     1921 ttgatttcct taactttttt atgattgctg caagattggc tgctgcctgg cagggattat
     1981 ccgcacaaac aatggctgaa acaacagcta aacagtcagc tccatggagt aaaacatctt
     2041 cagcattgtg aagatttata ccgccaatgg caacaacggg tatcgttaca gcatctttta
     2101 tttcctgcaa tccttccaat cctaacggcg ctgcggtatc agttttagtt ggggtggcaa
     2161 acacagggct tgcacctata taatcagctc cacctttttg tgcttcaatt gcctcattaa
     2221 ttgtagaaac cgttatgcca atgatcattt tatgaggaag caattttcga gcatttgtca
     2281 atggcatgtc atcctgccct aaatggatac catcagcatc tactgccagc gccacatcta
     2341 tacgatcatt aattattaaa ggaacattat atctgatagt aatattttta acggtttttg
     2401 ccagtgcaac aaaatgtttt gtagcaatat ttttttcccg caactgtacg caatccactc
     2461 ctccccgtat agcttcttca acaagtattt caataggctt ttttgccaat cctctgtcgg
     2521 tcactaaata taatgaagat gtaaaacgtt tcattgtaat tacctcatac ttatatagca
     2581 aattttgcat attgctccaa ctctacagga gttattgcat acagtttatc cagtaaagca
     2641 atcataaagc tgcccggcgt cattgcttct tttgctgcca gttcaccagc aataccaaaa
     2701 tacgccagtg ccgatgcagc cgattttaaa tggtcacttt caactgcaca aaatgttcca
     2761 atcaatgctg tcgcggcaca gcccgttcct gttacataac gcatcattga atggccattg
     2821 cttactttta ttgctgcatt tccatcagtg acaacatcag taactcctgt tactgcaaca
     2881 actgcgttgt agcgtaacga tagttccact gcaacatcag ttgcttcatc aactccatgt
     2941 tgtgaatcaa caccttttgt ttgtgagcta ctatcggtca aagctaaaat ctcagaagca
     3001 ttaccacgaa taacagaaat tttagctgcg cgtaaaatcc tcaatgcagc tgtagttcgg
     3061 aattttgttg cacctgctcc aacggggtca agaatgattg ggattccctt tttatttgct
     3121 tcgctggcag caataatcat agaacgaatc cattcttctt caagggtgcc aatattaata
     3181 acaagagcat ctgcaatacc aaccatatca cgtacctcat tataggcatg tgccataacc
     3241 ggggaagcgc cacaagctaa cagggcattt gcagtataat tcataaccac ataattggta
     3301 atattatgaa tgagtggttt ttgtgctctg atttttttta aggtttctcc ggcatattca
     3361 tgtagattca ttgtgttact ccaaaaaaat agtatagtac tgttagcaac tgaagaatct
     3421 atgctttacc ataacgacag attcttcact tcgttcannn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnncaa catctccatc atgatgcaat aattcacatt cattgtgtat
     3601 ggtaatagca cagagcttac cgcacattgt gcataccttt tcattataat cttcactgtt
     3661 actgcgccgt tgttttgcca tatccgggtc aattgccctg cttatcatcc cttcccagtt
     3721 taaggatttt ctgaattgtg acatttccaa atcctgctgc agcgctttgg gtatgccctt
     3781 tgcaatatca gccgaatgag ctgcaatacg cgttccaatt actcccagcc gcacatcatc
     3841 aaccgatgga aggcatagat gctcagcagg ggtaacataa cacaaaaaat cagcgccagc
     3901 ataggcagcg atagctcctc caattgctcc cacaatatga tcatatcccg gggcaatatc
     3961 ggttgtcagc ggtcccagca catagaatgg agcattatca cagattcttt tttgtaacac
     4021 aatgtttgcc gctatctgtt ccattggaac atgccccggt ccctcaacta tcacctgcac
     4081 tcctgcatca cgggctcttt gtacaaggtc acctaataaa ataagttctt ctatctgagc
     4141 agcatctgtt gcatcggctg ttgcacccgg gcgcagtcca tcacctaaac taagcgtgac
     4201 atcatactta tgagctatcg cacataattt ttcatagtcc tcataaagtg gattttccct
     4261 tttatgatgc ttcatccatg ctgaaagcaa tgagcctcca cggcttacta tgcccataac
     4321 ccttctttgc ctggatgcca attccatact tttttgcgta actccacaat ggacagtcat
     4381 gtaatcaata ccattttgac attgctcttc aattgcttca aaaagctgat tgtgcgacat
     4441 gtggagtatt gacctattgt tagcggcaag ctcagcagca acccggtaaa taggtactgc
     4501 accaaccata acatttgcat tttgtagtat cattgtacgt attgctgtta gattgccgcc
     4561 ggtcgaaaga tccataacac tatcagcacc ataatacacc gctgtttcca attttttgca
     4621 ttcttcatca atatcaccat ggtcggttga agttccaatg ttagcattaa ccttggtaag
     4681 caatcccttc cctattgctc gtacattgaa attccgcttt atattttttg gaataacagc
     4741 ccaccccttt gccattaatt gcaaaagttc ttcgggtttc ataccttcaa atgctgctgc
     4801 ctgtttcatg gcgtcagtta tgataccctg ttttgcgtaa tctaattgtg tcatacaggt
     4861 aacccccaca acaaaaaaac cgctcagcga ttgagcggtt tttttacatt aatactatta
     4921 taaaagcatt gtacaaactg ctccctccgc tggcattacc cagttcaggt tcaacgggta
     4981 tactctcagg cctgtgtatc cacacaaacc acccctgctt ttaagaacat catacttagt
     5041 actacaatac tatgaacaca tgaaaaatca agagaaatat tacaataatt aaataaaatt
     5101 tttaaactgg cggcgttcag ggatgctttc cgggtaacca ccatttttta taaatagcgg
     5161 atcattatat aagccacagt aacagctgcc gtattcctgt atatcaggtg cgcagtattc
     5221 gcagggacat ataatatcct tatccttttc tcgtatgccg tcagcgtccc ggcatgggca
     5281 tgaaaaatag ccatatcgat tataatttgt tgttaatcct tcaaccagaa tatcaagaaa
     5341 ttccttatca ggatgcaata cccagccatg cttgccagca accatctgag caaattgcag
     5401 ggtgtcctgt aatgtttttg ttttcatcat tgtttactac tccattttta ataatcgtcg
     5461 ccattcttct tcaataaaac ccgtgaaaaa tgtggtatca tcaataacta aaaacggaaa
     5521 tgcaacatac atgtttgatt tttttgccag atagtccttt atattgttct tttcctctga
     5581 tggcaataga tcaacataaa caaatttata ctttacagaa ttgtcatcaa gaaactttaa
     5641 tgcacgttta caatgtccac aggttgataa cgcatacacg gttatatccc ctggagaaag
     5701 taaaccatcc ttataggtaa aggtaatatt ttcaatcata tcaatcctcc cattactgta
     5761 ctatatcagt acttacatga acattgttag gtccaagggg ttttgcctgt tcatgatttt
     5821 tgaatttcat tttatcgtta ttattgagtt cttcaaattt tataatttgc aaggtaccat
     5881 taataaattc actacgaaat ttgaatgttc ccctgtcaaa aaaatcaatc ctgtaaactt
     5941 cacctttact attataaaat ttccactctt tatcccattt acccttatac caataaccct
     6001 cggttacaac tttatttgtt attggcgaat attccttaca aaacccatga taaagatcat
     6061 tatgtacctg aatttcgtac agtaatttgt tgtgatactt aatctgctgc aattgataat
     6121 gattgccgct atcatcaacc atagaaacaa tactgctttc caatcggcta tgactacggc
     6181 tatagttaaa ataaaataat gcagtggata ctactaacat tgcaattgac ataccatata
     6241 taatagtaat aatcaaactt ttaataaaat ttggtgcttt aaaagttagc ttatcccata
     6301 ccgcaagtat tatattggca tatcgcttgc ccttaacctt ggggaaaata gctttaactg
     6361 ttgcatatat tataccaatg ttgtcttgca acactttatc ctcattttca atggacaccg
     6421 tagctggtat atcagccttc aaagctttta ctgctttatt ggtacgatag ttatcgggaa
     6481 tcgatagaat aatgataatc ccaacaaatg ccaataacaa tgtatgggaa aaaactgcta
     6541 aataaataag tgccagcgat gttactattc taaataataa ttgagctacc attgaccttt
     6601 gaaaaatgat atccattatc ctgccaccat caagagggta aataggcagt aagttaaacc
     6661 cgttaataaa tataagcgat gctgctattt gattaagatt atcattattt ttaaaaaaaa
     6721 acatacatgc tatggcaatc aatatgccag gcatgggacc aagtaatgat atgatagctt
     6781 cattaaaacc atctttattt aattttaaac cacttacagc tgcaccaaaa aaaggtataa
     6841 agaacatctt tatattttta tatttaaaaa tcatcatgcc aagtaaatgt ccaaactcgt
     6901 gtagcaacaa aacaataata acgataatgg tatcttttaa aggatgtttg gcataaccaa
     6961 tagaaaaaaa caaaaggatg gaaatgatta aaatcgcaat actacctatg ttattgcttt
     7021 cataattttt aatattttta atatcattta aaattttttg agtatcatct gctctatcat
     7081 catgcacagc atcttcagct gtatttgcat tgtcattgtg cacattttta tgatgagttg
     7141 catggaattc atatccacaa taagggcata tttcaatcaa caatggtatc ttgtttccgc
     7201 actttttaca tttcataatg tttttgcctt ataaaaaaag cgatgacagt gcatcgcttt
     7261 ttataaatta gtataaaaac tattgaatta atgcccttct cgcaagcttt cagcgggtgg
     7321 ataaatgccc tttgcccttc tataaacggc tataccaaaa cggatacatc cacagatgac
     7381 cacccacact gccataacag aaatgatagt aagagtatgc attaccgctg tatgatattc
     7441 actaatcaga tatagaatta attcatccat cactcacctc aaaaatgaaa gtttattatg
     7501 ataagtacaa taataacttt taaattttca tttaatattg tcaatacaaa attttataat
     7561 tcataaaatt ttaccggaaa atgttgtttt tataaaattg aaagtgcgtt gcttttcatt
     7621 aaaggcttta catttttttt gtaaaatgat agtgtacaat cactattttc tttttgatga
     7681 aggtattgca ttttgttaaa aagtgtttga ctttataaat taccatacaa ataattaata
     7741 ttaatgagta aatagccgtt tttttatgat tgtatcatca ggtgcagcaa aaatatcata
     7801 caaaacaaag catcaatcat tgaaatagct atagctacta tttaaaaaag gttttgccaa
     7861 tttaacgcta tgagcatcgt tgacaactat cattacatcc agtctgaaat aaaaaatatt
     7921 gcccttcggt gcaacaggaa ccctgatgat attacactta tagctgtaag caaaacattc
     7981 ccccctgaaa ttatccagga agctatcgat aatggcatta ctttatttgg cgaaaacagg
     8041 gtgcaggaag caaaagcaaa gattccgcaa ttaaagggta attttacatt tcaccttgtt
     8101 ggacatcttc agtcaaacaa ggcaaaagac gccgtaaaac tgtttgattg cattcattcc
     8161 attgacaggc tatcaaccgc aattgaggtt gacaaagaag caaaaaagat tgatacaatc
     8221 cagaagattt taattgaggt taacacctcg ggtgaagaat caaaatttgg cgttgttccg
     8281 tcacaagcca ttgcattatg caaatctatt ctggatttac acaatatcca atgtcatggt
     8341 ttcatgacgg taggtccttt gaccgatgac cgttcagcaa tacggaatgc atttgcatta
     8401 ttgagaacat tacgggatga aataaatata aagcttggaa cttcattaca tgagctatcg
     8461 atggggatgt caggtgatta cgatatagca attgaagaag gctctaccat gcttagaatt
     8521 ggaacagcaa tttttggtaa aaggatatcg caatgaatat acaaaaaaca acaatcggta
     8581 tcattggtct ggggaatatg ggcggtgcta tcggtacagg gctttccaga atcattgatc
     8641 caaaaaatat tattggttac gatatcagca gtcaggcaat gacttcactt accagcacac
     8701 tatcaataag tactgccagc tcaattcagg agctatcgca caaaagtgac gttatcatca
     8761 ttgccgtaaa acctgcaatt gttgcgtcgg tagccagcga gctaaagagt acaaccaaaa
     8821 acaagcttat catttcaata gctgccggta tcacacttca caagctttcc gaatggctgg
     8881 gcgatagttt ccgtataatc aggtgcatgc ccaatatgcc tgcccttgtt ggcgaaggga
     8941 tgaccgtact atcaacgaca tccgccaata acgatgatat ggagattgca cagcaaatct
     9001 ttggagcgct gggcagagtc atggtagttt ccgagcagtt gatggatgct gttactgctg
     9061 tttcgggaag cggtccggca tatgttttca cattgataca ggcaatggcg gatgcggggg
     9121 ttaaatcagg acttacacgc gataacgccg tgacactggc agcccaaacg gtgttaggtt
     9181 cagctacgat ggtattgcag ggcgaagagc caattgtttt acgggggaag gtagcttccc
     9241 cggccggcac taccattgat gcgatacatg ttcttgaaaa acatggattt tccggaatta
     9301 taatggatgc cattgaagct gcatttctga aatccagaac gcttggaggt aagtaaatgc
     9361 ttactaacta tcatgtatgg cagataaaaa aagctataaa agtacttatc cctgttatgg
     9421 tttttatttt tattgctggc aatattatat gctattttgt aaaagaacca gtaaaaatac
     9481 ccgatgatgc ggtttataaa aaaaatggct ttttactctt taaagatgca gacgcataca
     9541 tcacaattct aaaaaaatac ccctataatc ccggtgtaac attgaccatt tatacaatgc
     9601 gtgaaggtga atcatactgg gatgtcatcc agcgctatag aataagcatt gatacattta
     9661 ttggtgccaa cccgcatctg gattcattac tggcaaaggc agggacacag gttgttattc
     9721 ctcatcagga tggactgctc atcccctgca ggcatttcta tgatgcatgg ctggcaaaac
     9781 tgtctataaa tggtacaaca aacattaagg ggcactatct acaccacccc tttgagcttc
     9841 tggcaatgga tgatatacgt tttgccttta taaaacatgc gaagccaatt gctgttaatg
     9901 ataaaataga aaaattatat accttacggc aaaaattcca atcccccatc aatggccgat
     9961 acacatcaat gtacggcgat agggttgacc cattagagca tggcatggca ttccacaatg
    10021 gcattgatat ctgcgcaccg atgggaacac caataaaccc tgtaaggaat ggatttgtaa
    10081 attttaccgg ctggcttgat ggttatggtt ttaccatcat cattcaacat gatgaaggat
    10141 acgaaagctg gtacgggcac tgccaaaaaa tatttgcgca aaaaggtgat tttgtgacaa
    10201 acaattcagt cattggtacg gttggctcta caggacggtc caccggtcct cacctgcatt
    10261 ttatgctgtt acagcatggt aaaatgctca atccaataat gtttatatgg tagatagtta
    10321 ctatcaatta aaattttgat tttgtaaaag tcctttttca gcttttatga gatcattttc
    10381 gtcatccacc tcgtaccagc caaaacctga taaatcacag attacaggag caacttttgg
    10441 attttcagcc aatatctgga caatattttc aaccacggct ttttcgccat accgctcaat
    10501 tgctttgtac gcagcatctc tataagctat ttccatactt ttatcaacat aggtcatacc
    10561 aatataaccg caatcaaaat cagtaagctg tttactgatg tgggcaatag tttttttgtc
    10621 agcaagcagc tttactttca tgtcatccaa tcccaatgta cgatcaaaat cacacattgc
    10681 agtaatttta taaagcgatt gtttcatccg ttgaaacatt gattttgggt agatgtgatc
    10741 aacattggtg attaaaaagg aatcatgagc aaatgttttt aatgcagtaa tcaatgtaaa
    10801 gatatttcct ttaaggtaat ctttattttc aagaattttt atattatcaa tattttttcc
    10861 tgaaaggtgg ctattaagtt catcaaatgc aaaaccgcct acaactataa tttcatcaaa
    10921 aaaatccaat tcaaaaaatt caaatagata atctataagc ggtttaccat tgacacgcac
    10981 catgcctttg gttttatttt ttgtcatctc gcccagcctg ctgccagttc ctgctgccag
    11041 aactatcgct cgcatatatc taacaactcc tttaatgaat tgatggtaat tatattggga
    11101 tacttatcta aaaattgctc ctttgtaaat gtgccgcata aacctataaa tgaaatgtca
    11161 ttgcttatag ccttttctgc atctttaagg gaatctccaa caaatgtcaa ttcatctttt
    11221 tttaattgaa aatgttgcag tacatattca aaatggtgct tacccttttc aaatccatcc
    11281 ttaaatccaa ggattatatc aaattgcagt ccttctcttt ctataaactg ttcaataaga
    11341 tactgaaaat tatttgatga aacaccaacc agtatacctt ttccccgtaa ttgtgtaata
    11401 acaaaccgca cttcctcaga aaatgttgat ctgaaaaagc cctctttctt tgtttcctca
    11461 aatatagctg cttttttgct gtttgttgga tcacccggta aaattatttc cagttgttga
    11521 aaaaatggaa cgcctgaggt ttctaaatat ctacgtcgcg cctcttcata ggaaattgca
    11581 ggatggaatt ggtgtattac ttcaccggca atatcagcaa atccttccat agtatcaaca
    11641 agtgttccat caaaatcaaa aagcaatgct cttgttttca tacatgtatc accattcaag
    11701 ttttcatcaa tcttatggat aatcgctact tgtcaatata aaaaattaac tgcaggcgca
    11761 ccacggtatt tcccgcctgt cctgacaacg aagctgtgta atggttgtgt gcggcaggct
    11821 gaccaatcat gcgcctgcat ccaaatgcgc cgtgcatttc acaatactta accccccttg
    11881 tgttcccccc ctttataaaa ggtgggaatt gtgtggatgg tttattagca caaaataggt
    11941 ttgtattctt cataattgtt tttgcagctt aatcccgtag taaccacatt atgcttactt
    12001 gataagttct gcattcttca tggtaaaatt tcttcaaatc ttgtagggat ggaattgata
    12061 tttaataaat ggggttggaa tttttttttc atattttctt ttgttgggcg acaaaagttt
    12121 tatcgtttcg taaggggata ttt
//
LOCUS       DAOOJH010000069        12088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4845_length_12088_cov_8.720020, whole genome shotgun sequence.
ACCESSION   DAOOJH010000069 DAOOJH010000000
VERSION     DAOOJH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4845_length_12088_cov_8.720020"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1481
                     /locus_tag="PLJ75_09100"
     CDS             132..1481
                     /locus_tag="PLJ75_09100"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HPD05409.1"
                     /translation="METILKKIEKLMVQHDAISPELIQKKNVKLGLRNPDGSGVVAGI
                     TSKGTVIGYEKIPSGDDKVTVKPVEGKLYYCGYDACELAEKIQEEGRYGFDEITYLLL
                     TGELPDKKDLEQFSEVIADRRPLTKFERSIIMAEDVNNNQMYALHSVISHLNRCDPDP
                     DSTDIKDVIRQCINLIAKVPVIVAYNYNVMKFRKGGDLIICKPNAAWSTAENFLYLLH
                     GDGERPNEYEAKLFDLALMLHAEHGGGNNSTFTVRTVTSSGANTYMALCAGIASLSGH
                     LHGGANESVMKMMKDIKQNVKDWDDDDEIEHYLKLILQKKAYDRSGKIYGIGHAVYTK
                     SDPRAVIFRKKAEEFAKMKKNLEEFTLFTKVADIGTQLLLGDKSKIVSPNVDFYSGLI
                     YKMMGIPMEIFTPIFAMARFAGWSAHRIEQIIHNKIIRPAYASSIDEYKNYIPLNER"
     gene            1606..3099
                     /locus_tag="PLJ75_09105"
     CDS             1606..3099
                     /locus_tag="PLJ75_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPD05410.1"
                     /translation="MKYDVIVIGAGNAGLTAAATLAKKGVKTLLIEKHNIPGGCATSF
                     CRGRFEFEVSLHQLSGMGTPERPGPLRSILDSVGVLDTLEFVQMDKLYRVVVPGLIDI
                     TLPADKEGIVTALQAKFPEEKDAIKKFFDFVWQFFMEVIGAFYLNDPDINQKKYPLYY
                     KYALKDTQSVLNEYFTNPMLKAVITPYWTYMGLPPSKLCFSDMAALLFSYAEFKPYHI
                     KGGSQMLSNSLLEKFYSNGGEALFNTEVQKLLVKNNSVYGVKTSDGTEFQSKYVLSNA
                     SAISTFVDMLDDDKVSRRVIEPYKGSSIGTSFFTIYCGLDCEYDKVGINEETNFICNT
                     ANPEYDYELSKVVDTDQSSFIVTCYNVFDNAMVPEGTSQVVLVTMKYADAWLKVPPAQ
                     YYDEKCRCAEAMLNNAERVFPGLVSHIEEMEIATPITHMRYLNHPGGGAYGFDQYVKD
                     STLFISPKPPINGLYCAGSWYGSAGYQPTLTSGHSAAMAIVKDIRNK"
     gene            3109..4257
                     /locus_tag="PLJ75_09110"
     CDS             3109..4257
                     /locus_tag="PLJ75_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HPD05411.1"
                     /translation="MNSEILKQLDGYDNVINEREVLKRYGYDYRLDRGFVRKYLERLH
                     PRYITTKIIDIIEETPSTKTLRLDSVEKYLPPFQAGQYISVNVDIDGIRTSRPYSISS
                     SPDETAYYDITIRRVEGGFVSHYLLDSTKIGNTLQISSPAGNFIHNPIIHSNELVFIA
                     GGSGITPFKSMIQHAVNTGISRDIWLIYGNKNSEELLFHDYFTIVAKKYPTIRYVQVL
                     EEGGASYNGYITAKIIKEVVGDSSSKTFFVCGPQGLYKHILNELQTLQLKPRQIRMEM
                     YGVPTDVFKQSGWPEDVTPDSKFKITIKNKKEFTVAAGEPLLNSMEKNGVIVPSLCRS
                     GECSLCRVKLISGKVYQPEGVKLRQADRRFGYIHSCAAFPITDCEIML"
     gene            4283..5272
                     /locus_tag="PLJ75_09115"
     CDS             4283..5272
                     /locus_tag="PLJ75_09115"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPD05412.1"
                     /translation="MITTKITEMFGVKYPIICGAMMWLCKPKLCAAISNAGGIGNLTA
                     GNYSSEEEFRNAIHETRKLTDKPFFVNVTILPSVRITGEHHKMYLKVCAEEKVAGIEI
                     SGSPIDKACGMEYIEMLKKAGVKLFHKVGAVRHALHAEQVGYDGIYAAGIEEGGHPLS
                     DDVSTMVLTTKIAKTVKLPVITVGGIADGKTMAAAFMLGAEGVMMASRFIATKECEVH
                     DNIKQELVKRQENETTLIMKSLHLQGRALKNDVVNKILDIEQRGGGFEELFPLISGER
                     MSKAWNNGDVNAAPLMVGQSIGLIDDIPSCAELLQRMEKEAETQIKKIYSNIL"
     gene            complement(5345..5905)
                     /locus_tag="PLJ75_09120"
     CDS             complement(5345..5905)
                     /locus_tag="PLJ75_09120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPD05413.1"
                     /translation="MATIVLSGGSITSGYATTQSYVAMLSSDEAIKQHSIINVSHFGD
                     SSFEGIWHFDRVLQHKPDVLIIHFGMDDMYRPVYRSEFKENLVRMVQKARESYIKHII
                     MPTLHLVPNQYDMDAVNVFTRTVREVALDLHCHLATVHIEWMNYLYENNDTLESLLTH
                     DYRYPNEKGHLLIAEAIKKRLIPLLK"
     gene            6072..6989
                     /locus_tag="PLJ75_09125"
     CDS             6072..6989
                     /locus_tag="PLJ75_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPD05414.1"
                     /translation="MKRIGIIVNLNSRKYKLAKKPDDSYERLGGDHVIVRYTHSIDEI
                     ADVATEFKRAGMDYIAPCGGDGTLHHVITQFNKIYQNNLPPFLILKGGTMNNVATSIN
                     LTGDAESILKRAIATIKADNALPTIERKTMNVNNTYCFLFGNGLVADFLERYYTIGKS
                     YTKLVKLIYDSIVEALFNKNSNFFKGFDGSITCDQQTLPYANVLGLLAGTVETVGMGF
                     YPLYRANEKSDLFHVIVCAMAPKDLAKNIVKLKKGIPIRHEKFYENTVKTIDIHSDKP
                     FAYTMDGDLYYAENTLKVTTGINVQFVVV"
     gene            complement(7003..7881)
                     /gene="aroF"
                     /locus_tag="PLJ75_09130"
     CDS             complement(7003..7881)
                     /gene="aroF"
                     /locus_tag="PLJ75_09130"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPD05415.1"
                     /translation="MTISSTHHVELSNTITTNRDKSNPLFSKTYCDCTAIQLPYNTTV
                     SHHNVTIIAGPCSIESEEQIMATAHFVKKAGATILRGGAFKPRTSPYAFQGLGIEGLK
                     LLRKAGDYYTMPVVTEVLDSEDIAMVSQYADILQVGARNCQNFSLLKKLGKLQKPILL
                     KRGMMVTIKEYLMAAEYILAEGNTSVILCERGIRTFETETRNTLDISAVCVLKQKTHL
                     PVMVDPSHASGNWKLIHPLSLAAIAAGADGLMIECHINPEAALCDGDQSLRPDKFELL
                     MHDVKELCTMLNKAVV"
     gene            8317..8673
                     /locus_tag="PLJ75_09135"
     CDS             8317..8673
                     /locus_tag="PLJ75_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05416.1"
                     /translation="MKKKNKAALGVNGWYLSPSDNICLAKCIFDDFYREEIKEINKHE
                     IIAKIADCIGPEPSNLCIAEQIFEKIKFEIANFVIHHEYNCQRGVNADLLLWLDSKVN
                     PANLKKLNNFIDKYLK"
     gene            8670..9287
                     /locus_tag="PLJ75_09140"
     CDS             8670..9287
                     /locus_tag="PLJ75_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPD05417.1"
                     /translation="MKQRKKLLLHTCCGPCLTYVHSILSVEHEIAVFYYNPNIAPQAE
                     YAQRLSEVTQFAATHNIVLLTGRYNQADWFKAVRDQRFSGELSPRCYLCYSFRLKETF
                     DVAKQYKYDAVCTTLSVSPYKDAEKINEIGRSFAELYGIQFIEADFKQDGGYQHSIVL
                     SKQYGMYRQKYCGCVYSRLERNKNSLWSIKSNSYKKNMITANNKY"
     gene            9630..>12088
                     /locus_tag="PLJ75_09145"
     CDS             9630..>12088
                     /locus_tag="PLJ75_09145"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPD05418.1"
                     /translation="MAKKTFEAIRKNIESNPFYAIGLGLVIGIIIIGFLFTSVYKLFE
                     YQLFDVRFKIKPQLQQWEYLSFLDIDDNSISNVGQFPWPRYVYAEGIDVIKDLGIKQV
                     AFDIQFIDKSPRYAQPDIFKIVEDKIKAGKRITYEELNHAILDSDALFAGACAKNNVI
                     LSYSFLNRTLAEMNITPEERKKRDEAIARFEKIASIPVPEGSKNEYSRYIDPTRIIIQ
                     YPIPELIMNGKDFGYVDSDFDIDGIARRIRLIRVFNDRIYFHMALIMLMDILNVNKSD
                     VAIVHDRYLLLKNATDPLTLHKKDFKIPIDNRGMAYIHWSGDFKDTFNHVSFYALLEY
                     NQLKDTIHEYFDEQEIQSQNTERSKLYGELAQLDELLQKEKNVIAKQKVWLKINDLRR
                     RIYTIENGYIQNLERQLQEINQQLKTSDNVQLHEAKTNLNNFINASRLVLEVDRLKGH
                     SCIIGLTATASHDIGVTPLSSEYLMVGTYHNFINTVLQNAYIKEVPRWITYVLIIIFA
                     ILIALSVQRLSAIGTINVIVGSLIAYNIVNIALFAFSYIYIDQLGTSLALLLPSMVIS
                     SVKFMSEESQKRYIKNVFSKYMAPRVVDELIKNPNALQLGGELRNISIFFSDVEGFSS
                     ISEKLTPQQLVSLLNEYLSEMTDIILRYEGTVDKYEGDAIIAFFGAPLAFEDHPSRVC
                     LAAIDMKRRLAELREEWRKKGTFQLKVRMGINTGDAVVGNMGSRQRMDYTMMGDAVNL
                     ASRLEGANKYYNTYTMISETTYDSVKDVVDARELDLIRVIGKEQPIKVYELLGTKGNL
                     PDYMYDMLKKYNEGLQLFRDR"
BASE COUNT         4104 a         1889 c         2405 g         3690 t
ORIGIN      
        1 gattaattta atattaaaaa tagatgatta tgcaaaatat tgtatactta gaaacatagt
       61 tcatataatc gaaaattgaa gatactggca acaagaaaaa acaattttat acttataaca
      121 gtgaggtgct aatggaaact atacttaaaa aaattgaaaa acttatggtg caacatgatg
      181 cgataagccc ggagcttatt cagaaaaaaa atgtaaaatt gggattacgt aatccagatg
      241 gctcaggggt tgttgctggt attacatcaa aaggtacagt tattggatat gaaaaaatac
      301 cttcaggtga tgataaagtt actgttaaac cagttgaagg gaagttatat tattgcggct
      361 atgatgcctg tgaattagct gaaaaaattc aggaagaagg tagatatggt tttgatgaaa
      421 tcacctatct acttcttact ggcgagttac ctgataaaaa agatcttgag caatttagcg
      481 aggtgattgc cgataggcgt cctttaacaa aatttgaacg cagtatcata atggctgaag
      541 atgttaataa taatcagatg tacgcacttc attcagtgat ttcacattta aacagatgtg
      601 atcccgatcc agattcaact gatatcaagg atgttatcag acaatgtatc aacctgattg
      661 caaaagtacc tgttattgtg gcatataact ataatgtgat gaaatttaga aagggtggtg
      721 atttaattat ttgtaaacca aatgcagcgt ggagcacggc tgaaaacttt ttgtatttgt
      781 tgcacggcga tggggagcgc cctaatgaat atgaagcaaa attgtttgat ttagcattaa
      841 tgcttcatgc cgaacatggc ggaggtaata actcaacatt tacggtcaga acggtaacat
      901 caagcggtgc caatacatac atggcattgt gtgccggtat agcatctctc agtggacatt
      961 tgcatggagg agctaatgaa tcggtcatga aaatgatgaa ggatattaaa caaaatgtta
     1021 aagattggga tgatgatgat gagatagaac attatctgaa gcttatactg caaaaaaagg
     1081 cgtatgaccg cagcggtaaa atttatggca taggacatgc tgtttacaca aaatcagacc
     1141 cgcgtgccgt aatattcaga aaaaaggcag aggaatttgc aaaaatgaag aaaaatctgg
     1201 aagaatttac attatttaca aaggtcgctg atattggcac tcaacttttg ctgggtgata
     1261 aatcaaagat tgtatctcct aatgtagatt tttattcagg attaatatat aagatgatgg
     1321 gtatacctat ggaaatattt acaccaatat ttgcgatggc acgttttgct ggatggtctg
     1381 ctcataggat agaacaaata atacacaata agataatcag gcctgcgtat gcttcatcaa
     1441 tcgatgaata taaaaattac atcccattaa atgaacgata atatattggt ttatactata
     1501 ttataaaccc tgcgcgatgg tgcagggttt tttttagtat aaaatatagt tgacaaaatt
     1561 gtatcatttt tacaacataa cactgttatg ttaagaggag gttttatgaa atatgatgtc
     1621 atagtcattg gtgcaggtaa tgctggttta actgcagcag caacacttgc aaaaaaaggt
     1681 gtcaaaacac ttttaattga aaagcataac attcctggag ggtgtgctac cagtttttgc
     1741 aggggtcgtt ttgaatttga agtttcactt catcagttga gtggaatggg tacccctgaa
     1801 aggcctggtc cattacgaag cattcttgat agcgtgggtg tacttgatac attagagttt
     1861 gtccagatgg acaagcttta ccgggtggta gtaccgggtt tgattgatat tacactgcca
     1921 gcagataaag aagggattgt tactgcatta caggcaaaat ttcctgagga aaaagatgcc
     1981 attaaaaaat tttttgattt tgtatggcag tttttcatgg aggtaatagg agctttttat
     2041 ctaaatgatc cggatataaa tcaaaaaaaa tatccattat attataagta tgccttaaag
     2101 gatacacaat cagtattaaa tgaatatttt accaatccaa tgctaaaagc agtcataact
     2161 ccttactgga cctatatggg cttgcctcct tcaaaattat gttttagtga tatggcagcg
     2221 ttgttgtttt cttatgctga gtttaaacca tatcacataa aaggcggctc gcagatgctg
     2281 tcaaatagtt tgcttgagaa attttacagc aatggcggtg aagcattatt taatacagaa
     2341 gtacagaagc ttcttgttaa aaataatagc gtttatggag taaaaacatc agatggcact
     2401 gaatttcaat caaaatatgt tttatccaat gcatcagcaa tcagcacatt tgttgatatg
     2461 ctggatgacg acaaagtttc ccgtcgggtt attgaaccct ataaaggtag cagtattgga
     2521 acatcatttt ttactatata ttgcggtctt gattgtgaat acgataaggt tggcatcaat
     2581 gaagaaacca attttatatg caacactgcc aatccagaat atgattacga attatcaaaa
     2641 gttgtggata ccgaccagag ttcatttatt gtaacatgct acaatgtttt tgataatgca
     2701 atggttcctg aaggcaccag tcaggttgta ctggtaacca tgaaatatgc tgatgcatgg
     2761 cttaaagttc cacctgcaca gtattatgat gaaaaatgcc gctgtgcaga agcaatgctt
     2821 aacaatgctg aaagagtttt tcccgggctt gtaagccata ttgaagagat ggaaattgca
     2881 acgcctataa cccatatgcg ttatttaaat catcccggtg gaggagcata cggatttgat
     2941 cagtatgtaa aagattctac tttgtttatt tcgcctaaac cgcccatcaa tggtctttat
     3001 tgtgcaggga gctggtacgg ttctgcaggc tatcagccaa cccttacatc aggacattca
     3061 gcagcaatgg caatagtaaa agatatacgt aacaaatagg aggggatgat gaatagtgaa
     3121 atactgaaac agttagatgg ttatgataac gttataaacg aacgagaggt gctcaaacgg
     3181 tatggttatg attatagatt ggatcgaggg tttgtaagaa aatatcttga gcggttgcat
     3241 ccacggtata ttactaccaa aattattgac atcatagaag aaacgccgtc aaccaaaaca
     3301 ttgcgtttgg attcagtgga aaaatattta ccgccatttc aggcaggaca gtacatatct
     3361 gttaatgttg atattgatgg aatcagaact tcacggccat atagcatttc ctcatcacct
     3421 gatgagacag catattatga tatcactatc agacgagttg aaggtgggtt tgtatctcat
     3481 tacttgcttg attcaacaaa gattggcaac acattgcaaa tatcatcacc cgcaggtaat
     3541 tttatccata atcctattat tcacagcaat gaacttgttt ttattgcagg cggtagcggc
     3601 ataacaccat ttaaatccat gattcaacat gctgttaaca ccggtatcag cagggatata
     3661 tggcttatct atggaaacaa aaattcagaa gaattattgt ttcatgatta ctttaccatc
     3721 gttgcaaaaa aataccccac tatacgctat gtgcaggtat tggaggaggg cggggccagc
     3781 tataatgggt atataacggc aaaaataatt aaggaagttg tgggcgatag ttccagtaaa
     3841 actttttttg tatgtggacc tcagggttta tataaacata tcctcaacga attacaaacc
     3901 ttacaattaa agcccagaca gatacgcatg gagatgtatg gagtgcccac agatgttttt
     3961 aaacaaagtg gatggcctga agatgttacg ccagattcga aatttaaaat tactattaaa
     4021 aataaaaaag aatttacggt agcagcaggt gaaccattgt taaactcaat ggagaaaaat
     4081 ggagtcattg taccatcgtt atgcagaagc ggggaatgca gcttatgcag ggttaaactt
     4141 atatcaggga aagtgtatca gccagaaggt gttaagctgc gtcaggctga ccgacgtttt
     4201 ggttatatcc attcatgtgc tgctttccct atcacggatt gtgaaataat gctataaaaa
     4261 ttaattgatt gaaggagatg ttatgatcac tactaaaatt acagagatgt ttggtgttaa
     4321 gtatccaatt atttgtggag ctatgatgtg gttatgtaaa ccaaaacttt gtgcagcaat
     4381 ttcaaacgct ggtggtattg gaaatttaac ggcaggaaat tatagctcag aagaggagtt
     4441 tagaaatgct atccatgaaa cgcggaaact aactgataaa cctttttttg ttaatgtaac
     4501 aattttgcca tctgttcgca ttactggtga gcatcataag atgtatttaa aggtatgtgc
     4561 tgaagaaaag gtagctggca tagaaatatc aggatcacct attgataaag catgcggcat
     4621 ggaatatatt gagatgctta aaaaagcagg ggttaaactt tttcacaaag taggtgcggt
     4681 acgccatgct ctccatgcag aacaggttgg ttatgatgga atttatgccg caggaattga
     4741 agaaggaggg catcctttaa gtgatgatgt ttcaacgatg gtattaacaa caaagattgc
     4801 taaaacagta aaattaccgg ttattacggt aggcggcatt gctgatggta aaacaatggc
     4861 agctgctttc atgttaggag ctgagggagt tatgatggca agcaggttta ttgcaaccaa
     4921 ggaatgtgaa gtccatgaca atataaagca ggagcttgta aaaaggcagg aaaatgaaac
     4981 aaccttaatt atgaagagct tacatcttca gggacgtgct ttaaagaatg atgtggtcaa
     5041 taaaatactg gatattgaac agcgcggtgg cggctttgaa gagcttttcc cgcttatttc
     5101 aggcgaacgg atgagcaagg catggaacaa tggcgatgta aatgctgcac cattaatggt
     5161 agggcaatcc atagggctca ttgatgatat accatcatgt gctgaattgc ttcaaaggat
     5221 ggagaaagaa gctgaaacgc aaattaaaaa gatttatagt aacatactgt aataaatagc
     5281 gcgcaggcaa tctgcgaaat tgcctgcgca tacatttctt cattattgca agtgagaaga
     5341 atatttattt gaggagtggt atcaatctct tttttatggc ttctgctatt aagagatgtc
     5401 ctttttcatt agggtagcga taatcatggg taagcagcga ttccagagta tcattattct
     5461 catacagata attcatccat tcaatgtgca cagtagctaa atgacaatgt aaatccaatg
     5521 ctacttcacg caccgttcgg gtaaagacgt tgacagcgtc catatcatac tgatttggga
     5581 caagatgcag cgttggcata ataatatgct tgatgtatga ttcacgcgcc ttttgtacca
     5641 tcctgactaa attctcttta aattcagacc tatataccgg gcggtacatg tcgtccatgc
     5701 caaaatgaat aatcagcaca tctggtttat gctgcaatac cctgtcaaaa tgccagatcc
     5761 cttcaaagga gctatcgcca aaatgagata cattaatgat gctatgctgt ttgattgcct
     5821 catcgcttga aagcattgca acgtatgatt gagtggttgc ataccctgaa gttatagagc
     5881 cgcctgatag tactattgtt gccatatttt tttcatagca atttttttaa gaaaaataaa
     5941 tattcacaat aataagtcaa atcattttat ataggtaaaa tcaaattgac agatactcaa
     6001 caacatttgt atggtaaata ccagaatgat agtatacatt tttttataaa aaagcaaaag
     6061 gtataaactc aatgaaaagg attggaatta ttgttaatct taattcacga aaatataaat
     6121 tagcaaaaaa acctgatgat agctatgaac ggctgggtgg cgaccatgtg attgtgcgct
     6181 atacacattc aatagatgaa attgcagatg tggcaacaga gtttaaaagg gcaggaatgg
     6241 attacatagc accctgtggt ggcgatggga cattacatca tgttattact cagtttaata
     6301 aaatttatca aaacaatcta ccgccatttt tgattttgaa aggcggtact atgaataacg
     6361 ttgctaccag tattaactta acaggtgatg cagagtcaat tttaaagcgt gccattgcaa
     6421 caataaaagc tgataatgca ttgccaacca tagaacgaaa aacaatgaat gtaaataata
     6481 cctattgttt tttatttggg aatgggcttg tagctgattt tttagaaaga tattatacta
     6541 taggcaaaag ctatactaaa cttgtaaagc ttatttatga cagtattgtg gaagctcttt
     6601 tcaataaaaa ctcaaatttt tttaagggtt ttgatggtag cataacctgt gaccagcaaa
     6661 cattaccgta tgccaatgtt cttggattgc tggccggtac agttgaaaca gttggtatgg
     6721 gtttttatcc tctctataga gcaaatgaga aaagtgattt gtttcatgtc attgtatgtg
     6781 caatggctcc caaagatctg gctaaaaata ttgttaaatt aaaaaagggt attcccatac
     6841 gtcatgaaaa attttatgaa aatacagtta aaacaattga tatacattca gataaacctt
     6901 ttgcctatac tatggatggc gatttatact atgctgaaaa tacattaaaa gtaacaacag
     6961 ggattaatgt acagtttgtt gttgtataag atattacaaa tatcatacga cagctttatt
     7021 cagcattgtg cataattctt ttacatcatg catcagcaat tcaaatttat ccggtcgcaa
     7081 tgattgatca ccatcacaaa gagcagcttc cggattgata tgacattcta tcatgagtcc
     7141 atctgcacct gcagcaattg cagctaatga taatggatgg atgagtttcc aattgccgga
     7201 ggcatgagaa gggtctacca ttacaggaag atgagttttt tgctttaata cacaaacagc
     7261 agatatatcc agtgtattcc gtgtttctgt ttcaaatgtt ctgataccgc gttcacatag
     7321 tataacactg gtattaccct ctgctaatat atattctgca gccattaaat attctttaat
     7381 ggtaaccatc ataccgcgtt taagcaatat aggcttttgt agtttaccta atttttttaa
     7441 taaggaaaaa ttttgacaat tccgtgcacc aacctgaaga atatctgcat attggctgac
     7501 catagcaata tcttctgaat ccagaacttc ggtaacaact ggcatggtgt agtaatcacc
     7561 ggcttttcgt aacaatttta atccctcaat tcccagcccc tgaaaagcat agggggatgt
     7621 tcgtggttta aaagcaccac cacgcaaaat agttgcacca gcctttttca caaaatgggc
     7681 agtagccata atctgttctt cagattcaat agagcaggga cctgcaataa tggttacgtt
     7741 gtgatggcta acagttgtgt tatatggcaa ttgtatagca gtacaatcgc aatatgtttt
     7801 actgaataat ggattggact tgtccctgtt tgttgtaatt gtgtttgata gctctacatg
     7861 atgagtgctg gatatggtca tatactcctc cgtttaatag tattaatgtt taatgagcct
     7921 ggaggatggc gaccttatat atttgtacta caataaaaaa ggccgccagc tacccgcctg
     7981 cggccccttg tcaatataac agcacaaaca aaagctacag acgagctttg ttataaaaat
     8041 aataaaacca aaaatagctt ttgcctgtaa tgttatatac aattatattc ataatatgta
     8101 atgtattaaa attagtaaat ttatttacat aaaaattgct acatttaaaa attgtcaaga
     8161 gaaaaaataa aaatttttta attatccata aaatcattga tcaaaattat tgacatttca
     8221 taaataattt tttatattta tctatattta ttttaaattt ttttagcgat agtgtttaat
     8281 aattcatact gccctatgaa aaaaagagga aaaacaatga aaaaaaagaa caaggctgct
     8341 ttaggggtaa atggatggta tttatcccct tctgataata tttgtctggc taaatgtatt
     8401 tttgatgatt tttacagaga agagattaaa gagataaata aacatgaaat tattgcaaag
     8461 attgccgatt gtataggtcc tgaaccgtca aacttatgta tagctgagca gatttttgaa
     8521 aagataaaat ttgaaattgc caacttcgtc attcaccatg aatataactg ccagcgcggc
     8581 gtaaatgctg atttattgtt gtggcttgat agcaaggtta accctgcaaa cctgaaaaag
     8641 ttgaataatt ttattgataa gtatcttaag tgaaacaaag gaaaaaactg cttctccaca
     8701 cgtgttgtgg cccatgttta acctatgtcc attcaattct atctgttgaa catgagatag
     8761 cggtttttta ctataatcca aatattgctc cacaagcaga atatgcacaa cgattatcag
     8821 aagtaacgca atttgcagca acacataaca ttgtactgct aacgggcaga tacaatcaag
     8881 cagattggtt taaagcagtc agggatcaaa gattttcagg ggaactatcg ccacgctgct
     8941 atttgtgcta ttctttcaga ttaaaagaaa catttgatgt agcaaaacaa tacaagtatg
     9001 atgcagtttg tactacgcta tcagtaagtc catataaaga tgctgaaaaa atcaatgaga
     9061 ttgggcggtc atttgcagaa ttgtatggaa tacaatttat tgaagcggac tttaagcaag
     9121 acggcgggta tcagcattca attgtattgt caaaacaata tggcatgtat cggcagaaat
     9181 attgtggctg tgtgtattcc aggcttgaga gaaataaaaa ttcattgtgg tcaattaaaa
     9241 gtaatagtta taaaaaaaat atgataactg caaacaataa gtattgatac aggttccttg
     9301 ttcaaaggtt actatcgtac aattactatt gtactataag cttttttccc tactaacagg
     9361 taattgcaag gctaaatagt ttatcaccac tatgtgccct ccatgtaata tacatgatat
     9421 tgtacattgg taaaaaatac tgcaatataa acactactta ttgcaggata tgaagatggg
     9481 caatgtgata ttaccgtatt atatccaaaa aagataagac gatagaaaaa aaattaaaaa
     9541 atagtgtttg gtattctgct tgtaaattta gaatgtatat tataatgtat tacctattga
     9601 tttgtacatt tatataaatc gggtgatata tggcaaaaaa aacttttgaa gcaatcagaa
     9661 aaaatattga atcaaatccg ttctatgcaa ttggtttagg attggtaata ggaatcatta
     9721 ttattggatt tttatttacc agcgtatata agctatttga atatcaactg tttgatgtaa
     9781 ggtttaagat taaacctcaa ttacagcaat gggaatatct ttcattcctt gatattgatg
     9841 acaacagtat aagtaatgtt ggacaattcc catggccacg gtatgtgtat gccgaaggga
     9901 ttgatgtcat taaagacctt ggtattaaac aggtggcatt tgatattcaa ttcatagata
     9961 aatcaccgcg ttatgcacaa cctgatatct tcaagattgt tgaagacaaa ataaaagctg
    10021 gcaaacgaat aacctatgag gaattaaacc atgcaatact ggatagtgat gcattatttg
    10081 ccggggcatg tgcaaaaaat aatgtcattt tatcatattc gtttttgaat agaacgcttg
    10141 ctgaaatgaa tataacccct gaagaaagaa agaagcgtga tgaagccatt gcacgctttg
    10201 aaaaaattgc atctatacct gttcctgaag gaagtaaaaa tgaatattca cgatatattg
    10261 atcctactcg tattattatt caatatccaa tcccggaatt aataatgaat gggaaagatt
    10321 ttggctatgt cgacagcgat tttgatattg atggcatcgc tcgcaggata cgcctcatac
    10381 gagtattcaa tgacagaata tattttcata tggcactgat tatgctcatg gatatactta
    10441 atgttaataa aagcgatgtg gcaatagttc atgaccggta tctgctatta aagaatgcta
    10501 ctgatccttt gacactgcat aaaaaggatt ttaaaatacc aatcgataat agaggaatgg
    10561 catatataca ttggagtggt gattttaagg atacctttaa ccatgtatca ttttatgcct
    10621 tattggaata taatcagctt aaagatacca tccatgaata ttttgatgaa caggagatac
    10681 aatcacaaaa tacagaacgg agcaagttgt atggtgaact ggcacagtta gatgagttat
    10741 tgcaaaaaga aaaaaatgtc atagcaaagc aaaaagtatg gttaaaaata aatgatttga
    10801 gacggcgtat ctataccata gaaaatggct atattcaaaa tcttgaacgg caattacagg
    10861 aaattaatca acaacttaaa acttctgata atgtccaatt gcatgaggca aaaacaaatc
    10921 ttaataattt tatcaatgca tcaaggcttg ttcttgaagt ggacagatta aaaggtcatt
    10981 cgtgtataat aggtttaacc gctacagcct cgcatgatat tggggtaacc ccgttatctt
    11041 ctgaatatct tatggtaggc acctatcata attttattaa tactgtatta caaaatgcat
    11101 atatcaaaga ggtgcctcgt tggattactt atgtgctcat tataattttt gcaatactta
    11161 ttgctttatc ggtgcagcga ctaagtgcta taggaaccat aaatgtaata gttggttcac
    11221 tgattgccta taacattgtc aatattgcat tatttgcatt tagctatata tacattgatc
    11281 aattgggtac ttcgttagcg ttattattac cttcaatggt tataagcagt gtaaaattta
    11341 tgagtgaaga aagtcaaaaa cgatacatta aaaatgtttt ttcaaaatat atggcgcctc
    11401 gtgtggttga tgaactgatt aaaaatccca atgcattgca attagggggt gaactacgaa
    11461 atataagcat atttttttct gatgtggagg gattttcatc catttcggaa aagttaacac
    11521 ctcaacaact tgtttcctta cttaacgaat atttatctga aatgaccgac ataatattgc
    11581 ggtatgaagg tacagtggat aaatatgaag gcgatgcaat tattgcgttc tttggtgctc
    11641 cattagcttt tgaagaccat ccttcacggg tgtgtttggc agccattgat atgaaaagac
    11701 gtctggcaga gttgagggaa gaatggcgca aaaaaggtac attccagctt aaagtacgga
    11761 tgggtatcaa taccggggat gctgtagttg gcaacatggg ttcgcgccag cgaatggatt
    11821 atacaatgat gggcgatgct gtaaatttag catcccggct tgaaggtgca aataaatatt
    11881 ataataccta cacaatgata agcgaaacta cgtatgacag cgtaaaagat gtagttgatg
    11941 cacgtgagct ggacttaata cgggtgattg gcaaagagca gccaattaaa gtgtatgaat
    12001 tattagggac aaaaggaaat cttcctgact atatgtatga tatgttgaaa aaatataatg
    12061 aagggttgca attatttcga gacagaca
//
LOCUS       DAOOJH010000070        12045 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4869_length_12045_cov_10.358215, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000070 DAOOJH010000000
VERSION     DAOOJH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12045
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4869_length_12045_cov_10.358215"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLJ75_09150"
     CDS             complement(<1..980)
                     /locus_tag="PLJ75_09150"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPD05419.1"
                     /translation="MKPKDGYSAEELLNSTQGLSYNDFIILPGYIDFNPDDVDLETNF
                     TRNVTLKRPLVSSPMDTVTESKMAISLALLGGIGIIHYNNTIEEQAAHVRKVKRYENG
                     FITDPVVLSPEHTIAHIDEIRESHGFSGIPITVDGKLNSKLVGIVTNRDIDFETDRTK
                     KLKEVMTTNLVTAGVGITLAQANEILKQSKKGKLPIVDNEGRLVALMSRNDLVTNKEF
                     PFASKDEKKQLMVGAAISTKDESKERLQELIKAGVNVVIIDAAQGNSIYQVEMIKYIK
                     KKYPDLDVVAGNVVTVEQCENLIKAGADALRIGMGPGSICTTQVTMAVGRA"
     gene            complement(1243..2592)
                     /gene="radA"
                     /locus_tag="PLJ75_09155"
     CDS             complement(1243..2592)
                     /gene="radA"
                     /locus_tag="PLJ75_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPD05420.1"
                     /translation="MAKKLKSIFVCNECGATFQKWLGRCPECLQWNTIVEELSETITQ
                     KNEIPFENSIHPLSEMHSRQIDRIATGIYEFDLVCGGGIVPGSILLLGGEPGIGKSTL
                     ALQIAQQLTTLYCSGEETMQQIYLRSCRLQIKPEKIHISSVTNIDNIELLIKTNQPQL
                     VIIDSIQTLYSSEISSPVGSVSQIRYTASKLTEIAKQTGTCIVLIGHITKDGSIAGPK
                     VLEHIVDTVLYFEGDFTRDYRILRSFKNRFGSINEIALFTMTSQGLIEVKDKNKLFVQ
                     SQKASFPGSVISATIEGTRTILFEVQSLVTATSFTNPRRMADGFDVNRLNLLVAVIEK
                     HVKLSLSSFDIFINVAGGFAVNDTSCDLAIAAAIISSLKNFAISHSTGIIGELSLSGQ
                     IRPVSHILRRITEFTNSGVTSIILSKNNINEVSLPDVATTLIPITSIVELTKLLQPQ"
     gene            complement(2595..3470)
                     /locus_tag="PLJ75_09160"
     CDS             complement(2595..3470)
                     /locus_tag="PLJ75_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPD05421.1"
                     /translation="MRMSNEARVGFMVMISFTLFVVLVGILTKFNISQKGYRIHVYFG
                     FLNDLRVGAPVKIAGGIKIGYVDEIRQTGEKTDVVVWIDKGYRLSKAATFSIFTSGVI
                     GEKYINVVIPPLQEDIGFLKEGDIKYGIDPASFDQMLQTFQSFMQDKSGAQILAEIFQ
                     NSNKFVANLNKMVDENRYDVRQSVGTARTAITTFSQQLQQFMQQMNVISKNMAYISEK
                     NREDITITLQNLSELTTSINKIAYRLEKGRGTMGKFLYDEEIYTNIRDASISAKELFS
                     RLEKDPSLLLFKQKK"
     gene            3769..5022
                     /gene="purD"
                     /locus_tag="PLJ75_09165"
     CDS             3769..5022
                     /gene="purD"
                     /locus_tag="PLJ75_09165"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPD05422.1"
                     /translation="MRYLVIGSGGREHAIAWRLLHDGSATEVYVAPGNGGIDDRYRVA
                     IAINDFESMYTFCKSKSIDVVVVGPEAPLVNGIVDFLHNKGIRVFGPTAKAAQLEGSK
                     LFAKYIMQKYNVPTAQFREFKDKNDLLHYIESLTEYPIVIKLDGLAAGKGVGVCNTKH
                     DAIQFINTMVNHDTRVFVEDFCAGEEASVLGISDGKTVVPFIAAQDHKRAFDNDEGPN
                     TGGMGAYAPAPIVDSRILGRVHREILQPVIQGMNKEGIPFAGILYAGIIIKDGMIKVL
                     EFNVRFGDPETQVLLPLLNGKLGDYINAAIDGTLSSTNLSFRNKHAVTVVLASGGYPG
                     SYSTGQPIHGLDQIHNDILIFHAGTKREGNTIVANGGRVLNVTAIADSLEEAYNKVYS
                     VIPLLSFEGMHYRKDIGFRALKKRA"
     gene            complement(4957..5487)
                     /locus_tag="PLJ75_09170"
     CDS             complement(4957..5487)
                     /locus_tag="PLJ75_09170"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPD05423.1"
                     /translation="MKSLYYYNELVKRIDAFTSDIIAVYGTLLQCKEGCSGCCILESV
                     FPVEMVAILHWYSNQSPSVREAITNTKDNEYCIFLHEHTCRIYPYRPIICRTHGCPIM
                     VDTTIDVCPCNNGISFAANHILNVEHCNSMLAGINLLFIKEVNIPLLQKERIDLRTFF
                     QSSESDILSIVHSLKR"
     gene            complement(5492..7228)
                     /locus_tag="PLJ75_09175"
     CDS             complement(5492..7228)
                     /locus_tag="PLJ75_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPD05424.1"
                     /translation="MTIQPQSDILYVKPYTDAYAIINIRLGGRGYIYANPKGEGTGKP
                     LVAKATTHDPIEFKNTRYLPGTMHISPIDNKPVYIYKNQTSLCIPFTVKTNREGLYSF
                     TISIDALLCNDSSCTPITGTIAQKVKVKNDAADFPQSHIAPYKEPQQMTYLEQSHNNY
                     TFNAIDMGYSSKGILHAIIFGIIAGFILNFMPCVLPVISLKLFSFVSYAHNNPRQIKS
                     MGLLFTLGIITSFIILAALAAFAGYNWGELFQHKTFIIVMAAIIFSMALSFFDIYTFN
                     VPSLPSKLQSANPYIDSYFKGVLATILATPCSGPFLGAILTWTLTQHPIVIFMIFINI
                     GIGMSLPYLILAMYPSLVRFIPKPGEWMIIFEQCMGFLLVATSIYLITLLDITALTQA
                     LWFILLLSLGLWQWGIFGNIARPLIHRITSFIILLAIVIISGLMLFTTKPSVSRIPLK
                     QLDWDAIVHNNSSHVSMIIFTADWCPNCKLVEGTTLKSKKVIDFIKSNDIHTYKADIT
                     TKNELAEELMQSLGARSIPFVAIFPKGEYFYQPLILRDMYTSNDLLKALQKAMDISNS
                     QQTLQLMTIPHH"
     gene            7500..7670
                     /locus_tag="PLJ75_09180"
     CDS             7500..7670
                     /locus_tag="PLJ75_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05425.1"
                     /translation="MMAQQITVVEQSIEQLNNLLKGLAAVRTGLSEKLVEVNVKTQGR
                     IEGLGTIIDSYQ"
     gene            complement(7767..7916)
                     /locus_tag="PLJ75_09185"
     CDS             complement(7767..7916)
                     /locus_tag="PLJ75_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05426.1"
                     /translation="MEGKITEHETAFLHQGIIGKQIKTHGGILSSGMVLFVRSSISSI
                     ILLEH"
     gene            8278..8910
                     /locus_tag="PLJ75_09190"
     CDS             8278..8910
                     /locus_tag="PLJ75_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05427.1"
                     /translation="MEKKLLIIAAGLLLLCQTIFAANKEINGTLGTSYADNPSKYGLD
                     ISLHYNWLLDPYFLTGLESGFFWVQWDRKIGVKQEGSVYVDVKADSNAYTIPIMFNAQ
                     VRLPNLQSKLYVVPFATVGFGYSFMILHYSRPDFTDSRTGKTYDSDSITKLFKGLSWE
                     LLFGVSLKPATDSKVDFYAECGYRKLPLKSGDLELDMSGFLLRFGVRYPL"
     gene            9008..9952
                     /locus_tag="PLJ75_09195"
     CDS             9008..9952
                     /locus_tag="PLJ75_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPD05428.1"
                     /translation="MVPTAYLFDPVYMDHDTGWGHPERPERLVAIDQKLRQQPYYKSL
                     IKVEPKIGAKRYIELVHSPDYVKRVKEEIESGVHYLDSMDTAVCKRSYEVALLAVGGS
                     LNMCDAIISGEAIRGFCAARPPGHHAEYDYAAGFCIFNNIAIAAKYLQVEHGINKIAI
                     VDWDVHHGNGTQHTFERDRTVLYISTHQYPHYPGTGARSERGKEEGEGYTLNFPMPAG
                     SGDTEYMDVFENHIVPALMKFEPEIVLLSAGFDAHNADPLSYIGLSTEAYYKFTVLIK
                     QAANEYSGGRMIAFLEGGYDLEALANSVDMMMRAFIED"
     gene            10376..>12045
                     /locus_tag="PLJ75_09200"
     CDS             10376..>12045
                     /locus_tag="PLJ75_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05429.1"
                     /translation="MYPLLFTPITINTLQIKNRIAYPALGLLYSYDSLLNDRYYEYFR
                     EKAKGGAGLVTVGPVGIDIAGSGRMALSLADDDKIPSFKKLTTIIKSEGAQTFVQLFH
                     AGAYSYSKLTDDVQPIAPSSVYSKYSKQVPREMTLEDIEMVQTAFVKAALRAQEAGFD
                     GIEIIASAGYLITQFLSPITNKRTDRYGGSFENRVRFPREVIEKVRKAVGFDFPLGIR
                     MAGNDFVKGSNTDRETPLFAKVYEDAGVDIINVTGGWHEAKIPQLPMELPRMGYAYLA
                     ATIKKAVHVPVMASNRIATPEAAEKILRDSMADMVNLGRVLIADPDWPTKAKEGKTNL
                     IRPCVGCNQGCTDTLFSGMPVGCVLNARAGYEGERNIMKTDTPLRIMVIGAGPGGLEA
                     AVRAKEAGHRVELYEMSTDIGGQLWIAAAPPHKHELLEIIRYYRAMVKHLDIPVFYNT
                     AMTVELIKEKNPDHVIIATGAEAVMPPIEGIGLPHVYSAWDVLDNDPVLGGNVAVIGG
                     GAVGIETALFIANKGTISPEILHFLMFFDAEPVDRLKELITQGTSKVT"
BASE COUNT         3775 a         1976 c         2453 g         3841 t
ORIGIN      
        1 gcgcgtccta ccgccatggt aacctgtgtt gtgcatattg atccgggacc catacctatt
       61 ctgagtgcat cagcaccggc tttaattaga ttttcacact gttcaacagt tactacattg
      121 cctgcaacta cgtcaagatc agggtatttc ttctttatat atttaatcat ttcaacctga
      181 tatattgaat tgccctgagc agcatcaatg atgacaacat taacaccggc ttttataagt
      241 tcttgaagac gttctttgga ttcatccttt gttgaaatag cagcaccaac cattaattgc
      301 tttttttcat ccttagaggc aaaagggaat tctttattgg taacaaggtc atttctgctc
      361 atcaaggcta caagtcttcc ctcattatcc actattggca gttttccttt ttttgattgt
      421 ttcagtattt catttgcctg tgccagagtt atgcctacac ctgcagtgac aagattggta
      481 gtcattacct ctttcaattt ttttgttctg tctgtttcaa agtcgatatc acgattggta
      541 acaatgccta caagctttga gttaagttta ccatcaacag ttatgggtat acctgaaaat
      601 ccatggcttt ctctgatttc atcgatgtgt gctatggtat gttcaggtga aagcaccact
      661 ggatcagtga taaaaccgtt ttcatatcgt tttacttttc taacatgggc agcctgctct
      721 tcaattgtat tattgtaatg aattattccg atccctccta acaaagccag gctaattgcc
      781 attttagatt cggtcaccgt gtccatagga cttgatacta aagggcgttt taatgttaca
      841 ttgcgtgtaa aatttgtttc cagatcaaca tcatcaggat taaaatcaat ataacctgga
      901 agtatgataa aatcattata tgaaagtcct tgagtagaat ttagaagttc ttcggctgaa
      961 tagccatctt ttggtttcat acagtacctc aaaaagtata aagcgttata tttttaatag
     1021 tagagttcaa tttacaagac atatcgtaat tatatatgat atagcattgt ttaggattta
     1081 tttcaatatt tgctatataa ttatttcaat ataatgaaca atcaaaacta tgagaacatc
     1141 tgcaccataa tttttattta gatcaaaatc tgaataaggt attttgtatc actgctccct
     1201 gcctataaaa attttgaaaa ataaagttgt atttccacat aattattgag gctgcaatag
     1261 tttggtcaac tcaacaatgg aagtaatggg gatcaatgtt gttgcaacat ccggtagaga
     1321 cacttcgttg atattattct ttgataaaat aatggaagta accccggaat tagtaaattc
     1381 tgttattcta cggagtatgt ggctaacggg gcgtatctgc cctgatagtg acagctcgcc
     1441 aataataccg gttgaatggg atatagcaaa attttttaag cttgatataa ttgctgctgc
     1501 aatggctaaa tcacatgagg tatcgttaac tgcaaagcct ccagcaacat ttataaatat
     1561 atcaaatgag ctgagcgaca gcttgacatg tttctcaata actgccacca acagattaag
     1621 cctgttaaca tcaaatccat ctgccatcct tcggggattg gtaaagcttg ttgcagtaac
     1681 cagtgattgt acttcaaaaa gaatggttcg ggttccttca atagtggcgc ttatgacgct
     1741 tccagggaaa cttgcctttt gcgattgtac aaataatttg tttttatctt ttacctctat
     1801 taaaccttga gaggtcatgg taaaaagagc tatctcattg attgaaccaa aacggttttt
     1861 gaaagagcgc aatatgcgat agtcacgggt aaaatctcct tcaaaatata aaacagtatc
     1921 cacaatatgc tccagcacct ttggaccggc aatggaacca tccttggtaa tatgcccaat
     1981 gagcacaata caggttcccg tttgtttggc aatttcagta agttttgatg ctgtgtagcg
     2041 tatctggctt actgaaccaa caggtgaact aatctctgaa gagtaaagtg tttgtatgga
     2101 gtcgataata acaagctgag gctggtttgt ttttattaat aattcaatat tatcgatatt
     2161 ggtaactgat gagatgtgaa ttttttcagg cttaatctgt aaccggcatg aacgcaggta
     2221 tatctgctgc atggtttctt caccggaaca atacagggtg gtaagctgtt gtgctatctg
     2281 taacgctaaa gtagatttcc caatacccgg ttcgcctcct aaaagcagta ttgaaccggg
     2341 gacaatccca ccaccgcata ctaaatcaaa ttcatatatt cctgttgcaa tgcgatctat
     2401 ttgcctggaa tgcatctctg aaagcggatg tatggaattt tcaaaaggga tttcattttt
     2461 ttgtgtaatt gtctccgata gttcctcaac aatggtattc cactgaagac attcagggca
     2521 acgtcccaac cacttttgaa aggttgcacc gcactcattg caaacaaaaa ttgatttaag
     2581 tttttttgcc atgattattt tttttgttta aaaagcagta atgacggatc tttctcaagt
     2641 cttgagaata actcttttgc tgagattgag gcatcacgga tgtttgtata tatctcctca
     2701 tcataaagaa attttcccat tgtacctcta cctttttcaa ggcgatatgc aatcttattt
     2761 atacttgttg tcagttctga taaattttgc agggtaatgg ttatatcttc acgatttttt
     2821 tcggatatat acgccatatt tttggaaatg acattcatct gctgcataaa ttgttgtagc
     2881 tgttgtgaaa atgttgtaat agctgtacgt gcagtaccaa ccgattggcg cacatcataa
     2941 cggttttcat caaccatttt attaagatta gcaacaaatt tatttgaatt ttggaaaatt
     3001 tcggcaagta tctgtgcacc gcttttatcc tgcataaaac tttgaaaagt ttgcagcatc
     3061 tggtcaaagc ttgccgggtc aataccatat tttatgtcgc cttctttcag gaaacctata
     3121 tcttcttgca atggaggtat aacaacattg atatattttt cgccaatcac ccctgaagta
     3181 aaaatggaaa aggtagctgc ttttgataat cggtaacctt tatcaatcca cactacgaca
     3241 tcagtctttt caccggtttg tcgtatttca tctacatatc ctatctttat accacctgca
     3301 attttaaccg gcgcaccaac tctcagatca tttaaaaacc caaaatacac atgtatacga
     3361 tacccttttt gagatatatt aaatttggtt aaaatcccta ccagaacaac aaaaagggtg
     3421 aagcttatca taaccataaa cccaactctt gcttcattac tcatccgcat ataaacctcc
     3481 caatgaagga tgattgtatc caatatactt aatttcaata ttattcaacc attattcata
     3541 ttgagaaata aaaatattcg tagattcaac aattaagtgc aacccttttc tgtaaaaaat
     3601 aatgtataaa atgattgaaa aaaacagcag tatatttata agcaaattgt cagttgtata
     3661 atttgccaaa caataatgca gattgtattt cctatggtag ccatacaata tatggtatac
     3721 ttacccgagc aatggcaatg ttcatctatt tttataaaga ggtagccaat gagatatctg
     3781 gtgattggtt ctggtgggcg cgaacatgca atagcatgga gattattaca tgatggaagt
     3841 gctactgaag tgtatgtagc acctggtaat ggcgggattg atgataggta cagggttgcc
     3901 atagcaatca atgattttga gtcaatgtat actttttgta aaagcaaaag tattgatgtc
     3961 gttgttgttg gtcctgaagc cccgcttgtc aatggtattg ttgattttct tcacaataaa
     4021 ggtattcggg tttttggtcc cacagcaaag gctgcacaac tagaaggaag caagctattt
     4081 gcaaagtaca tcatgcaaaa atataatgtt cctactgctc aattcagaga atttaaagat
     4141 aaaaacgatt tattgcacta tattgaatcg cttactgaat atccaatagt tataaagctg
     4201 gatggtcttg ctgccggtaa gggtgttggt gtgtgcaata caaaacatga tgctatacag
     4261 tttattaata ctatggtcaa ccatgacaca agggtgtttg ttgaggattt ttgcgctggt
     4321 gaggaagcct ctgtattggg aataagtgac gggaagacag tagtaccgtt tatagcagcg
     4381 caggatcata agcgtgcatt tgataatgat gaaggtccca atactggagg aatgggcgcc
     4441 tatgcaccag caccaatagt tgatagcaga attttaggaa gggttcacag agagatttta
     4501 cagccggtta ttcaggggat gaataaagaa ggcatccctt ttgcagggat tttgtatgcg
     4561 ggcattatca tcaaggatgg gatgattaag gtgttagaat ttaatgtacg atttggtgat
     4621 cccgaaacac aggtattgct gcctctttta aatggcaaat tgggtgatta tatcaatgct
     4681 gcaatagatg gcacattatc atcaacaaat ctttcattcc ggaataaaca cgctgttacg
     4741 gttgttttag catcaggtgg ttatcctggt tcatactcaa cagggcaacc aatacatgga
     4801 ttggaccaaa tccataatga tatccttata tttcatgcag gtacaaagcg tgaaggaaat
     4861 actattgttg ccaatggggg aagggtgctc aatgtaaccg ccattgctga cagccttgag
     4921 gaggcatata ataaagtata tagtgtaata ccattgttat cgtttgaggg aatgcactat
     4981 agaaaggata tcggattcag agctttgaaa aaacgtgcgt agatcaatcc gctctttttg
     5041 taataacggt atattcactt cttttataaa taaaaggtta atgcctgcaa gcatgctgtt
     5101 gcaatgttct acatttaaga tgtgattggc tgcaaacgaa ataccattat tgcatgggca
     5161 aacatcgatt gttgtatcca ccataattgg acatccatga gttctgcaaa taataggtcg
     5221 gtaagggtat attctgcatg tatgctcatg taagaatata caatattcat tatctttggt
     5281 gtttgttatg gcttcccgta cggagggtga ttggttacta taccaatgaa ggatggcaac
     5341 catctcaacg gggaaaacac tttccagtat gcaacatccg ctgcagcctt ctttacattg
     5401 taacaaggtg ccatatacag caataatgtc actggtaaat gcatctatcc tttttactaa
     5461 ttcgttgtaa taatacagag atttcataga gttaatggtg cggtattgtc atcaattgca
     5521 gtgtttgttg tgaattgctt atatccatgg ctttttgtaa agcttttaaa agatcattgg
     5581 aagtatacat atcgcgaagt atgagcggct ggtaaaaata ttcacctttt gggaaaattg
     5641 ctacaaatgg aattgaacgg gcaccaaggc tttgcataag ctcttctgct aattcatttt
     5701 ttgttgtgat atctgcttta taagtatgga tgtcattgga cttaataaaa tcgatgacct
     5761 tttttgattt cagagtagtt ccttcaacca atttgcaatt agggcaccag tctgcggtaa
     5821 aaattatcat tgatacatgg gaactattat tgtgaactat cgcatcccaa tccaattgtt
     5881 tcaatggtat tctggataca gatggttttg tagtaaatag cattaaccct gatattataa
     5941 caattgccaa aagaataata aatgaagtga tacgatgtat tagcggtcgg gctatatttc
     6001 caaatatgcc ccattgccat aatcctaacg aaagcagcag aataaaccat aatgcctgcg
     6061 taagggctgt tatatcaagg agggttatca ggtaaattga agttgcaaca agcaaaaaac
     6121 ccatgcattg ttcaaaaata atcatccatt cgccaggttt agggataaaa cgaacaagtg
     6181 atggatacat agcaaggatt agatagggaa gtgacatccc tataccaata ttgataaata
     6241 tcataaagat tacgatagga tgttgtgtta atgtccatgt tagtatagct cctaaaaagg
     6301 ggccactgca gggagtagcc agaatggttg caaggactcc tttaaagtaa ctatcgatat
     6361 aagggttggc actctgcagt tttgagggaa gtgaaggaac attaaaggta tagatgtcaa
     6421 aaaaagataa agccatggaa aatataatgg cagccattac tataataaat gttttatgtt
     6481 gaaaaagttc accccagttg taaccggcaa atgctgctaa tgccgctaaa ataatgaagg
     6541 atgttataat tccaagtgtg aaaagtaaac ccatactttt tatttggcgg ggattattat
     6601 gtgcatatga tacaaaacta aatagtttca gacttattac tggtaataca catggcataa
     6661 aattaagtat aaatcctgct ataatcccaa agatgatggc atgtaatatc ccttttgatg
     6721 aataccccat atcgatagca ttgaatgtgt aattattgtg tgattgttca aggtatgtca
     6781 tttgttgcgg ttctttatat ggtgctatgt gtgattgcgg aaaatcggct gcatcgtttt
     6841 ttactttaac tttttgggcg atagttccag taatgggtgt acaggaacta tcgttgcata
     6901 gtaatgcatc aatagaaata gtaaacgaat ataacccttc acgattggtt ttgactgtaa
     6961 aagggatgca caatgaggtc tggtttttat aaatatacac cggcttgtta tctatgggtg
     7021 atatgtgcat cgttccggga agatagcggg tgtttttgaa ttctatggga tcatgggttg
     7081 tagccttagc aacaagtggt ttccctgtgc cttcgccttt agggtttgca tagatatacc
     7141 ccctcccgcc taagcgtata ttaattatag cataggcgtc agtataaggt tttacataca
     7201 gtatgtcact ttgtggctga atagtcacat cctctgcaaa agcatgaatt gtagatataa
     7261 atatactgaa tagtataatg aaaattttca caaatggttt gtatagcatg ctgttttcct
     7321 caatactata aggagatatg aaacaccctt gttggtacaa actattgata atactacttt
     7381 atttcactat ggcaattgga tgaaaaaaaa gtgtttaaag atattatact attaagaata
     7441 tagcaaaagt tgccgatatt gcaaatagaa attagtaaag gcacaaagga gggtgtccta
     7501 tgatggcgca acaaattaca gtagtagaac agagcatcga acagcttaac aatctgctaa
     7561 aaggacttgc agcagtaaga acaggattaa gtgagaagct tgtagaagtc aatgtaaaga
     7621 cacagggaag gatagaagga ttaggaacaa ttattgatag ctatcagtaa aaaaagtagt
     7681 gactatgtat ttagtatgct tctttttgac attgatatct tttacaattt ttgcaacaat
     7741 atcgctgatg atttcatggg tcaatcttaa tgttctaata aaatgataga agaaatagaa
     7801 gaacggacaa ataataccat accagaagac aatatgccac catgtgtttt gatttgtttc
     7861 ccaattatcc cctgatgcag gaacgcagtt tcatgttcag tgattttccc ttccataatt
     7921 tctctctctg ttaccgctta actttaatcc aattctacat ttgtattaat ttgtttgcaa
     7981 gtatattgtt aatattgtca cttttactat gatagttgaa aatatctaaa aatggtaaac
     8041 atcaatgtgt cccaatggta tggtaattat gatatttttt cattgaatga aatggtattg
     8101 cataaaaact tctaaaaatt agattttgac gttgccttgt tggtataaat attaattaag
     8161 atacttgatt aaattgtgaa taattgcaac ccgattatga gaggtatcaa taaattgatg
     8221 ttgacagata gtatatatac ataatatata attaatcaaa aaagggtgaa tgtgtatatg
     8281 gaaaaaaaat tattaatcat tgcagcagga ttattacttt tatgccagac tatttttgct
     8341 gctaacaaag agataaatgg aacattgggg acatcatatg ctgataaccc ctcaaagtat
     8401 ggacttgaca tttcattaca ttataattgg ttattagatc cttatttttt aacaggattg
     8461 gaatccggtt ttttctgggt tcagtgggat agaaaaattg gtgtaaagca agaaggctca
     8521 gtctatgtag acgttaaggc cgattcaaat gcatatacga ttccaataat gtttaatgct
     8581 caggtgcgat tgcccaattt gcaaagtaaa ttatatgttg tcccatttgc aacagttgga
     8641 tttggttact catttatgat attgcattat tcacgccctg attttactga ttcaagaacg
     8701 gggaagacat atgattcgga ttcaattact aaacttttta aagggctttc atgggagttg
     8761 ctttttggtg tttcattaaa acctgcaact gattccaaag ttgattttta tgctgaatgt
     8821 ggttatcgca aattgccgct gaaaagtggt gatctggaac tggatatgtc gggtttttta
     8881 cttagattcg gggtaagata tccattgtag cattagtagt gtaataatgt gtgaagtgtt
     8941 aaagttcttg actttattta tacctttatt tttagttttg taataccaat gaacatgtgg
     9001 ggttaatatg gtgcctacag catatttgtt tgatcctgtt tatatggatc atgatacagg
     9061 atggggacat cctgagcgcc ctgagcgcct ggtggcaata gatcagaaat tacgtcagca
     9121 accatactat aaatcactga ttaaagtaga gcctaaaatt ggtgcaaaac gatatattga
     9181 gctggtacat tcgccagatt atgttaagcg agtgaaagaa gagatagaat caggggtcca
     9241 ctatctggat tcaatggata ctgcagtatg taaaaggtca tatgaagttg cattgttagc
     9301 tgtaggtggc agccttaata tgtgtgatgc tataatctct ggagaggcaa tcagagggtt
     9361 ttgtgcagca agacctccgg gacaccatgc tgagtatgat tatgcggcag gtttctgcat
     9421 ttttaataat attgcaattg cagcaaaata tctccaggta gaacatggaa taaataaaat
     9481 agctattgtt gattgggatg ttcatcatgg taatggtact caacacacat ttgagcgcga
     9541 tagaacagta ttgtatataa gtacacatca gtaccctcat tatccaggaa caggagctcg
     9601 tagtgaacgg ggaaaagagg agggtgaagg ctatacattg aatttcccta tgcctgcagg
     9661 gagtggcgat actgaatata tggatgtgtt tgaaaatcat attgttccgg cacttatgaa
     9721 gtttgaacct gaaatagttt tactatcagc aggttttgat gcgcataatg ctgatccttt
     9781 atcatatata ggactttcaa ctgaagcata ctacaaattt acggttctta tcaagcaagc
     9841 tgctaatgag tacagtggcg gaagaatgat tgccttttta gaaggtggct acgatttaga
     9901 agctttagct aatagtgtag atatgatgat gagagcattc attgaggatt gaccatgctg
     9961 ttccaatgtc atgaagtttt ataaagataa actacgaatg ggtgccgcgt agtcgcccgc
    10021 ggtttggtaa gatcaccatc cagtgaagaa aatgtttggc gatagttatc ctgaatactg
    10081 aacaggctac agacaaggat tgttacaaaa tcccatggaa gaaatagatt ctggaatgag
    10141 ttcagagtgt ttgttaattc ccgaattaca atattatatg ccatttagta aatatataaa
    10201 taaaaattaa attaaataaa tattcaaaag tatgaaagca taatggggta cattttaaca
    10261 attatgttag aaattaattg taaaatattt tattaataat tttgtaaaaa agttgtacta
    10321 taaaactggt ctgtgataga attgaaacat aacgctgtta tgttcaggag ggtatatgta
    10381 tccgcttctt tttacaccaa taaccattaa tacattacag attaaaaata gaattgcata
    10441 tcctgcgtta ggattacttt attcatatga tagcttatta aatgacaggt attatgaata
    10501 tttcagggaa aaggccaaag gtggggctgg ccttgtaaca gtgggtccag tggggattga
    10561 tatagcaggt tcaggtagga tggcattatc attagctgat gatgataaaa taccgtcctt
    10621 taaaaaatta acaacaataa taaaaagtga aggagcacaa acctttgtgc aactatttca
    10681 cgctggagct tattcatatt caaaacttac tgatgatgtt caacctattg ctccatcaag
    10741 tgtatattca aaatattcaa agcaggtccc tcgtgagatg actcttgaag atatagagat
    10801 ggttcaaaca gcgtttgtaa aagctgcgtt gcgggcacag gaagcagggt ttgatggtat
    10861 tgaaataatt gcttcagcag ggtatctgat tacccagttt ctatcgccca taacaaataa
    10921 acgaacagac cgctatggag gttcttttga aaacagggtt aggtttcccc gcgaggtaat
    10981 agaaaaagta agaaaagctg ttggttttga tttccctctt ggtattagga tggcagggaa
    11041 tgattttgtt aaaggcagca atacggatag agaaactcct ctgtttgcaa aagtttatga
    11101 agatgctggc gttgatatca taaatgtaac aggtggctgg catgaagcta aaattccaca
    11161 gttgcccatg gaattaccaa ggatgggata tgcttatctg gcggctacta taaaaaaagc
    11221 agtacatgtg ccggtaatgg cttccaatag aatagctact cctgaagcag ctgaaaagat
    11281 tttgcgtgat agcatggcgg atatggttaa tctggggcgt gttttgattg cagatcctga
    11341 ttggcctaca aaagctaaag aggggaaaac aaatcttata cgcccgtgtg ttggatgcaa
    11401 tcagggctgt actgatacat tatttagcgg aatgccagta ggatgtgtat taaatgcccg
    11461 ggcaggatac gaaggtgagc gcaatataat gaaaacagat actcctctca ggattatggt
    11521 cataggtgca ggccctggtg ggttagaagc tgcagtgaga gcaaaagaag cagggcatag
    11581 ggttgaattg tatgaaatgt caactgatat tgggggacaa ttatggatag ctgcagcacc
    11641 accccataaa catgaactgc tggagattat acgatattat cgtgcaatgg tgaaacattt
    11701 ggatattcca gtattttata atactgcaat gactgtggaa ttgattaaag aaaaaaatcc
    11761 tgatcatgtc attattgcaa cgggtgctga agcggtaatg ccacctattg aagggattgg
    11821 attaccacat gtttattctg catgggatgt attagataat gatcctgtat tgggaggaaa
    11881 tgttgccgtt attggtgggg gtgcggttgg gattgaaacc gcactgttca ttgctaataa
    11941 ggggaccata tctcctgaaa ttcttcattt tttgatgttt tttgatgctg aacctgttga
    12001 ccgattgaaa gaattgatta ctcagggtac aagcaaggtt acaat
//
LOCUS       DAOOJH010000071        11811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_4982_length_11811_cov_10.787087, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000071 DAOOJH010000000
VERSION     DAOOJH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_11811_cov_10.787087"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLJ75_09205"
     CDS             <1..407
                     /locus_tag="PLJ75_09205"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05430.1"
                     /translation="PVDRLKELITQGTSKVTIIEMLPSIGKDVGRSTRWILNTELEKF
                     GVSTMTSAKVIEIANHDVIFEKDNIVQKIPVDNVVIAVGSKPVNSLLGQVEKCGIQYT
                     AIGDCKKIGKINDAIHEGFMAIVNLQKETAKI"
     gene            complement(449..2248)
                     /locus_tag="PLJ75_09210"
     CDS             complement(449..2248)
                     /locus_tag="PLJ75_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPD05431.1"
                     /translation="MNYTHYSQVFLDQVNKYGDRVAMRYKPKDKWEEITWNQFGDLVN
                     KTSKALLECGIGEQETVGIFSQNMPLWTVADIASLCIRGIPVPIYATNTAKQAEYIIN
                     DAEIKVLFVGDAEQYSKIMQILPENKYLQKIIVFKESVPIQKSEKIMYFKDFLKIGEQ
                     SNKDNMLSERMQRAKIDDLLTLIYTSGTTGEPKGVMLTHSNVIFQKGEHDLRLIDPND
                     NDVSLAFLPLSHVFERIWTYYVFATGMTNNYLEDTGKIIECIQEVKPTIMCAVPRFYE
                     KIYSTVFNQLESASPLKRKLFNWAVSVGAKHNNRRKDQQFIPPFLRLKFAIADALVLK
                     KIRSVVGGRIKFFPCAGAPLSQEIEEFFYACGIFICYGYGLTETTATVTCHEPYKFKF
                     GAVGKPLPHVEVKIDENNGEILVRGGNVMKGYYKKPQATAEVFTSDGWFRTGDAGYFE
                     ENGELRITERIKDLIKTSGGKYIAPQMIESTIGADHFIEQMAVIGDQRKYVTALIVPA
                     FDALEEYAKTHNIAFTSREDLVSKPEIIEFYQQRIEEAQKEFARFEKIKKFTLLPHEF
                     TVESGEITPTLKIKRKAIAEKYKDIIDAMYKDE"
     gene            2662..3150
                     /locus_tag="PLJ75_09215"
     CDS             2662..3150
                     /locus_tag="PLJ75_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPD05432.1"
                     /translation="MDYVKCLLLENGVGSLLDFTDSKYIKFLKSRNKYKAIEELAGVF
                     KGTDICSDIKAFVAALKEREEIMTTGIGFGIAVPHAKLESIHKIAFAIGISKKGIDFN
                     SIDGRPVHMVVLVAAGERQHKDYLKLLSKIMSLLKNDTIRVEIINAKSAKEIINILRN
                     AK"
     gene            3179..4936
                     /locus_tag="PLJ75_09220"
     CDS             3179..4936
                     /locus_tag="PLJ75_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPD05433.1"
                     /translation="MKKLHSLLKKYKCIITTFFTTRYDIREYIVQIGMALCIGAIAGL
                     GAMVFHTLIEWMRIVINPKHFADYLEIPSITIVIIPVIGGLITIGMTKMFPWQAKDRG
                     VKSIIRSVMLQNGHIPIVNTLFHLIASAVTLGTGAPLGPEGPSAKIGGGLGSYISTLL
                     RFNSRKKITYTVAGAGAAISAIFNAPIAGVFFGIEVILLNDLKNESLSALVIASVTSD
                     MISKAYLGNNSLLQIPVFQMDSTEFPYFLLLAIVCGIISLLFMGLSRSIKKLYKKIKG
                     DDVIVLLLPVSLLFAFVVFIYNDLFGIGYELINKVLSNHFPINVLIMLLVLKLLFVAL
                     YIQTGAYGGLFAPSLVIGVLVGFLFASFFSYFGVKLDPAGYAIIGMGGILAGLNSIPI
                     TAILMVFELTNDYRIILPIMLASVISHLTVTFYNKGTVYELELLEDGLDVSKSRINIL
                     ESIQVLSILKKDFQTINARTPLNQTLDKLLDSDSGLIVVDDNNEYVGILSINEVRQAL
                     LSSNIVELLICRDIVIHVEPLQKTDSAAEALKKMDKHGIDFLAVVEKNKVVGMVQHRD
                     IISIYNKLVMQLEEKAMAM"
     gene            5450..7915
                     /gene="leuS"
                     /locus_tag="PLJ75_09225"
     CDS             5450..7915
                     /gene="leuS"
                     /locus_tag="PLJ75_09225"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPD05434.1"
                     /translation="MSEAEYNPKKIEKKWHERWDESFIFQSKKDTDRNKYYILEMFPY
                     PSGRIHMGHVRNYSIGDVVARYKRMQGFNVFHPMGWDAFGLPAENAAIKHGIPPKKWT
                     DDNIDYMKKQFKMLGYSYDWNREIATHTQEYYKWNQYIFIKMYEKGLAYKRKAPVNWC
                     PTCNTVLANEQVENGVCWRCETQVTQRELEQWFFKITDYAEDLLAGHEILKEGWPERV
                     IVMQRNWIGRSTGLIVNFKVADTGEDFPIFTTRPDTIYGVTFMVIAPEHPFLERIKDQ
                     KVKDFIKRIKEQSLIERLSEEKEKEGIDTGIKIINPFNGDIVPLYVGNFVLMDYGTGA
                     IMAVPAHDTRDFAFAKKYNIPIKVVIDNPQHPVDSNTMSDAYVDDGICVSSGPFTGMH
                     NRDAIEAIVDYAEKEGIGKRKINYRLKDWLISRQRYWGCPIPIVYCEKCGAQPVDYKD
                     LPVILPVDVDFHGDSRSPLTMMPDFYNATCPVCGGSAQRETDTMDTFVDSSWYFAKFT
                     SPHSTEIFDFDEVKYWTPVDQYIGGIEHACMHLLYARFFTMVLNDLGLLQYREPFTRL
                     LTQGMVIKDGAKMSKSKGNIVDPDDSIDKYGADTVRLFMLFAAPPDKDLDWSDKGVEG
                     CYRFVNRVWRIVQKYSNCYTTYNFDTIVLDSSLKNLRNEIHRTIKAVTHDIEDRMQFN
                     TAIARMMELVNALYLADETIIQTDMGKMVVSELFDKLLPMLGPFIPHVAEELWELLGH
                     KDFIVNASWPDYIEELTMKEEIELVFQINGKIRSKCNVAANITREEMEKAALEDDRIK
                     EMIAGKTVKKVIVVPGKLVNIVI"
     gene            complement(7923..8306)
                     /locus_tag="PLJ75_09230"
     CDS             complement(7923..8306)
                     /locus_tag="PLJ75_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05435.1"
                     /translation="MYYILTFGCVAFAIGLLEIIIPQVMMSLLIKWTSSKLYSIHGII
                     LIAAGFPLTQIPSSNPLSIPLFIVGLIIVFTGPFILIYPEKVKKLFLEGLDELGHEGR
                     HLALTIDALFQMIVGIVCAVAFFLQ"
     gene            complement(8440..9081)
                     /locus_tag="PLJ75_09235"
     CDS             complement(8440..9081)
                     /locus_tag="PLJ75_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HPD05436.1"
                     /translation="MKKSFTFLFTVLFITITVFVFSQQQWKLIYEGKEDNAEIKIYTR
                     ELPGSIYKEFKGVSYADIPFNVALKVIQDYTNYHKWYGMCDALYAITVRNQQDLDMYF
                     VLDLPVVKNRDVVVKVESGWDENKGTAWVTMTSINGSEYKKDSGYVRITSLKGRWDII
                     KKDENHVEVIYQVHPELGGSLPAWIVNVAAKDQPLKTLKGVYKYSKKIIDKND"
     gene            complement(9126..10304)
                     /locus_tag="PLJ75_09240"
     CDS             complement(9126..10304)
                     /locus_tag="PLJ75_09240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPD05437.1"
                     /translation="MRECVFIDGVRTPNGRAHREKGWFRHLRPDELLTPVYNALFERN
                     SKITPDMLDAVFVGCANPSGMQNDIGRLAWLASGFPDSVPTNTLTNQCPSGMSATMHA
                     ARAIMTGEADIIIAAGVEDMEKVPMGANMDFPPRLLKYYNGADLLMGPTAEKVAELWK
                     IAREDMENMAIWSNKNAWEATVAGKFRKEIVPIKGLDDNGNEFTVDRDQWIREKIEPE
                     KMKTMMSPFKPNGVVTAATSSPLTQGACALLLMSREKADALGLSYKYKYSYGVLAGCD
                     PTIMGIGPIPAIKKLLSRTGLNLDDVGAIELNEAFASQSLACIRELKLDNENAPFKRV
                     NVWGGALALGHPLGESGARIIVTLLNVLATEFPAAKYGLASLCGAFGNAGALLVEKVN
                     "
     gene            complement(10334..>11811)
                     /locus_tag="PLJ75_09245"
     CDS             complement(10334..>11811)
                     /locus_tag="PLJ75_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPD05438.1"
                     /translation="VVKNLMKKFSLQFIIVTTIRKNNDISSFDPKQILKHKHVYELYS
                     LIQSSSGNVSPVAIDVQNDIETLLFTGGTTGVPKGCMLTHRNIYANCIQNLHALGIGG
                     LSFRGAIAAILGLPFFHSYGHCLMHTMMLFGFNLILINDSRDTKAMVDNIKKHYPVMQ
                     IGVPTQFMKLAREKLDGIGILGLSGSAPLSESTQEEFEKKSHSGIMEGYGLSEMSPVT
                     HLNTTIMYRILGGKIGVLLITLLLKIPFMLHLNNSLFRLLGTKNFGRIFTKIIAWRMK
                     STSKKTRKKSVEKRRSIGIPFPDTEIRFLDFETGNLIHIDDMLAGRRAEMLLNGPQRM
                     LGYWPKPGSGFDKDGFIHTGDVVQIDSNGYFYIVDRTKDMINVSGFKVYSKEIDEILC
                     EHPQIETAATVGIPDTEREGSEIVVVFVQPFPQFIKKLTAKDVISFLKAKVAKYAVPK
                     FVKIVDDLPRTAVEKVDKKLLRQIAIEEFSRSKRKLATKSK"
BASE COUNT         3699 a         2004 c         2209 g         3899 t
ORIGIN      
        1 aacctgttga ccgattgaaa gaattgatta ctcagggtac aagcaaggtt acaattattg
       61 aaatgctacc ttctattggt aaagatgtag gacgatcaac gcgctggatt ttaaatactg
      121 aactggaaaa atttggcgta agcacaatga catcggcaaa ggttattgaa attgccaatc
      181 atgatgtcat ctttgaaaaa gacaatattg tgcaaaaaat tccagttgat aatgttgtaa
      241 ttgctgttgg ttcaaaacca gtgaattcat tgctggggca ggttgaaaaa tgcggtattc
      301 aatatacagc aattggtgat tgcaaaaaga ttggtaagat taatgatgca attcatgaag
      361 gattcatggc tattgtaaat ttgcagaaag agacagcaaa aatataaagt ataataagga
      421 gcggtgttta tcaccgctcc ttacacattt attcatcttt atacatggca tctatgatat
      481 ccttgtattt ttcagctata gcctttcgtt taatttttag tgttggtgtt atttcacctg
      541 attctacggt aaactcatga ggaagaagcg taaatttttt aatcttctca aatcgggcaa
      601 actctttttg agcttcttca attcgttgct gataaaattc tataatctcg ggtttagaga
      661 caaggtcctc cctgctggta aaagcaatgt tatgtgtttt tgcatattct tccagtgcat
      721 caaatgctgg aacaataaga gctgttacat atttacgctg atcaccaata acagccatct
      781 gttcaataaa atgatctgca ccaatagtgc tttctatcat ttgtggtgca atatatttac
      841 ctcctgaagt ttttataaga tccttaatac gttcggtaat ccttagttca ccattttcct
      901 caaaataacc agcatccccc gttctaaacc acccatcgct ggtaaaaact tcggcagtgg
      961 cttgaggttt tttataatac cctttcatga cattaccacc gcgaacaagg atttctccgt
     1021 tattttcatc gatctttact tcaacgtgag gaaggggttt gccaacagca ccaaatttaa
     1081 atttgtaggg ttcatggcag gtaacggttg cagttgtttc ggttaatcca tagccataac
     1141 aaataaatat tccgcatgca taaaaaaatt cttcaatctc ctgcgacagt ggcgcacctg
     1201 cacaggggaa aaatttgatt ctaccaccta ccacactacg aatctttttt agcacaagtg
     1261 catcagcaat tgcaaattta agacgcaaaa aaggtggaat gaactgttgg tcctttcttc
     1321 tgttattatg ttttgcgcca actgatactg cccaattaaa taattttcgt tttaatggtg
     1381 aggcagattc aagctgattg aagactgttg aataaatctt ttcgtaaaat cgtggaacgg
     1441 cacacatgat agttggtttt acttcctgta tgcattcaat aatcttcccg gtatcttcta
     1501 agtagttatt ggtcatacca gtagcaaaga catagtatgt ccagatgcgt tcaaagacat
     1561 ggctcaacgg caaaaaggcc agagaaacat cattgtcatt ggggtcaatc aatcggagat
     1621 catgctcacc tttttgaaaa attacattgg aatgggttaa cataacacct tttggctcac
     1681 ctgttgttcc tgatgtataa attaaggtta gcagatcatc aatttttgct ctttgcattc
     1741 gttctgatag catattatct ttatttgatt gctcaccaat tttcaaaaaa tctttgaaat
     1801 acattatttt ttcagatttt tgtattggaa cactttcttt aaagacaata attttttgga
     1861 gatatttgtt ttcaggaagt atttgcatta tttttgaata ttgttcagca tcaccgacaa
     1921 atagtacttt tatctctgca tcgttgatga tatattcagc ctgtttggcc gtattggtgg
     1981 cataaatagg caccggtatg ccacggatac atagtgaagc aatatcagct actgtccata
     2041 ggggcatgtt ttgagaaaaa ataccaacgg tttcttgctc accaatacca cattcaagta
     2101 atgctttact tgttttatta actaaatcac caaattgatt ccatgttatt tcttcccatt
     2161 tatcctttgg cttataacgc atcgcaactc tatcaccgta tttattgacc tgatctaaga
     2221 atacttgaga ataatgtgtg taattcatat tgccactcct ttttatataa agttttataa
     2281 attattattt attattgttc attaagattt taacatataa aatatgataa agtcaataat
     2341 tttttttgat ttttttgaaa actttcctat ttctatatag aatagtataa acaaatactg
     2401 tctttttcaa gtgtaataat atgaaaagaa tagtttttat agatatattt catccataag
     2461 catttttaac attgcttgtt ttaatattta cctcaaccaa tcatattata aaaaatcagc
     2521 actggtcaac atgcaataat tgataatagt atccatacta aattattttt attgaaaaat
     2581 ggtagatcat agcatgatgt aagctcaata tatttagaat ttttgtaatt aagtaattga
     2641 cagggagtat tttattatgc aatggattat gtaaaatgtt tattgttgga gaatggtgtg
     2701 ggctcattgc ttgattttac agatagtaaa tatattaaat ttttaaaatc caggaataaa
     2761 tacaaggcta tagaagaact tgcaggagtt tttaaaggta ctgatatctg ttcagatatt
     2821 aaagcatttg ttgctgcact aaaagaacgt gaagagataa tgactactgg tataggattt
     2881 ggtattgcag tacctcatgc caaactggaa tctattcata aaattgcatt tgctattggt
     2941 atttctaaaa aagggataga ttttaattcc attgatggaa gaccggttca tatggtggtt
     3001 ctggtagcag caggtgaacg tcaacataaa gattatctga aattattatc aaagataatg
     3061 tcattactta aaaatgatac tattagagtg gaaataataa acgctaaaag tgctaaagaa
     3121 attattaata ttttaagaaa tgcaaaataa tgataaacac gttttataaa gccgcacgat
     3181 gaagaaattg catagtttgc tcaagaagta taagtgcatt attacaacat ttttcacaac
     3241 ccgttatgat atacgtgaat atatagtaca gatagggatg gcgctgtgta taggtgctat
     3301 tgcaggttta ggggcaatgg tatttcatac acttattgaa tggatgcgta ttgtcattaa
     3361 tcccaagcat tttgcagatt atttagaaat tccttcaatt actatagtta ttattccagt
     3421 cattggtggt ttgattacaa taggaatgac aaaaatgttc ccatggcagg caaaagatcg
     3481 tggtgtaaaa agtatcatac gatctgtaat gcttcaaaac ggacatattc caattgttaa
     3541 cacattattt catctgattg cttctgctgt tacattaggt acgggcgccc cattaggtcc
     3601 tgaggggccg tcagctaaaa ttggtggggg tttgggatca tatatttcta ctttgctacg
     3661 atttaatagc aggaaaaaaa taacatacac tgtagcaggt gcaggcgctg ctatttcagc
     3721 aatatttaat gctcccattg ctggagtttt ttttgggatt gaggttatat tgctcaatga
     3781 tctgaaaaat gaatccttga gcgcgctggt gatagcttca gtaacatctg atatgatttc
     3841 aaaagcatac ctggggaata attcattatt acagatacct gtattccaga tggattctac
     3901 tgaattccca tattttctat tactggcgat agtatgtggt attatatcat tgctattcat
     3961 gggactttct cgtagtatta agaagttata taaaaagata aaaggtgatg atgtcattgt
     4021 tctgttattg ccggtatcac ttctgtttgc ttttgttgtg ttcatatata atgatttatt
     4081 tggtattgga tatgaattga ttaataaagt gttgagtaat cacttcccaa ttaatgtgct
     4141 tataatgcta ttagtattga aactattatt tgttgcatta tatatccaaa caggtgctta
     4201 tggtggattg tttgcaccat cgttagtaat aggtgtgtta gttgggtttc tgtttgcttc
     4261 atttttttca tatttcgggg taaaacttga tcctgcagga tatgcaataa ttggtatggg
     4321 tggtatttta gcaggattaa attcaatccc aatcactgct atattgatgg tatttgaatt
     4381 gaccaatgac tatcgcatta tccttccaat tatgctggct tcggtcattt cacatttaac
     4441 cgttacattt tataataaag gcacagtcta tgaattggag cttttagagg atggactgga
     4501 tgttagcaaa agccgtatta atatacttga atcaatacag gtgttatcga ttttgaaaaa
     4561 ggacttccag actataaatg caagaacacc cttgaatcaa acattggata agcttttaga
     4621 tagcgattca ggactcattg tcgttgatga taacaatgaa tatgttggga ttttatcaat
     4681 aaatgaggta cgtcaggcgt tgctatcaag taatattgtg gaattattaa tatgccggga
     4741 tatagtaatc catgttgagc cacttcagaa aacagattcc gcagcggaag cattaaaaaa
     4801 gatggataaa catggtatag attttcttgc ggtagttgag aaaaacaagg ttgttggaat
     4861 ggtacagcat agggatataa ttagcattta taataaactt gtgatgcagt tagaagaaaa
     4921 agctatggct atgtaggttt ttcaatatat tagtgctcat tatttaagtc atgctgcaaa
     4981 atacttgacg attacggtca ttttcgtaaa atgatactat acattgagca aatttgtttt
     5041 ttggggtttt tcataaccgc agggtagctg tccatagttt ggcaagctca ccaaccacgt
     5101 cctgtaattg gacgaagtta gcatggagga aaacggttcc aaactaattt ttttaatggt
     5161 ggataagcat ttaccatagg gggaaatggt gcccaagatt aagcacaata acctatgaca
     5221 ataataatat ttttttgaga agttatccat ggtctgtctg tgaccaccca tacgggatga
     5281 taataattgg cggctgtaat cctgaataat gccatgaagt ggtttcagga tattgtttaa
     5341 ttatttggat tccggagcag gtccagaatg acaatggggg caaacgtaga tttcagggca
     5401 caaccataat gacagtagta catacttcta ttttcggagg acttaaacaa tgtcagaagc
     5461 tgaatataat ccaaaaaaga ttgagaagaa gtggcatgaa cgatgggatg aatcatttat
     5521 atttcaatcg aaaaaagata ctgacaggaa taagtattat atattagaaa tgtttcctta
     5581 tccatcaggg agaatacata tgggtcatgt gcgaaactac tctattgggg atgttgttgc
     5641 acgatataaa cgtatgcagg gatttaatgt atttcatcct atgggttggg atgcttttgg
     5701 cctgccagct gaaaatgcag ctatcaaaca tggcatacct cctaaaaaat ggactgacga
     5761 taacattgat tatatgaaga aacagtttaa aatgttgggc tattcgtatg attggaacag
     5821 agaaattgct acccatactc aagaatatta taaatggaat caatatatat ttattaagat
     5881 gtatgagaag ggacttgcct ataaacgtaa agctccggtc aattggtgcc ctacctgtaa
     5941 tacggttctg gcaaatgaac aggttgaaaa cggagtatgc tggaggtgtg aaacgcaggt
     6001 tacacaacgt gagctagagc aatggttttt taagattact gattatgctg aagatctatt
     6061 agcagggcat gaaatattga aggaaggttg gcccgaacgc gtaatagtta tgcagcgcaa
     6121 ctggataggg cgttctactg gacttattgt caatttcaag gtagctgata ctggtgagga
     6181 ttttccaatt tttaccacac gcccagatac catctatgga gttaccttta tggtgatagc
     6241 tcctgagcat ccattccttg aacgaataaa agatcagaag gtaaaggatt ttataaagcg
     6301 gattaaagaa caatcactta ttgaacgcct ttcagaagaa aaagaaaaag aaggtataga
     6361 tactggaatt aaaataatta atccttttaa tggagatatt gtgcctctgt atgttggcaa
     6421 ttttgttctt atggactatg gaacaggtgc gattatggca gtacctgctc atgatactcg
     6481 tgactttgcc tttgctaaaa aatataatat ccctattaag gttgtaatag ataatccgca
     6541 gcatcctgtt gatagcaaca ccatgagcga tgcgtatgtt gatgatggaa tatgtgttag
     6601 ttcaggcccg tttacaggta tgcataatag agatgccatc gaagcaatag tcgattatgc
     6661 agaaaaagaa ggtattggaa aaaggaaaat aaattatagg ttaaaagact ggcttatttc
     6721 tcggcagcga tactggggat gtcctatccc aattgtatat tgtgaaaagt gtggagccca
     6781 gcccgttgac tataaggatt tacctgttat tttaccagta gacgttgact tccacggaga
     6841 ttctcgttca ccacttacca tgatgcctga cttttataat gctacctgtc cagtttgtgg
     6901 tgggagtgct caacgcgaaa cggatactat ggacaccttt gttgattcgt catggtattt
     6961 tgctaagttt acttcaccgc attctacaga aatatttgat tttgatgagg taaagtattg
     7021 gacacctgtt gaccagtaca ttggcgggat tgaacatgca tgtatgcatc tcctgtatgc
     7081 acgattcttt accatggttt tgaatgattt aggattatta cagtatcgcg aaccatttac
     7141 acgattgttg acacagggaa tggtcattaa agatggcgct aagatgagta aatcaaaagg
     7201 gaatattgtg gatccagatg atagtattga taagtatggt gccgatacag ttcgtttgtt
     7261 catgcttttt gctgctccac ccgataaaga tctggactgg tcagataaag gagttgaagg
     7321 ttgctaccga tttgtcaaca gggtgtggcg tatagtacag aagtatagca attgttatac
     7381 cacatataat tttgatacta tcgtgttaga tagttctctg aaaaacttaa ggaatgaaat
     7441 ccacagaaca attaaagctg ttacccatga tattgaagat agaatgcaat ttaatacagc
     7501 tatcgcacga atgatggaac ttgtcaatgc actctatctg gcagatgaaa ctataataca
     7561 aactgatatg ggcaaaatgg ttgtgtccga actttttgac aagcttttac ctatgttagg
     7621 tccatttata cctcatgttg cagaagagtt atgggagcta ttagggcata aagattttat
     7681 tgtcaatgca tcatggccag attatattga agaattaact atgaaagaag agatagaatt
     7741 agtttttcaa attaatggga agatacggtc aaagtgtaat gttgctgcaa acataacaag
     7801 agaagaaatg gaaaaagcag cgctggaaga tgataggata aaagaaatga ttgcagggaa
     7861 aactgttaaa aaggttatag tagttcctgg taaactggta aatattgtta tataagtcag
     7921 atttattgta aaaaaaaagc tactgcacaa actattccaa caatcatctg gaatagtgca
     7981 tcgatagtaa gtgcaaggtg tctgccctcg tgtcctaatt catcaaggcc ttcaaggaaa
     8041 agttttttta ctttttccgg gtatattaat ataaatggtc cggtgaatac tataatcaac
     8101 ccaacaatga acagaggtat tgaaagtgga tttgacgatg gtatttgtgt aagtggaaat
     8161 cctgctgcaa taagtatgat gccatggata gaatataact tgcttgaagt ccatttaatc
     8221 aataatgaca tcataacttg tgggataata atttccaata accctatagc aaatgcaaca
     8281 caaccaaatg ttaaaatata atacattaac atggtctatc cttaataaat acaatatata
     8341 ggaagattaa catttggaat cataaatatg tcaagaaatt tgggagaatt aataaaccat
     8401 aagaggctta aaagctcctc ttatggttta tttactgtat cagtcatttt tatctataat
     8461 tttttttgaa tatttatata cacctttcag tgtttttaac ggttgatctt ttgcagcaac
     8521 atttactatc catgccggga gtgagccgcc taattctgga tgtacctgat atatcacctc
     8581 aacgtggttt tcatcttttt tgattatatc ccatcgaccc tttaaagaag ttattctaac
     8641 atagccactg tcctttttat attcactccc attaatagat gtcatggtta cccatgcagt
     8701 tcctttattt tcatcccagc cagattcaac cttaactact acatcccgat tttttacaac
     8761 aggaaggtcc agtacaaagt acatgtctaa atcctgttga ttccgtacag tgattgcata
     8821 taaagcatcg cacataccat accatttatg ataatttgta taatcctgaa tcacttttaa
     8881 agcaacatta aatggtatgt ctgcatagct aactccttta aattctttat agattgaacc
     8941 aggtaattcc cgtgtgtaaa ttttgatttc agcattgtct tctttacctt cgtatatcaa
     9001 tttccattgt tgttggctaa aaacaaaaac tgttatagtg ataaatagga ctgtgaataa
     9061 aaaggtaaac gattttttca tagcaaacct ccatggttta atatatttat gaaatactat
     9121 tataattaat taaccttttc taccagtaat gcacctgcat taccaaatgc tccacataat
     9181 gaagcaagcc catattttgc agcagggaat tcagttgcta aaacattaag tagtgttaca
     9241 ataatgcgtg ctccagattc cccaagagga tgacctaatg caagtgctcc tccccacaca
     9301 ttaacacgtt taaacggtgc attctcatta tcaagtttga gctcccggat gcatgctaat
     9361 gattggcttg caaatgcctc attaagttca atagcaccaa catcatctaa atttaatcct
     9421 gtccgtgaga gaagcttctt aatggctggt atgggaccaa tacccataat ggttggatca
     9481 caaccagcta aaactccata agaatatttg tacttatatg ataaaccaag ggcgtcagcc
     9541 ttctcgcgac tcatgagaag aagtgcgcat gcaccttgag ttaatggtga tgatgttgct
     9601 gctgtcacaa caccattcgg tttgaatgga gacatcattg ttttcatctt ttctggttct
     9661 attttttcgc gaatccattg atccctatca actgtaaact cattaccatt atcatccaaa
     9721 ccttttatag gtactatctc ctttctaaat ttaccagcga cagttgcttc ccatgcattt
     9781 ttgtttgacc atatcgccat attttccata tcttcacgtg caattttcca cagttcagca
     9841 actttttcag cggtggggcc catcaaaaga tctgcaccgt tgtagtattt taaaagtcgt
     9901 ggtggaaagt ccatattggc tcccatggga accttttcca tatcctccac tcctgctgct
     9961 atgataatgt cagcttcacc ggtcataatt gctcgtgcag catgcatggt tgccgacatc
    10021 cctgatggac attgattggt aagggtgttg gttggtactg aatcaggaaa tccagatgcc
    10081 agccatgcta atctcccaat atcattttgc attccagaag gattagcaca tcctacaaaa
    10141 actgcatcaa gcatatcggg agtaattttg ctattgcgtt caaataatgc attataaaca
    10201 ggtgttaaaa gctcatcagg ccttaaatgt ctaaaccaac ctttctctcg atgagccctt
    10261 ccatttggtg ttcttacacc atcaatgaaa acacattctc tcatggtaac ctctccataa
    10321 acaataaata attttattta gactttgttg ctaattttct ctttgatctg gaaaactctt
    10381 caatagctat ctggcgtaat aattttttat caactttttc tactgcggtg cggggtaaat
    10441 cgtctacaat ctttacaaat tttgggactg catattttgc tacctttgct ttaagaaatg
    10501 atataacatc tttggcagtt aattttttta taaactgcgg aaaaggctgt acaaacacaa
    10561 ctactatctc gctaccttcc ctttcagtat ctggaatgcc aactgttgct gcagtttcta
    10621 tctgaggatg ttcacacaga atttcatcta tctcttttga gtaaacttta aatccagaaa
    10681 cattgatcat atcttttgtt ctatcgacaa tgtaaaaata accattgcta tcaatctgaa
    10741 caacatcacc agtgtgtata aaaccatcct tgtcaaaacc actccctggt tttggccagt
    10801 aacccagcat gcgttgtggc ccatttaaaa gcatttcagc tcttcgtccg gcgagcatat
    10861 catcaatgtg aatcaaattt cccgtttcaa aatccaaaaa tcttatttca gtatcaggga
    10921 aaggaattcc aatgctacga cgcttttcta cagatttttt tcttgttttc tttgatgttg
    10981 atttcatcct ccatgctata attttagtaa aaatacggcc aaagtttttt gttcctagaa
    11041 gtcgaaataa actattattt aaatggagca tgaacggaat tttgagtagc agggtaataa
    11101 gtaaaacccc tattttaccg ccaagaatcc ggtacatgat ggttgtatta agatgtgtta
    11161 ccggtgacat ttcggatagt ccataccctt ccataatgcc actgtgcgat tttttttcaa
    11221 attcctcctg tgttgattcg gaaagtggag ctgaacctga aagccccaga atacctatgc
    11281 catctagttt ttcccttgct aatttcataa attgtgtggg aacgccaatt tgcattaccg
    11341 ggtagtgctt tttaatatta tctaccattg cttttgtatc acgtgagtca ttgattaaaa
    11401 ttaaattaaa cccaaatagc atcatggtat gcataagaca atgaccataa ctatggaaaa
    11461 aaggtaatcc cagaatggct gcgattgcac ctctgaacga aagacctcct atgccaagag
    11521 catgtaagtt ctgaatacaa tttgcataaa tattgcggtg cgttagcata cagcctttag
    11581 gaacaccagt tgtaccacca gtaaatagca gtgtttcaat atcattttga acatcaatgg
    11641 caacaggcga tacattgcct gatgatgatt gaataagtga ataaagctca tatacatgtt
    11701 tatgttttag gatctgtttt gggtcaaagc ttgatatatc gttgtttttg cgtattgttg
    11761 ttactataat gaattgtaag gaaaactttt tcattaaatt ttttactact g
//
LOCUS       DAOOJH010000072        11662 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5057_length_11662_cov_9.796933, whole genome shotgun sequence.
ACCESSION   DAOOJH010000072 DAOOJH010000000
VERSION     DAOOJH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_11662_cov_9.796933"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="arsM"
                     /locus_tag="PLJ75_09250"
     CDS             <1..778
                     /gene="arsM"
                     /locus_tag="PLJ75_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPD05439.1"
                     /translation="KIKNIVKQNYGKIALSKTSCCVTGSCCSSADVAKNAGYSADELA
                     KLDDANLGLGCGNPTALSSLKAGEIVLDLGSGAGIDCFIAAKKAGNSGKVIGIDMTPE
                     MIQLAKENAKKLNFHNVEFILGDIEHIPVEDNTVDIVISNCVINLASNKEQVFNEAYR
                     VLKSGGRMAISDIVLNGTLPEEIENSELAYAGCIAGAVQKEYYISSIYNAGFKNVTII
                     NERAYNTIFDNKELKIDDVQVSNLKNYSDVIKSISIVAWK"
     gene            843..1226
                     /locus_tag="PLJ75_09255"
     CDS             843..1226
                     /locus_tag="PLJ75_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPD05440.1"
                     /translation="MHVDEASKFCENWLNSWTGNQPEILITFYSDDAFYYDPTAKQGF
                     KGHEAILPYFTKLLNNNPDWKWSAEEVIPTERGFVLKWKAVIPVRIKEIVEYGVDIVE
                     VKNGKITRNEVYFDTYSLITMIKNK"
     gene            1370..2578
                     /locus_tag="PLJ75_09260"
     CDS             1370..2578
                     /locus_tag="PLJ75_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05441.1"
                     /translation="MKEGYVVPTMGKIAYGLGDLAINIAYTTIGFYLLYFLVNVAGIP
                     AQWAGLIFLIARAWDAITDPIMGMISDRTKSRFGRRRVYFLFGCIPFGLMFMLIWIVP
                     SSNQLLLFGYYTAITILFNTAFTVVAVPYNALMPELSQNYDERTSISGYRMSLSFVGN
                     LVAAVGVSFIVDVVFKGKEHYATSYPIMGVTFGIITIILIFIMFLGTRERVHSDAVVH
                     DGLIKTFKAVLSLREFRIILGMFLFNMIGFDLIQALFIFFLKDVILISEDMTFVVMGI
                     PLIVAVLSAPLWIFVSDKLDKRKAYMIAAIYFTLSLLLCLIATVNNLQFVIIIGVLAG
                     IGISASQIIPFSMIPDVIEYDEYSSGIRREGAFYGITTFLYKVASTAAIAGASAMLAY
                     FGYVENSTMA"
     gene            2612..2734
                     /locus_tag="PLJ75_09265"
     CDS             2612..2734
                     /locus_tag="PLJ75_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05442.1"
                     /translation="MAFAPGVCFLISVCFVWMFPITRERFDEIKHLLDERKKQR"
     gene            2773..3303
                     /locus_tag="PLJ75_09270"
     CDS             2773..3303
                     /locus_tag="PLJ75_09270"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HPD05443.1"
                     /translation="MKRLLSSGIIVMALISIVMVSCSRKEKGRFYSDEYHFSIVFPKT
                     WEIQQKEMGTVVIALSPEEGLKDDFRENITITTEYAGDDVTLEKYFEISQKNMKSYST
                     DYRLLYKNYTWLAKKKAMILGFTYSMSGLKLKALQYYIINNNRAYVITCTGLPHTFGR
                     YEERFLTIVRSFRFEK"
     gene            complement(3306..4130)
                     /locus_tag="PLJ75_09275"
     CDS             complement(3306..4130)
                     /locus_tag="PLJ75_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HPD05444.1"
                     /translation="MIISASRRTDIPAFYSQWFMDCIHRGSCTVKNPYNPNQIREISL
                     LPEDVEAIVFWTRYPAPLLQYLDELDGRGYHSIFLFTITGYPQWLETHSPELSQSIKI
                     FKTLSNTIGAQKVIWRYDPIVFSQELDFDFHINNFSAIASKLHHHTTRVIVSIMEPYR
                     SVIKRITKHLGDRNIVLNPFEDYNAEQMEQFFSSLSTIAHERSMHLQSCCSELSLYGI
                     SNGACIDQQLLSNIFSIQLNVPGDAHQRKNCLCAKSVDIGAYNTCRSGCLYCYASR"
     gene            complement(4149..4880)
                     /locus_tag="PLJ75_09280"
     CDS             complement(4149..4880)
                     /locus_tag="PLJ75_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPD05445.1"
                     /translation="MKIYKNIRIVYEDNHIIVVEKPAGILSQKDKTNEQSLLDIIKEY
                     IKVTYHKPGNVYAGLVHRLDKQVSGIMVFAKTSKAASRLFLQFKSHEVVKLYCAAVET
                     KITPCDNQWHLLEDSVVRIHSTTHVGVTKNAVPAALQYKVIYHDEPFSLVLVNLYTGK
                     KHQIRAQLSHHAMPIVNDTKYGAHLTTPDNQIALHACFLQFTHPTTKKVVSFYSPPYG
                     IFTVLFKELDIDSIIKQALQQENFQ"
     gene            complement(4903..5868)
                     /locus_tag="PLJ75_09285"
     CDS             complement(4903..5868)
                     /locus_tag="PLJ75_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05446.1"
                     /translation="MKKTIIIVFTLLLCIATVVMYSHFRAASFEPHYIGYLTVENSNV
                     VKQLNTILQKTNSTTIRGDKELAQCLEKQVKAVKQLALIAVTSNEYSVLIAAKKDKII
                     NQKLFDAIIADISQKKILPSSSFPQVTRYYNNSKFYFFIYDISQYTIITAYTYSLPDN
                     LILKLCLENLLIVVAFIILGTAVIIIFQQKAATKPEPEITKETKKEEKAPTANPFVSL
                     CQQHGISSVTIVQKNKKGIWKKVYSYPRNKMTVKLEEEILNELLSGTYILLNNNTVLM
                     FYNSATQLIFRLERSNPFKGKTIKEIELYITKNAEDCSNYLTKHT"
     gene            complement(5977..6561)
                     /locus_tag="PLJ75_09290"
     CDS             complement(5977..6561)
                     /locus_tag="PLJ75_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05447.1"
                     /translation="MAKTFYEVVIEGHYNFIYGLLEGYKLATGKTFIYYLSQKVNVKT
                     TTLSNVMKEWLSLKSKLQHVIIEANSWKKFEASIKKQSDGSLINIKYIKSVKKIATAA
                     FEFNFEAYAKKYGDEIKSILKSLPKSVKMVDYKPKEEIHKDARGTELYTSDHEYILKG
                     TGKLVGDIETIIDLNKKFDEHPLIEVSSITLELR"
     gene            complement(6691..8178)
                     /locus_tag="PLJ75_09295"
     CDS             complement(6691..8178)
                     /locus_tag="PLJ75_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPD05448.1"
                     /translation="MKNVKVFCTIALVIFTIVLPPVLASVCYAAETAQHEVTAESVQV
                     HGSHDIGTELPLWSALPFAGILLSIALFPLFAPHFWHHHFPKVSALWALAFAIPFVIY
                     YKGIALHEILHIYIIDYIPFIILLWALFTISGGILVRGTLKGSPMVNTIILLIGSLLA
                     SWIGTTGASMLLIRPILRSNAWRKHKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLH
                     GVPFFWTLTHLFTVWGFALAILLALYFIIDSYFYSKEDKSKEEYGEKKALKVEGWHNF
                     IFLAGVVGGVLFSGIVKLGEVNILGVHQTIENFIRDAVLIIMGLLSLKTTTQQIRKDN
                     EFSWFPIQEVAYLFAGIFMTIIPALAILKAGEHGALAFLINAVKEPAHYFWATGSLSS
                     FLDNAPTYLTFFNTALGSFYAGMPEIDAVHRLIAEQNIYLQAIAAGAVFMGANTYIGN
                     APNFMVKSISEEAGIKMPSFFGYMFKFSIPILIPVFILVTYVFFR"
     gene            8708..10015
                     /locus_tag="PLJ75_09300"
     CDS             8708..10015
                     /locus_tag="PLJ75_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPD05449.1"
                     /translation="MKTLHKIIIGDSRQIKDVPDESVHLIITSPPYWQLKDYGNGKQI
                     GFNDTYEEYINNLNLVWNQCQRILHKGCRLCVNIGDQFARSVYYGRYKIIPIRTEIIK
                     FCESAGFDYMGAIIWQKVTTCNTTGGATVMGSFPYPRNGIIKLDYEFILIFKKYGNQP
                     EVSNVIKEQSKLTREEWNQYFAGHWNFPGVKQKKHLAMFPEELPGRLIKMFSFIGDTV
                     FDPFLGSGTTSLAAKNLNRNSIGYEINEDFLPIIKEKLDVKQGSIFQDITFEITKQDE
                     CKGDFKEEIKKLPYIFKDPIKFDKKIDPRKLRFGSKIDSTRSEHKTSEHENYLTVKEI
                     ISPEILILNNGLKIRLLGVKENPEKKGDAVQLLREKTRGKKIFLKFDTTIKYDDNNTL
                     LCYLYLFNKTFLNAHLIKNGLVDVDTAFDYKYRSKFLSERIAT"
     gene            10044..10571
                     /locus_tag="PLJ75_09305"
     CDS             10044..10571
                     /locus_tag="PLJ75_09305"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MjaI family restriction endonuclease"
                     /protein_id="HPD05450.1"
                     /translation="MDEWREYYFKNVRTKEHIEELGRKLYIKITEVIQAEVAEITEED
                     CIKYMLQMVIDRTFDGYMTEIQTIYGQLEKILNVKIEPAPDEWDRLFNIDFFIKIRDK
                     FIGLQIKPINSGIQLPEIFKEKAIQEETHKQFTEGFGGKVFYIYSVKAGEKKEIYNKE
                     VIEEIRKEIKKLEKE"
     gene            complement(10921..11322)
                     /locus_tag="PLJ75_09310"
     CDS             complement(10921..11322)
                     /locus_tag="PLJ75_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPD05451.1"
                     /translation="MSYLIDTNIIIYSLKGDSVVHEYFLKNENIPKAISVITYGELLF
                     GAKKSQNYEKNLAIVYRIKELFPIIDIDKAIIETSSELKVNMQKIGSPIDDFDLLIAS
                     TALTMNSILVTNNEKHFNRIKGLKIENWSRK"
     gene            complement(11306..11584)
                     /locus_tag="PLJ75_09315"
     CDS             complement(11306..11584)
                     /locus_tag="PLJ75_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HPD05452.1"
                     /translation="MAILQVRDIDDRLYASLKAIAKSQNRSISQEVISIIEKYLSNPA
                     VYKSNPTREFIALSGAWEDDRSADEIIDSIKKSRKNSERFKKEHVIFD"
BASE COUNT         3792 a         1826 c         2157 g         3887 t
ORIGIN      
        1 aaaaataaaa aacattgtta agcaaaatta tgggaagatt gcattatcta aaacttcatg
       61 ttgtgttacc ggtagttgtt gttcatctgc tgatgttgct aaaaatgccg ggtattctgc
      121 tgatgaactt gctaaacttg atgatgccaa tttagggctt ggatgtggta atccaaccgc
      181 tttatcttca ctgaaagccg gtgagattgt tcttgattta ggaagcggtg ctggcattga
      241 ttgtttcata gcagcaaaga aagcaggcaa cagtggtaaa gtcattggta ttgatatgac
      301 acctgagatg atccaattag caaaggaaaa tgcaaaaaaa cttaattttc ataatgtaga
      361 atttatattg ggagatattg agcatatacc ggttgaggat aatactgttg atatcgttat
      421 atccaattgt gtaatcaatt tagcatcaaa taaagagcag gtgtttaacg aagcataccg
      481 tgtgttaaaa agtggtggca gaatggcaat atcggatatt gtattaaatg gaacattacc
      541 ggaagagatt gaaaactcag aattagcata tgcgggatgt attgctggtg ccgttcaaaa
      601 ggagtattat atctcatcga tatataatgc tggatttaaa aatgttacca ttatcaacga
      661 acgtgcttac aacactattt ttgataataa agaattaaaa atagatgatg ttcaagtatc
      721 aaatttgaag aattatagtg atgtaattaa aagcataagt attgtagcat ggaaataggt
      781 ttactaaatg ttagatcagc atggttgcta tatttcttaa cgtattttaa ttggaggggc
      841 aaatgcacgt agatgaagct tcaaaatttt gtgaaaattg gcttaattca tggactggga
      901 atcaaccaga aatattaata actttttatt ctgatgatgc attctattat gacccaactg
      961 caaaacaagg attcaaaggg catgaagcaa tattacctta ttttacaaag ctattgaaca
     1021 ataatcctga ttggaaatgg tctgctgaag aagttatacc cactgagaga ggttttgttt
     1081 taaaatggaa agcggtaata ccagtgcgga taaaagaaat agttgaatat ggtgtagata
     1141 ttgttgaagt taaaaatggt aaaattacca ggaatgaagt ttattttgat acatatagtt
     1201 taataactat gatcaaaaat aaatagttta ttattcaatc ctataaaaag cattaataat
     1261 ttatactatt atcataaatt tccttgcatt ttggctatag atattatcta attccctcaa
     1321 tatatttttg ttacaatcat tattgttatc aatttttgga tggtaagcca tgaaagaagg
     1381 atatgtagtt cccaccatgg gaaaaattgc ttatggattg ggtgatttag ccatcaatat
     1441 tgcctataca acaattggtt tttatctttt atattttctg gttaatgttg ctggaattcc
     1501 tgcacagtgg gcaggtttga tctttttaat tgctcgggcg tgggatgcta tcaccgaccc
     1561 tattatgggg atgatatctg acaggacaaa atcaagattt ggcagacgac gtgtgtattt
     1621 tttgtttggg tgtattccgt ttggactcat gttcatgtta atatggattg ttccatccag
     1681 caatcaacta ctattgtttg gatattatac tgctattacc atactgttta ataccgcttt
     1741 caccgttgtt gctgttcctt acaatgcact gatgccggag ctatcgcaaa actatgatga
     1801 acggacaagt atatccggct atcgcatgtc actttcattt gttggtaatc tggttgccgc
     1861 tgtaggtgtg tcgtttatcg ttgatgttgt cttcaaaggt aaggagcatt atgcaaccag
     1921 ctatcctatt atgggagtta cttttggtat tattaccata attttaattt tcattatgtt
     1981 tttgggaacc agagagcggg ttcactctga tgcagtggtg catgatggac ttatcaaaac
     2041 atttaaagct gtattgagcc ttcgtgaatt cagaataata ctcgggatgt ttttattcaa
     2101 catgattggt tttgatctta ttcaggcgct tttcatcttc tttttaaaag atgtaattct
     2161 tatttctgaa gatatgacgt ttgtggttat gggtattcct ttgattgtag cggtactttc
     2221 agctccattg tggatttttg tttcggataa gcttgataaa cgcaaagcct atatgattgc
     2281 tgctatttat tttacacttt cattattatt atgtttgata gctacggtaa acaatctgca
     2341 gtttgtaatc atcattggtg tactggcagg tattggcatt tctgcatcgc agattattcc
     2401 tttttctatg atacctgatg tcattgaata tgatgaatat tccagtggca tccgtcgcga
     2461 aggagctttt tatgggatta caacattttt gtataaggtt gcatcaacag cggcaattgc
     2521 gggagcttca gctatgctgg catattttgg ctatgtagaa aacagcacca tggcatagcc
     2581 acagtcggca attagtgcca tacgatacat gatggctttt gcaccgggcg tatgcttttt
     2641 aatttcggtg tgttttgtct ggatgttccc cattacccgt gaacgttttg atgaaataaa
     2701 gcatttactt gatgaacgaa agaagcaacg atgatgcaat gatgcgttaa tattttatgt
     2761 ctggtggaga ttatgaagcg cttactatca tcaggaatta ttgtcatggc acttatcagt
     2821 atagtcatgg tatcctgctc caggaaagag aaggggcgtt tttatagtga tgaataccat
     2881 ttttcaatag tattcccaaa aacatgggag atacagcaaa aagagatggg taccgttgtt
     2941 atagctctta gtcctgaaga aggattaaaa gatgatttcc gcgaaaacat aaccataaca
     3001 acagagtatg ctggtgatga tgtaaccctt gaaaaatatt ttgaaatatc acaaaagaac
     3061 atgaaaagct attctacaga ttacaggctt ttatataaaa actatacatg gcttgctaaa
     3121 aagaaagcta tgatactggg atttacctat tcaatgagtg gcttaaagct caaagcattg
     3181 caatattata tcattaataa taacagagca tacgtcatta cctgcaccgg attgccacat
     3241 acctttggac gctatgaaga acgtttttta acaattgtac gttcgttcag atttgaaaaa
     3301 taaaatcacc tgctggcata acaatataaa catccagacc tgcaggtatt atatgctcct
     3361 atgtcgacac tttttgcaca cagacagttt ttacgctgat gagcatctcc gggtacatta
     3421 agttgaatgc taaaaatatt cgatagcaac tgctgatcta tgcacgctcc attacttatt
     3481 ccatacagtg aaagctcgct gcaacagctc tgtaggtgca tgcttcgctc gtgtgcaatg
     3541 gttgatagcg aagaaaaaaa ctgttccatc tgttctgcgt tataatcttc aaagggattt
     3601 aatactatat ttctatcgcc aagatgtttg gtaatgcgtt taatcacgct cctgtaaggc
     3661 tccatgatgc tgacaattac tcgtgtggta tggtggtgca gtttggatgc tatagcactg
     3721 aagttgttga tatgaaaatc aaaatccaat tcctgtgaaa aaactattgg gtcataccgc
     3781 catataacct tttgcgctcc aattgtgttg cttaacgttt taaagatttt tatgctctgc
     3841 gatagttccg gtgaatgagt ttcaagccat tgaggatagc cggtgatggt aaataaaaat
     3901 atagagtgat atcccctgcc atccagttca tcaaggtatt gcaatagcgg ggcaggatag
     3961 cgagtccaga atactatcgc ctcaacatct tcgggcagta gcgatatctc ccttatttga
     4021 ttgggattgt atggattttt taccgtacaa ctgcccctgt gtatgcaatc cataaaccac
     4081 tgcgaataaa aggcagggat gtctgttcgc cggctggcac ttattatcat gagctatcgc
     4141 caaaactatt attgaaaatt ttcttgctgt aatgcttgtt tgataatgct gtcaatgtca
     4201 agctctttga atagcacggt gaagattccg tatggtggac tataaaacga taccaccttt
     4261 tttgtggtag ggtgggtaaa ttgtaaaaag catgcatgaa gtgcaatctg attgtccggg
     4321 gtggtgaggt gtgcaccata ttttgtatca ttaacaatgg gcatagcgtg atgtgaaagc
     4381 tgtgcgcgta tctgatgttt tttgccggta taaaggttaa ccagaacaag cgaaaacggt
     4441 tcatcatgat atataacctt atactgcagt gctgctggta cagcgttttt agtaacacca
     4501 acatgtgttg tactgtgaat gcggacaaca ctgtcctcaa gaagatgcca ctggttatcg
     4561 cacggagtaa tttttgtttc aacagcagcg cagtatagtt ttacaacctc atgactttta
     4621 aactgcaaaa atagccgtga tgcggctttt gatgtttttg caaataccat aatccctgaa
     4681 acctgtttat ccaatcggtg aacaagtcct gcataaacat tgccgggttt gtggtaagtg
     4741 actttaatat attccttaat tatgtccagc aacgactgct cgtttgtttt atctttttga
     4801 cttaagatcc ctgctggttt ttcaacaacg ataatatgat tgtcttcgta aacaatgcgg
     4861 atatttttat atattttcat gatgacaatg atgcagcgtg ttttatgtat gttttgttaa
     4921 ataattgcta caatcttcag cattttttgt gatgtacagt tcaatctctt ttatggtttt
     4981 tcctttaaaa ggattgctcc tttcaagtct aaaaattaat tgagtagcgc tattgtaaaa
     5041 catcaatacc gtattgttat ttaaaagaat atatgttcca cttaatagtt cgttaaggat
     5101 ttcttcttca agctttaccg tcattttatt tctgggatag ctataaactt ttttccatat
     5161 ccccttttta tttttttgta cgatagttac tgatgatatg ccatgctgct ggcataatga
     5221 aacaaaggga ttggcagtgg gagccttttc ttcttttttt gtttcttttg ttatttcagg
     5281 ttccggtttt gttgccgcct tttgctgaaa gatgatgatt acagccgtgc ctaaaataat
     5341 gaatgcaacc acaatgagaa gattttcaag gcacaattta agaataagat tatctggcag
     5401 tgaataggta tatgctgtaa ttatggtata ttgtgaaata tcataaatga agaagtagaa
     5461 tttactgttg ttataatagc gtgttacctg tggaaatgat gacgatggca gtattttttt
     5521 ttgtgagata tcagcaatga tagcatcaaa taatttttgg ttaatgatct tatctttttt
     5581 tgctgcaatt aaaacgctgt attcattact ggttacagca ataagggcaa gctgtttaac
     5641 ggctttaacc tgtttttcca gacattgagc taattcttta tcaccgcgga ttgttgtgct
     5701 gtttgttttt tgaagtatgg tattcaactg ttttacaaca ttactgtttt caactgttaa
     5761 atatcctatg taatgaggtt caaaggatgc agccctgaaa tgggaataca tcacaacagt
     5821 tgcaatgcat agcaatagag taaatacaat tataattgtt tttttcatag atagttgtat
     5881 taaacaaacc ttaatgataa ataattacaa tatatcatat ctgtattcaa tatcggaaaa
     5941 ttatatcaca atactgaata cagagagttg ttttgtttat cttaattcta atgttattga
     6001 tgatacttca atgagcggat gttcatcaaa ctttttatta aggtcaataa ttgtttcaat
     6061 atcaccaaca agttttccag tgccttttag tatatattca tgatcagaag tatatagctc
     6121 tgttcctctg gcatctttat ggatttcttc tttgggttta taatctacca ttttaactga
     6181 ctttggtaac gatttcaaaa tgctttttat ctcatcgcca tatttttttg cataggcttc
     6241 aaaattaaat tcaaatgcag cagtagcaat cttttttacc gatttaatat actttatgtt
     6301 gattagtgaa ccatcagatt gtttttttat agatgcttca aactttttcc atgaatttgc
     6361 ttcaataatc acatgttgca attttgattt tagtgaaagc cattctttca ttacattaga
     6421 caatgttgta gtcttaacgt ttaccttctg gcttaaataa taaataaaag ttttacctgt
     6481 agctaactta taaccttcaa gcaaaccata aataaagtta taatgtcctt ctatcactac
     6541 ttcataaaat gtttttgcca taataacacc tcttataata attaaatttt aaaatgtaaa
     6601 acaatgcaca ttataatgca gttaaaacac taattacact tatcaatgag ggcgatgaaa
     6661 tcgccctcat ctctatctat taattaatat ctatcgaaag aatacatatg tgacaagtat
     6721 aaaaacagga ataagaattg gaattgagaa tttaaacata tagccaaaga aacttggcat
     6781 cttaattcct gcttcttcgg atatggattt taccataaag tttggcgcat tcccaatgta
     6841 ggtgttggca cccataaata cagcccccgc agctattgcc tgcaggtata tattttgttc
     6901 ggcaatgagc ctgtgtacag cgtctatctc gggcatgcct gcataaaaac tccctaatgc
     6961 agtgttgaaa aatgttaaat aggttggtgc attgtcaagg aagctggaga gtgaacctgt
     7021 tgcccagaag taatgcgctg gttctttaac agcgttgatc aagaatgcca atgcgccatg
     7081 ctcaccagct tttaatattg ccagtgcagg aatgattgtc ataaatatcc ctgcaaataa
     7141 atacgcaacc tcttgaattg gaaaccatga aaattcattg tcctttctta tttgttgtgt
     7201 agttgttttt agtgatagta accccataat tatgagtact gcatcacgta taaaattttc
     7261 tatagtttga tgcacaccca atatatttac ttcgcctaac ttaacaatgc cactgaataa
     7321 aacaccacca acaacaccag ctaaaaagat aaagttatgc catccttcaa cttttaatgc
     7381 tttcttttcg ccatattctt cttttgattt atcttcttta ctgtaaaagt atgaatcaat
     7441 gataaagtac aaagctaaaa ggatagctaa tgcaaatccc catactgtaa atagatgagt
     7501 taatgtccag aagaacggca cgccgtgtaa aaaacctaaa aatagtggcg ggtcacccaa
     7561 tggtgttaat gaacctccaa tattgcttac taaaaatata aaaaatacta ttgtgtgtac
     7621 tttatgtttg cgccatgcat tggagcgcaa tatggggcgt attaacagca ttgaagcgcc
     7681 ggttgttcca atccatgaag ccaataaaga acctatgagc aatatgattg tatttaccat
     7741 aggtgaaccc tttaaagttc cacggaccag aataccacct gatattgtaa aaagtgccca
     7801 tagcaagatg ataaatggga tataatcaat tatataaatg tgcaaaatct catgtaatgc
     7861 tatcccctta tagtaaataa caaaaggaat ggcaaatgcc agtgcccaca aagctgatac
     7921 cttggggaaa tgatgatgcc agaaatgagg cgcaaacagt gggaataaag ctattgatag
     7981 taatattcct gcaaatggta gggctgacca caatggcaat tcagtgccaa tatcatggct
     8041 gccatgaact tgcacagatt cagcagtcac ttcatgctgt gctgtttcag cagcataaca
     8101 taccgatgca agcacaggtg gcagtacaat tgtaaaaatt acaagagcta tcgtacaaaa
     8161 cacttttaca tttttcatgc cgatctccat tctataattt atgtattatt gtggcaataa
     8221 ttataatacg tatattattg tcaaataaaa tactgagttt atttttattg aacggatgat
     8281 gagcattttt atgggaagga gtttgtgata tgccaaaaaa agctttgtat atctaaacta
     8341 ttgtttagct ggataaattg aataatgaaa aatccacttg acgaaattat agaaagggtt
     8401 acagttttca gcaaaacaca gatggtgata tatactaaat taagtatgta ttatcatgca
     8461 atttttatga taaagacaaa gtaaggtttg tgggggaagg agtcaggcga tatcgcaatc
     8521 atgcaaacta aaatgaataa atgcttcata tactaaatca gcaaatgggg tactagttga
     8581 gcaggcgttc aggccgttta gcctgacata gattgtatta taacctgctt tgcagaatta
     8641 gattacactt ataaatatca ggtgaaacgg cggctttaaa caagaaaaac tattttaagg
     8701 atatacaatg aaaacgcttc ataaaataat aatcggtgat tcaaggcaga taaaagatgt
     8761 tccggatgaa tcagttcatc tcattattac atcaccccct tattggcagt taaaagatta
     8821 tgggaatggg aagcagatag gttttaatga tacctatgag gaatatatta ataatctcaa
     8881 tttggtgtgg aaccaatgtc aaaggatttt acataaaggt tgccgtttgt gtgtcaatat
     8941 tggcgaccaa tttgctcgtt cagtttatta tggaagatac aaaattattc caataagaac
     9001 agaaatcatc aagttttgtg aaagtgcagg gtttgattat atgggcgcca tcatatggca
     9061 gaaagttact acctgtaata ctactggagg agcaacagta atgggttctt ttccttatcc
     9121 aaggaatgga ataattaaac tggattatga atttatttta atttttaaaa aatatggaaa
     9181 tcagcctgaa gtgagtaatg taattaaaga acaatcaaag ttgaccaggg aagagtggaa
     9241 tcaatatttt gcagggcatt ggaattttcc tggtgtaaaa cagaaaaaac atctagcaat
     9301 gtttcccgaa gaattaccag gacggcttat taaaatgttt agttttattg gagatactgt
     9361 ttttgaccct tttctgggaa gcggaactac ttctttagcg gctaaaaatt tgaatcgaaa
     9421 ttctattgga tatgaaataa atgaagactt tttacccatc ataaaagaaa aacttgatgt
     9481 aaagcagggg tcaatatttc aagatataac ttttgagata actaaacagg atgaatgcaa
     9541 aggagacttt aaagaggaga taaaaaaatt accttatatt tttaaagatc ctataaaatt
     9601 tgataagaaa attgacccga gaaaattaag gtttggttct aaaatagaca gtactcgttc
     9661 tgaacataaa acttctgaac atgaaaacta cttaactgta aaagaaataa tttcaccaga
     9721 aatattgata ttaaataatg gattaaaaat acggttatta ggagtaaaag aaaacccgga
     9781 aaaaaaagga gatgctgttc aattattaag agaaaaaacc agaggaaaaa aaattttttt
     9841 aaagtttgat actactataa aatatgatga taataatact ttactttgct atctctattt
     9901 atttaataag acatttttaa atgcacattt gattaaaaat ggtttagttg atgttgatac
     9961 agcatttgat tacaagtaca ggtcaaaatt tttatcagaa aggattgcaa cataatggcc
    10021 aaagaatgga tatttaatag tgtattgatg aatggaggga atattatttc aaaaatgtac
    10081 gaactaaaga acacatcgaa gaacttggta gaaaactgta tataaaaata acagaagtta
    10141 ttcaagcaga agttgctgaa atcacggaag aagattgtat taaatatatg cttcagatgg
    10201 taattgaccg aacctttgat ggttacatga ctgaaataca aactatatat ggacaacttg
    10261 aaaaaatctt aaatgtaaag attgaaccag caccagatga atgggatagg ttatttaata
    10321 tagatttctt cataaaaata agagacaaat ttattggact acagataaaa cctataaata
    10381 gcggcataca attgcctgaa attttcaaag aaaaagccat acaagaagaa acacacaaac
    10441 aatttactga aggttttggt gggaaggtct tttacattta ttcggtaaaa gcaggagaga
    10501 aaaaggaaat atataacaaa gaagttatcg aagaaatcag aaaagaaatt aaaaaattag
    10561 aaaaggaatg aattgggttg cgctatatca caaatttata taatgattta attcatgaag
    10621 tttaccgtga agaatatata ctttttgttg tgaaaatatt gtcaggatat tttccatgtg
    10681 tcttatcaaa tgttctggct gatcttaatt tatcaacatc gctcagttct gtgtaactaa
    10741 cagaatgcca tgagttactg aagtttttga aaataaaacg aactttatat ttatttgcaa
    10801 ttgtgcgcat catcttgctt acacgacgcc gcagatcatg acgcgatgtg gcgcagaccg
    10861 aacgagcgac agtgagtgaa gcccataagc tttgttatat gcagtgccag ttatttatac
    10921 ttacttcctg gaccagtttt ctatttttaa tcctttaatt ctattaaaat gtttttcatt
    10981 atttgtgact aaaattgaat tcatggtcag cgctgttgat gctattaata aatcaaaatc
    11041 atctattgga gaacctatct tttgcatatt tacttttaat tcactggaag tttcaatgat
    11101 agctttatct atatcgatga ttgggaataa ttcttttatc cgataaacta tcgctaaatt
    11161 cttttcataa ttttgggatt ttttagcacc aaataacaat tctccatagg tgattaccga
    11221 tattgctttt ggaatattct cattttttaa aaaatactca tgaacaaccg aatcaccctt
    11281 aaggctataa ataattatgt ttgtgtcaat caaatatgac atgttctttt ttaaaccttt
    11341 cagagttttt acgtgatttt tttatcgaat caattatttc atctgcactt cgatcatctt
    11401 cccatgcacc agaaagtgct ataaattctc ttgttggatt acttttataa accgccggat
    11461 ttgaaagata cttttcaata atagaaatta cttcctggct tatagatctg ttttggcttt
    11521 ttgcaatagc ttttagtgaa gcatataatc tatcatcaat atcgcgaact tgtagaattg
    11581 ccataatatc acctccattg cagataatat agttaatatt catgaaaagt caagcatttt
    11641 tcatgaaaaa cgggcatttc at
//
LOCUS       DAOOJH010000073        11575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5112_length_11575_cov_9.705816, whole genome shotgun sequence.
ACCESSION   DAOOJH010000073 DAOOJH010000000
VERSION     DAOOJH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5112_length_11575_cov_9.705816"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLJ75_09320"
     CDS             complement(<1..488)
                     /locus_tag="PLJ75_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05453.1"
                     /translation="MKRVEKNQQTRAKIIEAARTLFVQQSYSKTTIRQILKKTGITTG
                     SLYHFFKNKESILRQITADYLLDTDKIIRSFMKEYDPMVHYILIIYIQLKLIDSNPNL
                     ADIWQHTYSLWSITEMVCTKSAKWNKMMFGKYNQSLTDDDWYARSMAINSLIHYITIE
                     KI"
     gene            complement(709..1287)
                     /locus_tag="PLJ75_09325"
     CDS             complement(709..1287)
                     /locus_tag="PLJ75_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPD05454.1"
                     /translation="MAKSVKGTKTEKNLLAAFAGESQARTRYTFFSSVAKKEGFEQIS
                     ALFLETAENEKEHAKRFFSYLEGGMVEIQAAYPAGVIGTTLENLKAAAAGENEEHTKL
                     YPESAKIAEEEGFPEIAFLFRKVAEVEKHHEQRYLDLAKNIEQGKVFKKEQKVQWKCR
                     NCGYIHEGNSAPEKCPTCQHPQSYFELLCDNY"
     gene            complement(1315..1692)
                     /locus_tag="PLJ75_09330"
     CDS             complement(1315..1692)
                     /locus_tag="PLJ75_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPD05455.1"
                     /translation="MATKQLEVYKCDICGNIVEVLHEGKGELVCCGQPMKLQVENTVD
                     ASKEKHVPVAEKTSDGIKVQVGSVEHPMEEKHYIEWIQLIAGSKSYRQFLKPGEKPVA
                     IFKVDTASFAVREYCNLHGLWKA"
     gene            complement(1790..2779)
                     /gene="cydB"
                     /locus_tag="PLJ75_09335"
     CDS             complement(1790..2779)
                     /gene="cydB"
                     /locus_tag="PLJ75_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPD05456.1"
                     /translation="MDVNIFQNIWFIIIGFLLMVYSILDGFDLGIASLMPVLAKDENE
                     QKKVMDSIWPVWDGNEVWLITAGASLFAAFPFAYATVFSGFYLALMLVLFALIFRAVS
                     MEFWYYDEKRRKIWATAFTAGSLLPALLFGVALGNVICGIPIDINMNFTGNFFTLLRP
                     FPLVIGLLGLSAILLQGCTWAIHKIEGEVAKRAHSILNKLLIANAVFFILAIVLTIIF
                     LPDILSSSIGWIGAIVYAVALSYVYLSNKHKRAVNALRGSSFAFIALWIMAGAALYPN
                     LVRATIGAYNITIVNASSTVYTLKIMSIIALIGMPIVIAYTFYVYKVFKGKVS"
     gene            complement(2799..4115)
                     /locus_tag="PLJ75_09340"
     CDS             complement(2799..4115)
                     /locus_tag="PLJ75_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPD05457.1"
                     /translation="MDVVMLSRIQFGLAAGVHFIFPSLTLGLTLLVLIYEYKFYKTDD
                     DIFKTISSFFIKILGLVFALGVATGIVLEFSFGTNWAEYSRMVGDIFGAPLAAEGIFA
                     FFLESVFLGVLLFARNRVSKKVYLLSAFLVFFGSHLSGLWIIIANSWMQTPAGFAIEG
                     GRAVLTNFWAAALNPSTLVRFAHVIIAGWITGSLFAAGIAAWYLIKRLHTNEAQHILK
                     TALIIFCIMSLAQFASGHIHSVQVTRIQPEKMATFEALWQTQQGAPMSIIGIPVESQK
                     KTYYEIKIPKLLSLLAYFDPNAEVKGRDAFPDDELPPVVLPYFSYHIMIGLGSLFALI
                     GIISIVLLLTKKLFNARWFWYVLFFAIPLPIIANEFGWIAAEVGRQPWAVYKVLKTAD
                     AVSIIVPASHVAFSLVILSLIYLMLIVMFIMILTKIIKKGPQSEIY"
     gene            complement(4194..4358)
                     /locus_tag="PLJ75_09345"
     CDS             complement(4194..4358)
                     /locus_tag="PLJ75_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPD05458.1"
                     /translation="MGKKYECTVCGYIYDPEEGDPDNGIQAGTAFKDLPDDWVCPICG
                     ATKEQFEPLD"
     gene            complement(4480..4677)
                     /locus_tag="PLJ75_09350"
     CDS             complement(4480..4677)
                     /locus_tag="PLJ75_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HPD05459.1"
                     /translation="MYTFTVHDMMCNHCIMHITQSIQAIDKSSKVQCNLDSKTVTVDS
                     SLDINQIIKAIKDAGYTPVAQ"
     gene            complement(4979..5383)
                     /locus_tag="PLJ75_09355"
     CDS             complement(4979..5383)
                     /locus_tag="PLJ75_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPD05460.1"
                     /translation="MEQYKHYLESKGISPSYQRLKILEYLDQNRSHPTVDEIYSALLP
                     LIPTLSKTTVYNTVSLFVQQGLVQMLLLDNVEARYDITVMPHAHLTCSQCGNVFDIDS
                     TPYVKFPQTIEGHTIEGYQIALRGICKNCLAD"
     gene            complement(5449..6624)
                     /locus_tag="PLJ75_09360"
     CDS             complement(5449..6624)
                     /locus_tag="PLJ75_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPD05461.1"
                     /translation="MKPIALNDFIYWVGGIDWDLRNFHGYLTQRGSTYNAYLIMDEKI
                     TLIDTVKYYLMDEMLERISCIVDPSKIDIIISNHVEMDHSGGLPQLMELCPNAMLITS
                     PNGAKGLEAHYKKNWNYTVMKTGDSISLGKTSLQFILEQMVHWPDNMACYMPETKILF
                     SNDAFGQHIATSERFDTEYPLDIIMNEAKKYYANIVLPYDKQTQKVLDAVSTLDIAMI
                     APSHGILWKDHVQAIVTMYRSWAANKTTNKALIIYDTMWNSTKLIAHAIKNAFEEKNI
                     PVQLYDLKTIHISDIMTEVLDAHYICVGSPTLNNNMLPTVAAFLTYCKGLAPKNRVGL
                     AFGSYGWGGQSVAQVEDVLQQMGWRLLPAIKVNYVPQKQLLVTIAQEVYEKINEIDF"
     gene            6958..8250
                     /gene="eno"
                     /locus_tag="PLJ75_09365"
     CDS             6958..8250
                     /gene="eno"
                     /locus_tag="PLJ75_09365"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPD05462.1"
                     /translation="MTIITDVHAREILDSRGNPTVEVDVELSSGIAGRFAVPSGASTG
                     EHEAIELRDGDKARYNGKGVLKAVQNVNEIISGAVVEMDALRQIEIDKTLIDLDGTPN
                     KAKLGANAILGVSMACAKAVAETLGMPLYQYIGGVGACELPVPMMNILNGGKHADNNV
                     DIQEFMIVPVGTDSFKEALRMGVETFHALKKVLTKKGYNTGVGDEGGFAPNLKSNAEA
                     FEVILEAIKEAGFKAGQDIYLAIDAAASEFYKNGKYVMAAEEKPEKTSEEMVNVYLEW
                     INKYPIISIEDGMAENDWDGWKLLTDKAKNTVQLVGDDVFVTNTSIFQKGIEKKVANS
                     ILIKLNQIGTLTETIEAIEMAKRAGYTAVISHRSGETEDPAIADLAVAMNTGLIKTGS
                     ASRTDRLAKYNQLLRIEEQLGVFAKFNGRRVFYSIGKP"
     gene            8213..8671
                     /locus_tag="PLJ75_09370"
     CDS             8213..8671
                     /locus_tag="PLJ75_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05463.1"
                     /translation="MAEGYFIPLVNHNTKFTLFIVILILFSVYFFVLSDSGILKRVIL
                     KHDIQHIQDQIALKQQQINKINRLDNNKMYSMYMLQESINAGYIPKGAKVYQFKDIPD
                     STKHREDSLFQTQHEKYLQYIQYGRIFWITFSALIMLGVLLHHRRKPVVL"
     gene            8682..9239
                     /locus_tag="PLJ75_09375"
     CDS             8682..9239
                     /locus_tag="PLJ75_09375"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HPD05464.1"
                     /translation="MIPYNSKLNQRDLQVLQWQLKGTTDAVSGIFEKCNFGFPQIILL
                     SPIVNDLIDYQSLSNILWLTCPYLNDVIHQLENKGYITKIGNLINSDNQLKQEMAKAH
                     AHFYFLRKSLYEKYFGTDINDDYLNILKRGVGGLQDTIHIKCLHLHYAHYRICESNVA
                     GRIVYHLLQQKVNCDDAICRVSLQK"
     gene            9205..9873
                     /locus_tag="PLJ75_09380"
     CDS             9205..9873
                     /locus_tag="PLJ75_09380"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPD05465.1"
                     /translation="MMQFVESRCKNDDIIIIKKFKKDGSIDSEVINRVSHILLQNGIV
                     VLPVDSQYEIVGMSNSIVEQKIRNLIKSRSKKFVRLITSFKMLESLATVSKLQYDFLH
                     RIWPGEVTVIVPQRQNTDKEIALRYPKTKFVHEIIERTGLPLIAVNIMHITRGSAQQQ
                     DIISNFQKKVDAIVIIEELCKRHPRPTLINIANGKLKIIRVGKVSSEEIQSYFYLGSS
                     DEEI"
     gene            complement(9870..10697)
                     /locus_tag="PLJ75_09385"
     CDS             complement(9870..10697)
                     /locus_tag="PLJ75_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05466.1"
                     /translation="MGNGNSHNELLAYRSYLENNIQYITEINKKIEKLILQLEKEHIS
                     INDCFVHLESYINILLNIYSRFTSPGGYDYCLKLKSLVQKYQQIENSEIQLSALYYIH
                     SKILSLQEEHIATFPSLTPKKDLYRELSPTKNHEEIDVANFAYKWLTFKRNNICFIVL
                     FDSYRIMDISQFNDFKALSQNFYVATIDNSTITIRDPLRLDEQPSRSIVLVTYARDTF
                     GYFYDTIGKKLGAQKNIIARMLTRTQSTSSLQYGRVRLFGDSYIYLKHVDITKDQTL"
     gene            complement(10703..>11575)
                     /locus_tag="PLJ75_09390"
     CDS             complement(10703..>11575)
                     /locus_tag="PLJ75_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPD05467.1"
                     /translation="LKQQLKIGDVVTATINQVIDAGLLVHLDLLDAFIPRSECSYSRV
                     FHPDNFKQGENITAKIIDLDWDKVKITLSIKEILPEPWSFINQFQIGNTYTGTVNKII
                     KNGAFVELKEGLEGFIPLSRMSYSKKINDPHSVCSEGDTVQVTIIDIDESNKKITLEL
                     VTSEENPWLTINNTMTESIYTATIEEITSKGLVVNLPNQIRAFLPLEHCAIDKNADIT
                     KLYQNGNAIKVALLQVDADDRRCLVSEKKAIEVEERSAYQQFAASNSSKGSTAFGQLF
                     KKKYDELLDKGNKP"
BASE COUNT         3679 a         2105 c         2110 g         3681 t
ORIGIN      
        1 tttatttttt caatggttat atagtgaatt aagctattaa ttgccatgga tcgtgcatac
       61 caatcatcat cggttaagct ttggttatat ttcccaaaca tcattttatt ccatttggca
      121 cttttagtac ataccatttc agtaatactc cacaggctat aggtatgttg ccatatatct
      181 gcaagatttg gattactgtc aattaatttt agttggatat aaattataag tatatagtga
      241 accatggggt catattcctt cataaaagat cgtataattt tatcagtgtc taaaaggtaa
      301 tctgctgtaa tctgcctgag aatgctctct ttatttttga aaaaatgata taaacttcct
      361 gttgtaatac ctgttttttt tagtatttga cgtattgtag tctttgaata actctgttgt
      421 acaaacaaag ttcgtgcagc ttctattatt tttgctctag tttgttgatt cttttcaact
      481 cttttcatgg taatattttt aaaacttcat gctaaattat aagtattaca tactataatt
      541 aatgatatat cattaattat agtaaacata aagaaatcca atatataata caagaattat
      601 tttttatgat agttgtccca ttgccggtca ttcaatgagt atagagtagt ataaaaaatc
      661 ccctttgtta taaaggggat tttcaatcat cgatatgcca tactactatt aataattatc
      721 acacagcaac tcaaaatagc tttgaggatg ctgacatgta gggcattttt ccggtgctga
      781 gttaccctca tgaatatatc cgcagttgcg gcatttccat tgtactttct gttctttctt
      841 aaatactttg ccctgttcaa tatttttagc caggtcaaga tagcgctgct cgtggtgttt
      901 ttcaacctca gcaacttttc tgaaaagaaa tgcaatctca ggaaatcctt cttcttcggc
      961 aatctttgct gattcaggat aaagtttcgt gtgctcttcg ttttcaccgg ctgcagcagc
     1021 tttcagattt tccaaagttg tgccaataac tccagcagga taagctgcct gaatctcaac
     1081 catgccacct tcaagataac taaaaaaacg ttttgcatgc tctttttcat tttctgcagt
     1141 ttctaaaaat aatgcggata tctgttcaaa tccttctttt ttagcaactg atgaaaaaaa
     1201 ggtgtagcgt gtgcgtgcct gcgattcgcc tgcaaatgca gctaatagat ttttttcagt
     1261 ttttgttccc tttacacttt ttgccattgt tttattatcc tccgtaatgg tagattatgc
     1321 tttccacaaa ccatgtaaat tgcagtattc cctgactgca aatgatgctg tatcaacctt
     1381 aaaaattgcg acaggttttt cacctggttt taaaaactgt cgatatgact tgcttccagc
     1441 tataagctgt atccattcaa tatagtgctt ttcctccatg ggatgttcaa cactcccaac
     1501 ctgaactttt attccatcag atgttttttc cgcaaccggc acgtgttttt cttttgatgc
     1561 atctacggta ttttcaacct gcaatttcat gggctgtccg cagcatacaa gctcgccttt
     1621 accttcatgc aatacctcaa cgatattgcc acaaatatca catttataca cttctaattg
     1681 ttttgtagcc atttactcct ccttgtaaaa ataatattta acgaaaaaat ataaacctgc
     1741 tttgatactt cattcactat tcattcagta aatgatgata actgcactat catgacactt
     1801 tccctttaaa aaccttatac acataaaaag tataggcaat aactatgggc atacctatca
     1861 acgctatgat ggacattatc ttcaaggtgt acaccgttga agaggcattc acaatcgtaa
     1921 tattataggc acctattgtt gctcgtacca gattgggata aagagcagcg cctgccatta
     1981 tccacagtgc aataaatgcg aaggatgatc ctctcagtgc attcactgct cgtttatgct
     2041 tatttgacaa atatacatag cttaatgcta ctgcataaac aatagcacct atccatccaa
     2101 tactacttga aaggatgtca ggcagaaaaa tgatagtgag aactatcgcc agaataaaaa
     2161 atacggcgtt agcaataagt aatttgttta gaatgctgtg tgcacgtttt gccacttcac
     2221 cttcaatttt atgtattgcc catgtgcatc cctgcagaag tatagccgat agtcctaaca
     2281 aaccaataac aagtggaaaa gggcgcaaca gtgtaaagaa atttccggta aagttcatgt
     2341 tgatatcaat agggattcca catataacat taccaagggc aacaccaaat agtaacgcag
     2401 gtaaaaggct gccagcagta aaagcagttg cccatatttt tctgcgcttt tcatcatagt
     2461 accagaattc cattgaaaca gctctaaata tgagagcaaa cagcaccagc atgagtgcta
     2521 aataaaaacc actgaaaacg gttgcataag caaatggaaa agcagcaaat aatgatgcac
     2581 cagctgtaat gagccatact tcattaccat cccatacagg ccatatagag tccataacct
     2641 ttttttgttc attttcgtct ttagctaata caggcataag actggcaatg cctaaatcaa
     2701 atccgtcaag aattgaataa accattaaca aaaaacctat aatgataaac catatattct
     2761 gaaatatatt tacatccata ttcaaacctc catactcatt agtatatttc tgattgcggt
     2821 ccctttttga taattttagt cagtatcata ataaacatca ctataagcat cagatagatt
     2881 aatgataata taaccaatga aaaagctaca tgcgaagcgg gaacaattat tgatactgca
     2941 tctgctgttt ttaatacttt ataaactgcc catggttggc ggccaacctc agcagcaatc
     3001 catccaaact cattggcaat gattggtaaa gggattgcaa aaaacaacac ataccagaac
     3061 catcttgcat tgaatagttt ttttgtaagc agcaacacaa tacttattat ccctataagc
     3121 gcaaacagtg aacccaatcc aatcataata tggtatgaaa agtaggggag caccacaggt
     3181 gggagttcat catcagggaa cgcatccctt cctttaactt cagcgttggg atcaaaatag
     3241 gcaagtaaac taagaagttt gggaatttta atttcataat atgttttctt ctggctttcc
     3301 acagggatgc caattattga cattggtgcg ccctgttgtg tttgccacag cgcttcaaag
     3361 gttgccatct tttcgggctg tattctggtt acctgtactg aatggatatg gcctgatgca
     3421 aactgcgcca atgacatgat gcagaatata atcaatgcag ttttgaggat atgttgagct
     3481 tcatttgtat gtaatcgctt tattaaatac catgcagcaa tgccagcagc aaaaagcgaa
     3541 ccggtaatcc agccggctat gattacatgg gcaaaccgca ccagcgtgga agggttaagt
     3601 gcagcagccc agaagttggt taaaactgct ctaccacctt caatggcaaa gccagcaggg
     3661 gtttgcatcc atgaattggc aatgataatc cataaacctg aaagatgcga gccaaagaat
     3721 accagaaacg ctgaaagcaa ataaaccttt tttgataccc tgttgcgggc aaaaagcagc
     3781 acccccagaa atactgattc aaggaaaaaa gcaaatatcc cttctgctgc aagtggcgca
     3841 ccaaatatat cgccaaccat tcgggaatat tcagcccagt ttgtaccaaa tgaaaactca
     3901 agcacaatac cggttgccac acctaatgca aacacaagcc ccagtatttt aataaaaaat
     3961 gaactaatag ttttaaaaat gtcatcatct gttttgtaaa acttatattc atagataagc
     4021 actaaaaggg taagccccag tgttaatgaa ggaaatataa agtgaacacc agcggccagt
     4081 ccaaattgta tacgtgaaag cattacaaca tccatacata aacctccaga gaaatatttt
     4141 tataaatata acggatatgc ttacctgagc atatcccgac agtttacagt attttaatcc
     4201 agcggttcaa attgctcttt ggtggcacca caaatagggc aaacccaatc atccggcaaa
     4261 tctttgaaag cagttccagc ttgtatgccg ttgtcgggat cgccttcttc aggatcataa
     4321 atataaccgc aaaccgtgca ttcgtacttt ttacccatat aaataattcc tccttatatt
     4381 gtattgtaaa ttgtaagatg aaaaatgcaa aatctaaagt gttaaatgca aaaatgtaaa
     4441 gtaaataaat tgctcatcgc cattcaaaac acaatcaatt tattgtgcaa caggtgtata
     4501 ccccgcatcc ttaattgctt tgataatttg attgatatca agagaactat ctactgttac
     4561 tgtttttgag tcaagattgc attgtacttt tgaagatttg tcaatagcct gtatggactg
     4621 ggtaatgtgc ataatgcaat gattacacat catgtcatgt accgtaaatg tatacataaa
     4681 aagctcctat aaacaataag agtattgggc aacactattt tacatcatat cacacatcca
     4741 atcaagtgaa gcacaacaat aaaccattgt taatgctgtg ttctcaatga cagtggcatc
     4801 acaatgtttt ggtaaagctt tacagcatat gtcactcatc actgcgatga aatgtaaaaa
     4861 atcgatacat ttgccagtaa ctatcgccac aaaaatactt taaattataa ttatcacata
     4921 gttataataa ttacaaatat ttttttagta aagcatattt ttttgttgcc ataactcctt
     4981 aatctgccag acagtttttg caaataccac gtaaggctat ttgataacct tcaattgtgt
     5041 gcccttcaat cgtttgtgga aactttacat atggtgtgct atcaatatca aaaacgttac
     5101 cgcattgtga acaggtcaga tgtgcgtggg gcataacagt aatgtcatac cgtgcttcta
     5161 cattatcaag cagcagcatc tgtacaagcc cctgttgaac aaaaaggctg acggtattat
     5221 aaaccgttgt tttagacagc gtagggatga gcggaagcag tgcggaatag atttcatcaa
     5281 cggttgggtg cgacctgttc tggtctaaat attccagtat ctttaatcgc tggtacgatg
     5341 gcgagattcc ttttgattct aaatagtgtt tgtattgttc catagtggtt accgttataa
     5401 tggtattatg aactcattgc atgcttttat aaaatacaaa gttttatgtt aaaagtcaat
     5461 ttcattaatt ttttcataaa cctcctgggc gatagttacc agcaattgtt tttgtggcac
     5521 ataattaact ttaatagcgg gaagcaatcg ccagcccatc tgctgcagta cgtcctctac
     5581 ctgcgcaacg ctctggccgc cccaaccata cgacccaaat gccagtccca cacggttctt
     5641 tggtgccaga cctttgcagt aggttaagaa tgcagcaacg gttggcagca tgttattgtt
     5701 aagcgttggt gatccaacac agatatagtg tgcgtcaagt acttcggtca taatgtctga
     5761 aatatgtata gttttcaaat cataaagctg tacgggaata tttttttcct caaaggcatt
     5821 ttttatagcg tgggcaatta attttgtgga attccacatg gtgtcgtaga tgatgagagc
     5881 tttgttggtt gttttattgg cagcccagct cctatacatg gttactatcg cctgaacatg
     5941 atctttccat agtatgccgt gacttggtgc aatcatggca atgtctaacg tacttacggc
     6001 atcaaggact ttctgtgttt gcttgtcata gggcaaaaca atattggcat agtatttttt
     6061 tgcttcattc atgataatgt caaggggata ttctgtatca aagcgttctg atgttgcaat
     6121 atgctgtcca aaggcatcgt tggaaaacaa aattttagtc tcaggcatat aacaggccat
     6181 gttgtcaggc cagtgaacca tctgctcaag gataaattgc aagcttgttt ttcctaatga
     6241 tatgctatcg ccggtcttca tgacggtata gttccagttt tttttgtagt gagcttccaa
     6301 tccctttgca ccgtttggcg aggttataag catggcattg gggcacaatt ccataagctg
     6361 tggaagtccg cctgaatgat ccatttcaac atggtttgat atgataatat caatcttaga
     6421 agggtcaaca atgcatgaaa tgcgttcaag catctcatcc atgagataat atttaacggt
     6481 atcaataagg gtaatctttt catccatgat taaataggca ttgtatgttg aaccacgctg
     6541 tgtaaggtaa ccgtgaaagt ttcgcaaatc ccagtcgata ccgccaaccc agtatataaa
     6601 gtcattcagt gcaattggtt tcattgtacg ctccttttta ttatactatg gtaattattg
     6661 caatagttaa attgatagca actgtgctga attgttcaag aattattttg tatgaaatat
     6721 atttttcagt gatgcaaccc ccctggccct ccctttatca aagggatggt aacgttgtaa
     6781 attggctatc cgttagtaaa gggagggtta aaaataacta tcgaacatat attcccatta
     6841 tactataaac caaaaactaa aattatactt gcacattaca gctatcaatg attgcattga
     6901 ttatgaaaat gtattactta tagataatgt atattataat tatgaaggag ttgtatcatg
     6961 actattataa ccgatgtcca tgcacgtgag attttggatt cacggggcaa tcctactgta
     7021 gaagttgatg ttgagctttc ttcagggatt gctggcaggt ttgctgtgcc gtcaggtgca
     7081 tcaactggcg aacatgaggc aattgaatta agggatggcg acaaagcacg ttataatggc
     7141 aagggtgtgc ttaaggcagt acaaaatgtc aatgaaatta tatcaggcgc tgtcgttgag
     7201 atggatgcat tacgtcagat tgagattgat aaaacgttaa tagatcttga tggcacacca
     7261 aacaaagcta aattaggggc taatgcaatt ttaggtgtat caatggcatg tgccaaagcg
     7321 gttgctgaaa cattggggat gccattatat caatacattg gcggagttgg agcatgcgag
     7381 ttgcctgtcc caatgatgaa tatcctgaat ggtggaaaac acgctgataa taatgttgat
     7441 attcaggagt ttatgatagt tccggtaggt accgatagtt tcaaagaagc attgcgcatg
     7501 ggtgttgaaa cattccacgc tttgaaaaaa gtattaacca aaaaaggata taataccggt
     7561 gtaggtgatg aaggaggttt tgcaccaaat ttaaaatcca atgccgaagc ttttgaggta
     7621 attctggaag caataaaaga agcaggcttt aaagccgggc aggacattta cctggctata
     7681 gatgctgcag caagtgaatt ttataaaaac ggtaaatatg tgatggcagc cgaagaaaaa
     7741 ccagaaaaaa catctgagga gatggtcaat gtttatcttg aatggataaa taaatatcca
     7801 attatttcaa tagaagatgg tatggccgaa aatgattggg atggctggaa gctgttaact
     7861 gataaagcaa agaatacggt acagcttgtt ggtgatgatg tatttgttac caacacatca
     7921 atcttccaga agggtattga aaaaaaggta gccaattcca tacttataaa attaaatcag
     7981 attggtacgt taaccgaaac tatcgaagca attgaaatgg caaagcgtgc tggttacact
     8041 gctgttatat cgcatcgttc aggtgaaact gaagatcctg ctattgctga tttagcagtt
     8101 gcaatgaata ctggattaat taaaacaggt tcggcttccc gtactgaccg tttagcaaaa
     8161 tataatcagc ttcttcgtat tgaggagcag cttggtgttt ttgcaaaatt taatggcaga
     8221 agggtatttt attccattgg taaaccataa cacaaaattt acattgttta ttgttatact
     8281 tatcctcttc agtgtgtact tctttgtatt gagcgatagt ggcatactga agagagtaat
     8341 tcttaaacat gatatccagc atatacagga tcagatagct ttgaaacagc agcagattaa
     8401 taagatcaat cggctggaca ataataaaat gtattcaatg tacatgttac aggaaagcat
     8461 caatgctggt tatatcccca aaggagcaaa ggtttatcag ttcaaggata taccagatag
     8521 tacaaaacat agagaagact cattatttca aacacaacat gagaaatatt tgcaatatat
     8581 ccagtatgga cgaatattct ggattacatt ttctgctttg ataatgttag gagtgttgct
     8641 ccatcataga agaaaacctg tagtgctata atgaatacta tatgatacca tacaattcta
     8701 aacttaatca gcgagatttg caggttttac aatggcagct taaaggaacc actgatgctg
     8761 tttccggtat atttgaaaaa tgcaactttg gatttcctca aattattctt ttatcaccta
     8821 tagttaatga tttaatagat tatcaatcat tgtccaatat tctgtggctt acatgcccct
     8881 acctcaatga tgtcatccac cagcttgaaa ataaaggata cataacaaaa attggaaatt
     8941 taatcaattc agataatcag ttaaaacagg aaatggcaaa agcacatgct catttctact
     9001 ttttaagaaa gagtttatac gaaaaatatt ttggtactga tattaatgat gactatttaa
     9061 atatattgaa aagaggcgtt ggtggattac aggatacaat acatataaaa tgcttgcatt
     9121 tacattatgc tcattataga atttgtgaaa gtaatgttgc gggaaggatt gtgtatcatt
     9181 tattacagca aaaggtaaat tgcgatgatg caatttgtag agtctcgctg caaaaatgac
     9241 gatatcatta tcataaaaaa atttaaaaaa gatggtagta tcgatagtga agttataaac
     9301 agggtatctc atattttact tcaaaatggt attgttgttc ttcctgttga ttctcaatat
     9361 gagattgttg gtatgtccaa cagtattgtt gaacaaaaaa tacgaaattt aataaaatcc
     9421 agatcaaaaa aatttgtacg actcattaca tcatttaaaa tgttagaatc gcttgcaacg
     9481 gtaagtaagt tgcaatacga ttttcttcac aggatatggc caggggaagt aacagttatt
     9541 gttcctcaac ggcaaaatac agacaaagaa atagcattgc ggtatcctaa aacaaagttt
     9601 gtacatgaga taattgaacg cacagggctt ccactcattg ctgttaatat tatgcacatt
     9661 accagaggtt cagcccagca gcaggatata atcagcaatt ttcagaaaaa ggttgatgct
     9721 attgtaatta ttgaagagtt atgtaaacgt catcctcgtc caactctcat caatattgca
     9781 aatggaaaac ttaaaataat aagagttggc aaggtgtcat ctgaagaaat acagtcatat
     9841 ttttatctgg gaagcagtga tgaagaaatt taaagtgtct ggtcttttgt tatgtcaaca
     9901 tgtttcaaat aaatataact atcgccaaaa aggcgtaccc ttccatattg tagcgacgat
     9961 gtgctttgtg tccttgttaa cattcgtgca ataatatttt tttgtgcacc cagctttttc
    10021 ccgatagtat cataaaaata accaaaggta tctcttgcat aggtaacaag cacaattgag
    10081 cgcgaaggct gttcatcaag cctcaggggg tcacgtatgg taatggtgct gttatcaatt
    10141 gtagcaacat aaaaattttg tgaaagcgcc ttaaaatcat tgaactgtga gatatccata
    10201 atacggtaac tatcaaataa aacaataaag cagatgttgt tgcgtttaaa cgttaaccat
    10261 ttgtaagcaa agtttgccac atcaatttcc tcatggtttt ttgtgggcga tagttccctg
    10321 tataggtctt tttttggcgt aagtgaaggg aatgttgcga tatgctcttc ctgcaatgac
    10381 agtatttttg agtgaatgta atagagcgca gaaagttgaa tctcagaatt ttcgatctgc
    10441 tgatatttct gtacaagtga ttttagcttt aaacaatagt catatcctcc aggcgacgta
    10501 aaacgcgagt agatgtttaa taatatatta atataacttt caagatgtac aaaacagtcg
    10561 tttattgaaa tatgttcttt ttcaagttgc aaaataagtt tttcaatttt tttatttatc
    10621 tcggttatat attgaatatt attttcaaga tatgacctgt aggctaaaag ttcattgtgt
    10681 gaattgccat ttcccatgat aattatggtt tattaccttt atcaagcagt tcatcatatt
    10741 tctttttgaa aagctggcca aatgcggttg atcctttgct ggaatttgaa gcagcaaatt
    10801 gctggtatgc actgcgttct tcaacttcaa ttgctttttt ctctgatact aaacatcgcc
    10861 tatcatcagc atccacctgt agtaaagcaa ctttaatggc attgccattc tggtacaatt
    10921 ttgtaatgtc tgcatttttg tcgatagcac agtgctctag tggtaaaaat gctcgtatct
    10981 gatttggcaa attcactaca agtccttttg atgtaatctc ttcaatcgtt gcagtatata
    11041 ttgattcggt catagtatta ttaatagtaa gccacggatt ttcttcactg gttaccagtt
    11101 caagtgttat ctttttgttg ctctcgtcaa tatcaataat ggtaacctgg acagtatcac
    11161 cttcgctgca aacactgtgt ggatcattaa tttttttact atagctcata cgggataggg
    11221 gtataaaacc ttcaagccct tctttcagct caacaaatgc tccattttta attattttgt
    11281 tgacagttcc tgtataggta ttgcctatct ggaattggtt aataaagctc catggctctg
    11341 gtaaaatttc ctttatactc aaggtaattt ttactttatc ccaatctaaa tcaataatct
    11401 ttgctgtaat gttttcaccc tgtttaaaat tatcagggtg aaatacccga ctatatgaac
    11461 attcacttcg aggaataaaa gcatcaagta agtccagatg taccaacagc cctgcatcta
    11521 ttacctgatt gatggtagca gtaacaacat caccaatttt gagttgttgt ttaag
//
LOCUS       DAOOJH010000074        11521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5130_length_11521_cov_8.707047, whole genome shotgun sequence.
ACCESSION   DAOOJH010000074 DAOOJH010000000
VERSION     DAOOJH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5130_length_11521_cov_8.707047"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLJ75_09395"
     CDS             complement(<1..502)
                     /locus_tag="PLJ75_09395"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPD05468.1"
                     /translation="MPIDTALTSVLIHSGMMQFLKDGQPAVNLRNRLQKPCPLVLDGA
                     MGTMLFDALPDYNGSLELLNMERPDVIISIHKQYIDAGADIIETNTFGGNALKLQQAN
                     LDSRCEEINKAAAVIAKQAASNRAFVAGSVGPTGTLVEPMGTTPDEAVYNAFKKQIKG
                     LVDGGVD"
     gene            complement(645..1385)
                     /locus_tag="PLJ75_09400"
     CDS             complement(645..1385)
                     /locus_tag="PLJ75_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPD05469.1"
                     /translation="MKKLYKALIETIYFIYRPVFYVLVVIDTTILGILTIALSFFDPT
                     GNTVHYIGVFWSQLNLFLSGVKVRVIGKENITKNQPYIVMMNHQSYYDVWAVIGYIPL
                     QLRWVMKMELRKVPIFGLGCERMGHIYIDRGDSQQAHESLKVAVEKIRNGASVVFFPE
                     GTRSIDGNMLPFKKGGFVIAIESKVPILPITGIGSRSILPKGSLAIRPGTIHLYIHPP
                     IEVTAYSQQNKEALMQRVRDVIQSKLAS"
     gene            complement(1626..3116)
                     /locus_tag="PLJ75_09405"
     CDS             complement(1626..3116)
                     /locus_tag="PLJ75_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPD05470.1"
                     /translation="MAFNEYEKYDATGLAELIQKKKVSVYEVINEAIQRMQTINPVIN
                     AVITPMYENIDARIKELPKGRFYGVPFFIKDLLTTYKGVRMASGCRAYKNYIPNFHSE
                     VAKRYINAGVVILGKTNTPEFGLMAYTEPEAFGPTLNPWNIALTPGGSSGGSAAAVAS
                     GMAPFAGGGDGGGSIRIPSAYCGLFGLKPSRGRVPAGPEYGQIWQGAAVEHVLTRSVR
                     DSAAMLDILQGADIGAPYIIEPPEMPYTKLMQKSPGKLTIAISTESPIGTQVHPECKK
                     AAQEAAKLCERLGHHVEYDTPDIDGIALAKSYLMLYFGEVAADIATIGLTLGRKTKQS
                     DVEITTWTLGMLGKAYGAGDFVLALREWNKASRILGQFFTKYDIYLTPTTAQPPAPIG
                     SLKPKPIELFASKIMNTLGLGKLLKASGIVDKLAVESLSQVPFTQLANLTGVPAMSVP
                     LHWGSDGLPYGVQFIASSGKEGLLLQLAAQLEKAKPWFNKRPDLTI"
     gene            3498..4016
                     /gene="def"
                     /locus_tag="PLJ75_09410"
     CDS             3498..4016
                     /gene="def"
                     /locus_tag="PLJ75_09410"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPD05471.1"
                     /translation="MITDTQHTLVYYGNNILRTTAQEVVQISQMVRDLVDEMFAIMHR
                     EQGIGLAAVQIGVPLQVIVIDIRSNKGPMMALINPEITAFSEEKDVYEEGCLSLPGVM
                     ADVIRPLSIQVEGIAINEKPVKFEASGLLARVLQHEIDHCNGKLFIDYLEDYKRKELN
                     SQLKKIKKLNRE"
     gene            4016..4948
                     /gene="fmt"
                     /locus_tag="PLJ75_09415"
     CDS             4016..4948
                     /gene="fmt"
                     /locus_tag="PLJ75_09415"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPD05472.1"
                     /translation="MKIGFFGTPDIASYCLDYLLKKYDIAFVITTPDKPKGRSKDPVP
                     SPVKEKAAALGIPCFQPLNLNDTAIIQTLKNFHCDIFVVVAYGKLIPGDVFLLPPLGT
                     INLHPSLLPKYRGAAPVQWALYNGEHEIGITVQLINEQLDAGDIVMQKTIPVDLHDTS
                     ADVYERIKPIGAEMLAESVDCLFKGTIAPKPQDHALATYCGKITTETAHINWELSSVQ
                     IHNMVRAFNPKPIAWTMFREKLIKIWKTDLPDNSSEKMIGQPGTLVPASKRLFVCTGR
                     GMIEVLLLQPETKKPMDAIAFLNGYRLQEGERFI"
     gene            4952..5299
                     /locus_tag="PLJ75_09420"
     CDS             4952..5299
                     /locus_tag="PLJ75_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007280916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPD05473.1"
                     /translation="MGDRYRKFFDDNATVSGINFSDNELHIKREKQKARELRHSSWWK
                     KKIARGVCYYCGRKFKPSELTMDHVVPLSRGGHSEKINIVACCKECNTKKRSCLPVEW
                     GEYIENIKKSGIT"
     gene            5405..7834
                     /locus_tag="PLJ75_09425"
     CDS             5405..7834
                     /locus_tag="PLJ75_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD05474.1"
                     /translation="MQSSDTEKIRKGEILIVQGEEPRFIYYLQSGSVEILSAPSEYEG
                     LDSTIIVDKSKRVGIINEKTVIAGLSLLFTEPYKKTIRAIEDCIVKKYPLRDGGFRQI
                     VMDDPSFAAVLLTHVIKRFEVSLTDVKRYTLLYQNLCRINDNFSLIAGELADNLTDTL
                     SQRADDIYKTYTQNGGDFPSRIDAKFIVQDNSNLLKKKYTFPGLPVENLVDIKQCNFL
                     KKIVTMDNQLLITMFRNDPTIPVYMFNCISENLVKLLDRIESIHNEIDVEMDYLFGKD
                     GFIAYLIDKQKFKLWVASGRVSDGFLKGFMSIANKIYQFYQEISGENLSKKFDSFKKL
                     HNYIQITQDQPKVAEEVQRVSRISQNISQLYKNSVYQIFEFGFIEKENQKNLLKLLSD
                     FKKMKDPLSSEIDARKVRRQIARFYHDIFAKVFVRSQTESVVPPPVKLMLRFGFFDET
                     MIEQEQLNELHEISMRRVEQTQTPIVYEDEFLTRIYNGDENPSINEMGLSYEAHLREE
                     QKHIKGSSNIDATDNVKKIIYEINHRIATATSVCSGSTATSFPILTSLNMKGSISHYY
                     VSKKKLESIVSALRDVDFSVFYRETAVMLGNTKELIKEEVVPYFILLPSFGSKTMLWQ
                     ELDGTNRRTRGRIVVPILFMGDMMPTMAHTFATFRWELNRTIMGGMWADPIEGGLTGA
                     YFDYINTFKKNSKLSLEAKQKVVERFKSIRNNRDRFADDYMQWVLYEKDGIMKLNNVV
                     RDMFYRHIPFKKELRDRLENMPAYTEIAGRFRNVFNREVGNYERKFKKYQREDGTLPE
                     ALQKFMEFLYK"
     gene            7840..8649
                     /locus_tag="PLJ75_09430"
     CDS             7840..8649
                     /locus_tag="PLJ75_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05475.1"
                     /translation="MNSHRITGIIRHYTRKARDQIYNAGLIIFKEAGIVEHLDDISAC
                     VDELIKNAVKANYKHLLIIQALQRQFQLQYKDKSQSQIHEYISDILKDKIQFDTLASI
                     VLKKENIAQTVREVLNQEGIFLSIKNRAYQENRTLRKDELDAINTLQLFLDLQDTMKK
                     KNIQILLKVDYRDDYIYIEITNTAPILSHDLQRIYDKREEYRKYRNEGREYEFFINNI
                     DTSDSGFGLGYAKIDSYLYNLGIEPDKAVTVISINNTTAMLSIPVEALKKL"
     gene            9049..10671
                     /locus_tag="PLJ75_09435"
     CDS             9049..10671
                     /locus_tag="PLJ75_09435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPD05476.1"
                     /translation="MPTLQFKGKNIIWNHHLSIPYHTLDEVDELHYKPEKANGNIIIE
                     GDNLLALKALLPQYSGRIKCIYIDPPYNTGEENWVYNDNVNSPLIKEWLGKEVGTDCL
                     VRHDKWLCMMVPRLKLLKELLTKDGIIFISLNDIENHYCKVICDEIFTKENFVANIIW
                     EKRTARDARRAFSINHDHILVYANDYESFYNSRNLLPVTDEVLSRYSNPDNDPRGPWQ
                     SVAMTAQAGHATKDQFYTVITPTGKKHNPPKGLCWIFTEKKFKEEVKNNNIYFPKNGD
                     GVPRQKKFLEERGDTGLVPLTIWTSKEVGTNDSAKKSLLEIFHDELHFDNPKPVELLR
                     RIINISTSSSDIILDSFAGSGTTMHAVMDLNKEDGGNRQCILVQMTEATEAEPEKNIC
                     KDITRERIKRAIDKYGYDSGFKYFRVGIPIDAESLLSGKLPTFKQFAEYVYYLCTGEH
                     IKDKSVINEKTYLVGSINNSVIYLVYKQNFDELTRLALNLPLAEKIIADNPRKKIIVY
                     APACFLEEDYMKENNIEFVGIPYNLFRRENGA"
     gene            10675..>11521
                     /locus_tag="PLJ75_09440"
     CDS             10675..>11521
                     /locus_tag="PLJ75_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD05477.1"
                     /translation="MFLKKYQQKVVTELKFFYSKARETKDAFDTARKSLPENMRHTLN
                     WVQTTFQNTNKEYKDRCTNGLSEYYPRIVIKVPTGGGKTLLAVEAIREYQNIFARKRT
                     GLVVWIVPSETIYSQTVQKIRDKSNPLRQLLDQASGNKTLVIEKGQRLTIHDIEEHLV
                     ILFVMIQSISRKNGKEALKVFQDSGGFESFFPADNRNDLHSELLKSFPNLDYIAPIGS
                     MFPLIKTSLGNAVRVSRPFIIIDEIHKVFSDTARKTIDNLNPEMVLGLTATPRVEMNI
                     LVSITG"
BASE COUNT         3820 a         1988 c         2257 g         3456 t
ORIGIN      
        1 aatccactcc gccatctacc aaccctttga tctgcttctt aaatgcatta tagacggctt
       61 catcaggggt ggtgcccatt ggttccacca gggtgccggt aggcccaacc gagccggcaa
      121 caaacgccct gttgcttgcg gcctgttttg caatgacggc agcagcctta tttatctctt
      181 cacaacggct atccagattt gcctgttgca gcttcaaagc attaccgcca aatgtattgg
      241 tttcaataat gtcagctcca gcatcaatgt attgcttgtg gattgaaata ataacatcag
      301 gacgctccat attgagcaat tcaaggctgc cgttgtaatc aggcaaagca tcaaataaca
      361 tggtccccat tgctccgtcc agcacaaggg ggcagggttt ttgcagtcgg tttcttagat
      421 tcaccgcagg ttgaccatcc tttaaaaatt gcatcatacc actgtgtatc aatacagagg
      481 ttaaagccgt gtctatgggc ataaaaagct gttatcagga actatcgcac aagaaaaaat
      541 agaatggaaa aatatacaat aaaaaaattc acattttatg attacgacac aataatacgt
      601 tccggtgtca atagtattta tgattattta ttgtttatgc aatctcatga tgccaatttg
      661 ctctgaataa catccctgac cctttgcatc agagcttcct tgttttgctg gctataggca
      721 gttacctcaa tggggggatg tatatacagg tggatggtgc cggggcgtat tgctaaactc
      781 ccctttggca gtatggaacg cgaaccaata ccggttatgg gtaaaatggg cacctttgat
      841 tcaatagcta tcacaaaacc acccttttta aagggcaaca tgttcccgtc aatgctgcgc
      901 gtgccttccg ggaaaaaaac tactgaagca ccattgcgaa ttttttcaac ggcaacctta
      961 aggctttcat gtgcctgttg tgaatcacca cgatcaatat agatatgccc catgcgctca
     1021 cagccaagac caaagatagg aaccttgcgc aattccatct tcatgaccca gcgtaactgt
     1081 aaagggatgt agccaataac agcccagaca tcatagtaac tttgatgatt catcataaca
     1141 atgtaaggct gattctttgt tatgttttct ttgcctatca cccttacttt gacaccggaa
     1201 agaaatagat ttaactgtga ccagaataca cctatgtaat gaacggtgtt gccagtagga
     1261 tcaaagaaag aaagtgcaat ggtcaaaata ccaaggatgg ttgtatcaat aacaaccaga
     1321 acataaaaaa ctggcctgta tataaaatag attgtttcaa tcaatgcttt atataatttt
     1381 ttcatgaata cctccatcct ggaggcacat tgaaaaccac cttttacatt tgatcaacac
     1441 tattgtcagt gtaacagcga tcaacaaagt ggttttacca ttttttgtgc ccagcataat
     1501 aaaaaattta gttttcaata tatacatact atctgataca ccttttaaaa aacttgagga
     1561 atatcagcag ttacatacag ctgtatcaac aactttatac tataacaatt tactcattat
     1621 attagtcata tagtcaaatc tggtcgcttg ttaaaccatg gttttgcttt ttccagttga
     1681 gctgcaagct gtaatagcaa tccttcctta ccagatgaag caataaactg aacaccatac
     1741 ggtaaaccat cactacccca gtgcagggga acggacattg cagggacacc ggttaaatta
     1801 gctaattggg taaacggcac ctgtgataaa ctttctactg ctaatttgtc aacaatcccg
     1861 gatgctttca gcaattttcc caaacccaat gtattcatga tctttgatgc aaaaagctca
     1921 ataggttttg gtttcagtga accaatgggc gctggcggct gagctgttgt tggagtaagg
     1981 tatatatcgt atttagtaaa aaactgtccc agtatgcgtg aggctttatt ccattctcgc
     2041 aatgcaagaa caaaatctcc agcaccataa gcctttccca gcatccctaa tgtccatgtt
     2101 gttatctcaa catcgctttg ttttgttttt cttcccaacg ttagcccaat ggttgcaata
     2161 tcagcagcaa cctcaccaaa ataaagcatc agatagcttt tggcaagcgc tataccgtca
     2221 atatctggtg tatcatattc aacatgatgt cctaaccgct cacacagttt tgcagcttcc
     2281 tgtgctgcct ttttgcattc aggatgtacc tgggtaccaa tgggcgattc agtagagatg
     2341 gcaatggtaa gttttcctgg tgacttttgc attaatttag tgtaaggcat ctctggtggt
     2401 tcaatgatgt atggtgcgcc aatatcagca ccctgcagta tatccagcat ggctgcgcta
     2461 tctcgcactg agcgggttaa aacatgttct accgctgcac cctgccatat ttgcccatat
     2521 tccggaccgg caggaactcg cccgcgcgat ggtttaagcc caaataatcc acaatatgct
     2581 gacggtatcc gtatagaacc tcctccatca ccgccacctg caaaaggagc catgcccgaa
     2641 gcaacagcgg ctgcagaacc accgctggaa ccgccagggg tcagagcaat gttccatggg
     2701 ttaagggttg gaccaaatgc ctctggttcc gtatatgcca ttaatccaaa ttctggagta
     2761 ttggttttgc ctaatattac aacacctgca ttgatgtacc gtttggcaac ctcactgtga
     2821 aaattgggga tataattttt atatgccctg cagccgcttg ccatcctcac gcctttatag
     2881 gttgttaata aatccttgat gaaaaatgga acgccataaa aacgcccttt tggcagctct
     2941 tttatccttg catcaatatt ttcatacatg ggagtaataa cagcattgat aacaggattt
     3001 attgtttgca tacgctgtat tgcttcgttg atgacctcat ataccgatac tttctttttt
     3061 tgtataagct cagcaagccc ggttgcatca tatttttcgt attcgttaaa agccatggta
     3121 ttcctccaga aataaaatat agtgtgtcat tcagaataat atatcagagc aacaacaaca
     3181 taagttgctc aactacatcc agtacaggtg ctatcgttca taatgatagc agtgttgcat
     3241 tttatcctgt tttaagtaat tgcatggtaa ctatcgtttt agttgtattc tgcacccaga
     3301 aattatttgg taacacagga taaactgctg gactaaaatc gtatcaaagt gtatgaaaaa
     3361 tataacaaat tgtcaattag tattgtgata aattacagta aaaataagtt ttcgaaaatt
     3421 catatcttga catgctatgc tctgtgtgat atattaatta taattaatta tatataaaat
     3481 gatattgtga gatagttatg ataaccgata cacagcatac attagtctat tatggcaata
     3541 acatattgcg gacaacagcg caggaggttg tccagatatc acaaatggta cgtgatttag
     3601 ttgatgagat gtttgccatt atgcatagag agcagggtat tgggcttgct gctgtgcaga
     3661 ttggtgtgcc attgcaggtc attgtcattg atatacgctc taataaagga cccatgatgg
     3721 cactgataaa tcctgagatt acagcatttt ctgaagaaaa agatgtctat gaggagggtt
     3781 gtttatcgtt gccaggagta atggcagacg ttattcgtcc gctatctatt caggttgaag
     3841 gaatagctat taatgaaaag ccggtaaagt ttgaagcatc agggttactt gcacgggttt
     3901 tacagcatga gattgatcat tgtaatggaa aactatttat tgactatctg gaagattaca
     3961 agcggaaaga attaaattcg cagcttaaaa agataaaaaa gcttaacagg gaataatgaa
     4021 aataggtttt tttggtacac cggacattgc ttcatattgt ctggattatt tacttaaaaa
     4081 gtatgatatt gcttttgtga tcaccacccc tgataaacca aaagggcgca gtaaagatcc
     4141 tgtcccttcc ccggtaaagg aaaaggctgc tgcattgggc attccgtgtt ttcaaccgtt
     4201 gaatttaaat gatactgcaa taatacaaac actaaaaaat tttcattgtg acatctttgt
     4261 tgttgttgcg tatggcaaac ttattcccgg agatgttttt ttgttgcctc cattaggcac
     4321 tatcaatctt catccatcgc ttctgccaaa atatcgaggt gctgctcctg tgcagtgggc
     4381 attgtataat ggtgaacatg aaattggtat tactgtacaa ttaattaatg aacagcttga
     4441 cgcaggtgat atcgtgatgc aaaaaactat ccctgttgat ttgcatgata cctcagcaga
     4501 tgtttatgaa aggattaaac caattggtgc agagatgctt gcagaatctg ttgattgtct
     4561 ttttaaagga actatcgccc caaaaccaca ggaccatgcg cttgctacct attgtggtaa
     4621 aattacaact gaaacagctc atattaactg ggaactgagt tcggtacaaa tccataatat
     4681 ggtcagggca tttaatccaa aacccatagc gtggacgatg ttcagggaga agcttataaa
     4741 aatatggaaa acagatttac ctgacaattc atcggaaaaa atgattgggc aaccggggac
     4801 attagtacct gcttcaaaac gcctgtttgt ttgtaccggc aggggtatga ttgaggtgct
     4861 tttactgcaa cctgaaacaa agaagcctat ggatgcaatt gcatttttaa atggttatcg
     4921 attacaggaa ggggaacggt ttatataata tatgggagat agatacagga aattttttga
     4981 tgacaatgcc actgtatctg gtataaattt ttctgataat gagttgcata ttaagcgtga
     5041 gaagcaaaaa gcaagggagt tgcgacattc ctcatggtgg aagaaaaaaa ttgccagagg
     5101 tgtttgttat tattgtggca gaaagtttaa gccatctgag cttaccatgg accatgtggt
     5161 gcctctctca agaggtgggc actccgaaaa aattaatatt gttgcgtgtt gtaaagaatg
     5221 taatacaaaa aaaagatcat gtttgccagt tgaatggggt gaatacatcg aaaatataaa
     5281 aaaaagtggg attacataaa aattgttatt gctcttttat tggcattgtt cataaatata
     5341 atatgaatgt ttgtttttta tagtaagaaa tataatatca tctctataat ggcaaaattg
     5401 atctatgcaa agtagtgaca ctgaaaaaat tagaaagggt gaaatcttaa ttgtacaggg
     5461 tgaagaaccc cggtttatct attatttaca aagtggttct gtagaaatat tatctgcgcc
     5521 atctgaatat gaaggtctgg atagtactat tattgtcgat aagagcaagc gtgttggcat
     5581 aataaatgaa aaaacggtaa tagccggatt aagtttactt tttacagagc catataaaaa
     5641 aaccattcgt gctattgaag attgtattgt caaaaaatat cctttgcgag acggtggttt
     5701 tagacagata gtaatggacg acccttcttt tgctgctgta ttgttaacac atgttattaa
     5761 acggtttgaa gtatcattaa cggatgtaaa acggtatacg ttattatatc aaaacctatg
     5821 tcgtattaac gataattttt cccttattgc gggggaactt gccgacaacc tgacggatac
     5881 actgtcgcaa cgtgctgacg atatttataa aacatataca cagaatggcg gtgacttccc
     5941 ttcgaggatt gatgcaaaat ttattgttca ggataattcc aatttattaa agaagaaata
     6001 tacatttcct ggactgcctg ttgaaaatct tgttgatata aaacaatgta acttcttaaa
     6061 aaagattgtt acaatggaca atcaattgtt gataacaatg tttcggaatg atcccactat
     6121 cccggtatat atgtttaact gtatttctga aaatcttgta aagcttttag acaggattga
     6181 atcaatacat aatgaaattg atgttgagat ggactatcta tttgggaaag atggatttat
     6241 tgcatactta attgataagc aaaaatttaa gctatgggtt gcttccggaa gagtaagtga
     6301 tggcttcttg aaaggtttta tgtcaattgc taacaaaatt taccagtttt atcaggaaat
     6361 atccggggaa aatctttcta aaaaatttga cagttttaag aaattgcata attatattca
     6421 aattacacag gatcagccaa aagttgccga ggaagttcaa agagtatcac gtatttctca
     6481 aaacataagt caattgtata aaaattctgt ttatcagata tttgaatttg gtttcattga
     6541 aaaagaaaat caaaaaaatt tattaaagct gctgtctgat tttaaaaaaa tgaaggatcc
     6601 actttcgtct gagatagatg ctcgtaaagt tagaaggcag atcgcacggt tttaccatga
     6661 tatttttgcc aaagtttttg ttcgcagtca aactgagtcg gtagttcctc cgcctgtgaa
     6721 actcatgttg cgttttggtt tttttgatga aacaatgatt gaacaggagc aattgaatga
     6781 actgcatgaa atatcaatgc gcagggttga gcagacacaa acaccaatag tatatgagga
     6841 tgagtttttg accaggatat ataatggtga tgaaaatccc agcatcaatg agatgggttt
     6901 atcgtatgaa gctcatttgc gtgaggagca aaaacatata aaaggcagta gcaatattga
     6961 tgcaacagat aatgtaaaaa aaataattta tgaaattaat catcgtattg ctacagcaac
     7021 atcagtgtgt tcaggttcaa cggcaacatc gtttcctata cttacatcgt tgaatatgaa
     7081 gggtagtatt tcacattatt atgtttcaaa gaaaaagtta gaatccattg taagtgcttt
     7141 gcgtgatgtt gatttttctg ttttttatcg tgaaactgct gtaatgcttg gaaacaccaa
     7201 agaacttata aaagaagaag tagttccgta tttcatttta ttgccatcgt ttggatcaaa
     7261 aacaatgtta tggcaggagc ttgatggaac aaaccgcaga accagagggc gtattgtagt
     7321 gcctatcctt tttatgggtg atatgatgcc aaccatggcg catacatttg ctacgttcag
     7381 gtgggaactc aatcgaacca tcatgggagg tatgtgggcc gatccaatag aaggtggtct
     7441 tactggagca tattttgatt atattaatac atttaaaaag aattctaagt tatcattaga
     7501 agcaaagcaa aaagtggtgg agcgatttaa aagtatccgc aataaccgtg acaggtttgc
     7561 ggatgactac atgcagtggg ttttatatga aaaagatggt atcatgaagc taaataatgt
     7621 tgtacgggat atgttttaca ggcatatacc tttcaaaaag gaattgcgtg ataggttaga
     7681 aaatatgcct gcatataccg aaatagccgg cagattccgt aatgtattta accgggaagt
     7741 aggcaattat gaaaggaaat ttaaaaaata tcaacgtgaa gatggcacat tacctgaggc
     7801 tttgcagaaa tttatggagt tcctatataa ataattgcta tgaattcaca tcgtattaca
     7861 gggataataa ggcattatac cagaaaagcc agagatcaga tatataatgc tggcttaatt
     7921 atttttaaag aagctggcat tgtagagcat ttagatgata tttcagcatg tgttgatgaa
     7981 ttaataaaaa atgctgttaa ggcaaattat aaacatcttc tgattattca ggcgttacag
     8041 cgtcaattcc aattacaata taaagataaa agtcagtccc agatacatga atatatcagc
     8101 gatatcctta aagataaaat tcaatttgat acattagcaa gtattgtttt aaagaaagaa
     8161 aatattgctc aaacggtaag agaagtttta aatcaggaag gaatattttt atcaataaag
     8221 aacagggcat atcaagaaaa cagaacatta cgtaaggatg agttagatgc tatcaataca
     8281 ttacagcttt ttttagattt acaggatacc atgaaaaaga aaaatataca aatattgtta
     8341 aaagtcgact atcgtgatga ttatatctat attgaaataa caaacactgc acctatatta
     8401 tcacatgatt tacaacgaat atacgataaa agagaagagt accggaaata tagaaatgaa
     8461 ggcagggaat atgaattctt cattaataat atcgatacca gcgattcggg ttttggtttg
     8521 gggtatgcaa agattgatag ttatttatat aatttaggta ttgaaccgga taaagccgtg
     8581 actgttatat ctattaataa tactactgcg atgctctcta ttccagttga agctttaaaa
     8641 aaactgtgat agtgtatgtt ttcataattt cgtaattgtc ttttatgatt gatagtagct
     8701 atataatcat tgaataacag aatacacctt tgatatattc aagaactgtt ggggcataaa
     8761 agcagtaaga ccacagagat atatgctcat gtaagtaata gagattgagg taagataaaa
     8821 agtccacttg actgtatatt aaattcagag gtaaaagaaa atgaatgatg ttcaggcaaa
     8881 tggtggtata gacgacatgg atgtcgtata tgaaggagtt atgtgcaata ccttgcccac
     8941 ctttttgaag agcaccggcc atccatgtcc gactaaagca tgaaaaagta gtaattgaca
     9001 aaaaaaatta aggaattatt atgtcgtatc aatttttgag gaaaaacgat gccaacactg
     9061 caatttaaag ggaaaaatat aatctggaat caccaccttt ctattccata ccatacattg
     9121 gatgaagtag atgaactgca ttataaaccg gaaaaggcga atgggaatat tataatagag
     9181 ggtgataatc ttttagcttt aaaagcattg cttccacagt attctggaag gattaaatgc
     9241 atttatattg acccaccgta taatactgga gaagaaaatt gggtgtataa tgataatgta
     9301 aatagtccac ttattaaaga gtggcttggt aaggaggttg ggactgattg tttagttcgt
     9361 catgataaat ggctttgtat gatggtgcct agactgaaat tgttaaaaga actattaaca
     9421 aaagatggta ttatttttat atccttaaat gatattgaga atcactattg caaagtcata
     9481 tgtgatgaaa tatttacaaa ggaaaatttt gtcgcaaata ttatatggga aaaaagaact
     9541 gctagagatg ctagaagagc attttcaata aatcatgatc acatattggt ctatgctaat
     9601 gattatgaaa gtttttataa ttcaagaaac ctgctacctg taactgatga agtattatct
     9661 agatattcga atcccgataa cgaccctcgt ggaccttggc aatctgtagc gatgactgca
     9721 caagctggtc atgcaaccaa agatcaattc tatacagtaa taacgcctac aggaaaaaag
     9781 cataatccgc caaagggttt atgttggatt tttacagaga aaaaatttaa agaagaagtt
     9841 aaaaataaca atatttattt ccccaaaaat ggcgacggag tcccaagaca aaaaaagttt
     9901 ttagaggaga gaggtgatac aggtttagtc ccattaacga tttggacaag caaagaggtt
     9961 ggcacaaatg attcagcaaa aaaatcactc ttagaaatat ttcatgatga attgcatttt
    10021 gataatccaa aacctgttga gttacttcgt agaataataa atatctcaac ttcaagtagt
    10081 gatataatat tagattcctt tgccggttcc ggcactacca tgcacgctgt catggactta
    10141 aacaaggaag acggcggcaa tcgtcaatgt atactcgtcc agatgacaga agcaacggaa
    10201 gcagaaccgg aaaagaacat ctgtaaagac attacccgtg aacgtataaa acgcgcaatt
    10261 gataaatacg gctatgattc tggttttaaa tactttcgtg ttggaatacc aattgacgcg
    10321 gaaagcttat tatctggaaa acttcccact tttaagcagt ttgccgaata cgtttattat
    10381 ctgtgtacag gcgaacatat taaagacaaa tctgttatca acgaaaaaac ctatcttgtt
    10441 ggttcaataa ataattcagt aatttatctc gtttacaagc aaaacttcga tgaattaaca
    10501 agacttgctt taaatctgcc tcttgccgag aaaatcatag cagacaatcc tagaaaaaaa
    10561 attattgtat atgctccggc atgttttctt gaagaagatt acatgaagga aaataatatt
    10621 gaatttgttg gtattccata caaccttttt agaagggaaa atggagcgta gaaaatgttc
    10681 ttaaaaaaat accagcaaaa agtagtaacc gaattaaaat ttttttattc aaaagcaaga
    10741 gagacaaaag atgccttcga tacagcgcga aaatctttgc ctgagaatat gcgacatacc
    10801 cttaactggg ttcaaacaac ctttcagaat acgaacaaag agtataaaga ccgctgtaca
    10861 aatggactaa gtgaatatta cccccgtatc gtcataaaag tacctaccgg cggtgggaaa
    10921 accctgcttg ctgtcgaggc aatccgtgaa tatcagaata tattcgcccg aaaaagaact
    10981 ggtcttgttg tttggattgt tccaagtgaa acaatatatt cccaaactgt tcagaaaatt
    11041 cgagacaaat ctaatccttt acgacaatta ctcgaccagg cgagtggtaa taaaacttta
    11101 gttatcgaaa aaggccagcg gctcaccata catgatattg aagaacattt ggtaatactt
    11161 tttgtaatga tacaatccat aagccgtaag aatggcaagg aagctcttaa agtgtttcag
    11221 gattccggag gttttgagag ttttttcccc gccgataatc gtaacgattt gcattctgaa
    11281 ttgttaaagt ctttcccaaa tctcgattat atagctccta ttggttcaat gttcccatta
    11341 attaagacca gccttggtaa tgccgttcgg gtatcacgtc catttattat aatagatgaa
    11401 atacacaaag ttttctctga tacagcacgg aaaacaatag ataatcttaa cccagaaatg
    11461 gttctcggtt taacagcaac tcccagagtc gaaatgaata tacttgtttc tattaccggg
    11521 a
//
LOCUS       DAOOJH010000075        11383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5196_length_11383_cov_9.159251, whole genome shotgun sequence.
ACCESSION   DAOOJH010000075 DAOOJH010000000
VERSION     DAOOJH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5196_length_11383_cov_9.159251"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..278
                     /locus_tag="PLJ75_09445"
                     /pseudo
     CDS             84..278
                     /locus_tag="PLJ75_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            complement(303..386)
                     /locus_tag="PLJ75_09450"
     tRNA            complement(303..386)
                     /locus_tag="PLJ75_09450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(350..352),aa:Leu,seq:cag)
     gene            complement(458..3214)
                     /locus_tag="PLJ75_09455"
     CDS             complement(458..3214)
                     /locus_tag="PLJ75_09455"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD05478.1"
                     /translation="MFGKYIELKKKYYNSSYKIIAFTFINIAFSLFFLIIYFYRRKEK
                     YYLFFSILSFSIGLWLLGMNGYILHLLDNRIIYYISTYVISCFILISIMLFIHEILGI
                     RKIFLVKLIIGLLIFEVIWIIVELLITGYIYYFSKYFYMIHITLTLLITLCYTGLCVL
                     ASFKNIPYSKMITGGILLLSIPSLIAVVDFLRIYRIETPIAEGFFLMSMSFAAVLASR
                     YAHVFNSLEKANADLLILDKMKDDFLATTSHELKTPLHGIMGLSQTLIDGSPDSVTSA
                     QRENLELICSEAKHLYDMVSEILDFSKIRAGKVDLFLEYTRTEEVASTVVSLLSSEAE
                     KKGITLKLECGDVPIIIADRKRLRQVLINLVGNAIKFSDGGTVTVTIQPKDDRGVRIE
                     VCDEGRGIDTKDLDRIWNPFVQAEDPDIRVAGGTGLGLAISKNLVELHGGSIRVESEL
                     GKGSRFIVDLPESPGMRGISRRRVEKTQDIVTIKKDVRFESDEKENNTLAQLQSFASA
                     RILAVDDDEVNLRVMKGFCDVAGYEFITATHGHEALEILATQDVDIVLLDLMLPGISG
                     YEVCKKIREDAKTMGIPVIMVTAKDTTTDLVRGFITGANDYITKPFSRDELLVRIENQ
                     LAIKQMLDAERSIIRELRTESEGHENLYRRSVDMKQMTLQMVAWERLIRDDMEIARAF
                     QDRLMSHHRNVPGIETYVYYRPLMKLGGDIYDIIEVRPGVLRVFLADATGHGITASLN
                     TVKILSEYSSVKQVMQTPEEILNFLNHRFIELYRDYKIIFTCIIADIDIVHGELSLAS
                     AGHPQSYLLSEGKVTPLKPQGPIICLSSNFEYRQMVMPINNRDLLIFYTDGLLELIGS
                     QYRGAEGPGFDTGKLLEEQIASISKSNSLEEICKSFVAGAESVSTTMNRGSDDITIVA
                     IRKL"
     gene            complement(3733..3951)
                     /locus_tag="PLJ75_09460"
     CDS             complement(3733..3951)
                     /locus_tag="PLJ75_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05479.1"
                     /translation="MSDIRTIIDFVIEAAHNPDLRDEFKKFLYINPDFIKIKAWFDER
                     SYIVSDDECIRILENKDRIFSSIQSKNY"
     gene            4647..5504
                     /locus_tag="PLJ75_09465"
     CDS             4647..5504
                     /locus_tag="PLJ75_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05480.1"
                     /translation="MLFFLYYAGLKPGDTLDFFHNYYSPIWSIIPHILKKQENTLFSE
                     ILINHAAGTHAMAMLLHSVDDHINDGDIPSNHLTLLIRSQAWKLLHDHCEPLTAVISN
                     GKELVDDCLNRYYRSIIAQTCPQNLEEYCNLFRHQMATAFIVPLLLAHDDGNSVLANA
                     VQTALESFGISWRILDDIKDLEIDFLAGSISAVSLSLPEEGKHLWNAIKNSKNRNYKN
                     QSKRTVKNEHQFQTLERIVQEYGIIPALVKKILNEIDNAINHAEEAGMPQFAAELRLI
                     ASPLWEQIP"
     gene            5486..>6510
                     /locus_tag="PLJ75_09470"
     CDS             5486..>6510
                     /locus_tag="PLJ75_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05481.1"
                     /translation="MGTDPMNIPQDIQKAISKESLSLEVTTRCTNACSHCFARAGLEE
                     FPELDHETALSILGEGQSIGYRHLHITGGEPLFWLPLFEVLKEARTMGYESIFMNTNG
                     TLIDRQSAQRLAAIGNVTVSISLQGPPSINDAFRGSEAYQHSSVGIKTAIDAGIPVHI
                     FCVTGKSLLPVLPHFIGQTFSIYPQLIDITLIQLIRVQNDAYDISQELLKPDDFLSLI
                     KMAALLNLFGFPVTFLENPLATVVAHISGMSWLPPTPSLIRPGKLFIMADLRITPAHS
                     IRKSMGLYSPGALSHILSSNEYRHVIDENTQICSACSHVDACRKAGMLRPSEPFRDMM
                     EDIPFCKR"
     assembly_gap    6511..6610
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6611..7764)
                     /gene="lysA"
                     /locus_tag="PLJ75_09475"
     CDS             complement(<6611..7764)
                     /gene="lysA"
                     /locus_tag="PLJ75_09475"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPD05482.1"
                     /translation="MNSFREYFVYRDDQLYCEDVSLADVARIYGTPLYVYSSKCFIDK
                     FTLLDEALASVAHTICYSVKSNSNVSILKLMKDVGCGMDVVSGGELYRAIAAGVNPEK
                     IVYAGVGKNVEEIEYALKTGIQMFNCESLSEIKRINNIAQNNNIVAHIAIRINPDVDA
                     NTHHYITTGKKENKFGISLQYLPELIKSVKSLPHIVLKGIHVHIGSQITQIEPFVEAC
                     KKAIAVIDDIQDMGMHECKTLNLGGGFGIQYNDEPSLDVALWAQNIVESIKKKNLFLI
                     IEPGRFISGNSGALIMQVQYKKKTSHKTFIIVDSGMHHLIRPTLYQSYQYIANTTLRE
                     GEEVVDVVGPICESGDFFAKDRLLTKTEEGDYLAVLSAGAYGMAMASHYNS"
     gene            complement(7761..10457)
                     /gene="greA"
                     /locus_tag="PLJ75_09480"
     CDS             complement(7761..10457)
                     /gene="greA"
                     /locus_tag="PLJ75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPD05483.1"
                     /translation="MENEKIFERVSSMFKEEMWGRLDPKDVGISRFRILDDLFNSVVD
                     AGVLEEVRNMCKEHIVSHPDSIVASYLIGLTGYHLDIIDDKIEMRKLIEMYQKYHKWV
                     IAELLSEKVLEYGESSFALKALATSLERLGRSKEAIPVWENLLKIDRFDAEVAKKLAV
                     ALIDEDKQKSIQYMKLSIEGHIKNGLYDEISDLWNKLVQVSWEDMPFFERIERMLIEA
                     RQYELTSSLLKVLLHKYKDEEHPDQSIQILKKILEYTPNDTAARRELVKLYEKKYGSH
                     SQFQLFLKLSKLTNFKYPVKHAIQDFENSIIFDKGHYVRHRSWGIGRIIDITPNTIVI
                     DFKEKQEHQMSVQMALQALTPLRSDHIQVLKCEDYEGLKKLFDEDLLEFFEILIKSYN
                     GRITVADIKKELIPDFVSEKTWAKWWSSKRSEIRKSPLFGISPEKKDMFIMRDKPLTF
                     ADELLDNFLKTDSFTDKLNVAIEFVNNIEPSEGAAVAQFFVDYFSGELKGDSVTRKIL
                     SYFILSGLSKFIDAKKLKLDQAYKMVVSIVKEYPDIPSISKKIDSYDYKKDFINLIKD
                     AREDWADIYGRVLFETPVRIHKYIVNILIRSHEYGIINKFVEQALISAKEFPEIFLWV
                     AKNLLTKSWDYDWLDYSREQLVLTLFRLMNELKKIELKGNKLKNVAIELLFGGDAAVL
                     KDVVNSFDVQFLGKIFDIAASIPYFEDSMREKFLDIIKSRFPEFNPQQNVIEEEWRID
                     AEILITTPEGFARKQEELNRLINIELATISKELAKVADATGDVRENVEYNSLLEKQSI
                     LKRDINRLENEIKKAQILDMNSISLDKVTVGTVVTISGNGEKETFAVLGPWDADFEKR
                     ILSYRSPIAQALLGKKVGDKVEIRKSEQMKQYTIETIEKYNQ"
     gene            complement(10776..>11383)
                     /locus_tag="PLJ75_09485"
     CDS             complement(10776..>11383)
                     /locus_tag="PLJ75_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HPD05484.1"
                     /translation="LKYVDGGPADLLTEKWSPINKGLEKKKCLGVHFKFRYPGFNSVH
                     LLPPLEVQWDSPGKAVLTYDSRSGKDIQERAIQLPGRAKAISMWVHARGNNYTLEVWV
                     KDYKGDVHVLKMGSLNFVGWRPMKAYIPEYVPQETNSYPQTRVTKIVRFVIRANPEAG
                     NEEVFLFFDQLKVLTDTFEVNFDGQNLHQLMEKGGTQQQQK"
BASE COUNT         3475 a         2421 c         1764 g         3623 t
ORIGIN      
        1 caattatcga caggattaaa aagatagcaa aacccataca tattcaggta tttgtcacac
       61 tcacctgacc atactgtccg ggtgcagttg catccgctca caggctggca attgaaaatc
      121 aaaatatcat agccgacatg gttgaaactt caactttccc acaattagca atcaagcata
      181 atgtttcatc agtaccaaaa gttgttatca atgatacata tgagtttgtg ggagcccaac
      241 caattactgt atttatagat atgattgaga agatttgaga aaaattaccg ttttattaaa
      301 aatgccgcga gagggatttg aaccctcaca ggcgtacacc taccagaccc tgaacctggc
      361 gcgtctacca attccgccat cgcggctttt ttatattagt taattgattc tgtatactat
      421 agttttttgt caaataaaaa aagaaaataa ttattcctca cagcttccgg atagcaacaa
      481 tggttatatc gtcacttcca cgattcatgg tagtactcac tgactcagca ccagcaacaa
      541 aggatttaca gatttcttca agcgaattag atttgcttat tgaagcaatt tgctcttcca
      601 ggagcttccc tgtatcaaag ccaggacctt cagcaccacg atactgcgaa cctattaatt
      661 caagaagacc atctgtataa aaaatgagta gatcacggtt attaataggc ataaccatct
      721 gacggtattc aaaatttgac gataagcaaa ttataggacc ctgcggtttt agcggtgtca
      781 cttttccctc tgataacagg tacgattgag ggtgtcccgc cgaggcaaga gaaagctcgc
      841 catgtacgat atcaatatca gcaataatgc atgtgaaaat aattttgtaa tcccgataca
      901 attctataaa acgatgattg agaaagttta aaatttcctc tggtgtttgc atcacttgct
      961 tcacagacga atattctgaa agtatcttta ctgtgttaag ggaggctgta atgccatgcc
     1021 cggtggcatc tgcaaggaaa accctgagaa caccaggcct cacctcaata atatcgtaga
     1081 tatcgcctcc aagtttcata agtggtctat agtacacata cgtttctatt ccaggaacat
     1141 tacggtgatg cgacatgagc cggtcctgaa aagctcgagc aatctccatg tcatcccgta
     1201 taaggcgctc ccatgcaacc atttgtaagg tcatctgctt catgtccacg cttcgccggt
     1261 aaaggttttc atgtccttca ctttcagttc gcagttctct tattatggag cgctctgcat
     1321 ccagcatttg ctttatcgca agctgatttt ctatcctgac aagaagttca tcccggctaa
     1381 agggctttgt gatatagtca ttggcgccag ttatgaaacc acgcaccagg tctgttgtgg
     1441 tatcttttgc tgttaccata ataacgggga ttcccatggt ttttgcatct tccctaattt
     1501 ttttgcacac ctcataaccg cttatcccgg gaagcatgag gtctaacagc acaatatcaa
     1561 catcctgcgt tgccagaatt tccagggctt catgtccatg agtagcggta ataaactcat
     1621 agccagctac atcgcagaag cccttcatta cccgcagatt aacctcatca tcgtcaacgg
     1681 caaggatgcg agcggaagcg aaggattgca gttgtgcaag agtattattt tccttttcgt
     1741 cagattcaaa acgaacatcc ttttttatag taacaatgtc ttgtgtcttt tctacccgcc
     1801 ttctacttat cccccgcatt cccgggcttt cgggaaggtc aactataaat ctactccctt
     1861 tccctaattc actttcaacc cgtatacttc ccccatggag ttccaccagg ttttttgaaa
     1921 ttgcaaggcc aagtcccgta ccaccagcaa cgcgaatgtc aggatcctct gcctgcacaa
     1981 aggggttcca tatccggtca aggtcctttg tatctattcc cctgccttca tcgcaaactt
     2041 caatccgtac tcctctgtca tctttgggct gtattgttac ggttactgtc ccgccatcag
     2101 aaaactttat ggcattacca accagattga taagtacttg cctcaggcgt ttcctgtcgg
     2161 ctataattat tgggacatca ccacactcca atttcagcgt tattcctttc ttctctgcct
     2221 ctgaagaaag aagcgacacc acagttgagg ctacttcttc tgttcttgtg tattctaaaa
     2281 agagatccac tttcccggca cgaatcttgc tgaaatcgag aatctcactt accatgtcat
     2341 aaaggtgctt tgcctcgctg caaataagtt caagattctc cctttgagca gaagttaccg
     2401 aatccggaga accatctatc agagtctgag ataatcccat gataccgtga aggggtgttt
     2461 taagctcatg gctggttgta gcaaggaagt catctttcat tttatctaat ataagcaaat
     2521 cagcgttagc cttttcaagg gagttaaaga catgtgcata gcgagaagca agcaccgctg
     2581 caaatgacat gctcattaaa aaaaatcctt ccgcaatggg tgtttcaatt ctatatatcc
     2641 gcaaaaaatc aacaacagct atcagggatg gtatagataa taacaatatc ccacccgtaa
     2701 tcattttgga atatggaata tttttaaaag atgccaaaac acacaaacca gtataacata
     2761 atgttattag caaagtcagt gtaatatgaa tcatataaaa gtatttactg aaataatata
     2821 tatatccagt gatcagcaac tcaacaataa tccatattac ttcaaatatt aataatccaa
     2881 ttattaattt gactagaaat atttttctaa tacccaagat ttcatgaata aaaagcataa
     2941 ttgaaattaa tataaaacat gaaataacat aagtacttat ataataaatt atacgattat
     3001 ccaacagatg aagaatatat ccattcatcc cgagaagcca taagccaatt gaaaatgata
     3061 aaatagaaaa aaataaataa tatttttctt ttcttctata aaagtatata attaagaaaa
     3121 ataaagaaaa agcgatgttt atgaaagtga atgcaataat tttatacgag ctgttataat
     3181 attttttttt gagttcaata tatttcccaa acataggagg gcgaaaaaaa ttacaatcat
     3241 aacccaatgc gcttgatcta attgcaatga cattattttt attttgcacc aagtgatgtg
     3301 ggataggtat gatatcaggt ttaccgggta tttgttcaaa aactccgttt ataagcttcc
     3361 ttgactcgaa tattaaatgg ccattgaaaa aaaattgtgc tcctccaaaa tgaattggca
     3421 ctaatattgc atattcttca aaaatattat ctattgttaa atgaaatcta taccaaacat
     3481 ttcctttatg atacttatac ttgttattta cacaccataa atactgaatg tgtaatttat
     3541 cccattttga atcatcaata tctatatctt tatttgatat attgtctgtt gtgctaaaaa
     3601 gccactcata ggataagtcg taattagctt cttcttcctt aacaggattg gtagcactgc
     3661 atgaaatgat aaacatcaac aaactgaggc ttattactat gcaatttttc atcaggtcta
     3721 atttcaatga acttaataat tttttgattg aatactcgaa aatattctat ctttgttttc
     3781 taatattctt atacattcat catcagatac aatataactc ctttcatcaa accatgcttt
     3841 tattttaata aaatcaggat ttatatacag aaacttttta aattcatctc ttaaatctgg
     3901 attatgcgca gcttcaatta caaaatcaat gattgttctg atatctgaca tatgggaacc
     3961 tccattttgt gaaaatcata taaatattaa tgcatgctgt caagattttt ctattttcat
     4021 taaataaaaa tattttacca atacatagtc acttccaata gatttcggaa aatgaatact
     4081 tgaaaaagca tggtacacct gtcagagtta taatttgaac aattaacatt tggaggaaat
     4141 aattttatta gaattttatt gctatatatg aatataacaa aaagaaaaat ttttaaacca
     4201 aatttactat aaatacttta tttaataaat ttaatccgga ttataattaa aaatacaggt
     4261 tagcgaagaa ttatttatat accctgacag tctgataata ttcttcagga catcaatgta
     4321 ttcagatagt gtacacaagg tggtgcttca ttgctttttt actactttaa taattttatc
     4381 aggtaatttt tacattcaaa tttgaatttg gactgtaaaa catatatctt tttgaaaaaa
     4441 aattgacata ggttataata cctttaatct tcctcaatat gacaatctac cgcaaaacat
     4501 cgcagggata gctcaaactt ttttgtaatt cagtcgattt tggccgatat ccactcgaca
     4561 caccttgcac cgctttctac gaacttctga acagtgcggt aatcgacctt attcgcacaa
     4621 ttccttattc cacacagtta cctgcaatgc tgtttttctt atactatgca ggtttaaaac
     4681 ccggcgatac attggatttt ttccacaatt attattcccc catctggtct atcatcccgc
     4741 atatacttaa aaaacaggaa aataccttat tttctgaaat tttaatcaat catgctgccg
     4801 gaacccatgc aatggctatg ctcctgcact cagttgatga ccacattaat gatggcgaca
     4861 tcccatcaaa ccacctgaca cttctcattc gaagccaggc gtggaaactc cttcatgacc
     4921 attgtgagcc tctgacagcc gtaatctcta atggcaaaga gttagttgat gactgtttaa
     4981 atagatacta ccgcagtatc atcgcacaga cttgccccca aaacctggaa gaatattgta
     5041 atctttttcg tcatcaaatg gcaacagcct ttatcgtccc gcttcttctg gcacatgatg
     5101 atggcaattc tgtactcgcg aatgcagtac agacagccct tgagagtttc ggtatttcat
     5161 ggagaatttt agacgatatt aaggacttag aaatagactt cttagccgga agcatttctg
     5221 ctgtaagtct cagcctgcca gaagaaggca aacacttatg gaatgctata aagaactcaa
     5281 aaaatcgtaa ttataaaaat caatcaaagc gtacagtaaa aaatgaacat caattccaaa
     5341 ctcttgagcg gatagtacag gagtatggca taatacctgc acttgtaaaa aagatactga
     5401 acgaaataga caacgcaatc aaccatgcag aagaagctgg catgccacag tttgctgcgg
     5461 aactccgctt aattgcatcc ccgttatggg aacagatccc atgaatattc cacaagatat
     5521 tcaaaaagca atctctaagg aaagcctctc ccttgaggtt accacgcggt gtaccaatgc
     5581 ctgttcgcat tgtttcgctc gcgcagggct ggaagaattc ccggaacttg accatgaaac
     5641 tgcattatca atccttggcg agggacaaag tatcggctat cgccatctgc atatcaccgg
     5701 cggagaaccg cttttttggc tccctctttt cgaggtgctt aaagaagcac gtactatggg
     5761 atacgaaagt attttcatga atacaaatgg aacccttatt gataggcaat ctgcacaacg
     5821 ccttgcagca attggtaatg ttacagtatc aataagcctt cagggtccac catccattaa
     5881 cgatgctttc cgcggctctg aggcatacca gcactcatct gtgggcatca aaacagcaat
     5941 tgatgcaggc attcctgtgc atattttttg cgttaccgga aaaagcctcc ttcccgtgct
     6001 tccacacttc attggacaga ctttcagcat atatccacag ttgattgata tcaccctcat
     6061 tcagttaata cgcgtacaaa acgatgcata tgacatttct caggaattat tgaaacctga
     6121 cgacttttta tcactgataa aaatggctgc gctgcttaac ctgtttggct ttccggtaac
     6181 gtttttagaa aatccccttg caaccgtagt cgctcatatt tccggtatgt catggcttcc
     6241 accaactcct tctctcattc gacctggaaa gctttttatc atggcagacc ttcgtatcac
     6301 acctgcacac tccatcagaa aatccatggg gttgtactcc cctggtgcgc tatctcacat
     6361 cctgtcgtcc aatgaatatc gtcatgtgat tgatgaaaac acgcagatct gttcagcatg
     6421 ttcccatgtg gacgcctgcc gtaaagccgg catgctccgc ccctcagaac ccttcaggga
     6481 catgatggag gatattcctt tctgcaaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn gaattataat gtgaagccat tgccatacca tatgcaccgg cagacaaaac
     6661 agcaagataa tcaccttctt cagttttcgt taaaagccta tcttttgcaa aaaaatcacc
     6721 agactcacag attggaccaa caacatcaac cacttcttcc ccttcgcgca gtgtggtatt
     6781 tgcaatatac tgatatgact ggtacaatgt tggccggatt aaatggtgca tccctgaatc
     6841 gacaataatg aatgttttat gcgaggtttt ctttttatac tgaacctgca ttatcaatgc
     6901 accgctattg cccgatataa accgtcctgg ttcaattatc aaaaaaagat ttttcttttt
     6961 tatactttca acaatattct gtgcccacag ggctacatcc agtgacggtt catcattgta
     7021 ttgaattcca aaccctccgc ctaaattcag cgttttgcac tcatgcatac ccatatcctg
     7081 tatgtcatca atgacagcaa tagctttttt acacgcttcc acaaacggtt ctatctgtgt
     7141 aatctgggaa ccaatgtgca catgaatccc tttcaatact atatgtggca acgattttac
     7201 gcttttaatc aactcgggga gatactgtaa agaaatacca aacttatttt ctttttttcc
     7261 tgtagtaata taatggtgag tattagcatc cacatcagga ttaattcgta ttgctatatg
     7321 cgctacaatg ttattgttct gggcaatatt attaattctt ttaatctcag acaacgattc
     7381 acagttaaac atttgtattc cggttttaag ggcatattca atttcctcta cgtttttacc
     7441 aacacctgca tacactatct tttcaggatt tacgcctgcg gcgatagctc tatacaattc
     7501 gcctcctgaa acaacatcca taccgcaccc gacatctttc atcagcttta aaatgctaac
     7561 attggaattt gacttcaccg aataacaaat agtatgtgca acagatgcta aagcttcatc
     7621 cagcagggta aacttatcaa taaaacattt tgatgaataa acgtataaag gcgtcccata
     7681 tattcttgct acatcagcaa gggaaacatc ttcacaatat aactggtcat ctctataaac
     7741 aaaatattct ctgaaagagt tcattgatta tacttctcaa ttgtttcaat agtatattgc
     7801 ttcatctgtt cactttttcg tatttcaacc ttatcaccaa ccttcttacc taacaatgcc
     7861 tgcgctatgg gtgatcgata cgataatatt cgcttttcaa aatcagcatc ccatggtccc
     7921 agtacagcaa atgtttcttt ttcaccattt ccggatatgg tcacaacagt accaacagtt
     7981 actttatcaa gagaaattga attcatatca agaatctggg ctttcttaat ctcattttca
     8041 agcctgttaa tatctcgttt taaaattgat tgtttttcta acaatgaatt gtattcaaca
     8101 ttttccctca catcgcctgt tgcatccgcc actttagcaa gttcttttga aattgtagct
     8161 aattcaatat ttatcaatct attcaattct tcttgtttac gggcaaatcc ttctggagtt
     8221 gtaatgagta tctctgcatc aattctccat tcttcttcaa taacattttg ctgtgggtta
     8281 aattcaggga atcgcgattt tattatatca agaaactttt ccctcatcga atcttcaaaa
     8341 tacggtatgc ttgcagcgat gtcaaatatc ttccctaaaa actgcacatc aaaactattt
     8401 acaacatcct tcaatacagc agcatcaccg ccaaaaagaa gttctattgc tacatttttt
     8461 agtttatttc ccttcagctc aatcttctta agttcattca ttaatctaaa caatgttaac
     8521 acaagctgtt cacgggaata atctaaccaa tcataatccc atgattttgt aagcaaattt
     8581 ttggctaccc ataaaaatat ttcagggaat tcttttgcac taataagtgc ctgttcaaca
     8641 aatttattta taatcccata ttcatgcgag cggattaata tatttactat atacttatgt
     8701 atgcgcaccg gagtttcaaa caaaacccgt ccatagatat ccgcccaatc ttcgcgtgca
     8761 tcctttatga gatttataaa atccttttta taatcatatg agtctatctt tttggaaata
     8821 gagggtatat ctggatactc cttcacaatt gagactacca tcttatatgc ctgatcaagt
     8881 ttcaatttct ttgcatctat aaatttgctc aaaccactta aaataaagta tgataaaatt
     8941 tttcttgtta ccgaatcacc tttcagttcc cctgaaaaat aatccacaaa gaattgagca
     9001 actgctgctc cttcagatgg ttcaatattg ttaacaaatt ctatagctac attcagctta
     9061 tcagtaaatg aatccgtttt caggaaatta tcaagcaact catcagcaaa ggtaagaggt
     9121 ttatcacgca tgataaacat gtcctttttt tctggtgaaa ttccaaataa tggggatttt
     9181 cgtatttctg atcttttgct gctccaccat tttgcccagg ttttttcact aacaaaatca
     9241 ggaatgagct ctttcttaat atcagcaacg gttattcttc cattatacga cttgattaat
     9301 atctcaaaaa attctaagag gtcttcatca aaaagtttct ttaatccttc ataatcttca
     9361 cactttagca cctgaatatg atcacttcta agcggcgtta gagcctgtaa cgccatctgc
     9421 acagacatct ggtgctcctg tttttcttta aaatcaatga caatagtgtt gggtgtaata
     9481 tcaattattc tgccaatacc ccagcttcta tgccgaacat aatgtccttt atcaaaaatg
     9541 atactatttt caaaatcctg tatagcatgc ttcacaggat atttgaaatt agtcagcttt
     9601 gacaatttca aaaatagctg aaactgcgaa tggctgccat atttcttttc atataattta
     9661 acaagttctc ttctggcggc agtgtcattt ggtgtatatt ccagaatttt tttcaatatc
     9721 tgtatggatt gatcaggatg ttcttcatcc ttatacttat gaagtaatac ttttaacaat
     9781 gaagaagtca actcatactg ccttgcttct atcagcatcc gctcaattct ttcaaaaaat
     9841 ggcatatctt cccatgatac ctgaaccaat ttgttccata agtcgctgat ttcatcataa
     9901 agcccatttt ttatatggcc ctcaatagat agtttcatat attggatgct tttttgctta
     9961 tcctcatcaa taagtgcaac tgcaagtttt tttgcaacct cagcatcaaa tctatctatc
    10021 tttagtaaat tttcccaaac agggattgcc tctttacttc ttcccaaccg ttcaaggctt
    10081 gttgcaagag ctttcaatgc aaagctactc tcaccgtatt ccaggacttt ttcagacagc
    10141 agctctgcaa tgacccattt atgatatttt tgatacattt caattaattt gcgcatctca
    10201 attttatcat caataatatc aagatgatat ccggttagtc caataagata tgaagcaaca
    10261 attgaatcgg gatgggaaac aatatgttct ttgcacatat tccttacttc ttccaataca
    10321 cccgcatcaa cgactgaatt gaataggtcg tcaagtattc tgaatctgga tattccaaca
    10381 tctttgggat ccagcctccc ccacatttct tctttaaaca ttgagcttac acgctcaaat
    10441 attttctcat tttccatatt tgcctctttt gaatcataca ttatttatga tttattatca
    10501 tactacacta taactataat cgtccatata tgtaaaattt tctaaccacc tgtaaaaact
    10561 acaaaagcct gcaaagcata cttatactta aacaggttta acttcaaaat tattttaagg
    10621 aaggaaaagc tacttaacct ggtaattaag tataatgtac tattttatca gtaaaaaagc
    10681 aacaactttt atcatttccg tattgttttt ctaaaacaac aaccccatga ttattgatgg
    10741 ggttgttgta aatccatagg taaaatttta tttatctact tttgttgctg ctgtgtgcca
    10801 cctttttcca taagctgatg gagattctgt ccatcaaaat taacttcgaa tgtatcggtc
    10861 aaaaccttta attgatcaaa aaacaaaaag acctcttcgt tacctgcttc aggatttgca
    10921 cgaatcacaa accgtacaat ttttgttacc ctggtttgag ggtatgaatt tgtttcctga
    10981 ggaacatatt ccggaatgta cgctttcatg ggccgccacc caacaaaatt taacgaaccc
    11041 attttcaaaa catggacatc gcctttataa tccttaaccc acacctctaa ggtataatta
    11101 tttcctctgg catgaaccca catcgatata gctttagccc ttccgggtaa ctggatagct
    11161 ctttcctgta tatctttacc tgaacgggaa tcataggtca atactgcttt tcctggacta
    11221 tcccattgta cttctagagg agggagtaga tgaacggagt taaacccagg atatctaaat
    11281 ttaaagtgaa cacctaaaca ttttttcttt tctaatccct tattaattgg gctccacttt
    11341 tctgtaagta gatctgcagg gccaccatca acatatttaa gct
//
LOCUS       DAOOJH010000076        11383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5197_length_11383_cov_8.888242, whole genome shotgun sequence.
ACCESSION   DAOOJH010000076 DAOOJH010000000
VERSION     DAOOJH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5197_length_11383_cov_8.888242"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLJ75_09490"
     CDS             <1..286
                     /locus_tag="PLJ75_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05485.1"
                     /translation="KDIIVQFAHDAFHYLLWLLWYIAFNPVRKRKCDSPVKYRYSSIQ
                     SYVQEGADVGVPIDHHEYFLQLGDSFSERVKRFLQYEEAYRKRYSIAQWV"
     gene            468..4637
                     /locus_tag="PLJ75_09495"
     CDS             468..4637
                     /locus_tag="PLJ75_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPD05486.1"
                     /translation="MNNNKSIFLGIDAGSTALSIVAIDSGKNIVDTVYTFHHGKIHEA
                     IANYCAQFGKTDVCGIGFTSSAAAITGGHEVNSIVAYVTAIKHTLPQIRSCVIVGGEK
                     FGVIEFDDKGNYSQYRGNTSCAAGTGGFLDQQARRLQLDGSHELSAMALKNDGDIPKI
                     ASRCAVFAKTDLIHVQQEGYDITEICDGLCYGVARNIADTLFASHTPAQPVAIVGGVS
                     LNSAVVKHLESIVGVRFHVPELSHIYGAFGAALYVANNTLQNAVEFTFNTTLKNKLYH
                     FPPLAITMSNYPTFNGIESFVHTSLHDVEVDIYKPMNGFVETFIGIDIGSTSTKMALI
                     DKQGTMLAGFYTRTAGKPLEAVQSIFEACDVYAAKHGISIFVKGAATTGSGRKFIGTL
                     IGADCILDEITAHAKAACTLQPDVDTIIEIGGQDSKFTMIKNGNVIFSQMNNVCAAGT
                     GSFIEEQAYKLGCNLAEYATRALNKQSPLSSDRCTVFMERDINYCMSQGYDTDELLAA
                     VLHSVVENYLSKVSGEAKIGNVICFQGATAKNKALVAAFEQKLQKPLHVSQYCHLTGA
                     LGCALEVMELDITQSSFRGIGLYKKSIPVKSEICNLCTNHCKLKVADVDGEVVAYGFL
                     CGRDYHYKKYVSKTKGIFDFSKERNKIVTLSSNEDTAQVGIPYALHLVDEMDLWIDFF
                     KRLNVTVITSSGCDDPVTIGKRIAQAEFCAPIAAYHGHVTYLADRCNYIFTPFYLEKD
                     APSHSRRQYCYYTQFAPSLTSLIPSVSKKVIMPILNYEIQPLNSRRLLYKACKQMGIN
                     ANSIEVMMAFEQAQNTHKENQKKLMKSGKHAIETRKDIAVVLLGRPYNCIPDSMNKNI
                     PSLFVNLGIPVLYQDMIQYNDDDSHAIDELLKAFHWHYAARILQIAHGIAKSDGLYPV
                     LLTSFKCAPDSFVMEYFKRILDEAGKPYLILQLDEHDSTVGYETRIEAAVRSFKNHYT
                     KKLVKTTAIPVVPSVTRETLKGKTVLLPNWDTMASRLLKANLMSEGIDAHILFEDEAT
                     IAKGTRTNTGQCLPLTIIAFECIDYIQKNKLNTANTILWMMDSEISCNIRMYPYYIKS
                     IFDSYGKGFEKVKVFVGDISFKEVSIKAALYTYFAFMFSGLLRRAGCRYRPYEVIQGA
                     TNYAIEQTMHDFEKAFMDRAEWTDALSGMVARFNAIKIKKTNKPKVALFGDLYVRDND
                     VMNQNLIQFIEQHGGEVITTPYNYYAKMIANPYFKKWFREGKYGEIVTGKTLLTTVEL
                     IEQKYYSYFQKLLNEPEPVYTVNFEKVLQQFGVTMYHTGESFDNLIKIFALLHHYPDI
                     SLFVQTSPAFCCPALVTEAMAKRIYEVTGVPIVSITYDGTESFKNDVVVPYLTLLRNK
                     TPQQEMKQKLS"
     gene            complement(4647..5954)
                     /locus_tag="PLJ75_09500"
     CDS             complement(4647..5954)
                     /locus_tag="PLJ75_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05487.1"
                     /translation="MEKTKLHELLLENKKRFFSKIDIGEYIPREIQYTVAPLLNTKEI
                     LFITGIRRSGKSTLLLLLAKDLMKQYNVPIHNLFYFNFEDERCVDFTLSDFSVLYELY
                     LELENPIGKKYFFIDEIQSIPGWERWVNRMYENEDCKFFITGSNTSLLSSDISTTLTG
                     RNIQIVNYPFSFKEFLKCKNVHINDNDVYITETRATIKHLLHEYMQIGGFPEAAKTGS
                     TILLENYFRDILYSDIIARHKIRNSKEIRELALYCISNIATIQSFNAIKKFLKIKSIT
                     SVKKYLQYFEDVYLFYFINLFDYSVKRQIYNPSKLYIADIGLYNAIGFKYSQNIGHSI
                     ENILFNHLIRGYKDIFYWKSKNGLEVDYIIRQGYTITDSIQVTATIENPVVKDREIRA
                     LLSAKKELNPHNLIIITMDDEQTCTIDNTTISVIPLWKWLLFH"
     gene            complement(6092..6394)
                     /locus_tag="PLJ75_09505"
     CDS             complement(6092..6394)
                     /locus_tag="PLJ75_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPD05488.1"
                     /translation="MYRGVATKFRDLLFFTLFAITVTSSVKLAGVLVVFALLLSPVMI
                     ALSLKLRHILITAWIAGIVINLISIGISYILDLPTGYTLVAVNTLAALIISVLKTT"
     gene            complement(6432..6704)
                     /locus_tag="PLJ75_09510"
     CDS             complement(6432..6704)
                     /locus_tag="PLJ75_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05489.1"
                     /translation="MKMKISSYPFILFITLLLSYSSFASTVHFHTSAHHNCPVCIYQQ
                     VDSFDAIDQTAFEYLSVYTNVHCIIKNFPILLIANPVASRSPPVNG"
     gene            complement(6758..7816)
                     /locus_tag="PLJ75_09515"
     CDS             complement(6758..7816)
                     /locus_tag="PLJ75_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPD05490.1"
                     /translation="MSDIYSALQKRLDMYSLGFPETQSKIEITILKKLFSEDDAKLFM
                     ELSPMLEPAESIAARMGKPVQEIQNHLEDMVKRGLLFKKTKDGVAKYGAIPFIHGIFE
                     FQVKRLPKDLAELLEQYYDDGLAKAIATNANMFLHPIPVNESIESKSTVAPFEDAVAI
                     IQQANGIAVADCICRKEKKTLGKGCDKPMETCLMFGSMAEYYIENNLGRKISADEAIA
                     IVKNAQKQGMVTQPGTAQNPAGMCSCCGDCCAVLLSIKRFPKPAEMVFSNYFAEVNDN
                     CTSCGSCADICQMDAIEMNDFAYVNKDRCIGCGLCVSECPADAIHLVLKDNKRIPPKT
                     SGEQMMQMAKQRGILPDG"
     gene            complement(8197..8490)
                     /locus_tag="PLJ75_09520"
     CDS             complement(8197..8490)
                     /locus_tag="PLJ75_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05491.1"
                     /translation="MSTVCITLYLNGHCIESEAKNQLHRLYELILNSYNVTSELENQY
                     KLLYDFITIADFKALRSMDERLNGLMPSICELTKDDEGKIQLQIQGCNLFFNF"
     gene            complement(8503..8844)
                     /locus_tag="PLJ75_09525"
     CDS             complement(8503..8844)
                     /locus_tag="PLJ75_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPD05492.1"
                     /translation="MLIAVPLTQDNIFSLHFGHCNKFAMYDIDENTKSIVKKEEIPAP
                     PHQPGLLPRWLSEKGATCILAGGIGQRAMSLFEEQHVHVVIGIEGTDPDAIVNDYLAG
                     KLKVGANVCDH"
     gene            complement(8859..9740)
                     /locus_tag="PLJ75_09530"
     CDS             complement(8859..9740)
                     /locus_tag="PLJ75_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPD05493.1"
                     /translation="MMFGNNKNSKSNAVKPGMNDKQAIEVDEELKLEENLNKIKHTIL
                     VLSGKGGVGKSTIATNCAVALASRKLKTGLLDIDIHGPSVPTLLGIEKLGLQATNHNT
                     IIPIQYNDYLKVISAGFMLPDNKEAVIWRGPLKYNLIKQFLSDVEWDELDFLIIDSPP
                     GTGDEPLSVAQLVKNPTGAVIVTTPQNVSTIDVKKSITFCHKLNIPVLGVVENMSGFV
                     CPHCGKETFIFRKSGGSEISQEMGVPFLGSIPIDPLIVENSDAGRPFAQSANSNATAS
                     QKHFEEIISSLLQSITV"
     gene            complement(9758..>11383)
                     /locus_tag="PLJ75_09535"
     CDS             complement(9758..>11383)
                     /locus_tag="PLJ75_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05494.1"
                     /translation="LLPDAEVTIIEKGELLSYAGCGLPFYLSGEVHDYKELMSTAAGV
                     VRDTHFFKMTKDITVLNQTLVESIDRNKKTIKAVHTLSGKSIDIEYDKLVLAVGGTPF
                     IPQIKGIDLKGVHFLSTIDDARKIHDEEKNLKGKNAVIIGGGLIGIEVCESFVKQGMN
                     VTIIEKMDRVLPALLDPEMAYHVHNELQANNVTVLLNDAVVEIKGDDQGNVRSVKTNK
                     TEVPSDLVLIAIGTKPNITLAKQAGITIGTTGAIAVNEFLCTSDPDIYAGGDCVENIH
                     LVSGKPIYAPMGSTANKHGRVIANNICGKESTFKGVLGTAICKVFTMNVARTGLSEEQ
                     ARDGGYDFITVLNPSQDKAHFYPDAKLIIIKLIVDKKSRTLLGAQIVGPGDVAKRIEV
                     VITTITNKATIDDIANYDLAYAPPFSPAIDNIIMTAYIALNKLDGITKSISPVEVKKK
                     MGNNEDFILLDVRSPQEYEEIRIDDVRVKLIPLGKLRESLHQLDKSKEIITFCKISLR
                     GYEAQRILEGAGFRNVKFLDGGIVCWPYKKLVK"
BASE COUNT         3505 a         2026 c         2136 g         3716 t
ORIGIN      
        1 taaggatatc attgtacagt ttgcccatga tgcgtttcat tatttgttgt ggttgttgtg
       61 gtacatagcg ttcaatccag tacggaagcg caaatgcgat agtcctgtca agtatcgcta
      121 cagcagcata caatcctatg tacaggaggg tgctgatgtt ggtgtaccga tagaccatca
      181 tgagtacttt ctgcagctgg gagacagttt cagcgaacgg gtgaagcggt ttttgcagta
      241 tgaggaggcg taccgcaagc gttattctat tgcacagtgg gtataaaaaa ggggtcagag
      301 ctaaagaaaa ggaatttata aattactgtc actatttata aaaattatat ttttttattg
      361 attatttatg ctatttatgt atactatcca gaataaacaa aattatgtat gtgtaaataa
      421 atagtactgt aatatactat caatttacta tgtgacaggg tttggctatg aataataata
      481 aatctatttt tttaggtatt gatgctggct ctacggcgct ttccattgtt gctattgaca
      541 gcggaaaaaa tatcgttgat acagtgtata catttcatca tggaaaaatt catgaagcaa
      601 ttgcaaacta ttgtgcccaa tttggtaaaa ccgatgtttg tggtataggt tttacttctt
      661 cggcagctgc tataacggga ggtcacgagg ttaatagcat tgttgcatac gttacagcta
      721 ttaaacatac tttgccgcag atacgtagct gtgttattgt tggtggtgaa aagtttggtg
      781 ttattgagtt tgatgacaaa ggcaattatt cacagtatcg tggcaataca agctgtgcag
      841 caggaaccgg aggattttta gatcaacagg ctcgaagatt gcagcttgat ggttcacatg
      901 aattgtctgc catggcttta aaaaatgacg gtgatatccc taagattgca tcgcggtgtg
      961 ccgtatttgc caagaccgac cttattcatg tacaacagga aggatatgat atcaccgaaa
     1021 tatgtgatgg actatgttat ggtgttgcgc gcaatattgc tgataccctg tttgcatcgc
     1081 atacacctgc gcagccggtt gccatcgtgg gtggcgtgtc gttaaacagt gcagttgtta
     1141 aacaccttga atcgattgta ggtgtaaggt tccatgtacc ggaactatcg cacatatatg
     1201 gcgcttttgg tgctgcatta tatgtggcaa ataatacttt acaaaatgcg gttgaattta
     1261 ccttcaatac aacattaaaa aataagctgt atcatttccc accattagca atcacaatga
     1321 gcaattatcc cacatttaac gggattgaat cgtttgtgca tacatccctg catgatgttg
     1381 aggtggatat ctataaacct atgaatggct tcgttgaaac atttattggc attgatattg
     1441 ggtccaccag cacaaagatg gcactgattg ataaacaggg gactatgctg gcaggttttt
     1501 atacgcgtac tgcgggaaaa ccgcttgagg cggtgcaatc aatttttgaa gcgtgtgatg
     1561 tatatgctgc aaaacatggt ataagcattt ttgttaaagg ggcggcaaca acaggttcgg
     1621 gacgaaagtt cattggaaca ctcattgggg cagattgtat ccttgatgag attactgcac
     1681 atgcaaaggc agcatgcaca ttgcagcccg atgttgatac aatcattgag attggcggac
     1741 aggattcaaa gtttaccatg ataaaaaatg gcaatgttat tttttcgcag atgaataatg
     1801 tgtgtgcggc tggcactggc agtttcattg aggagcaggc atacaagctg ggatgtaacc
     1861 ttgctgaata tgctaccaga gcgctaaata aacagtcgcc gctttccagt gaccgctgca
     1921 cagtatttat ggagcgggat atcaattatt gcatgagtca gggttatgat acagatgagc
     1981 tgttagcggc ggtattgcat tcagtggtgg aaaattatct ttctaaagtt tccggcgagg
     2041 ctaagattgg caatgttatc tgttttcagg gagcaactgc aaaaaataaa gcgctggttg
     2101 ctgcttttga acaaaagctg caaaagcctt tgcacgtttc acagtactgt catctgaccg
     2161 gtgcgcttgg ttgtgcgtta gaggtaatgg aattggatat tacacaatca agttttagag
     2221 gtatagggct gtataaaaaa tcaattccgg taaaaagtga aatttgcaat ctgtgtacca
     2281 accattgcaa gttaaaagtt gctgatgttg atggcgaggt tgttgcgtat ggatttttat
     2341 gcggtcgcga ttatcattac aaaaagtatg tcagtaaaac aaagggtatc tttgatttta
     2401 gcaaggaacg caataagatt gttacattat cttcaaatga agatactgca caggtgggta
     2461 ttccgtatgc attgcacctt gttgatgaaa tggatttatg gatagacttt tttaaacggt
     2521 tgaatgttac ggttattacc agcagtggat gtgatgaccc ggtaactatc ggcaaaagga
     2581 ttgcacaggc tgaattttgc gcaccaattg ctgcatacca tggacatgtg acatatctgg
     2641 cagatagatg taattatata tttacgcctt tttatttaga aaaagatgct ccttcccatt
     2701 caaggagaca gtattgctac tatacacagt ttgcgccatc attgacctcg ttaataccat
     2761 cggttagcaa aaaagttatc atgcctatac ttaactatga aatacaacca ttgaacagca
     2821 gacgattgct atacaaagcc tgcaaacaga tgggaattaa tgctaactct attgaagtta
     2881 tgatggcatt tgaacaggca cagaataccc ataaagaaaa tcagaaaaaa ttgatgaaaa
     2941 gtggaaaaca tgccattgaa accagaaaag acattgcagt tgtattgctt ggcaggccat
     3001 ataattgtat ccccgattcc atgaataaaa atataccgtc attgtttgtt aatttaggca
     3061 ttcccgtcct ctatcaggat atgatacagt ataacgatga tgatagccat gcaattgatg
     3121 aactgctcaa agcatttcac tggcactatg ctgccaggat tttgcagata gctcatggta
     3181 tagcaaaatc tgatggatta tatccagtat tgctgacatc atttaaatgc gcccccgatt
     3241 catttgttat ggagtacttc aagcgcattc ttgatgaggc gggcaaaccg tatcttatat
     3301 tgcagcttga tgagcatgat tcaacggtgg gatatgaaac gcgcattgaa gctgctgtca
     3361 ggtcatttaa aaatcattat acgaaaaagc tggtaaaaac aactgctatt ccggtagttc
     3421 cttcagttac ccgtgaaaca ctcaaaggta aaacggtact gttgccaaac tgggatacaa
     3481 tggcatcccg attgctgaaa gcaaatctta tgagtgaagg gattgacgcg cacatactgt
     3541 ttgaagatga agccacaata gcaaagggaa cgcgtaccaa tacaggacag tgtttaccgt
     3601 taaccatcat agcatttgag tgcattgatt atattcaaaa aaataaactt aatactgcaa
     3661 acactatctt gtggatgatg gattcagaaa tttcatgtaa tatccgtatg tacccttatt
     3721 acatcaagag tatctttgac agctatggta agggatttga aaaagtaaaa gtatttgtgg
     3781 gcgatatctc atttaaagag gtttccatta aggcagcact atacacatac tttgcattta
     3841 tgttcagcgg gttgctccgc cgtgcaggat gcaggtaccg tccgtatgaa gttatacagg
     3901 gtgctaccaa ttatgccatt gaacaaacta tgcacgattt tgaaaaagca ttcatggata
     3961 gggcggagtg gactgatgct ctgtcgggta tggttgctcg ttttaatgcc attaaaatta
     4021 aaaaaacgaa caaaccaaaa gttgcgctat ttggcgatct ctatgttcgt gataatgatg
     4081 ttatgaatca aaaccttata caatttatag aacagcatgg cggcgaggta ataaccactc
     4141 cctataacta ttatgcaaag atgatagcca acccctattt taaaaaatgg ttcagggaag
     4201 ggaaatatgg ggagatagtt actggtaaaa cgttgcttac aacagttgag cttatagagc
     4261 aaaagtatta ctcatatttc caaaagctgt taaatgagcc cgagccggtg tatactgtaa
     4321 attttgagaa ggtgctgcag cagtttggcg taaccatgta tcataccggc gaatcatttg
     4381 acaatttaat aaagatattt gcattattgc atcactatcc tgatatctca ctgtttgtgc
     4441 aaacaagccc ggcattttgc tgcccggcgc tggttactga agcaatggca aaacgtattt
     4501 atgaagttac cggagttccc atcgtcagta ttacttacga cggtaccgaa agttttaaaa
     4561 atgatgttgt ggttccgtat ttaacgttat tacggaataa aacaccacag caagaaatga
     4621 agcaaaagct gtcttagata aacgtattag tgaaacaata accatttcca tagtggaata
     4681 acagaaatgg tcgtgttgtc tatagtgcag gtttgttcat catccatagt tataatgatt
     4741 aaattgtgtg gattaagttc tttttttgca gatagcagtg cacgaatttc tctatctttt
     4801 acaacaggat tttctatggt ggcagttacc tgaattgaat cagtaatagt atacccctgt
     4861 cgtattatat aatcaacttc taatccattt tttgatttcc agtaaaagat atctttataa
     4921 ccacgtatga gatgattgaa aagaatattt tcaatactat gaccaatatt ttgtgaatat
     4981 ttaaagccaa tagcattata taatcctata tctgcgatat agagctttga tggattatat
     5041 atttgccttt ttactgagta atcaaaaaga ttaatgaaat aaaataaata cacatcttca
     5101 aaatattgta aatatttctt aactgaagtt atgcttttaa tttttaaaaa cttttttatg
     5161 gcattaaaac tctgaatggt agcaatgttt gatatacaat aaagtgcaag ttcccttatt
     5221 tctttactat ttctaatttt atgtctggca ataatatcac tgtatagtat atctctgaaa
     5281 taattttcta atagtatggt gctacctgtt ttagcagcct caggaaaacc tccaatttgc
     5341 atatattcgt ggagcagatg cttgatagta gcgcgggttt ctgttatata tacatcatta
     5401 tcgttaatat gtacattttt acatttcagg aattctttga aggaaaaagg gtaatttact
     5461 atttgtatat ttcttcccgt caatgttgta gaaatatcgg atgataaaag tgaagtattt
     5521 gaaccagtta taaaaaattt acaatcttca ttttcataca ttcggttaac ccatcgttcc
     5581 cagccaggaa tactttgaat ttcatcaata aaaaaatact ttttgccgat agggttttcc
     5641 agttctaaat ataattcata taatactgaa aaatctgata gagtaaaatc aacacatcgt
     5701 tcatcttcaa aattaaaata gaacaaatta tgtattggta cattatattg tttcattaaa
     5761 tctttagcta gaagtaatag caatgttgat ttcccagacc tgcgtatacc cgttataaaa
     5821 agtatttctt tggtattcaa aagaggagca actgtatatt gaatttcgcg ggggatgtat
     5881 tcacctatgt caattttaga gaaaaacctc tttttatttt ccagtaataa ttcatgtagt
     5941 ttagtttttt ccattgatat atatttgaaa tgttatattt gatagtatat attacattag
     6001 aaatgtaata atattgaata aaattgtcaa gtatttttca tgtaatggca tgaatgcatg
     6061 cctgttacat tgtttataga atcaattaat tctatgtcgt ttttaataca ctgattatga
     6121 gagcagcaag cgtattgact gcaaccagcg tataacccgt tggtaaatcc agtatatacg
     6181 aaatacctat ggaaatcagg ttaatgacta tgccagcaat ccatgcagta ataagaatat
     6241 gccttagttt caatgataat gcaatcatta caggtgaaag aagcaatgca aatactacca
     6301 gcactccggc caatttaaca gagctggtta ctgtaatggc aaataatgta aaaaaaagga
     6361 gatcgcgaaa ttttgtagca acacccctat acacgaaatt tttagtttag atttgtgata
     6421 atggttatat attaaccatt gacaggaggg gagcgggagg ctacaggatt ggcaatcaat
     6481 aaaatgggga agtttttgat gatacaatgt acattagtat atactgaaag atactcaaat
     6541 gctgtttgat caatggcgtc aaatgaatca acctgctggt atatgcaaac cgggcaatta
     6601 tgatgagcgg aggtatgaaa atggacagta ctggcaaagc tgctgtatga taacaaaaga
     6661 gtaataaaaa gaataaatgg atagcttgaa attttcattt tcatgctatt aactatacta
     6721 cagggtgata ctaatttcaa aaaaataata ctttagctta tccatcaggt aaaatgccgc
     6781 gttgctttgc catttgcatc atctgctcac cacttgtttt tggtggtatt cgtttgttgt
     6841 cttttaatac aaggtgtatg gcatcagcag ggcactctga aacgcataat ccgcatccaa
     6901 tacatctatc cttattaacg tatgcaaaat cgttcatttc aattgcatcc atctgacata
     6961 tatcagcaca acttccgcat gatgtacagt tgtcatttac ttctgcaaaa tagtttgaaa
     7021 acaccatctc ggcaggttta ggaaatcgct ttatagataa taacaccgca cagcaatcgc
     7081 cacagcaact gcacatacct gcagggtttt gtgcagtgcc tggctgagtt accatgccct
     7141 gtttttgtgc attttttacg atagctatcg cctcatcggc acttatcttt cgtcccagat
     7201 tattttcaat atagtattca gccattgaac caaacataag gcatgtttcc atgggtttgt
     7261 cgcatccttt cccgagggtc tttttttctt tacgacatat acaatcagca accgctatac
     7321 cattagcttg ctgtatgatt gcaacagcat cttcaaaggg tgcaacagtg ctttttgatt
     7381 ctatggattc atttaccgga atcggatgca aaaacatatt ggcatttgtt gcaattgctt
     7441 ttgcaagtcc atcatcatag tattgttcca gtaattcggc aaggtctttg ggtaatcgct
     7501 ttacctgaaa ttcaaagatg ccgtgtataa aggggatagc gccatatttt gcaactccat
     7561 cctttgtttt tttaaaaaga agcccccgct tgaccatgtc ctctaaatgg ttttgtattt
     7621 cctgtacagg ttttcccata cgtgcagcta tgctttcagc aggttcaagc atgggcgata
     7681 gttccatgaa aagttttgca tcgtcttcag aaaaaagctt ttttaatata gtaatttcta
     7741 tctttgattg agtttcggga aatccaagcg agtacatatc aagacgtttt tgcagtgcag
     7801 agtagatatc actcataaca acctccgttt gtcatgtatt gtcatgtaat ggaaacatta
     7861 aattggggac attcaaataa cgctgttatg ttcgtcaaat atattttttt tgattccaaa
     7921 agttttgttg ttatattgga tgtttaaata tttatattag atatacatat ttttaaaaca
     7981 atttattgtt tttataacaa cggctttttt aaggacatgt tatggtaata ttgattttga
     8041 atacaatgta atatatttgt ctcatttaaa acctttataa caacaaaact gccgcctgta
     8101 taaccatctt caattctaaa gggattttca tcatcaaaaa gtgtttgttt aaattgaaag
     8161 ttagaatagc ccgcgttagt cataagatta acgatttcag aagttgaaaa aaaggttgca
     8221 tccttgaatt tgtagctgta ttttgccttc atcatccttt gttaattcac atattgaagg
     8281 cattaaaccg tttaaacgtt catccatgga gcggagtgcc ttaaaatcag ctatcgtaat
     8341 aaaatcatac agcagcttat attgattttc aagctcagat gtaacattat atgaattaag
     8401 aattaattca tatagtctat gtaactggtt ttttgcttca ctctcaatgc aatggccatt
     8461 taaataaagt gttatacata cagtgctcat atttaactca tatcaatgat cacaaacatt
     8521 agcaccaact tttagtttcc ctgctaaata atcgtttaca atggcatcag ggtcagtacc
     8581 ttcaatacct atgacaacat gtacatgttg ttcttcaaat aatgacatgg ctctttgtcc
     8641 aatgccacct gctaaaatac atgtagctcc cttttcagaa agccatcgtg gaagaagacc
     8701 tggttggtga ggcggtgcag gaatttcttc ttttttcaca atagatttag tattttcatc
     8761 aatatcatac attgcaaatt tattgcaatg tccaaaatgt aatgaaaaaa tattgtcctg
     8821 tgttaacggt acagctatta gcatatgcat ttctcctttt atacagttat tgattgcagt
     8881 aaactgctga ttatttcttc aaaatgcttt tgagatgcgg tagcattact gtttgctgac
     8941 tgtgcaaatg gcctgccggc atcactgttt tccacaataa gaggatcaat agggatgctc
     9001 cctaaaaagg gtactcccat ctcctgtgaa atttcactgc cgccactttt cctgaatatg
     9061 aatgtttctt ttccacaatg ggggcataca aatccgctca tattttccac aacacctaat
     9121 accggtatat taagcttatg acaaaaggta attgattttt ttacatcgat ggttgaaaca
     9181 ttttgtggtg ttgtgactat gacagcacca gtaggatttt taacaagctg tgcaacagat
     9241 aatggttcat caccggtgcc tggaggggaa tcaataataa ggaaatcaag ttcatcccat
     9301 tcaacgtcag ataaaaattg ttttattaaa ttgtatttta atggtcctcg ccatataaca
     9361 gcttctttat tgtcgggtag cataaaacct gctgatataa ctttaagata gtcattatat
     9421 tgtataggga taattgtatt atggtttgtt gcctgcaaac ccaatttttc tatgcccaga
     9481 agtgttggaa cacttgggcc atgaatatca atatcaagca aacccgtttt taattttcgc
     9541 gatgcaagcg ctacggcaca gtttgttgca atagtacttt tcccaactcc acctttacca
     9601 gataaaacca gaatggtgtg cttaatcttg ttcaggtttt cctctaattt caactcttca
     9661 tcaacttcaa tagcctgttt atcattcata cccggtttca ccgcattgga ttttgaattt
     9721 ttattattac caaacatcat aacctcctgc aatcatgtta tttaaccaat tttttatacg
     9781 gccagcatac aattccacca tctaaaaatt ttacatttct aaaaccagca ccttcaagaa
     9841 ttctttgtgc ctcatatcct cgtaatgata ttttacaaaa agttattatt tcttttgatt
     9901 tatcaagctg atgcagggat tctcgcaatt tacctaatgg aattagtttt acccggacat
     9961 catcaattct aatctcttca tattcctgag gtgaacgaac atcaagtaaa ataaaatctt
    10021 cattattgcc cattttcttt ttaacttcaa ctggtgaaat ggatttagta atgccatcta
    10081 acttatttag tgctatgtat gcagtcataa taatattatc tattgcagga gaaaatggag
    10141 gtgcgtaagc taaatcgtaa tttgctatgt catctatggt tgccttattt gttattgttg
    10201 taataaccac ttcaatgcgt ttagcaacat caccgggtcc aactatttga gcaccaagca
    10261 atgtacggct ttttttatca acaattaact taataataat taattttgca tcgggataaa
    10321 aatgtgcttt atcctgtgaa ggattaagta ctgtaataaa atcataacct ccatctctgg
    10381 cttgttcctc agaaagtccg gtgcgggcaa cattcatggt aaataccttg caaatagccg
    10441 tacccaatac acctttaaac gttgattctt ttccacaaat attgtttgca ataactctcc
    10501 catgtttatt ggctgttgaa cccataggag catagattgg tttccctgaa accaaatgta
    10561 tattctcaac acaatcacca ccagcgtaaa tatcaggatc agatgtacat aaaaattcat
    10621 taacagcaat ggcacctgtt gtacctatag taatgccagc ttgttttgct aaggttatat
    10681 ttggttttgt accaatggca atcaatacca aatccgaggg cacttccgtt ttatttgttt
    10741 ttacgcttct tacattaccc tgatcatcgc ccttaatttc tactactgca tcgttaagta
    10801 atacagtaac attattggct tgtaattcgt tatgaacgtg ataggccatt tcaggatcaa
    10861 gaagtgcagg gagtacccta tccatttttt caataatggt tacgttcatc ccctgtttaa
    10921 caaaagattc gcaaacttca attcctatca accctcctcc aatgataacg gcatttttgc
    10981 cttttaaatt tttttcctca tcatgaattt ttcgtgcatc gtcaatagtt gaaaggaaat
    11041 gtactccttt taaatcaata cctttaattt ggggtataaa tggtgtgccc cccacggcta
    11101 acactaattt gtcatattca atatctatgg atttacctga tagcgtatgt accgctttta
    11161 ttgttttttt gtttcggtca attgattcaa ccagcgtttg attcaaaacg gtaatatctt
    11221 tcgtcatttt aaaaaaatga gtatccctca caacccctgc tgctgtgctc atcagttctt
    11281 tataatcatg cacctcgcct gaaagataaa aaggcaatcc gcacccggca tacgataaaa
    11341 gttcgccttt ttcaatgatt gttacctctg catcaggaag cag
//
LOCUS       DAOOJH010000077        11313 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5226_length_11313_cov_11.389501, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000077 DAOOJH010000000
VERSION     DAOOJH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5226_length_11313_cov_11.389501"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PLJ75_09540"
     CDS             <1..930
                     /locus_tag="PLJ75_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05495.1"
                     /translation="KKMQKFIFISNDEHKRNELTKQLDCVDCIVWDEQTTTINKWVND
                     DMCTIIDVELLPAIHGIGSVIDEFSRRKKEIIGVSSKSSISEIDKKNIVQYGITAVFP
                     LSQCRYIPPYCDKYQGLVSGIIGIVDNNIFHCQGLSTLIKKFGYDAIVMNDLKDCCSA
                     DNSMDILCINCSQVSTHEIAAMYIGGKLPKKNAMVLYKSHEDDIFIHDILKLNRIVKV
                     IYTLEEVYAVLLQLLFRQQFHALVYSLYESSDMQSSATSYRGNLRQWYWEAGMDIFTM
                     ADVLQPELVSTYQKHIQLMHALMTRAMAFQWMR"
     gene            complement(945..1523)
                     /locus_tag="PLJ75_09545"
     CDS             complement(945..1523)
                     /locus_tag="PLJ75_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HPD05496.1"
                     /translation="MKIRIFITALILCLAFIGAQKNVSFVGVSVCKKCHESEAIGNQY
                     KFWLQSPHAKAYLLLKQDKALELAKELCIQNPHSNELCLKCHTTGFGKNPALWDDGVG
                     CESCHGPGSIYSSFENHVSFDNRETAYKKAVSKGMYPIRGTDGIKAREKVCRSCHNEK
                     RPCYPKDPALQQHQFLPLQLIADFVFPHRLRR"
     gene            1678..1959
                     /locus_tag="PLJ75_09550"
     CDS             1678..1959
                     /locus_tag="PLJ75_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05497.1"
                     /translation="MDKKHDKRVGRFLVENNIVTEKQLQEALELQHDNPERLIGEILV
                     TLGVLTKEELIMALEMYMMTTDAVPDHVDEWLDQDEIDLLMDKIKQENR"
     gene            2078..2254
                     /locus_tag="PLJ75_09555"
     CDS             2078..2254
                     /locus_tag="PLJ75_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05498.1"
                     /translation="MDIKIQIPEIPEIPEEELTPTVKVLLAIISQCMQIIEAQQHEIQ
                     LLTNEVTRLKRKYH"
     gene            2302..2718
                     /locus_tag="PLJ75_09560"
     CDS             2302..2718
                     /locus_tag="PLJ75_09560"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPD05499.1"
                     /translation="MIEFEDKTCFGCGKSNERGLRLSLKFDPDTKTAYGEFKADQTLE
                     GPPNIIHAGIIASVLDETMMTVNKYMEYIALTGEVTIRYLQPAFIEENLYIRGWFVKK
                     NKRVIENRAEIENEMGKIVARAKAKYIEVEELPQPE"
     gene            2739..2822
                     /locus_tag="PLJ75_09565"
     tRNA            2739..2822
                     /locus_tag="PLJ75_09565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2773..2775,aa:Leu,seq:caa)
     gene            3389..>4846
                     /locus_tag="PLJ75_09570"
     CDS             3389..>4846
                     /locus_tag="PLJ75_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD05500.1"
                     /translation="MPNQMPENLKNIKDILDLDEELHKVSPEAETVGEKSGVRAKKGD
                     INFQLKPEELESYLNKYIVGQEEAVEIIATKICTHFNRMTLEQELPDDEKIVGNIKSN
                     MLLIGPTGVGKTYIIKLIAKKIGVPFVKADATKFSETGYVGGDVEDLVRELVHEADGD
                     VAKAEYGIIYLDEIDKIASSGNVYGPDVSRAGVQRNLLKLMEEADVDLKTPHDLASQV
                     EAAMEAQRTGKVTRKKINTKNILFIVSGAFSGLEDIINKRLNKQAIGFDKSQIRREDR
                     QSVLKQVKTEDLIEFGFESEFIGRLPVTVVLNDLDEEGLYKILKNKYSTVVLGKKLDF
                     RAYGIDLEFMDEALKMLAAKAYKERTGARGLLSVFEKTLIKFEKTLPSTDIKKLVVNS
                     VVVDNPESVLKNILLSDNIRKFQKEFLLKHGVVMEFDGEALQIIQEKAREAKMSIKQF
                     CDDLFHDYPYGIKLMNLDRFVITKGAVENPQSYIDE"
     assembly_gap    4847..4946
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4947..5143
                     /locus_tag="PLJ75_09575"
     CDS             <4947..5143
                     /locus_tag="PLJ75_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05501.1"
                     /translation="DKKRKTNKKATLFDKVFTVIIQYKETSLFRWIYNHPGIAMSVVA
                     LWGIIIIVMFMWFVISLATP"
     gene            complement(5133..6047)
                     /locus_tag="PLJ75_09580"
     CDS             complement(5133..6047)
                     /locus_tag="PLJ75_09580"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05502.1"
                     /translation="MKLLAEGTINTFCQAIGHIGYPSYIIKGYTKSIMIDAGINLLAL
                     KYYSDLLSLFGEPIALDYVAITHSHYDHLGAIPYLLKKIPNVSVVGAKRIQDLLSKQT
                     VIDFMTHLSDIQKPLFADIVNNEDVSLSYFDITCPVKEGDFIDLGDISCVVYEVPGHT
                     RDSLAYFFPEIGMLCAGEAVGVPQGINGEIVQVEFLSSYDDYCKSLEKMLSLKPQTLC
                     MAHGFVYTDDDVTKFLELSITSTKQYKELLLQYLDRVNGDIEKAIELITFEEYDKKGL
                     IYQERNAYISNLSAQVKLVAKEHNYIKG"
     gene            6294..7376
                     /locus_tag="PLJ75_09585"
     CDS             6294..7376
                     /locus_tag="PLJ75_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05503.1"
                     /translation="MNTKKYINITILLGMIASGSLQVFGSDVTISNVRPNLMNIRTTN
                     GKFYAYIVLLFNENPHFVNLIQADSIPVRLMQTTGFNIVQYEAYLKTLQDTSLKTLAQ
                     QVLRVYKEPDKISDNELEQLTQKLENRNIILRFSKTKDGGEYKYLLDYCIYGKRKPVT
                     INHPLFNVKVRIFNIQPLIYYDEFSTSNSTFYFDMIYINPDEVYNDYIISKRIMDNKS
                     CENMFFVGAHVNEDIRFCIKKSFSSKETIFDEIWKMFEIHELTHKILNNYYYFFDQVT
                     GEELALSSTIYYNTYLGLAILYSYLDYNTINPHRIAASNYIKFLSLYTANGEYIEKPS
                     LIKNLPIEDIKKIARLHFETTIKNLK"
     gene            complement(7381..8433)
                     /locus_tag="PLJ75_09590"
     CDS             complement(7381..8433)
                     /locus_tag="PLJ75_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05504.1"
                     /translation="MPGLITHQRIFFESVTSLKKLKHPDHSTHLLDAQFSHPLFMSSA
                     LAGLLIPNLFDYLPGKRDIFFGNSIKHFLHSDDGLSVIFAMFNTLLNNNHEPNEWVYY
                     QKGFFYGYISHWVTDAMYHPFVFYWAGFPTYKDNRVSYYRNQHLLFEYTIDQYMAYFY
                     NSEPFDYNLDRMIPQKNTILYKASKDIFTNAIATIKPSLTLTPSIISWLKHDGYDIAL
                     QCVKPVYSLKKTRNAHLIKILRLLEKRTYSDFLVRYPDARRINRHILNLHRDRWFNPG
                     GEVGLHYESVDDLFRIVKEKTVTLWKQFEELLNHKEMISPSQFNAVIEFTNAYTGKKG
                     FSLDGMIHQNPIRLRN"
     gene            complement(8851..9300)
                     /locus_tag="PLJ75_09595"
     CDS             complement(8851..9300)
                     /locus_tag="PLJ75_09595"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05505.1"
                     /translation="MKPVRYFLPIFIFIFAISCVSSPKNCKEGDCKNGIGTSTHDNGT
                     YNGSFNNSIREGMGEYNFTNGDRYRGNYSNNMRNGQGEYYWKNGDSYKGNWVDGKRNG
                     FGIYTWADGTRYEGEWKDDKRHGKGKLILNDGTTFDGSWENDLFIRK"
     gene            complement(9609..9923)
                     /gene="cutA"
                     /locus_tag="PLJ75_09600"
     CDS             complement(9609..9923)
                     /gene="cutA"
                     /locus_tag="PLJ75_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPD05506.1"
                     /translation="MDIIIMYCTVPDNDVALAIADHLVNNKLAACVNIVSGLMSVYSW
                     KNEICNDSELLLIIKTKKDLYHEVELSIKALHPYEIPEIIALPVVAVSNDYAEWINNV
                     TK"
     gene            complement(9936..10397)
                     /locus_tag="PLJ75_09605"
     CDS             complement(9936..10397)
                     /locus_tag="PLJ75_09605"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPD05507.1"
                     /translation="MDTKFVHDSTITIVQQMTHQDANLAGNVHGGTIMKLIDNTGGIV
                     ASRHSGCLVVTASIDRLDFHSPVYVGDLLRLKASVNFVGTTSMEVGVRVEAENFITGV
                     VRHTASAYLTYVALDEKGKPEKVPALIPETDEEKRRNKEAELRRKKRLLEK"
     gene            10582..11223
                     /locus_tag="PLJ75_09610"
     CDS             10582..11223
                     /locus_tag="PLJ75_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPD05508.1"
                     /translation="MPVNKTELVSFYSHAIAALASFIGLFILIEAAQSYIVKILSAVY
                     AISVIALFSASSLYHGLKRQDNEESLWRKLDHMAIFIMIAGTYTPVCYLYLPATKAFA
                     ILFFQWLLVASGILFKLYFFNAPRIIYTTIYLIMGWMAIVVIKDILHIMDSTSLWYLF
                     AGGIAFTAGAVFYIMQWPKKIQGYVGFHEFFHFFIIAGALCHYLMVYRGVKYM"
BASE COUNT         3736 a         1795 c         2042 g         3640 t
ORIGIN      
        1 aaaaaaatgc aaaagtttat atttatcagc aatgatgagc acaaaagaaa tgaactaaca
       61 aaacaattag attgtgttga ttgtatagta tgggatgagc aaacaaccac gattaataaa
      121 tgggttaatg acgatatgtg cactattatt gatgttgagt tattaccagc catccatgga
      181 attgggtcag ttattgatga gttttccaga cgcaaaaaag agattattgg tgttagctca
      241 aaatcttcaa taagtgagat tgataaaaaa aatattgtcc agtatggtat tactgctgtt
      301 ttcccattat ctcaatgcag gtatatccct ccctactgtg acaagtacca gggacttgtt
      361 tccggaatta taggcatagt ggataacaat atatttcatt gtcagggact atcgacatta
      421 ataaaaaagt ttggctatga tgcaattgtt atgaatgatt tgaaggattg ttgcagtgca
      481 gacaattcaa tggatatttt gtgcattaat tgttcacagg taagcactca tgaaattgca
      541 gctatgtaca tagggggaaa gctcccaaaa aaaaatgcaa tggtattgta taaaagtcat
      601 gaggatgata tattcattca tgatatactg aaacttaacc gaatagtaaa agttatctat
      661 acactggaag aagtttatgc agtattatta cagcttctat tcaggcaaca attccatgca
      721 ttggtatatt ctttatatga atcatcagat atgcaaagta gcgcaacatc atatcgagga
      781 aacttgcgtc aatggtattg ggaagccgga atggatattt ttaccatggc tgatgtgcta
      841 caacctgaac ttgttagcac gtatcaaaag catattcaat taatgcacgc tttgatgaca
      901 agagctatgg ctttccaatg gatgcgatag tcctgataat agtgttaccg gcgcaaacga
      961 tggggaaata caaaatcagc aattagttgc aacggcaaaa attgatgctg ttgcaaagca
     1021 gggtcctttg gatagcatgg tcttttttca ttatggcagc tgcggcaaac cttttcgcgt
     1081 gcttttatcc cgtcagttcc ccgtattggg tacatgcctt ttgacactgc ttttttgtag
     1141 gctgtctcgc gattatcaaa cgaaacatga ttttcaaaac tactatagat gcttcctggc
     1201 ccatggcagg actcgcatcc tacgccatca tcccatagag caggattttt gccaaatcca
     1261 gtggtatggc acttgaggca taattcatta ctgtgaggat tttgtataca tagctccttt
     1321 gccagttcaa gtgccttgtc ctgcttaagt aagagatatg cttttgcatg aggagactgt
     1381 agccagaatt tgtattgatt gccaatagct tcagactcgt gacatttttt acatacggat
     1441 acaccaacaa aagatacatt tttttgtgca ccaatgaagg caagacaaag tatcaaggct
     1501 gttataaaaa ttcgtatttt cataataata ccatagtaac tatctaacta taaaataaaa
     1561 aaatgtaaat aatttttatt gtgggcgata gttattgtca atttttttaa cttgacttat
     1621 ttaatttttg ctattactat ccaatagtaa ataagctaat acagtaatgt tactattatg
     1681 gataaaaaac acgataaaag agttggacgt tttctggtag aaaacaatat agtaactgaa
     1741 aaacagttac aggaagcatt agaattgcaa catgacaatc ctgaaaggct tattggtgaa
     1801 attcttgtaa ctctgggtgt gcttaccaaa gaggagctta tcatggcgct ggaaatgtat
     1861 atgatgacca ccgatgccgt accagatcat gttgatgaat ggctggatca agatgagatt
     1921 gatttattaa tggataaaat aaaacaagaa aacagataac caataacgac ggtaattgat
     1981 ctaaaaagta actgcctcaa aaaagtaact gctcaaaaaa cgtaggtaaa tacaaaaaaa
     2041 gactattaat tttaatgggt actattatca tgtccccatg gacataaaga tacaaatacc
     2101 tgaaatacct gaaatacctg aagaagaatt aacgccgaca gtgaaagtgt tgcttgctat
     2161 aatcagccaa tgcatgcaaa tcattgaggc acagcaacat gagattcagc tattaacgaa
     2221 tgaggtaacc cgattaaaac ggaaatatca ttaaattgaa aagttcttga catttataat
     2281 acgatttaaa agatatgcca catgattgaa tttgaagata aaacgtgttt tggttgcgga
     2341 aaatctaacg aaagaggttt acgtcttagc ttaaagtttg atcctgatac taaaacagct
     2401 tatggagaat ttaaagcaga tcaaacactt gaaggtcctc ccaatattat ccatgctgga
     2461 atcatagcaa gcgttcttga tgaaactatg atgacggtta ataaatacat ggaatatata
     2521 gcattaacgg gtgaggtaac gataagatat ttacaacccg catttattga ggagaatctt
     2581 tatatacgag gctggtttgt gaaaaagaat aaacgggtta ttgaaaacag agcagaaatt
     2641 gaaaatgaga tgggaaagat tgtggcacga gccaaagcca aatatattga agtagaagag
     2701 ttaccccagc cggaataaaa taaattgcat tcatagcagc cgaagtggtg gaattggtag
     2761 acgcagcgga ctcaaaatcc gccgggggca actccatgag ggttcgagtc cctccttcgg
     2821 catatatact atacttatgg aaaaagttgc gggagtgatt actataattt atttcaagct
     2881 caatatataa atgggcagac aactgattca tcgccattat aaactctttc taaagcctct
     2941 aacatcttca ttgtttgtaa aggatcattt aatgccgttg tccaattcct cacaaaaagc
     3001 aactcgaacg tagatccgaa gatgctacta agacgtgtaa cttatatcaa ctgttatgcg
     3061 attaagatcc ttcaaattat tgatgaaagc atcattcgga atattaatat ttatatatgc
     3121 actttcaggt ttcaattaca atatgttaat gttgttgaaa ataattacta agttatacta
     3181 ttttatttgg tgttgctttt cattcttgaa attagcttta ttattcaatt tttataaata
     3241 ttactttatc gaaaagaacc tgaaatctaa aattgttaaa tattttgtgc tttattttca
     3301 cacaaccaat aattttatgg tatattatta atatgtgata ctatgtatcg catattaatc
     3361 aattttttta ggggtatgag gatattctat gccaaatcag atgccagaaa atttaaaaaa
     3421 tataaaagat atattggatt tagacgaaga gcttcataaa gtatcgcctg aagctgaaac
     3481 tgttggtgaa aaatcaggcg tgagagccaa aaaaggtgat atcaattttc aactgaaacc
     3541 tgaggaatta gaaagttatt tgaataaata tatagtgggg caggaagaag cagtagagat
     3601 tatcgctact aaaatatgta ctcattttaa tcgcatgacg ctggaacagg aattacctga
     3661 tgatgagaag attgttggca atattaaaag taatatgctt ttaattgggc caacaggagt
     3721 tggaaaaaca tatattatca agctcattgc gaaaaagata ggtgtaccat ttgtaaaagc
     3781 tgatgcaacc aagtttagtg aaacaggata tgttggtgga gatgtagaag atctggtcag
     3841 agagctggtt cacgaggcag atggtgatgt tgcaaaggca gaatatggga ttatttatct
     3901 ggatgaaata gataagattg cttcaagtgg aaatgtctac ggacctgatg tatcacgagc
     3961 aggtgttcaa cgtaatttgt taaaacttat ggaagaagcc gatgttgatc ttaaaacacc
     4021 acatgactta gcctcgcagg ttgaagctgc catggaagct cagcgtaccg gaaaagtaac
     4081 acgtaaaaag ataaatacca aaaatatatt attcatagta tctggtgcat ttagcggttt
     4141 ggaggatatc attaacaaac gcttaaacaa gcaggctata ggatttgata aatcgcagat
     4201 acgcagagaa gatcgtcaaa gcgttttgaa gcaggtaaag accgaggatt taattgaatt
     4261 tggatttgaa tcagaattca ttgggcgttt gcctgtaacg gtagtactga atgatcttga
     4321 tgaagaagga ttatataaga tacttaaaaa taaatatagt actgttgttc ttgggaagaa
     4381 gcttgatttc agggcatatg gcatagatct ggaatttatg gatgaagcat tgaagatgct
     4441 tgcagccaag gcatataaag aacgaacggg cgcccgggga ttattaagtg tatttgaaaa
     4501 aacacttatt aaatttgaaa aaacattgcc atcaactgat attaaaaagt tggttgttaa
     4561 ttcagtagtt gtggataatc ctgaaagtgt attaaaaaac atattgttga gtgataatat
     4621 tagaaaattt cagaaagagt ttttattaaa acatggcgtt gttatggaat ttgacggaga
     4681 ggctttacag ataatacagg aaaaagcacg tgaagcaaaa atgagcatca agcaattttg
     4741 tgatgattta tttcatgatt atccctatgg tataaaactt atgaaccttg ataggtttgt
     4801 tataactaaa ggtgctgttg aaaatcctca aagttatatt gatgagnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnatga taaaaagaga aaaacaaata aaaaagccac
     4981 tctttttgat aaggttttta cagttattat tcaatataaa gagacatctc ttttcagatg
     5041 gatatacaat catcctggta tagccatgag tgtggttgca ttatggggca ttataatcat
     5101 tgtcatgttt atgtggtttg taatttcgct tgctacccct tgatatagtt atgctctttt
     5161 gcaactaatt taacctgtgc actcaaatta gatatgtatg cattccgttc ttgatagatc
     5221 agcccttttt tatcatattc ctcaaaagta atcagttcaa ttgctttttc aatatcgcca
     5281 ttcacccggt caagatattg taataaaagt tctttatatt gtttggtaga tgtaatggat
     5341 aattccaaaa actttgttac atcatcatcg gtgtatacaa aaccatgtgc catacaaagg
     5401 gtttgcggtt taagggataa cattttttct aaagatttac agtaatcgtc atacgaggat
     5461 aaaaattcaa cctgcacaat ttctccatta atgccctggg gaaccccgac agcttcgcca
     5521 gcacaaagca ttccaatctc agggaagaaa tatgcaaggg aatcgcgtgt atgaccggga
     5581 acttcatata caacgcaact aatatcccct aaatcaatga aatctccttc tttaacaggg
     5641 caagttatat caaagtatga aagcgatacg tcttcattgt tgacaatatc tgcaaaaaga
     5701 ggtttttgta tatcgctgag atgcgtcatg aaatcaataa ctgtttgttt agaaagcaga
     5761 tcctgtatgc gtttggcacc taccactgat acattgggga ttttcttcag taaatatggt
     5821 atagctccca gatggtcata gtgtgagtga gtaatagcaa cataatcgag ggcaattggt
     5881 tcaccaaata gtgatagcag atcgctgtaa tattttagtg ctaaaagatt aatccctgca
     5941 tctatcatta tacttttagt atacccctta ataatatagg aggggtagcc aatatggcca
     6001 attgcctgac aaaatgtatt aattgttccc tctgcaagaa gtttcatttt tttatccttt
     6061 ttatagatac tattattgct acaactattg ttgcacaata tgatgataaa aatattttct
     6121 atacagttta gctataataa gctacaaaat gtctatcttt tccactgtaa gaaatttttc
     6181 aataacattt ggtaaaatat ttatgtttaa aattatttgt accatattaa taatttgcat
     6241 aattttgaaa ataaagctac attgtttata gtaagaatgt atacaatatt gttatgaata
     6301 ctaaaaaata tattaacatc acaattttgc tggggatgat tgcttcagga tcattacagg
     6361 tttttggttc agatgtaact atctctaatg tccgtccaaa cctgatgaat attagaacaa
     6421 ccaatggcaa attttatgca tatattgtat tgttgtttaa cgaaaaccct cattttgtaa
     6481 accttatcca ggcagatagt attccagtaa ggttgatgca aacaactggt tttaatattg
     6541 tgcaatacga agcatacctg aaaacattac aggatacttc tttaaaaact ttagcacaac
     6601 aggtattgcg ggtgtacaaa gaacctgata agatatctga taatgaactt gaacagctga
     6661 cacaaaagct ggagaacaga aatataatac tgcgattttc aaaaacaaaa gatggcggtg
     6721 agtataaata tttactggat tattgcattt atgggaaaag aaaaccggta actatcaatc
     6781 atccgctttt taatgtaaag gtaagaattt ttaatataca acctttaatt tattatgatg
     6841 aattttcgac aagtaactcg acgttttatt ttgatatgat ttatataaat cctgatgaag
     6901 tgtacaatga ttacataata tctaaaagaa ttatggataa caaatcatgc gaaaacatgt
     6961 tttttgtagg tgcacatgtt aatgaggata tacgcttttg cataaaaaaa tcattttcca
     7021 gcaaagaaac aatctttgat gagatatgga aaatgtttga gatacatgag ttaacacata
     7081 aaattttgaa taattattat tacttttttg atcaggtgac aggtgaagaa ctggcattaa
     7141 gcagtaccat ttattacaat acctatcttg gccttgcaat actatatagc tatctcgatt
     7201 ataatactat taatcctcat cgcatcgcag cttctaatta tattaaattt ttgtctttat
     7261 acacagctaa cggtgaatat attgaaaaac catcccttat aaaaaattta cccattgagg
     7321 atattaaaaa aattgccagg ctacattttg aaaccacaat aaaaaattta aaataacatc
     7381 tcaattgcgt aatcgtatag gattttgatg gatcatgcca tcaaggctga aacctttttt
     7441 tccggtgtag gcatttgtaa attcgattac agcattgaat tgagaaggtg aaatcatttc
     7501 tttgtggttt aatagttctt caaattgttt ccagagtgtg acagtttttt ctttcacaat
     7561 tctgaaaagg tcatcaactg attcgtaatg taagcccact tcaccgccgg gattaaacca
     7621 tcgatcacga tgcagattta aaatatgacg gttaatcctt cgtgcatcag gataccgtac
     7681 caggaaatca ctgtaagttc ttttttcaag taagcgaaga atcttaataa gatgcgcatt
     7741 tcttgttttt tttaatgaat aaactggttt tacacattgt aatgcgatat cataaccatc
     7801 atgttttaac catgaaataa tggatggtgt aagagtgagg gacggtttta tggtggcgat
     7861 agcattagta aaaatatctt tggatgcttt gtacagtata gtgttttttt gtgggatcat
     7921 acggtctaaa ttataatcaa aaggttctga attatagaaa taagccatat attgatcaat
     7981 agtatattca aagagaagat gttgattgcg atagtacgac accctgttat ctttatatgt
     8041 tgggaatcca gcccagtaaa atacaaaagg atgatacatc gcatcagtaa cccagtgtga
     8101 aatatatccg taaaaaaaac ctttttgata gtatacccat tcatttggtt catggttatt
     8161 atttaaaagg gtgttaaaca tggcaaaaat aacagataga ccatcatccg aatgcaaaaa
     8221 atgttttata ctgttgccaa aaaaaatgtc gcgcttacct ggtaggtaat caaaaagatt
     8281 tggtattagc aatcctgcca gagcagatga catgaaaaga ggatgggaaa actgtgcatc
     8341 aagcaaatgc gtactatgat caggatgttt tagcttcttt aatgatgtaa ctgattcaaa
     8401 aaagatacgt tgatgcgtta ttaatccagg cataatgaat atgattttaa aatttattaa
     8461 gggggaattg tatattcccc cctttttgtt ttatcaccgt ttatgacact gtgtgcaaag
     8521 tgtaggtgcc ttaccgcctt tagttctgtg acaacccagg catttggtat gaatagcatt
     8581 gtaaccctta ttttgtgttt gccagtgaca accgttatca ccgcattttt ttgctgtaga
     8641 accagtatga tgacatattt tgcaatctgt aattcccctg gctttatgga tttgatgatt
     8701 aaaggtaaca gtaccctgtt tagggacagt agtagtcttt ttaaccttta caatgctttg
     8761 agcaagtata aaagcaggta tcaatagaat aaggcagctt atcaacgccg catttttttt
     8821 attcatagta cctcactata ataatgtata tcatttgcgg ataaacaggt cattctccca
     8881 tgacccatca aaggtggtac catcgttaag aattagcttt cctttcccat ggcgcttatc
     8941 atctttccac tcaccttcat agcgggttcc gtcagcccat gtataaatgc caaaaccatt
     9001 tcgtttgcca tctacccagt tgcctttgta gctatcgcca ttcttccagt aatattcacc
     9061 ctgtccatta cgcatattat tgctgtaatt acctctatac cggtcaccgt tcgtgaaatt
     9121 gtattcaccc ataccttcac gaatactatt attaaagcta ccgttatagg tgccattatc
     9181 atgagtagat gttcctattc catttttaca gtcaccttct ttacaatttt ttggtgacga
     9241 aacacaactt atagcaaaaa taaaaataaa aataggtaaa aaatatctta caggtttcat
     9301 gaaacacctc cttacctcaa gaattatgat aatcttttta taattaaggc attatagaca
     9361 atgaatatat aattagcaat tagttttttt attttttgtg ctttgcgata gttctatcgg
     9421 gataaaagct gctatgtagc aataaagttt atattttttt tataagcatt gtatgcatag
     9481 taattagatt aattaaaata ttttttttga aagatataca tttttgtgat aacttatttt
     9541 aagactgcat tggtgatgac cttgccatgc gtctttatat aaaattccgt accacaagta
     9601 cggaattttt atttggtaac attgtttatc cactctgcat aatcattgga tactgcaact
     9661 accggcaatg caataatttc agggatttca tacgggtgca gtgctttaat gcttaattca
     9721 acttcatggt aaagatcttt ttttgttttg attataagca ataattctga atcgttgcat
     9781 atctcgtttt tccatgaata cactgacatg agcccgctaa ctatgttaac acatgctgct
     9841 aatttgttat tgaccagatg atctgcaata gcaagtgcaa catcattgtc agggacggta
     9901 caatacatta taattatatc catattattg tccttttact tttcaagtaa ccgttttttc
     9961 cgtcgtagtt ctgcttcttt attccgtctt ttttcctcat cagtttcagg aatgagtgct
    10021 ggaacttttt caggttttcc tttttcatca agtgctacat aggtaagata tgctgaggca
    10081 gtgtgccgca ctacgccggt tataaagttt tcagcttcaa ctcgaacgcc cacctccatt
    10141 gatgttgtac caacaaagtt aacacttgcc ttaagacgca gtaaatcacc aacataaaca
    10201 gggctatgaa aatccaatct gtcaattgag gcggtaacta ccagacaccc ggaatgtcgg
    10261 gaggcaacaa tcccaccggt attatcaatg agcttcatta tagtgccacc atggacattg
    10321 ccggctaaat tagcatcctg atgtgtcatc tgttgaacta ttgttatggt actatcgtga
    10381 acaaattttg tatccatacc tttatcccta catgaaagat gttatgcttg atatagtatc
    10441 tttataatta ttgtagatgt tcattataca ttttaaaatg taaaaatatt gaataagcaa
    10501 atgaaaaatt gcaaccaaaa aatatataat actgtcttta tagaatagta ctatataaac
    10561 aaaatatggg gatgttatac tatgccggta aacaaaacag aacttgtttc attttattca
    10621 catgcaatag ctgcattggc ttcattcatt ggattattta tacttataga agctgcacag
    10681 tcttatattg taaagatact atcagcagta tacgctattt ctgttattgc attattttca
    10741 gcaagctcgc tttatcatgg attaaaacgc caggataatg aagaatcact atggcgtaaa
    10801 cttgatcata tggcaatatt tattatgatt gcgggaacat acacgccggt atgctacctg
    10861 tatcttcctg ctacaaaagc atttgccata ctgtttttcc agtggttgtt ggtagcatca
    10921 ggtatactat ttaaattata ctttttcaat gctccaagaa ttatttatac cacaatctat
    10981 ttgataatgg gctggatggc aattgttgtg attaaggata ttttgcatat aatggatagc
    11041 acatcgctat ggtacctatt tgcaggtggc attgcattca cagcaggggc agttttttat
    11101 attatgcaat ggcctaagaa gatacagggt tatgttggat ttcatgaatt ttttcatttt
    11161 tttatcatag cgggagcttt gtgccactat ctcatggttt accgaggagt aaaatatatg
    11221 tgatcctatt atctatcctg tgcccgcatt caaagacgcc ataattccct gtatggcata
    11281 ttttttctta aaatcccata gggatgacat ttt
//
LOCUS       DAOOJH010000078        11114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5341_length_11114_cov_10.226241, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000078 DAOOJH010000000
VERSION     DAOOJH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_11114_cov_10.226241"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PLJ75_09615"
     CDS             complement(<1..2351)
                     /locus_tag="PLJ75_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05509.1"
                     /translation="MHIQEFNYSNTAQRRILLLSLFFSIVTLSSYAIMSTLFLTSIVL
                     YQAVWKYILTLSLSGIATGIIIGTIIHKKITVSRMLLILLQIVISCLAIAPCIASYFD
                     NGMETIYLAIAQKFSLLFYCLIFLLFTLEGIKSAYFLKTSIGDYIDNKNPALFAISVA
                     LFAIVFSLYLSLLLYSHMEYLWIYFFFPLLSLILIIFLNLPFCPPTIISRTAITKPDD
                     ISTRDDVLFTYSNISIISIFTTLILITIYSQQGIFIATTMYTISTIIGGFLIGMFLST
                     LIKKYNTVFIYGEMLYPLLFLAFIIISHTTSHSLYSSLLAATPFSIFAGITFGTSLKH
                     ILIKNNHSTRYTIILFSFFLIPLPIIIAFIWIELTYFWFFAVTYILSIITIVFPGLYL
                     LQNFKRSVPSIIYCIVSLLFIPAIIVTHMYFKLPLSDNLVNAYSRGYGDVVSSNFNSS
                     FITKPGVAYINDCVACYITDATARDLKRSIAFISLFANNHNTIIIDGYHKFFTNSLFH
                     LLNSPIVLDYIPDRIVDYQKPAISDVHFATIHTSIIQGLKQKKGLIVDIPNTFDHYMY
                     PFRFSDAYIAIIKEHVDSYYFTIIDITNIAPNYLYSLTNALKKYFLHTVVFISGNHLT
                     IGATNNSNGFILGASDIQNLENLIQTTEVANLFVNAEHALSYMVSNTIELIMPRNGVL
                     TTFTLPITKPAQQLFINPFVEKVITTNDAVVQIIDNQYYYANTIGLRMLYAKQINQLI
                     KQSEYYGLQQHYESEMQTLMQLRRIAEYTPALRQYITNIISFK"
     gene            2716..3036
                     /locus_tag="PLJ75_09620"
     CDS             2716..3036
                     /locus_tag="PLJ75_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05510.1"
                     /translation="MQSKNAFEYIPEYNLVVLFVLILCTAGIYYFWWIARVSRIFYDN
                     PVTNILLIILTAGIWLVYITIKYMQKSEMLNDRQMPWYMILFLPIGFLIIQHNINERY
                     FTSQ"
     gene            3054..4799
                     /locus_tag="PLJ75_09625"
     CDS             3054..4799
                     /locus_tag="PLJ75_09625"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HPD05511.1"
                     /translation="MIVRCSECNSAYQVDDEKVQNKRFAFTCPRCKAHVIVDNRAEGY
                     DASFDDMMLAQDTGKPHTINEPVDTFIDEESSAKRFSEDTIAQTDEELLADIEKLEDF
                     EKTSKIKKETDAFAPIESESIDLSDFSAEELAGATDDLNIQQEAPESDIVLEGDIKSD
                     EIFSKEKDLDESITIDLDTLDIPIEESAVIEQAEMPLTEPDILEKVELPEEEIGFDDI
                     LVSEEPARKPKTTISPMRKEKEKEKLDDITLDLDSLDIQLDEHAETLPGEQIIDEDEK
                     LTLEDAGITIDELIEEQKKETIEEEEELKLSLDEIEPGLTADDLHKELTESELETIIA
                     SEENFDTYTSKDEIELPEVDLDKYEMGAGEVEQQKAGYHHAEKEDFDSVPAGFVNLTI
                     DYSLSYSRANAILRLLGVYLLSLLPHIIVCVVYSIVSSIVGLFNWIIVLFTGQFIDDF
                     ADIQQNTLRYWLSIVACAAQITEDRPVYAGYPLVEHSLQLDIVYPSSPSRLLALLRIS
                     LIGIIIALLPHLLLYVILTMGVLLLVPIGLISIVAIKRWPTLLFDFMYRYMRYAARIW
                     AYAAGLVDKYPSFIF"
     gene            4863..>6818
                     /locus_tag="PLJ75_09630"
     CDS             4863..>6818
                     /locus_tag="PLJ75_09630"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HPD05512.1"
                     /translation="MDINEIIHTVFTDGVLLNYIPDYEYRAIQEQMADAIITAWYNEK
                     HCIIEAGTGTGKTLGYLLPAALFAMHEDVKVAIVTETKALQQQLMLNDLPIIANAIRD
                     ITGTSLTYALCLGSNNYPCYRRLQNAIDTGSFDKSKEKEIYHVHDVVTKQPALTRFDI
                     MAGESLWHTICRESQLCPIHTCKYKNRCSYILAKKNWQEANILVMNHYLFFSNIAASK
                     SLVPDFKVVVFDEAHSIERIASNQLGFAINTEQLKENLEHIIRRRKGGALLERVLTYD
                     MISEAMEVFDNALRQVQLSKKILNDIVKNLQTLRLRNPLAECLPLLKALEEVLRLFEK
                     AEPETMDEDLRLEYEYHKASIMDIWHDLGMAVFMDEESYVYWLEKEKNSQVNIFICGQ
                     PLDVASIIHKEVLDYYDHCCFVSGTLSVNNSFEYFKQSIGCDNADTLILSSPFNFTTQ
                     SVLFIDTNDIDPNSEEFIDASVARLTFIVNMVKGRCLVLFTSYTMLIGVYEKMNDTIP
                     YKIFSQHRVNASTILHEYITCSNGVLFGTHSFWQGIDLPGDLVRAVIIMKLPFEAPDS
                     PVVQAKIEKIQKEGGNPFMKYQLPYAVLLLKQGFGRLIRKSTDKGVVAILDSRIVTKA
                     YGKIFLASLPEAKKVYTLEELEKAYTAM"
     assembly_gap    6819..6918
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6919..7205)
                     /locus_tag="PLJ75_09635"
     CDS             complement(<6919..7205)
                     /locus_tag="PLJ75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05513.1"
                     /translation="MEKTHRVSSTIMSNIPVLILEGDITSEADADIMNEYRQLKEKQA
                     PFYCIIDFTKTKYINSAGIAILINIIQDFGDFGGKVVFTGLSPHFNKVMDIV"
     gene            complement(7279..9489)
                     /locus_tag="PLJ75_09640"
     CDS             complement(7279..9489)
                     /locus_tag="PLJ75_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPD05514.1"
                     /translation="MDLLQKLNEEQYNAVTYSDGPLLILAGAGSGKTRVITYRIAHLI
                     KVAGIPPYAIVAVTFTNKAAEEMRNRIKGLIGPIGESVMIKTFHALAVYILRRHGDAI
                     GIAPNFTIYDSRDQESLIKDILHDIHIDPKAVKPSMIAEKISSIKESSHYIQGGDITL
                     LLPKYHSFNFQEIFTSYQQKLKQSNALDFSDLLIYTVQLLRNSQVLTTLQSRWRYFMV
                     DEYQDTNFAQYLIAKYLASQTKNICVVGDDDQSIYSWRGADIRNILNFEKDYPDAKVI
                     TLHKNYRSTAPILQAASYVIKNNYRRKDKKLTSHRGDGEPITVCTANNEYGEAEYVIN
                     TIVSLKHTEQYSNRDFAIFYRTNAQSRIFEEHCRRENLPYIVVGSVKFYERKEIKDIL
                     AYMRFMANPDDTVSLLRIINTPSRGIGASTIEKLRDAAYLHTTSEWDIIANNQNYSYK
                     LSPALHSFKELMQKLIDKVKDIPSHYRLSEICISILTDTQYKSTLESENSIEARGRLE
                     NIDAFVNSIVEYEHLHPEATLFEFLQDISLLSPADETNATAPEGNNAITLMTVHNAKG
                     LEFPVVFLTGMEEGLFPHANAIDSDEGIEEERRLCYVGITRAKDRLVITSAELRRSWG
                     TTQFKEASRFISELPPALCQFQHYSSSYNNWDMPKTNNNNYNYRANNSQTATALHNND
                     SHYTVNQRVKHPSFGVGKITAIQGKGDNVKLTIQFLNGFTKTFLEKYTPLEPLS"
     gene            9691..10218
                     /locus_tag="PLJ75_09645"
     CDS             9691..10218
                     /locus_tag="PLJ75_09645"
                     /inference="COORDINATES: protein motif:HMM:NF016611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05515.1"
                     /translation="MRNIYTTVLLMVSMALASGAIARNNDEAASQYNGAGLYCLESGN
                     YQKASEYFIDAIQLNPANKFYYNNCAVAWMNMGRYEDAMDMLQIALAIDPHYVKALTN
                     MAIVCFHLLKFSRAYYYYSQARTADSIYTKERFQMAKVIAGVKRVYARHPDNNDLQQI
                     LNQLEILQQRKKDLP"
     gene            complement(10221..10670)
                     /gene="dtd"
                     /locus_tag="PLJ75_09650"
     CDS             complement(10221..10670)
                     /gene="dtd"
                     /locus_tag="PLJ75_09650"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPD05516.1"
                     /translation="MRAVIQRVLQASVEVDGNSIASINKGLAVLAGFAPSDTTKDLEY
                     ICQKIIGIRIFDDASFFMNLSLSDVHGGLLLIPQFTLYGDARKGRRPSFSDAMKPETA
                     SEYFDSFVAMCKAAHPHVVTGKFKAHMKVILVNDGPVTILLDSSKLF"
     gene            complement(10674..10988)
                     /gene="trxA"
                     /locus_tag="PLJ75_09655"
     CDS             complement(10674..10988)
                     /gene="trxA"
                     /locus_tag="PLJ75_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017660073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPD05517.1"
                     /translation="MGTIFEVNDTNFNAEVLECAGKVLVDFWAPWCGPCRMQTPILEK
                     LAQDPETKTKIVKVNTDNNPETAQRYGISSIPTLILFDNGKEIERMIGVQPENTLKKK
                     LS"
BASE COUNT         3454 a         1799 c         2255 g         3506 t
ORIGIN      
        1 tctttaaagc taatgatgtt tgttatatac tgccgcaggg caggggtata ttcagcaatc
       61 ctgcgtaatt gcatcaatgt ctgcatctct gattcatagt gttgctgtaa tccatagtat
      121 tcggattgtt taatgagctg attgatttgt tttgcataga gcatgcgcag tccaatagtg
      181 ttagcataat agtactggtt atcaatgatc tgtactacgg cgtcattggt agtaataacc
      241 ttttcaacaa agggattgat gaatagttgt tgtgctggtt tggtaatggg tagcgtaaat
      301 gttgtaagga cgccattgcg cggcatgatt aattcaatgg tatttgatac catgtatgat
      361 aatgcgtgct cagcattaac aaaaagatta gccacctcag ttgtttgtat caggttttct
      421 aaattttgaa tatcagatgc accaagtata aatccgttgc tattattggt tgctcctatt
      481 gtcaggtgat tgccagatat aaacacaaca gtatggagga aatatttttt taaggcattt
      541 gtcaggctat agagatagtt tggtgcaata tttgttatgt ctattattgt aaagtagtag
      601 ctatcaacat gttcttttat gattgcaatg taggcatcag aaaagcggaa aggatacata
      661 taatgatcaa aggtattggg tatatcaact atcaaccctt ttttttgttt taatccctga
      721 ataatggatg tgtgtatggt tgcaaaatgt acatcactta ttgctggttt ttgataatct
      781 actattctgt cggggatata atccagcact atgggtgaat tcaggagatg gaaaaggctg
      841 ttagtaaaaa atttgtgata tccatcaatg ataatagtat tatgattgtt tgcaaacagt
      901 gatataaatg caatggatct tttcaaatca cgtgcggttg catcggttat atagcaagct
      961 acacaatcat taatataagc tactcccggt ttggttataa aagaagagtt gaaatttgat
     1021 gaaaccacat ctccgtatcc tctgctgtat gcattaacaa gattatcgct caatggaagt
     1081 ttaaaataca tatgggtaac aatgatagca ggtatgaata ataaactgac aatacaatag
     1141 attatggatg gaaccgagcg tttgaaattt tgcaataggt ataaaccggg aaatactatg
     1201 gtaataattg acagtatata ggttacagca aaaaaccaga agtacgtaag ttctatccag
     1261 ataaatgcaa taattattgg caggggtata aggaaaaatg aaaataaaat gatggtatac
     1321 cgtgttgagt gattgttttt tattaatata tgttttaacg atgtaccaaa tgtaattcct
     1381 gcaaatatgc tgaatggggt tgctgctaaa agactgctat ataatgaatg agatgttgta
     1441 tgacttataa taatgaatgc taaaaacagc aatggatata acatttcgcc ataaataaag
     1501 acggtattat atttttttat caatgtagat agaaacattc caattaaaaa gccacctata
     1561 atagtgctga ttgtatacat tgttgtagct ataaaaatcc cttgctggga ataaattgtt
     1621 atcaatatca aagttgtaaa tatactgata atagaaatat tgctataggt aaaaagaaca
     1681 tcatcgcgag ttgaaatatc atctggtttg gttatggctg tgcgcgatat aatggtaggt
     1741 gggcaaaaag gcaggttgag aaaaatgatt aaaattaatg atagcaaagg aaaaaagaaa
     1801 taaatccaga gatattccat atgactgtat agtaacagtg aaagatacaa tgaaaaaact
     1861 atggcaaaca atgccacact gattgcaaaa agggcaggat ttttattatc aatataatcg
     1921 ccaatagatg ttttaagaaa gtaagcggat tttatacctt ccagtgtaaa aagaagaaag
     1981 attaagcagt agaacaatag ggaaaatttt tgtgcaatgg ctaaatatat agtttccatg
     2041 ccattatcaa agtaagaagc aatacaagga gctatcgcca aacatgatat tactatttgt
     2101 aatagtatta aaagcatcct gctaacagta atttttttat gtatgatagt accaatgatg
     2161 atacccgttg caatgcctga aagggaaaga gtaaggatgt atttccacac cgcttgatag
     2221 agtactattg atgtaagaaa taatgtactc attattgcat acgatgaaag tgtcactatt
     2281 gaaaagaata acgataacaa taaaatcctg cgttgagctg tatttgaata attaaattcc
     2341 tgaatatgca tggtatcgat tacattccct tcattattta ttaacgattc atgaaagaca
     2401 attggatttt aagacctatt atactgttat gataaaaatg acaagaattt ttctatattt
     2461 tagtaagtgt gaaggtgaga ttaggaggaa atagctggta tgatgtgtat cgttaatttt
     2521 tgttgctata ccataggtag ttattgtagg gtaaacatta atttaatgta tgaatattga
     2581 ataattatta agattttttc actattttac cgtaaattat atatagatac ttttagactt
     2641 tttttgttgc atgattttag ttattttact ataactgtat tctctatgac agtaaaagtt
     2701 aaaaagggag cttatatgca gtcaaaaaat gcttttgaat atatccctga atataatctg
     2761 gttgtgctct ttgttttaat actgtgtacg gcagggattt attatttctg gtggattgcc
     2821 agggtgagca ggatatttta tgacaaccct gtaacaaaca ttttactgat tattttaaca
     2881 gcagggatat ggcttgtcta tattaccata aaatatatgc agaagtctga gatgcttaat
     2941 gacaggcaga tgccatggta tatgattttg tttttaccta tagggttttt gattattcag
     3001 cataatatta atgagcgata ctttaccagt caataaacca tcaggagcaa tcaatgattg
     3061 tacgatgcag cgaatgcaat tcagcctatc aggttgatga tgaaaaggtg caaaataaac
     3121 ggtttgcatt tacgtgcccc agatgcaagg ctcatgttat tgttgataat cgtgcagagg
     3181 gttatgatgc aagctttgat gatatgatgc tggctcaaga tactggcaaa ccacatacca
     3241 taaatgaacc tgttgataca ttcattgacg aagagagtag tgctaaaaga ttttctgaag
     3301 atacaattgc tcaaactgat gaggagttgc tggctgatat agaaaaatta gaagattttg
     3361 aaaaaacttc aaaaataaaa aaggagacag atgcatttgc tcccatagaa agtgaaagca
     3421 ttgatttgtc tgatttttct gctgaggaat tagcaggtgc tactgatgat ttgaatatcc
     3481 agcaggaggc accggagtcg gatatagtgc ttgaaggtga catcaaatca gatgagatct
     3541 tttctaaaga aaaagacctt gatgaaagca ttacaattga tctggacacc cttgatatac
     3601 caatcgaaga atctgctgta attgaacagg ctgaaatgcc tttgacggaa ccagatattc
     3661 ttgaaaaagt agagttgccc gaggaagaga ttggatttga tgatatactt gtttcagaag
     3721 agcctgctcg taaaccaaaa acaaccattt ctccgatgag aaaagaaaag gaaaaagaaa
     3781 agcttgatga tattactctt gatctggatt cacttgatat tcagcttgat gaacatgcgg
     3841 aaaccttgcc cggggaacag atcattgatg aagatgaaaa attaacgctt gaagatgcag
     3901 gcatcactat agatgaatta attgaagaac aaaaaaagga aaccattgaa gaggaagagg
     3961 aactaaaact ttcgcttgat gagatagaac caggattaac tgccgatgat ctccataaag
     4021 aacttaccga atccgagctt gaaacgataa tagccagtga agaaaatttt gatacttata
     4081 ccagcaaaga tgaaattgaa ttgcctgaag ttgatcttga taaatatgag atgggagctg
     4141 gtgaggttga acagcaaaaa gctggatatc accatgcaga aaaagaagac tttgattccg
     4201 tacctgctgg ttttgtaaat ttaactattg actactcatt gtcgtattca agggcaaatg
     4261 caattctaag acttcttggt gtttatcttt tgtcattact acctcatatc attgtatgtg
     4321 tagtgtatag cattgtttca tcaatagtag gattattcaa ttggattata gtgctgttta
     4381 ccggacaatt tattgatgat tttgccgata tacagcaaaa cacattgcga tattggttat
     4441 ctattgtagc ctgtgctgca cagataaccg aagaccgtcc ggtatatgca ggatatccat
     4501 tggttgaaca tagcctgcag cttgatatag tgtacccttc ctcaccatca cggcttctgg
     4561 ctttattgcg catttcattg attggcatta tcatagcatt gttgcctcat ctgctgctgt
     4621 atgttatttt aacaatggga gttttattac ttgttcctat aggattaata tcaattgtgg
     4681 caataaagcg ctggccaaca ctattgtttg atttcatgta tcggtacatg cggtatgctg
     4741 cgcggatatg ggcatatgca gccggtctgg ttgataaata tccatcgttc attttttgat
     4801 agacataaaa tatcccggtt atatacatta cctcaattaa tcaaatacat acatagttgt
     4861 ttatggacat taatgaaatt atacatactg tatttactga cggcgtgctt ttgaactata
     4921 ttcctgatta tgagtaccgc gcaattcagg agcagatggc tgatgcaata atcactgcat
     4981 ggtataatga aaagcattgt atcattgagg caggtacggg tacaggtaaa acgttagggt
     5041 atcttttacc tgctgctctt tttgcaatgc atgaagacgt taaagtggcg atagttaccg
     5101 aaacaaaagc tttgcagcag caattaatgc ttaatgattt acccatcatt gccaatgcca
     5161 tacgcgatat aacaggtact tctcttacct atgccctgtg tttaggcagc aacaattatc
     5221 cctgctatcg ccgtttgcag aatgccatcg atacgggaag ttttgacaaa agtaaggaaa
     5281 aagagatata ccatgtgcat gacgtagtaa caaagcaacc tgcgctgacg cggtttgata
     5341 ttatggcagg tgaatccttg tggcatacta tctgtcgaga atcacagcta tgccctatcc
     5401 atacatgtaa atataaaaat agatgttcat atattttagc taaaaaaaac tggcaggaag
     5461 caaacatcct tgttatgaat cattatcttt ttttctccaa tattgctgct tcaaagtcat
     5521 tggtgcctga ttttaaggtt gtggtgtttg atgaagcgca ttccattgaa cggattgctt
     5581 ctaatcaact tggatttgca attaatactg agcagctcaa agaaaatttg gagcacatta
     5641 tcagacgcag aaaaggaggc gcattacttg aaagggtttt aacgtatgat atgatcagtg
     5701 aagcaatgga agtttttgac aatgcactgc gtcaggttca gttatctaaa aaaatactca
     5761 atgacattgt aaaaaattta caaacactgc gattgcggaa cccccttgcg gaatgtcttc
     5821 ccttgctcaa agctcttgag gaggttttac ggttatttga aaaagctgaa ccagaaacaa
     5881 tggatgagga tttacgatta gaatatgaat atcacaaagc ttcgataatg gacatatggc
     5941 atgatctggg gatggctgta tttatggatg aagaaagtta tgtgtactgg cttgaaaaag
     6001 aaaaaaattc acaggttaat attttcattt gtgggcagcc gctggatgtt gcctcgataa
     6061 tacataaaga ggttttggat tattatgacc attgttgttt tgtttcggga acgttgagcg
     6121 tcaacaattc atttgaatat tttaaacaaa gtattggatg tgataatgca gataccttaa
     6181 ttttatcgtc gccctttaat tttacgacac agtcggttct gtttattgat accaatgaca
     6241 tagatccaaa cagtgaagaa tttattgatg cttctgtggc aagacttacg tttattgtca
     6301 atatggtaaa ggggcgctgt cttgtgctat ttacatcata taccatgctt ataggtgtat
     6361 atgaaaaaat gaatgatacg ataccataca agattttttc acaacatcgt gtcaatgcgt
     6421 ctacgatatt acatgagtat atcacatgca gtaatggggt tctttttggg acacattcat
     6481 tctggcaggg gattgatttg ccgggtgatc tggtgcgtgc ggttatcatc atgaagttac
     6541 catttgaggc tcctgatagt cctgttgtgc aggcaaagat agagaagata caaaaagagg
     6601 ggggcaatcc tttcatgaag tatcaactgc cgtatgcggt gctgttgtta aaacagggct
     6661 ttggcaggct gattcgcaaa tcaaccgata aaggagttgt tgctattctt gattcgcgaa
     6721 ttgtaacaaa ggcgtatgga aagatatttt tagcttcgct tcctgaagca aaaaaggtgt
     6781 acacgctgga agaattagag aaagcatata ctgctatgnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnac aatatccata accttgttga aatgtggtga taatccggta
     6961 aatactacct taccgccaaa gtcaccaaaa tcctgtataa tatttattaa gattgcgatt
     7021 ccggcagaat tgatgtactt tgttttggta aaatcaataa tacaatagaa cggtgcctgt
     7081 ttttctttca attgccggta ttcattcata atatcagcat cagcctcaga ggttatatct
     7141 ccttctagaa tcaatacagg gatattgctc attattgtag acgatacccg atgagttttt
     7201 tccataatcc acacctctga aagtattctt ttgtttataa ttcatgtatt cttaagcata
     7261 aattgtcaag aaaaaaaatc atgacaatgg ttcaaggggg gtatactttt ccagaaatgt
     7321 tttggtaaaa ccattgagaa actgtatggt cagtttaaca ttatcaccct ttccctgtat
     7381 agcagtaatt tttcctacac caaacgaagg gtgcttaacc ctctggttta ctgtatagtg
     7441 ggagtcgtta ttgtgtaagg ctgtggcagt ttgactatta ttagcgcgat agttatagtt
     7501 attattattt gtttttggca tatcccaatt attgtatgat gagctataat gttgaaactg
     7561 gcataatgct ggtgggagct cgcttataaa acgcgatgct tctttaaact gtgttgttcc
     7621 ccagcttctc cgcagttcgg cgctggttat aaccaggcgg tcttttgccc gtgttatgcc
     7681 aacatagcac aaacgtcgct cttcttctat cccttcatcg ctgtcaattg cattggcgtg
     7741 agggaacagc ccctcttcca tccctgttaa aaaaactaca ggaaattcca atcccttggc
     7801 gttgtgaaca gtcataagag taatggcgtt gtttccctct ggagcggttg cattggtttc
     7861 gtcagcaggt gaaagcagtg aaatatcctg taaaaattca aacagtgttg cttcaggatg
     7921 gaggtgctca tattcaacaa tgctattaac aaatgcatcg atattttcaa gccttccccg
     7981 tgcttcaatt gaattttcac tttcaagcgt acttttatac tgcgtatcgg taaggatgct
     8041 tatgcatatt tccgaaagcc tgtaatgaga cgggatatcc ttaaccttat caattaattt
     8101 ctgcataagt tctttaaaag aatggagtgc gggcgatagt ttatatgaat agttctggtt
     8161 gttggcaatg atatcccatt cggaagtggt atggaggtaa gcagcatcac gcaacttttc
     8221 aattgtggat gctccaatac cacgcgatgg tgtattgatg atgcgaagca gtgaaaccgt
     8281 gtcatcaggg tttgccataa accgcatgta tgccaggata tccttaattt cttttcgttc
     8341 gtaaaattta acgctgccga caacaatata gggtaaattt tctcttctgc agtgttcttc
     8401 aaatatccgc gattgtgcgt tggtgcgata aaatattgca aaatccctgt tggagtactg
     8461 ttcagtatgc tttaaactaa caatggtgtt gataacatac tcggcttcgc catattcatt
     8521 attagcggtg catacggtaa taggttcgcc atcaccgcga tgcgaggtaa gctttttgtc
     8581 tttgcgccgg taattatttt taataacgta tgaagctgct tgcaaaatag gggcggttga
     8641 gcgatagttt ttgtggaggg taattacctt tgcatcagga tagtctttct caaagtttaa
     8701 gatattgcgg atgtcagctc cacgccatga gtagattgac tggtcatcgt cgcccaccac
     8761 acatatattt tttgtttgtg atgccaggta tttagcaatg agatactgcg caaagttggt
     8821 atcctgatac tcatcaacca tgaagtagcg ccagcgcgat tgtaatgttg taagcacctg
     8881 gctgttgcgc agcaactgca ctgtatagat taaaaggtca ctgaaatcca gagcattgga
     8941 ttgtttcagt ttttgctgat agctggtaaa tatctcctga aagttgaatg aatgatattt
     9001 tggcaatagc aatgttatat cgccaccctg tatataatgg ctgctttcct ttatgctgga
     9061 aattttttca gcaatcatcg aaggttttac cgcttttggg tctatgtgta tatcatgtaa
     9121 tatatcttta atcagtgatt cctggtcacg gctgtcataa atggtaaaat tgggagcaat
     9181 gcctattgca tcaccatggc gtcgcagtat atacactgct aatgcgtgga aggtcttaat
     9241 cattacactt tcgccaatgg gacctatcag tcctttgatg cggttccgca tctcttcagc
     9301 agctttattg gtgaaggtta ctgcaacaat ggcatagggt ggtatgcctg caacttttat
     9361 taaatgagct atccggtagg taataacccg tgttttgccc gaaccagctc ctgccaggat
     9421 taacagcggg ccatcactat acgtaacggc attatattgt tcttcgttaa gtttttgtag
     9481 taagtccata gagtgtattc aaacggattg tagtatgacg atgacataat taataataat
     9541 aaaaatactg caagataaaa aagaaaaatt ataacctgtg taatgaaatt aatattaata
     9601 attgaaaaaa aaatagtctt gtattattct atattttttc attacatatt ctataaaata
     9661 tacaaccgat ttaaaagcaa agaggatgcg atgaggaata tatataccac agtgttgctg
     9721 atggttagca tggcattagc ttcaggagct atcgcccgga ataatgatga agctgccagc
     9781 cagtacaatg gtgcaggctt atattgttta gaatcaggta attatcagaa agcctctgaa
     9841 tattttattg atgcaataca attaaatcct gccaataaat tttactacaa caattgcgct
     9901 gttgcatgga tgaacatggg aaggtatgag gatgccatgg atatgttgca aatagcgctt
     9961 gctattgatc ctcactatgt aaaagcattg accaatatgg ctattgtttg ttttcacctt
    10021 ttgaagtttt cacgtgccta ttattattat tcacaggcaa ggacagccga tagtatctat
    10081 acaaaagaac gattccagat ggcaaaggta atcgctggtg ttaaaagggt ttatgccaga
    10141 catcccgata acaatgattt acaacagatt ctgaatcagc ttgagatatt acaacaacgt
    10201 aaaaaagatt tgccatagat tcaaaataat tttgagctgt ctaaaagtat ggttaccgga
    10261 ccatcgttaa caagtataac cttcatatga gctttaaatt tgccggttac aacatgggga
    10321 tgtgctgctt tacacattgc tacaaacgaa tcaaaatatt ctgatgctgt ttcaggtttc
    10381 atggcgtcag aaaatgaagg acggcgtcct ttgcgggcat caccgtaaag tgtaaattgg
    10441 gggataagta aaagcccgcc gtgaacatca gataatgaca ggttcataaa gaagctggca
    10501 tcatcaaaaa ttcggatgcc tataattttt tgacagatat attctaaatc ttttgttgtg
    10561 tctgatggtg caaaccctgc aagtactgcc aaacctttat tgatggatgc tatgctatta
    10621 ccatcaactt ccacggaagc ctgtaaaaca cgctgtataa cagcacgcat tgtttatgaa
    10681 agttttttct ttaaagtgtt ttcaggctgt acgccaatca tgcgctctat ctctttgcca
    10741 ttatcaaaaa gtataagtgt tgggatagaa gatataccat accgttgggc tgtttcagga
    10801 ttgttatcgg tatttacttt tacaatcttt gtttttgttt cgggatcctg cgctaatttt
    10861 tccagtattg gcgtttgcat gcggcaggga ccacaccatg gggcccagaa atcaaccagc
    10921 actttaccgg cacattctaa cacctcggcg ttaaaattag tgtcgtttac ttcaaaaata
    10981 gtgcccatag ttacctccac aaggaaatag taagatacta tcatagcata tgattttcaa
    11041 taattccaga agtttatttt tttgtttata caattatgct aaaaatacac gatgcagtca
    11101 ggaaatgtca atag
//
LOCUS       DAOOJH010000079        10772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5526_length_10772_cov_8.481291, whole genome shotgun sequence.
ACCESSION   DAOOJH010000079 DAOOJH010000000
VERSION     DAOOJH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10772
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5526_length_10772_cov_8.481291"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            569..913
                     /locus_tag="PLJ75_09660"
     CDS             569..913
                     /locus_tag="PLJ75_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05518.1"
                     /translation="MLKNNTIAAFLIIIIIIIIITTTILFPLFHHAAAIHVLNHENSV
                     EKNALKINCNTFHSNFTVIPRTTKINDVELSYEEIFSITKKLIAKNFLMDLTTFIVTI
                     AKYSFASKATFS"
     gene            1096..1224
                     /locus_tag="PLJ75_09665"
     CDS             1096..1224
                     /locus_tag="PLJ75_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HPD05519.1"
                     /translation="MGQDFDVEKLKGLSDDEVASRLSGEGYNELPQSKIRGSIYSA"
     gene            1422..2609
                     /locus_tag="PLJ75_09670"
     CDS             1422..2609
                     /locus_tag="PLJ75_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009303542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPD05520.1"
                     /translation="MELQGLTIGILKEIMENENRVAGIPETVKKMVDNGAKVLVESGA
                     GLGSFYTDADYKNAGAEIINDPESIYNSADVILKVKEPLMNEAKNKHEVDMMKSNQVL
                     ISFLHPSNPINHDMVKKLAQKGVIGFSLDCIPRISRAQSMDALTSMSTVAGYKAIISA
                     ACRLPKFIPMITTAVGMFRPAIVFVVGTGVAGLQSLATAKRLGAEIYAADVRPDAVEQ
                     SKSLGAKIIDTGVPPEIAIGEGGYARHLSDEWIHKERQSFYEIVKKADIVVLSALVPR
                     KIAPILVDETMVRSMKPGSVIVDIAIDQGGNCELTESGIICVKHNVTIDGTKNIPGSV
                     ALSASSMFAQNVLNFLSLIVKDGKININKSDEIIASALITENGKIVNPDVLEEIVSYK
                     EHR"
     gene            2609..2896
                     /locus_tag="PLJ75_09675"
     CDS             2609..2896
                     /locus_tag="PLJ75_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002670738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HPD05521.1"
                     /translation="MNNSIMILIFIISTVAGYKLIKDVPSLLHTPLMSGMNAFSGITV
                     IGAIVTTASAAALHSKILGAIAIILAMINVVGGFYITDRMLGKFKSGLKDK"
     gene            2901..4316
                     /locus_tag="PLJ75_09680"
     CDS             2901..4316
                     /locus_tag="PLJ75_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HPD05522.1"
                     /translation="MELNIVNIVSLVIILICAADIKLMSSPKTALLGNRIGALSMLGA
                     IVFVLIYNNIITISLLLVSLIIGAFIGVLFTYKTGGVKIPQLVALFNGFGGGASLLVT
                     FILLLEQGSYIDTFTRFTAFSGAIVGGLTFSGSLIAAGKLEGIINQKPVTIKAHSIIT
                     SLLLIILLVIMIFGTVIDNYIVTFASGATVLSLLFGVIFAIRIGGADMPITISLLNSC
                     SGLAASVCGFAVGDIILISLGAIVGASGLILTQIMCKAMNRSLWHILTGSTYRKVTDS
                     SMGTVVDLNSNDAEKEVHNYIEIVNKAKKIIIVPGYGMALSQAQEQVKLLFDYLITKG
                     KDVRFAIHPVAGRMPGHMNVLLAEVDIPYEYLYDIDTINPEFASTDVVIVVGACDVIN
                     PEAITAQGTPIYGMPILRVHEAKHVIVCNKDTKPGYSGVENSLYNKSNVTLLLGNAAD
                     TLAELISQLHNDLQSDTVLQN"
     gene            complement(4337..4915)
                     /locus_tag="PLJ75_09685"
     CDS             complement(4337..4915)
                     /locus_tag="PLJ75_09685"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPD05523.1"
                     /translation="MIARIDHVSIAVPDFEKAKIFFEALGAIPGAGAKDDATKFFWQI
                     FSLGDMSRIELITPTDKGSFLDGFIQSRGSGVHHITLQTYDIKTAIAKLEQLKIPYFG
                     YNEYPGGVWKEIFIHPKHAFGVLIQIAEFNADDWLSPEVKMPDGQFSITVNNGTVLLT
                     LPHPGGGTATINLTKEQAKKLAHNLLNSIDEK"
     gene            5133..5624
                     /locus_tag="PLJ75_09690"
     CDS             5133..5624
                     /locus_tag="PLJ75_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05524.1"
                     /translation="MDFETTTCISCNHLEILASYSEKMDVPLRTLIVYLIMYSAKHEK
                     NQVNAFKRIAYRQRNPDNPWKRLHLVMYYSEYEFFLDVKKLWKMSLAKIIEFCMENVL
                     DEFIQYFSKLIKEINTDNYPDNFPSYYENRSYTFDFHREKGIHCLKFYWGPPPEILGK
                     QNI"
     gene            5755..7317
                     /locus_tag="PLJ75_09695"
     CDS             5755..7317
                     /locus_tag="PLJ75_09695"
                     /inference="COORDINATES: protein motif:HMM:NF020022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD05525.1"
                     /translation="MQQIVSFLSKPSSYNHAVDTVHVIQTHISYVFIAKPFVYKVKKP
                     VDFGFLNFVSFEDRKFYTFEEYRLNARISPEIYIGVVVVIKQGASYTLIEDSNYNESD
                     VVAYCVKMRYIDDAYSLKNLLQHGLDDSLLSLIAKKIYTFHSIADNTEGLQGFGGIAE
                     ISKNSLENFDQIEKYIDVIIPRTEYVFMRQWTEHYIEQNKNLFNERNLKGFVRDCHGD
                     LHCEHVYSINGEIVILDCIEFNKRFRYIDTISDIAFLLMDLDVNNKTTESNKLCAQYV
                     QLSGDYDGSVLLPFYKAYRAMVRAKINSFQSDNEGITARERDACVQKAMQYFHLAKSY
                     MQHHIKPVVYIVFGNIGSGKSTIAMELASLTGGIVINSDAVRKKMAGLDVYDSARAKA
                     GEGIYNPDMTTKVYETMNEKALAIVAGGITAILDATFYSRQLRQQLHRYFADHNIATQ
                     FVYVDCSHDELIKRIKTRQQDKSISDATVDIFNQLKDRFEKPDNEIPVTIINSEKDMD
                     EIINQLKNIIVK"
     gene            7331..8248
                     /locus_tag="PLJ75_09700"
     CDS             7331..8248
                     /locus_tag="PLJ75_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05526.1"
                     /translation="MNHVIDIVLCLLLVQTNATNFQKFNECITNGKFEEAKIVLDSWG
                     ENKKSDPQYYICNFNYYINKSIINGTVVQVNPPDDKTSIMMVLKDPKTGNIAGYMIYK
                     KMYNNEVASEGIKYIKEGIKKFPDHYEMRFGLLWALKEMRQLDEYLIELEDALKYVTN
                     NNLQKVYWNNNESISNPHEFVIEKVQGSLNDIIDSNEIFSNQQFLHKYVDLMIQYFPK
                     HKYGYADKGYIYLVNNDYGHALQYYLKANKIDESDVLILCNIGIVYKMLQDYDKAMIY
                     FEKVIQIGNDTYFVNEAHKELDAMKKAGN"
     gene            complement(8308..9468)
                     /locus_tag="PLJ75_09705"
     CDS             complement(8308..9468)
                     /locus_tag="PLJ75_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPD05527.1"
                     /translation="MKQLYGALPIIVILLSLSFVDTKLFCKNLLELYSNPVYTEEGLQ
                     NAINFDPDSDSIFLPKINNKDLFTAIDDLSICRIPDVRRFLYIYLTKGREYTINAIKR
                     STLYISIIHEQMKEYPDMPEDIALLPLLESAFNPYAVSKSGAAGIWQFIPGTARVLGL
                     QINNFVDERRDIVKSTDAALRHLKHLHNSLGSWELALLAYNGGSGYLTRTMKSNGYHH
                     FYDLIEAGVLHKETQEYVYRYAALALIYKYPELFSIEDELQKPEIEYNVEQVTLIYPV
                     KIDVLIEKCNIPREVITLYNPQLKHSITPPKERNYILLLPKGSKEKIEEYKEEIYTLK
                     FTKINTHRVKSGETLLQIAKRYRASPKMIIFINNLRNPDKLYPGDILYIPVL"
     gene            complement(9804..10175)
                     /locus_tag="PLJ75_09710"
     CDS             complement(9804..10175)
                     /locus_tag="PLJ75_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05528.1"
                     /translation="MQTIQIYLLNENEETIENLIAINQQIIEEISLSNYSISNIVKII
                     TLKIGVEYNEFEKFLLNLKHPIIDSIFNSMDTIDIIAKRLSNLNETITILLKKHQDLI
                     LKNIHEINKIIELQYTISYLS"
     gene            complement(10252..10686)
                     /gene="fliS"
                     /locus_tag="PLJ75_09715"
     CDS             complement(10252..10686)
                     /gene="fliS"
                     /locus_tag="PLJ75_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HPD05529.1"
                     /translation="MALPARNPYDQYKHTEINTANQGKLIVMLYDGAIKFCNIAIENM
                     QPKTYDIANINIIKAQDIINELILALNMNDGGDIARNLFNLYIYFKKQLLEANIQKNS
                     DILKNVVKLLKELRDAWDKISAQETPSDRVATASKGSFSIEG"
BASE COUNT         3546 a         1602 c         1961 g         3663 t
ORIGIN      
        1 ccactccaag gaaataaaaa tattttgaat ctaaaccagc ttaacaatta ttgtacttgt
       61 caatcatggt cgcttctctt tgctcatttt acctaatagt gccctcaaat cccattaaaa
      121 aatgagttga gaaaataagc attctacatc actttattac ataataacat ggcaacatcc
      181 agctatgccc tgataataag taaaatatca ctttgcttta ctttgtatgt ataaatggaa
      241 ggggaccgct ttgtgatatg tgatatgctt gtagcaaaac gccactttaa ttattgaacg
      301 ggtgatcata acgctattta atgtgtaagc ttgggttaag cactgcaaag ttgaactagc
      361 gaagtgacaa aaaatttgtt gacaattttg taatattttt tctttattct atagttaagg
      421 caaaaagtag aatattgttt taagaacagt cgatttcggt atagccgaat gcagacgggc
      481 ctgaaaaggc agcagctaaa ccctgcggga cttgatccac ggaggatttt ttagtctttg
      541 gatgtaccca taaactggtt gattggatat gctaaagaat aatacaatag cggcttttct
      601 tattattatt attattatta ttattattac tactactata ctgtttcctc tattccatca
      661 tgctgcagca attcatgtat taaaccatga aaattctgtt gaaaaaaatg ctcttaaaat
      721 taattgcaac actttccatt caaatttcac agtaatacca cgaactacaa aaattaatga
      781 tgtagaatta tcttatgaag aaatattttc aataacaaag aaactcattg ctaaaaactt
      841 tttaatggat ttgactacat ttatagtaac tatcgccaaa tattcatttg cctcaaaggc
      901 tactttctct taaattacat ctcacattta taactgttac tttaagatga ccgggtacat
      961 cccttatgca tgtaataaaa tctgtcagag taatggtatg gttttatatg aatacactac
     1021 aaggggcgat atagtttttt tgtgtcttta aaaaattgat gaacaaagaa attgcaatga
     1081 tactacgagg gtgctatggg acaagatttt gatgttgaaa agcttaaagg tttgagtgat
     1141 gatgaggttg cttcacgttt gtcaggtgaa ggatataatg agcttcccca gtcaaaaata
     1201 cgtggtagta tttattctgc ttgataacag ctgtgcttat agtagtaatc aatgagattg
     1261 cgaaattacc attatttatg cagttgtttc ataaaaataa cgattaaagt cggtgaaact
     1321 gtaacaataa attgtttttt tcttgacaaa aattaaattt ttattattat gtatatagtg
     1381 agtgagcgct tactcactat tattgtaaat aggagaacgc catggaatta caaggtttaa
     1441 ccattggtat tcttaaagaa ataatggaaa acgaaaatcg tgttgctggt atccctgaaa
     1501 cggttaaaaa aatggtggat aatggtgcta aagttttagt tgagtcaggg gcagggttgg
     1561 gttcttttta tacagatgca gattataaaa atgctggtgc agaaattatt aatgatcctg
     1621 aaagtattta caattcagcc gatgttattc taaaagtaaa agaaccgctg atgaatgaag
     1681 ctaaaaataa acacgaagta gatatgatga aatccaatca ggtgctgata tcatttttac
     1741 atccatcaaa tcccataaat catgatatgg tgaagaaact tgcccaaaaa ggtgttattg
     1801 gattttcact ggattgtata ccaagaatat cgcgagcgca atcaatggat gcattaacat
     1861 caatgagcac tgtagcagga tataaggcaa taatttccgc agcctgtcgc ctgcctaaat
     1921 ttattcccat gattactact gctgtaggaa tgtttcgccc tgctattgtt tttgtagttg
     1981 gaaccggtgt cgcagggcta caatcgttag caacagcaaa aaggttaggg gctgaaattt
     2041 atgctgcgga cgtcagacct gatgctgttg aacagtcaaa gagcctgggt gcaaaaataa
     2101 ttgatactgg tgttccacca gagatagcaa ttggtgaagg tggatatgcc aggcatctat
     2161 ccgatgaatg gatacataaa gaaagacagt ctttctatga aatagtaaag aaagctgata
     2221 tagtggtact atcggcactt gttcccagga aaatagcccc catccttgtt gatgaaacga
     2281 tggtacgctc aatgaagcca gggtcagtta ttgttgatat agctattgat cagggtggta
     2341 attgtgaact tacagaatcg ggtatcatat gtgtcaaaca taatgttacg attgatggta
     2401 caaaaaatat tcccggatcg gtagccttga gtgcaagttc aatgtttgca caaaacgtat
     2461 taaacttttt atcactcata gtaaaagacg gtaagattaa tataaataaa tcggatgaga
     2521 ttattgcgtc agcattaata actgaaaatg gtaaaatagt aaatcctgat gttttagaag
     2581 aaatagtgag ctataaggag cataggtaat gaataatagt attatgatac ttatttttat
     2641 tatatcaacg gtagcaggat ataaactaat taaagatgtt ccttctttac tgcatacacc
     2701 gcttatgtca ggcatgaatg cgttttccgg tattactgtt attggtgcta ttgtaacaac
     2761 agccagcgca gcagctttac atagtaaaat acttggcgct attgcaatta tattggcaat
     2821 gataaatgtt gtaggtggat tttatattac cgacagaatg cttggaaaat ttaaatcagg
     2881 attaaaagat aagtaatgat atggaattga atattgtaaa tattgtttca ctggtaatta
     2941 ttttgatatg cgctgctgat atcaaattga tgagttcgcc aaaaacagcg ttgttaggga
     3001 atcgtattgg tgctctttca atgcttggtg caatagtgtt tgtgcttatc tataacaata
     3061 ttatcaccat ttctttgcta ttggtttcat taatcattgg cgcatttatt ggagttcttt
     3121 ttacctataa aacgggtggc gtaaaaatac cacagctcgt tgctttattt aatggatttg
     3181 gtggaggggc gtcgttactg gttactttta ttcttttact ggagcaaggt agttatattg
     3241 acacatttac acgttttaca gctttttcag gtgctattgt tggcgggctt actttttcgg
     3301 gtagcctgat agctgctggt aaattagagg gtataattaa tcaaaaacct gttacaataa
     3361 aagctcactc aattatcact tcattactgc ttattatttt actggtaata atgatttttg
     3421 gtacagttat tgataattat attgttactt ttgcatcggg agctactgtg ttaagtttat
     3481 tatttggagt aatatttgca atccgaattg gcggagcaga tatgcccatc actatctcgt
     3541 tattaaattc atgttcaggt ctggctgctt cggtttgcgg atttgctgtt ggggatatca
     3601 tcttaatttc gttaggtgca attgttggag catcagggct tattcttacg caaattatgt
     3661 gtaaggctat gaatcgatcg ttgtggcata ttctgaccgg ttccacatat cgtaaagtga
     3721 ctgattcatc aatgggaact gtagttgatt taaattcaaa tgatgcagaa aaggaagtac
     3781 ataattatat tgagatagtt aataaagcaa aaaaaattat catagtacct ggctatggca
     3841 tggcattgtc acaagctcag gaacaggtaa aattactttt tgattattta ataacaaaag
     3901 gtaaggatgt gcgttttgct attcatcctg ttgcaggcag gatgccaggt catatgaatg
     3961 tccttttagc cgaggttgat atcccgtatg aatacctcta tgatattgat accatcaatc
     4021 ctgaatttgc atcaactgat gtcgttatag tagttggtgc atgtgatgta ataaatccgg
     4081 aagccattac tgcccaggga actcccattt atggtatgcc tatactacgt gttcatgaag
     4141 caaaacatgt gattgtttgc aataaagaca ctaaacccgg gtattcaggt gtagaaaatt
     4201 cattatacaa taaaagtaat gttactcttt tgttgggtaa tgctgccgat acgctggctg
     4261 aattaataag ccagcttcac aatgatttac aatcagatac tgttttgcaa aattaaggta
     4321 tctgaattgt agtttattat ttttcgtcaa tactgttgag aagattatgt gctaattttt
     4381 ttgcctgttc ttttgtcagg tttattgttg ctgtacctcc acccggatgt ggcagtgtta
     4441 gtaaaaccgt accattgttt accgtgattg aaaactgccc atccggcatt tttacttcag
     4501 gtgacaacca atcgtcagca ttaaattctg caatttgaat aagtacacca aaagcatgtt
     4561 ttggatgaat aaaaatttct ttccatactc cacccgggta ttcgttatat ccaaaataag
     4621 gtatttttaa ttgctccaat tttgctatag cagtttttat atcatacgtt tgcaatgtta
     4681 tatggtgcac accgcttcct cgtgattgta tgaatccatc taaaaaactt cccttatcgg
     4741 ttggtgtgat aagttcaatg cgactcatgt cacccagaga aaatatctgc cagaaaaatt
     4801 ttgtagcatc atcttttgct ccagcaccgg gtattgcacc taatgcttca aaaaatattt
     4861 ttgctttttc aaaatctggt acagcaatcg atacatggtc tatgcgggct atcataaaag
     4921 caacctcgtt attgttttgt taaaaggtaa tactaatggt attgataaaa atttcaaatc
     4981 atttttaagc gatagttatt tataaattaa ctatcgacaa aaaaataatt gcattgttat
     5041 agggtaacgt attaagctca gtataaagag atgctatagt ccagacgatg gcgatatatt
     5101 tttttaaaat taaaaacatt tgggaggaaa taatggattt tgaaactact acatgtattt
     5161 catgcaatca tttagaaata ctggcaagct attcagaaaa gatggatgtg ccactgcgga
     5221 cattgatagt ttatcttatc atgtattctg caaaacatga aaaaaatcaa gttaatgcat
     5281 tcaaacgtat agcctaccga caaagaaatc ctgataatcc atggaaaaga ttgcatcttg
     5341 ttatgtatta ttcagaatat gaatttttct tagatgtaaa gaagctgtgg aagatgtcat
     5401 tggctaagat tattgaattt tgtatggaaa acgtactgga tgaatttatt caatatttca
     5461 gcaagcttat taaagaaatt aataccgata actatcctga taattttccc agttattacg
     5521 aaaataggtc atatacattt gattttcacc gggaaaaagg catccattgt ctcaaattct
     5581 actggggacc accgcccgaa atactgggaa agcaaaatat atgaacgggt aaaatttttt
     5641 gtaaaaatgt tttaaaaaat ttcttgccaa tttactggat tgtactcttt attttgttgc
     5701 taccatacat gcatctattg tgctatatat caatgctata agtacgaggt taatatgcaa
     5761 cagattgtat catttctttc aaagccatcg tcgtataatc atgccgttga tactgtacat
     5821 gttattcaaa cacacatatc gtatgtattt attgcaaagc catttgtgta taaggtaaag
     5881 aaacctgttg attttggttt tttaaatttt gtatcttttg aagatagaaa attttataca
     5941 tttgaagaat accgtttaaa tgcacgaatc agccctgaaa tatatatagg cgtagttgtt
     6001 gttataaagc agggagcatc gtatacatta attgaagata gtaattataa tgaatcggat
     6061 gttgttgcct attgtgtaaa aatgcgatac atcgatgatg catattcact gaagaattta
     6121 ttgcagcatg gattggatga cagcttacta tcgctcatag caaaaaaaat ttacacattt
     6181 cacagtatag cggacaacac cgaaggctta cagggatttg gcggcattgc tgaaattagc
     6241 aaaaactcac ttgaaaattt tgatcagatt gaaaagtata tagacgttat aataccgcgc
     6301 accgaatatg tatttatgcg tcaatggacg gaacattata ttgagcaaaa taaaaattta
     6361 ttcaatgagc gtaacctcaa ggggtttgta cgcgattgcc atggcgatct gcactgtgag
     6421 catgtctact ctatcaatgg ggagatagtt attcttgatt gcattgaatt taataaacga
     6481 tttaggtata ttgatacaat ctctgatatt gcttttttac ttatggatct tgatgtcaat
     6541 aataaaacaa ctgagtctaa caaattatgt gcacagtatg tacagctatc gggtgattac
     6601 gatggcagtg tgctgttgcc attttataag gcatataggg caatggtgcg agctaagata
     6661 aatagcttcc aatcagataa cgaaggaata actgcacggg aacgcgatgc atgtgtacaa
     6721 aaggccatgc aatattttca tttagcaaaa agctatatgc agcatcatat aaagcctgta
     6781 gtatatatag tctttggcaa cattggaagc ggcaaaagta ccattgcaat ggaattggca
     6841 tcgcttaccg gtggcattgt tattaattct gatgctgtcc gtaaaaagat ggcagggctt
     6901 gatgtttatg acagtgcaag ggcaaaagca ggtgagggta tctataatcc tgatatgacg
     6961 acaaaagttt acgaaaccat gaacgaaaaa gcgttagcga tagttgctgg tggaattacg
     7021 gcaattctgg atgctacttt ttatagccgg cagttgcgac aacagcttca ccgctatttt
     7081 gctgatcata atatagctac acaatttgtc tatgttgatt gtagccatga tgagctaata
     7141 aaaaggatta agacacggca acaggataaa tctatttcgg atgcaacagt tgatattttt
     7201 aaccagctta aagacagatt tgaaaaacca gacaatgaga taccggttac tataattaac
     7261 tctgaaaaag atatggatga aattataaat caattaaaaa atataattgt aaagtaagga
     7321 gcatgctgct atgaaccatg ttattgatat agtgttatgt ttattacttg tgcagacaaa
     7381 tgcaacaaat tttcaaaagt ttaatgagtg tattacaaat ggcaaatttg aagaggcaaa
     7441 gattgtttta gatagctggg gcgaaaataa aaaatccgat cctcaatatt atatatgtaa
     7501 ttttaactat tatattaata aaagtataat caatggaact gtagtacagg taaatcctcc
     7561 tgatgataaa acatctataa tgatggtatt gaaagatcct aaaacaggta atattgctgg
     7621 ctatatgatt tataaaaaaa tgtataacaa tgaggttgcc agtgaaggga ttaagtatat
     7681 taaagaaggt ataaaaaaat ttcccgatca ttatgaaatg cgatttggat tattatgggc
     7741 attaaaagag atgcgtcaac ttgatgaata tctaatagag cttgaagatg ctttgaaata
     7801 tgttaccaat aataatctgc aaaaggttta ttggaataat aatgaatcca tatccaatcc
     7861 acatgaattt gtgattgaaa aagtccaggg tagtttaaat gatattattg actcaaatga
     7921 aattttttca aatcaacaat ttttgcataa atatgttgat ttaatgatac aatatttccc
     7981 aaaacataaa tatggctatg cagataaagg ttacatctat ctggtaaaca acgattatgg
     8041 gcatgcgttg caatattatt taaaagccaa taaaattgat gaatctgatg tattgatatt
     8101 gtgcaacatt ggtattgtgt ataaaatgtt acaagattat gataaagcca tgatatattt
     8161 tgaaaaggtt atacaaattg gcaacgacac atattttgtc aatgaagcac ataaagaatt
     8221 ggatgcaatg aaaaaggctg gcaattgatg caacgcttta ttgaataaag cataacgcga
     8281 gattgtttgc aaacataaat tcaattatta taaaactggt atatataaaa tatcccccgg
     8341 gtataattta tcaggattgc gaaggttgtt tataaagata atcatttttg ggcttgccct
     8401 atagcgttta gcaatctgta ataatgtctc ccctgatttt actctatgag tatttatttt
     8461 tgtaaattta agtgtgtata tttcttcttt atattcttca attttctctt tgcttccttt
     8521 tggtaatagc aaaatatagt tcctttcttt tggaggggtt atactatgtt ttagctgggg
     8581 attatatagt gttatcacct cacggggtat attgcatttt tctatcaaaa catctatttt
     8641 tacagggtag attaatgtaa cctgttctac attgtactct atttcaggtt tctgtaattc
     8701 atcttcaatg gaaaacaatt caggatattt ataaataagt gcaagggcag catatctgta
     8761 aacatattcc tgtgtttctt tatgtaatac accagcttct ataagatcgt aaaaatggtg
     8821 gtacccattg gatttcatag tacgtgttaa ataaccagat cctccattat acgccagtag
     8881 tgctaattcc catgaaccta atgaattatg taaatgttta agatgacgca gggctgcatc
     8941 ggtggatttc acaatatcgc gacgttcatc aacaaaatta ttaatctgta atcccagtac
     9001 tctggcagta ccgggaataa actgccatat tccagcagct ccactttttg agacagcata
     9061 ggggttaaat gcactttcaa gcagggggag cagggcgata tcttcgggca tgtcaggata
     9121 ttctttcatc tgctcatgta tgattgatat atagagcgtg gaacgcttta tagcattgat
     9181 ggtatattct cttccctttg tgagataaat atataaaaat cttctcacat ctggaattct
     9241 gcagatggat aaatcatcaa ttgcagtaaa taaatcctta ttgttaattt ttggtaaaaa
     9301 tatactatcg ctatccggat caaaattaat agcgttttgt aatccttctt ctgtataaac
     9361 aggattagaa tataattcca gtaaattttt acaaaaaagc tttgtatcaa caaatgataa
     9421 tgacaatagt ataactatta taggcaatgc accatacaac tgtttcattg atcagacctt
     9481 tctatagtgt tattatacat tactaaacaa atgtaatatt tgctatatta aaattagtat
     9541 tagttttata tagcagataa tggttgtaat ttcaaaaata atgtatagta aaaatagaca
     9601 ccagagattg attgctgata ttttgctccc ataaaggacc tacacattca gtttctacgt
     9661 gcagacctat tattgtaaag tgattaatct gaataatcac atatgatttt atttaataca
     9721 atttttatat tgttgatgaa atacatgagg aaaggggatt gcattatgaa tactattttt
     9781 ttaagtttaa ttattacaaa caattatgat aaatatgaaa tagtatattg taattctata
     9841 attttgttta tctcgtggat gtttttcaaa attaaatctt gatgtttctt taatagtatt
     9901 gtaattgttt catttaggtt tgataatctc tttgcaataa tatctatggt atccatacta
     9961 ttaaaaatac tatcaataat gggatgtttc aaatttaaaa ggaatttttc aaattcatta
    10021 tattctacgc caattttcaa tgtaatgatt tttacaatat ttgaaattga ataattggat
    10081 aaagatatct cttcaattat ttgttgattt attgcaatta aattttcaat agtttcttca
    10141 ttttcgttta ataaataaat ctggattgtt tgcatattta tttctatctt ttttaaaaga
    10201 ttttgttttg tatttaacaa atcaagtaag gaatagagaa gtatttgtat gtcatccctc
    10261 aattgagaat gaacctttgg atgctgttgc aactctatcc gaaggagttt cctgagcaga
    10321 tattttatcc catgcatctc gtagctcttt taatagttta acaacatttt ttaatatatc
    10381 agagtttttc tgtatatttg cttctagtag ttgctttttg aaatatatgt aaaggttgaa
    10441 aagatttcgg gcaatatcgc caccatcatt catattcaat gctaatatca gttcattgat
    10501 gatatcctga gctttaataa tattaatatt ggcaatatca tacgttttag gctgcatatt
    10561 ttcaatggca atgttgcaaa acttaatagc accgtcatag agcattacta ttaattttcc
    10621 ctgattggca gtattgattt cggtgtgctt gtactggtcg tatggatttc tagcaggtaa
    10681 agccatttat cccccctcga tcgattttca acacattggc attactagta tcggataaaa
    10741 atatataaaa ctttattgaa taaagcaata gc
//
LOCUS       DAOOJH010000080        10706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5562_length_10706_cov_9.644822, whole genome shotgun sequence.
ACCESSION   DAOOJH010000080 DAOOJH010000000
VERSION     DAOOJH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5562_length_10706_cov_9.644822"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLJ75_09720"
     CDS             complement(<1..431)
                     /locus_tag="PLJ75_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05530.1"
                     /translation="MKKIMFIIIFLYSASCISYAQEKRTMQQEADDKKFQQGLFFLSI
                     NEKQKALNEFYEYLDIYVDGLYRAKAYRFIAAIYFDNANFTKAIAIYKRLFEEFPLDD
                     DGMYGLLQIGICYSKMGFDEEASKTFNQLMKEFPDSSYAKDA"
     gene            complement(507..1037)
                     /locus_tag="PLJ75_09725"
     CDS             complement(507..1037)
                     /locus_tag="PLJ75_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD05531.1"
                     /translation="MKSYKTYIDETILIKLQDLSLFKGLSQNVIRNIALVCTVKKIKR
                     GKCIIKEGDMGDELYIIVNGEIEIQKRTLQNEPYTVVTMNANETPLYVGELAMIDNDK
                     RSATVIAKQDCDCLVLKRKDFIKFGDRNPKEGLILTRAIALKLAQSLRRTNDDVITLF
                     SALVDEISGETEPGKS"
     gene            complement(1047..2252)
                     /locus_tag="PLJ75_09730"
     CDS             complement(1047..2252)
                     /locus_tag="PLJ75_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPD05532.1"
                     /translation="MKIYTEIKIILYNLFHSMVLKIFGVCFTLLFIFSTTIYYVERGY
                     VRTIKSHGQQETVQSNINSIEDSVWWAIVTSATVGYGDKYPISRLGRFIGILTIFFGM
                     ALVGLITGNIASTLVERQLKEDRGLKQVKIKNHYVICGWKKDMAMVLSNIAMRQGDFT
                     GYDIVLVCMIDPENIENLKSNILFSNIHYVYGDHIDEHVLNRANIQYAKKVLVLADEL
                     GKSGQDADSRTVMSVMSIKAISRSVYVCAEIIDQKYERYLRLSGCDEVILSNNYNKNI
                     IADAFAKSGLAHVIGELVDVSTPVNLTTEDISQHYIHKTYGELFDHYKTKNKILIGLL
                     ENTGNFYDRKRLALQEAQKTPDISRLVDNLRMVKSMMSNYPVLNPKKSYIIKNFSKAV
                     LIEGSTENG"
     gene            complement(2431..4371)
                     /gene="cfpA"
                     /locus_tag="PLJ75_09735"
     CDS             complement(2431..4371)
                     /gene="cfpA"
                     /locus_tag="PLJ75_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic filament protein CfpA"
                     /protein_id="HPD05533.1"
                     /translation="MSITQFPKSPNKVHPTEPSAVGSRNSLAQEGRDKVAEARLMVDE
                     AVDKLVNHVQTKLPEEVLKKLDVMGGLKEKLYNYVNQSYVNMFNRYTVTMEDEFIKKV
                     RDFVDREEAKGLARYTPREIMELLDKIGGVDKFNTGEIEKSIVNMYGHLQGHIQRGLN
                     DLENETNAILRQKTDVGAFIRGENAYAIIKCVFKDNELRPKYVNDVKLSVNILDSELI
                     SQIYHYQVTVESLLKDAIQKHIHELIDKQIQILKEELIDQGKSELTGTEVMFEKIKRI
                     ENFTDDEKEDEKSKRYTILAKKFLDKIEGLRAEIDVEEYDPLNIRENIKYIIDEENIR
                     NRGYNTAVNAITSILDTSKLGYQVCDNLKNARVCQIREYEELDKSVLPDERYAIRLAY
                     YDQNQLREAKKEFDRQMDSFTREILRVWDVAHAHYESKKRFRSLRDFDDLSNRLMSRD
                     WRRNKHDEDADPNSVLWNEIGEMYNENSFVEKNNRTYEDRIHNLKNKLKYLREMLHKM
                     HGFQNPIERVILDERINFLERRFNEFTYKINPHHIQPGLVLDVDVTTIKRKQYMLKGM
                     ANVLNEFLYSVSRGFQDAAFATFKRRRSTVREDIDQSFSQTEMKEDIFEAAYKAQSGS
                     EIKGSVDLSSSKSKPKRGLKEL"
     gene            complement(4553..5659)
                     /locus_tag="PLJ75_09740"
     CDS             complement(4553..5659)
                     /locus_tag="PLJ75_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD05534.1"
                     /translation="MKVTVQYFRLIIAIIIFAGLCYADGYAKSRDLILVIDTSLSMAG
                     YGGKNIMPLVKQSLPKFIEQLDENDSFTLITFSTDVKLYPTVYIKHKSNKDILNKYIS
                     MVEASGKWTYTMAMITAAFDKAKQLQMEDPDREQVIIIITDAIDDPPPDARKYHYDIK
                     NIARQYSGNEWFVFFVNLGQIQKDSRLEKIQKEVKESISPYTKVIDAQESVDKIIVKD
                     LKTNIDTMAKIKEIHSRPLYMHPLFIIIVAIVLLIILFMVLRRAAQLKVKGALEYWNN
                     ELLEPSIEKANLTAKNSRTITIGSDTHNMIRIHDLAIREPIMLKAVRKDKVVKCQLLA
                     PENVVQFVNRDAGNILNDGDIFKINNYTFKYYEG"
     gene            complement(5807..6514)
                     /locus_tag="PLJ75_09745"
     CDS             complement(5807..6514)
                     /locus_tag="PLJ75_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05535.1"
                     /translation="MLKEYFASIQNTISGLQSSKDGKKASKKNQVREEFDGKKIMKLL
                     QDVVPNEFIVSDKKPVDASGYVPKTVDYIAYYKKWAIVEEMMNGHIPSELVYATFHIT
                     KHIDKQGLSEVLSNVILTKKLNKYAELDQDIIIPAFVIVQASDIDVPQLKNDVIDYYV
                     DHSVDSMFEFDIMMIIGKGIIIKDWREKRKFVALETEEDTLLWFFILINEYLDVDVKR
                     SLDLRHYVKETRRYREY"
     gene            complement(6721..>7485)
                     /locus_tag="PLJ75_09750"
     CDS             complement(6721..>7485)
                     /locus_tag="PLJ75_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05536.1"
                     /translation="HLYLKNGEIQKAKRMFDIAINEDSTNEEAILGRARSLKHLGKDA
                     EAYNHYDYFLRYYGAVSQYANDVKNAYKVQAYKSGLNAFRSGRYNDAIGFFNRVLIHF
                     GGDKLSENALYWIGESYFAMRQFDRAIAYFNKVLTNDFYHKDQDAQIKKGYAYFMQKK
                     FDLAAREFQRYIREFPNGKYVAVAKEWKNMSTAELQNIIESKKAESEGYIEEELKQKP
                     QPQKNNTNENDLGLEEDEEVVGSVSQDFNDVNVAEF"
     assembly_gap    7486..7585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7586..7821)
                     /locus_tag="PLJ75_09755"
     CDS             complement(<7586..7821)
                     /locus_tag="PLJ75_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05537.1"
                     /translation="MKTKTLAGALLIVIGILLIIYYVLVIQPQSEAKKLLTEATLIYE
                     RNDKESINQAVTILTKIIARYPDTKTAIEAYYYIG"
     gene            complement(7850..>9412)
                     /locus_tag="PLJ75_09760"
     CDS             complement(7850..>9412)
                     /locus_tag="PLJ75_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05538.1"
                     /translation="RTTRVPSTPEVALQATTVKVVLAGAEPVAIDSNLPPGQYYYVVL
                     ARDKIVEKDIELYPDVNYTSAPIVIKKKIPELPAKRLYDHVTLLYARIVNKTQVLLTW
                     KGIQQPNIVYNVYRATEPLNSPGKIKNARLVGEIQSDVESYIDRTIAQTGKYYYAVTV
                     KDMEGNEDIQLIPDQSYTSAPVSVILRRDVFVTGIKATLDNDYLKVSWNKPDIDVKNF
                     VIYRHTEPITDAQDLALATKVATVDAKRSHYIDTHPIPGTYYFAVLAILEDSSIDTTL
                     KPDMNYTTVPITVGHPISLKLLEAIEQNGLVKISWAYTGSSGNRYFKLFRLRNAPFSV
                     QDLNNAYLVDVINITNNEYIDSNIPAGSYYYALVPETYDNELKIIKGVNCTAESVKVT
                     KMAKAQLDLQDPEKEKTGKSELPESQKEEAKPYQLEKQKLSATADINKIIAITFYKGK
                     YETCIDTLSKYSEQSKNSIEIAKANLYIGRSYIELKQYKKALHYLMQKDVATYFPEDA
                     KFWQDFAIARSQ"
     assembly_gap    9413..9512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9811..10401
                     /locus_tag="PLJ75_09765"
     CDS             9811..10401
                     /locus_tag="PLJ75_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD05539.1"
                     /translation="MDKQEKIHKKKIIILEALKRLLQNDVYSRITVQAVADEAGFSKG
                     GLLHYFPTKEEMYIEFMEYLFNEINNDHKNVLKGNLHSKAKASISAMYGVERFFLDPA
                     TAKILINLMLYAYEDEKIMVRLKEFIRRHLELYKTIINEAREDQPQRRKTDFDAEFHA
                     RIAQTIVMSAGLFESIDPLPIDNLNFMRYIISLFRG"
BASE COUNT         3232 a         2012 c         1445 g         3817 t
ORIGIN      
        1 gcatcttttg catagcttga atcgggaaat tctttcataa gctgattaaa tgttttagat
       61 gcttcttcat caaatcccat tttgctgtaa catattccaa tctgtaacaa accatacatc
      121 ccatcatcat ccagagggaa ttcctcaaaa aggcgtttat aaatggcgat agccttcgta
      181 aaattggcat tatcaaagta tatggctgca ataaagcgat aagcttttgc tctatatagt
      241 ccatcaacat aaatatcaag atattcataa aactcattta atgctttttg tttttcatta
      301 atcgatagga aaaacaatcc ctgttgaaac tttttatcat cggcttcctg ttgcatggta
      361 cgtttttcct gtgcataaga gatgcaactt gcactatata aaaatattat tataaacatt
      421 atctttttca ttgtttcttc tccaaaaatg aattactccc aatcttcaaa aacaaactca
      481 gattcaaaaa aaatatcgtt tccattttat gatttaccag gctcagtctc tcctgaaatc
      541 tcatcaacta aagcactaaa aagtgtaatt acatcatcat ttgttcgtct aagactttga
      601 gctaatttca gggcgatagc tcttgttagt atcaagcctt ctttaggatt tctatcgcca
      661 aatttaataa aatcctttcg tttcaaaacc agacaatcac aatcctgttt tgcaataact
      721 gtagctgaac gtttatcgtt gtctatcatt gcgagctcac ctacataaag aggtgtttca
      781 ttagcattca ttgttactac agtatatggc tcattctgca atgttctctt ctgtatttct
      841 atttcaccat tcacaataat atatagctca tcacccatat caccttcttt aattatacat
      901 ttgccacgtt taattttttt tacagtacat accaatgcta tattgcgtat tacattttga
      961 cttaaccctt taaagagcga aagatcctgt aattttatta aaattgtttc atcaatatat
     1021 gtcttatatg acttcatcac attacctcaa ccattttcag ttgaaccttc aataagcact
     1081 gcttttgaaa aattcttgat aatatacgat tttttagggt ttagtacagg ataatttgac
     1141 atcatgcttt tgaccattcg caaattatcc accaaccttg aaatatcagg tgtcttttgc
     1201 gcttcctgca atgcaagacg tttacgatca taaaaattcc cggtattctc aagaagtcca
     1261 attaatattt tgttttttgt tttataatga tcaaaaagct ctccatatgt tttatgtatg
     1321 taatgttgag aaatgtcttc tgttgtcaaa tttaccggag tgctgacatc aaccaattca
     1381 ccaattacat gagctaaccc actttttgca aaagcatctg caataatatt tttattgtag
     1441 ttgtttgata gtattacctc atcacatcct gatagcctta aataccgttc atatttttga
     1501 tcaattatct cggcacatac ataaacactt cttgaaattg ctttaattga catcaccgac
     1561 ataactgttc tggaatctgc atcctgccct gatttaccca actcatcagc aagcaccaat
     1621 accttctttg catactgtat attggcacga tttagcacat gttcatcaat atgatcccca
     1681 tatacataat gaatatttga aaataatata ttacttttta aattttcaat attttcagga
     1741 tcaatcatgc aaaccaacac aatatcatag ccagtaaaat ctccctgtcg catagcaatg
     1801 tttgataata ccattgccat atcttttttc caaccacaga taacataatg atttttaatc
     1861 ttcacctgct ttaatcctct gtcttctttt aattgtcttt ctactaaggt tgatgcgata
     1921 ttaccagtaa tcaaacctac cagagccatt ccaaaaaaaa tagtcagtat gcctataaat
     1981 ctgcccaatc gtgaaatggg gtatttatct ccatagccaa ctgttgctga tgttacaatt
     2041 gcccaccaca ctgaatcttc aatagaatta atattgcttt gaactgtttc ttgctgaccg
     2101 tgacttttta ttgttctaac ataaccacgt tctacataat aaattgttgt agaaaaaata
     2161 aacagcaatg taaaacatac accaaaaatt tttaatacca tgctatgaaa tagattatac
     2221 agtattatct ttatctcagt gtatattttc atgtttttta aatttgcttc catactatct
     2281 ttaacctgca tattacagta taattcatat caattatcga tagttattat aaaaaatatt
     2341 acaaaaatat tatgtcgctt agtattatag tgaaaaaaaa actgctcctt tacagagcag
     2401 tttttttgaa tatgtatata attttacttt ttacagttct ttcaaacctc ttttgggctt
     2461 gctctttgac gatgatagat caaccgaacc cttgatctcg cttccgctct gagccttata
     2521 tgcagcttca aaaatatctt ctttcatttc tgtctgagaa aatgactggt caatatcctc
     2581 gcgcaccgtt gatcttctgc gcttaaatgt tgcaaatgca gcatcctgga atcctcgtga
     2641 tacactatag aggaattcat ttaaaacgtt tgccatccct ttgagcatat actgtttacg
     2701 ctttatggtt gtcacatcaa catccagcac taaacccggc tgtatatgat gtggattaat
     2761 cttataggta aactcattaa accttctttc taagaagtta atacgctcat caagaataac
     2821 tcgttcaatt ggattctgaa aaccatgcat tttgtgtaac atctcgcgca ggtatttaag
     2881 tttattttta agattgtgaa tcctatcttc atatgttctg ttgttctttt caacaaatga
     2941 attttcatta tacatttcac caatttcatt ccataatact gaattaggat ctgcatcctc
     3001 atcatgttta ttccttctcc aatctcttga cataagtctg tttgataaat cgtcaaaatc
     3061 acggagtgaa cggaatctct tctttgattc ataatgagca tgagcaacat cccataccct
     3121 gagtatttca cgggtaaagg aatccatctg tctgtcaaac tccttcttag cttcacgcaa
     3181 ctgattctga tcataatatg ctaatctaat tgcatagcgc tcatctggca atacagactt
     3241 atctagttcc tcatattctc ggatctgaca tacacgtgca ttcttcaagt tatcgcaaac
     3301 ctggtagcct aactttgatg tatcaagaat tgaagtaata gcattgacgg cagtgttata
     3361 tcctcgattt cggatattct cttcgtcaat gatgtattta atattttcac gaatatttag
     3421 tggatcatat tcttcaacat caatctctgc acgtaatcct tctattttat caaggaactt
     3481 ctttgcaagg attgtatacc gtttagattt ttcatcttct ttttcatcat ctgtgaagtt
     3541 ttcaattctc ttaatcttct caaacataac ttccgtaccg gtaagctcgc tcttaccctg
     3601 atctataagt tcttccttga ggatttgaat ctgcttatct atcaattcat ggatatgttt
     3661 ttgaattgca tctttcaata atgactcaac agttacctga tagtgataaa tttggctgat
     3721 tagctcacta tcaagaatat ttaccgagag cttaacatca tttacatatt ttggccttaa
     3781 ctcattatcc ttaaatacgc atttgataat ggcataagca ttttcccctc ggataaatgc
     3841 accaacatct gtcttctgtc ttaatattgc gttggtttca ttttcaaggt cattcaagcc
     3901 tcgctggata tgtccctgca ggtggccgta catatttacg atagattttt caatttcacc
     3961 tgtattaaac ttatccacac ccccgatttt atcaagaagt tccataatct cgcgtggtgt
     4021 atagcgagct aatccctttg cttcttctct gtctacaaag tcacgaactt ttttgataaa
     4081 ttcatcttcc atggtaaccg tataacgatt aaacatatta acataagact ggttaacata
     4141 attatatagc ttctctttta aaccacccat cacatctaac ttcttcaata cttcttcggg
     4201 tagtttagtc tgaacatgat taacaagctt atcgacagct tcatcaacca taagccttgc
     4261 ttctgcaacc ttatcacggc cttcctgtgc taatgaattt cgtgaaccga ccgcactggg
     4321 ttcagttgga tgaactttat tagggctttt tggaaactga gttattgaca tgtattgacc
     4381 tccttgattg aatacatttt tttcaataat gcattgtata taattttcta ctattgttta
     4441 catagtagtt ttaatccaaa acacctgtaa gtaatattta taatactatc actatgattg
     4501 tcaacgtttt atttaaattt tttaaaaaaa atttttaatt ctgaatacct cattaccctt
     4561 cataatactt aaatgtgtaa ttgttaattt taaatatatc accatcattc agaatattcc
     4621 ctgcatccct attaacaaat tgtacaacat tttcaggcgc caacaactga cacttcacaa
     4681 ccttatcttt tctcactgct ttaagcatta tcggttccct gatagcaaga tcatgaattc
     4741 gtatcatatt atgggtatcg ctaccaatgg taatagttct tgaatttttt gctgtaagat
     4801 ttgctttctc tattgaaggt tcaagcaatt cattgttcca gtattctaaa gcacccttca
     4861 ctttaagctg tgcagctctc cttaacacca tgaacagaat aattaaaaga actatcgcca
     4921 ctattataat aaaaagcgga tgcatataca atgggcgtga atgtatttct tttatctttg
     4981 ccattgtatc aatattcgtt ttaagatcct tgacaataat tttatctact gactcctgag
     5041 catctatcac cttggtatat ggtgaaatgc tttcttttac ttctttttga attttttcca
     5101 aacgactatc cttctggatt tgccccaggt taacaaagaa aacaaaccac tcattccctg
     5161 aatactgcct ggctatattt ttaatatcgt aatgatactt acgagcatct ggtggaggat
     5221 catctattgc atcagtaatg ataataatga cctgctctct gtcaggatcc tccatctgta
     5281 attgttttgc cttatcaaat gcagcagtaa tcatcgccat ggtatacgtc catttaccac
     5341 ttgcttccac catcgagata tatttgttca aaatatcttt attgcttttg tgttttatat
     5401 aaacggtggg atataatttt acatcagtgc taaatgtaat caaggtaaag ctatcatttt
     5461 catcgagctg ttcaataaat tttggtaaac tttgttttac cagtggcata atatttttac
     5521 caccatatcc cgccattgat aatgacgtat caataacaag aatcaaatcc ctggattttg
     5581 catacccatc agcgtaacat aatccagcaa atattatgat agctataatc aatcgaaaat
     5641 attgaaccgt aactttcaag gcgaactcct tatagtattg tatatttttt acattcagag
     5701 gcagccacaa atctttctaa taatcagtct agtataaata acaaaaaata tttaagtcaa
     5761 gtaaataatt taaatttttt tattatttta aactatagaa tatgtattaa tactctctgt
     5821 aacgacgagt ttcttttaca taatgtcgta aatccagtga acgttttaca tcaacatcaa
     5881 gatattcatt aatcaggata aaaaaccaca gcaaggtatc ttcctcagtt tcaagtgcaa
     5941 caaattttcg tttttcacgc caatccttaa taattatccc tttcccaatg atcatcatga
     6001 tatcaaattc aaacatacta tcaacactat gatcaacata ataatcaatg acatcatttt
     6061 ttagttgtgg cacatctata tcgcttgctt gaacaataac aaatgctgga ataataatat
     6121 cctgatcaag ttcagcatat ttattcaatt ttttagttaa gattacgtta cttaaaacct
     6181 ctgataatcc ttgtttatca atgtgttttg ttatatgaaa ggtagcatac actaattcgg
     6241 atggaatatg cccattcatc atttcttcaa cgatagccca ttttttataa tatgctatat
     6301 aatcaactgt tttaggtaca tatccgctag catcaacagg ttttttatca gatacaataa
     6361 actcattggg aactacatcc tgtaacagtt tcataatttt cttaccatca aattcttctc
     6421 tgacttgatt ttttttggat gcctttttac catctttaga agattgtaat cctgatatgg
     6481 tattctgtat acttgcgaag tactctttta acatttaaat tctccacctg aaattaactt
     6541 tttcttaaaa taactaatac tgaacattat agcactgctc atatgcaaca aatatatatt
     6601 ccactcaaat aaaatcatca ttgattaagt ataatattgc caatacataa aatcaatatt
     6661 atttcacatt gtttatagta attgctatta ttggctattg ttgaccggat tacaatttag
     6721 ttaaaattct gccacattaa catcattaaa atcctgtgaa actgaaccca ccacttcttc
     6781 atcttcttca agtcctaaat cattttcgtt ggtgttattc ttttgaggtt gcggtttttg
     6841 tttcaattct tcttcaatgt atccttcgct ttcagctttt ttactttcaa taatattttg
     6901 taattcagca gtactcatgt ttttccactc tttggcaact gcaacatatt ttccattggg
     6961 gaattcgcga atatatcgtt gaaattcacg cgcagcaaga tcaaattttt tctgcataaa
     7021 ataagcatac ccttttttaa tctgtgcatc ctgatcttta tgataaaagt catttgtcaa
     7081 tactttatta aaataagcga tagctctatc aaactgtcgc attgcaaaat atgattcgcc
     7141 tatccagtat aatgcatttt ctgataattt atcaccacca aaatgtatca ataccctgtt
     7201 aaaaaatcct attgcatcat tgtaacgtcc actcctgaaa gcattaagac cacttttata
     7261 tgcctgtacc ttgtatgcat tttttacatc attggcatac tggcttaccg caccataata
     7321 ccgtaaaaag taatcatagt gattatatgc ttcagcatcc ttccctaaat gctttaatga
     7381 acgtgctctt cctaaaattg cttcttcatt ggtgctatcc tcatttatag caatatcaaa
     7441 catacgtttt gctttttgta tctcgccatt tttcagatac aaatgnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnncctat atagtaatag gcttcaattg ctgttttagt
     7621 atcaggatac cgtgcaatga tctttgtcaa aatggtaaca gcctgattaa tggattcttt
     7681 atcatttctc tcataaataa gcgttgcttc ggtaagtaac ttttttgcct cactctgagg
     7741 ttgtattacc aggacataat atattattaa caatatccca ataacaatca ataatgcccc
     7801 tgctaatgtc tttgttttca tagttacctc gttactctat atcataaatt tattgcgacc
     7861 gtgctattgc aaaatcttgc cagaactttg catcttcagg aaaatatgtt gctacatctt
     7921 tttgcattaa atagtgtaac gcttttttat actgctttaa ttcaatgtaa cttctgccaa
     7981 tatagagatt ggcttttgca atctcaatac tattcttgga ttgttcactg tatttgctta
     8041 acgtatcaat acaggtttca tattttcctt tataaaaggt tatcgcaatt attttgttaa
     8101 tatctgcggt agctgataat ttttgctttt ccagttgata gggtttggct tcttcctttt
     8161 gagattcagg caattctgat ttgccagtct tttctttttc aggatcttgt aaatccagtt
     8221 gtgcttttgc catttttgta acctttactg actctgcagt gcaattaaca ccctttataa
     8281 ttttcaattc attatcatac gtttccggaa ccagagcata ataatatgaa ccagcgggga
     8341 tgttgctatc aatatattca ttatttgtaa tattgataac atcaacaaga tatgcattgt
     8401 ttaaatcctg tactgagaag ggagcattac gcaatctgaa aagtttaaaa tacctgttcc
     8461 ctgatgaacc tgtataagcc catgatattt ttaccaatcc attctgttca atagcttcta
     8521 atagttttag gctaatagga tgaccaaccg taataggaac agtagtataa ttcatatcag
     8581 gttttaaggt agtatcaata gaactatctt caagtattgc taatactgca aaatagtaag
     8641 tacccggaat tggatgagta tctatatagt gggatcgttt tgcatcaaca gttgcaacct
     8701 ttgttgctaa tgccaaatcc tgagcatcag tgataggctc agtatgccta tatataacaa
     8761 aatttttaac atcaatatct ggtttattcc agctcacttt aagatagtca ttatcaagtg
     8821 tagccttgat acccgtcaca aatacatcac gccgtaaaat aacagaaaca ggtgcagaag
     8881 tatagctctg atcaggtatt agttgaatat cttcattgcc ttccatatct ttaactgtaa
     8941 ctgcataata gtatttccca gtttgtgcga tagttctgtc tatatagctt tctacatcag
     9001 attgaatctc ccctaccaat cgtgcatttt ttatttttcc cggactattt aaaggttcag
     9061 ttgccctata aacattatag acaatatttg gttgttgaat acctttccat gttaacaaca
     9121 cctgagtttt attaacaata cgtgcataga gcaaggtaac atgatcatac aaacgctttg
     9181 ctggtaattc agggatcttc tttttaatta ctattggcgc actggtgtaa tttacatcag
     9241 ggtatagttc aatatctttt tcaacaatct tatcccttgc taaaacaaca taataatatt
     9301 gacctggcgg tagatttgaa tcgatagcaa cgggttctgc tcctgctaaa actactttta
     9361 ctgttgtagc ctgtaatgcc acctctggtg tggaaggaac ccgggttgtc ctnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcttatta cactatttcg gaaatattta
     9541 tacaacactt gatgtattat taaaaaatta aaaatgctaa ataaaaaaat tagggctcct
     9601 aagaaaaata cttgacctgt taagctatat ctgttaacaa taatagccat ttgaaagtac
     9661 tattggttat gcagtaatgg caaaaatgtt ttatataact ggtgccattt tgtaacacga
     9721 gaataaaatt aatcatgtat gttttctgct atatttcact actgaaaata tttactattt
     9781 ttttataaat tttattgtaa aataatagct atggataaac aagaaaaaat acacaaaaag
     9841 aaaattatta ttctggaagc tcttaaacgt ttgttgcaaa atgatgttta ttcgcgtatc
     9901 acggtgcagg ctgttgctga tgaagcaggt ttttcaaagg ggggattgtt acattatttt
     9961 cctactaaag aagaaatgta tattgaattt atggaatacc tttttaatga aattaataac
    10021 gaccataaga atgtgctgaa aggtaatctg cattcaaaag ctaaagcaag tatttccgcc
    10081 atgtatggtg tcgaacgttt ttttcttgat ccggctaccg caaagatatt aatcaacctg
    10141 atgttatatg cttatgaaga tgaaaaaata atggtacgtc ttaaagaatt tatacgccgt
    10201 catctggaat tatataaaac cattataaat gaagcacgtg aagatcagcc acaacgcaga
    10261 aaaactgact ttgatgctga atttcatgct cgtattgcac agaccatagt tatgtctgca
    10321 gggctttttg aatctattga cccgctcccc attgacaact taaactttat gcgttacatt
    10381 atctctcttt tcagaggata aaacattatt gaatatttag tttgtcataa accatgaata
    10441 atagtgcctc agtatcaatg taaataatcc taaaaactaa taagtgtatc gttatttact
    10501 cacgatcctt tctttatgta acgtactggt accatggttt catacctttt catatttcaa
    10561 cggaaggcta tgaccatgta atattcgttg cacgatgcta aattcatggc acaatcaaat
    10621 aatcctatta atgtataaat tgtatagtat atttttcatg acatataatg ctgaaaagtc
    10681 atttattatt tgacagtttt acttat
//
LOCUS       DAOOJH010000081        10694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5565_length_10694_cov_9.701006, whole genome shotgun sequence.
ACCESSION   DAOOJH010000081 DAOOJH010000000
VERSION     DAOOJH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5565_length_10694_cov_9.701006"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..701
                     /locus_tag="PLJ75_09770"
     CDS             570..701
                     /locus_tag="PLJ75_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05540.1"
                     /translation="MTCDKCGAEINRLNHYYIPGDKNTKGKRYCIRCAKEEKIVTLI"
     gene            808..1911
                     /locus_tag="PLJ75_09775"
     CDS             808..1911
                     /locus_tag="PLJ75_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPD05541.1"
                     /translation="MENDQISILIVDDEEKICNRLATVLQREGYCTTATTESQQAINY
                     LSQQPFDIIITDLNMPQHSGFQIMEYIQQAGINILIIVITGYASVDAAIKSIKLGAYD
                     FIQKPFDIETLKLTIKRAAITIKLRRENERYVEELKKLNTLKNEFISIVSHDLRSPLA
                     TIGAYVKYLTKKEDFPEKYMRYLYIIGDIAENLYNLVNELLDVSKIERGILHLNYEEV
                     DINTVITSTIETFSTLAKEKNNEIIYTQKPMQPIIRIDRIKIIQVLNNLLSNAIKFTE
                     NGTITIKAISHENGISIVIQDTGIGIDDELKKSILDTYTLLHTNGTRGEIGTGLGLTI
                     CKRFVELHGGSISVESEKDKGSIFTIFLPYTIT"
     gene            1929..3026
                     /locus_tag="PLJ75_09780"
     CDS             1929..3026
                     /locus_tag="PLJ75_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05542.1"
                     /translation="MAKQFLKLSFSKRYPLFIIVTISSCCIAVIIVTINVISSMYTTS
                     VDYSKQLSIQHIESMSMVLHKLISETIAKPDTTHDTNTIPDKKKLSQLVEQINAIATF
                     YIAKPTDNDRNYIVVETIKAAPLQGKIITDHSANFIMRKGLLRVTVNPSIKDHPYSYI
                     ESYMPLKYNNKNYIIIIHWLVPHIGILQNKHAADLRSLIQIIAIIILVTVFIITFLSI
                     IHFMRTRSLLQELSHSIQSIANGNMDMMLNDSIDNDLKTIAVSFNSLAQEFKNKDEAL
                     RKIKEETFTDIFKQGVQRLKENQFDEAIACFITVTIFKPNSFASYFNLGVCCAKKGEL
                     NKSVEYFTQAQEINPDNQLTRCYIEKIQGLQ"
     gene            3056..4840
                     /gene="uvrC"
                     /locus_tag="PLJ75_09785"
     CDS             3056..4840
                     /gene="uvrC"
                     /locus_tag="PLJ75_09785"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPD05543.1"
                     /translation="MQKVKNLPPKPGVYLFSDAKNEIIYIGKASSLKKRVSSYFKMHV
                     DAKTNVLVSSIHDIDYIITQSEIEALLLESSLIKKHKPKYNIQQKDDKRFPYIAITLH
                     EQFPRIIFTRKIVYNGDKYYGPFTDAQAARKTVSLINKTFQLKTCTKEIPLKPGQRPC
                     LNQQIGRCQGVCTGIVSQAEYRNVIHAVMQFLDGNIDPVVENLTALMNTYSNTMRFEI
                     AAQIRDMIYDIQTISQEQHVVTPLGQNQDYIGLTAHQDEAFILVFQFRHGSLIGKRIF
                     VFTNAEYTRTSTFIAQFIVQYYASNEPPQSIIIPEATDDKQILEQYTSEKAAQPVTIA
                     LPRTSHEHAVMKLIQKNLDLLVAQKIAPDQKKALFELQQVLALPALPVTIECFDISNI
                     QGRFAVASMVHFTDGMPNKKEYRRYRIRAYLQPNDPGMIHEVVGRRIQHCINEKLPLP
                     DLLVIDGGKAQLLRALEVLNTFECSIPVIALAKRFEEIYTPHSYPLRLPVTSPALKLL
                     QTIRDEAHRFAITYHRNLRDKNLTTSILDEIPGIGTQYKKKLLQNFATIQGIKQSDID
                     TISSQAKIPKSVAQKVYHFFNAQSEEKQ"
     gene            complement(4886..5512)
                     /locus_tag="PLJ75_09790"
     CDS             complement(4886..5512)
                     /locus_tag="PLJ75_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HPD05544.1"
                     /translation="MSDASFIPTVFFTLFFVVDPLGLIPVFVAYLAPCDAAYRKKVIV
                     KATAISIGVSLFFIFSAHVFLRFIGITPGSFMIAGGILLFIIAMDMLLARPRRTQQFD
                     DKEEYTMDTDIAVFPLAIPFLCGPGNIAALLMFSSQAQGDIVKLGIISIISIATFLIA
                     MLVMAIALPLQTLLGRTGISVISKLMGLILSAMAVQFIYNGLVQMGVR"
     gene            complement(5515..7101)
                     /gene="serA"
                     /locus_tag="PLJ75_09795"
     CDS             complement(5515..7101)
                     /gene="serA"
                     /locus_tag="PLJ75_09795"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPD05545.1"
                     /translation="MRKKILITDEIAPEGLKLLQSYHDFDIVYKPGLKADDLKKEITN
                     AQALIIRSATHVTKDIIDAGAKLEIIARAGIGVDNVDVDSATAKGIIVTNAPGGNTVS
                     TAEFAITLICALARHIPQAYTSMKQQKWERQKFEGTELRRKTIGIIGLGRVGSELAKL
                     CKGLSMNVVGFDPYVAKEKLTALDIDIVDLDTIWKKADFISVHTPLTNETNNLINADV
                     IKKLNQGVFIINCARGGIVNEQDLYNALKEKRIAGAALDVYTQEPPDNLPPFHELNNV
                     ILTPHLGAYTYEAQVSVAIEAAQTIVDYFTRNIVQNSVNFASVDMNQYNFMKPFINCM
                     ERMGRFQAKMLDGKISSVQIYYCGEFGNFSKDPLTAAYLKGLLSPFIDRPLNYVNATF
                     IASERGIKVHSGESTEAGDWAHLIQVKAEGGALKSELWGTVIAKQPWVVRFDEYLIDF
                     IPSGRMLVMHNNDVPNVIGVIGTFLGQHHINIANLHLARTRRGGKALVILEIDEDVPH
                     DVLTMLSGLPEILDVRYVVV"
     gene            complement(7431..7658)
                     /locus_tag="PLJ75_09800"
                     /pseudo
     CDS             complement(7431..7658)
                     /locus_tag="PLJ75_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7731..9293
                     /locus_tag="PLJ75_09805"
     CDS             7731..9293
                     /locus_tag="PLJ75_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05546.1"
                     /translation="MIIPKIKNIIFLEREEFPASYNGELNYQCSKVIFFASLVCIFAW
                     LSYVGVDSQICPDQPLIPLLRYGLTAVSLIIFILQFFHFFKRHSMALLAILGLYLEAA
                     TGIITGLAKGDTVYMGGYFFVLMIPLVAPMNKFILWSEILFSVALFFVIGLSLGMEFV
                     TVRDQYTLNDMIVVVLFTLGFIYILDRLRYASWQKSQQIEEQRIHIQFEKERAESIVA
                     EAKTVVEHVRKASMIINNFSGDITAIINEHSTIFTKSRDIGTNIIQSFQVLREATFKE
                     IENNREINDLTRGLGMDLSNAAETGKEASTEAKKIQELSDDCNRKLQEAHKTIERLRD
                     ESKMIEEISQTINEIADQTNLLSLNASIESARAGEHGRGFAVVADEISKLADKSQSSA
                     KEISGIIGRSVERILSASVQIDEASGALTEIIGFLETNRSFFIKFQQLVSSQDKNIHT
                     VIDHLENSLEYTGYISELTEKNSNAIAQSQEALGQIHHFYDSLTEISLTFKHLSGELT
                     GHVDNLQKTLGE"
     gene            complement(9565..10476)
                     /locus_tag="PLJ75_09810"
     CDS             complement(9565..10476)
                     /locus_tag="PLJ75_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HPD05547.1"
                     /translation="MTNLTHIIEKYFLVWIVVFSLCGYHFPALFKGLAAYIPLFLGII
                     MFGMGMTLEFTDFIHIAKIPGALIAGVIGQFIIMPFVAYLLCLVFQLPPMLAIGVILV
                     GTCPGGTASNVVTYLSKGDVALSVALTITTTLVSPLATPLLTYLYAGTWIHVPVIPML
                     VSILQVIIIPIILGITIRSVFKEKIKTVITILPSVSVVAIVTIVAGITAANALALKTV
                     GLLVIGVVMVHNVLGLIMGYLASWLFGFDEKIRRTIAIEVGMQNSGLAVSLAVIHFEA
                     ISALPGALFSIWHNISGSLIAWWWRRH"
     gene            complement(10617..10688)
                     /locus_tag="PLJ75_09815"
     tRNA            complement(10617..10688)
                     /locus_tag="PLJ75_09815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10653..10655),aa:Arg,seq:ccg)
BASE COUNT         3499 a         2208 c         1919 g         3068 t
ORIGIN      
        1 gcagaatcta taacagtttt aaaccgtttt accacctctt tatcaacggc atccatagct
       61 aatttattca ttgctgtaca caattttaat agattagtct ttcttttacc ctttgatggt
      121 gattttttta cattagatct cgatggcatt atacaataac cccctaaata atcagcaatg
      181 ctaaaaataa ttgtatataa tgcaaacata attttcaaaa aaaaaattcc atacactatt
      241 atatcagact gcatataagc atgattacca ataccatagt aacacataac agaataatct
      301 aaaaaatttt tgatacccat atgacaacaa aacaatagca caacaccttg cccggctgtt
      361 gatactataa actggtttta taactgcttg taaaaaaata taacaaaaag acttttcttt
      421 accgacaatt atcatacatg aacctctgat aattatttta cggatgttcc atggcgggcg
      481 agcattggaa aaattatttg ttgtttttcc agataatgaa aaattaattt ttagagtgtc
      541 tataaacttt ttatacagga gtatatcata tgacatgtga taaatgcggc gcagagataa
      601 acaggcttaa ccactactac atacctggcg ataaaaatac caaaggtaaa cgttactgca
      661 tccgatgcgc taaagaagaa aaaatagtta cacttattta gataagcact aaaagtttta
      721 ctaaaacatg acatttatgg tacagggcca ttgcagcagt aacaattaac ttgacatggc
      781 tatcgatgta atgacataat cctatgtatg gaaaatgatc aaatttctat ccttattgtt
      841 gatgatgaag aaaagatatg caatcgctta gcaaccgtat tgcagcggga aggctattgc
      901 actacagcta caacagaaag ccagcaggca atcaactatc tttcacaaca accatttgat
      961 ataattatta ctgatttgaa tatgccacaa catagcggtt ttcagataat ggagtacatt
     1021 cagcaggctg gtattaatat acttattatt gttataaccg gctatgcatc ggttgacgcc
     1081 gctataaaat ccattaaatt gggtgcttac gattttattc aaaaaccttt tgatattgaa
     1141 acgctgaagt taactataaa gcgtgcagct attactataa agctgagaag ggaaaatgaa
     1201 cggtatgtag aggagcttaa aaaactcaac accttgaaaa atgaatttat ctccattgtt
     1261 tcccatgacc ttcgctcacc actggcaact attggtgctt atgtaaaata ccttactaaa
     1321 aaagaagatt tccctgaaaa atatatgcgg tatttgtata tcattggaga tattgcggaa
     1381 aatttatata atctggtcaa tgaactactg gatgtctcca aaatagaacg tggtatttta
     1441 catttaaact atgaagaggt tgatatcaat acagttatta catcaacaat cgaaaccttt
     1501 tcaacactgg caaaagaaaa aaacaatgaa atcatataca cacaaaaacc catgcagcct
     1561 ataataagga ttgacaggat aaagataatt caggttctca ataacctttt gagcaatgca
     1621 atcaagttta cggaaaatgg caccataacc ataaaggcaa tatcacatga aaatggtata
     1681 tcaattgtta tacaggatac aggtattggc atagatgatg agcttaaaaa aagcatattg
     1741 gacacctata ctctgctaca taccaatgga acacgtggcg aaataggaac aggactggga
     1801 ttaactatat gcaaacgttt tgttgaactt catggaggtt ccatctcagt tgaaagtgaa
     1861 aaagataaag gcagtatatt tactattttt cttccttata caattacatg accttgctga
     1921 ggctgaccat ggcaaagcaa tttcttaaac ttagtttttc aaaacgatat ccgcttttta
     1981 tcattgtcac catctcttca tgctgtattg ctgtaataat tgtaactatc aacgtaatct
     2041 cttctatgta taccacatca gttgactatt caaaacaact atccatccag catatagagt
     2101 ctatgagcat ggtcctgcac aaactgatat cagaaactat cgctaaacct gatactacgc
     2161 atgatacaaa tactatacca gataaaaaaa aactatctca acttgttgaa caaatcaatg
     2221 caatagctac attttatata gcaaaaccaa ctgataatga cagaaactat atcgtggttg
     2281 aaactataaa ggcagcacca ttacagggta aaatcattac tgatcattca gccaatttca
     2341 ttatgcgaaa agggttgcta agagttaccg ttaatccatc aatcaaagac cacccttaca
     2401 gctatattga aagctatatg ccgctcaagt ataacaacaa gaattatatc attattattc
     2461 actggctggt tccccatata ggcatattgc agaacaaaca cgctgccgac ttacgatccc
     2521 ttatccagat aattgcaatc atcattcttg tcacagtttt cattattact tttttatcaa
     2581 taattcattt tatgcgcaca cggtcccttt tacaggaact atcgcacagc attcaaagta
     2641 ttgcaaatgg caatatggac atgatgttaa atgacagcat tgacaatgat ctaaagacta
     2701 ttgctgtttc ttttaattca cttgcacagg aatttaaaaa taaggatgaa gccttaagaa
     2761 aaataaaaga agaaacattt acagatatct ttaaacaagg ggtgcagcgt ttaaaagaaa
     2821 atcaatttga tgaagccata gcatgtttta tcacagtaac tatatttaaa ccaaacagct
     2881 tcgcaagcta ctttaattta ggtgtgtgtt gtgcaaaaaa aggtgaactt aataaatcag
     2941 tagaatactt tacacaagcg caagaaatta atcctgacaa tcagctaacc aggtgctata
     3001 ttgaaaaaat acaaggcttg caataaggta tagcagcaca atgtatgagc agttagtgca
     3061 aaaagttaaa aacctgcccc caaagccggg ggtgtatctt tttagtgatg caaaaaatga
     3121 aattatttat attggtaaag cgtcatcgtt aaaaaaaaga gtttcttcat atttcaagat
     3181 gcacgttgac gcaaagacaa atgtgcttgt ttccagcatc cacgatattg attatattat
     3241 aacacaaagc gagattgaag cactgctttt agaaagctca cttataaaaa aacataagcc
     3301 aaaatataat attcagcaaa aagacgataa gcgatttcct tacattgcta tcacgctgca
     3361 tgaacaattt ccacgtatta tatttacccg taaaatagta tataatggcg ataaatatta
     3421 tggaccattc accgatgctc aggctgcacg gaaaacggtt tcgcttatca ataaaacatt
     3481 tcagttgaaa acatgtacca aagagatacc attaaaacca ggacagcgtc catgcctcaa
     3541 tcagcagatt ggcagatgcc agggggtatg cacgggtatt gtttctcaag ctgaatatcg
     3601 caatgtcata cacgctgtga tgcaattttt agatgggaat attgatccgg tggttgaaaa
     3661 cctcactgct ctcatgaata catattcaaa caccatgcgc tttgagattg cagcacagat
     3721 ccgtgatatg atatacgata tccagacgat atcacaagaa cagcatgtgg taactcccct
     3781 ggggcaaaac caggattaca ttggtttaac cgcacatcag gatgaagcat ttattcttgt
     3841 atttcagttc agacatggaa gtcttattgg gaagcgtata tttgtcttta ccaatgcaga
     3901 gtataccaga acatcaactt tcattgctca attcattgta cagtactatg ccagcaatga
     3961 acctccgcaa tcaattatta tccccgaagc aaccgatgat aaacaaatcc ttgaacagta
     4021 tacttcagaa aaagcagcgc agccggtaac cattgcatta ccccgaacca gtcatgaaca
     4081 tgcggttatg aagctaatac aaaaaaattt agatcttctc gttgctcaaa agattgctcc
     4141 tgaccagaaa aaagccctct ttgagctgca gcaggtgctt gcacttcctg cactgcctgt
     4201 aacgatagaa tgttttgata tatcaaatat tcagggtaga tttgccgttg catccatggt
     4261 gcattttacc gatggcatgc ccaataaaaa agagtacaga cgataccgta ttcgtgctta
     4321 cttgcagccc aatgaccccg gcatgattca tgaggttgta ggcaggagga tacagcattg
     4381 cataaacgaa aaactgccgt tgcctgattt gcttgttatt gatggtggta aagctcagct
     4441 tttacgagcc cttgaggtac tcaacacatt tgaatgttct ataccggtga ttgcccttgc
     4501 aaaacgtttt gaagagatat atactccaca cagctatccc ctgcgccttc ctgttacatc
     4561 acccgcatta aaactattgc aaacaatacg cgatgaagct caccgctttg ccatcaccta
     4621 tcatcgcaat cttcgcgata aaaaccttac cacatcaatt ctggatgaaa taccgggcat
     4681 tggtacgcag tacaaaaaaa agttgctcca gaactttgca actatccagg gtataaaaca
     4741 gtcggatata gacaccattt catcgcaggc aaagattcca aaaagtgtag cccaaaaggt
     4801 atatcatttt tttaatgcgc agagtgagga gaagcaataa caggtatttt tattttatgg
     4861 tgcgatagtt actatatatc aataactatc gtacacccat ctgtaccagc ccgttataaa
     4921 taaattgtac cgccatagcc gacaggataa gtcccatcaa cttgctgatt accgagatac
     4981 cggtgcgtcc cagcagtgtc tgcaatggta aagcgatagc cattaccagc atcgctatta
     5041 agaaagtagc tatagaaatt attgaaataa tgccaagctt tactatgtcc ccctgtgctt
     5101 gagagctgaa catcaaaaga gccgcaatat tgccgggacc gcataaaaaa ggaatggcaa
     5161 gcggaaaaac cgcaatgtcg gtatccatag tatattcttc tttatcatca aactgctggg
     5221 tgcggcgagg acgtgcaagc agcatatcca tagcaatgat aaacaacagt attcccccgg
     5281 ctatcataaa tgaaccgggt gtaataccta taaagcgcaa gaatacatgt gcactgaata
     5341 tgaaaaataa cgatacacca atagatattg ccgttgcctt gacgatgact tttttacgat
     5401 atgcagcatc acacggtgcc agataagcaa caaaaactgg aattaatccc agagggtcca
     5461 ccacaaaaaa taaagtaaaa aatactgtag ggataaagct tgcatcactc atggttatac
     5521 cacaacatat cgcacatcca atatttcagg cagccctgat aacatggtca gcacatcatg
     5581 cggcacgtcc tcatctattt ctaaaataac cagtgcttta ccgccgcgcc gcgtccgtgc
     5641 cagatgaagg tttgcaatat tgatatggtg ctgccccaga aatgttccaa ttactccaat
     5701 aacattgggg acatcattat tgtgcatcac aagcattctg cctgacggaa taaaatcaat
     5761 gaggtattca tcaaaacgga ccacccatgg ttgctttgca attaccgtcc cccaaagctc
     5821 acttttcaat gctccgcctt ctgctttcac ctgtatgaga tgtgcccaat cacctgcttc
     5881 cgtactttct ccgctatgca cctttatacc ccgctctgat gcaataaacg tagcattaac
     5941 ataatttaaa ggcctatcaa taaagggcga tagtaatcct ttaaggtaag ctgccgttaa
     6001 agggtccttg ctgaaattac caaattcacc gcagtagtat atctgtacag aagaaatttt
     6061 gccgtctaac atttttgcct gaaacctgcc catgcgctcc atacaattaa taaacggttt
     6121 cataaaattg tactgattca tatcaaccga tgcaaagttt acactatttt gcacaatatt
     6181 tctggtaaag taatcaacaa ttgtttgggc agcttctatt gctactgaaa cctgcgcttc
     6241 ataggtgtac gcccctaaat gcggtgtgag gattacattg ttaagttcat ggaatggcgg
     6301 caggttgtca ggcggctcct gtgtatagac atcaagcgca gctccagcaa tgcgtttttc
     6361 ttttaatgca ttatataaat cctgctcgtt cactattcct ccgcgagcac aattaatgat
     6421 gaacacccct tgattaagtt tttttataac atctgcatta atcaaattgt ttgtttcatt
     6481 ggttagtggt gtgtgcacac ttatgaaatc agcctttttc cagattgtat caagatcaac
     6541 tatatcaata tccagtgctg tcagcttctc ctttgctacg taaggatcaa atcccacaac
     6601 attcatgctc aatcccttgc ataatttagc aagctctgac cccacacgtc ccaatccaat
     6661 aatccctatg gtttttcttc tcagttctgt cccttcaaat ttttgtcttt cccatttttg
     6721 ctgtttcatt gaagtatacg cctgtggaat atgacgcgca agtgcacaga taagggtaat
     6781 tgcaaattca gcggttgaaa cggtgttccc gcccggtgca ttggtaacaa taataccttt
     6841 agccgttgca ctgtccacat caacattatc cacacctata ccggctcgtg ctattatctc
     6901 caattttgca ccagcatcaa tgatgtcctt tgtaacatgc gttgccgaac ggataattaa
     6961 agcctgtgca ttcgttatct ccttttttaa atcatcagct tttaatccag gtttatacac
     7021 tatatcaaaa tcatgatagc tctgtaaaag tttcaatcct tcaggtgcta tttcatcggt
     7081 tatcagtatc ttcttcctca taaaccctcc ctgtacaggt atcattatag tatataatag
     7141 agcagaagta atgaaatgtc aatcactaca gaggggtttt tactgccttt ttctgcacca
     7201 tacagcagtt caggcttacc attgatgaaa tttattacaa taagccatat gttgctttat
     7261 ttaagaataa aatcacgaaa ataaatttcc ttaattttac ctgttagtaa caacacattg
     7321 atatgagcct ttaattcttc tgacagattt actcattctt caatagtata aaggtggtca
     7381 tattctttat cagctataat cagcttacat attttttcaa tcttatgatt ctacaaattt
     7441 gcggcgtatt ttgcaggtgt tgttccaaaa aaacgtttat actccctatt aaattgtaaa
     7501 ggactttcgt agcctgcacg taaagcagca gcatacgcat tgagaccttc ctgaatcatc
     7561 agttgacggg ctttatggag ccgtacgctt ttaatatatt gtagcggtga tgcgttggtc
     7621 actgctttat agctggtatg aaatgctgat acacccatac aaaagattat tgtataatac
     7681 tatttgacaa aacttccatt atcactataa atggtagcat gcattatcta ttgataatac
     7741 caaaaatcaa aaacatcata tttcttgaac gtgaggaatt tcctgcttct tataatgggg
     7801 aactcaatta ccagtgcagt aaagtaatat tttttgcatc gctggtatgt atttttgcat
     7861 ggttaagcta tgttggtgtt gatagccaga tttgccccga ccaaccgctg atacctctcc
     7921 ttcgttacgg attaacggcg gtatcgctta tcatatttat tttgcagttt tttcattttt
     7981 ttaagcggca tagcatggct cttttagcaa ttcttggact ttatcttgag gctgcaacgg
     8041 gaatcatcac cggactggca aaaggcgaca ctgtgtacat gggaggttat ttcttcgtgc
     8101 ttatgattcc gctcgttgct ccaatgaata agttcatact gtggagtgag attctctttt
     8161 ccgttgcgct gttttttgtt atagggcttt cactggggat ggaatttgta actgttcgcg
     8221 accagtacac actcaacgac atgattgttg ttgttctttt cacactcggt ttcatttata
     8281 ttcttgacag gcttcgatat gcaagctggc aaaaatctca gcagattgaa gagcagcgta
     8341 tacatataca gtttgagaaa gaacgcgctg aaagcattgt agcagaagct aaaacagtgg
     8401 tggagcacgt ccgaaaagct tcaatgatca ttaacaattt ttcaggcgat attactgcca
     8461 taataaacga acattctact atttttacaa aaagccgcga tatcggcaca aacatcattc
     8521 aatcatttca agtgctcagg gaagcaacat ttaaggaaat cgaaaacaat cgcgaaatca
     8581 acgatctcac cagaggactt ggcatggacc tgtcaaacgc cgctgaaacc ggaaaggaag
     8641 caagtactga agccaaaaag atacaggaac tctcggacga ctgtaaccgt aaacttcagg
     8701 aagcccataa aacaattgaa cgcctccgtg acgaatctaa aatgattgag gaaatatcac
     8761 agaccatcaa cgaaatagca gaccagacaa acctgctttc tctcaatgca tcaatagagt
     8821 ctgcgcgtgc aggagaacac ggacgaggat ttgccgttgt tgccgatgaa atctctaaac
     8881 tggcggacaa aagccaatcg tcagctaaag aaatcagcgg cataataggc cggtcggtgg
     8941 aaagaatttt gagtgcatca gtgcagattg atgaggcttc cggtgcgctt accgaaataa
     9001 taggatttct tgaaacaaac aggtcattct tcatcaaatt tcaacaactg gtatcttcgc
     9061 aggataaaaa catacataca gtaattgatc atctggaaaa ttcgctggag tacaccggat
     9121 atatctcaga gcttacagaa aaaaattcca atgcaattgc acaatcccag gaagcgcttg
     9181 gccagatcca ccatttttac gatagtctta ctgagatatc cctgaccttc aagcaccttt
     9241 ctggagagct caccggtcat gttgacaact tgcagaaaac attaggtgaa taagtactct
     9301 atagataatc aatctcaaat tcattgcata aaatatgata atcattttca ttaatagcat
     9361 tcgccattaa aagaccttcg aaagcgatat tttcaatttt tgttattaca gagacaatta
     9421 ataacatatg ctgatttttg tatatccaac gcaccgataa agtagagatg tgctttttta
     9481 cctcagtctg gcttataata attttctata actcatcagc acctcgttaa cagacaaaat
     9541 tacagcaatt atgtgtaata tgaactaatg tcgtctccac caccacgcaa tgagcgagcc
     9601 cgaaatgttg tgccatatac tgaatagtgc acctggcagc gcactgattg cttcaaaatg
     9661 gataaccgcg agggacaccg caagtccgga attttgcata cccacctcaa tggcaatggt
     9721 tcgccttatc ttttcatcaa agccaaataa ccagcttgcc aggtaaccca taatcagtcc
     9781 cagcacattg tgcaccataa caacgcctat cacaagcaac ccaactgttt tcagggctaa
     9841 agcattcgcg gcagtaatac ccgctacaat agtaactatc gccacaaccg aaacagaggg
     9901 gagtatggtt atgacagttt ttattttttc tttgaacacg gagcggatgg tgattcccag
     9961 aataattggt ataataatga cctgcagtat ggaaaccagc atgggtataa ccggcacatg
    10021 tatccatgta cccgcataca ggtaggttaa cagcggtgta gctaacggtg aaacaagtgt
    10081 tgttgtaatt gtcagtgcaa ccgaaagtgc cacatcgcct tttgacaggt aggtgacaac
    10141 attggatgct gtgccgccgg ggcatgtgcc aacgagtata accccaattg caagcatcgg
    10201 aggcaattga aataccaggc acaacaggta tgcaacaaat ggcatgataa taaactgacc
    10261 tatcactccc gcaataagtg caccaggaat ttttgctatg tgtataaaat cagtaaattc
    10321 aagtgtcatg cccatcccaa acatgataat acctaaaaac aacggtatat atgccgcaag
    10381 ccctttgaat agagcaggga aatgatagcc acatagtgaa aaaaccacaa tccacactaa
    10441 aaaatatttt tcaatgatat gcgttaggtt tgtcatatat gtgttccggt tacttataaa
    10501 aaaggtatgg ggcgatagtt actgtttaac aatttatagc atttaataat tgcaaaggtg
    10561 taatgaactg cgtagtggtt acaccagcat ggaatgtaca gagcaggtaa aaaactcgct
    10621 ctccccggga attgaacccg gacacacggc tccggaggcc gacgctctat ccgttgagct
    10681 aggagagctg tata
//
LOCUS       DAOOJH010000082        10644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5605_length_10644_cov_7.983851, whole genome shotgun sequence.
ACCESSION   DAOOJH010000082 DAOOJH010000000
VERSION     DAOOJH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10644
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5605_length_10644_cov_7.983851"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..415)
                     /locus_tag="PLJ75_09820"
     CDS             complement(260..415)
                     /locus_tag="PLJ75_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05548.1"
                     /translation="MKKRWLDAAILQSEERMHRLSGFMSIKPLIKSSEKVLSEEKIKI
                     DLTNLIP"
     gene            complement(469..756)
                     /locus_tag="PLJ75_09825"
     CDS             complement(469..756)
                     /locus_tag="PLJ75_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05549.1"
                     /translation="MFRENRTHEMPELFSEKNMMPDTLKKRYEKSWAKGFYEDVFCRI
                     DETLFSVLYSDKVSRPNLPVNIYVSLEILKELFGLSDEKFLGRFYFEHLNA"
     gene            992..1207
                     /locus_tag="PLJ75_09830"
     CDS             992..1207
                     /locus_tag="PLJ75_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05550.1"
                     /translation="MADFIDFVAKEAGNREMGEKFIKVLKQGDAQELKKFFDSNDYTG
                     VSIDDCKTLINNRQTIINSVDTNLKDY"
     gene            1215..4508
                     /locus_tag="PLJ75_09835"
     CDS             1215..4508
                     /locus_tag="PLJ75_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD05551.1"
                     /translation="MNFIKLIIFIISSLLLFSTVALSLDGNNNYVFSLSEEIMSLPKY
                     WYFRAEDREQYKEKNYDHSSWDIHAINQPWHTQKEFRNYTGNIWYRIVFNCDLCNSNN
                     YKGDFALLVPIHYRGAQFYLNGVLVKETRPFIKGISIPNLGKPDIIIFPHNLVTKENN
                     VIAIRISSFDYNSGFEGMIKIGTFPKISKVFVQNNLFYGLLIGINLFLSLYFILLFYY
                     RNKERFYLYFAGMAFSFALWTTGYIGIIFWIIDNRIAYLILAYCGSVSIVIFAILFIH
                     SFLSLRHNIFSKLLIAALVFFLIYCTIEIILTGLVVTYRMYLFRIFLLTCLIYMLYTT
                     ILCLYAIKIKRPHALKILAGIIVAYLFIFASVLTWLHIIDFTPSSIEPFMAMIFVFAF
                     VLASRYAQVFSELEKAHSKLLVLDKMKDDFLATTSHELKTPLHGIMGIVETIMDGSSG
                     PVTEKQIQNLALIRDEASHLNKMVIDILDFSKLRAGKVDLFLEHILVDQVAATIVSLL
                     TPEANKKGLSLTLKSSGPCAIVADRNRLRQIIINLVGNAIKFSDKGTITVNVESSKED
                     VTITVSDEGPGIDDSDRERIWNPFIQAESPDNRHYGGTGLGLPITKYLVELHDGSIHV
                     ESEKGKGTYFIVRLPKQPKTRGITKTKQIEVKNDSLVLPYEISPSSVEQDEELAKIPF
                     YKKAKILVVDDDEVNRKVIEGFCAIAGYELILAQDGPTALELMEKHNIDLVLLDLMLP
                     EMSGYEVCQKLRQSEKFVNIPIIIVTARDSASDMVRGFVTGANDYITKPFTRSELLIR
                     IENQLAIKQMLELERSIMHELNKEVEFNRGLYQRSIELKQMATQILAWERVIKEDMEV
                     AKVFQKRLMACPAHIPGLNFHFLYHPLAGLSGDVYDIIQLTPDIIRIFIADATGHGIT
                     ASLNTIKILSEYSSVKETMHASQDLLKFLNQRFIQLFKDYGIVFTCLIADIHLNEGKL
                     SLVSAGHPPACVISDGKVNFIKPHGPIIGLSSNYQYQPTTIPFKKKDTVLLYTDGLPE
                     FIYAQNKNFQDSSKNEYDKLKEIIEGHNTIESLENLCTSLMEGVSNRSNTFINDDITI
                     IAFQKDLGL"
     gene            4716..5315
                     /locus_tag="PLJ75_09840"
     CDS             4716..5315
                     /locus_tag="PLJ75_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05552.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKNHLQEDYFKACFVEILNRAK
                     QKYHFKLIAFCVMDNHIHLVIHTLEDGAPISRIMQYIKARFAELYNKITGRTGPFWNE
                     RYKDSIIQFAKDGFHYLLWLLWYLAYNPIRKRLCSDPIKYHYSSIKAYLQEEADVGVP
                     IDHHEYFVNLGETFTERVARFMQYEEYYRKKYSMILEWV"
     gene            complement(5346..7889)
                     /locus_tag="PLJ75_09845"
     CDS             complement(5346..7889)
                     /locus_tag="PLJ75_09845"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HPD05553.1"
                     /translation="MKLSFIKTIALITVSFCIVIFSTVSSFAKAKGLSVLCIYKSSEG
                     YTDDSNPLKWFFEKDITANGLQVKYYDFDKGFSSLSNLDDIRAIVTWYNGGVVASKDI
                     GINYAKFMIDAANKGIKIIIVNSYGAYGYKDGNETKWDLLPYIRPLFTKIGIYFQGFW
                     TNNPNNIKIIYKDSAMVEKDEKQDVTKSLHYQQIIPLREDVKTYLQLQRTDAPPQAGD
                     GKSSIIVISKTGAFALENYVVRGSKVMLNTSRFIHEALFYDDGYLNVGVIIGDIDRSN
                     VVNNNILYAFKYAKIRCDVYTKEEIKGLVANDLTEYEALLVATKTADAVPLKLLKGYV
                     ENGGKCIFLNYVEPDNELNTFIGIKEYIKKEEYFKQGFTLQPSLFLNGVNVPGDDIDC
                     NVRRAVLESDVKVIGTVNTLFDADKYPVVWERTIKKGKILYWNTNLLDKSKLTRGMIV
                     QSIYKIADVFATGMINVGLIMIDDFPAPWWNVAYKPYRIKYYSDLLQNEKDKFNRKKL
                     EEIIEKLKKLPEETDTLFISDVWFKDILAWQKQFGFAYSSFLIFNYNRDTKADETGFA
                     VRDFYLSQNGLSTKMGIAALENGFELGLHGYNHMSLTLTKAKEYDSVPWPDKKTMVQA
                     LTVAKNEWIALYGESALPFSYVAPHNIIDATGLQALGEVFPSIRVVSTLYVVKSGGVE
                     QEFDWSPDNRFYQIPRITSGYYFQDFDKFALYDAFQNFGIISHFIHPDDVFDEMRSKS
                     YEGWLWLKSNFEKEFGSLKQNYPWIRWMTVKDAFYEFVVYNSSTVNFKVKGNSIEIYT
                     PGGAGHNYYIRARIPKQYKQLINCNLVQYTPATGDIVVKTNKYKSVIAW"
     gene            complement(7870..10482)
                     /locus_tag="PLJ75_09850"
     CDS             complement(7870..10482)
                     /locus_tag="PLJ75_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05554.1"
                     /translation="MYEKQFLDLEIDNARIYKEIANFYLQNGNYNASREYYSNILKVQ
                     PLNVEANFAMGNINEFLGYWKDAKGNYEICINAQPDFEYARKAHYELQKQHAPSITGN
                     SIYFSDNIRTRYGIGLSGLYEPSQYFALQAGYELNKQTDSGGIATTGQLFYPSKGNVT
                     MHKAFAEIAVPFRFLYMRLFAHLGGTMYSGTVDYGNPLYDTSLSYMSLSYGAGFWWMP
                     PGTGVTVKVAYSHDDENDLTQSLKMKFRDAITYDMFEGILDINFSEYDFPLSKRLFWY
                     NALQYRMLSDTNTRLSSFNQLTVRVYRIPQWNMFFDIAGIYSYEDTKFNQYSSGDILL
                     VPYWAPQGVSYYGPMVKITQQVENLFGGRLLYSVFLQYQYDSLDQRIMMPGISLSQSW
                     DKLNLFAEYTYSNVTLPKSTADKTYISHDLKFGATGKFFSIYSPKGAGGKPVLFVSAN
                     PTLITPDGDGKDDFATLSLTAFDEKGIQQWWIEIYDEKEQKIQEYSLAGAVPGTFRWD
                     GTARNNVLAAEGKYYVQLTIQNSAGERFNSKKQEIVLSAAKRAIALTPSYPKFSPNDD
                     KIKDTVSFTIQATDKKNIASWFVSISNKNRIVKTIRGNDFIPYDVLWDGLDDNLKTVP
                     DGEYVAALTIKYDDGIVVKSPAATVTVVTKVAVDIKVEPNEITPLQTPLRITPSVDGD
                     VAQWQIRFMRDDGAVVKVINGAGMPPKVISWDGTDDKGEPAVYNMQLEAQMEITDDAG
                     NTAVSKKVPFMVGFLIKQEKGKRIVYMFNQDTIFKGRSDELTTHGVLILDQLAGKLAK
                     MGGFTRINIVAYTDAVGSKQSNMALSQKQAAVIAERLKNLAAEIKTVGAGAEVQYLKD
                     KPSKWDIRYEIELY"
BASE COUNT         3282 a         2103 c         1759 g         3500 t
ORIGIN      
        1 ttttcacaag aatggactga taactactaa atgaaactct aatttcacct gactcgtctt
       61 ttctaatatt gatttttggc aatcctttat tcatcattgt aatatcaaca tcagtctttt
      121 caaaaaaagt caatagaaat tacccataag agctcatccc gcttttagtg gaaattccat
      181 agcagtataa cattcatatc ctcaacaaat cttcctgtca attttaattt tttcttcgct
      241 caatactttt tctaattccc tatggtatta aatttgtcaa gtcaatttta attttttctt
      301 cgctcaatac tttttcacta ctttttatca gaggcttaat actcataaat ccactgagtc
      361 gatgcatccg ctcttcactc tgtaaaattg cagcatcaag ccacctcttc ttcatgtctc
      421 ctttggccca tcttttaacc ccagtttaaa accgttcaaa agaactattc aagcattcaa
      481 atgttcaaaa taaaacctgc ctaaaaactt ctcgtcactc agaccaaata attccttcaa
      541 tatctcaagc gatacatata tatttactgg cagattggga cgtgatacct tgtctgaata
      601 caatactgaa aatagtgtct cgtcaatcct gcaaaataca tcttcgtaaa acccttttgc
      661 ccagcttttc tcatatctct tctttaaagt atcaggcatc atattcttct cagaaaatag
      721 ttctggcatt tcatgtgttc tgttttctct aaacacaact atttctcctt aaaaaatttt
      781 gtgcccctat actatattgt tgacatcctg taaattaatt tatatgattt catccataaa
      841 attttattag taatactctt accctttttg caggggggtc atatatcaat ttcattattt
      901 tcaatttatt cagacactac aaaaatatgt tgaaatatta ttcatagtat tcattatatg
      961 tgtaattata gatatatttt tggaggtaag tatggcagat ttcattgatt ttgttgcaaa
     1021 agaagctgga aaccgtgaaa tgggggaaaa atttattaaa gtacttaagc aaggggatgc
     1081 ccaggaatta aaaaaattct ttgattccaa tgattacacc ggcgtatcaa ttgatgactg
     1141 taaaaccctt ataaacaata ggcagacaat tatcaattca gtagacacaa atcttaagga
     1201 ttattaatgc tggaatgaat tttatcaaat tgattatatt catcatatca tcgcttttac
     1261 tattcagtac tgtagcatta tctttagatg gcaataacaa ttatgtcttt tcactttcag
     1321 aagagataat gtcattacct aaatattggt attttagggc cgaggatcgg gaacaataca
     1381 aagaaaaaaa ttatgaccat tcatcatggg atattcatgc tatcaaccaa ccctggcata
     1441 cccaaaaaga attccgtaat tataccggaa atatttggta caggattgtt tttaactgtg
     1501 acctttgcaa tagcaataac tataagggtg actttgcttt attagttcca atacactatc
     1561 gtggagctca gttttattta aatggtgtat tggttaaaga aacacgtccc ttcattaagg
     1621 gaatatccat accaaacctt gggaagcccg atattataat tttcccacat aatttagtga
     1681 caaaggaaaa taatgtaatt gcaatacgga taagctcatt tgattataat tccggtttcg
     1741 aggggatgat aaaaattggg acatttccaa aaatatcaaa agtatttgtt caaaataatt
     1801 tattctacgg gcttttaatt gggattaatt tattcctatc gttatatttt attcttcttt
     1861 tttattacag aaacaaagaa cgattttatc tttactttgc cggaatggct ttttcatttg
     1921 cattatggac gaccggttat attggtatta ttttctggat aattgataat agaatagcct
     1981 atcttatatt agcatattgc ggttcggtaa gcattgttat ctttgcaatc ttattcatac
     2041 attccttttt atcgttacga cataacatat tctcgaaatt gcttattgct gcattggtat
     2101 ttttcctaat ttattgtacc atagaaataa ttcttaccgg gctggttgta acgtaccgaa
     2161 tgtatctatt caggattttt ctgctgacat gtcttattta tatgctctat acaacaatat
     2221 tatgcttgta tgcgattaag attaaaaggc ctcatgctct taaaatattg gcagggatta
     2281 ttgtagcata tttattcatc tttgcatctg ttttaacatg gcttcatata attgatttta
     2341 ctccttcatc tatagaacca tttatggcaa tgatttttgt ttttgccttc gtgcttgctt
     2401 cacggtatgc acaggtattt tccgaacttg aaaaagcgca ttcaaaactt cttgtccttg
     2461 ataaaatgaa agatgacttt cttgctacca ccagccacga actgaaaact ccgcttcatg
     2521 gaataatggg aattgttgaa acaattatgg atggttcatc cgggccagtg accgaaaaac
     2581 aaatacaaaa tctggctctg atacgggatg aggcatcaca cctcaacaaa atggtaattg
     2641 acatccttga cttcagtaag cttcgtgcag gaaaggtgga tctattcctt gagcatatct
     2701 tggttgatca ggttgcagca acaattgtat cacttttaac tcctgaagct aataaaaagg
     2761 gactatcgct tacattaaaa tcttctggac cctgtgcgat agttgctgat cgtaaccgcc
     2821 ttagacagat aataattaat cttgtgggaa atgcaattaa attttcagat aaaggcacta
     2881 ttaccgtaaa tgtagaatca tctaaagagg atgtaacaat tacggtgagt gatgaaggcc
     2941 cgggtatcga cgatagcgat agagaaagga tttggaatcc atttatacag gcagaaagcc
     3001 cggacaaccg tcattacgga ggtacaggac ttggacttcc aataacaaaa tatttagtgg
     3061 aacttcatga tgggtccatt catgttgaaa gcgaaaaagg taaagggaca tattttattg
     3121 ttagactccc aaaacagcct aaaacccgtg gtataacgaa aaccaagcaa attgaagtaa
     3181 aaaatgatag ccttgtgctt ccttatgaaa tttctccttc atctgttgaa caggatgaag
     3241 aacttgcaaa aattccattt tataaaaaag ctaaaatcct ggttgttgat gacgatgagg
     3301 ttaaccgcaa agtaattgag ggtttctgtg caatagcagg atatgaacta atactggccc
     3361 aggatggacc aacagctctt gaacttatgg aaaaacacaa cattgatctt gtacttctag
     3421 atctaatgct tccagaaatg agcgggtatg aggtatgtca gaaattacgc cagtcagaaa
     3481 aatttgtaaa catccccatt attatagtta ctgcgcgtga cagtgcatca gatatggtta
     3541 ggggttttgt taccggtgcc aacgattata tcacaaaacc atttacccgt agtgagttac
     3601 tcatcaggat tgagaatcaa ttagcaatta aacagatgct ggagcttgag cgttcaatta
     3661 tgcatgaatt aaataaagag gtcgaattta acagaggtct ttatcaaaga agtattgagt
     3721 taaagcagat ggcaacacag atacttgcat gggagcgagt aatcaaagaa gacatggaag
     3781 ttgccaaagt tttccagaaa cgtctcatgg catgccctgc acatatacca gggctcaatt
     3841 ttcatttttt gtatcatcca ttagcaggac tcagcggtga tgtgtatgat attattcaac
     3901 tcactccaga tatcatcagg atatttatag ctgatgccac aggtcacggg attactgctt
     3961 cactcaatac tataaaaatt ctttcagagt attcctctgt taaggaaaca atgcatgcat
     4021 ctcaggattt gctcaagttt ttaaaccagc gattcattca attattcaag gattacggaa
     4081 tagtttttac atgccttatt gctgatattc atctgaatga aggcaaactt tcattagttt
     4141 cagcaggtca tcctcctgca tgcgttatct cagatggtaa agttaacttt attaaacccc
     4201 atggaccaat cattgggctt tcttccaatt atcaatatca gcctacaaca atccctttta
     4261 aaaagaaaga tacagtgctt ctttatactg atgggctgcc tgaattcatt tatgctcaaa
     4321 ataaaaactt ccaagattct tcaaaaaatg agtatgataa gctaaaagaa ataattgaag
     4381 gtcataatac tatcgagtca ttagaaaatc tgtgtacatc gcttatggaa ggagtttcta
     4441 atagatctaa cacatttatc aatgacgaca taactattat tgcttttcaa aaagatttag
     4501 ggctctgacc cgattttctc cttttcctct gcatactccg cattctctgc gtgattattt
     4561 tttttaaaaa ttactatgct ctgacccctt tttttttcaa tttttttatc aaaaagcaca
     4621 aaattttttc taaatctttt taatttttta aacttcacgc gttgtataga gtaaaagcgt
     4681 aatgtaaata ttactacata cacggaggaa tacctatggc acggaaattg cgtgtacagc
     4741 taccaggttt aacgtaccac atcacttccc gctgtattga gtggaaaaac cacctgcagg
     4801 aagactactt caaagcctgt tttgtggaaa tactgaacag agcaaaacaa aaatatcatt
     4861 ttaagctcat tgccttctgt gttatggata atcatatcca tttagttatt cacacccttg
     4921 aggatggtgc gcccatttcc cgtattatgc agtatattaa ggcgcggttt gctgaactgt
     4981 ataataaaat aaccggcaga accggtcctt tctggaatga gcggtataag gatagcatca
     5041 tacagtttgc aaaggatggg tttcactatt tgttgtggtt attatggtat ctggcatata
     5101 atcccatacg aaagaggtta tgctctgacc ctataaaata ccactacagc agcattaaag
     5161 catatttaca ggaagaagct gatgtaggag tacccataga ccatcatgag tactttgtta
     5221 atcttgggga aacgtttaca gagagggtgg caagattcat gcagtatgag gaatattaca
     5281 ggaagaagta ttccatgata cttgagtggg tgtaaaaggg gtcagagctg ttattaagtt
     5341 agtaattacc acgctattac tgacttatac ttatttgttt taacaacaat atccccagtt
     5401 gctggtgtat attgcactaa gttacaatta ataagctgct tatactgttt ggggatacgt
     5461 gctcgtatat aataattgtg ccctgctccg ccaggagtgt atatttcaat actgttaccc
     5521 ttaactttaa aattaacagt agaagaatta tatacaacaa actcatagaa agcatctttc
     5581 acagtcatcc atcgtatcca ggggtagttt tgctttaatg aaccaaattc tttttcaaag
     5641 ttgcttttca gccaaagcca cccttcataa gattttgaac gcatttcgtc aaagacatca
     5701 tcaggatgga taaaatggct gattattcca aaattttgga acgcatcgta caaagcaaat
     5761 ttatcaaaat cctgaaaata atatcctgat gtaatacgtg gtatctggta aaacctgtta
     5821 tcaggtgacc agtcaaattc ttgttctact ccgccacttt ttactacata tagcgtcgac
     5881 acaactcgta tactggggaa aacctctccc agcgcctgca atcctgttgc atcaattata
     5941 ttatgcggtg caacatagct aaaagggaga gcactttcgc catacagtgc tatccattca
     6001 ttttttgcta cagtgagagc ctgaaccatt gtttttttat ccggccacgg tactgagtca
     6061 tactcttttg cctttgtaag agttaatgac atatggttgt acccatgcag gccaagttca
     6121 aacccatttt caagagctgc tattcccatt ttagtactaa gtccattctg cgataaatag
     6181 aaatctcgta ctgcaaagcc tgtttcatct gcttttgtat cacggttata attaaaaatt
     6241 aaaaaactgg aataagcaaa tccaaactgc ttttgccatg ccagtatatc tttaaaccat
     6301 acatcagaaa taaaaagggt gtctgtttct tcaggtagtt tctttaattt ttctatgatt
     6361 tcttccagct tcttgcggtt gaatttatcc ttttcgtttt gtaacaagtc actgtaatat
     6421 ttaattctgt atggcttata tgcaacattc caccatggtg cagggaaatc gtcgatcata
     6481 attaaaccaa catttatcat ccctgttgca aacacgtctg caattttata aatgctctga
     6541 acaatcatcc cgcgggtcaa cttagattta tccagcaaat tagtattcca gtacaatatc
     6601 ttacctttct taattgttcg ttcccataca acaggatatt tatctgcatc aaataatgta
     6661 ttaaccgtac caataacttt gacatcactt tccagaactg ccctgcgaac attacagtct
     6721 atatcatcac cgggtacatt tactccattt aaaaacaatg agggttgtaa cgtaaaaccc
     6781 tgtttaaaat attcttcttt ttttatatat tctttaattc ctataaacgt attcaattca
     6841 ttatctggtt caacataatt taaaaatata cattttccac cattttcaac ataacctttc
     6901 aataacttta atggaacagc atctgcagtt ttagttgcca ccagtagcgc ttcatattca
     6961 gttaaatcat tagccaccaa tccttttatt tcttcttttg tatacacatc acagcgaatc
     7021 tttgcatatt taaaggcata gagtatatta ttgtttacta catttgatct gtctatgtca
     7081 ccaattatca cacccacatt aagatagcca tcatcataaa agagtgcttc atgtataaac
     7141 cgtgatgtat tgagcattac tttgctccca cgaaccacgt agttttctaa tgcaaatgct
     7201 cccgtctttg atataacaat aatacttgat ttgccatccc ctgcttgagg cggtgcatcg
     7261 gttcgttgta gctgtaaata tgtctttaca tcttcacgca atgggataat ctgctgatag
     7321 tgcaatgatt ttgttacatc ctgcttttca tccttttcta ccattgcact gtctttatat
     7381 atgatcttta tattgtttgg attgttggtc cagaatccct ggaagtaaat ccctatcttt
     7441 gtaaaaagag ggcgtatata gggaagtaaa tcccattttg tttcgttgcc atccttatag
     7501 ccatatgcac cgtaggaatt gacaatgata atttttatcc ctttattggc tgcatcaatc
     7561 atgaactttg catagtttat acctatgtcc ttacttgcca caacaccgcc attataccag
     7621 gtaactatcg cccgtatatc atcaagatta cttaacgatg aaaagccctt atcaaaatca
     7681 taatatttta cctgcaaacc atttgcggta atatcctttt caaaaaacca ttttaacggg
     7741 ttactatcat cggtataccc ttcggatgat ttatagatac acaataccga aagccctttt
     7801 gcttttgcaa aagaactcac tgtgctaaat ataacaatac agaaagatac tgttattaaa
     7861 gcgatagttt taataaagct caatttcata acgtatatcc cattttgaag gtttatcttt
     7921 aaggtactgt acttctgcac ctgcaccaac agtttttatt tctgcagcaa gattttttaa
     7981 tctttcagct attactgcag cctgtttttg cgataaagcc atattggatt gcttgcttcc
     8041 tacagcatca gtataggcaa caatattaat ccttgtaaag ccacccatct ttgccagttt
     8101 acctgcaagc tggtcaagta tcagcacccc atgggttgta agctcatcac ttcgtccttt
     8161 aaatattgta tcctgattga acatatatac aatacgtttg cccttctcct gctttatcag
     8221 aaatcccacc ataaacggta ctttctttga tactgcggta ttgcctgcat catctgttat
     8281 ctccatctgg gcttctaatt gcatattata cactgcaggt tcacctttat catcggtccc
     8341 atcccaggaa attactttag gcggcatgcc tgcaccatta attactttaa caacagcacc
     8401 atcatcgcgc ataaatctaa tctgccactg cgcaacatct ccatcaactg atggagtaat
     8461 acgcaatggt gtttgcagcg gtgtaatttc atttggctca acttttatgt caactgctac
     8521 tttagtaacg acagtaacag tagcagctgg agacttaacg acaatcccat catcatattt
     8581 tatagtaagt gcagctacat attctccatc aggtactgtt ttgagattat catctaatcc
     8641 gtcccataat acatcatacg ggataaagtc atttcctcta attgttttta caattctatt
     8701 tttattgctt atagaaacaa accagctggc tatgtttttc ttgtccgttg cctgaatagt
     8761 aaaacttact gtatccttaa ttttatcatc atttggggaa aatttgggat atgaaggggt
     8821 cagagcaata gcacgttttg ccgcagatag tacaatctcc tgctttttag agttgaatcg
     8881 ctcacctgca gaattttgta tggtaagctg aacatagtat ttgccttccg cagccagcac
     8941 attgtttctt gccgtaccat cccatctgaa tgttcccggc accgcaccag caaggctata
     9001 ttcctgaatc ttctgctctt tttcatcata aatttcaatc caccactgct ggataccttt
     9061 ttcatcaaat gcagttaaac tcaacgttgc aaagtcatcc ttgccatcgc catcaggtgt
     9121 aataagcgtt ggattggctg acacaaatag tactggttta ccaccggccc cttttgggct
     9181 atatatgctg aagaatttac ctgttgcacc aaacttcagg tcatgtgaga tatatgtttt
     9241 atcagcagtg gatttgggca gagtaacatt tgaatacgta tactctgcaa acagattaag
     9301 tttatcccag ctctgtgaaa gtgagattcc tggcatcatt atgcgctgat caagtgaatc
     9361 atattgatac tgcaaaaata cagaatacaa caaccgcccg ccaaaaagat tttcaacctg
     9421 ctgcgttatt ttaaccattg ggccataata agaaacaccc tgcggagccc agtatggaac
     9481 aagtagtata tctccggaag aatactggtt aaattttgta tcctcgtacg aataaatacc
     9541 tgcaatatca aagaacatat tccattgcgg tatacgatat actcgtactg taagctggtt
     9601 aaagctagac aatcgagtat tggtatccga tagcatgcgg tattgaagcg cattatacca
     9661 gaaaagccgt ttactcaatg ggaaatcata ttcagaaaaa tttatatcaa gaataccctc
     9721 aaacatatca tacgtaatgg catctctaaa cttcatcttt aacgactgtg ttagatcatt
     9781 ttcatcatca tgactatacg ctacttttac ggtaactcca gtacctggcg gcatccacca
     9841 gaacccagcc ccataggaca atgacatata acttagtgat gtatcataca aaggattgcc
     9901 gtaatctacc gtacccgaat acatggtacc gcccagatgt gcaaaaagcc gcatatacaa
     9961 aaacctgaag ggaactgcaa tttcagcaaa agctttgtgc attgtcacat tccctttaga
    10021 tggataaaag agctgcccgg ttgtagcaat gcctccagaa tcagtttgtt tattcaattc
    10081 atagcccgcc tgtaatgcaa aatactggct gggctcatat agaccactta agccaatacc
    10141 atagcgtgtg cgtatattgt cactaaaata tatactattg ccagtaatag aaggagcatg
    10201 ttgtttttgt aattcataat gagccttacg tgcatattca aaatccggct gtgcattaat
    10261 gcatatttca tagtttccct tagcatcctt ccagtatccc aaaaattcat tgatattgcc
    10321 catagcaaaa tttgcttcca cgttcaacgg ctgcactttt aaaatatttg aataatattc
    10381 tcgtgaggca ttataattgc cgttttgcaa atagaagttg gctatttctt tataaatacg
    10441 cgcattgtct atctccaggt ctaagaactg cttttcatac acattgcgca ttgtggcaag
    10501 gccaaatgaa agcttttggt taacatcatc atcaatagat tccagtaatg agtctgtatt
    10561 tgccagtaaa acttcaatat cctgaagtgg taactactca gaaagtagag gcacactcag
    10621 gggaaaatat agattgtttg atta
//
LOCUS       DAOOJH010000083        10227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_5859_length_10227_cov_10.282540, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000083 DAOOJH010000000
VERSION     DAOOJH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5859_length_10227_cov_10.282540"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1356)
                     /locus_tag="PLJ75_09855"
     CDS             complement(79..1356)
                     /locus_tag="PLJ75_09855"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPD05555.1"
                     /translation="MTLLIKNGRVIDPLSKLDRCCDILIDEGTIVTIDTNIKQQRNIQ
                     IVDAQDCIVLPGLVDMHVHFREPGREDIETIAGGSTVAAKSGYTSVCTMPNTNPVIDS
                     QALVRYIKKQAESGFINVHPIAAITKGSLGEELTEMGELIEGGAIAFSDDGKPVLNST
                     TMRRALEYSRMFNVPIITHAEDTTLSGGGIMNEGNNSIFLGLKGIPKEAEEIMIARDI
                     LLARLTKGRLHIAHVSSKGSLDIIAMAKQHSLPVTCETAPHYFSLTDDAIADHLAMAK
                     MNPPLRTEEDRKAIIEGLRTGLIDVIATDHAPHLSNEKMQEIVFAPFGIIGLETAVPL
                     IITILVKENGFSYLQAFEKVTINPCTILGIDRGYIKEGAVADITIVNPEKKVIVDNNF
                     IISRCKNTPFLDREFFGLVTHTICNGKIVYPFR"
     assembly_gap    1418..1517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1518..2458)
                     /locus_tag="PLJ75_09860"
     CDS             complement(<1518..2458)
                     /locus_tag="PLJ75_09860"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPD05556.1"
                     /translation="MNGLQRKDLLDIQSLSTEEILLICNSAKYFKALFTRSIKTVPIL
                     RGKTVCLLFYEPSTRTRISFELAAKRLSADLINIAVQTSSVVKGESLIDTVHTLEAYK
                     ADFIVMRHAASLAPHFLSRNIKSSVINAGDGNHAHPTQALLDAYSLIEKRGDIKGLHI
                     GIVGDILHSRVARSNMEIFKRLGAKVSLIGPPTLVPDDFTMYAVDIYYDFDTILPQLD
                     VVYMLRIQRERQQGSFFPSIHEYHKRFALTNERLKRCKNNVIVMHPGPINRGIEIDDE
                     VADSPCSIINEQVTNGVAVRMAIFYLLAGGAPLDEKEV"
     gene            2675..3328
                     /locus_tag="PLJ75_09865"
     CDS             2675..3328
                     /locus_tag="PLJ75_09865"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPD05557.1"
                     /translation="MSEYIVFYDLDHTLIAASSGQLIVKDLYQRHMLSLLTLGKGMVL
                     STLYRMGFISTESLIQKWVAKLAGMQCSFLERYSETFFDQTVKKYIYPQALESLYIHK
                     AQGAYLVLLSASLDFICKPVMKYLHFDAMLCTQLEVNNDHYTGSIKGSYCYGKEKLNR
                     ALQLCHNHNFNIHDAYYYADSLADIPVLEAVGHPHCVNPDQKLLRIAQSQGWTIHRW"
     gene            complement(3330..4976)
                     /locus_tag="PLJ75_09870"
     CDS             complement(3330..4976)
                     /locus_tag="PLJ75_09870"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004397381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPD05558.1"
                     /translation="MKIAIAQIDPVIADISGNYQIISKYVHQAIQHGAELIIFPEMAT
                     IGYPPMDLLEHKKLIDDNLKSIETIATLSKDCGIICGYVDYDHDNPPMLLNAAACMYN
                     GNIQSKHYKTLLPTYDVFDELRYFSPASIQEPMKFKGVNIGLTICEDIWNDMSISEDS
                     FMEYRRYHVDPVENLIKKGAEFIVNISASPYVKGKNSIKWNKIQHVAKKYRVPIVYVN
                     QVGCNDSLIFDGSSFVIDTQGNFIMKAKAFEEDCVIFDTAVAYQPIVCEEDFLSDCTN
                     ALVLGIKDYVYKSGFNKVVLGLSGGIDSALTVCLATRALGADNVIGVTMPSVFSSKGS
                     VDDSIALAKNLGITIHTIPINDCYDTYLDILKPYFNALPFDSTEENIQARIRGNILMG
                     FSNKYGMLVLTTGNKSELSMGYCTLYGDMAGGLAVLSDIPKTMVYALSYYINRDKEII
                     PQSILDKAPSAELRENQKDQDTLPPYEILDQIIERYVELKMSAEDIINDGFDSAIVYN
                     VLRTIDKNEYKRKQAPLGLKVTGKAFGVGRRIPIVQRFKH"
     gene            complement(5020..6303)
                     /locus_tag="PLJ75_09875"
     CDS             complement(5020..6303)
                     /locus_tag="PLJ75_09875"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD05559.1"
                     /translation="MKKMSVIIIVLIACITNTFADVDCSKILNEQIYDEQSYANLSDE
                     CIENFKYIIVKGKDTEQKLKALKILRKSKVGKNCDFFKDILLSVNSMTVQQMIIEIFS
                     DIHDKQVLPIILPYLESPFYAVREAVIMALKKNGDDRMYPHIIKLAKSDDVINRIYAL
                     EALYHLYDNRFYEIVVQLLKDQNKSVRYFALECVKHNEIIATLPYIRAMAINDDNDEI
                     KVEAILVLASLHDKGSYYIFVRSLGSYSDGVREAAVDALIQFPSRDSALYLSRQLLAE
                     DETYIKRKIVKALITFRTAGDIRGLQKILENDENPSLRVYAAYALGVIKDDRTITMLI
                     RATGDDDYRVRAEAANALGNFKGKIVVISLLEKYEKESSTYVKTAILYSLKKICDKGS
                     LQKLFTLYCNENDVIHKMILQQTIHHIIARSFNQY"
     gene            complement(6415..7023)
                     /locus_tag="PLJ75_09880"
     CDS             complement(6415..7023)
                     /locus_tag="PLJ75_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05560.1"
                     /translation="MPAKGQKRRQQIVDTAKKMFIEQGFQSTHIGQVCEALNIARGTV
                     YQYFGNKREILYAILESIEESIDDILDPDDLRDLLKDNPSQKDIIKFNTERITECVKA
                     IIEEPIVIKLVFKDIVGIDEEVIERVDQFLKHLINIVSRDVEEIKKKGLYKKTLHSDI
                     TAIILIGSIMLLVYQFDKEKKDVTDKEVINALVSHYFTGVLK"
     gene            complement(7395..8087)
                     /locus_tag="PLJ75_09885"
     CDS             complement(7395..8087)
                     /locus_tag="PLJ75_09885"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05561.1"
                     /translation="MEELALHKQIQQLLQEAHLLLSEKKNIRGAIATLEKLEKMDPEN
                     PLVLYNLAIAHAHCEDYARTAEYAVRCIALPMTFIDVKQVKKVYIYALIQSEDYEKAF
                     HEIETYDDIDEVLLQFEAFALEKMGDYNHALMIYERIYKKNSNNINVCNAIAYILANL
                     PEGNLDRALELAKKAYQREPHNAAYCDTIGYVYFKRGQLNMAKKYLKKAFAIKPDHRE
                     IRKHIAELLNIP"
     gene            complement(8075..8836)
                     /gene="surE"
                     /locus_tag="PLJ75_09890"
     CDS             complement(8075..8836)
                     /gene="surE"
                     /locus_tag="PLJ75_09890"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPD05562.1"
                     /translation="MNILITNDDGINAQGLQVLKGVLSDHYSVFSIAPGTERSGCSNA
                     IHMRTEITVEQIDEYSFAVDGFTADCVNIGLHSGLIPPVDLVISGINHGPNMGDDIYF
                     SGTVAGARTAYIFGVSGIAVSIDSYQESDYFKDASYFILELLKDYPIHVKKYPWFLNI
                     NYPDLPCHAIKGIQYTQLGKRYYRDAYRIVKQSGKFFTLELDGTIENEEKAGSDVTEL
                     RNGYIVITPLSIDCTDYNYMNRLQKRATTNHIWKS"
     gene            complement(9183..>10227)
                     /locus_tag="PLJ75_09895"
     CDS             complement(9183..>10227)
                     /locus_tag="PLJ75_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPD05563.1"
                     /translation="CGLYILERLVYTLHKAGIQHIVLHLDSDEKAFFNKTILHYITKK
                     NIIVEECPNNKKPEKGYLVLPSNLFIQPHYFDNFKTYFKESNKMYEPLLREDQFLINS
                     INDVKKAESLIVKYIIENTGGYIAKHINKRISIPISLQLAKTRIHPNILTVINMMVGI
                     VSAYCITQATYWSMVIGGLLFQLASIFDGVDGEVAKFTFTVSKIGGWLDTIGDNGTLL
                     LFLIAVSWLYFKNTSIGVACLVVSLCFIGLFWFIGMMVWYLRKYSESGSLVAYDKEFL
                     QKLPRDDVFVKVALSMKYITKKEFFALFFFCFSFTGKIFLLIPIITFVVCAGACVLTI
                     INIKYLKAVNTIR"
BASE COUNT         3133 a         2054 c         1564 g         3376 t
ORIGIN      
        1 atgagctggt acagtttgat ggagtgcatc caaccgaaga gggatactat atcatgtcat
       61 tagcattaca ggagctattt acctgaatgg gtaaacaatc tttccattgc agattgtatg
      121 ggtaaccaat ccaaaaaatt ctctgtctaa aaatggggta tttttacatc gtgaaatgat
      181 aaaattgtta tcaacaataa ccttcttttc agggtttact attgttatgt ctgccacagc
      241 accctctttg atatagcctc tatcgatacc taaaattgtg catggattga tggttacctt
      301 ttcaaatgcc tgtaaatagc tgaatccatt ttcttttacc agaatggtaa taatcaatgg
      361 tacagcagtt tccagtccta tgataccaaa tggtgcaaat actatctcct gcatcttttc
      421 atttgataag tggggtgcat gatcagttgc aatgacatca ataagccctg ttcgcaatcc
      481 ttcaatgatt gcctttctgt cctcttcagt ccgtaaggga gggttcatct ttgccattgc
      541 cagatgatct gcgatagcat catcagttaa cgaaaaataa tgtggtgctg tttcacaggt
      601 gacaggcagg gaatgttgtt tagccattgc aataatatcc agtgaacctt ttgaggatac
      661 atgggcaatg tgcaatcttc cttttgtaag ccgtgccagt aatatatccc ttgctatcat
      721 tatttcttca gcttctttag ggattccttt caatccaagg aagattgaat tattaccttc
      781 attcattatt ccgccaccgg acaatgttgt atcctcagca tgggtaatta ttggaacgtt
      841 gaacatcctt gaatattcta atgcccggcg catcgttgtg gaatttagaa cgggtttgcc
      901 atcatcacta aacgcaattg ctccaccctc aatgagttcc cccatttccg ttagttcttc
      961 tcccaatgac cctttggtga ttgcagcaat gggatgcaca ttaataaatc cggactcagc
     1021 ctgttttttt atatatcgca caagtgcctg actatcaatg accggatttg tattgggcat
     1081 ggtgcataca ctggtatacc ctgattttgc agccaccgtt gatccgcctg caatcgtttc
     1141 aatatcttcg cgccctggct ctctgaaatg gacatgcatg tcaacaagac caggaagaac
     1201 aatacaatcc tgggcatcaa ctatctgtat atttctttgt tgttttatat tggtatctat
     1261 ggtgacaata gtcccttcat caatcaggat atcacagcat ctgtcaagtt tagataaggg
     1321 atcaatgact ctaccatttt taataagcaa tgtcattatt ccacctcttt ttcatcaagt
     1381 ggcgctccgc cagctaataa ataaaatatt gccatccnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnacc tctttttcat caagtggcgc tccgccagct aataaataaa
     1561 atattgccat ccgtaccgct actccattgg taacctgttc attgattata gagcagggac
     1621 tatcggcaac ctcatcatca atctctatcc cccggttgat gggacccgga tgcatcacaa
     1681 taacattgtt tttacatcgc tttaaccttt cattggttaa agcaaaacgt ttatggtatt
     1741 catgaataga aggaaagaat gaaccctgct ggcgttcccg ctgtattcgt agcatataaa
     1801 caacatcaag ctgtggtaag atagtatcaa agtcgtaata gatatccacg gcatacatgg
     1861 taaaatcatc cggtaccaat gtaggcgggc ctattaaact tacctttgct cccagccgct
     1921 taaatatctc catatttgaa cgcgctaccc gtgaatggag tatatcgcca acaatcccta
     1981 tatgaagccc tttaatatct cctctttttt caatgagtga ataggcatcc agcaatgcct
     2041 gtgttggatg agcgtgattt ccgtctcctg cattgattac cgatgatttt atatttcgag
     2101 ataaaaaatg cggcgctaat gacgccgcat gtctcattac tatgaaatct gctttgtacg
     2161 cttctaaagt atgtaccgta tctattaaag attcaccttt taccacactg gatgtctgta
     2221 cagcaatatt aataaggtcg gctgaaagac gttttgcagc caactcaaaa cttattcttg
     2281 tccgtgtcga tggttcataa aacaataaac atactgtttt acctctcaat atgggaacag
     2341 ttttaataga acgtgtaaaa agggctttga aatatttagc cgagttacag atcagcagta
     2401 tctcttcagt tgataacgat tgaatatcta ataaatcttt tctttgtaac ccattcatag
     2461 agtactataa accttatata aactgatatg aaaaaagctg caacttgcac agtattaatg
     2521 cattcataca tcctcttttt atatcaattt tatgaaatta ctattaattg caatcatatt
     2581 ttcaataatt tttgtaaaat atggtgtcat tgtactattc ttataacatc catgtatatt
     2641 catcctgtaa atttttttat tgtatggtgt ttgtatgagt gaatacatag tcttttacga
     2701 ccttgatcat acactgattg ctgcaagttc agggcaatta attgtaaaag acctttacca
     2761 gcgtcacatg ctatcattgc ttacccttgg caagggtatg gtactttcaa cactttaccg
     2821 catgggtttt atcagcacag aatcattaat acagaaatgg gttgcaaagc tggctggcat
     2881 gcaatgcagt tttcttgaac gctattcaga aacttttttt gatcaaacag taaaaaaata
     2941 tatctacccg caggcactgg aatcattata catacacaaa gcacagggag catatcttgt
     3001 attgctatct gcatccttag attttatctg taaacctgtc atgaaatatt tacattttga
     3061 tgcaatgctt tgcactcaat tagaagtaaa caatgatcat tacaccggtt ctattaaagg
     3121 atcgtattgc tatgggaaag aaaaattaaa cagagcattg caactatgtc ataaccataa
     3181 tttcaacata catgatgcct attactatgc tgactcattg gcagatattc cggtactgga
     3241 agctgttggg cacccccact gtgttaatcc cgatcaaaaa ttattgcgca ttgctcaatc
     3301 acagggctgg acaatccata gatggtgatt taatgcttaa accgctgaac aattgggatg
     3361 cgtctcccaa caccaaaagc tttacctgtt acctttaacc ccaggggtgc ttgttttcgc
     3421 ttgtattcat ttttatcgat agttcttaga acattataca ctattgctga atcaaagcca
     3481 tcgttaataa tatcttcagc actcatcttt aattcaacat atcgttcaat tatttgatca
     3541 aggatttcat aaggaggtaa tgtatcctga tctttttgat tttctcgcaa ttcagcagaa
     3601 ggagctttat ccagtattga ttgaggtatt atttctttat ccctgtttat ataataggag
     3661 agtgcatata ccatggtttt gggaatatca gataatacag caagtcctcc tgccatatcg
     3721 ccatataatg tacagtatcc cattgacagc tctgatttgt tccccgttgt caatactaac
     3781 ataccatatt tattgctgaa gcccataagg atatttcccc ggatgcgggc ttgaatattt
     3841 tcttcggtgc tatcaaatgg taatgcatta aaatatggtt ttagtatatc aagataggta
     3901 tcatagcaat cattgatagg aatggtatgt atggtaatac caagattttt tgccagtgct
     3961 attgaatcat ccacacttcc ctttgacgaa aaaacagaag gcatggtaac ccctataaca
     4021 ttgtcagctc ctaaggctct cgtggcaaga cagacagtta acgctgaatc aataccacca
     4081 cttaaaccca acaccacctt gttaaaaccc gacttatata cataatcttt tatacccagc
     4141 accagagcat tggtacaatc agataaaaaa tcttcctcac agacaatggg ttgatatgca
     4201 actgctgtat caaaaattac gcaatcctct tcaaaagctt ttgccttcat tataaaattg
     4261 ccctgtgtat cgatgacaaa actactccca tcaaaaataa ggctatcatt acaaccaacc
     4321 tgattgacat aaactatcgg cacacggtat ttttttgcaa catgctgtat cttgttccat
     4381 ttgatggaat tcttcccctt tacataaggt gatgcagaaa tgtttactat aaactctgcg
     4441 ccttttttta tcaaattttc aacagggtca acatgataac ggcgatattc cataaaacta
     4501 tcttcactta tactcatatc attccagata tcttcacaga ttgttaaacc tatgttcacc
     4561 cctttgaatt tcattggttc ctgaatggat gccggcgaaa aataacgtaa ttcatcaaac
     4621 acatcatacg taggcaaaag tgtcttgtaa tgctttgatt ggatgttgcc gttgtacata
     4681 catgcagcag cattcaataa catgggggga ttatcatgat cataatccac gtacccacaa
     4741 ataataccac aatccttact caatgttgcg atagtttcta tggatttaag gttgtcatca
     4801 ataagttttt tatgctccag taaatccatc gggggatagc caatagtagc catttcagga
     4861 aaaataatca attcagcacc atgttgtata gcctgatgaa catatttgct gattatttga
     4921 taattacccg aaatatctgc aatgaccgga tcaatttgcg ctatagctat cttcataaag
     4981 ctacctttta cttccattat agtattgtaa aattacatgc taatattgat taaaacttcg
     5041 tgctattata tgatgtatgg tttgctgtaa aatcatttta tgaatcacat cattttcatt
     5101 acagtataga gtgaataatt tctgtaacga acctttatca caaatttttt ttaatgaata
     5161 gagtattgct gttttaacat acgtactgct ttccttctca tacttctcca gcaaagaaat
     5221 gacaacaatt tttcctttga aatttcccag tgcgttagca gcctcagcac gtaccctgta
     5281 atcgtcatca cccgttgctc ttatgagcat tgtgattgta cgatcgtctt taatcacacc
     5341 aagagcgtag gctgcataca cacgtaacga tggattttca tcgttctcca gaattttttg
     5401 caaaccgcga atatctccag cagtcctgaa cgttataaga gccttaacta tttttcgctt
     5461 aatataggtt tcatcttcag ctaataactg tcgtgataaa taaagagcac tatctcgtga
     5521 aggaaattgt attaatgcgt caactgctgc ttcccttacg ccatccgaat agctacccag
     5581 tgatcgaaca aaaatataat aggaaccctt atcatgtaat gaggctaaaa ccaggatagc
     5641 ttcaaccttt atctcatcat tatcatcatt aatagccata gcacgaatat agggtagggt
     5701 tgcaatgatt tcattgtgtt taacgcattc aagtgcaaaa tatcgcactg atttattctg
     5761 atctttcaat aattgcacaa caatttcata aaatctattg tcatacaggt ggtataatgc
     5821 ttccagcgca taaattctat tgataacatc atcgcttttt gctaatttaa taatatgagg
     5881 atacatcctg tcatcaccat tcttcttcaa tgccattatg actgcttcgc gtactgcata
     5941 aaaaggtgat tctaaatagg gtagtatgat gggcaagacc tgtttatcat gaatatctga
     6001 aaaaatttca attatcattt gctgaacagt catagaatta actgataata atatatcctt
     6061 aaaaaaatca cagtttttac ccactttact ttttcgtagt atttttaacg cttttagctt
     6121 ttgttctgta tctttacctt tcacgataat atatttaaaa ttttcaatac attcatctga
     6181 aaggtttgca tacgattgtt catcatatat ctgttcattc aaaatctttg agcaatcaac
     6241 atcggcaaaa gtattggtaa tacaagcaat aagaactata ataatcacac tcattttttt
     6301 cataaaacta tcaccataca aatatttgag tcacaatatt gatatatatg atttatatca
     6361 acaaaaaacc cggctcatat aagccgggat ataataccac tattgtcgca acaattattt
     6421 taatacaccg gtgaaataat gagaaacaag tgcattgata acttccttat ccgtaacatc
     6481 ctttttctct ttatcaaact gatataccag cagcataatt gacccaatga gaataatagc
     6541 tgtaatatca gaatgtaatg tctttttata aagacctttc ttttttatct cttcaacatc
     6601 tcgtgatact atattgatta aatgttttaa aaactggtca acgcgctcaa taacttcttc
     6661 atcaatacca acaatatctt taaaaactaa ttttattact atgggttctt ctataatagc
     6721 tttcacacat tctgtaattc tctcagtatt aaattttata atatcttttt gagatggatt
     6781 atcttttaaa agatcgcgca aatcatcagg atcaagaatg tcgtcaatac tttcttctat
     6841 tgattctaaa atagcataga gaatttctct tttattacca aaatattgat aaacagtacc
     6901 ccgtgcaata ttcaatgctt cacaaacctg tccaatatgt gtgctctgaa atccctgctc
     6961 tataaacatt ttttttgcag tgtcaactat ttgctgtcgt cgtttttgtc cttttgctgg
     7021 cattataata cctctttcat tcaatagtaa tatattattc tatttaagtt cccctttcac
     7081 acgcaatagt tcttctttta taggctgtaa tgcttcctga agatctttaa tcatcttttc
     7141 tgctttttcc tgcaattctt tactcataga gtaacaatcc tctttcgcca taaatattca
     7201 atgctataat acctatcgtt aacatttata tgaaattata taaaaaaaga ttattgaatt
     7261 taaactttat atctaacctt ctttttcacg tcaacaactt tttataaatt taatattgtt
     7321 ataattcaac actttggcat aataagaaca tattataact atctccagca tatgattttc
     7381 acaacattat tatgctatgg tatatttaat agttcggcaa tatgtttgcg tatttccctg
     7441 tggtcaggct ttattgcaaa agcttttttt aaatatttct tagccatatt cagttgcccc
     7501 cttttaaaat atacataccc aattgtatca caatatgccg cattatgggg ttcacgttga
     7561 taggcttttt ttgctaactc caatgccctg tccagattgc cttctggcag gttagccagg
     7621 atgtatgcga tagcattaca tacatttata ttattagaat tttttttata aattctttca
     7681 tagatcatca atgcatggtt ataatccccc atcttttcta aagcaaatgc ctcaaactgc
     7741 aatagcactt catctatgtc atcatacgtt tctatctcat ggaaagcttt ttcataatct
     7801 tctgattgaa ttaaagcata aatatatacc ttcttaacct gcttcacatc aataaaagtc
     7861 ataggcaatg caatacaacg gactgcatac tctgctgttc tggcataatc ctcacaatga
     7921 gcatgggcta tagccagatt atacaaaact aatggatttt cagggtccat tttttcaagc
     7981 ttttccagag tggcaatggc acctctaata tttttctttt cgctaagcaa gagatgtgct
     8041 tcttgtaata gctgttgtat ctgcttgtgt aatgctaact cttccatata tgatttgttg
     8101 tcgctcgttt ttgcaatcga ttcatataat tataatcggt acaatcaatg gataatggtg
     8161 taataacaat atacccattg cgcaactcag ttacatcgct cccggccttt tcttcattct
     8221 caatggtccc atccaattcc agggtaaaaa actttcccga ttgttttaca atacggtatg
     8281 catccctgta ataccgtttt cctaactggg tatactgaat ccctttaatt gcatgacacg
     8341 gcaaatcagg ataattgata tttaaaaacc atggatattt ttttacatga attggataat
     8401 ccttcaataa ttccagtata aaatatgacg catctttaaa ataatctgat tcctggtagc
     8461 tgtcaataga cacagcaata ccgcttacac caaaaatata tgctgttcgt gctccagcaa
     8521 ccgtccctga aaaatatata tcatcaccca tattggggcc atggttaata ccggatatca
     8581 ccagatcaac cggtgggatc agcccgctgt gtaaaccaat attgacacaa tccgcagtaa
     8641 aaccatcaac agcaaatgaa tattcatcaa tctgttctac tgtaatctct gtgcgcatgt
     8701 gtatggcatt ggaacagcca ctacgctccg taccaggcgc aatggaaaaa acagaataat
     8761 gatctgataa aacccccttc aatacctgta atccctgcgc attaatacca tcatcattgg
     8821 taattaaaat attcatacgt tactcgtgat agttactgta ctatagacat tttttataaa
     8881 aaataaatag gtgctataat tgtattatag aatactcctg tgatggtgcg tctatgccaa
     8941 tctacgaatt taaatgtaac aattgcaacc atatctacag cgaactcagg aagatgggcg
     9001 atagtaaccc ttcaccatgt cctcagtgcc attcacataa tactgaaaaa attatatcac
     9061 tgataagtgc ctctgtccat aaacaatctt gttcagcatg ctcagcaaaa aactgttccc
     9121 attgtaaata acattattcc ttcccatgca agcactctat taccagatac tatcgccaag
     9181 ggctaccgta ttgtattaac tgcttttaaa tactttatat tgatgattgt cagcacacag
     9241 gctccggcgc atacaacaaa agttataata ggtattaata aaaatatctt cccggtaaag
     9301 ctgaaacaaa aaaagaatag cgcaaaaaac tctttttttg taatgtactt catggaaagg
     9361 gcaactttta caaacacatc atcgcgtggc aatttctgca aaaattcttt atcatacgct
     9421 accagagaac ctgattcact atatttcctt agataccata ccatcatgcc aataaaccag
     9481 aacaaaccaa taaaacaaag gcttacaaca aggcaggcaa cccctataga ggtattttta
     9541 aaatataacc atgacacagc aatcagaaac aaaaggagcg ttccgttatc accaattgtg
     9601 tcaagccagc cgccaatctt tgataccgtg aacgtaaact tcgcaacctc accatcaaca
     9661 ccatcaaaaa tggatgccag ttgaaataat aatcccccta ttaccatgct ccagtaggta
     9721 gcctgagtta tgcaataagc acttacaata ccaaccatca tattgatgac tgtcaatata
     9781 ttgggatgaa ttcttgtttt tgctaattgc aggctaatgg gaatggagat acgtttatta
     9841 atatgttttg ctatatatcc accagtgttt tcaataatat actttacaat caatgattct
     9901 gcttttttta catcattgat tgagttaatt aaaaattgat cctctcgcaa tagaggttca
     9961 tacatcttgt tgctttcttt aaagtatgtt ttaaaattat caaaataatg aggttgaata
    10021 aatagatttg atggcaatac cagatagcct ttttctggtt ttttattgtt tgggcactct
    10081 tccacaataa tatttttttt tgttatatag tgcaatattg ttttattaaa aaatgccttt
    10141 tcgtcactat caagatgaag aactatatgc tgtatccctg ctttatgaag cgtatacact
    10201 aatcgctcaa gaatgtataa tccacag
//
LOCUS       DAOOJH010000084         9968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6035_length_9968_cov_11.357813, whole genome shotgun sequence.
ACCESSION   DAOOJH010000084 DAOOJH010000000
VERSION     DAOOJH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9968
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_9968_cov_11.357813"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PLJ75_09900"
     CDS             complement(<1..400)
                     /locus_tag="PLJ75_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HPD05564.1"
                     /translation="MNTTLDVKSLENWLWEAACKIRGPVDAPKYKDYILPLIFFKRLS
                     DVFEDELDRLSQDYGSKETVEKLLAQDHKLVRFYLPKDARWEAVANHTTNVGEHLTNA
                     VRSIAHENAKLQGVVDTVDFNATTSGQRILG"
     gene            643..996
                     /locus_tag="PLJ75_09905"
     CDS             643..996
                     /locus_tag="PLJ75_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05565.1"
                     /translation="MKKNELGMKTGKGIYAWQGGKAIIDTSHKTDIIKPVDPLAVQLN
                     EAIRVLKEKVARSAEDIDRGQEAGMNQPGPFKTAAGIDHNLIARRLQWLSAAYNLSYI
                     NPEPEILDGSFKSMI"
     gene            1010..1189
                     /locus_tag="PLJ75_09910"
     CDS             1010..1189
                     /locus_tag="PLJ75_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05566.1"
                     /translation="MEYIETQVKDAVACCYINRNEVKNALNRTIIDELGAKIGSLWGQ
                     SPDYDFYGTYMGKEL"
     gene            1161..1388
                     /locus_tag="PLJ75_09915"
     CDS             1161..1388
                     /locus_tag="PLJ75_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05567.1"
                     /translation="MERIWEKNYDFWYNPIHFQRMTIQDMLHCSAAVTPDKVATDFYG
                     TKITYYGLRKMSITFTQSLVNIGIKNVIESR"
     gene            complement(1394..2029)
                     /locus_tag="PLJ75_09920"
     CDS             complement(1394..2029)
                     /locus_tag="PLJ75_09920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05568.1"
                     /translation="MHNVILLLALVSLMGTPYTVLMPVFAKEILHGGSHTFGFLMGAS
                     GLGALMGAVYLASRKSVLGLWKIIPLSAAIFGFGLIAFSLSRIFILSLLLMVAIGLGM
                     ITQMASSNTILQTIADDDKRGRVMSFYSMAFMGTAPFGSILAGWLADLIGVPNTIAIS
                     GVTCIIGALIFVKKLPELKKGVRSIYVKLGIIPEISRGIQTATELTVPPQE"
     gene            2466..3134
                     /gene="nfi"
                     /locus_tag="PLJ75_09925"
     CDS             2466..3134
                     /gene="nfi"
                     /locus_tag="PLJ75_09925"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259813.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HPD05569.1"
                     /translation="MNYHRWECSVSEAIAIQENLATTVITAGKPDTITTVAGIDIALT
                     PQNIGFCIIAVFEYPSLVLIDEVFSSDPITFPYVPGLLSFREGPLVLKAFEKLTEKPD
                     ILIFDGQGIAHPRRFGIASHIGVVLDVPAIGCAKSRLYGNYEEPGITKGDRAYLYDKN
                     NKKIGVVLRTRTNVKPIFVSPGHRVGIDEAADIIMQCTGKYRIPVPTRYAHCRVGEYK
                     RSSL"
     gene            complement(3129..3449)
                     /locus_tag="PLJ75_09930"
     CDS             complement(3129..3449)
                     /locus_tag="PLJ75_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD05570.1"
                     /translation="MPDKFGLSIDTINKLYTVFSKYSEIEKAIIYGSRAKGNYKNGSD
                     IDLTLIGSSDLNLTTLGKIMTGIDDLLLPYTEDLFLYNYLNDPDILDHIRRAGKIFYK
                     KNKL"
     gene            complement(3442..3858)
                     /locus_tag="PLJ75_09935"
     CDS             complement(3442..3858)
                     /locus_tag="PLJ75_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HPD05571.1"
                     /translation="MDQQDICWKQRLSNFSKALDQLTKFIDKGQLNELEEQGLIKVFE
                     YTYELAWNVIKDYFESQGETGLTGSRDAFRMAYKRGIIDDGEIWMDMIKSRILTSHTY
                     DEEIANKISSSIMYYYYPQFRKLHDFLKKLLDKNNA"
     gene            4136..5458
                     /locus_tag="PLJ75_09940"
     CDS             4136..5458
                     /locus_tag="PLJ75_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05572.1"
                     /translation="MEQTTIIIAGLGSAGFSALTTVKRINPKARVVVIDPKEKDIVHP
                     CGLPYALEGIVSYEHLSQDIYLNRMNVEKITGTVQLVDAANHKLIVTSGNNEQLVNFD
                     RAIICTGYKPLVPPIVNIKKYYNNGVYTLSSVQDLLAIKSALHGKQRALVIGAGAIGL
                     EMAYALQVNGLQVTVIEAKDVLPNALDSDMASIVADYCTQNNIALALHAAVNTIEKSN
                     NFIAITNDAQYNADIIIVACGFAPNCDWAIASSLKTSKGGAIVTDNLQVCPDVYAAGD
                     CIECWSVIDKKPLPVKLATSAYKQGVVAAENGMGGNSVYAGTAGTFVTVIGNLEIAAT
                     GFSTDVAKERGFSVIAGKISATVKPEYFPSNERLTMKVLCDIHGKVIGAQAIGHGAAS
                     RINIVSLAINKGLTIDDLLQSEMAYCPAVSEVNDILLKACEFAKRRMK"
     gene            5455..6555
                     /gene="gcvT"
                     /locus_tag="PLJ75_09945"
     CDS             5455..6555
                     /gene="gcvT"
                     /locus_tag="PLJ75_09945"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPD05573.1"
                     /translation="MTAQRTFLYEDHIKLGARMVEFAGWELPVMYTSIIDEHMAVRQS
                     AGIFDVSHMGEIVAKGRGAKAALQRLIPTDMGRLEKGKSMYTCFLNEQGGIIDDLFIF
                     MLSDNEYYLVVNAGTTEKDVEWLTLNCKTNVAIQDVSGVTSKIDLQGPQSYNILKKII
                     KDDRLEGLQRFYFFTGTYKGEPVLVSQTGYTGEFGYELFCINNIISRLWNDILDAGKE
                     YGLKPAGLGARDTLRLESCYSLYGHELSETISPIEGGIGFVVNATSDYIAKDIIEDQK
                     KNGAPREILCLEMMDKTIPRSDYPVVYNKQDIGAITSGGFSPLLKKGIAMALVKRGLV
                     TIHDEVSVGIRGKQSPAKVVARPFYKYNGKRL"
     gene            6625..7017
                     /gene="gcvH"
                     /locus_tag="PLJ75_09950"
     CDS             6625..7017
                     /gene="gcvH"
                     /locus_tag="PLJ75_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008170823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPD05574.1"
                     /translation="MSNIPENLKYTKTHEWIDVQDTYAGVCGITDHAQEMLTDIVFVE
                     LPEVGIEVKAGEQVAVVESVKAVSDVYAPISGRITEVNKLLEDKPELLNSDPYGEGWI
                     YKIDIKDKYELDELMDAAAYAEHVAQGE"
     gene            7020..8339
                     /gene="gcvPA"
                     /locus_tag="PLJ75_09955"
     CDS             7020..8339
                     /gene="gcvPA"
                     /locus_tag="PLJ75_09955"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPD05575.1"
                     /translation="MSFLPNSDDDILEMCSEIGVSSIEDLFCDIPASIKNPTIQLPQA
                     LSEQEALTHILHIGNANEQLCSLVGAGIYNHYVPAVVDHLVSRSEFYTAYTPYQPEVS
                     QGTLTAIFEFQTFICRLTGMDIANASMYDGATALAEAVLMAIRSTGKNRVLCSATVHP
                     HYRDVLSAYLWASDAQLAIVPYKQGLTDYEQLSHMIDDNVACVIVQSPNYFGAIEDVA
                     SIADSAHNHGAVCIATFTEALSLALLKSPGSLGADIACGEGQSFGNYPACGGPLLGIL
                     ACRSKFMRTIPGRLVGKTVDEEGRDAFVMTLQTREQHIRRQRATSNICTNEGLCALRA
                     TIYLSLVGNKLIELARINHALARYCCNRLVALGYERVFTVPFFNEFVIRVNNAKEIRN
                     RLLDNGYALGISIDTYYPELNDCLLLGVTESVSNNDIDNLVELLQTI"
     gene            8339..9772
                     /gene="gcvPB"
                     /locus_tag="PLJ75_09960"
     CDS             8339..9772
                     /gene="gcvPB"
                     /locus_tag="PLJ75_09960"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPD05576.1"
                     /translation="MRVTMQTIFSFHKKGKNKYSIPACDPIQVDEIPQSMLRDDLTLV
                     DVSEVDVVRHYRKLSSLNFGVDNGMYPLGSCTMKYNPKINDVVAMLPQFAMQHPATIE
                     ETGQGALHMMYQLQQILCQITGMHYFSLSPAAGAHGELTSVMIIKKYFETKGEKRNIL
                     LIPDSAHGTNPASVAMCGFEVKEIPSDSDGDVDLTKLEDALNEHVAAMMLTSPNTLGL
                     FDRNILKIAEMLHSKGALFYCDGANLNAVVGKVRPVDMGFDIMHVNLHKTFSTPHGGG
                     GPGAGPIGVVGKLAPFLPVPIIEKADRYYLQYNRPLSIGRIHSFFGNFMVMLRAYTYI
                     MMLGSDGLRSVAEHAVCNANYLRTKLRNHFQVPYNRICMHEFVINDEGLPNGITTNEV
                     AKRLLDYGFHAPTVYFPLIVHGAMMIEPTETEHRDTLDAFADAMLTIKKEAVEDPALL
                     KKAPVTTPVRKVDAVLAARKPVLKWDD"
     gene            9760..>9968
                     /locus_tag="PLJ75_09965"
     CDS             9760..>9968
                     /locus_tag="PLJ75_09965"
                     /inference="COORDINATES: protein motif:HMM:NF028191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPD05577.1"
                     /translation="MGRLTRSIPDWIQCTIPSGPQYKKVKQHIDACGLHTVCIEARCP
                     NVGECFTKGHATFLIMGNVCTRNCK"
BASE COUNT         3012 a         1835 c         2180 g         2941 t
ORIGIN      
        1 cgccaagaat tcgctgcccg gaagttgtag cattgaaatc caccgtatcc accacgccct
       61 gaagcttggc attttcgtgt gcgatagagc gaacagcgtt ggtaagatgt tctcccacat
      121 tggttgtatg gtttgcaaca gcttcccagc gggcatcttt gggaagatag aaacgaacaa
      181 gtttgtgatc ctgagcaagt agcttttcga cagtttcctt tgagccataa tcctgcgaca
      241 atcggtcaag ttcatcctca aacacatccg aaagcctctt aaaaaagatg agcggcagga
      301 tgtagtcctt gtatttaggt gcatcaactg gtccacgaat tttacaggcg gcttcccata
      361 gccagttttc cagcgatttg acatcaagcg tagtgttcat tgcataatcc tcatgttcga
      421 tagttattgt ttataggtgt gttgccatac tatcatgtta tgttttgtat tgtacacaat
      481 tattttgtat acggtatatg gttatatgct attatgtgtg caattttcat cattttctca
      541 ttgcatggga ttttcattga cagtaaaaaa atttttagcc tcaaaaactc ataccgataa
      601 ctatcgccac aatggaagcc gggaagattt atcaccgatc gtatgaagaa aaatgaattg
      661 gggatgaaaa caggtaaggg catctatgcg tggcagggtg gtaaggctat aatagacacc
      721 tcgcacaaaa cagatatcat aaagccggtt gacccgttag cggtacagtt gaatgaagct
      781 atacgcgttt taaaagaaaa ggttgcccgg agtgctgagg atattgacag gggccaggaa
      841 gcaggcatga atcagcccgg cccttttaaa acagcagcgg gtattgacca taaccttata
      901 gcccggcgcc tgcagtggct cagcgcagca tataatctta gctatatcaa tccggagcct
      961 gaaattcttg atggtagctt taaatccatg atataaccat gaggtcagca tggaatacat
     1021 tgaaacacag gtaaaagatg cagtagcttg ttgttatatc aacagaaatg aagtgaagaa
     1081 tgcacttaac agaacgatta tcgatgagct gggtgcaaag atcggctcct tgtggggtca
     1141 gagccctgat tatgattttt atggaacgta tatgggaaaa gaattatgat ttctggtaca
     1201 atcccatcca ttttcagagg atgacgattc aggacatgct acattgcagc gctgcagtaa
     1261 cacctgataa agtagcaaca gacttctatg gcaccaaaat tacatattat gggctacgga
     1321 agatgtctat tacgtttact caatcactgg taaatattgg aataaaaaat gtgatagagt
     1381 caagatgata gttttactcc tgaggaggta cggttaattc agttgcagtt tgaattcccc
     1441 ttgatatttc gggaataata ccaagtttga cgtatattga ccgaacaccc ttcttcagtt
     1501 ctggaagttt ttttacaaag atgagagcac ctataataca tgtcacaccg cttattgcga
     1561 tagtattggg aacacctatt aaatctgcaa gccatcctgc taagatgctg ccaaaaggtg
     1621 ctgtgcccat gaatgccatt gaataaaagc tcataaccct gcctcgttta tcatcatcgg
     1681 caatggtttg taaaatggta ttgcttgatg ccatctgtgt tatcatccca agtccaatgg
     1741 ctaccattaa tagtaaagaa agaataaata tgcgggataa tgaaaaagca atgagcccaa
     1801 aaccaaaaat agctgctgat aaaggaataa tcttccatag ccctaacaca ctttttctgg
     1861 aagcaaggta tactgcaccc atcaaagcgc caagccctga agcccccatg agaaaaccaa
     1921 aagtatgtga tcctccatga agtatttctt tggcaaaaac gggcataagt accgtatagg
     1981 gtgttcccat aaggctaacc aatgcaagga ggagtataac attgtgcatg cctgctaacc
     2041 tacaacgaaa gtgagcacgg tagacaatta tgttgtaaac taattgataa tacgttttta
     2101 tttatctcat cttttttcat atcagtgtac ccatgttgta catagtacaa tactctttgt
     2161 agtcaaagat tttgtttctc tttacattaa ttatagagct attgtcgtcg gttaaccata
     2221 aattatactg ttgtcatgta ttttcattaa aaaattaatc atatcaacca tagatgcaat
     2281 gttataagtt tacatagtat gcaatactat gagcataaca ctatccatgt tattccctgc
     2341 aggtatagca gcttgccgat gccaggtaat accatgagct atcgaacaca ataaaaatat
     2401 gccagcatct tgacttgaca aattttgatg atataactat gtaatgcatg ggggtcagag
     2461 cctttatgaa ttatcaccgc tgggaatgct ccgtttctga agcaatagcc attcaggaaa
     2521 accttgccac aacggtaata acagcaggga agcctgatac cattaccact gttgctggta
     2581 ttgatattgc tctgacccca caaaacattg gtttttgcat catcgcagtt tttgaatacc
     2641 catcgcttgt gctcattgat gaagttttta gctctgaccc cataaccttt ccctatgtgc
     2701 cgggcttgct gtcgttcagg gaaggaccgc tggtgctgaa agcatttgaa aagcttacgg
     2761 aaaaacctga tattcttatt tttgacgggc aggggattgc ccatccccgg cgctttggca
     2821 ttgcaagcca tattggtgta gtacttgatg tgcctgcaat tggctgtgca aaatcacggc
     2881 tctatgggaa ttatgaggag ccggggatta ctaagggtga cagagcatac ctgtatgaca
     2941 aaaacaacaa aaaaattggt gtggtattac gaacgcgtac taacgtaaaa ccaatttttg
     3001 tatcaccggg acatagagta gggattgatg aagcagcaga tattataatg cagtgtacgg
     3061 ggaagtaccg tatacctgtc cccacacgct atgctcattg cagggtagga gaatataagc
     3121 gaagcagttt atagcttatt ttttttgtaa aatattttac cagctcgccg aatatgatcc
     3181 agtatatccg gatcgttgag ataattgtat agaaataaat cttccgtgta ggggagaaga
     3241 agatcatcaa taccagtcat gattttacct aaagtggtaa ggtttaaatc actactgcca
     3301 attaatgtta aatcaatgtc agatccattt ttgtaatttc cttttgctct ggaaccataa
     3361 ataattgctt tttcaatttc tgaatattta gaaaatacag tatataattt gtttattgta
     3421 tcaatactaa gtccaaattt atcaggcatt atttttgtcc aataattttt ttaaaaaatc
     3481 atgaagtttt ctaaattgtg gatagtaata atacattatt gatgaagaaa tcttatttgc
     3541 tatttcctca tcgtaagtat ggctggtaag aatacgactt ttaatcatat ccatccatat
     3601 ctctccatca tcaataatac ctcgtttgta agccatacga aatgcatcgc gactacctgt
     3661 taaacctgtt tcgccctgcg attcaaaata atcctttatt acattccatg ccaattcata
     3721 agtatattca aatactttaa ttaatccctg ttcttctaac tcatttaatt gacctttatc
     3781 aataaatttt gtcaattgat caagagcctt tgaaaaattg cttaatcgtt gtttccagca
     3841 aatatcctgt tgatccattg tatagtacct gtataattgt agtaagagta taaaatacta
     3901 ttgtaccgtt ttatcaatga aatttgataa acatccatta aacactgaaa acaataaaat
     3961 tttttttatt gactatatgc catatatttc ataagtatta aaaaaattat ttgtattcat
     4021 gctttctcca gtagttagtt ttcaggtttt aaaaataata gcttcatact agtgtgtttt
     4081 tataagtgat atatatacta cataggaaga tatagttatt taaagtgtac attacatgga
     4141 acaaacaaca ataattattg ccggtttggg cagtgcgggg ttttctgcac ttacaacggt
     4201 aaagcggatt aatccaaaag cacgagttgt ggtcattgat ccaaaagaaa aggatattgt
     4261 gcatccatgc gggttgccct atgcactgga agggattgtt agctatgaac atctgagtca
     4321 ggatatctat ctcaaccgca tgaatgttga aaagataacg ggaacggttc aattggttga
     4381 tgctgcaaat cataaactta ttgttacatc aggcaacaat gaacaattgg taaactttga
     4441 cagagcaatt atttgtactg gttataagcc tttggtgcca cccattgtta atatcaaaaa
     4501 atattataac aatggtgtgt atacgctctc ttccgttcag gatttgcttg ctatcaaaag
     4561 tgctttacac ggtaaacaaa gggcgctggt tattggagca ggggctattg gtcttgaaat
     4621 ggcatatgct ctacaggtta acggattgca ggtaactgtt attgaagcaa aggatgtgtt
     4681 gcccaatgcg ctggacagtg atatggcatc aatagtggcg gactactgca cacaaaacaa
     4741 tattgctctt gcattacatg ctgctgtcaa taccattgaa aaaagtaata atttcattgc
     4801 tattaccaat gatgcacaat acaatgcaga tatcattatt gttgcctgtg gttttgcacc
     4861 caactgtgat tgggctattg cctcttcact gaaaacatca aaaggtgggg cgatagttac
     4921 tgataatcta caggtttgcc ctgatgtata tgctgcaggt gattgtattg aatgctggtc
     4981 agttattgat aaaaaacctt tgccggtaaa acttgcaaca tcggcataca agcagggtgt
     5041 tgttgctgcc gaaaatggta tgggtggcaa ttcagtttat gcaggtactg ccggtacgtt
     5101 tgttaccgtg ataggcaatc tggagattgc tgcaaccggt ttttcaacag atgtagcaaa
     5161 agaacgtggt ttttccgtga tagcagggaa gataagtgct acagtaaaac cggaatattt
     5221 tccgtccaat gaacgattga ctatgaaggt gctctgtgat atacatggaa aggtgattgg
     5281 agcgcaggca ataggccatg gagcagcttc aaggattaat atagtcagtt tagccatcaa
     5341 caagggatta accattgatg atttattaca gtccgagatg gcatactgtc cggcagtaag
     5401 tgaagttaac gatattttat tgaaagcatg tgaatttgca aaacggagaa tgaaatgacc
     5461 gcacaaagaa catttttata tgaggatcac ataaaattag gtgccaggat ggttgagttt
     5521 gcaggctggg aactgcctgt catgtatact tcaattattg atgagcacat ggctgtgcgt
     5581 caatccgcag gcatttttga tgtgtcacat atgggcgaga ttgttgcaaa agggcgtggt
     5641 gcaaaagctg cattacagag gcttatacca accgatatgg gcaggcttga gaaaggcaaa
     5701 tcaatgtata cctgcttttt aaatgagcag ggtggcatta ttgatgatct ttttatcttc
     5761 atgttatccg acaatgaata ttatcttgtt gttaatgcag gcacaacaga aaaggatgta
     5821 gaatggttaa ccctgaattg taaaactaat gttgcaattc aggatgtttc aggggttacc
     5881 tcaaagattg atcttcaggg cccgcaatca tacaatatct taaaaaagat aattaaggat
     5941 gacaggctgg aaggtttaca gcggttttac ttttttacgg gaacatataa aggcgaacct
     6001 gttctggtgt cacagacagg gtataccggt gagtttggct atgagctttt ttgtatcaac
     6061 aatataattt cacgattgtg gaatgatatc cttgatgccg gaaaagaata tggcttaaaa
     6121 ccagcagggc ttggtgcacg ggatacattg cggttggagt catgctactc attatatgga
     6181 catgaactga gtgaaacgat ttcaccaatt gaaggtggca ttggttttgt tgttaacgca
     6241 acatcagatt acatagcaaa ggatattatt gaagaccaga aaaaaaatgg tgctccgcgt
     6301 gaaatactat gtcttgaaat gatggataaa actatccccc gcagcgatta cccggtagta
     6361 tataataaac aggatatagg cgctataacc agcggtggat tttcaccctt attgaaaaag
     6421 ggaattgcaa tggcgctggt taagcgtggt ttggttacca tacatgatga ggtgtcagta
     6481 ggtatcaggg gtaaacaaag ccctgcaaag gttgttgcac gaccatttta taaatataat
     6541 ggaaaaaggc tttaatatct gtttacaata agatgctgag ataaaccgtg tatgaaaata
     6601 taaatttatt gtaggagcac tataatgagc aatatacctg aaaaccttaa atacacaaaa
     6661 acacatgagt ggattgacgt gcaggatacg tatgccggtg tatgtggtat tactgatcac
     6721 gcgcaggaga tgctcacgga tattgtattt gtagagttgc ctgaggttgg gatagaagtc
     6781 aaagctggtg agcaggtggc tgttgttgaa tcagtaaaag cagtgagtga tgtgtatgca
     6841 ccgataagcg gaagaataac cgaagtaaac aaactgttag aggataagcc ggaactttta
     6901 aacagtgatc cctatggtga aggctggatt tataaaatag atataaagga taagtatgaa
     6961 ctggatgaac tgatggacgc tgctgcttat gcagagcatg tagcccaggg tgaatagcca
     7021 tgagtttttt acccaacagt gacgatgata tccttgaaat gtgtagtgaa attggtgttt
     7081 caagcataga agatcttttt tgtgacattc ctgcttcaat aaaaaatccc actatacaac
     7141 ttcctcaagc gttatccgag caggaagcgc ttacgcatat acttcatata ggcaatgcta
     7201 atgaacaact atgttctctt gttggtgctg gtatctataa tcattatgtt cccgctgttg
     7261 ttgatcattt agtttcgcgc tctgagtttt atactgccta tactccctat cagcccgagg
     7321 taagtcaggg aacgttaact gcaatctttg aatttcaaac atttatatgc aggctcaccg
     7381 gtatggatat tgccaatgca agcatgtatg acggcgcaac agcgttagcc gaagcagtgc
     7441 ttatggctat acgttcaacc ggcaaaaaca gggtgctgtg ctcggcaacg gttcatcccc
     7501 attaccgtga tgttctttct gcctacctgt gggcatcgga tgcgcaattg gcgatagttc
     7561 cctataaaca gggcttaact gattatgaac aactatcgca catgatagat gacaatgttg
     7621 cctgcgttat tgtgcagagc cctaattatt ttggagctat tgaggatgtt gcaagtattg
     7681 cggatagtgc acataaccat ggtgcggtat gtatagcaac atttactgaa gcgttgagtt
     7741 tggcattatt aaaaagcccg ggcagtcttg gtgcagacat tgcctgcggc gaggggcaat
     7801 cgtttggaaa ttatcctgca tgtggaggcc cgcttttagg aattttggcc tgccgcagca
     7861 aatttatgcg caccataccg ggcaggctgg ttgggaaaac agtggatgaa gaaggcaggg
     7921 atgcttttgt tatgacattg caaacacgtg aacagcatat cagacgtcag cgtgcaacat
     7981 caaatatatg caccaatgaa gggctatgcg ctctgcgtgc tactatatat ttaagccttg
     8041 ttggaaataa attgatagag cttgccagaa ttaatcatgc tttagcccgt tactgttgca
     8101 acaggctcgt tgcattagga tatgagcgtg tgtttactgt accatttttc aatgaatttg
     8161 ttatacgggt taacaatgca aaagaaatca gaaacaggtt gcttgataac ggatatgcac
     8221 tgggaatatc cattgatacc tattatcccg agcttaacga ttgccttttg cttggtgtta
     8281 ccgaaagtgt atcaaacaat gatattgaca atctggttga attgttgcaa acaatttgat
     8341 gagggtaact atgcagacaa tattttcatt tcataaaaaa ggtaaaaata aatatagtat
     8401 tccagcctgt gatcccatac aggttgatga aatcccccag tcaatgctac gcgatgattt
     8461 aacattggtt gatgtaagcg aggttgatgt ggtgcgtcat tatcggaaac tttcatcttt
     8521 aaactttggt gttgataatg gtatgtatcc gctgggaagc tgtaccatga agtataatcc
     8581 caagataaat gatgttgtag ccatgctgcc gcagtttgcg atgcagcatc cggctaccat
     8641 tgaggaaaca gggcagggcg cattacatat gatgtatcag ctacaacaga tactgtgcca
     8701 gattaccggg atgcactatt tttcattatc gcctgctgcg ggtgcccatg gcgagcttac
     8761 cagcgtcatg attataaaaa aatattttga aacaaaaggt gaaaagcgca atatactctt
     8821 aattcccgat tcggcgcatg gcaccaatcc cgcttcagtt gccatgtgcg gttttgaggt
     8881 aaaagaaatt ccgtcggaca gtgacgggga tgttgacctt actaaattgg aagatgctct
     8941 caatgaacat gttgccgcaa tgatgctgac aagccccaat actcttgggc tatttgacag
     9001 aaatatacta aaaattgcag agatgttgca cagcaaggga gcgctgtttt actgtgatgg
     9061 cgctaacctc aacgctgtgg tgggtaaggt gcgccctgtt gatatgggtt ttgatatcat
     9121 gcatgttaac cttcataaaa cgttttccac accacacggc ggtggcggtc ctggagcagg
     9181 tcccataggt gttgtgggaa agttagctcc ttttttacct gttccgatta ttgaaaaagc
     9241 cgataggtat tatttgcagt ataacaggcc gttatctatt ggcaggattc atagcttttt
     9301 tggaaatttt atggtaatgc tgcgtgccta tacctatata atgatgcttg gttcagatgg
     9361 tttacgcagt gttgcagaac atgctgtatg caatgcaaat tatttacgaa caaaattacg
     9421 caatcatttt caggtaccgt acaaccgtat ctgcatgcat gaatttgtca tcaatgatga
     9481 aggattgccc aatgggataa ccaccaatga ggtggcaaag cgtttgcttg attatggatt
     9541 ccatgcacca accgtttatt tcccgctcat tgttcatgga gcaatgatga tagagcctac
     9601 ggaaaccgag cacagggata cgcttgatgc atttgctgat gccatgctta ccataaaaaa
     9661 agaagcagtc gaagatcctg cactattaaa aaaagcacct gttaccacgc cagtgcgaaa
     9721 ggttgacgca gtcctggcag cacgcaaacc ggtattaaaa tgggacgact gactcgatca
     9781 atacctgatt ggatacaatg taccataccg tcaggtccgc agtataaaaa agtaaaacaa
     9841 cacattgacg cttgtggtct tcatacggtg tgcattgagg ctcgctgccc caatgtgggt
     9901 gaatgtttta caaaaggaca tgctaccttt ctcattatgg gcaatgtgtg tacgcgcaat
     9961 tgcaaata
//
LOCUS       DAOOJH010000085         9967 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6036_length_9967_cov_9.558717, whole genome shotgun sequence.
ACCESSION   DAOOJH010000085 DAOOJH010000000
VERSION     DAOOJH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6036_length_9967_cov_9.558717"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1568)
                     /gene="flgE"
                     /locus_tag="PLJ75_09970"
     CDS             complement(198..1568)
                     /gene="flgE"
                     /locus_tag="PLJ75_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HPD05578.1"
                     /translation="MMRSLFAGVSGLKNHQTRMDVIGNNISNVNTYGFKYSRVTFQDM
                     LSQTISGAAKPEENRGGINPRQVGLGMNIAAIDKIFTQGSLQTTGVNTDMAISGDGFF
                     IVAEGQKHLYTRAGTFALDKDGTLVNPANGLRVQGWMATTNAAGEKVINTSGTVEDII
                     VPIYGKVPAKETNYVKYKCNIDSKLPIVPNDANAIMRTSAGVTTNIDIYDKLGNPHRL
                     TLNFWKSGVNEWTASAAITDTELVTLDVPNGGNQPNQINGSSRVVLKFSPEGRIMSVA
                     DANSPDEINQGKLEVNLSYRVAGDPTQRNVRLDFGSAGLVEGITQFSSPSTTKAVEQD
                     GYTMGYMESFTVDNSGIITAVYSNGTKQTVGQVATAVFTNPQGLIAAGDNMFELSNNS
                     GLPNIGPAGEAGRGTIVAGTLEMSNVDLSEQFTDMIITQRGFQANSRTITTSDQMLQE
                     LINLKR"
     gene            complement(1588..2196)
                     /locus_tag="PLJ75_09975"
     CDS             complement(1588..2196)
                     /locus_tag="PLJ75_09975"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HPD05579.1"
                     /translation="MPEPLRMSGEDVAKAQLQANEVNATLKAKKGKLPAELDKDSFLK
                     LLVAELRHQDPTQPMNDREFISQMAQFSSLEQMSNMNQAIERLVLRSQSGEAFSLIGK
                     EVEAVDFVSQQLVRGVASGVVYRQDGVKIIVGSTEVSLNDIHAVYMPKNNLTNENKKT
                     QSNEITEKTNNAVNEQTKGMQQYQMNNTFVRGSSSTIQPLRN"
     gene            complement(2208..3590)
                     /locus_tag="PLJ75_09980"
     CDS             complement(2208..3590)
                     /locus_tag="PLJ75_09980"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPD05580.1"
                     /translation="MVKPVILDFDIVTHNQNSARQSEPEQSFISFLDGILQKKEVEET
                     VNSKNVITEFEESNHTKNDNQDKYFADMANKNELSTEKAAKHVSSFNDRRLEKEKADK
                     EKLQLHNEALSQFLTISNSNTINSRATLTKNKDNPVRIPYRDMNISPGRLLHGLDTSF
                     AKRNNTSINDLVSTNTLSGTKKGNSQSLHKVIENMQKIINDEMVNNSKRGDIKKLIAN
                     LVTIQKADPKTMQQGLIARQRLEYNELSNTKKSEKKNSGVTQKFVDNNAPLKNAKNHV
                     TVFINAVRHKEAHEDLPKTKDVKASTAEQHETFNNVNARNGVASQSSVQNVAHEKVIN
                     NSDLMQLLQKARIMQDGEKTNLSLKMYPESLGRLHIQLGLEQGIVNGRFTVESEQARQ
                     QLMQQLDVVRYELEQSGVHVGGFEVHVRDQRQTEIFQHAEAIPLTQNAEYEEQMSRYS
                     YHDGMLDIII"
     gene            4241..5506
                     /locus_tag="PLJ75_09985"
     CDS             4241..5506
                     /locus_tag="PLJ75_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPD05581.1"
                     /translation="MEAIESLPNTENQEPPGCSASNTDEPPSSAFTNSNVVTILNKQI
                     VPRSINAKSRIFRKLYYPAVNDSQWNDWRWQIANRIRTFEHLSSFIELSPDEIMALTG
                     RQYQLPMAITPYYASLIDRNNYGDPIRRCVIPTINELHHSTGESEDPLAEDNDTPVQG
                     LVHRYPDRVLFLATDFCSTYCRYCTRSRIVGRSNCNLSTSHWEGAIRYIAQHSEVRDV
                     LISGGDPLTMNDSMIEYLLSRLRAIPHVEIIRIGTKVPAVLPQRITHTLVSMLRKYHP
                     LWINIHFTHPDELTPETMKACNRLANAGIPLGSQTVLLKGINDTVETLKKLYHLLLQC
                     RVRPYYLYQCDPIVGSAHFRTSVETGIAMIQGLRGHTTGLAIPHYVIDAPGGGGKIPL
                     LPDYFQGIDGNTTVLKNYEHKYFYYPNTQ"
     gene            5503..6531
                     /locus_tag="PLJ75_09990"
     CDS             5503..6531
                     /locus_tag="PLJ75_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019528413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPD05582.1"
                     /translation="MTDSGMRVGLTYDLRDDYRASGYDEEAIAEFDSIETINAIDTTL
                     GNLGFFTDRIGNVKHLIKRLADGQRWDIVFNIAEGMFGISREAQIPALLDAYQIPYTF
                     SDPLVLSLTLNKAMTKQIISYNGIPTPASMLITNINDCNRCTLTYPLFAKPYAEGTGK
                     GITASSVIHDRLELTNTCFHLLKKFNQPVLVEEYLPGREFTAGILGNGFNARSAGIIE
                     IILLDNAEKNVYSFANKEQCEQLVEYRFAEDAIAKKAVDVALRAWQVLGCLDAGRVDL
                     KCDSNGNPQFLEVNPLAGLHPSHSDLPIICTKAGISYTRLIANIMYNALTRYNLLDSA
                     PEKLYSSI"
     gene            6656..7642
                     /locus_tag="PLJ75_09995"
     CDS             6656..7642
                     /locus_tag="PLJ75_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD05583.1"
                     /translation="MEIVFVHQFIHENAPVDEKDVLEQRDELAEIFINMGYAISSRSI
                     QSQLDLILLHELANKDCIVFNLCESLFGRDDLLFLVPFMLEHYNIPFTGSGSMALALS
                     CHKLESKSLMINNFIPTPKYITSSTPYSNNINGTYIIKPYNTHASMHITQDSVIGVHS
                     KHDFDLLLDRLKTSHALFAEEYIDGREFNISLMQTDEGVLILPHAEIIFENYPPNMYK
                     IVDYDAKWAHDSFAYEHTIRNFYFPEDDSPLLNEIDRLSLKVWELFSLNGYARIDYRI
                     RGNEIFILEVNANPCISIYSGFMAAVKQYGLSFQDALDIILKHPIKTNKINI"
     gene            7644..8141
                     /locus_tag="PLJ75_10000"
     CDS             7644..8141
                     /locus_tag="PLJ75_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD05584.1"
                     /translation="MKQINNTIVYRDYVIASDVVVIPAILDATHFFYQEEIEIALELL
                     NEKINCGDSSTYQFIVAECDNTVIGYICYGKIPLTQSSYDVYWIAVHPDFQGNGIGYT
                     LLLRAEETIKKASGKNLFIETSGRKLYQPTHSFYRKSGYTISAVFTDFYADGDDKLVF
                     YKKLI"
     gene            8219..8452
                     /locus_tag="PLJ75_10005"
     CDS             8219..8452
                     /locus_tag="PLJ75_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HPD05585.1"
                     /translation="MECTCGGMLTEGRSSYSMSGEYYYIVIEDIPAYKCSRCGKILFQ
                     DDVVDKIKALLKRVDRDTKEIITGTPSVHTYDY"
     gene            8482..9921
                     /gene="gltX"
                     /locus_tag="PLJ75_10010"
     CDS             8482..9921
                     /gene="gltX"
                     /locus_tag="PLJ75_10010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPD05586.1"
                     /translation="MMRVRFAPSPTGFLHVGNARTAVLNYLVKKKNNAAYILRIEDTD
                     TQRSTMESEQSILNDLKWLGIQWDEGPDCGGKFGPYRQSERFDIYRQFTEKLLITGNA
                     YHCYCTQEELDAMRAKAAEHNKPFVYPGTCRHLSLEQKLAYEHQGRKPTVRFRVKDGI
                     SIVVNDHIKGKVVFSSENIGGDFIIVRSDGIPVYNYIVIIDDTLMNITHVIRGEDHLP
                     NTPKQMLVAMALDLPAPEYAHMPLLLGPDRTKLSKRHGITSVSIYREQGYLPQALFNY
                     LALLGWTTQSGEEIVPIDELVEQFELTKIGKSPAIFDFQKLKWMNSEYLKNLPSEQVY
                     SLFYPYLEKAGYLTHDTEWLNSVIDTIRGNCQIASDIVSEIAIFFGDTVEPDHEAGEL
                     LSQQESKAVIMTAYELITSNEVNEHNYHQLPNVIKQKTGLKGKMLFMPFRAMITGRLH
                     GPELDKIIPLLGYQRFKKRIENCYQRFIN"
BASE COUNT         2933 a         2008 c         1799 g         3227 t
ORIGIN      
        1 atgctatggg ggtttttcaa aacccccatt tactctattc tttaccgctt cagatgtacc
       61 aattcttgaa gcatctctat gctatggggg tttttcaaaa cccccattta ctctattctt
      121 taccgcttca gatgtaccaa ttcttgaagc atctctatgc tatgggggtt tttcaaaacc
      181 cccatttact ctattcttta ccgcttcaga tttatcaatt cttgaagcat ctgatcagat
      241 gttgttattg ttcttgaatt tgcctgaaat cctcgctggg taataatcat atcagtaaac
      301 tgttcggata agtctacatt agacatttcc agtgtcccag ctactatcgt acccctgcca
      361 gcttcgccgg caggtccaat atttggcaac cctgagttat tggacaactc aaacatatta
      421 tcacctgctg caatcaaacc ctgcggattg gtaaacacag ccgtagctac ctgcccaacc
      481 gtttgctttg ttccgtttga atatactgca gttataattc ctgagttatc aaccgtaaac
      541 gattccatat accccatggt gtaaccatcc tgctcaacag ctttagtggt tgatggcgaa
      601 gaaaactgtg taatgccctc cacaagccct gcactaccaa agtcaagcct tacattgcgc
      661 tgtgttgggt cacctgctac cctatagctt aaatttacct caagctttcc ctgatttatt
      721 tcatcagggc tattagcatc agccactgac atgatgcgcc cctcaggtga aaatttaagc
      781 accaccctgg aagagccatt aatctgattt ggctggttgc caccattagg tacatctaac
      841 gtaacaagct ctgtatcggt aattgcagcc gaagcagtcc actcatttac tccgcttttc
      901 cagaaattga gcgttaatcg gtgaggattc cccaatttgt catagatatc aatattggtt
      961 gtaactcctg cagatgttcg cataatagca ttggcatcat tgggtacaat tggtaatttt
     1021 gaatctatgt tacatttgta ttttacatag ttggtttctt ttgcaggtac ttttccataa
     1081 atcggaacaa tgatatcttc taccgttcca gacgtattga taactttttc accggcagca
     1141 ttggttgtag ccatccatcc ctgcactctt aacccgtttg caggattaac cagcgtccca
     1201 tctttatcaa gagcaaatgt gccagccctt gtatatagat gtttttgacc ctctgccaca
     1261 ataaaaaaac catcacccga tattgccata tcagtattga caccggttgt ctgcaaactt
     1321 ccctgagtaa aaattttatc aatagctgca atattcatac ccagcccaac ctgacgcgga
     1381 tttataccac cgcggttttc ctcaggtttt gcagcaccag aaattgtttg tgaaagcata
     1441 tcctggaatg tgactctgct gtatttgaat ccataggtat taacattgga aatgttgttg
     1501 cctataacat ccatccttgt ctgatgattt ttaagtccgg aaacacctgc aaaaagtgat
     1561 cgcatcatat cgcaaaactc ctgttatcta atttcgtaat ggttgtatag ttgatgaact
     1621 tcctctgaca aatgtattgt tcatctgata ctgctgcatg cctttggttt gttcattcac
     1681 tgcattattt gttttctcag ttatttcatt gctttgtgtc tttttatttt catttgtaag
     1741 attgtttttg ggcatgtata ctgcatgtat atcattaagg gaaacttcag tagaaccaac
     1801 aataattttc actccatcct gcctgtaaac aacaccactt gcaacaccac gaaccagttg
     1861 ctgtgaaaca aaatcaactg cctccacctc tttcccgatg agcgaaaacg cctctccgga
     1921 ctgtgaccgc agtaccaaac gctcaattgc ctggttcata ttactcatct gttccagtga
     1981 agagaactgt gccatctgac ttataaattc tcggtcattc attggctgtg ttgggtcctg
     2041 atgccgtagt tcagcaacaa gcaacttcaa aaatgaatcc ttatcaagct cggcaggcaa
     2101 tttacccttt ttagccttca gtgtagcatt aacctcatta gcctgcagtt gcgcctttgc
     2161 aacatcttcg cctgacattc gtaatggttc cggcattgtc attctcctta aattattata
     2221 tccagcatac catcatgata actatatcgg ctcatctgtt cttcatattc agcattttgt
     2281 gttaaaggaa tagcttcagc atgttgaaaa atttcggttt gtctctgatc cctcacatgt
     2341 acctcaaaac caccaacatg gacaccactt tgctccagtt cataccgtac aacgtcaagc
     2401 tgctgcatca gttgttgtcg cgcctgctcg ctctcaacgg taaaacgccc gtttacaatt
     2461 ccctgctcca atcctaactg aatatgtaat cgtcctaaac tttcaggata cattttcaat
     2521 gacagattgg tcttctcacc atcctgcatt atcctggctt tttgtaaaag ctgcatcaaa
     2581 tcagaattgt ttatgacttt ttcatgcgct acgttttgca ctgagctttg ggaagcaacc
     2641 ccatttcgtg cgtttacatt attaaatgtt tcatgttgtt ctgcagttga tgcttttaca
     2701 tcctttgttt ttggcaaatc ttcatgtgct tctttatggc gtacagcatt gataaaaact
     2761 gtaacatgat ttttagcatt ttttaaaggt gcattattat ctacaaattt ttgtgtaaca
     2821 ccggagtttt ttttctcaga ttttttagtg tttgatagtt cattatattc taatcgctgc
     2881 cttgcaatga gcccttgttg catggtttta ggatcagctt tttgaatcgt tacgagattc
     2941 gcaatgagct tttttatatc acctctttta ctattgttta ccatttcatc attaattatt
     3001 ttctgcatat tctcaatcac cttatgcagt gattgcgaat tacctttttt agtacctgat
     3061 agagtatttg tgctaacaag gtcattgata ctggtattgt tacgttttgc aaatgaggta
     3121 tctaatccat ggagtaatct tcctggtgat atattcatat ccctataagg tatccttaca
     3181 ggattatcct tattttttgt gagtgtcgcc ctgctattta tcgtattgct atttgaaatt
     3241 gttaaaaatt gggaaagtgc ttcattgtgc aattgtagct tttccttatc agccttttct
     3301 ttttccaaac gtctatcatt aaaactggat acatgtttgg cagctttctc agtgcttaat
     3361 tcatttttat ttgccatatc agcaaaatat ttatcctggt tgtcattttt tgtatggtta
     3421 gattcttcaa attcagtaat gacatttttg ctgttgacag tttcttctac ctcttttttt
     3481 tgtaaaatcc catctaaaaa tgatataaac gattgctcag gctcagactg ccgtgcacta
     3541 ttctgattgt gtgttacaat gtcaaaatcc aatataacag gttttaccat atatcaatcc
     3601 tgtaaaatat atatgtcgta tcattaatat cgaatgtgtt atatttttta atgaattaat
     3661 tttaattttt ttagaagctt cttcccttta ccgggtaatg ataacaaata aatatataaa
     3721 aatttttata aatattttaa aaaatattgt tgcctatatt gatatgtgct ataaattata
     3781 ctgtaacttc tgcgctatta cctgcaatgt ttaaatgcag gcaagtagca gtgtaatttg
     3841 gaatgcaata cttccataat tgccgatcct ccatttttta cggatcccag agttaagtcc
     3901 ttacaatggg agacctcttg gacgctcatc cctgtgaaag cagggatgct ttttttattg
     3961 cctttattta ttataatacc catcagaagc tgtaaaaaat attaattttt tttggtaaat
     4021 tttgtttttt tctttacaag ctatatattt gttctttatt aagttttatt tttgtaacaa
     4081 tacgccgatg gaaataatat gaattttttt atagaaaaaa ttttttattt ttttgtgaca
     4141 taatacggta ttttacttga caaattactt tttttataat gggaagaact aagtgaattt
     4201 ttatacatat aatcacatga ataagtgagg gaacatataa atggaagcaa ttgaatcact
     4261 gccgaatacc gaaaatcagg agcctccggg ttgttccgcc tcaaatactg atgagcctcc
     4321 atcgtcagct tttaccaatt caaacgttgt aaccatctta aataagcaaa ttgttcccag
     4381 gtcaatcaat gcaaagtcaa gaatatttag aaaactgtat tatcctgctg ttaatgactc
     4441 ccagtggaat gattggcgct ggcagatagc taatagaatt cgcacctttg aacatttatc
     4501 aagttttata gagctatcgc cagatgaaat tatggcatta acaggcaggc aatatcagtt
     4561 gccaatggca ataacaccgt attatgcaag ccttatcgat aggaataatt atggcgaccc
     4621 catacgccgt tgtgttatac ctacaatcaa tgaacttcac catagtacag gagaatcaga
     4681 ggaccctctt gcagaagata atgatacccc cgtccaagga ttggtgcatc ggtaccccga
     4741 tagagtattg tttttagcta ctgacttttg ttcaacatat tgccgttatt gtacccgttc
     4801 acggattgtt gggcgttcaa actgcaatct ttccacttcg cattgggaag gtgcaatcag
     4861 atatattgca caacattcag aggtacgcga tgttttaatc tctgggggcg accctttgac
     4921 aatgaacgac tcaatgattg aatacctcct atcacgtctt agagccatac cccatgttga
     4981 aattattaga attggcacca aggttcctgc tgtgttgcct caacgtatta cccatacgct
     5041 ggtaagcatg ctacggaaat accatccctt atggataaat attcacttta cccatcctga
     5101 tgaacttaca ccagaaacaa tgaaagcctg caacaggctt gcaaatgccg gtataccttt
     5161 aggcagtcaa acagtactcc tgaaaggtat caacgatact gttgaaacac tcaaaaaact
     5221 ttaccatctg ctgttgcaat gtcgtgtccg cccttattat ctgtatcaat gcgatcctat
     5281 agttgggtca gcccacttcc gtacatctgt tgaaacggga atcgcaatga ttcaaggatt
     5341 acgcgggcat acaacaggat tggctatccc gcattatgta attgatgctc ctggcggcgg
     5401 tgggaaaata ccccttttac ctgactactt tcagggtatc gatggaaata ccactgtttt
     5461 aaaaaactat gaacacaaat atttctatta tcctaatact caatgactga tagtggcatg
     5521 cgtgttgggc taacctacga tcttcgtgat gactacagag catcaggata tgatgaagaa
     5581 gctattgctg agtttgatag catagaaaca atcaatgcaa tcgatacaac tcttgggaat
     5641 ctgggctttt ttactgatag gataggcaat gtcaaacatc ttataaagcg attagctgat
     5701 ggacagcgct gggatatagt attcaacata gctgaaggga tgtttggcat tagcagagaa
     5761 gctcagatac ctgctcttct ggatgcctat cagatcccat acaccttttc tgatccgctt
     5821 gttctatcgc ttaccctgaa caaagccatg acaaaacaga taatcagcta caatggtata
     5881 ccaacaccag catctatgct cattacaaat attaatgatt gtaacagatg cacacttacc
     5941 tatccgttat ttgcaaaacc gtatgctgaa gggacaggga aagggattac tgcatcttca
     6001 gtgattcatg ataggctgga attaacaaat acctgctttc atcttctaaa aaaatttaat
     6061 cagcctgttc tggtagaaga ataccttccg gggagggaat ttaccgcggg gatattgggg
     6121 aatggtttta atgcccgatc tgcaggaata attgaaataa ttttattgga taatgccgaa
     6181 aaaaacgtct attcgtttgc caataaagag caatgcgagc aactggtaga gtacagattt
     6241 gcagaagatg ctattgcaaa aaaggctgta gatgttgcat tgcgcgcatg gcaggtttta
     6301 ggttgtttag atgccggacg tgttgattta aaatgcgaca gcaacgggaa cccccaattt
     6361 ttagaagtaa atccattagc agggcttcac ccatcacatt ctgatctgcc cattatatgc
     6421 acaaaagctg gcatatcata taccaggcta attgcaaata ttatgtacaa tgctcttacc
     6481 cgttataatc ttctggatag tgcacctgaa aaattatatt catctatata aaaataatgg
     6541 ttgcacaatt ttcattacct aaaaaattta ttgtatgctg ttatgctttt tatatttatt
     6601 caatcgtagt atcagatcaa tccttatata atattatact aaggcagtgg gttacatgga
     6661 gatagttttt gttcatcaat ttattcatga aaatgctccg gtagatgaaa aggatgtcct
     6721 tgagcagcgc gatgaattag cagaaatttt tatcaatatg ggatatgcca taagctcgcg
     6781 cagcatacaa tcacagttag atcttatctt acttcatgaa ctggcaaaca aagattgcat
     6841 tgtatttaat ctttgcgaaa gcctatttgg aagggatgat cttttgttcc ttgtaccttt
     6901 tatgttagaa cattacaaca ttccctttac tggttcaggt tcaatggcat tggcgctcag
     6961 ctgtcataaa cttgaaagta aatcattgat gatcaataat ttcataccaa cgcctaagta
     7021 tataacatca tcaacaccat acagtaataa cattaatgga acgtatatca ttaagcccta
     7081 taatacccat gcatcaatgc atattacgca ggattcagtt ataggtgtac actcaaaaca
     7141 tgattttgat ttgcttctag acaggctcaa gacatcgcac gctctttttg ctgaagaata
     7201 cattgatgga agagaattta atatttcact catgcaaacc gatgaaggag ttttgatact
     7261 tcctcatgct gaaattattt ttgaaaacta tcctccgaac atgtataaga ttgttgatta
     7321 tgatgcgaag tgggcacatg attcttttgc ttatgaacat actatccgca atttttattt
     7381 tcctgaagat gatagccctt tactaaatga gattgacagg ctatccttaa aagtatggga
     7441 attattttca ttaaatggat atgcacgcat agattacaga atcagaggaa atgaaatatt
     7501 tatattagaa gtaaatgcaa atccctgtat atcaatctac agcggcttta tggctgctgt
     7561 aaagcaatat ggtttatctt ttcaggatgc gcttgatata atactaaaac accctattaa
     7621 aactaataaa attaacattt aacatgaaac aaatcaacaa tacaattgtt tatcgtgatt
     7681 atgttattgc atcagatgtt gttgtaatac cggcaattct tgatgctact cacttttttt
     7741 atcaggaaga aattgaaatt gctttagagc tattgaacga gaagatcaat tgtggcgata
     7801 gttccacgta tcagtttata gttgccgaat gtgacaacac cgttatagga tatatctgct
     7861 atggcaaaat cccgcttact caatcttcat acgatgttta ctggatagct gtccatcctg
     7921 attttcaggg gaatggcatt ggttatacac ttttattacg tgctgaagaa acaataaaaa
     7981 aagcatcggg caaaaaccta tttatagaga cctccggaag aaaactttac caaccaacgc
     8041 atagttttta tcgcaaatca ggctatacta tctctgcagt atttaccgac ttttacgccg
     8101 atggcgatga caagcttgtc ttctacaaaa aattaatata attttctttg tttttttatt
     8161 gtaattagta tcttgacata tatacctttt tttaattttc tgtatatacg aggttagcat
     8221 ggaatgcacg tgtggtggta tgctcacaga aggtagaagt tcatatagca tgagcggcga
     8281 gtattattat atcgttattg aggatattcc agcgtataaa tgttcacgat gtggtaaaat
     8341 attatttcag gatgatgttg ttgataaaat aaaggcatta ttaaaacgcg ttgatcgtga
     8401 tacaaaagag attatcaccg gcaccccttc ggtgcatacc tatgattatt aatgtgtata
     8461 ctatagtaac aggagaacat aatgatgcgc gtacgatttg ctccaagtcc aaccggattt
     8521 ttacatgtag ggaatgcacg aactgctgtt cttaattatc ttgttaagaa aaaaaacaac
     8581 gctgcctata tactacgtat tgaagatact gacacacagc ggagcaccat ggaatctgaa
     8641 caatccattc tcaatgattt aaaatggctg ggcatacaat gggacgaagg ccccgactgc
     8701 ggtggcaaat ttggccctta tcgtcaatcc gagcgatttg atatttaccg ccagtttacg
     8761 gaaaaattgc ttataaccgg taatgcctat cattgctact gtactcaaga agagctggat
     8821 gccatgcgcg caaaggctgc agagcacaat aaaccatttg tatatccggg aacctgccgc
     8881 catttatcat tagagcaaaa gcttgcctat gaacatcagg gcagaaagcc aacagttcgc
     8941 ttcagggtta aagacggtat aagcatagtt gttaacgatc atataaaagg taaggttgtc
     9001 ttcagcagtg aaaatatagg aggcgatttt attattgtgc gttcagatgg tatcccggta
     9061 tataattaca ttgttattat tgacgataca ctgatgaata taacccacgt catccgtggc
     9121 gaggaccatc tgcccaatac accaaagcaa atgctcgttg ccatggctct tgatcttcca
     9181 gcccctgaat atgctcatat gccccttttg cttggtcctg accgcacaaa attatcaaag
     9241 cggcatggca taacctccgt ttcaatatac cgtgagcagg ggtatctgcc ccaggccctt
     9301 tttaactatc ttgcactgct ggggtggaca acccaatcag gtgaggagat agtccctata
     9361 gatgagctcg tggaacaatt tgaattaaca aaaattggta aaagcccggc tatctttgat
     9421 ttccaaaaac tcaagtggat gaattctgaa tatctgaaaa accttccctc agaacaggtc
     9481 tattcactat tttatcctta cctcgaaaaa gcaggatatt taacccatga cacagaatgg
     9541 ctcaacagcg ttattgacac catcagaggg aattgccaga tagcttcgga tatagtcagt
     9601 gaaatagcaa tattctttgg tgacacagtt gaacctgacc atgaagcagg tgagctgtta
     9661 tcgcagcagg aatcaaaggc tgttataatg acagcctatg aacttatcac cagtaatgaa
     9721 gttaatgaac ataactatca ccaattgccg aatgttataa agcaaaaaac cggattaaag
     9781 ggtaagatgc tgtttatgcc attcagggca atgattaccg gcaggctcca tgggcctgaa
     9841 cttgacaaaa taataccact attaggatat cagcgtttta aaaaacgcat tgaaaactgt
     9901 tatcagcgat ttatcaatta aagacttacc tttatggtgt acgatagtta tatgaaaaca
     9961 acaggcg
//
LOCUS       DAOOJH010000086         9902 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6091_length_9902_cov_9.269828, whole genome shotgun sequence.
ACCESSION   DAOOJH010000086 DAOOJH010000000
VERSION     DAOOJH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6091_length_9902_cov_9.269828"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PLJ75_10015"
     CDS             complement(<1..1703)
                     /locus_tag="PLJ75_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPD05587.1"
                     /translation="MDDVVRILEDIINDFDVNKFKNFFYKKTNDFISQDKPLDGCNDE
                     LFDESTQIGTFKVNDVHEFIVIAAKVNKELTQKSGKKAQYTFAKKILKQMIQYTGGFF
                     IFYDSNGNFRFSFIYSIPRPDGKQDWSNFRRYTYYVSKDQTNRTFLQQMQAASFESLE
                     KIIDAFSVEKVTKEFYRNIVELYYTLINNYNNKGLQRFPKEDQNEKEEFAIRLLGRLL
                     FCWFLKMKKDGSKDSLIPQKYLSSELVKNFNGDYYHEYVEPLFFQILNKPLKERLEKY
                     KTKSYSLIPFLNGGLFEPHDSDYYPVTAINEMGISPNINILTISNDWFANLFEVFERY
                     NFTIDENTLVDTEVSIDPEMLGKIFENLLAEYNPETKENARKATGSYYTPREIVDYMV
                     DESLKNYLCEKTKLSRDIIEELLKHENFEAAANLNEHDKEAIIDAIDKIKVIDPACGS
                     GAFPMGILHKLQVILENVDPQSKIWFKKKIGQIVDPLLQKELQIKLEEENWDYIHKLG
                     LIQHSIYGVDIQPIAVEISKLRFFLSLIVDEKIDDEKQNRGIIPLPNLEFKFVAANTL
                     IG"
     gene            complement(1731..5108)
                     /locus_tag="PLJ75_10020"
     CDS             complement(1731..5108)
                     /locus_tag="PLJ75_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD05588.1"
                     /translation="MSNFITNQPTKNLLHRVRDLIAHSKELKFLVGFFYFSGIKDLYT
                     SLQNNADVVVKICVGLNVDTLNYCIVECGNDANVSNKDVINRYFESITKSINSEEFDN
                     KDFYEQIAYFIQLIKNERLIIRKTRQPNHAKLYLFTLSTEQVKQKILITGSSNLTRAG
                     LIGQHEFNVEITDYGIEEAERYFDTIWDESVAITENDACKKELINLLQHQTLIKEITP
                     FEAYCLVLKSYLDTYEIRTASEAINRILISNNYVPYRYQLDAITQAIAIIEKHNGVIL
                     ADVVGLGKTIIACAIAFQLKKRGIVICPPGLIGNDNKDMGWKKYLEDFRLFDWEVRSS
                     GKLEEALEFVNRCKDIEVVIVDEAHRFRNQDTRSYELLKNICRDKIVILLTATPFNNR
                     PSDIFSLIKLFVTPRKSTITLENNLELLFSMYKSKFDKLSYIKRYHDSKDREKRNRAT
                     TFYKYLFGTENIDIRRVNAETHKLAKQIRDVIEPVTIRRNRIDLQKNPYYKDEVQHLS
                     IVKDPIEWFYELTPKQLQFYDTIIEHYFVEDDVEAFKGSIYRPFIYEVGELEINDDND
                     VNEGLLEKNREYQQQKNLFNFMRRLLVKRFESSFGAFKESIKNFKRIHEHVLQFIEKT
                     GGGDPLKGEFILDRQLLEKIYDMDVDEIEQYLQEYETNLQKDVLPKKYKRYKIKTFKY
                     KEKFIDDIKHDIKLFTSILEQLDALDLVNNDPKLDCLIKNVANEFYQNKEKDKNKRKI
                     VIFSEFLDTVCYLKDTLNNKFNNRVLVISGDLSDSKIKEIYKNFDASYQDQKDDYDIL
                     LCTDKLSEGFNLNRAGMIVNYDIPWNPVRVIQRLGRINRISKKVFDELYIVNFFPTEK
                     GAEIVKSREIAQNKMFMIHNTLGEDAKIFDEDEEPTPAELYHRLQINPETLEAESFYT
                     RVLNVFESIKAQYPEIIKNIESTPHRVKVSKKSTYDELLVFFKKNRLFVMNYDYSDGS
                     IVETTLEEVVDRIACNIDEKALPIDERFWGAYEKVKVHKDKSSLPKSVLSIQDRAKNK
                     LQFILSNECPEALQPYIPFLKQLKEDLIDYGTLSDYTLRRIANFNIDYNDATKLQELV
                     NEIAALQQELGNDYLHKIKAKEDGLQKEIIIAIENKI"
     gene            complement(5249..5923)
                     /locus_tag="PLJ75_10025"
     CDS             complement(5249..5923)
                     /locus_tag="PLJ75_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05589.1"
                     /translation="MKAKSMVIMLLFFAIGCAHYEVMVSSINNNETAGNKVFVAPGMK
                     DVAINDLQFQEFSNYIKKVLIKKGFAIVNTIDEADQVVMLVYAISGPQNYTANVPLWG
                     PTGIESSTTYGNISSSGSYYGNTYYNQTYGITGVAQVKRTFYSRTIILIAFDWKLFKT
                     KKVEKQLWKTDIVSIGSSNDLRYVFPYMVVAAEQYIGTNTRHAVTVKINEYDRRVKEY
                     IGYDDK"
     gene            complement(6001..6957)
                     /locus_tag="PLJ75_10030"
     CDS             complement(6001..6957)
                     /locus_tag="PLJ75_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPD05590.1"
                     /translation="MSQNERLQYIDTSIREKGVVTVKEVVEKFEVSPRTVKRDLEYFR
                     DRINAPIVYDVYKKGYLYSLPFDYFSYRNERMLLFYLFLMKLIESPTHFPFVDKAIPD
                     LIKRNILAHYLPLMSRITYEIDEWEQLDITLFSKLFSTLRNDNCIVIKYADVTGKTTT
                     RTIAPEHFIHYEGRWYVIAYDETKKGIRTFLLSRIQKAEVVNKKRTRKINDDELQKFI
                     KSTYGIYKGSGGKLVQVRFYQPVCNIVKKQVWHKEQTMKEGKHEQYGAYVELQLPVHH
                     PQELLGKVLRYGKYAEIVAPSSIRTMWLEAIKEAYVRFVKSK"
     gene            7414..9093
                     /gene="ilvB"
                     /locus_tag="PLJ75_10035"
     CDS             7414..9093
                     /gene="ilvB"
                     /locus_tag="PLJ75_10035"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPD05591.1"
                     /translation="MKITGAQIIIESLIREKVEVMFGYPGGVVIPIFHHLFNAPFKFI
                     LSRHEQGAIHAADGYARATGKPGVVIATSGPGATNLVTGIATANMDSIPLVIFTGQVS
                     TEVIGNDAFQEADVTGITRPITKHNYLVKDINELAKIIKEAFYIATTGRPGPVLIDVP
                     VNISNAETEFHYPETVHLRSYKPVIHGHSGQIQKACKLIEQSKRPVVYAGGGVISSNA
                     SDELRTFIKKTGIPITTTLLGLGAYPENDKLSLKMLGMHGTYYANHAVHQSDLLIAVG
                     ARFDDRVTGKISEFIPHAKVIHIDIDPSSVSKNVAVDVPIVGDAKHVLTEINKTVKAP
                     KIDEWVTHVMKMKEENPLTYKDSDVTIKPQYVIEKIYEISNGDAIICTEVGQNQMWAS
                     QFFDYTQPRTFISSGGLGTMGFGFPASIGAQLGRPDKRVIDIAGDGSIQMNIQELIVA
                     VQHRLPIIIAILNNGFLGMVRQWQQLFYNKRYSYTCINFQPDFVKLAQAYGAEGIRIE
                     KKADVVPAIQKAFTITDKPIILDFHVDREENVYPMIPAGKSVKDMMMRELA"
     gene            9090..9566
                     /gene="ilvN"
                     /locus_tag="PLJ75_10040"
     CDS             9090..9566
                     /gene="ilvN"
                     /locus_tag="PLJ75_10040"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPD05592.1"
                     /translation="MSQHVISVKVENKFGVLARVAGLFSARGYNIDSLAVSETELPDV
                     SVMTIVVSGDDRIIEQVKKQLNKLIDIIKVTDHNQENAVQREIVLAKLNATPTKRNEI
                     YQLAQIFGARIVGVTNKAIILEMVSDPETVDNFIELLRPYSIKELIRTGRVSILKE"
     gene            complement(9632..>9902)
                     /locus_tag="PLJ75_10045"
     CDS             complement(9632..>9902)
                     /locus_tag="PLJ75_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05593.1"
                     /translation="MQKGMQKGMQKGMQKGMQLGMQKGVVRGKQTGLKEGIVQGMIKE
                     RAKIASKMLRNGIDDATILKITKLDKNQLEILKNKNKNENKKKKK"
BASE COUNT         2916 a         1906 c         1558 g         3522 t
ORIGIN      
        1 ccaatcaacg tatttgccgc tacaaattta aactcaagat ttggcaatgg tattattccc
       61 ctgttttgtt tttcatcatc aatcttttca tctacaataa gggaaaggaa aaaccgtagt
      121 tttgatattt caactgcaat gggctgtata tcaacgccat atatggaatg ttgtattaac
      181 cctaatttgt gaatgtaatc ccaattttct tcctccaatt ttatttgcaa ttccttctgc
      241 aaaagcgggt caacaatttg cccaatcttt tttttaaacc atatttttga ctgtggatca
      301 acattctcca gaattacctg caatttgtgc agtataccca tagggaaggc accgcttccg
      361 catgccggat ctattacttt aattttgtct atggcatcta tgatcgcttc tttatcatgt
      421 tcattaaggt tagcagcagc ttcaaaattt tcatgtttca ataattcttc aatgatatct
      481 ctggatagtt ttgttttctc acacaaatag tttttaagcg attcatcaac catataatca
      541 actatttcgc gcggggtgta ataacttcct gtagctttgc gcgcattctc ttttgtttca
      601 gggttatatt ctgccaaaag gttttcaaat attttaccca acatttcagg atcaatggac
      661 acttcagtat caacaagtgt attctcatca atggtaaaat tatatcgctc aaaaacctca
      721 aaaaggtttg caaaccagtc atttgatatt gtcagtatat ttatatttgg tgatattccc
      781 atttcgttta ttgcagttac aggataataa tcactgtcgt gtggttcaaa taaacctcca
      841 ttcaaaaatg gaataagcga ataactttta gttttgtatt tttctaacct ttcttttaat
      901 ggtttattta atatctgaaa gaataatggc tcaacatatt catggtaata gtctccatta
      961 aaatttttta ccaactcaga tgaaagatat ttctggggta tcaatgaatc tttagaacca
     1021 tccttcttca tcttcaaaaa ccaacaaaat aaaagtctac ccagtaaccg aatggcaaac
     1081 tcttcctttt cattttgatc ttcctttgga aatcgttgta atcccttgtt attgtagttg
     1141 ttaataagtg tataatataa ttctactata tttctataaa actctttcgt aaccttttct
     1201 acactaaacg cgtcaatgat tttttctaaa ctttcaaagc ttgctgcttg catttgctgc
     1261 aaaaatgtcc ggtttgtctg gtctttactt acatagtagg tgtaacgcct gaagttactc
     1321 cagtcctgct tgccatcggg acggggtatg ctatagataa acgaaaacct gaaatttcca
     1381 ttgctgtcgt aaaaaataaa aaagccacca gtgtattgta tcatttgttt caatatcttt
     1441 tttgcaaagg tgtattgcgc tttttttccg cttttttgtg ttaattcttt atttactttt
     1501 gcggcaatca caataaattc gtgaacatcg ttaactttaa acgtgcctat ttgcgtgctt
     1561 tcatcaaaaa gttcgtcgtt acatccatct aatggcttat cttgagatat aaaatcattt
     1621 gtttttttat aaaaaaaatt tttaaattta tttacgtcaa aatcatttat aatatcttca
     1681 agtattctaa ccacatcatc catgctgcta ctccatttgt tcctttaatt ttatatttta
     1741 ttttctattg caataattat ttccttttgt aacccatctt ctttagcttt tattttgtgt
     1801 aagtaatcat tgcctaattc ctgctgcaat gcagcaattt cgttaaccag ctcctgtaac
     1861 tttgtagcat cgttgtagtc aatgttgaaa tttgcaatcc gccgcaatgt ataatccgaa
     1921 agggttccat aatcaataag atcttcttta agctgtttta aaaatggaat atacggttgc
     1981 agtgcctccg ggcactcgtt gcttaagata aactgtagct tattctttgc tctatcctgt
     2041 atactcaaca cactttttgg taagctggat ttatctttat gcacttttac tttttcatat
     2101 gcgccccaga aacgttcgtc tattggcaaa gccttttcat caatattgca tgcaattcta
     2161 tccactactt cttcaagggt agtttctaca atactgccat ccgaataatc atagttcata
     2221 acaaacaatc tgtttttctt aaagaatacc agcaattcat catacgtgct tttctttgat
     2281 actttcactc tgtgtggagt gctttctatg ttttttataa tttctggata ctgggccttt
     2341 atactttcaa atacattcaa aactctggta tagaagcttt ccgcttctaa tgtttctgga
     2401 tttatctgta aacggtgata gagttcggct ggtgttggtt cttcgtcctc gtcaaaaatc
     2461 tttgcgtcct cacccaatgt attgtgtatc atgaacattt tattctgggc tatctcgcgc
     2521 gatttaacta tctcagcacc cttttcggtt gggaaaaagt ttactatata cagctcatca
     2581 aacacttttt tgcttatacg gtttatacgc cccaatcgtt gaataacacg cactggattc
     2641 cacggtatat catagtttac aatcatacct gctctgttta agttaaatcc ttcggaaagc
     2701 ttatcggtac acagcaatat gtcataatca tctttctgat cctgatacga agcatcaaag
     2761 tttttataaa tttcttttat tttactatca gacaaatcac cggaaataac cagaacgcga
     2821 ttgttaaatt tattatttaa tgtatctttt aaataacaaa cagtatcaag aaactcagaa
     2881 aaaatcacaa tttttcgctt gtttttatct ttttctttat tttgataaaa ttcatttgct
     2941 acatttttta ttaggcaatc cagctttgga tcattgttta ccagatcaag cgcatcaagc
     3001 tgttccagta tggaggtaaa taactttatg tcatgtttta tatcatcaat aaatttttct
     3061 ttatatttaa aggtcttaat tttatatcgt ttatattttt ttggtaaaac atccttttgc
     3121 aggttcgttt catactcctg taaatattgt tctatttcat caacgtccat atcgtaaatc
     3181 ttttcaagca attgcctatc caaaataaat tcgcctttaa gtggatctcc acctccggtt
     3241 ttttcaataa actgtaatac atgttcatgg atacgtttaa aattttttat actctcttta
     3301 aacgcaccaa acgagctttc aaaccgttta acaagaagcc tgcgcataaa attaaaaagg
     3361 tttttctgtt gttgatattc acggtttttt tctaaaagcc cttcgtttac atcattatca
     3421 tcatttattt caagctcacc tacttcgtat ataaatggcc ggtatataga tcctttgaaa
     3481 gcctctacgt catcctcaac aaaataatgt tcaatgatag tatcataaaa ttgtagctgc
     3541 ttaggtgtta attcataaaa ccattctatc ggatccttta caatagataa atgttgaact
     3601 tcatccttat agtacggatt cttttgtaaa tcaatgcgat tacgtctaat cgtaactggt
     3661 tcaatgacat cgcgtatctg ctttgccagc ttatgcgttt cagcatttac cctgcgtata
     3721 tctatgtttt ctgttccaaa tagatatttg taaaacgttg tagctctatt tcttttttcc
     3781 cgatctttcg aatcatgata ccgttttata taacttagct tatcaaattt agatttatac
     3841 atggaaaaca aaagctcaag gttgttttca agggtaatgg ttgattttct aggagtcaca
     3901 aacagtttta tcaatgaaaa tatatccgaa ggtcggttat taaaaggggt agctgttaat
     3961 aaaattacta ttttatctct gcatatgttt ttcagcagct catacgaacg ggtgtcctga
     4021 tttctaaatc tgtgcgcctc atctacaatg acaacttcaa tatccttgca tctattcaca
     4081 aattcaagtg cttcttctaa tttcccgctg gaacgcactt cccaatcaaa aagcctgaaa
     4141 tcttctaaat attttttcca gcccatatcc ttattgtcat taccaattaa cccgggtggg
     4201 catattacaa tgcctcgttt ttttaactga aacgcaatgg cacaggcaat gatagttttc
     4261 ccaagaccaa caacatcggc caatataacg ccattgtgtt tttctataat agctatcgcc
     4321 tgtgttattg catctaactg atagcggtat ggcacgtagt tgttgcttat cagtatcctg
     4381 tttatcgctt cactggcagt ccgtatttca tacgtatcta gatacgattt taatacaagg
     4441 cagtatgctt caaacggtgt tatttcttta ataagtgtct ggtgttgtaa caaatttata
     4501 agctcttttt tgcaggcatc attttcagta atagcaaccg attcatccca tattgtatca
     4561 aaataccttt cggcttcttc gataccatag tcagttattt ctacattaaa ttcatgctgg
     4621 ccaataagcc ctgcccgggt taggttgctg ctaccggtta ttagtatttt ttgttttacc
     4681 tgttcagttg aaagtgtaaa aaggtacaat tttgcatgat tgggctggcg ggtctttcgt
     4741 attattaatc gttcattttt aattaattga ataaaatatg ctatctgctc ataaaaatct
     4801 ttattgtcaa attcttcgct atttattgac tttgtaattg attcaaaata tctattaata
     4861 acatctttgt tgcttacatt agcatcatta ccacattcaa caatacaata atttagagta
     4921 tcaacgttaa ggccaacaca aatctttaca actacatcgg cattgttttg caatgaggta
     4981 tacaaatctt taatccccga aaaatagaaa aaaccaacta aaaattttaa ttctttgcta
     5041 tgtgcaatca aatctcgaac tctatgcagt aaatttttag ttggttgatt ggtaataaag
     5101 ttggacataa taaaccttta caatgtaata tgatttttcc gtacttacta ttaatccatg
     5161 taatatcctt ttaacataca acaactatgg gttaatctgt atatacaatc taactttcct
     5221 ctgtcaataa ctttgtaata caaatgagct atttgtcgtc ataccctata tattccttta
     5281 ctcttctatc atattcgttt attttaacag taacagcatg cctggtgttt gtgccaatat
     5341 actgttcggc agctacaacc atatagggaa atacatatcg taagtcgttg ctactgccaa
     5401 tgcttactat atctgttttc cataactgct tttccacctt tttagtttta aacaatttcc
     5461 agtcaaaagc aattaatata atagttctgc tatagaatgt tcgtttcacc tgtgccactc
     5521 cagttattcc gtatgtttga ttataatatg tgtttccata ataactgccc gatgaggata
     5581 tattgccata ggttgttgaa gattctatcc ctgttggtcc ccatagtgga acatttgcag
     5641 tataattttg tggaccgctt atagcataca caagcatcac aacctgatct gcctcatcga
     5701 tagtattgac tattgcaaac ccttttttta ttaatacctt tttaatatag ttgctaaact
     5761 cctgaaactg caaatcatta atagcaacat ccttcattcc gggtgcaaca aacactttat
     5821 tgcctgcagt ctcattatta ttgatagacg ataccataac ctcataatga gcacacccta
     5881 ttgcaaagaa caacagcata ataaccatac ttttagcttt catagtataa acctccaaaa
     5941 tagtttgtat ataaattaac taccgggcag ggacatatac tgtccctgtt aaaaatattt
     6001 ttactttgat tttacaaacc gcacgtatgc ttcttttatt gcttcaagcc acatggtgcg
     6061 tatcgatgaa ggtgcaacaa tctcggcata tttgccatac cgcaaaacct tccctaaaag
     6121 ctcctgagga tgatgaaccg gaagctgcag ttcaacgtag gcaccgtact gttcatgttt
     6181 tccttctttc atggtctgct ctttatgcca tacctgtttt tttactatat tacataccgg
     6241 ttggtaaaaa cgaacctgca ctaatttgcc accactgcct ttataaatgc catatgttga
     6301 cttaataaat ttttgtaatt catcatcatt aatttttctt gtacgctttt tatttacaac
     6361 ctctgctttc tgtatgcgcg acagtaaaaa tgttcgtatc ccttttttag tttcatcata
     6421 ggcaatgaca taccaccttc cctcataatg aataaagtgt tcgggggcga tagttctggt
     6481 agtagtcttt cctgtgacat cagcatattt aattacaata caattatcat tccgaagcgt
     6541 tgaaaaaagt ttgctgaaca gagtaatatc caactgttcc cattcgtcta tttcgtaggt
     6601 aatcctggac ataaggggga gatagtgagc aagaatattg cgctttatca ggtcggggat
     6661 agctttatcc acaaagggga aatgtgtggg actttcaatt aatttcatta agaataaata
     6721 aaataacagc atgcgttcat tgcggtagga aaaataatca aaaggcaaag aatacaaata
     6781 tcctttttta tatacatcat atactattgg tgcgttaatc cggtctctga aatattcaag
     6841 atcgcgctta accgtccgtg gcgacacttc aaatttctcc acaacctctt taactgtcac
     6901 aactcccttt tcgcgaatgc tggtatcaat atactgcaac cgttcattct gactcaaggc
     6961 aaacctcgtg ctaatttttc ttcaataatt ttttatttta attattataa catattatgg
     7021 tatgttgtca attatcattt atatacatgt ttgtgtaatg ctatacgggt tacagctttt
     7081 actcacggta gtattgctct tatctgcaaa tagcattatg cactactgtc attttaggct
     7141 ttatcttgaa tctctttatt accatgtgat cctgaaaatt acattgccgc tacaatgtat
     7201 tatatataaa gaaaaccaat tatcagtgac tatcgcccac tgctattttt tataagtgta
     7261 tgctatcaat gcttataata gtttaaatgt ttatattata ttttatatat tattttatat
     7321 ttttataatt taaaaaaaat tatgttgcaa tttttatgcc tgctatattt agatatatgc
     7381 ttacttttta tttatttctg cgaggtgtaa caggtgaaaa ttacaggtgc acagattatt
     7441 attgaatcac ttatcaggga aaaggttgaa gtcatgtttg gctaccctgg cggcgtggtt
     7501 atacctattt tccaccattt atttaatgca ccttttaaat ttattttatc ccgtcatgag
     7561 cagggggcga tccatgctgc tgatggttat gcccgtgcaa ccggtaagcc cggtgttgtc
     7621 attgcaacct ccggtccagg tgccaccaat ttagttaccg gcattgcaac agcaaatatg
     7681 gattcgatac cgcttgttat attcaccgga caggtatcca ctgaggttat tggcaatgat
     7741 gcatttcagg aagctgatgt tacagggata acccgcccaa ttaccaaaca taattattta
     7801 gttaaggata ttaatgagtt agccaaaatt attaaagaag ctttttatat tgcaacaacc
     7861 ggcaggcccg gccccgtatt aattgatgtt cccgtcaata tatcaaatgc cgaaaccgag
     7921 ttccattatc cagaaacagt gcatttgcga agctacaagc ctgtcattca tggtcattca
     7981 gggcagattc agaaagcatg taaattaatt gaacaatcaa aacgtccagt tgtgtatgct
     8041 ggtggtggtg ttatatcatc caatgcatcg gatgaattgc gtacattcat aaaaaagaca
     8101 ggtattccca ttaccactac cctgcttggg ttaggtgcct atccggaaaa tgacaaactt
     8161 tcgctcaaga tgttaggaat gcacggcacc tattatgcaa accatgctgt tcatcagagc
     8221 gacctgctta tagctgtggg tgcacggttt gatgatcgcg tgactggtaa aatttcagag
     8281 tttatacctc atgcaaaggt catccatatt gatatcgatc cgtcatcagt tagtaaaaat
     8341 gtagctgtcg atgtaccaat tgttggtgat gcgaaacatg ttttaaccga gataaacaaa
     8401 acagtaaagg ctcccaaaat agatgagtgg gttactcatg taatgaagat gaaagaagaa
     8461 aacccgctta cctataaaga cagtgatgta accattaaac cacaatatgt tattgaaaaa
     8521 atctatgaga tcagcaacgg tgatgctatt atatgcactg aagttggaca aaatcagatg
     8581 tgggcatcac aattttttga ctatacacag ccgcgaacct ttatatcatc aggcggactg
     8641 ggcacgatgg gatttggatt tcctgcatct attggagccc aattaggccg tcccgataaa
     8701 cgtgtaattg acattgctgg tgatggttca atacagatga atatacagga actcattgta
     8761 gcagtacaac atcgattacc tattataatt gctatactca ataacggctt tttaggtatg
     8821 gtacgacagt ggcaacaatt gttttataat aaacgatatt catacacctg tatcaatttc
     8881 cagcccgatt ttgtaaaatt agctcaagcc tatggtgctg aaggcattcg tattgagaaa
     8941 aaagcagatg ttgtacccgc aatacaaaaa gcgtttacca tcactgacaa gccaataatt
     9001 ctggatttcc atgttgatcg cgaagaaaac gtttatccaa tgatccccgc agggaaatct
     9061 gtaaaagata tgatgatgag ggaattagca tgagccagca tgtaatatca gtaaaagttg
     9121 aaaataaatt tggtgtgtta gcacgtgttg ctggactttt ttctgcacga ggatacaaca
     9181 tcgactcgtt agcagttagc gaaactgaat tacctgatgt ttcggttatg actatagtcg
     9241 ttagcggtga tgaccgtatt attgaacagg taaagaagca gcttaataaa ctcattgata
     9301 ttataaaagt aactgaccac aatcaggaaa atgctgtaca gcgagagatt gtattagcaa
     9361 aacttaatgc cacacctaca aaacgtaatg aaatttatca actggcacag atttttggag
     9421 cacgcattgt tggtgtcact aataaagcaa ttatactgga aatggtcagt gatcctgaaa
     9481 cagtagataa ctttattgaa ctgctgcgac catattctat taaagaactc atccggacag
     9541 gcagggtatc aattcttaag gaatgaaatc gtaaaatttt ttattatatt tgctgcatca
     9601 ttcatttaat aatattagtg atatcaaccc attacttttt ctttttctta ttctcattct
     9661 tattcttatt cttaagaatt tccagctgat ttttatccaa ttttgttatt tttagtattg
     9721 tcgcgtcgtc tatcccattc ctgagcatct tactggcaat ttttgcacgt tcctttatca
     9781 taccctgcac tattccttct ttaagaccgg tttgtttacc tctgacaact cctttttgca
     9841 ttcccaattg catacctttt tgcatacctt tttgcatacc tttttgcata cctttttgca
     9901 ta
//
LOCUS       DAOOJH010000087         9851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6137_length_9851_cov_9.754185, whole genome shotgun sequence.
ACCESSION   DAOOJH010000087 DAOOJH010000000
VERSION     DAOOJH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6137_length_9851_cov_9.754185"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLJ75_10050"
     CDS             complement(<1..433)
                     /locus_tag="PLJ75_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HPD05594.1"
                     /translation="MKMLNKITVWASMFLLPGFIAAIALSMGPTDVTIFSVINNRSAT
                     TAAILLLRLQRVSMAFIVGAALATSGCIFQALLKNPLAEPYTLGVSSGAALGATVAII
                     FTMNWISAFAFCGGLFTVIVIYVIAKKIQFNTTAIILAGISM"
     gene            complement(532..1362)
                     /locus_tag="PLJ75_10055"
     CDS             complement(532..1362)
                     /locus_tag="PLJ75_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HPD05595.1"
                     /translation="MKPLHSIMPCILFLSIIFVILFLTVHAITPHQLSHRQDVQSQRI
                     IALSPSITAMIIDLGCEHLLVGVTTFHPPLSKNLPIVGTMTHPNIEKIIELAPDTIFI
                     SRDDAVTQKIDQLNYVKNAALYILPVSTTFDDICAHYMTVAKILNKEDYAVKKLHYYK
                     KQRQAHHHPESTKAIILISVNPLIAVSHHSYISNIFGDMGINNVITYSKSAYPIIQKE
                     YLLSSDADIIIELCNDHLQQTITKIIPVLRIPYEPVYFYTPRHYCQSLQYIATFLCSQ
                     "
     gene            complement(1720..2958)
                     /locus_tag="PLJ75_10060"
     CDS             complement(1720..2958)
                     /locus_tag="PLJ75_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPD05596.1"
                     /translation="MKQKSAPHNYAVFVARQPIFNRHKELYGYEILFRNGFENFYNSL
                     DPDASTSRTLINSFLVIGTDSLTGGKRAFINFTEATLKTSIASFFPKEVLTIEILETV
                     SITPEIITITKKLKSMGYKIALDDFVLSDEYRELLPYADIIKIDFLNTTTFERLQILN
                     EVSRFNTRLLAEKIETYEDFHEAISMGYSYFQGFFFSKPEIIESKDIPIYKLNYLEIL
                     QQIHKEDVDFGKLEDIIKRDVSLSFKLLKFINSAAFGFASEIASIKHALALLGIREFK
                     NWLSFIVLHSIAQDKPQELIVTSLIRAKFAELLASSSQFSTHTSECFLMGLFSLVDAF
                     FDKSKKEILMQLPLTGDIKNAIMDMESPYSGIYKFITAYEKGDWEIVNKLSKQLQFPP
                     EQITQSYRKAIEWANAFFMH"
     gene            complement(3276..4433)
                     /locus_tag="PLJ75_10065"
     CDS             complement(3276..4433)
                     /locus_tag="PLJ75_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD05597.1"
                     /translation="MYDFGLTEEQKLLRQNIRDFAEREIFPVAQELDETETFSVELTR
                     KMGAMGLFGIVVSQNYGGAGMDYLSYVIAVEEIARIDGSQAATVAAANSLGIGPIYYF
                     GSEKQKHEWLPKLCSGKILASFGLTEPGAGSDASASKTNARLENGEWVINGSKIFITN
                     STSSMAGVCTVQAITGKRTDGKKELSCILVPNGTSGFTAVKMTRKMMWRASDTGELFF
                     DDCRVPEENLLGQRGEGFHQMLSTLDNGRLSIAAMGLGGAQGAYELALKYAKQRKQFG
                     QPISTFQAIAFKLAKMATEVEAARNLLYKACRLKDAGMPFSKHAAMAKLYCSEVMGHV
                     VDEAVQIHGGYGLMKEYNVERFYRDYKLLTIGEGTSEIQKIVISRQIGCYE"
     gene            complement(4489..5079)
                     /locus_tag="PLJ75_10070"
     CDS             complement(4489..5079)
                     /locus_tag="PLJ75_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05598.1"
                     /translation="MSMFGQVYVKNNQYKLKGDFHELTPNIPIRNADEGWRLMGVTNP
                     RDMTYIHAYGGEAIFFESLPKGKLVSSRCDNPKCKYKGSIWMPFRIYCPDCLRPMAVF
                     DMTDLAKKTARIHTFMVCERSGAFNILDKPIKFINIEFDGVVTILMSYLLVGEPKIGM
                     RVVPIFKKVDPTYTILDLSWVPEGTKEEQLPIGFSF"
     gene            complement(5092..6528)
                     /locus_tag="PLJ75_10075"
     CDS             complement(5092..6528)
                     /locus_tag="PLJ75_10075"
                     /inference="COORDINATES: protein motif:HMM:NF004982.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HPD05599.1"
                     /translation="MITFTQQQSKIPKLMRPVYIVTAGVSKFDRAFPDKRTEELCIDA
                     FTMASDMLDMEPAELKGYIHTCYYGHFADHFGDQLLGEAVIHDRLGLDPLGNIGVKTG
                     GATGGSTLWEAVKAVASGYSDCTLAMGWERMDEVPTDEGNFLISCAADKDWESPLGHI
                     YTGYYAVMAQKYWQVFGKEEDSFRRTMAEISVKHHNYARFNPYAQGPMKITIDDVLKS
                     PVVAYPLRALDACLMSVGAACAIICDEETAIKLTQKSKNKPLRIWVSAGSHTLRPADR
                     RDMEIPLLPNETKEQYKDFGKRFPGGERYPGFTGFLAARMAAWYGYRMTGITNPMEDL
                     DVVELHDAFTISDIQTYEDIGLRPYGYGRDYVESGDCYHINPKTGKPGRLPSNLSGGL
                     IGCMHAVGATGIMQVFEIACHLWNRWAEMHGDEKYWKRCKRTKPDDWTNLQVKDAKRA
                     MAISHAGVGSHVTATILMDADSLLKKDA"
     gene            complement(6632..7174)
                     /locus_tag="PLJ75_10080"
     CDS             complement(6632..7174)
                     /locus_tag="PLJ75_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPD05600.1"
                     /translation="MDLGSVLKIKRKEKKMTLKTVASRAGISEGFLSQIENNVNSPSV
                     DTLINICSSIGCDAGEVIQQAQNQKKISIIRKKDLTEIDIPHVGFATLRFFSPESRRT
                     IDSAILMVEPGKSIPARKGIRNSQEVLCVLKGSLELTYQNQVFVLYQGDAAHFITQPE
                     QQHITNNSKMIAVALWVGTI"
     gene            complement(7452..>8032)
                     /locus_tag="PLJ75_10085"
     CDS             complement(7452..>8032)
                     /locus_tag="PLJ75_10085"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPD05601.1"
                     /translation="TLIIKGKIASIMPHRAGTIITIRFDAYSHNNQPVFTEHIGGMLR
                     GVECTGTVTENSNIPIPGFSEDTSSPCWEKKVYIDRLRPYVYDGCSNIHFPIHTSVAF
                     AKMVGLPDIILQGTATLAYTAREIINHEADGNPHVLKVLSCRFTGMMIPPGNINIALY
                     SKKQVNQNRHLFFEVHDDTGQLLARGNAHLSY"
     assembly_gap    8033..8132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8133..8482)
                     /locus_tag="PLJ75_10090"
     CDS             complement(<8133..8482)
                     /locus_tag="PLJ75_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPD05602.1"
                     /translation="MKLDASFVGTTLKDYTTTISSRAMMNYAAAIDDNNPYYFNDEST
                     EGIIAHPMFAVACTWPISEKICDYINNKNFPLEIIKTQVHYTEHLEFHRPIAPNDTLI
                     IKGKIASIMPHRAGT"
     gene            complement(8604..9641)
                     /locus_tag="PLJ75_10095"
     CDS             complement(8604..9641)
                     /locus_tag="PLJ75_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015468990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05603.1"
                     /translation="MGTNPDRDNVSMFKHSMHYDANKNMFVNRNQDAIDEMWDKNITF
                     TIMIDMFKGRKILKPTTKLPELKPDLSVFLQQDSDLRVIWFGHSSFMLNIDGIIVLVD
                     PVFTNSSFPVNIMNKRFQPPVLKLNELPVIDYILISHDHYDHLDMKSVKYFIDKKARF
                     VVPLGVGSHLKSWGIPHDRIVERDWWVTAKFDSIQFIATPAQHFSGRGLFDRNKTLWA
                     SWVIKSKNHSVYFSGDSGYDIHFKAVGKKYGPFDIAFLECGQYNNQWREVHMFPEDSV
                     TAFKDLRAKKFFPIHWGMFILSKHPWNDPPKQLYALSKTHKIDLIIPKIGQLVNVNHT
                     FQLEEWWNGDK"
BASE COUNT         2922 a         1884 c         1765 g         3180 t
ORIGIN      
        1 acattgaaat tccggcaagg atgatagcgg tagtgttaaa ctggattttt tttgcaatga
       61 catagatgac aataacagta aaaagcccgc cacaaaatgc aaaagcagaa atccaattca
      121 tagtaaagat gattgcaacc gttgcgccca atgcagcgcc gcttgaaaca ccaagcgtgt
      181 aaggctcagc taacggattt ttaagcaatg cctggaatat acatcctgat gtggctaatg
      241 ccgcgccaac aatgaatgcc atgctcacac gctgaagccg cagcaacagt attgcagcag
      301 ttgtagccga cctgttgtta atgacggaaa aaatggtaac atcggtggga cccatagaga
      361 gagctatcgc cgcaataaaa ccaggcagta aaaacattga tgcccatact gttattttat
      421 tcagcatttt catattacca tacagtatat catgtagcgg tatagccgtt gtcaagtttt
      481 taaatgtcgg gcgatagttc ctggcataca attaattttg aagataggca atcactgcga
      541 gcataaaaat gttgcaatat actgcaatga ttgacagtag tgccggggag tataaaagta
      601 tactggctca tagggtatgc gcagaacagg aataattttg gtgattgttt gttgcagatg
      661 gtcattgcaa agttcaatga taatgtcagc atcagacgat agaagatact ccttttgtat
      721 aatgggataa gcactttttg aataggtaat tacattgtta atacccatat caccaaatat
      781 attgctgata tacgaatggt gcgaaaccgc aatgagtgga ttaaccgaaa tgaggataat
      841 tgctttcgtg ctttctggat gatgatgtgc ctgcctttgc tttttatagt aatgcaattt
      901 ttttacagca tagtcttctt tattaagaat ttttgcaacg gtcatataat gagcgcatat
      961 atcatcaaat gttgtcgaaa caggcagtat atacagtgca gcatttttta catagttaag
     1021 ctggtcaatt ttctgagtaa cagcatcatc ccgggaaata aaaatagtat cgggagcaag
     1081 ttcaataatt ttttcaatat tgggatgggt catagtgccc acaatgggta aattttttga
     1141 aagtggtgga tgaaatgtgg ttacgcctac cagcagatgt tcacatccca ggtcaataat
     1201 cattgctgtg attgatggcg acagggcaat gatgcgctgg ctctggacat cctgtctgtg
     1261 tgatagctga tgtggtgtaa tagcatgcac tgttaaaaat aaaattacaa aaattatgga
     1321 aagaaacagt atacatggca ttatgctgtg taacggtttc aaactattga taatcgttcc
     1381 tccttagaaa ataaaattat tgttcctttt gggcgcatcg taatgagctt gcttaaccat
     1441 accgtcatac cgagcatgac acggaatatt atttgctgtc tttgtcattc cggacctgtt
     1501 ccggaatcaa gatgtgcaac gttacatcct gaaaccagtt caggatatga ttcaggatga
     1561 ctgtcttccc tcgtcatacc ggacgtaaca cggaatgcaa ataattaaat gagatccttc
     1621 actttgactt cactcatcat aggcgctatt gctttacaat gacaggatgc ataattattc
     1681 attacaagaa aaaaagaatt ataacagtat agcaatgtat taatgcatga aaaatgcgtt
     1741 agcccattct atggctttgc gatatgattg cgtaatctgc tcaggaggga attggagctg
     1801 tttggaaagt ttatttacta tttcccaatc gcctttttca tatgcagtga tgaatttata
     1861 aatacctgaa taagggcttt ccatatccat gattgcattt tttatatcgc cggttaacgg
     1921 caattgcatt aatatttctt ttttgctttt gtcaaaaaac gcatcaacaa gcgaaaacaa
     1981 ccccattaaa aaacattcag atgtatgtgt ggaaaattgc gatgatgaag ctaatagttc
     2041 agcaaatttt gcacgtatta acgacgtaac aataagttcc tgtggtttat cctgcgctat
     2101 gctatggagt acaataaatg aaagccagtt tttaaattca cggatgccca gtaatgccag
     2161 tgcatgttta atagaagcta tctcacttgc aaatccaaaa gctgccgaat tgataaattt
     2221 taaaagttta aagcttagcg atacatctcg tttgatgata tcttccaatt taccaaaatc
     2281 aacatcctct ttgtgaattt gttgtaaaat ttccagatag ttgagcttat aaatgggaat
     2341 atcttttgat tcaattatct caggtttact gaagaaaaat ccctgaaaat atgaatatcc
     2401 catggatatc gcttcgtgaa aatcttcata cgtttcaatt ttttcggcta aaagcctggt
     2461 attaaaacgc gaaacctcat taagtatttg taagcgttca aatgttgttg tgtttaaaaa
     2521 atcaattttt attatatcgg cgtatggcag taattctctg tattcatcag aaagtacaaa
     2581 atcatccagt gcgattttat aacccatgct cttcaatttt tttgtaatgg taatgatctc
     2641 cggtgttatg gagactgttt caaggatttc tatggttaaa acttcctttg ggaaaaaaga
     2701 tgctatgctg gtttttaacg ttgcttcggt aaaatttata aaagctcttt taccgcctgt
     2761 caatgaatcg gtaccaataa ccagaaaact attgatgagt gttctggatg ttgatgcgtc
     2821 tggatcaagt gaattgtaaa aattttcaaa accattacga aataatattt catatccata
     2881 taactctttg tgcctgttaa aaataggctg gcgagctaca aagacagcat aattatgtgg
     2941 tgctgatttt tgtttcataa taaaaattta attactcatt attatgcaaa aatatcatat
     3001 attcatataa ttggcaacaa ttttattata gtaactattg ggtggctaac tatatatttt
     3061 gtcaataagc aaaaatggtc attgcgccat ttccttcttt aaaaagtata tagtattgat
     3121 aaccgtgatt ttgctgcatc atgagatgaa aacactatgg gcatctttcc catatttata
     3181 aagtgtaaaa gggtatattg tagacattta ctgtataaca aaactatgcc ttgttaccat
     3241 gattttttaa ggtaaattcc ttgaaaaatg attatttact cataacaccc tatctgacgt
     3301 gatataacaa tcttttgaat ttctgaggtt ccttcaccta ttgtcaatag cttgtaatcc
     3361 ctgtaaaagc gttcaacatt atattctttc ataagcccat atccgccatg gatttgcact
     3421 gcttcatcaa ctacatgtcc catgacttcc gaacaatata attttgccat tgcagcatgt
     3481 tttgaaaaag gcatacctgc atcttttaaa cggcaggctt tatagagcaa atttcgcgct
     3541 gcctcaactt ctgttgccat ttttgctaat ttaaaggcta ttgcctgaaa tgtagaaatt
     3601 ggctgcccaa attgttttcg ctgttttgca tatttcagcg ccagttcata tgcaccctgt
     3661 gcacctccta aacccattgc tgcaatggaa agcctgccat tatccagggt agaaagcatt
     3721 tgatggaatc cttcaccgcg ttgccctaac aaattttctt ctggtacccg acagtcgtca
     3781 aaaaagagtt ctccagtatc ggaagcacgc cacatcattt tacgcgtcat ttttactgca
     3841 gtaaatccgg atgtgccatt tggcacaagg atacacgata gttctttttt gccatcggta
     3901 cgtttaccgg ttatagcctg taccgtgcac acacctgcca tagaagatgt ggaattggtg
     3961 ataaagattt tacttccatt aatgacccac tcgccatttt ctaaacgggc atttgtcttg
     4021 ctggcacttg catcagatcc agcccctggc tcggtaagtc caaatgaagc aagtatcttt
     4081 ccggaacata attttggcag ccattcatgc ttttgttttt cactgccaaa ataataaatt
     4141 ggtccaattc ctaaagaatt tgctgctgcc actgttgctg cctgtgaacc atcaatacgt
     4201 gctatttctt caaccgctat aacataggat agataatcca ttccggcacc accataattt
     4261 tgcgaaacta caataccaaa aagccccatg gcgcccatct tcctggttaa ttctacagaa
     4321 aaggtttcgg tttcatccag ctcctgtgct acaggaaaaa tctccctttc ggcaaaatct
     4381 ctgatattct ggcggagaag tttttgctcc tctgttaagc caaaatcata catggcaaac
     4441 ctctttttta aaaatcttta ttttgtttta tcaaaaaata tatgaatgct aaaatgaaaa
     4501 tccaattggt aattgctcct cctttgtgcc ttcaggcacc catgaaagat caagtatagt
     4561 gtatgttgga tcaacttttt taaaaattgg cactacccgc atcccaattt ttggttcacc
     4621 aaccaacaga tagctcatca atatagtaac gacaccatca aattcaatat ttataaattt
     4681 tatgggttta tcaagaatgt taaatgcgcc agaacgttcg cataccataa atgtatggat
     4741 acgagctgtt ttttttgcta aatcggtcat atcaaaaact gccattgggc gcaggcaatc
     4801 aggacaatag attctaaatg gcatccatat gcttccttta tatttgcatt ttggattatc
     4861 acatctggat gataccagtt tgccttttgg aagtgattca aaaaatatcg cttcgccacc
     4921 ataggcgtgt atatatgtca tgtcacgggg attggttacc cccattaacc tccagccttc
     4981 atcagcattg cgaattggta tattaggtgt taattcatga aagtcaccct tcaatttata
     5041 ttggttattt tttacgtaga cctgtccaaa catactcatt gtattacctc cttatgcatc
     5101 cttttttaat aagctatcag catccatcaa aattgttgct gtcacatgcg aacctactcc
     5161 ggcatgactt attgccattg ctcgttttgc atcctttacc tgtaagttgg tccaatcatc
     5221 aggtttggtg cgtttgcaac gtttccagta cttttcatcg ccatgcatct cggcccatct
     5281 gttccataaa tggcatgcaa tttcaaatac ctgcatgatt ccggttgcac caacggcatg
     5341 catgcaccct ataagccctc ctgataaatt tgatggcagc cgacctggtt tgccagtctt
     5401 tggattaata tgatagcaat cccccgattc aacataatct cttccataac catatggtcg
     5461 taaccctatg tcttcatacg tctgaatatc gcttatggta aaagcgtcat gcaattctac
     5521 aacatcaagg tcttccattg gattagtaat gccggtcata cgatagccat accatgctgc
     5581 catccgtgct gctaaaaatc ctgtgaagcc agggtatcgt tcgccgccgg ggaaacgttt
     5641 gccaaaatcc ttatattgtt ctttcgtttc atttggcaac agcggtattt ccatgtctcg
     5701 ccggtcagca ggtcgtaagg tatgcgaacc ggctgatacc catattcgca atggtttgtt
     5761 ttttgatttt tgtgtaagtt ttattgctgt ttcttcatca catataattg cacaggctgc
     5821 tccaacactc attaaacatg catcaagtgc ccgcagtgga taggcaacca ccggtgactt
     5881 taatacatca tcaatcgtta tcttcattgg tccctgtgca tagggattaa atcgtgcata
     5941 attgtgatgt tttactgaaa tttctgccat tgttcttctg aaagaatctt cctctttgcc
     6001 aaatacctgc cagtattttt gtgccattac cgcatagtat cctgtatata tatgtcctaa
     6061 aggtgattcc cagtctttat ctgctgcaca ggagattaaa aaattgcctt catcggtagg
     6121 aacctcgtcc atacgttccc accccattgc cagtgtgcag tcagaataac ctgaagcaac
     6181 agctttaaca gcttcccaca aagtggagcc accggttgcc ccaccggttt ttacaccaat
     6241 attacccagt ggatcaagcc ctaagcggtc atggataacc gcttcgccta acagttgatc
     6301 gccaaaatgg tcagcaaaat gtccgtaata gcaggtgtga atataccctt taagctctgc
     6361 aggctccata tccagcatat ctgaagccat tgtgaatgca tcaatacaga gctcctcggt
     6421 gcgtttgtca gggaatgccc tgtcaaactt tgatacacca gctgtaacta tataaacagg
     6481 gcgcataagc tttggaattt ttgattgttg ttgtgtaaat gtaatcatag gctaaacctc
     6541 tctctattat tttaatgtat atgcaccata aaaatatgca ttgtaccttg ctgatagcat
     6601 atagctatcc gtagtcatag gccattataa actaaatagt ccccacccag agagcaaccg
     6661 caatcatctt tgaattattg gtaatatgtt gctgctcagg ttgagttata aaatgtgcgg
     6721 catcgccttg atagagcaca aacacctgat tctggtaggt taattcaagc gaacctttta
     6781 aaacacaaag cacctcctgg ctattgcgta tacctttacg agcaggaata ctttttcctg
     6841 gttctaccat cagtattgca ctgtctattg ttctacgtga ttcgggtgaa aaaaaacgta
     6901 atgttgcaaa tccaacgtgt ggtatatcta tctctgtgag atcctttttc cgtatgatgg
     6961 aaattttttt ctgattttga gcctgctgaa ttacttcacc agcatcacaa ccaattgatg
     7021 aacaaatatt tataagcgta tccacggatg gagaatttac attattttct atctggctta
     7081 aaaaaccttc agagatgccg gcgcgtgatg ccactgtctt taaggtcatc tttttttctt
     7141 ttcgttttat ttttaacacc gaaccaagat ccattattag tttctcatta gtaaagattc
     7201 ataaataaaa caactattgc cattaataat atttactata agtaaatatt tataatattg
     7261 tcaataataa tttatcaatt taaaaaaatt gtattttttg gcgatagtaa cccttaatca
     7321 gtgtatgcta tagcaagaat gcactgccaa taataagtaa ctatcgccca tagtattgtg
     7381 aagattttgt agaaaggtaa tagaattttg taatgatgat agatttacaa tttttcatgg
     7441 agattgtgca atcagtatga tagatgagca tttcccctgg caagaagctg tccagtatcg
     7501 tcatgaactt caaaaaaaag atggcggttt tggtttacct gtttttttga atacagggct
     7561 atattaatgt ttcctggcgg tatcatcata cctgtaaaac gacaggataa tacctttaat
     7621 acatgtggat tgccgtcagc ttcatgattg ataatttcgc gtgcagtata cgctaacgta
     7681 gcagttccct gtagaataat atcaggcagt cctaccatct ttgcaaatgc taccgatgtg
     7741 tgtattggaa aatgaatatt agaacaacca tcataaacat aaggacgcag tctatcaata
     7801 taaacttttt tttcccagca tggtgatgat gtgtcttcag aaaaaccggg aattggaata
     7861 ttgctgtttt ccgtaacggt gccggtgcac tcaacaccgc gcaacattcc acctatatgt
     7921 tcggtaaaga ctggttgatt gttgtggctg tatgcatcaa atcgtatggt gatgatggtg
     7981 ccggcacgat gaggcattat tgaagctatt tttcctttta tgatcaatgt gtnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtgccggc acgatgaggc attattgaag
     8161 ctatttttcc ttttatgatc aatgtgtcat tgggggctat tggacggtgg aattcaagat
     8221 gttctgtata atgcacctgg gttttaatta tctcaagagg aaaattttta ttgttgatgt
     8281 aatcacaaat tttttctgat attggccatg tgcaggcaac agcaaacatt gggtgggcaa
     8341 tgatcccttc agtgctttca tcattaaaat aataaggatt gttatcatca attgctgctg
     8401 cataattcat cattgcacga gatgatatcg ttgttgtgta atcctttagc gttgttccga
     8461 caaaagatgc atcaagcttc atagtaaaaa ccttccaatt gaattaaatg cattataatt
     8521 attatattcc ttattgtaac aattgcaaca tgggatcagc tacatgataa acattaattc
     8581 ttaaataaat cattaaaaaa attttattta tcaccattcc accactcctc aagttgaaat
     8641 gtatggttaa cattaactaa ttgtccaatt tttggtataa ttaaatcaat tttatgtgtt
     8701 ttagacaaag catagagctg tttgggtgga tcattccatg gatgttttga taggatgaac
     8761 ataccccaat gaatagggaa gaatttttta gccctaagat ctttgaatgc agtgacagag
     8821 tcttcgggaa acatatgcac ttctctccat tgattgttat attggccaca ttctaaaaat
     8881 gctatatcaa atgggccgta tttttttcca acagctttaa aatggatatc atagcctgaa
     8941 tctccactaa aatagacact gtgattcttt gacttgatta cccatgaagc ccataaagtt
     9001 ttgtttcggt caaataaacc acgtcctgaa aagtgttgag caggtgttgc tatgaattgt
     9061 atactatcaa attttgcagt tacccaccaa tctctttcca caatcctatc gtgaggtatg
     9121 ccccagcttt ttaaatgtga tcctacacct aagggaacaa caaaacgtgc ttttttatca
     9181 ataaaatatt ttacagactt catgtccaga tgatcatagt gatcatgaga tattaatata
     9241 taatctatta ctggtagctc attcagcttt aataccggtg gctgaaaccg tttattcata
     9301 atatttaccg ggaatgatga gttggtaaat actggatcta ccagaacaat tatcccatct
     9361 atattaagca taaaagagga atgaccaaac cagattacac gcaaatctga atcctgttgt
     9421 aaaaatactg acagatctgg ttttagttca ggaagttttg tggttggctt tagaattttt
     9481 ctgcctttaa acatatcaat cataattgta aaggtgatat ttttatccca catctcatca
     9541 atagcatctt gattacggtt tacaaacata tttttgttag catcataatg catggaatgt
     9601 ttaaacatgc taacgttatc cctatctggg tttgttccca tagaagcaca tcctttgagc
     9661 gatattacga taataatgga tataattaat aaaataagca gctttaagaa aggctttctg
     9721 aaatttttca tagtaataaa tgtaaaattc ttatccaatg agttatccta ttttttaaac
     9781 tgttgtcgta attttcgagc atgtccctgt tttagttgaa attcatagca gttacccaag
     9841 attagtactt g
//
LOCUS       DAOOJH010000088         9832 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6147_length_9832_cov_8.579012, whole genome shotgun sequence.
ACCESSION   DAOOJH010000088 DAOOJH010000000
VERSION     DAOOJH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6147_length_9832_cov_8.579012"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="gatB"
                     /locus_tag="PLJ75_10100"
     CDS             complement(<1..1303)
                     /gene="gatB"
                     /locus_tag="PLJ75_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPD05604.1"
                     /translation="MEFDTVIGLEVHAQLNTQSKIFCSCSTQFGAQPNTQVCPVCLGL
                     PGVLPVLNKEVLKKAIMAGLALNCTVAHYSKFDRKNYFYPDLPKAYQISQYDKPICYD
                     GYIEISIKDETKKIGITRLHMEEDAGKTIHSDDANNKVSYVDFNRTGVPLIEIVSEPD
                     ITTPDEAYQYLQTLRSILKYIEVSDCNMEEGSLRCDVNVSLKPKGAKVFGQKVEIKNL
                     NSFRSVKLALEYEIGRQQKMLMQDETIVQETRLWDAERNITQAMRSKEEAHDYRYFPE
                     PDLPPIEISNDYVEQLKNELPELPYEKCDRFVKQYGLPRYDAQVLTVTRQLALYYETV
                     VSNGAHPKKASNWIMSELLAKIDDPEKIDTFIVSPQNLAILLKRIDDATISGKIAKTV
                     FEEMLKTGNDPDAIINEKGLRQVTDTAAIESIIDEVIKSNPK"
     gene            1655..2128
                     /locus_tag="PLJ75_10105"
     CDS             1655..2128
                     /locus_tag="PLJ75_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05605.1"
                     /translation="MKKKLLIIVIVVIIGVITGYGIGVFLLEKTVTGQSAFSFFITRP
                     LNIYVILNSKLYSNNPLDRLTAYYTLYDMHIVDTPFLQERYKQEENIALKRAILKTLS
                     LHADSELIQFIDDIYELSDSALKIEMVKIVKKKYPGKFAEFAQKHRVDAQWIHEE"
     gene            2200..2787
                     /gene="nadD"
                     /locus_tag="PLJ75_10110"
     CDS             2200..2787
                     /gene="nadD"
                     /locus_tag="PLJ75_10110"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPD05606.1"
                     /translation="MKVGLFGGTFNPIHIGHLINAQFIREEFNLDFILFIPTKYPVHK
                     ELEYDVDADHRYHMIKLAIEGVEYFKVSRLEIDREKPSYTIYTIDDILKENPEWQLFF
                     IIGYDAFNEIDTWKDWQRLLLTIDFIIMKRPGDILHKRFHQYMPHIHCANNPVIDVSS
                     STIRERVKNGKPIKYLLPYNVEEYIDKHGLYQSEG"
     gene            2879..3883
                     /locus_tag="PLJ75_10115"
     CDS             2879..3883
                     /locus_tag="PLJ75_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPD05607.1"
                     /translation="MLAANKELINILVAGSNDYNERRHSFYAIVHFNQEKNSIGVTFI
                     PPNYLVTLNKRTGKAMRLNMIAFSDSNDIIDSLEDDLHITIPFYLELYGSDVVKIIDL
                     IEGVQLYILDQVKRESGFMRGVHYCDGKKTLWYINKVEHDSIYIKYDRIMDVVLTLFT
                     NKESYKKYNRKSYISEMLSSLNTNLLIQEMLSLADLVYDAKTVYTTIMPGKVDSNDNY
                     TVDDITRKLYEQKFLRPLVLAVSDTESIKVRILNGSDIPGLARKMRNVLTKEGLTVVE
                     FGTSPYSVIDKTVIINQKGNLNSALRVSSILGIDAMYHIVDSSQLADVLVIAGKDYSQ
                     "
     gene            3880..4278
                     /gene="rsfS"
                     /locus_tag="PLJ75_10120"
     CDS             3880..4278
                     /gene="rsfS"
                     /locus_tag="PLJ75_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPD05608.1"
                     /translation="MTGKSKKSKKQNIDEGTVTLLKGCGKVLDDKKALDIVVLDLRNV
                     NSYLDFFVICTGNSIMHCKALARELTKYFLDSGMKERSKTTANSPWIILDYNEIIIHI
                     FTAEARAFYQLEHLWADASKIDLDENIHDG"
     gene            4256..4891
                     /locus_tag="PLJ75_10125"
     CDS             4256..4891
                     /locus_tag="PLJ75_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPD05609.1"
                     /translation="MKTYTMDEVDIIEKVIRNVAHEVRNPLTTIRGYAQLLTQKLDTT
                     SIQKSQKMIIEQAERIDGMFNDLYNAFTIKEDTIVGCLIPDILQKALDASVYHDYIII
                     DDCESFQVQCYMNRLLLCFNTFVNGFNWKYFPDARLRIYVKKETFCTIYFQFTNVIFD
                     AILESLFFYPFQTKHFFIKGTELFSIYCIAKRSRWDMDFNYNNSTFAISIP"
     gene            4821..6410
                     /locus_tag="PLJ75_10130"
     CDS             4821..6410
                     /locus_tag="PLJ75_10130"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPD05610.1"
                     /translation="MRKEVDGTWILTIITVPLPLAYRDDAIKIVYGTIMATILEKYYH
                     VLIADDEKGIRDLLKDLLEDSFYVNTIDRGDLIIDELKRKKYDILILDLQMPGKNGIE
                     VLQAIHNLKIDIVVVVITASNDVQLAITSMKLGAYDYLVKPFDTDKLLVILKNIVEKL
                     DLENEVNELREKVGESFRFKNIIGKSPKMQRIFATLERVIDTNSTILIIGESGTGKEI
                     VAKAIHYNSNRKQYPFKSIDCSTIPQDLIGSELFGHEKGAFTGAIARKIGKFEIASKG
                     TLFLDEISNLSFDMQAKLLRVLQEKEFERIGGNEIIKVETRIVAASNRDIREMVKQGT
                     FREDLYYRLNVMPIYLPPLKERKEDIPLFIDYFLKRFTSEYGRTITLDLQSRLYLTDY
                     HWPGNVRQLENVIKRLVLLSTEDVVKLDIVKSVLEFEEYNKPAVQQDVYAAIPSTIAT
                     APVHESFDNGDTISDNDTTLQDETVKTLDEVEKEYIEKMLKQFNFNISVTAKAINVSR
                     KTMHNKLKKYNIAIKKTVADD"
     gene            6611..7549
                     /locus_tag="PLJ75_10135"
     CDS             6611..7549
                     /locus_tag="PLJ75_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HPD05611.1"
                     /translation="MAILLIVIGSSLLRGFSDKESSFTRYLLNAPFNGRAGHTCTVFK
                     GKLWLIGGWDGNNPYNDVWYSKEGLHWYSAGEMPFKKRAGHTAFVFNDKLYVVGGLSF
                     DECKNIIDMNDVWVSDDGMNWQCIKETAEFMPRGGHACALFKNKVYLVGGIACGAEAY
                     KTEDGVNWNRLANKLPFGSRGGHVLTNFKNKLWLIGGIRVDRNNNFMSFSDVWNSGDG
                     IHWKKVASDMPFFAGGGHGCVVYKNSLWVIGGFRKGGTVYRSDDGMNWELFNSFTPMG
                     ERVSHSCTVFKDRIIIIGGFDGTGFKNDVWAFKTLE"
     gene            7500..8291
                     /locus_tag="PLJ75_10140"
     CDS             7500..8291
                     /locus_tag="PLJ75_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD05612.1"
                     /translation="MVQDLKMMYGRLKRWSNQSNCYYENHTRFTTIKKIIKVAIVIHI
                     AFVVLMGSIIMYYDKHNPSNTPLMYYRAYINNYTVKPVQCVPLKKIPDDIKKMVISIE
                     DYKFYKHNGVDIEAIKRALIINYKVGYRMYGASTITQQLARTLFLFPVKSMIRKYAEL
                     IIALEMEVLLDKDRIFELYLNYCEWGKGLFGIKNASYYYFGRNIHNLRNDEKSRLLAI
                     LANPIIYSPYKFKGSKLITNRYYILKFRYYTYTKYKSMMQYAITR"
     gene            8388..9788
                     /locus_tag="PLJ75_10145"
     CDS             8388..9788
                     /locus_tag="PLJ75_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPD05613.1"
                     /translation="MQNFPIQSKSFSEDTTLSRQLGLPSAIAIVISRIIGSGIFRTPG
                     PIMALVGSTLLFGSVWIIGAVITIFAAICYAELVAMMPRSGGPYVYLKEAYGPLIAFL
                     RGWAMFFVSETGAIAAVALVFAEYTVASITLVSGVVLSHAVVVMLALLVIVFHTFINC
                     FGVKLSGVVQIIISSSKIVALGYIVVSCFSNNGDIHNFYNPLVPENFTFAHILGIGAA
                     LRYAFFAFSGWEGATYVAEEVKNPRKNLPLSLFVGIACVLILYASTNAAYLFQVPVQY
                     FSQSKWIAVDALQNALGAIGALIVAYAVIINTFGNVGTQIMVKARTWHAMASDNLFFK
                     VCSTVHPEYKTPNNAIIVQGIWASILVLFASMYKDSYKAVIDYFSATGTIFNILTLGS
                     VIVLRKKYPGIVRPFKAWGYPVTVILVISFYVVYLIITFITAFAPSLLGIIFTLSGLL
                     YYWYKSAGKSKNEAPY"
BASE COUNT         3209 a         1467 c         1852 g         3304 t
ORIGIN      
        1 atttaggatt gcttttaata acttcatcaa taattgattc aatagctgca gtatcggtaa
       61 cctgacgcaa gcctttttca ttaataatag catcggggtc attacctgtt tttagcatct
      121 cttcaaagac tgttttagca attttaccac ttatggttgc atcatcaatc ctttttaata
      181 ggatggctaa attttgcggc gaaacaataa aagtatctat tttttcagga tcatcaattt
      241 ttgctaaaag ttcgctcatt atccagttag atgctttttt aggatgtgct ccgttagata
      301 caacagtttc gtaatacaga gctaactgtc tggtaaccgt taatacctgc gcatcatatc
      361 tgggcaatcc atattgttta acaaagcgat cgcatttttc gtatggaagc tcgggcaatt
      421 cattttttaa ctgttctaca taatcatttg atatttcaat tggcggtaaa tcaggttcag
      481 gaaaataacg ataatcatgt gcttcttctt ttgaacgcat agcctgggta atatttcgtt
      541 cagcatccca tagacgtgtt tcctgaacaa ttgtttcatc ctgcataagc attttttgtt
      601 gtcgtccaat ttcgtattct aatgctaatt ttactgatct gaagctgttc agatttttaa
      661 tttcaacttt ctgcccaaac acttttgctc ctttaggttt taaagaaaca ttgacatcac
      721 atcgcaagct accttcttcc atattgcagt cagatacttc aatatacttt aatattgaac
      781 gtaatgtttg taaatactga tatgcttcgt caggagtagt gatatcaggt tcagaaacaa
      841 tttctattaa aggaacacca gttcggttaa aatcgacata tgacacttta ttattagcat
      901 catcactgtg aatagtttta ccggcatctt cttccatatg aaggcgtgta atgccaattt
      961 tttttgtttc atcctttatt gatatttcaa tataaccgtc atagcatata ggtttatcat
     1021 actgcgaaat ctgatatgcc ttgggcagat caggataaaa ataattttta cggtcaaatt
     1081 tgctatagtg agcaactgta cagtttagtg caagtccagc cattattgct ttttttaaaa
     1141 cctctttgtt aagaacaggc aacacccctg gaagtcccag acataccgga caaacctgag
     1201 tattgggctg tgccccaaac tgcgttgaac aactacagaa tatttttgat tgtgtattta
     1261 gttgagcatg aacttccagt ccaataaccg tatcaaattc catacatatt gtaccatttt
     1321 tattctataa aggtgataat agctttactg atattctttt cggatagtag atagtattat
     1381 ttttgtcaac taaaattgat tttatttcta tcatatatta ttgttggcaa taatttctac
     1441 aataaaaatc ataatactat tgaaatacta aatttttgta ttaaaatgta taaaaatcct
     1501 gcaacaaagt agttagtacc ttttctattt aatgtaaaag taatgtatca ttattgacaa
     1561 aataatttaa aaaaatacta tcgcaagaag taacgttttt tatgattctt tgttgcaaat
     1621 ccattgtcta tctggtttaa catagcatca ttatatgaaa aaaaaattat tgataattgt
     1681 aattgttgtt ataataggtg taattacagg ttatggcata ggtgtttttt tgctggaaaa
     1741 aacggttacc ggacaatcag catttagttt ttttattact cgacctttaa atatttatgt
     1801 catcctgaat agcaagctgt atagcaacaa tcctcttgac agattaaccg cctattacac
     1861 cctttatgat atgcatattg tagatacccc ttttttgcag gaacgctata aacaggaaga
     1921 aaacatagct ttaaaacgcg ctattttaaa aactttatcg ttacatgctg atagtgaact
     1981 gatacaattt atagatgata tctatgaact atctgacagt gccctgaaaa ttgagatggt
     2041 gaaaattgtc aaaaaaaaat atcccggaaa gtttgctgaa tttgcgcaaa aacacagggt
     2101 tgatgctcag tggatacatg aggaataata aaatttcttg actgttataa tagtattaat
     2161 tgatgtttat ctacactaaa ttgaaaggtg gtaaaactat tgaaggttgg tttatttggt
     2221 ggaacattta atccaataca tattggacat ttgattaatg ctcaatttat aagagaggaa
     2281 ttcaatttag attttatact atttatccct acaaaatatc ccgtacataa agaattggaa
     2341 tatgatgtag atgccgatca ccgataccat atgattaaac ttgctattga aggtgttgag
     2401 tattttaagg tttcccgtct tgagattgac agggaaaagc catcgtatac catatatact
     2461 attgacgata ttttaaaaga aaatcctgaa tggcagttat tttttattat aggatatgat
     2521 gcgtttaatg agattgatac atggaaagat tggcagcgtt tacttttaac aattgatttt
     2581 atcattatga aacgcccggg cgatattttg cacaaacgat ttcatcaata catgccccat
     2641 atacactgtg ccaataatcc tgttattgat gttagctcgt caactattcg tgaacgggtg
     2701 aaaaatggta agccgataaa atatttgttg ccatataatg ttgaagaata tattgataaa
     2761 catgggttat atcaaagtga aggataaaat acggttaata gtttctgtaa ctctggtttg
     2821 catagtaatt ttaagtatat tttatattta ccgcagattt acgcgtaatg caattcaaat
     2881 gctggccgca aacaaagagt tgattaatat tttagttgct ggaagcaatg attataatga
     2941 acgacgccat tccttttatg cgatagttca ttttaatcaa gaaaagaaca gtattggtgt
     3001 aacatttata cctcccaact accttgtaac actcaataaa cggacaggca aagccatgcg
     3061 gcttaatatg atcgcgtttt cagatagcaa tgatatcatt gattcattag aagatgattt
     3121 acatataaca atccccttct atctggagtt atatggatct gatgttgtta aaatcattga
     3181 tcttattgag ggagtgcagc tatatatact tgatcaggtt aaacgtgagt caggatttat
     3241 gcgtggtgtt cattattgtg atggcaaaaa aacgctatgg tatattaata aagttgagca
     3301 tgattccata tatattaaat atgacagaat aatggatgtt gtgcttacgc tatttaccaa
     3361 taaagaatcg tataaaaaat ataatagaaa aagttatatt agcgagatgc ttagttcact
     3421 gaataccaat ctacttatac aagaaatgtt atcattggca gatcttgtat atgatgcaaa
     3481 aactgtctat acaacaataa tgccagggaa ggtagatagt aatgacaatt atacagttga
     3541 cgatattacc aggaaattgt acgaacaaaa atttttacgg cctttggttc ttgctgttag
     3601 tgatacagaa tcaattaagg tacggatact aaatggttca gatatcccag gccttgcccg
     3661 gaagatgaga aatgtattaa caaaggaagg tttaaccgtg gtcgagtttg ggacttctcc
     3721 ttattcagtc attgacaaaa cagtcattat aaaccagaaa ggaaatttaa attctgcttt
     3781 gagagtttca tctatattgg gtattgacgc tatgtatcat atagttgatt catcgcaatt
     3841 agcagatgta ttagtaatag caggaaagga ttattcacag tgacaggaaa gagtaaaaaa
     3901 agcaaaaagc aaaatattga tgaaggtaca gtaaccttgc taaaaggatg cggcaaagtg
     3961 ctggatgata agaaggcgct ggacatagta gttctagatt taagaaatgt taattcgtat
     4021 ttagatttct ttgtaatctg tacgggaaat tctataatgc actgtaaagc attggcacgt
     4081 gagctcacta agtatttttt agattcaggc atgaaggaac gttcaaaaac aacagctaat
     4141 tcgccatgga ttatccttga ttataatgag attattatac atatttttac agctgaagca
     4201 cgtgcctttt atcagcttga gcatctatgg gctgatgcat caaaaattga tttagatgaa
     4261 aacatacacg atggatgaag ttgatataat agaaaaagtt atacggaatg ttgctcatga
     4321 ggttcgcaac ccattaacta ccattagagg atatgctcag cttttgaccc aaaaattgga
     4381 tactacatca atacagaaat cccaaaaaat gattattgaa caggctgagc gtattgatgg
     4441 aatgtttaat gacctgtata acgcatttac cattaaagaa gataccattg ttggttgctt
     4501 gatacctgat attttacaaa aagcccttga tgcgtctgta taccatgatt atattataat
     4561 agacgattgt gaatcatttc aggtacagtg ttacatgaac cgattattac tatgttttaa
     4621 cacctttgtc aatggtttta actggaaata ttttcctgat gccagattgc gaatatatgt
     4681 aaaaaaagaa actttctgta ccatttactt ccaatttacc aatgtaatct ttgatgccat
     4741 tttagaaagt ttattttttt atccatttca aactaaacat ttctttataa aaggaacaga
     4801 attgttttct atatattgca ttgcgaaaag aagtagatgg gacatggatt ttaactataa
     4861 taacagtacc tttgccatta gcataccgtg atgatgctat taaaattgtt tatggaacta
     4921 ttatggcaac aatattagaa aaatattacc atgtgctgat tgctgatgat gaaaaaggaa
     4981 tcagagattt attaaaggat ttattagaag attcatttta tgtcaataca attgaccgtg
     5041 gtgatttaat catagatgaa ctgaaaagaa aaaaatatga tattttaatt cttgatctgc
     5101 agatgcctgg taaaaatggt attgaagttt tacaggctat acataattta aaaattgata
     5161 ttgttgtggt agtcataact gcttcaaatg atgtgcagtt agctattact tctatgaaat
     5221 taggggctta tgattatctg gtaaaaccgt ttgatactga caaactttta gtaatattaa
     5281 agaatatcgt tgaaaagctt gatcttgaaa atgaagtaaa tgaattacgt gagaaggttg
     5341 gtgaaagctt caggtttaaa aatataattg gtaaatcacc taaaatgcag cgtatctttg
     5401 ctactcttga gcgggtcatt gacactaata gcactatatt aattattggt gaaagtggta
     5461 ctggcaaaga aattgttgca aaggcaattc attataacag caaccggaaa cagtatccct
     5521 ttaagtctat cgattgcagc accataccgc aggatttgat tggcagtgaa ttatttggtc
     5581 atgaaaaagg tgcatttacc ggagctatcg cccgaaaaat tggcaaattt gaaattgcaa
     5641 gcaagggaac cctatttctg gatgagatta gcaatctttc gtttgatatg caggcaaagt
     5701 tgctgcgtgt actgcaggaa aaggaatttg agcgtattgg tggcaatgag ataatcaagg
     5761 tggagacacg tatcgtagca gcaagtaacc gggatatccg ggagatggtt aaacagggaa
     5821 cattcagaga agatttgtac taccggctta atgttatgcc tatttatctt ccaccgttaa
     5881 aagaacgcaa agaggatatt cctcttttca ttgattattt tttaaaacga tttaccagtg
     5941 aatatggtag aacaattact cttgatttac aatcgcgatt atatttaacc gattaccact
     6001 ggcctggcaa tgtgcgacaa ctggaaaatg tcatcaagcg tcttgtattg ctttcaacag
     6061 aggatgttgt aaagctggat attgttaaaa gtgtactgga gtttgaagaa tataacaaac
     6121 cggcagtgca acaagatgta tatgctgcca taccctcgac tatagcaacg gcacctgttc
     6181 atgaaagttt tgataatggc gatactatta gcgataacga tactaccttg caggatgaaa
     6241 cggtaaaaac acttgatgaa gtagaaaaag aatatattga gaaaatgtta aagcagttta
     6301 acttcaacat atcagtaacg gcaaaagcta ttaatgtttc cagaaaaaca atgcataata
     6361 aattgaaaaa atataatata gcgataaaaa agactgtagc tgatgattaa ggaataatga
     6421 agaaattatt atacaatagg tcgcaaagaa tgttaaattt cagaagaact tcccttcatt
     6481 tttgatcatt aatactacgg attataatga tagtgttatt attttttgtt aatagctaat
     6541 ttattaatag agtatataat acaatatgaa taagggtaca tatatatata aatttgtttt
     6601 tggaactcta atggctattc ttttaattgt cataggttcc tcgctactaa gaggatttag
     6661 tgataaggag tcaagtttta cgcgctatct tcttaatgct ccttttaatg gacgtgccgg
     6721 acacacctgt acagtattta aaggtaaatt atggcttatt ggcggctggg atggtaataa
     6781 tccatacaac gatgtatggt attcaaaaga gggtcttcac tggtatagtg ctggagagat
     6841 gccttttaaa aagcgggccg gacacactgc atttgttttt aatgataagc tgtatgtggt
     6901 aggtgggctt tcatttgatg aatgtaaaaa tattattgat atgaatgatg tgtgggttag
     6961 tgatgatgga atgaactggc agtgtataaa agaaactgct gagtttatgc cgcgaggtgg
     7021 acatgcctgc gctttattta aaaataaagt ctacttagta ggtggtatag catgcggtgc
     7081 cgaagcatat aaaaccgaag atggtgtaaa ctggaacagg cttgcaaaca aactaccttt
     7141 tggttctcgg ggtgggcatg ttttaacaaa tttcaaaaac aaattgtggc tgattggtgg
     7201 aatacgtgtt gacagaaaca ataattttat gagctttagt gatgtatgga attcaggtga
     7261 tggaattcac tggaaaaagg tagcaagcga catgcccttt tttgctggtg gcggtcatgg
     7321 ctgtgttgtt tataaaaatt ctttatgggt aatcggtggt tttagaaaag ggggtacggt
     7381 gtatcgctct gatgatggaa tgaattggga attatttaac agctttactc ccatggggga
     7441 acgcgtttca catagttgta cagttttcaa agacaggata attataatag gtggttttga
     7501 tggtacagga tttaaaaatg atgtatgggc gtttaaaacg ctggagtaat caaagtaact
     7561 gttattacga aaatcataca cgtttcacta ctattaaaaa aatcataaaa gtggcgatag
     7621 ttattcatat tgcctttgtt gtgttaatgg gatcaataat tatgtattat gacaagcata
     7681 atccttcaaa tactccattg atgtactatc gggcatatat aaacaattat acagtgaaac
     7741 cagtgcaatg tgtaccctta aaaaaaatac ctgatgatat aaagaaaatg gtaatctcaa
     7801 ttgaagatta taaattttat aaacataatg gtgttgatat tgaggcaatt aaacgagctc
     7861 tcatcattaa ttataaagtt ggttacagga tgtatggagc cagtaccatt acacagcagt
     7921 tagcacgaac gttgttttta ttcccggtaa agtcgatgat cagaaaatat gctgaattaa
     7981 ttatagcttt agagatggaa gtattattag acaaagacag gatttttgaa ttgtatctta
     8041 attattgcga atggggaaaa ggtttatttg gcataaaaaa tgcatcctat tattatttcg
     8101 gaagaaatat tcacaattta cgtaatgatg aaaaatctcg cctgctggct atacttgcaa
     8161 atcccattat ttattcccca tataagttta aaggttcaaa acttataacc aatcgatatt
     8221 atatattaaa gtttagatac tatacctata ctaaatataa aagtatgatg cagtatgcta
     8281 taacccggta aaaaatttgc actatgcaag ctcttatgga tatgaaagat aatacaaaaa
     8341 tgattgtgga tatcagcagc tatttgaccg cttacacgat actatagatg caaaatttcc
     8401 ccatacaaag taaaagtttt tctgaagata ctacgttatc acggcaattg ggattaccat
     8461 cagctattgc tattgttatt agccgtatca tagggtcggg aatatttcgc acaccaggtc
     8521 caattatggc tctggttggc tctacattgc tttttggaag tgtatggata attggtgctg
     8581 ttattactat ttttgctgct atttgctatg ccgaactggt tgcaatgatg ccacgctccg
     8641 ggggtccata tgtttattta aaagaagcat atggcccatt aattgcattt ttacggggat
     8701 gggcaatgtt ctttgtttct gaaacaggtg ctatagcagc ggttgcttta gtttttgctg
     8761 aatatacggt tgcttcaatt actcttgttt ctggagttgt gctatcccat gcagttgtag
     8821 taatgctggc gcttcttgtc attgtttttc atactttcat taattgtttt ggcgtaaaat
     8881 taagcggtgt tgtccagata ataataagtt cttcaaaaat agtagcactg ggatatattg
     8941 ttgtttcttg cttttccaat aatggcgata ttcataattt ttataatcct cttgttcctg
     9001 aaaattttac atttgcgcat atattgggaa taggtgctgc tctccgatat gcattttttg
     9061 ccttcagtgg atgggaaggt gctacgtatg ttgcagaaga ggttaaaaat ccccgtaaaa
     9121 atctaccgtt atcattattt gttggtattg catgtgtact tattttatat gcaagcacta
     9181 atgcagcata tctttttcag gtgccggttc agtatttttc tcagtcaaaa tggattgcgg
     9241 ttgatgcatt gcaaaatgca ttgggtgcca ttggagcatt gattgtagcg tatgcagtca
     9301 ttataaacac ttttggaaat gttggcactc agataatggt gaaagccagg acctggcatg
     9361 ctatggcatc agataatctt ttttttaagg tttgttctac ggttcatcct gaatataaaa
     9421 cccctaataa tgcaataatt gtacagggaa tatgggcaag catacttgtc ctttttgcat
     9481 ctatgtataa agattcatat aaagctgtta ttgattattt ctcggcaacc ggaacaattt
     9541 tcaatatttt gacattaggc agcgtaattg ttttgcggaa aaaatatccc ggtattgtac
     9601 gaccgtttaa agcatggggt tatcctgtta cggttatact ggtaatttca ttctatgtag
     9661 tatatttaat tataacattt ataactgcat ttgcaccatc tcttctggga ataattttta
     9721 cattatctgg actgctttat tactggtaca aatctgcagg gaaaagtaaa aatgaagctc
     9781 catactagtt attatgtcaa aatactattt aaagagtttt aattaacagg at
//
LOCUS       DAOOJH010000089         9744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6204_length_9744_cov_9.099907, whole genome shotgun sequence.
ACCESSION   DAOOJH010000089 DAOOJH010000000
VERSION     DAOOJH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6204_length_9744_cov_9.099907"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3976)
                     /locus_tag="PLJ75_10150"
     CDS             complement(<1..3976)
                     /locus_tag="PLJ75_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD05614.1"
                     /translation="MNFNNLKYLISLFKFDFLTTRQRRFIYLLIVLLIIAGLSFVIIE
                     YKETKIGLKNELKNLTETIEIDINSWITDQIDDAQLLVNSQYFKYCLLSYLKYHESTT
                     RKQLTNFLSFYALNNDHSEVLIVDSNNNIIFTLFNTINSLSIQSIESLKHAAQVNRTV
                     ISDIQKDEKNAFYYISIITPFHINNNTNPLFVVLITYAHNFSLPILTAGTTSRFDVRK
                     MMIDTSQHAVYIFRNDSNTKTENITIYNLNSNNFKYAIHSQSNFIITNDLNNKKVIAY
                     KKLISNTDWILVTALPYWNVFSSWYKIAWLSVLIIILSFVFILSFVVLINKNNEKYFY
                     QKLYENNQKQLQQYLQYKLMLESIGDGIICTDSNGKIQIFNYIAELLTGYKFDEIKNE
                     HVNKILNIVDKTKIDRQHDIFKSILDGSYSGDYLHSVLISKDGIIVPINYKGTVIKDN
                     EGKVLGAIIVIQDQTKESFLQLLSEIRIRFVNYAIEHTRDEFIHHALKNLADLTDSFA
                     GCFIGYNYYFNKTNDFNQYLLPDFCNIYCADTTTEISIDYNSVWQQCFECKSVIINDS
                     NTSYFSLNNNQNKKDKHELFIPIIDNNNTVAVIGFANITREYTENEKSKIMILADILY
                     EMIVYKEKEETYKNLVTLTNDLVCIIDLHNATFILVNPSFVKILGYSEEELKSKSFVE
                     FIHPEDVEKTLGIMNNTLLAGENIETFENRYRCIDGTYRWLDWNAHPIASMGLAFAIA
                     RDVTERKQLEDILRKSEEKFKNLFIHSNDGISLHQLVYDNNGNPVDYIILDVNPKYEE
                     MLSINKNEAIGKRSTEVYKQATPPYFDIYKEVTLTGNPTVFETFYEPAGKYFIISVYS
                     PQKGQFATIFKDITPFKKSEQELQESEKKFSLFMNNLPLVVVIRDIMGHYVFMNDKWE
                     DAMDLKKEDWLGKTPFDVFPEEDAKKLIQIDHDAIVNGTTEPIELTLHHKTGIKHWLA
                     NRFVIYNTNNKPVYVATLYIDISEKQKVEEERERLKDQLIQAQKLESIGRLAGGVAHD
                     YNNMLEVIMGNAQLAMMQLENTAELKIFLNEILNASRRSAEITRQLLTFARKQITEPQ
                     IVNVNFVIETMLKMLGRLIGENIELIFNPENKIWNILIDPTQINQIIANLCINARDAI
                     DANGIITITTNNVTLDEYYCSQAPDCLPGEYVMISVNDNGCGMDKEIMDKIFDPFFTT
                     KEFGKGTGLGLSTVYGIVKQNNGFIKVYSEKGSGSTFNIYFPRYVTENEYQETEIIDD
                     KPLGNGETVLVVEDEQSLLKMIQIMLERLQYNVIAVSSPLEAIELVKSRGNEIALMLT
                     DLVMP"
     gene            complement(4147..4956)
                     /gene="purQ"
                     /locus_tag="PLJ75_10155"
     CDS             complement(4147..4956)
                     /gene="purQ"
                     /locus_tag="PLJ75_10155"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPD05615.1"
                     /translation="MVKPKALIVTGYGINCDEETKFAFALAGADSKIIHINDIIENRS
                     ILKNYHIFAFPGGFSYGDDTGSGKALANKIKNNLMDEMLSFIQRDTLMIGICNGFQVM
                     VNLGIVPALTNSIGSVEVALEHNASFRFQCRWIDLTINSNSNCIFTQGITRLHIPVAH
                     GEGNFIAPHNIVDKLVQNNQIIMRYSQPDGTPANGEFPFNPNGSMLDVAALCDTTGRI
                     MGMMPHPERHIYFMQRDDWTYLREIAIREKTDIPEYGEGLQIFKNAVKYFQ"
     gene            complement(4965..7952)
                     /locus_tag="PLJ75_10160"
     CDS             complement(4965..7952)
                     /locus_tag="PLJ75_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPD05616.1"
                     /translation="MEYAPFRIEVYYTIPDSRGTVLQKKLKYSGYSVDKAIRTDNYLI
                     NIQLSEDEIVKVAQSLIQPVTQNYTINKAFAPEFFTYAIEIGYLPGVTDNVAHTTREM
                     IEDIIKKPLNPEKSVFTSCTFFIWGTIDNHSIEDISKELYNPLIQRATILSYNDYVLQ
                     NGMGNAIPVVTLHEDTKAEIVSLDISNEELVALGKEGIKNPDGTRRGPLALDMLSLET
                     IKKYYKEIEKRNPTDVELESIAQTWSEHCKHTIFAAQLDEITDGIFKHYIKEATVRIR
                     REKGKDDICVSVFSDNAGGIEFDDKYVIADKVETHNSPSALDPFGGAITGIVGVNRDI
                     IGFGLGAQPVANRYGFCFANPFDTKPLYKTKDKNSKMLSPRRIMEGVIHGVNVGGNCS
                     GIPTPQGFVYFDDRYKGKPLVFVGTIGLIPKKINGKNSVHKKAMPGDNIVMVGGRVGR
                     DGIHGATFSSEALTSGSPATAVQIGDPITQKKLSDAIVKEARDMGLYHSITDNGAGGL
                     SCSVSEMAREAGGFIVYLDKVPLKYHGLSPWQIWVSESQERMTLSVPDETLDDFMQLM
                     KKRGVEAWVIGRFTDSGRGIVYYNGEIVFNIDLQFLHEGLPQKKLTGIYEKKLHPFPE
                     FSDSINATDDLCAMVKRLNTASFEFISTQYDHEVQGNSVIKPLQGKGKVNGNASVIRP
                     VLQSKRGVVLSQGVYPSYSEIDAYWMAACSIDTAIRNAVACGCDFDKLALLDNFCWCD
                     SSNPHRLGQLKEAARACYDFAVAYNTPFISGKDSMFNDFKGYDEHFNEVFISIPPTLL
                     ISSIGIVSDIAYCQTIDFKKPGDLVYMLGITKDEMGGSEFLAYKGEQIKGEKYIGDSI
                     PEVDAMLHEKIYRIIEKLLRKNLIASSISVERGGLALAVAKSAMSGLVGVTIDLTKVL
                     HENVMRNDTMLYSESQGRIIVSIAPENKEQFEKLCCDIPFARIGRVKDDQYITITGID
                     GNEIISVSVCQLHAHYKSTFSEF"
     gene            8313..8603
                     /locus_tag="PLJ75_10165"
     CDS             8313..8603
                     /locus_tag="PLJ75_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05617.1"
                     /translation="MKAGLLISGSGALIYLTSYDSFMNDKLKEKFAAKGINKFIAYEI
                     PVEEAKKRYGGHFEIVVRDLHESDDLRILDYDGSRAFKLFSFQELGKPVYQE"
     gene            complement(8644..>9744)
                     /locus_tag="PLJ75_10170"
     CDS             complement(8644..>9744)
                     /locus_tag="PLJ75_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPD05618.1"
                     /translation="AIPVVTIVLGILTAYGLAGGFASDPHAVSRGLYGIAFAAVGMLS
                     TLGITLATDAYGPIADNAGGNAEMSGLGKEVRTKTDALDMLGNTTAAIGKGFAIGSAA
                     LTAMALIAAYIEEVKMWISKYAAQSVDGFFQVGAIKFYNIMPENVVADLNNVLIRNAT
                     IGDFVRAYDITLLNPMVIAGLFLGAMMAFLIAALTIKAVGKAAGAMVEEVRRQFKEFP
                     GILKGTQKPDYARCVMISTAGAQRKMILPSMLALIVPIAAGLLLGVAGTTGVLAGALA
                     AGFSLACMLNNSGGAWDNAKKFIEKGNYGGKGSDPHKAGVIGDTVGDPFKDTSGPSLN
                     ILLKLMSMVSVVFAGVVVHFYPQIAKLLGLMK"
BASE COUNT         3093 a         1817 c         1447 g         3387 t
ORIGIN      
        1 cgggcattac aagatcggta agcattaatg caatctcatt acctcttgat tttaccagtt
       61 caatagcctc tagaggagaa ctaactgcta taacattata ctgtaatctt tcaagcatta
      121 tctgtatcat cttcagtaat gattgttcat cctctaccac aagcactgtt tcaccattac
      181 ctaaaggctt atcatcaatg atttctgttt cctgatattc attttctgtc acatatcgtg
      241 ggaaatatat attgaaggta cttccactac ctttttcgct atatacttta ataaaaccat
      301 tattttgttt tactatacca tatactgttg ataaacccag tccagtgcct ttaccaaatt
      361 cttttgttgt aaagaaggga tcaaatattt tatccatgat ttccttatcc ataccacagc
      421 cgttatcatt taccgatatc attacatatt cacctggtaa acaatcggga gcctgcgaac
      481 agtaatattc atcaagtgta acattattgg ttgttatggt tattatgcca ttagcatcta
      541 ttgcatcgcg ggcattaata cacaaattag caataatctg gtttatttgt gtgggatcta
      601 ttaaaatatt ccatattttg ttttcgggat taaaaattag ttctatgttt tcgccaatta
      661 agcgacccaa catctttagc atggtttcaa ttacaaaatt tacattaact atctgtggtt
      721 ctgttatttg tttacgggca aatgttaaga gctgtctggt tatttctgct gaacgtctgg
      781 atgcgtttaa tatttcattt aaaaatattt ttaattccgc tgtattttct aattgcatca
      841 ttgctaattg tgcattcccc ataattactt ccagcatgtt attgtaatca tgtgcaactc
      901 caccagcaag ccttcctatt gattctaatt tttgagcttg aattaattga tctttcaacc
      961 gttccctttc ctcttcaact ttttgttttt cgcttatatc aatatacagg gtagcaacat
     1021 acacaggttt attattagtg ttataaatca caaaacggtt tgccagccag tgtttaatac
     1081 ccgttttatg gtgtaaagtc agttcaatag gttctgttgt accgtttaca attgcatcat
     1141 gatctatctg gatcaatttt tttgcatcct cttcaggaaa tacatcaaat ggagttttgc
     1201 ccagccagtc ttcttttttt aaatccatag catcttccca tttgtcattc ataaagacgt
     1261 aatggcccat aatgtctcta ataacaacta caagtggtag attattcatg aataacgaaa
     1321 atttcttttc tgattcctgc aactcttgtt ctgatttttt aaacggtgta atatctttaa
     1381 atattgtagc aaattgcccc ttctgaggtg aatacacaga aattataaaa tatttaccag
     1441 caggttcata aaatgtttca aaaactgttg gattaccggt taatgttact tctttgtata
     1501 tgtcaaaata aggtggtgtt gcctgtttat aaacttctgt tgagcgtttg ccaatagctt
     1561 cgtttttatt tattgacagc atttcctcat atttaggatt tacatctaga ataatgtaat
     1621 ccactggatt tccgttatta tcataaacaa gctgatgtag actaataccg tcatttgaat
     1681 ggataaataa atttttaaat ttttcttcgc ttttacgcaa tatatcttct aattgttttc
     1741 tttctgttac atcacgtgct atagcaaacg ctaaacccat agaagctata ggatgagcat
     1801 tccaatccag ccatctgtat gttccatcta tgcaacgata tctattttca aatgtttcta
     1861 tattttctcc cgccagcaat gtattgttca taatccctaa tgtcttttca acatcttcag
     1921 gatgtataaa ttctacaaat gatttcgatt ttaattcttc ttcgctgtat cccaaaattt
     1981 ttacaaaaga tggatttact aaaataaatg tagcattatg taaatcaatt atgcacacta
     2041 aatcatttgt taaagtgaca agatttttgt atgtttcttc tttttcttta tatacaatca
     2101 tttcatatag tatgtctgcc aatatcatta ttttgctttt ttcattttca gtatactccc
     2161 ttgttatatt ggcaaagcca attactgcaa cagtattatt attatcaata atagggataa
     2221 ataattcatg cttatctttt ttattttgat tattgtttaa agaaaaataa gatgtattgg
     2281 aatcattaat aataacactt ttacattcaa aacattgttg ccatacactg ttatagtcaa
     2341 tagaaatttc agtagtagta tctgcacaat atatattgca aaaatcaggc agtaaatatt
     2401 gattaaaatc atttgttttg ttaaagtaat agttgtaacc aataaaacaa cctgcaaaac
     2461 tatcggttaa atcggccaga ttttttaatg catggtgaat aaattcatct ctggtatgtt
     2521 caatggcata gttaacaaaa cgtattctta tttctgaaag aagttgtaaa aagctttctt
     2581 ttgtttgatc ctggataacg ataattgctc ctaacacctt gccttcatta tccttaataa
     2641 cagtgccttt ataattgatt gggactatta tgccatcttt tgatattaac acactgtgaa
     2701 gataatcgcc actgtagctt ccatcaagaa ttgatttaaa aatatcatgc tgtctgtcaa
     2761 tttttgtttt atcaacgata ttgagaattt tattaacatg ttcatttttt atctcatcaa
     2821 atttataacc cgttaaaagc tcagcaatgt aattaaaaat ttggattttt ccattactat
     2881 cagtacatat tattccatca ccaattgatt ctaacatcaa cttatattgt aaatattgct
     2941 gtaattgttt ttgattattt tcataaagtt tttgataaaa atacttctca ttatttttat
     3001 taattaaaac aacaaatgaa agaatgaata caaacgatag tatgatgatt aaaactgata
     3061 gccatgctat tttataccag cttgaaaaaa cattccagta tggtaaggct gttacaagta
     3121 tccaatctgt attggatatt aattttttat aagctataac ttttttatta tttaaatcat
     3181 ttgtgataat aaaatttgat tgtgaatgta tagcatattt aaaattattt gaatttaaat
     3241 tataaattgt aatattttct gttttagtat tgctatcatt tctaaatata taaactgcat
     3301 gttgactggt atcaatcatc attttcctta catcgaaccg tgatgtagtt ccagcggtta
     3361 atattggtaa agaaaaatta tgcgcataag tgattaaaac tacaaaaagc gggttggtgt
     3421 tattatttat atgaaaaggt gttattatgc ttatgtaata gaaagcattc ttttcatctt
     3481 tctggatatc actaataaca gtcctgttaa cctgagcagc atgttttaat gattcaattg
     3541 attgaatgga taatgagttt attgtattaa ataaagtaaa tataatgtta ttattgctat
     3601 caactattaa tacctctgag tgatcattat ttaatgcata aaatgataaa aaattggtta
     3661 gttgttttcg agttgtgctt tcgtgatatt tcaaataaga tagcaagcaa tacttgaaat
     3721 attgggaatt gaccagcaat tgtgcatcat caatctgatc ggttatccag ctattaatat
     3781 caatctcaat tgtctctgtt aaatttttga gttcattttt taaaccaatt tttgtttctt
     3841 tatactctat gataacaaaa gaaagccctg caataattaa tagtactatc aataaataga
     3901 taaatcttct ttgccttgta gtaagaaaat caaatttaaa caatgaaatc aaatatttta
     3961 aattgttaaa attcatcatt gtcatttcaa aaaaactttt acaaaatatt acaattaatg
     4021 attaatgata ttccatcata taatagatat attagttcat agtattgttt taaaaatata
     4081 atagtattta aacaaagtca aaataaattt ttatttaaaa aataggtgaa atgagaatga
     4141 tgtaaattac tggaaatatt taactgcatt tttaaatatc tgcaatcctt cgccatattc
     4201 aggtatgtct gttttttctc gtattgctat ttcacgcaga tatgtccagt catctcgttg
     4261 cataaaatag atatgccgtt caggatgagg catcattccc ataatccttc cggttgtatc
     4321 acacagtgcg gcaacatcaa gcattgaacc gtttggatta aagggaaatt cgccatttgc
     4381 cggtgttcca tccggttggc tataacgcat gataatctga ttattctgaa caagtttatc
     4441 tactatattg tgaggagcta taaaattacc ttcaccatgt gcaaccggta tatgcagccg
     4501 ggtaattccc tgtgtaaata tgcaatttga attggaatta atggttaaat ctatccatct
     4561 gcattgaaat cggaatgatg cattgtgctc taacgcaact tcaacactac caattgaatt
     4621 tgtcagagca ggaactatcc ctaaattaac catcacctga aaaccattac agattccaat
     4681 catcaacgta tcacgctgaa taaacgatag catttcatcc attaaattat ttttaatctt
     4741 atttgccagt gccttaccac tacctgtatc atcaccatac gaaaagccgc ccggaaatgc
     4801 aaagatgtga taatttttaa gaatagatct attttctata atatcattta tgtggattat
     4861 ttttgaatca gcacctgcta atgcaaacgc aaactttgtt tcttcatcgc agtttatacc
     4921 gtatccagtt actattaaag cctttggttt taccattgct gcctctaaaa ttcactgaaa
     4981 gttgatttgt aatgagcatg cagttggcac actgaaactg aaattatttc attaccatcg
     5041 atgcctgtta ttgttatata ttgatcgtcc ttcacccgtc ctatacgtgc aaaaggtata
     5101 tcgcagcata gcttttcaaa ctgttcctta ttttcaggag ctatcgatac tataatcctt
     5161 ccctgtgatt ctgaatataa catggtatca tttcgcatta cattttcatg gagaactttt
     5221 gtaaggtcga tagttactcc cacaagtcca ctcattgcgg atttagctac agctaaagcc
     5281 aatccaccgc gttccacact gatacttgat gcaattaaat ttttacgtaa cagtttttca
     5341 atgattcgat atattttttc atgaagcatg gcatcaactt cagggatact atcgccaata
     5401 tatttttcac cttttatttg ttcaccctta tatgctaaaa actcacttcc tcccatctca
     5461 tcttttgtaa tgccaagcat atacaccaga tcgcctggtt ttttaaaatc aatggtttga
     5521 cagtatgcaa tatcagaaac aataccaata gatgaaatca acagggtagg aggaatagat
     5581 ataaatacct cattaaaatg ttcatcatac cctttaaagt cattgaacat gctatccttg
     5641 ccgctgataa acggcgtgtt atatgcaaca gcaaaatcat aacaggcgcg tgcagcctcc
     5701 ttaagctgcc ccaacctatg cggatttgat gaatcacacc agcaaaaatt atcaagcagt
     5761 gcaagtttat caaaatcgca accacaggca acagcattgc gaatagcagt atcaattgaa
     5821 catgcagcca tccaatatgc atctatctcg ctatacgaag gatatacgcc ttgcgataaa
     5881 acaacaccac gctttgattg tagcacagga cgaatgacac tagcattacc gttaaccttc
     5941 cctttaccct gcaatggttt aataacgctg tttccctgaa cttcatgatc atactgtgtt
     6001 gaaataaatt caaagcttgc agtattcaaa cgtttaacca tagcacacag atcatcggta
     6061 gcatttatac tgtcgctaaa ttcagggaaa ggatgcaatt ttttttcata aatacctgtc
     6121 aatttttttt gtggaagccc ttcatgtaaa aactgaagat caatattaaa aactatctca
     6181 ccattataat atacaatacc tctgcctgaa tcagtaaacc tgccaatgac ccatgcttca
     6241 acaccacgtt ttttcatcaa ttgcataaaa tcgtcaagcg tttcatcagg taccgataat
     6301 gtcatacgct cctgtgattc ggatacccat atctgccatg gcgatagtcc atgatatttt
     6361 aacggaactt tatcaagata cactataaaa ccacctgctt cacgtgccat ctcagatacg
     6421 gaacatgata gtcctccagc gccattgtcg gttattgaat gatacaatcc catatcacgc
     6481 gcctctttaa caatggcatc cgaaagtttt ttctgggtaa taggatcgcc tatctgaacc
     6541 gctgtagcag ggctgcccga tgttaatgct tcagaagaaa atgttgcacc atgaatgcca
     6601 tctctcccta cccttccacc caccatgacg atgttatcgc ctggcatagc ttttttatgt
     6661 actgaatttt tgccattaat tttttttggg attaacccaa tagttccaac aaacaccaat
     6721 ggttttcctt tatatcggtc atcaaaataa acaaatccct gaggagttgg aattcccgag
     6781 cagttgccac caacatttac accatggatg actccctcca taattcttct tggcgatagc
     6841 atctttgaat ttttatcttt tgttttatac aatggcttcg tatcaaaagg attggcaaag
     6901 caaaaaccat atcggttagc aacaggttga gctcccaatc caaaaccaat aatatccctg
     6961 tttactccga caatgccagt aatagctcca ccaaaagggt ccagagcact tgggctatta
     7021 tgtgtttcaa ctttatcggc aataacatat ttgtcatcaa attcaattcc acccgcatta
     7081 tctgaaaaca ctgaaacgca tatatcatct tttccttttt cacgtcgtat tcttaccgtt
     7141 gcttctttta tatagtgttt gaaaatacca tcagttattt catcaagctg tgctgcaaag
     7201 atggtatgct tgcaatgttc actccacgtt tgtgctattg attcaagctc aacatcagtg
     7261 ggatttcttt tttcaatttc tttataatat ttttttatag tttccagtga aagcatatcc
     7321 agtgcaagag gcccacggcg tgttccatca ggatttttta ttccctcttt acctaacgct
     7381 acaagctctt cgttggatat atctaatgaa acaatctcag cttttgtatc ttcatgcagg
     7441 gtaacaacag gtattgcatt acccatccca ttttgcagta cataatcatt atagcttaaa
     7501 atagttgctc gctgaatcaa tggattgtat aattcttttg atatatcttc tatagagtgg
     7561 ttatcaatag taccccatat aaaaaaggta catgaggtaa atacgctttt ttcaggattc
     7621 agcggcttct tgatgatatc ttctatcatc tctcgtgttg tatgagcaac attatcggta
     7681 actccaggaa ggtatccaat ctcaatggca taggtaaaaa attcaggggc aaatgcttta
     7741 tttatagtat agttttgtgt tactggttgt atcaaagatt gagcaacctt gacaatttca
     7801 tcttctgaaa gctgaatatt tataagatag ttatcggtac gtatagcctt atcaacagaa
     7861 tatcctgaat attttaattt tttttgtaag acagtacctc gtgaatcagg gatggtatag
     7921 taaacttcta ttcggaaagg agcatattcc atgatacaac ctcttataaa tgtatatgtt
     7981 tcttctgaat ataatgtaat ctataaaaat caagcattaa aagtaaaagc acaccaataa
     8041 atggtgcgct ttattgttct atctttagct gctcggcatg tatcagtttt gttcaatgct
     8101 aaagatacga agtaatacaa aaacaatata tcaatgattg atacattatc taaaaataat
     8161 tatgataaat ttataaacgt taatagtaat agtcatgttt aaaaattata gctatctaaa
     8221 aaatatattt attatataac atcattgatt tatgtattat ttttacatat atttaataaa
     8281 aataatacta tttttaacaa ggaggttgtg ccatgaaagc tggacttcta atatcaggta
     8341 gtggtgcttt aatctatcta acttcatatg atagttttat gaatgacaaa cttaaagaaa
     8401 aatttgctgc taaaggtatt aataaattta ttgcttatga aattcctgtg gaagaagcaa
     8461 aaaaaaggta cggtggtcat tttgaaatag tggtaagaga tttacatgaa tctgatgatt
     8521 taagaattct tgattatgat ggttcaagag cattcaaatt gttttcattc caggaattag
     8581 gaaaaccggt atatcaggaa taataaaaaa aggggctttg taaagcccct ttttttattt
     8641 taattatttc attaatccta acagtttagc aatttgtgga tagaagtgaa caacaacacc
     8701 agcaaaaact actgatacca tactcataag ctttaaaaga atattaagtg aaggtccaga
     8761 agtgtcctta aacggatcgc caacagtgtc accaataaca ccagctttat gcgggtcaga
     8821 tcctttccca ccatagttgc ccttttcaat aaatttcttt gcattatccc atgcaccacc
     8881 agaattattg agcatacaag caagtgagaa tccggctgcc agagcacctg ccaatacccc
     8941 tgttgttcct gcaacaccca gcagtaaacc agcagctata ggtacaatta aagccaacat
     9001 tgaaggaagt atcatcttcc tttgtgcacc tgctgttgaa atcataacac aacgggcata
     9061 atctggtttc tgagtacctt tcaagatacc agggaattcc ttaaactgac gacgtacttc
     9121 ttcaaccatg gcacccgctg ctttcccaac tgctttaata gtaagagctg caattaagaa
     9181 agccatcatt gctcccagga acaatccagc tataaccatt ggatttagca gtgtaatatc
     9241 gtatgccctt acaaaatcac caattgttgc atttctaatt aagacattat ttagatcagc
     9301 tactacattt tctggcataa tattataaaa tttgatagcg ccaacctgga aaaatccatc
     9361 aacactttgg gcagcatatt tgcttatcca catctttact tcttctatgt acgcagctat
     9421 aagcgccata gcagtaagag ctgcagaacc aatagcaaaa cctttcccta tcgcagcggt
     9481 ggtattaccc agcatatcaa gagcatcggt cttagttctt acttctttac ccaaaccact
     9541 catttcagca ttgccaccag cattatcagc tattgggcca taagcatctg ttgccagtgt
     9601 gatacctaat gtggagagca taccaactgc agcaaatgca ataccataaa gccctcttga
     9661 tactgcatgt ggatctgatg caaatcctcc tgccaatcca taagcagtta aaatacctaa
     9721 tacaatagta acaaccggta ttgc
//
LOCUS       DAOOJH010000090         9533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6340_length_9533_cov_10.360519, whole genome shotgun sequence.
ACCESSION   DAOOJH010000090 DAOOJH010000000
VERSION     DAOOJH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6340_length_9533_cov_10.360519"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            954..1931
                     /gene="hemB"
                     /locus_tag="PLJ75_10175"
     CDS             954..1931
                     /gene="hemB"
                     /locus_tag="PLJ75_10175"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPD05619.1"
                     /translation="MMHPYYRPRRLRENEIIRDLIAETKPDYNKLIMPYFVIEGAGIT
                     QAIESMPGIQRLSIDNLLKQLDYDVKLGVRAIILFGIPYDKDEKASGAYDENGIVQKA
                     CREIKKQFPQLLIITDVCLCEYTSHGHCGMVKGNQIDNDSTLELLAKTAVSHARAGAD
                     IIAPSDMMDGRVETIRQELDDNGFITIPILSYAAKYASAFYGPFRDAAGSAPQFGDRR
                     SYQMDYRNAHEALKEVNLDIDEGADIVMVKPALSYLDIIYRVKQSVELPVCAYNVSGE
                     YSMVKAAGTLGFGDEAKLVREIMTSIFRAGADMVISYHTCDILKNRWFD"
     gene            1947..3233
                     /gene="hemL"
                     /locus_tag="PLJ75_10180"
     CDS             1947..3233
                     /gene="hemL"
                     /locus_tag="PLJ75_10180"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HPD05620.1"
                     /translation="MITSKSKELYAQACKYLPGGVNSPVRAFRSVGGHPLYFKLGKGS
                     HIIDEDGNEFIDYCMSWGPLILGHADNDVVKAIQQIAENGTSFGAPHAAEIDIARLVV
                     DSFPGMQKVRFVNSGTEATMSAIRLARGFTGNSKIIKFDGCYHGHADFLLVAAGSGLA
                     TFGMPDSAGVTKGNAKDTIVIPYNDVDALHQVLSHNDDIACCIIEPVCCNYGLILPED
                     DYLQKVRDLCTQYHVLLIFDEVITGFRLARGGAQERFGVTADITTLGKIAGGGLPVGV
                     YGASHEIMSKVAPDGPVYQAGTLSGNPIAMAAGIATLQKLVPSTYQKLEQLGQYFEDA
                     MQPVVKRFGGRFLFVRFASIFALYFTNADNITSVDMVKECNMQQFAQFHREMLERGIY
                     LSPSGYEVGFLSIAHTKEDIDTTVTAIDESLAVVMS"
     gene            complement(3210..4277)
                     /locus_tag="PLJ75_10185"
     CDS             complement(3210..4277)
                     /locus_tag="PLJ75_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HPD05621.1"
                     /translation="MLHSSSKLINLFDLVNDAIIIIDKNHNVIFNNEKGQIFVKSDGK
                     TLLLENETIINLVNTLQPDAEVIGEHISLKVKKRIHHYTLNAIAINDDNSFLIAFVIT
                     DTTRLHKLHQQQQQLISQIRKNYLDRFESLKQLADSIAHEVRNPLVSIGGYANLLIRK
                     CDEGIDHNEAKKYLQYIVDNAERLNYLTQQIEEMGDLKRVNLAAYSITKFMQEQESTF
                     IAAASQFNKKLQLNIESEKNYTIYIDYDKLRFAITQIIEFVAEHTKAENIVLHCYGTS
                     YELIIHLQCITDIIAEEDLHYLFDPFYTTRSNVETFNLCIAQRIIMLHGGIITSSLTR
                     KTLHFKIALPQDKRLTHDNGK"
     gene            complement(4325..5221)
                     /locus_tag="PLJ75_10190"
     CDS             complement(4325..5221)
                     /locus_tag="PLJ75_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05622.1"
                     /translation="MYYPSYLTLYHTGELKKRIDALQNILTCCTLCPHRCRIDRRIKT
                     GKCRTGVMAIVATYHAHFGEESCLVGSHGSGTIFFANCNLSCIFCQNWDISQMPNGKP
                     VSPEQLADVMIRLQQKGCHNINLVSPTHVAAQIVEALPYAIERGLTIPLVYNSGGYDA
                     VETLQLLDGIIDIYMPDFKYMDETVAKHLSGAPDYPHHATAAIREMHRQAGDLVIEHG
                     IAKRGLLIRHLVIPENSARSDLVFKEIAKLSIDTYINIMAQYHPAYRAREHPALGRYI
                     TLEEFNQALQWAKAAGLWRFDR"
     gene            complement(5516..6502)
                     /locus_tag="PLJ75_10195"
     CDS             complement(5516..6502)
                     /locus_tag="PLJ75_10195"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPD05623.1"
                     /translation="MLINNHSYTFFLAPMAEVSTPALRKVIKQFCGEVVLYSEMLASG
                     AINAKAPHNEPLVQRHGFDTPFFYQIVGNNPAIMANAASILQDYGCDGIDINMACSAP
                     HIVKAGCGAQLLVDMRHARDIVKSCRKAVKNLLTVKMRAGYHDIDMDYLKQFIVMLQD
                     EGVNAITLHPRAAKWGFSRSAKWDVIDEIVKHAAIPIIGNGDITTPQLAMQRLHQTRC
                     AGIMIGRAAVQQPWIFALCNALYNNNPLTVTIDHAALWIDVLTNIQTMLPQKLHKSRA
                     HRFCFYFAKNCTFGHELFTIIRKLSAIDDIIKTINDYYMRNPMDQNKHYRVP"
     gene            complement(6507..6821)
                     /locus_tag="PLJ75_10200"
     CDS             complement(6507..6821)
                     /locus_tag="PLJ75_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05624.1"
                     /translation="MKKIMKILVMILFIIIIIIGIVTITNWNDINNFRPMVSGAFSKF
                     MCSCLFVEQRSEEQCLQWSKLDLPVSSYSVDHNEKSVTASALGKTSKAHYTNDRFGCT
                     LQ"
     gene            complement(6826..8040)
                     /locus_tag="PLJ75_10205"
     CDS             complement(6826..8040)
                     /locus_tag="PLJ75_10205"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD05625.1"
                     /translation="MSNLNRHSIKGILVVCLIVLFTCITEADEQQGNVTGTSNFKKPA
                     TQRDYWPTKEWKVKKPEDVGMNSQDLKKMELYAFSRLQPESERKGIRTDSVIIVKNGY
                     IVYEKYAGEFTRNKVHITWSVSKSFTNALFGIAIKEGKLSLSDPAWKFYPSLNRGQHK
                     NITVDHLMRMSSGLYSNETYEASPLKSTVNAMLFTIGHTDMAAYAASQDLEANPGEKW
                     EYASPTPNLLMAMLKNTMSAREYEQYPWEKLFNVIGMKKVVWERDVAGTFVGSSYIFT
                     TSQDMARFGYLFLNDGIWEGKRLLPQGWVAYSTTIAPAYYTTKLSKSDMADPAYGALW
                     WLNRDIPEKNMKRAFPDAPDDMFIAMGHWGQYIFIIPSLDMVIVYTGDNRDDTFNINT
                     FLKLIIDSISKR"
     gene            8357..9016
                     /locus_tag="PLJ75_10210"
     CDS             8357..9016
                     /locus_tag="PLJ75_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002663840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4433 domain-containing protein"
                     /protein_id="HPD05626.1"
                     /translation="MKTKNLINIELFRMIHINNVPHILKYGITHRYSKNSNPDYENIG
                     DISLIDTRENKKINVTNGGSTIIKEIKLGNYIPFYFGVRMPMLYVIQHGGNFVEKPIP
                     PSDIVYIVCDLEKIIKLSQEYYFTDGHATDNLTTFYDSSKINEIKNILDWQAINAQFW
                     GGEENLTIKWGKQAEFLVKGDLPVTIIKRYICYNDEAKSRLMSFGILGENIVIDQSAY
                     Y"
     gene            9036..>9533
                     /locus_tag="PLJ75_10215"
     CDS             9036..>9533
                     /locus_tag="PLJ75_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014771256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPD05627.1"
                     /translation="MKYITGNILESKTQALVNTVNIVGVMGKGIALQFKKVFPENYKK
                     YADACKKGEITIGKLFITKENTLYGEKLIINFPTKKDWRKPSEYEYIEKGLDELIKII
                     EEYKITSIALPPLGAGSGGLVWSKVKTIIEEKLKNIDIEILVYEPTKDIKEKLKKERI
                     KLTDAR"
BASE COUNT         2913 a         1692 c         1933 g         2995 t
ORIGIN      
        1 taaagtactc ctcctgcatc atatcccgcc actcgataca gcggctcatg atgtgatagg
       61 tatgctcggg cagttgtagg cgcaatttcc gtgccatggg tattcctccg tgttgttata
      121 ataattgaag tttctactct ataaaacgcc ggagatgaaa aaaaatgaaa agaaatggtg
      181 cattttttta taaatttgta aaaaaatata atggggtcag agcatactgc tattctgtca
      241 ttctaaagga gcatcagcga ctgaagaatc tatatttttt atgcaaataa gagattcttc
      301 gccttcgttt caaaatgaca tcggttaaca ctatcataga gcagctgcca gtaatcgaat
      361 atttccaccc tttcgttcaa agggggggat tatggggggt tcacttcgta cagaatgaca
      421 aactataatt tttccaaagg gaaatatgtg atacatcttc atattttttc tcatactttc
      481 atttgcaaat cgacaatagt tttatcactt cgtaagggga tatttatgcg ctaaataccc
      541 ccttaccaac ccctaaagcg cacctcaaac ggcggtgtgc cttatttact gtatatagtg
      601 tgttttgacc ttcgtgctgc ggacgacatc ctgtccttgt cctcgcacga gccatatcat
      661 gctacggcat ccgtgcctcc actactttct tttaacctca tcaagacaat ataaaatggg
      721 atcagagcct atatgtctga gtactcagac aaaaaaatga tcacttcttt ttataaaaat
      781 aatgaaatta cataaaaata tactttacta aaatttttat tatccatact tattattgaa
      841 aatactttga ttatttatga atatattcaa ttataatgta gattagtgca ttactgagag
      901 cagatgtaat atcattaaat aaaataatta agaatgtgaa tgaaaggagg cacatgatgc
      961 atccctatta cagacctcgt cggctgcggg aaaatgaaat aatacgtgat ttaattgctg
     1021 aaacaaaacc tgattataac aaacttatta tgccctattt tgttatagaa ggggcgggta
     1081 ttacacaagc catagagtct atgccgggta tacagcggct atctattgat aatcttttaa
     1141 aacagcttga ttatgatgta aaattagggg tcagagccat tatcctgttt ggaataccgt
     1201 atgataaaga tgaaaaagcc tccggtgcgt atgatgaaaa tgggattgtc cagaaggctt
     1261 gcagggaaat taaaaaacag tttccacaat tgctcattat caccgatgtg tgtttgtgtg
     1321 aatacaccag tcatgggcat tgtggtatgg taaaaggaaa ccagatagat aacgatagta
     1381 cccttgagct tttagcaaaa actgccgtat cgcatgcaag ggctggtgct gatattattg
     1441 ctccttcgga tatgatggat ggcagggttg aaactattcg tcaggaactt gatgataacg
     1501 gttttataac tatacccata ctatcgtatg cagcaaaata tgcttcagct ttttatggtc
     1561 cgttcaggga tgcagcaggt agcgcaccac aatttggtga cagacgaagc tatcaaatgg
     1621 actatcgcaa tgcacatgaa gcattgaaag aggttaatct tgatattgac gagggtgctg
     1681 atatcgttat ggttaagcct gcgttatcat atttagatat tatttacagg gttaaacagt
     1741 ctgtagaatt gccggtgtgt gcatataatg ttagtggtga gtattccatg gtgaaagctg
     1801 caggtacact gggatttggc gatgaggcaa agcttgtgcg tgaaattatg acatcaatat
     1861 tcagagccgg agctgatatg gtaatttcgt atcatacctg tgatatttta aaaaacaggt
     1921 ggtttgatta ggaggattgc atggcattga ttacatcaaa atcaaaagaa ttatatgcac
     1981 aggcatgtaa atacctcccc ggaggtgtta attcaccggt gcgtgcattt agatcggtgg
     2041 gaggacatcc cctgtacttt aagttaggga agggctcgca catcattgat gaagatggca
     2101 atgaatttat tgattactgc atgtcatggg gtccgcttat tttaggtcat gctgacaatg
     2161 atgtggtaaa ggcaatccag caaatagccg aaaacggaac aagctttggt gctccccacg
     2221 cagcagagat tgacattgcc aggcttgttg tggattcgtt tccgggtatg caaaaggttc
     2281 gttttgttaa ttcaggcact gaggcaacca tgagtgcaat acgccttgca cggggattta
     2341 ccggtaacag taaaataata aaatttgatg gatgttacca cggtcatgct gattttcttc
     2401 tggttgctgc cggttcaggt cttgcaacat ttggtatgcc tgattcggca ggcgtaacaa
     2461 agggcaatgc aaaggataca atagttattc cctataatga cgtggatgcc cttcaccagg
     2521 tactatcgca caatgatgat attgcatgtt gcattataga gcctgtttgc tgcaattatg
     2581 gattaatttt gccggaagat gattatttac aaaaggtacg ggatctatgt acacaatatc
     2641 atgtactgct gatctttgat gaggttatta caggatttcg tctggcacgg ggtggcgctc
     2701 aggaacgctt tggtgttacg gctgatatta caacgctggg caagattgca ggtggcggtt
     2761 tgcctgtggg tgtgtatggc gcttcgcatg agataatgtc aaaggttgca cctgatggtc
     2821 cggtatatca ggcaggtaca ttatcaggca acccaatagc aatggctgct ggtatagcaa
     2881 cacttcaaaa gcttgttccg tctacatatc aaaaacttga gcagttaggt caatactttg
     2941 aggatgcaat gcaacctgtt gttaagcgct ttggcgggcg ttttcttttt gtcaggtttg
     3001 catcaatatt tgctttatac tttactaatg ccgacaacat taccagcgtt gatatggtaa
     3061 aggaatgcaa catgcagcag tttgctcaat ttcacaggga gatgctggag cgggggatat
     3121 acctttctcc atcagggtat gaagttgggt ttttatcgat agctcataca aaagaggaca
     3181 tcgatacaac agtaacagca attgatgaat cacttgccgt tgtcatgagt taaccgttta
     3241 tcctgaggca atgctatttt aaaatgaagg gttttacgag tcaggcttga ggtgataata
     3301 ccgccatgga gcatgatgat acgctgggca atgcagaggt taaatgtttc tacattggag
     3361 cgtgttgtat aaaaaggatc aaaaagatag tgcaggtctt cttcggctat tatatcggtt
     3421 atacattgca ggtgaatgat caactcatag gatgtcccat agcaatggag cactatattt
     3481 tctgcttttg tatgctcagc aacaaattca ataatctggg taatagcaaa gcgtaatttg
     3541 tcataatcaa tatatatggt ataatttttt tcggattcta tatttaattg taattttttg
     3601 ttaaactgac tggcagcagc tataaaggtt gactcctgtt cctgcataaa ttttgtaata
     3661 ctatatgctg ctaaattaac tcgctttaaa tctcccattt cctcaatctg ttgtgttaaa
     3721 tagttaaggc gttcagcatt gtctacaatg tattgtaaat attttttggc ttcattatga
     3781 tcaatgcctt catcacattt tcgtataagc aagttggcat acccgccaat agaaactaaa
     3841 gggttcctta cttcatgggc gatgctgtct gccagctgtt taagcgattc aaacctatct
     3901 aagtagtttt tacgtatctg actgatgagt tgctgctgtt gttggtgcaa tttatgcaaa
     3961 cgggtggtgt cggttataac aaatgcaatt aagaaagaat tgtcatcatt aattgcaatt
     4021 gcattgagcg tatagtgatg gattcttttt tttactttta aacttatgtg ttcaccaata
     4081 acttcagcat caggttgtag tgtattaacc aggttaatta tcgtttcatt ctctaaaagt
     4141 agagttttgc catctgattt aacaaaaatt tgtccttttt cattattaaa aataacattg
     4201 tgatttttat caataataat aatagcatca ttgaccagat cgaaaagatt aataagtttt
     4261 gacgatgaat gtagcataat gtttcattaa taattatgaa ttgtaatgaa gtcaatcata
     4321 aattctatcg gtcaaatctc cataaacctg cagctttagc ccattgcaat gcctgattaa
     4381 attcttccag tgttatgtat cttcccagtg cagggtgttc ccgtgctctg tatgcagggt
     4441 ggtactgagc cataatattt atataggtat caattgaaag ttttgcaatt tccttaaaca
     4501 ccagatccga gcgagcacta ttctcaggga tcaccaagtg ccttataagc aagccgcgct
     4561 ttgctatccc atgttctatc accaaatcgc cagcctgacg atgcatttcc ctgattgcag
     4621 cagttgcgtg gtgtggataa tcaggtgcac cgcttaaatg ttttgcaact gtttcatcca
     4681 tgtatttaaa atcgggcata tagatgtcta ttatgccgtc aagcaattgt aatgtttcca
     4741 ccgcatcgta tccgccactg ttgtatacca gcggaatagt aagaccgcgt tcaattgcat
     4801 acggcaatgc ttcaacaatc tgtgccgcaa catgtgttgg gctgacgagg tttatattgt
     4861 ggcatccctt ttgctgtaat cttatcataa catcggctaa ctgttcgggg ctaaccggtt
     4921 taccgttagg catctgcgat atatcccagt tttgacaaaa gatacacgaa aggttgcaat
     4981 tggcaaagaa gatggtaccg gagccatgac ttccaacaag gcatgattct tcaccaaaat
     5041 gagcatggta tgtggcaact atcgccataa caccggtacg gcatttacct gtttttatgc
     5101 gccggtcaat tctacatcga tgaggacaca gcgtacaaca ggtaagaata ttctgtaagg
     5161 catcgatcct ttttttaagc tcgccggtat gatagagtgt aagatatgac ggataataca
     5221 taactatcct tagtttgatc attccgtgaa tacgaaccat agattgctat agagcaaacc
     5281 ttgaaatgat cttttaaaaa atctttattt gcgtgtaata gtgaaagtat ttcttcaatg
     5341 tctttcatgg tttttttatg gtcacattca gcattatact atacaatcat aatttatatg
     5401 atactttcaa aacagcgata gtacaataaa aatgtataat actaatacat atcggaaatg
     5461 gcaaatattg tcaagaatat tgctttgtat ttataaaatg cacatgaggt atcctttacg
     5521 gaactctata gtgtttattt tgatccatgg ggttacgcat gtaataatca tttattgttt
     5581 taataatatc atctattgct gaaagcttgc ggattatagt aaaaagctca tgcccaaagg
     5641 tgcagttttt tgcaaagtaa aaacaaaatc ggtgtgccct gcttttatga agtttttgcg
     5701 ggagcattgt ttgtatgttg gtaagtacat ctatccagag agcagcatgg tcgatagtta
     5761 ctgttaatgg attgttattg taaagagcat tgcacagtgc aaaaatccac ggttgttgta
     5821 cggctgctct gccaatcata atgccggcgc accgtgtttg atgtaaccgc tgcatggcaa
     5881 gctgtggcgt tgtaatatca ccattgccaa ttatggggat tgcagcatgc ttaacaattt
     5941 catcaatgac atcccacttt gcgctacggc taaaacccca ctttgcagct cgtggatgca
     6001 gggtaatggc gttaacccct tcatcctgca gcatcacaat gaactgcttt aagtagtcca
     6061 tatctatatc atgatacccc gcacgcattt taaccgttaa aaggtttttt accgctttac
     6121 ggcagctttt aacaatatcc cttgcatggc gcatatctac aagcaattgc gcaccgcatc
     6181 cagccttaac gatatgggga gcactgcatg ccatattgat gtcgatgcca tcacagccat
     6241 aatcctgcag gatggaggcg gcatttgcca tgatagcagg attgttgcca actatttgat
     6301 aaaaaaaagg tgtgtcaaac ccatggcgtt gtaccagtgg ttcattgtgg ggtgcttttg
     6361 cattgatagc accgcttgca agcatctcag agtagagtac aacctcaccg caaaattgct
     6421 ttatgacttt gcgcagtgca ggtgtactca cctctgccat gggagcaagg aagaatgtat
     6481 agctatggtt gttgataagc atttagctat tgcaatgtgc atccaaaacg gtcattggta
     6541 tagtgcgctt tacttgtttt accaagagcc gaagccgtta cactcttttc attatggtca
     6601 accgaatatg atgaaacagg aaggtcaagt tttgaccatt gcagacattg ctcttcagac
     6661 ctttgctcaa caaataagca tgaacacatg aatttagaaa aagcaccgga aaccattggt
     6721 ctaaaattgt tgatatcatt ccaattggtt atcgtcacaa ttccaatgat gattattata
     6781 ataaacagta tcataacgag tattttcata atttttttca tggtatcacc ttttactaat
     6841 actatcgatt ataagtttta aaaaagtatt gatgttaaat gtatcatccc ggttatcacc
     6901 ggtatatact ataaccatat caagagaagg tataataaag atatactggc cccaatgtcc
     6961 catggctatg aacatatcgt caggagcatc gggaaatgct cgtttcatat ttttttccgg
     7021 aatatcacgg ttaagccacc ataaagcgcc atacgctgga tcagccatat cactttttga
     7081 aagtttggtt gtatagtatg ccggggcgat agttgttgaa tatgcaaccc atccctgggg
     7141 aagtaagcgt ttgccttccc atataccatc gtttagaaaa agatagccaa accgtgccat
     7201 atcctgcgaa gtggtgaaga tatatgaaga tccgacaaat gttcctgcca cgtcgcgttc
     7261 ccatacaact tttttcatac caataacgtt gaagagcttt tcccatggat attgctcata
     7321 ttctcttgca ctcatggtat ttttaagcat agccattaaa agatttgggg ttggactggc
     7381 atattcccat ttttcaccgg gatttgcttc caggtcctga gaagcggcat atgcagccat
     7441 atcggtatgt cctatggtaa acagcattgc atttaccgtt gatttaagcg gtgaagcttc
     7501 atacgtttca tttgaataca atccggagct catgcgcata aggtgatcaa cggtaatgtt
     7561 tttatgctgt ccgcgattta acgaaggata aaatttccat gcaggatcac tcaaggataa
     7621 ttttccttct tttattgcaa tgccaaaaag ggcattggta aatgattttg atactgacca
     7681 tgtaatgtgc accttgtttc tggtaaattc accggcatac ttttcatata ctatatagcc
     7741 attttttaca atgatgacgc tatcagttcg tatgcccttg cgttcagatt caggttgtaa
     7801 tcgtgaaaac gcatacaact ccattttttt taggtcctgt gagttcattc ccacatcttc
     7861 aggtttttta accttccatt cttttgttgg ccagtagtcc cgttgagtag ctggcttttt
     7921 aaaatttgat gttccagtaa cattaccctg ttgttcatct gcttctgtga tgcaggtaaa
     7981 tagtacaatt aaacaaacaa ccagaatacc tttgatactg tgtctattca aattgctcat
     8041 ggcacccccc attaattttt tattagtact acactattag tactacacta ttagtactac
     8101 actatcagta aaacagttat ttatgcaata cataaaaaat aatatttttg actgctattt
     8161 tgcttacaat attcacatgc gataatacag acagatatga ttttttacca cattcgtgtt
     8221 gacttttttc attgccgggt atagtattta tatttttaca ttgcaatgaa aaaggctgat
     8281 gtttatcata aaaaatccaa actatagatg gtatggtaaa gcataacaat gcctgagtgg
     8341 aattgaaagt gtgtgtatga aaacaaaaaa tttaatcaac atagaattat ttcgaatgat
     8401 acatattaat aatgtaccac acattctgaa atatggcatt actcatagat attcaaagaa
     8461 tagtaatccg gattatgaaa atattggaga tatttctttg attgacacaa gagaaaataa
     8521 aaaaatcaat gtcactaatg gaggcagcac aataatcaaa gaaataaagt taggcaatta
     8581 tatacctttt tattttggtg taaggatgcc tatgttatac gtaattcagc atggtggcaa
     8641 ctttgtagaa aaaccaatac caccgagtga tattgtctat attgtttgtg acttagaaaa
     8701 aatcataaaa ttgagtcaag agtattattt taccgatggt catgcaactg ataacctgac
     8761 cactttttat gatagtagca aaataaatga aataaaaaac atacttgact ggcaggctat
     8821 caatgctcaa ttctggggag gagaggaaaa tttgacaatt aaatggggca aacaagcaga
     8881 atttttggta aaaggtgatt tgcctgtaac catcatcaaa agatatattt gctataatga
     8941 tgaagctaaa agcaggttga tgagttttgg gatattaggg gaaaatattg taattgatca
     9001 aagtgcctat tattaataat agagaggggg gtatgatgaa atatattaca gggaacatcc
     9061 ttgaaagtaa aacacaggct cttgttaata ctgtaaatat tgttggagta atgggtaaag
     9121 gcattgcttt gcaatttaag aaagtatttc ctgaaaatta caagaaatat gcagacgcat
     9181 gcaaaaaagg tgaaataact ataggtaaat tattcattac taaagaaaat acgctttacg
     9241 gtgaaaaact tattataaat ttcccgacta aaaaggattg gagaaaaccc tcagaatatg
     9301 agtatattga aaaaggttta gatgaattaa ttaaaattat tgaagaatat aagatcacaa
     9361 gcattgcttt gccgcctctg ggcgctggca gtggtggatt ggtctggagt aaggttaaaa
     9421 ctattattga agagaagctg aagaatattg atattgaaat attggtatat gaaccgacaa
     9481 aagatattaa agaaaagtta aagaaagaaa gaataaagct aacagatgca aga
//
LOCUS       DAOOJH010000091         9516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6355_length_9516_cov_8.501216, whole genome shotgun sequence.
ACCESSION   DAOOJH010000091 DAOOJH010000000
VERSION     DAOOJH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_9516_cov_8.501216"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1184
                     /locus_tag="PLJ75_10220"
     CDS             114..1184
                     /locus_tag="PLJ75_10220"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPD05628.1"
                     /translation="MNSILTKIQEAINLLPATLMQLKQEGRKIIMTQCDILPYEVFRA
                     FGYSVIKMPRWVANAFIMGDSSLENDAVAFCTIADMIIVPKQCTIVPNAILSNRIFLI
                     DGINGFAEDAAVAIHSMLSMILRTVGTTQEYPDDEELKEAVCLYEEIRKIMRTVSSFH
                     AHKLSLIQLQLICDAAFSLLPDESLPLLQQIIVALESMDVPVPDNGEEKIKTLLYSDG
                     TNWEMFDECKKHDLTIVEDDACNVRRQFDISCNVSSTNLYYELLMAYTFKAWCPAMRS
                     APERFELIYKALPNYDIGLVIFLPSVLTIRNNHIDYLYEQCLLQGSNAIVCTEEEAIV
                     QIQRYITALKQRKHIAITIDLL"
     assembly_gap    1261..1360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1361..2964)
                     /locus_tag="PLJ75_10225"
     CDS             complement(<1361..2964)
                     /locus_tag="PLJ75_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD05629.1"
                     /translation="MKKLTYVCIALVLFSGCYKKSVVLNPNDIHYIISTFLNEHVQYH
                     EFNNTISDRTLNNLLSSIDPGKYYFYKNDVDGFTKRYSTLIDDLSKKEDFSFLDDIFG
                     TYRKRVAEVNDMINQLLKQQYTFTEDETINLDSDKISYAIDKKELKERWRKNIKLQLL
                     NYQTTDNLKEEQAKEKLKRKFFINNKRIQELDKAKQFSIFLNAFSMALDPHTNYLTQE
                     EHEDFMISNNLKLEGIGVQLRSEDGYTIVDRIIPGGAADKLSKELQLQPNDRIVAVAQ
                     DNDEPVDVVDMDLRDVVKMIRGKKGTLVKLTIIRINPTSNEQKRMVIPIVREEINLED
                     SAVKSEVHQSKNGMVIGYIKIPSFYVDITKQGIDQGRSSTADTIMQINNLMKKNVACI
                     VIDLRGNPGGALSEALKLAGLFVDAGPIMQVYDSSDTIQSLDDPIPGVYYSGPLVVLI
                     DKFSASASEIFAGAIRDYRRGIIIGTDATFGKGTVQSYKELYQKKGAIKITNAIFYQP
                     SGTSNQLNGIKPDITVPDITTIWDIG"
     gene            3165..4592
                     /gene="gatA"
                     /locus_tag="PLJ75_10230"
     CDS             3165..4592
                     /gene="gatA"
                     /locus_tag="PLJ75_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014547070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPD05630.1"
                     /translation="MIGKSMQELIALLESNAVTSVEIVQAFLQEMNHKQELNAYITVL
                     DDDALQQAQQSDKRRKAGKPLSQFDGIPIAIKDNICTQGILTTCASGILKNFIPPYNA
                     TVYQKLLNAGFIVLGKTNMDEFAMGSTTETSFFGPTKNPVDITKVPGGSSGGSAAAVK
                     ACCAPVALGSDTGGSIRQPASLCGVVGIKPTYGRVSRYGLVAFASSLDQIGTFANTVK
                     DAADVLAVISGVDRCDATSIAMPVDFGSDTITGTIKGLRIGVPREYFNGVDNQIVQAV
                     ETVIKQLELKGAIVHTISLQYTDYAVPIYYLIATAEASSNLGRYDGVRYGYRSPNIAK
                     LKDLYTKTRQEGFGKEVKRRIILGTFALSSGYYDAYYLKALKGRRLIIDDFNKAFEEC
                     DCIVAPVTTTTAFGIGEKIADPLALYMADILTISANLAGIPGMSVPVAEDTHGLPIGV
                     QLMARHFNEMVMLNVAQAIEDSIGR"
     gene            4570..5469
                     /locus_tag="PLJ75_10235"
     CDS             4570..5469
                     /locus_tag="PLJ75_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05631.1"
                     /translation="MKIALVVSIAVHCACAYRYTALRIGTVVTVPEHESVAPEQLTAT
                     VYYTAYVVTMTKGGDEAKKNNAAVEALLKGKIDDALVVLESIVAYNEPAVLNNYGVAL
                     LINGNSDSGLQYICRAAVLQPDNAYFKKNYLYLHECKNDIVIFFLAIVCCIGCLHEPV
                     DSDYYDDLSLVKGGEKPTPPVDLSLTVADHEVTLQFYTDRLDSGNPVTYDPDTGTNEN
                     LYYLVYIASNDPAMFGDPELYYDIRYYLGYVAEIDFAAGVDPKEVSIAIASSYHGPLF
                     VWMASHDGGRESDHSEVVFVGIP"
     gene            5568..6131
                     /locus_tag="PLJ75_10240"
     CDS             5568..6131
                     /locus_tag="PLJ75_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05632.1"
                     /translation="MNYRIAMVLFFIFYTPALCGQNKPFTFVPAEQPLSLYKALAYDI
                     VPGGGHIYTGHYEYAAVFATLKISGVYAMYYYYNYWHYRGSLYRSAKKANAAIDPEHD
                     LQFKDPEGGYKTVEEFRQAYDRAAHYFTLSVAANVAIYVTSWLMTLYHVQQINEERQP
                     AYIGFAAHVLLDADAYGTVYAGYTVKW"
     gene            complement(6237..6311)
                     /locus_tag="PLJ75_10245"
     tRNA            complement(6237..6311)
                     /locus_tag="PLJ75_10245"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6274..6276),aa:Asp,seq:gtc)
     gene            6499..>7001
                     /locus_tag="PLJ75_10250"
     CDS             6499..>7001
                     /locus_tag="PLJ75_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05633.1"
                     /translation="MSLTIFDDIYHEIRKRNTSGGFKAVPYSDEFIKYAAGYYGLSME
                     LIKNIIQILINSHKIFSIEIIAEDKVRDIAHVEGYVVCDLVILRKLKSFYQSELIIQY
                     ENEFHKRLMVHQVVKEIFPMLHSLNNTDIGKVANLAIMLEELERFMEKHYEEFTEEWK
                     AKRLAYE"
     assembly_gap    7002..7101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7102..7574
                     /locus_tag="PLJ75_10255"
     CDS             <7102..7574
                     /locus_tag="PLJ75_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05634.1"
                     /translation="HYEEFTEEWKAKRLAYEISLSNIDTQLERKVEKQKVAGRSSSPG
                     GLKDRRKKRVVDAPQYNEFIDKSKSYPLERIFQIYGIDFFLRVQLRNYKFDYLKELVG
                     TKKIKRKSDLLLLKNMLNAVLHNRNSDHNLAQYEDAIYELMQTVNHHLFVERDF"
     gene            7592..8248
                     /locus_tag="PLJ75_10260"
     CDS             7592..8248
                     /locus_tag="PLJ75_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPD05635.1"
                     /translation="MVQRIITLFMMLLMAGAIAYSDTTIVFDKPLENANVDIMKAFKN
                     RKSERSYTSQLISIHHLSTVLWSGYGINRQDGKHTVPTAYGKNYMRLYVIYDGKAYRY
                     LPEKHCLQFIQAEVTTAMASTQGWVGKSTCVIVIVADMDGFPFYAGLQEQQLKYSYAT
                     AGCIAQNIYIACTGLKLGTCLVAGVNVTGCKKAFKLHENELPLFIMPVGYPSAHQHSI
                     "
     gene            complement(8414..9340)
                     /locus_tag="PLJ75_10265"
     CDS             complement(8414..9340)
                     /locus_tag="PLJ75_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05636.1"
                     /translation="MDTTILKFIIFQLCIWLPIGAGSYLAQHTAYNVQQLSSRLVTIN
                     LVSLEAFIVLWTTWGAELSVHYIILPFAGIVMVIMGLAAGAVVSFLLGFNAIQKHTVI
                     ISSSLANHGFTMGGFLCYLILGEHGLALSFIFITYFMPYVILVIFSYARYAGTKQPFS
                     LRNYIIVPQNMPLFAILIAIILHMLNIKRPAINIPIDILLCISIVIYYFSMGIYFTFA
                     NIKNYCLLHLLIAIIKFCIIPLCMYLLLCHFPLPDDIQSVIRLQSFMPAAIYSVITAI
                     LFHLDKEMATSIFVVNTVAFSIIVLPALIVIF"
BASE COUNT         2968 a         1607 c         1943 g         2798 t
ORIGIN      
        1 gtatacatat aatctatcgc aagtaaaata ataaattgta aacaatattt ttattgaaaa
       61 attaatcatc atgttattgc tgaattaaaa aaggggtgat ggcatatatg gatatgaata
      121 gtatacttac aaaaatacag gaagctataa atctacttcc cgcaacatta atgcagttga
      181 aacaggaagg aaggaaaatt ataatgacac aatgcgatat cctcccctat gaggttttta
      241 gagcttttgg atatagtgtg attaaaatgc cgcgctgggt tgccaatgct tttattatgg
      301 gcgatagttc tctggaaaac gatgcagtag cattttgcac tattgctgat atgattattg
      361 tccccaaaca atgtaccatt gtccccaatg cgatattgag caatagaata tttttaatag
      421 atggtatcaa tggttttgcc gaagatgcag cagtagccat ccattccatg ctttcaatga
      481 ttttgcgtac tgtggggact acacaggaat acccggatga tgaagaatta aaagaggctg
      541 tttgcctata cgaagagata cggaagatta tgcgcaccgt atcgtcgttc catgcacata
      601 aactaagttt aattcaatta cagcttattt gcgatgcagc attttcgttg cttcccgatg
      661 aaagcttacc attattacag cagatcattg ttgctcttga atcgatggat gttccagtac
      721 ctgacaatgg agaagaaaag attaagacac tgctctatag tgatggcact aactgggaaa
      781 tgtttgatga atgtaaaaaa catgatttaa caatagttga ggatgatgcg tgcaatgtca
      841 ggaggcagtt tgatatttca tgtaatgtct cttcaaccaa tttgtactat gagctgctca
      901 tggcatatac cttcaaagca tggtgccccg caatgcgcag tgcaccagag cggtttgaat
      961 tgatatacaa agctttaccc aattatgaca ttgggctggt tatcttttta ccatcggtgc
     1021 ttaccatccg aaataaccac attgattatc tctatgagca atgcttgctg cagggaagca
     1081 atgcaatagt atgtactgaa gaagaagcca ttgtacagat acagcgatat ataaccgcac
     1141 taaagcaacg aaaacatatt gcaataacga ttgatttgct gtgatataag ctatcgtgac
     1201 acagagagcg tttgcagcac tataaactgt cgctattgta aaactttaat ataatccaca
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacctatat cccatattgt
     1381 tgtgatatcc ggtacggtga tgtcaggttt tatcccattg agttgattgg atgtccctga
     1441 tggttgataa aaaatggcat tggttatttt gattgcgcct ttcttctgat atagttcttt
     1501 atatgattgc actgtacctt taccaaaggt tgcgtcagta ccaataatta tcccgcgccg
     1561 gtaatcacgg atagcgcctg caaaaatttc tgatgcgctg gcactgaatt tatcaatgag
     1621 tactactaat ggtccactat agtatacacc gggtattgga tcatccagag attgtatagt
     1681 atcagaacta tcgtacacct gcataatagg tccggcatca acaaataaac cagctaattt
     1741 taatgcttca ctcaacgcac ctccgggatt gccacgaagg tcaataacga tgcaggcaac
     1801 attttttttc ataagattgt taatctgcat gatggtgtca gcagtggaac ttctaccctg
     1861 atcaattccc tgtttggtaa tatccacata aaatgatggt attttaatat atccaattac
     1921 catgccattt ttagactggt gcacctcgga tttgacggca ctatcttcta aattaatttc
     1981 ctcacgcact ataggaataa ccatgcgctt ttgctcattg ctggtagggt taatccgtat
     2041 tatagtaagt tttaccagcg tgcccttttt cccgcgaatc atcttcacaa catcgcgcag
     2101 atccatatcc acaacatcaa ccggttcatc attatcctga gcaacggcaa caatgcgatc
     2161 attgggctgt agctgtaatt ctttggaaag cttatcggca gcacccccgg ggattatacg
     2221 atcaacaatg gtatagccat cctcagaacg caactgcacg ccaatacctt caagttttag
     2281 attgttggat atcatgaaat cttcgtgttc ttcctgtgtt agatagttgg tatgaggatc
     2341 aagcgccata gaaaaggcat ttaaaaaaat tgagaattgt tttgccttat ccaattcctg
     2401 aatacgtttg ttattgataa aaaattttct tttcaatttt tcttttgcct gttcttcctt
     2461 cagattatca gtagtttgat aatttaaaag ttgcagcttg atatttttac gccatcgttc
     2521 ttttagttcc tttttgtcga ttgcatacga tattttatca ctgtctaaat tgatggtttc
     2581 atcctcggta aaggtgtatt gctgcttcag caattgatta atcatgtcat taacctcggc
     2641 aacgcgtttg cgataggtgc caaaaatgtc atcaagaaaa gaaaaatctt ctttttttga
     2701 aagatcgtca atgagtgtgg aatagcgctt tgtaaatcca tcaacatcat ttttataaaa
     2761 atagtatttt cccggatcaa tagatgacaa taaattgttc aatgtcctgt ccgagatggt
     2821 gttgttaaat tcatgatact gtacatgctc atttaaaaag gtactgatta tataatggat
     2881 atcgttcgga ttaagaacaa ctgacttttt atagcagcct gaaaacaaaa caagagctat
     2941 gcatacgtat gttagctttt tcataaacta tcctgtgtat gttattgtaa ttttttataa
     3001 tatggactat tatacaataa acatttatag aagtcaaata attatcacta aaggctttgt
     3061 aaactttttt agcccttagt tacacttatg tacaataaat taatttgaca ttattatcat
     3121 tatgacaaca atgaactgtg taaaaacttt acgggagcta tgcaatgatt ggtaaatcta
     3181 tgcaggaatt aatagcattg cttgaaagta atgcagtaac ctcagttgaa atagtacagg
     3241 catttttaca ggagatgaac cataagcagg agcttaatgc atatattact gtccttgatg
     3301 atgatgcatt acagcaggca cagcagtcag ataaacgtcg taaggcaggg aagcctcttt
     3361 cacaatttga tggcattccc atagctatta aagataatat atgcacacag ggtatattga
     3421 caacctgtgc atcaggaata ctaaaaaatt ttattccacc ctataatgcc acagtatatc
     3481 aaaagctttt gaatgcagga tttatagtat taggtaaaac aaacatggat gaatttgcca
     3541 tgggttcaac caccgagaca tcgttttttg gacctacaaa aaatcctgtt gatattacaa
     3601 aagttcccgg gggaagcagc ggtggttcgg ctgctgcggt aaaggcttgt tgtgcaccgg
     3661 ttgcgcttgg ttctgacaca ggtgggtcaa tacgacagcc tgcgtcattg tgcggagtgg
     3721 tggggataaa gccaacctat ggaagggtat cacggtatgg attggttgcc tttgcttcat
     3781 cgcttgacca gattggtaca tttgcaaaca ctgtaaagga tgctgcagat gtattagcgg
     3841 ttatttcggg tgttgatcgc tgtgatgcaa catcaattgc tatgcctgtt gattttggtt
     3901 cggataccat cactggcact attaaaggtt tgcgaatagg agtcccccgg gaatatttca
     3961 acggagtgga caaccagata gttcaggctg ttgaaacagt cattaaacaa ttggagctca
     4021 aaggagctat cgtacatacc atttcgttgc aatataccga ttatgcagta cccatatact
     4081 atctcattgc tactgcggaa gcatcgtcaa atttaggacg gtatgatggc gtgcgctatg
     4141 ggtaccgatc gcccaatatt gcaaagctga aggatctcta tactaaaaca cgccaggaag
     4201 gatttggcaa agaggttaaa cgacgcataa tactgggaac atttgccctt agttcaggat
     4261 attatgatgc ctattattta aaagcattaa aaggaaggcg tcttataatt gatgatttta
     4321 ataaagcatt tgaagagtgt gattgcattg ttgcgcctgt gacaacaaca acagcttttg
     4381 gtattggtga aaagatagct gatccattag cgttgtatat ggcagacatt ttaacaattt
     4441 cggctaatct ggctggcata ccgggtatgt cggtgcctgt tgcagaagat actcatggtt
     4501 tgccaatagg tgtacagctc atggcgcgtc attttaatga aatggttatg ctcaatgttg
     4561 cgcaggctat tgaagatagc attggtcgtt agcattgcag tacattgcgc gtgtgcttat
     4621 cgctatacag cattgcggat aggaaccgtg gtgacagttc ctgagcatga atcagtagca
     4681 ccagagcagt taacagcaac agtatattac acagcctatg ttgttactat gacaaaaggt
     4741 ggtgatgagg caaaaaaaaa taatgctgct gttgaagcac tcctgaaagg aaagatagat
     4801 gacgccctcg tagtattaga atcaattgtt gcatataatg aaccggcagt attgaacaat
     4861 tatggtgtgg cgctgcttat aaacggcaat agcgatagtg gattgcagta tatctgcagg
     4921 gcagcggtgc tgcagcctga taacgcatac tttaaaaaaa attatcttta tctccatgaa
     4981 tgtaaaaatg acatagtcat cttttttttg gcgatagttt gctgtattgg ctgtttgcat
     5041 gaaccggtgg acagcgatta ttacgatgac ctgtcgctgg tgaaaggagg agaaaagccg
     5101 acaccaccgg tggatttatc gttaactgtt gctgaccatg aagtaacatt gcaattttat
     5161 accgacaggc ttgatagtgg caaccctgta acctatgatc ccgatacagg aactaatgaa
     5221 aatctgtatt atttagtgta tattgcatca aacgaccctg ctatgtttgg tgatccagag
     5281 ctgtactacg atatacgcta ttatctgggg tatgttgcag aaattgattt tgctgcagga
     5341 gttgatccta aagaggtatc aatcgccatt gcatcgtcat atcatggacc gctgtttgta
     5401 tggatggcat cgcatgatgg cggcagggaa agcgatcaca gtgaggtagt gttcgtgggt
     5461 ataccataac atggttatag caacggtgca gagatttgct taaatgtttt tgttatggtg
     5521 tgatttactg tagcagcata ttttaaaaat gatagtggat gatatgcatg aactatcgca
     5581 tagcaatggt attatttttt attttttata caccggcact atgtgggcaa aataagccgt
     5641 ttacctttgt ccctgcggaa caaccgctaa gcctgtataa agcgctggcg tatgatattg
     5701 taccgggagg agggcatatc tataccgggc actatgaata tgctgctgtt tttgcaacct
     5761 tgaaaatttc tggtgtgtat gctatgtatt actattataa ctactggcac tatcgcggtt
     5821 cattataccg ctcggccaaa aaggcaaatg cagctattga ccctgagcat gatttgcagt
     5881 ttaaagatcc tgaaggcggt tataaaactg ttgaagagtt ccgacaagca tatgaccgtg
     5941 cagcgcatta ttttacgtta agtgtagcgg caaatgttgc catatatgta acatcatggc
     6001 tgatgacact gtatcatgtg caacagatta atgaagaaag gcaaccagca tatataggat
     6061 ttgccgctca tgtgctgctt gatgctgatg catatggtac ggtgtatgca ggatataccg
     6121 taaagtggta atgatgcact caatacagtg aagcatggaa ttgcaaaaat ataggtattg
     6181 ccaaaccgtg aagtattaaa aataaaaaaa ggcttttata aaaagccttt ttttatcggg
     6241 gtgtacgagg ctcgaactcg cgacctcctg cgtgacaggc aggcactcta accaggctga
     6301 gctaacaccc ctcaacgctt acaactatac tacaaatggt aaatggagtc aacttctgtc
     6361 aattaaaaaa atcacgtttt tatatatatt gcaaaaaata tttaaaaaaa gctttaaatt
     6421 tttttataac tattatatct gtaaaattgt ctgctactat taagaaggga tgatacgtgc
     6481 actgaaaggg agaaaagcat gtcattaaca atttttgatg atatatatca tgagatacga
     6541 aaacgaaata catcaggtgg tttcaaagct gtaccatatt cagatgagtt tattaaatat
     6601 gctgccgggt attatgggct ctcaatggag ctgataaaaa atattataca gatacttatt
     6661 aattcgcata agatattttc aattgagata atagcggaag acaaagtgcg tgatatagct
     6721 catgtagaag gttatgtagt atgcgattta gtaattctca gaaagttaaa atcattttat
     6781 caaagtgagc ttattattca atatgagaat gagttccata agcggttaat ggttcatcaa
     6841 gttgttaaag agatattccc catgctccac agccttaata atactgatat tggtaaagta
     6901 gcaaatctgg caataatgct tgaagaatta gagcggttta tggaaaaaca ctatgaagag
     6961 tttaccgagg agtggaaggc aaaacgttta gcatatgaga tnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn naacactatg aagagtttac cgaggagtgg aaggcaaaac
     7141 gtttagcata tgagatcagt ttatcaaata tagatacgca attagaacga aaagttgaaa
     7201 aacaaaaagt tgcaggcagg tcatcatcgc cgggtggact aaaagatcgt agaaaaaaaa
     7261 gggttgtcga tgctccgcaa tataatgaat ttattgataa aagtaaaagt tatcctctgg
     7321 agcggatttt tcagatttat ggaattgatt ttttcctgcg tgtgcagttg cgtaattata
     7381 aatttgatta tcttaaagag ctggtaggga caaagaaaat taaacgaaag tcagatctgc
     7441 tattgttaaa aaatatgctg aatgcggtac ttcataaccg gaatagtgat cacaatttag
     7501 ctcaatatga agatgccatc tatgaactta tgcagacagt taatcatcac ctttttgttg
     7561 aaagggattt ttaattttta agggaggttg tatggtgcaa cgtataatta cattatttat
     7621 gatgttattg atggcaggag ctatcgccta ttctgataca actattgtat ttgataagcc
     7681 tctggaaaat gcaaatgtag atattatgaa ggcatttaaa aacagaaagt ctgaacggtc
     7741 gtataccagt caattaattt caatacatca tctttcaact gttttatggt cagggtatgg
     7801 tataaacagg caggatggca aacacacagt gccaacagca tatggtaaaa attacatgag
     7861 gctatacgtt atctatgatg gcaaggcata tcgctattta ccggaaaaac attgtttgca
     7921 gtttattcag gctgaagtga caactgcaat ggcttccact caggggtggg ttggtaaaag
     7981 tacatgcgtt attgtcattg tggctgatat ggatggcttc cctttctatg ctggattaca
     8041 ggagcaacaa ttaaagtatt cgtatgcaac agccggctgc attgcacaaa atatatatat
     8101 agcctgtaca ggcttgaagt tagggacatg ccttgttgca ggtgttaatg tcacagggtg
     8161 taaaaaggca ttcaaattac atgaaaatga actaccacta tttatcatgc cagtaggtta
     8221 tccgtcagca caccagcaca gtatttagta tatatgggta acagaaattg aacgtagtta
     8281 cattggtgta cccctgcata aatgacaata cctcgttatc ccttattggt gaatactatc
     8341 tgcctatcat gaactcccat catggtacag ttttatattc aaggatagta gtgctgccaa
     8401 tgaacaaaga catttaaaaa atgacaataa gtgcaggcag tacaattatt gaaaatgcaa
     8461 cggtattaac gacgaatata ctggtagcca tctctttatc taagtgaaac agtattgcag
     8521 taataactga atatattgca gcaggcataa aagattgcag tcggataacc gattggatat
     8581 catctgggag aggaaaatga cacagcaata ggtacatgca aagagggatt atacaaaact
     8641 tgatgatggc aataagcaga tgaagtaagc aatagttttt tatattggca aatgtaaaat
     8701 aaatacccat gctaaagtaa taaataacga ttgaaataca cagtaatata tctataggga
     8761 tatttatagc aggcctttta atatttagca tatggagtat gatggcaata agtatggcaa
     8821 atagcggcat attttgaggt acaatgatgt agtttcgtaa agaaaatggt tgtttggtgc
     8881 cagcataccg cgcatacgaa aaaatgacca gtataacgta tggcataaaa taggttataa
     8941 agataaatga cagtgctaat ccatgttctc ccagaatcaa ataacataaa aatccaccca
     9001 tagtaaaccc atggttggct aacgaagaac taatgataac ggtatgcttt tgtatagcat
     9061 tgaaacccag aagaaaactt actacagcac ctgctgctaa tcccataatc accataacaa
     9121 tgccggcaaa gggcaggata atatagtgta cagaaagctc agcaccccat gttgtccaaa
     9181 gcacaataaa tgcttctaaa cttacaagat ttatagtaac aagcctgctc gatagttgct
     9241 gaacattata tgcagtatgc tgtgcaagat aggatcctgc tccaatgggt agccagatgc
     9301 acaactggaa gatgataaat ttaagtatag tagtatccat acgatttttt tcatgatagc
     9361 tatgtaacat aatgcctatg gtaagtgcat aaaagaatca tgattgtggt caattacatt
     9421 atgaaaaaaa tgttatgcga aacctctttc catccgatgt aatagatgag gtcagctaac
     9481 ctgttcaagg agggacaggt gaccaataaa aggggt
//
LOCUS       DAOOJH010000092         9490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6375_length_9490_cov_9.074616, whole genome shotgun sequence.
ACCESSION   DAOOJH010000092 DAOOJH010000000
VERSION     DAOOJH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_9490_cov_9.074616"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLJ75_10270"
     CDS             complement(<1..648)
                     /locus_tag="PLJ75_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05637.1"
                     /translation="MKNILVVATGIVILIGTLTPLKSNEPYIEIEDIYDTHGVLEEWL
                     YPGKYSPVNIFDNDINTIYAEGDDSYYMTIEIKFKNPVECDAIQVLGGVAVNKDIYEK
                     NNRPKDVVVYVYDTDESFLKDKALFEKEIQLKDVMEYQKITFEKTYSIKRFTFQGLGV
                     YKGSKYNDTCITELKFYNKGKEIPVNDVEKLKKEYVKWVGERLKKFFAIGKFRVEE"
     gene            complement(661..1824)
                     /locus_tag="PLJ75_10275"
     CDS             complement(661..1824)
                     /locus_tag="PLJ75_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05638.1"
                     /translation="MQGTLENAILAGADMLKGKGMHDALLYQNWEKTQYRAADWLSDW
                     AAQQGRQDAEDWKTNQKKEGILGLAWLGLSKAGSLLSGAWDSITGFFSNLFERQPQDT
                     MTALQRQKDIEQSGKMWKSGAMQKEVDKNIIAREAKYVKEIQDSYTNKDIRQSDDNDN
                     NIKEYNGNSKEMYERLMYYEAKLRTMGVDNLATLLQLGIPVVIGEEGKGAGLTCTDNK
                     GNTTGDTYNDRMSLIYTDGDGNIRIEEYNRANVDVSVLSGNGSVSDGGYDFIVGMHGK
                     NNDFKALNVFSFDNYDKLDTKDQKDILNKLKSNTLTYDKIKNSDILRLLPALGNKTQI
                     GINVHGGTNSYTDSKGCFTIYGMDWGSSTPQKDYKKFIDRFNFNQLGKIYIIR"
     gene            complement(2152..2829)
                     /locus_tag="PLJ75_10280"
     CDS             complement(2152..2829)
                     /locus_tag="PLJ75_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05639.1"
                     /translation="MMKAIKYITMFCLLFFITLVSANENDMRKIFDSIVYTNEPVASS
                     ELVEKGLPKDFYGAKKAFDGKVETAWVEGVEGDGIGEYIIFNLNFASIPGYDEFEDKN
                     KYIEVELTIVNGVAKNKKLYEMNNRVKKAILEVYEAEVAIRQIDPWVLSEQEPILNTT
                     FVLNLKDDMKPKKFLLRFRPKQKPIYGLSSSFLCIGKLIIKEVYPGTKYKDTGISEIQ
                     AKRVDDN"
     gene            complement(3232..3741)
                     /locus_tag="PLJ75_10285"
     CDS             complement(3232..3741)
                     /locus_tag="PLJ75_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05640.1"
                     /translation="MRGKLQKISIILIILLSIGCRLEKGNNMNKEEKVKKEQEECMLS
                     VEEQRNALKEFEGFCKELLSKAEDAKDLTELKEYFVSIPGFEKNEFCAMVRPVTVKDL
                     ITVLKNPDWVNKDNIFEKSVICISFIKVMSAKTIVDGDDGYFISLEFVYNGIKKRWEI
                     FNFEIMPNP"
     gene            complement(3767..6817)
                     /locus_tag="PLJ75_10290"
     CDS             complement(3767..6817)
                     /locus_tag="PLJ75_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05641.1"
                     /translation="MDRAVEKDLYGIWAGDSSGYQSARAIGAESPGMLRAMIAYMGYT
                     EGIPHEVINQLDAMIGTDNEAAVSELFARYVKQYEGINDRELAQRRALQVHQWQMTQQ
                     QMQDKKADWDRMVKRIFERGIRQWDMMLKGFASRWKNWQEESEESMVKGAKQWDETMQ
                     RLREEKLQWLADAYSGVSYKELLKRMAGIEVVVTDMLATIKEQYGDAIGKVDVHAILV
                     DIIKDQPQLLSSELMAMTKQQVEFGLTRVSVGSYNRSIFDDAQMLGKEYATVQKKTQN
                     IALLKALTQLLEQCREQIEAANEQAEQAALQFVSAYAFGIDGNRYKRTLAITSTRQTI
                     PAYQPFVYTDSLLLGEYSIPVLMDIYNKDDGVQFEATMNVVMAQAQTRMGMMMRPDVV
                     WGFSYYVGQFGQIKPGPKLKEGKGQYGRITKDVYEAEKEKATQDAICQYVNAGIGVAL
                     SAVTANPMVGAAYVSVGQAGDIAAGDLSLQHWAVQTGISMGASYAGGVVEESYGAVLG
                     SLTSSGIQGLGNFVEYKDNGGLGLQWGSKAQWRSWGIGSTTALVGAVLGEAYGEKWNN
                     SWQQYGYTASSSYVVNRFDGTGSWKDDMLQAGASVAALWASNALLADGTAIGQSASSH
                     NAVLPMNRFMQGTLENAILAGADMMKGKGMHDALLYQNWEKTQYRAADWLSDWAAQQG
                     RQDAEDWKTNQKKEGILGLAWLGLSKAGSLLSGAWDSVSEFFEKGINWVQGEGFYSNK
                     EIADNAAFAAFHKMAVKEAFQKRAQEILDSFYKDSREVTDAKKQALKEAGYDTTQLEA
                     EIARRRQALEEKKALTQHKEQKKNVVEIKETDYYDVDDYGDGKDWVKFKKEGEGFKDY
                     CLENQQDHKYVAREFAEIVSDAIDELHDKTGKTLYVNDFSPEPGKKSEFGHHDRDYDR
                     GYVVDIKWVSKDDKIYAGDYDNYKNLYDRESTLKLIESFIESSKTYLNNNGNRKMIIY
                     YNDTDVMENIKNKYIDMIKKNYLSIKYATGHSNHIHIGLYNRPK"
     gene            7195..8688
                     /locus_tag="PLJ75_10295"
     CDS             7195..8688
                     /locus_tag="PLJ75_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05642.1"
                     /translation="MKKQNVCTIGNLLLLTLMIACGGGGGGGGSSSVVPSIILPPSGI
                     NASDGSYNNCVVITWNAVSNAKTYVIYRSDVINGTYDKIGDEVSTSYEDSTVTKQKPY
                     FYRVSAISQTDAESEKSQADSGYSDDMPGAITVTATNGDNPDRVSVSWNSDANSTQYR
                     VYRSRAANGSYELIGTFQTTSCEDTTITPGLNYYYKVQGGTDYPTWGQLSNYDNGYAS
                     LQTPAASASQGTYTNRITITWADIPYETSYTVHRSTSQDGTYTQIATVATNSTSYNDT
                     SSTVINNPDGDYYYKVQAVSEVASQLSNASLGYIALPYPSGITATDGTCYQHVTISWN
                     AVTGATSYNVYRSETQDGMYSQIASSITATSYNDTTATETTHYYYKVSATKNRESQPG
                     NADEGYKKDPATSTLFRITWQANREKAVNSNGGGYRVYYNQTGTFAYGDSYFEIPYSS
                     GLLAPTHYDFERNSAQTGTWYVWVVAYSNLDGILLSLQSTPIQVTVE"
     gene            8714..>9490
                     /locus_tag="PLJ75_10300"
     CDS             8714..>9490
                     /locus_tag="PLJ75_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPD05643.1"
                     /translation="MKPGKTNILFTLALSIIITTSLCFSHAAFGKQKPLNSANTMKLN
                     TSTVKNSQMKGAFVQQYVPGEVIVKFKNTTNLQIKSQSIAAIGHTMLKNLGSTSIVHI
                     KLKENESVEHAIHAYKNNPNVEYAQPNYIYHIFSTPNDSSYGQLWGLNNTGQTITGGT
                     YPNNNPGTTGCDIDAESAWDKITDCSSVIVAVIDTGVNYNHEDLADNMWDGSASGYNY
                     HGYDFVNSDYDPMDFNGHGTHVAGIIGAVGNNGKGVTGVCW"
BASE COUNT         2575 a         2291 c         1399 g         3225 t
ORIGIN      
        1 ttcttctact ctgaatttgc ctattgcaaa aaactttttt aatcgttcac ctacccattt
       61 aacatactct tttttcagct tttctacatc gttaaccggt atttccttcc ctttattata
      121 aaactttaat tctgttatgc aggtatcatt gtatttactt cctttataaa cgcctaatcc
      181 ttgaaatgta aaacgtttga tagaatatgt tttttcaaaa gtaatttttt gatattccat
      241 gacatctttt aattgtattt ccttctcaaa gagagcttta tctttcaaaa atgattcatc
      301 agtatcatat acatatacaa ctacgtcttt aggcctgtta tttttttcat atatatcctt
      361 atttacagct actcctccca atacctgtat tgcatcacat tctactggat tcttaaattt
      421 aatttctatt gtcatgtagt agctgtcatc cccttctgca taaatcgtat ttatatcatt
      481 atcaaatatg ttcaccggtg aatatttccc cggatacagc cattcttcca gcaccccatg
      541 agtatcgtat atatcttcaa tttctatata cggttcatta ctctttagtg gtgttagtgt
      601 acctattagt attacaatac ctgttgctac caccaatata ttcttcatcg tttcacctca
      661 ttatcgtatt atataaattt ttcctaattg attaaaattg aagcggtcta taaacttctt
      721 gtaatctttc tgcggtgtgc tacttcccca atccattcca taaatggtaa aacagccttt
      781 tgaatcggta tagctgtttg ttccgccatg tacattaatt cctatctgcg ttttatttcc
      841 caatgcaggt aataatcgta aaatatcaga attttttatt ttatcatatg tcaatgtatt
      901 ggattttaat ttattcaaaa tatctttttg atctttagta tctaatttat cataattatc
      961 aaaactaaac acattgagtg ctttaaaatc attattttta ccatgcattc ctactataaa
     1021 atcatatccg ccatcggaaa cactgccatt accactgagg acactgacat ctacatttgc
     1081 acggttatat tcttctatcc gtatattgcc atcaccatct gtgtaaatga gtgacatcct
     1141 gtcattatac gtatcgcctg tcgtgttccc tttgttatca gtacaggtta gtcccgctcc
     1201 tttcccttct tcccctatta caactggtat ccctaattgt aacaatgttg ccaagttatc
     1261 cacccccatt gtcctgagct ttgcctcata atacattaag cgctcataca tttctttact
     1321 gtttccgtta tattccttaa tgttattgtc attatcatct gattggcgta tatctttatt
     1381 ggtataggaa tcctgtattt cttttacata ttttgcttcc cgtgctatga tatttttatc
     1441 aacctccttc tgcatcgcac cacttttcca catctttcct gattgttcta tatctttttg
     1501 ccgttgtaat gctgtcatag tatcctgtgg ctgtctttca aatagattgc tgaaaaagcc
     1561 tgtaatgcta tcccatgcac ctgacagcag gctgccggct ttacttaatc ctaaccatgc
     1621 cagtcccagt atcccttctt tcttttgatt ggtcttccag tcttcggcat cctggcgccc
     1681 ctgttgcgct gcccagtcgc ttaaccagtc cgctgccctg tactgtgtct tctcccagtt
     1741 ttgatataat aacgcatcgt gcataccttt acctttcagc atgtccgctc ctgccagtat
     1801 ggcattttcc agtgtgccct gcataaacct atctgctatt tcccttcgta atgggatatt
     1861 tatacgctca ctatcccctt accaacccct caagcgcacc tcacacggcg gtgtgcctta
     1921 ccccctgtat gtagtatgtc tttgccttcg tggctatgtc ctcgcacgag ccgtatggcg
     1981 ctctggcatc ctgcctccgc tgttttgctt tcaaatgaaa aaactttatg caccagccat
     2041 ttccatagtg ttcgtgtgat actttagcaa aaaactgttg ccttaatatc tttttcttgt
     2101 atcccaaaaa cactatacat tagtgctaaa cctgcttgta gcagtatttt tttaattatc
     2161 atcaaccctt tttgcttgta tctcacttat ccccgtatct ttatatttcg ttcccggata
     2221 cacttccttt attatcaatt tgccgataca caaaaaacta ctacttaatc catatatagg
     2281 tttttgtttt ggtctgaatc ttagtaaaaa cttttttggt ttcatatcat cttttaaatt
     2341 aagtacaaat gtcgtattca atatcggttc ttgttcagat agtacccatg gatctatctg
     2401 ccgtatagct acttctgctt catatacttc taatatcgcc ttttttaccc tattattcat
     2461 ttcatatagc ttcttgtttt ttgctacacc attcactatt gttaattcaa cttctatata
     2521 tttattttta tcttcaaatt catcataccc aggtatagaa gcaaaattca aattaaatat
     2581 gatatactca ccaattccat caccttcaac tccttctacc catgctgttt caactttccc
     2641 atcaaatgct ttctttgctc cataaaagtc ttttggcaac cctttctcga ctaattctga
     2701 acttgctaca ggctcattag tataaacaat actatcaaat attttacgca tatcattttc
     2761 attggcacta accaatgtaa tgaaaaacaa taaacaaaac atagtgatat attttatagc
     2821 tttcatcata gtctttctcc tcaatcaata attattcttt tatcatttga tataggcatc
     2881 cattttttac ctatatcata cctgccatac ttatacttct ttaccacatc gtatgtgctc
     2941 gcgcacatcc atattgcgcc gtacctccct gtacggctgc tgttaatttt ctttcgtcat
     3001 agataatata gctgtcttca gagatcagtt gaatacagtt agtaaaaaaa atttttaatt
     3061 cgaacattta taatgtagca caattaacaa actccctaac tccaaattac acgggtacgc
     3121 tctatccttt ttttaacata aatgactttt acacattaat agtctaaatt gatatctact
     3181 cattttttta agattttact aacaaaaatt cagataatat tactatacca tttaaggatt
     3241 aggcataatt tcaaaattaa aaatttccca tctttttttt ataccattat aaacaaattc
     3301 taaacttata aaataaccat catcaccatc cacaattgtc ttggcagaca taacctttat
     3361 aaatgatatg cagattacac ttttctcaaa tatattgtct ttgttgaccc aatctggatt
     3421 tttcaaaacc gtaattaaat ccttcaccgt tactggtctt accattgcgc aaaattcatt
     3481 tttttcaaac ccaggaatac ttacaaaata ttcttttagc tctgttaaat cttttgcatc
     3541 ttcagctttt gataataatt ctttacaaaa accttcaaac tccttcaatg catttctttg
     3601 ttcctctaca cttaacatac attcttcttg ttccttcttt actttttctt ctttattcat
     3661 attattcccc ttttctaatc tacatccaat tgacaatagt attatcagga taatactaat
     3721 tttttgtaat tttcctctca taatcattca ctccttttat tatttttcac tttggcctat
     3781 tatatagacc aatgtgaata tgattactat gcccagttgc atactttatg ctcaaataat
     3841 tttttttaat catatctatg tatttatttt ttatattttc cattacatct gtatcattat
     3901 aataaattat catttttcga tttccattat tatttaaata tgttttcgaa ctttctataa
     3961 acgattctat taactttaat gtactttccc ggtcatatag attcttgtaa ttatcataat
     4021 cacctgcata aattttatca tcctttgaaa cccattttat atccactaca tatcccctat
     4081 cataatccct gtcatgatgc ccaaattctg acttcttccc tggctcagga ctaaagtcat
     4141 ttacatatag agttttacct gttttatcgt gtaattcatc tatagcatca gatactatct
     4201 cagcaaactc cctcgcaaca tatttatgat cttgctgatt ttctaaacaa taatccttaa
     4261 atccttcccc ttctttctta aatttaaccc aatccttacc atcaccataa tcatctacat
     4321 cataataatc cgtttccttt atttcaacta cattcttctt ctgttcttta tgctgtgtaa
     4381 gtgccttctt ctcctctaat gcctgcctgc gtcttgctat ttctgcctct aactgtgttg
     4441 tgtcataacc tgcttccttt agtgcctgtt tctttgcgtc tgttacctcc ctgctgtctt
     4501 tgtaaaaact atcaagaatt tcttgtgctc gcttctgaaa ggcttctttt acagccattt
     4561 tatgaaatgc cgcaaacgct gcattatccg ctatctcctt attgctataa aatccttcac
     4621 cctgtaccca gtttatccct ttttcaaaaa actcactgac gctatcccat gcacctgaca
     4681 gcaggctgcc ggctttactt aatcctaacc atgccagtcc cagtatccct tctttctttt
     4741 gattggtctt ccagtcttcg gcatcctggc gcccctgttg cgctgcccag tcgcttaacc
     4801 agtccgctgc cctgtactgt gtcttctccc agttttgata taataacgca tcgtgcatac
     4861 ctttaccttt catcatgtcc gctcctgcca gtatggcatt ttccagtgtg ccctgcataa
     4921 acctattcat gggcaatacc gcattgtgac tgcttgcgct ttgtcctatt gctgtgccgt
     4981 cagccaatag tgcattgctt gcccataacg ctgctacact tgcccctgcc tgcagcatgt
     5041 catctttcca gcttcctgta ccatcaaaac ggtttactac atagctgctt gatgcagtat
     5101 acccatactg ctgccatgag ttgttccact tttcaccata ggcttcacct aacactgctc
     5161 ccaccagcgc cgttgtactg cctattcccc agcttcgcca ctgtgcttta ctcccccact
     5221 gcagtccaag cccgccgtta tccttgtact ccacaaagtt acccagtccc tgtatgccac
     5281 tgctggtaag cgaccctaat actgctccat agctctcctc taccactcct cctgcatagc
     5341 ttgctcccat acttatccct gtttgtaccg cccagtgttg caggcttaaa tctccggctg
     5401 ctatatcccc tgcctgtcca acactcacgt atgctgctcc taccatgggg tttgctgtta
     5461 cagcactgag cgccacgcct attcctgcat tcacatactg gcatattgca tcctgggttg
     5521 ccttctcctt ctctgcctca tacacatcct ttgttatgcg cccatactgc cctttccctt
     5581 cttttagctt tggcccgggt tttatctgcc caaactgccc tacatagtag ctaaatcccc
     5641 ataccacatc aggccgcatc atcatcccca tccgtgtctg tgcctgtgcc atcaccacgt
     5701 tcatggttgc ctcaaactgc accccatcat ctttattgta tatatccatc aacaccggta
     5761 tactgtattc ccctaacagc aaactatcgg tatatacaaa gggctgatat gctggtattg
     5821 tctgccttgt gctggttatg gcaagcgttc gcttgtacct attgccatct atgccaaacg
     5881 cataggcgct tacaaactgc aacgctgcct gctctgcctg ttcattggct gcttctatct
     5941 gttctctgca ttgctccagt aactgcgtta gcgctttcag taacgctata ttttgtgtct
     6001 tcttctgtac tgtagcatat tcctttccca gcatttgcgc atcatcaaag atactcctgt
     6061 tatagctccc aacgcttacc cgtgtcaagc caaattctac ctgttgcttt gtcattgcca
     6121 tgagctcact acttaaaagc tgcggttgat cctttattat atccacaagg atggcatgaa
     6181 catcaacctt gccaatggca tcgccatact gctcttttat tgttgcaagc atatctgtta
     6241 ctactacctc tatccctgcc atgcgcttta atagctcctt atagcttacc ccgctatagg
     6301 catcagccag ccactgcagc ttttcctcac gtaaccgctg cattgtctca tcccactgct
     6361 ttgccccctt caccatggat tcttctgact cttcctgcca gttcttccac ctgctggcaa
     6421 atcccttgag catcatgtcc cactgccgta tcccccgctc aaatatccgt tttaccatcc
     6481 tgtcccagtc ggctttctta tcctgcatct gctgttgtgt catctgccac tggtgtacct
     6541 gcaatgccct ccgctgcgct aattctctat cgtttatccc ctcatactgt ttcacatacc
     6601 gggcaaacag ctcgcttacc gctgcttcat tgtcggtgcc tatcatcgca tctaactggt
     6661 ttatgacctc atggggaatc ccttcggtat aacccatata ggctatcata gcacgcagca
     6721 tccctggcga ctcagcccca atggcacgtg ctgattgata cccggaacta tcgcccgccc
     6781 atattccgta taaatccttt tctaccgccc tatccaccac taccctatca taccccccgc
     6841 ttgtatacag cgtttgggtg tgcgctatgt atatttataa attttttctt gacaaaatat
     6901 gctatccatt acaataattt tgttgattag gattagtttt ttcgttactg atgatgtcat
     6961 cagaataagt aacgagtaaa taaaacaaaa aaattataaa acgaatactg tgtgaaggag
     7021 ttgatatata catataaatg tatgggacat catctcataa taaataatat cccaatttta
     7081 ttttagaagg ctcataatcg tggattaata atatcaatcc attttgcatt gttcatatat
     7141 acatttaatt ttaaatataa gaatacaaat aagaatatga tagggaggag ttttatgaaa
     7201 aaacaaaatg tttgtacaat tggtaatcta ttgcttctaa cattgatgat tgcatgcggt
     7261 ggtggaggcg gcgggggcgg ttcaagttca gtcgttccat caatcattct gcctccatcg
     7321 ggcattaatg caagtgatgg ctcttataac aactgtgtcg ttataacatg gaatgcagta
     7381 agcaatgcta agacatatgt catctatcgc tctgacgtca tcaatggcac ttatgacaaa
     7441 attggcgatg aagtatcaac cagttatgaa gattcaactg ttacaaaaca aaaaccatac
     7501 ttctacaggg tttcagctat ctcgcaaact gatgctgaaa gtgaaaaaag tcaggcagat
     7561 agtggctatt cagatgacat gccaggtgct attactgtta ccgctaccaa tggcgataac
     7621 cctgacaggg tcagtgtatc atggaattct gatgccaatt caacccagta tagagtgtat
     7681 cgttcacgag ccgccaatgg cagttatgaa ttgattggta ctttccaaac aacatcatgc
     7741 gaggatacta caataacgcc aggattaaac tattattata aggtacaggg cgggaccgat
     7801 tatccaacat gggggcaatt gagtaactat gacaatggat atgccagttt acaaactcct
     7861 gctgcttcgg cttcacaggg aacatacacc aacaggatta ccataacatg ggctgatatt
     7921 ccatacgaaa catcatatac tgtccaccgt tcaacaagtc aggatggaac ctacacacag
     7981 atagcaacag ttgcaaccaa tagcacatca tataacgata ccagttcaac cgtaataaat
     8041 aatcccgatg gagactatta ttataaagta caggctgttt ctgaagtagc aagccagctt
     8101 agcaacgcat cattggggta tattgcgctt ccctatccat cagggataac agcaaccgat
     8161 ggcacctgct atcaacatgt tacaatttca tggaacgctg ttaccggcgc cacatcatac
     8221 aatgtgtaca ggtctgaaac ccaggatgga atgtattctc aaattgcttc atcgataaca
     8281 gctacaagtt ataatgatac aactgccaca gaaaccacac attactatta caaggtcagt
     8341 gctacaaaaa atagagagag ccagcctggc aatgccgatg aaggttacaa aaaagatcct
     8401 gcaaccagta cactattcag gataacatgg caggctaata gggaaaaagc tgttaacagc
     8461 aatggaggag gatatagagt atattacaat caaacaggta catttgccta cggcgatagt
     8521 tactttgaaa ttccatattc cagtggttta ttagcaccca ctcactatga ttttgaacga
     8581 aactcagcac aaacaggtac ctggtacgta tgggttgttg cctactcaaa ccttgatggt
     8641 attttgctaa gtctgcaatc aacacctata caggttaccg ttgaataata ctatgaacaa
     8701 aggagttttg attatgaaac caggaaaaac aaatatacta tttacattag cattatccat
     8761 tattattacc accagcttat gtttcagtca tgcagccttt ggtaaacaaa agccattaaa
     8821 tagtgcaaat actatgaaac ttaatacaag caccgtaaaa aactcacaga tgaaaggtgc
     8881 atttgttcaa cagtatgtcc ccggtgaggt gatagtaaaa tttaaaaata caaccaattt
     8941 acagataaaa tcacagagca tagccgccat tggtcataca atgctcaaaa atttaggttc
     9001 aacatctatt gtgcatatta aactaaaaga aaatgaatca gttgaacatg ctatccatgc
     9061 atacaaaaat aaccccaatg tcgaatacgc ccagccaaat tatatttacc atatcttttc
     9121 aactcccaat gattccagct acggtcagtt atggggacta aacaataccg ggcaaacaat
     9181 aaccggagga acatatccca acaacaaccc tggtacaaca gggtgtgata tcgatgccga
     9241 atcagcatgg gataaaataa ctgattgctc ttcggtgata gtagcagtaa tagacacggg
     9301 cgttaactat aaccatgagg atttagcaga caatatgtgg gacggttccg cttccggata
     9361 taattatcat ggctatgact ttgttaatag cgattatgat cccatggatt ttaatgggca
     9421 tggtacccat gtagcaggca ttataggagc ggtagggaat aacggaaaag gcgtcaccgg
     9481 tgtatgctgg
//
LOCUS       DAOOJH010000093         9473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6388_length_9473_cov_10.303992, whole genome shotgun sequence.
ACCESSION   DAOOJH010000093 DAOOJH010000000
VERSION     DAOOJH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6388_length_9473_cov_10.303992"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLJ75_10305"
     CDS             complement(<1..1025)
                     /locus_tag="PLJ75_10305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPD05644.1"
                     /translation="MQILANILIIFSIAIAVIYICNRFKIPTIIGLLLTGLLVGPYGF
                     QFVKTPDEVELLSEIGIITLLFTIGLELSLEEITSLKRAVVAGVLQVLVTIVIVAVIM
                     LAWNSFSRSVFFGFIVSLSSTAIVLKILQEYALGDSPHGRISLAILIVQDIAIVPLML
                     FIPLLSGDVNHSITTEMLLLLLKGGVVIVLVLVLARYVIPKFLFEIAKTRSRELFIIS
                     VVTICLAITVATYSLGLSIELGAFLAGVIIAASEYSGQTFSSMIPFRDLFTSFFFISI
                     GMLLDPSIVMLNIITMSSLSLGVIAIKLFIITAIVIIVGYPLRTALLTGFALAQIGEF
                     SFILAQIG"
     gene            complement(1077..1544)
                     /locus_tag="PLJ75_10310"
     CDS             complement(1077..1544)
                     /locus_tag="PLJ75_10310"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD05645.1"
                     /translation="MYEFREALLSDIDDVVTIRMKMLFELGKLETEDGTRKLRSLTRD
                     FFIRKFQTGEFKVFCAVTDETIIATTGIQFLDRPPLYENPHGIEAYIMNVYTEPDYRG
                     KGVASKLLDTVINYAKERKAGRILLHAIGKDKRIYEKKGFTSTTDEMQLILTY"
     gene            complement(1635..2048)
                     /locus_tag="PLJ75_10315"
     CDS             complement(1635..2048)
                     /locus_tag="PLJ75_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05646.1"
                     /translation="MIELSHNNYSSVLQSKLYNVTKHPYHFASFIAIWYYVELLYMMS
                     IAVFMYPPMLIAIVAIVTGIALSFHIIKLYSGNDFSCSLQLLLMDIHIAFSIGTIATI
                     LLSKVEWYILLFTLLRSIVAICEVVCVYAMTNDKS"
     gene            2127..3083
                     /locus_tag="PLJ75_10320"
     CDS             2127..3083
                     /locus_tag="PLJ75_10320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD05647.1"
                     /translation="MDDKKTITLTVPDEYSNTRIDVFIFNALEGKLSRSFIQKLLKGG
                     NITVNGNSVKQNYKVKHQQEITIAIPKPEKTSLQPQDIPLTILFEDNDIAVIYKPAGM
                     VVHPGAGNVEHTLVNALLYHFKGLSSIGGVERPGIVHRLDKDTEGLMIIARNDTAHTI
                     ASKAFQQRTVIKKYEAIVIGKPNEVSGLINKAIDRHPKYGHKMAVRDDGKEALTQYTC
                     KEIWHTANGVFSRLELQIHTGRTHQIRVHLASIGLPIVGDQLYSKKWEKYRVPYMLLA
                     ATHLQFEHPITKQPLQFYVDLPIHIKDFITRLNTLAIEHIIP"
     gene            3090..3518
                     /gene="tsaE"
                     /locus_tag="PLJ75_10325"
     CDS             3090..3518
                     /gene="tsaE"
                     /locus_tag="PLJ75_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPD05648.1"
                     /translation="MKQVHYTTSSQEETFAVGYELGKKAKKGDIFCLIGDLGTGKTIL
                     AKGIAKGLGINEEITSPTFTLLEVYEAAIPLYHFDLYRISDDSELENLFFEEYWYGDG
                     VSVIEWAERAMKRLSNDIFIIRLSYTGKNRRKITIEHPDP"
     gene            3499..4191
                     /gene="tsaB"
                     /locus_tag="PLJ75_10330"
     CDS             3499..4191
                     /gene="tsaB"
                     /locus_tag="PLJ75_10330"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPD05649.1"
                     /translation="MNILILDTATSIEIVAIRYEGTIADKSSTTQNSHSVTLFSSIDD
                     ACKELGITIHDINCIAVGIGPGSFTGIRIAVTTARMLAQILEVPLVGIHTQMLYACSI
                     VTDNVDCIVAFDAKKSRVFAARYRIQQGKLPQVVIPPGDYPVRHLCEIDTSKHIIAIG
                     DGALKYNNEMTALIKNITFLPGFLPQPEAMVAMIENLYTDNPQKYADYHQVVPFYSRK
                     SDAEIMRELKNK"
     gene            complement(4206..5384)
                     /locus_tag="PLJ75_10335"
     CDS             complement(4206..5384)
                     /locus_tag="PLJ75_10335"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HPD05650.1"
                     /translation="MKEYKQRKSRNFENVATLDDVMELLKDEQFQKKLMIKFTLDKTS
                     IEINEFIDNRTIMLVTDPDYVPVQNKIILYGLADRYLEIECDVIEVAGPGYFKCKIVS
                     ARKAAHGRRDLRFKLNIEKVVATNFRVSKHTIDIRNYSIPTGIKVIVEQFENQISKNA
                     DIVKVDILDDRDPLLAHIRKTKSTFYIEDLNDPSTFAPVNDAFININDVFQDQTAQFV
                     KKLTDSGYKSIIISPVIYIENDERLIPFAFIQYISKENTFSMDKVLEVQDLAYKLVDR
                     IRDANTLMIAVHQEIVDISRGGAKLRITDSNLKKYILKSKGFIFDIVFKLQAPITIYG
                     DIRYTYLDNDDNVIVGVDFAGNSSRKDEMKRFYSIIQPMENEYRNRLIKEMRQKKKDQ
                     "
     gene            complement(5540..5740)
                     /locus_tag="PLJ75_10340"
     CDS             complement(5540..5740)
                     /locus_tag="PLJ75_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05651.1"
                     /translation="MEQKKSLKQLQAEEVRKKDKVKKLNLELNALKKDLQALAKSILS
                     AKKLEAEKKKAAPKSKKPAKKK"
     gene            6110..7414
                     /gene="der"
                     /locus_tag="PLJ75_10345"
     CDS             6110..7414
                     /gene="der"
                     /locus_tag="PLJ75_10345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPD05652.1"
                     /translation="MKDIPVVSIIGRQNVGKSTLFNAIIKQRKAIVDEQPGLTRDVVS
                     YFVNYNEATFIINDTPGLDLNANSTLSEEIKQMALQHLHSTDVFVLLLEKPDIASYDF
                     ELVELIRKFNKPYIVAINKIDTNNDMECLSNFYELAQEFLPISALNRRNIDLLLHTIV
                     ESLPHKRKKIIEIDCKIALVGRPNSGKSTLLNAFLGYQRAVVSDIPGTTRDSIDEIFR
                     FQGKNIMIIDTAGIRRQSRIDNSIEYYAIVRAREAIQKSDVVIHLLDGTSGITETDKK
                     IADIIMEAHKPTILAINKWDSIPKDHTTFESFKDRMIFKYYRAADFPIISISALHKLR
                     IHKLLLTALDVNNRAHHKTETSAINVIIENIQKKHAMPLLGGDLKIYYATQTNISPPV
                     IRLFVNKPELFRKDVYRFLEKTLQDELNWQGIPVVFEVSGRK"
     gene            complement(7416..8012)
                     /gene="recR"
                     /locus_tag="PLJ75_10350"
     CDS             complement(7416..8012)
                     /gene="recR"
                     /locus_tag="PLJ75_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPD05653.1"
                     /translation="MNLPSKYLEQCIHELSKLPGIGVKSASRIAFYLLKVPKPNVDTL
                     LTTIAQLKNNMQYCKQCGGISDADICTICSDNERDRTLLCVVEEPEDMLSIEKSGAFN
                     GLYHVLGGVISPLDGVGPEELSINKLVDRCKQGVNEVIIATNPTIEGDATALYIASLL
                     KPMNIKVMRIAHGLPVGSDLEFVDAPTLAKSITGRREM"
     gene            complement(8014..8343)
                     /locus_tag="PLJ75_10355"
     CDS             complement(8014..8343)
                     /locus_tag="PLJ75_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPD05654.1"
                     /translation="MNFKDVGQLMKAQSELKKIQKRLKKNEITASSRDEMVKATVNGD
                     YELVSIAIDEEKCKGSSIKTLERNITEAVNKAIADAKEFSSQEMMKISGEMDIFGMAN
                     ELKKFIK"
     gene            complement(8358..>9473)
                     /locus_tag="PLJ75_10360"
     CDS             complement(8358..>9473)
                     /locus_tag="PLJ75_10360"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05655.1"
                     /translation="ETILSRCQKFIFKRIPVASIVAQLLKIAEKEGYAIDNKSLFHIA
                     RAGSGSMRDAQSLLDQVISFGGNEITEQDVLLFLGIVSLQQYCDCIHIILKKETALLI
                     DFVESIISSGVDIARFVAGFADVVYAMRLSKLADISPILGYSDEETALLTKTAESLHD
                     EQLSLFYTMLLDCQNQLRFTGNERAVVEMFLIDIMKMLQRPTLATILQQLDTASPEPV
                     QKKNIENHVPKKATIENKDESKEALDKEPADDDTKTIMHAWTQMLHDWRNARQYLFFK
                     LTNVRTAYVNGQLVLSVMGDSSNGFYKQLLSKEEIKLIEQELYNRSKRNVSIKIKSGE
                     DDTPPPDAVMLPHAEDPEKPHPLAEQIKTMFDGEIIK"
BASE COUNT         3063 a         1860 c         1577 g         2973 t
ORIGIN      
        1 ccaatttgag ccaatataaa tgaaaattca cctatttgtg ctaatgcaaa gcccgtcaat
       61 aacgcagttc gcaaaggata tccaacaata ataacaattg ctgtaattat aaacaatttg
      121 atagcaataa cacccagtga taatgatgac atggtgataa tattcaacat aacaatggaa
      181 ggatcaagga gcatcccaat agatatgaag aaaaagctgg taaataaatc tctgaatggt
      241 atcattgaac tgaatgtttg cccgctatat tctgatgctg caatgatgac acctgcaagg
      301 aatgcgccta actcaatgga taaccccagt gaatacgttg ccacagttat tgccagacaa
      361 atagtaacaa cacttataat aaaaagttct ctgctacgcg tttttgctat ttcaaataaa
      421 aattttggta tgacatatcg tgccagtaca agtaccagca caatgacaac gccgcctttt
      481 aaaagcaata aaagcatttc agtagtaatt gaatggttca catcaccact caataaagga
      541 ataaataaca tcaatggaac aattgcaata tcctgaacta ttaatatcgc cagtgatatt
      601 cttccatgag gactatcgcc aagagcatat tcctgtaaaa tttttaaaac aattgctgta
      661 ctgctcagtg aaactataaa tccaaaaaaa acagatcgtg aaaaactatt ccatgcaagc
      721 attatcacgg caacaatgac tatcgtcaca agcacctgta aaacaccagc aactactgct
      781 cgttttaagg atgttatttc ctcaagcgat aattcaagac caatggtaaa aagtaaggta
      841 atgataccta tctcggataa cagttcaacc tcatcaggtg ttttgacaaa ctggaaacca
      901 taaggaccta ccagcaaacc tgtcagcaac aagccaataa ttgttgggat tttaaaacgg
      961 ttacatatat atataacagc tattgctatt gaaaaaataa tgagtatgtt tgcaagaatt
     1021 tgcataggct atgcaactca ggattgaatt aaagtaatgt tgaacaatcg aaataattaa
     1081 tatgttaaaa tcaactgcat ctcatcggta gttgatgtaa agcctttttt ttcataaata
     1141 cgtttatctt tgccaattgc atgaagcaat atccggcctg cttttcgttc ttttgcataa
     1201 ttgatgactg tatccagcaa tttagatgct acacccttac ctctataatc cggttcagta
     1261 tacacattca taatatacgc ttcaatacca tgtggatttt catataatgg agggcgatct
     1321 aaaaactgaa tacccgttgt agcaataatt gtttcatctg tcacagcaca aaaaacctta
     1381 aattctcctg tctgaaattt gcgaataaaa aaatccctgg taagtgagcg aagcttgcgc
     1441 gttccatctt cagtttccaa tttacccaat tcaaaaagca tcttcatcct tatagtaaca
     1501 acatcgtcaa tatcacttaa cagtgcttca cgaaattcat acatatatac cccctcatga
     1561 acaactaaac cttttagcaa aatccattaa tgcgtattgt atttgtttcg tcaaataata
     1621 aagttgtatc agtatcatga tttatcattg gtcatagcat aaacacaaac tacttcgcat
     1681 atagctacaa ttgagcgcag taaggtaaaa agcagtatat accattccac ttttgaaaga
     1741 agtatggtag ctatcgttcc tattgaaaat gcaatatgaa tgtccatcaa gagcaattgc
     1801 aaagagcaac taaaatcatt gccactataa agctttataa tatggaagct aagcgcaata
     1861 ccagtaacta tcgccacaat tgcaattaac attggcggat acataaaaac tgctatactc
     1921 atcatataca aaagctcaac ataataccag atagcaataa aacttgcaaa atgataggga
     1981 tgttttgtta cattgtacag cttgctttgc aatacgctac tataattgtt gtgcgatagt
     2041 tctatcacta ttgtcctcag aaaaaaatga tttgaccgtt tgtttaacat tattattaca
     2101 atgctttagt atttcaaggc ttttttatgg atgataaaaa aactattacg cttaccgtcc
     2161 ccgatgaata tagcaataca cgtattgacg tgttcatatt caacgcgctt gaggggaaac
     2221 tttcgcgcag ttttatacaa aagctcttaa aaggtggcaa cattaccgtc aatggaaatt
     2281 ctgtaaaaca aaattataaa gttaaacacc aacaggaaat taccatagct atacccaaac
     2341 ctgaaaaaac atcattacaa cctcaggaca tccccctgac cattctattt gaagataacg
     2401 atattgcggt catttacaaa ccagcaggca tggtggtaca tcccggagct ggcaatgttg
     2461 agcatacgct ggtgaatgct cttctttacc atttcaaagg attatcatcc attggcggtg
     2521 ttgaacggcc tggcattgtt caccgccttg ataaagatac tgagggtttg atgattattg
     2581 ccaggaatga tactgcccat actattgcat caaaagcatt tcagcagcga acggttataa
     2641 aaaaatatga ggcgatagtt atcggtaaac ccaatgaagt atcggggctt atcaataaag
     2701 caattgatcg ccatccaaaa tatggacata aaatggctgt gcgtgacgat ggcaaagaag
     2761 cgctgacaca gtatacctgt aaggagatat ggcataccgc caatggtgtt ttttcgcgat
     2821 tagaattgca aatccatact ggacgcaccc atcagatacg ggtacattta gcttcaattg
     2881 gacttcccat agttggtgac cagctttatt ctaaaaagtg ggaaaaatac agggtgccct
     2941 atatgctgct tgctgctacc cacctgcagt ttgaacaccc cattaccaaa caacccttgc
     3001 aattttatgt tgacttgccc atacatatca aagattttat taccaggctc aatacacttg
     3061 cgatagagca tataataccg taaaatagta tgaaacaggt acactatact accagctcac
     3121 aggaagaaac atttgctgtt ggctatgaat taggcaaaaa agcaaaaaaa ggtgatattt
     3181 tttgcttaat tggcgatctg ggaacgggaa aaactatact tgcaaaggga atagcgaaag
     3241 ggcttggcat aaatgaagaa atcaccagtc caacatttac cctgcttgaa gtgtatgaag
     3301 ctgcaatacc gttgtatcat tttgaccttt atagaatatc tgatgattca gagctggaaa
     3361 atttattttt tgaagaatac tggtacggtg atggtgtatc cgtcattgaa tgggcagaac
     3421 gagctatgaa aagactctcc aacgatatat tcatcatcag gctttcatat actggcaaaa
     3481 acaggaggaa gattaccatt gaacatcctg atccttgata cagcaacaag cattgagatt
     3541 gtagctatac ggtatgaagg cacaatagct gataaaagca gtacaactca aaattcacat
     3601 tcagtaacat tattttcatc catagatgat gcatgcaaag agcttggcat aactattcat
     3661 gacattaatt gcattgcggt agggattgga ccgggttcat tcaccggcat ccgcattgcc
     3721 gttactactg cccgcatgct tgcacaaatt ctggaagtac cgctggttgg tattcataca
     3781 cagatgttgt atgcctgttc gatagttact gataatgtgg attgtattgt agcatttgat
     3841 gcaaagaaat cacgggtgtt tgcagctcgc taccgtatac aacagggcaa attgccccag
     3901 gtggtaatac cccctggcga ctatcctgta cgccatcttt gcgagattga taccagcaaa
     3961 cacatcattg ccattggtga tggtgcgctg aaatataaca atgaaatgac agcgttaata
     4021 aaaaatatta cttttttacc cggtttttta cctcaacctg aagctatggt tgccatgata
     4081 gaaaatcttt atactgacaa tcctcaaaaa tatgctgatt accatcaggt tgtgccattt
     4141 tattcacgaa aatccgatgc agaaataatg agggaattaa aaaataagta ggctttatac
     4201 tattgttact gatctttttt cttttgacgc atttctttaa tgagacggtt tctgtattca
     4261 ttctccatgg gctgaattat tgaataaaac cgcttcatct cgtccttccg ggaggaattt
     4321 ccggcaaaat ccacgccgac aatgacatta tcatcattat caaggtaggt atagcggata
     4381 tctccatata ttgtgatggg tgcctgcagt ttaaatacaa tgtcaaatat aaaccctttt
     4441 gatttcagta tatacttctt caagttacta tcggtaatcc ttaattttgc cccaccgcgg
     4501 ctgatatcta ctatctcctg atgtactgca atcatcaatg tgtttgcatc tctaatccta
     4561 tcaacaagct tataggctaa atcctgaacc tcaagtacct tatccataga aaatgtattt
     4621 tcttttgata tatattgtat aaatgcaaat ggaatgagac gttcatcgtt ctctatgtaa
     4681 ataaccggag atataataat tgatttataa ccactgtcag ttaatttttt tacaaattgt
     4741 gctgtttgat cctgaaaaac atcatttata ttaataaacg catcattgac tggagcaaag
     4801 gtagatggat cattaagatc ttctatataa aaggttgatt ttgtttttct gatatgtgca
     4861 agcaatgggt ccctgtcatc cagtatatca acctttacta tatcagcatt ttttgaaatt
     4921 tgattttcaa attgttcaac gataactttt atacctgtag gaatactata attacgaata
     4981 tcaatagtat gtttggaaac cctgaaattg gtggcaacaa ccttctcaat atttaatttg
     5041 aagcgaagat cacgtctacc atgcgctgcc ttgcgtgcac tcacaatttt acatttaaaa
     5101 tatcccggtc ctgctacctc tataacatca cattcaatct ctaaatacct atcggcaagc
     5161 ccatacaaaa taattttatt ttgtacagga acataatctg gatcagtaac aagcattatt
     5221 gttctattat ctataaattc attaatttca attgatgttt tatcaagggt aaatttaatc
     5281 attaattttt tctggaattg ttcatctttc aacaactcca taacatcatc taaggttgct
     5341 acattctcaa aatttcgaga ttttctttgt ttatattcct tcaaattacc ctcaatacac
     5401 agaacaattg gtttatgcta tattttttat cgaaaatttt atatatttac ttcaccataa
     5461 aacattaaaa ttttttataa acaaagaggt ggataaccac ctctttaata atattaatga
     5521 ttaggtaata tcaaaaaact tatttcttct tagcaggctt ctttgatttt ggtgcagctt
     5581 ttttcttttc tgcttccagt ttttttgcag ataaaatact ttttgctaat gcttgtaagt
     5641 cttttttcaa agcattaagc tcaagattta actttttaac cttgtctttc tttcgtactt
     5701 cttcagcctg aagctgtttt aatgactttt tttgttccat cgattacacc acctcgattt
     5761 aaaatttttt acatgcatgg cgcatgatta aaaagtaaac aattaagcct gatcatgatc
     5821 atataacata tatattttaa tattgcaaca aaaaaatgat tttgatatat gaaaatatta
     5881 attttttttt aacatcattt tttataaata ttacttttat tttattaatg tatcaatcga
     5941 tctatattca tgaaaacatt agaaattaat ttccatatca ataaatatct attgacacca
     6001 tacatatatt catcagtagt agaaacaatc tgtagtactg tcgctactat caataattat
     6061 gcttgaaatc attatgattg gcaacaatca ttatagtaag agggatactg tgaaggatat
     6121 acctgtcgtt tcaataattg ggcggcaaaa tgttgggaaa tcaacccttt ttaatgcaat
     6181 tatcaaacaa cgcaaggcaa ttgttgatga acagcccgga cttacccgtg atgttgtttc
     6241 atattttgtg aactataatg aagcaacatt catcatcaat gacaccccgg ggcttgatct
     6301 aaatgccaat agtacactat ccgaggaaat taagcagatg gcattgcaac atctccattc
     6361 tacggatgta tttgtgttat tactggaaaa accggatatc gcttcttatg attttgaact
     6421 ggtagaactc atccgtaaat ttaataaacc atatattgtt gccattaata agatagatac
     6481 caataacgat atggaatgtc tctctaattt ttacgaattg gctcaggaat ttctgcccat
     6541 ttctgcatta aaccgccgca acattgatct actccttcac actatagttg aatctctgcc
     6601 gcataagagg aaaaaaatca ttgaaattga ttgtaaaata gctcttgtag gacgtccaaa
     6661 ctcagggaaa tcaacattat taaatgcatt tttaggatat cagcgtgcag tggtatcaga
     6721 cattcccggt accacacgtg atagcattga cgaaattttc agatttcaag gtaaaaacat
     6781 catgattatt gatacagcgg ggatacgacg acaaagccgt attgataatt caatagaata
     6841 ctacgccatt gtccgtgcca gagaagctat tcagaaaagt gatgtagtaa tccatctttt
     6901 agatggaaca agtggtataa ccgaaactga taaaaaaata gctgatatta tcatggaagc
     6961 acacaaacca acaatacttg caattaacaa atgggatagt ataccaaaag atcatacaac
     7021 ctttgaatca ttcaaggata gaatgatttt caaatactat cgcgcagctg atttccctat
     7081 tatttccatt tcagcattgc ataagcttag aatacataaa ttgcttttaa cagctttaga
     7141 tgtcaacaac agagcacacc ataaaactga aacgtctgca ataaatgtaa ttatagaaaa
     7201 tattcagaaa aagcatgcca tgccactcct tggtggagat cttaagatat actatgcaac
     7261 gcaaaccaat atttccccac cggttatacg gctttttgtc aacaaaccag aactcttccg
     7321 aaaagatgta tatagatttt tagaaaaaac attgcaggat gaacttaact ggcagggaat
     7381 tcctgttgtt tttgaggtaa gcggtagaaa ataggttaca tctcccttcg tccggtgatg
     7441 gattttgcaa gtgtgggtgc gtcaacaaat tctaaatcag agccaaccgg caagccatgc
     7501 gcaatacgca taaccttgat gttcatcggt ttcagaaggg atgcaatata cagcgctgtt
     7561 gcatctcctt ctattgttgg attggtggca ataataacct cgttcacacc ctgtttgcat
     7621 ctgtcaacaa gcttgttgat tgaaagctct tccggcccaa caccgtcaag aggcgaaata
     7681 actccaccca gtacatggta caaaccatta aaggcacccg atttttcaat actcagcata
     7741 tcctcaggtt cttcaaccac acataaaagt gttctatcac gctcattgtc actgcatatg
     7801 gtgcagatat cagcatcaga tatgcctcca cactgtttac agtattgcat attatttttt
     7861 aactgggcga tagttgtcag caatgtatca acattaggtt taggaacttt cagtaaatag
     7921 aatgcaatgc gcgacgcact cttcacgcca atacccggta attttgacag ctcatggata
     7981 cattgttcta aatattttga aggcagattc atgctacttt ataaactttt taagttcatt
     8041 tgccatacca aaaatatcca tctcgcctga tatcttcatc atctcttgag atgaaaattc
     8101 ttttgcatca gcaattgctt tattcaccgc ttcagtgata tttcgttcca atgttttaat
     8161 actgctgcct ttacattttt cttcatcaat agcaatggaa accagttcat agtcaccatt
     8221 gaccgttgcc ttcaccattt catcgcgtga tgaagcggta atttcgtttt ttttaagcct
     8281 tttctgaatt ttttttaact cgctctgtgc tttcatcaat tgtccaacat ctttaaaatt
     8341 catagtgttt acctctacta ttttataatc tcaccatcaa acatggtttt aatctgttct
     8401 gctaatggat gcggcttttc aggatcttca gcatgcggca gcatcactgc gtcaggaggc
     8461 ggcgtatcgt cttctccaga tttgattttt attgatacgt ttcgtttact gcggttataa
     8521 agctcctgtt caataagttt tatttcttct tttgatagca actgtttgta aaatccgttg
     8581 gaactatcgc ccataaccga aagtaccaat tgtccattta catacgctgt tctgacattg
     8641 gtcagtttaa aaaataggta ctgccgtgca ttgcgccagt cgtgcagcat ctgagtccat
     8701 gcatgcatta tggtctttgt atcatcatcg gcaggttctt tatccagtgc ttctttactt
     8761 tcatccttat tttcaattgt agctttcttc ggtacatgat tctctatatt ttttttttgc
     8821 accggctcag ggcttgcggt atccagttgc tgcagtattg tggcaagcgt tggacgctgt
     8881 aacatcttca tgatatcaat caaaaacatc tcaaccactg ccctttcatt ccctgtaaag
     8941 cgtaactgat tttgacagtc caataacatg gtgtaaaaca ggcttaactg ctcgtcatgc
     9001 aggctttcag cagtttttgt taacagtgcc gtttcctcat cagaatatcc taatattggg
     9061 gagatatcag caagtttgga taatcgcata gcatatacga catcagcaaa ccctgcaaca
     9121 aaccgtgcaa tatcaacacc tgaagaaatg atgctttcta caaaatcaat gagcagagca
     9181 gtttcttttt ttagaataat atggatgcaa tcacaatact gctgcagtga aacaattcct
     9241 aaaaacaaca acacatcctg ctcggtaatt tcgttaccac caaatgagat aacctgatca
     9301 agaagcgact gagcatcacg cattgaaccg ctacctgcac gcgctatgtg aaataacgac
     9361 ttattgtcaa tagcatagcc ttctttctca gctattttta aaagctgtgc aacaatgctt
     9421 gcaacaggga tgcgtttaaa aataaacttt tgacagcgcg ataaaatggt ttc
//
LOCUS       DAOOJH010000094         9212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6593_length_9212_cov_8.369553, whole genome shotgun sequence.
ACCESSION   DAOOJH010000094 DAOOJH010000000
VERSION     DAOOJH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6593_length_9212_cov_8.369553"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            917..7693
                     /locus_tag="PLJ75_10365"
     CDS             917..7693
                     /locus_tag="PLJ75_10365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HPD05656.1"
                     /translation="MDNSTVTLNLAYYTKKLHDLTKKSWYYCAKSVYKNRRLLAGNNP
                     VKTIFKSKSILIAIYNKCKKENFTHSQFTPLDFAFIQIFIDTYSRDVLEKVFLHSIAV
                     ELSQGQYAICTSTFCDDNFTLDDIVHFNKTASITILCHSNETAKSWPKNNICLEWYFR
                     LLEPLSNYTAFTRWLAKLFYNNTIYQNAQGIITSYDTLVNTFATYNRTTAADVIPLLS
                     KNNFWKFPDSDELYELVFDCEVAVKSTDIQQVGALLCCRGLVISTIDSLSQLKKLDAI
                     CMLRVIGYNILDFDLDILSHNNFSFSNPYEIVDLLWVYNILYPINDGSSLDDAFRRLV
                     GMSIEKRHTAIADSYAAWDVWCKIKELTSFHKGHFLKHLTNVTAIAHDKKAKRHCGDS
                     LRKFIASHTKGIIECNDTTDIIQQLIAATPDDQFAFIFKTAKDMRNTVAQLLLDKIIT
                     IDDISNSVFLDSEEFHIDSDKLKQVKPSHDVLQLYIQSLLLYNVSTTSLISLPLFIAE
                     HKQGSLMHQLQTIMESCICHGSPDIKSNAKYVFLNIGGFNTMKLSKTIFASRHVVFNS
                     IHQYDNLFTRLLSKQADIELTSDIINKLGLEDSNKVYITRDYIIHNTAYNNYEQFLHE
                     LLPLIHTSDGKWATTLFTRINTLNKFEYVPCIGKAGNTWKLIFEPLYWKADIDTLFSE
                     ASSMHFLAHFVDKSNEGALCYKIMFGIDDDAIFEKKDKLVPVKLKAYRSNLKQTKFNT
                     AVYRKNALDIIHNLAGTDAIKGKIVIICTNDEERDFYYNYCTRYFNTTIFRTDTIPPR
                     FFEKNITYHSNYIVITTIYDVNSIKGGNIDYVIVPRLSLGYDAPSINQWRKQHIEELL
                     LNENIEMLQLFVKHFGSAEADKVLGAFIYTYPFLNAMNVLYTMLLNNTIDTVAVTLMD
                     TSSLLLPGNQRMHKKFEEIFKSNDLVEESMVHVENLVKNELVDEIMGKEYLQLNKDLY
                     TMLLHKHWGKDKTFKIFNKDIKAFPDKEYIISNSGVIRQDDIIHYVMRSFHDPNCKDK
                     DSLVIAATGIGKSLIYQIPAIILSQELEPELTIVISPLIALMKDQVDGLHKHGVYSVA
                     QFNSTLESESRSVIKNLILEGFINIIYVSPEMIVQSKNFQDMIGRRPPSFLVVDEAHS
                     LSQWGHDFRIDYHIVASTIKQIFKYRDFPVAAFTATAKISKFENLSVVHDIIKIMEMD
                     ISRDRALLCTTIRDNLYYHVKTFESGTKLENYNLDEQKVTFLVNFFKNPARYIANEHK
                     QSILKDTSTDKKYKTIIYCSSVNLTNTLSEYLTSNTNYTFKPFNAKLEQYQKEDIQNR
                     FKAGDGIDGICATTAFGMGVDVPDIRIVIHYDLPQGIEAYYQESGRAGRDGKNSYCLL
                     LYQKKLITKNTSNKLENDYQRVQYLMGRDRIRLYDFSTIYNGIAVFLQQQPDKSKDCY
                     VNGTMLHALFIDQNNDASNKVDSLLFYMSDYYHGDMKGYWCYSKEKASLTYKILKPKI
                     HYSFTSDSTLNKVIWSVHKCLCRYKEQQSPESKSYVEITHQHLNNMLKKDGNYHFSQN
                     DLYRALSTLITHEVIRIDDVKSYYLNDKKAVGGLTALFDKMIKVCENYSVSTKQDGYG
                     TTCRIDFRKMLTGDHNKTINMNTLELIVSLIVKQYIDGKYNKNTRNKKNKNNEFVTIT
                     MKYPALNFILHRDAGEDKLIEAIDGLKYTFKKYRDEIIGIIEFLASPVCATSKRCSIY
                     ELWTRYEEDCVNHGKKSVNTYTFRYNIGLLQILGILDSSNNNDFEPSYTVKVYDINKS
                     PLEHINTAWEEIETIYNTKKASMKLLVQQYILDGLSTKDGKDGKDGKDDKDVFDKRRN
                     FFEEFFLKDSYDKMLLIQNKDFGEQACEYNNCFLSIAGTTANTFKKQDGKYTIEDVIA
                     YIASEVKKLQKQLIQQYVVSIKKPDDVPIITLKSVMYTEFMLLLLESIRIPVHKLSDC
                     VTITKTSEHRDAINNYEAFDRQQHKAYTVASFILYMLVISSYFTGFETFDNKDYIEYM
                     QKNNGTRHLDGGVTMDSDKFCKEVVEEAAGYVKKELKNTGYKNITYQEIAHYAEQFFT
                     TRYVLFNNYKKVPTSPSTNYSYYSNEALSKTIDNYIFKQQCIFRVFDVYNRFQNQNAV
                     EKHLASFEIERFLGNYLANAIQYYVNNNNNIYPQNILPMINHFAIVVVHDIENKWVNI
                     IKLFNPHAVVIKINDAIFNGDSKDDNAVYQYKKCEVLYEESYVYNCGDRQRTYRYSTG
                     NSGKYTNPKP"
     gene            7599..8840
                     /locus_tag="PLJ75_10370"
     CDS             7599..8840
                     /locus_tag="PLJ75_10370"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HPD05657.1"
                     /translation="MKKVMFITVGTGRERTDIAQAIVESIRIQSPDFVVFYCTHKSSN
                     ETMPEIYALNNALQVKSTVEIISDENNLEKIKKEISHSMAQYKKNYALNTVDYTSGTK
                     AMSAGIIVAAIENSCDYISYITGTRDNQGRVVTGTEQINTFRLNQIYATKYVELGKQF
                     FNNYLFGAAIDLFEKAKNICHDETLNNEVELYISLAQALNAWDKFDHEQAKNIFTAIT
                     KNKQAVVLLESKEIEKTIEIMKRHLHAIASSQFSDLLIIDLLKNAERRFAEGKYDDAM
                     ARLYRLLEFIAQVLLDKQNIYQKDAHGKSDTSKLDVEKLPQHLKEKYTPAIGLSKSYE
                     LLSDLNDETGKKIYDIMQKKDVKQILGIRNGSILAHGFVPVCRDITQKCIEIIKEIAS
                     IYFTLQQFEKLYHELEFITLE"
     gene            8845..>9212
                     /locus_tag="PLJ75_10375"
     CDS             8845..>9212
                     /locus_tag="PLJ75_10375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPD05658.1"
                     /translation="MADIYLISDYGKLYKQNKVFHFLYPDGTVAKFFPHNTQRMYVIG
                     NIQITAEALKMISHNKIEVIFLNKNGFFNGKLTFGDSKNVLLRKKQYEKLNDNDFILK
                     WGPAGLKEFSEGTDAVASYGS"
BASE COUNT         3307 a         1455 c         1690 g         2760 t
ORIGIN      
        1 aagcctgctg aagaatatgc aagataagga ttgtctttgg taatagtgta gtttttctga
       61 aaactccata ataatgaaat ttgtgtatca tgttatgttt attatggaac gttagtcaag
      121 aacaaaatgc aatttttaat aaatccatat agtgaacttt gagttctctt tatcctttta
      181 tcagcaaagt atgtaacttc attcgttgaa ttattaagac cgccttcaat agacgaaggt
      241 gccagagcaa cgcttcccag acgcgaggct gcggtttgcg gattttgact ttgaccatgg
      301 aattcaaact cgctctggaa gcacagaaag gatggaggaa acttcgaggc tataagattt
      361 gtcaagttgc gaatggtgag gagtttgtaa atggcaaact ggataatatc gcatgaaatt
      421 ttttgagggt ttgttaattt tttcatccac aaaatttaac gataagtcga gccgtttgag
      481 gtggctccac agtgttggca cgggggtatt gagcgcataa atatcccatt acgaagtgat
      541 aaaacttttg tttcccatca atataaaata aagcatgaga aaaaatatgg agattttttt
      601 ttgaataaaa ttgttaatta tggcaacttg aacatagtga agaattttat tcctctttga
      661 aaaaggttag caccctcatt ctgaggcaaa tccgaataat ctctccctgc taaaaacaat
      721 aaattcttca gttgctatca ctctatcaag gctctgaccc caaaaaatat gttatataca
      781 aaaaagatct ataattttta tttacaatag tctaacataa ttgtgcagta aaaaagcgag
      841 ggtagaggta tacaggtaaa gtcaaagcat atgccttgac aaagtagcag ataaaaaata
      901 taatggggtc agagccatgg ataacagcac agtaacacta aatctggcat actatactaa
      961 aaaactgcat gatttaacta aaaaatcgtg gtattactgt gcaaagagtg tttataaaaa
     1021 ccgaagatta ttggcgggca ataaccctgt taaaaccata ttcaaatcaa aatccatact
     1081 tattgctata tacaataaat gtaaaaagga aaactttacc cattcgcaat ttactccact
     1141 tgattttgct tttatacaaa tttttattga tacatattcg cgtgatgttc ttgaaaaggt
     1201 ttttttacat tccatagctg ttgaactatc acagggacag tatgcaatat gtacatccac
     1261 tttttgtgat gataatttta cactggatga cattgtacat tttaacaaaa cagccagcat
     1321 tacaatacta tgtcatagca atgaaacagc aaaaagttgg ccaaaaaaca atatatgcct
     1381 tgagtggtat tttagactac tggagccgtt aagcaattat acagcattta cccggtggct
     1441 tgctaaactt ttttacaata acaccattta ccaaaatgcg cagggcataa ttacaagcta
     1501 tgatacattg gtaaacacct ttgctaccta taacagaact actgctgcag atgttatacc
     1561 acttttgtca aaaaataatt tctggaaatt tcctgatagc gatgaattat atgagcttgt
     1621 gtttgattgt gaggttgcag ttaaaagcac tgatatccag caggttggtg ctttgctgtg
     1681 ctgcaggggt ctggtaatca gtactattga ttcgctgagc cagttaaaaa agctggatgc
     1741 tatatgcatg ctcagggtca ttggttataa cattctggat tttgatttag acatactatc
     1801 gcacaataat ttttcttttt caaatcccta tgagatagtt gatctattat gggtgtacaa
     1861 catcctttat ccaatcaatg atggctcatc gctggatgat gcgttcaggc gtttggttgg
     1921 gatgtccatt gaaaagcgtc atactgcaat agccgattcg tatgctgcat gggatgtgtg
     1981 gtgcaaaata aaagagctta catcatttca taaagggcat tttttaaaac atcttaccaa
     2041 tgtaacagct atagcacatg acaaaaaagc aaaacgtcat tgtggcgata gtttgcgtaa
     2101 atttattgca tcccatacaa aagggattat agaatgtaat gataccactg atattataca
     2161 acagcttata gctgcaactc ccgacgacca atttgctttt atttttaaaa cagcaaagga
     2221 tatgcgaaat accgttgcgc aattgttatt ggataaaatt attaccattg atgatataag
     2281 caattcagta tttttagatt cagaagaatt ccatattgat agcgataagc ttaaacaggt
     2341 aaaaccatcc catgatgtac tacagctgta tattcaatca ctactactgt acaatgtaag
     2401 tactacctca cttatttcgc ttcctctgtt tattgctgag cataagcagg gttcattgat
     2461 gcatcagctg caaactatta tggaaagctg tatatgccat ggtagccctg atattaaaag
     2521 caatgcaaaa tatgtttttt taaatatcgg tggttttaac accatgaaac ttagtaaaac
     2581 tatatttgct tcacggcatg tagtgtttaa cagcatacat caatacgata acctttttac
     2641 caggctcctt tcaaaacagg ctgatataga acttaccagt gacattatta ataagcttgg
     2701 gcttgaggat agcaataaag tgtatattac acgcgattat attatacaca atactgccta
     2761 taataattat gaacaatttt tacatgaact tttacccctt atacatacca gcgatggtaa
     2821 atgggcaaca acacttttta ccaggataaa tacgttaaac aagtttgagt atgttccctg
     2881 tataggtaaa gcgggcaata catggaaatt gatttttgaa ccgctgtact ggaaggcaga
     2941 tattgatacc ctcttttcag aggcttcatc catgcacttt ttagcacact ttgtagataa
     3001 atctaatgag ggcgcattat gctataaaat aatgtttggc atagacgatg atgccatttt
     3061 tgaaaaaaaa gataaacttg ttccggtaaa gctaaaggcg taccgatcaa atttaaaaca
     3121 aacaaagttt aatactgcgg tgtatcgtaa aaatgcactt gacatcatcc ataaccttgc
     3181 cgggacagat gctataaaag ggaaaattgt tattatatgc actaacgatg aagagcgtga
     3241 tttttactac aactattgta cgcgatattt taataccact atctttagaa ccgataccat
     3301 ccccccgcgt ttttttgaaa agaatataac ttaccatagt aattacattg ttattactac
     3361 catatatgat gttaacagta taaagggtgg caatattgat tatgttatag taccccgttt
     3421 gtcgctgggg tatgatgctc cgtctattaa ccagtggcga aaacaacata tagaggagtt
     3481 gttgttaaat gaaaatattg aaatgttgca actatttgtt aagcactttg gtagtgcaga
     3541 agcggataag gtgctgggag cctttatata tacctaccct tttttaaatg ctatgaatgt
     3601 actatatacc atgcttttaa ataataccat tgatactgtt gccgttacac ttatggatac
     3661 cagcagctta ttattaccag gaaaccagcg aatgcataaa aagtttgaag aaatttttaa
     3721 aagcaatgac ctggttgagg aaagcatggt gcatgttgaa aacctggtaa aaaatgagct
     3781 tgttgatgaa attatgggta aagagtattt acaattaaat aaagaccttt acaccatgtt
     3841 attgcacaaa cactggggta aagacaaaac ctttaagata tttaataaag acataaaagc
     3901 attcccggat aaagaatata tcatcagcaa tagtggtgtt ataaggcagg atgatattat
     3961 ccattatgtt atgcgttcat tccatgatcc caattgtaag gataaagatt cgcttgttat
     4021 tgcagctact ggcattggaa agtcgctcat atatcaaata cctgcaatta tcctttcaca
     4081 ggagttagaa ccagagctaa caatagtaat atccccgctt attgcattga tgaaggacca
     4141 ggttgatgga ctgcacaagc atggtgttta ctccgttgct cagtttaatt ccactctgga
     4201 aagtgaatcg cgcagtgtaa taaaaaacct tattttagaa ggttttataa atattatata
     4261 tgtatcgccc gagatgattg ttcagtcaaa aaattttcag gatatgatag gacggcgccc
     4321 gccctcattt ttggtggtgg acgaagctca tagcttatcg cagtggggac atgatttccg
     4381 tattgattat cacatagtag caagcacaat aaagcaaatt tttaaatacc gtgattttcc
     4441 tgttgcagcg tttactgcaa ctgcaaagat aagcaagttt gaaaacctat cggttgtaca
     4501 tgatattata aaaatcatgg agatggatat atcccgcgac agagcgctgc tgtgtacaac
     4561 aatacgtgat aacctttatt atcatgtaaa gacatttgaa tctggaacta aattagaaaa
     4621 ttataatctt gatgaacaaa aggttacatt tttggtaaat ttttttaaaa atcctgcacg
     4681 gtatatagca aatgaacata agcaatcaat acttaaagat acatctactg ataaaaaata
     4741 taaaactatc atctactgtt catcggttaa cttaaccaat acattatctg aatatttaac
     4801 cagcaatacc aattacacgt ttaagccatt taatgcaaaa cttgaacaat accagaaaga
     4861 agacatacag aatagattta aagcgggtga tggcattgac ggtatatgtg caacaactgc
     4921 ctttggtatg ggtgttgatg tgccagatat acgcatagta atccattatg atttacccca
     4981 gggtattgaa gcctactatc aggaatcggg aagggcaggc agagatggta aaaactcata
     5041 ctgcctttta ctgtatcaaa aaaaattgat taccaaaaat acatccaata aattggaaaa
     5101 tgactatcaa agagttcaat atttaatggg aagggatagg atacggttgt atgatttctc
     5161 aactatttat aatggcattg cagtattttt gcaacagcag cctgataaaa gtaaagactg
     5221 ctatgttaat ggaaccatgc tccatgcact ttttattgat caaaataacg atgcttccaa
     5281 taaggtggat tcacttcttt tttatatgag cgactattac catggtgata tgaaaggata
     5341 ttggtgctat tcaaaggaaa aggcatcatt aacctataaa atacttaagc caaagattca
     5401 ttattccttt accagtgaca gtacattaaa taaggtaatc tggagtgtgc ataaatgcct
     5461 ttgcaggtac aaagaacaac aaagcccgga aagcaaaagc tatgttgaaa ttacacatca
     5521 acatttaaat aacatgctaa aaaaggatgg caactatcat ttcagtcaaa atgatttata
     5581 ccgggcatta agcactttga taacacatga ggttatacgc atagatgatg ttaaaagcta
     5641 ctatttgaac gataaaaaag ctgtgggcgg attaacagca ttatttgata aaatgataaa
     5701 ggtatgcgaa aactacagcg tttcaactaa acaggatggt tatggcacaa cctgtagaat
     5761 agatttcaga aagatgttaa ctggtgatca taacaaaact ataaatatga atacgcttga
     5821 gcttatagta tcattaatag taaaacagta tattgacggt aaatataata aaaatacgag
     5881 aaataagaaa aataaaaaca atgaatttgt aaccataaca atgaagtacc ctgcattaaa
     5941 ttttatcctt cacagagatg caggtgaaga taaactcatt gaggctatag atgggttgaa
     6001 atacaccttt aaaaaataca gggatgaaat aatagggata atagaatttt tagccagccc
     6061 agtatgcgcg acatcaaagc gttgttcaat atacgagtta tggaccagat acgaagaaga
     6121 ttgtgttaac catggcaaaa aaagtgtcaa cacatataca tttcgttata atattggttt
     6181 gcttcaaata cttgggattc tggactcatc aaataataac gattttgaac caagctatac
     6241 ggtaaaagta tatgacatca ataaatcgcc tttagagcat atcaatacag cgtgggaaga
     6301 gattgaaact atctacaaca ccaaaaaggc atcaatgaaa ctgctggtgc aacaatatat
     6361 attggatggg ctatctacta aagatggtaa agatggtaaa gatggtaaag atgataaaga
     6421 tgtttttgat aaacggcgta atttttttga agaatttttc ctgaaagata gttatgataa
     6481 aatgttgcta atacaaaaca aggattttgg tgagcaagcg tgtgaataca ataactgttt
     6541 tttatcaatt gctggtacta ctgccaacac ctttaaaaaa caagatggta aatacactat
     6601 tgaagatgtc attgcctata ttgcatcaga agtaaaaaaa ttgcaaaaac aactaataca
     6661 acagtatgtt gtcagcatta aaaagccaga tgatgtaccg attattactc ttaaaagtgt
     6721 catgtatact gagtttatgc ttttgctgtt agaatccatc aggataccgg tacataaact
     6781 atcagactgt gttaccataa caaaaacaag cgagcatcgc gatgcaatta ataattatga
     6841 agcgtttgac aggcaacagc ataaagccta tacagttgca agttttatac tctatatgct
     6901 ggttattagc tcttatttta cagggtttga aacctttgac aataaagatt atatagaata
     6961 catgcaaaaa aataatggca cacggcattt agatggcggt gttactatgg atagtgataa
     7021 attttgcaaa gaagttgttg aagaagctgc cggatatgta aaaaaagaat taaagaatac
     7081 aggctataag aatatcacct atcaagagat agctcattat gctgaacaat tttttactac
     7141 acggtatgtg ttattcaaca actataagaa agtaccaaca tcgccctcaa caaattattc
     7201 atactacagc aatgaagcat tgtcaaaaac tatcgataat tatattttta agcagcagtg
     7261 tatcttcagg gtttttgatg tgtataatag gttccagaat caaaatgctg tagaaaagca
     7321 ccttgcttca tttgaaatag aaaggttttt gggtaactat cttgccaatg ccatacagta
     7381 ctacgtcaac aacaataata acatataccc ccaaaacata ctgccaatga ttaaccattt
     7441 tgccattgta gtagtccatg acattgaaaa taaatgggtt aatattataa aattgtttaa
     7501 tccccatgca gttgtgataa agataaacga tgcaattttt aatggcgata gtaaagatga
     7561 taatgcagta tatcaatata aaaaatgcga ggtgttgtat gaagaaagtt atgtttataa
     7621 ctgtggggac aggcagagaa cgtacagata tagcacaggc aatagtggaa agtatacgaa
     7681 tccaaagccc tgactttgtt gtgttttatt gtactcacaa aagtagcaat gaaaccatgc
     7741 cagaaatata tgcattaaac aatgccttgc aggtaaaaag cacagttgaa attataagtg
     7801 atgaaaataa tcttgaaaaa ataaaaaaag agattagcca tagcatggca cagtataaaa
     7861 agaattatgc attaaatacc gttgattaca cgtcgggaac caaggcaatg tcggcgggta
     7921 ttattgtagc tgcaatagaa aatagctgtg attatatatc ctatataact ggcaccaggg
     7981 ataatcaggg acgagtagtg acaggtactg aacaaataaa tacatttaga ctcaatcaaa
     8041 tttatgcaac aaagtatgtt gagcttggaa aacaattttt taataactat ctgtttggag
     8101 cagctattga tttatttgaa aaagcaaaaa atatatgtca tgatgaaacc cttaacaatg
     8161 aagtagaact ttatatatcc cttgcacagg ctttgaatgc atgggataaa tttgatcatg
     8221 aacaggcaaa aaatattttt accgcaataa caaaaaataa acaggcagtg gtattacttg
     8281 aaagtaagga aattgaaaaa acaatagaaa taatgaaacg acacctgcac gctatagcaa
     8341 gtagtcaatt ttctgattta ttgattatag atttattgaa aaatgctgag aggcgctttg
     8401 ccgaaggaaa atacgacgat gctatggcac ggctgtaccg cttgcttgaa tttattgcac
     8461 aggtgttgct ggataaacaa aatatatatc aaaaagatgc tcatggtaaa agcgatacca
     8521 gcaaacttga tgttgaaaaa ctgccgcaac atttaaaaga aaaatataca ccagcaattg
     8581 gattaagtaa atcctatgag ctgttaagtg atttaaatga tgaaacaggt aaaaagattt
     8641 atgatataat gcaaaaaaag gatgtaaaac aaatactggg catacgaaac ggctcaatct
     8701 tggcacatgg ttttgtgcca gtatgcagag atataacaca gaaatgtata gaaattatta
     8761 aagaaatagc cagcatttat tttacactac agcagtttga aaaattatat catgaattgg
     8821 agtttattac attggagtaa tactatggca gatatttatt tgatatctga ttacgggaaa
     8881 ttgtataaac aaaacaaggt gtttcacttt ttatatcccg acggtactgt agcaaaattt
     8941 tttccgcata atacacagcg tatgtatgtt attggcaata ttcaaattac tgctgaagcc
     9001 ttaaagatga taagccataa taaaatagag gttatatttt taaataaaaa tggttttttt
     9061 aatggcaaac ttacctttgg tgacagtaaa aatgtattgc tacgtaaaaa acagtatgaa
     9121 aagctcaatg acaatgattt tattttaaaa tggggtcctg cgggattaaa ggaattcagc
     9181 gaaggcacgg atgccgtagc gtcatacggc tc
//
LOCUS       DAOOJH010000095         9136 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6653_length_9136_cov_3.885035, whole genome shotgun sequence.
ACCESSION   DAOOJH010000095 DAOOJH010000000
VERSION     DAOOJH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6653_length_9136_cov_3.885035"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..986
                     /locus_tag="PLJ75_10380"
     CDS             318..986
                     /locus_tag="PLJ75_10380"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HPD05659.1"
                     /translation="MPDIIVKTNLIYLVAIFFVIFIIREIVAFRSILKLKYIFTPLIT
                     YIVIVIALYLSYISGFSQYDIFIIAGLVFALIADTFLMIEEINYFIHGLLFFLFMHVC
                     YIIALSIHYQFNYLDIIILAIIILISFSYYAVIHKAKGRLYVPVIIYMIVLSAVVLLA
                     IGTFIKTMSPKGALVMLAAIFFAVSDGILACNQFVKKIPHSTVITWSLYAPAQLLFAL
                     SCYY"
     gene            1092..2153
                     /locus_tag="PLJ75_10385"
     CDS             1092..2153
                     /locus_tag="PLJ75_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPD05660.1"
                     /translation="MKKYIMLLLLLSVSCSQLIKPDSKREYENIYKHLTNAESLENPV
                     QKEKLLTRAYGIIQQSALTKEEKTGSRLLALLSYYYFLKGNYKDAEVNIGSAQPLSAD
                     DPFMAVLQARIMLATQGKSGVQAALGLLSPYEKYDNPMMYIATGDAYFIKGDYTTARD
                     NYKKALLLDKELQVVAANRLEVISRISTLTIDVSKVSDFILQPAMTRDKVAYLMYEIF
                     EIQKYISAVKPLDANFIDIEKSHYSTAIINLRKKGFFSYIDGGSFEPFMIVSRGEMAK
                     IVEDFLVLSKNNEGLRKKYAHESPSFFNDSKHDNPYYNALRLACDVGVMSVSLSQDAY
                     PDEAVTGLQAIMIIQKLVK"
     gene            2162..7075
                     /locus_tag="PLJ75_10390"
     CDS             2162..7075
                     /locus_tag="PLJ75_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05661.1"
                     /translation="MKKTLAIYSCVIAAFIAGVLAYAQFVTNTDKGQDERQKNKVTYK
                     NYIWIEGENAVSTNFAREPIYNFFCSNRFALQLAKDVDPPADGYYANYVFYVTHTKEY
                     DLWLGCTPPGSMYHDRPGYASPIEWKVDNGPFQSASAENVYVKDFYGIGGFYWVKIAT
                     GTLNAGKHNLTIRVKQKRSSGWDYYFYIDAILFLPHYSIDIAELMNFPEVAPKNFTTP
                     GEGILFDDINEYEKKIRTNINDRGTLFTLVQIYGWLYDYNKAIVLLQRYLEKNPRDIQ
                     MRLLLAANYSWSDQLDAAINEYKNIIAIDEKNITARKLLSVLAGWNNRYDEAIQYYQQ
                     IIAIEPDNVDAYISLATQYSWKGEMDKAIVTFEKAESIAPDNVAVLEALGDNYYWLGR
                     NYDAIRQYYRIIDLDEKNISAYKKLAKIYLDVGETAKASRVINDAKQIIDLYPELSGI
                     SLDVKDELAKERRELINSYKEALVKTPEDVNLRKGLIDTYIWNKMHSDAVKEYDTLLT
                     YKMFHSIEESEQKLTRLIIHTAGLQAAKPVIASIKNDLSNISKRYAKHIENQKSGKMV
                     VDDAQLSHDMALLTRYNDKLKAVQHELQLYNDITAALSKDVQTYMAMQAATGWRFEPG
                     RIGNYADTAMNMYPQMYQPYKVKGIVALFYGKPSDAVTGFEKVKQLTGNVSPTLSLAY
                     AQTKDYKGAIVTLEESLQNKTIASAYKLDIEMVVSMASLPGPEVTRTQSSLPDIENAL
                     ADMHAIIDSVAHELEQKVVSGLTTMREVYEKGFLDLEQENVRIYKEIANYYMQKGNYN
                     ASRDYYNNILKVQPLNVEVNFSMGNINELLGYWKDAEKNYEICINAQPDFEYARKAHY
                     QLQILHAPSLTPDIHYFSDNSIDRYSAGLSCGYELNQFLGLRAGYSYGKQTDKGGIST
                     TGQLFYPSEGDVDMQKVFVAAEIPVRMLYSRLIFNVNGTFYSGTVDYGNPLYDTSLSY
                     FSPGYGAGIIITPPATGVTLKLTYHHDDENDLTQSLKMKFRDAITYDSFEGVLDINFY
                     EYNFPLSDRISLFNAIEHRKLSDSNTRLSSFNQFTFRLYRIPKINMVFDLAGIYSYED
                     TDFNKYSSGDILLVPYWAPMGVSYYGPMLKVSQQIDNLFKGGLYYSLFVEYLYNSLEQ
                     KTVMPGISLVQTWDKIQIFMDYIYTSVTLPDSATEDTYVSHDFRFGATGKFFTIYTPK
                     GAGGKPILFVSANPILITPDGDGKDDFTTLNLTAFDEKGISQWWIEILNEKGEKIQEY
                     SMAGAVPATYRWDGTNLMNVLAPEGKYYVQLSIQNTAGERFTSKKQEVFLATAKRAVA
                     LTPSYKEFSPNGDKVKDMVSFTILATDKKNVNSWFMTISSKNRVIKTIRGNDFIPYDV
                     MWDGNDDSLKVANDGEYSIVMTMQYDDGITIKSAAATVTVVTRVTVDIKVEPAEIVPA
                     QGTLRIMPAVDGKIEQWQVRILRDDGALLKIINGAGMPPKVVAWDGTDEKGEPVAYNM
                     PAKVEMEVTDTAGNTSLSNKVPFMIGFLMKQEKGKRVLYVFNQDIMFKGRSDELTGTG
                     VFVLGHLQAKLATISGFTTLSIVAYTDDVGSSQANMELSQRQAQVIADNMKGVNAQIK
                     ALGAGSQILYLKDKPSKWDMRYEIELY"
     gene            7056..>9136
                     /locus_tag="PLJ75_10395"
     CDS             7056..>9136
                     /locus_tag="PLJ75_10395"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HPD05662.1"
                     /translation="MRLNYIKLKPYRVTIAITCVMVLFVPLNIFAKAQGHTVLCLYKS
                     SEGYNDDSNPLKWFFEKDITSNGLTVRYYDFDKGFTPLSDLKDVRAIVTWYNTGVVNS
                     RDIGINYARFMTQAADDGIKIIIVNSYGAYGYNDGSEVKWDLLPYIRPLFTKIGIFFQ
                     GFWTNNPQNITILYKDSAMVENDEKQDVSKSLHYQQVIPLREDVKTYLQLQRLDAPPQ
                     AGDGKSSVIIISKTGAFALENYVVRGSKVMLNTSAFIKEALFYDDGYLNVAVFIGDKS
                     NADKVENNIRHAFNYAKIRYAIFSKKDSENLVVHDLYEYEAVLVATKTKDAIPFALLK
                     QYVAEGGRCVFINYIDPDPELNQLIGAKQYAKKAEYFKQGVYIQKQFFINAAAIPGDD
                     MDCNVRMAVLDNDTTVLGDVISLFDAERYPLVWERRYKNGKILYWNTDLLDESKLTRG
                     MIVQSVYKIAGVFATGMINVGIMMIDDFPAPWWNVAYKPYRIKYYNDLLQNEKDKFNR
                     KKLEEIIAKLKNLPEETDTLFISDVWLHDILGWQKRFGFVYSSFLIFNYNKDTKGDEI
                     GFSVRDFYLSQNGLSTRMGLAALENGFELGLHGYNHMSLTLTKPMYYESTPWPDKKAM
                     VDALSVTKNEWILLYGESALPFSYVAPHNIIDTIGLQALGEVFPSIRVVATLYVAKSG
                     ESEQEFEWSPD"
BASE COUNT         2899 a         1487 c         1987 g         2763 t
ORIGIN      
        1 aatccacaat gtacccgtct cagcagggag gtggtgccga tagctggcat ggtgccatcc
       61 ataagggatg acgatggatc gttattgcca ttctgaggca aagccgaaaa atcctgtcgt
      121 tcaaaggata gatttttctg tagctaacgc tccttcagaa tgacaatgtg cggattgtta
      181 tcctgaacgc tgattcagga tctaacgtta gacatctgga ttccgtatca agcccggaat
      241 gacagtgaga aataatgcac ttttttgctg ggaagtccag ggtgaggtta ataatttttt
      301 taaggaaggt ttaatatatg cctgatataa ttgttaaaac caatcttata tatcttgtag
      361 ctattttttt tgtcatattt atcatacgtg agatagttgc atttagaagt atcctgaaac
      421 taaaatatat ttttacaccg cttataacct atattgttat tgttattgca ctctatctgt
      481 cgtatataag cggtttttca cagtatgata tttttattat cgcagggctg gtatttgctt
      541 taattgcgga tacgttcctc atgattgaag agataaatta ttttattcac gggttgctat
      601 tttttttatt tatgcatgta tgttatataa ttgcactgag catccactat cagtttaatt
      661 atcttgatat tattattctt gcaattatta tattaatttc tttttcatat tatgctgtta
      721 tccataaagc gaaaggcagg ctatatgtac cggttattat atatatgatt gttttgtctg
      781 ctgtcgtatt gcttgcaatt ggcacattta taaagacgat gagtcctaaa ggcgctcttg
      841 taatgctggc tgctatcttt tttgcagtat ccgatgggat actggcatgt aatcagtttg
      901 taaaaaaaat cccccatagc actgttataa catggtccct atatgcccct gcacaacttc
      961 tatttgcttt atcctgttat tattaatgct tttggtattt ttttcttgta agttgcattg
     1021 cctgttgtat atactatgaa catttgatgt taaggctgca gtggcatata tcaatataaa
     1081 tggtggaata tatgaaaaaa tacataatgc tgctactgtt gttgtcagtt tcatgttcac
     1141 agttaataaa acctgattca aagcgagagt atgaaaatat atataaacat ttaacaaatg
     1201 ctgaatcgct ggaaaatcct gtacaaaaag aaaaacttct taccagagcg tatggtatta
     1261 tacaacaatc agccttaaca aaagaggaaa aaactggcag cagattgttg gctctcttaa
     1321 gctattacta ttttcttaaa ggcaactata aggatgcaga agtaaacatt gggagtgcgc
     1381 agccattatc tgctgatgat ccgtttatgg cagtgttgca ggcaaggatc atgcttgcaa
     1441 cacaaggtaa atcaggtgtg caggctgctt taggattact atcgccctat gaaaagtatg
     1501 acaaccccat gatgtatatt gctacaggtg atgcttattt cataaaaggg gattatacta
     1561 cagcacgcga taattataaa aaagctttac tgctggataa agaattgcag gttgttgcag
     1621 caaacagatt agaggtaatt tcacgcataa gtacattgac aattgatgtt tcaaaggtat
     1681 cggattttat actccagccg gctatgaccc gtgacaaggt tgcctacctt atgtatgaga
     1741 tttttgaaat acaaaaatat atatcagcag taaaaccact ggatgcaaat tttatagata
     1801 tagaaaaatc ccactatagc actgccataa ttaatttgcg caagaagggc ttcttttcat
     1861 atattgacgg aggtagcttt gagccgttta tgatagtttc aagaggtgaa atggcaaaaa
     1921 ttgtagaaga ttttttggta ttatcaaaga ataatgaggg attgcgaaaa aaatatgcac
     1981 atgaatcgcc gtcgtttttt aatgatagta aacatgataa tccctattat aatgcattga
     2041 gacttgcttg cgatgtgggt gttatgagcg tttcgctaag tcaggatgct tatcctgatg
     2101 aagcagtaac aggtttgcag gctataatga taatacaaaa attggtaaag taggggtgcg
     2161 catgaaaaaa acattagcaa tatattcatg tgttattgct gcgttcattg caggggtttt
     2221 agcctatgcg caatttgtta caaatactga caaggggcag gatgaaaggc aaaaaaataa
     2281 agtaacctat aaaaattaca tctggattga aggtgaaaat gctgtatcta ctaattttgc
     2341 ccgtgaacct atctataact ttttttgtag taacaggttt gcattgcagc ttgcaaaaga
     2401 tgttgatccc ccggcggatg gctattacgc caattatgtt ttttatgtaa cccatactaa
     2461 agaatatgat ctttggttgg gatgcacacc tcctggctca atgtatcacg atagacctgg
     2521 ctatgcatca ccgattgaat ggaaggttga caatggacct tttcagagtg cctcggctga
     2581 aaatgtttat gttaaggatt tttatggtat tggtggattt tactgggtta aaattgccac
     2641 aggaacgctc aatgcaggga aacataatct taccattcgt gtaaagcaaa agcgaagcag
     2701 cgggtgggat tactattttt atattgatgc gatactattt ttaccccatt acagcattga
     2761 tatagccgaa ttgatgaatt tccccgaagt tgctcctaaa aattttacta ctccaggtga
     2821 aggaatactg tttgatgata ttaatgaata tgagaaaaaa atcaggacaa atatcaatga
     2881 taggggaacc ctttttacgc tggtacagat ttatggatgg ctttatgatt acaacaaggc
     2941 tatagtgctt ttacaacgat atttggaaaa aaatcctcgc gatattcaga tgcgactgct
     3001 tttagcggca aactattcat ggtctgatca gttagatgca gcaataaatg aatataaaaa
     3061 tattatagca attgatgaga aaaatatcac cgctcgtaag cttttatcgg tgcttgccgg
     3121 atggaacaat cgctatgatg aggctataca gtattatcag caaattattg ccattgaacc
     3181 ggataatgtt gatgcctata tatcgcttgc aacacaatat tcatggaaag gtgaaatgga
     3241 caaggctatt gtcacatttg aaaaagctga gtcgatagct cccgataatg ttgcagtatt
     3301 agaggctctt ggtgataatt actactggtt aggcagaaac tatgacgcta tacgacagta
     3361 ctatcgtatt atagatttag atgaaaagaa tatatccgca tataaaaaac ttgcaaaaat
     3421 atatctggat gttggtgaga cggctaaagc cagcagagtt attaatgatg ctaagcagat
     3481 cattgacctg tatccggagc tttcagggat atcccttgat gtaaaagacg aacttgcaaa
     3541 ggagcgaaga gagctgatta atagctataa agaagctctt gtaaaaacac ctgaagatgt
     3601 caatttgcgg aaaggcttaa ttgataccta tatatggaac aaaatgcatt ctgatgccgt
     3661 gaaggaatat gacacactat taacctataa gatgtttcat tcaattgagg agtctgaaca
     3721 aaagttaacg cgccttatca tccatactgc aggattgcag gcagcaaaac cggtaattgc
     3781 atcaataaaa aatgatctga gtaatataag taaacgatat gcaaagcata tagaaaatca
     3841 aaaaagtggc aagatggttg ttgatgatgc acagctatcg catgacatgg ctctcctcac
     3901 aaggtataac gacaagctaa aagcagtaca acatgagctg caattatata atgatataac
     3961 tgctgcattg agtaaggatg ttcaaacata catggccatg caggcagcaa ccgggtggcg
     4021 ttttgagcca ggaaggatag gtaattatgc tgataccgca atgaacatgt accctcaaat
     4081 gtatcagcct tataaggtta agggtatagt tgcgttgttt tatggtaagc cttccgatgc
     4141 tgtaacaggt tttgaaaaag tgaaacaatt aaccggcaat gtatctccaa cactgtccct
     4201 tgcctacgca caaacaaaag attataaagg ggcgatagtt actcttgagg agtcgttaca
     4261 gaataaaaca atagccagtg catataagct ggatattgaa atggtagtat caatggcttc
     4321 attgccagga ccagaagtta cccggacaca aagttcgttg ccagatatag agaatgcttt
     4381 agcggatatg catgccatta ttgattctgt tgctcatgag cttgaacaaa aggtggtttc
     4441 tggattaaca acgatgcgtg aggtttatga aaaaggattt ttggatttag aacaggaaaa
     4501 tgttcgtatc tataaagaga ttgcaaatta ttatatgcag aaaggaaatt ataacgcatc
     4561 acgtgattac tataacaata ttttgaaggt gcagccactg aatgttgagg ttaatttcag
     4621 tatgggcaat atcaatgagc ttttgggata ctggaaggat gccgagaaaa actatgaaat
     4681 atgtatcaat gcgcaacccg attttgagta tgccaggaaa gcgcattatc agctacaaat
     4741 actccatgct ccatcattaa ctcccgatat tcattatttt agtgataaca gtattgatag
     4801 atattcagca ggattaagct gcgggtatga gttgaatcag tttttaggat tgcgcgcagg
     4861 gtatagctat ggcaaacaaa ccgataaagg ggggatttca accacagggc agcttttcta
     4921 cccatcagaa ggtgatgtcg atatgcagaa ggtatttgtg gcagcagaaa ttccagtacg
     4981 catgctctat tcccgattaa tttttaatgt caatggtaca ttttattctg gcactgttga
     5041 ttatggtaat ccactttatg atacatcatt aagctatttt tctcccggct atggcgctgg
     5101 tattataata accccgcctg caacaggtgt aacattaaaa ttgacatatc atcatgatga
     5161 tgaaaatgat ctgacgcaat cacttaaaat gaagttcagg gatgcaatca cctatgattc
     5221 ttttgaaggc gtgctggata ttaatttcta tgaatataat tttccactga gcgaccgtat
     5281 ttcgttgttt aatgcaattg agcatcgcaa gctatcagat tcaaataccc gcttatcaag
     5341 ctttaaccag tttacattca gactataccg tatacctaag attaatatgg tgtttgatct
     5401 ggctggtatc tattcgtatg aagatactga ctttaacaaa tattcttcag gagatattct
     5461 gctggtgcca tactgggcac cgatgggtgt ttcgtattat ggcccaatgc tgaaggtatc
     5521 ccagcagatt gataatctct ttaaaggtgg attgtattat tcattatttg tggaatatct
     5581 atacaattct ttagagcaaa aaactgtaat gcccgggata tccctggttc agacatggga
     5641 caaaatacag atattcatgg attatattta tacaagcgta acccttcctg attccgcaac
     5701 cgaggatacc tatgtttcac atgattttag atttggtgca accggaaaat tttttaccat
     5761 ttatactcct aagggtgctg gtggaaaacc aatattgttt gtatcggcaa atcctattct
     5821 tattacccct gatggggatg ggaaggatga ttttaccacg ctgaatttaa ctgcatttga
     5881 tgaaaaagga atatcgcaat ggtggattga gattctgaat gaaaaaggtg aaaagataca
     5941 ggagtacagc atggctggag cggttcctgc tacctatcgt tgggatggca ctaacctcat
     6001 gaatgttctg gctccggaag gtaaatatta tgttcaattg tccattcaga atactgccgg
     6061 cgaaaggttt acctcaaaga aacaggaggt ttttcttgca actgcaaaac gtgcagtagc
     6121 attaacaccg tcatataagg aattttcacc taacggtgat aaagtaaagg acatggttag
     6181 cttcacaata ttggcaacag ataaaaaaaa tgttaatagt tggtttatga caatcagcag
     6241 taaaaacagg gttattaaaa ccatacgcgg gaatgatttc ataccgtatg atgttatgtg
     6301 ggatggaaac gatgatagct taaaagtagc caatgatggt gagtactcaa tagtgatgac
     6361 aatgcaatat gatgatggca ttacaataaa atctgctgct gccactgtca ctgttgttac
     6421 cagagtaacg gttgatataa aagttgaacc agcagagata gttcctgcgc aggggacatt
     6481 gcgtattatg cctgctgttg atgggaagat agagcaatgg caggtaagga tactgcgtga
     6541 tgatggcgca cttcttaaga ttatcaacgg cgctgggatg ccgccaaagg ttgttgcatg
     6601 ggatggaacc gacgaaaagg gtgagcccgt tgcctataat atgcccgcga aggttgaaat
     6661 ggaggtaact gatactgccg ggaatacatc cctatcaaat aaagtaccgt ttatgatagg
     6721 atttttgatg aagcaggaaa agggtaagcg cgtgctctat gtatttaatc aggatataat
     6781 gtttaaggga aggagtgatg agttaacagg cacaggagtg tttgttttag gtcatttaca
     6841 ggctaaactt gcaacaataa gtggtttcac aacactctct attgttgcct ataccgatga
     6901 tgtcggttca agtcaggcaa acatggaact atcgcaaagg caggcacagg tcattgctga
     6961 caatatgaaa ggggtgaatg cacagattaa agctctgggt gcaggcagtc agatattgta
     7021 tctgaaggat aagccatcaa aatgggatat gcgctatgag attgaattat attaagctaa
     7081 aaccatatag agtaactatc gccattacat gtgtcatggt attgtttgtg ccgttaaaca
     7141 tttttgcaaa ggcacagggg catacggtat tgtgtctata taaatcgtcg gaagggtata
     7201 atgatgacag caatccgtta aaatggtttt ttgaaaagga tatcacctcc aatggattaa
     7261 cggtgcggta ttatgatttt gataaaggtt ttactccctt atcggattta aaagatgtac
     7321 gggcgatagt tacctggtat aatactggcg ttgtcaatag cagggatatt ggtattaact
     7381 atgccaggtt tatgacacag gctgctgacg atgggataaa gataataata gttaattcat
     7441 atggagcgta tggctataac gacgggagtg aggtaaaatg ggatttactc ccgtacatcc
     7501 gcccgctgtt tacaaaaatt gggatttttt ttcaaggttt ctggacaaac aatccgcaaa
     7561 acatcacaat cctttataaa gacagtgcga tggtggaaaa tgatgaaaag caggatgtaa
     7621 gcaagtcatt gcactatcag caggtcattc ccctgcgcga ggatgtgaaa acatatctgc
     7681 aattacagcg gttagatgca cctcctcagg caggtgatgg caaatcaagc gtaataatca
     7741 tttcaaaaac aggcgccttt gcactggaaa attatgtggt tcgtggcagt aaagtaatgc
     7801 ttaacacctc tgcatttata aaagaagctc ttttttatga tgatgggtat ttaaatgtag
     7861 cggtttttat tggtgataaa tctaatgctg acaaggttga aaataatatc aggcatgcat
     7921 ttaattatgc caaaatacgg tatgctattt ttagtaaaaa agatagtgaa aatttggttg
     7981 tgcatgatct atatgaatat gaggcagtcc ttgttgcaac aaagacaaaa gatgctatac
     8041 cgtttgcact gctgaaacaa tatgttgcag agggtggcag gtgtgttttt ataaactata
     8101 tagatcccga tccggaatta aaccagttga ttggtgccaa acagtatgca aaaaaagcag
     8161 agtatttcaa gcagggtgta tatatacaaa aacaattttt tataaatgct gctgctatcc
     8221 ctggagatga tatggactgt aatgtacgca tggcagtatt ggacaatgat acaacggtac
     8281 tgggggatgt tatttcactg tttgatgctg aaagatatcc tcttgtgtgg gaacgacggt
     8341 ataaaaatgg gaaaatattg tactggaata ccgatcttct tgatgaatca aagttgacgc
     8401 gagggatgat tgtacagagc gtttataaaa tagctggtgt ttttgctacg ggaatgatta
     8461 atgtaggaat tatgatgatt gatgatttcc ccgccccatg gtggaatgtt gcctataagc
     8521 cctaccgtat taaatactac aatgaccttt tgcagaatga aaaggataag ttcaatcgga
     8581 aaaagctgga agagatcatt gcaaagttaa aaaatttacc cgaggaaact gatacattgt
     8641 ttatttctga tgtatggctt catgatattt tagggtggca gaaacgattt ggttttgtat
     8701 attcaagctt tttgattttt aattataata aagatacaaa aggtgatgag ataggatttt
     8761 cagtacgtga tttttacctt tcacagaatg gtttaagcac cagaatggga ttggcagctc
     8821 ttgagaatgg ttttgaattg ggactgcatg gatataatca catgtcattg acactgacca
     8881 aacccatgta ttatgaatca acaccatggc cggataaaaa agctatggta gatgcactca
     8941 gcgtaacaaa gaatgaatgg atactattat atggcgaaag tgctctgcct tttagctatg
     9001 tagcaccgca caatataatt gataccatcg gtttacaggc gctgggtgag gtttttccca
     9061 gtatcagggt tgttgccact ctttacgtgg caaaaagtgg cgaatcagag caggaatttg
     9121 agtggtcacc tgacaa
//
LOCUS       DAOOJH010000096         9040 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6730_length_9040_cov_9.070562, whole genome shotgun sequence.
ACCESSION   DAOOJH010000096 DAOOJH010000000
VERSION     DAOOJH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6730_length_9040_cov_9.070562"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLJ75_10400"
     CDS             <1..282
                     /locus_tag="PLJ75_10400"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPD05663.1"
                     /translation="FLLLIAYYILEKNKTLIFTISMGLICAGAIGNILDRITGKPGVV
                     DFIYIGDDAVFRWPAFNVADSSIVIGATLLILHQIIEEKKRKSQKGHHQ"
     gene            complement(276..674)
                     /locus_tag="PLJ75_10405"
     CDS             complement(276..674)
                     /locus_tag="PLJ75_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05664.1"
                     /translation="MFRGHYCRVLVRCLFIIVLVIVSCGKTPPLQFCEGVTDEGTPVN
                     CGSQFTTGDMTLYVTAKEAFGSTTATVVIYEKKHYSQPEYTRFDYTINPDVSSTIIPL
                     SLYAEGTYIVTVIVGDKEIASGTISIVDTY"
     gene            complement(652..939)
                     /gene="xseB"
                     /locus_tag="PLJ75_10410"
     CDS             complement(652..939)
                     /gene="xseB"
                     /locus_tag="PLJ75_10410"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPD05665.1"
                     /translation="MEKKAKKPTFEEALEELETIAQKLESGELTLDESIQYYEKGVAL
                     AKFCHQKLEEAERKIEILQKQPDGSIQKKSIKVKEDTGEIEDDEDVQGSLL"
     gene            complement(923..2227)
                     /gene="xseA"
                     /locus_tag="PLJ75_10415"
     CDS             complement(923..2227)
                     /gene="xseA"
                     /locus_tag="PLJ75_10415"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPD05666.1"
                     /translation="MEDIEKHETVYSVSDITGIIKQLVASAPELNGVWIKGEISNLTY
                     HSSGHIYFSLKDEGAVIQAAFFKYSNKNLDFRLEEGMSVVVFGGVTVFEKRGQYQFIV
                     YKMRKEGLGELLQRIEKLKEKLYKEGLFDPRRKKPLPQLPRRIGIVTSPTGAAIRDII
                     KVAMRRFPNLEIVIAPAKVQGEGAAESIVAGIQELNKPVWGIDVIIAGRGGGSFEDLM
                     AFNEEKVVRAFAASKLPIISAVGHQIDSPLSDLAADVAATTPSAAAELAVPVKDDIQN
                     TIEQIIGKMTKRMMMVLQTFNNRIALALQYRAFQNPMSMLEQHELALSDIENRISLSI
                     HKRLYDARESLNRIKDLHLLVKNRLQNYKHVFMMNASKVEHLSPVAVLKRGYSITLKG
                     NTSVASVQGIRQGDEVKVIVYDGSMNCEVQSTSNEVNFGKKS"
     gene            complement(2238..3971)
                     /gene="pyk"
                     /locus_tag="PLJ75_10420"
     CDS             complement(2238..3971)
                     /gene="pyk"
                     /locus_tag="PLJ75_10420"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HPD05667.1"
                     /translation="MRRTKIVCTLGPTSEEKEVIVNLVRAGMNVARLNMSHSTHQMHE
                     KSIALIREIANELNTPIGILADLQGPKIRVGTLKEKVILEPGQQFILTTRDVPGNAIA
                     VSVSLKELPESVHKGQVILLDDGLLELVVDEVENTDIYTTVVRGGELKDHKGINLPNA
                     SIKTSSITQKDISDLEFVIKHDVDMVALSFVRTAQDVVQLRSIMEEFGGSIPIISKIE
                     KHEAVTNIDDIIAASYGIMVARGDLGIEIPIEQVPIVQKMIIEKCNQQAKPVITATQM
                     LDSMIRNPIPTRAEATDVANAVFDGTDALMLSGETAFGQYPVDAAATMAKIAQYAEDS
                     IMYKATMEERKIYTRAIITDAIALSASESAKTLGADCIITATQSGYTARKISKYKPQI
                     PVIAVTTNQKVVNQLSLSYGVVPVQIKHCEDINTLVDEALNTCKQKELIHNGDLLIIT
                     AGILAGVTGGTNVLRVYLVANEIARGIGYGDTVVKGYIRKKTVSGELSHNGIIITDDI
                     EQLKRSPLPLAVITSQSTIASPIIHFCREHGIPVIGGIAIDTLPCTDGDDVTLDILRG
                     TIYRGNVHLPF"
     gene            complement(4268..4459)
                     /locus_tag="PLJ75_10425"
     CDS             complement(4268..4459)
                     /locus_tag="PLJ75_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05668.1"
                     /translation="MLIATALSVWDSDSQYCLPSITIVLPFSGWCAVPDNNCKKAFVA
                     VLRITISALRFSDEHCCSL"
     gene            complement(4829..4902)
                     /locus_tag="PLJ75_10430"
     tRNA            complement(4829..4902)
                     /locus_tag="PLJ75_10430"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4866..4868),aa:Val,seq:cac)
     gene            5052..6314
                     /locus_tag="PLJ75_10435"
     CDS             5052..6314
                     /locus_tag="PLJ75_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05669.1"
                     /translation="MDTIAIKNFTLKRYIHVLLPLFIGSIIAYLDRVNIAYAALTMNQ
                     DLQFTPEIFGMGAGIFFIGYILFEIPGAIIAEKWNPKGWLMRIMITWGIVSFLMIFIT
                     RPWHFYTLRFLLGSAEASFYPVLYASVIPRWFTLQERPKAIALMLTSLQVSAIIGSPL
                     AGFLLDVSLFGLKGWQLLFVCEAIPALFFGILLYFILSNDPMKVSWLSDKEKEYLLNA
                     LSDESKQKTQHYTIWQALKDKEVLKLALIYFLWITGFWGFNYWMPTVIKELSGWSNIT
                     IGSLFAIPMTVSLIVMVYIGYSSSKTGEKRLHGAIPLAIAALGLGAGVFIQDPVVNML
                     FVCIAAIGIFGPFGVWWSYPTTFLSGAAAAGAVGLINSSGNIGGYVGPYLIGIIKNLT
                     NSFELSFLILAGMVLISSVMMLTLKKKA"
     gene            6317..6835
                     /locus_tag="PLJ75_10440"
     CDS             6317..6835
                     /locus_tag="PLJ75_10440"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPD05670.1"
                     /translation="MNGYVQCYTGNGKGKTTAALGLAVRAAGWSKHSIIIQFLKGKKS
                     GERIALKRFHDLITIETYGSKNFYIPGTDDEYFKELTRKAYDRCLAVCTDSSFNIVIC
                     DEILTALQWKLLSEEDIIHLVEIKHHETELILTGRGLSKTLFPYCDLITEMKEIKHYY
                     NNGVKARRGVEW"
     gene            complement(7050..9032)
                     /gene="acs"
                     /locus_tag="PLJ75_10445"
     CDS             complement(7050..9032)
                     /gene="acs"
                     /locus_tag="PLJ75_10445"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006892867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HPD05671.1"
                     /translation="MAEVTLKEIYPVPEKFRAKAYIKSRAEYEKIWKESLSNPEDFWG
                     KIAEEYVTWFKKWDKVMDCNYGTTSKDLWVRWFVNGKVNVSYNCLDRHLEKRGNQIAL
                     IWEGNEPTEDKKFTYKELHEQVCKFANVLKKNGVKKGDRVTFFLPMIPELAIGILACT
                     RIGAVHSVVFGGFSAEALRDRIQDCKSEVLISCDGTFRGAKAVPQKDTADKAIAECPT
                     VRLHIVVKRVGSKIQVNWNDKMDKWYEEEMANADANCPAEQMDAEDPLFILYTSGSTG
                     KPKGVMHTTGGYLTYVAYTHKMIFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATT
                     IMFEGVPTYPDFGRFWAIVEKYKVNIFYTAPTAIRALAKEGDSWVDKHDISSIQLLGS
                     VGEPLNPEAWNWYYHKIGRGECPIVDTWWQTETGGILITPLPGAIDIKPAMATVPFFG
                     VEPCIVDDNGTELEGVCTGNLCIKRAWPGQMRGVYGDPERFFNTYFTQFPGKYFTGDG
                     AKRDKDGYYQITGRVDDVINVSGHRMGTAEIESALVAHPKVAEAAVVGYPHEIKGQSI
                     YAFVTLNTGVEKSDELKKELVAHVRKEIGPIATPEMIQWADVLPKTRSGKIMRRILKK
                     IAAGEFDKSGMGDTSTLADPTVVDTLINDAKKLQGK"
BASE COUNT         2515 a         2014 c         1628 g         2883 t
ORIGIN      
        1 tttcttcttc ttatagccta ttatattctg gaaaagaata aaacgcttat ctttaccatc
       61 agcatgggac ttatctgcgc tggggctatc ggcaatatcc tggacagaat taccggcaaa
      121 cctggcgttg ttgattttat ctatattggt gatgatgctg tctttcgctg gcctgccttt
      181 aatgttgctg attcatcgat agtcataggt gctaccttgc ttatactgca tcaaattata
      241 gaagagaaaa agcgtaaatc gcaaaaaggt catcatcaat aagtatcaac aatgctgata
      301 gttcctgaag ctatctcttt gtcaccaact atcactgtaa caatataggt gccttctgca
      361 tatagcgaaa gcgggataat ggtgctggat acatcaggat tgattgtata atcaaatcgg
      421 gtatattcgg gctgactata gtgttttttt tcatatataa caacggtagc ggtggtgctg
      481 ccaaaagctt cctttgcagt aacatacaat gtcatatcac cggtggtaaa ctggctgccg
      541 cagttgaccg gcgtaccttc atcggtaacc ccttcacaaa attgcaatgg cggcgttttc
      601 ccacacgata ctatcaccag tactattatg aataaacacc gtaccaatac tctacaataa
      661 tgacccctga acatcctcat catcctctat ctctcccgtg tcttctttta cctttattga
      721 ttttttttgt atgcttccat cgggctgttt ttgcaaaatc tctatctttc gctcagcttc
      781 ttctaatttc tggtggcaaa attttgccag cgcaacacct ttttcataat actgtatgga
      841 ttcatcaagg gttaattcgc ccgattctaa cttttgggct attgtttcaa gctcctccag
      901 cgcctcttca aatgttggct ttttagcttt tttttccaaa attcacctcg tttgatgttg
      961 attgtacctc acagttcata gaaccatcgt atacaatgac ctttacctca tccccctgcc
     1021 gtatcccttg aactgaggct acactggtat tgcctttcag ggtaatggaa tagccgcgct
     1081 ttaaaaccgc caccggtgat aggtgctcaa ccttgcttgc attcatcata aatacatgct
     1141 tatagttttg caatctattt tttacaagca gatgcagatc ctttatcctg tttaatgatt
     1201 cccttgcatc gtacaggcgc ttatggattg acaatgaaat cctgttctca atatcagaca
     1261 gcgcaagctc atgctgctcc agcatactca tgggattttg aaacgcacga tattgcaatg
     1321 ccagcgcaat tcggttatta aatgtttgta ataccatcat cattcgcttg gtcatctttc
     1381 ctataatttg ttcgatagta ttctggatat catccttcac cggaacagca agctctgctg
     1441 ctgccgaagg ggttgttgcc gcaacatccg cagcaaggtc gctcagcggg ctatcaatct
     1501 gatgcccaac tgccgagatg atgggaagct ttgatgctgc aaacgcacgc acaaccttct
     1561 cttcattgaa tgccatcaaa tcttcaaatg aaccgccacc ccttcctgca atgataacgt
     1621 caatacccca cacgggtttg ttcaactcct gtatacctgc aacaatagat tcggcggctc
     1681 cctctccctg aaccttagcc ggagcaatga caatttcaag gttagggaag cgcctcatcg
     1741 caaccttgat aatatcgcgg attgcagctc ccgttggcga ggttacaatt ccaatgcgtc
     1801 tgggaagttg tggtaatggt tttttccgcc ggggatcaaa caatccttct ttatataatt
     1861 tttcctttag cttttcaatg cgctgcaaaa gctctccaag tccttccttt ctcattttat
     1921 atactataaa ttgatactgc cctcgctttt caaatactgt cacaccacca aaaacaacaa
     1981 cgctcatccc ttcttctaat ctaaagtcta aatttttatt ggaatattta aaaaaagctg
     2041 cctgaatcac tgcaccttca tccttcaacg aaaagtagat gtgccccgat gaatgataag
     2101 taagatttga aatctcacct tttatccata caccattgag ctcaggcgct gatgcaacca
     2161 gctgttttat aatgccggta atatcgctta ccgaatacac cgtttcatgt ttttcaatgt
     2221 cttccataca tttctactta aaagggtaaa tgaacattgc cacgatagat agtacctcgt
     2281 aaaatatcaa gcgtaacatc atcaccatca gtacatggta gcgtatctat agcaattcca
     2341 cctatcaccg ggatgccatg ttcccgacaa aaatgaataa tgggtgatgc tatagtactc
     2401 tggctggtaa taaccgctaa cggcaatggt gatcttttta actgctcaat atcatcggtt
     2461 atgatgatac cattgtgcga tagctctcct gatacagttt tttttcgtat atatcctttt
     2521 accacggtat caccatagcc aataccacga gctatttcat ttgcaacaag atacactcgc
     2581 aatacatttg tcccaccggt tactcccgct aaaatacccg cagttataat cagcaaatcc
     2641 ccattatgaa taagctcttt ttgtttacat gtattaagag cttcatcaac aagtgtattg
     2701 atatcttcac agtgtttaat ctgcaccgga acaactccat acgacaacga taactgattc
     2761 accacttttt ggtttgttgt taccgctata accggtatct gaggtttgta tttggatatc
     2821 tttcgagcag tataccctga ttgcgtggca gtaattatac aatctgctcc cagcgttttt
     2881 gctgattctg atgctgataa ggcaatggca tctgtaatga tcgcgcgtgt atatatcttt
     2941 ctttcttcca ttgtagcttt gtacataatg gagtcttcag catattgagc aatctttgcc
     3001 attgttgctg ctgcatcaac aggatactgt ccaaaagcag tttccccgga aagcatcaac
     3061 gcatctgtcc catcaaaaac agcatttgcc acatcagttg cttctgctcg tgttggtatg
     3121 ggattgcgta tcatggaatc cagcatctgg gtagcagtta ttaccggttt tgcctgttga
     3181 ttgcattttt caatgatcat cttctgaact atgggcacct gctctatggg gatttcaata
     3241 cccagatcgc cacgcgcaac catgatacca tacgatgcag caataatatc atctatattg
     3301 gtaactgctt catgcttttc aatctttgat ataatgggta ttgatcctcc aaattcttcc
     3361 ataatgcttc tgagttgaac aacatcctgt gcagtgcgca caaatgacaa ggcaaccata
     3421 tctacatcat gctttattac aaactccaga tcactgatat ccttttgagt tattgagctt
     3481 gtttttattg atgcattggg caaattaatc cctttatgat cttttagctc accaccacgg
     3541 acaactgtag tatagatatc agtgttttca acctcatcaa caaccagttc aagcaaacca
     3601 tcatcaagaa gtatgacctg ccctttatgt accgattcgg ggagttcttt taacgaaaca
     3661 ctaactgcta ttgcattgcc agggacatca cgcgttgtta agataaactg ctgtcctggc
     3721 tccaggatta ccttttcttt taatgtgcca actcgtattt ttggcccctg caaatcagcc
     3781 agtatcccga taggcgtgtt cagctcattt gctatttccc tgatcagtgc aatacttttt
     3841 tcatgcatct gatgggtgga atgtgacatg tttaaccttg caacattcat ccctgcacgg
     3901 acaaggttta caataacctc tttttcttca cttgttggtc ccagcgtaca tacaatcttt
     3961 gttcttcgca tagattctcc tgaaattttc atcctgagtg gaattgttct ttaaaggtac
     4021 tatcggtaac aaaacttgtt acaacatact gtgcttaaaa aacaatcctc acctgcttgt
     4081 tcatatttaa taaatatagc aaaaaatatt gaaaaaaatt atctgtttgt tattatagcc
     4141 ataatttttt tattaccagt ataagtatga gcagtttacc tttacagtgt ttctttgtac
     4201 aatacaataa aaattttgtg atccagcatc acgatgcata ccattttttc cttgatgctg
     4261 aagatgctca taagctgcaa cagtgctcat cactgaatcg caaagccgaa atagtaatac
     4321 gcaaaactgc cacaaaagcc tttttgcaat tgttatctgg aaccgcgcac catccactga
     4381 acggtaatac tatcgttatt gatggcaaac agtactggct atcactatcc cacactgaca
     4441 acgctgtcgc tatcagcata agcgaaaaac ctcatggcat tgacatcgaa tcaaccatac
     4501 ggcatgctgt ctatcgcaac aaaattgtta aacgttactt ttccataggc gaacaacagt
     4561 ttgtaaaccg ctcgttgttg cataaaacca aacggtttat ttcactatgg acaataaaag
     4621 aggcaattgc aaaattaaaa ggactatcgc ttgcacaggc tttacagtac gacaccatac
     4681 taaatgctga cggtttattt ttatccaatg ttacactatt gaaaaactat cactgcggac
     4741 tggcttttgg cgatagttcc tgtgataaac tttctgttac aatgtacaca gcagacgaaa
     4801 gggatatttt gcatgtattg tgtagatgtg ggcgataccg gattcgaacc agtgacctcc
     4861 tgcatgtgag gcaggcgctc taacctgctg agctaatcgc cctatactgc aatactgaaa
     4921 taatttttta aaattgcaag catttttatg aagtatacta ttttaaatat tttttacata
     4981 aaaacaattg caattttcat tatcacttca ttacaatata ctttgctaca aagatacagg
     5041 atgattgttc tatggatact atcgctataa aaaattttac cttgaaacga tatatacatg
     5101 ttttattacc tctatttatt ggctcaatca ttgcatacct ggatagggtt aatattgcct
     5161 atgcagcatt gacgatgaat caggatttac agtttacccc ggaaattttt gggatgggtg
     5221 ccggtatctt ttttataggc tacatactct ttgagatacc gggcgccatt atcgctgaaa
     5281 agtggaatcc caaaggctgg ctcatgcgta taatgataac gtggggtatt gtttcatttt
     5341 taatgatatt cattaccaga ccatggcatt tttatacatt gcgcttttta ttgggcagcg
     5401 ctgaagcaag cttttatccc gtactctatg ccagtgtcat cccacgctgg tttacattgc
     5461 aggagcgacc taaagctata gcattgatgc ttacctcgtt acaggtttct gctattattg
     5521 gctcaccact tgcaggcttt ttattagacg tatcactatt tggattaaaa ggatggcagc
     5581 tattatttgt atgtgaagct attccggcac ttttctttgg catactttta tactttatcc
     5641 tgtcaaatga tcccatgaag gtatcatggc tatctgataa agagaaggaa tatcttctca
     5701 atgcattatc agatgaaagt aaacaaaaaa cacagcacta tactatatgg caggcgttaa
     5761 aggacaaaga ggtattaaaa ttagcactca tctatttttt atggataacc ggattctggg
     5821 gatttaatta ctggatgcca accgttataa aggaactatc gggctggtct aatatcacta
     5881 ttggttcact ttttgccata cccatgacag tatcactcat tgtaatggtt tatattggct
     5941 attcttcgtc aaaaaccggc gaaaaaaggc tccatggtgc aataccgctt gctattgccg
     6001 ctctagggtt gggagcaggt gtatttatac aagatcccgt tgtcaacatg ctatttgtat
     6061 gcatagctgc cattggcata tttggcccat ttggggtctg gtggtcatat ccaacaacat
     6121 ttctttctgg tgcagccgca gcaggagctg tagggctcat taactcatcg ggtaatattg
     6181 gtggatatgt tggaccttat ctgataggta tcattaaaaa cttaaccaac tcttttgaac
     6241 tttcattttt aatccttgca ggtatggtgc tcatcagcag cgttatgatg ctcacattaa
     6301 agaagaaggc atgatcatga atgggtatgt acagtgttat accggcaacg gtaagggcaa
     6361 aacaaccgca gcattgggat tagctgtccg tgccgccggg tggagtaaac attcaatcat
     6421 catacaattc ttaaaaggaa aaaaatcggg tgagcgtatt gcactgaaac gattccatga
     6481 tcttatcacc attgaaactt atggaagcaa aaacttttat atacccggaa cagatgatga
     6541 gtattttaaa gaattaacca gaaaagctta tgatcgatgt ttagctgtat gtaccgatag
     6601 ttcctttaat atcgttatat gtgatgagat attaactgca ttgcagtgga aacttctttc
     6661 agaagaggat attatacatc tggttgaaat aaaacatcat gaaactgaat taattttaac
     6721 tggtcgcgga ctttccaaaa cattattccc ctactgtgat ttgattacag agatgaagga
     6781 gataaaacat tattataata atggcgttaa agctcgcaga ggggttgaat ggtgatggtg
     6841 tagtgttgag tcagtactcc agagtttttc ccccagttga attgacatca atgtattctc
     6901 cgtttttttt catggtattt ggtgcatggt actgaatatg attgaatcta ctgaatcgat
     6961 ttttttcgca tagttgatta ttaaaaaagg ctgccaatta cccggcagcc ttttttctac
     7021 ttctcttcta tttctattgt aaatatttct tacttgccct gcaatttttt tgcatcattg
     7081 ataagtgtat caacaacagt tgggtcagcc agcgttgaag tatcacccat acccgattta
     7141 tcaaattcgc cagcagcaat cttctttaag attcgtcgca tgatctttcc tgaacgagtt
     7201 tttggcagga catctgccca ttgtatcatt tcaggtgttg caataggtcc aatctcttta
     7261 cggacatgag caacaagctc cttcttaagt tcgtcactct tttctacacc agtatttaac
     7321 gttacaaacg cataaattga ctgtccctta atctcatgag gatatccaac aacagcagct
     7381 tcagctactt taggatgtgc aacaagagca gattcgattt cagcagtacc catacgatgc
     7441 cctgatacgt tgataacatc atcaacacgg cctgttattt gatagtatcc atctttgtct
     7501 cgtttagcgc catcacctgt aaagtactta ccaggaaatt gcgtaaagta tgtattaaaa
     7561 aatcgttctg gatctccata aactcctctc atctgccctg gccatgctct ctttatacaa
     7621 agatttccgg tgcatacacc ttcaagctct gtgccattat catcaacaat acatggctct
     7681 acaccaaaga atggtactgt tgccatagcc ggtttaatat caattgctcc tggtagcggg
     7741 gtaatcaaaa taccgccggt ttctgtctgc caccatgtat caactattgg acactcaccc
     7801 ctgcctatct tatggtaata ccagttccat gcctctggat taagaggttc accaacagaa
     7861 cctaatagct gaatggaaga aatatcatgt ttgtcaaccc agctatcacc ttcttttgct
     7921 aacgcacgga tagcagttgg agctgtataa aagatattta ctttatattt ctcaacaatt
     7981 gcccagaatc ttccaaaatc aggatatgtt ggaacacctt caaacataat ggttgttgca
     8041 ccatttgcca gtggcccata tacaatatat gagtgaccgg ttacccagcc aacatctgct
     8101 gtacaccagt agatatcgcc atcatgataa tcaaaaatca ttttgtgagt gtaagcaaca
     8161 taggttaaat atccaccggt ggtgtgcatt acacctttgg gtttccctgt ggagcctgat
     8221 gtataaagaa taaacagagg atcttctgca tccatctgtt ctgccgggca gttagcatct
     8281 gcgttagcca tctcttcttc ataccactta tccatcttat cattccagtt gacctgaatc
     8341 ttgctgccaa ccctcttcac aacaatgtgc aaacgaaccg ttgggcactc agcaattgct
     8401 ttatcagcag tatctttttg aggaacagct tttgctcctc ggaaagtacc atcacatgat
     8461 atcaacacct cagatttgca gtcctgaatt cggtcacgca gtgcttctgc agaaaatccg
     8521 ccaaaaacaa ctgagtgaac tgcaccaatt cgtgtacagg ctaaaatacc aattgcaagc
     8581 tctggaatca tggggaggaa gaatgtcaca cggtcacctt tctttacacc attcttcttt
     8641 aaaacattgg caaacttaca cacctgctca tgaagctctt tatacgtgaa cttcttgtct
     8701 tcggttggtt cattgccttc ccagatcaaa gcgatctgat taccccgttt ttcaaggtgt
     8761 ctgtccagac agttgtatga tacattaacc ttaccattta caaaccatcg tacccagagg
     8821 tcctttgaag ttgtaccata attgcaatcc ataaccttat cccatttttt gaaccaggtc
     8881 acgtattctt ctgcaatctt gccccaaaaa tcttctggat tggacaatga ctctttccag
     8941 atcttttcat actcagcccg gctctttata tacgcttttg cgcggaattt ctctggaacc
     9001 gggtaaatct cttttaatgt aacttctgcc atctattacc
//
LOCUS       DAOOJH010000097         8839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_6918_length_8839_cov_9.244763, whole genome shotgun sequence.
ACCESSION   DAOOJH010000097 DAOOJH010000000
VERSION     DAOOJH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6918_length_8839_cov_9.244763"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..252
                     /locus_tag="PLJ75_10450"
     CDS             97..252
                     /locus_tag="PLJ75_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05672.1"
                     /translation="MLSYPIDYCKERLSAYKVPKYVEFIDELPLTAIGKPDRKVLKQR
                     EMEKLKS"
     gene            complement(380..1108)
                     /locus_tag="PLJ75_10455"
     CDS             complement(380..1108)
                     /locus_tag="PLJ75_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05673.1"
                     /translation="MYKLILSLVCISFVFTGCTKAKIDEKNILGDWEAIKGDYQEVSF
                     YKEDGKSKFSAYLNGRLFTDGTWLIKGDSLILQLSTGETVTYTRVAVKNGILELNGGQ
                     QQYQPLRTDQQKIVELIAAVASIEGISFSKPADTQFPWNFDEGTSEKKVKGKMIKATI
                     QLTTDDYTDLANASRKVVELLTSKGFTSSDYNMTEIQSAAENGPLKVLIKQQAPISYD
                     PETDTPVSIKGQNAYLEIMCGVIE"
     gene            complement(1204..2547)
                     /locus_tag="PLJ75_10460"
     CDS             complement(1204..2547)
                     /locus_tag="PLJ75_10460"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPD05674.1"
                     /translation="MRLTLHLQMSLRAESFLEEAIAHKQTLALVTLATCPHTIPDNAG
                     RGRCVVRSTLLIFFIIVAMGCGGMKYKVVNTRHTVSTGFTVHSNIYFLCDYGLYQPGR
                     QIIPMYMYSPGDVYMHALYLYSYTPATNTLKQVLQIQSPVSTNIDISNAAWVEDNGIV
                     YCIYSAGWDNIQKKVQKDVLSFNINAMNVIALSNYEKQQILKKYGTRTNKNHMLSQTD
                     ILYYVDHLPMELWLLPSPLTFCTLDDADLKEVLVQGIGDSNFKEVIFAKLQQCSKKEL
                     LAVAAGIEKRYKSLESYQKMEYAPYRERWVTRILLAAFYGLSADKNLTLAQAVYHKNN
                     DIFYKLVNNENINYCDQHGTTLCMIAAYADNIEVLSYLLKANADMDAQDAFGCTALMY
                     AVFGRAAEAMGFLIKNNADTEVVSGSNCTAWVHIDTAGMRKRYVLLTEQKVQKKQ"
     gene            complement(2507..4288)
                     /gene="ade"
                     /locus_tag="PLJ75_10465"
     CDS             complement(2507..4288)
                     /gene="ade"
                     /locus_tag="PLJ75_10465"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPD05675.1"
                     /translation="MNFIPLETITMELADVAMGRRKADMVIKNGTLVNVNTKELQEHI
                     DVALYKGRIALVGDASHCIGETTQIIDATGLYIAPGFMDGHIHVESSMLTVSQYSTAV
                     VPHGTTAIFMDPHEIANVLGLKGVKLMLDEARDVPLRVYATVPSCVPAAPGLEDAGAE
                     IGPEEIAEAMKWDAIAGLGELMNFPGVFNSVPQVHKEIEVTLKAGKPVTGHFALPDTG
                     KMLNAYIASGVRSCHESVRHEDALAKMRLGMYAKIREGSAWHDLKEVIKAITHNTVQS
                     RFAILVSDDTHPDTLIKLGHLDHIVKRAIEEGVDPVTAIEMVTINTAECFLMSKDFGS
                     VSPSKVADIVLLSDLYSVTVKAVIIGGRLVARDGTMLSSAKKVTYPDWSKNTVNVGKT
                     LTPQDFMLTVNKSEVKVRVIQIEEAKVTTKQVIETLKTIDGNVAPNKEKDIAKAALFE
                     RHKATGTKGLGFVKGFGLKKGAVASTVAHDSHNLLIVGTDEEDMAFAGNELIKAGGGM
                     IAVADGKVLAIVELPVAGLLTDEPVEAVQKKVETLEKAWKMLGCNLVSPFMTMALLGL
                     PVIPELRLSNRGLVDAVNFTFTDVIAG"
     gene            complement(4301..4672)
                     /locus_tag="PLJ75_10470"
                     /pseudo
     CDS             complement(4301..4672)
                     /locus_tag="PLJ75_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014999.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF234 domain-containing protein"
     gene            complement(4752..6206)
                     /locus_tag="PLJ75_10475"
     CDS             complement(4752..6206)
                     /locus_tag="PLJ75_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968717.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPD05676.1"
                     /translation="MWSISLLTIFLALMVIIGIWGMRKTSTLGDFFLGGRTIGPWVSA
                     LAYGTSYFSAVLFIGFAGKLGWVYGLNAVWIGVGNALFGSLLAWLVLGRRTRIMTQNL
                     DVMTMPEFLMERFDGKYIKMFAAVIIFIFLIPYSASVFKGLGHLFEVNFNISYDLALI
                     IMIGITGIYLVLGGYFAITLTDFIQGIIMVAGALTMVLILSSKNGGIVQSIALVAEKY
                     PLHVPAGKQPGWILLSAFIFMTSFGTWGLPQMVQKFYAIKSQDLIKKAAIVTFCFATI
                     IGVCAYFTGSLSHLFYDAVPVVGNKPAFDLLIPDLLTKHLPEPLMAVILLLILSASMS
                     TLSSLVLVSSSAIAIDLYKGHVNPQITKENSVAMMRFLSALFVILSYFTARYEFGIII
                     TLMSLSWGAVAGAFMAPYLYGLFWKRTTLAGVYAGMITGLVVSIGLFFYLGAEKSAVA
                     SSLGMIIPFFIVPLVSVLTKKPDGALIQKAFEKI"
     gene            complement(7044..8750)
                     /locus_tag="PLJ75_10480"
     CDS             complement(7044..8750)
                     /locus_tag="PLJ75_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPD05677.1"
                     /translation="MLLFNPKKYERHHADDRSKQIMLKTIEFFEQKGLKKIKEDDQAA
                     VWYDDFLEFIKKEQTFATLLTPSGYGDKDSRWDMWRIEEYNEILGFYGLCYWYAWQVS
                     ILGLGPIWMGQNEEVKHKAAKLLKDGGIFGFGLSEKEHGADIYGTDMMLYPKGDGTYL
                     ARGDKYYIGNGNAAALLSVFGKMADTGEYVFFVVDTKHPNYECVKKISTSGVRQAYVA
                     EFALHDYPITEADILSRGNHAWDSSLNTVNVGKYELGWASIGICEHAFYEAIDHAANR
                     RLYNMYVTDFPHVKKIFTEAFARLAAMKLFALRACDYFRTANPDDRRYLLYNPIQKMK
                     VTMEGEKVIGLLHEVIAAKGFEQDTYFEMAIRDIGMLPKLEGTTHVNMALIVKFLQNF
                     MFKPKEYPEVPRRDDPANDSFLFNQGPAKGLGSIQFNDYRKAYEGIKSNNLEIFLEQI
                     EAFRNFLIKGTPDEKQTKNIDYMLSVGELFTLIPYTQLICENKKIYNIEDEIIDEIFK
                     FIVSDFSAYATRLYTGQKNSDKQNELIVKMIKKPVLDDAIFNKVWNEQVYALKGQYTM
                     NE"
BASE COUNT         2673 a         1968 c         1478 g         2720 t
ORIGIN      
        1 ctcaatgcac aaaaaggtat tgtcatatat ggaggagacc acatattcaa tcagggtgaa
       61 gctatggcag tagttccatg gtgggcattg tagagattgt taagttatcc aattgactac
      121 tgtaaagaaa ggctttcggc gtataaagta ccaaaatatg ttgaatttat tgatgaactt
      181 cctctaacgg caatagggaa accagaccgt aaggtactga aacagcgtga gatggaaaag
      241 ttaaaaagct aatcatgaaa taattttgtc atctatctta cagaagattt tatgataaaa
      301 agagtattcc ccaatgaagc aggggatact ctttactgtg aacgcatgat tttttcagca
      361 actataaaaa ataaagttat tattcaataa ccccgcacat tatctcaagg tatgcgtttt
      421 gccctttaat tgaaacaggt gtatcggttt cggggtcata gcttatgggt gcctgctgtt
      481 ttataaggac tttaagaggg ccattttctg cggcagattg aatttcggtc atattataat
      541 ctgatgaagt aaacccctta gatgtcagca actcaacaac tttccttgat gcatttgcca
      601 gatctgtata atcgtcagtt gttagctgta tggttgcctt tatcattttt cctttcacct
      661 ttttttcgct ggtaccttca tcaaagttcc atggaaattg tgtatcagcc ggttttgaaa
      721 agctaattcc ttctatagat gcaactgcag caatcagctc aactattttc tgttgatctg
      781 ttcggagggg ttgatattgc tgctggcccc catttaattc cagaatgcca tttttaacag
      841 caacccgtgt ataggttacc gtttcaccgg tactcaattg cagtatcagc gagtcgcctt
      901 ttatcaacca tgtcccgtca gtgaacagtc ttccattaag ataagctgaa aatttacttt
      961 taccatcttc cttgtaaaaa cttacctcct gataatcccc ctttattgct tcccaatcgc
     1021 ccaatatatt cttttcatca attttcgcct ttgtacatcc tgtaaacaca aaagaaatac
     1081 aaacaaggct tagaattagt ttatacatac cagtacctcc atatagtatt taaaaatcgt
     1141 gcattcacat agttagtgca tcgtgatgct gtgatatgtg acatcaataa ccatataatc
     1201 ctgctattgc ttcttctgta ctttctgctc tgttaacagt acgtaccgct tgcgcatgcc
     1261 tgctgtatcg atgtgcaccc atgcagtaca attgcttccg gaaacaactt ccgtatcagc
     1321 attatttttt attaaaaacc ccatggcttc ggcagcacgc ccaaaaactg catacatcag
     1381 agccgtacac ccaaatgcat cctgtgcatc catgtctgca tttgctttaa gtaaatatga
     1441 cagcacctca atattgtcag catacgcagc aatcatacac agggttgtcc cgtgttgatc
     1501 gcagtaattg atattttcgt tatttactaa tttatagaat atatcattgt ttttatgata
     1561 tactgcctgc gccagtgtta aatttttgtc agcacttaac ccatagaacg cagcaagcaa
     1621 tattcgcgta acccatcgct ccctgtatgg agcatattcc atcttttgat agctttcaag
     1681 gcttttgtat cttttctcaa taccggcagc tactgcaagt aattcttttt ttgaacactg
     1741 ttgtaattta gcaaatatga cttccttaaa gttactgtcg ccaataccct gaacaagaac
     1801 ctcctttaaa tcggcatcat caagtgtgca aaatgtaagc ggcgaaggca gcaaccacag
     1861 ctccattggc aaatgatcca cataatacag aatgtcagtt tgggacagca tgtgattttt
     1921 atttgtgcgt gtaccatatt ttttcaggat ctgctgtttt tcatagttgg agagagcaat
     1981 gacattcatt gcattgatat taaaagacaa tacatctttc tgcacttttt tctgtatatt
     2041 gtcccagcca gcagaataaa tgcagtacac aataccgtta tcctccaccc atgcggcatt
     2101 gctaatatca atgttggtgc tgaccggtga ttgtatctgt aacacttgtt taagcgtatt
     2161 agtggcagga gtatatgaat acagatacag tgcatgcata tacacatcac cggggctgta
     2221 catgtacata ggtattatct gcctaccggg ctgatacaaa ccgtaatcgc agagaaaata
     2281 aatattgcta tgtacggtaa agcctgttga taccgtgtgc cttgtattaa caaccttgta
     2341 tttcataccc ccgcaaccca tagcaacgat aataaaaaaa attaatagtg tgcttctcac
     2401 aacacacctc cctctcccgg cgttgtctgg tatcgtatgc gggcaggttg caagggttac
     2461 cagtgccagt gtttgtttat gggcgatagc ttcctctaaa aagctttcag cccgcaatga
     2521 catctgtaaa tgtaaagtta accgcatcaa caaggccccg gttactgagc ctgagctccg
     2581 gaataaccgg taatcctaaa agagccattg tcataaacgg tgaaaccaga ttgcatccta
     2641 acattttcca ggctttttct aaagtttcaa cctttttttg aactgcttca accggttcat
     2701 ctgtcagcaa ccctgcaaca ggaagctcca caatagccag cactttgcca tcagcaacag
     2761 ctatcatccc accaccagct ttgataagct cattcccggc aaaagccata tcctcttcat
     2821 cggtaccaac gataagcaaa ttatgcgaat catgtgcaac agttgatgca acagcgcctt
     2881 tttttaagcc aaaacctttt acaaaaccca gcccttttgt tccggtggct ttatgacgct
     2941 caaacagcgc agcctttgca atatcctttt ctttattggg tgcaacattg ccatcaatag
     3001 tcttcaatgt ttcaatgacc tgctttgttg taacttttgc ctcttctatc tggattacac
     3061 gaactttaac ctcggattta ttgactgtta gcataaaatc ctgaggggtt aaggttttgc
     3121 caacattaac ggtgtttttt gaccagtcag gataggttac cttttttgcc gatgaaagca
     3181 ttgttccatc cctggcaacc agcctgccgc caataataac tgctttcaca gtaacactat
     3241 aaagatccga aagaagaaca atatcagcaa cctttgatgg cgatacactt ccaaaatcct
     3301 tgctcatcaa aaagcattcg gcagtattaa tggttaccat ctctatagca gttacagggt
     3361 caacaccttc ttcaattgct cttttaacaa tatgatcaag atgtcccagt tttatcaacg
     3421 tatcaggatg ggtatcgtca ctcacaagga tagcaaaacg gctttgcact gtattatggg
     3481 taatagcttt tattacctct tttaaatcgt gccatgctga gccttcacgt atctttgcat
     3541 acattcccag acgcatcttt gccaatgcat cctcatgcct caccgattca tgacatgaac
     3601 gcacgccaga agctatgtat gcattgagca tttttcccgt atcgggcaaa gcaaagtgac
     3661 ctgttaccgg tttgccagct tttagtgtta cttctatctc tttatgaacc tgaggtacgc
     3721 tgttgaatac accgggaaaa ttcataagct cacccaaccc tgcaatggca tcccacttca
     3781 ttgcttctgc tatctcttca gggccaattt cagcacctgc atcctccagc cctggtgcag
     3841 ccggcacgca tgaaggcact gtagcataaa ccctgagcgg cacatcccgt gcttcatcaa
     3901 gcatcagttt cacaccttta agccccagca cattagctat ctcatgcggg tccataaaga
     3961 ttgccgttgt tccatgtggt accactgcag tgctgtactg tgataccgtc agcatgctgc
     4021 tttcaacatg aatatgccca tccataaatc caggtgcaat atataaaccg gtagcatcaa
     4081 ttatttgcgt tgtttcgcca atacagtggc tggcatctcc taccaatgct attcttcctt
     4141 tatatagggc gacatcgata tgttcctgca actcttttgt attgacattc accaatgttc
     4201 catttttaat taccatgtca gccttacggc gtcccatggc aacatcggca agctccatgg
     4261 taatcgtttc aagtggtata aagttcataa agttatcccc ctattgaatt tgttgcacta
     4321 tagtattgca atcaaagagc ataccatcat ctttttttgc aatcattttc atgtcgtttg
     4381 taaatccggt tttactaaaa agcatatagc agttattttg atctttgcaa tagacaatgt
     4441 cccttgatat ttttactaaa atcaggatat atagtttttt attgctccat gtgcactcac
     4501 caaaccacac cgtatcagta tcatcatcca gagcaacaat gtcaatctca ttatcttttg
     4561 accaccacct gcctatacag ttacattgca gtaattgttt ttgcatcaga gcatggcata
     4621 gttctaaaca ggcgtcttca aaggttaaag aaacatgatg ctcaacatta tccttaaatt
     4681 ttttaacaca atatcaggtt gttcacgttc aatccatcgc ctattgggaa atataaaatg
     4741 aaaacaagtt atcaaatttt ttcaaacgct ttctgaatta aagccccatc aggctttttc
     4801 gttaatacgc tcaccagtgg tacaataaaa aatgggatga tcattcctaa actggacgca
     4861 acggcagact tttcagcacc cagataaaaa aacaaaccaa tagacaccac aagccctgta
     4921 atcataccag catatacccc ggcaagggtt gttcgtttcc agaacagtcc atacaaatac
     4981 ggtgccatga atgcgcctgc aacggctccc catgaaagcg acatcagggt tataattatt
     5041 ccaaattcat atcgtgcagt aaaatatgat aatatgacaa acaaggcact caaaaagcgc
     5101 atcattgcta cagaattctc ttttgtaatt tgtggattaa catgcccttt gtaaaggtca
     5161 attgcaatag cagatgaaga tactaataca agtgatgaaa gagtagacat ggatgcagaa
     5221 agaattaaca gtaatatcac agccattaaa ggctccggca gatgttttgt cagtaaatca
     5281 gggatgagca ggtcaaatgc cggtttatta ccaacaacag gaactgcatc ataaaacagg
     5341 tgtgatagcg aaccggtgaa atacgcgcaa acaccaataa ttgttgcaaa acaaaatgtt
     5401 acaatagcgg ctttctttat aagatcctga cttttaatag cataaaattt ttgcaccatc
     5461 tgcggcaagc cccatgtacc aaatgatgtc atgaagataa atgcagaaag gaggatccat
     5521 cctggttgtt ttccagcagg cacatgtaat ggatattttt cagcaaccag tgcaatactt
     5581 tgaactatcc caccattttt agatgatagt atcagtacca tggtgagagc tcctgcaacc
     5641 ataataatac cttgaataaa atcggtcaat gtaatagcaa aatacccacc cagcacaagg
     5701 taaataccgg tgataccaat cattatgatg agtgctaaat catacgaaat attgaaatta
     5761 acttcaaaaa gatgtcccag acctttaaaa acagatgcag agtacggaat taaaaatata
     5821 aatataatga cagcagcaaa catcttaata tatttgccat caaagcgttc cattaaaaac
     5881 tcaggcatgg tcatgacatc taaattttga gtcataattc tcgtacgtct tcccagcaca
     5941 agccatgcta acagtgaacc aaacagcgca ttaccaacgc caatccagac agcattaagc
     6001 ccatataccc agcccaattt gccggcaaag cctataaaaa gcactgctga aaaatacgag
     6061 gtgccatacg ccaacgctga aacccatggg ccaatagttc ggccacctaa aaagaagtcc
     6121 cccagtgttg atgttttacg cataccccag ataccaataa taaccattaa agccagaaag
     6181 attgttaaca atgaaattga ccacatagct accgatctcc ttttttattt ttaatcgatc
     6241 atcatgcctg gcaattacat tgccaatgga atgttgtact aaaattactt tgaatttata
     6301 tttctatcaa gaaagcaggt tgtcaataat aaaataattt tgatgaagga tctatattgc
     6361 aatgaggaat agtgactcta taaacaaatc aatctccaga gggagtataa taactagaga
     6421 gaataaatat aaatacaagt gaaagcatta tccttttatc aattgatttc tgtatattat
     6481 cactcatatc aagaatataa ttactacgaa atagagtaaa ctctctggtc aggctcgcta
     6541 aaacatccct gtctttgact attaaaaacc tcctgtttgg aaacgaatgg agtatagaat
     6601 catctttttt tctcatcata cacatacatt tttggagaga aatatcctct tactggaata
     6661 aaaaatgtat ctctatcaag aacttcattt atctgatcca tatttcttct tccaataacc
     6721 agcaatgctt actatttaat ctgcatgcat ttttttaagt aattttttgc actgtaatga
     6781 ttattttttt cgataacaat atttttttat atttgtgata tgtttcaaat ttgaagttac
     6841 aaccaatacc cggcaccaga acattgccat ataaaatagc tatagtatta tttcagcaag
     6901 gaaacttaac ccttcactat tttgaagatt tttatgattt ttttattaca gatgattgta
     6961 taataatggg tgagggtttg atattccata acaaaattta aaaggacacc atgttctggt
     7021 gtccttatat tcatagtaaa cttttactca ttcatggtat attgcccttt caatgcatac
     7081 acctgttcat tccatacctt attaaaaatt gcatcatcca gaacaggttt tttgatcatc
     7141 tttacaataa gctcattttg cttatcgctg tttttctgcc ctgtgtaaag ccgtgttgca
     7201 tatgcagaga aatcgctgac tatgaatttg aaaatctcat caatgatctc atcttcaata
     7261 ttgtaaatct tcttattttc gcagataagc tgtgtatagg gaatcagtgt aaacagctcg
     7321 cccaccgata gcatataatc aatattcttt gtttgctttt catccggagt ccccttaata
     7381 aggaagtttc taaatgcttc tatctgttct aagaaaatct ccagattgtt gctcttgatg
     7441 ccttcatatg cttttctgta atcattaaat tgtatcgatc ccaatccctt tgccggtccc
     7501 tgattaaaca aaaagctatc attagcagga tcatcccggc gcggcacctc cggatattct
     7561 tttggcttaa acataaagtt ttgtaaaaat tttacaatga gtgccatatt aacatgcgtt
     7621 gtgccttcca gtttgggcag catcccgata tctcgaattg ccatctcaaa ataggtatcc
     7681 tgctcaaagc cttttgcagc tatgacctcg tgcagcaacc caattacctt ttccccctcc
     7741 atggttacct tcatcttctg aattggatta tacaaaaggt atctgcgatc atcaggattg
     7801 gcggtacgga aataatcaca agctcgcaaa gcaaacagct tcatcgccgc caaccgtgca
     7861 aaagcctcag taaatatttt cttaacatgc gggaaatcgg ttacatacat attataaagt
     7921 cttctgtttg ctgcatggtc gatagcttca taaaatgcat gctcacatat cccaattgaa
     7981 gcccacccta actcatactt gccgacattt actgtgttaa gcgatgaatc ccatgcatgg
     8041 ttcccacgtg ataagatatc cgcttcggta ataggataat catggagggc aaactcagca
     8101 acgtacgcct ggcgtactcc agaggtactt atctttttaa cacattcata attaggatgt
     8161 tttgtgtcca ccacaaagaa tacatattca ccggtatcag ccattttgcc aaataccgaa
     8221 agaagcgcag cagcattccc attcccaata taatatttat caccacgcgc taaataggta
     8281 ccatcacctt tgggatacaa catcatatcg gtgccataga tatcagcacc atgctctttt
     8341 tcagaaagcc caaacccaaa tatgccacca tctttcaata attttgctgc cttgtgtttt
     8401 acctcttcat tctgccccat ccagattggg cccaaaccca atatagatac ctgccatgca
     8461 taccaatagc atagtccata aaaacctaaa atttcattat actcctcaat gcgccacata
     8521 tcccaccgcg aatccttatc accataccct gaaggcgtaa ggagtgttgc aaaggtctgt
     8581 tcttttttta tgaactctaa gaaatcatca taccatacgg ctgcttgatc gtcttctttg
     8641 atcttcttca atcctttttg ttcaaagaat tcaatagttt tcagcataat ctgctttgat
     8701 ctatcatcag catgatgacg ttcatacttt tttggattaa atagtaacat tgttaccccc
     8761 tctccacttg taatatataa tacaaattag tatatactat ttatgattct tattgtttaa
     8821 tatagcacta catcaaaaa
//
LOCUS       DAOOJH010000098         8757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7005_length_8757_cov_9.430476, whole genome shotgun sequence.
ACCESSION   DAOOJH010000098 DAOOJH010000000
VERSION     DAOOJH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8757
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7005_length_8757_cov_9.430476"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1672)
                     /locus_tag="PLJ75_10485"
     CDS             complement(107..1672)
                     /locus_tag="PLJ75_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05678.1"
                     /translation="MYIVLRRSVMIVFVIIIMPALVYANFADTYGFGAQGVARGNAMT
                     AVVNDWSSVYYNIAGLGRTQGYLVLTVQQTEPKQQITLKQKGGKDKKEQEVVTVEPQV
                     ASPYDLYLYKDQLVINYMYTQPDMTIDIPRETVADKDLDFGTVALGLVLDLNHFVKLP
                     SFISSGRFGLGMGLLQDGTLVRVYDVDLRTHDFLRYGKEAERTVILAGMGFGFADDLF
                     GIGFGINAWSGGKGAVRLMDVDMASPNPQTPNSEIQMELSPKVAPVCGLYISPGKKIN
                     ALRGLEMGVAYRGEIYMEVKPFSTTAELIDGTIQLQMALNIFDYYTPHIISGGIAYTF
                     LPIKPLSGLTLSFDVEYQMWSKYKVSKAKEEYWSQYGIIMPEYDDIIVPKVGISYNFG
                     CLIPKMEWLTLHLGYMYRPTYVPDDTVNDASLFNFMDCNTHIASAGLTFTIPRMGPMV
                     APLVITVAGQMQMLQERKVKKDRVAIEQIYENFDGNPTNDDISQAEIDELYPDYSFSG
                     TVYTGFVEVALRW"
     assembly_gap    1772..1871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1872..5749)
                     /locus_tag="PLJ75_10490"
     CDS             complement(<1872..5749)
                     /locus_tag="PLJ75_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05679.1"
                     /translation="MKPFKSLFIIMCVATVLLSCSDSSKFLFQRNDPEPEYRLFDLLD
                     PYPALYDAFDTIDQHRFNVMLSDAINADVTGTKNALQQMPAINPDPLVDIVAALRIII
                     ERIINQDNYKWRSDPYDYDNYVTDFYSFLDDLDAASPGTTNELVSILRKSIGYIQYAH
                     GHEIETVMADLIDFLKDNHGQNVGTVLPLLQEGLGKTLLRANTFYDDSSNKLGNAVVG
                     MDALLSGINDIATEDADAREALYDVIRELGAVMTAKAGDKTFADILKELMINIEDYAT
                     VGGSVYSSNASYYHDGSDYYVNTELKNGVRQMLPGLALLFIRAGDNAQASPIYDPVDN
                     NSPNPDGRSALEYLTQKLYDLKLNCGIDFTNYEVEASLKRMTQYNGFGELRSSASYKV
                     SYLDHLLYTLIASYDFGFLTKTSDGASEPYNNDQDGHCSVRKHGEPTAGIITINDSLY
                     SMVSHAKEARSSSQNCTIGWLGAYNLALDARAVTQTWDGDTGGLLTSVEITNGAQGNY
                     IYRCKNSFLSSQANNYKFYLGYDFPTLLLFSGFSAGDAGIPNGGITGIVPTSNTTTVG
                     TNNDYRTYYPYVGNGLGELNTGRWTMGWIARACWEGEGPYYYADPNAKTRTASDGKVY
                     HVYFRPDGRIYAFVCKEDPANPVYFYPNDGGNDADETDNPQSLTIDGVEFKLRANRYR
                     ASWHTDYYLLKSDYINYHEDANHPPAGWTGYYSPANVVGTTGINKFKMHSLQDTKNKG
                     EYGDDLDASTNNAGCLWFWEKIAENNTVRGCNSHEEAMYRNFQWLMLEKKFVFIMPMR
                     SYVNIHATAAWCIGVTLNIDSPLFTIIEANGMAGIATAKKTGTVGTWVKLGNRGDGEG
                     CTNYGHNQPNGANYGDSLQPADGRLWVIVKEDSSYKTSLSVLTDGDYVDINTIWSTIL
                     GNGNVFPNIVGDNLAPIARMAFVQPDLVASNSSDIGNINAAAWQNRNKLLPIIVALSG
                     DLHEKSHYDKPSDQNNHWYNFSGNHKYPLKHLRDLIAMLGNPIMRYYKTPYTGAAKGW
                     FVPQIQDQHGRGVYAMFTPKPIDSAVNFNPNSSLRSVAAVLTENSQAAADGIIPALAN
                     SKMVTKLMALLQQIGQYGYGALPIYQDGTKGSSDYADWGARRKIFYGLEQIVTRMKCN
                     KSIEQTRDYYGISYPAWMFVKNSDDIDMDVALDELIGYDDVDGNPYVWGKGLAAFVDH
                     RDPAHPNYKGWNWNNYYKLVNGLGELMSNQGATNGYYCINDDLIAMIDKTLTSFKATN
                     AQLRGVRHTLGTVLYYYYDDTSQWIIPNEL"
     gene            complement(5771..5932)
                     /locus_tag="PLJ75_10495"
     CDS             complement(5771..5932)
                     /locus_tag="PLJ75_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05680.1"
                     /translation="MYLIHKVLSKMGLKKKSFLLGKIVLGSRFFVEIYRHASTVNPYY
                     RVTIAYMIF"
     gene            6983..8191
                     /locus_tag="PLJ75_10500"
     CDS             6983..8191
                     /locus_tag="PLJ75_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD05681.1"
                     /translation="MDLFDKCLAFTRVEEVKSFGLYPYYHPITKTEGPVVYMNNKRMI
                     MVGSNNYLGLTHDPRVKQAAIDAIEKYGTGCSGSRLINGSLDIHCKLEEALAQFVGKE
                     AACIFSTGYQTNQGAIVSLINKGEYIISDEENHASIIQGTLIAKALCGDSILVKFHHN
                     DMVDLENKISALPHDAGKLIVVDGVFSMNGHIAPLPDIVRIARKYNARIMTDDAHALG
                     VIGHDGRGTASYFGLTDQTDIIMGTFSKSLASSGGFLASSERVINYIKHNAPAIIFNA
                     SMPASNAAAALKALEIIQNEPESIQKLHNNAKRLRNGLEHLGFNVIKGETPIIPVIIG
                     DDIMTFTLWKKLFDYGVYTNPIIFPATPQGQQLIRCSVMATHTDEHIDYVLGMFEKAG
                     AELKLLHAKV"
     gene            complement(8312..>8757)
                     /locus_tag="PLJ75_10505"
     CDS             complement(8312..>8757)
                     /locus_tag="PLJ75_10505"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05682.1"
                     /translation="IAYCIMDNHIHIVIHTTSGGAPIARIVQYIKARFAEMYNKITGR
                     TGPFWNERYKDIIVQFAHDAFHYLLWLLWYIAFNPVRKRRCDSPTKYRYSSIQSYVQE
                     GADVGVPIDHHEYFLQLGDSFSERVRRFLQYEEAYRKRYSIAQWV"
BASE COUNT         2633 a         1863 c         1429 g         2732 t
ORIGIN      
        1 tagataaaaa aaggcgaaca ttaaataatt atgttcgcca gagattatat tttatagata
       61 tggggcgctt tatagattat aaagccaata atttatagtg tatatattac cagcgtaatg
      121 ctacctccac aaatccagtg tatacagtac cactaaatga gtaatcagga tataattcat
      181 caatttcagc ctgagagata tcgtcatttg taggattccc atcaaaattt tcatatattt
      241 gttcaatcgc caccctatcc ttcttcacct ttctttcttg tagcatctgc atctgtccgg
      301 caacagttat tactaaaggg gcaaccatag gtcccattct tgggatagta aatgttaacc
      361 ctgccgaagc aatatgggta ttgcaatcca taaaattgaa taacgacgcg tcattcactg
      421 tatcgtcagg aacatacgtg ggtcggtaca tatatcctaa atgtaaagta agccactcca
      481 tttttggtat taaacatcca aaattatatg atataccaac tttgggaact atgatatcat
      541 catattcagg cattattata ccatattgac tccagtactc ttctttagct ttggaaacct
      601 tatatttaga ccacatctga tattccacat caaacgataa tgttaaccct gataatggtt
      661 ttataggcaa aaaagtatag gcaattccac cactgataat atgcggtgta tagtaatcaa
      721 atatgttgag cgccatctgt aactgtatcg ttccatctat caactctgct gttgttgaaa
      781 atggtttaac ctccatatat atctcacctc tatacgctac tcccatctcc agccctcgta
      841 aagcattgat ctttttgccc ggggatatat ataatccaca aactggagca acttttggcg
      901 aaagttccat ctgtatttct gaattgggcg tttgaggatt tggcgatgcc atgtcaacat
      961 ccatcaagcg cactgcacct ttgccacctg accatgcatt gataccaaat cctattccaa
     1021 aaagatcatc cgcaaatcca aaacccatcc ctgctaatat gactgttcgt tcagcttcct
     1081 tgccatagcg taaaaagtca tgcgtgcgca agtcaacatc atatactctc acaagggtcc
     1141 catcttgcaa aagtcccata cccaatccaa aacgccctga tgaaataaac gatggcagtt
     1201 taacaaaatg atttaaatcc agtaccaaac ctaatgcaac cgtcccaaaa tcaagatctt
     1261 tatcagctac cgtctcacgg ggaatatcaa tggtcatatc aggctgtgta tacatataat
     1321 tgattacaag ctgatccttg tataggtaaa gatcatatgg tgatgccacc tgaggttcaa
     1381 cagtgaccac ctcctgttct tttttatctt tcccaccctt ttgttttaaa gtaatctgct
     1441 gttttggctc tgtttgttgt acagtcaata ccagatatcc ttgcgttctt cctaatcctg
     1501 ctatattata atatacactt gaccagtcat ttaccactgc tgtcattgca tttcctctgg
     1561 ctacaccctg cgctccaaat ccataggtat cggcaaaatt agcatacact aaagcaggca
     1621 taattataat gacaaatact atcatcacac tacgtctcaa cactatatac atcatgacct
     1681 ccgataataa actatatatc ttgtacaacc acaggactaa ccctatggtt gttgctgtgc
     1741 tctatcattt agtcaaaagc tgtcccaatc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn ntttagttca ttgggaataa tccactgtga tgtatcatca taatagtagt
     1921 ataataccgt acccaatgta tgacgcactc ctctcaactg ggcatttgtt gctttgaagc
     1981 ttgtcagcgt tttatcaatc attgctataa ggtcatcgtt tatacaataa tatccgtttg
     2041 ttgcaccctg attgctcatc aactcaccaa gaccattgac aagcttatag taattattcc
     2101 aattccatcc tttatagttg ggatgggctg gatctctgtg atcgacaaat gctgccagtc
     2161 ccttgcccca tacataggga ttaccatcca catcatcgta tccaataagc tcatccagtg
     2221 ctacatccat gtcaatatca tcactatttt taacaaacat ccatgcagga tagctgattc
     2281 cataatagtc tcttgtttgc tcaatactct tattgcattt catgcgagtt actatctgct
     2341 ccagaccata aaatatctta cgacgggcac cccagtcagc atagtcactg cttccttttg
     2401 taccatcctg atagattggt agtgcaccat aaccatattg accaatttgt tgtaacaacg
     2461 ccatgagctt tgtaaccatt ttactgtttg ccaaagccgg gataatccca tccgcagcag
     2521 cttgagaatt ttcggtcaac acagcagcaa ccgagcgtaa tgagctgttg ggattaaagt
     2581 ttacagcact atcaataggt tttggcgtaa acattgcata cacgcccctg ccatgttgat
     2641 cctgaatctg tggcacaaac catccctttg ccgcaccggt atatggcgtt ttataatagc
     2701 gcataattgg attgcccagc atggctatga ggtcacgcag atgttttagt ggatatttat
     2761 ggttaccgct aaagttatac cagtggttat tttggtctga tggtttatca taatgagatt
     2821 tttcatgtaa atcccctgat aatgccacaa taatgggcag cagtttatta cggttctgcc
     2881 atgcagcagc atttatatta ccaatgtctg atgaattgga agccactaaa tcaggttgta
     2941 caaaagccat tcgtgcaata ggagccaggt tatcaccaac aatattagga aaaacattac
     3001 cattaccaag gatagtactc catattgtat taatatcaac ataatctcca tcggttagta
     3061 cactcaaact agttttatat gatgaatcct ctttaacaat aacccatagc ctgccatctg
     3121 caggttgcag cgaatcacca taatttgcac catttggctg gttgtggcca taatttgtac
     3181 atccctctcc gtcaccccgg tttccaagct ttacccatgt accaactgtg ccagtttttt
     3241 tagctgttgc aataccagcc attccattag cttcaattat tgtaaaaagc ggagaatcta
     3301 tattcaatgt aacacctata caccacgcag cagtagcatg aatgttaaca tatgatcgca
     3361 taggcattat aaatacaaat ttcttttcca gcataagcca ttgaaaattt ctatacatgg
     3421 cttcttcatg actgttacat cctcttacag tattattttc agcaatcttc tcccagaacc
     3481 acaaacaccc agcattattt gttgaagcat caaggtcatc accatactca cctttgtttt
     3541 ttgtatcctg taatgagtgc attttaaatt tattgatacc agtagtaccc actacatttg
     3601 caggtgaata ataacccgtc catcctgcag gtggatgatt agcatcttca tggtagttta
     3661 tataatccga tttcagcaaa taataatcag tatgccagct tgcacggtat ctgttagctc
     3721 tgagtttaaa ttcaacacca tcaattgtta atgattgagg gttatcagtt tcatctgcat
     3781 cattgccacc atcatttgga taaaaatata ctggattagc aggatcttct ttacatacaa
     3841 acgcataaat cctcccatca ggtctaaaat atacatgata taccttacca tctgatgctg
     3901 ttctggtttt tgcgttggga tcagcataat agtagggacc ttcaccttcc cagcatgcac
     3961 gggcaatcca gcccatagtc cagcgtccgg tgttaagctc acccaatcca ttgccaacat
     4021 acgggtaata agtgcggtaa tcattatttg tgcctactgt tgtagtattt gatgttggaa
     4081 ctataccggt aattcccccg tttggaatac cagcatcacc agcagaaaaa ccactgaata
     4141 atagaagagt tggaaaatca taccctaaat aaaatttata gttattagcc tgtgaactta
     4201 aaaatgagtt tttacaacga tatatataat tcccttgcgc accatttgtt atttcaactg
     4261 atgttaatag acctcctgta tcgccatccc atgtttgcgt aacagccctt gcatccagag
     4321 ccaaattata agcacctaac catccaatgg tacaattttg gctggagctt cttgcttctt
     4381 ttgcatggct taccatggaa tataaactat catttatagt aataatacca gctgttggtt
     4441 caccatgttt gcgaacagag caatgaccat cctgatcatt attgtaaggt tctgaagcac
     4501 catcagaagt tttggttaaa aaaccaaagt catacgacgc tatcaaagta tacaacagat
     4561 gatctaaata cgatacttta tagcttgcac tgcttcggag ctctccaaat ccattatact
     4621 gggtcatcct tttcagggat gcctcaacct cataatttgt aaaatctatt ccgcagttta
     4681 acttcaaatc atacaatttc tgtgttaaat attccaatgc tgagcgcccg tcgggattgg
     4741 gcgaattgtt atccaccggg tcatagatag gagatgcttg tgcattatcg cctgcccgta
     4801 taaaaagcaa cgccaaacca ggcaacatct gtcgtacacc atttttaagt tcagtattaa
     4861 cataataatc gcttccatca tgataataac ttgcattgga gctataaaca ctgcccccaa
     4921 ctgtagcata atcctcaata tttatcataa gctctttgag tatgtcggca aatgttttat
     4981 ctcccgcttt tgccgtcatt accgcaccca gctcgcgtat tacatcatag agcgcttcac
     5041 gtgcatcagc atcctccgta gctatatcgt ttatccctga cagcagcgca tccataccaa
     5101 caactgcatt tcccaattta ttgctgctat cgtcataaaa agtatttgcc cgtaataatg
     5161 ttttacccaa tccttcctgc aacagtggca acaccgtgcc aacattctgc ccatggttat
     5221 cctttaaaaa atcaatcaaa tctgccataa ctgtctctat ttcatgacca tgagcatact
     5281 gaatatagcc tatggacttt ctcaggatac ttaccagctc gttggtagta ccagggcttg
     5341 ctgcatctaa atcatctaaa aagctataga aatccgttac atagttatca taatcataag
     5401 ggtcagaacg ccatttatag ttgtcctgat taataattct ttctattatt atgcgcaatg
     5461 cagctactat atcaacaaga gggtcaggat tgattgcagg catctgttgc aaggcatttt
     5521 ttgtaccggt aacatccgca ttgatagcat cgctcaacat aacattaaac ctatgctggt
     5581 caatagtatc aaatgcatca tacaatgccg gatagggatc taaaaggtca aataatcggt
     5641 attccggctc aggatcattg cgttgaaaca aaaatttgct actatccgaa catgataaca
     5701 acaccgttgc aacgcacatg attatgaaca atgatttaaa aggcttcatg gcgcacctct
     5761 tgtgatttat ttaaaaaatc atataggcga tagttactct ataataaggg ttgacagtac
     5821 tggcatgtcg gtatatttca acaaaaaacc gactacccag tactattttc cccaataaaa
     5881 aactcttttt ctttagcccc atttttgata ataccttatg gatcaaatac atgctctcct
     5941 cccttcctca ttcctgataa ctattatgac tactttaccg tccagctata ctcatattct
     6001 gtaaacagta taataccgac tgttaagtat tgtcaactat taatcaaaaa ttttacaatt
     6061 tttttaatat tttgtaataa tataatattt ttcacatatt atattgtaat tattatatta
     6121 tttaatatat ctataaatat gtttttagta tgtactcaat ttaatatata attaataata
     6181 ctgtaatata gttatagata actatattat gtaaaagtat atagctaatt attggtataa
     6241 atttatgtag ttttgtcgat attcaatctt gattaatgca catcattaaa ctttgtaatc
     6301 attattattc ctttaacaca ccaatcttct ttaactattt gttaaaactt tcaaaatctc
     6361 tatcccacgc ctggtaaatt ataattagaa ccattatcat tcctaactat aaactaacaa
     6421 aaaggctggt tttgtgtgga atctcctttt ttacttttaa ttctgatagt ataatacaat
     6481 ccctaatgga ataagaacag ttgaaataat tcattgtaca atcatagcac ctttacacag
     6541 ttctccttca ctttcatccc ctatggatgc cagcatttcg ggtatggcca accttcccga
     6601 aaatagaaat atacacccat atcataatgg actcgtttct gcatctctta tttcatagtt
     6661 attgaggctc tgaccccctt ttataataac tatcgcctat taaccgtata ctatgccttt
     6721 gacagacaaa cgatttatta catgagtgag tacttagtca taattgagtg aatgttcact
     6781 caattttgcc tattaatatt atataaatta atatcatatt tttattcaac atttataata
     6841 atattattga ctttattaat atttttttgt attgttaaaa gcaaagtgta tcaataaaca
     6901 tttttattta tgcttccaac atgggttttg tgaaggatgg ctttattgct atgccttaaa
     6961 tatttttaaa aggaggttcc ctatggatct ttttgataaa tgtttagcct tcactcgagt
     7021 tgaagaggtt aaatcatttg gattgtatcc ctattaccac ccaataacaa aaacagaagg
     7081 acctgttgtg tacatgaaca ataaacgtat gatcatggtc ggttcaaata actatctggg
     7141 actaacgcat gacccacgag taaaacaggc agctatcgat gccattgaaa aatatggcac
     7201 gggatgctca ggttcaagac tcatcaatgg ttcacttgat attcattgta agcttgagga
     7261 agcgctggcg caatttgttg gcaaagaagc tgcctgtatt ttttctaccg gttatcaaac
     7321 gaatcaggga gctattgtat cattaataaa caaaggggaa tatattattt cagatgaaga
     7381 aaatcatgca agcatcatac agggcacatt gatagcaaaa gccctatgtg gcgatagtat
     7441 tctggttaag tttcatcaca atgatatggt tgaccttgaa aataaaatct ctgctctgcc
     7501 gcatgatgca ggaaaactta tcgttgttga tggagtcttc agcatgaatg gacacattgc
     7561 tcccctcccg gatattgtta gaatagcccg taaatataat gcgcgcatca tgaccgacga
     7621 tgcacatgct cttggtgtta ttggccatga tggacgcggt acagcaagct attttggatt
     7681 aacagaccaa actgatatta ttatgggaac attttcaaaa agtttagctt catcaggagg
     7741 ttttttagca tcaagcgagc gggttataaa ctacatcaag cataacgcac ctgcaattat
     7801 atttaatgca agcatgcctg catcaaatgc tgctgcagca cttaaagcac ttgaaataat
     7861 tcaaaacgaa cctgaatcta tacaaaagct tcataataac gcaaaacgtt tgcggaatgg
     7921 acttgaacat cttggattta atgtaataaa aggggaaaca cctataatcc ctgttattat
     7981 tggtgatgat atcatgacgt ttacgttatg gaaaaagctc tttgattatg gcgtatatac
     8041 caatccaata atctttcctg cgacacctca ggggcagcag ttgatccgct gcagcgtcat
     8101 ggcaacacat accgatgaac atattgatta tgtattaggg atgtttgaaa aagctggtgc
     8161 tgaattaaaa ttattgcatg caaaggtata atcaaaattt tttatggctt tttgaaatca
     8221 atatagcatc gacatcaaaa acaaagcaga tgttaccagt ggaagtagga tcagcttttt
     8281 gatgcaatgc tgtaaggctc tgaccccttt tttataccca ctgtgcaatg gagtagcgtt
     8341 tgcgatacgc ttcctcatac tgcaaaaaac gccttacccg ttcgctaaag ctgtccccca
     8401 actgcagaaa gtactcatga tggtctatcg gtacaccaac atcagcaccc tcctgtacat
     8461 aggattgtat gctgctgtag cgatattttg taggactatc gcaccggcgc ttccgtaccg
     8521 gattgaacgc tatgtaccac agcaaccaca acaaataatg aaacgcatca tgggcaaact
     8581 gtacaatgat atccttatag cgctcattcc agaatggccc ggtcctgccg gttattttat
     8641 tatacatctc ggcaaaccgt gccttgatat actgtacaat gcgtgctatg ggcgctccac
     8701 cactggttgt gtggatgacg atatggatgt gattgtccat gatgcagtac gctatca
//
LOCUS       DAOOJH010000099         8482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7235_length_8482_cov_9.834698, whole genome shotgun sequence.
ACCESSION   DAOOJH010000099 DAOOJH010000000
VERSION     DAOOJH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7235_length_8482_cov_9.834698"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1002)
                     /locus_tag="PLJ75_10510"
                     /pseudo
     CDS             complement(100..1002)
                     /locus_tag="PLJ75_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424975.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(1004..1816)
                     /locus_tag="PLJ75_10515"
     CDS             complement(1004..1816)
                     /locus_tag="PLJ75_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05683.1"
                     /translation="MKNFASPEQLFYKLKSGYFAYIPKGCDAEGQTLQSRNSLIGQFT
                     EKWCKKLFDPIANNLKLHAINGVICEEIGLSRQSNADLAFCTTNDTVQKPQNIKIIFE
                     IKMSVVSNYKFLQPDTIEFVGDYKHHKGNPSLLRSDSMLKAIGKSINIRVSGVDSSNI
                     PIIILGNSPITENYAKKVDFLKTSGVIQGFWSLNPKPTNTDYIKETQKYGFKTIENID
                     QLKNDCNELIKSEMNFFSSMISRTRLGEIISIANRENSYAEKAEKFLLLIQQ"
     gene            2174..3202
                     /gene="recA"
                     /locus_tag="PLJ75_10520"
     CDS             2174..3202
                     /gene="recA"
                     /locus_tag="PLJ75_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPD05684.1"
                     /translation="MSEQKELNQKSQALESAILQIEKQFGKGSIMRLGDEHASLKVPS
                     ISTGSLELDIALGIGGIPRGRITEIYGPESSGKTTLTLHVIAEAQKLGGIAAFVDAEH
                     ALDPTYAKKLGVNTDELLVSQPDTGEEALEITESLVRSGAIDIVVIDSVAALVPRAEI
                     EGEMGDAHMGLQARLMSQALRKLTAVIAKSKTSVVFINQIRHKIGVMFGSPETTTGGN
                     ALKFYASVRLDIRRVDTIKVGEDAVGNRVRVKVVKNKVSPPFRQAEFDIMYDSGISRT
                     AGILDLGVKYNIIQKAGTWYSYKDERIGQGRENAKSYLDQHPDSAKEIEEKIKQQAGL
                     TEKEEAKV"
     gene            3212..4009
                     /gene="folE2"
                     /locus_tag="PLJ75_10525"
     CDS             3212..4009
                     /gene="folE2"
                     /locus_tag="PLJ75_10525"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPD05685.1"
                     /translation="MPRKSLKDIQSQPDDRNLPINKVGVKDITYPVVVMDRADGLQHT
                     VARINMYVDLPEHFRGTHMSRFVEVLNRYQGGISTKNVRNILDDLKGELNAKTAHVTI
                     EFPYFIVKAAPVSGEKSMMDYLCILQGDSSVDYYTLQVKVPLLSVCPCSKEISRYGAH
                     NQRNVVCVTVRAKEILRIEELIEIVEHCGSSEIYALLKREDEKYITERSYENPVFVED
                     IVRNVAIKLLAHPKVLWFQVETESMESIHNHSAYAQIEIDKQKKGQR"
     gene            complement(4006..4755)
                     /locus_tag="PLJ75_10530"
     CDS             complement(4006..4755)
                     /locus_tag="PLJ75_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HPD05686.1"
                     /translation="MTLKNKILIILSLCLLLCIGAYFIISRTFANFEKQLFEKCRIEA
                     LVGGRVMSEIIDMMIDTRLLTKQQVFDRNYVPIQGSNPQKFRTQYDAVFDKYIQKIED
                     EFLKDKDLEFAVLVDINGYLPTHNSKYAKPETSDPDHNLKYSRSKRIFNDRVGLKASR
                     FIGPGTLKQLYTRDTGEVMWDISSPVFVKGEHFGAFRLGVSLKHINELKNQMIIIVGM
                     TILVILSITMLMLFLILPRKLYDTDLDIPQY"
     gene            complement(5113..7113)
                     /locus_tag="PLJ75_10535"
     CDS             complement(5113..7113)
                     /locus_tag="PLJ75_10535"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HPD05687.1"
                     /translation="MQPLKKFIAIIILFISSSALIQCSTSTKITIHYSPAHYELSNSF
                     LAGASKADITPPPGKPLAGYSSNANFAKGFRTRLYARVIYLKSPLHNAIALVQCDLLS
                     GSSLVQQRVAELVASTTDVAYNGIVIAGNHTHSGPGNYFESNFYNRFASNASGFDEDY
                     FNFLCNQIAQAIIEAYNNRKPGMIAYGEKTLYGFTRNRSITAYTANANANSSNPLKAV
                     NPILSMIRIDTVENRSIHPIAALSIFSIHGTAVPDSNDLYSADVFAYIERELELEMQR
                     NGATTFIHAVVNGTHADNAPNIMKGKQGFQEARRIGIQIGHHAIELFNELSAKLSSHV
                     DSKSVIQFIDVYAHPTVDGATLCIPPKVGNTLLAGASDGGPTPIISSTPFFMEGSRRF
                     IFTCGCQKNKRIAAGPLQPLILPGDDFPHILTFQSIAINQLLLLPVPFEVTCESGKRI
                     ANAALTASPHTGIVTPIVISCANGYTGYCTTPEEYAKQRYEGGHTLYGPSTQPYLAKQ
                     YAQLSVTIAQNSMSDSSWTNCYVLKLASFYKKYPMPTTIRREALSKPVYVCNPGIDET
                     YVEFTWSDLPPDYMEFHQSLVSIEYSTDGIQFHPFIKNGITVDDTGYDIAVLFTGTIT
                     KEGNALYTTRWYNPELAENVYFRFAIQPRQKLPFVYSEPFSW"
     gene            7294..7776
                     /locus_tag="PLJ75_10540"
     CDS             7294..7776
                     /locus_tag="PLJ75_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05688.1"
                     /translation="MYFETTTCVSHNHLHCIARLADQYDMSIRSFIVYMIIYAAKKEK
                     APSKAFKSISYRDRKKDNPWKRLHLYLQYGEYEYLLDVKKVWKMSVAKVIEFCMENVL
                     DEFLAFLNEIDQEVKRGNTDNYLRYEINRSYMFDFCIDNGVHCCRFYWGLPMKYARAK
                     "
     gene            complement(7930..>8482)
                     /locus_tag="PLJ75_10545"
     CDS             complement(7930..>8482)
                     /locus_tag="PLJ75_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05689.1"
                     /translation="DPFKIKNKDNADIILITHEHFDHCSPDDIKKLQTNDTVIIAPKD
                     CIAKLSGNVKAITPGKSITVKGITIEAIPAYNTNKQFHPRSNQWAGYIITIDTMRIYM
                     AGDTDYIPEMKSLKNISIALLPVSGVYVMTADEAAQAALTIQPAVAIPMHYGSIVGTI
                     ADAKKFESLLKGKVQVVIKKQEN"
BASE COUNT         2492 a         1491 c         1823 g         2676 t
ORIGIN      
        1 acctcatggt gtaatttagt caagttgatt tttttgctta catgcatagt gaagccatag
       61 atatacatct ttattacacc gtgaatcatc taatgatgtt cagtttttaa attgtaatgt
      121 ggtttgttcc agaagcgggg caattcgttt tttagatagt tcaatatatt cagatgatat
      181 ttcataacca atgaatttcc tttccgcttt taatgcagca acacctgttg ttcctgaacc
      241 cataaaggga tccagaataa tatccccttt aaatgagtaa agctgaatta gcctgaaagg
      301 taattcttct ggaaatggag caggatgtcc tatacgtttt gcactttctg cattcattga
      361 ccatatggat ttagtccatt ccataaattg atcttttgtt atggtatctt cttttccttt
      421 tttatctcgt ttgtaatcac cttttgaaaa gattaaaata tattcatgga tgtcgcgtaa
      481 aataggattt gctgcacttt gccagattcc ccatgcagtt gatgggcttg cacttgctgc
      541 tttattccat ataatttcac ctcgcgtagc aaatcctata tctatcatta tctgcgaaat
      601 atagtctgaa agagggatat atggttttct gccaagattt gctatattga tacatgcgcg
      661 tcaaccattt accaaaaccc tgtacgtttc tttcaatact tttgtcaaaa acatcaaata
      721 ttctgataac gataggtcat tgtcatattc ttttgataca ttatacggtg gagaggttat
      781 catacaatga atagaattat cgggaatttc gttcatattt tcagatgaag catggataat
      841 ggtattttct aattccttgg ggaagaagtt ttcctcttta gaaacaggtc tattgtattc
      901 aagctctttg tagagtcgtg aattataaaa tttggaacta tcatgattta ttcttccatt
      961 ggttccaaat gagctggttt gagttccttt tgttcttttc atattattgt tgtattaaca
     1021 gtaaaaattt ttctgctttt tcagcatatg aattttcgcg gttggctatg ctaattattt
     1081 cgccaagcct ggttctggag atcattgatg agaagaagtt catttcactt tttattaatt
     1141 cattacagtc attttttaat tgatcaatgt tttctattgt tttaaaacca tatttttgtg
     1201 tttcttttat ataatcagta ttagttggtt ttggatttaa tgaccaaaat ccttgtataa
     1261 cgcctgatgt ttttaagaaa tcaacctttt tggcataatt ttctgtaatg gggctattac
     1321 ctaatattat aataggtatg tttgatgaat ctactcctga aactcgtata ttgatcgatt
     1381 taccaatagc ttttagcata gaatcagaac gaagcaatga aggattacct ttatgatgct
     1441 tatagtcacc aacaaattct atagtatcag gctgtaaaaa tttataattg gagacaacac
     1501 tcatttttat ttcaaaaatt attttaatat tttgtggctt ttgaactgta tcatttgtag
     1561 tacaaaaagc aaggtctgca ttagattgtc tggatagtcc tatttcttca caaataacac
     1621 cattaatagc atgtagtttt agattgtttg caattggatc aaaaagtttt ttacaccact
     1681 tttcagtaaa ttgcccaata agagaatttc tgctttgtaa tgtttggcct tccgcatcac
     1741 aaccttttgg aatgtatgcg aaataaccac tttttagttt ataaaaaagt tgttctggcg
     1801 aagcaaaatt tttcaatgct tctttaaaaa attgtatttc tatctggtta ttccagagtg
     1861 ccatttgttt tccctatact atacattctt gcaacattgt acttctaaca atggattgga
     1921 tgcagcaatg cattttgtat tctttcttat ctattgatgg tgaatgaata ctgtcaatct
     1981 ttttatggga aaattataat catggggctg gtatatctgt tgacttattt ttgctattgc
     2041 ctataatatg acataataag acaatcatca gtaactatcg cacaaagatt ttttttacat
     2101 ttttaaaaaa atttttaatt ttttgtagtc caggcgttct atagtataga atagtatagt
     2161 atgaggtata accatgagcg aacagaaaga acttaatcag aagtcgcagg cgctggagtc
     2221 ggcaatttta cagatagaaa agcagtttgg gaagggctcc atcatgcggc ttggtgatga
     2281 acacgcgtcc ctgaaggtgc cgtccatatc aacggggtcg cttgaacttg atattgcgct
     2341 ggggattggt ggcataccac gcgggcgtat caccgagatt tatggtcccg agtcatccgg
     2401 caaaacaacg ctaacattgc atgtaattgc tgaggctcag aaattgggag ggattgcggc
     2461 gtttgttgat gctgaacatg cgcttgatcc tacctatgca aaaaagctgg gtgtcaacac
     2521 cgatgagctt ctggtgtcgc agcctgatac cggagaagaa gcgctggaga taactgaatc
     2581 actggtgcgc tcaggggcta tagatattgt tgtcattgat tcggttgctg cactggtgcc
     2641 acgagctgag attgaaggcg agatgggcga tgcgcatatg ggattgcagg cgcgactcat
     2701 gagccaggcg ttgcggaagc ttaccgctgt tattgcaaaa tcaaaaacaa gcgttgtctt
     2761 catcaatcag atacgccata aaattggagt tatgtttggt tcgcccgaaa ccactacagg
     2821 cggcaatgcg cttaaatttt atgcttcggt acgccttgat atacgcaggg ttgatactat
     2881 taaggttggc gaggatgcgg ttggtaaccg ggtgcgcgtc aaggttgtga agaataaggt
     2941 gtcgccaccg ttccgtcagg ccgagtttga tatcatgtat gacagcggta tttcacgtac
     3001 ggcgggtata cttgatttag gcgttaagta taacattatc cagaaggctg gcacatggta
     3061 ttcgtataag gatgagcgca tagggcaggg acgtgaaaat gcaaaaagtt atttagatca
     3121 gcatcctgat agtgcaaaag agatagaaga aaagattaaa cagcaggcag ggctgaccga
     3181 aaaagaagaa gcgaaagtat gaagtaatgg tatgccgcga aaaagtctta aagatataca
     3241 gtcacaaccg gatgaccgaa acctgcccat caataaggtt ggagttaagg atatcaccta
     3301 tccggtagtt gtgatggaca gagccgatgg attgcagcat acggttgctc gcattaacat
     3361 gtatgttgat ctacccgagc atttccgtgg aacgcacatg tcacgttttg ttgaggtact
     3421 gaaccgctat cagggcggta taagcaccaa aaatgtgcgg aacatccttg atgatttaaa
     3481 aggcgagctt aatgcaaaaa ctgcgcatgt aactattgaa ttcccctatt ttatagtaaa
     3541 ggctgctcct gtcagtggtg aaaaatcaat gatggattat ctttgtattt tgcagggtga
     3601 ttcatcggtt gactattata cgctgcaggt gaaagtgcca ttattatcag tttgtccatg
     3661 ttctaaagag ataagccgct atggggcgca caatcaacgc aacgttgtct gtgtaactgt
     3721 cagggcaaaa gaaattttac ggatagaaga acttatagag attgttgaac actgtggctc
     3781 cagtgaaata tatgcattgc ttaagcgcga ggatgaaaag tacatcaccg agcgttcata
     3841 cgaaaatccc gtatttgttg aggatattgt caggaatgta gctattaaac tgttagctca
     3901 tccaaaggtt ttgtggtttc aggtagaaac agaaagcatg gaatcaatac ataaccactc
     3961 ggcatatgct cagattgaga ttgacaaaca aaaaaaaggg cagcgttaat attgcggtat
     4021 gtcaaggtct gtatcgtaca gttttcgcgg cagtattaaa aaaagcataa gcatggtgat
     4081 actgagtatt accagtatgg tcatgccaac aataatgatc atctgatttt tcagttcatt
     4141 tatgtgtttt aatgatactc cgagcctgaa tgctccaaaa tgctctccct tgacaaacac
     4201 aggggatgat atatcccaca taacttcacc cgtatcacga gtatagagct gttttaatgt
     4261 accaggacca atgaaacggg atgctttaag tcctacccta tcgttaaaga tgcgcttaga
     4321 acggctgtat ttaaggttgt gatcagggtc ggaggtttca ggttttgcat atttgctgtt
     4381 gtgtgtggga agatatccat tgatatcaac gagtacagca aattcaaggt ctttatcttt
     4441 taaaaattca tcttcaattt tttgaatata tttgtcaaag acagcatcat attgagttcg
     4501 gaatttctga ggattgcttc cttgaatggg aacataattg cggtcaaata cctgctgttt
     4561 ggtaagaaga cgggtgtcaa tcatcatatc aatgatttca ctcataacac gcccgccaac
     4621 cagtgcttca atgcggcatt tttcaaagag ctgcttttca aaattggcaa aggtacgtga
     4681 gataataaaa tatgctccaa tgcatagtaa aaggcacagg cttaaaataa taagaatctt
     4741 attttttagt gtcatagatg ttcccctgta ataaaaggtt acggcattac attaattcat
     4801 agagattgta caggtacatg aattgcaatg tcaattattt tatactattt tataattatt
     4861 gtcattggta acagtaataa tatccagggc gatagttacc ggtgatagcc tcacacccgg
     4921 cctcctctca aaaaaaggat agggggagtt gcaaatgggt gcacaatgca agatgaaaaa
     4981 tgtgaaagat gaaaatagct ggcgaatgca gtgcctgtga taattgcatc aacaatgtca
     5041 ttttggtgca aagacgatag ttattctgag tgatagcgaa aaatcccctt ttgacagtta
     5101 tctaatagca agctaccagc taaatggctc tgagtataca aaggggagct tttgacgcgg
     5161 ctgaattgca aatcgaaaat acacattttc agcaagctca gggttatacc agcgtgttgt
     5221 atagagcgca ttaccctctt ttgttatggt gccggtaaac aacacggcga tgtcgtagcc
     5281 ggtgtcatca acggtgatgc cattttttat aaacggatga aactgtatgc catcggtgct
     5341 gtattcaatg cttaccagcg attggtgaaa ttccatatag tctggaggca gatcgctcca
     5401 ggtaaattca acataggttt catctatgcc ggggttgcac acatataccg gtttgctcaa
     5461 tgcttcccga cggatggtgg tgggcatggg atatttttta taaaatgagg caagctttaa
     5521 cacatagcaa ttagtccagg aactatcgga catactattt tgtgcgatag ttactgacaa
     5581 ttgggcatac tgttttgcta gatatggttg tgttgagggg ccatacagcg tatgacctcc
     5641 ttcgtaacgc tgtttggcgt attcctcggg tgtggtgcag taacctgtgt atccatttgc
     5701 acagctaatg acgattggag taacaattcc ggtatgaggc gatgcagtaa gtgcggcatt
     5761 agcaatgcgt tttcctgatt cacaggttac ctcaaaagga acaggaagca ataaaagctg
     5821 attgattgca attgactgaa acgtcagtat atgaggaaaa tcatcgccgg ggagtatcag
     5881 tggttgcaaa ggaccagcag caatgcgttt atttttttgg caaccgcagg taaaaataaa
     5941 acggcgcgat ccttccataa aaaaaggagt actgctgata atgggagtag gaccaccatc
     6001 ggaagctcct gccaggagtg tattgcctac cttgggaggg atgcatagtg tagcaccatc
     6061 aacggtagga tgtgcataca catcaataaa ttgtataacg cttttgctat caacatgaga
     6121 ggaaagttta gccgatagct cattaaaaag ttcaatagca tggtgcccta tttgaatacc
     6181 tatccgacgg gcttcctgaa agccctgttt gcccttcatt atgttaggag cattgtcggc
     6241 atgagtacca ttgaccactg catggatgaa ggttgtggca ccgttacgct gcatctccaa
     6301 ttccagttcc cgctcaatgt aggcaaagac atcagcactg tataggtcat tgctatcagg
     6361 aacggctgtc ccatgtatgg aaaagatgct caacgctgca atgggatgga ttgatctatt
     6421 ttcaactgta tcaattcgga tcatagacag tatgggattt accgctttca aaggattgct
     6481 ggagtttgca ttggcatttg cggtatatgc agtaatgctt cggttgcggg taaatccata
     6541 taatgttttt tctccatagg caatcatgcc tggcttacga ttgttatagg cttcaatgat
     6601 tgcttgtgca atctgattgc ataaaaaatt aaaataatct tcgtcaaaac cagaagcgtt
     6661 tgacgcaaat cggttgtaaa aattgctttc aaaataatta ccaggtccgg agtgcgtatg
     6721 attgcctgct attacaatgc cgttataggc aacatctgtt gttgaagcta caagctctgc
     6781 taccctttgt tgtaccaaac ttgagcctga cagcaaatcg cattgtacaa gtgcaatagc
     6841 attatgcaat ggtgacttca aatagattac ccgtgcgtag agacgcgtcc tgaaaccttt
     6901 agcaaagttt gcattagaag aatatcctgc caggggttta ccaggcggag gggttatatc
     6961 tgctttgctt gcaccggcaa ggaaggagtt tgataattca taatgtgccg gggaatagtg
     7021 tatggttatc tttgtgcttg tactgcattg aattaaagcg ctcgaagata tgaacagaat
     7081 gattattgca atgaactttt ttaatggttg cataattgcc tcttaaaaat gagcggttgc
     7141 tcattatgac taataaacta tagcgctaaa agaagtcaac tataatcaat agttgtcttg
     7201 atagaatatg gaatgtactt acaatttttt cttgcatttt tttgccaggc aaactatcgt
     7261 aaatgaaaaa gtatactatg gaaggaggat atcatgtatt ttgaaactac aacttgtgta
     7321 tcgcataatc atttacattg tattgcaagg ttggctgacc agtatgatat gtccatacgt
     7381 tcatttattg tgtatatgat aatctatgct gctaaaaaag aaaaagctcc atcaaaggca
     7441 tttaaaagca tttcgtatcg agatagaaaa aaggacaatc catggaaacg cctccatcta
     7501 tatctacagt atggcgaata tgaatattta cttgatgtaa agaaggtctg gaagatgagc
     7561 gtagctaagg ttattgagtt ttgcatggaa aatgtattgg atgagttttt agctttttta
     7621 aatgaaattg atcaagaagt aaaaagagga aataccgata actatctgag atatgaaata
     7681 aatcgtagct atatgtttga tttttgcatt gataatgggg tgcattgctg ccgattttac
     7741 tggggattgc cgatgaagta cgccagagca aaataaaaca tcaatacgct cacaaaaagc
     7801 acatgccgct aaccgttgct gtaacatcgg gaggctcgtc tcttcctatc gtagtttatt
     7861 attcctgtag cacaggggat ttggcgttta cactacaaaa aggcatacac gccaaaaatt
     7921 acgcacacct taattttctt gtttctttat caccacctgc actttgcctt taagaaggct
     7981 ttcaaatttt tttgcatcgg cgatagttcc aactatcgag ccatagtgca tgggaattgc
     8041 aactgccggc tgtattgtca atgcagcctg cgctgcttcg tcagcagtca taacgtagac
     8101 gcctgataca ggcagcagcg caatagaaat attcttgagc gatttcatct cagggatata
     8161 atcagtatca cctgccatat agatacgcat ggtatcgata gtaataatat atcctgccca
     8221 ctgattgctt ctagggtgaa attgtttatt ggtattgtat gctggaatag cttcaatggt
     8281 tatgcctttt actgttatgc ttttgccggg agtgatagct ttaacattgc ctgaaagttt
     8341 tgcgatgcaa tcttttggtg caatgatgac ggtatcattg gtttgcagtt ttttaatatc
     8401 atcgggtgag cagtgatcaa agtgttcatg agtaataagg ataatatcag cattatcctt
     8461 gttttttatt ttaaaaggat cg
//
LOCUS       DAOOJH010000100         8479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7239_length_8479_cov_10.571700, whole genome shotgun sequence.
ACCESSION   DAOOJH010000100 DAOOJH010000000
VERSION     DAOOJH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8479
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7239_length_8479_cov_10.571700"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLJ75_10550"
     CDS             <1..630
                     /locus_tag="PLJ75_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPD05690.1"
                     /translation="SLEVTKRTAKEMIAQGRTIYKRFNSIAKNVNIKIPVNPAMQPDE
                     QCHFDGINAVKVLQSEGIPVNCTLVFTPEQALLAAKAGAVYISPFAGRIDDYIRSSAH
                     IKFDKSDYFPEEGIEQNGVLLHDNGIVSGIDVVSQCVEIVTYYEYKSEIIAASLRNPR
                     QVREAALAGAHIATVPFGVIAQMLRHEKTFEGMKKFTDDIVPEYEKLIG"
     gene            744..1055
                     /gene="clpS"
                     /locus_tag="PLJ75_10555"
     CDS             744..1055
                     /gene="clpS"
                     /locus_tag="PLJ75_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HPD05691.1"
                     /translation="MAHRYEYDGNLQIKEDVQVKKPRMYKVILHNDHYTTMDFVVEVL
                     VTIFHKNNMEATELMLQVHRNGFGICGVYTYDIAQTKAGQVHTLARQREFPLKCTVEE
                     A"
     gene            1107..3335
                     /gene="clpA"
                     /locus_tag="PLJ75_10560"
     CDS             1107..3335
                     /gene="clpA"
                     /locus_tag="PLJ75_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HPD05692.1"
                     /translation="MEISTVLNQILMAAYNEAKNRKHEYLTPEHVLLASLYFPEGREV
                     IEGCGGNVEQLKRNLSKHLSMHIPVIADGEPSQSVGLQHVIERAIMHINSAQKEILDF
                     GDIVIAIYEEEESFASYFLAREGITRYDLLNYISHGGSFEEEEIEEFGDEVGGNTEKQ
                     SKVLEQFTIELTQKAQKGELDPLIGREDILERTMQVLSRRLKNNPVHVGEPGVGKTAI
                     TEGLAQRIVAGAVPQSLKNAKIYRLDMGGLIAGTRFRGDFEERLKRVIAELQKQEKVI
                     LFIDEIHTIVGAGAVSGGSMDASNILKPVLADGKIRCIGTTTYDEYRKYFEKDGALSR
                     RFQKIDIHEPSHEDTIKILKGIKSRYEEHHNVIYTDDALAQAVELSARYITERKLPDK
                     AIDVIDETGAFARMYARDEENPTITAHQIEQMIAKMARIPERTVSASETAKLQYLGDE
                     LSMHIFGQDMAVQKVIEAIKRSRAGFRETEKPIASFLFVGPTGVGKTELARQLAFIMG
                     IELIRFDMSEYQEKHTVARLIGAPPGYVGYEEGGQLTEAVRKHPYCVLLLDEIEKAHP
                     DVFNALLQIMDYATLTDNTGRKADFRNTIIIMTSNAGVREMNKKVLGFEVATYNLDAV
                     NKELERLFAPEFRNRLDAIVYFNGLNKDMMRRIVIKKLDEFSKQLQEKHVELVCTDAC
                     IDWLSDKAYSPVFGAREVARVIQEHIKSFFVDAVLFGALKNGGRAIADIVGDAISITI
                     EQ"
     gene            3343..4074
                     /gene="aat"
                     /locus_tag="PLJ75_10565"
     CDS             3343..4074
                     /gene="aat"
                     /locus_tag="PLJ75_10565"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPD05693.1"
                     /translation="MVHFLNNDIICFPPVEEANEDGLLAFGGNLTTEWLLEAYTHGIF
                     PWFNSDRSEILWWSPDPRFVLFPHELKIHRSLKQVMKKKIFTVSFDTAFAQVISECSK
                     PRRQAFGTWITTRMKHAYIKLHELGFAHSVEVWHNGQLAGGLYGVSLGNTFFGESMFT
                     LMDNASKIALVTLVTVLRRLEFALIDCQVYTDNLKHFGARFIPRDEFIRLLTMSPGYT
                     TLCGSWSHLVVDGYDYVQCISEVNA"
     gene            complement(4055..4960)
                     /locus_tag="PLJ75_10570"
     CDS             complement(4055..4960)
                     /locus_tag="PLJ75_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPD05694.1"
                     /translation="MDISFKDYYTILGVSPAADTGTIKSAFRQKAKETHPDSTKQNSY
                     AQFVLIREAYDILTNPSQRASYDIVWNKYHVKSEPEMTSLFDDFGPPDDRYAQEWEYF
                     VLHPEDYLSRFESTLALIKASLRSIAISVCIPVIVMISILLGVTIAMVTLFVFAIGIT
                     SYSSLIAGIVITLLMVKRFVEISQELFTRYSEIFARWMVKQLKGIPMAIGKWVLYGTF
                     SFIFILLVIYAAAVVVSICSLSSLVYFRIIVVAAGVMAVAVISLSLYFVFTVFTNALL
                     HYPKIRYTKIKAKNNHLLESKRLLH"
     gene            complement(4974..5360)
                     /locus_tag="PLJ75_10575"
     CDS             complement(4974..5360)
                     /locus_tag="PLJ75_10575"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05695.1"
                     /translation="MSFEIELLNLSLINKENVDYDMIIFYMNYPEKIDIDTSREIMLF
                     LTTLIKGGAKKIIVDMKKVTLIDSAGISTLILATKQIRKNNGDLVLSHVNDSIQNILD
                     IIKIEKFIKIFRTDSDAVQYFRYIQT"
     gene            complement(5387..5764)
                     /locus_tag="PLJ75_10580"
     CDS             complement(5387..5764)
                     /locus_tag="PLJ75_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05696.1"
                     /translation="MNCTIIELPGIKIDTINYNRAVCIIKLADVADMDKNDDLLALTM
                     CLINGGLRKIIFDFTSVEFIDSYGIGTIVEIAKYIRQHHNGDVVLINVSERILLIFKP
                     IQFQKFIKIFHSEDEALHYFQYV"
     gene            complement(5832..>8479)
                     /locus_tag="PLJ75_10585"
     CDS             complement(5832..>8479)
                     /locus_tag="PLJ75_10585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD05697.1"
                     /translation="IPLYIIHNQIQQQKEHLVNEGTIYSMNLAHSIAAIVLLNSGNVA
                     QTSIDALEFVETLKPPFTRELASIQVILAAPNKKYNGLLLVDIPNNKTLTKLPDIIVN
                     KLKSTSTYSYISSNNIRYFEFVAQSPVAMGNIHCIARLLYNEDAVLKPVYRNYTIFVI
                     STVIIIFLAVIFSLSFTRAFSRPIETMIQSIKQFDEGVTNIEIPIKNKTDEIGKLATT
                     IQHLFYMVNLEIRALQQNNRELIRLDKLKDDFLANLSYELKIPLNGIIHLTNNIIHDY
                     AEKNHPYTTNTLQLITNSAMRLSYLIDDISDFTRLKNNDIVLNKKIVDITGVINFVIS
                     LLQPAVASKDITIILSIQSNASPLYVDEQRLQQILLNIIGNSIKYTKQGTITIATQMH
                     DSNTLSIIITSTAGGIPPELVKNIDDFGYDSTVSNKQSGGSALGLAVTKKLIELHDGS
                     IYIESQPDGGSCVTVDFPYDENLISKELATASTNDFVISLPEDIIQYQPSLMLDTSQG
                     FVYIVDDDPVNVKILQEMLQKANFYIEFSHDPSLLYDSIQKNRIPDIVLLDTILPGTS
                     AFQVCETIRKSYSLHELPIIIMTSKHRTQEIITSFRVGANDYLSKPYNEEELIARIHN
                     LIMLKRSVKEHNDYVILKHEIRLAHEIHNSVVIQNIPQLHNITIAHAYIPTRDMGGDF
                     YDVIKIDDDKIGIMIADVTGHGIPAALVCAMLKMAFINNKEHARYPARMMTLLNKDLS
                     STIHDNYITSAYSLINTKNKTITVSIAGHPPPLLLHNDGTVFKNWTRAFPIGWIGDAK
                     YQETSIHYQHGDKFILYTDGVIELKKGKQIFGEDNVITCIANSFEKQPAILIDEMIKL
                     LHDWSSIPADEPFKDDVTLLIAELL"
BASE COUNT         2601 a         1427 c         1800 g         2651 t
ORIGIN      
        1 agccttgaag tgaccaaacg cactgcaaag gagatgatag cacaggggcg tactatctat
       61 aagcgtttca acagtattgc aaagaatgtc aatataaaga taccggttaa tcctgccatg
      121 caaccggatg agcaatgtca ttttgatggc atcaatgcag tgaaggtttt gcaatctgaa
      181 ggcatcccgg taaactgtac gctggtgttt accccggagc aggcgctttt agcggctaaa
      241 gcgggtgcag tctatatcag cccatttgca ggacgtattg atgattatat acgaagcagc
      301 gcacatatca aatttgataa aagcgattat tttcctgagg aagggattga gcagaatggt
      361 gtactgttac atgataatgg gatagtatca gggatagatg tagtatcgca gtgtgttgaa
      421 atagtaacat attatgaata caaatctgaa attattgcag catcgctgcg gaatccccgt
      481 caggtacgtg aagcagcgct tgcgggcgct catatagcaa ctgtaccatt tggtgtgata
      541 gctcaaatgt tacggcatga aaaaacattt gaaggcatga agaaatttac cgatgatatc
      601 gtccctgaat atgaaaaact aattggatag agaggttgcc ataaataaaa atttttttta
      661 tattgtaata aatagtatag tacaaactac aatagagtaa tggtggcaat aataatagaa
      721 catggtaaac caggaatata tccatggcac atcggtatga atatgacggc aatctccaga
      781 ttaaagagga tgtacaggta aagaaaccac gcatgtataa ggttattctt cataatgacc
      841 attatacaac catggatttt gttgtagagg ttctggttac aatatttcat aaaaataata
      901 tggaagcaac ggaacttatg ttgcaggttc acagaaatgg ttttggtatt tgtggtgtgt
      961 atacctatga tatagcacag accaaggcag gacaggtaca tacactggca cgtcagcgtg
     1021 aatttccgct gaagtgtact gttgaagagg catgacaggt atactggtat tttaattgta
     1081 ataaattgta ataaagggtt ttgtcaatgg agataagcac cgtattaaac cagatattga
     1141 tggctgccta taatgaggca aagaatcgta agcatgaata tcttacgcct gaacatgttt
     1201 tattggcctc gctgtatttt cctgaaggca gggaggttat agaaggatgc ggtggcaatg
     1261 ttgagcagct taagcgaaat ttaagcaagc atctttcaat gcatattcct gtaattgcag
     1321 atggagaacc ttcacaatct gttggattgc agcatgttat tgaacgtgcc attatgcaca
     1381 tcaattccgc acaaaaagag attctggatt ttggtgatat agtcattgcc atttatgaag
     1441 aagaagaatc atttgcttca tactttttag ctcgtgaagg tatcactcgc tatgacctgt
     1501 tgaattatat atcacatggc ggaagttttg aagaagaaga gatagaagag tttggtgatg
     1561 aggttggggg taataccgaa aaacaaagca aagtgctgga gcaatttact attgaattaa
     1621 cgcaaaaagc gcaaaaggga gagctcgatc cccttattgg cagggaggat attttagaac
     1681 gtaccatgca ggtgctgtcg cgaagattaa aaaacaaccc tgtccatgtc ggtgaacctg
     1741 gtgttggtaa aactgcaatt accgagggat tggctcagcg cattgtggca ggtgctgtgc
     1801 cgcaatcact gaaaaatgca aagatctacc ggctggatat gggtgggctt attgccggta
     1861 cacgattccg aggtgatttt gaagagcgcc ttaagcgtgt gattgctgaa ttgcaaaagc
     1921 aggaaaaggt gatactgttt attgatgaga tacatacaat cgttggtgcg ggtgcagtga
     1981 gcggcggttc aatggatgct tccaatattt taaaaccggt gttagccgat ggtaaaatcc
     2041 gttgcatagg caccacaacc tatgacgaat atagaaaata ttttgaaaag gatggcgcac
     2101 tttcacggcg ctttcagaag attgatatcc atgaaccatc gcatgaggat acaatcaaga
     2161 tcctcaaggg tataaaatcc cgatacgagg agcatcataa tgtaatctat actgatgatg
     2221 cactggcaca ggcagttgag ctatcagcgc gatatattac tgagcgaaag cttcctgaca
     2281 aagcgataga tgttatagat gagacaggag cttttgcgcg gatgtatgcc cgtgatgaag
     2341 agaaccccac catcacagcc catcagattg aacagatgat agctaaaatg gcgcgtattc
     2401 ctgagcgcac agtttctgca agtgagactg caaagttgca gtatttaggg gatgagcttt
     2461 caatgcacat ctttggacag gatatggcag tccaaaaggt tatagaggcc atcaagcgtt
     2521 cgcgcgcagg cttccgggaa acagaaaaac ccattgcatc gtttttattt gttggtccaa
     2581 caggcgttgg caaaaccgag ctggcgcgac agcttgcatt tatcatgggt attgaactta
     2641 ttcggtttga tatgagcgag tatcaggaaa agcatactgt tgcccggctc attggagcgc
     2701 cacccggcta tgtaggatat gaagaaggag gacagcttac tgaagcggta cgcaaacatc
     2761 catactgtgt gttgctgctt gacgagattg aaaaagcaca tcctgatgtt tttaatgccc
     2821 ttttgcagat tatggactat gcaaccctta cggataatac aggacgcaaa gctgatttcc
     2881 gcaatacaat tatcattatg acatccaatg caggtgttcg tgaaatgaat aaaaaggtgc
     2941 ttgggtttga agtggctacc tataatcttg atgccgttaa caaagagctg gaacgccttt
     3001 ttgctcctga gttcagaaac aggctggatg caatagtgta ttttaatggg ctcaacaaag
     3061 atatgatgcg caggattgtg attaaaaagc ttgatgaatt ttctaaacag ttgcaggaaa
     3121 aacatgtaga gcttgtctgt accgatgcat gtatcgattg gctttcagat aaggcatatt
     3181 ccccggtgtt tggagcacgt gaggttgccc gtgttataca ggaacacatc aagtcatttt
     3241 ttgtggatgc agttcttttt ggagcgctaa aaaatggagg cagggcaata gctgatattg
     3301 tcggtgatgc aatatcgata actatcgaac aatagtacat gtatggtgca ttttttgaat
     3361 aacgatatta tatgttttcc tccggttgaa gaggcaaatg aagatggatt gctggcattt
     3421 ggcggcaatc taacgacaga atggttgctt gaagcctaca cgcatggcat attcccctgg
     3481 tttaatagcg acagatcaga aattttgtgg tggagccctg atccgcgttt tgtgttattt
     3541 cctcatgaat tgaagattca caggtcactg aagcaggtga tgaagaaaaa aatatttacg
     3601 gtgagctttg atacagcttt tgcacaggtt atatcggaat gttcaaaacc gcggaggcag
     3661 gcttttggca catggataac tacaaggatg aaacatgcat atataaaact gcatgaactg
     3721 ggatttgcac attcggtaga ggtgtggcac aacggtcaac ttgcaggagg gctttatgga
     3781 gtatcgctgg ggaatacttt ttttggcgag tcaatgttta cccttatgga caatgcctca
     3841 aagattgcac tggtaacgct ggttaccgtt ctgcgccgat tggaatttgc gcttattgat
     3901 tgccaggtat atactgataa tctaaagcat tttggtgcac gattcatacc aagggatgaa
     3961 tttatacggc ttcttaccat gtcgcctggt tacactacat tgtgtggtag ctggagtcat
     4021 cttgttgttg atggctatga ctatgtgcag tgtatcagtg aagtaaacgc ttagattcca
     4081 gcaggtgatt gttttttgct tttatctttg tatagcgtat ttttggatag tgtaacaatg
     4141 cattggtaaa aacggtaaaa acaaaatata atgaaagcga gataaccgct acagccataa
     4201 cacctgctgc aacgacaatt atacgaaaat acaccaatga agaaagtgaa catatggata
     4261 ctacaacggc agccgcatag ataaccaata atataaaaat aaagctaaag gtaccataca
     4321 aaacccattt ccctattgcc atgggtatcc cctttaattg tttgaccatc caccgtgcaa
     4381 atatttctga gtacctggta aaaagttcct gtgaaatttc tacaaaccgt ttaaccatta
     4441 atagagttat gacaatacca gcaataagcg aagagtacga tgttattccg attgcaaaaa
     4501 caaaaagagt aaccatggcg atagttactc ccaaaagaat acttatcatc actataacgg
     4561 ggatgcagac gctgattgca atagatctaa gtgatgcctt tattaaagca agtgtgctct
     4621 caaagcggct tagatagtct tcaggatgta atacaaagta ttcccactcc tgtgcatagc
     4681 gatcatcagg tggtccaaaa tcatcaaata aagatgtcat ctctggttca gattttacat
     4741 gatatttgtt ccagacaatg tcatagcttg ctcgctgcga cgggttggta aggatatcat
     4801 acgcttcacg gataagtaca aactgtgcat agctgttttg ttttgtgctg tcagggtgag
     4861 tttcttttgc tttttgtctg aatgctgatt taatggtgcc agtatcggca gcaggggaaa
     4921 caccaagtat tgtataataa tctttaaatg atatgtccat aagcaacaat cagctatgtt
     4981 tgaatgtatc ggaagtattg cacagcatca ctatcagtgc gaaaaatttt aataaatttt
     5041 tcaattttta taatatccag aatgttctgg attgaatcat taacatgtga taaaactaaa
     5101 tcgccgttgt tttttctgat ttgttttgta gctaaaataa gtgtacttat acctgcgctg
     5161 tctataagag taactttttt catgtcaaca atgatctttt ttgccccacc cttgataagg
     5221 gtagttaaaa atagcattat ttcgcgggag gtgtcaatat caattttttc agggtaattc
     5281 atgtagaaaa taatcatatc atagtcaaca ttttccttgt taattagtga aagatttaat
     5341 aattctattt caaaactcat agtaaaccac ctgtatttta tagtgattaa acgtattgaa
     5401 aataatgcaa ggcttcgtcc tctgaatgaa atattttgat aaacttctgg aattgaattg
     5461 gtttaaaaat taataaaata cgttcggaca cattaattaa tactacatcg ccattatggt
     5521 gctggcgaat atattttgca atttctacta ttgtaccaat tccatagcta tcaataaatt
     5581 caactgaagt aaaatcaaaa attattttac gtaagcctcc atttatcaaa cacatcgtga
     5641 gtgcaagtaa atcatcattt ttatccatat ccgcgacatc ggctaatttt attatgcaga
     5701 ctgctctgtt ataattaatg gtatcaattt tgataccggg taattctatt attgtacaat
     5761 tcatacttct tactatttta ctattatgca atattcataa atcaataact atttttcatc
     5821 aaaaaatgat ttcataacag ttcagcaatc agtagcgtta catcatcttt aaaaggttca
     5881 tcagcaggga ttgagctcca atcatgtaat agtttaatca tttcatcaat tagtatagct
     5941 ggttgctttt caaaactgtt tgctatacat gtaatgacat tatcctcgcc aaatatctgt
     6001 ttgccctttt tcaattctat aacaccatca gtatataata taaacttatc gccatgctgg
     6061 tagtgtatgc ttgtttcttg atatttagcg tcccctatcc agccaatagg gaatgccctt
     6121 gtccaatttt taaatactgt accatcgttg tgcagtaata agggaggggg gtgccctgca
     6181 attgatactg taattgtttt attttttgtg ttgatcagac tataggctga tgtaatataa
     6241 ttatcatgga ttgtgctgga taaatcttta ttgagtaatg tcatcatcct ggcaggatac
     6301 cgtgcatgtt ctttattgtt gataaatgcc attttaagca ttgcgcatac cagtgcagcg
     6361 gggataccat gtccggttac atcagcgatc ataataccaa ttttatcatc atcaattttt
     6421 attacatcat aaaaatcacc acccatgtcc cgcgttggga tgtaggcgtg agcaatagta
     6481 atgttgtgca attgtggtat attctgtata acgacagagt tatgaatttc atgggctaac
     6541 cttatttcat gctttaatat cacataatca ttatgttctt ttactgatcg tttcaacata
     6601 ataagattat gtattctggc tattaattct tcttcattat agggtttact aaggtaatcg
     6661 ttagcgccta ctctaaagct ggtaatgatt tcctgtgttc ggtgttttga agtcattatt
     6721 ataataggca attcatgaag tgaatagctt tttcgtattg tttcacatac ctgaaaggca
     6781 gaagtgccgg gcaatatggt atcaagaagt acaatatcgg gtattctgtt tttctggata
     6841 ctatcatata gcaatgaagg atcatgggaa aattctatgt aaaaattggc cttctggagc
     6901 atctcctgca aaatttttac attaacagga tcgtcgtcta ctatataaac aaatccttgt
     6961 gaagtatcca gcattaacga tggttgatat tggatgatat cttcaggtaa gctaatcaca
     7021 aaatcatttg tagatgctgt tgctaattct ttactaatca gattttcatc ataagggaaa
     7081 tctactgtaa cacacgaacc gccatcgggt tgagactcaa tgtaaatgct gccatcatga
     7141 agctctatca gcttttttgt tacagcaagt ccaagagctg atccgccaga ttgtttgttg
     7201 gatacagtgc tgtcatatcc aaaatcatca atatttttta ccagttcagg agggatgcct
     7261 cctgcagtac tggtaattat gattgaaagg gtattactat catgcatttg agtggcaatg
     7321 gtaatagttc cctgtttggt gtatttaatc gaattgccaa ttatatttaa aagaatctgt
     7381 tgtaaacgct gttcatcaac atacaggggg gaggcattac tctggataga caggataatc
     7441 gtaatatctt ttgatgctac tgctggttgt aatagagaga taacaaagtt tataacgccg
     7501 gttatatcta caattttttt attgagcacg atgtcattat tttttaatcg ggtaaaatca
     7561 gaaatatcat caatcagata ggataatcgc atggcgctgt tggtgataag ctgaagggta
     7621 ttagttgtat atgggtgatt tttttcagca taatcatgga taatattatt ggttaagtgt
     7681 attatgccat ttaaagggat cttcagttca tacgataaat ttgccagaaa atcgtctttc
     7741 aacttatcaa gtcgtatcag ttccctgtta ttttgttgaa gtgcacggat ctctaaattt
     7801 accatataaa aaagatgttg tatggttgtg gctaattttc ctatttcatc agttttgttt
     7861 tttataggga tttcaatatt ggttacacct tcatcaaatt gttttatgga ctggatcatt
     7921 gtttcaattg gccgggaaaa tgcacgggtg aatgataatg aaaaaataac agctaaaaaa
     7981 attataatga ctgtactgat aacaaagata gtataattgc gatacacagg ttttaaaaca
     8041 gcatcttcat tatagagcaa tcgtgctatg caatggatat tacccatggc tactggagac
     8101 tgagctacga attcaaaata ccgtatgtta tttgatgata tatatgaata ggtgctggtt
     8161 gattttaatt tattgacaat tatatcgggt aattttgtta aagttttgtt gtttggaata
     8221 tcaacaagta acagcccgtt gtatttttta tttggtgcag ctaaaataac ctgtatggat
     8281 gcaagttctc tggtgaatgg gggttttaat gtttctacaa attctaaagc atctatgctg
     8341 gtttgagcaa cgtttccact gttgagcaat acaatagcag ctatagaatg tgcaaggttc
     8401 atggagtata tggtaccttc attaacaaga tgttcttttt gttgctgaat ctgattatga
     8461 ataatgtaca atggtattg
//
LOCUS       DAOOJH010000101         8383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7324_length_8383_cov_10.060879, whole genome shotgun sequence.
ACCESSION   DAOOJH010000101 DAOOJH010000000
VERSION     DAOOJH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8383
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7324_length_8383_cov_10.060879"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1153)
                     /gene="metK"
                     /locus_tag="PLJ75_10590"
     CDS             complement(2..1153)
                     /gene="metK"
                     /locus_tag="PLJ75_10590"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPD05698.1"
                     /translation="MGKDYLFTSESVSEGHPDKVADQISDAILDEHIKGDPYSRVACE
                     TLVTTNRVIISGEITSSVTVDYEAVARKVIASIGYTSPEIGFDAKACNVEIYIHPQSP
                     DISQGVTEGQGLFKEQGAGDQGMMFGYAVAETDELMPMPILYAHRLVKRLADIRKKGE
                     VNFLRPDGKSQVTVEYQNDKPKRISTIVVSTQHDAAVTLPTLEEMVRELVIKQVIPSN
                     LWDKNTKIYVNPTGRFEIGGPHGDTGLTGRKIIVDSYGGMGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKAIVEAGLASRCELQVAYAIGVADPVSVMVETFGTSTIPEETIEKRIQ
                     HVFNLKPKSIIQTLDLLKPKYTITAAYGHFGRNEPGFTWEQTDKAQELR"
     gene            complement(1256..1447)
                     /locus_tag="PLJ75_10595"
     CDS             complement(1256..1447)
                     /locus_tag="PLJ75_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HPD05699.1"
                     /translation="MAKEVYVDQNECTGCELCVDTLPEVFEMTGDGVSTVKNSKGASE
                     DKIQEVIDSCPAECIHWKD"
     gene            complement(1526..1867)
                     /gene="secG"
                     /locus_tag="PLJ75_10600"
     CDS             complement(1526..1867)
                     /gene="secG"
                     /locus_tag="PLJ75_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPD05700.1"
                     /translation="MSILISIGTVLFVVLSILLIIIILLQSDKSAGMGILGGSSNTAF
                     GSSTADIITKITTVMVALFMLGSLGLSIFESYRSSSLEKDLLQQEKTGGVIEQKTEVP
                     QQGQPVQPQAK"
     gene            complement(1921..2688)
                     /gene="tpiA"
                     /locus_tag="PLJ75_10605"
     CDS             complement(1921..2688)
                     /gene="tpiA"
                     /locus_tag="PLJ75_10605"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPD05701.1"
                     /translation="MIKQQVRKQIFAGNWKMYTTKREAVELAIGIASGMNFMDREVVL
                     FPPSVYATCVIGACEGTPVKVGLQNMFYEKEGAYTGELSPQMLKDVGAGYVLVGHSER
                     RHIFGETDDLIHKKVISAIAFDIIPVLCVGELLEERESGKSESTVENQLIKAFAEIDE
                     QGARKAVIAYEPVWAIGTGKVATPEIAESMHIFIRNTLKKLYNDSVAMTIPVLYGGSV
                     KPDNIAGLYAMPNIDGVLVGGASLAVQSFLDIINVTL"
     gene            complement(3083..>3949)
                     /gene="gap"
                     /locus_tag="PLJ75_10610"
     CDS             complement(3083..>3949)
                     /gene="gap"
                     /locus_tag="PLJ75_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPD05702.1"
                     /translation="FKYDSVFGVFNGTVSHKENAITINGKEIKILAEKNPATLPWKAE
                     SIDVVVESTGKFRDRDKVMPHLDAGAKKVIITAPAKGEDITVVLGVNEDKYDTAKHNI
                     ISNASCTTNCLAPVAKVVNDVFGIEKGLMTTVHSYTNDQRLLDLPHSDFRRARAAALS
                     MIPTTTGAAKAVALVLPELKGKLDGLSIRVPTPDVSVVDFVCTVKTQTTAEDVNKALR
                     EAANGKLKGILQVCDEELVSIDFKGNNHSSIVDAPSTAVIGGNMVKILSWYDNEWGYS
                     CRVVDLIQYCMK"
     assembly_gap    3950..4049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4050..4789)
                     /locus_tag="PLJ75_10615"
     CDS             complement(<4050..4789)
                     /locus_tag="PLJ75_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPD05703.1"
                     /translation="MKTGYFATTIGEMYYRYNEIQSENILVCIHGAGGDSRQFIPLMN
                     ALQTIAVYAVDLPGHGKSAINECSLDIYVQAMQQFIGSLNGNVFVLGHSMGGGIIFEL
                     VQRNALIAGALFLATAGKLPVNPVVFDLLDKDFDGLCKLAVDLSYGAIDDTMRKTAIE
                     CMKQAGSNVLKNDFTLCDGYNYDETAAQFPFTSCFIANRKDTMVPLDYIYATFKKMPD
                     AMLQVLPYKGHMSHIEQVHQVAACINHF"
     gene            5014..5253
                     /locus_tag="PLJ75_10620"
     CDS             5014..5253
                     /locus_tag="PLJ75_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05704.1"
                     /translation="MKLTIFLKNTSIVSIHLYLRTGISIPCCQHAVTLLKQITLPPCD
                     DDATHCTFVAYTDTSFTTIGCLLYPHCYRFDYRSL"
     gene            complement(5583..6101)
                     /locus_tag="PLJ75_10625"
     CDS             complement(5583..6101)
                     /locus_tag="PLJ75_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011410949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05705.1"
                     /translation="MGLAVKIYEAFKDDETKARVLADVIESLEQIIKVNEVATRSDIK
                     NTELALIREIEQIRKDMKETELKLTKEIEDVRADLSKEIEKVRADLSKEIEKVRADLS
                     KEIEKVRADLSIDIEKSRAESSISLEKLRSDLIEKIEEKHSSSIRWLIALFITQSIGI
                     AGLIVTLFQILR"
     gene            6370..6843
                     /locus_tag="PLJ75_10630"
     CDS             6370..6843
                     /locus_tag="PLJ75_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPD05706.1"
                     /translation="MNITSNSFASGGIIPVKYAMKGIPGGQNISPHIAIHDVPKEARS
                     LAVAFVDRHPMARNWVHWLVLNIPAHTKEIQEGASLKAMPHDSIEMINTFGFAGYGGP
                     QPPKGSGVHSYELCCYALAELFKIPKKELTENELLKLIKPLLITQCTIHGNFENK"
     gene            6961..7659
                     /locus_tag="PLJ75_10635"
     CDS             6961..7659
                     /locus_tag="PLJ75_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPD05707.1"
                     /translation="MGIPVYQYDNIIEVQKEGVCDSSEINAIAAIDAQLKNDEKIRSL
                     LISETCIKNRTAALAGDIVRDMNHSNHIDIMMVLTGSFMFTADLARALYAQGGINTSI
                     HCIKTSVYDETIKSSGEHYRAVTLELSPKNIEARDILLVEDIADQGFTLSWLKNYLLY
                     ERNARSLKICSLLYKRLDNPTREVQTIRKQLGIDYIGFTVPDVWVAGYGIDAAYQYRH
                     LPFIVWINEAYFQS"
     gene            7878..>8383
                     /locus_tag="PLJ75_10640"
     CDS             7878..>8383
                     /locus_tag="PLJ75_10640"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HPD05708.1"
                     /translation="MKKLFTITVLVFIVVSSLFALTGEEAIIRFQQRMRSINTLSGNI
                     SWTTSNGFTYTGTFKYKAPDKLYLNLTAPGGKAIVTNGKKLWVYDKSSNICGVQDVGD
                     GYSGGIASIVSGYNALLASQGTGGYTIKLRNFEKAYPEIVIVADSNFLLKKLILKDKQ
                     GDSTTYTIS"
BASE COUNT         2502 a         1786 c         1427 g         2568 t
ORIGIN      
        1 atcatcgcaa ttcctgggct ttatcagttt gttcccatgt aaaaccaggt tcattgcgcc
       61 caaaatgtcc gtacgctgca gttattgtat actttggttt taacagatca agcgtttgta
      121 tgattgattt tggttttaaa ttgaagacat gttgtatgcg tttttcaatt gtttcttccg
      181 gtatggtgct ggtgccaaag gtctccacca tgaccgaaac aggatcagca acccctatgg
      241 catatgctac ctgaagttcg caccgcgatg caagccctgc ttcaacgata gccttggcaa
      301 tgtaacgccc catataagct gcagagcggt caaccttaga tggatccttg cctgaaaatg
      361 ccccgccacc atgtctgccc attccaccgt aactatcgac aataatcttc ctgccggtta
      421 atccggtatc accatgggga ccgccaatct caaaacggcc cgttggatta acataaattt
      481 ttgtgttttt atcccagagg ttagaaggta taacctgttt tattacaagc tcacgcacca
      541 tctcttccag cgtgggtagc gtaacagctg catcatgctg cgtggaaaca acaatagtgc
      601 ttatacgttt tggtttgtca ttttgatatt caacggtcac ctgtgattta ccgtcagggc
      661 gtaaaaagtt tacctcacct ttttttctga tatctgcaag gcgttttacc agccggtgtg
      721 catataaaat aggcatgggc ataagctcat cggtttcagc tactgcatag ccaaacatca
      781 tcccctggtc gccagcaccc tgctctttga aaagcccctg tccttcggta acaccctgtg
      841 aaatatcagg tgattgagga tgtatatata tttctacatt gcatgctttt gcatcaaagc
      901 caatctccgg tgaagtatat ccaatactgg caattacctt acgggcaaca gcttcataat
      961 caacagttac cgatgatgta atctcaccgg atattataac ccggtttgtg gttacaagcg
     1021 tttcgcatgc aacacgagaa tacggatcac cttttatatg ttcatctaaa attgcatcgg
     1081 atatctggtc tgcaacctta tcaggatgtc cttctgaaac tgattcagaa gtaaataaat
     1141 agtcttttcc catttttcac gcctccttta tacattttta tattatgtac ataacaaaag
     1201 gcatattgag tcaatatgcc ttacatcagc taaacttctt cattgactat ggatgttagt
     1261 ccttccagtg aatgcattct gcaggacagc tatcaataac ttcctgaatt ttatcctcag
     1321 atgcaccttt tgagttttta acggtgctca caccatcacc agtcatttca aaaacctccg
     1381 gaagcgtatc aacgcaaagc tcacatcccg tacattcgtt ttgatccaca tacacttctt
     1441 ttgccataac ctcctccata cgcaaataat attgtttact tcatagtact atatgttact
     1501 ataattgcat gcttaactca ttaatttatt ttgcctgcgg ctgtacaggc tgtccttgtt
     1561 gaggaacttc ggttttttgt tctatcacac cacctgtctt ttcctgctgt aacaaatctt
     1621 tttcaagcga acttgatcta tacgactcaa atattgatag tcccagtgaa ccaagcataa
     1681 agagtgcaac catcactgtg gtaattttgg ttataatgtc agcagtggac gagccaaatg
     1741 cagtattgct ggaacccccc aatataccca tcccagcact tttatcagat tgcaacaata
     1801 taataatgat aagcagtatt gacagtacaa caaaaagcac cgtcccaatt gatattaata
     1861 tactcataat acacctttaa tattcagatt tttctacaat acaatatagt tttggtctat
     1921 ttataatgta acattaatta tgtcaagaaa agattgcact gcaaggcttg caccacctac
     1981 cagtacacca tcaatattgg gcatcgcata taagccagca atattatcag gcttcaccga
     2041 ccctccatag agcaccggga tagtcatagc aacactatca ttatacagct tttttaaggt
     2101 atttctgata aaaatatgca tcgattcggc aatttctggc gttgctacct tcccggtgcc
     2161 aatcgcccaa acaggctcgt aagcaatcac cgctttacgt gcgccctgct catcaatttc
     2221 agcaaaagct tttatcagct gattttcaac cgttgattcc gatttccctg attctcgttc
     2281 ctccagaagc tcgccaacgc acagcactgg aatgatatca aatgctatcg ccgaaataac
     2341 ctttttgtgt atcagatcat cggtttcgcc aaatatatgc cgacgctcgg aatgtccaac
     2401 aagcacatag ccagcaccaa catctttaag catctgtggc gatagttccc cggtataggc
     2461 tccttccttt tcataaaaca tattttgtaa accaactttt accggagtac cttcgcatgc
     2521 tccaataaca caggttgcgt ataccgatgg agggaaaagc acaacctcac gatccatgaa
     2581 attcataccg ctggcaatac cgatggcaag ctcaaccgcc tcacgttttg tagtgtacat
     2641 cttccagtta ccggcaaata tctgttttcg tacctgttgc ttaatcatgg catcaccttt
     2701 tatttattat tgtaaataac tatcatacgt agtgttatcg caaattacac cgcaataaaa
     2761 attatgcaag cattttattg ctaccagcat gtctagcgta tcatatgtca tatgttactt
     2821 ctacatcgtg cctctgctga aattgttttt tcctttgtta agggtagcat tattctcatt
     2881 gatgttttcc aattagtgta aaatcccgta gaagtaccct aataatacat gacgatatca
     2941 caatgtgaat accataacat tccattcatc tttatgttta ttgcatattt tttacaggaa
     3001 aaaaagagta acctattata aaaaaaggca tgtcactgct gacagccttt tttttatagt
     3061 aactatctat tataatttta gtttatttca tacaatactg tatcagatca acaacacggc
     3121 aggaataccc ccactcatta tcgtaccagc ttaaaatctt aaccatgttg ccgccaataa
     3181 cagccgttga aggtgcatcc acaatagatg aatgattatt gcccttgaaa tcaatcgata
     3241 ccagctcttc atcgcatacc tgtaaaattc cttttagctt accatttgca gcttcccgca
     3301 aagccttatt cacatcctca gcggttgtct gagttttaac cgtacataca aaatcaacca
     3361 ctgatacatc aggggtgggg acacgaatgg acagcccatc aagcttacct ttgagctcgg
     3421 gcaatactaa agcaactgcc tttgctgcgc cagttgtggt tggaatcatt gaaagagctg
     3481 ctgcccgtgc tcttctgaaa tccgaatgag gaaggtccaa tagacgctgg tcatttgtat
     3541 atgaatgcac cgtggtcata agcccttttt caataccaaa tacatcatta acaactttag
     3601 caaccggcgc caaacagttt gtagtacagg acgcatttga gataatatta tgttttgctg
     3661 tatcatattt gtcttcgtta acgccaagga caacggttat atcttcacct ttagccggtg
     3721 cagtaataat taccttttta gcacccgcat ctaaatgcgg cattacctta tcacggtccc
     3781 tgaattttcc ggttgattca acgaccacat ctatactttc tgctttccac ggaagtgtag
     3841 ctggattttt ttctgccaat atctttattt ctttaccatt aatagtgatg gcattttctt
     3901 tatggcttac tgtcccgtta aaaacaccaa aaaccgaatc atatttaaan nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna taaaatgatt gatgcaagca gcaacctgat
     4081 gaacctgttc aatatgtgac atgtgtcctt tatagggcaa tacctgcaac attgcatcag
     4141 gcattttttt aaatgttgca tagatataat caagcggcac cattgtatcc ttacggttag
     4201 ctatgaagca cgatgtaaag ggaaactgtg ctgccgtttc atcatagtta tagccatcgc
     4261 atagtgtaaa atcatttttc agaacattac tgccagcctg cttcatacat tcaatagctg
     4321 ttttacgcat cgtatcatca atagcaccat agcttaaatc aacagcaagc ttacataacc
     4381 catcaaaatc tttatccaga agatcaaata caacaggatt taccggtaac tttccggctg
     4441 ttgctaaaaa tagtgctcct gctatcaaag cgttacgttg taccagttca aagattatac
     4501 caccacccat tgaatgccct aacacaaata cgttaccgtt gagtgagcca ataaactgtt
     4561 gcatagcctg cacatatata tccagtgaac attcattgat tgcagactta ccatgtcccg
     4621 gtaaatcaac cgcatacact gctatggttt gtaatgcatt catgagtgga ataaattgcc
     4681 gtgaatcacc tcctgctcca tggatacaca ccagaatatt ttcactttgt atctcattat
     4741 accggtagta catttcacca atggttgttg caaaatatcc tgttttcatg gcaattgttc
     4801 ctgttcatta ttacaatatg agcgatagtt actgcgtatg ggcaatgccg tattaataat
     4861 tgcaactgtt ggcatgattc tttgcccgga atactataat cattaaagtt tcagaattta
     4921 ccattatgca tacaattacc agtgcatgtc aaccaggatt taacgcatcg tgcgctttac
     4981 actgcgggag ccattactac aatggagcat atcatgaaat tgacaatctt tttaaagaac
     5041 acttctattg tttcaataca tttatatcta cgaacaggaa tatccattcc ctgctgtcag
     5101 catgccgtaa cgctcttaaa acaaataaca ttaccgccat gtgatgatga cgctacacac
     5161 tgtacgtttg ttgcatatac cgatacaagc ttcacaacaa taggttgtct gttatatcca
     5221 cactgttaca ggtttgatta ccggagcctg taaatggaaa atatatgcaa aacatttaac
     5281 tgtagaatca atacaccagc ttactatgaa accgttataa ccgcagcaac catctttcat
     5341 gattggtact actatagtat tgccatacac catacagcat ggataaacac cctacccagg
     5401 atgtgcatgg cataacgtta aacaccagaa gaaaatttca ttatagcccg tgtagccgga
     5461 cactctatgg gtattataaa ctgtaaaatg caaagtgtat agtgtaagat tgaagttgtt
     5521 caatggcaga tgtaagcata accctgcaat gattattaat tattatagtt acgatgtagt
     5581 tatcatcgca aaatctggaa cagtgttaca attaagccag caatccctat tgattgagtt
     5641 ataaacaatg caatcaacca tcgtattgaa gaagaatgtt tctcttcaat cttttctatt
     5701 aagtcagatc taagcttttc cagtgaaata gatgattcgg cacgggattt ttcaatatcg
     5761 attgataaat ccgctcgtac cttctctatc tcctttgata aatccgctcg taccttctct
     5821 atctcctttg ataaatccgc tcgtaccttc tctatctcct ttgataaatc tgctcttaca
     5881 tcctctatct cctttgtcaa tttcaattcc gtttctttca tatctttgcg gatttgctca
     5941 atttctctga taagagccag ctcggtgttt tttatgtcag agcgcgttgc aacttcattg
     6001 accttaataa tctgttctaa cgattcaata acatcagcca gaactcttgc ctttgtttca
     6061 tcatctttaa acgcctcata aattttaact gccaaaccca ttttgcatcc ccctatgcat
     6121 ataaaaccat aaatgaaact ctataggtat caatatagtt gcattgctaa caatgtcaaa
     6181 taaaaattat gacttgacta tctaaagtga tcatagtata atttttgtaa taatgaattg
     6241 cattacatat tataagttta taaaattgaa tctttcatta gcattatcat ggaaattttt
     6301 taaagaatgc atcacagtaa aaacagattt tgtattattt tgtaagctat aaacataatg
     6361 gagggagcta tgaatataac atccaactca tttgccagtg ggggaataat tccggtaaaa
     6421 tatgccatga aaggaatacc cggtggtcag aacatctccc cacatattgc aatacatgat
     6481 gtacccaaag aagctcgctc gcttgctgtt gccttcgttg accgtcatcc aatggcgcgc
     6541 aactgggtgc actggctggt gctcaatata cctgcacata ctaaagaaat tcaggaaggc
     6601 gcgtcactga aagctatgcc acatgattcc attgagatga tcaatacttt tggttttgca
     6661 ggttatggtg gacctcaacc acctaaaggt agcggtgtcc atagctatga actttgctgt
     6721 tatgcacttg ctgaattatt caagatacca aaaaaagaat taaccgaaaa tgaattgtta
     6781 aaactcataa aaccattgct gattacccag tgtacaatac acggtaactt tgaaaataaa
     6841 taacgttact ttggcagtac atgctcatgt atcataccat ataaaaagta ttgactataa
     6901 tatctttttc tgagtgttag tttttttatt gatataaatt tatacctcat gaggaatgct
     6961 atgggtatcc cggtttacca atacgataat atcattgaag ttcaaaaaga aggtgtgtgc
     7021 gatagctccg aaatcaatgc aatagctgct attgatgcac agttgaagaa tgatgaaaaa
     7081 atccgttcgc ttctcatcag tgaaacatgt attaaaaatc gaactgcagc acttgcaggt
     7141 gatatagttc gtgacatgaa tcattccaat catatcgata taatgatggt gctgacaggg
     7201 tcatttatgt ttactgctga tcttgcacgg gcactctatg cacagggtgg tattaataca
     7261 agcattcact gcatcaaaac aagtgtatac gatgaaacca taaaatcaag tggcgaacat
     7321 taccgggcag taacgctgga actatcgcca aaaaatatag aagcccgtga tattttgtta
     7381 gttgaagaca ttgccgatca gggatttaca ttatcatggc tcaagaatta tcttttatat
     7441 gagcggaatg cccgttcact caagatttgc agcttgctgt ataaacgtct ggataatccc
     7501 acacgggagg ttcagactat acgcaagcaa ttgggaattg attatattgg atttactgtc
     7561 cctgatgtat gggtagctgg ctatggcatt gacgccgcat atcaataccg gcatctgcca
     7621 ttcatagtgt ggatcaatga agcatacttt caatcatagg caaggtggct gtaccataaa
     7681 aaaatagttg ccaaaaaaac tttttaccat acgctgtaac ttcatccagt catctctaca
     7741 tgaattattt atacatacaa tttcagaaga gagcatgaca ttttcatttt ttagataggt
     7801 acaaaaaatt atcattgcat gtataatata tagtattata gaatactaac agaatagagt
     7861 taaggattaa tgatacaatg aaaaagttat tcactataac ggtactggtg tttattgttg
     7921 tatcttcatt atttgctttg acgggcgaag aagcaataat tcgatttcaa cagcggatgc
     7981 gttctattaa tacgctgagc gggaacattt cgtggacaac atcaaacgga tttacctaca
     8041 caggtacatt taaatataag gcaccggata aactttacct caaccttaca gcacccggtg
     8101 gcaaagcaat agtcaccaac ggcaaaaaat tatgggtata cgacaaatca tctaacattt
     8161 gcggagtaca ggatgttggc gacgggtatt caggaggcat tgcatcaatt gtatcaggat
     8221 ataatgcctt acttgcctcg cagggtactg gtggttatac tataaaatta cgaaattttg
     8281 aaaaagctta tccggaaata gtcattgtgg ctgatagtaa ttttttactt aagaagctta
     8341 tacttaaaga taaacagggc gattcaacca cctatacaat ttc
//
LOCUS       DAOOJH010000102         8355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7346_length_8355_cov_9.524458, whole genome shotgun sequence.
ACCESSION   DAOOJH010000102 DAOOJH010000000
VERSION     DAOOJH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7346_length_8355_cov_9.524458"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /gene="dnaA"
                     /locus_tag="PLJ75_10645"
     CDS             complement(<1..911)
                     /gene="dnaA"
                     /locus_tag="PLJ75_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPD05709.1"
                     /translation="MTNSTTIWNKVLGSVEAYINKQSFNMWFKNTEPVAFNEDVLVIK
                     VVDDVAKRHISDHYTSLILSIIKEQTGKDIICEFVTGNGFKDHIEETSSQKLHEQKET
                     LLFPTILNPKYTFENFVIGPNNQLAHAAAISVSKAPATQYNPLFIYGDTGLGKTHLMQ
                     AIGHRIIQERPYLNVLYVPCEQFINEFIQAIIANTLSSFKIKYRNVDILLIDDIQFIE
                     KKEQTQEEFFHTFNTLHNNKKQIVISSDRPPKDLSTLENRLRTRFEWGMIVDIQVPNL
                     ETREAILRNKAEKIKIELSDEVCNYIAR"
     gene            1254..1511
                     /locus_tag="PLJ75_10650"
     CDS             1254..1511
                     /locus_tag="PLJ75_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HPD05710.1"
                     /translation="MSVIIKDIQRNNTEIIRVEISEFKGKELINLRIWYQSIENGAVV
                     YKPTQKGVALSVDKFNELQEAVSKIAEYLHDKKFGTTPENV"
     gene            complement(1492..2850)
                     /locus_tag="PLJ75_10655"
     CDS             complement(1492..2850)
                     /locus_tag="PLJ75_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05711.1"
                     /translation="MIEKLDTTNQYIVVSFHKSIASENNFRIIISTSTPSVHHCILQY
                     LNDNSNTIALRLSLIVKHKLNITLISNHFLSKGENEVPNIEIKASIRDTDDRNSNNTF
                     SIYCENDFFKLFKINMDTSHRPVNTYELESQFLHFFKDPYTLFPSLPFILEVMSDDEF
                     QKLIYYLLNEKLLTPYHLFLISRAYPQYSLKIKHNISRHTISDIINVGKSIHKITYRD
                     VLEAIYAFEELLFLKLRSKPYFHYGTFIEQVHSILHHMRMVTLFQQKSFDEWVYEFIK
                     SNLLYAVLAKCDDITIAAAFYNNRHLISLLDNYISSKRINGIASLFEMECDTNLIISS
                     QHRMIELFLNTISSHNKLYSQPFNILLKKYMTPENMYYVLFEIGWFVIATALKQSSKK
                     LCLECIKHFPEGAQYCIIDVYNGVLNPNIVHDEMQIKKARNQLIHSLLKLNMYGIIHF
                     QE"
     gene            complement(2986..4140)
                     /locus_tag="PLJ75_10660"
     CDS             complement(2986..4140)
                     /locus_tag="PLJ75_10660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPD05712.1"
                     /translation="MSLLSQHNTTVPKNILVIDDSKLNRAIVKNTLLNYNIHVFEAAD
                     GLEGLTVLQSRHFDLILLDIVMPNMDGFGFLEQCKQYIGDDFIPIILMTGLDDLNSKI
                     KGLSIGADDFLLKPLNQKELIARVQSLLRLKQAHDELYEKNQQIQKELEYAKRLQQFI
                     IPKDFSFISYPSISGRYLPIADIGGDLFDCYKISDSKTGVLIADVTGHGIPAALVMSM
                     AKMTFNIHSYEIFPTQQLMSKINEEMRGLLLDMQYITAFYMLIDNEHKNIAFTNAGHT
                     RPLYYRKSTDKIIALDTNGLFLGIQDESLYEQKSIHYESGDRCILYTDGITELKDDHN
                     EEFGEVRLAKFLKQHNNASGSFCDNLLQTLEHFAPLNERSDDIAFLFIEF"
     gene            complement(4155..6356)
                     /locus_tag="PLJ75_10665"
     CDS             complement(4155..6356)
                     /locus_tag="PLJ75_10665"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD05713.1"
                     /translation="MTRLVIESMNGNYNQLFIVTTSEEAIRSIYLTFSGHPYTITITR
                     SGMELLSFISYQLPHLIVVGTDITDINAFELVSIIKKNSITRYVPCIVFGSHNELNDR
                     ISAIDSGADGYLTLPLKHEELQALVHAKLIQHDELYLLSVTDDLTGLFTRKEFFRRFT
                     FEIENKIHERFSIAIIDIDHFKNINDTYGHPIGDIVLIQLADILKSLQSNNHTPARFG
                     GEEFAILFPGLSSQEAKKIIDTIRLQFAGISFKTNIPDNSFSVSFSSGVAEYPTMGSN
                     CSELLSRADQALYSSKTDGRNRTYIFSPIMARNDKFWEYIKTNKTAKNIFIDPFLHEP
                     TTRFHYLPVVLENLLSSEISIDTIGILMIKALPLIDFYQYRGYKNFEYDIENLALLIP
                     KICELHFPYERHITIADLYDYEIAILFPFPSSLKTNTAEFNKLCKDLVLDISLQMVHQ
                     QIEIQYTHGIVPFHKNFAKNILHTIKAIRSHYTPLLSISNEKNIINDFSKAIDTKTET
                     GDYMFLLPVYHHATFKKTYYHVFSKQMVSANNYFDLMLKNGIVTSNDYLLFFNSLKDV
                     ILKQSLKVPLIINWTPRLDLKTQIDILQSLIATSAIPGLILAIDEIEMEEFLPQYEKY
                     SSFLDKNTIECAVSNCYIGSQLLHYLSTIELSMIILSDHIIRNIHYFKDRIKIINGLR
                     LFCDQINVPIYAPNILKEGEYKIIKDITVAYMSGPYIEQQFTLFSLNKKVV"
     gene            complement(6337..7179)
                     /gene="pyrF"
                     /locus_tag="PLJ75_10670"
     CDS             complement(6337..7179)
                     /gene="pyrF"
                     /locus_tag="PLJ75_10670"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPD05714.1"
                     /translation="MNYCESLQWYTQKYNSIVCMGLDPVIEDIPFFKDSIHKTIYAFY
                     EDILNNCIASHVFPSCVKPNFAFYAQYGMEGILALNDIIKLFHTEGFPVILDVKRGDI
                     GKTAQAYARESFEFFGADAVTLSPYLGYDSVEPFTKNYPDKGCYILVKTSNKSSADIQ
                     DIPVNGEPLYVLVAKKILEWHSPGMGAVVGATFPKQLKDIINIFVQSGKDIPVLIPGI
                     GTQGGDGDAVLDIIKNSEHTYLYRINASSSINYAYKNTEYNHVQYGVAAVEALKKLND
                     TFGH"
     gene            complement(7176..7715)
                     /locus_tag="PLJ75_10675"
     CDS             complement(7176..7715)
                     /locus_tag="PLJ75_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPD05715.1"
                     /translation="MNNYDTTILERIHIIQGDITKMDVDAIVNAANPSLLGGGGVDGA
                     IHRAAGPQLLEECRTLGGAKPGEAKITKGYRLPAKYVIHTPGPVYRGGNQHESTILKN
                     SYHNSMQLAKQYNCRSIAFPAISTGVYGYPKDEACRIAIDTVLDFMQKENFLIDVYFV
                     LFDDENFSLYINYLKELSK"
     gene            7860..>8355
                     /locus_tag="PLJ75_10680"
     CDS             7860..>8355
                     /locus_tag="PLJ75_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05716.1"
                     /translation="MIRYFDIVVVGAGHAGAEAALASARRGFSTLLVTGNLDTICQMS
                     CNPAIGGLAKGHLVKEIDALGGEMALAIDEAGIHYKMLNKSKGPAVWAPRAQADKKHY
                     QLRMKEVLEKQKNLTIVQDIVEKLMVEGNQIKGITTIRGQHHASQAVILCTGTFLKGL
                     IHIGN"
BASE COUNT         2725 a         1303 c         1492 g         2835 t
ORIGIN      
        1 cgagctatat aattacaaac ttcatctgat aattcaattt ttattttttc agctttattt
       61 ctgagtattg cttctcttgt ttctaaattt ggaacttgaa tatcaacaat cataccccat
      121 tcaaaacgag ttcgtaagcg attttcaagg gtagatagat cttttggagg tctatcgctt
      181 gaaataacta tttgtttttt attattatgt agagtattaa aagtgtggaa aaattcctcc
      241 tgcgtttgtt cctttttctc aatgaactgg atatcatcta ttaacaatat atccacattc
      301 cgatatttaa ttttaaagct tgatagtgta ttagcgataa ttgcctgtat aaactcatta
      361 ataaattgtt cacaggggac ataaaggaca ttaaggtatg gtcgttcctg aataatcctg
      421 tgcccaatag cttgcatcag atgcgtttta ccaagtccag tatcaccata aataaataaa
      481 ggattatact gcgttgctgg tgctttagaa accgagatag cagctgcatg agctaattga
      541 ttattaggcc caataacaaa attttcaaat gtatatttgg gatttaaaat tgttgggaat
      601 agcagtgttt ctttctgctc gtgtaatttc tgcgacgatg tttcttctat atgatcctta
      661 aaaccatttc cggtaacaaa ctcgcatata atatctttgc ctgtttgctc tttaatgata
      721 gaaagaatga gagatgtata atgatcagaa atatggcgtt tagcaacatc gtcaactact
      781 ttgattacaa gaacatcttc attgaatgct accggttcag tatttttaaa ccacatatta
      841 aatgattgct tattaatata agcttctacc gaacctaata ctttattcca gattgttgtt
      901 gaatttgtca taatacaaac ctgtttagtt attaacaatt gttaacaact ttgttgatat
      961 cgtaatatac gtttagcatg cggaaaaaag gttaactgag tgcatcagca taataactat
     1021 agtaactatg atactgtatt gtctctactt cagcataaag gttttttttt gcaaccaatt
     1081 aataaataaa attgatttaa cgaatgaatt tttttattat tatggatata gtgagaataa
     1141 gtcctgatat tgtgtgtata aaatgttaat aaatcaatat ttatagttga aaaatattaa
     1201 atcatgtgta ttaattgtta ataattttta cattataaaa aggaagtaac tctatgagcg
     1261 ttatcataaa ggatatacag agaaataaca ctgagattat acgagtggaa atatcagaat
     1321 ttaaagggaa ggaacttatt aatcttagaa tctggtatca atcgattgag aatggcgcgg
     1381 ttgtttataa accaacacaa aaaggagtag cactttcagt tgataaattt aatgagctac
     1441 aggaagcagt ttcaaaaatt gctgaatatc ttcatgataa aaaatttggt actactcctg
     1501 aaaatgtata atgccataca tgtttagttt cagcagggag tgtattagtt gattccttgc
     1561 tttttttatc tgcatttcgt catgtacaat gttaggattt aaaacaccgt tatagacatc
     1621 aataatacag tactgtgcac cctcaggaaa atgttttatg cattctaaac ataatttttt
     1681 agaagattgt tttagtgcag ttgctataac aaaccatcct atttcaaata atacataata
     1741 catgttttca ggtgtcatat atttctttaa tagtatattg aatggctgag agtacagttt
     1801 attatgagag cttattgtat tcaaaaagag ttcaatcatt ctgtgctgtg atgagataat
     1861 cagattagta tcacattcca tttcaaaaag ggatgctata ccgttgatcc tttttgatga
     1921 aatatagtta tccagaagtg agatcaaatg tctgttattg taaaaagctg ctgcaatggt
     1981 tatatcatca cattttgcaa ggactgcata taaaagattg gattttataa attcatacac
     2041 ccattcatca aaagactttt gttgaaatag tgttaccatt cgcatatggt gtaaaatgct
     2101 atgaacctgt tcaatgaacg ttccataatg gaaatatggt ttgctacgta atttcaaaaa
     2161 taaaagttct tcaaatgcat atattgcttc gagaacatcc cggtatgtta ttttatggat
     2221 tgatttaccc acattgatga tatctgaaat agtatgcctg gatatattat gttttatctt
     2281 cagtgaatat tgagggtatg ctctggaaat aaggaaaaga tggtaaggag ttagtagttt
     2341 ttcatttaat aaataataaa taagcttttg gaattcatca tcgctcatca cctcaaggat
     2401 aaagggcaat gaaggaaata gtgtataggg atctttgaaa aaatgtaaaa attgtgattc
     2461 aagttcatag gtattgacag gtcgatgaga ggtatccatg tttattttaa aaagtttaaa
     2521 gaaatcattc tcacaataaa ttgaaaaggt gttattgctg tttcgatcat ctgtatcccg
     2581 gatagatgct ttaatctcaa tgtttggcac ttcattttct cctttcgaca agaaatgatt
     2641 gcttattagt gtaatgttta atttatgttt cactatcaaa ctcaaacgca gggcaatagt
     2701 gttgctatta tcgttaagat actgcagtat gcaatggtgt actgatggtg tgctggtgga
     2761 tattataatc cggaaattgt tttctgatgc tattgatttg tgaaaagata ctacaatata
     2821 ttgatttgtt gtatccaatt tttctatcat gaaaggtgat aatgctaaac ctgaaaggtt
     2881 aaaataactt tttagataag catcgatctt atggaacagt atagaaaata taatatgaga
     2941 agcggcatta taatacatgt aaattagttt aataatttta ttatattaaa attcaataaa
     3001 taaaaaggct atatcatcac ttctctcatt taaaggtgca aaatgttcta atgtctgaag
     3061 aagattgtca caaaaagaac ctgatgcgtt attatgttgt tttaaaaatt ttgccagtct
     3121 gacttcacca aattcttcat tatggtcatc ttttagttca gtaatcccat cagtgtataa
     3181 aatacatcta tcacctgatt cataatgaat tgatttttgt tcataaagtg attcatcctg
     3241 aatgcctaaa aaaagtccat tggtatccaa tgcaataatt ttatcagtac ttttgcgata
     3301 gtaaagaggg cgagtatggc ctgcattggt gaaagctata tttttgtgtt cattatctat
     3361 gagcatataa aatgctgtga tatactgcat atctaacaaa aggccacgca tttcttcatt
     3421 tatctttgac atcagttgtt gtgtagggaa tatttcatat gaatgaatat tgaatgtcat
     3481 ctttgccatg ctcatcacca gtgcagcagg gatcccatgc cctgtcacat ctgcaattaa
     3541 tactccagtc tttgaatcag aaattttata gcaatcaaag agatcgccac ctatgtcagc
     3601 aattggaaga taccgaccac tgattgacgg ataagaaata aatgaaaaat ctttaggtat
     3661 gatgaattgc tgcagccgtt tggcatattc caattctttt tgtatctgtt gatttttttc
     3721 gtataattca tcatgagctt gtttcagcct caataatgat tgtacacggg caatgagctc
     3781 tttttgattc aatggcttta aaagaaaatc atcagctcct attgataagc ctttgatctt
     3841 tgagtttaaa tcatctaaac ccgtcatcag aataatagga ataaagtcat ctccaatgta
     3901 ttgtttgcat tgttctaaaa aaccaaaacc gtccatgtta ggcataacaa tatcaagaag
     3961 aattaaatca aaatgccgtg attgcaaaac ggtaagtcct tctaaaccat ctgctgcttc
     4021 aaaaacatgg atattataat ttaatagagt attttttact attgctctgt ttagtttact
     4081 gtcgtcgata accagaatgt ttttcggtac tgttgtgttg tgttgtgata gtaaagacat
     4141 aatggcaaca cctcctaaac caccttttta tttaaggaaa aaagagtgaa ctgttgttca
     4201 atatatggac ctgacatata ggcaacggtg atatctttga ttattttgta ttcaccttct
     4261 tttaaaatat tgggtgcata tatgggcaca tttatctggt cacagaataa acgtaatcca
     4321 ttgataattt taattctatc tttaaaataa tgtatattac ggataatatg atcagaaagt
     4381 attatcattg ataattcaat agtagataga tagtggagca attggctgcc aatgtagcaa
     4441 ttagatacag cacattcaat tgtattttta tcaagaaagg atgaatactt ttcatattga
     4501 gggagaaatt cttccatttc gatttcatct attgcaagga ttagaccggg aattgcagaa
     4561 gtagcaatta aagattgtaa tatgtcaatt tgtgttttga ggtctaatcg tggtgtccag
     4621 ttgataatta aaggaacctt taaagattgt tttaaaatca catcttttaa tgaattgaaa
     4681 aagagtaaat aatcattaga tgttacaata ccatttttta gcattaaatc aaagtaatta
     4741 tttgcagaaa ccatctgttt actaaataca tggtaataag tttttttaaa ggtggcgtga
     4801 tgatataccg gcagcaaaaa catgtaatca cctgtctcgg ttttggtatc tatcgctttt
     4861 gaaaaatcat tgattatatt tttttcattt gaaatagaca gaagtggtgt gtaatgagat
     4921 ctaatagctt taatggtatg taaaatattt ttagcaaagt ttttgtgaaa tggcacaatt
     4981 ccatgtgtat attgaatctc tatttgttga tgtaccattt gtaatgatat atccagaacc
     5041 aggtccttac acaatttatt aaattccgct gtatttgtct ttaaagaaga gggaaaaggg
     5101 aaaagtattg ctatttcata atcatataga tcagcaattg ttatatgccg ttcatagggg
     5161 aaatgtaact cgcaaatctt tgggataagt aaagctaaat tttctatatc atattcaaag
     5221 tttttatatc ctcgatattg ataaaaatca ataagaggca gagcttttat cattaaaatt
     5281 ccgatggtgt ctatagagat ttccgaagat agtagatttt ctaaaacaac cggtaaatag
     5341 tggaatcgcg ttgttggctc atgaaggaat ggatcaataa aaatattctt tgcagttttg
     5401 ttagttttta tatattccca gaacttatca ttacgagcca taataggact gaaaatgtag
     5461 gttctatttc tgccatccgt ttttgaagaa tagagagcct ggtcggcgcg tgacagcaat
     5521 tctgagcagt ttgaacccat ggtgggatat tctgccactc ctgaactgaa gcttactgaa
     5581 aaagaattat ctggaatatt tgttttaaaa ctaatacctg caaattgaag acgtattgta
     5641 tcaatgattt ttttagcttc ctgagaagaa agtccgggaa atagtattgc aaattcttca
     5701 ccaccaaaac gtgcaggggt atggttattt gattgtaaag attttaaaat atcagccaac
     5761 tgtattaata caatatcacc aataggatgg ccataagtat cattaatatt tttaaaatga
     5821 tcaatatcaa tgatagcaat tgaaaaacgc tcatgaattt tattttctat ttcaaatgta
     5881 aaacgtctaa agaattcttt tcttgtaaaa agtcctgtta aatcatcagt aacagacaat
     5941 agataaagtt catcatgttg aatcagctta gcgtgaacta aagcttgtaa ttcctcatgt
     6001 ttcaatggta atgtcaggta tccatcagca ccagaatcaa tggcagaaat tctgtcgttc
     6061 aactcattgt gagatccaaa aacaatgcag gggacatagc gcgtaattga gtttttctta
     6121 atgatgctca caagctcaaa agcattgata tcggtaatat ctgttccaac tacaattaaa
     6181 tgtggcagct gataggaaat aaaacttagt agttccatgc cacttctggt aatagtgata
     6241 gtgtatggat ggccagaaaa agttaaatat atggacctga ttgcttcttc acttgttgtc
     6301 acaataaaaa gttgattata gttaccattc attgattcaa tgaccaaacg tgtcattaag
     6361 tttttttaat gcttctactg ctgctacacc atactgtaca tgattatact ctgtattttt
     6421 ataagcgtag tttatactgc tggatgcatt tatccgataa agataggtat gctcactatt
     6481 tttgatgata tccagcacag catcaccatc tccgccctgt gtccctatac ctggtatgag
     6541 caccggaatg tctttaccgg attgcacaaa gatgttaatg atatccttga gttgttttgg
     6601 gaatgtagcg ccaacgacag cccccattcc tggcgagtgc cattcaagta ttttttttgc
     6661 aaccagcaca tataagggtt caccattcac tggtatgtcc tgtatgtcag cagagctttt
     6721 attggatgtt ttaacaagga tatagcaacc cttatcggga tagtttttgg taaatggttc
     6781 tacagaatca tatccaaggt aaggggatag cgttacagca tcagcaccaa aaaattcaaa
     6841 cgattccctt gcataggctt gagcagtttt acctatatcg ccccttttca catccagtat
     6901 taccgggaat ccttcagtgt gaaatagttt aatgatatcg ttcaaagcca gtatcccttc
     6961 cataccatac tgagcataaa aggcaaagtt tggttttacg cacgatggga aaacatggga
     7021 tgcaatacaa ttgttaagta tatcttcgta gaatgcatat attgttttgt gtatagaatc
     7081 tttgaaaaat gggatatctt caatcactgg atcaagaccc atgcatacta tactgttata
     7141 tttctgtgta taccactgca aactttcaca ataattcatt tcgatagttc cttaagatag
     7201 ttgatatata gagaaaaatt ttcgtcatca aaaagcacaa aatatacatc tattaagaaa
     7261 ttttcttttt gcataaaatc aagaactgta tcaatggcta tcctgcacgc ttcatccttt
     7321 ggataaccat agacaccagt agagatggca gggaaggcaa tagatctaca attatattgt
     7381 tttgccagtt gcatgctgtt gtgatatgaa tttttgagta ttgtagattc atgctggttg
     7441 ccccctcgat acacaggtcc gggagtatgg ataacgtatt ttgcaggaag tcggtagcct
     7501 tttgtaatct tagcttcacc aggtttggcg ccacccagcg ttcggcattc ttctaatagt
     7561 tgtggcccgg ctgcacgatg aatagctcca tcaaccccac ctccaccaag taaagatgga
     7621 tttgcagcat tgactatggc atcaacatcc attttggtta tatcaccctg tatgatatgg
     7681 attcgttcca gtatggttgt atcatagtta ttcataataa ctccaatcat ttgtgtaaac
     7741 gattaaaaaa tatttgtaat cacccatttt acgatattat atttatagta tacaaaaata
     7801 tgtagcaatt gcaagcatat tattggaaaa ttaaaagtaa gggcgatagt taccatacaa
     7861 tgatacgata ttttgatata gtagttgtcg gtgcgggcca tgctggtgct gaagcagctt
     7921 tagcttcagc acggcgtggt ttttcaacac tattggttac cggtaattta gatactatct
     7981 gccagatgag ctgtaacccg gctatcggcg gattggcaaa aggacatctg gtaaaagaga
     8041 ttgatgcatt aggtggtgag atggcactgg cgatagacga ggccggaata cattataaga
     8101 tgctcaacaa aagcaagggc cctgctgtat gggctccgcg agcgcaggct gataaaaaac
     8161 actatcagtt gcgtatgaaa gaggtattgg aaaagcagaa aaatttaaca attgttcagg
     8221 atattgtaga aaagctaatg gttgaaggaa atcaaattaa aggaattaca acaatacgag
     8281 gacaacacca tgcttcccag gcagttatcc tgtgtaccgg gacattctta aaggggctga
     8341 tccatatcgg caatt
//
LOCUS       DAOOJH010000103         8329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7367_length_8329_cov_10.068044, whole genome shotgun sequence.
ACCESSION   DAOOJH010000103 DAOOJH010000000
VERSION     DAOOJH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7367_length_8329_cov_10.068044"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /gene="lysS"
                     /locus_tag="PLJ75_10685"
     CDS             complement(<1..1481)
                     /gene="lysS"
                     /locus_tag="PLJ75_10685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPD05717.1"
                     /translation="MADDINDLVLQRIEKVKQLRTKGINPYPIRYKPTHTAQAIKEQF
                     GEEDKRLVTAAGRIKSKRVMGKASFAHIEDASGLIQVYTRLDRLGEEKYELFKTLDLG
                     DIIGVAGHVFKTKQGEITIEVDDVVLLAKSIRPLPVVKEKDGKVFDEFADKEQRYRQR
                     YVDLIVNPQVRQQFILRSNMITGIRNFLNQKGFIEVETPMMHAIPGGAAARPFITHHN
                     ALDIDLYLRIAPELYLKRLLVGGFDRVYELNRNFRNEGISTRHNPEFTMLELYQAYAD
                     YNDMMAITQELISTLAKELLGAIVITYQGNTIDLTPPWQKLRYSEALKKYTGIDFTAI
                     SRDEAYAEAVKRNIDVTKEMSLWKIAEEFFEEKVEENLIQPTFIIDYPRELSPLAKSR
                     EDNPEFVERFEVFIAGREMANAFSELNDPFDQRQRFEEQVKMREAGDDEAQMMDTDYI
                     TALEYGMPPAGGLGIGIDRLAMLFIDTASIKDTILFPLLKPEK"
     gene            1804..2628
                     /locus_tag="PLJ75_10690"
     CDS             1804..2628
                     /locus_tag="PLJ75_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05718.1"
                     /translation="MNHLFKTILHIIIGIICCCIVTNNSYSAEKLFSFPVGVTYDSIY
                     WWRGVELNGKGAGVIWPKVGCEVSGVQGYITIGINQDYYMTTEGGDNDVAKTYHEFDY
                     GLEYSFTQNTFSSTIGIKYCQYPFYDSAGATVDPSFFEAYGILSLSCVIIPVLELYYD
                     YYREESDEETPVNEDVYAKLSLFKEILHANSFIVKTGIWLGYYHNAYLDRDGFSDAGI
                     TLSTSYDYNSVVFFSAVNYARTLSEDFYIEYDADANGHASTLKNHLWVDIGISSKL"
     gene            2776..3411
                     /locus_tag="PLJ75_10695"
     CDS             2776..3411
                     /locus_tag="PLJ75_10695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014955693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPD05719.1"
                     /translation="MDNYLKVSALKRFIFTIKTYLYWQLSYRKKFASIAGNPVIWGLW
                     NIDIYGPNIHLGKNVVFLAANGSKTALTTVQLAGHEGRIDIGNNVLIMNGVRISSASH
                     VRIGDDCMLANFCYLTDADWHDIHDRTNPVGKTAPIILDNGVWIGDSAIICKGVRIGQ
                     NSVVGAGAVVTRDVPSNVVVAGNPARIVKEIDPEKVITHSTLYKKVGTPRL"
     gene            3408..4643
                     /locus_tag="PLJ75_10700"
     CDS             3408..4643
                     /locus_tag="PLJ75_10700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD05720.1"
                     /translation="MRICLLCYRANPYCGGQGIYLKYVAEALSRQGHEVHAIVGPPYP
                     QPMDNVTVHYIDNNQYYIKKGFDIISRNSPFDIFHPINSYECIHSRFGAFPEISSFSY
                     RAFFAVRKLHEQFQFDIIHDNQCIGYGLLFMKALGIPVIATIHHPLTIDLENVLERGS
                     SFKNKIKGVMFYPVLMQQIVSKRLDHIITVSEDSKRRIASDFGVPLHKQSVVYNGLDT
                     TIFKPLPHIKKKAKKILFVGNVEDGKKGFVYLLKALTLLKTSVTLTVIDGGAPHRRIT
                     QALVDTLGIRDIIEFAGTTSTQQLVEHYNESAITVVPSVYEGFGFPAAEAMACGTPVI
                     ASDGGALREVVGDAGIVVPSRDEVSLASAIDNLLGNAKLRQELSHKGIERVKTHFNWD
                     SASAKMVALYKQVIATHQR"
     gene            complement(4634..>4991)
                     /locus_tag="PLJ75_10705"
     CDS             complement(4634..>4991)
                     /locus_tag="PLJ75_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05721.1"
                     /translation="GRIPIIKNYNSAKAIKQSNGESFILLENDQMHQTISMKENVPDK
                     IMFVSKENSKRYEIYFIKPAVTNTLFSYKGLRLVDVQSKTAFEITMQKYSINEPIPDD
                     ITDLKLPKNTKIIDYR"
     assembly_gap    4992..5091
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5092..6634)
                     /locus_tag="PLJ75_10710"
     CDS             complement(<5092..6634)
                     /locus_tag="PLJ75_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05722.1"
                     /translation="MKKILSALLLIVISCSSLPTSETKKDNIHYKETALELYSKALLL
                     KNEKKYTEAIALLAKASENTEQRDAIYYQMAECYFYQYNYEKAIEYADKAIAANDSFD
                     KPYILQYSVYMNLNQAEDATQVLEKLLIKRPQLYRIRFNLASVYYSQTKQNLKARYHF
                     LKIIEQSQYETIESYYKEQSYYFLGHIYYGFGEYKKAYQFFLKAYNENPDNYSILYIL
                     GLLNLEKGDIKQATVYLSQYLERYPNDIKAILHLGRIYYIYEDMKALPVLRKAINHKS
                     SEGTLAKMMYTEMLHNDDKALELAQKLRRMNSSLDMPYIALARIAMRNNDSSTAATNY
                     YTAGVLLYQKHHYDAAISMFVNALHSDAAIHDAYVYLGQIYEDTNQLQLAIYYYTMAY
                     KLKKNNDIMIRIGYIYTLLKDYTRAFEYFTMATSPDHTNPDVYFFKGLALLQKSDYAN
                     AGDMFRKALKIKRAEPYYFYLASALDKQKKIHDTISVLQEAYNNDMKSARICNYLGYL
                     YADNNM"
     gene            6880..7578
                     /locus_tag="PLJ75_10715"
     CDS             6880..7578
                     /locus_tag="PLJ75_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPD05723.1"
                     /translation="MLFGIDPLYWMMMAPVLLISLWASFRVKSAFNKYSQIPSRSGYT
                     GGDVARIILQRNGLSHIPVEETTGFLSDHYDPIKKVVRLSPQVYNSNSLAAIGVAAHE
                     TGHALQHAKAYKPLVLRNAIAPTASIGSNLSWIIIFAGFIIGSLGLVKFGILLFSLVV
                     FFQLITLPVEFNASTRAKEILAGYGIVSSGELAGVSKVLSAAAMTYVAAAASAIMTLL
                     YFLIRAGFLGGSDD"
BASE COUNT         2392 a         1612 c         1562 g         2663 t
ORIGIN      
        1 tatttttccg gcttcagcaa cggaaacaat atcgtatctt ttatggaagc ggtgtcaata
       61 aacaacatag caagccggtc aattcctatg ccaagtccac cagcaggtgg cattccatac
      121 tcaagtgctg ttatatagtc ggtgtccatc atctgtgcct catcatcgcc ggcttcacgc
      181 atctttacct gctcctcaaa acgctgccgc tgatcaaacg gatcattgag ctcgctgaat
      241 gcatttgcca tttccctgcc tgctatgaaa acttcaaagc gctcaacaaa ctccggatta
      301 tcctccctgc tttttgcaag tggcgatagt tccctgggat agtcgatgat aaaggttggc
      361 tggatgagat tttcctcaac cttttcctca aagaactctt cggctatctt ccacaatgac
      421 atctctttcg ttacatcaat attacgcttc acggcttcgg catatgcctc atcgcgcgaa
      481 atggcggtaa agtcaatccc tgtatatttt ttcagtgcct cactatagcg tagcttctgc
      541 cacggcggcg taaggtcaat ggtattgccc tgataggtaa taactatcgc ccccagaagt
      601 tcctttgcca atgtgcttat tagctcctgt gttattgcca tcatatcgtt gtaatcagca
      661 tacgcctgat acagctctaa catggtaaat tcaggattgt ggcgcgtcga tatgccttca
      721 ttcctgaaat tgcggttaag ctcatacacc ctgtcaaatc cgccaacaag cagacgcttt
      781 aaatacagtt caggggcaat acgtaaatat aaatctatat caagtgcatt gtgatgcgtt
      841 ataaaaggac gtgcagccgc accacccggt atcgcatgca tcatgggtgt ttcaacctct
      901 ataaagcctt tttgattcag gaaattgcga atgccggtaa tcatgttgct gcgcagtata
      961 aattgctgtc gtacctgagg atttacaatt aaatcaacat aacgctgtcg gtatcgctgc
     1021 tccttatcgg caaactcatc aaacaccttg ccatcttttt cctttaccac tggtaacgga
     1081 cgaatgcttt tggcaagcag cactacatcg tcaacctcaa tggtaatctc accctgcttt
     1141 gttttaaata catgccctgc aactccaata atatcaccta aatcaagcgt tttaaacagc
     1201 tcgtattttt cttctccaag cctgtcaagc ctcgtataca cctgaatcag tccgcttgca
     1261 tcttcaatgt gggcaaagct tgccttaccc atcacgcgct ttgactttat ccttccggct
     1321 gccgtaacaa ggcgtttgtc ctcttcgcca aactgttcct tgattgcctg cgcagtatgt
     1381 gttggtttat atcggatagg gtatggattg attcctttag tgcgcaactg ctttactttt
     1441 tcaatgcgct gaagtactaa gtcgtttatg tcatctgcca ttatgggata cctcataaaa
     1501 ataaactatg cctgtaatgt agcacaaacg gtttgttttc tctattttgt gcgatagtta
     1561 cctgcaaatg ttactggatg atactagaga agcaattgct gtcaattgaa aatttgtttg
     1621 atagtattta tcctgatcat cccttttttg ttacataaag attacaggta tatttcatca
     1681 atgtaacatt ttctataatg atgagatgaa taatactctc atattgacac cattaacttg
     1741 atacaattaa aaatataaag acgcacataa catgcgtctt tatattttta aaaggaggta
     1801 tgtatgaacc atttattcaa aaccatttta catattatta taggtataat ctgctgctgt
     1861 attgttacaa ataatagtta ttcagcagaa aaattatttt cttttcccgt aggggtaacg
     1921 tatgacagta tctactggtg gcgtggtgtt gagctaaacg gtaaaggtgc aggcgttata
     1981 tggccaaaag ttggttgtga agttagcgga gtacagggat atataactat agggataaat
     2041 caggattatt atatgaccac tgaaggtggc gataacgatg tagcaaagac atatcatgaa
     2101 tttgattatg gacttgaata ttcatttact cagaatacct tcagcagcac tatcgggata
     2161 aaatattgcc agtacccatt ttatgatagt gctggcgcaa cggttgatcc atcttttttt
     2221 gaagcgtacg ggatattgag tctttcctgc gtcattattc ccgttctgga actatattat
     2281 gactactatc gtgaagagtc cgatgaagaa acacctgtca atgaagacgt ctatgcaaaa
     2341 ttatcactgt ttaaagagat actgcatgca aacagtttta ttgttaaaac cggtatatgg
     2401 cttggatatt accataatgc ctaccttgac agagatggtt tcagtgatgc gggaataaca
     2461 ttgagcacat cctatgatta taatagtgtt gtattttttt cagctgtaaa ctatgcccgt
     2521 acactttcag aagattttta tattgagtac gacgcagatg ccaatggaca tgccagtaca
     2581 ttaaaaaacc acctctgggt tgacattggc atcagcagca aactgtaata gcataaccaa
     2641 aatcactatg tcacttttta aaggagtgag gctacaacta tcactccttt tgtttatgaa
     2701 ccatagaaaa aaaactcttg acgatgaatc aaatttgaat agggtgcaca tagcttaacc
     2761 cggaaaggac atactatgga caattatctt aaagtttcag cattgaaacg atttattttt
     2821 actataaaga cctatttgta ctggcaacta tcgtaccgta aaaaatttgc atccattgct
     2881 ggcaatcctg tgatctgggg attatggaat attgatatat atggaccaaa cattcatctg
     2941 gggaaaaatg ttgtcttcct tgcggctaac ggcagcaaaa ccgcccttac caccgtacaa
     3001 ttagcaggac atgaagggcg catcgatata ggcaacaatg ttcttattat gaatggtgtt
     3061 cgtataagca gcgcctccca tgtcaggata ggcgatgatt gcatgcttgc caatttctgc
     3121 taccttactg atgctgactg gcacgatatt catgaccgta ccaatcctgt tggcaaaaca
     3181 gcccccatca ttttagacaa tggcgtctgg attggcgatt ctgctataat ctgtaagggt
     3241 gtgaggattg gacaaaattc agtagtgggg gctggtgcag tggtaaccag agatgtgcct
     3301 tccaatgtgg tggtggcagg caaccctgcc cgtattgtaa aagagattga tcctgaaaag
     3361 gtaataacgc acagtacact gtataaaaaa gttggaacgc cacgcctatg aggatatgcc
     3421 ttttatgcta ccgagctaat ccttactgcg gagggcaggg aatatacctt aaatatgtag
     3481 ccgaggcgct atcacgccag ggacatgagg tgcatgctat tgtgggcccg ccctatcctc
     3541 agcctatgga taatgtaact gtccattaca tcgacaataa tcaatactat atcaaaaaag
     3601 gatttgatat catctcccgc aacagcccgt ttgatatatt tcatcccatc aacagttatg
     3661 aatgcataca ttcacgattt ggtgctttcc ctgagatcag cagcttcagc taccgggctt
     3721 tctttgcagt gcgcaagctt cacgaacaat ttcagtttga tatcatccat gacaatcagt
     3781 gtattggcta tgggctcctt ttcatgaagg cattgggcat cccggtaata gcaaccatac
     3841 atcacccgtt aaccattgat ttagaaaatg ttcttgaacg cggatcatcg tttaaaaaca
     3901 agataaaagg tgtgatgttt tatcctgttc tcatgcagca gattgtatca aaacggcttg
     3961 atcatattat aaccgtttcg gaggattcaa aacgcaggat tgcctctgat tttggggtgc
     4021 cactgcataa gcagtcggta gtgtacaatg ggctggatac tacaattttt aagcctttac
     4081 cgcatataaa aaagaaagca aaaaaaatac tttttgttgg caatgttgaa gatggtaaaa
     4141 aaggttttgt gtacctgctc aaggctctga ccctcttaaa aaccagtgta acattaaccg
     4201 ttattgatgg cggagcacca catcggcgta ttacccaggc gctggtggat acattgggca
     4261 tacgcgatat catagaattt gcaggaacaa catcaacaca acagcttgtg gagcactaca
     4321 atgaatcggc gataactgtt gtgccatcag tttatgaagg atttggcttt ccggccgcag
     4381 aagcaatggc atgtggaaca ccggttattg caagtgacgg tggcgcactc agggaggttg
     4441 tcggtgatgc cggcatagta gtcccttcac gcgatgaggt tagtttagcc agcgcaatag
     4501 acaaccttct gggcaacgca aagcttcgtc aggaactatc gcacaaaggg attgaacgag
     4561 taaaaacaca ttttaactgg gatagcgctt ccgcaaaaat ggttgctctc tacaaacagg
     4621 ttattgctac ccatcagcga taatcgataa tctttgtatt tttgggcaat tttagatcag
     4681 taatatcatc agggatgggc tcattgatgg aatatttctg catggtaatc tcgaaagcag
     4741 tttttgactg tacatcaaca agccgtaacc ctttatatga aaaaagggta ttggtgacag
     4801 ctggttttat aaaataaatt tcgtagcgtt tactattttc ctttgaaaca aacataattt
     4861 tatcggggac attttctttc atggatatcg tttgatgcat ctggtcattt tccagaagta
     4921 taaaactttc accattgctc tgttttattg ctttcgcaga attataattt ttaatgattg
     4981 gaattctacc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcatattat
     5101 tatcagcata caaatacccc agatagttgc atatcctggc acttttcata tcattgttat
     5161 atgcctcctg taacacactg atggtatcat gtatcttttt ttgtttgtcc agagccgatg
     5221 ctaaataaaa ataatacggt tcagccctct taatttttag tgctttacga aacatatccc
     5281 cggcattggc ataatcagac ttttgtaata aggcaagtcc tttgaaaaag taaacgtcag
     5341 gattggtatg gtcaggggag gtagccatag taaaatactc aaatgctctg gtgtaatcct
     5401 taagcaaagt atatatataa ccaattctta tcatgatatc gttatttttt ttcaatttat
     5461 acgccatggt atagtaataa atagctaatt gcaactgatt ggtatcttca tagatttgtc
     5521 ccagatacac gtaagcatca tgtattgcag catcgctatg caacgcattg acaaacatgg
     5581 aaatggctgc atcataatgg tgtttttgat ataacaacac accagcagtg taataatttg
     5641 tagcggcagt actgctatca ttgtttcgca ttgcaatacg ggcaagagca atataaggca
     5701 tatcaagcga actgttcatt cttctgagtt tttgggccag ttccagggct ttatcatcat
     5761 tatgaagcat ctcggtatac atcatctttg caagagtacc ctcagagctt ttatgattga
     5821 tagccttacg tagtactggc agtgccttca tatcttcata aatataatag atgcgtccta
     5881 aatgcagtat tgcttttata tcattgggat accgctccag atattgcgac aggtacacag
     5941 tagcctgttt tatatcacct ttctctaaat ttaataatcc cagaatatac agtatggaat
     6001 aattgtctgg attttcatta tatgccttca aaaaaaactg atatgctttt ttgtattcac
     6061 caaaaccata atagatatgt cctaaaaaat aatatgattg ttctttgtaa tatgattcaa
     6121 ttgtttcata ttgactttgt tcaattatct ttaaaaaatg gtagcgtgct tttaaatttt
     6181 gtttagtttg tgaataataa acacttgcaa ggttaaaacg tatccggtat agctgtggcc
     6241 gttttattaa tagcttttcc agcacctgag ttgcatcctc agcctgattc agattcatat
     6301 ataccgaata ttgtaatata tatggtttat caaagctatc attggcagca attgctttat
     6361 cagcatactc aattgctttt tcataattat actggtaaaa atagcattcc gccatctggt
     6421 aatagattgc gtcgcgttgt tcagtatttt ccgaagcttt agcaagcagg gcgatagctt
     6481 ctgtatattt tttttcattt tttaaaagca gtgccttgct gtataactca agcgcagttt
     6541 ctttatagtg gatattgtcc ttctttgttt cggaggtcgg tagtgatgaa catgatataa
     6601 caataagcaa taatgctgaa aggatttttt tcatggtgtt gattcctgcc ttacataaat
     6661 caataaactg tatactacga taccgtacaa tatactgcgt tgatggttag tgtcaaatat
     6721 aaaacttgca aatattgtga cgttatgtat tattttgtaa caaaatataa ttttaagata
     6781 caaaaatact ttgcagttac tattttttta tgtattttgt atattgactg tgtatacatt
     6841 aattactata atttaatgat aacaatgagg aggtagtaca tgctttttgg catagatcca
     6901 ttatactgga tgatgatggc tcctgttctt cttatatcac tatgggcttc attcagagtg
     6961 aaaagtgcat ttaacaaata ttcacaaatt ccgtcacgtt ctggctatac tggtggagat
     7021 gtagcacgta taatattaca gcgaaatgga ttatcacata taccggttga agaaaccacg
     7081 ggttttttat ccgatcacta tgatccaata aagaaagtgg tccgtctgtc accacaggta
     7141 tataacagca attcattagc tgccattggt gttgctgctc atgaaacagg gcatgccctc
     7201 caacatgcta aagcttataa acctttagtt ttacgaaatg ctatcgctcc aactgcttct
     7261 atcggctcca acctatcatg gattatcatc ttcgcaggat ttattattgg ttcacttggc
     7321 ctggtaaaat ttggcatact gcttttcagc cttgtggtat tttttcagct tattacattg
     7381 cctgttgagt tcaacgcttc aacacgtgca aaagaaatct tggcaggata tggtatagtc
     7441 agttcaggtg agcttgcagg agtaagtaaa gtactatcag ctgccgcaat gacctatgtt
     7501 gccgctgcag cttctgctat tatgacactg ttgtactttt taatcagggc agggttttta
     7561 ggtggcagcg acgattgatt attttaatta atttttgata gctctataat aaaaacagta
     7621 tatcgggttg aaccaatatg ctgttttttt ataggtttac tttaattaca aggccataca
     7681 gtatacctat ccttcctcca cttttctgct tctggcattt tctttttttt tactgccatc
     7741 ttcacatttt atattgtatc atctcatata acgttatggg gcaagcaatg tcccgataaa
     7801 gacgttttat ggcttagcct gaagagaatg ggttcctttt tcttcattaa tttttctgga
     7861 tcaggtaaaa aatccctgat tacttccgtt gttacaataa taaatttcac tctttccgga
     7921 ttattgtaac cacccagata gaatttttat ataaaaaata tttactataa atttaataac
     7981 attatttcgg taagctatat atgtatggtt ataatgcatc agaaaatttc atgatcagtc
     8041 agtattttta gtggtactga atagttccat gttattggaa gttttattgc aacacctaac
     8101 agcaaaattt taatggcatt aatgcgcatg ccaacactct caaaataccg tatcacctat
     8161 aaaggcaata tcatatacca gagcagtttt tttattaatt gttgcacgac cattgcaata
     8221 gaacatacca aacttccacc cgcgtaaaac tgttgttaca gcagtatttt acttgttgac
     8281 atatctaaat tattcattat atacagttat aaatacatca ggatatttt
//
LOCUS       DAOOJH010000104         8328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7368_length_8328_cov_8.481929, whole genome shotgun sequence.
ACCESSION   DAOOJH010000104 DAOOJH010000000
VERSION     DAOOJH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7368_length_8328_cov_8.481929"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PLJ75_10720"
     CDS             <1..677
                     /locus_tag="PLJ75_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05724.1"
                     /translation="KNNNKILTFMVYSSMLLTKRLVTMKTSKDKIIHAAIIEFANYGL
                     DGARVDRIAKKAKINKAMIYYHFKNKEALYADIVKNLAQTIFDTVQGSLQKAIEAGNP
                     IIVIEDYARHMSSLDKHYFLIIMRELSSGGKFIRKIVAPTIIEPITELVGKFYQLGNN
                     KNMLKDIHPQYTMISIAGAVIFYNLMRQILEGTSVYQKYFQESNVEQYIANLLQLLAH
                     GIVKKE"
     gene            680..2074
                     /locus_tag="PLJ75_10725"
     CDS             680..2074
                     /locus_tag="PLJ75_10725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD05725.1"
                     /translation="MKMICITRLLSLIIILFISIPVNALQKLTLNQAIDIALKNNFEL
                     LTAQQRLKQANEIIYQVWGMLYPALESEADAIRQYAENGFLSLSDGQYDLKFIQIKFG
                     INPGNFYNTLTQSYSNYKLAKEDVKKITSTIEYNVIKSYFDVILADEVITLRKNSINV
                     LQSNLLVVQNLFNTGTVPKFELLQAQVQLQSQEPLLIEAANNYNTALDLFNYHVGAKT
                     GTYTIAYEGFDSTIKKPQPVAIVNLLVEKALLYRPEVLQLSLRKDMVKDSIEATQSVY
                     LWPTFSIAGYYGKTYLLPNTSDVPLPFPGQSLDLSTITGNKEWQTTWQIRFAATYRWG
                     SLVPVDTVQGVEREEKEKLKEIEERIKQVKQSIIIAVRTDYGKLITAYETIIAQNKNI
                     ETASEGLRIAKESYRAGVIKNSDLLNAELQLTSARMGYIKAINDFYCTIAQLKKDTGF
                     DCTEIIFTEEMYEK"
     gene            2064..2861
                     /locus_tag="PLJ75_10730"
     CDS             2064..2861
                     /locus_tag="PLJ75_10730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPD05726.1"
                     /translation="MKNKRIIPVIVVVVIVVGVTIYFEMLRYSGKNDNRIEGSGVIEV
                     TEVEVASKIAGRVVELPFDEGEEVKQGALIVKLAYDELSAQKLSALANLTNAEKNLKR
                     ARELFASGSISQRELDNAVAAYNVAKGNYDFVMATIDQAVLYAPVAGVVLTRNLEVGE
                     LALPGSSIMTLADLTKAWIKIYVNERYIGRVKHGQKAFVYTDSYPDKPFPGKVVAIAN
                     KPEFTPKTIQTKEERVKLVFAVKITIDNSNKELKPGMPADAVIVTGE"
     gene            2861..3772
                     /locus_tag="PLJ75_10735"
     CDS             2861..3772
                     /locus_tag="PLJ75_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD05727.1"
                     /translation="MIQVKGLIKQFNSIIAVNDVSLQLEKGELVGIVGPDGAGKSTLL
                     RIMAGILQPDIGTTLFGNKPYQDAIYHVKEHIGYMPQRFGLYEDLTVLENIMFFGKIF
                     GLREKEIKKTLPVLWNFSNLEAFKDRLAGKLSGGMKQKLGLACCLVHKPAILLLDEPT
                     NGVDPVSRREFWKILYDLCVEGVTIVVSTAYLDEAERCNRVALMYHGSFLKVDTPTGI
                     KAMINKPLLEIVTNRPVEAEQILKTNPEYMHALRSGNAVRVFVDNVAMSLPAIEKLLF
                     SHKISVLTIHKTTPSLEDIFVEAIAPR"
     gene            3775..4914
                     /locus_tag="PLJ75_10740"
     CDS             3775..4914
                     /locus_tag="PLJ75_10740"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD05728.1"
                     /translation="MISLLRIIAIAQKEWIHIYRDIRSLILSLFTPALLVLIFGYALN
                     MDVKNVTFVIYDQNRTSFSRQFAEIFMHSEYLQYSGYVHSFHDIDAIINSNKAVAALV
                     IPFDFTKKLKSGKSSQVQLLLDASDSMSATIASGYFNAIISQYNLQLQHNALNSLGYG
                     NSITPISVETRIWYNEELESKNFVVPGIIVIVLAIISALITSLTISREWERGTMESLI
                     TTPVRSYQIIIGKLLPYIFIGIFDVVITYLVGYFVFDIPIRGSFAELMLVSTLFLIGT
                     CTFGLVISSATRIQVLSIQVAMIATYLPTILLSGFIFPIKNMPIILQAITYIIPARYM
                     VSLIKGIAIKGLAASLLSTQIIFMVIFALVMVIVGIKKINLQYIG"
     gene            4919..6064
                     /locus_tag="PLJ75_10745"
     CDS             4919..6064
                     /locus_tag="PLJ75_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD05729.1"
                     /translation="MKLSLNNIKIMIRKEFTLVIRDTNFRAIIIVPPILMTLVFGYAV
                     NTDVNDVLFSVYDRDRTAQSRIFIQQFVASPYFALHSYIDSPKQGMTLIEKGDTDFYL
                     EIENGFSKALKTGKPVALQVILDGTDSSRASVIQSYINSITLSYIQEQFISIIKMKIL
                     LQSQIGLQQSFIELIPRYWFNQDLKSVNFYLPGVIAMLIALITIMLTSMSIVKERETG
                     TMEQIKVSPLKPQEYMIGKLVPFAIIAFIDIIMVTAIAIFWFGIPFRGSFVFLLLSGI
                     IYIVSTLAIGLFISSVSSTQQQAMLSSLMFFFPSILLSGVIFPIYSMPKVIQLVTYLN
                     PMRYFAEIVRGIFLKGSGLYYLWHNIIILLVMSIVILRISIWQFGKR"
     gene            6073..6987
                     /locus_tag="PLJ75_10750"
     CDS             6073..6987
                     /locus_tag="PLJ75_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD05730.1"
                     /translation="MYTVIVNNITRRFGDFTAVNAISFSVSKGEIFGLLGPNGAGKST
                     TIKMLCGLLQPDDGVGTVAGYDIVKQQWEIKNSIGYMSQKFSLYEDLSIEENLKFYGT
                     IYGLGTAQLKQRMHYIMEIVDIISHRHTVVKNLPPGIKQRLALGTALIHDPQILFLDE
                     PTSGVDPLMRRIFWDEIYKLANEGKTVFVTTHYMDEAEHCNRIALIIAGKIIALDTPQ
                     NLKENLPYKIIKITTNKFLQAYDILGNIHEIIEVALFGTDIHIVVDKNFASTATIKAL
                     LLSKGIESKLMPILPTLEDVFVMHARTV"
     gene            complement(7085..7897)
                     /locus_tag="PLJ75_10755"
     CDS             complement(7085..7897)
                     /locus_tag="PLJ75_10755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05731.1"
                     /translation="MLIATMYDDVTQIKMSREVDGKGIYWVAAYLIDGLLVDTGCMFT
                     AQELLDFLKDKEILQVVNTHFHEDHVGANKLLQEQRGVAIYAHPDSIPLIASKQFLYP
                     YQEFVWGYPEPSVTAPVPALIKTNKYTFSVIETPGHSAGHISLIERSRGWCFSGDIYS
                     RVKVKFIRPEEDVADCIASMGKIMSMLTERLVLFTSVGKIVEDGRQELSDTINYFNEL
                     AGQVAILLQKGCDTNTIINTLFGGEHSFADLTNGQYTTRNLVNSLITMVKNN"
     gene            complement(8096..>8328)
                     /locus_tag="PLJ75_10760"
     CDS             complement(8096..>8328)
                     /locus_tag="PLJ75_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05732.1"
                     /translation="SVVTRLERLGYVQKKISNDDKRLTLISITRKGKKYKPVLLELSR
                     AMNKKCFYGISKDEKAMLFKILQKIYENADRL"
BASE COUNT         2687 a         1561 c         1522 g         2558 t
ORIGIN      
        1 ggaaaaataa taataaaata ttgactttta tggtatatag cagtatgttg ttaactaaac
       61 ggttagttac tatgaaaaca agtaaagata agattattca tgcagctata atagagtttg
      121 ctaactatgg tcttgatggt gcacgggttg accgtattgc aaaaaaagct aagataaaca
      181 aagcaatgat atactaccat tttaaaaaca aagaagcgtt atatgctgac attgtaaaaa
      241 atttagccca aaccatcttt gatactgttc agggaagtct gcaaaaagct atagaagcag
      301 gcaatccaat catagttatt gaagattatg caaggcacat gagcagcctt gataagcact
      361 attttttgat cattatgcgt gaactttcaa gtggtggtaa atttatacgg aagatagttg
      421 ctcctaccat tattgaacca ataacagagc ttgttgggaa attttaccag cttggaaaca
      481 ataaaaatat gctaaaagat attcatccgc aatataccat gatttccatt gccggcgctg
      541 ttatctttta taatctcatg cggcagatac ttgaaggaac ttcagtctac caaaaatatt
      601 ttcaggaatc taatgttgaa caatatattg caaatctgct gcagctttta gcgcatggta
      661 ttgtgaaaaa ggagtaatta tgaaaatgat atgtattact cgattactat cgctcataat
      721 aatacttttt atttccattc cggttaatgc tctgcaaaag ctaactttaa atcaggcaat
      781 tgacattgcc ttaaaaaaca actttgaatt attaacagcg caacagcgtt taaagcaagc
      841 caatgaaata atttatcagg tgtggggtat gctttacccg gccttagagt ctgaagctga
      901 tgccatacgg caatatgcgg aaaatggatt tttaagttta tctgacgggc agtatgattt
      961 aaaatttatt cagattaagt ttggcataaa tccggggaat ttttacaata cattaactca
     1021 aagttatagt aactacaaac tggcaaaaga agatgttaaa aaaataacat caactattga
     1081 gtacaatgtc attaaaagct actttgatgt tatactggct gatgaggtta taacattacg
     1141 taaaaattct atcaatgtgt tacaatcaaa ccttcttgtt gtacaaaacc tttttaatac
     1201 cggtaccgtt ccaaaatttg agctgctgca ggcacaggtt caattacaaa gtcaggaacc
     1261 tctacttatt gaagcagcaa ataactataa cacagcgctt gatcttttta attaccatgt
     1321 tggtgctaaa acaggaacat atactatagc atatgaagga tttgatagca ctattaaaaa
     1381 acctcagcca gttgccattg ttaatttgtt ggttgaaaag gctcttttgt accggcctga
     1441 ggtgctgcaa ctgtcattac gtaaggacat ggtaaaagat agcatagaag caacccaatc
     1501 ggtgtatctg tggccaacat tttccattgc ggggtactat ggtaaaactt accttttacc
     1561 caacacatcc gatgttccac ttcccttccc cggtcaatct cttgatttgt ctacaattac
     1621 tggtaacaaa gaatggcaaa caacatggca gatacgattt gcagcaacgt atcgctgggg
     1681 ctcgcttgta cctgttgata ccgtacaggg agtggagcgt gaagaaaagg aaaagcttaa
     1741 agaaattgaa gaaagaatta aacaggttaa acaaagtatt attattgctg tacggacaga
     1801 ttacggcaaa cttatcactg catatgaaac aatcatagca caaaacaaaa atatagaaac
     1861 agcttcagaa ggtttacgta ttgctaaaga aagctatcgt gctggagtta taaaaaattc
     1921 agatttgtta aatgcagaat tgcagcttac ctctgcacgc atgggttata tcaaggcaat
     1981 caatgacttc tattgtacta tcgcccaact taaaaaagac acgggatttg actgtactga
     2041 aataattttt accgaggaaa tgtatgaaaa ataaacgcat tattcctgtt attgtggtag
     2101 tggttattgt tgttggcgtc accatctatt ttgaaatgct gcgatatagt ggcaaaaacg
     2161 acaaccgcat agaaggttcc ggcgttattg aagtaaccga ggttgaggta gcatctaaaa
     2221 ttgccggcag agttgttgag cttccttttg atgaagggga ggaggtaaaa cagggagcac
     2281 ttattgttaa attagcatac gatgaactga gcgctcagaa actttcagca ctggctaacc
     2341 ttaccaatgc tgaaaaaaat ttaaaacggg cacgtgagct ttttgcaagc ggctctattt
     2401 cgcagcggga acttgataat gctgttgccg catacaacgt tgcaaaagga aattatgact
     2461 ttgttatggc aactattgat caggcggtgt tgtatgcacc tgttgcaggt gttgtgctta
     2521 cacggaattt agaggtaggc gagcttgccc tccccggcag ttccatcatg acgttagctg
     2581 atttaacaaa agcatggata aagatatacg ttaatgaaag atatattggc cgggtaaagc
     2641 atgggcaaaa ggcgtttgtg tataccgata gttatcctga caaacccttt cccggaaagg
     2701 tggtggctat tgccaataag cctgagttta caccaaaaac tatccagaca aaagaggaac
     2761 gcgtgaaatt agtctttgct gtaaaaataa ctattgataa cagcaataaa gaattaaaac
     2821 caggaatgcc tgcagatgct gttatagtaa caggtgaata atgatacagg ttaagggact
     2881 cattaaacag tttaacagca ttattgcagt taacgatgtt tcattacaac tggaaaaagg
     2941 tgagcttgtt ggtattgtag ggcctgatgg tgccggcaaa agcacattgc taagaatcat
     3001 ggctggcatt ttgcaacctg atataggtac aacacttttt ggcaataaac catatcagga
     3061 tgctatctat catgttaaag agcatattgg ttacatgcca caacgttttg ggctatatga
     3121 agacctaacg gtacttgaaa acatcatgtt ctttggcaaa atatttggat tgcgcgaaaa
     3181 agaaattaaa aaaacattac cggtgctctg gaatttcagc aatcttgaag cgtttaaaga
     3241 tagattagcc ggaaagcttt caggtggcat gaaacaaaag ttggggctgg catgctgtct
     3301 tgttcacaaa cctgcaattt tattgctgga tgaaccaacc aatggtgttg accctgtttc
     3361 acggcgtgag ttctggaaga tactatatga tctgtgtgtg gaaggagtaa ctattgtagt
     3421 cagcaccgca tacttagacg aagccgaacg ttgcaaccgt gttgccctga tgtaccatgg
     3481 ttcattttta aaagtggata ctccaacagg tattaaagca atgatcaaca aaccattact
     3541 tgaaattgta accaatagac ctgttgaggc tgaacaaata ctaaagacaa atcctgaata
     3601 tatgcatgca ctccgcagcg gaaatgcagt gcgtgtgttt gttgacaatg ttgcaatgtc
     3661 tttgcctgct attgaaaaac ttttattttc acataaaata tcagtgctca caattcataa
     3721 gacaacgccg tcattagaag acatctttgt tgaagcaatt gcaccgaggt gaacatgata
     3781 agtcttttgc gcattattgc gatagctcag aaagaatgga tccacatata ccgtgatatt
     3841 cgaagcctta tactatccct ttttactccg gcattattag tgcttatatt tggctatgct
     3901 ttgaatatgg atgtaaaaaa tgttacattt gttatctatg accagaacag aacatcgttc
     3961 tcacgccagt ttgcagaaat atttatgcac agtgaatatc tgcagtatag tgggtatgtc
     4021 catagctttc acgatattga cgccattatc aacagcaaca aagccgtagc agcgctcgtt
     4081 atcccttttg attttactaa aaaacttaaa agcggcaaat catcacaggt tcagttgtta
     4141 ttagatgctt cagattccat gtctgcaacc atagcatcag gatactttaa tgcaattata
     4201 tcgcaatata atctgcaatt acaacacaat gcattaaaca gcctgggata tggcaatagt
     4261 ataacaccaa tttcagttga aacacgcata tggtataacg aagaattgga aagcaaaaat
     4321 tttgttgtcc ctggcattat tgtgattgtt cttgccatca tcagtgctct tataacatca
     4381 ctcaccattt cacgcgagtg ggagcgagga actatggaaa gccttataac aaccccggtg
     4441 cgctcatatc agattattat tggcaaacta ctcccttata ttttcattgg catctttgat
     4501 gtcgttatta cctaccttgt tggatatttt gtcttcgata tacccatcag aggaagtttt
     4561 gccgagctca tgttggtttc tactctcttt cttattggca cctgcacctt tggacttgtt
     4621 atctcatcgg caacacgaat tcaggtactt tcaatacagg tagctatgat tgccacctat
     4681 ctgcctacga tacttttatc aggatttatt ttccctataa aaaatatgcc catcatttta
     4741 caggcaataa cctatatcat tcctgcccgc tacatggttt ctcttatcaa aggtatagct
     4801 ataaaagggc tggctgcatc acttctttca acacagatca tcttcatggt catctttgca
     4861 ttagttatgg tgatagttgg cattaaaaaa ataaacttac aatatatagg atagttacat
     4921 gaaactatca ctgaataaca taaaaattat gataaggaaa gaatttacac ttgtaatacg
     4981 agatactaat tttagagcaa tcatcattgt cccacccatc ctgatgacat tggtatttgg
     5041 ctatgcggtc aatacggatg tcaatgatgt tttattttca gtatatgatc gggataggac
     5101 agctcagagc agaattttta ttcagcaatt tgttgcatcg ccatattttg ccctgcattc
     5161 ttatattgac tcgcctaaac agggaatgac ccttattgaa aagggtgata cagactttta
     5221 tcttgaaata gaaaacggat tttcaaaagc tttaaaaacc gggaagcctg ttgcattgca
     5281 ggttatcctt gatggaaccg attccagccg tgcatcagtt atacaatcat atatcaattc
     5341 cataacactt tcatatatac aggaacaatt tataagtatt attaaaatga agatattatt
     5401 gcaatcacaa atcggtttgc aacaaagctt tatagaactt atcccacgct actggtttaa
     5461 tcaggatttg aagagtgtaa acttttacct cccgggcgtt atagcaatgc tcatagcact
     5521 tatcactatt atgctcacct ccatgtccat agtaaaagaa agggaaactg gaacaatgga
     5581 gcagattaaa gtatcaccgc ttaaacctca agaatatatg attggcaagc ttgttccctt
     5641 tgccattatt gcctttattg atattataat ggttactgcc attgcaattt tctggtttgg
     5701 catcccattc agaggaagct ttgtgttttt attattatct ggtattattt acattgtctc
     5761 tacgttagct attgggctgt tcatatcatc ggtttcatca acacaacagc aggcaatgtt
     5821 atcatcactc atgttctttt ttccgtcaat tttattgtca ggtgtgatat tccctattta
     5881 ttctatgccc aaagtcatcc agcttgttac ttatttaaat ccaatgcgct attttgcaga
     5941 aatcgtcagg ggaatatttc ttaaagggag cggtttatat tatctatggc acaatatcat
     6001 cattcttctg gtgatgagca tagttatttt acgtatcagc atatggcaat ttggcaaaag
     6061 gtaaccatat ttatgtatac agtaatcgta aacaacatta ccaggcgttt tggcgatttt
     6121 acagcagtga atgcaataag tttttctgtt tcaaagggtg aaatatttgg actgcttgga
     6181 cctaatggtg ccggtaaaag cactaccata aagatgttgt gcggattatt acaacccgat
     6241 gatggcgttg gtactgttgc cggctatgat attgttaaac aacaatggga aattaaaaac
     6301 agtattggat acatgtcgca aaaattttca ctgtacgaag atttaagcat agaagaaaat
     6361 ttaaaatttt atggaaccat ttatggactg ggcactgcac aattaaaaca acgcatgcac
     6421 tatatcatgg agatagttga tattatatct cacagacaca cggtggtaaa aaatcttccg
     6481 cctggaataa aacagcgact tgcattaggt acagctttaa ttcatgatcc acaaatttta
     6541 tttctggatg aaccaacatc aggagttgat cctcttatgc gcaggatatt ctgggatgaa
     6601 atttataagc ttgctaacga ggggaaaaca gtatttgtaa caacccacta catggatgaa
     6661 gcagaacatt gtaacagaat agctctgatt attgcaggta agattattgc acttgataca
     6721 ccacaaaatt taaaagaaaa tctgccatat aaaataataa aaatcaccac caataaattt
     6781 ttacaggcgt atgatatctt agggaatata catgaaataa ttgaggttgc tctttttgga
     6841 accgatattc atatcgtagt agataaaaat tttgcatcaa ctgctacaat caaagcactt
     6901 cttttgtcaa aagggattga atcaaaatta atgccaatac tcccaacatt agaagacgtc
     6961 tttgtaatgc atgcgcgaac agtgtaataa caacttcttt tctaaacagt atatttttaa
     7021 tttcatgata atgtatagct tgaaaaactc attcactata actataacaa gcattaacaa
     7081 aattttagtt gtttttcacc attgttatca atgaattcac cagattgcgt gttgtatatt
     7141 gtccattggt taaatccgca aagctatgct cgcccccaaa caacgtattg ataatagtat
     7201 tggtatcaca tcctttttgc aataagattg ccacttgtcc agcaagctca ttaaaataat
     7261 taatagtatc cgatagctct tgccgtccat cctctacaat ttttcctacc gatgtaaata
     7321 ataccaaccg ctcagtcagc atggacataa tctttcccat agaagcaata caatcagcaa
     7381 catcttcttc cggccgaata aattttacct tgactctgga atagatgtca cctgagaagc
     7441 accatcctct ggacctttca attaacgaga tatgtccagc actatggcca ggtgtttcaa
     7501 tcactgaaaa tgtatactta tttgttttaa tcaatgcagg aacgggcgcc gtgacagatg
     7561 gctcaggata gccccaaaca aattcctggt atggatacaa aaattgcttt gatgcaatca
     7621 gtggaataga atcaggatga gcataaatag caacaccacg ttgttcttgc aataatttat
     7681 ttgcaccaac atgatcctca tggaagtgcg tgttcaccac ctgcaatatt tctttatctt
     7741 tcaaaaaatc aagcaactcc tgtgctgtaa acatacatcc cgtatcaacg agcagcccat
     7801 caattaaata cgctgcaacc caataaatac ccttcccatc cacttcgcgt gacatcttaa
     7861 tctgggttac atcatcatac attgttgcta tcaacatgta ttaccactct taccaacata
     7921 gaaatattaa cctcaccccc ggcccctctc cttcgcaagg cgagaggagc atttgttccg
     7981 gtttgcatac ttttcatccc ctatgagcag ccataaaccg gccatcatga aatcaaaaca
     8041 atacattgga aacattatca ctgatccaac aatttattaa tacaaaaagt tgtaattata
     8101 acctgtctgc attttcatat atcttctgta atattttaaa aagcattgct ttttcatctt
     8161 tactgatacc ataaaaacat tttttgttca ttgcacggga tagttctaat aacactggtt
     8221 tatacttttt ccctttacgt gttatactta ttaaggttaa acgtttatca tcgttactta
     8281 tttttttctg tacatatcct aacctctcaa gcctggttac aaccgatg
//
LOCUS       DAOOJH010000105         8225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7461_length_8225_cov_12.359608, whole genome shotgun sequence.
ACCESSION   DAOOJH010000105 DAOOJH010000000
VERSION     DAOOJH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7461_length_8225_cov_12.359608"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLJ75_10765"
     CDS             <1..374
                     /locus_tag="PLJ75_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05733.1"
                     /translation="QFYLQRRLAMLNIKVNQLADVVIIDLEGEFDIDVIRMVETAWNE
                     AVKKKPSVIGFNCKDLKFIDSSALGTLVKFMNSASNKKINFTLFNVAPSIVSILKTSR
                     LDKFFTINSKEDFERKFMQNV"
     gene            500..1759
                     /gene="leuC"
                     /locus_tag="PLJ75_10770"
     CDS             500..1759
                     /gene="leuC"
                     /locus_tag="PLJ75_10770"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPD05734.1"
                     /translation="MGMTITEKILAAHAGKEFVEPGELIEARVDLLLGNDITAPIAIK
                     EFEELGINTVFNNEKIALIPDHFTPNKDIKSAEQVKILRDFAHKFAITHFYDVGRVGV
                     EHALLPELGLVRPGMCIIGADSHTCTYGAFGAFSTGVGSTDMAAAMATGKTWFKVPES
                     IKFIVHGILQPYVSAKDVILHIIGDVGVDGALYRSMEFVGDTIAKLSMEGRMTMANMA
                     IEAGAKNGIFAPDGKTVEYIKSRTNLPYTLYYSDDNAHYAEVREYDAATIEPTVAFPH
                     LPENTKPAREAHIPIDQVVIGSCTNGRIEDLRIAAKILKGHKVHKHVRLLIIPATQEV
                     YKKALQEGLIDIFIEAEAAVSLPTCGPCLGGHMGILAEGERCVATTNRNFVGRMGHPK
                     SEVYLAGPAVAAASAISGKIVHPDELR"
     gene            1792..2286
                     /gene="leuD"
                     /locus_tag="PLJ75_10775"
     CDS             1792..2286
                     /gene="leuD"
                     /locus_tag="PLJ75_10775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPD05735.1"
                     /translation="MKAHGRAWKFGNDINTDEIIPARYLNTSDPGELALHCMEDADPD
                     FMKKAQAGDIIVAGKNFGCGSSREHAPIAIKAAKISCVIAHSFARIFYRNSINIGLPI
                     IESEEASVDIEQGDEIAIDFDSGIIKNITKNKEYTAKPFPPFMQDIIARGGLMAKIKA
                     EIAQ"
     gene            complement(2288..4381)
                     /locus_tag="PLJ75_10780"
     CDS             complement(2288..4381)
                     /locus_tag="PLJ75_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPD05736.1"
                     /translation="MKPAKIVKYSAIVLSIVLFVIAALMAAFVYLFPRELLKSIIVET
                     LQKSLNRPVSIGTIDYSLRGIQITDLYIYDTDNNKPFIAHAKDSAIRFQLLPLLQKQF
                     VINYIYLNNGAIHIIYYTENKIFTSNLAKLIHELLAKEESSIQTQIESIAIENTAIIL
                     ENPPEVLKPLEGRYSISTLCKLNESTIDLHNLSITMPQNRGKVTGDCAISKKGNFSIR
                     GDVVLHACDLLWVYGWSRHDLASILPYRIFSGTVSNLLITTHHVQGTATGSCKLASNA
                     MIYITNGFCNVDIDKKKVAIANVKGKVENSTFVLNQLIFNFDGDIQSIKVTDADIKLR
                     EIAPLLGNIDISSINADITGNASYTGKMIDADVTVSNVTIGKNGTILSINDKVTLIIK
                     NNQCKKDAIPAQVYSIPCVLSIATVDTSFKKFIINAEIQELNLNAPDITNIPGTGKSK
                     KEIAIPVELSGKITIHTLIKDKLAINNINCSYIMSRNIISIPSFTAKIFGGDIAGRSI
                     ITLHPENPQINLVATFNTIRLQNISSYVEKYENRLFGNASGQLQVTIVPKEPLLDRII
                     GKIEFTIDNGKLANTGLQNGLSIWLADLKYKLSNLEFQKIYGNFHLNGPAITINSFIF
                     NAPAIRIKLNGKYHRHDESDIDIALEFSSQFIQDLPNPTLLQLAKYKKGRWYIIPFKA
                     KGTDILNGKNIKQVQ"
     gene            complement(4359..5576)
                     /locus_tag="PLJ75_10785"
     CDS             complement(4359..5576)
                     /locus_tag="PLJ75_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD05737.1"
                     /translation="MVQEQFKVVNYLANSFIVVEGKRNADNFYIIRQGKVKIVKENPV
                     AQESNQLLGPGDFFGVIPCMSGHAHIEGAVAMTNVSLISVQRDQFGILIQKNPAVAMK
                     IIRFFSRKLREFDQAITRLTFANAVEEDPEHLFNIGQYYLKKKNLPHAAYALQRYIQH
                     CADGINRDKAITYLKSINAPFKVPENPQKNSLTRVYKDNQMIFCENEPGDELYIIQGG
                     KVKITKIVDEEVLLAVLKPGDIFGEMALLENRPRSASAITFGDTSLMAINRQNFETMV
                     QTQPQLATRLIQLLSERIWTAYRQLENLMIRDPLGRMYDTLLIQVEKQKIRITPKESY
                     TFDFGVKELLNMVGISHDKGDYLVVELLEDKNITLDEGKLICTNLEELEKTVNFYKKK
                     SALERKREASKNS"
     gene            complement(5807..6157)
                     /locus_tag="PLJ75_10790"
     CDS             complement(5807..6157)
                     /locus_tag="PLJ75_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05738.1"
                     /translation="MIEHVQSNSVKPVAGQFHKQKPSIITSNTDVLAPMKRFKKDLPI
                     PKMGATASSSMQKVLINQKEFYSHRGQVYEQLRNPRAAIASYQKATYYEPSDTNLSSH
                     IIKLKVKSLTNLKA"
     gene            complement(6338..8014)
                     /locus_tag="PLJ75_10795"
     CDS             complement(6338..8014)
                     /locus_tag="PLJ75_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05739.1"
                     /translation="MIPLLITSLAIVIVILIWAITGNDTPQAKSESSPEKIYRRRASD
                     LAIEEAYPDGTKPKRRATDTPPERTDIDEDFKLPYTAEEIIPPYSRFRIYIRILMNAE
                     VYAKKGDFPTAISLYEGVNERILDAETNSKIDANINYLKKYRHKQEQQQRQRETVAKT
                     TPNEIKISLDSPIRLPEKIQIGFAPQDVDINNIIDEITKRILEKQRLQNTEDTAISNY
                     SNEIKQLSSHLEELKKEVASSLKELSSKEDALKTSEIQKLQNQLNDLTDRMFKLYEEK
                     EKTRAELENLKTVHSSLLEGSKQPAVSTVKYEGKLPIELDPEPILKILEKLPKIAAPA
                     SKPIDKDRMKGDSAKDFLKKLYNDTVKQGETIQSSEIVEKTEKLKEDEEWELISDIIK
                     DEKKSQLDEISDEDIFKKILEEDKRKRADDIEIVGDYRKTDDVEYDIQDTETLHKLEE
                     DRKFYEKFLQHDRRKKKELPILRVTYDFKKLPDEFSLSREKNILEYAFYKYRPLLQKA
                     DELIQKRKVRDALNYYRVVMQQNIPPEFKSMIRKNIADLNEYLEKYLTSD"
BASE COUNT         2364 a         1475 c         1603 g         2783 t
ORIGIN      
        1 aacaattcta tttacagagg agattggcaa tgctaaatat taaggtcaac caattagctg
       61 atgtagttat aatcgatctt gaaggagagt ttgatatcga cgtcatacgc atggttgaaa
      121 cagcatggaa tgaagcagta aagaaaaagc catctgttat aggatttaat tgtaaagatc
      181 tcaaattcat agactcttca gcattaggca ctcttgttaa atttatgaac agtgcatcaa
      241 acaaaaaaat aaactttaca ctatttaatg tggcaccttc tattgtttca atcctcaaga
      301 catcccgact ggataaattt tttacaataa attcaaaaga agattttgaa cggaaattca
      361 tgcagaatgt atagctatct atagaattgc atcatcgtat ataaaaaaaa tgaaaatata
      421 taaaagaatg atacaattct cataatgtaa agtatatgtt ggtatagtat gttactttaa
      481 ccacgttggg gatgatagta tgggcatgac aattacagaa aaaatattag ctgcacatgc
      541 agggaaggag tttgttgaac caggtgaatt gattgaagcc agagtagatt tactgctggg
      601 gaatgatatt acagcaccta ttgcgataaa ggaatttgaa gaattaggga ttaatacagt
      661 atttaataat gaaaagattg ctctgatccc ggatcatttt actcctaaca aggatattaa
      721 atctgcagag caggttaaaa tactgcgtga ttttgcacat aaatttgcta ttacccattt
      781 ttatgatgtt ggacgggtgg gtgttgaaca tgctttattg cccgaattgg gattggtccg
      841 tccggggatg tgtattattg gtgctgattc gcatacatgc acctatggag cttttggtgc
      901 tttttctacg ggtgttggct ccactgatat ggcagcagct atggctaccg gtaaaacatg
      961 gtttaaagtc cctgagtcaa taaagttcat tgtacacggt atattgcagc cctatgtatc
     1021 ggctaaagat gtcatcctgc atatcatcgg tgatgtgggc gttgatgggg cattgtaccg
     1081 ttcaatggaa tttgttggtg atactattgc aaagctctct atggaaggca ggatgaccat
     1141 ggctaacatg gcaattgaag ctggtgcaaa aaacggtata tttgctccag acggtaaaac
     1201 agtggaatac atcaaaagtc gtacaaacct tccctatacg ctgtattata gtgacgacaa
     1261 tgcacactat gccgaggtga gggaatatga tgcggcaact atcgaaccta ccgtcgcatt
     1321 tcctcatctt cctgaaaata caaagccagc acgtgaagca catataccca ttgatcaggt
     1381 ggtcataggt tcgtgtacca acggtcgcat tgaagattta cgtattgctg caaagatatt
     1441 aaagggtcat aaagttcata aacatgtgcg tttgcttata ataccagcaa cacaggaagt
     1501 atataaaaaa gctcttcaag aggggctgat tgatatattt attgaagcgg aagctgcggt
     1561 ttccctgcca acatgcgggc catgtttggg ggggcacatg ggaatcttag ccgaaggtga
     1621 acgctgtgtt gcaacaacca atagaaattt tgttggcagg atgggacatc caaaaagtga
     1681 ggtttacctg gcaggtcctg ctgttgctgc tgcatcagca atatcaggga agattgttca
     1741 tcccgatgaa ttgcgttgac taacattact acatggtaaa cgaggtacac tatgaaggca
     1801 catggtagag catggaagtt tggcaatgat ataaatactg atgagattat accagcccga
     1861 tatttaaata catcagatcc aggcgaactg gcactccatt gcatggaaga cgctgatcct
     1921 gactttatga aaaaggcaca agcaggtgat atcattgttg ccgggaaaaa ttttggatgc
     1981 ggttcgtcac gtgagcacgc tcccattgct attaaggcag caaagatttc atgtgtgata
     2041 gctcattcat ttgcgcggat attttatcgc aacagcatta acataggact tcccatcatt
     2101 gaatcggagg aagcttctgt cgatattgaa cagggcgatg aaattgctat agattttgac
     2161 tctggaataa ttaaaaatat tactaaaaat aaagagtata cggcaaaacc atttccacca
     2221 tttatgcagg acatcattgc caggggtggt ttgatggcta agattaaagc tgaaattgca
     2281 caatagatta ttgcacctgt tttatatttt tcccattcag gatatcggtg cctttagctt
     2341 taaatggtat aatgtaccat cttccttttt tatatttagc caattgtaat agcgtgggat
     2401 tgggaagatc ctgtataaat tgcgagctga attccagtgc aatatcaata tcactttcat
     2461 catggcgatg gtatttccca ttaagcttta tccgtattgc aggtgcgttg aatataaatg
     2521 agttgatggt aatagccggg ccattaagat ggaagttgcc atatattttc tgaaattcaa
     2581 ggttgctcag tttgtatttt aaatctgcaa gccatatgct tagtccattt tgcagacctg
     2641 tgtttgctaa ttttccatta tcaatggtaa attcaatctt tccaataatt ctgtccagca
     2701 acggctcttt tggtacgata gttacctgta attgtcctga tgcattccca aacaggcgat
     2761 tttcatattt ttcaacatat gaactgatat tttgcagccg tatagtattg aacgttgcta
     2821 ccagatttat ctgaggattt tcagggtgta gcgtaatgat acttcttcca gctatatcgc
     2881 caccaaagat ttttgcagta aacgaaggaa tggaaataat atttctggac atgatgtaac
     2941 tgcagtttat attgttaata gcaagcttgt cctttattaa tgtatgaatg gtaattttac
     3001 ccgagagttc caccggtatg gcaatttctt ttttagattt tcctgtgccc ggtatatttg
     3061 ttatatcagg ggcattcaga tttagttcct ggatctcagc attaattata aattttttaa
     3121 atgaggtatc caccgtagct atggaaagaa cacagggtat agagtagacc tgagcaggga
     3181 ttgcatcttt tttgcactga ttatttttta taattaacgt tactttatca tttattgaaa
     3241 gtattgtacc atttttgcct attgttacat ttgatactgt tacatctgca tcaatcatct
     3301 taccggtata actggcattg cctgtgatgt ctgcgttgat gctgctaata tcaatgttac
     3361 ccaataatgg agctatctcc ctgagtttaa tatcagcatc ggtaaccttg atggattgga
     3421 tgtctccatc aaaattaaaa atcaattgat taagaacaaa tgtagaattt tcaaccttcc
     3481 ctttaacatt tgctattgca acttttttct tatcgatgtc aacattacaa aatccattgg
     3541 ttatatagat catcgcattg ctggcaagct tacaacttcc ggttgctgtg ccctgaacat
     3601 ggtgtgttgt tattaataga tttgaaacgg taccgctgaa gattctgtat ggaaggatag
     3661 aagccaaatc atgtctgctc cacccataaa cccagagtaa atcacaggca tgtaatacaa
     3721 catctccgcg gatagaaaag ttgcccttct tagatatggc acagtcgccg gtaacttttc
     3781 ctctgttctg aggcattgtg atcgatagat tatgcaaatc gatagtgctt tcatttaatt
     3841 tgcaaagcgt gctgatacta tatctgcctt ccaggggttt taatacttct ggaggatttt
     3901 caagaataat ggctgtattt tctattgcaa ttgattctat ctgtgtttgt atgcttgatt
     3961 cttctttggc aagaagttcg tgtatcagtt ttgctaaatt tgatgtaaaa attttatttt
     4021 ctgtgtaata gataatatga attgctccat tgtttagata aatataattg attacaaact
     4081 gtttttgcaa caggggtagt aactgaaaac ggattgccga atcctttgca tgggcaataa
     4141 atggtttgtt gttgtcagta tcataaatat aaagatcagt aatctgtata ccgcgcaggc
     4201 tataatcaat ggtaccaata ctgacaggac gatttaatga tttttgcaat gtttcaacaa
     4261 tgatggattt taacaattct ctggggaaaa gatatacaaa tgctgccatc aaagcagcaa
     4321 taacaaacag tactatggaa agtactattg cactgtattt aactattttt gctggcttca
     4381 cgttttcgtt ctaatgctga ttttttcttg taaaaattta cggtcttttc aagctcttcc
     4441 agattagtac atataagctt gccttcatcc aatgttatat ttttatcttc cagaagttcc
     4501 accaccaggt agtccccctt gtcatgggaa atccctacca tattgaggag ttctttaacg
     4561 ccaaaatcaa atgtatagct ttcttttggt gtaatacgta tcttttgttt ttcaacctga
     4621 atcaataacg tgtcgtacat ccttccaaga ggatcgcgaa tcataagatt ttcaagttga
     4681 cggtatgctg tccatatacg ctcactcaac agttgaatca gtcgggtggc taattgtggc
     4741 tgtgtttgaa ccattgtttc aaaattttgc ctgttgattg ccataaggct ggtatcgcca
     4801 aatgttatag ctgaagcact tcgtggacgg ttttcaagaa gcgccatctc accaaaaata
     4861 tcgcctggtt ttaatacagc aaggagcacc tcttcatcca caatcttggt tatctttacc
     4921 ttcccaccct gaatgatata gagctcatcg ccgggttcat tttcgcaaaa tatcatctga
     4981 ttgtctttat acacacgtgt cagactattt ttctgaggat tttcaggcac cttgaatgga
     5041 gcattgatgg attttaaata agttatagcc ttgtccctgt ttatgccgtc agcacaatgt
     5101 tgtatataac gctgcagtgc atatgctgca tgaggtaaat ttttcttttt tagatagtat
     5161 tgaccaatgt tgaacagatg ttcaggatct tcttccactg cgttagcaaa agttaaccgt
     5221 gttatagcct gatcaaattc acgcagttta cgcgagaaga agcggataat cttcatggca
     5281 accgcaggat ttttctgtat gaggatgcca aattgatctc gctgtactga aatcagtgat
     5341 acattggtca tggcaacagc gccttcaata tgggcatgac cgctcataca gggaataaca
     5401 ccaaaaaaat caccaggtcc aagtagctga tttgattcct gagctactgg attttcctta
     5461 acaattttaa ctttcccctg ccgtattata taaaaattat cagcgttacg tttgccttca
     5521 acaacgataa aagaatttgc aaggtagttt acaactttaa attgttcctg caccatgtgt
     5581 tgccatcaat gtttgatttg aagtactgta atcatagcat agtatactat tgctaaaagt
     5641 aaacaaaaaa attattatca taaatccctt atggtaaaat cggaattatg gaaaataata
     5701 tcaagaattt ttaagggtta acaatgaaaa gaatctgcca tgtaattttt tatacattat
     5761 ccatacctat cattgattgt agcactcact tcaaccagta caaaggttat gctttaaggt
     5821 tggtgaggct tttaaccttt agctttatga tatgagagga taggttggtg tcagatggtt
     5881 catagtaggt agctttctga tatgatgcaa tcgcagcacg cggattgcgt agctgttcgt
     5941 atacctgacc acggtgcgag taaaattcct tctggtttat gagaactttt tgcatagaac
     6001 tgctggcggt tgcccccatt tttgggatgg ggagatcctt cttaaaacgt ttcatagggg
     6061 caaggacatc agtattgctt gttataatag aaggtttttg tttatgaaac tgtcctgcca
     6121 caggttttac actatttgat tgcacgtgtt ctatcatttc actgcacctc actataaaga
     6181 tacaaggaat gtatgggtaa agtcaataaa aaaatgatac attgtgatta tgatttacag
     6241 gcaatgaaac cacatgatgg tttacacaaa aaatgacatc aatagtaaca tcaatagtga
     6301 agacaggcaa tatgaagaat acacattagc catatgccta atcagatgta agatatttct
     6361 ctaaatattc attaaggtca gcaatatttt tacgtatcat ggatttaaat tcgggcggta
     6421 tgttctgttg catcactacc ctgtaataat tcagggcatc acgtacttta cgtttttgta
     6481 tcaattcatc ggctttttgc agcagcggtc tatatttgta aaatgcatat tccagtatat
     6541 ttttttctcg tgaaaggctg aattcatcgg gaagtttttt aaaatcatag gttacccgta
     6601 atattggcaa ttcctttttt ttgcgtcggt catgttgtaa aaatttctcg taaaatttac
     6661 ggtcttcttc cagtttgtgt aatgtttcag tatcctggat atcgtattca acatcatctg
     6721 ttttacgata atcacctaca atttcaatgt catcagcacg tttgcgtttg tcttcttcaa
     6781 gtatcttctt aaatatatct tcatcgctta tctcatctaa ctggctcttt ttttcatctt
     6841 ttataatatc acttataagt tcccattctt catcctcttt caacttttca gttttttcaa
     6901 caatctcgga ggattgtatt gtttcaccct gttttactgt atcattatat aatttcttta
     6961 aaaaatcttt tgcgctatca cctttcattc tgtctttatc aattggttta gaggcgggtg
     7021 cggcaatttt tggtagcttt tccaaaattt ttaagattgg ttccggatca agttcaattg
     7081 gcagcttccc ttcatatttc acggtggaaa cggcaggttg cttgcttccc tctaaaaggc
     7141 tggaatgcac tgtttttaaa ttttcaagtt ctgcccgtgt tttttccttt tcttcgtata
     7201 atttaaacat cctgtcggtg aggtcattga gttgattttg caacttttgt atttccgaag
     7261 tttttaaagc atcttctttg cttgatagct ccttcaagga actggcaacc tcttttttaa
     7321 gttcttcaag gtgtgatgat aattgtttga tctcattgct atagttgctt attgctgtat
     7381 cctcggtgtt ttgtaaccgc tgcttctcaa gaattcgttt agtaatttca tcaataatgt
     7441 tattaatatc aacatcctga ggtgcaaaac caatctgtat cttttcaggt agtctaatag
     7501 ggctatctaa tgaaattttt atttcattag gcgtggtttt tgctactgtt tcacgctgcc
     7561 gttgctgttg ctcttgtttg tgtcggtatt ttttcaggta attgatgttg gcatctattt
     7621 ttgagttggt ttctgcatca aggatgcgtt cgttgacacc ttcatagagc gagattgccg
     7681 tagggaagtc accctttttt gcatacacct ctgcattcat taaaattcta atataaatcc
     7741 tgaaacggga atagggtgga ataatctctt ctgcggtgta gggcagttta aaatcttcat
     7801 caatatcggt tctttcaggt ggtgtgtcgg ttgctcttcg tttgggtttt gtcccatcag
     7861 gatatgcttc ctctattgct aaatcgcttg cccgcctgcg atagattttt tcaggtgatg
     7921 attcactttt agcctgtggt gtgtcattgc ctgttattgc ccatatgagt ataacaatga
     7981 caatggcaag cgatgtaatc agtaatggta tcatgatact ataagttatt cctcactcta
     8041 ttgctgcagg cttactgtac agtaactatc gcccacaaca ataaaatata ataataatca
     8101 atgcaatgca acagactttt taaaaataat atgtgcgata gttaccggta aataacctca
     8161 tagtatatat cgtcaccgtt acgctgaaat caaaacaacc gctgacattt ttttgcatgg
     8221 atagc
//
LOCUS       DAOOJH010000106         8130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7562_length_8130_cov_11.686935, whole genome shotgun sequence.
ACCESSION   DAOOJH010000106 DAOOJH010000000
VERSION     DAOOJH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7562_length_8130_cov_11.686935"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLJ75_10800"
     CDS             complement(<1..183)
                     /locus_tag="PLJ75_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPD05740.1"
                     /translation="MAYGIVMFCVLGIIQGITEFLPVSSSGHLALLESIPYFNSIFSG
                     MDEGIKLFFNVALHLAS"
     gene            complement(185..1831)
                     /locus_tag="PLJ75_10805"
     CDS             complement(185..1831)
                     /locus_tag="PLJ75_10805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD05741.1"
                     /translation="MKEKKIRIIPIGGLHAIGGNMLVIEYDNEMLIIDCGISFPNGET
                     PGIDFMIPDFTYVLENKHKVKGIIITHGHEDHIGAIPFFLQKINVPVYATKLTLGLVA
                     SRLAERPVSQHTFIEIEPRDTIHIGPFIIEFLLVNHSIIDGVGLAIKTPLGIIIHTGD
                     FKIDYSPVDGKVADLHRFAEYGQQDVLLLISDSTNATKPGYTPSEIILKKKLLEIFAR
                     SKGRIFVASFASNIHRIQQVLEASQKYNRKVVISGTTMQKNVEIATELGYLQYKKDLI
                     IDAKDTGKYTYKKLVVLCTGSQGEPMSALHRMANGTHKHFRISAGDTVVITASVIPGN
                     ERMVYNVINSLMQLGALVYYEQDEDIHVSGHASQEELKLMITLTQPKYFLPVHGEYRH
                     LKAHAALAESLNIKPKDIIVAKNGDIIELTDKSFEKRGSIPLSTVYVDGNYIGDITDE
                     VIAERKTLSSDGVIVISIVIGDGLLLYPPAIIAKGFIGLKNDKFLQVLQKELIATIEK
                     HLAQNASSENLSVAVKKLVKMIAHRFTNNTPLVETIIQEI"
     gene            complement(1828..2262)
                     /gene="dut"
                     /locus_tag="PLJ75_10810"
     CDS             complement(1828..2262)
                     /gene="dut"
                     /locus_tag="PLJ75_10810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPD05742.1"
                     /translation="MITIKITLKHGAKKPDYVTHSASGADIYTFLDKPVILQPFQRVL
                     IPTGLSIELPPGYEAQIRPRSGLAINHGITLLNTPGTIDADYRGEIKLIVINLGEKPF
                     TIENNMRIAQMIIAQTVPATFTVVAELSDTKRNDGGFGHTGL"
     gene            2464..3528
                     /locus_tag="PLJ75_10815"
     CDS             2464..3528
                     /locus_tag="PLJ75_10815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPD05743.1"
                     /translation="MEDNGLTFFEETELTPETFAGLEKKVYDLRNLLEMGISLSSNLQ
                     FESLVESLLYSCIGQMFVEQVALLLQKDIDVNDYYIHMTKGYDTECKNGIVLLETSPL
                     IGFLANNPYPIEYETLLELPDIVEDLKAIAFLNPYIVVPLKSKDSLMGILLLGKKISG
                     EGFTNSEKEFLHYVARFGAVAVENSRLYLMSTLDRMTRLYIHHYFEIRLIEEMKRSQR
                     YNVPLSLLISDIDHFKRFNDTYGHVQGDSVLKEVAQLFLKDLRKMDIPSRYGGEEFAV
                     ILPQTDLEQAGIVAQRVRKLVEQHQFKGQEDALHVTISIGVAQYDPNKDKDTKGFIAR
                     TDKALYKAKQMGRNRVALFK"
     gene            3580..4395
                     /locus_tag="PLJ75_10820"
     CDS             3580..4395
                     /locus_tag="PLJ75_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05744.1"
                     /translation="MDLLEQFKEALKLEQEGKFHEALTLYTSIITVDNSFRAALLNLG
                     SLYYRMKRYSEALDSFMGALKLKEDYIVLFNIGTLFFRIGQYKKAIVALNQCKKLNSD
                     FVIALLVKGIAFSKLNHYKAALACFKEVLEKNPENKVALTAIVLLFYEQKNYSGALYY
                     LRKYEGYYKSFRFKDIAQDIILKCARHESAELIHFSLQKKEFKKFDEFVATVPETIFN
                     DSKGDIASKIKHLEKHIRTTPEAESLLSLSLCYLFQGNSQKAIYYLAQVTQSS"
     gene            complement(5031..5876)
                     /locus_tag="PLJ75_10825"
     CDS             complement(5031..5876)
                     /locus_tag="PLJ75_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPD05745.1"
                     /translation="MRNYPVYIVWAIVLMISLLSSCAPNSVMTKRNDTFTFGQNEENS
                     TANDSPVYSDMNPNVSATNTDEKSNYYQVGMASWYGREFQGRLTASGEPFDMNKYTAA
                     HRTLPFGSIIMVTNLENGKKIQVTVNDRGPFKKNRILDVSYKAARDLGMLEKGEAKVG
                     ISVVSYGNDSQPGNKSSDVGVFGEAVEDTNYQKNTYEQSSIGSGIAIQTGAFYSKLNA
                     DKLKEKIEGLVDKPVVVVKEGYFYKVRITNLDENQISKYRKILKKNDIPSYRISISDT
                     TDEAP"
     gene            6029..7090
                     /locus_tag="PLJ75_10830"
     CDS             6029..7090
                     /locus_tag="PLJ75_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HPD05746.1"
                     /translation="MIKSLKKYVMKIVASKPTITRKELEGVLDCLIQDNLASGSVVKQ
                     FEQALSTTIGLKYALATNSIYGAYHCIFSALGITAGDEVIIPSYFDAAPLKACHVVGA
                     IPVLVDIAENCYHPSIENIQGAITQKTKAIVIGHTFGFPAQLDLNDSAIPCILDISHA
                     IGMDAGELFDTAPSFAVASFAPSMMITTEMGGMIFTNNSRYFSYIRDIRNGMPREHIA
                     GFEYCMSDVHAAMGINQLLKLKDFVKRRRYIARIYYDALSKTHHSTFYAFSEHFTYQV
                     FPVLFDAQPDIVDKYFKKNAIEIYHPIDVPLHQYMKLRNLDFPHSDRLSKKLFSLPMY
                     PALTKREIEKVARIVTMLA"
     gene            7344..7748
                     /gene="queD"
                     /locus_tag="PLJ75_10835"
     CDS             7344..7748
                     /gene="queD"
                     /locus_tag="PLJ75_10835"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPD05747.1"
                     /translation="MYILTVEDYISAAHQLIGYKGRCENIHGHNWKIEISVCGEKLND
                     IGIMIDFHDLKAMIKEVLDEFDHKNLNDVDAFKSLNPSSEHLSRIIYEKMSIKLGQYS
                     KDNNTEIKLLSIVVWESATCRSAYQKNFLIST"
     gene            7828..7980
                     /locus_tag="PLJ75_10840"
     CDS             7828..7980
                     /locus_tag="PLJ75_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05748.1"
                     /translation="MKKIASLILIAVFAVAVAFVGCKKEEAPAPEEQVQEQPAEQAPA
                     EAPAQK"
BASE COUNT         2520 a         1382 c         1589 g         2639 t
ORIGIN      
        1 acttgctaaa tggagtgcaa cattaaaaaa aagttttatt ccttcatcca tgccgctgaa
       61 gatggaatta aaatatggga tggattcaag aagtgccaga tgaccagaag atgataccgg
      121 taaaaattca gtaataccct ggatgatacc caggacgcaa aacatcacaa taccatatgc
      181 catgttagat ctcctgaatg atagtttcta ccaatggggt gttgttggta aatcggtggg
      241 caatcatttt aacaagcttt tttaccgcta ccgacagatt ttctgaagaa gcgttttgtg
      301 ccagatgttt ttctatggtg gcaattaatt ctttctggag tacctgtaaa aatttatcat
      361 tttttaatcc tatgaaaccc ttggcaataa tagcaggtgg ataaagtagc aagccgtcgc
      421 caatgacaat acttataaca ataactccat ccgaggataa tgttttgcgt tcagcaataa
      481 cctcatcggt gatgtcacca atatagttgc catcgacata gacggttgaa agtgggatgc
      541 ttcccctttt ttcaaaactt ttatcggtaa gctcaatgat atcaccattt tttgccacaa
      601 taatatcttt aggttttatg ttgagtgatt cggcaagcgc agcatgtgct tttaagtggc
      661 gatattctcc atgaacaggt agaaagtatt tgggttgagt aagggtaatc attaatttta
      721 attcttcctg agatgcatga ccggatacat gaatatcctc atcctgttca taatatacta
      781 aagcgccgag ctgcatcaat gaattgataa cattataaac catacgctca ttgcccggta
      841 taaccgatgc tgttatcacc actgtatcac cggcactgat tctaaaatgc ttgtgtgtac
      901 cgtttgccat gcggtgaaga gctgacattg gctcgccctg cgaacctgta cacagcacca
      961 ccaatttttt ataggtgtat ttgccggtat cctttgcatc aatgataaga tcttttttat
     1021 actgtagata tcctaactcg gttgctatct ccacattttt ctgcatggta gtacccgata
     1081 taacaacctt acgattgtat ttctgggatg cttcaagcac ctgctggata cggtgaatgt
     1141 tagaagcaaa acttgctaca aaaatacgtc cctttgatct ggcaaatatt tccagaagct
     1201 tcttttttaa tataatttcg gatggtgtgt accctggctt tgttgcattg gtactatcgc
     1261 ttatgaggag cagcacatcc tgctgcccgt actcggcaaa acgatgcaga tcggcaacct
     1321 ttccatcaac cggagaataa tctattttaa aatcgcctgt atggatgatt ataccaagcg
     1381 gtgtttttat ggcaagcccc acaccatcga tgatagaatg attaaccagt aaaaattcaa
     1441 tgataaatgg accaatatgg atggtatctc tgggttcaat ctcgataaat gtatgctggc
     1501 ttaccggccg ttcggcaagc ctgcttgcaa caagtcccag ggtaagcttt gtagcatata
     1561 ccggaacgtt aattttttgg agaaagaacg ggatagcacc aatatggtct tcatgaccat
     1621 gagtaatgat tataccctta actttatgct tgttttccag aacataggta aaatcaggga
     1681 tcataaaatc tatccctggt gtttcaccat tggggaatga tataccacag tcaatgatga
     1741 gcatctcatt gtcgtactca ataacaagca tattgccgcc aatggcatga agtccgccta
     1801 taggaataat tcgtatcttc ttttctttca taagcctgtg tgtccaaagc ctccgtcatt
     1861 tcgttttgta tctgaaagtt cagctactac tgtaaaggta gctggtactg tttgagcaat
     1921 gatcatctgt gcaatgcgca tattgttctc tatggtaaat ggtttctcac ctagattaat
     1981 aacaattaat tttatttcac cacgataatc tgcatcaata gtccccggtg tgttgagtaa
     2041 ggtaatgcca tgattaatag caagtccact tcgcggtcgt atctgtgctt catagcctgg
     2101 tggcaattca atggataatc cggttggtat caatacccgc tggaagggct ggagaatgac
     2161 cggtttatcc agaaaagtat atatatcagc acctgatgca ctgtgtgtta cgtaatcagg
     2221 tttttttgca ccatgtttta gcgtaatctt tattgttatc ataataatta tattgaatta
     2281 tgaataaatt atatcatgcc atgatataat tgttgacatt ttttttaatt agtatataat
     2341 ttttttagaa attaatctat tataatttgt tttaacaaaa caatgcatgg gacatactat
     2401 gcataaatag agtcataacg tattggtacc gtaaaaaaaa caagaaaaat ttaagggata
     2461 gtcatggaag ataatggctt aacttttttt gaagaaacag aattaacgcc tgaaacgttt
     2521 gcaggacttg aaaaaaaggt atatgacctt cgcaaccttc tggaaatggg tataagcctt
     2581 tcttccaatc tgcaatttga aagcctggtt gaatcgttat tatacagttg tattggtcag
     2641 atgtttgtag agcaggttgc acttcttttg cagaaggata ttgatgtaaa cgattattac
     2701 atacacatga ccaaagggta tgatactgaa tgtaagaatg gtatcgtgtt acttgaaacc
     2761 agcccattaa ttggatttct tgctaataac ccatatccta tagaatacga aacgctattg
     2821 gaattgccgg atattgttga ggatttaaaa gcaatagcat tcttaaatcc ttatattgta
     2881 gtaccattga aatcaaaaga ttcattgatg gggattttgc ttttagggaa aaagatatca
     2941 ggggaagggt ttaccaattc agaaaaagaa tttttacatt atgttgcccg ctttggcgca
     3001 gttgctgttg aaaattcacg tctgtatttg atgtcaacac ttgaccgtat gacacggcta
     3061 tatatccatc attattttga aatccgcctc attgaagaga tgaagcgcag tcagcgatat
     3121 aatgttccgc tatcgctttt aattagtgat attgatcatt ttaaacgatt caatgatacc
     3181 tatgggcatg tgcagggtga tagcgtttta aaagaggtgg ctcaactttt tttaaaagat
     3241 ttacgtaaga tggatatccc ttcacggtat ggtggggaag aatttgcagt aattttgccg
     3301 cagacagatc ttgagcaggc aggaattgtt gctcaaaggg tgcgcaaatt ggttgagcaa
     3361 catcaattta aagggcagga agatgctctg catgtaacta tcagcatagg tgttgcacag
     3421 tatgacccca ataaggataa ggacaccaag gggttcatag cacgtactga taaggcttta
     3481 tataaggcaa aacagatggg aagaaataga gttgccctct ttaagtaaaa tgtcgatagt
     3541 aaatggtata gaatacatca taccatgaaa ggataaacaa tggatttact tgaacagttt
     3601 aaagaagcgc tgaaactgga gcaagagggt aaattccatg aagcattaac gttatataca
     3661 tctattatta ccgtggataa ttccttcaga gcagctttgt taaatttagg gtcattgtat
     3721 tatcgcatga aacgttatag tgaagctctg gatagcttta tgggagcact taagcttaaa
     3781 gaggattata ttgtgctatt caatataggt accctttttt tcaggattgg acagtataaa
     3841 aaagcaatag tggctttgaa tcaatgtaaa aaattgaact ctgattttgt aatagctttg
     3901 cttgtaaaag gtatagcttt tagtaagtta aatcactata aagcagctct tgcatgtttc
     3961 aaggaagtac tggaaaaaaa tcctgaaaat aaagttgcgt tgacagccat tgtccttctt
     4021 ttctatgaac aaaaaaatta tagtggcgca ttatattatt tgcgtaaata tgaaggttat
     4081 tacaaatcat ttcgcttcaa agatatagca caggatataa ttttgaaatg tgccagacat
     4141 gagagcgctg aacttataca tttttcatta cagaagaaag aatttaaaaa atttgatgag
     4201 tttgtagcga cagttcctga aacaattttt aatgatagta agggcgatat tgcgtcaaag
     4261 attaagcacc tggaaaagca cattagaaca acacctgaag cagaatcgct gctatcatta
     4321 agcctgtgtt acctgtttca ggggaacagt caaaaggcga tttattattt agcacaggtt
     4381 acacaatcga gttgatagtg tagtcacaat aaaaaaaatg taaccccacc aagaattatt
     4441 atggcaatga caattttttt tgcaaccata ggataatttt gcttaatgtt actccagact
     4501 agtgcgcata ctactctaca ggagtggcat agataataat atttttcaat gaaaagtaag
     4561 ggatgtggat taaatatcca atattaaata tagatgtatg atcgcaagct aattaccatt
     4621 tattgtgtag gcaataacac ttcagagggc ggtcccatat ggtattgact gatgcctctg
     4681 cttccagttc ttttcgatac ttcgtttgcg gtaaggaata caggtaaatg cctgcatacg
     4741 gcattttgct tctttgcaga atcgttaatg ttaataaaga gaaagataaa ggtgttcagg
     4801 ggtggctcgt agtgctcagc ataggagttt aagcaacgtt caatctttga tggtgacagg
     4861 tagtttatcc aatgttcata gattcctttt cccggagtta tttatatatt accaataact
     4921 atcgaatata atcgtatcta aaaaaatacg gtaaacgaat tattaatggt actattaatc
     4981 atttataaat attaggaata tagtacactg ataataaaaa aggcgctttt ttaaggcgcc
     5041 tcatcggtgg tatcacttat acttatcctg tatgatggga tatcgttctt ttttaaaatt
     5101 tttctatatt tactaatttg attttcatca aggttggtta ttcgcacttt ataaaagtaa
     5161 ccttctttta caactacaac tggtttatca acaagccctt ctatcttttc ttttaattta
     5221 tcagcattga gctttgaata aaatgccccg gtttgtattg caatgccact tccaatgctt
     5281 gattgttcat aggtgttttt ctgatagtta gtatcctcaa cagcttctcc aaatacacca
     5341 acatcactgg atttgttacc cggctgcgaa tcattgccat atgatactac ggatatcccc
     5401 acttttgctt cacctttttc aagcataccc agatctctgg cagctttata tgatacatca
     5461 agtatcctgt tttttttgaa tggccccctg tcgttgacag taacctgtat ttttttccca
     5521 ttctctaaat ttgttaccat aatgatgctg ccaaagggaa gcgtgcggtg agcagcagta
     5581 tatttgttca tgtcaaatgg ttcgccagat gcagtcaatc ttccttgaaa ttctcttcca
     5641 taccagcttg ccatgccaac ctgatagtag tttgattttt catcggtgtt tgtggctgaa
     5701 acatttggat tcatgtctga ataaacaggc gaatcatttg cagtagaatt ttcttcattc
     5761 tgcccaaagg taaatgtatc gttgcgtttg gtcataacgc tgttgggcgc acatgatgac
     5821 aaaagactga tcatgagaac tatcgcccat actatatata ctggataatt acgcataaat
     5881 ttcctccttg taaaagccat gaaattatac agacagtcat ccggggtatc tgcatagtaa
     5941 ttatcggaat tatgtaaata ctgctttatt taattttcaa aataaaaaat gatgcttgac
     6001 caaatagagg taatccgcaa ggttaccttt gattaaaagt ttaaaaaaat atgttatgaa
     6061 gatagttgca agtaaaccaa ccattacacg aaaagaatta gaaggagtgc tggattgtct
     6121 tatccaggat aatttagctt cgggttctgt tgttaagcag tttgagcagg cattgagcac
     6181 taccattggc ttaaagtatg ctcttgctac caactcaatt tatggagctt accattgtat
     6241 attttctgct cttggtatta cagccggtga tgaggttatt attccatcat attttgatgc
     6301 tgcaccgctc aaggcatgcc atgttgttgg cgctatccct gttctggttg atattgccga
     6361 aaactgttat catccatcaa tagaaaatat acagggagct atcacacaaa aaacaaaagc
     6421 gatagttatt gggcatacat ttggctttcc agcgcagtta gaccttaatg atagtgcaat
     6481 tccatgcatt cttgatattt cccatgctat tggtatggat gctggcgagc tttttgatac
     6541 agcgccatca tttgcagttg catcgtttgc tccatcaatg atgattacca cggaaatggg
     6601 tggtatgata tttaccaata attcgcgcta tttttcatat attcgcgata tacggaatgg
     6661 aatgccccgt gaacatattg ctggatttga atattgcatg agtgatgtgc acgctgcaat
     6721 gggtataaat cagttgctta aattaaagga ttttgttaaa cgaaggcgtt atatagctcg
     6781 catatactac gatgcattga gtaaaaccca tcacagtacc ttttatgctt ttagtgagca
     6841 ctttacctat caggtatttc ctgttttgtt tgatgctcag ccagatattg ttgataagta
     6901 ctttaaaaaa aatgcaatag agatttatca tccaattgat gtgccactgc atcagtatat
     6961 gaaactgcgt aatcttgatt ttcctcatag tgatagattg tccaaaaagc tgttttcatt
     7021 gccaatgtat cctgctctga caaaacgaga aatagaaaag gttgcaagga tagtaaccat
     7081 gcttgcctga aagattcagg taattattca tggggcagat tgttgtcaat agaagaattg
     7141 aaatcatggt gatagtatac gtattgttga actggttata gtttttgtat ggttcagtaa
     7201 aggtgatagt agtttattat tgtggcattg tggtatcgga aggttgtgta ttgctccata
     7261 ttgttaaaac aagtttacaa ttatattgat ttgattataa tattattcca tgcattatta
     7321 tattgttgcg aggtgaaaat accatgtata tactaacagt tgaagattat atctcagccg
     7381 cccaccagct tatagggtat aaaggcagat gtgaaaatat tcatgggcat aactggaaga
     7441 ttgaaatatc ggtgtgtggt gaaaagctca atgacatagg aataatgatt gatttccatg
     7501 atcttaaagc catgattaaa gaagttctgg atgaatttga tcataaaaat ttaaatgacg
     7561 tcgatgcatt taaaagttta aatcccagct cggaacattt gtcaaggatt atctatgaaa
     7621 agatgagtat caaactgggg caatattcaa aagataacaa tacagaaata aaattattga
     7681 gtatagtggt atgggaatcg gcaacatgcc gttctgcata tcaaaaaaat tttttgatta
     7741 gtacttgatt ttttttgata gttgtgattt gtggttgtgt aataaacaat aaaataaatt
     7801 ttattattat aaaggagtaa aagtattatg aagaagattg catctcttat tcttatcgct
     7861 gtatttgcag ttgctgtagc atttgttggc tgcaaaaaag aagaagcacc agctccagaa
     7921 gagcaggttc aggaacagcc agcagagcag gctccagctg aagctccagc acagaagtaa
     7981 ttaaacttca attttattgg caagagggac atcaatgatg tccctctttt gttaatagaa
     8041 agataatctg tattaattct tcatttattt tattatggca acctctaaaa atttcctttt
     8101 acatacagat tgccgcctga ttttaaagaa
//
LOCUS       DAOOJH010000107         7774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_7944_length_7774_cov_9.292266, whole genome shotgun sequence.
ACCESSION   DAOOJH010000107 DAOOJH010000000
VERSION     DAOOJH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7944_length_7774_cov_9.292266"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLJ75_10845"
     CDS             complement(<1..522)
                     /locus_tag="PLJ75_10845"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPD05749.1"
                     /translation="MFPEDVTVYLKNSVYVHITFFQWKLFIVGDSYSIKLLFFDTMLH
                     CDDYSTILNTAKKGSTPPLECAKLFFDAYMNKRITALPPCDLSLYTRKEQMVLRTLSQ
                     IPPGQTRSYGELALCCGMKNASRFIGNVMAKNMFPIIYPCHRIIKKNGTIGNYTGGVA
                     IKQFLLHHERDMDN"
     gene            complement(530..>1189)
                     /gene="ribF"
                     /locus_tag="PLJ75_10850"
     CDS             complement(530..>1189)
                     /gene="ribF"
                     /locus_tag="PLJ75_10850"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HPD05750.1"
                     /translation="PFTHELANLTAEEFLYRIIFYHIGKANIVVGYDHAFGRGREGNY
                     EYLQAIAHKEGIMVKRVEPVCIQSKPVSSSWIRAEIEDGNIAMANELLGRAYSLSGTV
                     VQGQMRGRLLGYPTANIAPDDMDKIIPKDGVYAVRVTINNTMYDGMLNIGNNPTFANT
                     ERTIEVNIFDFNSDIYGMQVEVYFYDRIRDDKRFESVTALKEQLHNDMIAAQTMLKKR
                     T"
     assembly_gap    1190..1289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1296..2369)
                     /gene="aroC"
                     /locus_tag="PLJ75_10855"
     CDS             complement(1296..2369)
                     /gene="aroC"
                     /locus_tag="PLJ75_10855"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPD05751.1"
                     /translation="MFAPNTFGQVLRITTFGESHGIAVGVVIDGLKPDIPIDENDIQK
                     QLDKRKPGQSTISTQRKEADHVAIVSGIFDGKTTGTPVCMIVYNKDKDSMAYESIKDI
                     FRPGHADFTFWKKYGIRDYRGGGRSSGRETVGRVAAGAIALKQLKANGVQVLAYAEEI
                     AGIQGTVEDYSFVEKNPVRAADPQKAPLMEEAIKKAAAEHDSVGGIVKCVVKGLPAGL
                     GDPVFFKLDGRLAWAIMSIGAVKGVEFGSGFAAARKRGSQNNDAMKDGKFLTNNAGGI
                     LGGIATGQDIVMRIAVKPTPSIAMLQQTMNIYSHNVDITIKGRHDPCIVPRIIPVVEA
                     MVALVCYDAWLVQEKIGWHDSTI"
     assembly_gap    2399..2498
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3110..3859
                     /locus_tag="PLJ75_10860"
     CDS             3110..3859
                     /locus_tag="PLJ75_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HPD05752.1"
                     /translation="MAPRNKGIQLMINKIEKAPDIPSLVVSQIIELISSGVLKPGDRL
                     PSELEMTRRFGISRISLREAMKLLEAKGYIESQGRKGKYVKSVINDAIRTPIEGLIQI
                     DHKKIWELLEVRRIIDAEAAALAALRANEEQRRKLNSFKTQIELMGVENLIQKREGGK
                     FYAQFYNHLYEATNNTIFAHVLNAISTILRGVLPYSREKLSKVKNTSDDIYNQHLKIL
                     NAINSNKPDVAREAVVEHIDYLEKTLKKILK"
     gene            complement(3871..6045)
                     /locus_tag="PLJ75_10865"
     CDS             complement(3871..6045)
                     /locus_tag="PLJ75_10865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD05753.1"
                     /translation="MLLTSTINAKGVYYTMLNRQIRELAMKGEKAITVENVLGQRYIG
                     GGLHHNAVITIHGTPGQDLGAFMNGPRIVVHGNAQDGVGNTMNNGVIVIHGKAGEIPG
                     HSMRGGIIIIRDDAEYRAGIHMKEYEDQIPLIIIGGTVKDYCGEYMAGGRIVVLNRLN
                     DNYPAGNDVGTGIHGGVIYIRGNVDSRRLGVGAVFNTLDADDKNFLQQAISLFKQEFP
                     YIELSNISIDDFLKITKKGHRPFANLYSPAMNIKTKKPIHLNLTPPCTYACPTGIPTP
                     VFLNLIKDGKVKDAQMLMDEYTPFRMSVCGTVCPAPCMEACSRNMIDGPMDIPYLARE
                     YYPDFNPVVYGKPNNKKVAIIGAGPAGLSAAWQLARRGYTVTVFEKTGDIGGKVRLAI
                     PKDRLPDEILGRDLERIKSLPIEFKVNTHVDAKLFSRIYQDNDLVLIATGSYISRKLL
                     VKGGERIVSGLEFLMGINEGNPMDLTDNDVVIIGAGNVGMDIACESWRHGAKTVTAVD
                     IQKPLSFGKEREMAERLGTAILWPRSIDYIDDTMIYFTNGESMHADVIFISIGEKPDI
                     SFLPETVMVNERGYIATADKSFKTSDSKVYACGDIVTTGLITDAIGMGRLAAMEIHAY
                     CEGEDFIYPGKTPVSRRRINTAYFGGETHEIDRCISCGTCIFCDKCVQICPQGAITRN
                     GEIFTIDSELCTACYTCVNVCPRGALQPGDFDAIANDVLFKK"
     gene            complement(6063..7580)
                     /locus_tag="PLJ75_10870"
     CDS             complement(6063..7580)
                     /locus_tag="PLJ75_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPD05754.1"
                     /translation="MKNNWKIIPPFHPEIDYARCDYKNGCTICAHECSWGEISLKPEK
                     LDNGTMVYKPGANLTSCGSCQRCVKMCPYSAIHIVNSPMYTSHGYWTPDYVGNIVKQA
                     SSEGGVLMVGNGATGPQRRYFDHIMFDACQVTNPSIDPLREPMEIRTYLGRRPSKWGG
                     EQYPLLKLEVPIMFGAMSFGALNLRVHEAEAKATSEVGTYWNTGEGGFHISLRERYGA
                     NTIVQVASGRFGVSEDYLKSAAAVEIKIGQGAKPGIGGHLPGEKVSREISETRMVPVG
                     SDALSPAPHHDIYSIEDLRQLIYAIKETTEYKVPISVKIAAVHNVAAIASGIARAGAD
                     IIAIDGFRGGTGATPLQIRQNSGIPIELAIAAADERLRQEGIRNQVSLVASGGIQCSS
                     DVLKAICLGADAVYVATPVLIALGCGMCQRCFTGKCAYGITTNKPGLAERIDVEEAAW
                     ALKNLLHAWAEEIEELMGSMGINAIEAMRGNRDRLRGVGLNQEELRILGIKHAGA"
     gene            complement(7586..>7774)
                     /locus_tag="PLJ75_10875"
     CDS             complement(7586..>7774)
                     /locus_tag="PLJ75_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05755.1"
                     /translation="GGAIVLNDRNKLRPVVCARVGSTYYASSEECSIRLLSPKVDALW
                     SPRGGEAVIVDLAKEEVQ"
BASE COUNT         2157 a         1849 c         1328 g         2240 t
ORIGIN      
        1 gttatccatg tcacgttcat ggtgcaataa aaactgcttt attgcaacac cgccagtata
       61 atttccaatg gtaccatttt ttttaataat tctgtggcag gggtatataa tgggaaacat
      121 attttttgcc ataacattcc caataaatcg cgaggcattt ttcatgccgc agcaaagtgc
      181 cagctcgcca tagcttctgg tttgtccagg tggtatctgt gatagtgtgc gcagaaccat
      241 ttgttccttc cgggtataca gtgataaatc acagggaggc agtgctgtaa tacgcttgtt
      301 catgtatgca tcaaaaaata acttcgcaca ctctaacggc ggagtgcttc ctttttttgc
      361 agtattaaga attgtactat aatcatcaca gtgtaacatg gtatcaaaga atagtaattt
      421 tatagagtaa ctatcgccaa caataaaaag cttccattga aaaaatgtaa tgtgcacata
      481 cacactattt tttaaatata ctgttacatc ttctggaaac actatacatc tatgtccttt
      541 tttttaacat tgtttgcgct gcaatcatat cgttatgtaa ttgttctttt aatgcagtca
      601 cagattcaaa ccgcttatca tcgcgaatgc ggtcataaaa atacacctcc acctgcatcc
      661 cgtagatatc actgttaaaa tcaaagatat tgacctcaat ggtgcgctcg gtattggcaa
      721 atgttggatt gtttcctata ttgagcattc catcatacat ggtgttgttg atagttactc
      781 tcactgcata tacgccgtct ttaggaataa tcttatccat atcatcaggt gctatattag
      841 cggtgggata gcccagcagc ctgccacgca tctgtccctg caccaccgta ccactgaggg
      901 aatatgctct tcccagcaat tcatttgcca tagctatatt cccgtcttca atctcggcac
      961 gtatccacga cgatgaaaca ggctttgact ggatacatac cggctctact cgttttacca
     1021 taatcccttc tttatgagct atcgcctgca gatattcata gttgccctct cttccccgac
     1081 caaaagcatg gtcataccca acaacaatat tggctttccc aatatgataa aaaataatcc
     1141 tgtataaaaa ttcttcggct gtaagatttg caagttcatg ggtaaatggn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctaaactata tagtactgtc atgccaccct
     1321 atcttctcct gcaccaacca tgcatcatag cataccagcg caaccattgc ttcaacaacc
     1381 ggaataatgc gtggcacaat gcagggatca tgtctgcctt taatagtaat atctacatta
     1441 tggctataga tattcatggt ttgctgaagc atggcaatgg aaggggttgg ctttaccgca
     1501 atccgcatta caatatcctg ccctgtagca atgccgccca gaatgccacc ggcattattg
     1561 gttaaaaatt ttccatcttt catggcatca ttattctggc taccccgttt gcgtgctgcg
     1621 gcaaagccac taccaaactc aacacctttt acagcaccaa tagacatgat agcccaagca
     1681 agccgtccat caagtttaaa gaacaccggg tcccccagtc ctgcaggtaa gccctttaca
     1741 acacatttta caatgccgcc aacggaatca tgttctgctg ctgctttttt tatagcctct
     1801 tccatcaatg gcgctttctg cgggtctgct gcccgtaccg gatttttttc cacaaatgaa
     1861 taatcctcaa ctgttccctg tatccccgct atttcttctg catacgccag cacctgtaca
     1921 ccgtttgcct ttaattgttt cagggcgata gctccggcag caaccctccc caccgtttca
     1981 cgtcctgaag agcgtcctcc acccctgtaa tcccgtatac catatttttt ccagaatgta
     2041 aaatcagcat gccctggcct gaatatatct ttgattgatt catacgccat ggaatcctta
     2101 tctttattat atactatcat acatacagga gtgcccgttg ttttcccatc aaatatccct
     2161 gatacaattg caacgtggtc agcttctttt cgttgagtgc ttatggtgct ctgcccgggc
     2221 ttccgcttat caagctgttt ttgtatatcg ttttcatcaa tagggatgtc aggttttaag
     2281 ccatcaataa ccacacccac tgcaatgcca tgactttcgc caaatgtggt tattcgcaat
     2341 acctgcccaa aggtattggg agcaaacatc cattgcccca taacaaatga aatctgctnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc tatcatcctg caggttttat
     2521 gtgttccatg cttatcagca aacatggatt caatcatttc atcggtttcg taaaacggga
     2581 cacctatctc ctcagctatt cgtttcccca cggttgattt tccgcaaccc ttcggtccac
     2641 ttatgataat cttcatgtac gtttttccca aaaatagatt attgcaggca atggtaacaa
     2701 cccggaatat aattataaca ataaatgcaa ttaacaagca tcagtcaata aaaagttact
     2761 atcaccccaa aaactattac attacattca tactatttgc caatactatg ttggtcaacc
     2821 aatactctac cgtctacaat gttgcctatt gtattacaat aaacaaatca acaaaatttt
     2881 tagatatttt gtatagtaat gatataataa tacatatttt ttgctttaat taaaattatt
     2941 ttattttgta tagatagtca tacaactttg ctatattaat tgtgttgaca caatatcatt
     3001 aatattataa tatgtaatac aataatactt tggagtaatg atcattctga tatatcattt
     3061 catggcacta tgaaaaacac tacactacca tataaaggag gccacatcaa tggcaccacg
     3121 taacaaaggc attcagctca tgatcaacaa aattgaaaaa gcccctgaca tcccgagtct
     3181 ggtagtttca cagattattg aacttatttc atccggcgtg ttaaagccag gtgacaggct
     3241 gccttcagaa ttagagatga ccagaagatt tggtattagc cgtatatcct tgcgcgaggc
     3301 aatgaaatta cttgaagcaa aaggctatat cgaatcacag ggcagaaaag ggaaatatgt
     3361 aaaatcagtc atcaacgatg caattagaac tcctattgaa ggattgatcc agattgacca
     3421 taaaaaaata tgggaacttt tagaggtacg gcgcattata gatgccgagg cagccgcttt
     3481 agccgcatta cgcgccaacg aagagcaacg gcgtaagctc aattcattca aaacccagat
     3541 tgaacttatg ggtgttgaaa atttgattca aaaacgtgaa ggtggtaaat tttatgcaca
     3601 attttataat cacctttatg aagccacaaa caacactata tttgctcatg ttttaaatgc
     3661 tatttcaacc attttgcgtg gtgtacttcc ctatagccgt gaaaagctat caaaggtaaa
     3721 gaacacatca gatgacatct ataatcagca cctcaaaatt cttaatgcaa tcaattccaa
     3781 taaacctgat gtagccaggg aagcagttgt agagcacatt gattatttag aaaagacatt
     3841 aaagaagatt ttgaagtaag attattgtga ttatttctta aacaatacat cattggcaat
     3901 agcatcaaaa tcaccaggct gtaatgcccc ccgcgggcaa acgttaacac aggtataaca
     3961 tgctgtgcac aattctgaat caatggtaaa aatttcacca tttcgtgtta ttgctccctg
     4021 tggacatatc tgcacacatt tatcgcaaaa aatacaggtg ccacagctta tacatctatc
     4081 aatctcgtgt gtttcacctc caaaataggc agtgttaatt cttcgtcgtg acaccggtgt
     4141 ttttcctgga tagataaaat cttcgccttc acagtaggca tgtatttcca ttgcagccaa
     4201 cctgcccatc ccaatagcat ccgttattaa acctgtcgtt acaatatcgc cacaagcata
     4261 aaccttgcta tcagaggttt tgaatgattt atctgccgtt gcaatatatc cccgctcatt
     4321 taccattact gtttcaggta aaaatgagat atctggcttt tcaccaatgc ttataaaaat
     4381 aacatcagcg tgcatcgatt cgccattggt aaaatagatc atggtatcat caatataatc
     4441 tatgcttctg ggccagagta tagcagtacc aagcctttct gccatctcgc gttctttacc
     4501 aaaggataac ggcttttgaa tatcaacagc agtaactgtt tttgcaccgt gacgccatga
     4561 ctcacaggct atatccatcc caacattccc ggcaccaata atgacaacat cgttatcggt
     4621 taaatccatg gggttgcctt catttatgcc cattaaaaat tccagtcctg aaacaatgcg
     4681 ctcaccaccc ttaaccaaaa gtttacgtga aatgtaacta cctgttgcaa tgagcaccag
     4741 atcattatct tggtatattc tggaaaagag ctttgcatcc acatgagtat tgaccttaaa
     4801 ttctattggc aaacttttga tacgttccag atcccttccc aatatctcat ccggcaacct
     4861 gtcttttggg attgccaatc tcaccttgcc accaatatca cctgtctttt caaatactgt
     4921 cactgtatat cctcttctcg ccagttgcca tgctgcagaa agcccagcag ggcctgcgcc
     4981 aatgatagca acttttttat tgtttggttt tccatataca actggattaa aatcagggta
     5041 atattcacgt gccagatacg gaatatccat tggtccatca atcatgtttc ggctgcaggc
     5101 ttccatgcag ggcgcagggc aaaccgtacc gcatactgac atcctgaatg gcgtatactc
     5161 atccataagc atctgtgcat cttttacctt accatccttt ataagattta aaaataccgg
     5221 cgttggtatt ccggttggac atgcataggt gcatggcggg gtcaggttaa gatgaattgg
     5281 tttttttgtt ttgatgttca ttgccggtga atacaggttt gcaaatggac gatgtccttt
     5341 tttggttatt tttaaaaaat catcaatgct tatgtttgat agttctatat aggggaattc
     5401 ctgcttaaaa agtgatatcg cctgctgtaa aaaattttta tcgtccgcat ccaatgtatt
     5461 aaacactgca ccaactccca gcctgcggct gtcaacattt cctcgtatat agatgactcc
     5521 gccgtggatg ccagtaccca catcattccc tgctggatag ttatcgttca gcctgttcaa
     5581 tacaactatc ctgccacctg ccatatattc accacaataa tcttttacag ttccacctat
     5641 gatgatcagt ggaatctgat cctcgtactc tttcatatga atgccagctc tgtattcagc
     5701 gtcatcccgg atgatgataa tgccgccgcg catggaatgt cctggaatct cccctgcttt
     5761 tccatgaata actatcaccc cattattcat cgtatttcca acaccatcct gtgcattacc
     5821 atgtaccaca attcgtggtc cattcataaa agcacccaga tcctgtcccg gagtaccatg
     5881 tatggttata acagcattgt gatgtaatcc accgccaata tatcgctgcc ctaacacatt
     5941 ttctacagtg atagccttct cacctttcat tgcaagctcg cgtatctgcc ggtttaacat
     6001 tgtatagtat acaccttttg cattaatagt cgatgtcaaa agcatagtta cctcctgctc
     6061 cattacgctc cagcatgttt tataccaaga atgcgcaact cttcctggtt taaaccaaca
     6121 ccacgcagtc tatccctgtt accccgcatg gcctcaattg catttatacc cattgagccc
     6181 ataagctctt caatctcctc agcccatgca tggagaagat tttttaaagc ccatgccgct
     6241 tcttctacat caattcgttc agcaagacct ggtttatttg ttgttatgcc atacgcacat
     6301 tttccggtga agcaacgctg gcacattcca caacctaaag caataaggac aggagtagca
     6361 acatacactg catctgcgcc caggcatatt gctttcaaaa catcggaaga acactgtata
     6421 ccacccgaag caacaaggct tacctgattc cgtatccctt cctgccgcag gcgttcatca
     6481 gcagcggcta ttgccagttc aatcggaata ccggaattct gccgtatctg caacggtgtt
     6541 gctccagtac cacccctgaa gccatcaata gcaatgatat cagcaccagc acgtgcaatg
     6601 ccacttgcaa ttgcagcaac attatgtacc gcagcaatct taaccgaaat gggaacctta
     6661 tattcagtag tttcttttat agcgtaaata agctgtcgca aatcttcaat agaatatata
     6721 tcatggtgcg gtgcaggtga cagggcatca ctgcctacag gaaccatacg ggtttctgat
     6781 atttcccgtg aaaccttttc acccggcagg tgcccgccaa tgcccggttt tgcaccctgc
     6841 ccaatcttaa tctcaacggc agcagccgat tttaaataat cctcgctgac accaaaacga
     6901 cctgatgcaa cctgcacaat ggtattggcg ccatatcgct cacgtaatga gatatgaaat
     6961 cctccttcac cggtattcca gtaggtgcca acctcgcttg ttgcttttgc ttcagcttcg
     7021 tgaacacgca aattcagggc tccaaagctc atagcgccaa acataatggg aacctccagc
     7081 tttaaaagtg gatattgttc cccaccccac tttgacggcc gcctgccaag gtatgttcgt
     7141 atctccatag gttcgcgcaa tggatcaatt gatggattgg taacctgaca ggcatcaaac
     7201 atgatatgat caaaataacg acgctgcgga ccggttgcac cattaccaac catcagcaca
     7261 ccaccttctg aagaagcctg ctttacaata ttgccaacat aatccggagt ccagtatcca
     7321 tgacttgtat acatagggct attcacaata tggattgcgc tataaggaca catcttaaca
     7381 cagcgttgac acgaaccgca tgatgtaagg ttggcacctg gtttatatac catggtgcca
     7441 ttatccagtt tttcaggttt taacgaaatt tccccccatg aacattcatg cgcacagatg
     7501 gtgcaaccat ttttgtaatc gcatcgtgca taatcaattt caggatgaaa aggtggtatg
     7561 attttccagt tattcttcat actcatcact gcacctcctc tttcgctaag tcaacgataa
     7621 ccgcctcgcc accccttggc gaccatagcg catcaacttt aggcgatagt aacctgatac
     7681 tgcactcttc cgaagatgca tagtaggtag agccaaccct ggcgcataca accggcctga
     7741 gcttatttct atcattaaga actatcgccc cccc
//
LOCUS       DAOOJH010000108         7631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_8118_length_7631_cov_8.842793, whole genome shotgun sequence.
ACCESSION   DAOOJH010000108 DAOOJH010000000
VERSION     DAOOJH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8118_length_7631_cov_8.842793"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLJ75_10880"
     CDS             complement(<1..224)
                     /locus_tag="PLJ75_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05756.1"
                     /translation="MSFCAVEHFHTIPTFYKWGYRGVCHRNNKQPYRKVQRIWMQAHE
                     ATFSLVKTKTALPPFFITAGLKEFSEGTDAV"
     repeat_region   457..868
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=457..491
                     /rpt_unit_seq="gtctcaatcccctatcagcggggcagttttcagac"
     gene            1687..1959
                     /locus_tag="PLJ75_10885"
     CDS             1687..1959
                     /locus_tag="PLJ75_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPD05757.1"
                     /translation="MNGDDPINIALTDTIDLHVFHPADITAILDEFLNNAVLQGYLQV
                     EIIHGKGKSVVKHTVHNYLKNDKRVLQFFDKSANWGRTIVILKKEV"
     gene            2034..4454
                     /locus_tag="PLJ75_10890"
     CDS             2034..4454
                     /locus_tag="PLJ75_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPD05758.1"
                     /translation="MENMKSSNTTETQIISHDEAHMLEWDTLISFIANLCYTQPGKNY
                     CMGMQPLDYNEILEQYAKINCLKEILSIGHHPDFSGIHDITYLLEKSSKGATLQLDEI
                     FKIRLFITAQYRITSFFKSIQYPHNALTELQMLHPCEQLYTMLISSLTDSGNLNENTY
                     PVLKKLQREINDIRNAIEKSLQNLIHSHEYQQIVQEKIFTVKNNRYCIPIKTTFKNKL
                     KGTIADISSSGATLYIEPDSIHDLNNTLLYKELDLQREIEKILQILSSTIAEHAHLLM
                     ENAAIIGYLDFCTALARFAVTYNASTPALSRQPYLRLFNAKHPLLVTMHGKKIVPCDI
                     ECGINFKCLIISGVNTGGKTVLLKMLGLFALMVRYGLPITASPDSTAGYFESVYADIG
                     DEQNIMQSLSTFSGQIKNIVRILNLANEKSLVLIDEIIVGTDPKQGAAIAQAVLQYLV
                     NKKAVVAVTTHYSQLKEIASVDSQFENASVVFDIHTMQPTYQVLMGIPGASYTIEIAK
                     NLSLPQKIIDDAISLLDSTSLTVDALLSQITQQSHTLHQKEQELALLKQELNQLKNDY
                     DKKINELQQQIEKVTKEQAIEFVHELHQYRNTIIDKIRTMHSMSMKDATQLVNDITQM
                     QLSLQDTIKQLQKPSEDFLPCTVDTVQKGSKVFIKPLEQYGYVEDVDEKSKQLKVRMG
                     TVNSRFSFDDVYCIPESKKLTTVKTKTNTIATQDIQHIPMSIQTKYNTIDLRGLYVDE
                     ALQKMDHELDNLLKNNIPTAIIIHGHGTGSLKKAIRQYLKHSFYVKGYRPGQPEEGGD
                     GVTVVQLR"
     gene            complement(4563..6191)
                     /locus_tag="PLJ75_10895"
     CDS             complement(4563..6191)
                     /locus_tag="PLJ75_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HPD05759.1"
                     /translation="MSKPITKSWWKHGVLYQIYPRSFKDSNNDGIGDLEGIIQSLDYL
                     QDVGVDGIWLSPIYRSPMYDFGYDVSDYCSIDPIFGTIANFKKLLKEAEKRNIAIIMD
                     MVLNHTSHLHPWFVESRSSVDNPKRDWYIWHPGKNGKVPNNWMAAFGGRAWTWDELTR
                     HYYLHLFTEQQPDLNWHNPEVKKAMVDVLRYWLDMGVKGFRFDVINYIVKDINVRSNP
                     YRLRLTYPRRHDLQVHIYDRNQPEIHDILKELRQLLDSYGDTMSVGETYPNEGEKEPA
                     MAASYLGNNDELHLSFDFSTIYARFNAREFERILKNWYHAIGDSGWPCHVLSNHDQSR
                     AITRLANNDVQKAKLLAVMLLTQKGTPFIYYGEEIGMVDGKIKRSQIQDPLGKKYWPF
                     HKGRDKARTPMQWDMTQNAGFSNGKPWLPVNSDYVTTNVLQQQGNKQSILHLYKDCIA
                     IRRLHPALYAGDIEFVPAPDDVLLYKRCHNNEQLMIALNFSKAKKDVTIGLEGEVLLS
                     SCGNSSIHSSHSSHSSIHGGALTLDAYEAVIIKV"
     gene            complement(6184..7386)
                     /locus_tag="PLJ75_10900"
     CDS             complement(6184..7386)
                     /locus_tag="PLJ75_10900"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPD05760.1"
                     /translation="MNILDLRPENFKKAFPDSKVMHVNGKSIIKSAMKEGNHWVAMAV
                     NTRHEIAIPGIIKASMELNAPVIYELALSESDLSGGYTGLTPADFIKLVVSLNDKMGN
                     AGEKAVPFTCHRDHTTVQEVSRKAFDKADAIIKASIEAGYGSFSIDVSFLPMEQNIAL
                     SILLSEQVIRAGLMYESEVGEIGGAHGNSSVSEAVTLVEALTKLGQCPDLIAINNGTT
                     HGNVRGNIDLQLTHNTYRAMSPWGVGIAQHGTTGTSIEDVGQFYRYGIFKANVGTLWQ
                     NVIWGIDMDEYGVAVEKGPTYEKDPDKGISMELWKKMEEYALANNMKGGNMKKLFKPF
                     KDAMNDEYYNNAKIRDRVDKAVYETAAAFFKALKAVGKGDDVKAQMETITTPSKQGSK
                     ESKERDYE"
BASE COUNT         2352 a         1636 c         1334 g         2309 t
ORIGIN      
        1 acggcatccg tgccttcgct gaattccttt aatcccgcag tgatgaaaaa tgggggcaga
       61 gccgtttttg ttttcacaag agaaaaagtg gcttcatgcg cctgcatcca aatgcgctga
      121 accttcctat atggctgttt attgttgcga tgacacaccc cccggtatcc ccatttataa
      181 aaggtgggaa tggtgtgaaa atgttcaaca gcacaaaaac tcataaaatt tcgtaatttt
      241 aaaaaaggga aaattttttc tgatggtata gtgttcactt aacaataaat aagcacgaat
      301 ttctatctct ttttaaaatc taaaacattt ttacatccag tacacaatac agtatgccca
      361 taaaacaatt cctaacagct aatatctaca tcaccagcaa ccaccaaatc cacgctacaa
      421 ctttcaacat tataactttc aactttcaac tgttacgtct caatccccta tcagcggggc
      481 agttttcaga cgtggagcga tgagctctac ctttcagttg taaaaagttt tatggtctca
      541 atcccctatc agcggggcag ttttcagact gtactatcta tccttccaga agtcatactg
      601 gcagactacg tctcaatccc ctatcagcgg ggcagttttc agactatctt gcatacgggg
      661 atataagtca tggaattaat gtagtctcaa tcccctatca gcggggcagt tttcagacta
      721 gtaggtttta ttaataaagg caagccgggg tataatctta gtctcaatcc cctatcagcg
      781 gggcagtttt cagactcgat agagaactcg tgcggatggc gcagaagatc ctggtctcaa
      841 tcccctatca gcggggcagt tttcagaccc ttaccctgta aacctctgga atttccagcg
      901 tttttaagcc cattttcgcc aacctatcgc aaaaatcact attttttcca tgattttgta
      961 ttacaaactt gtaactgctg gattttccag cacttccacg tgcgccaacc tccccccaaa
     1021 aacgccactt tttacacttt tagcaggttg gcgatataca aagttgtata tcatggtact
     1081 gttttcaaag aatatcttac atcatctgtt actgcctata gcatgaagca tttcctgtca
     1141 actatttttt tacttctgtg aaaaatttta ccgtaactat cgcccataaa aattacaatg
     1201 gggtcagagc atgtataact tttgttgggc gacaaaagtt atatcctttt gtaaggggat
     1261 atttatgcgc taaatacccc cttaccaacc ctgtggagcc acctcaaacg gcggtgtgcc
     1321 ttatcccgct gtatggatta tatctttgcc atcgtgctgc ggaagacatc ctgtctttgt
     1381 cctcgcacga gccatatggc gctacggcat ccatgccttc gctgtttttt catttttact
     1441 cagtagggat cactttttgt aacagaacat tcactattca cttcaaaatc tcataggtgt
     1501 gacaaaaaat ggggtcagag cttaaaactt tttcaaaaca actatcgttg ccatgacaat
     1561 gtactttaca aaacatggcg tattgtgtat attgaaaaca gcttcgtaac gcattattga
     1621 ctgtatggag cattcctgat aacggatgat ggatttctgg aaccaatagc tgagtaacaa
     1681 ccgcttatga atggtgatga ccctattaat attgcattaa ccgatactat tgatctgcat
     1741 gtgtttcatc ctgcagacat tacagcaatt ttagatgaat ttttgaacaa tgctgtacta
     1801 caggggtatt tgcaggttga aataattcat ggaaaaggga aatcggttgt aaaacacact
     1861 gtccacaact acttaaaaaa tgataaacga gttttgcaat tttttgataa atccgctaat
     1921 tggggaagga ctatcgtaat acttaaaaaa gaggtttaat gcagttgcat taaaggtgct
     1981 ttgcaataat tactctttac aataatgttt tggtacaata ctattaggaa gttatggaaa
     2041 atatgaaaag cagtaatacc actgaaacgc agattatatc tcatgatgaa gcacacatgc
     2101 tggaatggga tacccttata tcatttattg ccaatttatg ttatactcag ccaggcaaaa
     2161 actattgcat ggggatgcaa ccacttgatt ataatgagat actagagcaa tatgctaaga
     2221 ttaattgtct taaagaaata ttgtccatag ggcatcatcc cgatttttcg ggcatacatg
     2281 atattacgta cttgctggaa aaatcatcaa aaggagcaac tttacagctt gatgagattt
     2341 ttaaaatacg attgtttatc actgcacaat atagaattac ttcttttttt aaatcaatcc
     2401 agtatccaca caatgcgcta accgaattgc agatgctcca tccctgtgaa cagctatata
     2461 ccatgctcat ttcttcatta acggattcag gaaatcttaa tgaaaatacc tatccggtac
     2521 ttaaaaaact gcaacgagag attaatgata tacgcaatgc aattgaaaag tcattgcaaa
     2581 atcttattca ttcgcatgag taccagcaga ttgttcagga aaaaattttc acggtaaaaa
     2641 acaatcgata ctgtataccc attaaaacaa catttaaaaa caaattaaaa ggaactatcg
     2701 cagatatatc atcttcaggt gcaacactgt atattgaacc tgacagcatt catgacttga
     2761 ataacaccct gttatataag gaattagatc ttcaacgcga aatagaaaaa atattgcaaa
     2821 tactatcgtc cacaattgct gaacatgctc atttgctaat ggaaaatgca gccataatag
     2881 gctatttaga tttttgcact gcacttgccc gctttgcagt aacctacaat gcatccacgc
     2941 ctgcactttc ccggcaacca tatctgcggt tgtttaatgc aaaacatcct ttgcttgtga
     3001 caatgcatgg caaaaaaatt gtaccatgcg atattgaatg cggcatcaat tttaaatgtc
     3061 ttatcatatc gggcgtaaac accggcggga aaacagtgct tttaaaaatg ttagggcttt
     3121 ttgctcttat ggtgcgatac ggactcccta tcaccgccag ccctgattca acagcaggat
     3181 attttgaatc agtgtatgct gacattggtg atgaacagaa tattatgcag tcgctgtcaa
     3241 ccttttccgg acagataaaa aatattgtac gcatactaaa ccttgctaat gaaaaaagcc
     3301 tggtgcttat tgatgagatt attgtaggta cagacccaaa acagggagct gcaatagcac
     3361 aagcagtgtt gcagtattta gtaaataaaa aagcggttgt agccgttaca acacattatt
     3421 cacagctaaa agaaattgct tctgttgata gccaatttga aaatgcatcc gttgtttttg
     3481 atatccatac catgcagccc acctatcagg tgctcatggg tattcctggg gcaagctata
     3541 ctattgaaat agcaaaaaat ttgtctttac cgcaaaaaat cattgatgat gccatatcct
     3601 tacttgacag cacctcgcta accgtggacg cactgctttc ccaaataacc cagcaatcgc
     3661 atacattaca tcaaaaagaa caggaacttg cactattaaa acaggagctt aatcaattaa
     3721 aaaatgacta cgataaaaag ataaatgagc tacagcaaca aatagaaaaa gtaacaaaag
     3781 agcaggcaat tgaatttgta catgaacttc atcaataccg aaacaccatc attgacaaaa
     3841 tacgcaccat gcattcaatg tccatgaagg atgcaacaca gcttgtaaat gacattacac
     3901 aaatgcagct ttcattgcag gatactatca aacagctcca gaaaccctct gaagattttt
     3961 taccctgtac tgttgataca gtgcaaaaag gttcaaaagt atttataaag ccactggaac
     4021 aatacggata tgttgaagat gttgatgaaa aaagcaagca gttgaaagta cgcatgggta
     4081 ctgtgaatag caggttttca tttgatgatg tgtattgtat tccggaaagc aaaaagctaa
     4141 ccactgttaa aacaaaaacc aatacaatag caacacagga tatccagcat ataccaatga
     4201 gcattcagac aaaatataat accattgatc ttcgtggttt atatgttgat gaagcattgc
     4261 aaaaaatgga tcatgaactg gataatcttt taaaaaacaa tatcccaacc gctattatta
     4321 ttcatggaca tgggacaggc tctttaaaaa aagctattcg ccagtacctt aagcattcgt
     4381 tttatgtaaa aggctaccgt cccggacagc cggaggaagg tggtgatggc gtaacagtag
     4441 tgcagttgcg atgagctttt cataaacaat acaacaacaa cagtatgtac tattaataaa
     4501 tgtaatgaat gactgcatgc atgtactaat attctactct tttattgtac ttattatatt
     4561 tattatactt tgataatcac cgcctcatac gcatcaagcg tgagagcacc cccatgtatt
     4621 gaagaatgtg aagaatgcga agaatgaatt gaagaatttc cacaggatga aagcaacacc
     4681 tcgccttcta acccaatggt tacatccttt tttgcttttg aaaaattcaa tgcaatcatc
     4741 aactgttcat tgttatggca tcgtttatac agcagcacat catccggtgc aggtacaaat
     4801 tcaatatcac ccgcatacag agcaggatgc aatcttctga tggcaataca atccttatac
     4861 aggtgtaata ttgactgctt gttaccctgc tgctgcaaaa cattagtggt tacataatca
     4921 ctattgaccg gcagccacgg tttaccgttt gaaaagcctg cattctgagt catatcccat
     4981 tgcatgggag tacgtgcttt atccctgcct ttgtgaaatg gccagtactt ctttcccagc
     5041 gggtcctgta tctggctgcg tttaatcttc ccatcaacca tacctatctc ttcgccgtaa
     5101 taaataaatg gcgttccctt ctgggttaac agcatcactg ctaacagctt tgccttctgc
     5161 acatcattat ttgccagccg tgttattgct cgcgactggt catgattgga cagtacatga
     5221 catggccagc cactatcgcc aatagcatgg taccaatttt ttaagatacg ttcaaactcg
     5281 cgtgcgttaa agcgtgcgta gatagttgaa aaatcaaatg acagatgcaa ctcatcatta
     5341 ttacccaggt acgatgcagc catcgctggc tctttttcac cttcgtttgg atatgtttca
     5401 ccaacgctca tagtatcacc ataactatct aacaattgac gtaactcttt caggatatca
     5461 tgtatctcag gctgattgcg atcatatata tgaacctgca aatcatgacg tcgtggatag
     5521 gttaaacgta acctataggg attgcttcgt acattaatat ccttaacaat atagttgatt
     5581 acatcaaatc gaaaaccctt tacacccatg tcaagccagt agcgcagtac atcaaccatt
     5641 gcttttttaa cttcaggatt atgccagttc aaatcaggct gctgctcggt aaaaagatga
     5701 agatagtagt gacgcgtcaa ctcatcccac gtccatgctc taccgccgaa tgcagccatc
     5761 cagttattgg gcactttacc attttttccg gggtgccata tataccaatc acgctttgga
     5821 ttatcaactg atgaacgtga ttccacaaac cacggatgca ggtgcgatgt atggttcaac
     5881 accatatcca tgatgatagc aatgttgcgt ttttcggctt cttttaataa ttttttaaaa
     5941 ttggcgatag ttccaaatat cggatctatt gaacaatagt cactcacatc gtaaccaaag
     6001 tcatacattg gtgagcggta aataggcgat agccatatac catcaacacc tacatcctga
     6061 agataatcaa gcgattggat gataccctcc agatcgccaa tgccatcatt atttgaatcc
     6121 ttaaagctgc gtggatatat ttgatacagt acaccgtgtt tccaccagct ctttgttatg
     6181 ggtttactca taatctcttt cttttgattc ttttgaacct tgcttagatg gtgtagttat
     6241 agtttccatc tgagctttta catcatcccc cttgcctact gcctttaacg ctttaaaaaa
     6301 tgcagcagcg gtttcatata ctgccttatc aacacgatcg cgtatctttg cattgttata
     6361 atattcatca ttcattgcat ctttaaacgg tttgaagagc tttttcatat tgccgccctt
     6421 catgttattt gccagggcat actcttccat ctttttccac agttccatgg atatgccttt
     6481 atcagggtct ttttcgtacg taggaccttt ttcaactgct actccatatt catccatatc
     6541 tatcccccag ataacgttct gccacagcgt accaacatta gctttaaaaa tgccatacct
     6601 gtaaaactgc ccaacgtctt caatggatgt accggtggtg ccatgctgtg caataccaac
     6661 accccatggt gacatagccc tgtaggtatt gtgagttaat tgtagatcaa tattaccacg
     6721 aacattgcca tgagttgttc cattattgat ggcaattaaa tcagggcatt gccccaattt
     6781 tgtcaatgcc tctacaagtg tcactgcttc acttaccgaa gaattgccat gagctcctcc
     6841 aatctcgcca acctcggatt catacattaa accagctcgt ataacctgct cacttagcaa
     6901 tatgctcaat gcaatattct gttccatcgg caaaaatgaa acatcaattg aaaatgaacc
     6961 atatcctgcc tctattgaag cttttattat tgcatcggct ttatcaaacg ccttacggct
     7021 cacctcctga acagtggtat ggtctctgtg gcatgtaaac ggcactgcct tttcacctgc
     7081 attgcccatc ttgtcattta agctgacaac cagtttaata aaatctgcag gtgtaagccc
     7141 tgtatagcca cctgacaggt cgctttctga aagagccagt tcatatataa ccggtgcatt
     7201 caactccatc gatgccttta taatccctgg aatagcaatt tcatggcgtg tattgacagc
     7261 catagctacc cagtggtttc cttccttcat tgcggatttt atgattgatt tgccattgac
     7321 atgcatcact tttgaatcgg gaaatgcttt tttaaagttt tctggtcgta aatcaagaat
     7381 attcatagtt ccccctgatg tgtattgtaa tatttgctaa taccgtaata ctatcaatta
     7441 tttattcaag taaaatttgc cgtgataatt acatttttgc aaaagattgt atgctatatc
     7501 atataacaag ttatagctat acatggagtt accctattat tacataatta tttactatat
     7561 tatgaatttt tgtaatataa ttgaagcctc tgaacaaaaa taaaaattct aaaaaatcat
     7621 acttccaacg c
//
LOCUS       DAOOJH010000109         7579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_8188_length_7579_cov_9.404439, whole genome shotgun sequence.
ACCESSION   DAOOJH010000109 DAOOJH010000000
VERSION     DAOOJH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8188_length_7579_cov_9.404439"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(335..1036)
                     /locus_tag="PLJ75_10905"
     CDS             complement(335..1036)
                     /locus_tag="PLJ75_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05761.1"
                     /translation="MRLKLFILLFCIVAIVISYFYAPLVYYTYLKLYYQKICKIDEVA
                     FTNKVKDLYLSKQYDVLQQYLEHGAVLYPANKTIKKYNGLYLIDNGDVRGAFILIALD
                     AIPDDANTLEKIITVLDDNKSYQELLMIVHKKGIKTATIEFLYGKALCATGIYHQALS
                     HLKKAYTHGIVEADYYIGMCYHHLRDYNNALIWYKKALARKPGTVEYIKSIATVYQQK
                     GDYTSSTKYIMRLQH"
     gene            1318..>1532
                     /locus_tag="PLJ75_10910"
     CDS             1318..>1532
                     /locus_tag="PLJ75_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HPD05762.1"
                     /translation="MEQNTAKLEDYKRIYPEKFLPEEKIFSHINRGDRIFIGTACSEP
                     QYLVNALVEYVKSYPKAFFDTEVIHVWT"
     assembly_gap    1533..1632
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1633..3093
                     /locus_tag="PLJ75_10915"
     CDS             <1633..3093
                     /locus_tag="PLJ75_10915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013323560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD05763.1"
                     /translation="GVSVDIVKTAVESARLVVVQVNKQMPRVHGDAFIHIKDVDFIVP
                     HDEEILEFTTKDSDDISNRIGKYVSLIIQDGDTIQVGYGRMPNAILKHLIGKKNLGVH
                     TELISDGIVELMKAGVVTNSEKTVDRGKTIASFCMGTKPTYEYIHDNPSIEFKTIDYT
                     NNPLIIAQQRRMTAINAALQIDLTGQATAESLGKSFFSGIGGQADFMRGAILAPGGKS
                     ILALRSTTEDEQTSRIVPFIPEGAGITLNRGDVHYVVTEYGIAYIHGKNIRERAMDLI
                     AIAHPKFRPWLIEEAKKDGLIYRDQAFIPGKKGEYPEHLETFRTTKTGIKLFLRPAKI
                     SDEELLKDFFYSLSDQSLYRRFISTRKDMPHERLQEMVVIDYTKELVILAVVEQNGKE
                     VVVGVGQYGIQEYQHWAEVAFAVRDDYQYKGIGQVLLSYLTLIAKRNGLLGFTAEVLV
                     ENKPMLHLFEKMGFNIEKRSSAGVYELKMEFRGDEQ"
     gene            3090..5207
                     /locus_tag="PLJ75_10920"
     CDS             3090..5207
                     /locus_tag="PLJ75_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPD05764.1"
                     /translation="MKSKNSSIVTLFQPKSVAVVGASSQPGKIGYSITRNIIQGGFKG
                     RIYPINPKGGAILGVDCYPSISDIKDEIDVATICVPAKLTFDAVKECADKGVKHVQII
                     TSGFSEIGEVELEHKIVEYARSKGTRILGPNIFGVYSASGNYNSTFSATEILRGNVAI
                     LTQSGALGIAMIGKTAVENMGLSAIVSIGNKADIDEADCLEFVMRDQETKVILMYIEG
                     IKEGERFIEAVREATKLKPIVVLKSGRSKRGAMAAASHTGSLAGSDEIFDAIARQSGL
                     LRAENLQEAFNWCKFLAYSPRPKGKNTVIVTNGGGIGVMATDACEKYGVDLYDDQLVL
                     KEVFGPATPEFGSTKNPVDITGGAKAADYDLALSAPTRSEDMNATLALYCETATFDAE
                     NLAPMIRDTYGKHLASGKPVAYAVVGGRSVEDAIVQLRKMNVPVFPDVYEAVSCMGAL
                     YRYTTMLAERDDRVDDVDMDINTINKIIDGAIADGRTFLLANEGQEVMKASGVMIPGA
                     GIGKSIDECVRLAEQIGYPVVMKVVSRDILHKSDAGGVALDILNKQEVMDAYEAIMKN
                     CRAYKEDAVIDGIEVCEMVQKGVELIVGARRDPQLGPIVMCGLGGIYVEVMKDVAFRA
                     ATLNKKEARSMLSEIRSYPLLLGVRGEEQKDIDIVVDTIIKVATIIRKIPRITDIEIN
                     PVVVYEQGKGLKAVDVRIIISKS"
     gene            5220..6251
                     /locus_tag="PLJ75_10925"
     CDS             5220..6251
                     /locus_tag="PLJ75_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD05765.1"
                     /translation="MNKIVFACTRRSAGKTTIFTGLAKAMGKEVAYMKPFGDRLIYRK
                     KRLWDYDAALLTNLFGLEENPEDMTIGFEHAKLRYVYDEARIKEKLLEVAKSIAKNRE
                     VLFIESGKDLAYGVSVYLDPITVAKSLDAKLVLIVGGNDDVIIDDITLFKRYVDTSDI
                     NFLGVIINKIHDLDDFKSTYADKIEKMGVPILGMIPYSAELTYFSMDYLSEALFAKVI
                     AGESGLNKVVKNVFLGAMSVNEALRNPLFNKEGKLLITSGDRSDIIISALETNTVGIV
                     LTNNILPPSNIISKAAEKNIPLLLVSSNAYQVAGQIDKMEALLTKDNKEKIALAEKLV
                     KEHVNISAL"
     gene            6453..6821
                     /locus_tag="PLJ75_10930"
     CDS             6453..6821
                     /locus_tag="PLJ75_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05766.1"
                     /translation="MASPFILQNMYDSSLYICQERIWLQIIDLAFARGFNPVGTRLDY
                     YYELEQVWEEDMPYAEKIFTAIMIHVRCLNWDGCNFKDRENQIVSDDDCSELLYILND
                     TLPHDLKDFFKKGSFRICSE"
     gene            6881..>7579
                     /locus_tag="PLJ75_10935"
     CDS             6881..>7579
                     /locus_tag="PLJ75_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPD05767.1"
                     /translation="MGIVEWKKDENIAIITMNTVENRHNVMFAQAMLQALDEAEADRE
                     INGIVLTSSDAKNFSLGVDIEWLMPRMAEKDFKAIKDFMYGMNNVFKRLLLTPMPVIA
                     AINGHAFGNGAILSCACDFRFMRSDRGFFCFPEVDLGIPFLPGMIAFVKKALPYYKFN
                     EMKLTGRRVPAPELAEHHIIEKACANVDELMNESIAFAKSFKKKRGIFGEHKKRLHKH
                     IIEVIDKEDPEFIES"
BASE COUNT         2399 a         1284 c         1564 g         2232 t
ORIGIN      
        1 ggatacactt tgcctgtata accagcacaa acattgcgca gaatataaaa accatctgat
       61 ccagcaatcc cactggcacc tataattgcc actccatcag gatgaaaaaa ataatcaagt
      121 tcatgcttca tagcttcccc caccaaaaat agaattattc ctcaatgtta ttccaggctt
      181 aacccgtaat cctttttgct gctccagtca ttccgggctt tatcctgaat caagatattc
      241 atggatcatg gctcaggata atatccgcca catagtttcg tctcatttga gtgagtacag
      301 cacagcatag cttttgcaaa caccccattg atcattaatg ctgcaacctc atgatatatt
      361 ttgttgatga tgtataatct cctttctgct gatatactgt tgcaatgctt ttaatatatt
      421 ctacagtacc aggtttgcgc gctaatgcct ttttatacca tatcagagca ttgttataat
      481 cccttagatg atggtagcac atcccaatgt agtaatcagc ttctacaata ccatgagtgt
      541 aggctttttt taagtgcgat agtgcttgat gatatatccc tgttgcacac aaagctttcc
      601 catataggaa ttctatagta gcagtcttta ttcccttttt atgaacgatc atgagtagtt
      661 cttgatacga tttattatca tcaaggactg ttataatctt ttccaatgta tttgcatcat
      721 caggaattgc atccaatgca ataagtataa aagctccccg tacatctcca ttatcaatca
      781 ggtacaatcc gttatatttt tttattgttt tatttgctgg atacagtacc gcgccatgct
      841 caagatattg ttgcagaaca tcgtattgtt ttgataaata taaatccttt actttattgg
      901 taaaagctac ttcatcgatt ttgcaaatct tctgataata taatttcaaa taggtgtaat
      961 ataccagcgg cgcataaaaa tatgagataa ctatcgccac aatacaaaat aacaatataa
     1021 acaatttcaa ccgcatattt aattaccagc cattaacctg acgaatatct gtatatcatt
     1081 cattttcgtc aacataaaaa cattataccg tgctattgct tcatttcaaa gcctttatta
     1141 attattcctt gacatgtcaa aaaatgatag atagattatt aatataataa tcaaatattg
     1201 accatactga ttattatatt ttatttacta taaaaatttg aattgacaga attaatggct
     1261 aaaatatttt atatacaatc tgctattttt tatacatagc agatgaaaag ggggagtatg
     1321 gagcagaata ctgcaaaact ggaagattat aaaagaatct accctgagaa atttcttcca
     1381 gaggagaaga tattcagtca tattaaccgt ggcgacagaa tcttcatagg cacagcatgc
     1441 agtgaacctc aatatctggt caatgctctt gttgaatatg taaagtcgta tcctaaagct
     1501 ttttttgaca ctgaggtgat tcatgtatgg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnggcgttag tgttgatatt gtaaaaactg ccgtagaaag tgcacggctt
     1681 gttgttgtcc aggttaacaa gcaaatgcct cgcgttcatg gcgatgcatt tatacatata
     1741 aaggatgttg attttattgt accccatgat gaagaaatat tagaattcac aaccaaagat
     1801 tcagatgaca tttcaaaccg cattgggaaa tatgtttcac ttatcattca agatggtgac
     1861 accattcagg ttggatatgg aagaatgcct aatgctatcc tgaaacatct gataggcaaa
     1921 aaaaaccttg gtgttcatac cgaacttatt tctgatggca ttgtcgaatt gatgaaggca
     1981 ggcgttgtta ccaattctga gaagacagtt gaccggggca aaaccattgc atcattttgc
     2041 atgggcacaa aacctaccta cgaatatatt catgacaatc catctattga gtttaaaaca
     2101 attgattaca ccaataatcc gctcatcatt gcgcagcaac gcaggatgac tgcaattaat
     2161 gctgcactgc aaatagattt aaccggtcag gctactgctg aatcgctggg gaaaagtttt
     2221 tttagcggta tcggtggaca ggctgacttt atgcgaggtg ctatacttgc tcctggcggc
     2281 aaaagcatcc ttgcattacg ttcaaccaca gaagatgaac agacttcacg aattgtgcct
     2341 tttatacctg aaggagcagg gattaccctg aatcgtggtg atgttcatta cgtggtaaca
     2401 gaatatggga tagcttatat ccatggcaaa aacattaggg agcgtgcaat ggatctgatt
     2461 gcaatagctc atccaaagtt caggccgtgg cttatagaag aagccaagaa agatggtctt
     2521 atttacagag atcaggcatt tatcccgggc aagaagggtg aataccctga gcatcttgag
     2581 acattccgta caacaaagac aggtataaag ttatttttac gacctgccaa gatcagcgat
     2641 gaagaactat taaaggactt tttttactca ctatccgacc agagcctcta caggcgtttt
     2701 atttcaacac gaaaagatat gccacatgag cgtttacagg agatggttgt cattgattat
     2761 accaaagagc tggtaatttt agctgttgtt gagcagaatg ggaaagaagt tgtggtcggc
     2821 gttggtcagt acggtataca ggaatatcaa cactgggcag aagtagcatt tgctgtgcgt
     2881 gatgattatc aatataaagg tattggtcag gtattgctat catatttaac actgatagct
     2941 aaaagaaatg gtttgctggg attcactgca gaggtgcttg ttgaaaacaa gccaatgctg
     3001 catctttttg aaaaaatggg gtttaacata gaaaagagga gtagtgcagg agtatacgaa
     3061 ttgaagatgg aattcagggg agatgaacaa tgaaatcaaa aaattctagt attgttactt
     3121 tattccaacc caaaagtgtc gcagtagttg gtgcttcgtc acaaccaggc aagattgggt
     3181 acagtatcac ccgtaatatc atacagggtg gttttaaagg caggatctac cctataaatc
     3241 ccaaaggggg tgcaatatta ggtgtagatt gttacccaag tatttcggat attaaggatg
     3301 aaattgatgt agcaactatt tgtgttcctg caaaattaac ctttgacgca gtcaaagagt
     3361 gcgcagacaa gggtgtaaaa catgtgcaga ttattacctc gggattttca gaaattggtg
     3421 aggttgaact tgagcataag attgtagaat atgcgcgttc aaaaggaaca cgtattttag
     3481 gtcctaacat ttttggagtc tactctgcat cgggcaatta caactcaaca ttttcagcta
     3541 cagagatttt acgtgggaat gttgctatat tgacccagag cggcgcactg ggaattgcaa
     3601 tgattgggaa aactgctgtt gaaaatatgg gattatctgc tattgtatca ataggcaaca
     3661 aagccgacat tgatgaagct gattgtctgg aatttgttat gcgcgatcag gaaacaaaag
     3721 tcattcttat gtatattgaa ggtattaaag aaggcgaacg ttttattgaa gctgttcgtg
     3781 aagcaacaaa gctaaaacca atcgtagtgt taaaatcagg acgttcaaaa cgtggtgcaa
     3841 tggctgcagc atcacatacc gggtcattgg ctggttcaga tgaaatcttt gatgctattg
     3901 cacgacaatc aggattgctt agagcagaaa atttacagga agcattcaac tggtgcaaat
     3961 ttttggctta ttcgcctcgt ccaaaaggca aaaatacggt tattgttacc aatggcggtg
     4021 gaattggtgt tatggcaaca gatgcatgcg aaaaatatgg tgttgattta tatgatgatc
     4081 agcttgtact aaaagaagtt tttgggcctg caacaccaga atttggttcc acaaaaaatc
     4141 cagttgatat taccggtggt gctaaagctg cagattatga tttagctctc agtgcaccta
     4201 cccgttccga agatatgaat gcaacgttag ccctttattg tgaaacagca acatttgatg
     4261 ccgagaactt agcgccaatg attcgtgata catatggcaa gcatctggca agtggaaaac
     4321 ccgttgcata tgccgttgta ggtggtagat cagttgaaga tgcaattgta cagctcagga
     4381 agatgaatgt ccctgtattt ccggatgttt atgaagctgt ttcctgtatg ggtgcattat
     4441 atagatatac aaccatgtta gcggaacgcg atgatagagt ggatgatgta gatatggata
     4501 ttaacacaat taataaaatc attgatggag caattgccga tggcagaaca ttcctgcttg
     4561 ctaatgaagg acaggaagta atgaaagctt ctggtgtcat gataccaggc gcaggtattg
     4621 gtaaatcaat tgatgaatgc gtgcggctgg ctgagcagat agggtatcct gttgttatga
     4681 aggttgtttc tcgtgatatt ttacataaat ctgatgctgg tggcgtagca ttagacatat
     4741 taaataaaca ggaagtaatg gatgcatatg aagctataat gaagaactgt agggcataca
     4801 aggaagatgc tgttatagac ggtattgaag tatgcgaaat ggttcagaaa ggtgttgaac
     4861 tcattgttgg tgcacgcaga gatccacagc ttgggccaat cgttatgtgt ggattgggtg
     4921 gtatctatgt tgaagttatg aaggatgttg cctttagagc tgcaaccctt aacaaaaaag
     4981 aagcacgttc aatgctatct gaaatcagat catatccatt gctccttgga gttcgcggtg
     5041 aagagcaaaa agatattgat attgttgttg acacaataat taaagttgca actatcatca
     5101 ggaagatacc gcgaatcact gatatcgaga ttaatcctgt tgtcgtttac gagcagggca
     5161 aagggcttaa agcagtagac gtcaggataa ttatttctaa atcgtaagcg aggtatatta
     5221 tgaataaaat agtatttgct tgtacacgaa gaagcgccgg aaaaactaca atctttaccg
     5281 ggttagcaaa agcaatgggc aaagaagttg catatatgaa accctttggc gataggctta
     5341 tatacaggaa aaaacggcta tgggattatg atgctgcatt acttaccaat ctttttggat
     5401 tggaagaaaa tcccgaagat atgacgattg gttttgaaca tgctaaactg cgatatgtgt
     5461 atgatgaagc ccgtatcaaa gaaaagcttc ttgaagttgc aaaatcaata gcaaagaaca
     5521 gggaagtgct ctttattgaa agtgggaaag accttgccta tggtgtttca gtatatttag
     5581 acccaataac agtggcaaaa tcacttgatg caaaattagt gcttattgtt ggtggcaacg
     5641 atgatgttat tattgatgat ataacactct ttaagcgata tgtcgatacc agcgatatca
     5701 atttcttagg tgtgatcata aacaagatcc acgatcttga tgatttcaaa agcacctatg
     5761 cagataaaat tgagaagatg ggtgtgccca ttttaggcat gattccctat tcagcagaac
     5821 ttacctattt ttccatggac tatctgtcag aagcactatt tgcaaaggtt attgctggcg
     5881 aatcaggatt aaataaagta gttaaaaatg tatttctggg cgccatgtcg gtaaacgaag
     5941 cactgagaaa tcctctcttt aataaagaag gtaaactttt gattacaagc ggcgatcgaa
     6001 gtgatattat tatatcggca ctggaaacaa atacggtagg gattgtctta accaataata
     6061 tcctgcctcc atcaaatatt atttcaaaag ccgctgagaa gaacattcca cttctccttg
     6121 tttcaagcaa tgcctatcag gtggcaggac aaatcgacaa gatggaagct cttcttacaa
     6181 aggataacaa ggaaaagatt gcactggctg aaaagctggt aaaagaacat gtaaacatct
     6241 ccgctctata atatgttaca tatttcatgt ataaaaaaag ggctattctt caatagccct
     6301 ttttttatta ataaatcgat gaacagcaat catctaattt ttaatgatac taccattacc
     6361 ggtaactatc gctcaaattt taattattaa aacataaaac agccttgaca gcacataacg
     6421 atgttacgca tatagtgtta tgaaggtgta ctatggcctc tccatttatt ttacaaaaca
     6481 tgtacgatag ttccctgtat atatgtcagg aacgtatatg gcttcagatt attgatttag
     6541 cgtttgcccg tggatttaac ccggtaggga cccgacttga ttattattat gaactagagc
     6601 aggtatggga agaggatatg ccatatgccg aaaagatttt tactgcaata atgatacacg
     6661 ttcgatgctt aaactgggat ggatgcaact tcaaagacag ggaaaatcaa atagtaagcg
     6721 atgacgattg cagcgaactt ttatatatat tgaatgacac attgcctcat gatttaaaag
     6781 atttttttaa aaaaggatca ttcagaattt gcagtgaata attgttacat ataatgcgca
     6841 cttactatta ttttttaaat aaattttaca gggggtatat atgggcattg tagaatggaa
     6901 aaaagatgag aatatcgcaa ttatcactat gaacactgtt gaaaacagac ataacgtcat
     6961 gtttgctcag gcaatgttgc aggcactgga cgaagctgaa gctgacaggg aaattaacgg
     7021 gatagtattg acttccagtg atgcaaaaaa ttttagcctt ggcgtagaca ttgagtggct
     7081 tatgccaagg atggcagaaa aagattttaa agctattaaa gatttcatgt atggtatgaa
     7141 caatgtattt aagcgtctgc tccttacgcc catgccggtt attgctgcca tcaatggaca
     7201 tgcatttggt aatggcgcta tactatcctg cgcatgtgat ttcaggttta tgcgttcgga
     7261 tagaggtttt ttttgtttcc cggaggttga tttaggtatc ccctttttgc ccgggatgat
     7321 agcatttgtt aaaaaagccc ttccctatta taaatttaat gagatgaaat taacagggag
     7381 aagagttccc gccccagagc ttgctgaaca tcacattatt gaaaaggcat gtgccaatgt
     7441 tgatgaactg atgaatgaat caattgcatt tgctaaatcc ttcaagaaaa aacgtggtat
     7501 atttggtgag cataaaaaac gattacataa gcatatcatt gaggtgattg ataaagagga
     7561 ccccgaattt attgaatcg
//
LOCUS       DAOOJH010000110         7255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_8616_length_7255_cov_9.742222, whole genome shotgun sequence.
ACCESSION   DAOOJH010000110 DAOOJH010000000
VERSION     DAOOJH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8616_length_7255_cov_9.742222"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..656
                     /locus_tag="PLJ75_10940"
     CDS             63..656
                     /locus_tag="PLJ75_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPD05768.1"
                     /translation="MKRLHYLLPVFIIAFVFSFSGCGYNKLQMLEEEVFKSYGDLESA
                     LQRRADLIPNLVATVKGYAAHEKQTLQAVIEARAKATSVQLSPKDLSNPEAIAKFQAA
                     QGELSNALGRLMVVIERYPNLKADAHFTDLMHQLEGTENRINVARQRYNEAVKDFNYN
                     IRMFPYNLTNMILLHLERKEYFKAEEGAKAAPEVSFD"
     gene            668..1327
                     /locus_tag="PLJ75_10945"
     CDS             668..1327
                     /locus_tag="PLJ75_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPD05769.1"
                     /translation="MNNLVAQYITQADAQQIISTVKKVEKKTSAEIVPMIVNASFHYP
                     LASVYAATALSLPVSIALTHYIAPLFYYSRDNMWIFIALFSLLFLLFKGTIARNIFLL
                     RLFLNPHEVEAEVKEAATTAFYKNGLYKTADKNGVLIYISLLEKRVWILADEDAHKKI
                     GQEFWDSCVATIIDGVKKKQFVPVLCTVIETIGNKLSPLYPCKSDDIDELKNIIIGDS
                     Q"
     gene            1324..2166
                     /locus_tag="PLJ75_10950"
     CDS             1324..2166
                     /locus_tag="PLJ75_10950"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPD05770.1"
                     /translation="MRWFVTVCLTIIMFGSSGYSQDSTTVSIPPLKGRINDYAGILNE
                     QTESTLETKLAQLEKEDSTQVVVATVSSIGDEDIESYSIRLAETWKIGQKGLDNGVII
                     LVAHDNRQVRIEVGKGLEGTFTDLLAGRIIDYIMIPNFKNGDYDTGISQAVDAIIDIV
                     KGTFDASTLEKKNSASDYVMYFWIAFCVMLVISSFGKLLSGILSAIILPIFGSFFSFG
                     FPVLIILAVVGFFAGFILASISRTQGGFFPGGGSSSGGFFSGGGGFSGGGGSFGGGGA
                     SGSW"
     gene            2252..3922
                     /locus_tag="PLJ75_10955"
     CDS             2252..3922
                     /locus_tag="PLJ75_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HPD05771.1"
                     /translation="MYLGLDVGGTHTDTVIIGNSGVIAKSKIATTHDNLLSCITDSFS
                     AVLANVDPSQIQRVNLSTTLTTNAIIQNKCEKVGMFVSGGPGIHPASYSIGNFYYIID
                     GQIDHRGTEITLLNLKQLDKAIKKCKKKGIKVYAVISKFSTRNPEHEQLIAQKLGDVD
                     FISVGHKISGNLNFPRRIATAYYNTAVWRLYNSFSYAVAESLRAWGVTAPVNILKADG
                     GTIPLHISQTMPVESILSGPAASVIGTMALCNITEDAVMLDIGGTTCDIALFADGAPL
                     IEKDGIRINDRNTLVRSLKTKSIGIGGDSVIKINGTTVTTGPERLGMSMAEGGNNPTI
                     IDALNVLKTINFMDTSRSMEGLLALGKKYRLSVDDIANAAITYAMDVIYNEVMAMIEE
                     VNARPVYTIYEFLQGKVIKPKTIYLIGGPAKTLSNLIARRFGLNVKVPQHCQVANAIG
                     AAVARTTVEIELFADTQRCRLLIPNLEVNTRIPAGYTLDNAIDDAKNYMIQYLTTLGV
                     DEKEREIEIVDTYSFNMVKGFETVGKNIRVRCQVKPGILHQCKDILQI"
     assembly_gap    3990..4089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4090..5043
                     /locus_tag="PLJ75_10960"
     CDS             <4090..5043
                     /locus_tag="PLJ75_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPD05772.1"
                     /translation="ARGFCNINIEAVMVEYIRENYPVKKIAIVDTDCHHGDGTQDIYW
                     HDKDILYISLHQDGRTLYPGTGAIHELGGPNALGTTINIPLPPNTSDEGFLMAMERVV
                     LPVLKDFNPDIIINSAGQDNHYSDPITNMNFSASGYAQLTDMLNADIAVLEGGYSIQG
                     ALPYVNLGITLAMAGLDYSYVHEPDYAIKDLTQDREITKYIKKLCDKVLQRYEECKKP
                     SFKLMPGNYDVRKKEIYYDTDNIHEMQLESIMVCDQCRGVLKIETTSSINPLCLGIEI
                     PSGACDQCINEGYRLLDHALAGNRYKYVQLIDRINKKYVMY"
     gene            complement(5040..7208)
                     /locus_tag="PLJ75_10965"
     CDS             complement(5040..7208)
                     /locus_tag="PLJ75_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPD05773.1"
                     /translation="MKRFVKNIASEREYVVAREKDVPVSQYYGEDTFNFDVMRQKLPA
                     DVCKKLMNCIKEADKLDTEIAQTVAHAMKEWAIDKGATHFCHWFQPMTGTTAEKHDSF
                     IELTEEGIPIERFSASQLVKGEPDASSFPSGGLRSTFEARGYTAWDPSSPAFILRNGI
                     GATLCIPTVFLSYTGEALDNKTPLLRSINVLNKSALNMMRLLGNDRATRILATLGPEQ
                     EYFLIDMDYYYKRPDLVLTGRTLIGAPPSKGQQLEDHYFGSIKERILSYMHDVEEELY
                     KLGIPAKTRHNEVAPAQYEIAPIFEEANIAVDHNHLIMETLKRVAQKHKLAALLHEKP
                     FAGINGSGKHCNWSLSDNFGNNLLNPGQSPQDNIQFLVFLTATIRAVYYHADLLRASI
                     ASSSNDHRLGANEAPPAIISVFLGEQLTKILYDIEQGVITKATNKAIIDMGLSTLPVL
                     SKDNTDRNRTSPFAFTGNKFEFRAVGSSQSIALPITILNTIVAESLDILADKIRTKSG
                     DVRQAAIDVIREEFKTVKPILFMGDNYSKEWEKEAAKRGLPNKKVTAEALEDLVTDKA
                     KELFNKYGVFSTVELEARYTIRLERYIKEIDIEAKTLLDMVQTKVIPAALRYQNSIAS
                     SCLRIKDLLGDRAPIDAQKQLCETIAVLSNDIIKEANKLARLIEKVTAMHDEIEQAKQ
                     YAYDVKSQMEALRSKVDLIETVASDNLWPFPRLWEMLFIS"
BASE COUNT         2118 a         1432 c         1455 g         2150 t
ORIGIN      
        1 aagatgtttg ccatgttgtt gttttgggaa tactaattga ttgtaattac aatggaggac
       61 acatgaagag gctacattac ctgctccctg tttttattat tgctttcgtt ttttcttttt
      121 ccgggtgtgg ctataacaaa cttcagatgc tggaagaaga ggtatttaaa tcctacggtg
      181 atttagaatc agcattacag cgcagggctg atcttattcc caatctggtt gctactgtta
      241 aaggctatgc agcgcacgaa aaacaaacat tgcaggcagt gatagaagcg cgggcaaaag
      301 ccacttcggt acaactatcg ccaaaggatt taagcaatcc cgaagccatt gcaaaatttc
      361 aggctgctca aggtgagctt tctaatgcgt taggccgtct catggtggtt attgaacgct
      421 atcccaatct taaagctgat gcccatttca ccgaccttat gcatcaactt gaaggcaccg
      481 aaaaccgaat caatgttgca cgccagcgtt acaacgaagc ggtaaaagac tttaattata
      541 atatcagaat gtttccctat aatcttacta atatgatttt actccacctg gaacgcaaag
      601 aatattttaa agccgaagaa ggagcaaaag cggctcctga agtttctttt gattagcgat
      661 agttactatg aacaatctgg ttgcacaata tattacacag gctgacgcac agcagattat
      721 tagcactgtt aagaaggtgg aaaagaaaac cagtgctgag attgttccca tgatagttaa
      781 tgcaagtttc cactatccgc ttgcaagcgt gtatgctgct acggcgctat cattacccgt
      841 atcgattgca ttgacccatt atatagcacc gctcttttac tatagcaggg ataatatgtg
      901 gatttttatt gcattgtttt cactgctgtt ccttttattt aaaggaacta tcgcccgaaa
      961 tatttttctt ttgcgccttt ttttaaatcc gcatgaggta gaagctgagg taaaagaggc
     1021 tgccaccact gcattttata aaaatggttt atataaaaca gccgataaaa atggcgtact
     1081 tatttatata tcactgctgg aaaagcgtgt ctggatatta gccgatgagg atgcacataa
     1141 aaagattgga caggaattct gggatagctg cgttgccacc attattgacg gagtaaagaa
     1201 aaaacagttt gtacctgttt tatgtactgt tatagaaaca attggcaaca aattgtcacc
     1261 attgtatccc tgcaaaagtg atgatattga tgagcttaaa aatataatta ttggtgatag
     1321 ccaatgagat ggtttgtaac tgtatgtttg acaatcatta tgtttggttc ctctggctat
     1381 tcacaagata gcaccacggt ttcaattcca cctttaaaag gccggattaa tgactatgca
     1441 ggtatcctca atgaacaaac cgaaagtaca cttgaaacaa agttagctca actggaaaag
     1501 gaggattcga cgcaggttgt tgtagcaacc gtatcctcaa ttggtgacga agacattgaa
     1561 tcatattcta tacggttagc tgaaacatgg aaaattgggc agaaggggct ggataacggg
     1621 gttatcattt tagtagcaca tgacaaccgc caggtgcgta ttgaggttgg gaaagggctt
     1681 gaaggcacct ttaccgacct tttagcaggc aggattatag attacatcat gataccaaat
     1741 ttcaaaaacg gcgattatga taccggcata tcacaggctg ttgatgctat cattgacatt
     1801 gtaaaaggta catttgatgc cagcacatta gaaaaaaaga attctgcttc tgactatgta
     1861 atgtatttct ggattgcatt ttgcgttatg ctggtaatta gttcatttgg caaattgctc
     1921 agcggcatat taagtgcaat tatccttcct atttttggtt catttttttc gtttggattt
     1981 cctgtactta taatccttgc cgttgtgggt ttctttgcag ggtttattct tgcttcaata
     2041 tcgcgtacac agggtggttt tttccccggt ggcggaagtt catcaggcgg ttttttttca
     2101 ggtggtggag gcttcagcgg aggcggcggc agctttggcg gcggcggtgc ttcaggtagc
     2161 tggtaaatca taaaaaaaga gattattatt taaaggatac gatcttacaa ttattttttt
     2221 gtttgatagt ttatataata aaggacttgc aatgtattta ggacttgatg ttggcggtac
     2281 acatactgac actgtaatta ttggcaattc aggagttatt gcaaagtcaa agattgctac
     2341 cactcatgat aatctattaa gctgcataac agattctttt tccgcagttt tggccaatgt
     2401 tgatccatcg cagatacagc gtgtcaattt aagcaccact cttacaacaa atgcaattat
     2461 acaaaataaa tgtgaaaaag ttgggatgtt tgtcagtggt ggtcccggca tacatcctgc
     2521 atcatatagt attggcaact tctactatat catagatgga cagattgatc accggggtac
     2581 tgaaataaca ttgcttaatc taaaacagct ggataaagcc ataaagaagt gtaagaagaa
     2641 aggcatcaaa gtatatgcag ttatttctaa attttccact cgcaatcccg agcatgagca
     2701 attaatagca caaaaattgg gtgatgttga ttttataagt gtagggcata aaatctcagg
     2761 gaacttgaat tttccacggc gtatagcaac cgcgtattac aacacagcag tatggcggct
     2821 gtataattca ttttcatatg ccgttgcgga aagcctccgt gcatgggggg tgacggctcc
     2881 tgtcaatata ctcaaagctg atggcggcac catcccgttg catatctcgc aaaccatgcc
     2941 ggtagaatca attttatccg ggcctgctgc cagcgttata gggaccatgg ctctgtgtaa
     3001 cataactgaa gatgctgtaa tgcttgatat tggtggcaca acatgtgaca ttgccctttt
     3061 tgccgatggt gcaccattaa ttgaaaaaga tggcatacgt atcaatgaca gaaatacgtt
     3121 agtacgttcg ctcaaaacaa aatcaattgg aataggcggt gattcggtca ttaaaataaa
     3181 cggcactacc gttactaccg gacccgaacg gcttggtatg tcaatggcag aaggcggcaa
     3241 caatccaact attattgatg cattaaatgt attgaaaaca ataaacttta tggatacatc
     3301 acgctcaatg gaaggattac ttgcgctggg gaaaaaatat cgtttaagtg ttgatgatat
     3361 agcaaatgca gcaattactt atgccatgga tgttatctat aatgaagtta tggcaatgat
     3421 agaagaggtc aatgcaagac ctgtttatac aatatacgaa tttttacagg gcaaggttat
     3481 taaaccaaaa actatctacc ttattggtgg acctgcaaaa actttatcaa accttattgc
     3541 ccggcgtttt ggattaaacg ttaaagtgcc acaacactgc caggtagcta atgccattgg
     3601 tgcagcagtt gcccgcacta ctgttgagat tgagctcttt gcagacacac agcgttgccg
     3661 ccttcttatt cctaatttag aggtaaacac ccgtattccg gctggctata ctcttgataa
     3721 tgctattgac gatgccaaaa actatatgat acagtattta acaacactgg gagttgatga
     3781 aaaagagcga gaaatagaaa tagttgatac gtattccttc aatatggtaa agggatttga
     3841 aactgtaggg aaaaatatcc gtgtgcgatg tcaggttaaa cccggtattt tgcatcaatg
     3901 taaggatata ttgcagatat gaaagcacaa tcatcattag gtgttgtatt tttcccggct
     3961 tttgactggg caatacatcc tcttcatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnng cacgcggttt ttgtaatatc aacattgaag cggttatggt tgaatatatc
     4141 cgtgaaaact atcctgtaaa aaaaattgct atagttgata ctgattgcca tcatggtgat
     4201 ggaacacagg atatttactg gcatgataag gatatactgt atatctcatt acatcaagac
     4261 ggaagaacgc tataccccgg taccggtgcc atccacgaat taggcggtcc caacgcactg
     4321 ggaacaacca taaacattcc actgccaccc aatacatccg atgagggttt tttaatggca
     4381 atggagcggg tagtattacc tgtattgaaa gacttcaatc ccgacattat cattaactca
     4441 gccgggcagg ataatcatta ctctgaccca ataaccaata tgaatttttc agcatccgga
     4501 tatgcacaat taacggatat gctcaatgct gacatagcag tacttgaagg aggttactct
     4561 attcagggtg cactgcccta tgtaaattta ggtattacac tggctatggc aggtcttgat
     4621 tattcatatg tgcacgagcc tgattacgca ataaaagatc taacccagga tcgtgaaata
     4681 acaaaatata ttaaaaaact gtgcgataag gttcttcagc gatacgaaga gtgcaagaag
     4741 ccttcattta agcttatgcc aggaaattat gatgttcgca aaaaagagat ctactatgat
     4801 acggacaata tccacgagat gcaacttgaa tctatcatgg tatgtgatca atgccgcgga
     4861 gtattgaaga ttgaaaccac ttcatcgata aaccccttgt gcctgggcat tgaaattccc
     4921 tcaggcgcat gtgaccaatg tattaacgaa ggctatcgcc tgcttgacca tgcgctggca
     4981 ggcaatagat ataaatatgt tcaactcatt gacagaataa acaagaaata cgttatgtat
     5041 taagaaataa acagcatctc ccacagtctt ggaaatggcc acaggttatc acttgctact
     5101 gtctctatca aatctacttt acttcgcaaa gcctccattt gagatttaac atcataagca
     5161 tactgttttg cctgctctat ctcatcatgc attgccgtta ccttctcaat taatctggca
     5221 agcttattag cttctttgat aatatcattg cttaatacag caatcgtttc acacaattgt
     5281 ttttgtgcat ctattggagc cctgtcaccc agaagatctt taatccgcaa acacgacgaa
     5341 gctattgaat tttgataacg caatgctgcc ggtattacct ttgtctgtac catatccaga
     5401 agtgtttttg cttctatatc aatttccttt atataccgtt ccagcctgat agtatagcgc
     5461 gcttccagct caaccgttga aaacacaccg tatttgttaa aaagttcttt agctttatct
     5521 gtaactaaat cctccagagc ctcggcagta accttcttat tgggtagtcc acgtttcgct
     5581 gcttctttct cccattcttt tgaataatta tcacccataa aaaggatagg tttcactgtt
     5641 ttaaactctt cccgtataac atcaatggct gcctgcctta catcgccgct ctttgttcta
     5701 attttatcgg caagtatatc aaggctttca gctacaatag tgttgagtat tgtaatcggt
     5761 aatgctatgg attgcgagga gcctacagcc ctgaattcaa acttattgcc ggtaaaagca
     5821 aaaggcgagg tgcggttgcg gtcggtatta tccttactta gcacaggcag tgttgaaagc
     5881 cccatgtcaa taatagcctt attggtagcc tttgttataa caccctgttc tatgtcatat
     5941 aaaatctttg ttagctgctc gcctaaaaat actgagataa ttgcaggcgg tgcctcattt
     6001 gcacctaatc ggtgatcatt gctgctggaa gcgatagatg ctctcagtaa atcagcatga
     6061 tagtacactg ctcgtatggt tgccgttaaa aaaactaaaa actgaatatt atcctgcggt
     6121 gattgaccgg gattaagcag gttattacca aaattatctg ataatgacca gttgcaatgc
     6181 ttccctgaac cattgatacc tgcaaaaggt ttttcatgaa gaagtgccgc aagcttatgt
     6241 ttttgggcaa cacgctttaa tgtttccata attaaatggt tatggtcaac agcaatatta
     6301 gcttcttcaa atattggagc tatttcatac tgggcgggag caacttcatt gtgacgtgtt
     6361 tttgcaggaa tccccaattt atataattct tcttccacat catgcatata tgaaagtatc
     6421 cgctccttaa tgctcccaaa ataatgatcc tccagttgct gtccttttga cggtggagcg
     6481 ccaataaggg tacgcccggt taacacaagg tccggacgtt tgtaatagta atccatatct
     6541 ataagaaaat actcctgctc aggaccaagg gttgctaaga ttcttgtggc tctatcgtta
     6601 cccagcaggc gcatcatgtt caaagcactt ttatttaaaa catttattga acgcagcaat
     6661 ggtgttttat tgtcaagagc ctcaccggta tatgacaaaa aaactgttgg aatacacagt
     6721 gtggcaccaa ttccattgcg taaaataaat gcagggcttg atggatccca tgcagtatat
     6781 ccgcgcgcct caaaggttga acgcaagcct ccactgggga aacttgaagc atccggttca
     6841 cctttgacaa gctggcttgc tgaaaaccgt tcaataggta ttccttcctc ggtcaattca
     6901 ataaatgagt catgtttttc agcagttgtc cccgtcattg gctgaaacca atgacaaaag
     6961 tgggtagcac ctttatctat tgcccattcc ttcattgcat gcgcaaccgt ctgtgctatc
     7021 tcagtatcaa gcttatcagc ttcttttata cagttcatca gctttttaca aacatctgcc
     7081 ggtaatttct gacgcatcac gtcaaaatta aatgtgtcct cgccatagta ttgagacacc
     7141 ggtacatcct tttcccgtgc cactacatac tcacgctcag atgcaatatt cttaacaaac
     7201 cgtttcatgg tatacctcgt tataatgtta gtgtaacagc tacaatgtat agcaa
//
LOCUS       DAOOJH010000111         7000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_8957_length_7000_cov_11.534773, whole genome shotgun sequence.
ACCESSION   DAOOJH010000111 DAOOJH010000000
VERSION     DAOOJH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8957_length_7000_cov_11.534773"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLJ75_10970"
     CDS             complement(<1..786)
                     /locus_tag="PLJ75_10970"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HPD05774.1"
                     /translation="MAQNIVAKVKDSIDKMPPLSPVVHKIIIVANDVTSSAQHLTDVI
                     QLDPVLTAKVIRMVNSAYFGLPQEIKSLKQAVVMLGINTIKNVALSSALMGKISLKKG
                     AIDAEEFWKHSIGVAVASKLIAQRLGIDKKLLEEFFIAGLIHDIGKVLLNNFFLDEMK
                     QIIEISQTKGGFIIDIEKNILGLSHEEIGIAIGKKWNFENNLLYAVGKHHIPIMKGDS
                     AIYSMIVHIADVFVKVLKVGFSGNYVIDLIDESIWQILNVNEEI"
     gene            complement(914..1261)
                     /locus_tag="PLJ75_10975"
     CDS             complement(914..1261)
                     /locus_tag="PLJ75_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPD05775.1"
                     /translation="MKQLFMIVLCITMFSCSEEQFGGLGIEVPAGPEKVGKNNPFIIV
                     KVYEGGTGHQAGLKEGDAIISVDGVPVDGLPCDYVVNNLLRGKVGSVVTLEIKRGETL
                     MLFRVLRGKIVLK"
     gene            complement(1295..1546)
                     /locus_tag="PLJ75_10980"
     CDS             complement(1295..1546)
                     /locus_tag="PLJ75_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05776.1"
                     /translation="MDNSELLNSIHRRIMNELLNHSNEKPGGQKLKEIVTIDQNLRKD
                     IADLYVQLSELGDKEMAINILSDHVAIMVEMIVHFKSEK"
     gene            complement(1615..>2640)
                     /locus_tag="PLJ75_10985"
     CDS             complement(1615..>2640)
                     /locus_tag="PLJ75_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05777.1"
                     /translation="DSAIRDNKEFIEFMDVPISNFGQKYKDQFKDIYEIHFNAEVAYL
                     QSDYNRAFKNVYESQKKQVNLYSLLVKDYLDDTRQFLDSISPEVIKSKNKKARLYLNL
                     AYRDREVARTHHTVADASNPKLCSNKIYKYIEAIKMVRRARRYGFLAIFESQNDETKK
                     KIYNHLFEIEREKGNVFYVRFISKTEQQIIDEMQKEYYDVQLPDITQQESKDEETYGK
                     RVEKRVRFKDERRVAEYLLTAEFEKAEPIIRDYVKDFNFNLIVATIEYLIANKSPIVA
                     GYSVEALKRYHDDDYSRYFNPSTIDSLAGEIKVVDTIPKKTGTETTEQQKEQIDKKEG
                     VTAEQNK"
     assembly_gap    2641..2740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2858..4288)
                     /locus_tag="PLJ75_10990"
     CDS             complement(2858..4288)
                     /locus_tag="PLJ75_10990"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPD05778.1"
                     /translation="MQGLELLTDLYELTMMQVYFKTNIINRYAIFDMFFRKNPFGSGY
                     VVFAGLEQLIAYLQNLSFSNDSIDYLYSLNIFSDDFLDYCKAFSFTGNIYSFQEGDIV
                     FPEEPIVRVEAELGQAQLIETALLNCINFQSLIATKSARICYAAKGRDVIEFGLRRAQ
                     GPDGGLSASRAAVIGGCVGTSNVLAGKMFGIPVKGTHAHSFVMAYDNEDEAFDHYTEV
                     FPDTSILLVDTYDTLGSGIKNAIAAAKKLAKKGKHLVGIRLDSGDLAYLSKQVRKMMD
                     EEGLQYIKILASNDLDEYKIDELLKAGAPIDLFGVGTRLATGFGESALSGVYKMAAIE
                     KDGKLIPKMKISDEISKGTIPSRKNVYRYYDSDNTIWFDLICQDEETVIKGDVYDIKG
                     NKFTLDGHMISRCMHAPIFHNGTLVYDKPTLQEIQHHCYNNMQSLTRSIKDIYDPVNY
                     DVYISPKLFSLLEKMRLSISDKKGLD"
     gene            4512..5501
                     /locus_tag="PLJ75_10995"
     CDS             4512..5501
                     /locus_tag="PLJ75_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05779.1"
                     /translation="MAFEPESDNLFSDHFIIIKGRFKALSMNLYGLFIIIFNQDKRID
                     TYNIAVSTFSNIYENEPHLPWDKYEQMILELKWKGNFNANLTSSIMDNFKEISAEDIK
                     TNQLYDYANSYDYNSLDTYLFDTINRVVHDKNLVLETSIQEVTAEEFAQTKEKRYNKA
                     EEKSTSETELEEGAVILNIQPILAPVKGKPIYELKIGDRIMTKIIPNTDRANYFIDLL
                     GLRVENHIKPIATEVIDIKSDGKSEPLEILTRIGPGIYGKCIEDERQVKLRLYDALID
                     GPLTQKVIPKRTQPSPTSVATYEDAGFSKLTYVIISLFVLLLIIFVLLIYISF"
     gene            5593..5721
                     /locus_tag="PLJ75_11000"
     CDS             5593..5721
                     /locus_tag="PLJ75_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05780.1"
                     /translation="MFYLLLRLLYEEMVEGKISREEYCEAVIRIASLHGFKRNGIQ"
     gene            5721..>7000
                     /locus_tag="PLJ75_11005"
     CDS             5721..>7000
                     /locus_tag="PLJ75_11005"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HPD05781.1"
                     /translation="MDNREYIMKTAHDNDVKFIRLWFTDILGFLKSFAITINELEDAL
                     EEGVRFDGSTIHGFVRKTENEMIAFPDIETFEILPWRPKSNSVARMFCYIYNVDMTPY
                     ECDVRRILYNNLKNAANRGYVFYVGPEIEFFLFRNAESTEFLDRGGYFDLTPLDIASD
                     IRRQMVLTLEEMGISVKSSHHEGAPSQHEIDLRHDDALTTADNIMTFKIATKEIAQQN
                     NMYASFMPKPLSHYNGSGMHIHQSLFKDDKNIFYDQHDENLLSQMAKCYIAGLLKHAP
                     EFFVLSNQWVNSYKRLHYGFEAPIDLSWSTNNPSSLVRITTSRTDKPNSVRVELRNPD
                     PACNPYLLFAAILASGLAGIDNNDVLPKPHDLTLKKHEPVAQPLPSTIEEALKKFDKS
                     QLMRQSLGNKLVDLFVENKYYELDQYNRHVTDYEL"
BASE COUNT         2219 a         1392 c         1052 g         2237 t
ORIGIN      
        1 aatctcttcg ttgacattaa gtatctgcca gattgactca tcaatgagat caataacata
       61 attaccacta aatccaactt ttaaaacctt cacaaaaaca tctgctatat gaacaatcat
      121 agagtatatg gctgaatcac ctttcattat tggtatatgg tgtttgccta ctgcatagag
      181 caggttattt tcaaagttcc attttttccc tattgctatg ccaatctctt catgagacaa
      241 acccagaatg tttttttcaa tgtcaataat gaaaccacct tttgtttgag aaatttcaat
      301 aatctgcttc atctcatcaa ggaaaaagtt atttaaaaga accttcccaa tatcatgtat
      361 aagccctgca ataaaaaatt cttccaatag ctttttgtca atacccaatc gttgagctat
      421 gagcttagag gcaacagcaa caccaattga atgcttccag aactcttcag catcaatagc
      481 cccctttttc aatgatattt tccccattaa ggctgaggat aaagctacgt ttttaattgt
      541 gtttatgccc agcatcacaa ctgcctgttt taaagattta atttcctgag gcagtccaaa
      601 ataagccgag ttaaccattc gtattacttt tgcagtaagc acaggatcaa gctgaataac
      661 atcagttaaa tgctgggcag acgatgtaac atcattagca acaatgataa ttttatgcac
      721 cacaggagaa agagggggca ttttgtctat tgaatcttta acttttgcaa caatattttg
      781 agccatcaag taccccaaac caaactgcat ttcaatgtta tactaacagc cagtaaataa
      841 tatcagctag tatcactacc cagaacaaaa tagaaaataa tataattatt ttattgattg
      901 catttttcat catttatttc agtactattt taccacgcaa aactctgaaa agcatgagcg
      961 tttcaccacg ttttatttca agggttacca ccgaacccac cttccctctg agtaaattgt
     1021 taacaacata atcacatggc agaccatcaa caggaacacc atcaactgaa attattgcgt
     1081 ccccttcctt cagtccagcc tgatgtcctg taccaccttc atataccttg actataataa
     1141 acggattatt tttccctacc ttttcagggc ctgcaggtac ttctatacct aatcctccaa
     1201 attgctcttc agaacaggaa aacattgtta tgcacaaaac tatcataaaa agttgcttca
     1261 tgttattccc tcattttaac atatctgtcg taacttattt ttcacttttg aaatgaacta
     1321 tcatctcaac cataatggca acatgatcag acaaaatatt tattgccatc tctttatcac
     1381 ccagctctga aagttgaaca tacaggtcag ctatatcttt tcgtaagttc tggtcaatgg
     1441 tcactatttc ttttaatttc tgcccacccg gtttttcatt tgagtgattt aatagctcat
     1501 tcataatacg cctgtggatt gagttgagta gttctgaatt atccatgacg atcccttcct
     1561 gttcatatta aattaatgta taacgttagc taataaacaa tgcattttgc tatatcattt
     1621 attttgttcg gctgtcaccc cttctttttt atctatttgt tctttttgct gttcggttgt
     1681 ttctgttccc gttttctttg gtattgtgtc aacaaccttt atttcaccag ctaacgaatc
     1741 aatagtggat ggattaaaat aacgtgaata atcatcatca tgatatcgtt tcaatgcctc
     1801 aactgaataa cctgctacaa taggagattt atttgcgatc aaatattcta tagttgccac
     1861 aatcaaatta aaattaaaat cctttacata atcacgtatt ataggttcag ctttctcaaa
     1921 ctctgcagtt aaaaggtatt ctgccacccg acgctcatcc ttaaaacgca ctcgtttttc
     1981 aacacgtttt ccatacgttt cctcgtcttt tgattcttgc tgcgttatat cgggtaattg
     2041 cacatcataa tactctttct gcatttcatc aattatttgc tgctcagttt ttgaaataaa
     2101 ccggacataa aatacatttc ccttttcacg ttcaatctca aaaagatgat tatatatctt
     2161 cttttttgtt tcatcgtttt gtgattcaaa gattgctaaa aagccatatc ggcgagctct
     2221 ccgtaccatc ttaattgctt caatatattt ataaatttta tttgagcaca gctttgggtt
     2281 ggaagcatct gcaactgtat gatgggtcct ggctacttcc ctgtctctgt acgccagatt
     2341 taaataaagg cgtgcttttt tattctttga ttttattacc tccggagaga tagaatctaa
     2401 aaattgtctg gtatcatcca gataatcttt aaccagcaaa gaatagagat taacctgttt
     2461 tttttgcgat tcataaacgt ttttaaacgc tctgttataa tctgattgca gataggcaac
     2521 ctctgcatta aaatgtatct cataaatatc tttaaactga tctttatatt tttgaccaaa
     2581 attagatatg ggaacatcca taaactcaat aaattcttta ttatcccgaa tagcactatc
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgatatgtat tatacctaat
     2761 acatatacca taagtgtaaa acttcactgt atccttataa atataacaca ccataccata
     2821 gcaaataagt caacaatttc acagtatatt tttactttta atccaatcct tttttatcag
     2881 atatactcaa acgcattttc tcaagtaacg aaaataattt tgggcttata tacacatcat
     2941 agtttactgg atcataaata tcttttattg atctggttaa agactgcata ttgttataac
     3001 aatgatgctg tatctcctgt agcgttggtt tatcatatac caatgttcca ttgtgaaaaa
     3061 taggtgcatg catgcatcgc gaaatcatat gcccatccag ggtaaactta ttacccttga
     3121 tatcataaac atctcccttt atgaccgtct cttcatcctg acatattaaa tcaaaccata
     3181 tggtattgtc agaatcatag tatctatata cattttttcg tgatggtatg gtcccttttg
     3241 aaatttcatc agagattttc attttgggta ttagcttacc atctttttca atggctgcca
     3301 ttttataaac tccggacaaa gccgattcac caaatcccgt cgcaagacgt gttccaacac
     3361 caaaaagatc gattggcgcc cccgccttta ataattcatc aatcttgtat tcatccagat
     3421 catttgaagc caatattttg atatactgca acccttcctc atccatcatt ttacgaacct
     3481 gtttacttaa atacgcaaga tcaccactat caagccttat tccaacaagg tgtttccctt
     3541 ttttagccag cttttttgct gctgctatcg cattttttat tcccgaacct aacgtatcat
     3601 aggtatcaac aagcaatata gaagtatccg gaaaaacttc agtataatga tcaaaagctt
     3661 catcttcatt atcataagcc ataacgaagc tatgtgcatg cgttccttta actggtatgc
     3721 caaacatttt gccggcaaga acatttgatg taccaacaca accccctata acagcagctc
     3781 tgctggcgct taaacccccg tcaggaccct gtgctcgtcg taatccaaat tcaataacat
     3841 ctctgccttt tgcagcataa catatccttg ccgattttgt tgcaatgagt gattgaaaat
     3901 ttatacaatt taacaatgcc gtttcaatga gctgtgcctg tcctaactca gcttcaactc
     3961 ttacaatagg ctcttccgga aaaacaatat ctccttcctg gaatgaataa atattaccgg
     4021 taaaactgaa agctttacag taatcaagaa aatcatcgct gaaaatattt aatgaatata
     4081 aataatctat actatcgttt gaaaatgaaa gattttgcag ataggcaata agctgttcca
     4141 atcctgcaaa aaccacatac cctgaaccaa aaggattctt tctaaaaaac atatcaaaaa
     4201 tggcatagcg atttattata ttcgttttga aataaacctg catcatcgtt agttcataca
     4261 ggtcagttaa taactccagt ccttgcattt cacacctctc ttaaggcaac ctgactatat
     4321 accattaata ttgatattgt aattaaaagt atacataaaa ttaatcaaat aaaaaatttt
     4381 ttatattgac agaattacta ttattaatcc ttaattaatt agttgttcat tctatatttt
     4441 tatcagttat ataaacaaat aattttttaa tgttttatag attacatata agaatgagta
     4501 tggattagtt tatggcattt gaacccgaga gcgataatct attcagtgat catttcataa
     4561 tcattaaagg ccgttttaaa gcattatcaa tgaacttata tggccttttc ataattatct
     4621 ttaatcagga taaacgtatc gatacctata atatcgctgt atcaacattt tctaatatat
     4681 atgaaaatga gcctcacctc ccatgggata aatatgagca gatgattcta gaacttaaat
     4741 ggaaaggtaa ttttaacgca aatcttacat catcaataat ggataatttt aaggaaatat
     4801 ctgccgaaga tattaagacc aatcaactat atgattatgc aaacagctac gactataata
     4861 gtcttgacac ctacctcttt gataccataa acagggttgt ccatgataaa aatcttgtgc
     4921 ttgaaaccag tattcaggag gttacagccg aagaatttgc ccaaaccaag gaaaaacgtt
     4981 ataacaaagc agaagaaaag tccacttcag aaaccgaact ggaagaaggc gctgttattt
     5041 tgaatattca gcctatttta gcacccgtga aaggcaaacc aatttatgaa cttaaaattg
     5101 gcgatagaat tatgacaaaa attattccca ataccgacag agccaactat ttcatagatc
     5161 ttctgggact gagagttgaa aatcatataa agccaattgc tactgaagta atagacatca
     5221 aatcagatgg gaaaagcgag ccccttgaga tactaaccag aattggtccc ggtatttatg
     5281 gtaaatgcat tgaagatgaa cgccaggtta aactaaggct gtacgatgct ctcattgatg
     5341 gtcctttaac acaaaaggtt atcccaaaac gaactcaacc atcgccaacc tcagttgcca
     5401 cctatgagga tgcgggtttt tcaaagctca cctatgttat tatctcatta tttgttttac
     5461 tgcttattat atttgttctt cttatttata taagctttta attgacaaat agagcatttc
     5521 tacagtaatt tcattcacac ctgaagccga ttaatacaac agcagtatat aatttgcaaa
     5581 aggaggcggc atatgtttta tctgcttcta agactgctgt atgaagaaat ggttgaagga
     5641 aagatatcac gggaagaata ttgtgaagca gtaatacgca tagcttcgct acacggattt
     5701 aagaggaatg gtatacaata atggacaatc gtgaatacat tatgaaaact gcacatgata
     5761 atgatgtgaa gtttatacgg ttatggttta ccgatatttt aggatttcta aaaagctttg
     5821 ccatcaccat caatgaactg gaagatgctc ttgaagaagg tgtgcgcttt gatggatcta
     5881 ccatacatgg atttgttcgt aaaactgaaa atgaaatgat agctttccct gacattgaaa
     5941 catttgaaat actaccatgg cggccaaaaa gtaactcagt agcacggatg ttttgttaca
     6001 tctataatgt tgatatgacc ccgtatgaat gtgatgtccg tcgcatttta tacaacaatc
     6061 taaaaaatgc tgcaaataga ggctatgtat tttatgtggg tccggagatt gaattcttct
     6121 tgttcagaaa tgctgaatct accgaatttc tggatagagg tggctatttt gacctgacac
     6181 ctcttgacat tgccagtgat atccgccgac agatggtatt aacacttgaa gaaatgggta
     6241 tcagtgtaaa atcatctcat catgaaggtg caccaagcca gcacgaaata gacctccgcc
     6301 acgatgatgc acttaccact gctgacaaca tcatgacatt taagatagca acaaaggaga
     6361 ttgcccagca gaacaatatg tacgcttcat tcatgcccaa gccactatcg cactacaatg
     6421 gttcagggat gcatatccat caatcactat ttaaagacga caaaaacata ttttatgatc
     6481 agcatgatga aaatctgctt tcacaaatgg caaaatgcta tattgctgga ttattgaagc
     6541 atgcacctga gttctttgtt ctttccaatc aatgggtaaa ttcatataaa cgattgcatt
     6601 atggatttga agcacctatt gacctttcat ggagcaccaa caacccatca tccctggtgc
     6661 gcattactac cagccgtacc gataaaccaa attcagtacg ggtagagctt cgaaatcctg
     6721 accctgcgtg caatccctat cttttgtttg cggctatttt agcctccggc ctggctggta
     6781 ttgacaacaa cgatgtttta cccaaaccac atgacctgac attgaaaaag cacgagcctg
     6841 ttgcacagcc attaccttca actatagaag aggcattaaa aaaatttgat aaaagccagc
     6901 ttatgcgaca gtcattgggt aataagctgg tagatttatt tgttgaaaat aaatattatg
     6961 agcttgatca gtacaaccgc catgttaccg actatgagct
//
LOCUS       DAOOJH010000112         6884 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_9147_length_6884_cov_10.330209, whole genome shotgun sequence.
ACCESSION   DAOOJH010000112 DAOOJH010000000
VERSION     DAOOJH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9147_length_6884_cov_10.330209"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PLJ75_11010"
     CDS             complement(<1..914)
                     /locus_tag="PLJ75_11010"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPD05782.1"
                     /translation="MHGHSIHDKSYKYLFSNPLIVKELLESFVAMEWIKEVDFSKAQP
                     VDKSYVNDDYKEYEADIIYKLMLKDAEIYLYLLIEFQSTVDNYMSFRMLTYIMELYRE
                     LIYKHKVKQLPVVFPILLYNGERKWTAATNIFQLINVPEQLQQCRPYIPQFQYYPIIE
                     NEILENELDAMMNVIATVFLLERGDKEIFLKTIDRIRNLMHIYGNENMIIRTLLVWLA
                     HYLETHGIIDNVKPIIESAFHPEEAYSMLSTTLQHLKEDLRKEGYTEGLQSGFQDGVQ
                     KGMQEGLQKGMQKGMQKGMQKGMQKGMQ"
     gene            1256..2257
                     /gene="ilvC"
                     /locus_tag="PLJ75_11015"
     CDS             1256..2257
                     /gene="ilvC"
                     /locus_tag="PLJ75_11015"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPD05783.1"
                     /translation="MAKMYYDNDADLSVISNKMIAVIGYGSQGHAQAQNLRDSGCKVI
                     VAELPGTPNYDIAVSHNFKPISAKEAAEKADIIQILAQDHVQAKLYENDVKPYLKKGK
                     TLVFSHGFNIHFGQIVPPSDVDVIMVAPKGPGHLVRSEYEKGGGVPCLIAIHNNATGK
                     AKEVALAYSKGIGATRAGVIETTFKEETETDLFGEQCVLCGGVSELVKAGFDTLVEAG
                     YQPEIAYFECMHELKLIVDLFYQGGINYMRYSVSDTAEYGDYMVGKRMVTEQTRKEMK
                     KVLDEIQSAQFARQWILENVAGRPQFNAIKKKEREHLIEKVGKELRKMMKWIDSKEV"
     gene            2460..4049
                     /locus_tag="PLJ75_11020"
     CDS             2460..4049
                     /locus_tag="PLJ75_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPD05784.1"
                     /translation="MSKKILLVYPEIPLTYWSFRYSLEFIGKKTVFPPLGLLTVAAML
                     PEDWEVELLDLNISSLDPTIIPDYDMVFISAMIVQQESFHHVTSMCKQYHVPVVAGGP
                     YPTSLWQSIPDVDYFVLNEAEVTLPMFLHDLQSGTLQHVYTSTQKPSLDKTPIPRFDI
                     IDLKPYSSVPLQFSRGCPFNCEFCDIISLFGRVPRTKSPQQFCAELDAVYATGFNESI
                     FIVDDNFIGNKAKVKELLKAIIEWQIAHAYPFSFQTEASVNLAHDDELMELMYKAGFD
                     MVFLGIETPVVSSLESVGKTQNTKENLLENVESIQQHGMEVSGGFIVGFDEDPDNVFD
                     LQIEFIQSSGIPMAMVGILDALPQTRLYHRLVEEGRLITESNGNNTHKLEPNFIPKMP
                     VQLLVDGYIKILKTIYSPKQYFERCLTLIKRLPGKRMVYRQFTLKEFKALILSLVKQG
                     FSSYGKDYFTFLLNTLKYRPKLFSEAIALAIKGYHLFKITDIMVNEYNKSLHKHGHDD
                     TIEKSLAATVPAGNEQLQTQL"
     gene            4076..4414
                     /locus_tag="PLJ75_11025"
     CDS             4076..4414
                     /locus_tag="PLJ75_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05785.1"
                     /translation="MSTPSTAVQILVTIIPIVGIVAGSVVLFFFLYFNHKQKILLIEK
                     GLYQKVSFDFDAFSLFTGFILLGAGASLTIFFLIKDGVSYALIGGLVPLGLGISFTCY
                     YFVKQKTTKQ"
     gene            4411..4968
                     /locus_tag="PLJ75_11030"
     CDS             4411..4968
                     /locus_tag="PLJ75_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPD05786.1"
                     /translation="MNNISDEHIIQQILKGNQELFKIIIQRYEQLVYSLGMRFFKNHD
                     DASDFTQEVFVKVYGNLYSFKGTASFKTWMMKVCYNHAVNKIAQMQNNNTTEYHDTIA
                     DTSEPLNIFMKNELGELLEQAINALPPAYKVCIDLYFYAGLPFKDISRITGYPVNTIK
                     SYVFRAKQHLRDALKGTIAEDYHEM"
     gene            4958..5410
                     /locus_tag="PLJ75_11035"
     CDS             4958..5410
                     /locus_tag="PLJ75_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05787.1"
                     /translation="MKCKKAIETFLMLDNNEFIPLLLKFHIAHCKQCYNEISQLQKAF
                     TSMQTSSSSPSIHLEDIIMSQVMLQKRYDYTISHLNWITTGLIIFIGIATISYSDALK
                     WMTYHFGIQLVLPLYLVLGCIIAAYISSYVATHLKKLQAIAHSIKSLL"
     gene            complement(5407..>6884)
                     /gene="polA"
                     /locus_tag="PLJ75_11040"
     CDS             complement(5407..>6884)
                     /gene="polA"
                     /locus_tag="PLJ75_11040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPD05788.1"
                     /translation="EGIYFDTMIASYLLNPGLRRHNIDDLAQEYLNYTKVSYEELVKD
                     GKKLQHITQVPMQKLSQYAIEDADIALRLYHILYQKLEQENLLDLFFTVEMPLVTVLA
                     YMEYYGVKIDLHHFEALSIELSNKLRDIEQKIYSLAGVPFNINSTRELSHILFEKLGL
                     KPVKKTKTGYSTDIQVLESLHGQHEIINYLIQYRTFNKLQTTYVDTLPKLVNPKTGRI
                     HTSFNQTVTATGRLSSSEPNLQNIPVRDEVGKKIRTGFIVDKGCSMLSADYSQIELRL
                     AAHLSKDPTMIEAFQKGIDIHAQTASSVFNVPLHEVTEQMRRQAKIINFATIYGVSPY
                     GLSQQADITVQQATEFIQKYFNAYPKIKEYIDTTIAFAQKHGYVQTLLGRKRSIPEIT
                     SSNQFRREGAERVAINTPIQGTSADMIKVAMVTIHTTFKEKQLKSKMILQVHDELVFE
                     VPNDEKELIEEIVVSAMKNAITLDVPVVVDTGWGNNWAEAH"
BASE COUNT         2190 a         1281 c         1204 g         2209 t
ORIGIN      
        1 ttttgcatac ctttttgcat acctttttgc ataccttttt gcataccttt ttgcatacct
       61 ttttgcaacc cttcttgcat ccccttctgg acaccatcct gaaaacctga ttgcaagcct
      121 tcagtataac cttcttttcg taaatcctcc ttcaaatgtt gtaatgtcgt tgataacata
      181 ctataagcct cctccggatg aaatgctgat tcaattatgg gttttacatt atcaataata
      241 ccatgagttt ctaaataatg cgccaaccat actaataaag ttcgtataat catattttca
      301 ttaccataaa tatgcataag gttgcgtatt ctgtcaattg tttttaagaa aatttcttta
      361 tcacctcttt caagcaaaaa aaccgtagct ataacattca tcattgcgtc taattcattt
      421 tccaatattt cattttcaat aattggataa tattgaaatt gcggtatata tggtctacat
      481 tgctgcagct gctcaggcac attaataagc tgaaatatat tagtagctgc cgtccattta
      541 cgttcaccat tatacaataa tatcggaaaa actactggca attgttttac tttgtgttta
      601 taaatcagtt cacgatataa ttccataata taagtcagca ttctgaacga catataatta
      661 tctaccgttg attgaaattc aataagcaga tataaataaa tttctgcatc tttcaacatc
      721 aatttatata tgatatcagc ttcatattct ttgtagtcat catttacata gcttttatca
      781 acaggctgag ctttgctgaa atcaacctct ttaatccact ccattgcaac aaatgattct
      841 aaaagttctt ttactattaa tggattagaa aaaagatatt tatacgattt atcatgaatt
      901 gaatgtccat gcataaataa taaccacctc ctgctataaa attttattca aatacataca
      961 atttccattg tatttctcta tgaaattaat acgctgaaac tggaaaaaat taaggggaaa
     1021 attaaaaaat atttttttat aattaaaaat ttttaaaatt tttctgttac caatagtgag
     1081 atatcacagg aaatcgataa ataacacttt tcaaacatta ccctgtttta agaagttaaa
     1141 aaaaaattaa atttttattg cataatacaa atacaataat agactatgct gcatcaataa
     1201 ctgcatgtaa gtatactgca tttactctat ataataatta aaacgaggta gcattatggc
     1261 aaagatgtac tatgataatg atgccgattt atcggtgatt tcaaacaaga tgattgctgt
     1321 aataggatat ggcagtcagg gtcatgctca ggcacaaaat ctgcgagata gtggctgtaa
     1381 agtaattgtt gcagaattac ccggaacacc aaattatgat attgcagttt cacataactt
     1441 taaaccaata agcgcaaaag aagcagctga aaaagctgat atcatccaga tattggctca
     1501 ggatcacgtt caggcaaagc tttacgaaaa tgatgtaaag ccgtatctta aaaaaggcaa
     1561 gacacttgtc ttttcacatg gatttaatat ccattttggt cagatagttc caccatcaga
     1621 tgttgatgtt ataatggtag caccaaaagg accgggacat ttagtacgta gcgaatatga
     1681 aaaaggcggc ggcgttccct gtttaattgc aatacataac aatgcaaccg gtaaagcaaa
     1741 agaagttgca ttggcttatt caaaggggat tggagctaca cgagcaggtg ttattgaaac
     1801 aacatttaaa gaagaaaccg aaaccgacct ctttggcgaa caatgcgtac tgtgcggagg
     1861 agtttcagag cttgttaaag caggttttga cacactggta gaagctggct accagcccga
     1921 gattgcttat tttgaatgta tgcacgaact taaactcatt gttgatttat tttatcaggg
     1981 cggcattaac tatatgcggt attctgtatc tgacactgcc gaatatggcg attacatggt
     2041 aggcaagcgt atggtcaccg aacaaacccg taaagagatg aaaaaggtac ttgatgaaat
     2101 tcaatcagct caatttgccc gtcaatggat tttagaaaac gtagcaggcc gtccacaatt
     2161 taatgcaata aagaaaaaag aacgtgaaca tctcattgag aaggttggca aagagttacg
     2221 aaagatgatg aaatggattg acagtaagga agtttaacaa taactatcga acagggcggt
     2281 gataaatcat cgctctgttc acatatcgaa atttttttgc aaccatcctg atagataacc
     2341 tgtctatata gtaaattcaa aactaaacag attaataaat cagcagttaa taatatgcat
     2401 ggttagtttt ccaaatatat aaactacctg tttggttcaa gacagcacag gaggttgtta
     2461 tgagcaaaaa aatattattg gtatatcctg aaatacccct tacctactgg agttttagat
     2521 actctttaga atttataggt aaaaaaacag ttttccctcc tcttggtttg ttaaccgttg
     2581 cagcaatgtt accagaggat tgggaggttg agctacttga cttaaatata tcttcacttg
     2641 atcccacaat aataccagac tacgatatgg tctttatatc agcaatgatt gtccaacagg
     2701 aatcatttca ccatgttaca tcgatgtgca aacaatacca tgtgcctgtt gtggctggtg
     2761 gcccctatcc tacttcttta tggcaatcta ttccggatgt tgattacttt gttttaaatg
     2821 aggcagaggt aacattgcca atgtttttac atgatctgca atctggaacg ctgcagcatg
     2881 tttatacttc aactcaaaag ccatcccttg ataaaacacc aataccacgt tttgatatta
     2941 ttgatcttaa accttattcc agcgttccat tacaattttc acgagggtgt ccatttaatt
     3001 gtgaattttg cgacatcatc tcattatttg gaagagttcc tcgtacaaaa agcccgcagc
     3061 aattttgcgc cgaattagat gcagtatacg caactggttt taacgaatca atatttattg
     3121 tagatgataa ttttattggc aataaagcaa aggttaagga actactcaaa gcaatcattg
     3181 agtggcagat tgctcatgca tacccatttt ccttccagac cgaagcaagc gttaatttag
     3241 cacacgatga cgaacttatg gagcttatgt ataaagcagg ttttgacatg gtttttctgg
     3301 gtattgaaac ccctgttgta tcatcacttg aaagcgtcgg taaaacgcag aacaccaaag
     3361 aaaatcttct tgaaaatgtt gaatcaattc agcaacatgg tatggaggta agcggaggct
     3421 ttattgttgg atttgatgaa gatcctgata atgtttttga tctacagatt gaatttattc
     3481 aatcatcggg gatacctatg gcaatggtag gaatactgga tgctctcccg caaacacggt
     3541 tatatcatcg tctggttgaa gaagggaggc ttattactga atcaaatggg aataatactc
     3601 acaagcttga gccaaacttt ataccaaaga tgccagtgca attgctcgtt gacggctata
     3661 taaaaatact gaaaacaatc tactcgccaa aacaatattt tgaacggtgc cttacattaa
     3721 ttaagcggct tccaggcaaa agaatggtat atcgccaatt tacattgaaa gagttcaagg
     3781 ctcttatact atcattagta aaacagggat tttcatcata cggaaaagat tattttacat
     3841 ttttgttgaa taccttgaaa tatcgcccaa aattattttc tgaagctata gcactggcta
     3901 tcaaaggata tcatttattt aaaatcactg atatcatggt aaacgaatac aataaatcac
     3961 ttcataaaca cggacatgat gatacaattg aaaaatcttt ggctgccaca gttcctgcag
     4021 gtaatgagca gctccaaaca caactttaat attaactgat agggggaact attgtatgtc
     4081 aacaccatca actgcagtac agattttagt aaccatcatt cctattgttg ggatagtggc
     4141 gggcagtgtt gtattatttt tcttccttta ctttaatcat aaacaaaaaa ttcttcttat
     4201 tgaaaagggt ttgtatcaaa aagtatcgtt tgattttgat gcatttagtc ttttcacggg
     4261 atttatactt ttaggtgcag gtgcaagttt aacaattttt tttcttatta aagatggtgt
     4321 ttcatatgca cttattggcg gattggtgcc attaggattg ggtatcagtt ttacctgtta
     4381 ttactttgta aagcagaaaa caaccaaaca atgaacaata taagcgatga gcatattatt
     4441 cagcaaattc ttaaaggcaa tcaggaatta tttaaaatca ttattcagcg ttatgagcag
     4501 cttgtttata gtttgggtat gcgttttttt aagaaccatg atgatgcatc tgattttacg
     4561 caggaggttt ttgtaaaggt gtatggtaat ctctattcat tcaaggggac ggcatccttt
     4621 aaaacatgga tgatgaaggt ttgctataac catgccgtca ataaaattgc acaaatgcaa
     4681 aacaacaata ccactgaata tcatgatacc atagctgata catccgaacc tttgaatata
     4741 ttcatgaaaa atgaactggg agagcttctt gagcaggcaa tcaatgccct gcccccggca
     4801 tacaaagtat gcattgatct ctatttttat gcgggattac cttttaaaga tatatcacgt
     4861 ataacaggat atcctgtcaa tacaatcaag tcgtacgttt tcagagcaaa acagcatctt
     4921 cgtgatgcat taaaaggaac aattgcggag gattaccatg aaatgtaaaa aagctattga
     4981 aacattttta atgctggata ataatgaatt catcccattg ctccttaaat ttcatatagc
     5041 acattgtaaa caatgctata acgaaattag tcaattacaa aaagccttta catcaatgca
     5101 aacttcaagc tcctcaccat ccattcattt agaggatata ataatgtctc aggttatgct
     5161 gcaaaaacga tatgattata ccatatccca tcttaactgg attacaacag gacttattat
     5221 tttcattggt attgccacaa tatcatacag cgatgcactc aaatggatga catatcattt
     5281 tggcattcag ctggtgctac ccttatatct tgtactggga tgtatcattg cagcgtatat
     5341 cagcagctac gtagcaacac atctaaaaaa attgcaagcg atagctcatt ccatcaaatc
     5401 cttgctttaa tgagcctctg cccaattatt accccagcct gtatctacca caaccggtac
     5461 atcaagcgta atggcgttct tcatggcgct tacaacaatt tcctcaatta attctttttc
     5521 atcattgggg acctcaaaca ccagctcatc gtgcacctgc agtatcattt tgctttttaa
     5581 ttgcttttct ttaaacgtag tgtgtattgt caccattgca accttaatca tatcagcaga
     5641 tgtcccctga atgggagtgt ttattgcaac acgctcagca ccctcacgtc taaactgatt
     5701 gctcgatgta atttcgggta tagagcgctt tctccccaga agtgtttgca catatccatg
     5761 tttttgtgca aatgcaatgg tggtatcaat atattcttta atctttgggt atgcattaaa
     5821 atatttttga ataaactccg ttgcctgttg cactgtaatg tcagcctgct gtgataatcc
     5881 atacggcgat actccatata tcgtagcaaa atttataatc tttgcctgcc tccgcatctg
     5941 ttcggttacc tcatgtaacg gcacattaaa aacagatgat gcagtctgtg catgaatatc
     6001 aatgcccttt tgaaatgcct cgatcatggt aggatctttt gacaggtgag cagcaagccg
     6061 tagttcaatc tgcgagtaat cagccgatag catgctgcat cctttatcaa caataaaacc
     6121 tgttcttatt tttttgccta cctcatcacg caccggaata ttttgcaaat taggttctga
     6181 tgatgaaagc ctgccggtag ccgttaccgt ctggttaaac gatgtgtgga ttcgtcccgt
     6241 ttttggatta acaagctttg ggagtgtatc aacgtaggtg gtttgtaatt tattgaatgt
     6301 tcggtactgt attaaataat taatgatctc atgctgtccg tgcaatgact ccagcacctg
     6361 tatatcggtt gaataccccg ttttagtctt cttaacaggt tttagcccta acttttcaaa
     6421 tagtatgtgc gatagctctc gcgttgaatt gatattaaat ggtacgccag ccagtgaata
     6481 aatcttctgc tctatgtccc gtaatttgtt tgatagctct atggacaatg cttcaaagtg
     6541 gtgtagatca attttaaccc cataatactc catataagca agcactgtaa caagcggcat
     6601 ctcaactgta aagaacagat ctagaagatt ttcctgttct aacttttggt acaggatgtg
     6661 atagagtcgc aacgctatat cggcatcttc aatagcatac tgcgacaatt tttgcatggg
     6721 cacctgcgtt atgtgctgga gtttttttcc atctttaact aattcctcat agcttacttt
     6781 ggtgtaattg agatattcct gcgctaaatc atctatattg tgtcgtcgca gtccaggatt
     6841 aagcaagtac gaagcaatca ttgtatcaaa ataaattcct tcaa
//
LOCUS       DAOOJH010000113         6346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10077_length_6346_cov_9.801144, whole genome shotgun sequence.
ACCESSION   DAOOJH010000113 DAOOJH010000000
VERSION     DAOOJH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10077_length_6346_cov_9.801144"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..2206
                     /locus_tag="PLJ75_11045"
     CDS             59..2206
                     /locus_tag="PLJ75_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05789.1"
                     /translation="MKTKFLFFMYILWLAACTSCINSNNETIDISSNWKLFLINNSND
                     ISNLDFAQSDYDDSQWHSITLPGKLSHKKAKQHLWLRKTFVIPDSLQKYDIAFVLGKI
                     WDVEQTYCNGIRIGSAGREYPNFHSEWNNFRYYYIPSSLVRYNQPNTIAIRVFSNQNA
                     EYNGTPLVTTLHNAKIINFYRTLIAENIPLSTSVMTLLLGVIALYYYLRNRDVLIIQF
                     AIISLLWCFSAMHFYLPDYGIFDFNDQDKLYYALTAGISVSVYIFFETITRSYNKLLR
                     IFIIITALIMAILSLSATVNDPVTGWRFQVIGGLGLVVQILWGYLIFKGIKQNQKEAK
                     ALCIPYLFMMLCVGHDSLAVSGFIYTNFFWINLGYPGLILGIGAILSQRTAIVADELL
                     KSKEYIENKNSELTQILNKVNASIKELYDIAQNVRETTRLLTDNMHEQNSNIEQTSAA
                     LEEFSSTVDLIASNALHQDTILQKNKELLFEYIKGLGHITDAAKSAVKLSYKSQGQTS
                     VTKEHLDDALKGIEKIKNYSDNIRQIAETINDIAEKTNLLSLNASIEAARAGEHGRGF
                     AVVADEIGKLAERSIEQSKSIQTILSQILADIETQTLLMGTVVYSNEDVERSVYMVNH
                     AIDTILDMCISQEELTKTIEENINTVLQGSTEITNATSEEKAAISEITATINQLLTIT
                     NTVYENTKILSETLSKITMQIDSLQSIAEIQSK"
     gene            2319..3650
                     /locus_tag="PLJ75_11050"
     CDS             2319..3650
                     /locus_tag="PLJ75_11050"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPD05790.1"
                     /translation="MCDTIVATQKATADGSVILAKNSDREANEAQLLKYIARRSWAND
                     TTVQCTYATIPQVKETNEIFISKPFWMWGCEMGANAHGLAIGNEAVFTKEPYEKTGLL
                     GMDLIRLSLERCNNAYDALMLTTELIEQYGQGGNAGMAHKLFYHNSFIFADSSCAYVL
                     ETANRHWVAIKVDGIRSISNGLTIEEEFTFASKGIEDYALKKGYLKKGDTFNFKKCFS
                     DTLYTYFSRCSDRQSRTTSCASKNSGTITPQSMFALLRDHGNDDMQLPPDKTSMGSVC
                     MHASLLPTRPSQSVAAFVAHLRDNIPAFWATGSSGTCTSVFMPFYLIGKDIDYFPAKE
                     TSTYSPESYWWMHEKIHRQSIFKWDYFQKTIAPSIISIENEFITKDNELFKKKNTTTQ
                     KYFDFSRLCVEKVLNINERWEKELKSAPYSNTKLSFTLFWNSQTKKAMIPV"
     gene            complement(3653..4318)
                     /locus_tag="PLJ75_11055"
     CDS             complement(3653..4318)
                     /locus_tag="PLJ75_11055"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05791.1"
                     /translation="MKICDGIFGYIWRGVFENNCNTFYLGEPLNMLIDPGLAKYVDMH
                     FTDMQKDGIDTKAIDYVIATHSHPDHFEGCAMFMGKGPQVVMHKDEIAFLHEVGPQFF
                     AMFGMQFPQFTFDIVLEEGEWKIGNEVLQVYKTPGHSPGSMCLYWASKKVLVCGDLIF
                     KESVGRTDFPGGDGTLLKESIQRMKELDIEYLLPGHMDIVVGKNNIKKNFELIEKYIF
                     PML"
     gene            complement(4322..>6346)
                     /gene="glyS"
                     /locus_tag="PLJ75_11060"
     CDS             complement(4322..>6346)
                     /gene="glyS"
                     /locus_tag="PLJ75_11060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPD05792.1"
                     /translation="AGYIPPAIQQIAALMSEGLKTHRIASGSCMVYATPRRIAVCVSD
                     MADKQSEDYEELKGPSVKAAYDAEGNPTKAFMGFLEGNKIKLEDTFIQNTSKGDYIYA
                     RRMVEAKPVTAIIPEMIEQIIMQVNFPKRMRWNVKRISFPRPIDYICVLFNDTVVMHS
                     IDGIPFDNKVRGHYIQNNCMIPLHYCREYGEVLEKHGVIVDHNKRKESIKQQLYSAAK
                     TLQGKLVEDEELLDTVTFLTESPYVVICSFDESFLTIPDIVLITEMKEHQKYFAIRDD
                     NGKLLPKFLVVSNNPPTEHIVKGNQRVIRARFTDARFFFEEDRKIPLADRVDMLKSIL
                     FHKELGSIYDKVVRVQKIAEVISQQLSFDDATMKKIDRAVLLAKTDLTTSLVFEFTSL
                     QGQIGKIYALLDGEDSEVADAIDFQYRPRFQGDILPSSIVSIVVSLAEKIDNIFGSFS
                     VGNIPKGSADPYALRRQSAAIVDMLVGNAIHLDLANVCKIIATQYNNGAQLVDAILDF
                     INGRAKTFLYEKGFAFDEVNACLAVGCYDYYELYLRAQSIHEFRRDKRFGDLLIAFKR
                     MFNIVNAFLKKNPGYTFSFNPDALVIEEEKNLYTFFKEKEDSIQEYIAHNKYKDLFLL
                     LIEGKEAIDTYFDKVMVMDSNPVVRDNRLGMLQSILTLFTAFIDFSQIQE"
BASE COUNT         2062 a         1308 c         1080 g         1896 t
ORIGIN      
        1 gttgacatat ctaaattatt cattatatac agttataaat acatcaggat atttttatat
       61 gaagactaaa tttttatttt tcatgtacat tttatggtta gcagcatgca caagctgcat
      121 aaactcaaat aacgaaacaa tagatataag cagcaactgg aaattatttc ttataaataa
      181 tagtaacgat atcagcaacc ttgactttgc ccaatctgat tacgatgaca gtcaatggca
      241 cagcattacc cttcccggaa aactatcgca caaaaaagca aaacagcacc tctggcttag
      301 aaaaacattt gttatccctg attcacttca aaagtatgac attgcatttg ttttaggtaa
      361 aatatgggat gttgagcaaa cgtattgtaa tggcatcagg attggctctg caggcaggga
      421 gtatcccaac ttccactccg aatggaataa ttttagatat tactatatcc cttcctcact
      481 tgtacgctac aatcaaccca ataccattgc catacgagtt tttagcaatc aaaatgcaga
      541 atataatggc acaccactcg tcactacatt gcataatgca aaaattatca atttttatcg
      601 cacactcatt gcagaaaata ttccgctatc cacaagtgtt atgactttac tgctgggtgt
      661 catagcacta tactactatc tcagaaacag ggatgtattg attatacaat ttgctattat
      721 ctcactgtta tggtgtttta gtgccatgca tttttatctt cccgattatg ggatatttga
      781 cttcaatgat caggataagt tatattacgc attaactgca gggatttctg tttcggtgta
      841 tatctttttt gaaacgataa cccggagcta caataaattg ctccgtatct ttataattat
      901 aacagcatta atcatggcaa tcctcagcct atcagcaact gtcaatgatc cggttacagg
      961 gtggcgcttt caggtaattg gtggtctggg cctggttgta caaatactat ggggctatct
     1021 tatttttaaa ggtataaagc aaaatcaaaa agaggcaaaa gctctttgca tcccctacct
     1081 ttttatgatg ctatgcgtag gacacgattc actggctgtt tccggtttta tatacacaaa
     1141 ctttttctgg ataaatttag gttatccagg actcatcctt gggattggtg ctatcttatc
     1201 acaacgaact gcaatagttg cggatgaact tctcaaatca aaagaataca tagaaaacaa
     1261 gaatagtgaa ctgactcaga ttttaaataa ggtcaatgca tccatcaaag aattgtatga
     1321 tattgctcaa aatgtcaggg aaacaaccag gctattaacc gacaacatgc acgagcaaaa
     1381 tagcaatatt gaacaaacat cagcagcact ggaggaattt tcatcaactg ttgacttgat
     1441 tgcatccaat gcattacacc aggatactat cttacaaaaa aacaaggagt tgctttttga
     1501 atatatcaaa ggacttgggc atatcactga tgcggcaaaa tcggctgtta aactgagcta
     1561 taaaagtcag ggacaaactt ctgttacaaa agagcattta gatgatgcac tcaaagggat
     1621 agaaaaaatt aaaaactact ctgataatat acgccagata gctgaaacca tcaatgacat
     1681 agctgagaaa acaaacctgt tatcattgaa tgcctcaata gaagcagccc gtgccggtga
     1741 acatggcagg ggttttgccg ttgttgccga tgaaataggg aaattagccg aaagatcaat
     1801 agagcaatca aaaagtattc aaacaatttt atcacaaata cttgccgata ttgaaaccca
     1861 gacccttctc atgggaacag ttgtctactc caatgaagat gttgagcgtt ctgtatatat
     1921 ggtaaatcat gctatcgata ccatccttga tatgtgcatt tcacaggaag agcttactaa
     1981 aacaattgaa gaaaatatca atacagtttt gcagggttcc actgaaatca ctaacgctac
     2041 cagtgaagaa aaagcagcca tcagcgaaat tacagcaaca ataaaccagc ttctaaccat
     2101 aacgaatact gtatatgaaa atacaaaaat actatcagaa actctatcaa agataaccat
     2161 gcaaattgat tcattgcaat ccattgctga aattcaatct aaataactat ttatgaaaac
     2221 tatttttatt tagtacttca tatagcaata tcaaaaatat tttgactttt ttattttatt
     2281 gctatacagt gttacttata taatgaaagg agattgttat gtgtgacacc attgttgcaa
     2341 cgcaaaaagc aactgctgat ggtagcgtaa tccttgccaa gaacagcgac cgcgaagcca
     2401 atgaagctca gttgttaaaa tatattgcca ggcgttcatg ggccaatgat acaactgttc
     2461 aatgtaccta tgccaccata cctcaggtta aggaaaccaa tgaaattttt atttcaaagc
     2521 cgttctggat gtggggttgc gagatgggag ctaatgctca tggattggcc attgggaatg
     2581 aagcggtatt tacaaaagaa ccatatgaaa aaacaggatt gctgggtatg gatcttataa
     2641 ggctttccct tgagcgttgc aacaatgcct atgatgcact gatgctaacc actgaactca
     2701 ttgagcaata tggacagggt ggcaatgcag gcatggctca caaattgttt taccataatt
     2761 cttttatttt tgccgatagt tcctgtgcat acgttcttga gactgcaaat aggcactggg
     2821 tagcaatcaa ggttgacggt atacgcagca tttcaaatgg attaactatt gaggaagaat
     2881 ttacctttgc atcaaaaggc atagaagact atgcattgaa aaaggggtat ttaaaaaaag
     2941 gtgatacttt taattttaag aaatgtttca gtgacacatt gtatacctat ttttcacgtt
     3001 gctctgacag acaatcaagg actacatcat gtgccagtaa aaatagcggt acaattacac
     3061 ctcaaagtat gtttgcattg ctcagggatc atgggaacga tgatatgcaa cttcctccag
     3121 ataaaacttc aatgggttct gtatgtatgc atgcttctct tctaccaacg cgcccaagtc
     3181 agtcggttgc tgcatttgtt gcacatctca gggacaatat ccccgctttt tgggcaaccg
     3241 gttcatcagg aacatgcacc agtgtcttta tgccatttta tctgattggc aaagatatcg
     3301 attatttccc cgcaaaagaa acaagtacct attcacccga aagttactgg tggatgcatg
     3361 aaaaaataca ccgccagtca atttttaaat gggattactt tcaaaaaact atcgcaccat
     3421 caataatatc tattgaaaat gaattcatca caaaagacaa tgagttgttt aaaaagaaga
     3481 atactaccac tcagaaatat tttgattttt ccagactatg cgttgaaaaa gttctaaata
     3541 tcaatgaacg atgggaaaaa gaattaaaaa gcgctcccta ttcaaataca aaattatcgt
     3601 ttacattatt ctggaattcg caaacaaaaa aggcaatgat cccagtatga tgctataaca
     3661 ttgggaatat atatttttca atcaattcaa aattcttttt aatattgttt ttccctacta
     3721 caatatccat gtgccccggc aacaggtatt cgatatcaag ctctttcatc cgctgtatac
     3781 tttctttgag caatgtgcca tccccaccgg ggaaatcagt tcttcctaca ctttctttaa
     3841 atatcaggtc gccacaaaca agcaccttct tagaagccca gtataaacac attgaacctg
     3901 gcgaatgtcc cggtgtctta taaacctgca atacctcatt gcctatcttc cactccccct
     3961 cttccagcac aatatcaaat gtaaattgtg gaaactgcat cccgaacatt gcaaagaatt
     4021 gaggaccaac ctcatgtaaa aacgctattt catccttatg cataacaacc tgggggccct
     4081 ttcccatgaa catcgcacat ccttcaaagt gatccggatg tgaatgcgta gctataacat
     4141 aatcaattgc tttcgtatca ataccgtcct tctgcatatc ggtaaaatgc atatcaacat
     4201 attttgccag cccggggtca atcagcatat taagaggttc gcctagataa aatgtattgc
     4261 agttattttc aaaaacacca cgccatatat atccaaatat accatcacat attttcatat
     4321 tctactcctg aatctgtgaa aaatcaatga aagctgtaaa caatgtaagt attgactgta
     4381 acattcccag cctattatcg cggacaacag gattactgtc cataaccata accttatcaa
     4441 aataggtatc aatagcctct ttgccttcta tgaggagcaa aaataggtct ttgtacttat
     4501 tgtgtgctat gtattcctga atgctatcct ctttttcttt aaaaaacgta tataaattct
     4561 tttcttcttc aatgacaagc gcatcaggat taaatgaaaa tgtgtatcca gggttctttt
     4621 ttaaaaatgc attgacaata ttaaacatac gtttaaatgc tatgagaaga tccccaaacc
     4681 gtttatccct tctgaattca tgtatgctct gtgctctcag atatagctca tagtaatcat
     4741 aacatcctac tgccaaacat gcattgacct catcaaaggc aaaacccttt tcatataaaa
     4801 atgtttttgc cctcccatta ataaaatcaa gaatcgcatc aaccaattgg gcaccattat
     4861 tatactgtgt ggctattatt ttacaaacat ttgcaagatc cagatggata gcattcccaa
     4921 caagcatatc aacaattgct gctgactgac gtcgcagtgc ataaggatct gcagagcctt
     4981 ttggtatatt accaacggaa aatgaaccaa aaatattatc aattttttca gctaacgata
     5041 caacaatact cacaatactt gaaggtagta tatcaccctg aaatcgtggc cggtattgga
     5101 aatcaatagc atcggcaacc tcactatcct cgccatcaag aagcgcataa attttaccaa
     5161 tttgcccctg taaggacgta aactcaaaga caagggacgt cgtaaggtcg gtctttgcca
     5221 atagcacagc acgatctatc tttttcatag tagcatcatc gaaggatagt tgctgactta
     5281 taacctcagc tatcttctgc acccttacta ctttatcata gatagagccc agctctttat
     5341 ggaaaaggat agactttaac atatcaacac gatcagctaa tgggatcttt ctatcctctt
     5401 caaagaaaaa tcgtgcatcc gtgaagcgtg ccctgataac acgctgattt cctttcacaa
     5461 tatgctctgt tggtggatta tttgatacaa ctaaaaattt tggcaatagt ttgccgttat
     5521 catcacggat ggcaaaatac ttctggtgct ccttcatctc agtaataagg actatatcag
     5581 gaatagttaa gaacgactca tcaaaactac agataactac ataggggctt tctgttaaaa
     5641 aggtaacagt gtccaggagt tcttcatctt ccaccagttt gccctgcaag gttttagcag
     5701 cactatacaa ttgttgttta atactctcct tccgcttatt gtgatcaact atcaccccat
     5761 gtttttctaa aacttcacca tactcccggc aataatgcag gggaatcata caattgttct
     5821 ggatataatg ccctctgact ttattatcaa acggtattcc atcaattgaa tgcattacaa
     5881 ccgtatcatt gaagaggaca cagatataat caataggtcg gggaaaacta atccgtttaa
     5941 cattccagcg catacgtttg ggaaaattta cctgcataat gatttgctca atcatctccg
     6001 gaataattgc tgttaccggc tttgcttcta ccatgcgtct ggcatagata taatcaccct
     6061 tacttgtatt ctgaataaat gtatcttcca attttatttt gttcccttct aaaaacccca
     6121 tgaatgcctt tgtggggttt ccctctgcgt cataggcagc cttaaccgat ggacctttta
     6181 attcttcata atcttccgat tgcttatcag ccatatcgct gacacatact gcaatgcgcc
     6241 gtggcgttgc ataaaccata cacgagccag atgcaatcct gtgcgttttc aaaccttcac
     6301 tcataagtgc ggctatttgc tgtatagcag gaggaatata tcctgc
//
LOCUS       DAOOJH010000114         6324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10121_length_6324_cov_9.967618, whole genome shotgun sequence.
ACCESSION   DAOOJH010000114 DAOOJH010000000
VERSION     DAOOJH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10121_length_6324_cov_9.967618"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="PLJ75_11065"
     CDS             complement(<1..1087)
                     /locus_tag="PLJ75_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPD05793.1"
                     /translation="MEVINFSLGRVPSTMFFAGAIAQLPFAVEKFGKKILLVTGGSSL
                     KKSGRLDAIERLLKSKNIYYAIVTVHGEPSPQLVDAVVKQFADKEINAVVSIGGGSVI
                     DAGKAISAMLPLNESVYDYLEVVGTKLHGGVKIPFIAIPTTAGTGSEATKNAVLSTTG
                     TSGFKRSLRHNNFVPDIAIIDPELMIGCPFNITAASGLDALTQLIESYVSTKANPFTD
                     ALSLSGIEKVQQSLLPLCIDKNDSIPLRAGMAYAAYVSGITLAHAGLGIVHGLASPLG
                     ALFGIPHGVACGTLLAAAARITIIKLKSAGDTKYLQKYAKVGELLAPEKGGGDTAQKT
                     HNCIHTLEEWTETLAMPRLSMYGVREQD"
     gene            complement(1078..1389)
                     /locus_tag="PLJ75_11070"
     CDS             complement(1078..1389)
                     /locus_tag="PLJ75_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HPD05794.1"
                     /translation="MIVNVVIVHVKKEHINDFITATINNHKNSLQEPGNLRFDVLQSQ
                     DDPTQFILYEAYRTQQDANAHKNTPHYAVWKDTVALWMEEPRRGTAYTVIAPLEESLW
                     K"
     gene            1604..2296
                     /locus_tag="PLJ75_11075"
     CDS             1604..2296
                     /locus_tag="PLJ75_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPD05795.1"
                     /translation="MNILIFGPNGSGKGTQGAVVQKKFNISHIESGVIFRENIKKGTE
                     LGLKAKTYIDRGDLVPDDITIPMILNRLREPDCKNGWLLDGFPRNVEQAKAIDKALKE
                     AGMALDYVIEIVLDRESAKKRIMGRRLCENDNNHPNNIYIDAIKPAEKDGQIVCRVCG
                     GKLSTRADDQDEAAINKRHDIYYDTKTGTLAAVQYFKDISKQSGGKPKIIEVDGTPGV
                     KEVSEALLKKLG"
     gene            complement(2561..2974)
                     /locus_tag="PLJ75_11080"
     CDS             complement(2561..2974)
                     /locus_tag="PLJ75_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05796.1"
                     /translation="MQKELRKIIIFMIIMISLVVIITYHYGKVTYNHEKELTAPVYQF
                     ISKCDEEYIKKNNKHMADAIHTSVVTLAYNIFYNGKHKGVIEKDTSVFKRFQPELSPE
                     AKKASDASRKLLLKNKMQKELSNVCPDTVNTFMNE"
     gene            3248..3808
                     /locus_tag="PLJ75_11085"
     CDS             3248..3808
                     /locus_tag="PLJ75_11085"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPD05797.1"
                     /translation="MGEIKVGEKIKEFREKKNMSLHELADKTGFSSAFISQIENHLIS
                     PPLGAIIKIAHALSTEMGRFFGQTTSAPFTLVRSNERELTSRVASKEGVRYGYTYESL
                     APEKINRHMEPFIVTLEPVQGGQPYSHEGEEFIYVLEGEVKIQLADYSDILYPGDCIY
                     YDSIVPHRVDTATENSAKILAVLYSS"
     gene            3934..5523
                     /locus_tag="PLJ75_11090"
     CDS             3934..5523
                     /locus_tag="PLJ75_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPD05798.1"
                     /translation="MPGIIGQIIAGVVIGPTFIGNIMPDFFSWLFPSSGYLKGVVFLG
                     LIFIMLKIGIEVELSSIFKQGKSIVVISFFSIIIPFLVGFFASWYCPVVTGGSTRALS
                     VSLFFSIAIAITALPVIAKILLDVKIFHSDLGMVILISAMINDIIGWILFSSLIKSIE
                     SEVIAFSTIVYSLLVTITFAIFIVTIFRYVLNEILPFIQAKMEWPGSVITITLVLSMF
                     IASFTESIGMHAFFGAFLAGIAIGDSSHIKSHTKDILNQFIDNFFSPLFFVSIGLQIN
                     LLTHFSLLQSLSLIIMIFISNIIGSFIAGTIVKNTFRDSLAIGIGMSTSGTMGIIVGL
                     FALQYGIINQTTYTSIVIMAVVTSLAAGPLLKLILKPPKAITLIDLIDNNFIPDCKSN
                     NRYELIHELANEVQKRWNIDAEVVAQKIIEREMTMSTGIGNAIAVPHARLTYCKKPLI
                     LCTTSKQGIDFDAPDGKPAHIIFLIITPHEDHDSQIYILAEISRIFSNASVRDKVMNA
                     TNNNEFIAILKNTYHMLSLRE"
     gene            5525..>6324
                     /locus_tag="PLJ75_11095"
     CDS             5525..>6324
                     /locus_tag="PLJ75_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05799.1"
                     /translation="MELSDIIKNDDDRLFIITPECFIVFTGDSIEDEKPFIRIGNSLS
                     LTPRIIPLIENIIITDLITGNPAHEQFNIDPRYLSSNRYIGSKLIVKRYLEYQKLFGL
                     DLHNATIVDIEEDIPELSKEQIISDRETFLGIFYTDNNFKILHNQKIIFDLKEIHQKY
                     PGDVLVHNKLSETSSSIRFSGCGFAIAPDSIFFYKNNAFSTIEFPASSYYTAFSSLQI
                     DPANIRDAIITTANTIPLIPIVKWKNALSGRLRIFYDNEEEVKLLKKL"
BASE COUNT         1989 a         1146 c         1127 g         2062 t
ORIGIN      
        1 aatcctgttc ccttactcca tacattgaaa ggcgtggcat ggctaatgtt tccgtccatt
       61 cttccagcgt atgaatacaa ttatgtgttt tttgtgctgt atcgcctcca cctttttcag
      121 gtgcaagcag ttctcccacc tttgcatact tttgtaaata ttttgtatca ccagcacttt
      181 ttaattttat tatcgttatc ctggctgctg cagcaagcaa agttccgcat gcaacaccat
      241 ggggaatgcc aaataaagca cccaatggcg aagccagccc gtgcacaata ccaagccctg
      301 catgggcaag cgtaatcccg gaaacatacg cagcatacgc catgccagcc cgtaacggta
      361 tagaatcatt tttatcaata cacaatggta aaagactttg ctgcactttt tctattccac
      421 tgagtgacag tgcatcggta aaaggatttg cctttgttga aacatatgac tcaattaatt
      481 gagtaagagc atcaaggcca cttgcagcgg ttatattaaa agggcaccca atcatgagtt
      541 caggatcaat aatagcaata tcagggacaa agttattatg gcgtaatgag cgcttaaaac
      601 cgctggtgcc ggtagtactg agtacagcat ttttagttgc ttcacttcct gtacccgcag
      661 tagtggggat tgctataaat ggaattttaa caccaccatg gagctttgta cccacaacct
      721 ccagatagtc ataaacactt tcattcaatg gcaacatggc tgagattgcc ttgcctgcat
      781 cgattacact gcccccgcca attgaaacga ctgcattaat ttctttatct gcaaattgct
      841 ttaccactgc atcaacaagt tgcggcgacg gttccccatg tacagtaact atcgcataat
      901 aaatattttt gctttttaat aagcgttcaa tagcatccaa tcgtcctgat tttttaagcg
      961 atgagccacc ggttaccagc agtatttttt ttccaaattt ttcaacagca aatggcagtt
     1021 gtgcgatagc tcccgcaaaa aacatggtag aaggcacccg tcccaatgaa aaattgatta
     1081 cttccataat gattcctcaa gtggtgcaat gacggtatac gctgtacctc ttcgaggctc
     1141 ttccatccag agggcgacag tatccttcca cactgcataa tgcggtgtgt ttttatgagc
     1201 attagcatcc tgctgcgtgc ggtacgcttc atacagaata aattgcgtgg ggtcatcctg
     1261 tgactgcaac acatcaaaac gcaggttccc tggctcctgc agtgagtttt tatgattgtt
     1321 gatagttgcc gttataaagt cattgatatg ttcttttttt acatggacaa taactacatt
     1381 tactatcata ccttccccct gtaattgaat tattaaagca atataacgct gttgtcagga
     1441 tgtattactt ttatgaaaga atcaagtata tattaatggt atgagcacta tcaaacaaat
     1501 aaaaacttac tttacattta tttatagttt tttactgttg ctacgaaatg atttttagct
     1561 gaagattcag catatatagt atactaatcc aagaggtgtt accatgaata ttttaatatt
     1621 tggtcctaac ggcagtggta aagggactca gggcgcagtt gtacaaaaaa aatttaacat
     1681 ttcccatatt gagtcaggtg ttatttttag agaaaacata aaaaaaggca ccgagctggg
     1741 acttaaagca aaaacataca ttgaccgcgg tgatctggtc cctgatgata tcactatccc
     1801 aatgatttta aacaggctca gagaaccaga ttgtaaaaac ggctggcttc ttgatggatt
     1861 tccacgaaat gttgaacagg caaaagccat tgataaagcg ttaaaagaag ctggtatggc
     1921 acttgattat gttatagaaa tagtgcttga tagagaaagt gccaaaaaac gaataatggg
     1981 gagaaggctc tgcgaaaatg acaataatca tcctaacaat atctatatag acgcaataaa
     2041 acccgctgaa aaagatggcc agatagtatg ccgagtatgt ggcggcaagc tgtccacccg
     2101 tgctgatgat caggatgaag ctgctatcaa taagcgtcat gatatctatt atgacacaaa
     2161 gaccggaacg ttagcggctg tacaatattt taaagatatt tcaaaacaga gtggtggaaa
     2221 gccaaaaatt atagaagtag acggaacgcc cggtgttaag gaagtaagtg aagcattgct
     2281 taaaaagttg ggatagcaac aatacgtata cagggggttg acactctata attgcaaccc
     2341 cttgtgttta tattaatgtt gtatgaatgc aaatgaaaaa atatggtttc ctgtgagatt
     2401 ttatttcctt cactataaaa atttgatact gcattttctg taaaatgaaa gtcattaaaa
     2461 aatagaatta ttaacctcat cccctgccct atctccttag taaagtgaag ggagttttta
     2521 caccattgtc atgctgaggg agcgttagct accagagaat ctattcgttc ataaatgtgt
     2581 taacagtgtc aggacaaaca ttgcttaatt ctttttgcat tttattttta agtaaaagtt
     2641 ttctggaagc atcactcgct ttttttgcct ctggcgacag ctctggttga aaccttttaa
     2701 atacggatgt atctttttcg attacacctt tgtgtttacc attataaaaa atattatatg
     2761 ccagtgttac aacagatgta tgtattgcat ctgccatatg tttgttattt tttttgatat
     2821 attcttcatc acacttgctt ataaattgat ataccggtgc tgttaattct ttttcatgat
     2881 tatatgtaac cttcccatag tgataggtaa tgataaccac caatgaaatc attataatca
     2941 taaaaataat aatttttcgt aattcttttt gcatatatac caacctactg tgctcatgca
     3001 actatttaat gtattccaac gtcaatctgt aatgcaggta ttataaatta tagtatgaaa
     3061 agtaaagaca tcaaaattat tgtatggcta tccgggcaat tgaatatatt gcaaagcgat
     3121 agttattaaa atataaaagt attcatgaat attgttaaaa caatttgact tttttaatca
     3181 aaataataca ctttggttga aatcagcata tcatgatgtc aattataata cagtaaggga
     3241 gttaattatg ggtgaaatta aggttgggga aaaaataaaa gaattccgtg aaaaaaagaa
     3301 tatgtcattg catgaattag cagacaaaac aggcttttca tcagctttta tttcacagat
     3361 tgaaaatcat ctcatctcgc cgccattggg tgctattatc aaaatagcac atgccctatc
     3421 aacagaaatg ggaagatttt ttggtcagac taccagtgca ccatttacac ttgtccgttc
     3481 aaatgaacga gaactgacat cacgagttgc ctctaaggaa ggcgtccgat atggatatac
     3541 ctatgagtca ttggcacccg aaaaaattaa tcgccacatg gagccattta tagtaacgct
     3601 tgaaccagta cagggcggac agccatatag ccatgaaggt gaagaattta tatatgtttt
     3661 agaaggtgaa gttaagatac aattagccga ttactctgat atcctttacc cgggtgactg
     3721 catctattat gattcaatag ttcctcacag ggtagataca gcaacagaaa attcagcaaa
     3781 aatattagct gtactataca gctcctaact actggctata aacgggtatt gataatgttg
     3841 ctttcatcgt atgaagcttt gagcctttta ctttcactca gcactatgtt gctggcttca
     3901 aaaatcttta gtgagatatt tactaaatta aggatgcctg gcattattgg tcaaattatt
     3961 gcaggtgttg ttataggtcc aacatttatt ggcaacatta tgcctgattt tttttcatgg
     4021 ctttttcctt catctgggta tctcaaaggc gtggtgtttt tagggcttat atttattatg
     4081 ttaaaaattg ggattgaggt tgagctatct tccattttta aacaaggcaa atcaatagta
     4141 gttattagtt ttttttcaat tataatacct tttttagttg gtttttttgc atcatggtac
     4201 tgtccagtag ttacaggtgg atcaacaagg gcattaagcg tatcgttatt tttttcaatt
     4261 gctatagcaa ttacagcatt acctgtaatt gcaaaaattc ttcttgatgt aaaaatattc
     4321 cattcagatc ttggcatggt aatcctgatt tctgcaatga taaatgatat cattggatgg
     4381 atactatttt catcattaat caaatctatt gaatcagaag ttatagcatt cagcacaata
     4441 gtatattctt tactagttac tatcactttt gcaatattta ttgtaacaat attcaggtat
     4501 gttttaaatg aaattttgcc ttttattcag gctaaaatgg aatggccggg aagcgttata
     4561 acaataactc ttgttttatc tatgtttatt gcttctttta cagaatcaat agggatgcat
     4621 gctttttttg gtgcatttct tgcaggtatt gcaataggcg attcttctca tatcaagtcc
     4681 catactaaag acattttaaa tcaattcatt gataattttt tttctccctt attttttgtt
     4741 tctataggct tgcagataaa tcttttaaca catttttctt tgctacaatc gttatctctc
     4801 attatcatga tatttatttc aaatatcatt ggtagtttta ttgctggtac tattgtaaaa
     4861 aatactttta gggattcgct tgctattggt ataggcatga gcaccagcgg aaccatgggg
     4921 ataatcgtgg gattatttgc tttacagtat ggtatcatta atcaaacaac ttatacttct
     4981 atagtcatta tggctgttgt cacatcactt gcagccgggc ctttattaaa attaatctta
     5041 aagcctccaa aggctattac attaattgat cttatagata acaacttcat ccccgactgt
     5101 aaatctaata accggtatga acttatacat gaacttgcaa acgaagtgca aaaaaggtgg
     5161 aatattgatg ctgaagttgt tgcacaaaaa attattgaac gtgaaatgac tatgagtaca
     5221 ggtataggca atgccattgc tgtacctcat gcacgtttaa cttattgcaa aaagccgctg
     5281 atattatgta caaccagtaa acagggtata gattttgatg cacctgatgg gaaaccagca
     5341 catattattt ttttaataat taccccccat gaggatcatg attctcagat ttatattctt
     5401 gcagagattt caaggatatt ttctaatgct tcagtgcggg ataaagtaat gaatgcaacc
     5461 aataataatg aattcatagc tattttaaaa aatacttatc atatgctttc attgagggaa
     5521 taacatggaa cttagcgata taataaaaaa cgatgacgac aggcttttca ttattacacc
     5581 agaatgtttt attgtattta ccggcgattc aattgaagat gaaaagccat ttatacgcat
     5641 tggcaacagc ctgtcattaa cgccacgtat aatccctctg attgaaaata ttattataac
     5701 tgatcttata acaggcaacc cggcacatga gcaatttaat attgacccgc gttacctttc
     5761 atcaaatcgc tatattggca gtaaattaat tgtaaaacgc tatcttgaat atcaaaaact
     5821 ttttggactt gatcttcaca atgcaacaat agttgatatt gaagaagata taccggagct
     5881 ttccaaagag cagattatct ctgaccgtga aaccttttta ggcatctttt atacggataa
     5941 caattttaag attcttcata atcaaaagat aatatttgat ctaaaggaaa tacatcaaaa
     6001 atatcccggg gatgtactgg ttcacaataa gctgtctgaa acgtccagta gcattcgctt
     6061 ctcgggttgt ggctttgcga tagctcccga tagcattttt ttctataaaa acaatgcatt
     6121 cagtaccata gagttcccgg cttcttccta ttacactgca ttttccagtt tacagattga
     6181 tcctgcaaac attcgcgatg ctattattac aactgcaaat accatcccat taattcccat
     6241 tgtgaaatgg aaaaatgcac tttccggtcg attacgaatc ttttatgaca acgaagaaga
     6301 ggttaagctt ttgaaaaagc tctt
//
LOCUS       DAOOJH010000115         6269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10220_length_6269_cov_9.718700, whole genome shotgun sequence.
ACCESSION   DAOOJH010000115 DAOOJH010000000
VERSION     DAOOJH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_6269_cov_9.718700"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLJ75_11100"
     CDS             <1..429
                     /locus_tag="PLJ75_11100"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPD05800.1"
                     /translation="VEALVKASIPVMGHLGLTPQSVHKLGGYSVQGKDEEQRKTILED
                     ALLLQEAGAFAIVLEMVPEPLAKEITQRLAIPTIGIGAGRYCDGQVLVIHDLLGLNKG
                     FNPKFLKRYADLYTISQQAIQNYHKEVKEHTFPAEENVFK"
     gene            complement(430..1350)
                     /locus_tag="PLJ75_11105"
     CDS             complement(430..1350)
                     /locus_tag="PLJ75_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05801.1"
                     /translation="MSMISKQITIVCGSLIIVSMTVCSWAQDKNRVTFLYTNSLNGII
                     DACQCAADPKGGLVKRGYAVAQLKTKYPGAVLLESGDFCSYYPDTMLTKYIMKAYRHI
                     GYTAVGIGDQEFAAGVNTFHQYINELPFVSANLYYSIGGRWLSPKKNITVTVGTMKIG
                     ITAIIDNDAFKYYPNDIITKIKVTDITEALKKEIAAMEKMDFIVLLSHCGFEKDKELA
                     AQFPQIGLIVGGHSQTLIKKPVKIGNTFIVQAGANGARIGVLDAVITNKYIASYKHTF
                     IHPRYEDPDDEAIAKMIKEYNDEIGKQFPR"
     gene            complement(1583..>2029)
                     /locus_tag="PLJ75_11110"
     CDS             complement(1583..>2029)
                     /locus_tag="PLJ75_11110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPD05802.1"
                     /translation="PMEIVTNDDIKNLKIKRGSLNEDERHEIESHVIHTYNFVSTIPW
                     PPEFSQIPEIAIKHHEKLDGTGYPYGLKGDAIPLQARIMALADIFDALIATDRPYKPP
                     ITFNQALKIIEEEACNNKLDKELVALFINKKIYDQFDRDAYRIKAQ"
     assembly_gap    2030..2129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2130..3442)
                     /locus_tag="PLJ75_11115"
     CDS             complement(<2130..3442)
                     /locus_tag="PLJ75_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPD05803.1"
                     /translation="MESLPVHTCFIIDASLSKVISTPETIDATIHYVSHPYNELPDIL
                     HLLIANVNSCYALVCIIPSDCYESIGTVLNTIDISGKNVYINYVCVGNLHQRFDAIEG
                     LMAFYSDSITMDEFLYIAQRSQYNAHQHFEIKSLTRHYLHELEDTERDFEALINIGKA
                     LSIEKNPDKLLRLILHLSKTITGADAGSIYIVEEKEGNKLLRFKHSHTFSKELPYEEF
                     SMPMDKCSIAGYVAVTQQVLNIPDVYQLGVDDPVSFNSSFDKAHGYRSKSMLVVPMVN
                     HINQTIGVIQLINSKEDFNNDYKGNEAYSVKLISKDDFETKVVPFQKRYESLMEAVAS
                     QAAIAIENNRMIRQIQNQFEEFVRASVTAIESRDVATSGHSFRVADMCLKIANAINED
                     HDEAFASVTFTETQLKELEYAALLHDFGKVYIDTAIFLKSHKLYP"
     gene            complement(3513..4922)
                     /locus_tag="PLJ75_11120"
     CDS             complement(3513..4922)
                     /locus_tag="PLJ75_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPD05804.1"
                     /translation="MKKSLFKKGAYIYIEGDEDADTVYIVDKGVVQLSRYNKNAPIYK
                     NILKGGEFFGVTASLCNKPRMESAIALTDSIITSLTRQEFINLLSKTPQIAIKVLNYF
                     SDELRAYNEMMIALKDKEVELETPEEALYNHINYFYKNNSYAYALYCCKRYGELYPGG
                     MFATAVKSIMEKIKGKVKGSIVPVNEPPYKIFMDQQMIFCEDEPGNEMYIIKEGKVKI
                     IKLSSESEIILSVLKKGDVFGEMALISDKLRNAAAISWGKTILLPIKKESLVAVMKQM
                     PAITQLIFKELSHRIWFTYIQLESQLYDKPITRIYALLENKLIEKNVSLKSNEPVKLN
                     FGIDELLRMLGYISSKMEKSTNILLGDSNLEFNIGEIIVQKPSQLSAKAKYFKSRDHM
                     AIIESEEEKKEIEKRQGMEIVTEPAVNEKVSTIEEMHVTATTMNEADTFLQFEEKTDE
                     KSDHYKKEELHVISEEIDI"
     gene            complement(4929..5477)
                     /locus_tag="PLJ75_11125"
     CDS             complement(4929..5477)
                     /locus_tag="PLJ75_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPD05805.1"
                     /translation="MKIIVPLAPGCEEIEAITIIDVLRRANLNVVSAGLTDSLITGSH
                     GIAIKPDILLHKANPVDYNCIVLPGGMPGSENLKKDDDVIKFIKHIFSKGGYVAAICA
                     APIVLGSAQILYGKKATCFPGLEGELIGATLINEPVVVDGTIITAKGPACAIPFALTL
                     AGLFAGKDVQDTLKNAMQVYWM"
     gene            complement(5800..>6269)
                     /gene="pth"
                     /locus_tag="PLJ75_11130"
     CDS             complement(5800..>6269)
                     /gene="pth"
                     /locus_tag="PLJ75_11130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD05806.1"
                     /translation="GILVGVAIAHLYSITVRKKEHDALTGSGIIDGNECIFMIPQTYM
                     NNSGIAVQKVLSYYKELPDNLIVLHDDIELTFGNVALKFGGGHKGHNGIRSIQQHVGT
                     PDFYRIRIGVGRPSDAIPVADYVLSDFTQEEKSYCEDIIHKTNSILIQCLHQK"
BASE COUNT         1840 a         1289 c         1036 g         2004 t
ORIGIN      
        1 gtggaagcgc ttgtcaaagc ttctatcccc gtcatgggac atcttggttt aacaccacaa
       61 tcagttcata aattgggcgg ttactccgtt cagggcaagg atgaagagca acgaaaaacc
      121 atactggagg atgcgctgct gttacaggaa gcaggagcat ttgccattgt actggagatg
      181 gtgcccgaac cattagcaaa agagataacc caaagacttg ctatccccac tattgggata
      241 ggggcaggac gctactgtga tgggcaggtg ctggttatcc atgatctttt agggttaaat
      301 aagggtttca atccaaaatt tcttaaacga tatgctgatc tctatactat atcacaacag
      361 gcaatccaaa attatcacaa agaagttaaa gagcatacct tccctgcaga ggaaaatgtg
      421 ttcaagtaac tatcgtggaa actgctttcc aatctcatca ttatattcct tgatcatttt
      481 tgcaattgct tcatcatcgg ggtcttcata tcgcggatgg ataaatgtat gcttatacga
      541 agcaatatat ttattagtta ttacagcatc aagcacgcca atacgcgccc catttgctcc
      601 tgcctgcact ataaacgtat tgccaatctt tacaggcttt tttataagtg tttgtgaatg
      661 tccaccaaca ataagtccaa tctggggaaa ctgcgctgcc agctctttat ccttttcaaa
      721 accgcaatgc gaaagcagca caataaaatc cattttttcc atggcggcaa tctctttttt
      781 caatgcttcg gttatatcgg taaccttaat cttggtgata atatcattgg ggtagtactt
      841 aaaggcgtcg ttatcaataa tggcggtaat acctattttc atggtaccga cagttactgt
      901 tatatttttt tttggcgaca accatctgcc accgatagaa tagtacaggt tggcactcac
      961 aaacggcagc tcattgatgt actgatggaa tgtgttaacc cctgcagcaa actcctgatc
     1021 gccaatcccc acagccgtat agccaatatg ccggtaggct ttcataatat attttgtaag
     1081 catggtgtca ggatagtaac tacagaaatc tccactttca aggagtactg cgccaggata
     1141 ttttgttttt aattgggcaa cagcataccc gcgcttaaca agccctccct taggatcggc
     1201 agcgcactga caggcatcga taatcccatt aagggagttg gtatacaaaa atgttaccct
     1261 gtttttatcc tgtgcccatg agcatactgt cattgatact ataattaaac tgccacatac
     1321 aattgttatc tgcttcgata tcatactcat agatatgcac tcctctataa aaaatagatt
     1381 tatcaacgat tgccattccg ggcttgcccg gaccaccttt gtcattctgg acctgttcca
     1441 gaatccagat atataacttt agatcccgac tcggcgttca ggatgactat cggttttacc
     1501 tcatcataac attgagcacc attgttatac tgaatcatga catgcatcca gtacaggttt
     1561 gctctggaat ttaaaaagca aactattgcg cttttatcct gtaggcatcc ctatcaaatt
     1621 gatcatatat ttttttattt ataaacagag caaccaattc tttgtcaagt ttattgttac
     1681 aggcttcttc ctcaattatt tttaacgcct gattaaaggt aattggtggt ttgtatggtc
     1741 tgtcggtagc aattaatgca tcaaagatat cagccagtgc cataatccgt gcctgcaatg
     1801 gtattgcatc gccttttaat ccataggggt accccgttcc atccagcttt tcatgatgct
     1861 ttatggctat ttcgggtatt tgactaaact cgggaggcca tggtatcgta cttacaaagt
     1921 tataggtgtg aataacatga ctttcaattt cgtgccgctc atcttcattt aagctgccac
     1981 gtttaatttt aagattcttt atatcatcat tagtaactat ctccattggn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttggatataa tttatgtgat tttaaaaaaa
     2161 tagcagtatc aatatatacc ttgccaaagt catgtaacag ggcagcatat tcaagttcct
     2221 ttaattgcgt ttcggtaaag gtaactgatg caaaagcctc atcgtgatct tcattgatgg
     2281 cgtttgcaat cttcaagcac atgtcagcaa ctctgaatga atggcctgat gtagcaacat
     2341 cacgtgattc aattgccgta accgatgcac gcacaaactc ttcaaactga ttctggatct
     2401 ggcgtatcat cctgttgttt tcaattgcaa tagcagcctg agaggcaacc gcttccatta
     2461 agctttcata ccgtttttgg aatggtacaa cctttgtttc aaaatcatcc ttgcttataa
     2521 gctttacaga atatgcttca ttccctttat aatcattgtt aaaatcttcc ttactattaa
     2581 ttaattgaat taccccaata gtctgattga tgtgattaac cattggcact acaagcattg
     2641 attttgaacg ataaccatga gctttatcaa atgatgaatt aaatgaaaca ggatcatcaa
     2701 ctcccaactg gtaaacatcc gggatgttca acacctgctg ggtaacagca acatatcctg
     2761 caatagagca tttatccata ggcatggaaa attcttcata tgggagttct tttgaaaatg
     2821 tatgtgaatg cttaaaacgc aataatttgt taccttcttt ttcttccaca atataaatac
     2881 ttccagcatc tgcaccagtt atggttttac ttaaatggag aataagacgt aacagcttgt
     2941 ccggattttt ttctatggaa agagccttgc caatattaat gagcgcttca aaatctcttt
     3001 cagtatcttc cagttcatgg agataatgtc gtgttaaact ctttatttca aaatgctgat
     3061 gagcgttata ttgtgaccgt tgtgcaatat ataaaaattc atccatggta atactatcgc
     3121 tataaaaagc cattaaacct tcaattgcat caaaacgttg atgtaaattg ccaacacata
     3181 catagttaat gtacacattt ttaccactta tatcaattgt gttcagaaca gttcctatag
     3241 attcataaca atctgaaggt ataatacata ctagagcata acaactattg acattggcaa
     3301 tgagcaaatg cagtatatca ggtaattcgt tataagggtg agaaacataa tggatggttg
     3361 catctatagt ttcaggagtt gagataactt tactgagtga agcatcgatg ataaaacaag
     3421 tatgtacagg taaggattcc aaaaataccc tcaatcatta ttaaattcca taaaacaaca
     3481 acaacaggat aagcaaattg tcaatgcaaa aattaaatgt caatttcttc agaaattaca
     3541 tgcaactctt ctttcttgta gtggtcagat ttttcatcag ttttttcctc aaactgtaaa
     3601 aatgtatctg cttcattcat tgtcgttgca gtaacatgca tctcttcaat tgtggaaact
     3661 ttttcattta ctgctggttc tgtaactatt tccatcccct gtcgtttctc aatttctttt
     3721 ttttcttcct cactctctat tattgccata tggtcacgtg atttaaaata ttttgctttt
     3781 gccgaaagtt gacttggctt ttgaacaatg atttcgccaa tgttaaactc taaattagaa
     3841 tcaccaagaa gtatattggt tgacttctcc atctttgaag aaatgtagcc taacatcctt
     3901 aaaagctcat caatgccaaa gttaagtttc accggttcat tgcttttaag cgaaacattt
     3961 ttttctataa gcttattctc cagcaatgcg taaattcgtg ttattggttt atcatacaat
     4021 tgcgattcta attgtatata ggtaaaccat attctgtgcg atagttcctt aaatatcaac
     4081 tgggttatgg cgggcatttg tttcataaca gctaccagcg attccttttt tattggtaac
     4141 agaatggttt ttccccagct tatggctgcc gcattgcgaa gtttgtccga aataagagcc
     4201 atttcaccaa atacatcacc cttcttcaaa accgacaaaa taatttcact ttcacttgaa
     4261 agttttataa tcttaacctt gccttctttg atgatataca tttcattacc gggttcatcc
     4321 tcgcaaaaga tcatctgctg atccataaat atcttatacg gaggttcatt aacaggaact
     4381 atcgaaccct ttacctttcc ttttatcttc tccattatgc ttttgacagc agtggcaaac
     4441 attccaccgg gatataattc tccataacgc ttgcagcagt acaatgcata cgcataactg
     4501 ttatttttat aaaaataatt gatgtgatta tacaatgcct cttcaggtgt ttccagttcc
     4561 acctctttat cctttaaggc aatcatcatc tcattatatg cgcgcagctc gtccgaaaag
     4621 tagtttaaca cttttatagc aatttgaggt gtctttgata aaagattaat aaattcttgc
     4681 ctggtcaatg atgtaataat gctatcagta agtgcaatag ccgattccat acggggttta
     4741 ttgcaaaggg atgcagtgac accaaaaaac tcgccacctt ttaatatgtt tttatatatc
     4801 ggtgcatttt tattataacg gcttaactgg acaacccctt tatctacaat gtaaacagta
     4861 tcagcgtctt catcaccttc gatataaata tatgctccct tcttaaaaag actctttttc
     4921 atatatcact acatccaata aacctgcatg gcatttttta atgtatcctg aacatctttg
     4981 ccagcaaaaa gtccagctaa tgtaagggca aatggtatag cacaagcagg gccctttgct
     5041 gttataatgg taccatctac tacaaccggt tcatttatta atgttgcacc aataagctct
     5101 ccttccagtc ccggaaaaca ggtagctttt ttaccatata aaatctgtgc cgaccccaac
     5161 acgataggag cagcacatat tgcagcaaca tagccgcctt ttgaaaaaat atgtttaata
     5221 aatttaataa catcatcatc tttttttaaa ttttcactcc ctggcattcc tccaggcaac
     5281 actatacaat tataatcgac aggattggct ttgtgcaata gaatatcagg ttttattgca
     5341 ataccgtgcg accccgttat cagtgaatcg gttaaccctg ctgaaacaac attaagattt
     5401 gctctccgca gtacatcgat tatggtaatg gcttcaatct cttcacatcc tggtgcaagt
     5461 ggcacaataa ttttcataat tttacctcct atataattat aacatggaat atctttttct
     5521 accttcgtca ttctgattag cacagtgctt tatgtcattc tgatggagca ttcgcaagca
     5581 tagaaactat tcttttataa aacgacgaga tttttcactc tgttaagaat gacatgacca
     5641 tacaacaaca cgattgttca gaataacttt ctacggctca gaatgacatt gctgtttagc
     5701 tgtaccagcc gtctgcttta ctgtagcagc aatgcatact atacctatag cataccatga
     5761 aagggcaata agtcaagtac atgtaactat cgctcataat tatttttgat gtaagcattg
     5821 aatgaggatt gaattagttt tatgtataat gtcttcgcaa taactttttt cttcctgagt
     5881 aaaatccgaa agaacataat ctgccacggg gatggcatcg gaaggccttc caacgccaat
     5941 acgtattcgg taaaaatcag gggtaccaac atgctgctgg attgaacgga ttccattatg
     6001 ccccttatga cccccaccaa actttaaagc aacattacca aacgtaagct ctatatcatc
     6061 atgcagaaca atcaaattat caggcaattc tttgtaatat gacagcacct tttgcacagc
     6121 aatgccactg ttattcatgt acgtttgcgg tatcatgaaa atacattcat taccgtcaat
     6181 tataccgctt ccggttagtg catcatgctc tttcttcctt actgttatac tatagagatg
     6241 cgctatagca acacccacaa gtataccgg
//
LOCUS       DAOOJH010000116         6262 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10232_length_6262_cov_8.727566, whole genome shotgun sequence.
ACCESSION   DAOOJH010000116 DAOOJH010000000
VERSION     DAOOJH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10232_length_6262_cov_8.727566"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..1081
                     /locus_tag="PLJ75_11135"
     CDS             368..1081
                     /locus_tag="PLJ75_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05807.1"
                     /translation="MEHIKKVRWLLIIMVIIGSIYLTYVYPDQVFRSNEFIQSDISPD
                     RLQFMPVTEDFRNYFVLQCINNTTSILIGDFTGTEKNISLAKDTNGDGKPDEVFEYFP
                     EIKKLTTPFKPTTSFFAGIEALKKDIILGDIYAVNYAYKMNSLPLLKAKLKSGRDVFK
                     FKHGYSVKIYDPDAPTTIMGEYFFGKKEGKYDLIFSTYYYKLYRTKISPPLSYSVYCK
                     DTTDKYIAEIVEELLKMVE"
     gene            complement(1096..1638)
                     /locus_tag="PLJ75_11140"
     CDS             complement(1096..1638)
                     /locus_tag="PLJ75_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05808.1"
                     /translation="MKKRKQYIINKKFQLKTTFSVIGIIFVIVAIIIAAIGVNAAYNN
                     KRLIHIIQIQDNIVEALIAYSQSPHDSEQKLAISNIANDHVKNIDTIKKIISLNNILL
                     LIIIGFVILQGVLLYFILIRKTHKIAGPLYVMSNYFNDIINGITPNPRSLRKQDELQD
                     FYALFVKMVDALHKREEGKK"
     gene            complement(1723..2331)
                     /locus_tag="PLJ75_11145"
     CDS             complement(1723..2331)
                     /locus_tag="PLJ75_11145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPD05809.1"
                     /translation="MINIVLGSSSPRRKSLLNNLFQDFTIIHPDIVETPHHGEAPLEF
                     VNRIASHKLMDIYHKVTYEHALIITCDTIVTIDNEILGKPYDEEHAYFMLLKLSGKTH
                     QVISSISMAYKNGHHNQKHNDYEITNVTFKLLSPADIKNYLSKINFLDKAGSYAAQEY
                     GEDIIQKIDGSLTNVIGFPLRCFFRMLIDMNILHNFLHYFKF"
     gene            complement(2334..4355)
                     /locus_tag="PLJ75_11150"
     CDS             complement(2334..4355)
                     /locus_tag="PLJ75_11150"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPD05810.1"
                     /translation="MKSRDIDTLIGAIQSNSINANIETIKRAYETANEAHKNQQRLSG
                     EPFIVHPMEVSIILANLKMDETTIIAALLHDTVEDTPIDINKVKELFGDEVALLVDGV
                     TKISSLKKHTKQKEQAETLRKMLLATIQDPRVIIIKLADKLHNMRTIMFQPENKQKYI
                     AQEVLDIYAPLAGRLGMSQIRSELEDLAFKVLYPDDYQAILEHIKARRTELESYIDTV
                     RNTLLQRLKELNIEAQVQGRIKHHYSIFKKLQQGRTFDDIFDLRAVRVITNEVKDCYG
                     ILGIVHTIWTPIPSRFKDYIAVPKSNMYQSLHTTVIGPDGHPLEIQIRTWDMHATAEM
                     GIAAHWMYKEGIKSFTPKYKSITILNNLHEWENEVRDTREFIKELKMDLYEDEIFVFT
                     PKGKIIKLAKGSTPVDFAYLIHTEIGNHCAGAKVNNQLVPLKTELKSGDIIEILTNKK
                     GHPSPSWLKFVKSSNARYKIRNWLRKNAEIEEEHLEKEGKQVSDKDKKEKIANVSIPE
                     KELVKIKSYNNKRKVGITINGTSNVMIKLSQCCQPIPGDNVIGFITRGRGITVHKRNC
                     PSLLRLQNESERFVDIKWEQSESTYPVKLAVEALDRPNLLKDVADEISLTKTNIIKAE
                     ASLVAKDQAMLKFVLEVKGINHLNDIIKRLKQIRNVTRVYKLNEKVVLK"
     assembly_gap    4362..4461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4612..5229
                     /locus_tag="PLJ75_11155"
     CDS             4612..5229
                     /locus_tag="PLJ75_11155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPD05811.1"
                     /translation="MIVLINETNPQKRFIKKAKEVLEEGGIVIFPTDTVYAYGCDIND
                     KRAIERLYQIKKIPRNKPLSFIFFDISEISKFVRNVSDSAFKIMKKAFPGPYTFIFQA
                     SKLVPKIAITNQKTIGVRIPDNPIALELVHALGHPIMTASVSTKEGNYVIDPVELDKE
                     YRNAVDMILSCGPKMSDPSTVVDFTTGQAQIVRKGKGQLFFIEEE"
     gene            5236..5559
                     /locus_tag="PLJ75_11160"
     CDS             5236..5559
                     /locus_tag="PLJ75_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05812.1"
                     /translation="MDAFISFLMKLLAIAIYIAMGALVAWIFYSLKRRDLFGGFIGGM
                     VIGVIGALIGGFILDKFLLDITIKILRFLVYDTGVNIIAAFIGGYVAVYIMNKLNHNK
                     ERKRY"
     gene            complement(5556..>6262)
                     /locus_tag="PLJ75_11165"
     CDS             complement(5556..>6262)
                     /locus_tag="PLJ75_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05813.1"
                     /translation="NNLKLQKDQLIEVIQLINDIGGTRVNDIHRLLPGINLIHGLIGE
                     HADTFKINYESLQEILHKGLLVKRINIRSLVPFPGTIAAQKQKKESKLLINRYRYYRQ
                     KIRHEIDHTMITKLYPAGTVIANMFVLSQVQGYSYAKEIRSYAITAKIPLELTLYSFL
                     DCIVVGHQERSLLCLPYPVNINTLPQKALATIPGIGKKTASAMIVQRPFNDFAQAMPY
                     LVSVPGKLIDAIKLCF"
BASE COUNT         1890 a         1020 c         1071 g         2181 t
ORIGIN      
        1 aatacttgca aagatgatat aaaaggttga acttcgcata cttatattcc tttttcatta
       61 gtatatcgtt attaatgttt taaaccaaat aattgttaca aaaatataca atgtattctt
      121 atgttacagg tgatcgttga ctataaaaaa agtatgtcta cttataaaca attttttatt
      181 aaattaaaaa aggtaatgga atatgtatgg caaacaattt tattttttaa aaattttaaa
      241 caataaaaat tatgaaactt aaaacattat tttgtataga aaaaattttt attattgact
      301 ttatttttta ttttgcatac aataaaatta tgatacacca tattcatagt taatcggggt
      361 tattataatg gaacatataa aaaaagttcg ctggttatta attataatgg taattattgg
      421 tagtatatac cttacgtatg tatatccaga tcaggttttt agaagcaatg agtttataca
      481 gagcgatatt tctcctgata gattacagtt tatgcctgtc acagaagatt ttaggaatta
      541 ttttgttctg cagtgtatca ataatactac ttcaatttta ataggagatt ttacaggcac
      601 tgaaaagaat attagcctgg caaaagatac caatggcgat ggaaagcccg atgaagtatt
      661 tgagtatttt cctgaaataa agaaattaac aactccgttt aagccaacaa cttcattttt
      721 tgcaggtatt gaagcattaa aaaaagatat aattctgggt gatatttatg cagtaaacta
      781 tgcctataaa atgaactcgc tgccgctttt aaaagctaaa ttgaaaagcg gaagggatgt
      841 gtttaagttt aagcatggat attcggtaaa aatttatgat ccggatgctc caacaaccat
      901 aatgggcgaa tatttctttg gtaaaaaaga agggaaatat gatctgattt ttagcacgta
      961 ttactataaa ctttaccgaa ctaaaatatc tccaccgctc agctattcag tttactgtaa
     1021 ggatacaacc gataaatata tcgctgaaat tgtagaggaa cttttaaaaa tggtagaata
     1081 atagtatata ccctgctatt tcttgccttc ttctcgcttg tgcaatgcat caaccatttt
     1141 tacaaacaga gcataaaagt cctgtaactc atcctgttta cgcagtgaac gcggattagg
     1201 ggtaatgccg ttaattatat cattaaaata atttgacata acatataatg gccctgctat
     1261 cttatgcgtt tttctgatta atataaagta taatagcacc ccttgcagta ttacaaaacc
     1321 tataataata agcaaaagga tattgttcag tgatataatc tttttaatgg tatcaatatt
     1381 cttaacatga tcattggcaa tatttgatat agccagtttt tgctcagaat catgcggcga
     1441 ttgagaataa gcaattaaag cctcaacaat attatcctga atctgaataa tgtgaattaa
     1501 tcgtttatta ttatatgctg catttactcc tattgcagca attatgatag ctacaattac
     1561 aaatataatg cctatgacag aaaatgttgt cttcaactga aattttttgt ttataatgta
     1621 ttgctttctc tttttcatat atataacctt ttatacaaat aaattttaaa cggctataat
     1681 agctatgaat aaataataaa ttaattttat tttatgtcaa tcttaaaatt tgaaataatg
     1741 taaaaagttg tgtaatatat tcatgtcaat tagcatcctg aaaaaacaac gcagtggaaa
     1801 tcctattaca tttgtcaatg aaccgtcaat tttctgaata atatcttctc catattcctg
     1861 ggctgcatag cttcctgctt tatccaaaaa atttattttt gatagatagt tttttatatc
     1921 agctggtgaa agcaatttga aagtcacatt ggtaatttca taatcattgt gtttttggtt
     1981 atgatgtcca tttttgtatg ccatgctaat agatgaaata acctgatgtg ttttgcctga
     2041 taattttaac agcatgaagt atgcatgttc ctcatcatat ggtttcccta aaatttcatt
     2101 gtcgatggtt acgatagtat cgcatgtaat aattaatgca tgttcgtatg ttactttatg
     2161 atatatgtcc attaatttat gagaagcaat gcggttaaca aattctaacg gtgcttcgcc
     2221 atggtgtggt gtttccacta tgtcaggatg gataatagta aaatcctgaa ataaattatt
     2281 tagtaaagat tttcttctag gggatgatga accaagtaca atgtttatca ctgttatttc
     2341 aatactactt tttcattaag tttatagacg cgggtcacat ttcttatctg ttttagcctc
     2401 ttgataatat cattcagatg gttaatgccc ttaacttcaa gtacaaattt tagcattgcc
     2461 tgatcttttg ccaccaatga ggcttcagct tttataatat ttgtttttgt cagtgatatt
     2521 tcatcagcaa catctttcag aagatttggt ctatccaatg cttccactgc cagttttacg
     2581 ggataagtgc tttccgattg ttcccatttt atatcaacaa aacgctcgct ttcattctgc
     2641 aatcgcagca gtgaagggca gttccttttg tgaacggtta ttcctctgcc acgggtgata
     2701 aatccaataa cattatcacc aggaattggc tgacaacatt gtgaaagttt tatcattaca
     2761 ttgcttgtac cattaatggt gattccaact tttcttttgt tgttgtaact tttaatttta
     2821 acaagttcct tttcagggat gctaacattg gcaatttttt cttttttatc tttatcactg
     2881 acttgcttac cttccttttc aaggtgctcc tcttctattt cagcattttt gcgcagccaa
     2941 tttcttattt tataccgtgc attggatgac ttaacaaatt taagccatga aggtgatggg
     3001 tgcccctttt tattggttaa tatttcaata atatcaccgc tttttaattc agtttttaaa
     3061 ggaactaatt gattattaac ctttgcacct gcacaatgat tcccgatttc tgtatgtatt
     3121 aaataggcaa aatctaccgg cgttgaacct tttgccaatt taataatttt accttttggg
     3181 gtaaatacaa aaatttcatc ttcatagagg tccatcttta attctttaat aaattcacgg
     3241 gtatcccgta cctcattttc ccattcgtga aggttgttaa ggatagttat acttttatat
     3301 tttggtgtaa atgatttaat cccttcttta tacatccaat gagctgcaat acccatctca
     3361 gccgtggcgt gcatatccca tgtgcgtatc tgtatctcca gcgggtggcc atcaggacca
     3421 atgaccgtag tatgtaacga ttggtacatg ttggatttag ggactgcaat ataatcttta
     3481 aatcgtgatg ggataggtgt ccatatagta tggacaatac ctaaaattcc atagcaatct
     3541 ttaacctcgt tagtaattac tctcactgct ctgaggtcaa atatatcgtc aaatgttcta
     3601 ccctgctgaa gttttttaaa aattgagtaa tggtgtttta tcctgccttg aacctgagct
     3661 tcaatgttaa gctcttttaa ccgttgtagc aaggtattgc gtacagtgtc aatatacgat
     3721 tccagctcag ttctgcgtgc tttgatgtgt tcaagtatgg cttgatagtc gtcaggatat
     3781 aatactttga atgctaaatc ttccaattct gaacgtattt gcgacatccc caatcgtccg
     3841 gctaatggag catagatatc aagtacttcc tgagctatat atttttgctt attctcgggc
     3901 tgaaacatta ttgtgcgcat attatgcagt ttatcagcaa gtttgataat aatgacgcgg
     3961 gggtcctgaa tagtagctaa aagcattttc cgtaatgttt ccgcttgttc tttttgcttg
     4021 gtgtgctttt ttaacgatga aatttttgtt acaccgtcta caagcagtgc aacctcatct
     4081 ccaaatagtt cttttacttt atttatatct ataggggtat cttcaacagt gtcatgcagc
     4141 aaagcagcaa tgatggttgt ctcatccatt tttaaattag ctaaaataat tgatacttcc
     4201 atgggatgaa caatgaaagg ctcgcctgaa agacgttgct gatttttatg agcttcatta
     4261 gctgtttcgt aagctctttt tatagtttca atatttgcat tgatgctatt tgactggata
     4321 gcacctatta atgtgtcaat atcgcgactt tttattttta annnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn ntaaaagaca ctcctattcg cacatttacc gggttgaagg
     4501 cttttgcagc aggcactcct gcaaaagcct tatatttttt agtagcatct actcttcaat
     4561 tgtctcaact aaaaaaagaa attatccgtt aattaatgta taataattat tatgatagtg
     4621 ttaatcaacg aaacaaatcc acaaaaacgc tttatcaaga aagctaaaga ggttcttgaa
     4681 gagggcggta ttgtcatctt cccaacagat accgtgtatg cgtatggatg tgatatcaat
     4741 gataagcgtg ccattgaacg tttatatcaa ataaaaaaaa ttccaagaaa taaacctttg
     4801 agtttcatat tttttgatat atccgagata tcaaagtttg tgcggaatgt atctgatagt
     4861 gcatttaaaa ttatgaaaaa agcatttcca ggtccctata cgtttatttt tcaagcatca
     4921 aaactggttc caaaaatagc tatcaccaat caaaaaacaa taggtgtcag gataccagat
     4981 aacccgatag ctctggaact tgtgcatgca ttaggccatc ctatcatgac agcaagtgtc
     5041 agcacaaaag agggaaatta tgttattgat ccagttgaat tggataaaga ataccggaat
     5101 gctgttgata tgatactgag ctgtggacca aaaatgagcg atccatcaac agttgttgac
     5161 tttacaaccg gtcaggcgca gattgtccgt aaagggaaag gacaactttt ttttattgag
     5221 gaggagtagt ttaccatgga tgcctttatc agttttctca tgaaattact tgcgatagct
     5281 atttatattg cgatgggtgc gctggtagca tggatatttt atagtctgaa gcggcgtgac
     5341 ctctttggcg gttttattgg cggtatggtc attggggtta ttggagcact tataggtgga
     5401 tttattctgg ataaattttt gcttgatatt accataaaaa tattgcgctt cctggtgtat
     5461 gatacaggtg ttaatattat tgcagcattt attggtggtt atgttgctgt ttatattatg
     5521 aataagctca atcataataa agaacggaaa cgctattaaa aacacagttt tattgcatca
     5581 attaatttcc ccggcacaga caccagatac ggcatagcct gagcaaaatc gttaaatggg
     5641 cgttgtacta tcattgctga tgcagtcttc ttccctattc cgggtattgt tgcaagagct
     5701 ttttggggaa gtgtgttgat attcacagga taaggcaggc ataataatga acgctcctga
     5761 tgaccaacca caatacaatc cagaaagcta tagagggtaa gctccagtgg aattttggca
     5821 gtgatggcgt agcttctaat ctcttttgca tacgaataac cctgcacctg tgaaagcaca
     5881 aacatatttg ctataaccgt gccagcaggg taaagctttg taatcatggt atggtcaatc
     5941 tcatgtcgta ttttttggcg ataataccgg taacggttta tcaatagttt gctttctttt
     6001 ttttgctttt gtgcggcgat agttcctgga aatggtacaa ggcttcgtat attaatacgt
     6061 ttaacaagta atcctttgtg cagtatttcc tgtaaggatt cataatttat cttaaatgta
     6121 tcagcatgct caccaataag tccatggatg agattgatgc cggggagaag cctgtgtata
     6181 tcattaacac gagtaccgcc aatatcgttg ataagctgta ttacctcaat caactgatct
     6241 ttctgcaatt ttagattatt ct
//
LOCUS       DAOOJH010000117         6261 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10235_length_6261_cov_7.861424, whole genome shotgun sequence.
ACCESSION   DAOOJH010000117 DAOOJH010000000
VERSION     DAOOJH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10235_length_6261_cov_7.861424"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2351
                     /locus_tag="PLJ75_11170"
     CDS             <1..2351
                     /locus_tag="PLJ75_11170"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HPD05814.1"
                     /translation="DIAQYYNYKDDSKNAKKTIQQAYEMLTAHRQYMTKETVRQYNEA
                     VQVIGGTVINEADYFHGKAGIAVVAMKLQGKDTKPVIAGLSEDIVAKNIISMGDAIVS
                     VNGKPVNAVDDIEHELNSYAYGADIVIQIEPVISHHVVIKNKSIGINNIDCSKQAAVA
                     TLTGHNDLVNSVAYSPDGRYIVSGSRDNTIKVWDARTYKEVETLRGHNRWVYSVAYSP
                     DGRYIVSGSEDATIKVWDARTYKEVATLRGHDGFVQSVVYSPDGRYIVSGSWDKTIKV
                     WDATTYKELATLIGHKDVVLSVVYSPDGKYIVSGSWDDSIKVWDAQTYTGVATLRGHN
                     GNVQSVAYSPDGRYIVSGSNDTTIKVWDARTYKEVATLTGHDNSVLSVAYSPDGKYIV
                     SGSWDDSIKVWDAQTYTGVATLSENYGHVYSVAYSPDGKYIVSGNLDTTVRIWDATIA
                     HTACNVANYKRLYHAVVQKGNVAEKEIYYKAKCYNSITIKNAHNDSVESVTYSPDGRY
                     IVSGSADRAIKVWDATTYEEVATLRGHDGFVQSVVYSPDGRYIVSGSQDKTIKVWDTR
                     TYKEVATLTGHTQLVLSVAYSPDGKYIVSGSLDHTIKVWDAKTYKEVATLSGHDGVVQ
                     SVVYSPDGRYIVSGSWDKTIKVWDAQTYTAVATLRGHNDNVNSVAYSPDGRYIVSGSR
                     DNTIKVWDARTYKEVATLTGHNDDVASVAYSPDGRYIVSGSSDTTIKVWDARTYKEVA
                     TLRGHDGFVQSVVYSPDGRYIVSGGFITIKVWDMQVILPKVF"
     gene            2515..2712
                     /locus_tag="PLJ75_11175"
     CDS             2515..2712
                     /locus_tag="PLJ75_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05815.1"
                     /translation="MAMPGLPEILIILMLLLILVWIVVVIKIIISSLSASRKVAWILC
                     SIVVLFIAIQFILFVMGILMR"
     gene            2833..3225
                     /locus_tag="PLJ75_11180"
     CDS             2833..3225
                     /locus_tag="PLJ75_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPD05816.1"
                     /translation="MKSIKLGKATSFGGFVASIIAIIGGIGWTLLVISLFSQRNEMEN
                     PVFYFFVLFGIIFIGVGIAIAVFSYKNLHSKNGYSLYDIVDSSEEKDAFHSSWDSTAA
                     KGTMNYCPHCGTSLRQDFIFCPGCGKKL"
     gene            3225..3695
                     /locus_tag="PLJ75_11185"
     CDS             3225..3695
                     /locus_tag="PLJ75_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05817.1"
                     /translation="MICTIVPVNLFATEYDVFCGSKHVAKFKKPALSLKSTCSITMND
                     KEYSIYREGVVSGDWILEEKHAGRVAVATKPSFLIDSFAIQYNNKAFQLKSKKLRLTN
                     QFIIYENDQQIGRIYNPSFFSRKLQCAIDGDSPLEFMIFAMYLASIIIDRRSSQ"
     gene            3884..4315
                     /locus_tag="PLJ75_11190"
     CDS             3884..4315
                     /locus_tag="PLJ75_11190"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPD05818.1"
                     /translation="MEIQNLTGLELMKSMIEGKIPPAPIAETIPMNGVDVAKGYIKFL
                     AKADKRHINPLGGVHGGFAATVLDSVTGCSVHTMLEAGAGYATIDLNIKMIKPVPLNR
                     ELIAEGKVIHLSHNIGISEGTLTDEDGKLYALATATCIIFK"
     gene            4378..4962
                     /locus_tag="PLJ75_11195"
     CDS             4378..4962
                     /locus_tag="PLJ75_11195"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPD05819.1"
                     /translation="MNANKTQQLLLKTGARLVHTKGFNNTGIQEILQCAGVPRGSFYF
                     YFKSKEEFGLALIEYYADMMCSMLRNYLTDASCSPIERIKLFFEGSARYYEQHKFEGG
                     CPVGNLSQEMSCLSEPMRVKLLSALRGMHSIIQQCIKEGQDAGQIDSSLDAGALAVFV
                     FNAWEGSLIDMKVSKSVYPLEICKTMVLRFIQSQ"
     gene            4981..6063
                     /locus_tag="PLJ75_11200"
     CDS             4981..6063
                     /locus_tag="PLJ75_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPD05820.1"
                     /translation="MEMYEQLRQVLDAHPATAPKTDTIIAILKMLFTPDEAAIAMNMS
                     FKVMDSEKIASLCSLPGEEAEKLLESMANKGIIYSKNKDGKKLYGLVPLIPGVFEFPF
                     MKSDGSERFQKLGRLWEQYHDEAFGVSFCGNPTPLMRVVPVEKSLSPQNRVHPYEEVR
                     NFIEQARYIAIADCACRTSVGKCDKPKDVCIIFDSAAQFLAERGFARQVTKAEAMDAL
                     DRSEKAGLVHTSNNSADRANLICNCCPCCCTVLRGRTQHNHPHAFEPSRFEAHVNDVN
                     CTGCGICVDERCPMKAITMRDGIAVVDTARCIGCGLCVTGCPETAIELRERASIPEIP
                     ATVVDMAMKVVTEKGRAEKFIEIMKR"
     gene            6077..>6261
                     /locus_tag="PLJ75_11205"
     CDS             6077..>6261
                     /locus_tag="PLJ75_11205"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670101.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HPD05821.1"
                     /translation="MTQKITVEKRGYILLIGLNRPEKRNAFDVDMYWDLAKAYGMLDA
                     DEDLRCGVLFAHGDHFTG"
BASE COUNT         1907 a         1020 c         1520 g         1814 t
ORIGIN      
        1 tagatatagc gcagtactat aactacaagg atgatagtaa aaatgcaaag aagactatac
       61 agcaggcgta tgagatgctg acagcccata gacagtatat gactaaagaa acggtacggc
      121 agtataatga ggcagtgcag gtgatagggg gtactgtcat caatgaggca gactattttc
      181 atggtaaggc tggcatagca gtggtggcaa tgaagctaca gggtaaggat acaaagccgg
      241 tcatagcagg tctatcagag gacatagtgg caaaaaatat cataagcatg ggggatgcga
      301 tagtatcggt taacggcaag cccgtaaatg cagtagatga tattgagcac gagcttaata
      361 gctatgcgta tggagctgat attgtcatac agattgagcc agtaataagt caccatgtgg
      421 ttattaagaa caagagcatt ggcataaaca acattgattg cagtaaacaa gcagcggtgg
      481 caacgctcac agggcataat gatttggtta attctgtagc gtatagcccc gatgggcggt
      541 atattgtatc aggaagtagg gataacacta tcaaggtatg ggatgccaga acctataaag
      601 aggtggaaac actacgaggg cataatcgtt gggtttattc tgtagcgtat agccccgatg
      661 ggcggtatat tgtatcagga agtgaagatg caactatcaa ggtatgggat gccagaacct
      721 ataaggaggt ggcaacacta cgagggcatg atggttttgt tcaatctgta gtgtatagcc
      781 ccgatgggcg gtatattgta tcagggagtt gggataaaac catcaaggta tgggatgcaa
      841 caacctataa ggagttggca acgctcatag ggcataaaga cgtggtttta tctgtagtgt
      901 atagccccga tggaaagtat attgtttcag gaagttggga tgacagtata aaggtatggg
      961 atgcacaaac ctatacaggg gtggcaacac tacgagggca taatggtaat gttcaatctg
     1021 tagcgtatag ccccgatggg cggtatattg tatcaggcag taatgataca actatcaagg
     1081 tatgggatgc tagaacctat aaggaggtgg caacgctcac aggacatgat aatagtgttt
     1141 tatctgtagc gtatagcccc gatggaaagt atattgtttc aggaagttgg gatgacagta
     1201 taaaggtatg ggatgcacaa acctatacag gggtggcaac gctctccgag aattatggtc
     1261 atgtttattc tgtagcatat agtcctgatg ggaaatatat tgtttcaggg aatttggata
     1321 caaccgtcag gatatgggat gcaacaatcg cacatacagc gtgtaatgtt gccaactata
     1381 aaagactata ccatgctgtt gttcaaaaag gcaatgttgc tgaaaaggaa atatattata
     1441 aggcaaaatg ttataattca ataactataa aaaatgcaca taatgatagt gttgaatctg
     1501 taacgtatag ccccgatggg cggtatattg tttcagggag tgctgataga gccatcaagg
     1561 tatgggatgc aacaacctat gaggaggtgg caacactacg agggcatgat ggttttgttc
     1621 aatctgtagt gtatagcccc gatgggcggt atattgtatc aggaagtcaa gataaaacca
     1681 ttaaggtatg ggataccaga acctataagg aggtggcaac gctcacaggg catactcaac
     1741 ttgttttatc tgtagcgtat agtcctgatg ggaagtatat tgtttcagga agtttggatc
     1801 acaccatcaa ggtatgggat gctaaaacct ataaggaggt ggcaacactc tcagggcatg
     1861 atggtgttgt tcaatctgta gtgtatagcc ccgatgggcg gtatattgta tcagggagtt
     1921 gggataaaac cattaaggta tgggatgcac aaacctatac agcggtggca acactacgag
     1981 ggcataatga taatgttaat tctgtagcgt atagtcctga tgggcggtat attgtttcag
     2041 gaagtaggga taacactata aaggtatggg atgccagaac ctataaggag gtggcaacgc
     2101 tcacagggca taatgatgat gttgcatctg tagcgtatag ccctgatggg cggtatattg
     2161 tttcaggaag tagtgataca accatcaagg tatgggatgc cagaacctat aaggaggtgg
     2221 caacactacg agggcatgat ggttttgttc aatctgtagt gtatagcccc gatgggcggt
     2281 atattgtttc aggaggtttt ataaccatca aggtatggga tatgcaggta attttgccaa
     2341 aagtttttta aataggtaat atttgttttg ggatttatta ccatatcggc agccatgcat
     2401 tgaagtatat atgtatggtg atagttatat taaatgtatg attaatgctt catgtttttt
     2461 atggttatat aatatcatgg atgtacacaa tatctctata agggggggta gtgtatggca
     2521 atgccaggtt tacctgaaat attaattatt ttaatgttac tgttgatact ggtatggata
     2581 gttgttgtga taaaaattat cattagttcc ttatccgcat caagaaaggt tgcatggata
     2641 ctgtgttcta ttgtagtatt atttattgct attcaattta tattgtttgt gatgggcatt
     2701 ctcatgagat aaacggtatt gtaatgattt cctcaagtta ataactgttg ttgcatcaat
     2761 atcataatca taatattttt tatatataat caggctgtat aaaaacaagc aattgagaac
     2821 aaggaggata ctatgaaaag tattaaactt gggaaggcaa catcatttgg aggatttgta
     2881 gcttctatta tagcaattat aggtggaatt ggatggacac tgctggttat atcattgttt
     2941 tcacagagaa atgagatgga aaatccagtg ttttattttt ttgttttgtt tggaattatt
     3001 tttattggtg ttggtattgc aatcgctgtt tttagttata aaaatctcca cagcaaaaat
     3061 ggatactcat tatatgatat tgttgacagc tccgaagaga aggatgcatt ccactcatca
     3121 tgggacagca cagccgctaa aggtactatg aactattgtc ctcactgcgg tacttcgctg
     3181 cggcaggatt ttatcttttg tcccggttgt ggaaaaaaat tataatgatc tgtacgatag
     3241 ttcctgttaa tctatttgca acagaatatg atgtcttctg cggcagcaag catgttgcaa
     3301 agtttaaaaa gccagccctt tctctaaaaa gtacatgcag tataacaatg aacgataaag
     3361 aatattctat ctatcgtgaa ggtgttgttt caggagattg gatactggag gaaaagcatg
     3421 ctggcagggt tgccgttgca acaaaaccca gctttttaat tgattccttt gcaatacagt
     3481 ataacaataa agcctttcaa ttaaaatcaa aaaagctacg tcttaccaat cagtttatta
     3541 tatatgaaaa cgatcagcag ataggaagaa tttataatcc atcatttttt tcgcgaaagc
     3601 ttcaatgcgc tattgatggt gatagtcccc ttgaattcat gatatttgct atgtatctgg
     3661 caagtatcat catagaccgc agaagttcac aataacaata gcaagctgta tatactataa
     3721 ccctgtgtat tgcagattga gaaatggtgt acaacaaaaa ataaaaaaat gtgttgacag
     3781 tagtagaccg gtctattact ataatataat gttcctgtgt ataaagcttt taagttttac
     3841 atggttatac cttacaattt gtttacgttt atggaaggga tatatggaaa ttcaaaattt
     3901 aaccggactt gaattgatga agagtatgat tgagggcaaa attccaccgg cacctattgc
     3961 tgaaactatc cctatgaatg gcgtagacgt tgcaaagggt tacataaaat ttttggcaaa
     4021 agctgataaa cggcatatca atcctctggg cggcgttcac ggtggatttg ctgcaacggt
     4081 gcttgactcg gttaccggct gttctgttca tacgatgctg gaggcgggtg ctggctatgc
     4141 aaccattgat cttaacatta aaatgataaa accggtgccg cttaacaggg aactcattgc
     4201 tgaaggtaag gtgattcatc tatcacataa cattggaatt tcagaaggca cccttacgga
     4261 tgaagatgga aagctgtatg ctcttgcaac ggcaacatgc atcatcttca agtaactatc
     4321 gaacatacat tatttgttgg cttttagtta acttgttacc atgatgattg aaatagtatg
     4381 aatgcaaaca aaacacaaca gttgctgctt aaaacaggtg ctcgtcttgt acacacaaag
     4441 ggtttcaata ataccggcat tcaggaaatt ttacagtgtg ctggtgttcc cagaggctct
     4501 ttttattttt atttcaaaag taaggaagaa tttgggttgg cgcttattga gtattatgca
     4561 gatatgatgt gcagcatgtt gcgtaactac ttaaccgatg caagctgtag tcctatagaa
     4621 aggattaagc ttttttttga agggagtgcc cgttactatg agcaacataa gtttgaaggt
     4681 ggttgtcctg ttggtaacct ttcgcaggag atgagctgcc ttagcgagcc aatgcgtgta
     4741 aagttacttt ctgcattgcg aggtatgcac agtattatac agcagtgtat caaagaagga
     4801 caggacgctg gtcagattga tagcagtctt gatgcgggag cactggcagt ttttgttttt
     4861 aacgcatggg agggctcgct tattgatatg aaggtttcaa aaagtgttta tccacttgag
     4921 atatgcaaaa ccatggtgtt gcgttttata caatcacaat aatacaactg gaggctttca
     4981 atggaaatgt atgaacaact tcgacaggta cttgatgctc atcctgccac tgcaccaaag
     5041 acagatacca tcatcgcaat actcaaaatg ctttttactc ccgatgaagc tgcaattgca
     5101 atgaatatga gctttaaagt catggatagc gaaaagattg catcgctgtg ttcattgccc
     5161 ggagaggaag ctgaaaagct tcttgaatca atggctaaca agggtatcat ctattcaaaa
     5221 aacaaggatg ggaagaaact gtatggattg gtaccgctta tccccggtgt ttttgaattc
     5281 ccatttatga aatctgacgg ttcggaaagg tttcaaaagc ttggcaggct atgggaacag
     5341 tatcatgatg aagcctttgg tgtttcgttt tgtggcaatc caactcctct catgagggtt
     5401 gtgcctgttg aaaaatcatt aagtccccaa aaccgggtac atccttacga ggaggtaaga
     5461 aattttattg aacaggcgcg ctacattgcc attgcagact gtgcatgcag aaccagcgtg
     5521 ggtaaatgtg ataagccgaa ggatgtgtgt attatttttg atagcgccgc ccagttttta
     5581 gccgaaaggg gatttgctcg tcaggtaaca aaagctgaag caatggatgc tctggatagg
     5641 tctgaaaaag caggattggt ccataccagc aacaatagtg ctgaccgtgc caaccttatt
     5701 tgcaactgtt gtccgtgctg ctgtacggtt ttacgtgggc ggacacagca taatcatccc
     5761 catgcgtttg agccaagccg atttgaagcg catgtcaatg acgtcaattg caccggctgc
     5821 ggtatatgtg ttgacgagcg ttgccctatg aaagctatta ccatgcgtga tggtatagcc
     5881 gtagttgata ctgccaggtg cattggatgc ggattgtgtg ttaccggatg tcccgaaaca
     5941 gcaattgaac ttcgtgaacg tgcatctatc cctgaaatac cggctaccgt tgttgatatg
     6001 gcaatgaagg ttgttacgga aaaaggaaga gcagaaaaat ttattgaaat tatgaaacga
     6061 taacaaggag gcgttaatga cgcaaaagat tacagttgaa aagagaggat atattttact
     6121 gattgggctc aacaggccag aaaaacgcaa cgcttttgat gttgatatgt actgggattt
     6181 agcaaaagcg tatggcatgt tagatgctga tgaagacttg cgctgtggag tactttttgc
     6241 tcacggtgat cattttacgg g
//
LOCUS       DAOOJH010000118         6223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10313_length_6223_cov_9.485409, whole genome shotgun sequence.
ACCESSION   DAOOJH010000118 DAOOJH010000000
VERSION     DAOOJH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10313_length_6223_cov_9.485409"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..703
                     /locus_tag="PLJ75_11210"
     CDS             296..703
                     /locus_tag="PLJ75_11210"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HPD05822.1"
                     /translation="MISFDLQCANGHLFEGYFKDYDAFDKQLNAGLVECPLCNSREIK
                     RLFTGCSIQTKNSFSDKKQPSFFEMIRMVESYIKQHFENVGNEFPEIARAIYYGIEEE
                     RNIYGTASREDMEELLDEGIPILPVPKIDDVEQ"
     gene            complement(711..1193)
                     /locus_tag="PLJ75_11215"
     CDS             complement(711..1193)
                     /locus_tag="PLJ75_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05823.1"
                     /translation="MMDAVIDFFAKILSYCVQFINRVADLLLRLYEYCIGIFRELEIF
                     EKCIIIVSIVSLVIPVLPIARFYIFESWYYVNNPLAIYFIGIIIIMVVSALWQKFWIL
                     LARLLIILYYCAWIIYLPIGNCITKARPYELAYGYYCNIVVSVVYIVLCVLSLINSRR
                     "
     gene            1567..1639
                     /locus_tag="PLJ75_11220"
     tRNA            1567..1639
                     /locus_tag="PLJ75_11220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1600..1602,aa:Ala,seq:cgc)
     gene            complement(1753..3990)
                     /locus_tag="PLJ75_11225"
     CDS             complement(1753..3990)
                     /locus_tag="PLJ75_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPD05824.1"
                     /translation="MKRKTLTMSICLIVIVIVLCSGIYRFFFSQSHRDTITAPISDPV
                     CIYRLDKEYPFIQAKTRDDAFFGLGFAHARDRLRQIEFLRIMAHGTSVHNGISHYEAI
                     DKLVLAVGLHKKAATITRELSPTYKNYLQRYVDGINTYKEKWAVGNASQPWEVADVVR
                     ISLFLEFANAFLTNTELFFPVHDGDYPLMSREIHNKKIARSYSPDDDTIIRQIKKLRN
                     ALASLFGLYGSYDRGFAIALPARVMDTDNATLCLSYEHSISIYPLIYPVIIQIEKNTI
                     QGYTFAGLPYIIAGETDAVRFASFSVMADTQIFCKHPTRTNNTIVYYQSATGYKEFLK
                     EQSLNYVSRFTDTGAVISDAFEESVKDTVIALAYISPEKGYFNAMLDAPFATSCNDYI
                     NTVKDYKGQPRIFVVQDGVTSFQIIAGNTITDNDSPVLATPQKALIAKTVANLFDVQQ
                     MDMLVAGSEMIENPSFAVQKMMAFNNLMRLKTIQDAMSQVAIYRNNDVASLLTDIKYP
                     AAQQYLSIMNNLLGKMPITSAKLANIYFQEWNFTMDKDYVAPTLYNAIITSLVKETFT
                     DELPYTVGDMLQYYMIFEPEFLRIFQEDKSPYFDDTATVGKLESRDLIFDRAFLHSLN
                     YFNYTIGPYIDTWRWGKVNRGIVDDKTQGSNVVNRVKKKKTISLSGFDNTVFRNLIKR
                     QDYSIDSITVCASYMDENTHRMTALISPVMDVMPSYYTDINANTFFTINVNNKHDLFT
                     INPVK"
     gene            complement(4007..5239)
                     /locus_tag="PLJ75_11230"
     CDS             complement(4007..5239)
                     /locus_tag="PLJ75_11230"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HPD05825.1"
                     /translation="MESYIQRLFAERIGGKNFGKEDKIYKFEKIKMARREALKNNPGR
                     ELIDLGVGEPDEKAFDIVIETLCKEAYKPENRGYADNGIPEFKYAVSNYLKQVFDVDG
                     IDPETEVNHAIGSKSALAMLPSAFINPGDVCLMTVPGYPVFGTHAQWYGGEVYALPLL
                     KQNNFLPDIYAIPDNIVKRARVIVINYPNNPTGAVATEDFYKEVIEFALKNSIVVVQD
                     AAYAALTYDGKPLSFLHIPGAKEVGVEIHSLSKAFNMTGWRMAFVAGNSLVVKAFATV
                     KDNYDSGQFKAIQKASITALEHPELTEKIKAKYKRRLVSLARTLQRLGFDAHMPGGTF
                     YLYVEVPGRTKNGIVFDNAEAFSQYLIKECLISSVPWDDAGHFVRFSATFEAKDEKDE
                     QRILQEVENRLGALAFEF"
     gene            complement(5413..5868)
                     /locus_tag="PLJ75_11235"
     CDS             complement(5413..5868)
                     /locus_tag="PLJ75_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05826.1"
                     /translation="MDSKKCMLLFVCFIIGCTSMKIWVPSYTFDEVIAQKNAIDAAQN
                     PAEGYMLLKELEKKRVIIKKAVVKEVVPSTNIDYNFCVVATVDHPSGSIECHIHTRNW
                     RNEEDIDSIAKLKPGDTIHVVGVFKRFLKLLGQQYVVEIVEANISKVKQ"
     gene            complement(5980..>6223)
                     /locus_tag="PLJ75_11240"
     CDS             complement(5980..>6223)
                     /locus_tag="PLJ75_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD05827.1"
                     /translation="GWYLLLENIVKPIVFGEKLKFHPLILFFLLIGSLQTFGLPGIII
                     GPILLTLFFSFWEMYKILDSYTTEQDKKIKKPHKNS"
BASE COUNT         1947 a         1271 c         1043 g         1962 t
ORIGIN      
        1 ttatttttct attgcaagca tttttttaaa gatttgttca gaggtctcaa ttataaaata
       61 atattacgta ttcctcattt ttactttttg atttttttgc catggtttag agttatggcg
      121 atacaaatga tgtactttgc tactatttta tataactatc tttcttgaca ttatgcaact
      181 gcccaatcaa cttttgaata attgcctcta tcacaattat atattttttt tgaaatgtaa
      241 ttgaaaaact attacaattg ttgccagata gttattatta atgaagtgag ttgatatgat
      301 aagctttgat ttacaatgtg ccaatggcca cctatttgaa ggatatttta aagattatga
      361 tgcgtttgac aaacaattga atgctggtct tgtggaatgt cctttatgca attcgcgtga
      421 aattaaaaga cttttcaccg gatgttccat acaaacaaaa aattcatttt cagataaaaa
      481 acagccatct ttttttgaga tgatacggat ggtcgaatca tacataaaac aacattttga
      541 aaatgttggt aatgagttcc ccgagattgc ccgtgccata tactatggca ttgaagagga
      601 acgaaacatt tatggaactg catcccgtga agatatggaa gagctccttg acgaaggaat
      661 accaatcctg cctgtgccaa aaattgacga tgtagaacaa tgaagggttg ctatcgcctg
      721 ctattaataa gcgatagcac acataaaact atatagacaa ctgaaacaac aatattacag
      781 taatatccat atgccagctc atatggtcgc gcttttgtga tacaattgcc aatagggaga
      841 tagattatcc atgcacagta atacagtatt atgagtagtc gtgccagtaa aatccagaat
      901 ttttgccata gagcagaaac aaccatgata atgataatcc cgataaagta tatggcaagc
      961 ggattattga cataatacca gctttcaaag atataaaaac gcgctatagg caaaacgggg
     1021 ataaccagag aaacaattga tactataata atacattttt caaaaatttc aagctcgcgg
     1081 aagataccaa tgcaatattc atacaatcgc agaagaagat cagcaacccg attaataaac
     1141 tgaacacaat atgaaagtat ttttgcaaaa aaatctatta ccgcatccat catatttctc
     1201 ctcttgcaat tctggtagtt gtactaccaa atgttagtat ccataaaaaa atacaatgat
     1261 atttcattat ctaccggatg gcctttcatt ttttaaaagt tctgcaaaca attcatcaat
     1321 tcataacgat actatccgca acaatgaaac ggatttatac gaaatatttc cattggaaac
     1381 gcatagttat tataagcata ctttcataaa aattgctcta tgataaggtt atgaaaatat
     1441 tttttgtctt caccaggagc tgtggcacca tgatgaaatc cttttatacc catacctgcc
     1501 actaaaaaat ctattgacga aaaacatgat ttacccttcc atgattaata tactatatat
     1561 tcttttgggg ctgtagctca gctgggagag cgctagaatc gcactctaga ggccgagggt
     1621 tcgactccct tcagctccac aggcatagta taaatatttt ttgtcctttc attgcgcttc
     1681 atgcatgcac agctcaatac atactcaaac aacatctcat actgcaaccg tgttgtgtgc
     1741 ctctaccaaa atttatttaa cagggttaat ggtaaaaaga tcgtgcttat tattgacatt
     1801 gatggtaaaa aaagtattgg cattaatatc ggtataatag gatggcatca catccataac
     1861 gggactaatc aatgctgtca tccggtgtgt gttttcatcc atatacgatg cacatacagt
     1921 aatgctatcg atactataat cctgcctttt gatcaaattc ctgaaaacag tattatcaaa
     1981 gccgcttaat gatatggtct tctttttctt cactctgttc actacatttg aaccctgcgt
     2041 tttatcatca actatccccc tattaacttt accccatcgc catgtatcaa tatatggtcc
     2101 aatagtatag ttaaaataat ttaaagaatg caaaaacgcc ctgtcaaaga taaggtcacg
     2161 tgattccagt tttcccacag tagcagtatc atcaaaatag ggtgatttat cctcctgaaa
     2221 aatccttaaa aattctggtt caaagatcat ataatactgc aacatatcac ctacagtata
     2281 cgggagttca tcggtaaatg tttctttaac aagcgatgtt atgatggcat tatacaatgt
     2341 aggcgctaca tagtctttgt ccatggtaaa attccactcc tgaaaataaa tgtttgccaa
     2401 tttagccgat gtaatgggca tttttcccag aagattgttc attatagaca ggtattgctg
     2461 tgcagcagga tatttaatat cagtaagcaa tgatgcaaca tcattattcc ggtagatagc
     2521 aacctgtgac atggcatcct gtatagtttt taatcgcatc agattattaa atgccatcat
     2581 tttctggaca gcaaatgatg gattctctat catttcactg cctgctacaa gcatatccat
     2641 ctgttgaaca tcaaaaaggt tggctaccgt ttttgcaatc agcgcttttt gtggtgttgc
     2701 aagcacaggg ctatcattat ctgttatggt attgcctgcg attatctgaa aagaggtcac
     2761 cccatcctga actacaaaaa ttcttggttg acccttatag tcttttacag tattgatata
     2821 atcattgcag gacgtcgcaa atggtgcatc aagcattgca ttaaaatatc ccttctctgg
     2881 acttatatag gcaagtgcaa taacggtatc ttttactgat tcttcaaatg catccgatat
     2941 aaccgcgcca gtatcagtaa atcgtgacac atagtttaat gattgttctt tcaaaaactc
     3001 tttatagcct gttgcacttt gataataaac aatggtattg tttgttcgcg tggggtgctt
     3061 gcaaaaaatc tgtgtatcag ccatcacaga aaatgatgca aagcgcacag catccgtttc
     3121 ccctgcaata atatatggca atcctgcaaa ggtataaccc tgaattgtat ttttttcaat
     3181 ttgtataata accggataga ttaaggggta tatagaaatg ctgtgttcat agctcaaaca
     3241 tagtgtagca ttatcagtat ccatgacacg cgctggtaat gcaatagcaa atcccctgtc
     3301 atacgaacca tacagcccaa aaagtgaggc aagtgcattt cgtagttttt ttatctgtct
     3361 gattattgta tcatcatcag gggaataact gcgagcaatt tttttattgt gaatttcacg
     3421 actcatcaat ggatagtcac catcatgaac ggggaaaaac agctcggtat ttgttaaaaa
     3481 tgcattggca aattcaagga acagggatat gcgcacaaca tccgcaacct cccatggttg
     3541 tgatgcattc ccaactgccc atttttcttt ataggtgttg atgccatcga cataccgttg
     3601 taaataattt ttataggtgg gcgatagttc cctggtaatt gttgcagctt ttttatgcaa
     3661 ccccaccgcc agaacaagct tatcgattgc ctcataatga gaaataccgt tatggacaga
     3721 tgtgccatgt gccataatcc gtaaaaactc aatctgcctt aatctatcac gtgcatgagc
     3781 aaagcccaat ccaaagaagg catcatcgcg agtttttgct tgaataaacg gatattcttt
     3841 atctaaacga tagatgcata caggatcgct tattggtgct gtaatggtat ccctgtgcga
     3901 ttgtgagaaa aaaaatctat atatgccaga acagagcact ataacaataa ctatcaaaca
     3961 gatgctcatt gttaatgttt tccttttcat ggttaacaat ccatgcttaa aattcaaatg
     4021 caagtgctcc taatctgttt tctacttcct gtaaaatcct ttgctcatcc ttctcatcct
     4081 ttgcctcaaa tgttgccgaa aaccgcacaa aatggccggc atcatcccat ggcactgacg
     4141 atatcaggca ctctttaatc aaatattgtg aaaaagcttc agcattgtca aaaacaattc
     4201 cattttttgt tcgtccggga acctcaacat aaagataaaa ggtgccgccc ggcatgtgcg
     4261 cgtcaaaccc taatcgctgt aatgttctgg caagcgatac taatcggcgt ttatattttg
     4321 ctttaatttt ttcagtaagt tcaggatgtt ccagcgctgt tatagatgcc ttttgaattg
     4381 ccttaaattg acccgaatca taattatctt tgacagtagc aaatgctttt accacaagac
     4441 tgttgccagc aacaaatgcc atacgccatc ccgtcatatt aaatgctttt gaaagcgaat
     4501 gtatttcaac tcccacctct tttgcaccgg gaatgtgtaa aaatgacagc ggtttgccat
     4561 cataggttaa cgctgcatag gcagcatcct gcactaccac aatactattt tttaatgcaa
     4621 attctataac ctctttataa aaatcttctg ttgccacagc accggttgga ttgttaggat
     4681 aattaataac aatgactctg gctctcttta caatattgtc aggtatagca tagatatccg
     4741 gtaaaaaatt attctgcttg agcaatggca gagcatacac ctcgccgccg taccactgtg
     4801 catgtgttcc aaaaacagga tagccgggta ccgtcataag acatacatca ccaggattaa
     4861 taaaagcaga gggcaacatg gcaagtgctg attttgaacc aatagcatga ttgacctcgg
     4921 tttccgggtc aatgccatca acatcaaaaa cctgctttaa atagttacta acagcatatt
     4981 taaattcagg tatgccgtta tcagcatagc ctctgttttc aggcttatag gcttctttac
     5041 ataacgtttc tataactata tcaaatgcct tctcatcagg ctcgccaaca cctaaatcaa
     5101 tcagttccct gccaggatta tttttcagtg cctcgcgcct ggccattttg atcttttcaa
     5161 atttataaat tttgtcttct ttcccaaaat tttttccacc tatccgttcg gcaaataacc
     5221 gctgaatgta cgactccata acgatccatc ctcctgaaaa gtaaaatcat taacctcacc
     5281 ccggcctctc tccttcgcaa ggcgagggga gcattgtccc ccatccttat ataaggaaag
     5341 gcatgaaggt tgaggtttgc aatatgttca tcccatttga ccgcatagca atgcgcttct
     5401 atatacccat catcattgtt ttacctttga aatgttagcc tctactatct ctacaacgta
     5461 ttgctgtcct aacaatttta aaaatcgttt aaaaacaccc actacatgga tagtatcacc
     5521 tggttttaac tttgcaatac tgtcgatatc ttcttcattt cgccaatttc tggtatggat
     5581 atgacactca atgcttcctg acgggtgatc caccgtggca acaacacaaa aattataatc
     5641 tatgtttgtt gaaggtacaa cctcttttac caccgctttt tttataatga cacgcttctt
     5701 ttcaagctcc ttcaaaagca tatagccttc tgccgggttc tgggcggcat caattgcatt
     5761 tttttgtgca atcacttcat caaacgtata ggacggcacc catattttca tagatgtaca
     5821 gcctataata aaacaaacaa atagcagcat gcatttttta ctatccatta tcattacctc
     5881 gggaattgtt gtagcaacag gttgtcaagg atagcaccac tactattttt ttgtcaaata
     5941 tctttataca aatacaataa attatgagta actatcgccc taactatttt tatgtggttt
     6001 ttttattttt ttatcctgct ctgtggtgta actatccagt attttataca tctcccagaa
     6061 cgagaagaaa agggtaagaa gtatgggtcc aatgataatg ccgggtaaac caaacgtttg
     6121 taaactgcct atcaataaaa agaataaaat taagggatga aactttaact tttcaccaaa
     6181 aacaataggc tttacaatat tctcaagcag caaataccag cct
//
LOCUS       DAOOJH010000119         6109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10532_length_6109_cov_8.868352, whole genome shotgun sequence.
ACCESSION   DAOOJH010000119 DAOOJH010000000
VERSION     DAOOJH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_6109_cov_8.868352"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLJ75_11245"
     CDS             <1..772
                     /locus_tag="PLJ75_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPD05828.1"
                     /translation="GIKNDNLEMDIDIKTRDYGMSFLLSYAKLDADIICVLHPLSYLI
                     KKSNFNLLKNFTKNYKLVNGVIIDSGTFKETSKSISFPIIIALYKKEKTGMDYNYVQN
                     YKFKTIDNKNFSLNDFDFISNYINKYPLKNIKPNENDILFWTMRDINALKRNRTFIHE
                     FGYNAIIVDKNKLDYYIYVDVFKQFSHLIPYYFGNLDVFINNEMFNKYKDYFIYECIM
                     RNNFLKKYVQYDDNISLEKAKEIIINYFKFLTGEHYVD"
     gene            1097..2182
                     /locus_tag="PLJ75_11250"
     CDS             1097..2182
                     /locus_tag="PLJ75_11250"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05829.1"
                     /translation="MERLSEEIKVYKDITPENYVFSLTNDDARMKIQEIAQYLYRIKV
                     LFAHDSVSESQAYRILARLVDEQIEITGKGKGVKVKVSNELRSSCIQSPYDPDATYRE
                     KGKQASQGYSVSIVETCDPDNKLQVITHVITEKNNVDDSTILEDNFEAIVGEDTEELI
                     ADGAYANKSVQDRLSESKKTLITTAIRGRKPDSNKVRSTDFEIENNRIIRCPYGEIPI
                     HQEYNNGVIIAHYAHESCSSCPLKCMIRKNKRKPHVLVITQERLQMDRQREKYNDSEY
                     LKKCALRPAIEGTMFQFKLHLRNGKSRYRGKSKVKCSSILRTIAINFKRVYAYRLKEA
                     LSYFILRNSMIIKSFLARKNILCCFST"
     gene            2304..3335
                     /locus_tag="PLJ75_11255"
     CDS             2304..3335
                     /locus_tag="PLJ75_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05830.1"
                     /translation="MEKYTMFSNTDFHFGHTSINEDIAYFYTKMNDAIINLVRSLPVS
                     VQTSAMIFLMHNAGIQPGQNLDFFSNYYSPIWSIIPHIMIRHTCTRIKQEFMYLAATA
                     HAMVMLLHSLDNHINDGQVVPDHLTLLIRSQGWKLFWDTISTFCSLIHTDKEIVNSCL
                     DRYYACITTDKSIDNLEEYLRIFRDQMATALIVPLLTVQFVQDMSLDEDIRALLECFG
                     LAWRIYDDIQDMKDDLNNGRNSAVTLSLPREVRCRWNEFLKIGTIIPEGENISERKND
                     SINILEEMIQTSGVIPILTKRIIKELKAAAKHAEDIGLFGLSDEIVKLRIHFVQGMKC
                     DTGEDNVSD"
     gene            3322..4365
                     /locus_tag="PLJ75_11260"
     CDS             3322..4365
                     /locus_tag="PLJ75_11260"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD05831.1"
                     /translation="MSVIDSIPFEILEAVKKENLSIEVTTRCTSNCTYCFVLSDCIEY
                     HDLDYETVFSIIMEGYEIGYRHLHITGGEPLLWGYLFDILNDAISIGYEAIFLNTNGF
                     LLDTKILQQLKSIKGLSLSISLLGFEAIHNAFRGNNSFIKTTQAIEMALYNNIPVYLF
                     CVTGKSLLPHVPHFIQWAYQKFPGIKEITLIQLIRVSEDAGNVCNELLSPDDFLFLVR
                     MISLLKIYGFPVSLLENPLAAVVAQLFQLPWFPNPLHLIRPGKLTIKADKSITIAHSS
                     SQSVGIYSRGELHRILASEVYLRGVKEDHNTCSSCNHIAACRKAGMQRPSEFFRDMNE
                     NIPYCKRVLDSIC"
     gene            complement(4462..5061)
                     /locus_tag="PLJ75_11265"
     CDS             complement(4462..5061)
                     /locus_tag="PLJ75_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD05832.1"
                     /translation="MARKLRVQLPGLTYHITSRCIEWKNYLQEDYFKACFVQVLNKAK
                     AKYHFKLIAFCIMDNHIHLVIHTLDDGAPISRIVQYIKARFAELYNKITGRTGPFWNE
                     RYKDSIVQFAKDGFHYLLWLLWYLAYNPIRRRLCSDPTKYHYSSIQAYLTEEANIGVP
                     IDHHDYFVKLGETFAKRVARFMQYEEYYRKKYSMILEWV"
     gene            5462..5623
                     /locus_tag="PLJ75_11270"
     CDS             5462..5623
                     /locus_tag="PLJ75_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05833.1"
                     /translation="MGKIGGQSIVKVIVQKRKKGSEHKGPGNLLLMAYMADSFAGFQV
                     KDLIGEVIE"
     gene            5782..>6109
                     /locus_tag="PLJ75_11275"
     CDS             5782..>6109
                     /locus_tag="PLJ75_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HPD05834.1"
                     /translation="MKACVTGATGFIGGNLVKALLAKKHSVKALILPNDPKIPELKKN
                     KVEVVQGDIRDLESVKKAVKGCDVIFNCAAVVTDWAPQRLFEEVTVGGTKNVCEASVW
                     AKVKRLV"
BASE COUNT         2128 a          879 c         1216 g         1886 t
ORIGIN      
        1 aggtataaaa aatgataatt tagaaatgga tattgatata aaaacaaggg attatggaat
       61 gtcttttttg ttatcatatg caaaattaga tgcagatata atttgtgttt tacacccttt
      121 gtcttatttg attaaaaaat ccaattttaa tttattaaaa aattttacaa aaaattataa
      181 actagttaat ggagttatta tagatagtgg aacatttaaa gaaacttcaa aaagtatttc
      241 tttcccaata ataatagctt tatataaaaa agaaaagact ggaatggatt ataattatgt
      301 tcaaaattat aaattcaaaa caattgataa taaaaacttt tccttgaatg attttgattt
      361 tatttccaat tatattaata aatatccatt aaaaaacatt aaaccaaatg aaaatgatat
      421 attgttttgg acaatgagag atataaatgc tttaaaaaga aatagaactt ttatccatga
      481 atttgggtat aatgctatta ttgtagataa aaataaatta gattattata tttatgtaga
      541 tgtatttaaa caatttagtc atttaattcc atattatttt gggaacttag atgtatttat
      601 aaataatgaa atgtttaata aatataaaga ttatttcatt tatgaatgta taatgagaaa
      661 taatttttta aaaaaatatg ttcaatatga tgataatatt tctttagaaa aggcaaagga
      721 aattataata aattatttta aatttttaac tggagaacac tatgtagatt aaaaaaatta
      781 attaggcagg tttcattttg atcatttgtt tatactggcg atggggttaa acaggattgg
      841 tgagaggaca atatcagaac gagcatttta ttatatgcgc cgacgtgttg ttgattatga
      901 ggaaaagaca gggataaatc tatttgagaa agtatttgta catttgaggg atgattatat
      961 aaagaaattt gggattagtc agaaagtcaa gaggattgat tcaacgttaa ttggttcaaa
     1021 tattcgcaga ctaaaccggc tcaaactgtt tttagaggtg ttgaattatt ttttgaagca
     1081 tagtgatgag agtattttgg agaggttatc ggaagagata aaggtatata aggatataac
     1141 acctgagaat tacgtatttt ctctgacgaa tgacgatgca aggatgaaaa ttcaggagat
     1201 tgcacagtat ctttaccgta tcaaggtgct atttgcccat gattctgtga gtgaaagcca
     1261 ggcatatcga atacttgcac ggctggttga tgagcagata gagataacag ggaaagggaa
     1321 aggggtaaag gtaaaagttt caaacgaact aaggagcagt tgcatacaat caccgtatga
     1381 tcctgatgcg acatatcggg aaaaagggaa gcaggcaagt caggggtatt cggtttcaat
     1441 agtagagaca tgcgatccag acaacaaact ccaggtgatt acgcatgtga taacagaaaa
     1501 aaacaatgtt gatgacagta caatacttga agacaatttt gaagcaatag tgggggagga
     1561 cacggaagaa cttattgccg atggagcata tgcaaacaaa agcgtacagg acaggctatc
     1621 ggaatcaaag aaaactctta ttacaacagc aatacgggga agaaagccag acagcaacaa
     1681 agtacgaagt actgactttg agattgaaaa caatcgcata atacgctgtc cctatgggga
     1741 aattccgata catcaggaat ataataatgg tgttatcata gcacactatg ctcatgagag
     1801 ttgttcctcc tgtccactga aatgtatgat acggaaaaac aaacgtaagc cgcatgtgct
     1861 tgtgattaca caagagagat tacagatgga taggcagcga gagaaataca atgatagtga
     1921 gtatttgaaa aaatgcgcac ttcgaccggc aattgaaggt acaatgtttc agttcaagct
     1981 gcatttgaga aatgggaaaa gtcggtatag agggaaaagt aaggtaaaat gcagttcgat
     2041 actgagaaca attgcaataa atttcaaaag agtgtacgct tacaggctta aggaagcctt
     2101 atcctatttt attttaagaa atagtatgat aataaagtca tttttagcac gaaaaaatat
     2161 tctttgctgt ttttctactt aaaaacagtg tgtttgtgca ggggaatcat atattaataa
     2221 ttaacttgtc aaaaatatgt atttggataa tactaaatac ccttagggtt catgtttctg
     2281 caacaaagca gttagtaatt gtaatggaaa aatatacaat gttttcaaat actgattttc
     2341 attttggaca tacatcgata aatgaagata tagcatattt ttatacaaaa atgaacgatg
     2401 ctataatcaa ccttgttcgt tctcttccag tatcggttca aacctctgcg atgatatttt
     2461 taatgcataa tgctggcatt cagcctggac aaaatctgga ttttttcagc aattactatt
     2521 cgcctatatg gtcaatcatt cctcatatca tgataaggca tacatgtaca cggattaaac
     2581 aggagtttat gtatctcgct gcaacagctc atgcaatggt catgcttctt cattctttag
     2641 ataatcatat taacgatggt caggttgtac cagatcattt gactctcctt attcgaagtc
     2701 agggatggaa gctattctgg gataccattt caactttttg ttcacttatt catacagaca
     2761 aggaaatagt gaatagctgt cttgatagat attatgcgtg tataaccacg gataaaagca
     2821 ttgataatct ggaagaatat ctacgtattt tcagggatca aatggctaca gctctcattg
     2881 ttcccctgct gactgtacaa tttgtacagg atatgtcttt agacgaagat atcagagcct
     2941 tacttgaatg ttttggttta gcctggcgga tatatgatga tatccaggac atgaaggatg
     3001 atctgaataa tggacggaat tctgcagtaa ctcttagcct gcccagggaa gttcgttgtc
     3061 gctggaatga atttttaaaa ataggaacca tcatacctga aggggaaaat atatcagagc
     3121 gtaaaaatga tagcataaat attcttgaag aaatgataca gacttcaggt gtcatcccta
     3181 ttcttacaaa aaggattatt aaggaattaa aagctgctgc aaaacatgca gaagatattg
     3241 gcctttttgg cctttcagat gaaatagtga aattacgcat tcattttgtt caggggatga
     3301 aatgtgatac tggagaagac aatgtcagtg attgattcta taccatttga aattcttgaa
     3361 gctgtaaaga aggaaaacct ttcaatagaa gttacaacac gatgtaccag caactgtacc
     3421 tattgctttg tactatctga ttgtattgaa tatcatgatc ttgattatga aacagtattc
     3481 tcaataataa tggaaggtta tgagattggc tatcgacatc ttcacataac aggtggtgaa
     3541 ccgctattgt ggggatatct ttttgatatt ttgaatgatg ctatttcaat aggttatgag
     3601 gcaatttttt taaatacaaa tggattttta ttagatacta aaatattaca gcagcttaaa
     3661 tctataaagg gactgagcct ttcaatcagc ttactgggat ttgaagcaat acataatgct
     3721 ttccgtggta ataattcatt tataaaaaca acacaagcga ttgaaatggc actatacaac
     3781 aatattccag tatacctttt ctgcgtgacc gggaaaagcc ttttgccaca tgtgccacat
     3841 tttatacaat gggcatacca gaaatttcca ggtataaagg aaattactct catacagctt
     3901 attcgtgttt ctgaagatgc aggtaatgta tgtaatgagt tgttatcgcc tgatgatttt
     3961 ttatttttgg tgcgtatgat ttctttgctg aaaatatatg ggttcccggt ttcattactt
     4021 gaaaatcctc ttgctgcagt agtagctcaa ttgttccaac taccatggtt cccaaatccc
     4081 cttcacctta tacgtccggg aaaacttacc ataaaggctg ataaaagtat tacaattgcc
     4141 cattcatcaa gtcaatcggt tggcatatac agccgtggtg aactccatcg catccttgct
     4201 tctgaagttt atttacgtgg agttaaagag gatcataaca cctgttcttc ctgcaatcac
     4261 atagcagcat gtcgtaaagc aggaatgcag cgcccatcag agtttttcag ggatatgaat
     4321 gaaaatattc cctattgtaa acgtgttctt gatagtatat gttaacaagc tctggattac
     4381 tatttattat tcagtatcat aaatatcaca atcaatgtga caaaagaagg gtcagagcct
     4441 ctgagtccaa gattgctcct tttacaccca ctcaagtatc atagaatact tcttcctgta
     4501 atattcctca tactgcataa atcttgccac ccttttggca aacgtttccc cgagcttcac
     4561 aaagtagtca tgatggtcta tgggtacacc tatatttgct tcttcagtta aatatgcctg
     4621 tatgctgctg tagtggtatt ttgtagggtc agagcataac ctcctccgta tgggattata
     4681 tgccagatac cacaataacc acaacaaata atgaaagcca tctttagcaa actgtacgat
     4741 actgtccttg taccgttcat tccagaatgg cccggttctg ccggtgattt tattatacag
     4801 ttcagcaaac ctggctttta tatactgcac aatacgggat ataggagcac catcatcaag
     4861 tgtatgaatg acaagatgaa tatggttatc cataatacag aaggcaatga gcttaaagtg
     4921 atacttagct ttagctttgt ttaaaacctg tacaaagcat gccttgaaat agtcttcctg
     4981 caggtagttt ttccattcaa tgcagcggga agtgatgtga taggttaaac ctgggagttg
     5041 tacacgtaat ttccgtgcca tggtattcct ccttgtatgt agtaggtaag aaaataaagc
     5101 ctttactgta tataacgctt gtgatttgaa aattgaacgg gtatagtgaa aaatttttta
     5161 agattttgaa gattttttta tgaatcgaat agggtcagag cataataaga gataagagat
     5221 gaattacaga aacaaagggt cagagcctat taattttaag atatatcaag caatgattac
     5281 aaataatgct aagtatgaca taaatacgca acatcgacag aacaagggat ccaacatggg
     5341 gtcagagcat agtaaagata gtaaagggta aagtaaatag aatggggtca gatccttaaa
     5401 actttatatg gagaagaata ctgaatggag caggtgaatt gtagagagga ttggtaaaaa
     5461 tatggggaaa atagggggtc agagcatagt aaaagtaatc gtgcaaaagc gtaaaaaggg
     5521 gtcagagcat aaaggaccag gcaatttact tttaatggct tatatggcag actcttttgc
     5581 aggttttcaa gtgaaagatt taatagggga agttattgaa tgaaattatt aagtatatat
     5641 tttacttgaa attttgtgta taatataggg atacttcaat ataagtagca tgagtattta
     5701 attaaataga aatagaaatt agtttcatga aaagaaaata aatgactaaa atgtacaaac
     5761 gtaaagtcag ggaggttttc aatgaaagca tgtgttactg gtgcaactgg atttatagga
     5821 ggaaatttag ttaaagcatt gctggcaaaa aaacattcag taaaggcact gatattaccc
     5881 aatgatccca agataccgga actaaagaag aataaagtag aggttgtgca gggcgatatt
     5941 agggatcttg aaagtgtaaa aaaagcagta aaggggtgcg atgttatttt taactgtgct
     6001 gctgtagtga ctgactgggc accgcaaaga ctatttgaag aagttaccgt tggtggtaca
     6061 aaaaatgtgt gtgaggcttc agtgtgggct aaggtaaaaa ggcttgttg
//
LOCUS       DAOOJH010000120         5982 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_10794_length_5982_cov_9.533997, whole genome shotgun sequence.
ACCESSION   DAOOJH010000120 DAOOJH010000000
VERSION     DAOOJH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10794_length_5982_cov_9.533997"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..994
                     /locus_tag="PLJ75_11280"
     CDS             176..994
                     /locus_tag="PLJ75_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05835.1"
                     /translation="MNKKNIIRFPYLFVMLLFVMLFFIASSIAIFFQYKKVTVDINDF
                     VVEVRNISKTLITMYSDAAGLAYTTKSTYKLVSLFHQQQSLRGIKEIFFVLDNGTIVA
                     HSDETKEKELHGNITNDEFRYNIDMILYPYSIQSKQLFIYDYFMPDITIPYSKDIRHL
                     IKRFIYKDIDTNSWLVSKAVFVNGKSIGTVNCIIVKEPVYQIIDSSFYESMVIIAIIG
                     IISFFTSLIAGVIVYTRQKKVCKTLLYDIGLKLHHEGGSHIPHTGIKEAIPVKE"
     gene            1001..1582
                     /locus_tag="PLJ75_11285"
     CDS             1001..1582
                     /locus_tag="PLJ75_11285"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05836.1"
                     /translation="MVIQHRVEHSIAVVTVQGYINHTNAVEFEEYISSLTKECSSAII
                     DCSQLEYLSSTGIGALMYAERILNSIGGILVVCNCNSEVKTLLDMVSLRISIFDSVDL
                     AMQAVNTILQRLPNQNISEQATGETHEDKHYTEEKQKVVPDHGSIMFDHPIVVECPKC
                     KSMVKVNASGKFKCPECNLKFTVDDDRTIYFDV"
     gene            1678..2223
                     /locus_tag="PLJ75_11290"
     CDS             1678..2223
                     /locus_tag="PLJ75_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05837.1"
                     /translation="MKVIHVLKRIEMIDQDIRELRKLEKSLQSNKSFTQPIFITIEKQ
                     INILLGERIKLIELQIANPPANLQPEEEEIKEPLVAVPKKKGNKKKEQKMHKSKNALS
                     ELDEEAFNEQEFSMLTQDDIDKKIATMQEKIPSKLDDHDDTDVKLLDIALEKGTINKQ
                     EVETQKKKIRFFKDNFPGGES"
     gene            complement(2230..>3942)
                     /locus_tag="PLJ75_11295"
     CDS             complement(2230..>3942)
                     /locus_tag="PLJ75_11295"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HPD05838.1"
                     /translation="QLSRYLGIKDKKNIPYKVAVMFKYVHDWNWNLKVIEDMDNFDNW
                     DFQRVMHFWDTVKRFMLMSYQKISAQFNTLNLKKQMSESDFILLSRKIKSYFSVEENK
                     FEKLITFKDTPAEPILYIEPINEGIHEVKWRLFKRNRSEKNVFTTTTLRTENDLVKLL
                     VWLTVNNVYDPTITRINIQSGYVRINQNLVVELLNQMSLFFAGKEKIKNKYYLKSPFT
                     MLHCIIINFNNEAVDHLDSLYHVYSTSWGEQFIKRYTTIAHITPILQTMLQDGLELSL
                     PFDDYCLINSPGTHKRFCKNFIQLFKESYKAIVTGKTPHCRFIGELEGIYFAALRNTT
                     VEVQAFDNIVKVLTTLTLKPAVETHYSFYGDSFYLQALHTLYNKRLQRGITLGYFEEN
                     DRLQVHIINEKGNIFTFYAPKTSKNIFLYHIYGFCTNVLKRIPSSSRESQSSIICYSV
                     QTDRRGGINMVDETGKIHGDYLLNFTKSKALRVQVARHMGNEIFYNFIFPDNVSSGFM
                     TIKELYSVKHKIAELKKAGIPIYSYIGELVFNDLNQKEALLGSSIYFVEKYKLELMLQ
                     RIHI"
     assembly_gap    3943..4042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4043..4300)
                     /locus_tag="PLJ75_11300"
     CDS             complement(<4043..4300)
                     /locus_tag="PLJ75_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05839.1"
                     /translation="MDVLQTISENKDKFIAFNNEKFKRFQQLIPNPAMRRIVNTIPFL
                     LCINNKKMPGFVEGEVPLGIKNFKLDEDTMRYLHGRYPGTTF"
     gene            complement(4313..5320)
                     /locus_tag="PLJ75_11305"
     CDS             complement(4313..5320)
                     /locus_tag="PLJ75_11305"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05840.1"
                     /translation="MASLLSYNEFKATLNKHQLSHIYCFCGDEQLQKEEAVLQIASII
                     FKSKKEEDYTIATYFADDNLELALSKVLSHSLFGGTSLYVIKHCENVTANKKIHSMFR
                     DCVANIPDASYVIFDTQENKPPSLIPADSMKSIPAVQFYRPFAENCKTYIYKKCNSLK
                     LTITEEATDHLVEFTASNMKEIDDILNLISFSETANITIEFLDGFLINERRSIEFEFV
                     DTFFLKDKNALILFQEALQRNINQVLLLTLLFNHAQKIEAYHIKTQKGSTPESLMKEL
                     KIYGKRQKVFAKQSIVFNLESIRKFIAKVYVYDHKLKSYRFKGIFQENPMYDLLLQYL
                     V"
     gene            complement(5322..5903)
                     /gene="lptE"
                     /locus_tag="PLJ75_11310"
     CDS             complement(5322..5903)
                     /gene="lptE"
                     /locus_tag="PLJ75_11310"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HPD05841.1"
                     /translation="MKTKLYTIIPLLCIVVLLSCTTNEKSAVNALVHSINGQPVIPFT
                     ANKLYIEPFKNETGIQHYEDSMKIRIVEKIVANGRLAIVTSEEASDVKLMGTIQSLQI
                     QPITIDYAGIVKKKRLLIIAVISLVNTHTKTFIFRNITVQAFKEYSDIEVPITPLNIV
                     QDEVEQMLASRITLQVETGWYTALKTDAERGKK"
BASE COUNT         1927 a          957 c         1030 g         1968 t
ORIGIN      
        1 tctaaaaact ggattgtaat attgtatggg tacatgctat gctcctcagt tgtttgccat
       61 tagttgacat gctatcaaag caactttcac tatacctata aaaattgtat atatcaatat
      121 gatattttcc gatgaatact atagtatata ctggtaaccg ttattgtaaa gagttatgaa
      181 taaaaaaaac attataaggt ttccatacct atttgtgatg ctattgtttg ttatgctgtt
      241 tttcattgca agcagcattg ctattttttt tcaatacaaa aaagtaacag ttgatattaa
      301 cgattttgtt gtagaagtgc gcaatatttc caaaacactt attacaatgt actcagatgc
      361 agcaggttta gcatatacaa caaaatcaac atataagctt gtgtcattgt ttcatcagca
      421 acaatcactg aggggaataa aagaaatctt ttttgtgctg gataacggta caattgtagc
      481 acactcggat gaaacaaaag agaaagagct tcatggtaat attacaaatg atgaattcag
      541 gtataatatt gatatgatct tatatcctta ttctatacaa tcaaagcaac tttttatata
      601 tgattatttt atgcctgata taacaatccc gtacagcaaa gatatacgtc acttgataaa
      661 gcgctttatt tataaggata ttgataccaa tagctggttg gtatcgaagg ctgtttttgt
      721 taatggaaaa tcaataggga cagttaattg cattattgtc aaggaaccag tataccagat
      781 aattgatagc tcattttatg aaagtatggt gattatagct attattggta taatttcttt
      841 ttttacttca ctcattgctg gtgttattgt atacaccagg caaaaaaagg tatgcaaaac
      901 tcttttgtat gatattggat taaagttaca tcatgaaggg ggaagccata ttccccatac
      961 tggcatcaaa gaagctatac cggtaaagga gtaggctatt atggttattc aacatagggt
     1021 ggaacattcg atagcagtgg ttacagttca gggctatatt aaccatacaa atgcagtaga
     1081 atttgaagaa tatatatcat cgctgacaaa ggaatgttca tctgctatta ttgattgctc
     1141 gcagttagaa tatctcagtt caacaggaat tggagcattg atgtatgcgg aaaggatact
     1201 aaattctata gggggtatac tggtagtatg taattgcaat agtgaagtta aaacattact
     1261 tgacatggtg tcactgagaa tatcaatttt tgattcggtt gaccttgcaa tgcaagcagt
     1321 aaacactatc cttcaacggt tacccaacca gaatatctcc gaacaggcaa caggggaaac
     1381 tcatgaagac aaacactata cagaagaaaa acaaaaagtt gtaccagatc atggttcaat
     1441 catgtttgat caccctatag tagtagaatg tccaaagtgc aaatccatgg ttaaggttaa
     1501 tgcttcaggt aaatttaaat gtcctgaatg caatctcaaa tttactgtag atgatgacag
     1561 aactatatat tttgatgttt aaaaactata gcaaaataaa agtttattga cattatttta
     1621 attatagatt cactattaca tacagcagta ttaatttctt ttaattgagg tccagctatg
     1681 aaagttattc atgtgcttaa acgtatagag atgattgatc aggatattcg tgaactgcga
     1741 aaactggaaa agtcgttaca gagcaataaa tcatttacac aaccaatatt tatcacaata
     1801 gaaaagcaaa taaatatttt gttgggtgaa cgcataaagt taattgaatt gcagatagcc
     1861 aacccgccgg ctaatttaca accagaagaa gaggaaatca aggaaccgct tgttgcggta
     1921 ccaaaaaaga aaggtaataa aaagaaagaa caaaaaatgc ataaatcaaa aaatgcttta
     1981 tcagaacttg atgaagaggc attcaatgag caggaatttt ctatgcttac gcaggatgat
     2041 attgataaaa aaatagctac aatgcaggaa aaaataccgt ccaaattaga tgaccatgat
     2101 gatacggatg tcaaattact tgatattgct ctggaaaaag gaacaataaa caaacaggaa
     2161 gtggaaacac agaaaaaaaa gatacgattc tttaaggata acttcccggg tggcgaatcg
     2221 tagtttgtat taaatgtgta tacgctgtag catcaattcc aatttgtatt tttctacaaa
     2281 ataaatgctt gaccctaaca aagcttcttt ctggtttaag tcattaaata ccagttcgcc
     2341 aatatatgaa tagattggta tgcccgcctt ttttaattcg gcaatcttat gtttaacact
     2401 atataattcc ttaatggtca taaaacctga tgatacatta tcagggaaaa taaaattgta
     2461 aaatatttca tttcccatgt gacgggcaac ctgaacacgt aaagcttttg actttgtgaa
     2521 attcagcaga taatctccat gtatctttcc tgtttcatct accatgttga taccacctct
     2581 gcgatctgtt tgaacactat aacaaatgat gctggactga ctttctcttg atgaacttgg
     2641 aatacgcttg agtacattgg tacagaatcc atagatgtga tataaaaaaa tatttttact
     2701 agtctttggt gcataaaatg taaaaatatt tcctttttca ttaattatgt gtacctgtaa
     2761 tctgtcattt tcttcaaagt atcccagcgt tatgcctctt tgaagccgtt tattgtaaag
     2821 agtatgcaat gcctgaagat aaaaagaatc accataaaat gaatagtgtg tttcaacagc
     2881 gggttttaag gtaagtgtcg ttaaaacttt gacaatattg tcaaatgcct gcacctcaac
     2941 agtagtattt cgcaatgcag caaaatagat accctcaagt tcaccaataa atcggcaatg
     3001 aggcgttttg ccggtaacta tcgccttata tgattctttg aaaagctgaa tgaaattttt
     3061 acagaaacgc ttatgcgtcc caggactgtt gataagacag taatcgtcaa aagggagaga
     3121 taattccaaa ccgtcctgca gcatggtttg taaaataggg gtgatatggg ctatagtagt
     3181 ataccgcttt atgaactgct ctccccagct tgttgaataa acgtgatata acgaatcaag
     3241 gtggtcgact gcttcattgt taaaattgat tataatacag tgaagcatgg taaaaggaga
     3301 ttttaaataa tatttatttt taattttttc ttttcctgca aaaaaaagtg acatctgatt
     3361 gagaagttca acaacgagat tctggtttat acgcacataa cctgattgta tattaatacg
     3421 ggtgattgta ggatcataaa cattgttaac agttaaccat accagcaatt taacaaggtc
     3481 attttcagtg cgcaacgttg ttgtggtaaa aacatttttt tcactgcggt ttcgtttaaa
     3541 aagacgccac tttacttcat ggatgccttc attaataggt tcaatataca ggatgggttc
     3601 cgcaggtgta tctttaaatg tgatgagttt ctcaaattta ttttcctcaa cagaaaagta
     3661 tgatttgatt ttccgtgaca gtaaaataaa atctgattcg gacatctgct tttttagatt
     3721 cagggtgttg aattgagctg atattttttg atagctcatg agcataaatc gttttaccgt
     3781 gtcccagaaa tgcataactc gctggaagtc ccagttgtca aaattatcca tatcttcaat
     3841 aactttcaga ttccagttcc agtcatgaac atacttaaac atcaccgcca ctttataagg
     3901 aatattcttt ttatccttaa tgcctaaata tcttgataat tgnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nngaaggtgg tgcctggata tcttccatgc aggtaacgca
     4081 tggtatcttc gtccagcttg aaatttttta ttcccagtgg cacttcacct tctacaaaac
     4141 ccggcatttt tttgttatta atgcatagta aaaagggtat ggtattgaca attcgtcgca
     4201 ttgcgggatt ggggataagc tgctgaaacc gcttaaattt ttcattgtta aaagcaatga
     4261 atttatcttt attctcggaa atagtttgta acacgtccat acattaatag tttcacacta
     4321 aatattgcag taaaaggtca tacatagggt tttcctgaaa aataccttta aaacgataac
     4381 ttttaagttt gtgatcgtaa acatatactt ttgctataaa ttttcgtatt gattcaagat
     4441 taaatactat agattgtttt gcaaatactt tttgtctttt tccatatatc ttgagttctt
     4501 tcatcaaaga ttccggggtg cttccctttt gcgtcttgat gtgatatgct tcaattttct
     4561 gagcatgatt aaataacaaa gttaatagta acacctggtt tatgtttctt tgcaacgctt
     4621 cctgaaacag aattaatgca tttttatctt ttaaaaaaaa tgtatcaaca aattcaaact
     4681 ctattgatct tcgttcattg attaaaaatc catctaaaaa ttcaatggtt atatttgctg
     4741 tttctgaaaa tgaaataaga ttaagaatat catcaatctc tttcatattg cttgcagtaa
     4801 attctaccag atgatctgtt gcttcttcgg tgatggtgag cttgaggcta ttgcattttt
     4861 tataaatata tgttttacaa ttttcggcaa atggcctgta aaattgtacg gctggaatac
     4921 ttttcatact atcagcaggg ataagtgatg gaggtttatt ttcctgggta tcaaagataa
     4981 cataacttgc atcggggata ttggctacgc aatccctgaa catggagtgg atttttttat
     5041 tggcagtaac gttttcacag tgctttataa catacaacga agtgccgcca aaaagtgaat
     5101 gtgataaaac ttttgataat gccagttcaa ggttatcatc agcaaagtat gtagctattg
     5161 tataatcttc ctcttttttt gatttaaaga ttattgatgc aatttgtaaa actgcttctt
     5221 ctttttgaag ctgttcatca ccacaaaaac agtatatgtg cgatagttga tgtttattaa
     5281 gtgttgcttt aaactcattg tatgatagca atgatgccat actatttttt tcccctctca
     5341 gcgtctgttt tgagtgcagt ataccagcct gtttcaacct gcagagtaat gcgagaggct
     5401 aacatctgtt caacctcatc ctgaacaatg ttaagtggtg taatgggtac ttcaatatca
     5461 gagtattcct taaatgcctg taccgtaatg ttgcgaaaaa taaacgtttt agtgtgagta
     5521 tttactaaac ttatcacagc aataatcaat aatcgctttt ttttcactat tcccgcatag
     5581 tcaatggtta tgggctgtat ttgcaatgat tgtatggtac ccatgagttt tacatcgctg
     5641 gcctcttctg aagtaactat cgccaacctg ccgtttgcaa caatcttttc aacaatcctt
     5701 attttcatac tatcttcata gtgctgtatg cctgtttcat ttttaaatgg ttcaatgtac
     5761 agcttgttag cagtaaaggg aatgacaggc tgtccattga tagaatgtac taaagcgttc
     5821 actgctgact tttcatttgt tgtgcatgaa agcaaaacca caatacagag taatggtatt
     5881 atagtataca attttgtttt cataaaataa tcaagttttg aatatagatt cccgataaca
     5941 aaataagatc attttacact ccagggcctc ccactcgtac tt
//
LOCUS       DAOOJH010000121         5705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_11409_length_5705_cov_9.262478, whole genome shotgun sequence.
ACCESSION   DAOOJH010000121 DAOOJH010000000
VERSION     DAOOJH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11409_length_5705_cov_9.262478"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..558
                     /locus_tag="PLJ75_11315"
                     /pseudo
     CDS             238..558
                     /locus_tag="PLJ75_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            611..1597
                     /locus_tag="PLJ75_11320"
     CDS             611..1597
                     /locus_tag="PLJ75_11320"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD05842.1"
                     /translation="MVYTLGDVPYWGLAGVITVRLDDRVETIALARTFQAVALAITTV
                     LSPLMAHWLSFSDKTTAIGWTRTAIIVSIIGMGLFTLAFFNTTEKVPLQHNKQTIRQM
                     VNAIITNKPLLLILLGSALNFGRNIVQVGGAVVASIVFGDEKIFSLLGGVLIAAIIIS
                     NLLTPLVLRVMSKKMLMIISSLLSALTYTAMYFIGYSNFWLFVFMLFVSGFFTGFFIV
                     IQTAMIADSVDYYEYKTGSRNEGVSFAGLTFISKLMGALATMVFGMVVVAVGYSTGVV
                     ITPYIKDGIFFSITILPAISCAIGIIPFLFYPLGDSTMSEILSQLQQQRGSA"
     gene            complement(1634..4576)
                     /locus_tag="PLJ75_11325"
     CDS             complement(1634..4576)
                     /locus_tag="PLJ75_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPD05843.1"
                     /translation="MKGEWGIYKHPVLLYHLHKIKKHIYTRVADVTVSITTDSEPIPY
                     DERLNRNYRPLRKGDVWGKAYDCAWLHVKGVIPAGLASSHIVVIVTIDGEGVCYNNGM
                     EVCAINSRCTFMDYLQPTWDRRVIAIDDATSCGLLDVWIDISFNGKMVQPFGKAKFHD
                     AYIALCRDDIKDMYYEYLTYCYACLACDDTAMRHRLLSNGDAAYKALQGYLPPDVAKA
                     KAILNRAYTIVGGTSDYGVTAVGQGHLDLAWLWPVRESKRKAVRTFVNALRTIDQYPE
                     FVFGASQPQQFEWIKLNDPGLYMKIKQAVATGNIELQGGMWVECDTNLPCGESLIRQI
                     YYGKKFFYDEFGMDIVTCWLPDAFGFNANLPQILKKCGIHYFATIKLAWNEYNKFPHT
                     TFNWVGIDGSSVLAHMPPEGDYTSGATPLCVKQALDNFIEKDRLGTALLVYGNGDGGG
                     GPTQTHVELIKRQQTVQWLPRVEFGRAVDFFKRLEHYKDVLPVYKGELYLEKHQGTYT
                     TQAQNKYYNRKLELLLHECEYLAACAYLKGYSYPHALFDRIWKEVLLYQFHDILPGSS
                     VKRVYDESTMRYEAMTVELEDAIKNSIRYLSDDSHGKSCINAAPFARTEFFKVDSHWY
                     SAEIAGYSSVPLKEVVAEFPVSCGSDYIENDYLKAKFSKAGYIVSLFDKINNTEVCGD
                     YFNKLILYTDRWKFYNAWDIDSNYRKKKKIALTLVHCETFVDGPMAVRRNYYNHKKTT
                     LRQDVTVYAGKPYIEFDTECDYHETFKMLRADFMPLVFDDTVLCDIQFGHYGRSTKDG
                     TSLERAQYEICAHKWVDVSNSNYGVALINDCKYGHRVKEGVISLNLLRSPVYPDPSAD
                     RGKHVFKYALYPHTGDFKSCDTLCYGYIFNYAPRLADGTVTIPQIVCTNNNRIIIETV
                     KKAELQDAIVIRAYECYGNDTIASFDVSFNVKQAYEADMLENVLNKTNLQSVTFTPFE
                     IKTFVLQL"
     gene            complement(4738..>5705)
                     /locus_tag="PLJ75_11330"
     CDS             complement(4738..>5705)
                     /locus_tag="PLJ75_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05844.1"
                     /translation="KESEENVKQRDVIKNLLVSVQGLSQQLTALAEQSSESSAVFSNG
                     AQSQAASAEEITSTVEEMTAGIESISDSVTKQFDMITRLIVRINELSMFIEGMGTRVN
                     VSAGKAEDVSKQGSLVEGSLNTMNESMGSIMKSSQDMNNIIEIINDIADQINLLSLNA
                     AIEAARAGDAGRGFAVVADEISKLADRTTTSIKEIASLITINVKEIQSGFANIEQMTA
                     NIKKMVTSVAMISDEMKQLFESMQQQLKDNEAVRSDAQTLKNFSDSILSAIEEQKTAA
                     MEIAKSVATVNETAQGNAAGSITLAENAHELLQLAKGLQDEIAHV"
BASE COUNT         1632 a         1288 c         1001 g         1784 t
ORIGIN      
        1 aattagtata tactatttat gattcttatt gtttaatata gcactacatc aaaaaattta
       61 agtcaacaat aaaaatatat tattttttca acacaaatat tgtactattg ccagccatac
      121 ttaccaaaat gtcaggatac agttctttac ataaaaattt catgcatatc aaatatattg
      181 acaaaacgat tgtacaccat ttactattga aaactcttat cataaaagga gcaatgtatg
      241 gattatagta ccaaactatc aaataaagaa aaatattcat ttgctatagc cggttttgga
      301 caaaatatgg taataacatt tacaacaaca ttcatgttag tgtatctcta taattttgtc
      361 ggcttcagcc tatcggggat tgctaccctt actgctataa taggagctgc aaaaatatgg
      421 gatgcactca atgatctcat aatgggcatg tttgtagatt atacccatac ccgatggggt
      481 aaattacgac catatatact cctatcagct tttcctattg ctctgcttac catattgcta
      541 tttgctatcc cacaaactac tgaaacatat aagcttatct ttttttgcta ttgtttacat
      601 cttatgggat atggtctata ccctgggcga tgtcccctac tggggactgg caggagtcat
      661 aaccgtaaga ctggacgaca gagtagaaac tattgcatta gcaaggacat tccaggcagt
      721 agcactggcc attacaactg tcctcagtcc cttgatggct cactggttaa gcttttcaga
      781 taaaaccact gcaatagggt ggacacgcac agcaattata gtatcaataa taggcatggg
      841 actttttacc ctggcatttt ttaataccac cgaaaaggtt ccccttcaac acaataaaca
      901 aacaatacgg cagatggtca atgccattat taccaacaaa ccacttttgc ttatcctttt
      961 aggcagcgca ttaaactttg ggcgaaatat tgtacaggtg ggaggtgctg ttgttgcatc
     1021 gatagttttt ggtgacgaaa aaatattttc tctgttaggt ggtgtgctta ttgcagctat
     1081 tattatatca aacttactta ctcctctggt gctaagagtt atgtctaaaa agatgcttat
     1141 gattatttca agcctactgt cagcattaac atatacagcg atgtatttta ttggctatag
     1201 caacttctgg ctgtttgttt tcatgctttt tgtttcagga ttttttacag gatttttcat
     1261 tgtgatacaa acagcaatga ttgctgattc ggttgattat tatgaatata aaaccggatc
     1321 gcgcaatgaa ggggtaagtt ttgccgggtt aacatttatt tcaaagctca tgggggcact
     1381 ggcaaccatg gtttttggga tggttgttgt tgcagtaggg tattcaaccg gtgttgtcat
     1441 tacaccttat atcaaagatg gcattttctt ttccataacg atactgcctg ccatcagttg
     1501 tgctattggc attatcccat ttctcttcta tcctcttggt gatagcacaa tgagtgaaat
     1561 tctatcacaa ttgcaacaac aacgtggcag tgcataatca ggtgatgata aaaaatttat
     1621 aataatcgtt gcattatagc tgtaatacaa acgtttttat ctcaaaggga gtaaatgtaa
     1681 cagattgtaa atttgttttg ttcagtacgt tttccagcat atctgcttca tacgcctgct
     1741 taacattgaa gctcacatca aaagaagcaa tggtatcgtt gccataacat tcatacgcac
     1801 gtataacgat tgcatcttgc agttctgctt ttttaactgt ttcaataatg attctgttgt
     1861 tattggtaca aactatctgc ggtatagtaa ccgtaccatc agcaagcctt ggggcataat
     1921 taaatatata tccatagcat agagtgtcac agcttttaaa atcacctgtg tgcggataca
     1981 gtgcatactt aaagacatgc ttccccctgt ctgctgacgg gtcaggataa acaggtgagc
     2041 gcagcagatt aaggctgata acaccttctt taaccctgtg cccatactta caatcattta
     2101 ttaacgcaac tccataattg ctgttactta catcaaccca tttatgcgca caaatttcat
     2161 actgtgcccg ttcaagcgac gttccatctt ttgtacttct gccatagtgt ccaaactgaa
     2221 tatcacacaa aacagtatca tcaaacacca gaggcataaa atcagcacgc agcatcttga
     2281 acgtttcatg gtaatcacat tcggtatcaa actctatata aggttttccg gcatacactg
     2341 ttacatcctg ccgcagggtt gtttttttat ggttatagta atttctgcgc accgccatgg
     2401 gaccatctac aaatgtttca caatgaacca gcgttagcgc tatctttttc tttttgcgat
     2461 agttgctatc aatatcccat gcattgtaaa atttccatct gtcggtgtaa agaatcaact
     2521 tattgaaata atccccgcaa acctccgtat tgttaatttt gtcaaacagt gaaactatat
     2581 atcctgcttt cgaaaatttt gctttgagat agtcattctc aatatagtcg ctgccacagc
     2641 ttaccgggaa ttctgcaacc acctctttca ggggaactga actatatcct gcaatttcag
     2701 cactatacca gtgactatcg accttaaaaa actccgtgcg cgcaaatggt gctgcattaa
     2761 tacatgattt cccatggcta tcatcagaaa gataacgaat actgttcttg attgcatcct
     2821 caagctcaac cgtcatagcc tcataacgca tggtgctttc atcatatacc cgcttaacgc
     2881 ttgaaccggg taagatatca tggaactgat acaaaagaac ctctttccat attctgtcaa
     2941 atagcgcatg gggatagctg taccctttaa gataagcgca ggctgcaagg tattcacatt
     3001 catggagcag caattctaac ttccgattat agtatttgtt ttgagcctgc gtggtgtagg
     3061 tgccctgatg tttttccaga tacagctcgc ccttataaac aggaagcaca tccttatagt
     3121 gttccagccg tttaaaaaaa tcgaccgctc ttccaaactc cacccgtggc aaccattgta
     3181 ctgtttgctg gcgcttgata agctcaacgt gtgtctgcgt tgggccacct ccaccgtcgc
     3241 cattaccgta aacaagcaat gccgttccca acctgtcctt ctcaatgaaa ttatccagag
     3301 cttgctttac acacagcggt gtagcaccac tggtgtaatc gccttccggg ggcatgtgcg
     3361 ccagcacact gcttccatca ataccaaccc agttaaacgt tgtatgggga aatttgttat
     3421 attcattcca tgccagctta atagtggcaa aatagtgtat cccgcatttt tttaaaatct
     3481 gcggcagatt tgcattaaat ccaaatgcat ccggcaacca gcaggtaact atatccattc
     3541 caaattcatc ataaaaaaat tttttgccat agtaaatctg gcgtattaac gattcaccgc
     3601 aggggaggtt ggtatcacat tcaacccaca tgcctccctg taattcaata tttcctgttg
     3661 ccaccgcctg ctttattttc atgtaaaggc cgggatcatt tagttttatc cattcaaact
     3721 gttgtggttg acttgcacca aaaacaaatt ccggatattg atcaatagta cgcaaagcgt
     3781 taacaaatgt acgcaccgct ttgcgctttg attcccgtac cggccatagc catgctaaat
     3841 caagatgccc ctgcccaacc gcagtgacac cataatctga agttccacca acaatggtat
     3901 atgctctatt aagaatagct ttcgcctttg caacatccgg tggcagataa ccttgaagcg
     3961 ctttataggc cgcatcaccg tttgatagca accgatgccg catggcggtg tcatcgcagg
     4021 ctaaacaagc ataacaatag gtcagatatt catagtacat atcttttata tcatcacggc
     4081 agagggcaat atacgcatca tgaaattttg ccttgccaaa gggctggacc atcttcccgt
     4141 taaagcttat atcaatccaa acatccagca atccacagct ggtggcatca tcaattgcaa
     4201 taacccgcct gtcccatgta ggctgcagat aatccataaa cgtgcatctg ctattaatgg
     4261 cacagacttc cattccatta ttgtaacaca ccccctcacc atcaatagta acaatcacaa
     4321 caatatggct ggacgcaagc cctgcaggta taaccccttt cacatgcagc cacgcgcaat
     4381 catacgcttt cccccagaca tcaccttttc ttaatgggcg atagttcctg tttaagcgtt
     4441 catcatacgg gattggttcg ctgtcggtag taatagagac tgtaacatcc gcaactctgg
     4501 tatagatatg tttttttatc ttatgtaaat ggtaaagcaa aacaggatgt ttatagatac
     4561 cccattcacc tttcattgta ttattatata ccactcagta taggtttcat ggcgataatt
     4621 ctcatcagaa tatacaccaa caaaacaatc attgcctcat atggtaaatc ttcctgaaca
     4681 tataaccaac aaaatcacat gtgaatatat ttatagcaaa accgggcatt aatatgctta
     4741 tacatgagca atctcatcct gtaagccttt agcaagctgc aataactcgt gtgcattttc
     4801 agctaaggtt attgaaccag cagcattacc ctgcgctgtt tcattgacgg ttgccaccga
     4861 tttggcaatc tccatggctg cagtcttttg ttcctcaatt gccgataaaa tactatctga
     4921 aaagttcttc aatgtctgtg catcactgcg taccgcttca ttatctttaa gctgttgctg
     4981 catcgattca aaaagttgtt tcatttcatc agaaatcatt gccaccgatg ttaccatctt
     5041 cttgatgttt gctgtcatct gttcaatgtt ggcaaaaccg ctctgtatct ctttcacatt
     5101 aatagtaata agcgatgcta tctcctttat acttgttgtt gtcctatctg ccagcttcga
     5161 tatttcatca gctacaacag caaaccctct gccagcatcc cctgcacggg ctgcttcaat
     5221 ggcagcattg agcgacagta aatttatttg atcggcaata tcattgatta tttcaatgat
     5281 attattcata tcctgggagc ttttcatgat actgcccata ctctcattca tggtattgag
     5341 cgacccttcc accaatgatc cctgctttga tacgtcctct gctttgcctg ctgaaacatt
     5401 tacccgggtg cccatccctt caataaacat ggaaagttca ttgatcctga ctatcaaacg
     5461 ggtaatcata tcaaactgct ttgttactga atcgctgatg ctttcaatac cggcagtcat
     5521 ttcctctacg gttgatgtta tctcttctgc ggaagctgcc tgtgattgtg ctccgtttga
     5581 aaatacagca gaagattcag aactttgctc cgcaagtgca gtcaactgct gtgacaaacc
     5641 ctgtaccgat accagtaaat tttttataac atcacgttgc ttaacatttt cttcagattc
     5701 ttttt
//
LOCUS       DAOOJH010000122         5699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_11420_length_5699_cov_8.968462, whole genome shotgun sequence.
ACCESSION   DAOOJH010000122 DAOOJH010000000
VERSION     DAOOJH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11420_length_5699_cov_8.968462"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..434
                     /locus_tag="PLJ75_11335"
     CDS             6..434
                     /locus_tag="PLJ75_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPD05845.1"
                     /translation="MRLSTRSRYGVRMMFEFALNYDKGPVYLKDVAAIQGISFKYLSK
                     LVIPLKSAKLLKSVRGVHGGYMLAQRPEDITIRKIVQILEGDVSLVECAENPEACSRY
                     AECVARSIWEKADKAIMDVLNSISLQDMMNEYKKNFFNSV"
     gene            460..1377
                     /gene="cysK"
                     /locus_tag="PLJ75_11340"
     CDS             460..1377
                     /gene="cysK"
                     /locus_tag="PLJ75_11340"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011746137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPD05846.1"
                     /translation="MKIYNSVTELIGNTPLVKLSGLAPEGVTIMAKLESANPLSSVKD
                     RIGFAMIRDAEEKGLLHDKTIIVEPTSGNTGIALAFVSAVKGYKLMLTMPDTMSMERR
                     KLLQMLGAEIVLTDGAMGMRGAIEKAQEIVERTTSAFMPQQFNNPANPEIHRKTTAME
                     IWNDTGGNVDVFVAGVGTGGTITGVGEVLKAKKPSVTIIAVEPEKSPVLSGGKPGPHK
                     IQGIGAGFIPQVLNRSIIDEIVTVDDTDAGNTASKLAKVEGILAGISAGAAMWAACKI
                     ASRPESKGKTIVVLLPDSGERYVSTWLFD"
     gene            1608..2891
                     /locus_tag="PLJ75_11345"
     CDS             1608..2891
                     /locus_tag="PLJ75_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HPD05847.1"
                     /translation="MSKNYRIETIALHGGQSLDATRSRAVPVYRTSSYVFDSAKHGAD
                     LFSLKEKGYIYTRMMNPTQDVLEQRVALLEGGSAALALASGTSAIFYSIINICKAGDE
                     FISAGNLYGGTYTMFDAILPQFGITCRFVSYDDYGAYEKAVNEKTRAIFVESIGNPTL
                     GVPDFETIASIAHKHHLPVIVDNTFATPYHFKPIEYGAAVVVHSLTKWLGGHGTAIGG
                     IVVDAGNFDWTDPRFSLYNEPDDGYHGLQFAHDLGDDNALAFILRMRLVSLRNLGAAI
                     SPDNAWLFLQGIETFALRMQRHSDNALKVAQFLQKHSKVAWVRYPGLAGDSCYDNASK
                     YFNNGFGGMVVFGVKSGYAGAVKFINSITLFSHLANVGDAKSLILHPASTSHSQLSEQ
                     QLLASEVTPDLIRLSIGIENIEDIIEALDMALKLC"
     gene            3079..3936
                     /locus_tag="PLJ75_11350"
     CDS             3079..3936
                     /locus_tag="PLJ75_11350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPD05848.1"
                     /translation="MLKKSIIIIVAIIVVGILLLGYVFYRATTIPSNNVDAMLKGTIA
                     PDKKIVICFGDSITHGAVSFDYVRFLAEDAALKKFIFVNEGVNSRLVYNLLQVVDKVA
                     AVKPHYVFILIGTNDLKGSLSDDEYNRYNKLWKLPQRPTKQWFADNYKKLVDILKAKT
                     DATIVLISIPPLGENCDSVPFTLAMEYSKIIKQIAAEKKVLYIPFNEILSTELIQHGK
                     KDIPPYTFNEWFMYTSIVQHYLCWRSWNAIADRRGLLFTPDNIHCNERSGMMLTETIK
                     NILLSSNEH"
     gene            complement(4104..4316)
                     /locus_tag="PLJ75_11355"
     CDS             complement(4104..4316)
                     /locus_tag="PLJ75_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05849.1"
                     /translation="MAADYYSQYHELCNFFTGTIAMKILKIFGLCLLIVIVTTALIIA
                     LWFIRPNTSEVKHSLALEIWDAVNDG"
     gene            4536..>5699
                     /locus_tag="PLJ75_11360"
     CDS             4536..>5699
                     /locus_tag="PLJ75_11360"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPD05850.1"
                     /translation="MKDVVIASIVRTPVGTFGGALKDVSAVDLGVIVVREAVKRAGLS
                     PQQVENVILGNVLQAGLGQNTARQVLIHAGMPHEVPAMTINKVCASGLRSISMAAQII
                     KAGDAEIIVAGGIENMSASPFALPKARWGYRMGDDKIVDIMIKDGLWDAFNQYHMGIT
                     AENVAERWKLTREELDQFSLASQTKAEAAIKSGRFKDEIVTVVIKDKKGEKIFDTDEH
                     PRFGTTLEALSKLKPSFKKDGMVTAGNASGINDGAAAAVVMSADKAKELGIKPLCRIA
                     SYASAGVDPAIMGTGPIPASRKALAKAGWKVQDLDLIEANEAFASQAVVVNKEMGWDI
                     SRVNVNGGAIAIGHPIGASGARILTTLIHEMIKRDARKGIATLCIGGGQGCAVT"
BASE COUNT         1750 a          982 c         1345 g         1622 t
ORIGIN      
        1 atgctatgcg actatcaaca cggagcagat atggtgtgcg aatgatgttt gaatttgcac
       61 tgaactatga taaaggacct gtgtatttaa aagatgtggc agctattcag ggtatttcgt
      121 ttaaatattt aagtaagctg gttatcccgt taaagtctgc aaagctgctt aaatcagtgc
      181 gaggggtgca cggtggatat atgctggcgc aacgtcctga agatatcacc atacgcaaaa
      241 ttgttcaaat acttgagggc gatgtttcac ttgttgaatg tgctgaaaat ccggaggcat
      301 gcagcaggta tgctgaatgt gttgctcgca gtatatggga aaaggcggat aaagccatta
      361 tggatgtgct caacagtatt tcgttgcagg atatgatgaa tgaatataaa aagaattttt
      421 ttaacagtgt atgaaatcta atttagtacg agggtagtaa tgaagatata taactcagta
      481 acggaactca ttggcaatac accattggtt aaattgtcag ggttagctcc ggaaggggtt
      541 accatcatgg caaagcttga atcagctaat cccctgtcaa gtgtcaaaga cagaattggt
      601 tttgccatga ttcgcgacgc tgaagaaaag ggattgttgc atgataaaac aattatagta
      661 gagccgacaa gcggcaatac aggcatagcg cttgcatttg tttctgcggt aaaaggatac
      721 aagcttatgc tgaccatgcc cgataccatg agcatggagc gtcgcaaatt attacagatg
      781 ctgggcgcag agattgtcct tactgatggt gccatgggga tgcgtggcgc catagaaaag
      841 gcgcaggaga ttgttgaacg cactacatct gcttttatgc cacaacaatt taataatcct
      901 gcaaatcctg aaatacatag aaaaacaact gccatggaaa tatggaatga tactggcggc
      961 aatgttgatg tatttgttgc aggtgttggt acagggggaa ccattactgg tgtaggcgaa
     1021 gtactaaaag ctaaaaaacc atcagttacc attatagccg ttgaaccgga aaaatccccg
     1081 gtgctttccg gaggcaaacc cggtccgcat aaaattcagg gtattggtgc agggtttatc
     1141 cctcaggtac tcaaccgctc tattattgat gagatagtta ctgttgatga tactgatgca
     1201 gggaatactg ccagcaaatt agcaaaggtt gagggaatac tggctggtat ttcagcaggg
     1261 gctgctatgt gggcagcttg taaaatagcc agccgtcctg aaagcaaagg caagactata
     1321 gtagtgctcc tgccagattc cggtgagcga tatgtaagca catggctatt tgattgatgg
     1381 aggccattgc aggaagagtt gcccatgggc agcttacagc cacatataat tgatgaaaat
     1441 ggtctggttt aagtcatcct gaaatgtaga aaatcatcct ggattcattt caggatctca
     1501 gttacaaatt ttgattttgg gccaagcacg gaatgacagg gcgtatagtt ctatttttgg
     1561 agtagaaatt tatcttcaat gatgaaataa tgtatgaggg ggatataatg tcaaaaaact
     1621 atcgcataga aacaattgcc cttcatgggg gtcagagcct tgatgcaaca aggtctcggg
     1681 cggtgccggt atatcgtacc agctcatatg tatttgattc agcaaaacac ggtgcagatc
     1741 ttttttcact caaagaaaaa ggctatatct atacccgaat gatgaatcca actcaggatg
     1801 tccttgaaca gcgggttgca ttgttagaag gcggtagtgc ggcattagca ttagcttcag
     1861 ggacatcggc aatattttac agcattatca atatatgtaa agcaggtgat gagttcataa
     1921 gtgcaggcaa cctgtatggt ggcacctata ccatgtttga tgcaattctg ccacagtttg
     1981 gcattacatg ccgttttgtt tcgtatgatg attatggtgc ctatgaaaaa gccgttaatg
     2041 aaaaaacacg ggcaatattt gttgaatcaa taggtaatcc aaccttaggg gttcctgatt
     2101 ttgaaacgat agcatccatt gcgcacaaac atcatcttcc ggtgattgtt gataatacct
     2161 ttgcaacccc gtatcatttc aaacccattg aatatggcgc tgccgttgtt gttcattcgc
     2221 ttaccaagtg gctgggaggg catggcaccg ccattggtgg cattgtggtt gatgcgggta
     2281 actttgattg gactgatccc agattttctt tgtataatga gcctgatgat ggctatcatg
     2341 gattacaatt tgcacacgat cttggcgatg acaatgctct tgcatttatt cttagaatgc
     2401 ggctggtatc gcttcgcaat ttaggtgctg ccatctctcc tgataacgca tggctatttt
     2461 tgcagggaat tgaaacattt gcactgcgca tgcagcgtca tagcgataat gctttgaagg
     2521 tagctcaatt tttgcaaaaa cattcaaagg tggcatgggt acgctatcca gggctggctg
     2581 gcgatagttg ttatgataat gcttcaaagt attttaacaa tggatttggc ggtatggtgg
     2641 tttttggtgt caaaagcgga tacgccggtg cagtaaagtt tatcaattcc attacgcttt
     2701 tttcgcatct ggcaaatgtt ggcgatgcaa agagtttgat attgcatccc gcaagcacat
     2761 cccattcgca gttgtctgag cagcagctgc tagcatcaga ggtgaccccc gatcttatcc
     2821 gcctttccat cggaattgaa aatatagaag atatcattga agctcttgat atggctttga
     2881 agctgtgttg aaggaatagt ggagcgatac cgcgattgat tactattgtg tatcagactg
     2941 catcaggata attacacaac catatatagt ttgtgactct ttaaaaaatc atttgcaaat
     3001 atgttgttat aggcttatta tgataaggta acctatcatc agtcatggtt tactacatgc
     3061 atcatgagga ggaaaagtat gctcaagaag tcaatcatca tcatagtggc aatcattgtt
     3121 gttggtatac tgctgctggg atatgtcttt tatagggcaa ctacaatacc ttcaaataat
     3181 gttgatgcca tgctcaaggg aactatcgcc ccggataaaa aaattgttat ttgttttggc
     3241 gatagtatta ctcatggggc tgttagtttt gattatgtaa ggtttttagc ggaagatgct
     3301 gcattgaaga aatttatttt tgtcaatgag ggtgttaaca gcaggctggt gtacaacctt
     3361 ttacaggttg ttgataaagt agcagcagta aagccgcact atgtatttat tttaattgga
     3421 acaaacgatc tgaagggatc gctttcggat gatgaatata ataggtacaa taaattatgg
     3481 aagcttcccc agaggccaac aaagcagtgg tttgccgaca attataaaaa actggttgat
     3541 atcctgaaag caaaaactga tgctactata gtgcttatct ccataccgcc attgggtgaa
     3601 aattgtgaca gtgttccctt tacgctggcg atggagtact caaagattat aaaacagata
     3661 gcagcagaaa aaaaagtgct ctatataccg ttcaatgaaa tcctttccac tgaacttata
     3721 caacatggca aaaaggatat accaccatac acattcaatg aatggtttat gtatacatca
     3781 attgtgcaac attatctttg ttggcgtagc tggaatgcta tcgcagatag aagaggtttg
     3841 ctgtttaccc ctgacaatat ccattgcaat gaacgaagcg gtatgatgct tacagaaaca
     3901 ataaaaaata ttttactatc cagcaacgaa cattaataac attaatgttt attgctattg
     3961 atatgcttaa gattgcagag gcgttatact acgcttttgc atgaatgtac cctctctttg
     4021 ataaatatgt gtacacaaaa aatagagaga ggattgtagt cattaatagt aaaaatatac
     4081 accgttagaa tgtatggcta aaatcaacca tcattgacgg catcccatat ttcgagtgca
     4141 agtgaatgtt taacctctga agtattggga cgtataaacc ataatgctat gatgagtgct
     4201 gttgtgacaa taacaatcaa caaacaaaga ccaaaaattt taagtatttt catggcgata
     4261 gttcctgtaa aaaaattgca caactcatgg tactgagaat agtaatcggc agccatgacc
     4321 tgctatcatg caagtactca aacactacgg tatagatgca gtaagaaata cgatacgtga
     4381 gtacttactc gatacgcata cttacgtatt gtcaataaat attatttttt ctttacaaca
     4441 tggcaacagt ttattagtat tacatcaatt gtacagattg ttgtaataat tttataataa
     4501 ttaatatgaa aaatttttaa taatgagagg tctttatgaa ggatgttgta atagcgtcaa
     4561 tagtgcgaac accagtaggt acttttggcg gagcattgaa ggatgtttcg gcagttgatc
     4621 tgggggttat tgtagtcagg gaagctgtaa aacgagccgg gctgtcacct caacaggtag
     4681 agaatgttat actgggaaat gtgttgcagg caggtttggg acagaataca gccaggcagg
     4741 tattgatcca tgctggtatg cctcatgaag tgccggctat gactatcaat aaggtatgcg
     4801 cttccgggct tagatccatc agtatggctg ctcagattat taaagcaggc gatgcagaaa
     4861 ttattgttgc cggtgggata gaaaatatga gcgcttcccc gtttgcttta ccaaaagcac
     4921 ggtggggata tagaatgggt gacgataaaa tagttgatat catgataaaa gatggtttgt
     4981 gggatgcatt taatcaatat catatgggaa taacagcaga aaatgtggct gagcgctgga
     5041 agctgactcg tgaggaatta gatcaatttt cactggcaag ccagacaaag gcagaggctg
     5101 ctattaaatc aggaaggttt aaagatgaga tagtcactgt tgtaataaag gataaaaaag
     5161 gagaaaaaat ttttgatact gatgaacatc cccgctttgg aacaacactg gaagcattat
     5221 caaaattaaa gccatcattt aaaaaggatg gtatggtaac cgcagggaac gcaagcggga
     5281 ttaacgatgg cgcagcggct gcagtagtca tgtcagcaga taaagcaaaa gagctgggga
     5341 ttaagccttt atgccgtatt gcatcgtacg cgtcagcagg tgttgatcct gcaatcatgg
     5401 ggaccggtcc aatacctgca agcaggaaag ctcttgcaaa agcaggatgg aaagtgcagg
     5461 atttagattt aattgaagca aatgaggctt ttgcatctca ggctgtggtt gttaataaag
     5521 agatgggatg ggatatatca agagtcaatg ttaatggagg agctatcgca ataggacatc
     5581 ccattggtgc atcgggtgca cgaatattaa caacactgat tcatgaaatg ataaaacgtg
     5641 atgccagaaa aggcatagca acattatgta taggtggcgg gcagggttgt gcagtaaca
//
LOCUS       DAOOJH010000123         5672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_11464_length_5672_cov_6.073705, whole genome shotgun sequence.
ACCESSION   DAOOJH010000123 DAOOJH010000000
VERSION     DAOOJH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5672
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11464_length_5672_cov_6.073705"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLJ75_11365"
     CDS             complement(<1..1084)
                     /locus_tag="PLJ75_11365"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPD05851.1"
                     /translation="MHIKKFFLSMLITCGIGVIISAYLLYIHYFPLIPDAALYSLCTA
                     GSTFDCNAVNTSAYAMLFGMPLAAWGMAFYLFFIAVILIYTIKTKHVILEFGMLWLAI
                     FAVLLSVVLGYISFVKIQKFCSFCMALWLCNVALLTLVLIYIALLYKGLIRGINTIHD
                     FTVATLFADSKLRGTIVVLVIAAVVSLLVAWGIDGTLQLAYNQKQKQREAIILQEFKK
                     EYTMFERVAIPVDGTIPVIGKKEYPVHITVFFDFNCGACHRAIAVLSELAKKYEGKVA
                     VYLRHFPLDATCNRFIQHKKDGASCMASGIAYALYGSNEYHDYIMQLMSYRGKVDEIA
                     INRVVSDLHKDYADLKKLSNTDTIKKL"
     gene            complement(1102..2127)
                     /locus_tag="PLJ75_11370"
     CDS             complement(1102..2127)
                     /locus_tag="PLJ75_11370"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPD05852.1"
                     /translation="MQHGKLYSIMLCVLLVTGCSDKVTVTECGIFAPSRLHYVKGQDG
                     VTPVDFDAQHTMWTFGDTITHEGMIANSLAFTNKVDASNIRELPFHYYTENGEIVPFI
                     RNDKHEDPSRDRLWAFDGIRINNTVYVYYVHVYIHDPLKPLSFSLMESSVAHWDVPYN
                     WSLGKPVRFKRFKNLFTGDVPALGASVLQHNDYVYVVGHISKNGKSSLVIARVKPQHI
                     LQKDTYAFLQGDSSWKNTVTGAIHLYGDVAGECSLGYDEDYKFFYIVYCQLFNGNIVM
                     SIAPSIEQLPFAKKIILYTPKKLKGTSMMYYSAKEILHMKNQWYIIYINPAEYQPYFI
                     QVDINVH"
     gene            2279..>5672
                     /locus_tag="PLJ75_11375"
     CDS             2279..>5672
                     /locus_tag="PLJ75_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPD05853.1"
                     /translation="MELKYGVTVWGNYFLEAIYQYYADIKRLQRGLRYANSDRVKKIE
                     INNTLVLAKVKGNQGRSYTVSLYFSPFKSDEKEKVVTLIEDNLLVLGEIIKGNLPDIL
                     LDELTKNNIKLFPSDWKELNRGCSCPDWGDPCKHMAAVYYLLTREIDLNPFLLFTLRG
                     LDLTKLFKITTKSLIDYPINIQEAHENQYNNELQSFREVAIVTINNYTNFILSKLSNN
                     PSFIDKDFKPIIEKFYKKSQALSDAILLRDIASMDSDLLLKLENLFKSGKISFIPDKN
                     FVGSYFTFYSELIEHEPVRQKIDSLNIMVYDAKTSTYHISPLNMLKLFLQFTTMYESN
                     ESTSECLMYNFLFILSRVAYNFVKSNAFIPAVYLEKNQFYCIFRLLSSPVDIKKQIHN
                     VISAMPVSLTLPSLTGKNTKKSRGTMDKESTIKFMLACFFTEYASIIGTSMKSSLKLT
                     KQFHSTMYDAIFLSKPYSMERFNDKALPLAIANYFSIFELIESNLDFSVIIDKHNDEY
                     FLQFLVNNTTINQITEQKTKNIVLKVLSNFIDVLPEIPLLLKNSTLPVSQERLEDLIT
                     KKLELLQNLGVEVLLPKELKEIIKPQLKIKTRLKDKTHVSFFSLNDMVTFDYEFYIDN
                     ETISYEELIALMNQNKELLKFRDSYIRINTTDIAKLMQQYETKSNLSQFDLLKSHLSN
                     ESETIELCDEIKAMFETMFTEQQYQLPHLNASLREYQERGFNWMLHLLLHGFGVILAD
                     DMGLGKTLQAIAVIQFLKENGYIKKPMLIVVPKTLMFNWESEIKKFSENLSYTFYYGT
                     KRTFSDTDIIFTTYETLQRDYKKLEDIVYDGIILDEAQKIKNYQTLTAKAIKHLSKKA
                     KFRIALSGTPIENRLTELWSIFDFILPGYLKSIDYFVNEYTKEIELKRNQQKIENLRK
                     ITSPFILRRLKTDKSIIKDLPEKIIIDQFVEMEKEQIALYEHVVQQLREEIAASENLL
                     PIILKSFTILKQICNHPRNYDKKSPAISHISGKTKLLLEILPAIIAKQEKVLIFTQFV
                     EMGNILKQIIENELLSEPLFLTGNLSQIKRKELIEKFEHDDSAQIFILSLKAGGVGLN
                     LTKANHVIHYDLWYNPSVENQATDRAFRIGQKKNVFVHRFVTK"
BASE COUNT         2012 a         1021 c          907 g         1732 t
ORIGIN      
        1 aaagcttttt tattgtatca gtgttcgata gtttttttaa atcagcataa tctttatgta
       61 aatctgaaac caccctgtta atagctattt catcaacttt cccccggtat gacattaact
      121 gcatgatata atcatgatac tcattgcttc catataaagc atatgcaata cctgatgcca
      181 tgcatgatgc accatccttt ttatgctgaa taaacctgtt acaggtagca tccagaggaa
      241 aatgccgtaa gtaaactgcc accttccctt catatttttt tgccagttca gagagcacag
      301 caattgctct atgacaggca ccgcagttaa aatcaaaaaa aacagttata tgtacaggat
      361 attctttttt acctataaca gggatggtgc catcaactgg tatggcaacc ctttcaaaca
      421 tggtgtattc ttttttaaac tcctgtagta ttattgcttc gcgctgtttt tgtttctggt
      481 tatatgcaag ctgtaatgtt ccatcaatac cccatgcaac cagaagcgac acaaccgctg
      541 caataacaag tacaacgatg gtaccacgta gcttgctgtc ggcaaacagg gttgcaacag
      601 taaaatcatg gattgtatta atacccctga ttaaaccttt atataacaat gctatatata
      661 tgagcaccag cgttaacagt gcaacattac atagccatag tgccatacaa aatgaacaaa
      721 atttttgtat ttttacaaat gatatatatc ctaatacaac tgacagcaat acagcaaaaa
      781 tagctaacca caacatacca aattcaagga ttacatgctt tgtttttatt gtgtaaatta
      841 gtatcacagc aatgaaaaat aaataaaatg ccatacccca tgcagctagc ggcattccaa
      901 aaagcatggc ataggcgctg gtattaactg cattgcaatc aaaggtacta ccagcagtac
      961 ataacgaata aagcgctgca tcaggtataa gcggaaaata atgaatatac aacaaataag
     1021 cagagataat gaccccaata ccacacgtga tgagcattga taaaaaaaac tttttgatat
     1081 gcatggaagt ttccttaaaa tttaatgtac atttatatca acctgtataa aatacggttg
     1141 atattctgcc gggtttatat agattatata ccattgattt ttcatatgca atatttcttt
     1201 tgccgaataa tacatcatag aagtaccttt taattttttt ggcgtataga gaattatttt
     1261 ttttgcaaag ggcaattgtt ctatggaggg agcaatgctc atcacgatat tgccattgaa
     1321 aagctgacaa tacacaatgt aaaaaaattt atagtcttca tcatatccaa gcgagcactc
     1381 gcctgcaaca tcgccgtaaa gatgaattgc accggttaca gtatttttcc aggaactatc
     1441 gccctgtaaa aatgcatacg tatccttttg caaaatgtgt tggggtttaa cccgtgcaat
     1501 aacaagtgaa cttttaccgt ttttgcttat atgcccaacc acatacacat aatcattatg
     1561 ctgcagcaca gaagccccca aagcaggtac atcccctgta aaaagatttt tgaacctttt
     1621 aaagcgtaca ggttttccca atgaccagtt atagggcaca tcccagtgag caaccgagct
     1681 ttccatcaat gaaaatgaca gaggtttcaa cggatcatgg atatagacat ggacatagta
     1741 tacatacacc gtgttattaa tccgtatacc atcaaaagcc cacaatctgt ctcttgaagg
     1801 gtcctcatgt ttgtcattcc ggataaatgg aacaatttcc ccattttcag tgtaatagtg
     1861 gaatggtaat tccctgatat tgcttgcatc aactttattg gtaaatgcca gactattagc
     1921 tatcatcccc tcatgagtaa tggtgtcacc gaatgtccac atggtatgct gtgcgtcaaa
     1981 atctactggc gttacaccat cctgcccttt aacatagtga agacgtgaag gagcaaagat
     2041 accgcattca gttactgtaa ccttatctga acaaccagta acgagtaaaa cacataacat
     2101 aatgctataa agtttaccat gttgcatact gtatgattag cctttacctt ttttactatc
     2161 gtatgactat cgataaatac tacttttttt caattatttt ttcttttttt gttgattaaa
     2221 cggctttatc agagctatta gtaattttta ttataatctt tttttataga gcatggctat
     2281 ggaattgaaa tatggcgtaa cggtatgggg taattatttt ttagaggcta tctaccagta
     2341 ctatgctgac ataaaacggt tacagcgggg attgcgatat gcaaatagcg acagagtaaa
     2401 gaagattgag attaacaata cccttgtttt agcaaaagta aaaggaaatc aaggcagatc
     2461 atatacagtg tcgctctatt ttagcccttt caaaagcgac gagaaagaaa aggttgttac
     2521 gctcatagaa gacaatctac ttgtattggg cgagatcatc aaaggcaatc tacccgatat
     2581 actgcttgat gaactaacaa aaaataatat taaattattc ccttctgact ggaaggaatt
     2641 gaatagagga tgcagctgcc ctgactgggg cgacccgtgc aagcacatgg cagcagtata
     2701 ctatctatta accagagaaa tagatttaaa tccattttta ttatttacat tacgtggatt
     2761 ggatttaaca aaactgttca aaatcaccac taaatcgctc attgattatc ccatcaatat
     2821 tcaagaagca cacgaaaacc aatacaacaa cgaattgcaa agctttcgcg aagtggcgat
     2881 agttactatt aataattaca ctaattttat attatcaaaa ctatcaaata atccatcatt
     2941 tattgataaa gattttaaac ccattattga aaaattttat aaaaaatcac aagccttaag
     3001 cgatgctatt cttttgaggg atatagcatc gatggacagt gatttattgt tgaagctcga
     3061 aaatcttttt aaatccggga agatctcttt tataccggat aaaaattttg ttggttcata
     3121 ttttaccttc tatagtgagc tcattgaaca tgaaccggtg cgtcaaaaaa tagatagcct
     3181 caacataatg gtatatgatg ccaaaaccag cacataccat atatcaccat taaacatgct
     3241 caaattattt cttcaattca caaccatgta tgaatcaaac gaatctacta gcgaatgttt
     3301 gatgtataat tttttgttta tcctttcgcg tgttgcatac aattttgtaa aatcaaatgc
     3361 ctttatccct gctgtttatc tagaaaaaaa tcagttctat tgtatctttc gattgctttc
     3421 atcgcctgtt gatattaaaa agcagataca taatgtaata tcagccatgc ctgtctcact
     3481 aacattgcca tcattgaccg gtaaaaatac aaagaaaagc cgtggtacca tggataaaga
     3541 atccactatc aaatttatgc tggcatgttt ctttactgaa tacgcttcaa taattggaac
     3601 atcaatgaaa tcttcgttaa agttaacaaa gcaatttcac agtaccatgt acgatgctat
     3661 atttttatca aaaccatata gtatggaacg cttcaatgat aaagctttac cattggctat
     3721 tgctaattat ttttctatct ttgaacttat agaatcaaat cttgattttt ctgttatcat
     3781 agataaacat aatgatgaat actttttgca atttttagtc aacaatacaa ccatcaatca
     3841 aattaccgaa caaaagacaa aaaatatagt actgaaagtt ttatcaaact tcattgatgt
     3901 tttacctgaa ataccgttac tattaaaaaa tagcactctg cctgtatcac aggagcgatt
     3961 ggaagatctc ataacaaaaa aacttgaact gttacaaaac cttggggtag aggttttact
     4021 accaaaagaa ttaaaagaga ttataaaacc tcaacttaaa ataaaaacgc gcttaaaaga
     4081 caaaacacat gtatcgtttt ttagccttaa cgatatggtt acatttgatt acgagtttta
     4141 tatagacaat gaaacaattt cctatgagga acttattgct ttaatgaacc aaaataagga
     4201 gctacttaaa tttagagata gctatatacg gattaacaca actgatattg ccaagctcat
     4261 gcaacagtat gaaaccaaat caaacctgtc gcaatttgat cttttaaaat ctcatctcag
     4321 caatgaatca gaaacgattg aactgtgtga tgaaatcaaa gcaatgtttg aaaccatgtt
     4381 cacagagcag cagtaccagc ttcctcatct caacgcatcg ctgagagaat atcaagaacg
     4441 aggctttaac tggatgctcc atctgctttt acatggattt ggagtaattt tagccgacga
     4501 catgggtttg ggaaaaacat tacaggcaat agctgttata caatttttaa aagaaaatgg
     4561 gtacataaaa aaaccaatgc ttattgttgt tcccaaaaca ttaatgttta actgggaatc
     4621 agaaattaaa aaattttctg aaaatctttc gtatacattt tattatggaa ctaaaagaac
     4681 attcagtgat actgatatca tatttaccac ctacgaaaca ttacagaggg attataaaaa
     4741 actggaggat attgtatatg atggcataat ccttgatgag gcacagaaga taaaaaatta
     4801 tcaaacatta actgcaaaag ctattaagca tctatcaaag aaagcaaagt ttcgtattgc
     4861 attgagtggc acacctatag aaaataggct tacagagctc tggagtatct ttgattttat
     4921 attgccaggg tatttaaaat caatagatta ttttgttaac gaatacacaa aggaaataga
     4981 attaaagcgt aatcagcaaa aaattgaaaa tttacgaaag attacatcac cgtttatttt
     5041 gcgcaggctt aaaactgaca aatcaatcat caaagattta cccgaaaaga taatcattga
     5101 tcaatttgtt gaaatggaaa aagagcaaat tgcactctat gaacatgttg ttcagcaact
     5161 cagagaagag attgccgcaa gcgaaaatct gcttcccatt atattgaaaa gctttacaat
     5221 attaaagcaa atatgcaatc atccccgaaa ttatgataaa aaaagtcctg ctatttcaca
     5281 tatctcaggg aaaacaaaat tattgcttga gattttacca gcaattatag cgaaacaaga
     5341 aaaggtatta atcttcaccc aatttgtaga aatgggaaat atattaaaac aaattattga
     5401 aaatgaatta ttatcggaac cattattttt aaccggcaat ttatctcaga ttaaaagaaa
     5461 agaactcatt gaaaaatttg aacatgatga cagcgcacaa atctttatac tttcattaaa
     5521 agcaggaggg gttggtttaa atttaacaaa agcaaatcat gttatccatt atgatctttg
     5581 gtataaccca tctgttgaaa accaggctac cgatagagcc tttagaattg ggcagaaaaa
     5641 aaatgtcttt gtacacaggt ttgttactaa aa
//
LOCUS       DAOOJH010000124         5585 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_11698_length_5585_cov_9.803797, whole genome shotgun sequence.
ACCESSION   DAOOJH010000124 DAOOJH010000000
VERSION     DAOOJH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11698_length_5585_cov_9.803797"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /gene="groL"
                     /locus_tag="PLJ75_11380"
     CDS             complement(<1..1541)
                     /gene="groL"
                     /locus_tag="PLJ75_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625622.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPD05854.1"
                     /translation="MAKILQYNDEARRSIYQGVIKLSDAVKVTLGPRGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDPFENMGAQLVKEVATKTNDVAGDGTTTATILAAAIYHES
                     LKNVTAGANPMSLKRGIDKAVEVAVKFIGDTAREIKDKKEIAQVAAISANNDMEIGEL
                     IAEAMDKVGKDGVITVEEAKSIETHLEVVEGMQFDRGYISPYMVTDPENMEAVLEDAF
                     ILINEKKISTMKDLLPILEKVAQAGKPLLIIAEDVEGEALATIVVNTLRKVIQCVAVK
                     APGFGDRRKAMLEDIAILTKGQVISEDLGLKLENTTMDMLGRAKKVIIDKENTTIIEG
                     AGSQADVQARIKVLKKQIEETTSDYDREKLQERLAKLAGGVAVINVGAATEVELKEKK
                     ARVEDALSATRAAVEEGIVPGGGLTLLYAQKAVAELEKKLDGDEKIGVRIVAKAMEEP
                     MKMIAFNAGVEGSVVVEKAKQEKQGIGFNAATLEWEDLTKAGIIDPAKVVRSALQNAA
                     SVAGML"
     gene            complement(1575..1859)
                     /gene="groES"
                     /locus_tag="PLJ75_11385"
     CDS             complement(1575..1859)
                     /gene="groES"
                     /locus_tag="PLJ75_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPD05855.1"
                     /translation="MGIKPLGDRVLIEVTEEELPKQGSLYIPDTAKEKPQQGKVLAVG
                     KGRYEDGKWVPLDVKTGDTVLFGKYAGTEVKYGGKEYLIVRENDILAILD"
     gene            complement(2408..3121)
                     /gene="pssA"
                     /locus_tag="PLJ75_11390"
     CDS             complement(2408..3121)
                     /gene="pssA"
                     /locus_tag="PLJ75_11390"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPD05856.1"
                     /translation="MSVAWIPNTLTLGNLVLGFIAIIFASMNIPHHLSIAGVLILGAA
                     LLDGLDGQVARMLGVASKLGQELDSLADCVTFGIAPGYLAFKGYLQGTWIYILDKPID
                     IGIFIAAIFPVCAAYRLARFNVQHVPNAFDGLPSPIAGIIVALVPLCFINIIIPKLLF
                     IIGFVLVALLMVSTVRYAKPQSFILQKITGIKLLLFILILIALIFMFKQWVIMVIIAL
                     YILSGLLSFIIQLIQDYRY"
     gene            complement(3221..3904)
                     /locus_tag="PLJ75_11395"
     CDS             complement(3221..3904)
                     /locus_tag="PLJ75_11395"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPD05857.1"
                     /translation="MNYRTKKIKGLYVDTVLFDMDGVIVDSMYAHARCWIQVFEEHGV
                     HLDAIDILKREGMSGLESIADIFIDKGFSLPPDTILKNLQKKKLSLFSNYPIEVFPQI
                     YTIFPFLKSKGKNIGIVTGSFRYLVEQLMPQQLLQHVDVIISVDDVKRGKPYPDPYVK
                     ALEKLDVSNDSTIVIENAPMGIQAAKAAGLYCVAITTTLDSSFLKCADAILRDHNELI
                     VFLMNWCSS"
     gene            complement(3926..5092)
                     /locus_tag="PLJ75_11400"
     CDS             complement(3926..5092)
                     /locus_tag="PLJ75_11400"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015710135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPD05858.1"
                     /translation="MNNKEDIVQINKKKSVTKKLRLGIIYGGKSGEHDVSRCSAVSIA
                     EVASKKYEVVCIGITRDGRWYVQNAIHIDNHPSFGKVLAIHEHGKWAIDTSSSDGLLH
                     LLEKVTGEEVIVDVVFPIVHGTYGEDGTLQGLLEMVDVPYVGPGVLGSAVGMDKDIAK
                     RVLMNANGFAIVPFKTIRINEMNPQNKQKILRMVEKMLPVFIKPANAGSSVGISKVIN
                     KGMIEESLSLAFQYDTKVIVEKAIDCREFEVSVMGNEEPVASVVGEIIPQHEYYSYKA
                     KYLEKDGATLAIPATISKSLEHNLQKAACTIFSLLECEGLARVDFFVEKQTNILYFNE
                     INTLPGFTSISMYPKLWDCSGIPYTKLIDTLIHYALKRHRRRKKMVEAFLRLKK"
     gene            complement(5085..>5585)
                     /locus_tag="PLJ75_11405"
     CDS             complement(5085..>5585)
                     /locus_tag="PLJ75_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPD05859.1"
                     /translation="VMPREEMMEKFVISGVTSKDDEAKITIRDIPDRPGVASMLFGEL
                     GKQKVYVNMIVQSTGKDGKASISFTVLKNDLKKALTICEGLKATMGATSIDYKENIAI
                     VSAVGVGMLSSWGVAGRMFDVLFKHNINIEMISTSEIGISCVIDSMYTELAVKAIHKE
                     FIEVNE"
BASE COUNT         1676 a         1258 c          853 g         1798 t
ORIGIN      
        1 agcatgccag caactgatgc agcattttgt aatgctgaac gtacaacctt ggcaggatca
       61 ataatacctg cttttgtgag atcctcccac tccagtgttg ctgcattaaa gcctataccc
      121 tgtttttcct gttttgcctt ttcaacaaca accgatcctt caacaccagc gttaaatgca
      181 atcatcttca taggctcttc catagctttg gcaacaatcc gtacacctat tttttcatca
      241 ccatccagtt ttttctctaa ttctgcaaca gctttctgag catataacag cgttaaacca
      301 ccacccggga caattccctc ttctactgct gcacgagtag ctgaaagtgc atcttccacg
      361 cgagccttct tttctttaag ttcaacctca gttgctgcac caacatttat aaccgccaca
      421 ccaccagcta attttgccaa acgctcctgt aatttttctc ggtcataatc tgatgttgtt
      481 tcttctatct gcttcttaag aactttgatc cgtgcctgta catccgcctg gctgccagca
      541 ccttcaatta ttgttgtatt ttccttgtca ataataactt tttttgcacg tccaagcata
      601 tccatcgtgg tattttcaag ctttaatcca agatcttcag atatgacctg ccctttggta
      661 agaatagcaa tatcttccag cattgccttt ctgcggtcac caaatccagg ggcttttact
      721 gctacgcact gtataacctt gcgcaatgtg ttcacaacaa ttgtagcaag agcttcacct
      781 tcaacatctt cagcaatgat aagaaggggt ttgccagcct gtgcaacttt ttccagtata
      841 gggagaagat ctttcattgt agaaatcttc ttttcattga taaggataaa tgcatcctcc
      901 agtactgctt ccatattctc agggtcggtt accatatagg gagaaatata acccctatca
      961 aattgcatac cttcaacaac ttctagatgt gtttcaatag atttagcttc ttcaacggtg
     1021 ataacaccat ctttaccaac tttatccatt gcctcagcaa taagctcgcc tatttccatg
     1081 tcattattag cagaaatagc tgcaacctga gcgatctctt ttttatcctt aatttcacga
     1141 gctgtatcgc caataaattt tactgctact tctacagcct tatcaatgcc ccgttttaaa
     1201 ctcatgggat ttgcgccagc agtaacattt tttaatgatt catgatagat agcagctgca
     1261 agaatagttg ctgttgtagt gccatcaccc gccacatcat tggtctttgt agcaacttct
     1321 tttactaact gagcacccat attttcaaat ggatcttcca gctcaatttc ttttgccact
     1381 gttacaccat ctttggtgat agttggtgag ccaaattttt tatcgatgac aacatttcta
     1441 ccacggggac caagagttac ttttactgca tctgataatt ttattacacc ctgatagatt
     1501 gatcgccttg cttcatcatt gtattgtaaa atctttgcca tgtctttcac cttccttttc
     1561 tacgattttg taatttaatc caggatagct aaaatatcat tttcgcgaac aatcaaatat
     1621 tccttgccac catacttaac ctctgtacct gcatacttac caaacaatac agtatcaccc
     1681 gttttaacat ccaatggaac ccattttcca tcctcataac gacctttacc aacagccagc
     1741 accttcccct gctgtggctt ttcctttgct gtatcaggga tgtaaagact cccctgtttt
     1801 ggaagttctt cttccgtcac ttcaatgaga actcgatctc ctaatggttt gattcccaca
     1861 gtgttcctcc ttatccttga ttttaatgta ataattagat acttaaaaaa taaacaatca
     1921 ttctttttta caataatttt attattggaa taaaagataa aaaaattgtg ttagcactca
     1981 ataaaaagag tgctaacaac cattaatata gcaaaaaaat atttttctac caatattaaa
     2041 tataattatc taaaaataat taccagcatt tatagctttt tatttaaaaa tatgatatta
     2101 aatcatcgga aacaaaaatt tttgaggatt ttaataaaat ttttcaaaaa ttatctgtaa
     2161 aaatcacttg ccacatagtt actatgggat tggtttatgg tataaaccgc ttcattcaat
     2221 ggaaaagcgc aatcattact taaaatacct tagaaaagaa gggacatcgc ctacgattga
     2281 tgctgcaaaa taacaaatta tataattttg tatgattatt aacctcaccc ccaatctctc
     2341 cttcgcaagg cgagggaaac tatcacaacc gtcacttatc ttttaataat ccattgcact
     2401 tcaagaccta atatcggtaa tcctgtatca attgaataat aaaacttaat aatcctgata
     2461 agatgtaaag cgcaatgata accattatca cccattgttt aaacataaaa atgagcgcaa
     2521 ttaagattaa tataaacaat aataatttta tacccgtaat cttctgcaat ataaacgact
     2581 gaggctttgc atatcgcact gtcgatacca tgagcagagc aacaagaaca aaaccaatga
     2641 taaataaaag tttgggaata ataatattta taaaacataa tggcactaac gccacaataa
     2701 tacctgctat aggagatggc aatccatcaa aagcgttggg gacatgctgc acattaaaac
     2761 gcgccagcct gtatgctgca catacaggga aaattgctgc aatgaagata ccaatatcaa
     2821 taggtttatc aaggatataa atccatgttc cctgtagata gcctttaaat gctaaatatc
     2881 ctggagcgat gccaaatgta acacaatctg ccagtgaatc caattcctgt cctaacttgc
     2941 ttgcaacacc cagcatccgt gctacctgtc cgtctaatcc atccagtaaa gcagcaccta
     3001 aaatcaatac gcctgcaata gatagatgat gaggaatgtt catgcttgca aaaattattg
     3061 caataaagcc taaaactaaa ttgcccagtg tcaatgtgtt aggaatccat gcaacactca
     3121 tattacaccc acagttatta tttataaatt tttaacatct gtaaacacca ttaacgcaac
     3181 tataggtata tttacacacc tgtcaatgaa aaaacacact tcacgaggag caccagttca
     3241 ttaaaaaaac tatcagttca ttgtggtcac ggaggatagc atcagcacac tttagaaaag
     3301 acgaatctaa tgtagtggta atggcaacgc agtataatcc tgctgcttta gcagcctgta
     3361 tacccatcgg tgcattctca attactatag tactatcgtt gctaacatct aatttttcca
     3421 gtgctttcac atagggatca ggatatggtt tgcctctttt cacatcatca acgctgataa
     3481 taacatcaac gtgctgtaaa agctgctgcg gcattaattg ttccaccaaa taccggaatg
     3541 aaccggtaac aattcctatg tttttgcctt ttgatttaag aaaaggaaaa atagtatata
     3601 tttgcgggaa tacctcaatg gggtagtttg aaaaaagtga caattttttc ttttgaagat
     3661 ttttcaaaat agtatcgggt ggtaatgaaa aacctttatc aataaaaata tcagctatcg
     3721 attcaagtcc cgacatgcct tcacgtttta aaatatcaat tgcgtccaga tgaacgccat
     3781 gttcttcaaa tacctgtatc cagcatcggg cgtgcgcata catggaatca acaatcacac
     3841 catccatatc aaataacaca gtatcaacat aaagtccttt tatttttttt gtgcgatagt
     3901 tcatggtatt agtggttctg catgattatt tttttaacct gaggaatgct tccaccatct
     3961 ttttccttct gcggtgacgc ttcagtgcat aatggataag cgtatcaatg agtttggtat
     4021 aggggattcc tgagcaatcc cacagctttg gatacatact gatactggta aatccaggta
     4081 atgtatttat ctcgttaaag tatagaatat ttgtttgctt ttctacaaaa aaatcaaccc
     4141 ttgcgagccc ttcacattct aataatgaaa agatggtgca ggctgctttt tgtaaattat
     4201 gttccaaact tttacttata gttgccggta tagctaatgt tgcgccatcc ttttcaagat
     4261 attttgcttt gtatgaataa tattcatgct gtggtataat ctccccaaca accgatgcaa
     4321 caggttcttc attccccatc accgacactt caaattcacg gcagtcaata gccttttcaa
     4381 ctattacctt ggtatcatat tgaaaagcaa gcgatagtga ctcttcaatc atccctttgt
     4441 tgataacctt tgatatgcca actgatgaac cagcatttgc gggttttata aatacgggta
     4501 acatcttctc aaccatcctc agtatctttt gtttattttg aggattcatt tcattgatgc
     4561 ggattgtttt aaaaggaact atcgcaaatc catttgcatt cattaacact ctttttgcaa
     4621 tatccttatc catgccaaca gcagaaccca atacacccgg tccaacatat ggcacatcaa
     4681 ccatctctag aagtccctgc aaggtgccgt cctcaccata cgttccatgc actatgggga
     4741 aaacaacatc aacaatgacc tcttccccgg ttactttttc taaaagatgc agtaaaccat
     4801 cgctggagct tgtatcaatt gcccacttac cgtgttcatg gattgcaagc actttgccaa
     4861 atgaggggtg attgtcaatg tgtatggcat tttgtacata ccagcgccca tcacgggtaa
     4921 tgcctataca tacaacctca tattttttac tggcaacctc agcaatggat actgcggaac
     4981 atcgtgatac atcatgttcg ccacttttcc caccatagat gatgccaagc cgcagcttct
     5041 tagttactga tttttttttg ttgatttgaa ctatgtcttc tttattattc attgacttca
     5101 ataaattctt tatgaatagc tttaaccgct aattctgtat acattgaatc gataacacat
     5161 gatatgccaa tttctgaagt tgatatcatc tcaatgttaa tattatgctt aaacagcacg
     5221 tcaaacatgc gccccgctac accccatgac gacagcatcc caacaccaac tgcgctcact
     5281 attgcaatat tttccttata atcaatgctg gttgcaccca ttgttgcttt taacccttca
     5341 catattgtta atgccttttt aagatcattt tttaataccg taaacgagat agatgccttg
     5401 ccatctttcc ctgttgactg cactatcatg ttgacatata ccttctgttt acctaattcg
     5461 ccaaacagca ttgaagcaac gcctggtctg tcggggatat cccgtatggt aatctttgct
     5521 tcatcatctt ttgacgttac accgcttatg acaaattttt ccatcatctc ctcccgtggc
     5581 attac
//
LOCUS       DAOOJH010000125         5477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_11970_length_5477_cov_7.457765, whole genome shotgun sequence.
ACCESSION   DAOOJH010000125 DAOOJH010000000
VERSION     DAOOJH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11970_length_5477_cov_7.457765"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLJ75_11410"
     CDS             <1..593
                     /locus_tag="PLJ75_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05860.1"
                     /translation="FHDLITFSNTMSSFVEVINRIAEQVNLLSLNASIEAARAGEAGR
                     GFAVVADEISKLADATAQNASQIEKIISQTQNLIGSSNNSITDTSQFLSKLNGEIRTI
                     THEIAHTNTLIQDVNVSIKTIANLNNQIYTAIEKIETSTNEQRIANEEASKTIVYISD
                     SAQNLTDIITTLSQISRSIEEIAGSLNTLTQALMQS"
     gene            complement(602..1006)
                     /locus_tag="PLJ75_11415"
     CDS             complement(602..1006)
                     /locus_tag="PLJ75_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HPD05861.1"
                     /translation="MEFIEWTNALSVGVTTFDNEHKQLVVLINTLNQALTAGSAKKTM
                     EDILKSLVNYTTIHFKHEEDYMVLYDYPDYQKHKKEHDALTTQVIEFQERYKAGKTAF
                     SLELIHFLKDWLINHIMGTDKKYKDYFIAKGV"
     gene            complement(1016..1516)
                     /locus_tag="PLJ75_11420"
     CDS             complement(1016..1516)
                     /locus_tag="PLJ75_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPD05862.1"
                     /translation="MKKKYIILFLAIVIVNVFVFSIFSQVKWYDFNAGYSAVKNSKKP
                     AVIDFYADWCIWCKTMDKETFGNPMVAKKMGNDYIAIRINVENNEAIVFDNKQYTPQE
                     FASYCGVDGLPTLVFMDKKGQLVTRIPGYIKSDVFLSLLDYMKGECYKKKVSFSDYMK
                     DKGCNK"
     gene            1888..2700
                     /locus_tag="PLJ75_11425"
     CDS             1888..2700
                     /locus_tag="PLJ75_11425"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPD05863.1"
                     /translation="MYVKRILSILLILTIFLIESCDNDIFKYSIHEIDSKYSGSDLND
                     VNAALIDSLYNNPLEDFTFAVVTDSHTDYDKLDSAVSIINANPSIKFVIHLGDMTDTG
                     LLLQYEMTVRILKKLHVPFVAAIGNHDLLGNGFIIFKDIFGHTNFSFTCNDVFFIIAN
                     DNNWESSIGDILQQFNDLLVHHQDARYRIIATHIPFTDNARYFQEFLDDYQYLCEDYD
                     VALSLHGHRHRHYYIPDFTSKTAMLSANAIVHNKLYLIHVMATGIEYEIVGF"
     gene            2710..3294
                     /locus_tag="PLJ75_11430"
     CDS             2710..3294
                     /locus_tag="PLJ75_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05864.1"
                     /translation="MKKVCITIIFLFLYLNPLYSQLKYKQERSFIPDFVVSQFAGYIG
                     FISSGVGYDFYDESLKLELLYGYVPKSVGGIRIHMFTGRGSYSLLRYRTTNELSFYPL
                     NLSFFINYARGRQYVLRWSPTYPERYYRPTSLYTGESVGMSIKKSCENATIKGYEFYA
                     DVVTMSEYLYEYCKNETVKLEYIISLAFGVRIYF"
     gene            3410..3814
                     /locus_tag="PLJ75_11435"
     CDS             3410..3814
                     /locus_tag="PLJ75_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HPD05865.1"
                     /translation="MLASPCGIFCAACPRFYKFGICKGCRIDNLHDNCEIYDCCIRIG
                     GMDFCFECECFPCERLKQFSNYHHGENFAHYRHIIIDNLQQIKAMGVEKWYSIMNNRF
                     MNGEYGIQQRNPDGSFDISPCLCCKSTNHKNS"
     assembly_gap    4017..4116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4117..4350
                     /locus_tag="PLJ75_11440"
     CDS             <4117..4350
                     /locus_tag="PLJ75_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05866.1"
                     /translation="QDITDYFIQAQEDDYKEGAGLGLVLITMMFKGMGLSQKHFFIDS
                     DFEKTIASMHVPLTATTIQEYEKRLEETTPKSA"
     gene            4355..5143
                     /locus_tag="PLJ75_11445"
     CDS             4355..5143
                     /locus_tag="PLJ75_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HPD05867.1"
                     /translation="MKTLAFSSCPNDTFIFHALVHSLIDINGLSFSTHIDDVESLNHA
                     ALNQQYDITKLSFHGFLHVQDNYELLDAGAALGFGCGPLVITRKGLNNIQHAVIATPG
                     IYTTAHLLFRLCFGNSNELVHVRFDEILPGIQQGIFDAGVIIHEGRFVYQNYNCDMLI
                     DLGKWWEDVTGLPIPLGCIAIHKKHSAQKSLIEEMIRNSLDYARRHPYDSKEYIHKLA
                     QELDETVIQQHIDLYVNDFSVSLGHTGHNALAALREMAQCQNLL"
     gene            5125..>5477
                     /locus_tag="PLJ75_11450"
     CDS             5125..>5477
                     /locus_tag="PLJ75_11450"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05868.1"
                     /translation="MSKPAIVIQCATHTEAKPFINYFHCTVLQQKPFKIFLGDSSLHI
                     LIGGIGITKAAIAATYACLAYDVQHIINIGAAGALALQLLPGDICCVKSVIDNSRYNL
                     TTLEPFTYTLAPIDE"
BASE COUNT         1784 a          983 c          897 g         1713 t
ORIGIN      
        1 agtttcatga tctcattacg tttagcaata ccatgtcatc atttgtagaa gtaattaacc
       61 ggatagcaga gcaggttaat cttttgtcgc tcaatgcatc gattgaagct gcacgtgcag
      121 gtgaggcagg gaggggattt gcagtggtag cagatgaaat atccaaatta gccgatgcaa
      181 cagcacagaa tgcatcgcag atagaaaaaa ttatttcaca gactcaaaat ttaataggat
      241 caagcaataa cagtattact gacacatcac aatttttatc aaaactaaac ggagaaatac
      301 gtaccataac gcacgaaata gcacatacca atactcttat tcaggatgta aatgtttcaa
      361 taaaaactat cgcaaatctc aataatcaga tatacaccgc aattgaaaaa attgaaacat
      421 caaccaatga acagcggatt gccaacgaag aagcatcaaa gacaattgta tatatttcgg
      481 actcagcaca aaacctaact gatataatta ctactctatc acagatatcc agatcaatcg
      541 aggagattgc aggaagcctt aatacattaa cgcaagcgct gatgcagtcg taaaaggaat
      601 gttatacacc ctttgcaatg aaataatctt tatacttttt gtcagtaccc atgatgtgat
      661 tgataagcca gtcttttaaa aaatgtatca actccaatga aaatgcagtt ttgccagctt
      721 tatagcgttc ctggaattct atgacctgcg tggtgagcgc atcatgttct tttttatgtt
      781 tctgataatc gggataatca taaagaacca tataatcttc ttcatgctta aaatgtattg
      841 ttgtatagtt aaccagactt ttaagaatat cttccattgt tttttttgcg cttcctgctg
      901 ttaaagcttg atttaatgta ttgattaata ctaccagttg tttatgctca ttatcaaatg
      961 ttgttacgcc aacactcagc gcattagtcc actcaataaa ttccataacc accccttatt
     1021 tattacaacc tttatctttc atataatcac tgaacgatac ctttttttta tagcattcac
     1081 ctttcatata atcaagtaac gataaaaaaa catctgattt aatatatccc ggaatacggg
     1141 taacaagctg tcctttttta tccatgaaca caagtgttgg taatccatca accccacaat
     1201 atgaagcaaa ttcctgtggg gtatattgtt tattatcaaa tacaatagct tcattatttt
     1261 caacattgat acgtatagca atatagtcat tccccatttt tttggcaacc attggattcc
     1321 caaaggtttc tttgtccatc gttttacacc atatgcacca atctgcataa aaatcaataa
     1381 ctgctggctt cttgctattt tttactgcgg agtatcctgc attaaaatca taccacttaa
     1441 cctggctaaa aatactaaaa acaaacacat ttacaataac tatcgccaga aatagtatta
     1501 tatatttctt cttcatggtc agcacactcc attatatttt tcattgtgat tttgcactct
     1561 atagtacgct aacatttatt atggtacttg taaacctgtt ttttacttta cattaaaaat
     1621 actgcaagaa aataagtaat caaaaaaaat catcatagca tattaatgct aatgagtaac
     1681 aaatcactga cactaatgaa attgcataac ctgcatacat tgtggcatat ttcatactat
     1741 gcgttactat ccttaaccac caataccatt attgcactat ggatggtcat tgaaacttta
     1801 tgacttccat aatcaatttt aattaaacag ttaattaata tattgacaga tagtgcatag
     1861 cacaaattat tgtcacagga ggaaaaaatg tatgtaaaac gtatactatc aattttatta
     1921 atattgacaa tttttctgat agagagttgt gacaatgata tctttaagta cagcatccat
     1981 gaaattgata gcaaatacag cggcagtgat cttaatgatg tcaatgctgc attaattgac
     2041 tcactgtaca ataaccccct tgaagatttt acctttgccg ttgttaccga ctcacatacc
     2101 gactacgata agcttgacag tgcagtaagc attatcaatg caaatccaag cataaaattt
     2161 gtcatccatc ttggtgatat gacagataca ggattattac tacaatacga gatgactgtt
     2221 cgtattttaa aaaagctgca cgtgccattt gtagcggcaa taggcaatca tgatcttctt
     2281 ggcaatggat tcattatttt taaggatatc tttggacata caaatttttc atttacctgc
     2341 aatgatgttt tttttatcat tgcaaatgat aacaactggg aatcgagcat aggcgacata
     2401 ttacaacaat ttaacgatct ccttgttcat catcaagatg cacgatatag aattatcgct
     2461 actcatattc cctttactga taatgcacgt tattttcagg aatttttaga tgactatcaa
     2521 tatctttgtg aagattacga tgttgcgctt tccctccatg gacacaggca tagacactac
     2581 tatataccgg attttaccag caaaactgcc atgctcagtg caaatgcgat agtccataat
     2641 aaattatatc ttatccatgt aatggcaact ggtatagaat atgaaattgt tggattttaa
     2701 caaaacatca tgaaaaaggt atgtataacc attatttttt tatttcttta tttaaatccc
     2761 ttgtattcac agctaaagta taaacaagaa agatctttta ttcctgattt tgttgtttca
     2821 caatttgctg gatatatagg gtttatttct tctggagtgg gatatgattt ctatgatgaa
     2881 agtcttaagc tggaattatt atatgggtat gtccctaaat ctgttggtgg aatcaggatt
     2941 cacatgttta ccggtagagg ttcttattca ttgctacgct atagaactac gaatgaatta
     3001 tcattttatc ctctcaacct ttcatttttt attaactatg cccgcggtag gcaatatgtt
     3061 ttacgatggt caccaacgta cccagaaagg tactacagac caacctccct ctatacggga
     3121 gaatctgttg gcatgagtat aaaaaagagc tgtgaaaacg ctaccatcaa aggttatgaa
     3181 ttttatgccg atgttgttac gatgagcgaa tacctttatg aatattgcaa aaatgaaacg
     3241 gtaaaattag agtatatcat aagccttgct tttggtgtta gaatttattt ttaataaagc
     3301 ttgctatagt gtttacctat aaaaataggc aatttttttg gaaacaataa aaaaaatgat
     3361 acgcttttat taataaaatg tacttgcaac attagctatg aatgaaaaaa tgttagcgtc
     3421 cccctgcggc attttctgcg ctgcctgtcc caggttttat aaatttggaa tctgcaaggg
     3481 ctgtagaatc gataaccttc atgataactg tgaaatatat gactgctgta tccgcatagg
     3541 gggcatggat ttttgctttg agtgtgagtg tttcccctgt gaacggctta aacagtttag
     3601 caattatcat catggtgaaa attttgccca ttaccgccat attattattg ataatctaca
     3661 acagattaaa gcaatgggcg ttgaaaagtg gtatagtatc atgaataata gatttatgaa
     3721 tggagaatat ggcatacaac aaagaaatcc ggacggttct tttgacattt ccccctgttt
     3781 atgttgtaaa tcaacaaatc ataaaaattc ttgacaaaaa ccatttatga tagtagcttc
     3841 aaaaaaggtg atatgaggga atatatgact atcgatgaag ccatagagca aaataaaaaa
     3901 attatcatta aagcatattc tgtaagcgaa tcaatagaat attttttaaa agttgcttta
     3961 acaaaagttt tacatgccta tagctgtgat atgctgttga taccgctcta taccagnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncagg acatcactga ttattttatt
     4141 caagcacagg aagatgacta taaagaaggt gcggggttgg gactggttct tatcaccatg
     4201 atgtttaaag gcatgggctt atcacaaaaa cattttttta ttgactcaga ttttgaaaaa
     4261 acaatagcat ccatgcatgt accgttaact gcaaccacaa tacaggaata tgaaaagcgc
     4321 cttgaagaaa caacacctaa gagtgcctga taccatgaaa actttggcct tttcatcatg
     4381 ccccaacgac acctttattt ttcatgccct tgtacactca cttatcgata tcaatggatt
     4441 atcgttttca acccatattg acgatgttga atcattaaat catgctgctc tgaaccagca
     4501 atacgatatt acaaaactat cgtttcatgg atttttacat gtacaggata attatgaact
     4561 tctggatgct ggtgccgcat tagggtttgg ctgcggacct cttgtaataa cacggaaagg
     4621 attaaacaat attcagcatg ctgtcatagc aacaccgggg atatacacaa cagcacatct
     4681 tttattcagg ctttgttttg gtaacagtaa cgaacttgtc catgtcagat ttgatgaaat
     4741 tttacccggc attcagcaag gtatttttga tgcaggggtt ataatccacg aagggcgctt
     4801 tgtatatcaa aactataatt gtgatatgct tattgactta ggaaaatggt gggaagatgt
     4861 aaccgggctg cccatcccat tgggatgtat tgcaatacac aaaaaacata gtgcacaaaa
     4921 aagccttata gaagaaatga tacgtaattc actagattat gcacgcagac atccatacga
     4981 ttcaaaagaa tacatccaca agcttgcgca ggaacttgat gaaacagtaa tacagcagca
     5041 cattgatctt tatgttaatg attttagcgt atcattagga catacgggtc ataacgctct
     5101 ggctgcactc agggaaatgg cacaatgtca aaacctgcta tagtgataca atgtgcaaca
     5161 cacacagaag caaaaccatt tattaattat tttcattgca ctgtattgca acaaaaacca
     5221 tttaaaatat ttttgggcga tagctccctg catatactca ttggcggaat tggcattaca
     5281 aaagcggcaa tagctgcaac atatgcctgc cttgcatatg atgttcagca tattattaac
     5341 ataggtgcag caggagcact tgccttgcaa cttttgccgg gagatatatg ttgtgtcaaa
     5401 tctgttatcg ataattcacg atataatctc accacgttag aaccttttac gtataccctt
     5461 gcacccattg atgagtt
//
LOCUS       DAOOJH010000126         5466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_12000_length_5466_cov_9.899649, whole genome shotgun sequence.
ACCESSION   DAOOJH010000126 DAOOJH010000000
VERSION     DAOOJH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12000_length_5466_cov_9.899649"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1883
                     /locus_tag="PLJ75_11455"
     CDS             <1..1883
                     /locus_tag="PLJ75_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05869.1"
                     /translation="ISLSTVSYLIDTYSITNLRVAGTVGYDWNLCIGVRSDNPVLYSV
                     IAKALNSLPKTKRDEIYRKWISLAPRPVYYEIQFWYIIASILLGVSIIILVIIGWNTS
                     LRKQVAIKTQQLENELQLHKKTQEQLLLEKERLFITIQSIGDIFIITDNEGNVILANK
                     QAETVFGKAVVSGGCLYSSITFIDPNAQHTVNPIDACMQSGNVTTFYHCICRYDDRSF
                     DVSVVSSPIIIEDTVTGVVTIMRDISTMLAMQNELIKLQQFESLGILGAGIAHDFNNI
                     LMAIMGNTELLGAVLKNTGNVNDSTRITEGIKKSVKSAQSLTAQLLTYARGGKPIKQS
                     GNIVQLLNDTVDFMVHGTGVTINYSIDSYDYEFDFDESQLSHVFRNLTLNAVQAMGNR
                     GVLTIKVLEESMDTLNTIQLPAGNYVKIQFIDTGNGIPPEHVNKIFYPYFTTKEKGSG
                     LGLTTSLSIVKQHGGTMTVSSSHEGTTFSVYLPKVKQVQVNDTAMAHEVASPNAVVLV
                     IDDRQDIIEVTTGMLDILGYSYDWAYSVNEAIAKLVEGKNKKHLFDCVLVDVTIPGDL
                     SGVEAFTKMKTIQPLLKGIVMSGYADNNAIAEYDRYGFSWYLVKPFTLSDLKLALLKA
                     LL"
     gene            2124..3350
                     /locus_tag="PLJ75_11460"
     CDS             2124..3350
                     /locus_tag="PLJ75_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPD05870.1"
                     /translation="MDKEKIMDLYAQHVSPGKVEMYKKYDIALVPHKRRGVYLYDISG
                     KRFINCHCNGGVFNLGHRNKIIMDAVKKAMQRYDIGNHHLISKPKALLANKLAATLPQ
                     GLNQVIYGVSGGEAIDTAIKLARAVTGRPTVLSAIGGYHGHTGFALATGDEKFKKPFE
                     PMPPGFLQVPFNAIDALESAIDDKTAAVIFETIPATLGIVVPDLDYFKKVQALCERHG
                     ALFIIDEVQTGFGRTGAMWGFQHFDVTPDIVVMGKGMSGGIYPITATVYKEQYAYFIK
                     ENPFIHISTFGGSEIGCFAAMAVLDLCDSNLLAHVQTMENYFKEKLAALTLHYPETPF
                     TVHGLGLMLGLAFHDEMTALFMVKLLYDNGVYVVYSGNDPRVIQFLPPLIITKKEADM
                     VMAAIDKSLEALSQSK"
     gene            3360..4889
                     /locus_tag="PLJ75_11465"
     CDS             3360..4889
                     /locus_tag="PLJ75_11465"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HPD05871.1"
                     /translation="MVDDYKDISEHLVFLNKVYELKRKRIRFMQLATVDRQLHTMADN
                     FYSLWNKTSKGFILKDAHIHESSIDTLKGLFGLHDEITIISKGDKAILTFDKLRFYQY
                     LVRHVANINACSYLYNIFYGEENISINTLVKEQLPYLERYIPTFKKFDKAKDITSFAF
                     SQFDIENLWSIIEIYDRIKDNLIKDTALYKQFVNDFTRLYREDIALKILGYGEISTVM
                     QTIHGWQIDKTLLKMQLYEPDWVWKRMPPINSFQDVEALKKAYHEYREILIQKIGIQV
                     PEQQFRYFKNNGWYTVYAGQKKINPQMVGNVLVKRLDEVNALALFNKVLSELKRWYLF
                     NKTDSSIQVGIDGQISNWVLLSADGALNYVGSNDTLLYIDTSTPLYRINGVEQLNAEL
                     FLKNIPWFLRSIIRALFLQEVLDRYYDLRSVVVDCIANLYKEKREDLIPACIKAANYF
                     FSTMDESLQPLTLHEIVKYYQGDAFIWRLFQFARKVDKFVTERIFQKKYMYRLPKKIE
                     R"
     gene            complement(4893..5390)
                     /locus_tag="PLJ75_11470"
     CDS             complement(4893..5390)
                     /locus_tag="PLJ75_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05872.1"
                     /translation="MKQQNIIPLNKVEKTERYIVVPRLIKGKVHKVVKPLKKIKGNCL
                     FTCNMPHTLIDFIYRSVKKLHLQDNKYIFSRGNVYINNKVVHNAVSTVTLEWDLGTSF
                     IIPLRLQFNSFVIIEVDNKDYSVRLMELAILIALMAQAYPSLPRSKHVSNAQRVLTLG
                     WKSIE"
BASE COUNT         1836 a          839 c         1068 g         1723 t
ORIGIN      
        1 taatctctct ttcaactgtt tcatatttaa ttgacaccta cagtataaca aatttacgtg
       61 ttgcgggaac cgttggctat gattggaacc tgtgcattgg tgttcgcagt gataatccag
      121 tattatattc agtcattgct aaagcgctca attctttacc aaaaacaaaa cgagatgaga
      181 tttacagaaa gtggatttca ctagcaccaa gacctgttta ttatgaaatt caattctggt
      241 atatcatagc atccatactt ttaggagtgt caattattat actggttata attgggtgga
      301 atacttcgct aagaaaacag gttgcgataa aaacacagca acttgaaaat gaattacaat
      361 tgcataaaaa aactcaggaa cagttgttgc tggaaaaaga acgtttgttt attaccatac
      421 aatctattgg tgatatattt atcatcactg ataatgaagg gaatgtgata ctggcaaata
      481 aacaggcaga aacagtattt ggaaaagcag ttgtatcagg agggtgctta tattcttcca
      541 ttactttcat tgatccaaat gctcaacaca ccgttaaccc tatagatgca tgtatgcaat
      601 cgggtaacgt aactacattt taccattgta tatgtagata tgatgataga tcatttgatg
      661 tttctgtagt ttcttcacct atcatcattg aagatactgt cacgggagtt gtaactataa
      721 tgcgtgatat aagcaccatg cttgcaatgc agaatgaact tataaaactg caacaatttg
      781 aatcattggg aatactgggt gctggcatag ctcatgattt taataatata ctcatggcaa
      841 ttatgggtaa cacagagttg cttggagctg tgttaaaaaa tacaggcaat gtaaatgata
      901 gcacccgtat aactgaaggg attaaaaagt cggtgaaatc ggcacagtct cttacggcac
      961 agttattgac ctatgccaga ggtggaaaac caataaaaca gtccggtaat attgttcaat
     1021 tgcttaacga cacagtagac tttatggtac acggaacagg cgtaacaatt aattatagta
     1081 tagatagtta tgattatgaa tttgattttg atgaaagtca actatcgcat gtattcagaa
     1141 atttaacatt aaatgcggtg caagctatgg gcaacagggg ggtgttgacc atcaaggtat
     1201 tagaggaaag tatggatact ttaaatacca tacaactgcc tgctggtaat tatgtaaaaa
     1261 tacagtttat tgacacagga aatggcatcc ctccggaaca tgtaaataaa attttttatc
     1321 cgtattttac aacaaaagaa aagggttcag ggcttggttt aactacatcg ctttccatag
     1381 ttaaacagca tggtggaact atgactgtat catcttcgca tgaagggaca acatttagcg
     1441 tttatttacc aaaagtaaaa caagtacagg ttaatgatac cgcaatggct catgaagttg
     1501 catcacccaa tgccgtggta ttggtaatag atgaccgtca ggatataata gaagtaacaa
     1561 cagggatgct ggatatatta ggatatagct atgattgggc ttatagcgtt aatgaagcaa
     1621 tagctaaact ggttgaaggg aaaaacaaaa aacatctgtt tgattgtgta ctggtagatg
     1681 taacgatacc aggggatttg agtggagttg aagcatttac caaaatgaaa acaatacagc
     1741 cacttttaaa aggtatcgtc atgagcggtt atgctgataa taatgcaata gctgaatatg
     1801 accggtatgg attttcatgg tatctggtaa aaccattcac attaagtgat ttaaaacttg
     1861 ccctgctaaa agcgcttttg taaccaggtt acaatggcag cagtcactac tattgaaact
     1921 attaaactat ggttatttgt atctcagcca ctggtaactc ttttataata gactacatat
     1981 tcacaatata aataatgagt atgaaataat gattatcatt tatatattat atgatgattc
     2041 aatttacagt gactatcgca catgaaaaaa aaaagatttt gacatattta taaaagtatt
     2101 gtaatataaa acatgggggt tttatggata aagaaaaaat tatggatttg tatgcacagc
     2161 atgtctcgcc cggcaaagtg gagatgtata aaaaatatga tattgctctg gtgccccata
     2221 agaggcgagg cgtttattta tatgatatct ccgggaaacg gtttattaac tgccattgca
     2281 atggtggtgt ttttaattta ggacacagaa ataaaataat aatggatgcc gtaaaaaaag
     2341 cgatgcagcg ttacgacatt ggcaaccatc atttaataag caagccaaaa gctttactgg
     2401 ctaacaaatt agctgctacg ttaccacagg gattgaatca ggttatttat ggtgtttcag
     2461 gtggcgaggc aattgatact gcaataaaat tagcacgagc tgtgacaggc cgccctactg
     2521 tattatctgc aattggtggt tatcatggac acaccggatt tgctctcgca acgggtgatg
     2581 aaaaatttaa aaagccattt gaaccaatgc ctccgggatt tctacaggtt ccattcaatg
     2641 ctattgatgc attggaatca gcgattgatg acaaaacagc agccgttata tttgaaacaa
     2701 ttccagcaac gctgggtatt gttgttcccg atctggatta ttttaaaaag gtacaggcgc
     2761 tgtgtgaaag gcacggtgca ctatttatca ttgatgaggt gcaaacaggc tttgggcgta
     2821 caggcgctat gtgggggttc cagcattttg atgttacgcc agatattgtt gttatgggta
     2881 aaggaatgag tggtggtatt tatccaataa ctgctacagt atataaagag cagtatgcat
     2941 atttcattaa agaaaatccg tttattcata tctcaacgtt tggcggcagt gaaataggtt
     3001 gttttgctgc aatggcagtt ttagatttgt gcgatagtaa ccttttagcg catgtgcaga
     3061 ccatggaaaa ctattttaaa gaaaagcttg cagccttaac tttacactat cctgaaactc
     3121 cttttacagt acatggattg ggattgatgt taggacttgc atttcatgat gagatgaccg
     3181 cactgtttat ggtaaagctt ttatatgata atggtgtata tgttgtgtat tcaggaaatg
     3241 atccgcgggt gatacaattt ttaccacctt tgatcattac aaaaaaagaa gctgatatgg
     3301 taatggctgc cattgataaa tcacttgaag ctttatcgca atctaagtga ggtgtcgtta
     3361 tggttgatga ctataaggat atttcagaac atttagtctt tttaaataaa gtttacgaac
     3421 taaagcgaaa acgcatacgg tttatgcaac tggcaacagt ggacaggcag ttgcatacca
     3481 tggcagataa cttttattca ttgtggaata aaacatcaaa aggatttatt ctgaaagatg
     3541 cacatattca tgaaagtagc attgatacat taaaaggatt gtttggattg catgatgaaa
     3601 taactattat ctctaaaggt gataaggcta tacttacctt tgacaaatta cgattttatc
     3661 aatatttagt gcgacatgtg gcaaatataa atgcctgtag ctatttatat aacatttttt
     3721 atggtgaaga aaacatttct ataaacacat tggttaaaga acaattacca tatcttgagc
     3781 gatatatccc tacatttaaa aaatttgata aagcaaagga tattacatca tttgcttttt
     3841 cacaatttga tattgaaaac ttatggtcaa ttattgaaat atacgacaga atcaaagata
     3901 atttaataaa agatactgct ttatataaac aatttgttaa cgatttcaca cggctttatc
     3961 gcgaggatat tgcacttaaa atattgggtt atggtgaaat ctccacagta atgcagacca
     4021 tacacggttg gcaaattgat aaaacattgc taaaaatgca attatatgaa ccggattggg
     4081 tgtggaagcg tatgcctcct ataaattctt ttcaggatgt tgaggcgctg aaaaaagcat
     4141 atcatgaata tagagagatt ttaatacaaa aaattggtat acaggttcca gagcagcaat
     4201 ttcgttattt caaaaacaat ggatggtata cagtatatgc agggcagaaa aaaattaatc
     4261 cgcaaatggt tggcaatgta cttgttaaac ggttagatga agtcaatgcg ctggcacttt
     4321 tcaacaaagt attatctgaa ttgaaaagat ggtatttatt taataaaact gatagttcta
     4381 ttcaggttgg tattgatgga cagatatcca actgggtgtt actatctgct gatggtgcac
     4441 ttaattatgt gggcagcaac gatacattgc tttatatcga taccagtacg ccattatatc
     4501 gcatcaatgg tgttgagcaa ctcaatgcag aacttttttt aaaaaatatt ccgtggtttt
     4561 tacgatcaat tatacgtgct ttatttttac aggaagtact tgatcgttac tatgatctac
     4621 gatcagtagt ggttgactgc attgcaaatt tatataagga aaaacgagag gatttaatac
     4681 cagcatgtat aaaagcagca aattatttct tcagtacaat ggatgaatca ctacagcctc
     4741 ttacacttca tgaaattgta aaatactatc aaggcgatgc ttttatctgg cgtctttttc
     4801 agtttgcacg taaggtagac aagtttgtaa ctgaaaggat tttccagaag aaatatatgt
     4861 atagattacc aaaaaaaata gaacggtaaa agttattcaa tacttttcca tcccagtgtc
     4921 agtacccgtt gtgcattgct tacatgcttg cttcttggta gtgaagggta agcttgtgcc
     4981 atcagcgcaa taagtattgc taattccata agtcgtacac tataatcttt attatcaacc
     5041 tcaattatca caaaggagtt aaattgtaat cgtagcggaa tgataaatga tgttccaaga
     5101 tcccattcta aagtaactgt cgatacagca ttatgaacaa ccttattatt gatgtataca
     5161 ttccctcgag aaaaaatata tttgttatcc tgaagatgta atttctttac acttctgtaa
     5221 ataaagtcaa taagagtatg gggcatatta caggtaaaaa gacaattgcc ttttattttt
     5281 tttaatggtt taacaacttt atgcactttt cctttaatca ggcgtgggac aacaatataa
     5341 cgttcggttt tttcaacctt gtttaatggg ataatatttt gttgtttcat aaatggtaaa
     5401 agtaattgta taatgtactt gattaaattg gcacatggtt ctattattag tcaatcataa
     5461 aaaaat
//
LOCUS       DAOOJH010000127         5330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_12367_length_5330_cov_8.445118, whole genome shotgun sequence.
ACCESSION   DAOOJH010000127 DAOOJH010000000
VERSION     DAOOJH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12367_length_5330_cov_8.445118"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLJ75_11475"
     CDS             complement(<1..248)
                     /locus_tag="PLJ75_11475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD05873.1"
                     /translation="MKVIACLGNPGKKYSNNRHNAGILVGAAIAQHYSITVGKKEHDA
                     LTGSGIIEGHECIFMIPQTYMNNSGIAVQKVMSYYKELP"
     gene            complement(256..597)
                     /locus_tag="PLJ75_11480"
     CDS             complement(256..597)
                     /locus_tag="PLJ75_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD05874.1"
                     /translation="MKITSTKINGVVLIKVEGSLATEQIKQFEKELYTALDKNSNVII
                     DFNELSFICSAGLSLLIASHKKAEEKKLRLVLTGCSDDILKLFSLTEIDQHLNIVNSI
                     EEAQSLINAMK"
     gene            864..1817
                     /locus_tag="PLJ75_11485"
     CDS             864..1817
                     /locus_tag="PLJ75_11485"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPD05875.1"
                     /translation="MRAARIVAPGKFTVDNDPIPDCSDSHVRVKLLKAAICGSDIPYF
                     TSVYSRESYPLPPGYPGHECLGVIEASRVKQFKEGTPVMYYPPFLDGFKEYHVTPPSR
                     VQSIPDTLPLNELLITQLLGAVSHCAFRIDKPYNKHVAIMGQGPVGLLFTALMKNFGA
                     RSVIAIDPLDYRCKAAINMGATHTINPHTSDLLEAIKAITDGSMADIVIDAYGQDVAV
                     INKCFEIARHNGQVAFFGICLEEAPRLNFNTFFRKELRMIASVGPDLTMDYPYALDMI
                     LKGAIDVTPLLTHDIPFEDIQKGFEMASNREDGAIKIVLSF"
     gene            1849..2310
                     /locus_tag="PLJ75_11490"
     CDS             1849..2310
                     /locus_tag="PLJ75_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05876.1"
                     /translation="MPRAKIYGYIAVIVAALLGCAFVYYAFIHYDMYASVHFDSIDTG
                     AYWAVVLPIALVTLFVLATGFWVGYTILTIKVAPPMPEIIEKKDNSKFKAFLLCLVTI
                     ALAVWFVYGLYHHSYLAIAIPAACITLVILGMVFWVGIAIITTRSTLKNNK"
     gene            2317..3555
                     /locus_tag="PLJ75_11495"
     CDS             2317..3555
                     /locus_tag="PLJ75_11495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD05877.1"
                     /translation="MRALFLCYRGNPFCGGQGIYLYNLTKELAKLGVAIDVIVGPPYP
                     DPLDEWATVYKLENLNMWAIKTKHFPYEKLIKLYTPWHFVDYCLTRFHIFPEMETFSM
                     KAFFLLRKLLKTNHYDIIHDVQCLGWGLLPMKGYGIPIVSTVHHPLTKDREADFLVDN
                     TVWEMACTILFYPLTMQRIVINRLDRVITSSKEGINALNEAFHLPPEKVSVVDNGMDV
                     EFFRNNGNPRKENTLLFVGNTEDHKKGLRYLFDAMTMLPEHVTLTIVDDGPPKRLTAW
                     GWITERGLEKRVTFTGKVDLPTLVHLYSTSTILVMASLYEGFGLPAAEAMACNTPVVV
                     TSAGALPEVVGDCGVIVPPRDAVALKEGIVRLLNNKALREELGKKGRKRVEDNFAWPV
                     AAKNTLAVYHDVIQSYRSRI"
     gene            3552..4784
                     /locus_tag="PLJ75_11500"
     CDS             3552..4784
                     /locus_tag="PLJ75_11500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD05878.1"
                     /translation="MKICLMSYRGNPYCGGQGIYLMYLARELAKLGHEVHAIVGPPYP
                     FPVEGVTYHYVSNHNYFNIKDFVKPHKPFATFNPLNFYEFVASKLGIFPEIESFSFRA
                     YSKLRQLLKTHSFDVIHDNQCLGYGFLLIKQFGIPFISTIHHPLTIDRSTWFEYPSSF
                     FLKTKRILYYPLVMQKYVANNMDLIVTVSHDSAQEIYNAFGVPLEKQRVVYNGMDSSI
                     FNPVKLPKRPNSLIFVGNVEDRKKGVIYMLKALTLTRHKVHLTIVDGGAPNRNYVPRW
                     IDKFNLNEQITFTGKIPLDKLVQLYAQTQIALCPSLYEGFGFPAAEAMACELPVIAST
                     GGALPEVVGQHMKTGILVPPRDPYALAEAIDFLVENPDIRNRIGKAARQRVLKTFTWE
                     NAARQMVNVYQEAIDAYR"
     gene            4771..>5330
                     /locus_tag="PLJ75_11505"
     CDS             4771..>5330
                     /locus_tag="PLJ75_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD05879.1"
                     /translation="MLTVDFDLLAVKDNNIALDAGCGLGRHSFEFVSRGANVFSMDMD
                     MDCVRRTRFTLVYMRDNGQAKQGTHFQVLLGNALKLPFRDNTFDRVICAEVMEHVPDD
                     NQACHELARVLKPGGLIAITVPTYISEMLYDTLTYEYFTSPGGHIRKYVPKKLAAIME
                     NNGLKIYAVDFRHSFHTIYWLIRCVVG"
BASE COUNT         1544 a         1066 c         1089 g         1631 t
ORIGIN      
        1 ggcaattctt tgtaatatga catcaccttt tgcactgcta tgccgctgtt attcatgtac
       61 gtttgcggta tcatgaaaat acattcatga ccttcgatta taccgcttcc ggttagtgca
      121 tcatgctctt tctttcctac tgttatactg tagtgctgcg ctatagcagc acccacaagt
      181 ataccggcat tatgacgatt gttagaatat ttttttccgg gatttcccag gcaggctatt
      241 actttcattt gctaattatt tcattgcatt aatcagtgac tgcgcttctt ctattgagtt
      301 tacaatattg agatgttgat caatttcagt taacgagaat aattttaata tatcatcact
      361 gcacccggtt aaaacaagcc ttaatttttt ttcctctgcc tttttgtgtg atgcaatgag
      421 caaacttaaa ccagcactac atataaacga tagttcatta aaatcgatta ttacattgct
      481 atttttatca agtgccgtat atagttcttt ttcaaactgc ttgatttgtt ctgtagcaag
      541 cgaaccctca acttttatca ataccacccc atttatcttt gttgatgtga tcttcattcc
      601 caaacccctg aagctactat ataataaact ataggcatat atcctaaaac ataaataata
      661 attgtcaagt aaaatcatta tatcataggt acaactattt aaaaaatttt taagttaaac
      721 aacaatgaac cactatatac atgttaacta ttcaaatacg ctttaaaatg tcaataaata
      781 gattgacaat aaccatatga aataacattg tgtagttatg atttatttgt atatattaat
      841 ttattttatc aggatttcac tctatgcgtg cagccaggat tgttgctccc ggaaaattta
      901 cagttgataa tgatcctatc cccgattgtt ctgacagtca tgtcagggtt aagttattaa
      961 aggcagcgat atgtggttcg gacataccct attttacatc agtttattcc agagaatcct
     1021 acccgctgcc acctggatat cccggccatg aatgtctggg tgttatagaa gcatccaggg
     1081 ttaagcagtt taaagaagga actcctgtaa tgtattatcc gccattcctt gacggattta
     1141 aagaatacca tgtaacgcca ccatcacgag tgcagtcaat ccctgacacc ttacctttga
     1201 atgagctttt gataacacag cttttaggtg cagtatcaca ttgtgccttc aggattgaca
     1261 aaccatacaa caaacatgta gccatcatgg ggcaggggcc ggtaggattg ctatttacag
     1321 cattgatgaa aaattttggg gcacggagtg ttattgctat tgacccatta gactaccgtt
     1381 gtaaggctgc aataaacatg ggtgcaaccc ataccataaa tccccatacc agcgaccttt
     1441 tagaagcaat caaagctatt actgatggtt caatggctga tattgttatt gacgcatacg
     1501 gacaggatgt ggcagttatt aataaatgct ttgagatagc ccgtcacaat ggtcaggtag
     1561 ctttttttgg aatatgtctg gaagaagctc cccgccttaa cttcaatacc tttttcagaa
     1621 aagagctgcg catgattgct tctgttgggc ccgacctgac aatggattat ccctacgcac
     1681 tggatatgat tttaaaaggt gctattgatg ttacaccact gttaacacat gatattcctt
     1741 ttgaagatat tcaaaaaggt tttgaaatgg caagcaatag agaggacggc gcaataaaaa
     1801 ttgtattaag cttttaacaa taacgagtta taacagacga ggtacactat gccacgggca
     1861 aaaatatatg ggtatatagc agtgatagtt gctgcactat tgggatgtgc atttgtatat
     1921 tatgcattta ttcattacga tatgtatgca agtgttcact ttgattctat tgacacgggt
     1981 gcatattggg ctgttgtttt accaatagct ttagtaaccc tgtttgtttt agcaacaggc
     2041 ttctgggttg gatatactat tttaacaata aaggtggctc ctcctatgcc agaaataatt
     2101 gaaaaaaagg acaattcaaa gtttaaagca tttttgttat gtttggttac tatcgcactt
     2161 gctgtgtggt ttgtatatgg gttataccat cattcgtatt tagccattgc tattccggct
     2221 gcatgtatca cgcttgttat acttggcatg gtcttctggg ttggtattgc cataattact
     2281 acacgatcaa ctttaaagaa caataaatag caatttatgc gtgcactttt tctgtgttac
     2341 cgtggcaacc ctttttgtgg gggacagggt atatatttat acaatctcac aaaggaatta
     2401 gcaaaattag gcgttgcaat tgatgttatt gtaggacctc cctaccctga cccacttgat
     2461 gaatgggcta cagtctataa gctggaaaat ctaaatatgt gggcaatcaa aacaaaacat
     2521 ttcccatatg aaaagctaat taaactatat acgccatggc attttgtaga ttattgttta
     2581 acccgttttc acatattccc tgaaatggaa accttcagca tgaaagcgtt ctttttgcta
     2641 cgaaagcttt taaagacaaa ccattacgac attattcacg atgtacagtg tttagggtgg
     2701 ggattattac ccatgaaagg ttacggcatt cccattgtat ccacagtaca tcatccttta
     2761 acaaaggatc gcgaggcaga ttttcttgtg gacaataccg tatgggagat ggcatgtacc
     2821 atactctttt accctttaac catgcagcgt atcgttataa atcggctgga cagggtaata
     2881 acctcatcaa aagaaggcat taatgcactc aatgaagctt tccacctgcc acctgaaaag
     2941 gtatcggttg ttgataatgg gatggatgtt gaatttttcc gtaataatgg taaccctcgt
     3001 aaggaaaata cattactttt tgttggcaat actgaagacc ataaaaaagg gttgcgttat
     3061 ctttttgatg ccatgacgat gttacctgaa catgttacac ttaccatagt tgatgatgga
     3121 ccccctaaac gtttaacagc atggggttgg ataacagagc gtggtcttga aaagcgggta
     3181 acctttaccg ggaaggttga tttgcctaca ttagttcatc tgtacagcac cagcactatt
     3241 cttgtcatgg cttcgttgta cgaaggattt ggattacctg ctgcagaggc aatggcatgt
     3301 aatacgcccg tggtggtaac cagcgcaggg gcattacccg aggtagttgg cgattgcggc
     3361 gtgatagtac cacccagaga tgctgttgcc ttaaaagaag gcattgtaag gcttcttaac
     3421 aataaggcac tccgcgaaga attgggcaaa aaaggaagga aacgcgttga ggataacttt
     3481 gcatggccag ttgctgcaaa aaatacgctt gctgtgtatc atgatgttat ccagtcatac
     3541 aggagcagga tatgaaaata tgtttaatga gttatcgtgg caatccgtat tgcggtggtc
     3601 aggggattta cctgatgtac ctggcgcgcg aactggcaaa attaggacat gaggtccatg
     3661 ctattgttgg tccaccttat ccatttcccg ttgaaggtgt aacataccat tatgtcagta
     3721 accacaatta ttttaacata aaggattttg ttaaaccaca caagccattt gccactttta
     3781 accctttaaa cttttacgaa tttgtagcat caaagcttgg catattcccc gagatagaat
     3841 cattttcatt cagagcgtat agcaaattgc gacagttgct taaaactcac tcatttgatg
     3901 tcatccatga taatcagtgt ctgggatatg gatttttgct tataaagcag tttggcattc
     3961 cttttatatc caccatacat cacccgctta ctatcgaccg ttcaacatgg tttgaatatc
     4021 cttcaagctt cttccttaaa acaaagcgca tcctttatta cccgcttgtg atgcaaaaat
     4081 atgtagcaaa taacatggac cttattgtaa cggtgtcgca cgactctgca caggagattt
     4141 acaacgcatt tggagtgcca cttgaaaaac agcgtgttgt gtacaatggc atggacagct
     4201 ccatatttaa tccggtaaaa ctcccgaaaa ggcccaacag ccttatattt gttggcaatg
     4261 tagaagaccg caaaaaaggt gttatatata tgctgaaagc tttaacgctt acccgtcata
     4321 aggttcattt aaccattgtt gacggaggag cccccaaccg aaactatgtc ccacggtgga
     4381 ttgataaatt taatcttaat gaacaaataa cctttaccgg gaagatacca ctggataaac
     4441 tggttcaact gtatgcacaa acacagatag cactgtgccc ttccttatat gagggatttg
     4501 gatttccggc agcagaagcc atggcttgtg aattgccggt gatcgcaagc acaggaggtg
     4561 ctcttccaga agttgttgga caacacatga aaacaggaat actggtgcca ccacgtgacc
     4621 cgtatgcatt agccgaggct attgactttt tagtagaaaa tcctgatata cgaaaccgta
     4681 tagggaaagc cgcacgccag agggtattaa aaactttcac atgggaaaat gctgccaggc
     4741 agatggtaaa cgtatatcag gaggcaattg atgcttaccg ttgattttga tctccttgct
     4801 gttaaggata acaatattgc acttgatgca ggctgtggtc tggggcgcca ttcttttgaa
     4861 tttgtatcac gtggtgctaa tgttttttcc atggatatgg atatggattg cgtgcggcgc
     4921 actcgcttta cgttagtgta catgcgcgac aatgggcagg caaagcaggg tacccatttt
     4981 caggtattgt tggggaatgc gctgaagctc cctttcaggg ataacacctt tgaccgcgtt
     5041 atctgtgccg aggtcatgga acatgtgcct gatgataatc aggcatgtca tgagctggca
     5101 cgagtgttaa agccaggcgg gctgattgcc attaccgttc caacctatat ttccgaaatg
     5161 ctctacgaca ctttgaccta tgagtacttc acatcccccg gagggcacat ccgcaagtac
     5221 gtccccaaaa agctggcagc catcatggaa aataacgggc taaaaatcta tgcggttgat
     5281 ttccgtcatt cattccatac tatctactgg ctcatccgat gcgtggttgg
//
LOCUS       DAOOJH010000128         5179 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_12792_length_5179_cov_10.093677, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000128 DAOOJH010000000
VERSION     DAOOJH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12792_length_5179_cov_10.093677"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLJ75_11510"
     CDS             complement(<1..412)
                     /locus_tag="PLJ75_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPD05880.1"
                     /translation="MKEVVIVSAVRTPLGNFNGALSSMPATQLGAIVIEEAVKRAGIQ
                     KEDVDEVIMGQVLPCGYGQNPARQAAIIASMPWEVGCLTINKVCGSGLKAVMLAAQAI
                     QAGDAEVIVAGGMENMSMAPYYLEKARFGYRMGDS"
     gene            866..>1530
                     /locus_tag="PLJ75_11515"
     CDS             866..>1530
                     /locus_tag="PLJ75_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05881.1"
                     /translation="MNTTQHYNSALPFLFLGIVLVIVTVSCTSVEFVKTGNEYTPLPP
                     NSDVQVLLTVDKNQFDEIGIADIGGVSLPARIEKAKEIARSKGGDVIVPAIMCNEESK
                     KRMRSGYIIQSFYILKTRSTAIIQKPVEETRSFTQMVEKELLPEKRSATFQQLLREYP
                     QLHGEVFTNTMQPSGFYKIPPSLLVYGLSQHKLCLMKFDEENSALVFIPMDKINTIKA
                     SIEG"
     assembly_gap    1531..1630
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1631..>5179
                     /locus_tag="PLJ75_11520"
     CDS             <1631..>5179
                     /locus_tag="PLJ75_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPD05882.1"
                     /translation="IAQGNFTSIVSRRFVLIKERPNWPTLYYVSPQSAVLSHVGQGPQ
                     WTEIPTIYLGLNIFNVLTLDSTNTYDRAFYWLLKVEERAIETGYSHIEVYPPHVSVAL
                     NQLLDEVIRIATLSEVEFKEIPERKKIRPFTARNRESFLKMLNARNPDDSFDFEKNLK
                     AIRSLERLARRYKKGYDVASLREIVRLLVAASGHDIHEIRNSANILLERIFAPKEFDA
                     PLATSFINCANNSTHLFEFIIPEEGDYFVRIFSNAGSNQFPLEQDIEFFDIDCIYKEK
                     KGVYTARYQFTRIGSYDYCVYKKRKKRNQWLRYEGCSGRINVIPDVRGEIVLEIFPDI
                     HGHTRIYWMDQEHPGLVYNEHGEVIRLGRFSDITAHLEDLKERYHITALYLLGVQKRG
                     TNREDWAPEATSPSPFSPMSLTQIEDYLGGEEEFKELVAKAHAMDIKVIVDVVPHLNR
                     HSTELPDDYAVKCYDDSGNLVIRASTDGRYGSWNDGKLFNYRKLEVWEWLINSICTLI
                     DKYDIDGIRFDSGHAVPIMMKKNNYPFVYGQYRSLESLVEGEIIVNDREDGHFITTGY
                     YDCACRDTIAIPLHYLIMSSVAQTLKAKNKQFFIHIAECYWGHERYLSRSGIIPYNSA
                     LFKICEGIIHGTTDVREVYHFYDNYLPTALPPGTELLGILGNHDERRALNTFGQRGLR
                     AAIGLTIFLNNIIMDYEGSAEGESWKVYLDNIYVNWNQFEYVAHRSLESFYRQWYRFH
                     RSNKGKGYLIWANNHLVAASIKFTEDTIWIGVFNFADSSQNVALQFDNPALPITDESF
                     FKVVDPIYSPITKHYSYYTGKELKASKLYTVVSYTDRIKLLKLEPVDDITPLYDEFLS
                     DSLFRLYSISNPVHFKSNFMFCETIEHTASFDEFIHFLQTHIIPLFYPQYKNFIEIGF
                     KRILFYMFKFGFTHGNDVLKLIDNLSSHHDTTIAELGKSIQFHNRRGPIVFISAEAEP
                     FSKSGGLANVVYELPRELTKLGEEVIVITPKYRHGDDNAMKKMASALQRYKAAYTGKN
                     VRFMIENNTYEAGVHYAQVDGIHYYLFDHHEFFDGLYWGYTGEEKLRRRIAFARASAE
                     LITTFGLNPLFVLTNDAYTGIFNGIIRSDHIYYAHPNFKRTSFFHIIHNGGWQYFDSY
                     HRYENGKDLFSLFNLPHWRYNDFSDPTEYDKINCMAS"
BASE COUNT         1508 a         1104 c          995 g         1472 t
ORIGIN      
        1 gactatcgcc catacgataa ccaaaccggg ctttttctaa atagtagggt gccatactca
       61 tgttttccat cccaccagcc actatcacct cagcatctcc agcctgtatt gcctgtgctg
      121 caagcatgac agcttttaat cctgagccac acaccttatt gatggtaagg catcccacct
      181 cccacggcat gctggcaatg atggctgcct gccgcgcagg attttgtcca tacccgcagg
      241 gcaaaacctg ccccatgata acctcatcca catcttcttt ttgtatgccc gctcttttca
      301 ctgcctcttc tatgacgata gcacccaatt gtgttgccgg catggatgaa agcgctccat
      361 taaaattacc cagtggcgtt cgtacggcac ttacaatgac aacctctttc attaatcccc
      421 tccaggaata gattggtatt tcttttttag aaacatttca gtgcaccttt ccgtaactct
      481 gctgccattc cgggtttttt ccggaatctc tcctgccgct cctgtcattg cggacctgtt
      541 ccggaatctc ttttgccacc atgagatcct gaaaccagtt caggatgacg ttctacagtt
      601 caggacatgg tccatgatga ctgtcatcaa atattttcat cctctatata tgaacacaaa
      661 tcaagccatt atgcattccc caccgggaaa gtacggcgca ctaaaaaaaa gcacctgttg
      721 cccctatatc ccggccagat tatatgtcaa caattaatga tacatggtaa agttttattg
      781 aaaaagtcaa aaagtatttt tttcaattga ctatttttta ctgtttcact atgatttaaa
      841 tgaccacaat agtaaggaat tgattatgaa tacaacacaa cattataaca gcgcactacc
      901 atttttattt ctgggtatag ttttggtaat tgttacagta tcatgcacat ctgttgaatt
      961 tgtaaaaacc gggaatgaat acactccact cccaccaaac agcgatgtgc aggtattgct
     1021 caccgttgat aaaaatcaat ttgatgaaat tggcatagcc gatattggcg gtgtttcatt
     1081 acctgcccgt attgaaaagg caaaagagat agcacgcagc aagggtggcg atgttattgt
     1141 tccggctatc atgtgtaatg aagaatccaa aaaaagaatg cggagtggct atatcattca
     1201 aagcttctac attttgaaaa ccagaagtac agcaataata caaaaacctg tagaagaaac
     1261 cagatcattt actcagatgg tggaaaagga gttattgcca gaaaaacgat ctgccacttt
     1321 tcaacagctt ttacgggaat atccccaact gcatggcgaa gtatttacca ataccatgca
     1381 gccatccggg ttttataaga tacctccttc gcttttagtt tacggattgt cacaacacaa
     1441 gctgtgcctt atgaagtttg atgaagaaaa cagtgctctc gtctttatcc ccatggataa
     1501 aataaacact attaaggcat ccattgaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn catagcacag ggtaatttta catcaattgt tagccggcgt tttgtactta
     1681 taaaagaaag gccaaactgg ccaacactgt actatgtctc accacaaagt gctgtacttt
     1741 ctcatgttgg gcagggacca caatggactg aaattcccac tatctattta gggctcaata
     1801 tttttaatgt tttaacatta gatagtacca atacctatga tcgcgctttt tactggcttt
     1861 taaaggttga ggagcgagcc attgagacag gatattcaca tattgaggtg taccctcctc
     1921 atgtatctgt tgcccttaac cagctgcttg atgaagttat acgcatagcc accctttccg
     1981 aggttgaatt caaagaaatc cccgagcgga aaaaaatacg accatttacc gcccgcaacc
     2041 gcgaaagttt tttaaaaatg ctcaatgcgc gtaatccaga cgacagcttt gattttgaaa
     2101 aaaatctaaa agccatccgt tcacttgaac ggctggcacg gcgttataaa aaaggttacg
     2161 atgttgcatc actcagagaa atagtgcgct tacttgttgc tgcctcaggt cacgatattc
     2221 atgaaatacg caacagtgcc aacattttgc ttgagcgcat ctttgcccca aaggaatttg
     2281 atgctccact ggcaacatca tttatcaatt gtgcaaacaa cagcacccat ctatttgaat
     2341 ttatcatccc ggaagagggg gattattttg tacggatatt ctctaatgcc ggcagcaatc
     2401 aatttccgtt agaacaggat attgaatttt ttgatattga ttgcatttat aaagaaaaaa
     2461 aaggcgtata tactgcacgc tatcagttta cccggatagg atcctatgac tactgtgttt
     2521 ataaaaagcg gaaaaaaaga aaccagtggc tgcgctatga agggtgcagt ggcagaataa
     2581 acgttatacc tgatgttcgt ggagagattg tactggagat attccctgat atacacggac
     2641 atacccgcat ctactggatg gatcaggagc atcccggtct tgtctacaat gagcatggcg
     2701 aggtaatacg ccttgggcga tttagcgata ttacagcaca tctggaagat ttaaaggagc
     2761 gttaccatat aacagccctg tatctattag gtgtacaaaa gcgtggcact aaccgtgaag
     2821 actgggcacc tgaagcaaca tcaccatcac cattttcacc catgagttta acccagattg
     2881 aagactacct gggcggcgaa gaagaattca aggagcttgt agcaaaagcc catgcaatgg
     2941 atataaaggt catagtagat gttgtgcctc atctcaaccg ccacagcact gaacttcccg
     3001 atgactatgc agtcaaatgt tacgatgaca gcggcaatct ggttatccgc gcatccaccg
     3061 atggcaggta cggaagctgg aatgatggca agcttttcaa ttatcgtaaa ttagaagtct
     3121 gggagtggct tatcaacagt atctgcacac tcattgataa gtatgatatt gatggcatac
     3181 gctttgactc cggccatgcc gttcccatca tgatgaaaaa aaacaattac ccttttgttt
     3241 atggacagta tcgttcactg gagtcactgg tagaaggcga gattatcgtc aatgaccggg
     3301 aagatggcca tttcattact accggatact atgactgtgc atgcagggat accatagcta
     3361 ttccgttgca ttacctgatc atgtcttctg ttgcccagac gttgaaggca aagaacaagc
     3421 aattctttat tcatatagcc gaatgttatt ggggtcatga gcgctatctt tcacgcagcg
     3481 gtattatccc ctacaattcg gcacttttta agatatgcga gggcataatc cacggaacaa
     3541 ctgatgttcg tgaggtgtat cacttttatg ataactatct gcctaccgcc ctccctccgg
     3601 gaactgagct gctgggaatt ttaggtaacc atgacgaaag gcgcgcgctc aacacctttg
     3661 gtcaacgggg gctgagagca gctattgggc ttaccatttt tttaaacaat atcattatgg
     3721 actatgaagg aagtgctgaa ggcgaaagct ggaaggtata tcttgataat atttatgtaa
     3781 actggaatca gtttgagtac gttgcccaca gaagtctgga atcattttac cgacaatggt
     3841 atcggttcca tcgcagtaac aaagggaaag gatatttgat atgggcaaac aatcatttag
     3901 ttgctgcatc aataaaattt actgaagata ctatatggat tggtgttttc aattttgccg
     3961 atagttctca gaatgttgca ttacagtttg ataatcctgc attgcctatt accgacgaat
     4021 cttttttcaa ggttgttgat cccatctact cgcctattac aaagcattac agctattata
     4081 ccggcaaaga actcaaagca tcgaaactct acaccgtggt gtcctacact gacagaatta
     4141 agcttttaaa acttgagcct gtcgatgaca ttactcccct ctatgatgaa tttctgagtg
     4201 actcattatt tagattgtat tcaataagca atccagttca ttttaaatcc aatttcatgt
     4261 tttgtgaaac tattgagcat actgcttctt ttgatgaatt tatacacttt ttacaaacgc
     4321 acattatccc gctattttat cctcaatata aaaacttcat tgagattgga tttaagcgta
     4381 tactttttta tatgtttaaa tttggtttta cccatggcaa tgatgtttta aaattaattg
     4441 acaacctttc atcacatcat gataccacta ttgctgaatt gggaaaaagt atacaatttc
     4501 acaacaggcg tggacccatt gtatttatct cagcagaagc agaaccattt tcaaaaagcg
     4561 gtgggcttgc caacgttgtt tatgaacttc cccgtgagct tacaaagtta ggcgaagagg
     4621 taatcgtcat aaccccaaaa tatcgtcatg gggatgacaa tgctatgaaa aaaatggcat
     4681 ccgcattaca aagatacaaa gcagcgtata caggaaaaaa tgtacgcttc atgattgaga
     4741 acaacaccta tgaagccggt gtccattatg cacaggtaga tggtatccat tactatctat
     4801 ttgatcacca tgaatttttt gacgggcttt actggggcta cacgggcgaa gaaaaattgc
     4861 gacgtcgtat tgcgtttgca cgcgccagcg ctgagcttat aaccaccttt ggattaaacc
     4921 ctctttttgt attaaccaac gacgcctaca ccggaatatt caacggcatt atacgaagtg
     4981 accatatata ttatgctcat ccaaatttta aacgaacatc ttttttccat atcattcaca
     5041 atgggggatg gcagtatttt gattcatatc accgatacga aaacggcaaa gatctttttt
     5101 cactgtttaa ccttccccac tggcgataca atgatttttc tgatcccacc gaatatgata
     5161 agattaactg tatggcatc
//
LOCUS       DAOOJH010000129         5158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_12839_length_5158_cov_8.029786, whole genome shotgun sequence.
ACCESSION   DAOOJH010000129 DAOOJH010000000
VERSION     DAOOJH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12839_length_5158_cov_8.029786"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLJ75_11525"
     CDS             complement(<1..323)
                     /locus_tag="PLJ75_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD05883.1"
                     /translation="MVIDYMEEFKYSKETRTVILQAAKTEFAEKGYDGARLSLIAKRA
                     GVNQALIHYYFSNKEKLYLEVLQYLFHHGEISIIREHLKDVTLNPKEALFTAIYILVY
                     LHHNA"
     gene            850..2007
                     /locus_tag="PLJ75_11530"
     CDS             850..2007
                     /locus_tag="PLJ75_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPD05884.1"
                     /translation="MQPSNGIVYEFFIFCFKVASLIMRHKAHLKGKEYLKKIPHLVIF
                     APTHDSYFEVPSLSKVYYSLKPRPHFVVMAKKDFLSGNYLASNFGEKNKLLYYFFYFL
                     DKTGLPKAFFKKMKLIDIPRPFIEDFDNKNQVKHEIHSQMVKFKHSIHSGFSTLIFPE
                     GTTWSFGGLKNLRSGIFQLVDSTYRHFRQKLYILPINVKVDRCIAGKKDIFINVGEPF
                     FMLGDKEKFLDRVSEILKSLHTITFSQIAATYLQRMAMAVENIDKAIVLKKEELASQL
                     DTVINDIIVLVKEGKLPGIDEYLLDSNYRHKKLKRFLKFCIKKGYIEKIDGQYMINAQ
                     RVLASFHIKSYRKENPVGFTANEFLSINYNCIMTIYEKYCNNYKVITAPIQ"
     gene            complement(2013..2531)
                     /locus_tag="PLJ75_11535"
     CDS             complement(2013..2531)
                     /locus_tag="PLJ75_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05885.1"
                     /translation="MAKEHKNAPIICAVITGIALAGAGVGIWKSMPLIIALSIIPAIV
                     YEIYRTQGVFTTLASWLALIVTIAALYVIYKNTMIDIAPYISKFMTLPVNTKLIPVAY
                     VAPVVLVIIAVFLFKQTAGIYTRWLALVILLTSVALFYSLDPSFIKNLLSTPQVQQNI
                     KEGVKRGIRNIH"
     gene            complement(2558..3904)
                     /locus_tag="PLJ75_11540"
     CDS             complement(2558..3904)
                     /locus_tag="PLJ75_11540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPD05886.1"
                     /translation="MTVKNHSVKENYMPTTIKNLGPCTVVSPLAVKYRQREAAFINDA
                     IRVRFNAFIGKKPPSGGETIESFEAAGPREKIFFNPETTRVAITSCGGLSPGINDVIR
                     SLVMESYYGYGINDIIGIPYGYNGLNPEYNYTPIPLNPDNVKSIHMQGGSILGSSRGG
                     TRDMDKLVDTLQKLNVSILYTIGGDGTLRGSHDIAMIALQRGLPLAAVGIPKTIDNDI
                     SFIQRSFGFETAFSIAVEAIYAAHTEAIGAPNGIGLVKLMGRHSGFIAANAALASNDV
                     NFVLLPEVPFELYGANGLCAHLENRLQRRGHAVICVAEGAGQEILQKDSDKQYDASGN
                     VKLADIGAYLKEKIQEYFKQKKIEINLKYIDPSYMIRSAPAVPNDSIYCTLLGQNAVH
                     AGMAGKTDLVIGQWSNIYTHVPVEVAISKRKRIDPHSRFYSSVLDATGQPFAMINE"
     gene            4323..>5158
                     /locus_tag="PLJ75_11545"
     CDS             4323..>5158
                     /locus_tag="PLJ75_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HPD05887.1"
                     /translation="MAQKEDIVFPKGFLWGTATAAHQIEGGTTNNNWWLFEKEGTIKN
                     NDSALVACDHWNRFKQDFDLLKKLNCNAYRMSIEWSRIFPQPGTLDKKALDHYVTMIN
                     ELLKRKITPFVTLFHFTLPIWWCNEGGLLNRDKEHLEHFKQFCDVVAKTLKGKVKFYN
                     TINEPSIVMLGYITGTSAPGQKNMLKAIKALGTTMLMHAIAYHTIKSVDPDAKVGIVH
                     NVQITKPYNPNSIMDKLFTAFADYCFNGAIIRSLKKGKLPLQIFSSHRELINTNDFIG
                     VN"
BASE COUNT         1623 a          937 c          961 g         1637 t
ORIGIN      
        1 atggcattat gatgcagata taccagtatg tagattgctg taaaaagagc ctctttagga
       61 tttaatgtaa catctttcag atgttcccgg ataatgctga tttctccatg gtgaaacagg
      121 tattgcagca cttcaaggta tagtttttcc ttattggaaa aataatagtg gataagtgcc
      181 tggtttaccc ccgctcgctt agcaattaaa cttaaacggg caccatcata gcctttttca
      241 gcaaattccg tctttgcagc ctgcaatatg acagttcgag tttcctttga atatttaaat
      301 tcttccatgt aatcaataac catactttta agttgtcatc catgacatta atcaattatt
      361 ttaattaaaa aattaaattg attagttaaa tatatagcaa ttcttgtcaa tagcaaaata
      421 aaaaaataaa aaattttaaa aaaatattgt attataaatc aaacgataac acctatagaa
      481 tattatttta ggcaatttta gggtagttgc ttatggcaga gcattcatcc agccataagg
      541 gatgaacata tttagcgtct atcattctga gctgaagccg tctgtgtgga gcgaagtcga
      601 agaaaaaaat ctcactgtgg catggggatg tcattctgaa cgaagtgaag aatctctgct
      661 ttttatgaaa ggatagattc ttggggactg ggtctctcag aatgacacgg tattctattt
      721 taagaggaat gcaatcattt gaagatgaaa acaccatgct tttcattata ctttaatgta
      781 ttttttacta ttgacaattg ctggtgaata gtttattttt ataccagtaa agtggtttga
      841 ggtatgtata tgcagccatc aaatggcata gtctatgaat tttttatttt ctgttttaaa
      901 gttgcttcat tgattatgcg gcataaagct catttaaaag ggaaagaata tctaaaaaaa
      961 atcccccacc ttgtaatatt tgcccctacg catgatagct attttgaggt gccatccctt
     1021 tcaaaggtat attattcatt gaaaccacgt ccacactttg tggtaatggc aaaaaaagat
     1081 tttttaagtg ggaactatct ggcatcaaat tttggagaaa agaataaact gctctattat
     1141 tttttttatt ttttagataa aacagggttg ccaaaagctt ttttcaaaaa gatgaaactg
     1201 attgatattc cacgcccctt catagaagat tttgacaata aaaatcaggt gaaacatgag
     1261 atacactctc agatggtaaa gtttaaacat tctatacata gtggtttttc tacgcttata
     1321 tttccggaag ggacaacgtg gagttttgga ggtttaaaaa atttacgcag tgggatattc
     1381 cagcttgttg atagtaccta tcgccacttc aggcaaaaat tatatatact gccaattaat
     1441 gttaaggttg accgttgcat agccggtaaa aaggatattt ttataaatgt tggggagccc
     1501 ttttttatgc ttggggataa agaaaaattt ttagatagag tttctgaaat tttaaaaagt
     1561 ttacatacca taaccttcag tcagattgct gcaacatatc tccagcgcat ggcaatggcg
     1621 gttgaaaata ttgataaagc gatagttctt aaaaaagaag aattggcatc acagttagat
     1681 actgtcatca atgatattat tgtgctggtc aaagaaggga aactgcccgg tattgatgaa
     1741 taccttttgg atagtaacta tcgccacaaa aaattaaaac ggtttttaaa attttgcatc
     1801 aagaaagggt atattgaaaa gattgatgga cagtatatga tcaatgctca acgggtgctc
     1861 gcttcatttc atatcaagag ctacaggaag gaaaatcccg ttggatttac cgctaacgaa
     1921 tttttatcca ttaattataa ttgtataatg acaatatatg aaaagtactg taataattac
     1981 aaagtaatta cagcgcctat acagtaacag gtttaatgaa tattgcgtat cccccgtttt
     2041 acgccttctt tgatgttttg ctgaacctgc ggagttgaaa gtagattttt aataaatgaa
     2101 gggtccaggc tatagaaaag cgcaactgat gttaacagta ttacaagcgc taaccagcgg
     2161 gtatagatac ctgcagtttg cttaaacaaa aatacagcaa taatgacaag aacaaccggt
     2221 gcaacatatg caacaggtat aagctttgta ttaaccggca gtgtcataaa ttttgatata
     2281 tagggggcaa tatcaatcat ggtattttta tagataacat atagcgctgc aatggtaacg
     2341 atcagcgcta gccatgatgc caatgttgta aacacaccct gtgttctgta tatttcatag
     2401 acaatagctg gaataatact gagagctata ataagtggca tacttttcca tatgcctaca
     2461 ccagctcctg ctagtgcgat accggtaata actgcacaaa taattggtgc atttttatgt
     2521 tcctttgcca ttgcaaacct cctttagaat aaaattatta ttcatttatc atcgcaaaag
     2581 gttgtcctgt tgcgtcaagt acactgctat agaagcgcga atggggatct atgcgctttc
     2641 gttttgaaat tgcaacctca accgggacat gagtgtatat attactccat tggccaatga
     2701 ctaaatctgt tttcccggcc ataccggcat gaacggcatt ctgccctaat aatgtacaat
     2761 agatagaatc attgggaact gcgggggcac tgcgtatcat atagcttgga tcaatgtatt
     2821 ttaaattaat ttcaatcttt ttttgtttaa aatattcctg tatcttttct tttagatagg
     2881 caccaatgtc agccaatttt acattgcctg aagcatcata ttgtttatca gaatcttttt
     2941 gcagtatttc ctgtcctgcg ccttcagcga cacatatgac agcatgtcct cttctttgca
     3001 acctattttc aagatgggcg cataatccat ttgctccata cagttcaaag ggaacctcgg
     3061 gcaataatac aaaattgaca tcattgcttg ccagcgctgc atttgccgca ataaaacctg
     3121 agtgcctgcc cataagttta acaagtccta tcccgttggg tgctcctatt gcttctgtat
     3181 gcgctgcata gattgcctct acagcaatag aaaaagctgt ctcaaatcca aatgaacgct
     3241 gaataaagct tatgtcattg tcgatagttt ttgggatacc aactgctgcc aggggcaacc
     3301 ctctttgtaa tgcaatcatg gcaatgtcgt gtgagccccg cagtgtacca tcgccaccta
     3361 ttgtatagag gatgctgacg tttagttttt gcagtgtatc aacaagttta tccatatccc
     3421 tggtgccacc acgcgatgaa cccagaatgc ttccgccctg catatggatt gattttacat
     3481 tgtcaggatt taaggggatg ggggtataat tatattcagg atttaatcca ttataaccat
     3541 agggtatgcc aatgatgtca tttattccat atccataata cgattccatc accagtgaac
     3601 ggataacatc attaatcccc ggacttaatc ctccgcaact ggtaatagcg actcgtgttg
     3661 tttcaggatt aaaaaaaatc ttctcacgag gtcctgcagc ttcaaagctt tcaattgttt
     3721 caccaccaga gggaggtttt ttacctatga atgcattaaa acgaactcta atggcatcat
     3781 taataaaagc agcttcacgc tggcgatatt ttactgctaa tggagaaaca acggtgcaag
     3841 gtcccaaatt ttttattgtt gtcggcatat aattttcctt aacactatga ttttttaccg
     3901 ttataattcc aggtaaacgc aacacatttt gtcattctga gggacgaagt cccagaaaat
     3961 atactctttc acaaaagacg agattctttg ctgacgctca gaatgacata tccccacaac
     4021 ggcatgattc tttgtttgcg ctcaaaatgc tattgtcaca gcatataagt atggtaatga
     4081 atttattcaa tcacttttca taggtttttt acaatcactt ttttaccggt aactatcgcc
     4141 aatcatactc atttgttcca taattccata tatggtaatc tacattgctg ttgttcatag
     4201 agtatgtgta accacaatga taggttgctg tataataatt tttttattga tttttttata
     4261 gcaactcaat aatgtaaaaa agatgttttt atagtgtagt acaataataa agcgagggtg
     4321 ttatggcgca aaaagaagat attgtatttc caaagggatt tttgtggggc accgcaaccg
     4381 ctgctcatca gatagaaggt ggcactacaa acaacaactg gtggcttttt gaaaaagaag
     4441 gtacaatcaa aaataatgac agcgcgcttg ttgcctgtga tcactggaat cgcttcaaac
     4501 aggattttga tcttttaaaa aagctcaact gcaatgcata tcgcatgtca atagaatggt
     4561 cgcggatttt cccccagccc gggacgcttg ataaaaaggc acttgaccat tatgtgacga
     4621 tgataaacga gttgctgaag cgtaaaataa cgccttttgt gacattgttt cattttacac
     4681 ttcctatctg gtggtgcaac gaaggcggct tattaaacag agataaagaa caccttgagc
     4741 attttaaaca gttttgcgat gttgttgcta aaacattgaa agggaaggta aaattctaca
     4801 ataccattaa cgagccaagt attgtcatgc ttggctatat taccggtaca tctgctcctg
     4861 gacaaaaaaa tatgctcaaa gccattaaag cgctgggtac aacaatgctg atgcatgcca
     4921 ttgcttacca taccattaaa tctgttgacc ctgatgcaaa ggttgggata gtgcacaatg
     4981 ttcagatcac aaaaccgtac aatcctaaca gtatcatgga taagctattt acagcgtttg
     5041 ctgattattg ttttaatgga gctattatcc gctccttaaa aaagggaaaa ttgcccttgc
     5101 aaattttttc atcacacagg gagctaatca ataccaatga ttttataggc gttaattt
//
LOCUS       DAOOJH010000130         5027 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13248_length_5027_cov_10.021722, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000130 DAOOJH010000000
VERSION     DAOOJH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13248_length_5027_cov_10.021722"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1176)
                     /locus_tag="PLJ75_11550"
     CDS             complement(22..1176)
                     /locus_tag="PLJ75_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPD05888.1"
                     /translation="MPKKAFTTAKQKNDCIDSIINEMVNHNAFLILGHTNPDEDCVAS
                     MIAFALLARKFDKEVKIFTSKKIPQNFKYLVDIAVYNRIDVVMAIPRTFMADAIVICD
                     TPKPSMIDANRKVQSMLRNPDVRKIEIDHHIGADSDYAGDAGYCLVTGASSASELVGQ
                     IAIKLRAKKEILKKYVILDPLSRNLVLAILTGIIGDSQMGRYLKSSREKRMYRLFSNL
                     FNTILNTATIKEGNITNMDQIFEQLKQASENERRCFQYIKKKSKLYKWVGYAVLSEED
                     MHYLYDNFDDDDIISTTRALADNLAEDSGKIGMVAYYDTKDKSDLIQFRMRRSQHYKK
                     YDLRDFLKVMHIHNGGGHEGAIGFRLSKDAVNDYQHYVVAMVKKVNSLLP"
     gene            1514..>2226
                     /locus_tag="PLJ75_11555"
     CDS             1514..>2226
                     /locus_tag="PLJ75_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05889.1"
                     /translation="MKKTPIHIIVIFIAMGLIVPFSFAVQKDDDTPYKKGVKYFYQQK
                     FEIAELLFQEELKNNPENALAYSYLGDIFLYKKRYDGALQLYIKALELNPKSAEDAFR
                     IGQVHYYKKQPEEAINYFQKAFSLNPQLKFAYYHIGLTYLMLLRDKEKTIESWEKFLA
                     IAPEDPQYEKIKRVIELLRDPKFVLPPPGSDVSIEEALHLGGITLDEVQREAQDKKAG
                     HETKKTKNKLEEIYRDDEL"
     assembly_gap    2227..2326
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2656..5025
                     /locus_tag="PLJ75_11560"
     CDS             2656..5025
                     /locus_tag="PLJ75_11560"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD05890.1"
                     /translation="MAYYYDRYRNVRIAGIALCIILLLFSGYYLYTKLSKSSLQDIVD
                     IRQKQIELYEKLLETNPSDVEALIKLGNLYKEIGNIGKAIGYYNKALTIEPHNYNALN
                     ELGHAYALQGDYENAIKTLELAKKYFPKYPQAYNKLGNVYDTQEKLEKALKEYKSAIA
                     VAPAYQESYWNIAKQRMKKGDYKGAEAILKKGIKANPNDAEGYYNLGNLYMHKKNYAK
                     AIPQYQKAIALSPATGKYYRALGDAYLAQNKIPEAIGAYSTALAKDPGDALSAEKLGD
                     IYASQGNWGKAVDYYRQALNYNSNDQNLKNKYANALSKLQKPVFAQNEPSPVTIASNE
                     KNDTPAQALVRDKDLSDAAQQPSSVNAVNESALKWRELGDKYRAEKKYDEALKAYAKA
                     TQLDKTDDYSHHWMGRIYYHNHIDDEAKKSFQNSIIANPKNADSHYHLGLIYYTEGKY
                     NAAVIEFNKAIEEKPVFPKAYYARGLAYYKMNNLQTAIKDLKQSIEQDPNLDRAYFNL
                     GDIYLKQKNYKVALDYFTKDRELRPNNPDTLFKIAETYHEMKSYSNAETFYNKTLEAD
                     PEYFQALFNLGLISATRQNYAQAVSLYTRALSIKNDDPPTLYELGKAYEGLGDDNKAI
                     EYYSLAITKNPSYTKAYINLGNLYKKNKLHDKAIEQFKQAAINEPKSFEAHYNLANAY
                     STASMWDNAIEEYQTAIDLSPMNDQAHFFLGVAYREKGMYEQAANEFERSLQINSSLA
                     DAHEELGMLYYRKLKNNDKAIYHFEKLLSMKPNHPKADQIRDIIAMLKK"
BASE COUNT         1683 a          924 c          890 g         1430 t
ORIGIN      
        1 atagaataaa actatggtat atcatggcag caagctgtta acctttttta ccatagctac
       61 aacatagtgt tgatagtcat taacagcatc tttagacaat cgaaaaccta ttgccccctc
      121 atggcctcca ccattatgga tatgcataac ctttaaaaaa tctctcagat catatttttt
      181 ataatgctgg ctacgtctca tcctgaattg tataagatct gatttatctt ttgtatcata
      241 ataagcaacc attccaattt tacctgaatc ttcggctaaa ttatctgcca gcgctcttgt
      301 tgttgatata atatcgtcat cgtcaaagtt atcataaagg tagtgcatat cctcttcgga
      361 taaaacagca tacccaaccc atttatagag cttactcttt ttcttaatgt actgaaaaca
      421 tctcctttca ttttctgaag cctgtttcaa ctgttcaaaa atctgatcca tattggtgat
      481 attgccttct tttatggttg cagtattcag gatagtattg aaaaggttgc tgaacagcct
      541 gtacatcctt ttttcacggg atgacttaag atagcgcccc atctgcgaat cgccaataat
      601 accggttaaa atagccagca caaggttacg tgaaagggga tctaatatta catatttttt
      661 taaaatctcc ttctttgctc gcagcttaat ggctatttgt cccaccagct cacttgcgga
      721 agaagccccg gtaacaaggc agtatcctgc atcgcctgcg taatcactat cagcacctat
      781 atggtggtca atctctatct tccgtacatc gggattacgt agcatgctct gtactttacg
      841 gttggcatca atcatggaag gcttgggtgt atcacagata actatcgcat cagccataaa
      901 agtccttgga atggccatta caacatctat acggttatat acagcaatat caacaagata
      961 tttaaaattt tgcggtatct tctttgaagt aaaaatcttc acctctttat caaatttcct
     1021 tgcaagcagg gcaaatgcaa tcattgatgc cacgcaatcc tcgtcaggat ttgtgtgtcc
     1081 taaaataaga aatgcattgt ggttaaccat ctcattaatg attgaatcaa tgcagtcatt
     1141 tttttgtttt gctgttgtaa aagctttttt aggcataggt attttttaaa atttttaatg
     1201 gcgctgatca taatacaatc ttatgaaaat gtcaacagca tattttttta ttttttccag
     1261 tacttgactc agggttgatt attggttatt ttttaaaggt aaggttttat tacattttca
     1321 ttatcctatt gagtattatt gcttttttat gaatcttatt ttgaggaggt acgatggatt
     1381 ttgatattaa gcgattttta cttgccataa cgtctttgat tattttcttt atttttgcca
     1441 tgcttttttg ttaatgtatg caatcgtttt tactcaatta gtacactgta aagtgacgat
     1501 atagtaaaca attatgaaaa aaacacctat tcacatcata gtcattttca tagcaatggg
     1561 gttaattgtg cctttttcat ttgctgtaca aaaagatgac gatactcctt acaaaaaagg
     1621 cgtgaaatat ttttaccagc aaaaatttga gattgcagaa cttctgttcc aggaggagtt
     1681 aaaaaataat cctgaaaatg cgcttgccta ttcatatttg ggcgatatct ttttatataa
     1741 gaagcgttat gatggtgcat tgcagcttta tataaaagcg ctggagttaa atccaaaaag
     1801 tgcggaggat gcatttcgca tagggcaggt tcactattac aaaaaacagc ctgaagaagc
     1861 tatcaattat tttcaaaaag cctttagcct taatcctcaa ctaaaatttg cctattatca
     1921 catagggctt acctatctta tgctgttgcg agacaaggaa aaaacaattg agtcatggga
     1981 aaaatttttg gcgatagctc ctgaagatcc acaatatgaa aagataaaaa gagttattga
     2041 attattgagg gacccaaagt ttgttttgcc gccgccagga agcgatgttt ctattgaaga
     2101 ggcattgcat ctgggaggaa taacgcttga cgaagtacag cgcgaagctc aggataaaaa
     2161 agcagggcat gaaacaaaaa agacaaagaa taaattagaa gagatatatc gtgatgatga
     2221 attatannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaac aaaaaagaca
     2341 aagaataaat tagaagagat atatcgtgat gatgaattat agatggttgg tttatatcat
     2401 agcaatgatg atttgatgaa gatagagtgt taccattatt ctaatgctca acaaaaaata
     2461 tcactgtagc atgtcattct gagcggagtg aagaatcgcg tcgttttatg gaatgataga
     2521 ttgttgggaa ctgtgccccc tcagaatgac aaaagtactg tttttttcaa aatggcaatg
     2581 tgcagacacc attatcaaat tgctcatgct ggatcaaaat gaaacagctc tattctattt
     2641 ttataggagt tcatgatggc atattattat gaccgttacc gtaatgtacg gattgcagga
     2701 atagctcttt gtatcatttt acttcttttt tcaggttact atctgtacac aaaattgtca
     2761 aagagttcgt tacaggatat cgttgatata cgccaaaaac agatcgaact atacgaaaag
     2821 ctccttgaaa caaatccatc tgatgttgaa gcactcatca aactgggaaa cctttataaa
     2881 gagataggca atattggaaa agctatcggc tactataata aagcgctgac aatagaacct
     2941 cataactaca atgcccttaa tgaattgggg catgcttatg cattacaggg tgactatgaa
     3001 aatgccatta aaacgcttga actggcaaaa aaatattttc caaaatatcc gcaagcctat
     3061 aacaaattag gaaatgttta tgatacccag gaaaaattgg aaaaagcact gaaagaatat
     3121 aaaagcgcca tagcggttgc acctgcgtat caggaaagtt actggaatat tgctaaacaa
     3181 cgcatgaaaa aaggcgatta caaaggtgct gaggctattc taaaaaaagg aattaaagcc
     3241 aaccccaatg atgcagaagg ctactataat ctgggcaacc tctacatgca caaaaaaaac
     3301 tatgcaaaag caatacccca ataccagaaa gcaattgcac tttcccctgc aaccggtaaa
     3361 tactatcgtg ctctgggcga tgcctacctt gctcagaaca aaatccctga agcaattggc
     3421 gcttattcaa ccgcactggc aaaggatccg ggagatgcac tttcagcaga aaaattaggc
     3481 gatatctatg catcacaggg aaactggggg aaagctgtag actattaccg gcaggcgctg
     3541 aactataaca gcaatgatca gaatctaaag aataaatatg ccaatgcact ttcaaaactg
     3601 caaaaaccag tatttgcgca aaatgaacct tccccggtaa ctatcgccag taatgaaaaa
     3661 aatgatacac cggcacaggc acttgtccgc gataaagacc tttctgatgc cgcacaacaa
     3721 ccgtcatcgg tcaacgctgt caatgaatcc gcgttgaaat ggcgtgagtt aggcgataaa
     3781 taccgtgcag aaaaaaaata cgatgaagca ctcaaggcat acgcaaaagc aactcaactg
     3841 gataaaactg atgattactc acatcactgg atgggcagaa tctactacca taatcatatt
     3901 gatgatgaag ctaaaaaatc attccagaat tcaattattg caaatccaaa aaatgctgat
     3961 tcacattatc atttagggct catctattac accgaaggaa aatataatgc ggcagttatt
     4021 gagtttaaca aagctattga agaaaagcca gtttttccaa aagcatacta tgcacgaggt
     4081 ttggcttact ataaaatgaa caatctgcag actgctataa aagatttaaa acaatccatt
     4141 gaacaggacc ctaacttaga cagggcatat ttcaatctgg gtgacatcta cttaaagcaa
     4201 aaaaattata aagttgcact ggactatttt actaaagaca gggagctgcg gcctaacaat
     4261 cctgacaccc tctttaaaat tgcagaaacc taccatgaaa tgaaatcata ttcaaatgct
     4321 gaaacatttt acaataaaac actggaagct gatcctgaat attttcaggc tctttttaac
     4381 ctgggactga tttcggcaac cagacaaaat tatgcgcagg cagtttctct ttatacacga
     4441 gctttatcca ttaaaaatga tgacccgcca acactctatg aactgggtaa agcgtatgaa
     4501 ggtctgggtg acgataataa ggcaatagaa tattattcac tggctatcac caaaaatccc
     4561 agctatacca aagcatatat taatttagga aatctttata aaaagaacaa acttcatgat
     4621 aaggcaattg aacaatttaa acaggctgcc atcaacgagc caaaatcatt tgaagcccac
     4681 tacaatttag ccaatgcata ttctacagca tcaatgtggg ataacgctat agaagaatac
     4741 caaaccgcaa ttgacctatc ccctatgaat gatcaggctc atttcttttt gggagtagca
     4801 tacagggaaa aaggtatgta tgaacaggct gcaaatgaat ttgaacgctc attgcagatt
     4861 aacagttcac tggctgacgc tcacgaggaa ttgggaatgc tatactaccg taagttaaag
     4921 aacaatgaca aagcaatata ccattttgaa aagcttttgt ccatgaaacc aaaccatcca
     4981 aaagcagacc agatacggga tattatagct atgttgaaga aataatg
//
LOCUS       DAOOJH010000131         4984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13390_length_4984_cov_6.778048, whole genome shotgun sequence.
ACCESSION   DAOOJH010000131 DAOOJH010000000
VERSION     DAOOJH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13390_length_4984_cov_6.778048"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLJ75_11565"
     CDS             <1..390
                     /locus_tag="PLJ75_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPD05891.1"
                     /translation="LLKDFSGIQYYEMGLSNAKGKINFCINGSSSKIDSSGTKIIYVD
                     RLDNIIFEPVQYIKMDVEGYESSVLEGCEKIIKDYQPSLAVCAYHKPDDLWKISEQVL
                     SINKSYKVYLRHYTEGITETVLYFLPD"
     gene            435..1181
                     /locus_tag="PLJ75_11570"
     CDS             435..1181
                     /locus_tag="PLJ75_11570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD05892.1"
                     /translation="MIINKLLSHLNNLSLSQLPDFINKMIDPDTFKRKEFIYKCSQES
                     PEGSYILDAGAGQCPYKGFFNKQKYIATDLSVGDINWDYSKLDVLSNLETLPFKNNVF
                     HSIICINVLEHVKEPFNVISEFYRVLKPGGSLYLTVPQGWWLHQEPYDYFRYTNYGIS
                     YLLEKAHFFINDIKPTSGYFSYLANRITFLPKALFWTIKSKIIRILLLPFEIISYLLF
                     VLLIPIILNCIDGYDKQNKFTLHYMVKASK"
     gene            1187..2530
                     /locus_tag="PLJ75_11575"
     CDS             1187..2530
                     /locus_tag="PLJ75_11575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HPD05893.1"
                     /translation="MYTEWTKKSWQNFNYYQKANWYDTVLLADVLQELSKLPALVFAG
                     ETRSLKKDLIDISRGDAFLLQCGDCSESFVDCQGPRIHNLIRIILQMSMIITYITNKR
                     VVKIGRIAGQYAKPRSNEFEIVDNVQLPIYRGDMINSFLPTQEYRKHDPKRILEAYYK
                     SAATLNLIRAFTKGGYASLQSITDWQKHYFKNFPIMDKYSEIVINITKAINFMKALGL
                     DVNNPQFNEVNFYTSHEALILEYEEALTRIDTTTGDWYDTSAHMLWLGNRSRHCDSSQ
                     VEFLSGINNPIGIKVGPDYNDEDLMCIIDKLNPANEQGKICIITRFGIPTINKQLPVL
                     IKHIKRKKNNVVWICDPVHGNTYVNKYNRKVRRFEDIVKEIESFWEICIHEKVIPGGI
                     HIELTGDNVTECIGGLRALSEEDLDNNYTSLCDPRLNAEQSVELAFKIADIVKQY"
     gene            2562..>3305
                     /locus_tag="PLJ75_11580"
     CDS             2562..>3305
                     /locus_tag="PLJ75_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD05894.1"
                     /translation="MKKLLIAGGSHSDIPLILAAKKLGYFVITTGTREEDLGHQYSDK
                     YIKADYSNMEQIVSIAIDEKVDALCPCCNDFSAITCSYVAEKLGFKTFDTLEVTRTIH
                     YKNKFREFMKQYNFPSPVAANFDNVEDALQSIEALQLPLIVKPVDLTGGKGISRIATY
                     DEAKQAIIKALSISRQKKIVIEEFIDGSYHGLSVFLKNKKVAFYFFDNEYYYLNKYLV
                     AGAYCPGSVPKQAIQQLCAVYQKMAEILSL"
     assembly_gap    3306..3405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3406..3599
                     /locus_tag="PLJ75_11585"
     CDS             <3406..3599
                     /locus_tag="PLJ75_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05895.1"
                     /translation="KKIVYSHEINNNILEKFEWYTTGETVTDYLTHKFGIVFLKFDSY
                     NNMVDCINSINELIKVEME"
     gene            3647..4795
                     /gene="rffA"
                     /locus_tag="PLJ75_11590"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3647..4795
                     /gene="rffA"
                     /locus_tag="PLJ75_11590"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HPD05896.1"
                     /translation="MDTHNIFIPFNKPPHTGNEEQYIKQALQSGKISGDGKFGNMCEQ
                     WFQSQLQCKRTLLTSSCTHALEMCAMLIDTKPGDEIIMPSYTFVSTANAFVLRGATIV
                     FVDIRPDTMNIDETKIEKAITTKTKAIVVVHYAGVACAMDAIRNIAMKYNIPVIEDAA
                     QAMLSTYNGSHAGTLGQLGTFSFHESKNYTSAGEGGLLLVNHEQWIQRSEIIREKGTN
                     RSQFFRGMVDKYTWVDIGSSYLMNDVSAAYLWGQLEKADDIFHNRMKTWNMYYESLLP
                     LNTKGIIALPIVPDECRHNAHMFYIKVDDLHVRTQLLKYLKNNGIYAVFHYVPLHSSP
                     AGQQYGRFDGTDEYTTKESERLLRLPLYYGMPEQHCKKVIDTIYQFFS"
BASE COUNT         1722 a          818 c          897 g         1447 t
ORIGIN      
        1 ctgttgaaag atttttcagg aatacagtat tatgaaatgg gattgtcaaa cgctaaaggt
       61 aaaataaatt tttgtatcaa tgggtcgagt tcaaagatag attctagtgg tacaaaaata
      121 atttatgtag ataggcttga caatatcatt tttgaacctg tgcagtatat aaaaatggat
      181 gttgaaggtt atgagtcaag tgtactggaa ggttgtgaaa aaattataaa agattaccaa
      241 ccgtcattgg cagtgtgcgc atatcataaa cctgatgatt tatggaaaat ttctgagcag
      301 gttttatcca ttaataaatc atataaagta tatttacgcc attataccga agggattacc
      361 gaaactgttt tatatttttt accagattaa tttgtaacaa caatgttatt attaaaattc
      421 aaagttggct tgctatgatt attaataaat tactatcaca cctaaacaat ctatctttat
      481 cacagcttcc tgattttata aataagatga tcgatcctga cacctttaaa cgaaaagaat
      541 ttatttataa atgctcacag gagtcaccgg aaggatcata tatccttgac gcaggtgccg
      601 ggcagtgccc ttataaaggc ttctttaata aacaaaaata tattgccact gaccttagtg
      661 taggcgatat aaactgggac tatagcaaat tagatgtatt gagtaatctt gaaacattgc
      721 catttaaaaa taatgtcttc cacagtatta tttgtataaa tgttttagag catgtaaaag
      781 aaccattcaa tgtaatttct gaattttata gagttttaaa acctggcggt tcattgtacc
      841 ttactgtacc gcagggatgg tggttgcatc aagaaccata cgattatttt cgctatacaa
      901 attacgggat atcatacttg ctggaaaagg cccatttttt cattaacgat atcaaaccaa
      961 catcaggtta tttcagctat ctggcaaaca gaattacctt tttgcctaaa gctttgttct
     1021 ggacaataaa aagcaaaata atacgaattt tgctgctacc ttttgaaatc atttcatatc
     1081 ttttatttgt actgcttatc ccaataattc ttaattgtat cgatggttat gataaacaaa
     1141 ataaatttac actacactac atggtaaaag catctaaata aaaagtatgt atactgaatg
     1201 gacaaaaaaa tcatggcaaa attttaatta ttaccaaaaa gccaattggt atgacaccgt
     1261 acttttagca gatgttttac aggaattaag caaactgccg gcattggtat ttgctggtga
     1321 aaccagatct ctgaaaaaag acttaattga catatcaagg ggagatgcat ttttgctaca
     1381 atgcggtgac tgttctgaaa gttttgtaga ttgtcaggga cccagaatcc ataatctcat
     1441 acgtattatc ttacagatgt ccatgatcat aacttacatc acaaataaaa gagtggttaa
     1501 aattggaaga attgcgggtc aatatgcaaa accacgttca aatgaatttg aaatagtgga
     1561 taatgttcaa ttgccaattt acagaggtga tatgatcaat agctttttgc ccacacagga
     1621 atatcgtaaa catgacccaa aaaggatatt ggaagcttat tataaatcag cagcaacatt
     1681 aaacttaata agagcattta caaagggagg atacgcatcc cttcagtcaa tcacagattg
     1741 gcaaaagcat tacttcaaaa attttcctat aatggataaa tattctgaaa ttgttataaa
     1801 cataacaaaa gcaattaact ttatgaaagc attggggctg gatgtaaata atcctcaatt
     1861 taatgaagta aacttttata catcacacga agctttaata cttgaatatg aagaagcact
     1921 tacaaggatt gatacaacca ccggtgattg gtatgatacc agtgctcata tgttgtggtt
     1981 aggcaacaga agccgtcatt gcgatagttc tcaggttgaa tttttgagtg ggatcaataa
     2041 tcctattggt attaaagttg gcccggatta taacgatgaa gatttaatgt gtataattga
     2101 taaacttaat cctgctaatg aacagggaaa aatatgcatt attactcgct ttggtatacc
     2161 tacaataaac aaacaacttc cagttttaat aaaacatatt aaacgcaaaa aaaataatgt
     2221 ggtatggata tgcgatccag ttcatggtaa tacgtatgtt aataaatata atcgcaaagt
     2281 gcggcgtttt gaagacatag taaaagaaat tgaatcattc tgggaaattt gtatacatga
     2341 aaaagttatc ccgggaggca tccatataga attgactggc gataatgtaa ctgaatgcat
     2401 tggtggattg cgtgctttat cagaagaaga tttagataac aactacactt cattatgcga
     2461 tccaaggctt aatgcagagc aatctgttga gttagccttt aaaattgctg atattgtaaa
     2521 acaatattga tgcttatagc ttaaaaatca ggaatcaaac aatgaaaaag ttattgatag
     2581 caggaggaag ccattcagat attccattaa ttttagcagc aaaaaaattg ggctattttg
     2641 ttattactac tggcacgcgt gaagaagatt taggacacca atactctgat aaatacatta
     2701 aagcggatta ctcaaatatg gaacaaatag tatcgatagc cattgatgag aaagttgatg
     2761 cgctatgccc ctgttgcaat gacttttcgg caataacctg ttcgtatgtt gctgaaaaac
     2821 ttggctttaa aacatttgat actcttgaag taaccagaac aattcattac aaaaacaaat
     2881 ttcgcgaatt tatgaaacaa tataattttc catcgcctgt tgcagcaaac tttgataatg
     2941 ttgaagatgc actgcaatca atagaagcat tacaattgcc gctcatagta aagccggttg
     3001 atttaacagg agggaaaggt atatccagaa tagcaacgta tgatgaagca aaacaagcca
     3061 ttattaaagc actatctata tcccggcaaa aaaaaatagt aatagaagaa tttattgacg
     3121 gatcatatca tggactttcg gtgtttttaa aaaataaaaa ggtagccttt tatttttttg
     3181 acaacgaata ctattactta aacaaatatc ttgttgctgg cgcatactgt ccaggaagtg
     3241 tacccaaaca agctattcaa cagctttgcg ctgtgtatca aaaaatggcg gaaattcttt
     3301 ctttannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntaaag aagatagtct
     3421 atagccatga aataaacaat aacatattgg aaaaatttga atggtacaca accggcgaaa
     3481 ctgttacgga ttatttaact cacaaatttg ggattgtatt tttaaaattt gattcataca
     3541 ataatatggt tgactgcatt aatagtatta atgaacttat taaagttgaa atggaataga
     3601 tagtagtata ttaactacga taacaataat aaaataattt aaaactatgg atacccataa
     3661 catctttatc ccctttaata aacctccaca taccggcaac gaagagcaat acataaaaca
     3721 ggcattgcaa agcggtaaaa tttctggcga tggcaaattt ggcaacatgt gcgagcaatg
     3781 gttccaatcg caactgcagt gtaagcgcac actgcttacc tcttcatgca cccatgcatt
     3841 ggagatgtgt gcaatgctca ttgatacaaa gcccggagat gaaatcataa tgcccagcta
     3901 tacctttgta agcaccgcca atgcctttgt actgcggggt gcaaccattg tatttgttga
     3961 tatacgtccc gataccatga acatagatga aacaaagatt gaaaaagcta taaccacaaa
     4021 aacaaaggca attgttgtgg tgcattatgc aggcgttgca tgcgccatgg atgcaatacg
     4081 taacattgcc atgaaatata atataccggt tattgaagac gcagcacagg ctatgttgag
     4141 cacctataac ggtagccatg ctggcacact gggacaactt ggaacattca gcttccatga
     4201 aagcaaaaat tataccagtg caggcgaagg cggattactg cttgtaaacc atgaacaatg
     4261 gattcaacgt tcagagataa tccgcgaaaa gggtaccaac cgcagtcaat tttttcgggg
     4321 gatggtggat aaatatacct gggttgatat tggctcaagc tatcttatga atgatgtcag
     4381 cgctgcatac ctgtggggac aactggaaaa ggcagatgac attttccaca acagaatgaa
     4441 gacatggaac atgtactatg aatcgctgct gccgcttaac acaaaaggca taatagcgtt
     4501 gccgatagtt cctgatgaat gcaggcacaa tgcgcatatg ttctatatca aggtagatga
     4561 tttgcacgtt aggacgcagt tgcttaaata tttaaaaaac aatggcatat atgcagtatt
     4621 ccattatgtg ccgttgcaca gctcccctgc cggacagcaa tacggaagat ttgatggcac
     4681 tgatgaatat accacaaaag aaagtgagcg tctactgcgc ctgcccctgt attatggaat
     4741 gccagagcaa cattgcaaaa aggttataga taccatctat caattttttt cataaaaccg
     4801 tctgctttat aaaaatgtaa cactatttac attctgaaga cacgcagtac ttgtgtcaga
     4861 gcatactatc cttgaaggta cacctataca ttcaatactg acaaataggt taccatgggg
     4921 tagtacctag tatacccttt acaatacctg ccaatagctg tacaatggtt atatgttggt
     4981 tttt
//
LOCUS       DAOOJH010000132         4950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13491_length_4950_cov_8.099285, whole genome shotgun sequence.
ACCESSION   DAOOJH010000132 DAOOJH010000000
VERSION     DAOOJH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13491_length_4950_cov_8.099285"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLJ75_11595"
     CDS             complement(<1..190)
                     /locus_tag="PLJ75_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPD05897.1"
                     /translation="MDYKDLNVWKEALGLVEDVYTVIETMPKTELYGLSGQIKRSCIS
                     IPSNIAEGANRQTKKEFIQ"
     gene            complement(253..780)
                     /locus_tag="PLJ75_11600"
     CDS             complement(253..780)
                     /locus_tag="PLJ75_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05898.1"
                     /translation="MERLSVLKNIITILKKKDIKLSKILIQKVIFFIKELYPNHGFGY
                     RFEMYKYGPYSHELSSDLDILKIQGEIDIKGNEILINNDNDVIDNDRLTSFVANTLLP
                     LFKDNDDFNNIELLGTVLYCIKALQENGLKLNKESVIQEVKGWKGTKYDDTVIGKAYN
                     RVINLVDSGWLLVDS"
     gene            complement(821..2116)
                     /locus_tag="PLJ75_11605"
     CDS             complement(821..2116)
                     /locus_tag="PLJ75_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD05899.1"
                     /translation="MSEDLLHKINLKHRMRDPLYGFIWLTQEELEIIDTPIFQRLRRI
                     SQLALTKYVYPSAEHSRFVHSLGVLQCATNIFLELVRNSEDLNKKDDLDILLKKLRFA
                     ALLHDIGHLAYSHASEQTLLGDTLKHEDVSKYIIQNYEPIKHVLLKCFNDATILNLIS
                     SLVAGKVIKEYDLLKKIISGVFDADRADYLIRDSYICGVKYGQFDVERFIGSFIVKNG
                     QYCIRYEHLNAVELMVFARYQYNMQVPFHRTRQGYDIVLSKFIEKQKENIQTGIQKQK
                     GEIVTIDYDTFVKFDDYYVFELIKKFAGEDNFAKILMRDKAKLHPVFDYTCNAENAYY
                     KKLYEQFIKGLISEGLEENVSFFKTVKNVKIYDLLKISDEQNESDYKVLKDDKLRDLF
                     QMSPVLFGNTNANEMIIYRVYVDDNCIGTAKKVLQDVIE"
     gene            complement(2279..>2609)
                     /locus_tag="PLJ75_11610"
     CDS             complement(2279..>2609)
                     /locus_tag="PLJ75_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD05900.1"
                     /translation="LWGTGSPYREFMHVDDLASACIFLMDKYDFAEIGEFVNIGTGVD
                     MPIKELATLIKDIVGFNGNIVWDTTKPDGTPRKLLDVSRIKALGWKPEIGLEEGIRMV
                     YGGMGNS"
     assembly_gap    2610..2709
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2710..3441)
                     /locus_tag="PLJ75_11615"
     CDS             complement(<2710..3441)
                     /locus_tag="PLJ75_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD05901.1"
                     /translation="MDNTSCIAVLGASGMVGNAITRKLINSNYRTIIATYNKNNITFD
                     SNAVKTVQLDATNQNEVERFFKEYNPEYVFLAAAKVGGILANNTYKAEFIYNNMAIAL
                     NVIHSAYKYGVKKLLNLGSSCIYPKLAPQPLKEEYLLTGPLEPTNEPYAIAKIAAIKL
                     CRYYNEQYGTNFISVMPTNLYGPHDNYNLETAHVLPALLRKFHLAKLLEQGDFEGLAR
                     DLRTYPIGFGLSLPASATRQDMLSIL"
     gene            complement(3480..4619)
                     /gene="gmd"
                     /locus_tag="PLJ75_11620"
     CDS             complement(3480..4619)
                     /gene="gmd"
                     /locus_tag="PLJ75_11620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD05902.1"
                     /translation="MSDVALITGITGQDGAYLAEFLLNKGYVVHGIKRRASSFNTDRI
                     DHLYVDPHTDGAAFFLHYGDLTDSTNVIRIIQQVQPDEIYNLGAQSHVQVSFETPEYT
                     ANSDALGTLRILEALRILGMEKTVRFYQASTSELFGQVQDVPQKETTPFYPRSPYGVA
                     KLYAYWITVNYREAYNMFACNGILFNHESPLRGETFVTRKITRGLARICLGLQDCLYL
                     GNVDAKRDWGFAGDYVKAMWLILQHDKPDDYVIATGNTHTVRQFVELAAKTVGLTLVW
                     HGRGVDEMGIVDSVGTVGIAGIDKLGNEKLIGKPIVKIDPRYFRPTEVDILLGDATKA
                     KQTLGWTPEVSFETLVDMMMAHDVNDQLKNVHLKNGGFDVKNYCE"
     gene            complement(4681..4824)
                     /locus_tag="PLJ75_11625"
     CDS             complement(4681..4824)
                     /locus_tag="PLJ75_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05903.1"
                     /translation="MRCKLQGVQLCSENVFVAIVILNGVHAILKPQAKDIAFIKKNRF
                     SGH"
BASE COUNT         1463 a         1119 c          675 g         1593 t
ORIGIN      
        1 attgtataaa ttcttttttt gtttgcctgt ttgctccttc tgcaatatta gatggtattg
       61 atatacacga acgctttatt tgtcccgata aaccataaag ttcagttttt ggcattgttt
      121 ctattactgt ataaacatcc tcaactaaac caagtgcctc tttccaaaca tttaaatctt
      181 tataatccat ccacaaactc ccatcaacta acaactatcc cccatcaact atcccccatc
      241 aactatcccc catcaactat caactaacaa ccatccacta tccaccaaat taataacccg
      301 gttatacgct ttcccaataa cagtatcatc atactttgtt cctttccatc ccttcacctc
      361 ttgtattaca gattctttat tcaacttcaa cccattttct tgcaaagctt ttatacaata
      421 taaaactgtt ccaagaagct caatgttatt aaaatcatca ttatctttaa acaaaggcag
      481 taatgtatta gccacaaaac ttgtaagcct gtcattgtca attacatcat tgtcattatt
      541 aattaatatc tcatttcctt taatatcaat ttctccctga atttttaata tgtccagatc
      601 agaagaaagt tcgtgtgaat acggaccata tttatacatt tcaaatctat atccaaatcc
      661 atggtttgga tataactcct tgataaagaa tattaccttt tgaattaata tctttgatag
      721 tttaatatct ttttttttaa gtatagttat tatatttttt aatacgctta atctttccat
      781 tgcaacctcc cctgtttttt atattttttt ctataatgtt ttattcaatc acatcctgca
      841 atactttttt tgccgtacca atacaattat cgtccacata aaccctataa ataatcatct
      901 catttgcatt agtgttacca aataaaacag gcgacatttg aaataaatct ctaagcttgt
      961 catcctttaa cactttataa tcagattcat tctgctcatc acttatcttt aaaagatcat
     1021 aaatttttac atttttaact gttttaaaaa aactaacatt ttcttctaat ccttcactta
     1081 taagcccttt tataaactgc tcatacaact ttttataata ggcattttct gcattacatg
     1141 tataatcaaa tacagggtgt aactttgctt tatcccgcat caatattttt gcaaaattat
     1201 cttctcctgc aaattttttt atcaactcaa atacataata gtcatcaaac tttacaaatg
     1261 tatcataatc aatagttact atctcccctt tttgtttctg tattcctgtt tgtatgtttt
     1321 ctttctgttt ttcaataaat ttagacagta caatatcgta tccctgcctt gtcctatgaa
     1381 atggtacctg catgttgtat tgatatcgtg caaaaaccat aagctcaaca gcattcaaat
     1441 gctcataccg tatacaatat tgaccatttt ttacaataaa gcttccaata aatctttcaa
     1501 catcaaattg accatactta actccacaaa tatatgaatc ccttataaga taatctgccc
     1561 tatcagcatc aaatacacct gatataattt tttttaacaa atcatactct ttaataacct
     1621 taccggcaac taaactgctt atcaggttca aaattgttgc atcattaaaa cattttaata
     1681 ggacatgttt tattggttca taattttgta ttatatactt gcttacatct tcatgcttta
     1741 atgtatcacc aagcaatgtt tgttcagatg catgcgaata agccagatgc cctatatcat
     1801 gtaatagagc agcaaaccgc aactttttta atagtatatc taaatcatct tttttattta
     1861 aatcttcaga atttctcact aattctaaaa atatatttgt agcacattgt aatacaccta
     1921 acgaatgaac aaatcgtgaa tgttctgccg aaggatatac atatttagtc agtgcaagct
     1981 gagatatcct tctcaacctc tgaaaaattg gagtgtcaat tatttccaat tcttcctgtg
     2041 taagccatat aaaaccgtat aaaggatccc gcatcctatg tttaaggttt attttatgga
     2101 gcaaatcttc tgacatagct tatttcaaat aaaaaaacat aaaatcaatg taaaccacaa
     2161 atgcctgcgt gtaatgtaaa agcccgtatt gtgcagaccc taaataccgt gctacctatc
     2221 ttacatctta catctatcaa ccatccccca tcaactatca actatcaact atcaaccatc
     2281 aactattccc catcccgcca tacaccatcc ttatcccttc ttccagtcca atctctggtt
     2341 tccagcccaa tgcttttatt ctgcttacat ccagtaattt ccggggtgtg ccatcaggtt
     2401 ttgttgtatc ccaaacaatg ttgccgttaa aacctacaat atccttaatt aaagtggcaa
     2461 gctcttttat gggcatatcc acaccggtgc caatatttac aaactcgcca atttccgcaa
     2521 agtcatactt atccatcaaa aatatgcagg cgctggctaa atcatccaca tgcataaact
     2581 cgcggtatgg agagcctgtg ccccataaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnng aggatggata gcatgtcctg tctggttgca cttgcaggca gggaaagccc
     2761 aaaacctata ggatacgtcc gtaaatctct ggctaaacct tcaaaatctc cctgctcaag
     2821 aagcttagca aggtggaatt ttcttagtaa agccggtaat acatgagcgg tttccagatt
     2881 atagttgtca tggggaccat aaaggttagt tggcataacc gaaataaagt ttgtgccgta
     2941 ctgttcattg taatatctgc acagttttat agctgcaatc ttggcaatgg catagggctc
     3001 attggttggc tcaagaggac ctgtcaggag atactcctct tttaaaggtt gaggagcaag
     3061 ttttggataa atgcatgaag aaccaagatt taagagtttt ttaactccat atttataagc
     3121 actgtgaata acattcagcg caatagccat gttgttgtat ataaattctg ccttataggt
     3181 attgttggca agtatacccc ccacctttgc tgctgctaaa aatacatact ctggattata
     3241 ttctttaaaa aaacgttcaa cctcattttg gtttgtggca tcaagctgta cggttttaac
     3301 cgcattgcta tcaaaggtta tattgttttt attgtaggtt gctattattg tgcgatagtt
     3361 actattaatg agctttctgg ttatagcatt acccaccatg ccactggcac ccagtacagc
     3421 aatacagctt gtattatcca tacaacacct catcaacagc catatgctgt gaatatagtt
     3481 tattcacagt aatttttaac atcaaatcct ccgtttttta aatgcacatt tttcaactgg
     3541 tcattgacat catgcgccat catcatatca accagcgttt caaacgatac ttcaggcgtc
     3601 catcccagcg tttgttttgc ttttgtggca tcacctaata gtatatcaac ttcggtgggt
     3661 ctaaaatatc gtggatcaat cttcactatg ggcttcccaa taagtttttc attgcctaac
     3721 ttatctatgc cggcaatgcc caccgtgcca acgctgtcaa caatccccat ttcatccacg
     3781 cccctgccgt gccataccag ggttaagcca accgtctttg ccgctagttc aacaaactgg
     3841 cgtaccgtat gggtattccc tgttgctatc acataatcat caggcttgtc gtgttgcaaa
     3901 ataagccaca tggcttttac atagtcacct gcaaagcccc agtcgcgctt ggcatcaaca
     3961 tttcctaaat acagacagtc ctgcaagccc agacatatcc gtgcaagccc acgggtaatc
     4021 ttgcgtgtaa caaacgtttc accccgcaat ggcgactcat gattaaataa gataccattg
     4081 catgcaaaca tgttgtatgc ctcacgataa ttcaccgtta tccagtaagc atacagcttg
     4141 gcaactccat acgggctgcg gggataaaat ggcgtagttt ctttttgcgg cacatcctgt
     4201 acctggccaa aaagctcgct ggtagaagcc tgataaaacc ttaccgtttt ttccataccc
     4261 agtatccgca aagcctctaa aatacgcaat gtccccagag catcagaatt tgctgtgtat
     4321 tccggcgttt caaagctaac ctgtacatgg ctctgagcgc caagattata aatttcatca
     4381 ggctgcacct gctgtataat ccgtattaca ttggttgaat ccgttaagtc gccatagtgc
     4441 aaaaaaaatg cagcaccgtc agtatgggga tcaacataca ggtgatcaat cctgtccgta
     4501 ttaaatgatg aagcacgtcg tttaatccca tgcaccacat accccttatt cagcaaaaat
     4561 tcagcaaggt atgctccatc ctgccccgtt atacccgtaa ttaacgcaac atcgctcatg
     4621 tacttatccc tttttttatt attgtaaggc acacaatacg tttcgtcatt ctaaaggcta
     4681 tcagtgccct gaaaatctat tcttttttat aaaagcgata tcctttgcct gcggcttaag
     4741 gatggcatgc acaccgttta gaataactat cgccacaaat acattctcag aacacaactg
     4801 tacaccttgc aacttacatc tcacacttca tacgcctaac tcttttcaac catccaccac
     4861 aaaccatcat ccatcaacca attatctgcc tgatgaatat tgcaacagcg ctataataag
     4921 ccctgcttgt gccagcactg cagttaaaaa
//
LOCUS       DAOOJH010000133         4940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13523_length_4940_cov_9.899284, whole genome shotgun sequence.
ACCESSION   DAOOJH010000133 DAOOJH010000000
VERSION     DAOOJH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13523_length_4940_cov_9.899284"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PLJ75_11630"
     CDS             complement(<1..1613)
                     /locus_tag="PLJ75_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05904.1"
                     /translation="MIDDELDRILQDIEPEQDYLTKKVNASKKYEEYILDEITEDNKE
                     ITYTPEKKEISLIARYFVNGLVNGIHRHIELSRSIRSRMDIAEIPEQIVPILKKSSYA
                     IFKLIEDIEILSDKCINEINETNLKRRATKDFPLYFADDFPDDNRLDFIVLKDTYVLL
                     RLFNSQIKKDWVELEKQTSVFNFSEGGKQLAFQLNDSIVEALQLCKATDILIELIANH
                     LGLPPHYYNAPDAEIKSRIQFAEFKSYTLPSIIGNIHKDENDTRSIESELQTLQADSS
                     DATINEKFEDEKLNPHNHKVESKKIFNRNDQNNELTKQANVFFTVKGSKPWNTRDPYI
                     LPVDENVLLVEEEDLEYMVYYSRTSMNEEQIKAELRRALLKLTIDRQKYDIVEEYIEY
                     INKKSISVLQEMLEYFHIDDTDQWLFYYHLGPQTICRIVLADMQLTGEGYCFSSRDGK
                     KIRKLLPYEFIKKMSLEWCENNINNRDIPFDSVNALDLIRRQVSKKYYSEVDEFVTKI
                     DSYLGTLDSEILKKLDRRLFIKQKALQIY"
     gene            complement(1631..>3166)
                     /gene="ftsH"
                     /locus_tag="PLJ75_11635"
     CDS             complement(1631..>3166)
                     /gene="ftsH"
                     /locus_tag="PLJ75_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPD05905.1"
                     /translation="QIQSTGNKAMSFGKVRAKLQPETKNKVTFADVAGVDEAKVELQE
                     IIEFLKDPKKFITIGAKIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEM
                     FVGVGASRVRDLFEQGKRNAPCIIFIDEIDAVGRLRGAGLGGGHDEREQTLNQLLVEM
                     DGFETNEGVIIVAATNRPDVLDPALLRPGRFDRQVVVDIPDIKGREKILAVHTRKIPL
                     AKDVDLKVIARGTPGFTGADLANLVNEAALLAARRNKKRVTMLEMEDAKDKVLMGPER
                     KSVLISAEEKKVIAYHEAGHAILGILTGTDPIHKVTVIPRGRALGLTMHLPKEERHIH
                     NKGYWLDQITILFGGHVAEKIKFGQVTTGASNDIERATDIARRMVCEWGMSETLGPLA
                     FGKKSEQIFLAREIAQHRDYSEQTAQLIDKEVHEIVTSCRDRAIILIEEHADKFELIA
                     TNLIERETLNAQEIELLMKGEPLPLIHNGDSEPPDEDEPKIGKAKRKTVSKSLLDKEI
                     IPT"
     assembly_gap    3167..3266
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3615..>4940)
                     /gene="tilS"
                     /locus_tag="PLJ75_11640"
     CDS             complement(3615..>4940)
                     /gene="tilS"
                     /locus_tag="PLJ75_11640"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPD05906.1"
                     /translation="SVSAGKDSMALLHILFVLRVRLDCTIAIYHLDHTVRGDESFDDL
                     LFVIQQAARYGIGAFVERYNFNQHKEKGKSFEQQAREYRYARLQMVAHEYGYTKIATA
                     HTCDDQIETLVMRIFQGTGLQGLQAIPLINNTIIRPLLSLHADDVYAYCTCNELSYRH
                     DLTNDDTHYLRNFIRHEVIPLVKKRFPTFDKALLNLQQQALGAVKSIEQLMAKVYPDC
                     IKQGGNHVEVPEDALSLNEYLFKFYCASIISNYLHRYCDTAMLDALYRAHVSDKKNVT
                     LFEGNGIFVYRRVKQSGAVLLFCSDPIMGDSNYEYTIPLGEDIYIDELGLTVRCMVME
                     STDAMPCTESKTIVLRYTGYGDIVVRNRRSGDTIFRKAGRRTLKRLYIDYKLTPEQKQ
                     KLPIIVVDNCVAAVLFDTIISQKPAISQTFLPLPHEKMLVIHYWYNHND"
BASE COUNT         1403 a         1040 c          795 g         1602 t
ORIGIN      
        1 ttatatatct gtaaagcttt ttgttttata aataatcgcc tatcaagttt tttcaaaatt
       61 tcagaatcca gcgtccccag ataagaatca atttttgtaa caaattcatc aacttctgaa
      121 taatattttt tagaaacctg ccttcgtatt aaatctaatg cattgacact atcaaacggt
      181 atatccctgt tattaatatt attttcgcac cattccagag acatcttctt aataaattca
      241 taaggtaata atttccttat ctttttacca tcacgtgaac taaaacagta accctcacca
      301 gtaagctgca tatctgctaa aacaatgcga catatagttt gagggcccaa atggtaataa
      361 aaaagccatt gatcggtatc gtcgatatga aaatattcaa gcatttcctg taataccgaa
      421 atactcttct tattgatgta ttctatatat tcttcaacaa tatcatattt ttgtctatca
      481 atagttaatt tcaataaagc ccttcgtaat tctgctttaa tttgctcttc attcattgat
      541 gttctggaat aataaaccat atattcaaga tcttcctctt caacaagcaa cacattctca
      601 tcaactggaa gaatgtaggg atcgcgcgta ttccatggtt tgctgccttt aactgtaaaa
      661 aaaacgttag cttgtttggt taattcatta ttttgatcat tgcgattaaa aatttttttt
      721 gattctactt tatgattatg gggattcagt ttttcgtctt caaacttttc atttattgta
      781 gcatcagatg aatctgcctg caatgtttgc agctctgatt caattgacct ggtatcattt
      841 tcatctttgt gaatattacc aattattgat ggcaatgtat aggatttaaa ttcagcaaat
      901 tgaattctgg atttaatctc agcatctggt gcattataat aatggggagg caatcctaaa
      961 tgattggcaa ttaactcaat taaaatatcg gttgccttgc ataactgtaa agcttcaaca
     1021 atactatcat tcaactgaaa tgctaattgc ttaccacctt cagaaaaatt aaatactgat
     1081 gtttgttttt ctaattcaac ccaatccttc tttatctggc tattaaataa tcgtagcaat
     1141 acataggtat ctttaagcac aataaaatcc agcctgttat catcaggaaa atcgtcagca
     1201 aaataaagcg ggaaatcctt tgttgctctc ctctttaaat tagtttcatt gatctcgtta
     1261 atacatttat ctgataaaat ctcaatatct tcaataagtt taaaaatagc ataggatgat
     1321 ttttttaaaa ttggaacaat ttgttcagga atttcagcta tatccatacg cgaacgaatt
     1381 gaccgagata attctatatg tcgatggata ccgttaacaa gaccatttac aaaatacctg
     1441 gcaattaatg atatttcttt tttttcagga gtataagtta tttctttgtt atcttcggtt
     1501 atttcatcaa gtatatattc ttcatatttt ttactggcat ttactttttt tgttaaataa
     1561 tcctgttcgg gttcaatgtc ctgcaatatc ctgtccaatt catcatcaat catgctaacc
     1621 tcaatgacat ctatgttgga attatttctt tatccaacaa tgatttggat acagtttttc
     1681 tttttgcctt tccaatcttt ggctcatctt catcaggtgg ttcactatca ccgttatgaa
     1741 ttagcggcaa cggttcacct ttcattaaca gttcaatttc ctgtgcattc agtgtttcgc
     1801 gttcaataag gtttgttgct atgagttcaa atttgtcagc atgttcttca attaatataa
     1861 tagctctatc acggcagctt gtaactattt catgcacttc tttatcaatg agctgtgctg
     1921 tttgctcgct gtagtctctg tgctgagcta tctcccgtgc aagaaagatt tgttcacttt
     1981 ttttaccaaa agcaaggggg cctaacgttt cactcatccc ccactcacaa accatcctcc
     2041 gtgcaatatc ggtggcacgc tctatatcat tgcttgctcc tgtggtaacc tgtccaaatt
     2101 ttatcttttc ggctacatgg ccgccaaaca atatggttat ctgatcaagc cagtatcctt
     2161 tattatggat atgtcgttcc tcttttggca aatgcattgt taatcccagc gccctgccgc
     2221 gtggtattac ggtaacttta tgaatagggt cagttcctgt caggataccc agaatagcat
     2281 gcccggcttc atggtatgca atgacttttt tctcctctgc tgaaataagc actgatttac
     2341 gttctggtcc catcaatacc ttgtccttag catcttccat ttcaagcatt gttacacgct
     2401 ttttatttct tcgcgcagcc agcagtgctg cttcattaac aagatttgct aaatcagcgc
     2461 cggtaaatcc cggtgtccca cgagcaataa ccttgaggtc tacatccttt gccaaaggaa
     2521 ttttgcgcgt atgaacagcc agtatctttt ccctgccttt gatatcaggt atatccacaa
     2581 caacctgacg atcaaatcgc cccggacgca gtaatgccgg gtcaagaaca tcaggacggt
     2641 tggttgctgc aacaataatc acaccttcat tggtttcgaa gccatccatt tcaaccagta
     2701 attgatttaa tgtttgttct cgttcatcat ggccaccacc caaccctgct ccacgcaatc
     2761 tgccaacagc atctatctca tcaataaaaa tgatacaggg agcattgcgt ttaccctgtt
     2821 caaaaagatc acgaactcgc gaagcgccaa caccaacaaa catctctaca aaatcagaac
     2881 cgctgatgga aaaaaatggt acgcctgctt ccccggcaac tgcacgtgcc agcaatgttt
     2941 tacccgttcc cggagggcca accagaagaa cgcctttggg aatttttgct ccaattgtga
     3001 tgaatttttt aggatctttt aaaaattcaa taatctcctg cagttcaact tttgcttcat
     3061 cgacacctgc aacatctgca aaggttacct tgttctttgt ttctggttgt aattttgctc
     3121 ttaccttgcc aaaactcata gctttgttac cggtagactg tatctgnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnttcg tatactttct ttatgaaatc actatattgt
     3301 agttgctcaa ccttctgaga atttacttca ttcatcctga atattgctat agccaataca
     3361 acgataagca gaagtagagc aacctgtttt gcagctttgt tcatcataat ctcccgtagt
     3421 aattatcact actgtttaag aagtcatagt atcaattgta gtattcactt acaagatact
     3481 acaattatgg aaatactcaa ataaattatt ccacccccta taacttttct gtatgatgtg
     3541 actatatact ttacagaaaa aaatatatat agggcatcag atgatgttac ttcatcgtaa
     3601 cgagtgagtg ttatttaatc attatgatta taccaatagt gaataacaag cattttttca
     3661 tgcggcaacg gcaaaaaagt ttgtgaaatt gctggttttt gggaaattat tgtatcaaac
     3721 aataccgcag ccacacagtt atcaacgaca atgattggta gcttttgctt ttgttcaggc
     3781 gtaagcttat aatcaatata taaacgcttc agggtgcgcc ttcctgcttt tcggaaaatc
     3841 gtatcaccac tgcgtctgtt tctgacaacg atatcaccat atccggtgta gcgcaacact
     3901 atcgttttac tttctgtgca tggcatggca tccgttgact ccataaccat acagcgtacg
     3961 gttaaaccca attcatcaat ataaatatct tcacccagcg gaattgtata ttcatagtta
     4021 ctatcgccca taatggggtc agagcaaaac agcaacactg cacccgattg ctttacccgc
     4081 cggtaaacaa atataccgtt accctcaaac agcgttacat tttttttatc cgatacatgt
     4141 gccctgtaaa gcgcatccag catagccgta tcacaatacc tgtggagata gttactgata
     4201 atggaggcac agtaaaattt gaataagtat tcattgaggg atagagcatc ctcaggcacc
     4261 tcaacatgat tgcctccctg tttgatacaa tcaggataaa cctttgccat aagctgctct
     4321 atacttttga cagcacccag agcctgctgc tgcaaattga gcaatgcttt atcaaaagtg
     4381 gggaaccttt ttttaaccag agggataacc tcgtgccgta taaagtttcg cagatagtgc
     4441 gtatcatcat tggttaaatc atgacggtac gatagctcat tacaggtaca atatgcatag
     4501 acatcgtctg catgcaatga caaaagcggg cggatgatag tattattgat aagcggtatt
     4561 gcctgtaatc cctgcagccc tgttccctga aatattcgca taaccaatgt ttcaatctga
     4621 tcatcacagg tgtgggcggt tgcaatcttt gtgtagccat attcatgtgc taccatttgt
     4681 aaacgggcat agcggtattc acgtgcctgc tgctcaaagc ttttgccttt ttctttatgc
     4741 tggttaaaat tataccgttc aacaaatgca ccaataccat accgtgctgc ctgctgtatc
     4801 acaaacagca ggtcatcaaa tgattcatca ccacgcactg tatggtcaag atgatagata
     4861 gcaattgtgc aatcaagcct tacgcgcaat acaaaaagaa tatgcagcaa tgccatggaa
     4921 tccttcccgg cagacaccga
//
LOCUS       DAOOJH010000134         4874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13751_length_4874_cov_10.398008, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000134 DAOOJH010000000
VERSION     DAOOJH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13751_length_4874_cov_10.398008"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(287..487)
                     /locus_tag="PLJ75_11645"
     CDS             complement(287..487)
                     /locus_tag="PLJ75_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05907.1"
                     /translation="MSSVKSDRNDGGYSVLGIYTFANTVKKCTVTMGFPKYKVLSAAG
                     KFITYCIENFKAFDKGKTMKNL"
     gene            604..2334
                     /locus_tag="PLJ75_11650"
     CDS             604..2334
                     /locus_tag="PLJ75_11650"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPD05908.1"
                     /translation="MKMSRYLVPTLKEDPSDAVITSHRLMLRAGLIRKESAGMYIYLP
                     LGLRVLQKIITIIREEMNKSGALEFLMPELTSADLWKESGRWHSMGPEMIRIKDRNGI
                     EYALAPTHEEAFTNVIRMVVASYKDLPVNAYQINTKFRDEIRPRFGVMRSKEFIMKDA
                     YSFDSDEAGLEDSYQIMRQTYRTIFKRCGLETIPVEADTGSMGGSNSEEFMVASEVGE
                     EVLLLCEVCGYKSNQERATCQYQLSPTNEDEEALAIVDTPDVRTINELVQFFGCSAHT
                     FLKSLIYVADGTPIMAVVPGDREINEVKLKNTLNAVDLELAPDAVVEEVTGAPVGFAG
                     PILVKGKALRIIFDTQIKTIKNAITGANQRDKHYKGVNPGRDFVIGQEADIVLVVAGD
                     RCPHCGNALIETRGIEVGHIFKLGYKYTRAMNVKVLDKNGKAITPIMGCYGIGVNRTM
                     AAVIEQHNDDRGIIWPMSVAPFQVHCISLAKNEKEEGAADTIYSMLVDAGIEVLYDDR
                     TASPGFKFGDADLIGIPIRITLGKAFFGDSSSIEVKLRNKDEKIIIAKDMIVDYCKKQ
                     IVQMLQKLNA"
     gene            2373..2531
                     /locus_tag="PLJ75_11655"
     CDS             2373..2531
                     /locus_tag="PLJ75_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPD05909.1"
                     /translation="MVEYITLTVLLVLSLAYSIRYIIRAIVTVNECNCDNCAIDCERK
                     RRPQKRLV"
     gene            complement(2562..2918)
                     /locus_tag="PLJ75_11660"
     CDS             complement(2562..2918)
                     /locus_tag="PLJ75_11660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD05910.1"
                     /translation="MVKPIDLEGRVVHDPILKKTKTGKSVCTFAIVLDYPTKGETKPY
                     PSYIDIETWDKLADLCAEHIKKGRLVQATGRLKQDRWQGEDGKARSKIKLVTKIVKFI
                     DPITSSKNNEEVVLQP"
     gene            3213..4631
                     /locus_tag="PLJ75_11665"
     CDS             3213..4631
                     /locus_tag="PLJ75_11665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HPD05911.1"
                     /translation="MATEKKSPNQELQEKLSVKKESAWLLLNDKVNDIYDFAEEYKQF
                     IGKNKTERLCVRSMVEMLKQKKYKDIQTVSKLLKGDAVYKNIKGKSLIACKIGSDPTK
                     IRIIGAHIDSPRLDLKPYPLYEDAEVALLKSHYYGGIKKYQWVNIPLAIHGVAFTKDG
                     HRVEIHIGENHDEPRFIIPDLLPHLAKDQMKKTGENIIEGEELNILFAHIPIDDKDIK
                     EKIKLNVLQYLHTTYGLVEEDFIAADIELVPAMQPVDIGIDKGLIAGYGQDDRACAYT
                     VWKALVDAGNCKDTVIMYLADREEIGSMGDTGAESFVFIHFMREYIRKTGITSTWDEL
                     LEGALSISADVTGALDPTFKNVNDTNNVSYLGHGVSIEKYGGRGGKYSTSEAHAEYMQ
                     YLRSLADKHKIPWQTGEQGKIDMGGGGTIAMFMSRYGMNCVDVGPCMLGMHSPYEVTS
                     KVDIYCAYRIYKAFFEDDTFKL"
     gene            complement(4640..>4874)
                     /locus_tag="PLJ75_11670"
     CDS             complement(4640..>4874)
                     /locus_tag="PLJ75_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05912.1"
                     /translation="LLWYIAFNPVRKRRCDSPVKYRYSSIQSYVQEGADVGVPIDHHE
                     YFLQLGDSFSERVRRFLQYEEAYRKRYSIGQWV"
BASE COUNT         1477 a          859 c         1074 g         1464 t
ORIGIN      
        1 cactctctat tttttttcca caaaaattct catttaccat tgaaacttta tgatagtacc
       61 ccctgttaaa agtattttga acagggccac ttcggtgttg ctccattctc cttcccggcc
      121 tttttgacca catactatga cgaacggtaa cctgcctgtt catagtgaca ttatgaacag
      181 attttgcttc tcaccgcaac tattttgact tttttatcat tcctccttcc ttaaagggaa
      241 cataaaaaca atggtaagcc ccgttacttg aatcttttag atttttttaa aggtttttca
      301 ttgtttttcc cttatcaaat gctttaaaat tttcaatgca atacgttatg aatttaccgg
      361 ctgctgataa aactttatac tttggaaatc ccatggtaac agtacacttt ttgacggtat
      421 ttgcaaatgt gtagatacca agaacagaat atccaccatc gttacgatca ctttttacac
      481 ttgacatgac atgtaccata tatcacaacc gttaccataa atacagtatg gggcactatc
      541 aattctatta ttatgtatgt cgttatgcat catgcaatat ccatttgatg agaggaaaac
      601 tatatgaaaa tgtcacgcta ccttgttcct acactgaagg aggatccttc tgatgctgtt
      661 attacaagcc acaggctcat gttgcgtgcc gggcttatac gaaaagaatc ggctggcatg
      721 tatatctatt tgccattggg tttgcgcgta ttacagaaga tcatcaccat tatcagggaa
      781 gagatgaaca aatcaggtgc tttagagttt ttaatgcccg agcttaccag tgccgattta
      841 tggaaggaat cagggcgctg gcattccatg ggtccggaga tgatacgcat aaaagaccgt
      901 aatggcattg agtatgcact ggcacccacc catgaagaag catttaccaa tgttattcgt
      961 atggtagttg cctcgtataa ggatttgcct gtcaatgcat accagatcaa tacaaaattt
     1021 cgtgatgaga tacgacctcg ttttggtgtt atgcgcagca aggagttcat catgaaggat
     1081 gcgtactcat ttgatagtga tgaagctgga ttggaagatt catatcagat tatgcggcaa
     1141 acctatcgta ccatctttaa acgatgcggt ctggaaacaa tcccggttga agccgatacc
     1201 ggttcaatgg gtggcagtaa ctcagaggaa tttatggtgg catcagaggt tggtgaggag
     1261 gtactgctgc tgtgtgaggt gtgtggctat aaatccaatc aggagcgggc aacctgtcag
     1321 tatcaactat cgcccacaaa cgaagatgaa gaagcgttag ctattgtcga tacccctgat
     1381 gtgcgcacca tcaatgagct tgtacagttt tttggttgta gcgctcatac atttttaaaa
     1441 agtttaatct atgttgcaga tggcacccct attatggcag tggtgccggg cgatagagag
     1501 ataaacgagg tgaaacttaa aaatacactg aatgctgttg acttagaact tgcacctgac
     1561 gctgtagttg aggaggtgac cggggcgcct gtaggttttg ccggtcccat tcttgtgaag
     1621 gggaaagcct taagaataat ttttgatact cagataaaga ctatcaaaaa tgccattacc
     1681 ggcgcaaacc agagggataa gcattacaaa ggtgttaatc caggacgtga ttttgttatt
     1741 ggacaggaag ctgatatagt actggttgtg gctggtgata ggtgtcccca ttgtggcaat
     1801 gcgcttattg aaacaagagg gattgaggtt ggccatattt tcaaattggg atataaatat
     1861 accagagcaa tgaatgtgaa agttcttgat aaaaacggta aggcaatcac gcctattatg
     1921 ggatgttatg ggataggtgt taaccgtact atggcagcag tcattgaaca gcacaatgac
     1981 gacagaggta ttatatggcc aatgagtgtt gctccgtttc aggtacattg catcagttta
     2041 gcaaagaatg aaaaagaaga gggtgcggct gacacaatct attcgatgct tgttgatgcg
     2101 ggcattgagg ttttgtatga tgatcgcacg gcaagccctg gctttaaatt tggcgatgcg
     2161 gatctaatag gcattcctat tcgcattacg ctgggaaaag cattttttgg cgatagttcc
     2221 tcgatagagg ttaagctgcg gaataaagac gaaaaaataa tcattgcaaa agatatgatt
     2281 gtggattact gtaaaaagca gattgtccag atgttacaga aactcaacgc ataaccttgt
     2341 ttcccggcgt tagtagtgct atacttgata ctatggtgga atatattaca ctaacggttt
     2401 tactggtgct atcgcttgct tatagcattc gttatattat cagggcgata gttactgtca
     2461 atgaatgtaa ctgtgacaat tgtgctattg attgtgaaag aaaaaggcgc ccccaaaagc
     2521 gccttgttta aaataatttt tagggaagag caaccacaga cttatggctg aagcacaacc
     2581 tcttcattat ttttgctgct tgttataggg tcaatgaatt ttactatttt agtgacaagc
     2641 ttaatcttgg agcgagcttt accatcctca ccctgccatc ggtcctgttt taaccgccct
     2701 gttgcctgga caagcctgcc ttttttgata tgttcagcac agaggtcggc aagcttgtcc
     2761 catgtttcaa tatcaatgta ggatggataa ggttttgttt cgccttttgt tggataatca
     2821 agcacaatgg caaatgtgca tactgatttt cctgtttttg ttttttttaa gattgggtca
     2881 tgaacaactc ttccctcaag atcgattggt ttaaccatag taacacctcg tttactataa
     2941 attttgataa agaacttttc attatataaa acgccggaga ttggaaaaat taacactttg
     3001 ggcaaaaatt ttttaatttt tttaaaaaaa ttttaagttt attaagctct gaccccaatt
     3061 ttctatgtga gtaaccttaa tgctggttta ttccctataa aaatgccatg aaaggattgt
     3121 gtttattaga tgcatgtgat atagtatagt gaaggaatta tgaaatacgt atctttgatt
     3181 aaataaatta cattaataag ggtgaagata gtatggcaac agagaagaaa tcaccaaatc
     3241 aggaattgca ggaaaagttg agtgtaaaaa aagagagtgc atggctatta ttgaacgata
     3301 aggttaatga tatatatgat tttgcggaag agtataaaca attcattggg aaaaataaaa
     3361 ctgagcgact gtgcgtgcgg tcaatggtag aaatgctgaa gcaaaaaaaa tataaggata
     3421 tccaaacggt tagcaaacta ctaaaaggtg atgccgttta taaaaacata aaagggaaaa
     3481 gtttaattgc ctgcaaaata ggctctgacc ccacaaaaat cagaattatt ggcgcgcata
     3541 ttgattcacc tagattagac ttaaaaccct acccattata tgaagatgcg gaggttgcac
     3601 ttttgaagtc acattattac ggagggataa aaaagtacca gtgggttaat ataccactgg
     3661 caatacacgg tgttgcattt acaaaagatg gtcatcgtgt tgaaatccat attggcgaaa
     3721 atcatgatga accgcgattc attattcctg atttactacc acaccttgca aaagatcaga
     3781 tgaaaaagac tggtgaaaat ataatagaag gtgaagaact taatattcta tttgcgcata
     3841 tcccgattga tgataaggac ataaaggaaa agattaaatt gaatgtattg caatacctcc
     3901 ataccactta tggactggta gaagaggatt ttattgctgc tgatattgaa cttgtacctg
     3961 ctatgcagcc tgtagatatt ggcattgata aagggcttat tgcagggtat ggtcaggatg
     4021 atagggcctg tgcttatact gtatggaaag ctttagtaga tgctggtaat tgcaaagata
     4081 cagttataat gtatttggca gatagggaag agataggttc tatgggtgat acaggagcag
     4141 aaagttttgt gtttatacat tttatgcgtg aatatatacg aaaaacgggt ataacgtcaa
     4201 catgggatga attgcttgaa ggagcgcttt cgatttcagc agatgttact ggtgcacttg
     4261 atcctacttt taaaaatgtc aatgatacaa ataatgtatc gtatttgggg catggggtta
     4321 gcattgaaaa atatggtggt aggggtggaa agtattccac cagtgaagca catgcagaat
     4381 atatgcagta tttgcgctca ttggctgata aacataaaat tccatggcaa acaggtgaac
     4441 agggtaagat cgacatgggt ggtggaggaa ccatagcaat gtttatgagt cgatatggca
     4501 tgaattgtgt tgatgtaggt ccatgtatgt tagggatgca ttcgccgtat gaggttacct
     4561 caaaggtgga tatttactgc gcctatcgta tctataaagc tttctttgaa gatgatactt
     4621 tcaagctctg accccttttt tacacccact gtccaatgga gtagcgcttg cggtatgctt
     4681 cctcatactg taaaaaacgt ctcactcgtt cactgaaact gtcccctaac tgcagaaagt
     4741 actcatgatg atctatcggt acaccaacat cagcaccctc ctgcacatag gattgtatgc
     4801 tgctgtagcg gtacttgaca ggactatcgc accggcgctt ccgtaccgga ttgaacgcta
     4861 tgtaccacag caac
//
LOCUS       DAOOJH010000135         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13834_length_4847_cov_7.639399, whole genome shotgun sequence.
ACCESSION   DAOOJH010000135 DAOOJH010000000
VERSION     DAOOJH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13834_length_4847_cov_7.639399"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLJ75_11675"
     CDS             <1..546
                     /locus_tag="PLJ75_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05913.1"
                     /translation="YKKTTLDDVAAQLTISRTALYYYYKNKDALFLALSQYELNKYEQ
                     ELKNAINACSTIEEMLVAFCKCFLPKRKQFREIYKLDPEESTLPFAIQKKIKSMNNAI
                     HINILTTIFKNDKKISQLSNLGYFMTILTYSIRGIVFSSFGVTAKQLENDMLSFFKIF
                     YNGLLYIISENSAHRNSKDKL"
     gene            543..1958
                     /locus_tag="PLJ75_11680"
     CDS             543..1958
                     /locus_tag="PLJ75_11680"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD05914.1"
                     /translation="MKIKLKSLPIILFTIIITTSTIVLAQDDTLNLNNAITISLQNNN
                     RYKIAQEKVHEKSFKINEVWGELWPQFSSDVSATRWVADKGTLTGSDGQYTIDIVKGT
                     IAINPGDFYNKLKASREDYIISVNDERKVKSDTIVKTIQLYYGVLLEQEMVKFRIDSL
                     RALEENLHVVETGYKNGMYTQLVFLRAKVAAANETTRLINARKDYEQTKSAFNIQLGQ
                     ETDMPVNLDTSPLHLTGDVDNNLFTVNDAELMNHYDEFVSIAVKNRPELLQLNHLKNF
                     YQYKEMQNDSVYLWPTFFVNGTYGATELINPKGDVDTGMGDTTNFIVNSFNSEINYKG
                     WNKNWNITIGAVYRWGTLSPVDATRSKSDEFKSLTQQTDLELEDFIKSLKLEIKDGLL
                     GLVAASHAIQSQRDNIASAKESVRVAVLQFKNGMIDNTELLNANVELSNATTMYIQAL
                     YDFQVSKAKLNRALGYDYFSF"
     gene            2017..2889
                     /locus_tag="PLJ75_11685"
     CDS             2017..2889
                     /locus_tag="PLJ75_11685"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HPD05915.1"
                     /translation="MSTKEELPVSSEKSSGKRKQSIPWWKTKASKITFKVLAALAVIT
                     LLIWFFIIRPYVRTDDARIAADIIRIANKGISGQIIAVNVEEGDRVSNGKVVVELDHR
                     TAEAQYQKAKARATFAKTELIRTEALARQNAVSKQQLDKVQAEAMSADADLKLAELAL
                     EYTTLTSPVNGIVVQKLARIGNILETNQTAITVVDIDNAWVDANIEETSVGDVKPGQK
                     VTIDIDEGGTLTGTVLEVRKATASTFALIPADNGSGNFIKLVQRIPIKIKLHPHPGKT
                     LRVGQSVTIKIRVK"
     gene            2899..4497
                     /locus_tag="PLJ75_11690"
     CDS             2899..4497
                     /locus_tag="PLJ75_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HPD05916.1"
                     /translation="MNTANQELNQNNYKWMVVAIVVIGTFMTALNGSIVNVSLPAIMA
                     DFGVNLDDIQWVVTGYMIAYATLMPLTAWLRDKIGYKILYIASLTVFTIGSIFCASAW
                     NLPALIGSRIIQALGGGAITPTGMAMITEAFEPHERGRALGLWGVGVILGPSIGPTLG
                     GYLTNHFGWRSIFMVNLPVSVIALIAASLFLIKEIPHKSQHRQFDFWGFAFLSIFLIT
                     LLLGLSKGEKEGWFSSYIITCAVMSALGFIGFILVESNTSYGIINLHLFKSPVFSICS
                     IIFLVRSVVLFGGIFLLPLFLQQQMGYDEMQTGLILLPSSIVMAFIMPFSGILGDKIG
                     ARLPTIFGLIGLWLFMYMYKNMDVTMSVYDIIFPTLLRGIGMGLLMAPVTAVAMNAVS
                     KKDAGMASSMLSILMQVGGSLGISILSTVLSHRIPFHVSSISSNIDSTSPAFTHAFHN
                     LFIHAHSLGFSYALSSQIAQGILFKHIAQHAVVLSFQDSFLVGSFMVAVAFILSFFLP
                     GRSARSRINPENEGEIIEEIAAID"
     gene            4661..>4847
                     /locus_tag="PLJ75_11695"
     CDS             4661..>4847
                     /locus_tag="PLJ75_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012481762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPD05917.1"
                     /translation="MTLEESYKLLQVSHNASDIEITKAFKKLALLYHPDKNPSNIDWA
                     NKAMASINVAYNTIITHR"
BASE COUNT         1609 a          881 c          923 g         1434 t
ORIGIN      
        1 tacaaaaaga ctacactgga tgatgttgca gcacaattaa ctatttcacg aactgctctg
       61 tactactatt ataaaaataa ggatgccctt tttttagcgt taagtcaata tgaattaaat
      121 aaatacgaac aagaattaaa gaatgccatt aatgcttgct caacaataga ggaaatgctt
      181 gttgcctttt gcaaatgttt tttgcctaaa cgaaaacagt ttagagaaat ctataagctt
      241 gatcccgaag aaagcaccct gccatttgcc attcaaaaaa agatcaaaag catgaataat
      301 gctattcaca tcaatatatt aacaactata tttaaaaacg acaaaaaaat tagccaatta
      361 agcaacctgg gatattttat gaccatttta acctattcta tacgaggaat agtattcagc
      421 tcatttggtg tcacagccaa acaacttgaa aatgacatgc tctctttttt taaaatattt
      481 tataacggtt tattatatat aatatcagaa aacagtgcac acaggaacag taaggataag
      541 ctatgaaaat taaactaaaa tcattgccga ttatactatt tacaataatt atcacaacca
      601 gtactattgt gctggcacag gatgatacat taaacttaaa caacgccatt actatctcac
      661 tgcaaaataa taatcgctat aaaatagcac aggagaaggt tcatgaaaaa tccttcaaaa
      721 taaatgaggt atggggagag ttatggcctc aattctccag cgatgtttcg gctacacgat
      781 gggttgcaga taagggaaca cttactggca gcgatggaca atatacaata gacatagtca
      841 aaggaactat cgccataaat cctggtgact tttacaataa gctaaaggca tcccgtgaag
      901 actacatcat cagcgttaat gatgagcgaa aagtgaaatc tgataccatt gtcaaaacaa
      961 tacagcttta ttatggagtg ctacttgagc aggaaatggt aaaattcagg attgattcgc
     1021 ttagggcttt agaagaaaac ttacatgttg tcgagacagg atataaaaat gggatgtata
     1081 ctcaacttgt ctttttacga gcaaaggttg ctgctgcaaa tgaaacaaca cgactcatca
     1141 atgccagaaa agattatgaa cagacaaaat cagcatttaa catacagctt gggcaggaaa
     1201 cagatatgcc tgtaaatctt gatacttctc cattacatct tactggcgat gtagataata
     1261 atcttttcac tgtaaatgat gccgagttga tgaatcacta tgatgagttt gtaagcattg
     1321 ctgtaaaaaa cagacctgaa ttattacagc taaatcacct caagaatttt tatcaataca
     1381 aagaaatgca aaatgactcg gtatacttgt ggccaacttt ttttgtaaac ggtacctatg
     1441 gagcaactga actaataaat cccaaggggg atgttgacac cggtatgggc gatacaacaa
     1501 actttattgt taatagcttt aacagtgaga tcaactataa agggtggaac aaaaactgga
     1561 atatcaccat tggagctgtg tataggtggg gaacattatc tcctgttgat gcaaccagat
     1621 ctaaatctga tgagtttaag agccttactc agcagactga tcttgagctt gaagatttta
     1681 taaaatcttt gaagcttgag attaaggatg gcttgcttgg gcttgtagcg gcatcacacg
     1741 ccattcaatc ccaaagggat aatattgcat ctgctaagga atcagtccgg gttgccgttt
     1801 tgcaatttaa aaatggtatg atagacaaca ctgaactttt aaatgcaaat gtagagttga
     1861 gcaatgcaac cacaatgtat attcaggctc tctatgattt tcaggtttcc aaagcaaaac
     1921 ttaatcgtgc acttggttat gattatttta gcttttaaaa atggaaatta aaatattgca
     1981 gctatgataa ttttataata gcaatgaggt ttaaggatgt ctactaaaga agaattacct
     2041 gtatcatcag aaaaatcgtc ggggaaacga aaacaatcta tcccatggtg gaaaacaaaa
     2101 gcatcaaaaa taacatttaa agtgcttgct gcattggcag ttattacact attaatctgg
     2161 ttttttatca tcaggcctta tgtgcgcacc gatgatgccc gcatagctgc cgacattatt
     2221 cgtatagcaa acaagggcat cagtggtcag ataattgctg tcaatgttga agaaggagac
     2281 agggtaagta atggtaaggt tgtggttgag cttgaccacc gtacagcaga agcacaatat
     2341 caaaaagcaa aagcaagagc tacttttgca aaaactgaac taataagaac agaagctctt
     2401 gccagacaga atgcagtaag taaacaacag cttgataaag tacaggctga agcaatgagc
     2461 gcagacgctg atttaaagtt agccgagctt gcccttgaat atactacact cacaagcccg
     2521 gtaaatggta tagtagtaca aaagcttgcc aggataggaa atattttaga aacaaatcaa
     2581 actgctatca ctgttgttga tattgataat gcatgggttg atgcaaacat tgaagagaca
     2641 tctgttggag atgtaaaacc gggacaaaaa gttaccattg atattgatga aggtggaaca
     2701 cttactggca cagtgctgga agtacgaaaa gcaactgcgt caacatttgc cctgatccct
     2761 gctgataatg gttcaggcaa tttcatcaaa ctggtacaac gcatacctat aaaaataaag
     2821 ctacatccgc accccggtaa aaccttacgg gttgggcaat cagtaactat taaaattcgt
     2881 gtaaagtagc aaaaacccat gaatactgct aatcaggaat taaatcaaaa taattataaa
     2941 tggatggtag ttgccattgt tgtcattgga acatttatga cagcactcaa tggcagcatc
     3001 gtcaatgtat cgttgccagc catcatggct gattttggag taaatcttga tgacatccag
     3061 tgggttgtca caggatatat gattgcctat gctactctca tgcctttaac cgcatggttg
     3121 cgcgataaaa ttggatacaa aattctttat atcgccagcc ttacagtttt tacaataggg
     3181 tcaatctttt gtgcatcagc atggaatctt ccagcattaa taggatcacg aattatacag
     3241 gcactgggag gtggagccat tacaccaaca ggtatggcaa tgattaccga agcatttgaa
     3301 ccccatgagc gaggaagagc attggggcta tggggtgtag gtgttatttt aggaccatca
     3361 ataggaccaa ctctcggagg ctatcttacc aaccattttg gttggcgttc aatatttatg
     3421 gtaaatcttc cggttagcgt tattgcgtta attgccgcat cattatttct tattaaagaa
     3481 attcctcaca aaagtcaaca caggcagttc gatttctggg gatttgcctt tttatcaata
     3541 ttccttatca cattactcct tggattatct aaaggcgaaa aggaaggatg gttttcttca
     3601 tacattatca cctgtgctgt tatgtctgct ctgggattta ttggcttcat tttagttgaa
     3661 tcaaacacca gctatggtat tatcaatctc cacctattca aatcacctgt tttttctatt
     3721 tgctcaatta tatttcttgt gcgctctgtt gttctttttg gggggatatt tcttttacca
     3781 ctgtttctgc aacaacaaat gggttatgat gagatgcaaa ccggccttat tttgcttcca
     3841 agctcaatcg ttatggcatt cataatgcca ttcagtggca tacttggcga taagattggc
     3901 gcacgattgc ccacaatttt tgggcttatt ggcttatggc tcttcatgta tatgtacaaa
     3961 aatatggatg taaccatgtc agtttatgac atcatttttc ctacattatt acgtggaatt
     4021 ggtatgggtc ttttaatggc acctgtcacg gcagtggcaa tgaatgctgt ttcaaagaag
     4081 gatgccggga tggcatcttc aatgctaagc atactcatgc aggtaggagg gtcattgggg
     4141 atatccatac tttcaacggt attaagccat agaataccct ttcacgtgtc atccatatct
     4201 tccaatatag atagcacatc accagcgttt acacatgcgt ttcacaacct ttttatccat
     4261 gcacattcac ttggcttttc ctatgcacta tcatcacaga tagcgcaggg gatccttttt
     4321 aagcatattg cacagcatgc tgttgtacta tcgtttcagg attcttttct tgtgggaagc
     4381 ttcatggtag cagttgcatt tattttatca tttttcctgc caggaagatc agcaaggagt
     4441 cgtataaatc ctgaaaacga aggtgagatt attgaagaga tcgcagcaat agattaaatc
     4501 cctgacacag taatagtaaa atcacaacgt tatgatattc gtaaacagta aaagcataat
     4561 ctttattttt ataaaaatgt aattgcattg ttatgcttat attgtataca cttatcagtt
     4621 tactaaaatt ggtacgctta ctaaccatca tataataatg atgactctgg aagaatcata
     4681 taaactttta caggtatcac acaatgcaag cgatatcgaa ataaccaaag cgtttaaaaa
     4741 acttgcattg ctataccatc ccgataaaaa tcccagcaat attgattggg caaataaagc
     4801 tatggcaagc attaatgttg catacaatac cataattaca catcgtt
//
LOCUS       DAOOJH010000136         4830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_13892_length_4830_cov_9.887749, whole genome shotgun sequence.
ACCESSION   DAOOJH010000136 DAOOJH010000000
VERSION     DAOOJH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13892_length_4830_cov_9.887749"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..777)
                     /locus_tag="PLJ75_11700"
     CDS             complement(1..777)
                     /locus_tag="PLJ75_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05918.1"
                     /translation="MATQNELFASTIFLKAALPLTKVILEYRPKMAAKYKGLNGVVQF
                     IAKDEAGNIGAFLQFSDGVLDVKYGIHEHPDIVFTFKSVKAMNDFFAGKPAVPSIKGL
                     TKLKLLIKSVNLLLALKIIGRKTPPKKPEDRELMVRLLLYLLPLGISQLNKAGYPDIA
                     KFCQKSPDRTFAWSVTGTDIASYLRMKAGNSKSGRGRYTRSKPFVEFKFDSIDGALGI
                     LLQTTDMMEASLNRVLVIEGSPEYGKEIGSFMNIVGQLAS"
     gene            complement(780..1793)
                     /locus_tag="PLJ75_11705"
     CDS             complement(780..1793)
                     /locus_tag="PLJ75_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPD05919.1"
                     /translation="MAADGLTYTIVDTANMSTAEIYGKVLCDLAEKHKEIVGLSADLA
                     KSTKMGQFAATFPDRFFNAGIAEQNMFGMAAGLAKAGLTPFVSTFAVFASLRAAEQVR
                     TDICYQKLNVKIIATHAGTSFGQAGTTHHCTEDLAVMRSFANMVVIAPADGIETANAV
                     MAAYEHKGPVYIRINRGFDPFLYEKTDYGFQIGKAVQLKDGTDITIIACGAAAYNAYH
                     AANILEKEDKLSVRVINMHTIKPIDEEAILKAVMETRRIVTFEDHNIIGGLGSAVAEV
                     IVKSGKGCAFETVGIPDQYAIIGLPEDLQNYYGFDQNKIIEKVRQIMGKDFEQTEDWE
                     DEV"
     gene            complement(1810..2652)
                     /locus_tag="PLJ75_11710"
     CDS             complement(1810..2652)
                     /locus_tag="PLJ75_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPD05920.1"
                     /translation="MPLTQQEIQQFTAKACQLRKDAIDTVYWAGGGHIGGSLSFTEIM
                     ILLYYKYARIDPKNPGWDDRDRIVVSKGHAGVGYAPILADKGYFPRELLKEFNHTGSI
                     LGMHLDGNKVPGVDVSTGSLGHGLPITVGLGIGARLQGKSWLTYCVLGDGECDEGSVW
                     EAAMAAAHYKVTNIITIVDRNRMMIDGPTEEVMRLEPFADKWKAFGWIVKEVDGHDFN
                     QLADAIEYAQKEDKAPVVIIAHTIKGKCVDFMEDKYEWHYGGLDSEKVELAKACIDKH
                     YCEL"
     gene            complement(2747..4048)
                     /locus_tag="PLJ75_11715"
     CDS             complement(2747..4048)
                     /locus_tag="PLJ75_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006501926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPD05921.1"
                     /translation="MKVEAKGIKYSVSSKSKSKTYTYTKSQKLFQRALKVIPCGIPGH
                     FSPAPFVPPTMYPFYAERSKGSHFWDIDGNEFIDYMCAYGPIVLGYNHPQVEKAAQEQ
                     NSKGNCMTAPGKIMVDLAEYLTDTIAMADWAFFAKNGGDVTNLAVMIARAATGRKKIV
                     MIKGGYHGVAPWMQGYGHYGVIEEDVANIITIDWNNVKQFEEVIAANPGAIAGFIATP
                     YHHPTFADSEFPADGYWQRIRELCTQHGIVLIIDDVRCGFRLDIRGSAEYFGFKPDLA
                     CYCKAIANGHPISALVGVDALKTVAAKVFYTGSYWYQAAPMAAALATLKELRRINAPV
                     LLNKIGKKLFEGMKKICASYGYELKITGAYSMPFVRITNDPSNMLHQEWCAECTARGA
                     YFTSHHNWFVCAAVSDKDIQKTWNIVDDVCKLLKKKYGDKY"
     gene            complement(4071..>4830)
                     /locus_tag="PLJ75_11720"
     CDS             complement(4071..>4830)
                     /locus_tag="PLJ75_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPD05922.1"
                     /translation="MDYEFIKSRLHLYAVLQNIEDLVQYDDVAKNLIKDWNIIVKFSV
                     RKAGSVYLIFENGKCTVRELSPKKPDVILFFTSPAHLNKMFDGKANPIPLKGITKLGF
                     LTKKFPKLTERLEYYLKPDEQKLKDKNYVTINTVMTLNTALYALKELLLFDPIGEHAA
                     KKVKGSMVMMNVNKGPAAYIEQKGNTFEVHKGYAQQPLAVLEFTSIKIANDFLNGKMD
                     AFSAIASGAVATKGHIEWLDAVSPVLDRVALYLS"
BASE COUNT         1414 a         1152 c          751 g         1513 t
ORIGIN      
        1 ttacgatgct aattgaccaa ctatattcat gaatgaacca atttctttac catattcagg
       61 tgaaccctca atgaccagta cacggtttaa tgaagcttcc atcatatccg ttgtttgcag
      121 taagattccc aatgcaccat caatactatc aaacttaaat tcaacaaatg gttttgagcg
      181 tgtgtatcta ccacgtcctg attttgaatt tccagccttc atgcgcaaat acgaagctat
      241 atcagtaccc gtcaccgacc atgcaaaagt tctatccggg cttttttgac aaaattttgc
      301 tatatccggg tatccagctt tattaagctg gctgataccc aacggtaaca gatataacaa
      361 taaccttacc ataagctctc tatcttccgg tttctttgga ggggtttttc ttccaataat
      421 ctttagcgct aacagtaaat ttactgactt tatcaaaagt tttaatttag ttaatccctt
      481 aatagatggc actgctggct tacctgcaaa gaagtcattc attgctttta cgcttttaaa
      541 ggtaaaaaca atgtcaggat gttcatgtat accatatttt acatccagta caccatcact
      601 aaattgcaaa aaagcaccaa tattacctgc ttcatcttta gctataaatt gaacaacacc
      661 attaagccct ttatactttg cagccatttt tggccggtat tccagaatca ctttggtaag
      721 tggtaatgca gcttttaaaa atatcgttga agcaaacaat tcattctggg ttgccataat
      781 cacacctcat cttcccagtc ttcagtttgt tcaaaatcct tacccattat ctgacgtact
      841 ttttcaataa tcttattctg atcaaacccg tagtagttct gcaaatcttc gggcagtcct
      901 attattgcat actgatcagg tatacccact gtttcaaagg cacatccctt tccgcttttc
      961 actattacct cagctactgc acttcccagt ccacctatta tattatggtc ttcaaaggta
     1021 actatacgcc gtgtttccat aacagctttt aaaatagcct cttcatcaat tggctttatg
     1081 gtatgcatat tgataacgcg tacacttaat ttatcctctt tttccagtat attggcagca
     1141 tgatacgcat tatacgctgc agcaccacac gcaataatag taatatcagt accatcttta
     1201 agctgtactg ctttgccaat ctggaagcca taatcagttt tttcatataa aaatggatca
     1261 aatcctctat tgatacgtat atacacaggg cccttatgct catacgccgc cattaccgca
     1321 ttagcagttt caattccatc tgcaggagct atcaccacca tgttggcaaa agaacgcatt
     1381 accgccagat cctctgtgca atgatgagta gtaccagcct gaccaaacga agttcccgcg
     1441 tgagtagcaa ttatcttgac attaagtttt tgatagcata tatcagttcg cacctgctct
     1501 gctgcccgca atgaagcaaa aaccgcaaac gttgaaacaa atggcgtaag ccctgctttt
     1561 gcaagccctg cagccatgcc aaacatattt tgttcggcaa taccagcatt aaaaaatctg
     1621 tcagggaagg ttgcagcaaa ctgtcccatc ttggtggatt tagccaggtc tgcagaaagc
     1681 cctacaatct ccttatgttt ttcagctaaa tcgcataaaa ccttaccata aatttctgcc
     1741 gttgacatat tggcagtgtc aacaatagta tatgttaatc catcagcagc cataataatc
     1801 ctccttacat tatagttcgc agtagtgttt gtctatacat gctttggcaa gctcaacctt
     1861 ttcactgtcc aacccgccat aatgccactc gtatttatct tccataaaat caacacactt
     1921 gcccttaatg gtatgagcaa taatgacaac aggagcctta tcctcttttt gtgcatattc
     1981 tatagcatca gccagttgat taaaatcatg cccatcaacc tctttcacta tccatccaaa
     2041 tgctttccat ttatcggcaa acggttcaag gcgcatcacc tcttcggtag gaccatcaat
     2101 catcattctg tttctatcaa caattgttat aatattcgtt accttataat gagcagcagc
     2161 catagcagct tcccataccg aaccttcatc acattcacca tcacccagca cacagtatgt
     2221 taaccatgat ttaccctgaa gtcgcgcacc aatccccaac ccaacagtga tgggcagtcc
     2281 atgacccagt gaacccgttg aaacatctac gccaggaacc ttgttgccat ccagatgcat
     2341 tcctaaaata gatccagtat gattaaattc ttttaacagc tctctgggaa agtaaccttt
     2401 atcagctaat attggcgcat acccaactcc agcatggcct ttacttacca ctattctgtc
     2461 gcggtcatcc catcccggat tctttggatc aattcgtgca tatttataat acaataagat
     2521 cataatttcg gtaaaactta acgaaccgcc aatatgtcca cctccagccc aatacactgt
     2581 atcaatagca tcttttcgta actgacaggc tttggctgta aattgttgta tttcctgctg
     2641 tgttaatggc atatatctcc tcctgtaaaa taatttcaca tgtatgtttt atgttcgata
     2701 gttccttata ctattctggt tgccttcaat acaaaagcaa gcataattaa tatttatcac
     2761 catatttctt ctttaagagt ttgcacacat cgtctacgat gttccatgtt ttctgtatat
     2821 ccttatcaga tactgccgca caaacaaacc aattatgatg agaggtaaaa tacgcacccc
     2881 ttgcagtaca ttcagcacac cactcctgat gtaacatatt actgggatca ttggtaatcc
     2941 ttacaaaggg cattgaatat gcacccgtta tttttaattc atatccatat gatgcgcaaa
     3001 tctttttcat accttcaaag agcttcttgc caattttatt taaaagaact ggagcattga
     3061 tgcgccgtaa ttcttttaat gtggcaagag ctgctgccat tggtgctgcc tgataccagt
     3121 aactgcccgt ataaaacacc tttgctgcaa cagtcttcaa tgcatcaaca ccaaccagtg
     3181 cagaaatagg atgcccattg gctattgctt tgcagtaaca tgcaaggtca ggtttaaaac
     3241 caaaatactc agcagagccc cgtatatcaa gtctaaatcc acatcgtaca tcatcaataa
     3301 ttaaaactat accatgctgt gtgcaaagct cacgtatcct ctgccagtat ccgtcagccg
     3361 gaaattcact gtcggcaaag gttggatgat ggtaaggagt tgctataaaa ccagctatcg
     3421 ccccgggatt tgctgctatt acttcttcaa attgttttac attattccaa tcaatggtaa
     3481 taatatttgc aacatcttct tctataacac cataatgacc atacccctgc atccatggag
     3541 caacaccatg gtagccaccc tttatcataa caattttttt tcttcctgta gctgcccgag
     3601 caatcatcac tgctaaattg gtaacatcac caccattctt tgcaaagaaa gcccaatcag
     3661 ccatcgcaat ggtgtcagta agatattcag ctaaatctac cataatttta cccggagctg
     3721 tcatgcaatt acctttacta ttttgctcct gagcagcttt ctctacctga ggatgattgt
     3781 accctaaaac aatgggtcca tacgcacaca tataatctat aaattcattc ccatcaatat
     3841 cccagaaatg tgaacctttg gacctttcag cataaaaagg atacatcgtt ggcggcacaa
     3901 acggcgcagg actgaaatgt cccggaatcc cgcacggtat aaccttaagt gcgcgctgaa
     3961 atagcttctg agactttgtg tacgtatatg ttttgctttt tgattttgat gaaacgctat
     4021 acttaattcc ctttgcttct actttcatac tattacctca cggttacagg ttatgataaa
     4081 tacaaagcta ccctatcaag caccggactg actgcatcaa gccactcaat atgacctttt
     4141 gtagccacag cacctgaagc aatggcactg aatgcatcca tctttccatt caaaaaatca
     4201 ttggcaattt ttattgaagt aaactccagt acagctaatg gttgctgtgc atatccctta
     4261 tgcacctcaa aggtattgcc cttttgctca atataggcag caggcccttt gttgacattc
     4321 atcatcacca tgctaccctt cacttttttt gctgcatgtt cgccaatagg gtcaaacaat
     4381 aacaattctt tgagcgcata caatgcggta ttcaaagtca ttaccgtatt tatagtaaca
     4441 taatttttat ctttaagttt ttgttcatca ggttttaagt aatattcaag ccgctcggta
     4501 agtttgggaa attttttggt taaaaaacca agttttgtaa ttccctttaa cggaattgga
     4561 tttgcttttc catcaaacat cttgtttaaa tgagcaggtg aagtaaaaaa caagatgacg
     4621 tcaggttttt ttggcgatag ttccctgaca gtacattttc cgttttcaaa aatgagatac
     4681 actgaacctg ctttccttac actaaacttt acgatgatat tccagtcctt aatgagattc
     4741 tttgcaacat catcatactg caccaaatcc tcgatatttt gcaacactgc atacagatgc
     4801 aatcgtgatt ttataaattc ataatccata
//
LOCUS       DAOOJH010000137         4794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14010_length_4794_cov_8.934374, whole genome shotgun sequence.
ACCESSION   DAOOJH010000137 DAOOJH010000000
VERSION     DAOOJH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_4794_cov_8.934374"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1533
                     /locus_tag="PLJ75_11725"
     CDS             43..1533
                     /locus_tag="PLJ75_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HPD05923.1"
                     /translation="MKQRFKKHFERKPIEELMEEAGHEHEYSLKRVLSPFHLIMLGVG
                     VIVGAGIFVLTGHVAAAYSGPAIVLSFVIAAIACGFAGLCYAEFASMISVSGSAYTYA
                     YATLGKLFAWIIGWDLILEYLFGASSVAVGWSGYVCSFLKDFGIVIPASFASAPFGYD
                     IHTGWYLTGAIVNVPAVLIIVIMTILLILGIKESATFNNIIVVIKVSVIVLFIGFGIF
                     FINFENMVPFIPPNTGHFGEYGFSGILRGAGVIFFAYIGFDVVSTAAQEVKNPKRDMP
                     IGILGSLAIVTVLYILVAFVITGLVSYTKLNVPDPIAVAIDAAGEHLFWLRPIIKIGA
                     ITGLSSVVLLLLYGQSRIFFTMARDGMLPSVFARVHQKYRTPYITTIVVGVAAVIFSG
                     LFPIGILGEMVSIGTLLAFVIVCIGVLVLRYTRPKLHRPFKTPFVPFVPIMGALVSFI
                     QMVALPKDTWLRLLIWMFVGFIIYAIYGESHAHKKPTEDKEALLIK"
     gene            1614..1760
                     /locus_tag="PLJ75_11730"
     CDS             1614..1760
                     /locus_tag="PLJ75_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05924.1"
                     /translation="MRQSAIKAKELSLNEEVIRIEKLKVLEEIEKAIFEAVATQFTAI
                     STRF"
     gene            1905..2594
                     /locus_tag="PLJ75_11735"
     CDS             1905..2594
                     /locus_tag="PLJ75_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05925.1"
                     /translation="MVKRLLLLVTGFLFVHSVLYAYRPFATEDAGVAPLGENKLELGS
                     EVSGFSRDTLNNANLSFLMGIGLGKAEILFETPYCITNEEGEENEGLEDFVLAAKVKV
                     AGSNQDTGFTLKTEYAHESGMYGLSGVVSQSFSWFTLHSQCGLISDFDDDGAIFGLGI
                     DFPITDNFSLIIDSFVEHIADDTRYQILCGGIVAIQDTIVLDAALGYSWIDRWSNERE
                     EIAIIGCSIVL"
     gene            2788..2940
                     /locus_tag="PLJ75_11740"
     CDS             2788..2940
                     /locus_tag="PLJ75_11740"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPD05926.1"
                     /translation="MNYNDTISSMSIGERAIIAGFQNKDRKYREQLLSMGLVKDTEFI
                     SVLNVP"
     gene            complement(3011..3352)
                     /locus_tag="PLJ75_11745"
     CDS             complement(3011..3352)
                     /locus_tag="PLJ75_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05927.1"
                     /translation="MELQQRLSLTAGILLIISGCTHITQLTVYQHHGHVIAASLFGVV
                     YLLPGVFIIILMKKTWAIIPGAIVPFIGGTPGVMRFFFVHANPFSVFHVLLDLMIVPF
                     CIASIILSRKE"
     gene            3582..4424
                     /locus_tag="PLJ75_11750"
     CDS             3582..4424
                     /locus_tag="PLJ75_11750"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPD05928.1"
                     /translation="MADSICGRSSWLQVYNLIIRILLMKLMNRKMVITAVVSCMVIAV
                     STFTIYNNNRKTVKPRVTSVVDAVYALGIVTPYKEYSLRVGMSTRIEKLYIWEGDRVQ
                     AGSPLVQLDGGILLRAPFSGVVATLNRKESEIVSAQESIMTIVDPATMYIRLSLDQES
                     IINVRKKQVAEISFENLRNKNIRGVVSRIYPSEGEFVVAIDVDSFPEGVLPYMTCDVA
                     IKVSEKNNALLLPKNAVHNGAVTVIRNGRKQLVNVSTRAHDSGWVEIVQGNITPDDTV
                     VVMR"
     gene            4467..>4794
                     /locus_tag="PLJ75_11755"
     CDS             4467..>4794
                     /locus_tag="PLJ75_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD05929.1"
                     /translation="MKYIISRKVQSLLMLIGVTLGCGGYIIFTSVQLGFQTFMKERLI
                     ENNGHITIESREEDITRESMEGVFFKGSTVYWISGPSGRRSNEYLQSAWHWQKRLADI
                     PFVVAST"
BASE COUNT         1445 a          744 c         1076 g         1529 t
ORIGIN      
        1 taatcaatga acattaatat ttactgatgc aggtgctatg gcatgaagca gcgttttaaa
       61 aagcattttg aacgtaagcc aatagaagaa cttatggaag aggccggtca tgaacatgag
      121 tattcactga agcgagtact cagtccattt catttaatta tgcttggtgt aggtgtaata
      181 gttggtgccg gtatatttgt acttaccggt catgttgctg ctgcatactc gggaccggca
      241 atcgtacttt catttgtcat tgctgcaata gcatgcggtt ttgcagggct ctgttatgct
      301 gaatttgcat ccatgatatc agtttcagga agtgcatata cctatgcata tgccacgtta
      361 gggaagctct ttgcatggat cattggctgg gatcttattc ttgaatatct ttttggagct
      421 tcgtcggtag ctgttgggtg gtcagggtac gtgtgtagct tcttgaaaga ttttggtatt
      481 gttattcctg cttcttttgc cagtgctcca tttggatatg atattcatac gggatggtac
      541 ctaacaggtg caatagtaaa tgtgcctgct gttctgataa tcgttatcat gaccattttg
      601 ttgatactgg gaataaagga atctgctacc tttaataata tcatcgtagt tattaaggtt
      661 agtgtcatag tgcttttcat cggatttggt atatttttta taaattttga aaatatggtt
      721 ccctttatac cgccaaacac cggacatttt ggcgaatatg gatttagcgg tatattacga
      781 ggagcagggg taatattttt tgcatacatt gggtttgatg ttgtatcaac ggcagcacag
      841 gaggttaaaa atcccaaacg cgatatgccc attggcatac ttggttcatt ggcgatagtt
      901 actgttttat acatcctggt tgcctttgtt attaccgggc tggtaagcta tacaaaatta
      961 aacgttccag atcctatagc tgttgctatt gatgctgctg gcgagcatct attctggttg
     1021 cggcctatta taaaaattgg agcgataact ggtttgagtt ctgttgtgtt gctgttgctc
     1081 tatggacagt caagaatatt ttttactatg gcacgggatg ggatgctgcc atctgttttt
     1141 gccagagttc accaaaagta caggacaccg tatataacaa ccattgtagt aggtgttgcc
     1201 gctgtcattt tttctggatt gttccccata ggcatccttg gtgaaatggt atcaattgga
     1261 acattacttg catttgttat tgtgtgtatt ggagtgctgg tattgcggta tactcgtcct
     1321 aaactccaca ggccctttaa aacgcctttt gttccgtttg ttcccatcat gggagcgctg
     1381 gtatcattta tacagatggt agcattgcca aaagatacat ggttgagatt gctcatctgg
     1441 atgtttgtag gattcattat atatgctatc tacggcgaga gccatgccca taaaaagcct
     1501 actgaagata aagaagcatt attaataaaa tgattattga taagatagtg taacattcct
     1561 attaaatatt cttattcaac ccaataaaag taaaaaccaa cattgaggat tttttgcgtc
     1621 aaagcgcaat caaagcaaaa gaattatcac tcaatgagga agttatcagg atagaaaaat
     1681 taaaagtgtt ggaagaaata gaaaaagcta tctttgaggc agtggcaaca cagtttacag
     1741 caataagcac tcgtttttaa gagattgata aacgttttac tactcaacag tggattatag
     1801 attgagtaaa gaattatttt ttattgataa atatgagtta tattagatat gtatgatata
     1861 attagtcata actaattata ttaattctat atcaggattc tttaatggta aaacggttat
     1921 tgttgcttgt cacaggtttt ttatttgttc atagtgtact ttatgcatac aggccatttg
     1981 ctacggagga tgcaggtgtt gcaccgctgg gtgaaaataa gctggaattg gggtctgaag
     2041 tatctggttt ttcacgggat acgcttaata atgcaaatct ttcgttttta atgggcattg
     2101 ggcttgggaa ggctgagata ttgtttgaaa ctccttattg cataacaaac gaagaaggtg
     2161 aagaaaatga aggattagag gattttgttt tagcggcaaa agtaaaggtt gcaggcagca
     2221 accaggatac agggtttaca ctgaaaacgg aatatgcgca tgaatcggga atgtatgggt
     2281 taagcggtgt ggtgtcgcaa tcgtttagct ggtttacttt gcattcacaa tgtggattga
     2341 ttagtgactt tgatgatgat ggtgcaattt ttggattggg gattgatttt cccataaccg
     2401 acaactttag tttgataatt gattcttttg tagagcacat tgctgatgat acgcgttatc
     2461 agatcctttg cggaggtatt gtagctatac aggataccat tgtgcttgat gcagcattag
     2521 gttattcatg gatagatagg tggagcaatg aaagagagga gattgcaatc attggctgct
     2581 ctatagtgtt gtaaatgatt tttgttaaaa tatttgtatt aactaataat agtatatact
     2641 aatattttta cattcattat ttttgtttcc actcgcatgg atacatgctg aagaatggtt
     2701 aaatattaaa agtatggaac tcattatgta tacgtttgca aataatacta ttgataagat
     2761 tctatcaaat taaagctggg agtatacatg aattataacg acacaatctc atcaatgtca
     2821 ataggtgaga gagcaataat agcgggcttt caaaacaaag atagaaaata cagggaacaa
     2881 ctactctcca tgggacttgt aaaagataca gaatttataa gcgttctgaa tgttccttaa
     2941 caggtaactg tttgttatac aatactgcta cggctacgat gttctatgta tggtggatta
     3001 acattatcta ttattccttg cgtgataaaa taattgaagc aatacaaaag ggaactatca
     3061 tcagatcaag aagtacatgg aaaacactga agggatttgc atgaacaaaa aagaatcgca
     3121 tcactcctgg tgtcccacca atgaaaggaa ctatcgcccc aggtataatt gcccatgtct
     3181 ttttcatcaa gattataata aacacaccag gtaaaagata tactacgcca aataatgatg
     3241 cagcaatgac atgaccatga tgctggtaca cggtaagctg ggtaatgtgt gtgcatcctg
     3301 atataataag gagtatgcct gctgttaagg atagtcgttg ttgcagttcc atagcgcacc
     3361 tcaaaatatt ttgatggtat tgatataatt atatactacg tgttcaaaac aacaattgca
     3421 agaataaatg gctgcacatt tttttataaa aatatcttgc catgcatata cttttgagga
     3481 tgtgattatg agttgaaaat aaaaccggat agtatgctgc atttgatatt aaaaaattgg
     3541 aactttatta tgacaaccat ggtctaaggt tgattgtagt aatggcagac agtatatgtg
     3601 gcaggtcatc atggttacag gtgtataatt taattatcag gattttatta atgaaattaa
     3661 tgaatagaaa gatggttatt acagcagtgg tttcatgtat ggtgattgca gtaagtactt
     3721 ttaccattta caacaataat agaaaaaccg ttaaaccaag ggtaacctca gttgttgatg
     3781 cggtatatgc gttagggatt gttaccccct ataaagaata tagcctgcgt gttggcatga
     3841 gtaccaggat tgaaaagctc tatatatggg aaggtgatag ggtacaggca ggaagccctt
     3901 tagtgcaact tgatggaggc atactattgc gtgctccttt ttccggggtt gtggctacct
     3961 taaaccgcaa ggaaagtgag atagttagtg ctcaggaatc catcatgaca atagttgatc
     4021 ctgcaacaat gtatatcagg ctttcacttg accaggaatc gatcattaat gttagaaaaa
     4081 aacaggttgc tgagattagc tttgaaaacc tgcgcaataa aaatatacgt ggcgtggtaa
     4141 gcaggatata tccatcagaa ggggagtttg ttgttgctat tgatgttgat tcatttcctg
     4201 agggcgtatt gccttacatg acgtgcgatg tggcaattaa ggttagtgaa aaaaacaatg
     4261 cgctattact gccaaagaat gctgtccaca atggtgctgt aacagtaatt cgtaacggca
     4321 gaaaacaatt ggtgaacgtc agcacacgtg cacacgacag tggatgggtg gagattgttc
     4381 agggcaatat aacccctgat gatactgtgg ttgttatgcg atgagctatt gcctaaaaaa
     4441 ggaatgcgga aatgtttgag ttagcaatga aatatattat ctcgcgtaag gttcaatcgt
     4501 tgcttatgct aattggtgtt acactgggat gtggcggcta tattatcttt acctcggttc
     4561 aacttggttt ccagacattt atgaaggagc gccttataga aaataacggg catattacca
     4621 ttgaaagcag ggaagaagat attacccgtg agtcaatgga aggtgttttt tttaaaggga
     4681 gcactgttta ctggataagc ggtccaagcg gcaggcgctc caatgagtat ttgcaaagcg
     4741 catggcactg gcaaaaacgc ctggcagata ttccatttgt tgtagcaagt acaa
//
LOCUS       DAOOJH010000138         4793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14014_length_4793_cov_7.085057, whole genome shotgun sequence.
ACCESSION   DAOOJH010000138 DAOOJH010000000
VERSION     DAOOJH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14014_length_4793_cov_7.085057"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLJ75_11760"
     CDS             <1..515
                     /locus_tag="PLJ75_11760"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPD05930.1"
                     /translation="VLLPEVPFELYGANGLCAHLENRLQRRGHAVICVAEGAGQEILQ
                     KDSDKQYDASGNVKLADIGVYLKEKIQEYFKQKEIEINLKYIDPSYMIRSAPAVPNDS
                     IYCTLLGQNAVHAGMAGKTDLVIGQWSNIYTHVPVEVAISKRKRIDPHSRFYSSVLDA
                     TGQPFAMINK"
     gene            542..1060
                     /locus_tag="PLJ75_11765"
     CDS             542..1060
                     /locus_tag="PLJ75_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05931.1"
                     /translation="MPKEHKNAPLICAVITGIAIAGAGIGIGKSMPLIIALGIIPAIV
                     YEIYRTQGMFTTLASWLALIVTIAALYVIYKNTMIDIAPYISKCMTLPVNTKLIPVAY
                     VAPVVLVVIAVFLFKQTAGIYTRWLALVILLTSVALFYSLDPSFIKNLLSTPQVQQNI
                     KEGVKRGIHTIH"
     gene            complement(1066..2220)
                     /locus_tag="PLJ75_11770"
     CDS             complement(1066..2220)
                     /locus_tag="PLJ75_11770"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPD05932.1"
                     /translation="MQPSNGIIYEFFIFCFKVASLLMRHKAHLKGKEYLKKIPHLVIF
                     APTHDSYFEVPSLSKVYYSLKPRPHFVVMAKKDFLSGNYLASNFGEKNKLLYYFFYFL
                     DKTGLPKAFFKKMKLIDIPRPFIEDFDNKNQVKHEIHSQMVKFKHSIHSGFSTLIFPE
                     GTTWSFGGLKNLRSGIFQLVDSTYRHFRQKLYILPINVKVDRCIAGKKDIFINVGEPF
                     FMLGDKEKFLDRVSEILKSLHTITFSQIAATYLQRMAMAVEHIDKAIVLKKEELASQL
                     DTIINDINVLVKEGKLPSIDEYLLDSNYRHKKLKRFLKFCIKKGYIEKIDGYYMINVQ
                     RVLASFHIKSYRKENPVGFTANEFLSINYNCIMTIYEKYCNTYKVSTPIR"
     gene            2616..3362
                     /locus_tag="PLJ75_11775"
     CDS             2616..3362
                     /locus_tag="PLJ75_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD05933.1"
                     /translation="MVIDHMEEFKYSKESRTIILQAAKMEFAEKGYDGARLSSIAKRA
                     GVNQALIHYYFSNKEKLYHEVLQYLFHHEEIGIIREHLKDVTLSPQEALFTAIYILVY
                     LHHNAIDPEINKIISWELAQGGDKFKMLLTDYMLPKLNMLSQVIQEGIESGDFETDDP
                     FLVVFNLTNMVLSFENSKQYLKDTPWYDRFYGGDDGKKLFEFALKSTFKSLAPKHKAC
                     EIPQINEDLKHKLEEIMQKLKNDSEHENDS"
     gene            3352..>4793
                     /locus_tag="PLJ75_11780"
     CDS             3352..>4793
                     /locus_tag="PLJ75_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPD05934.1"
                     /translation="MTVKYNTIIKTMLPVFMYSILFMITATGTIYAQNPLTLKEAIDI
                     AIKNNNTYKIALLKVEENRYKVRESWGMLWPKLSTDVSYTRQWYEAGFQSQIEGQYNV
                     KIINGQISINPGTFYNTLQSSRNNFIASEHDARRVKADTTITTLRLYYQILLTKELIT
                     MHEESLKALEENLRIVTVGYQKGTFSKLDFLRARVAYNNEKTQLINAKNDYLTVCATF
                     NIHLNREINEAVILDESVLKVHEEDLQYLALEEAKKNEIVGQLVALALKNRPELLQLS
                     YSKTALEYATSATSSIYLWPSFFVNGSYGSSKNISKDGGGGLTPTGDPATDQILQALS
                     QGMSQSFAPSGWNDNWMITFGATYQWGGWSPLDSTHAKAKQLESQTNQVDLQLEDFVK
                     SIRLDIQRGFLKLESASNSIYSQQGNVETAEESLKTATTQFRNGIIDNSKFLEANVAL
                     VQAKTLYIQSLYDYKVSQAELNKATGIDYFT"
BASE COUNT         1600 a          843 c          884 g         1466 t
ORIGIN      
        1 ttgtattatt gcccgaggtt ccctttgaac tgtatggagc aaatggatta tgcgcgcatc
       61 ttgaaaatcg gttacaaaga agaggacatg ctgtcatatg tgttgctgaa ggtgcaggac
      121 aggaaatatt gcaaaaagat tctgataagc aatatgatgc ttcgggtaat gttaaattag
      181 ctgacattgg tgtctatcta aaagaaaaga tacaggaata ttttaaacaa aaagagattg
      241 aaattaattt aaaatacatt gatccaagct acatgatacg gagtgctccc gcagttccca
      301 atgattctat ctactgcaca ttattggggc agaatgctgt tcatgcaggt atggccggaa
      361 aaacagattt agtcattggc caatggagta atatatacac ccatgtcccg gttgaggttg
      421 caatttcaaa acgaaaacgc atagaccccc attcgcgctt ctatagcagc gtacttgatg
      481 caacaggaca accttttgcg atgataaata aataataatt gtattgtaaa ggaggtttgc
      541 aatgccaaaa gaacataaaa atgcgccact tatttgtgca gttattaccg gtatcgcaat
      601 agcaggagct ggtataggca tagggaaaag catgccactt attatagctc tcggaattat
      661 tccagctatt gtttatgaaa tatacagaac acagggtatg tttacaacat tggcatcatg
      721 gttagcgctg atcgttacaa tagcagcgct ctatgttatc tataaaaata ccatgattga
      781 tattgccccc tatatatcaa aatgtatgac actgccggtt aacacaaagc ttatccctgt
      841 tgcatatgtt gcaccggttg ttcttgtcgt tattgctgta tttttgttta agcaaacagc
      901 gggcatctat acccgctggc tggcgcttgt gatactgtta acatcggttg cgcttttcta
      961 tagtctggac ccttcattta ttaaaaatct actttcaact ccgcaggttc agcaaaacat
     1021 caaagagggc gtaaaacggg ggatacacac tattcattaa acccgttacc gtataggcgt
     1081 acttactttg taagtattac agtatttttc atatattgtc ataatacaat tataattaat
     1141 ggataaaaat tcgttagcgg taaatccaac aggattttct tttcgatagc tcttgatatg
     1201 aaatgaagcc agtacacgtt gaacattgat catataatac ccatcaatct tttcaatata
     1261 tcctttctta atgcaaaatt ttaaaaaccg ttttaatttt ttgtggcgat agttactatc
     1321 aagaagatat tcatcaatgc tgggcagttt cccttctttg accagcacat taatgtcatt
     1381 gatgatagta tctaactgtg acgctaattc ttctttttta agaactatcg ctttgtcaat
     1441 atgttcaacc gccattgcca tacgctggag ataggttgca gcaatctgac tgaaggttat
     1501 ggtatgtaaa ctttttaaaa tttcagaaac tctatctaaa aatttttctt tatccccaag
     1561 cataaaaaag ggctccccaa catttataaa aatatccttt ttaccggcta tgcaacggtc
     1621 aaccttaaca ttaattggca gtatatataa tttttgcctg aagtggcgat aggtactatc
     1681 aacaagctgg aatatcccac tgcgtaaatt ttttaaacct ccaaaactcc acgttgtccc
     1741 ttccggaaat ataagcgtag aaaaaccact atgtatagaa tgtttaaact ttaccatctg
     1801 agagtgtatc tcatgtttca cctgattttt attgtcaaaa tcttctatga aggggcgtgg
     1861 aatatcaatc agtttcatct ttttgaaaaa agcttttggc aaccctgttt tatctaaaaa
     1921 ataaaaaaaa taatagagca gtttattctt ttctccaaaa tttgatgcca gatagttccc
     1981 acttaaaaaa tctttttttg ccattaccac aaagtgtgga cgtggtttca atgaataata
     2041 tacctttgaa agggatggta cctcaaaata gctatcatgt gtgggggcaa atattacaag
     2101 gtgaggtatt ttttttagat attctttacc ttttaaatga gctttatgcc gcataagcag
     2161 tgaagcaact ttaaaacaga aaataaaaaa ttcatagata atgccatttg atggctgcat
     2221 atatataccc cataccactt tgctggtata aaaataaatc attcaccagc aattgtcaat
     2281 agtaaaaata cataaaagta taatgaaaag catggcgttt tcattttcaa ccgattgcat
     2341 tcctcttgaa atgggatact gtgtcattct gagggacgca gtcccgaaga atttatcctt
     2401 tcatgaaaag gcaaaattct tcacttcgct cagaatgacc tatactatag gtattatcag
     2461 atgatatatc atacaatatt tttttatatt tttttatatt tttttatttt ttttattttt
     2521 ttattttgct attgacaaaa attggtatat atttaactaa tcaatttaac tttttagtta
     2581 aaataactga ttgccatgga taacgactta aaactatggt tattgatcac atggaagaat
     2641 ttaaatattc aaaagaaagt cgaactatca tcttgcaagc tgcaaagatg gagtttgccg
     2701 aaaaaggcta tgatggcgcc cgtttaagct caattgcaaa acgtgcggga gtaaaccagg
     2761 cgctcatcca ctattatttt tcaaataaag aaaaactata ccatgaagtg ctgcaatatc
     2821 tgtttcacca tgaagaaatc ggcattattc gggaacattt aaaagatgtt acattaagcc
     2881 ctcaagaggc tctttttaca gcaatctaca tactggtata tctgcatcat aatgccattg
     2941 atcctgaaat aaataaaatt atttcatggg aattagcgca gggtggggat aaatttaaaa
     3001 tgctcctcac cgattatatg ttacccaaac tcaacatgct gtcgcaggta attcaggagg
     3061 gtatagaaag cggggacttt gaaactgatg atcctttcct tgttgtcttt aaccttacca
     3121 atatggtttt atcatttgaa aatagcaaac aatacctcaa agacacgcca tggtatgatc
     3181 gtttctatgg cggtgatgat ggaaaaaagt tatttgagtt tgccttaaaa agcacattca
     3241 aatcattagc accaaagcat aaagcttgtg agataccaca aataaatgag gatttaaaac
     3301 ataaacttga agaaattatg cagaaactta aaaacgattc ggagcatgaa aatgacagtt
     3361 aaatacaaca caattattaa aacaatgcta ccagtattca tgtattcaat actgtttatg
     3421 attaccgcaa caggtacaat ctatgctcaa aatccattaa cactaaaaga agccatagat
     3481 atagcaatca agaataataa cacctataaa atagccctgt tgaaggtaga agaaaaccga
     3541 tataaggtgc gcgaaagctg gggaatgtta tggccaaaac tttctactga tgtttcctat
     3601 acacgccaat ggtatgaagc tggtttccaa tcgcagattg aagggcaata taatgtgaaa
     3661 attattaatg gacagatatc tattaatccc ggtacatttt acaatacatt acaatcttca
     3721 cgtaacaatt ttattgccag cgaacatgat gcaaggaggg taaaggctga tacaactatc
     3781 acgacgctca ggctttacta ccagatattg ctgacaaagg aattgatcac catgcatgag
     3841 gaatcattaa aggcattgga ggaaaattta cgtatcgtga cggttggtta tcaaaagggt
     3901 acattctcaa aattggattt tttacgtgca agggttgcat ataacaatga aaaaacacaa
     3961 ttgatcaatg caaagaatga ttatttgacg gtgtgcgcta catttaatat tcatctaaac
     4021 cgggaaataa atgaagcggt tatactggac gaatctgttt taaaggtgca tgaagaggat
     4081 ttacaatacc ttgctcttga agaagccaaa aaaaatgaga tcgttgggca gcttgttgca
     4141 ttggctttga aaaatcgtcc ggaacttttg cagttaagct attctaaaac agcattggaa
     4201 tatgcaacaa gtgcaacctc atccatttac ctttggccca gtttttttgt aaacggtagc
     4261 tatggctcaa gcaagaacat atcaaaggat ggtggcggcg ggcttacacc aacaggtgat
     4321 ccagctacag accagatttt acaggctctg agccagggaa tgtcccagag ttttgctccc
     4381 agtggctgga acgacaactg gatgataacc tttggtgcta cctatcagtg gggtggatgg
     4441 tcaccactgg atagcaccca tgcaaaagca aagcagttag aaagccagac aaatcaggta
     4501 gatttgcagc tagaggattt tgttaaaagc atacgtcttg atatccagag ggggttttta
     4561 aaattggaat cagcctcaaa ctccatctat tcacagcagg ggaatgtaga aaccgctgaa
     4621 gaatcattaa agactgctac tactcagttt agaaatggta ttattgataa ttcaaaattt
     4681 ttagaagcaa atgtagcgct tgttcaggct aaaacattgt atattcagtc attatatgat
     4741 tataaggtat cacaggccga attgaataag gcaactggta ttgattattt tac
//
LOCUS       DAOOJH010000139         4753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14145_length_4753_cov_9.839932, whole genome shotgun sequence.
ACCESSION   DAOOJH010000139 DAOOJH010000000
VERSION     DAOOJH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14145_length_4753_cov_9.839932"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /locus_tag="PLJ75_11785"
     CDS             complement(<1..2096)
                     /locus_tag="PLJ75_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD05935.1"
                     /translation="MERAIRSFVKRFYLTTTLVEYCIIIPIALIVIALSTDITVQQLY
                     SIFIVVAVVAAVALVTFPPVQKYMFKPFIQLADLIKEGKEADAKIKILVRERLYALPA
                     MRSFIGIVFWFCSISLVIIGMWIIHIDVETILVCLVVGTFILIAAGFALTVVPQMIVN
                     EFITKNDFINDFINYSAKKNISIWGSLRNTLTVSISSMLLLSILLVTSTSLLFIFKSR
                     IDEYGRQMTTNALAVKNDIIATLQIGLDMAGIAAQHNSIEALHSIKQNVEIYDIFVMD
                     NEANANVRKTLKNKVTGLLAASLGMDGIKNKKPFIGSFVFVPAIKKSLAICAYPGNNV
                     WYCIAYDLHTLFSKHYSHYRIGNRGYILLMDKNGIFLSHPHEQYIGKENLQQYDWGKK
                     AFATQSVVKYPWEGERKIIIVTISNAPAFSVAQTAYEQEIAMTIKNIILFIVMISAGI
                     IIAGIAASIVILTVSFKPFATLRDSIYSIAEGDISAAPEVIYGNEIGSIATSIKTLTS
                     KISAVIGQIKNSSEELSTASEQMSAGISALSDVASNQSASTQEITATIEEISANLDSI
                     VINTKEESKSLSLFSTHLQELSQSIFSFLLIMEESLNRTQTISSEAQQGQVALQQMNT
                     SMKKIVDSSHGIANVAQIIRGIADQVNLLALNAAIEAARAGEAGRGFAVVADEITKLA
                     DAIAQSLKDIDNLVRA"
     gene            complement(2159..3586)
                     /locus_tag="PLJ75_11790"
     CDS             complement(2159..3586)
                     /locus_tag="PLJ75_11790"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HPD05936.1"
                     /translation="MSKKFIALLIVVIAIAGFVIYRAFAPATFDTAITYIDDGRYAKA
                     LRVLDSLSKTANYEEGERIYYYRLKALLGLINELNSDYEDERKTIDKRKDNKLIEKLK
                     RDIEKINKKEGLDCILVTDPKCYIFTGGKLYDEFIALYPGSRYRESIDFEILQFMTGV
                     HVNTIDPIIEFYKRYPETTYMSSLVNILFKALAGPSITVKEHTETLSSMLEKFCNKYA
                     SSAQYYRIFRCKGENVNLRDSSGTFGQITGKLAKGEIVVQLERSMDSVQIGDVRDYWY
                     RVVSVGGSRGWVFGKYLEHLPVKQVQAVEEEKFTIDERFTTWIDSSTPEGWQHYFHGY
                     ESAISFVKHGDQNIVKVNTQDKGGLYKKTGSFQNITCKVKGRLIQGNVILLGVVGYGG
                     YAYVITLMPKSLDACGYHIPYDTSLWHEYEMRSEGKVFSLYIDGELVARKISPVKHPL
                     LSSAGIYILICQDGGSAEVEYVKIK"
     gene            complement(3594..4751)
                     /locus_tag="PLJ75_11795"
     CDS             complement(3594..4751)
                     /locus_tag="PLJ75_11795"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPD05937.1"
                     /translation="MGELGDKTPLQYTDTRHMDYLAKNGICGLAHTVPHGMPPGSDVA
                     NLSLFGYDPVQCYTGRAPLEALNMGIELGPDDVAFRCNIVTIDNAIMKDFSADHIDSA
                     FTKIVIDELAQQIQLKDIEFYAGVSYRNIVVIRHFTGDMPVTTPPHDIQGQAVNDYLP
                     MGDNSKLLNDIMKQASEVIAHSKVITEAKTRYRGNPTAIWLWGGGFKPTMEPLSKRFG
                     LYGFTISAVDLIHGIGKAAGLSPIYVEGATGYIDTNYDGKAAALIEAMNRANFVFLHV
                     EAPDESGHEGNLEHKLKAIEDFDAKIVGPVVNMLNRYGEFTVLVMPDHPTPLNLRTHT
                     GEPVPFVIYSPDINYRLQCNGFNEVDAKATGIVVDCAHTLLAMMVNNSFPW"
BASE COUNT         1423 a         1045 c          815 g         1470 t
ORIGIN      
        1 tttgctcgta ctaaattatc tatatctttt aaactctgag ctatcgcgtc agctaacttc
       61 gtaatctcat ctgcaaccac tgcaaatcct cttccagcct cacctgctcg ggcagcctca
      121 attgcagcat tgagtgccaa taaatttacc tgatcagcta taccacgaat aatttgtgca
      181 acattagcaa ttccatgaga actatcgaca atttttttca tacttgtatt catctgttgc
      241 aatgctacct gtccctgttg cgcttcactg ctgatggttt gcgtgcggtt caatgactct
      301 tccataatga gcaaaaaaga aaatattgat tgcgatagtt cctgtagatg tgtagaaaaa
      361 agagaaagac tttttgactc ttctttggta tttatgacta tggaatctaa atttgcagag
      421 atctcttcaa tagtagctgt tatctcctgc gtagaggcag actgatttga tgcaacatct
      481 gaaagtgcgc tgataccagc agacatctgc tcagaagctg ttgatagctc ctcggaagaa
      541 ttttttatct gccctatgac agcactgatt ttactggtca atgtttttat ggatgtagca
      601 atgctgccaa tctcattgcc atagatgact tctggtgctg ccgatatatc cccttcagct
      661 atcgaatata ttgaatcgcg caatgtagca aatggtttaa atgaaacggt gagtattaca
      721 atacttgcag caatccctgc tattattata cctgccgaga tcataacaat aaatagtatt
      781 atatttttta tcgtcatggc tatttcctgc tcatatgccg tttgagctac tgaaaaagca
      841 ggagcgttgc tgatagttac aataatgatt ttgcgctccc cttcccatgg atatttgaca
      901 accgattgag tggcaaatgc ctttttaccc caatcatact gctgaagatt ttctttgcca
      961 atatattgtt cgtggggatg tgataaaaag ataccgtttt tatccattaa taatatatat
     1021 ccacgatttc caatacgata atggctatag tgttttgaaa acagcgtatg cagatcatac
     1081 gctatacaat accatacatt gttaccagga taagcacata ttgctaacga tttttttatt
     1141 gccgggacaa aaacaaaact gcctataaat ggtttcttat ttttgatacc atccatcccc
     1201 agagaagccg ctaacaaccc tgttacttta tttttcagcg ttttacgtac atttgcattt
     1261 gcctcattat ccataacaaa tatatcataa atttctacat tttgctttat agaatgcaat
     1321 gcttcaatgg aattgtgttg agccgcaata cctgccatat caagtcctat ctgcaatgta
     1381 gcaatgatat catttttaac tgccagtgca ttggttgtca tttgccgccc atactcatct
     1441 atgcgggatt taaaaataaa taaaaggctg gtggaagtaa ccagtaagat cgatagcaaa
     1501 agcattgaac ttattgaaac cgtcaatgta ttccgtaatg atccccagat gctaatattt
     1561 tttttagccg aataattaat aaaatcgtta ataaaatcat tttttgttat gaattcattg
     1621 acaatcattt ggggaacaac agtaagggca aatcctgcag ctatcagtat aaaggtacca
     1681 acaacaagac acaccagtat agtctctaca tcaatatgta taatccacat accaattatg
     1741 acaagactaa tgctgcaaaa ccagaaaaca atgccaatga aagaacgcat agcaggcaga
     1801 gcataaaggc gttcccgcac aagaattttt atttttgcat cagcctcttt tccttctttt
     1861 atcaaatcag ctaactggat aaaaggctta aacatatatt tttgcacagg gggaaaagta
     1921 acaagggcaa cggctgcaac tactgcaact acaataaaaa tggaatagag ctgctgaaca
     1981 gttatatcag tactgagcgc aataactatg agggcaatgg gtattatgat gcaatactca
     2041 acaagggtgg ttgtaaggta aaacctcttt acaaacgaac gtattgctct ctccatcttg
     2101 cacctccata ctttctatct ataaaaaaat aaaattacta ttatctttag tttgtcaatt
     2161 atttaatctt tacatattca acctctgccg aaccaccatc ctgacaaatt aaaatataaa
     2221 ttcctgctga tgataaaagg ggatgtttta ccggtgaaat cttccttgca acaagttcac
     2281 catcaatgta taatgaaaac accttccctt ccgaacgcat ttcatattca tgccacaacg
     2341 aagtatcata cggaatatga tagccacatg catccagact ttttggcata agggttataa
     2401 catatgcata tccgccatat ccaacaacac caagcaggat gacattaccc tgtataagcc
     2461 tgcctttaac cttacaggtt atattttgga agcttcctgt ttttttataa agccctcctt
     2521 tgtcctgtgt atttaccttt acaatatttt gatcaccgtg ttttacaaaa cttattgctg
     2581 attcataccc atgaaaatag tgttgccacc cttcaggtgt gctagaatca atccaggtag
     2641 taaagcgttc atcaatagta aacttttctt cttcaaccgc ctgcacctgt tttaccggta
     2701 gatgctcaag gtatttacca aaaacccacc ccctgctacc acctaccgat acaaccctgt
     2761 accagtaatc cctgacatcg ccaatctgta ctgaatccat ggaacgctca agctgtacta
     2821 ctatctcacc tttagcaagc ttgccggtta tttgtccaaa tgttcccgat gaatctctca
     2881 ggttgacatt ttcgccttta cacctgaata tgcgatagta ctgagcactt gaggcatatt
     2941 tattgcaaaa tttttcaagc attgaagata gtgtttctgt gtgctccttt actgtgatgg
     3001 aaggacctgc caatgcctta aaaagaatgt ttaccagcga tgacatatag gtggtttcag
     3061 gatagcgttt ataaaattca ataataggat ctatagtatt gacatggaca cccgtcataa
     3121 attgcaaaat ttcaaaatca atgctttcac ggtagcgact tcccgggtat aaagcaataa
     3181 attcatcata tagcttcccg ccagtaaaaa tataacattt tggatccgtc accagaatgc
     3241 aatccaatcc ttctttttta tttatttttt ctatatcacg ttttagcttt tcaataagtt
     3301 tgttatcctt gcgtttatca atggttttcc gttcgtcttc ataatcacta ttcaactcat
     3361 taattaatcc cagcaacgcc ttcaaacggt aatagtaaat ccgttcacct tcttcgtaat
     3421 tagctgtttt tgacaaactg tcaagcactc gcagggcttt agcatatctg ccatcatcaa
     3481 tatacgtgat tgccgtatca aacgttgccg gggcaaaagc acgatatata acaaatcctg
     3541 caattgcaat aactacaatg agtaaagcaa taaacttttt tgacatagtg tcctcaccat
     3601 ggaaatgaat tgttcaccat catcgccagc agcgtatgtg cgcagtcaac aactataccg
     3661 gtggcttttg catccacctc attaaaacca ttgcactgca aacgatagtt tatatctggc
     3721 gaatagatga caaaaggaac tggctcgcct gtatgagtcc ttagattcag tggcgttgga
     3781 tgatcaggca taaccagtac ggtaaattcg ccatagcgat taagcatatt gacaacaggg
     3841 ccaactatct ttgcatcaaa atcttctata gctttcagct tgtgttcaag attaccctca
     3901 tgccccgact catcaggtgc ttctacatgc aaaaacacaa agttggctct attcatggct
     3961 tcaatcaagg ctgcggcttt accatcataa ttggtgtcaa tatagccagt tgcaccttca
     4021 acatatatgg gcgatagtcc tgctgctttc ccaatgccat gaatcaaatc aactgctgat
     4081 atggtaaacc cgtataaccc aaaccgcttt gacagaggtt ccatagtggg tttaaatcca
     4141 ccaccccata gccatattgc cgtgggattt cccctgtatc gggttttagc ctcagtaata
     4201 acctttgaat gagctatcac ttcagatgct tgtttcatta tatcattgag cagcttgctg
     4261 ttatcaccca taggaagata atcattaaca gcctggccct gaatatcatg aggcggcgtg
     4321 gttactggca tatctcctgt aaaatgccgt atcaccacaa tattacggta tgaaaccccg
     4381 gcataaaatt ctatgtcttt taattgtatc tgttgtgcaa gctcatcaat aacaattttt
     4441 gtaaacgctg aatcaatatg atcagcgcta aaatccttca taatggcatt gtctatggtc
     4501 actatattgc aacggaatgc aacatcatct ggaccaagtt caatccccat attcaatgct
     4561 tcaagcggtg ccctgccggt atagcactgc accgggtcat atccaaacaa tgaaagattg
     4621 gcaacatcac ttccaggagg cataccgtgt ggcactgtat gtgccaatcc gcaaataccg
     4681 ttttttgcca gatagtccat gtgcctggtg tcggtatact ggagaggtgt tttatccccc
     4741 agttcgccaa tgg
//
LOCUS       DAOOJH010000140         4629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14631_length_4629_cov_10.966769, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000140 DAOOJH010000000
VERSION     DAOOJH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4629
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14631_length_4629_cov_10.966769"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            540..1859
                     /gene="larA"
                     /locus_tag="PLJ75_11800"
     CDS             540..1859
                     /gene="larA"
                     /locus_tag="PLJ75_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HPD05938.1"
                     /translation="MKIDLPWGSSSIQIDLPESWEVIIPEHQLKELSPKKQKGKDIVA
                     KSIAQPIAAKALAKYSLKNKKVVIIVDDNTRPTPAHTFFDVILDALKKAGCNTRNNAI
                     VIPALGIHTPMTQQEMEVKIGKKNISQIQWENHDAFDKAKNIHFGKTSRGIDVYLNHH
                     LKDADLILLIGLVEPHLWAGFGGGLKNILPGVAYSETIGHHHAIIAEPPYRFNRVAVD
                     AEQNSFRLDLEETLNMIKGEIFCINVVLDTNKEIIASFAGHPIKCHREAVAFTKTICG
                     LPLQKKVDGIITNSFPMDINFKQSMKCVGNCLPALRPKGIVMGFLRAERGLDDIPMPE
                     KSTPLWLTRTILRTLGASRVLGFLEKIQPGLNVEEKFLTYYSMQLIRAHNLYFYVPSL
                     SDKEVKALGFFERFTSPQGVIERGLNHLGKKATVAVFKEGGSTFPLF"
     gene            1944..3086
                     /locus_tag="PLJ75_11805"
     CDS             1944..3086
                     /locus_tag="PLJ75_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05939.1"
                     /translation="MAQCKDILLILLFCILISCSNSDSSEAKTNSRFATATINADIVE
                     SAKFLAGKPLSPSSRLFQLTLDKRYVSYVDEIKKSWERLQKKNIDAIVKWRGENLPKK
                     YNKTIFYPFSGPDILNAVAFFPDGDDYIMFGLEPCGDIPAPYSLTKEQVFSGLWGLRA
                     SLNEILKINYFITNDMKKEVSTNEFNSMISIIMWFLEQLNYDVINVKNIWIDDYSMVS
                     TMPATTQPNHYIPGAEIYFKDASGYIRRVRYFQINVIDNSLLQYTNFIPHLESYKRFT
                     TIIKSASYLMHNDTKFTKIRDITLKHSDLILQDDSGIAFKFFSPYEWKFTFFGVYNTP
                     IQVFANRYQQELKTRFNALSKGPLPFSYGYNKGTGRSNLMLAERIQ"
     gene            3083..3946
                     /locus_tag="PLJ75_11810"
     CDS             3083..3946
                     /locus_tag="PLJ75_11810"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPD05940.1"
                     /translation="MKKFSSAIIAGYIFILNCLPQLQAQSPIQNGIHTLYLPDAVEKQ
                     SKTIPVDIFIGDSNYQKNILVLPGWNFSRKRWIQETSLQQEALKRHYNLILPEMGITI
                     YESKYFNETKRKWAKTPGSEWIKTILIPEMQKRGLLLSHQFNAVMGLSTGGRGAVLTA
                     LYNKQIFKACASLSGDYNQATMSNDKLMTATYGPYSQFKDRWAKEDNPYFMIDGWNIP
                     LYLGHGTKDTVVPFDQTKAFYEKLKSQKPDITIIFHQCEFGHDFKYWSSEVVPILHFF
                     DAIYEHAGEQQ"
     gene            3943..4278
                     /locus_tag="PLJ75_11815"
     CDS             3943..4278
                     /locus_tag="PLJ75_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HPD05941.1"
                     /translation="MNDIDDIKQKAYDLAESIAQHPIAIRYFSLLEQMNEDEEAQQLL
                     TRLVNHGKKLNTMAEAGDTDIVNDPDSIELRNAIIEKPLVKEFVSAQNEYLGLIKEIM
                     EILHTRLNP"
BASE COUNT         1552 a          856 c          806 g         1415 t
ORIGIN      
        1 atcgcttaaa agagatgaat accgataact atcctaacta ttatgaaagc agggcgtaca
       61 ccttttcttt ttataaggaa aatggcatac attgctgcca ataccactgg ggaccacctc
      121 cagaagtact ccagcagaac gcttagttgc tgattatact tttatttttg tatattgcca
      181 taaagctaat tataatagca cggaaatatg ctatagtaaa cttcttttaa attattatag
      241 ccttaaaaaa actatttctt tgtcatgtca acaactgcaa aagcaggttt taccactgtt
      301 taatattcgc aggtgtattt acaattttaa attttcaatg ttccagcata atttattaat
      361 cacatgttct ttttatttca tagttttata gcttcaacat accagcagca ttcaatcatg
      421 cgacatcctc taaagaaggt atatttgata ttacaaatat taatatttct gttgacatat
      481 cataacaata ctatatacat ttaaaattaa atttattatt ttattatagg gggttcgtta
      541 tgaaaataga tttaccatgg ggttcttctt cgatacagat agatcttccc gaatcatggg
      601 aagttattat acctgagcac caattgaaag aactatcgcc aaaaaaacaa aaaggaaaag
      661 atattgttgc caaatcgata gcccaaccta ttgcagcaaa agcacttgca aaatattctt
      721 taaagaataa aaaagttgtc attattgttg acgacaacac acgcccaaca cctgcccata
      781 ccttttttga tgtgatactt gatgcgttga aaaaagcagg ctgtaacaca aggaacaatg
      841 ccatcgtaat acctgcattg ggtatacata ctccaatgac acaacaggaa atggaggtaa
      901 aaattggcaa aaaaaatata agccagatac aatgggaaaa tcatgatgcg tttgataaag
      961 caaaaaatat ccattttggt aaaacttctc gtgggataga tgtatatctt aatcatcatc
     1021 tcaaagacgc tgatctaatt ttactcatag gtcttgttga acctcatctg tgggctggtt
     1081 ttggcggagg gcttaaaaat attttgccag gtgttgctta ttcagaaact atcggccacc
     1141 atcatgccat tattgccgaa ccaccatatc gattcaacag ggtagcagtt gatgctgaac
     1201 aaaattcgtt tcgccttgat ctggaagaaa cgttaaatat gattaaaggg gaaatattct
     1261 gtattaatgt tgtgctagat accaataagg agatcattgc aagctttgct ggtcatccaa
     1321 ttaagtgcca tagagaagca gttgcattta cgaaaacaat atgcggcttg cctctccaaa
     1381 aaaaggttga tgggattatc accaattcat tccctatgga tattaatttt aaacagagta
     1441 tgaaatgtgt tggtaactgt ctgccagctt tacggccaaa gggcattgtt atgggctttt
     1501 tgcgtgctga acgcggactt gatgatatcc ccatgccaga aaaatctaca ccattatggc
     1561 ttacccgcac tattttaaga acattggggg cttcacgtgt actgggtttt ttagaaaaaa
     1621 tacaacccgg acttaatgtt gaagaaaaat ttttaaccta ctattcaatg caacttatac
     1681 gtgcgcacaa tctttatttt tatgttccat ctctctcaga taaggaagta aaagccctgg
     1741 gattttttga acgatttacc tcgccacaag gagtaattga gcgtggatta aaccatttag
     1801 gtaaaaaagc aaccgttgct gttttcaaag aaggtggctc aacttttcct ttgttttaag
     1861 tatcgcacaa taaagtaatt gacataaaaa gagcattgca caataccaat gccaattcaa
     1921 cgttaatagg acaaaggtga tctatggctc aatgcaagga tattctattg atactcctat
     1981 tctgcattct tatttcatgc agtaatagcg attcttcaga ggcaaaaaca aattcccgtt
     2041 tcgcaactgc aactatcaat gctgatattg tagagtccgc taaattttta gcaggcaaac
     2101 cactatcacc gtcatcacgt cttttccagc ttactcttga caaacgttat gtaagctatg
     2161 ttgacgaaat aaaaaagagc tgggaaagac tgcagaaaaa gaatatagat gcaattgtta
     2221 aatggcgtgg tgaaaatttg ccaaaaaaat acaataaaac aatattctat ccatttagcg
     2281 gacctgacat cttaaacgct gttgcctttt tccctgatgg tgatgactat attatgtttg
     2341 ggctggaacc atgtggtgat atacctgctc cctattcatt gacaaaggaa caagtttttt
     2401 caggtttatg gggattgcgt gcatcattga atgaaatatt aaaaataaat tattttatta
     2461 ccaatgacat gaagaaggag gtaagtacca acgaatttaa tagcatgata agcattataa
     2521 tgtggttttt ggagcaattg aattatgatg tcattaacgt aaaaaatata tggatagatg
     2581 attacagcat ggttagcaca atgcctgcta caacacaacc aaatcactac ataccaggcg
     2641 ctgagatata ttttaaagat gcttccggtt atatacggcg cgtacgctat tttcagatta
     2701 atgttatcga caattcattg ttgcaatata ccaattttat tcctcatttg gagtcatata
     2761 agcgctttac cactatcata aagtcagcat cgtatcttat gcataacgat actaagttta
     2821 ctaagatacg tgatataaca ttaaagcata gtgatcttat tctgcaggat gattcgggta
     2881 ttgccttcaa gttttttagc ccctatgagt ggaaatttac attctttggc gtatataata
     2941 caccaattca agtgtttgcc aatcgctatc aacaagagct caaaacacgc tttaatgcac
     3001 tatccaaagg accgttgcca ttttcgtatg gctataataa aggcactgga agatccaacc
     3061 tcatgttagc cgagcgcatc cagtgaaaaa attttcctca gctattatag caggttatat
     3121 ttttatttta aactgcctgc ctcagctcca ggctcaaagc ccaattcaaa atggcatcca
     3181 tacattatat ttacctgatg cagttgaaaa acaaagcaaa actattcctg ttgatatttt
     3241 tataggtgat agtaactatc aaaaaaacat tcttgtttta ccaggatgga atttttcgcg
     3301 caaacgatgg atacaggaaa cctcgttaca gcaggaggca ttgaagcgac actacaacct
     3361 catactcccc gagatgggaa taactatcta cgaatcaaaa tattttaatg aaacaaaacg
     3421 caaatgggca aaaacgcccg gctcagagtg gataaaaaca atactcattc cagaaatgca
     3481 aaagcgtggt ttattattgt cgcaccagtt caatgctgtt atgggactct ctactggtgg
     3541 acgcggtgcg gtattaactg cactttacaa caaacaaata tttaaagctt gtgcttcatt
     3601 atccggtgac tataatcagg ctacgatgtc caatgataaa ttaatgacag ctacttatgg
     3661 tccatactct cagtttaaag atagatgggc aaaagaggat aatccgtatt tcatgataga
     3721 cggatggaat atcccactgt atcttggaca tggcacaaag gatacagttg taccatttga
     3781 tcaaacgaaa gcattttatg aaaagttgaa atcacaaaaa ccagatatta ccattatatt
     3841 tcatcagtgt gaatttggcc atgattttaa atactggtct tctgaggtag tgcctattct
     3901 tcacttcttt gacgccattt atgaacatgc aggggaacaa caatgaatga cattgacgat
     3961 ataaaacaaa aggcatatga tttagctgaa agcatagcac agcatcctat agctatccgt
     4021 tatttttctc tcttagaaca gatgaacgag gatgaggagg cacaacaact actaactcgt
     4081 ttagtaaatc atggtaaaaa gcttaataca atggctgaag ctggtgacac ggatattgta
     4141 aatgacccgg attctattga attacgcaat gcgataattg aaaaaccttt agtcaaagaa
     4201 tttgtatctg cacaaaatga atatctggga ctcataaaag aaattatgga aatactacat
     4261 acccgattaa atccataatt tcatgctctg acaccatctt tttcaacata ctatgtcatg
     4321 gatgatattt ttttgcactc tgaaggctct gaccccaatt tctttctcat tttctcaatt
     4381 gtaatccgat tttcaacgtg atatatatat gtacttacgc aggcacagaa catgtgctct
     4441 gaccccttgt agatattgtt ttttcaaaaa aaatcacaaa aagtataaaa aaatacacca
     4501 tttcttttta atatttttca tctccggcgt tttatatagt atagagtagc aacatctatt
     4561 tttataaaaa atatggagga atacccatgg cacggaaatt gcgcctacaa ctgcccgagc
     4621 atacctacc
//
LOCUS       DAOOJH010000141         4621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14661_length_4621_cov_10.458826, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000141 DAOOJH010000000
VERSION     DAOOJH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14661_length_4621_cov_10.458826"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLJ75_11820"
     CDS             <1..816
                     /locus_tag="PLJ75_11820"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPD05942.1"
                     /translation="MAPYYLEKARFGYRMGDSPLKDHMVHDGLWDIVNDFHMGISNEL
                     CSEKYGISRQEQDRYAEASYTKAMHAIASGAFKKEIVPVTIAQKQRTVVFDTDECPKE
                     TPYEVLAAMKPSFKAGGVTTAGNSSVISDGAAALVVMSKEKADTLGCTIMATVGAQAC
                     AGIDMKYVLVAPIWAIPKCCKKEGVKLEDIELYEVNEAFSGSSVAVLKELKLDAGKVN
                     VNGGSVALGHPIGASGARVLVTLVHEMIARDKKSGLASLCLGGGEAVALIVKR"
     gene            834..>1471
                     /locus_tag="PLJ75_11825"
     CDS             834..>1471
                     /locus_tag="PLJ75_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPD05943.1"
                     /translation="MQVNDIKVFGVVGAGQMGHGIACVAALSGLTVILNDISMEYVNK
                     GIQGIDAVLTRNVDKGTLTHEEKDAVLHRIKPCTALNDLADADFIVEAASENPQIKWK
                     LFSDLDALCPSHVVLATNTSSIPIGRIASVTRRADKVIGMHFMNPVPVMKLVEVIRAL
                     ATSDETFDVTWNLSIKFGKTPALANDYPGFIANRILMPMINEAIYSLYEGVGT"
     assembly_gap    1472..1571
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1572..2573
                     /locus_tag="PLJ75_11830"
     CDS             <1572..2573
                     /locus_tag="PLJ75_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPD05944.1"
                     /translation="VDPRYGGTGAGSVAFSLVLTQLGKACAATAVTTSVTNMVAEVIQ
                     SVGTEEQKCRYIPALCDGTFYAGAFGLTEAEAGSDPSAMKTTAVPDGNSWILNGRKVF
                     ITSAEYAGIVVVWAVTDTSAPRGRGISAFLVEPGFQGFAVGKDESKMGQLGSSTNEII
                     LEDCRVPRENLLGNENEGFKIAVKELAGGRIGIGSMALGIGLAAMDFATGYANERVQF
                     DVPIIQHQAIQWMIADNYTSLEAARLLLLKAAYLKENNKPFMKEASMAKLFATEAANK
                     ACYDAMQILGGYGYTKDYPIERMYRDVRVTSIYEGTSQIQRLIIAKSYMDDAKVNAG"
     gene            2744..>2958
                     /locus_tag="PLJ75_11835"
     CDS             2744..>2958
                     /locus_tag="PLJ75_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05945.1"
                     /translation="MGESFDSVIYVQEDSVSTIAFTGEELKVLERINQKIAAGESLVA
                     IIEFFFRETQHIMPCDRIGIAFSDDGK"
     assembly_gap    2959..3058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3059..3829
                     /locus_tag="PLJ75_11840"
     CDS             <3059..3829
                     /locus_tag="PLJ75_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPD05946.1"
                     /translation="QIALHHAMVERLSQAVEKAYRIEQLTRAINAYMEMLSFVTHELK
                     SPLSSIMTLGTTLQQGYFGQIPEHCKTTIERMMAQAEYLLTIVNEYLHLARLETGSMP
                     VKKSIIDIIKEVIVPSIGIVQPQADAMHITIQQDYNDDVKVEGDPDLLKIVMHNLLNN
                     AIKYNSDHGLIRITAKRQDGLCMIKVWNTGPGFPQEEKYKLFKRFSRIETEELLSRKG
                     SGIGLFISWNIVYMHNGRIYADSAQGQYAEFTVELPLP"
     gene            complement(3821..4498)
                     /gene="pncA"
                     /locus_tag="PLJ75_11845"
     CDS             complement(3821..4498)
                     /gene="pncA"
                     /locus_tag="PLJ75_11845"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HPD05947.1"
                     /translation="MTPLSSLANYGQTTIQKDDALLVIDMQYDFIPGGALAVAEGDAI
                     IQPISLLMEKFFTTGATVVCTQDWHPQGHHSFASAHGKNPYDPIEQPGIGPVLWPDHC
                     VIGTQGAMLHEDIAASYCHAIVRKGYTVTIDSYSAFLENDKKTPTGLHGYLHDRGIHR
                     IFVCGLAFDYCVQFTAVDGSNQNFEAIVIVDCTKAVHSPSTSVEDAIATMKQHAVQFA
                     LSRDMSW"
BASE COUNT         1315 a          824 c         1111 g         1171 t
ORIGIN      
        1 atggcaccct actatttaga aaaagcccgg tttggttatc gtatgggcga tagtcccctg
       61 aaagatcaca tggttcatga tggattgtgg gacattgtca atgattttca tatggggata
      121 tccaatgagt tgtgctcaga aaagtatgga atcagcaggc aggagcagga tcgatatgcc
      181 gaagcgtcat atacgaaagc aatgcatgct attgcatcag gtgcatttaa aaaagagata
      241 gtcccggtaa ctatcgccca aaaacaacgc acggtagttt ttgatactga tgaatgtcca
      301 aaggaaacac cgtacgaagt tttagctgcc atgaaaccat cgtttaaagc aggtggggtt
      361 acaacagctg gcaatagttc ggttatcagt gatggtgctg ctgcactggt ggtgatgtca
      421 aaagaaaagg cagatacgct gggatgcacc atcatggcta ctgttggagc gcaggcatgt
      481 gctggcattg acatgaagta tgtgctggtg gcgccaatat gggcaatacc aaaatgctgt
      541 aaaaaagaag gcgtaaagct ggaggatatt gagctttatg aggttaatga agcgttcagc
      601 ggctcaagtg ttgcagtgct gaaagaatta aaacttgatg ccggcaaagt caatgttaat
      661 ggcggcagcg ttgcattggg gcatcctatt ggtgcaagtg gtgcacgggt gctggtgacg
      721 cttgtccatg aaatgatagc gcgagacaaa aaatcagggc ttgcctctct gtgtctgggt
      781 ggaggcgagg ctgttgcatt aattgtaaaa cgataactaa aaggataaac gcaatgcagg
      841 taaacgatat aaaagtattt ggtgttgtag gtgcagggca gatgggtcat ggcatagcct
      901 gtgtggcagc gttaagcggg cttaccgtta tactgaatga cattagcatg gagtatgtta
      961 acaaaggtat tcagggtata gatgcagtgt tgacacgaaa tgttgataag ggcacattga
     1021 cacatgagga aaaagatgca gtgctgcata ggattaaacc gtgcacagca ttgaatgacc
     1081 ttgcagatgc agattttatt gttgaagctg ccagcgaaaa tcctcaaata aaatggaaac
     1141 tatttagcga tttagacgcg ctatgcccgt cgcatgttgt attagctacc aatacctcat
     1201 ctatccccat tggacgtatt gcatcggtaa ccagacgggc tgacaaagtt attggcatgc
     1261 actttatgaa tcctgtgccg gtaatgaagc tggttgaggt aataagggca cttgccacat
     1321 ctgatgaaac ctttgatgta acatggaatt taagcataaa gtttggtaaa acgccagcat
     1381 tggctaatga ttatcccggc tttattgcca acaggatact tatgcccatg ataaatgagg
     1441 caatctatag cctttatgaa ggggtaggga cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn ngtagacccc cgctatggcg gaacaggtgc cggcagtgtt gcattttcac
     1621 tggtgctgac acagctgggg aaagcatgtg cagctacagc cgttacaaca tcagtgacga
     1681 acatggtggc agaggttatt cagtcagtgg gtacagaaga gcaaaaatgc aggtatatcc
     1741 ctgctctgtg tgatggcact ttttatgcag gcgcctttgg tttaaccgag gcagaggcgg
     1801 gctctgaccc ctctgctatg aaaactactg cggtgcccga cggcaacagc tggattttaa
     1861 atggcagaaa ggtatttatt accagcgccg aatatgccgg tatagttgtt gtctgggcag
     1921 ttactgatac aagcgcgccc agagggagag gaatcagcgc ttttctggtg gagcccggct
     1981 ttcaaggatt tgcggtgggt aaggatgaaa gcaagatggg gcagttaggt tcatcaacca
     2041 atgagattat cctggaagat tgcagggttc ccagggaaaa tctgcttggt aacgaaaatg
     2101 aagggtttaa gattgcagta aaagagctgg ctggcgggcg catagggata ggttctatgg
     2161 cgctggggat tggactggct gctatggact ttgctacggg ttatgcaaat gagcgtgtac
     2221 agtttgatgt acccattata caacatcagg caatacagtg gatgattgct gataactata
     2281 ccagtctgga ggcggcgcgg cttttacttc taaaggcggc ttacctcaag gaaaataata
     2341 agccgtttat gaaggaagca tcgatggcaa agctatttgc aaccgaggca gcaaacaaag
     2401 catgttatga cgccatgcag atactgggcg ggtatgggta tacaaaagat tatcccattg
     2461 agcggatgta tcgcgatgtg cgggtaacat caatttatga aggaacaagt caaatacagc
     2521 ggcttattat tgctaaaagt tatatggatg atgcaaaagt aaacgctggt taactatata
     2581 ctgcaggcgg taaaggatat attaatgggc gatagttacc gtcaattgat attctgtcca
     2641 ataactatcg cccttataga taatagtcaa actataaaaa atcttgcata atttttccat
     2701 gctgtcacga tggtaacatt accatataat aaagggccga tgcatgggag aatctttcga
     2761 ttcagttatc tatgtacagg aagacagtgt cagcaccatt gcctttacgg gtgaagagtt
     2821 aaaagtttta gaacgtatca atcaaaaaat tgctgcgggg gagtctcttg tagctattat
     2881 agaatttttt ttcagagaaa cacagcatat tatgccgtgt gacaggattg gcattgcatt
     2941 ttccgatgat gggaagagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnca
     3061 gatagcgctc caccatgcta tggtggagag gttaagtcag gcggttgaaa aagcgtaccg
     3121 tattgagcag cttacccggg caataaatgc ttatatggag atgctgagct ttgtgacgca
     3181 tgaactgaaa agcccgcttt cttctatcat gacattgggc acaacattac agcagggata
     3241 ctttggccag atacccgagc attgtaaaac caccatagag cggatgatgg ctcaggcaga
     3301 ataccttttg acgatagtca atgaatattt acatttagca cgtcttgaaa ctggctccat
     3361 gccggtgaaa aaatctatta tcgacattat aaaagaagtt attgtacctt ccatagggat
     3421 tgtgcaacca caggctgacg caatgcatat cactatacag caggattaca atgatgatgt
     3481 aaaggtagaa ggtgatcccg acttattgaa gatagtgatg cataaccttt tgaacaatgc
     3541 tataaagtat aatagtgatc atggattaat acgtattaca gcaaagcggc aggatggatt
     3601 atgcatgata aaagtttgga acacaggacc tggattccca caggaagaaa aatataaact
     3661 ttttaaacgc ttttcacgaa tcgaaacgga agaattactt tcgcgtaaag gttctggtat
     3721 tggacttttt atttcatgga atattgtgta tatgcataat ggcaggatat acgctgattc
     3781 ggcacaaggg cagtatgcag agtttaccgt tgaattgcca ttaccatgac atatccctgg
     3841 acaaagcaaa ttgaacagca tgttgtttca tggtagctat tgcatcttct acactggtag
     3901 atggtgaatg aaccgctttt gtacaatcaa caataactat cgcctcaaaa ttttggtttg
     3961 aaccatcaac cgctgtaaac tgtacacagt aatcaaatgc aagcccgcat acaaatatac
     4021 ggtgtatgcc tctatcgtga agatatccat gcaaaccggt tggcgttttt ttatcgtttt
     4081 ccagaaatgc agagtaacta tcgatagtca cagtatagcc cttacgcacg atggcatgac
     4141 agtagctggc tgcaatatct tcatgaagca ttgccccctg tgtaccaatg acgcaatgat
     4201 cgggccagag cactggtcca atgcctggtt gctcaatggg gtcataagga tttttgccgt
     4261 gggctgatgc aaatgaatgg tgcccctgcg gatgccagtc ctgagtgcaa acaaccgtag
     4321 cacctgttgt aaaaaatttt tccataagca aagagatggg ttgtataatt gcatcaccct
     4381 cagcaacagc aagcgctccc ccggggataa agtcatattg catgtcaatg actaacagcg
     4441 catcatcttt ttgtatggtg gtttgcccat agttagcaag gctggataac ggtgtcatgg
     4501 ttgccctcct ttatgtcatc atagaaacgt cataatacac tatcacagta agagtaatac
     4561 catatagagc atgtggaatt gcaagttttt tttcaaagac atgcaatgaa gatttatcaa
     4621 t
//
LOCUS       DAOOJH010000142         4584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14805_length_4584_cov_9.330757, whole genome shotgun sequence.
ACCESSION   DAOOJH010000142 DAOOJH010000000
VERSION     DAOOJH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14805_length_4584_cov_9.330757"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="amrS"
                     /locus_tag="PLJ75_11850"
     CDS             complement(<1..618)
                     /gene="amrS"
                     /locus_tag="PLJ75_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPD05948.1"
                     /translation="MKEAQYYHLHGEDSVQCVLCPHNCIIKEGKTGICGVRQNKGGTL
                     YSLIYEKPCAVHVDPIEKKPLYHFLPGSRSYSLGTVGCNLQCMHCQNWELSTSLPGEV
                     FCNTLTSDDCVRLALQYECASISYTYNEPGINFEYVLETAIKAHEKGIKNVMVTNGYI
                     NEEPLRELYQHIDAANVDLKAFDDDFYKTVCKARLQPVLKTLTILK"
     gene            complement(634..2289)
                     /locus_tag="PLJ75_11855"
     CDS             complement(634..2289)
                     /locus_tag="PLJ75_11855"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HPD05949.1"
                     /translation="MKSFKKAVVVLIIGIACIANAKPGKKVIGVTAFTNLSKDTAYDW
                     LCIGIADTISYKLRNVQEYILVDRVNVDKIIGELQLSQSGLIDENTVEAGKALGASIL
                     VVGNFQKSGNQIRITAKIVDVQSHAVINQVQVTGTINDIFNLQDSIALGIIDKNDTAI
                     TTETKNKIVQNDTNNISAYEYFVRGQKYLLYQLNYSKAIEMYQKAISIDANYAIAYAG
                     LGKAYSLRSWELREYYNTTDTTLIDKSFQSSKKALALNPNLDEAHLSLARYYQEVDEK
                     KITNKWTLCEEETRKALEINENNGEAYLLLSKIFAYDIPKSEMYLKQAIEKNKFLVDA
                     HNNLGIIYLDRGDLDTAYSYIKNAIDIDDNYVIGYMNLGVIYEKRGQYDKALEMYKMA
                     VSKYPNYIQGLRNLGIGYRNLNRLDEALETFFKALKLKKDDAQTLSEIGYVYLLKAEY
                     NKATDYFKQSLRYDPSYRYSLANMGWCLVQLGKNNDAVPYLEKAHNDNSDYAWPAGYL
                     GWLYENKFQDTNKALYWYGEALKRDPNNQDYRNAYNRLSGNYR"
     gene            complement(2438..>4584)
                     /locus_tag="PLJ75_11860"
     CDS             complement(2438..>4584)
                     /locus_tag="PLJ75_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPD05950.1"
                     /translation="YPETITPYTGGIVGMALYRVLITIMGLTGTWIVTITLMLISLLL
                     LGIVSLSYILNNNSIDSLAKIFAIIYKKIIIFKQKDSIPLIDIKHFDSDKKLPWITKT
                     KITIYNKTGKKKLKLIEYDNILNDNNTIPAAIDDLNKLSVIMPLKDISSIHGMGDTTN
                     GYVQQHIETAYIVPDAENEQVPETTNGTNIAVKIKDDISQSRGTIALPESDEIAENTM
                     PDISENDIYDEQVNEAISNNEDMVARNVFEAIPINQHYSIPVDYLRQYNSHDETGYKQ
                     EVVKNSELLVNTLKDFGIESRVVNVNRGPVITLYELQIAPGIKVNRIAGLADDIAMAL
                     AASRVRVVAPIPGKKAIGVEIPNKVRETVTVKDIITSQEYAHQSGRLKVGLGKDIMGK
                     PVIIDLKKLPHLLIAGATGAGKSVCVHSIITSLVYNYDPNYVRFIMVDPKLVELQVYN
                     GIPHLLTPVIIEPFMVPKALKWTMLEMERRYYLLSEYNTRDIDRYNQMVERNKSGEYL
                     PYIVIIMDELADLMMVAAKEIEGYITRIAQKARAVGIHLVLATQRPSVDIITGIIKAN
                     FPARIAFQVAQKTDSRTIIDQNGAEKLLGKGDMLYQSPMSSFPIRIQGAYISEDELSS
                     VVAFLKKIANPHYVDIEQSLVEFDDDDEESEDDLFIEAVKIVETTKKASASYLQRRLS
                     IGYNRAARIIERMEELGYIGPQQGSKPREVYI"
BASE COUNT         1304 a          902 c          827 g         1551 t
ORIGIN      
        1 tttaaggatg gttaatgttt ttaaaacagg ttgcagcctt gctttgcata ctgttttata
       61 aaagtcatca tcaaatgcct ttagatcaac atttgccgcg tctatgtgct ggtacagttc
      121 acgcagaggc tcttcattga tatagccatt ggtaaccatc acatttttta tacctttttc
      181 atgggctttt atagcagttt caagaacata ctcaaagttt atccctggtt cattataggt
      241 atacgatata gaagcgcatt catattgcaa cgctaacctt acacaatcat cagaggtaag
      301 cgtattgcaa aaaacttctc caggcaggct ggtagataat tcccagtttt gacaatgcat
      361 gcattgaaga ttacatccta cggtgcccag tgaatacgac ctgctccctg gtaaaaaatg
      421 gtacagtggt tttttttcta tggggtcaac atgtactgca caaggttttt cgtagatgag
      481 tgagtatagt gtgccacctt tattctggcg tacaccgcat atacctgttt tcccttcctt
      541 gatgatacaa ttgtgaggac ataatacaca ttgcacactg tcctcaccgt gtaagtgata
      601 atactgagcc tctttcatga agcgtttaaa cctttagcga tagttaccag ataaacggtt
      661 atatgcatta cggtaatcct gattattggg atcacgcttt aaagcttcac cgtaccagta
      721 gagagctttg tttgtatcct gaaatttatt ttcataaagc cagcctaaat atcctgctgg
      781 ccatgcatag tcgctattat cattatgagc tttttctaaa tagggtactg cgtcattatt
      841 tttgcccagt tgaaccagac accatcccat gttagctaaa ctataccggt atgaaggatc
      901 ataccgtaac gattgtttaa agtaatctgt tgctttgttg tattcagctt ttaatagata
      961 tacgtatcca atctcgctta atgtttgtgc gtcatccttc tttaacttta aagctttgaa
     1021 gaaggtttcc agagcttcat caagcctgtt tagattacgg tatcctatcc ccaaatttct
     1081 aagcccctgt atgtagttag ggtattttga tacagccatt ttgtacattt ccaatgcctt
     1141 atcatactgt ccgcgttttt cataaatgac acctaaattc atatagccta taacatagtt
     1201 gtcatcaata tcaatggcat ttttgatgta tgagtatgcg gtatccagat ctccccggtc
     1261 caggtagata atccctaagt tattatgtgc atccactaaa aacttattct tttcaattgc
     1321 ctgttttaaa tacatttcag atttaggaat atcataggca aaaattttac tgagcagtag
     1381 atatgcttca ccattatttt cattaatttc taaagccttt cgggtttctt cttcacataa
     1441 tgtccatttg ttggttatct ttttttcatc aacttcctga taataacggg ctaatgaaag
     1501 atgagcttcg tctaaatttg gattaagggc taacgctttt ttgctgctct gaaatgattt
     1561 atcaatgagt gttgtatcag tggtgttata atattctctc agctcccatg aacgcagcga
     1621 gtacgctttg ccaagcccgg catacgcaat agcataattg gcatcaatag agatggcttt
     1681 ctgatacatc tcgatggctt ttgaataatt tagctgatac aataaatatt tttgtcctcg
     1741 tacaaaatat tcataagcac taatattatt ggtatcattt tgaacaattt tattttttgt
     1801 ttcagtggta atagctgtat catttttatc aatgattccc agtgcaatgg aatcctgtag
     1861 gttaaaaata tcgttgatag ttcctgttac ctgtacctga ttaataacag catgtgactg
     1921 cacatctaca atttttgctg ttatgcgaat ttgattgcca gatttctgga aattaccaac
     1981 caccaggatg cttgcaccta acgctttgcc tgcttctaca gtgttttcat ctataagtcc
     2041 cgattgcgaa agctgtaact cgccaattat cttatcaaca ttaacgcggt ccacaagaat
     2101 atattcctga acatttctga gtttatacga aatagtatca gcaataccta tacatagcca
     2161 gtcatacgct gtatcttttg aaagattggt aaatgcagtg acacctatta cttttttgcc
     2221 tggtttagcg ttggctatac atgcaatgcc aattatcaaa acaactaccg cttttttaaa
     2281 ggatttcata gaccctctca tactgataaa tgtgggatga tattaatatt aaaatgaagc
     2341 gtattgtatc attacatttt tctaaaataa caataaagta actttattta tagataatat
     2401 actaaaggtg gttgaaaaag tcatgcaaaa aattaattta tatatatact tctcgtggtt
     2461 tacttccctg ctggggacca atatagccta attcctccat ccgctcaatg atgcgggctg
     2521 ctctgttgta accaatagat aaacgtcgct gaagataaga agctgatgcc tttttagtgg
     2581 tttcaacaat cttcacagcc tcaatgaaaa ggtcatcttc actttcctca tcatcgtcgt
     2641 caaattccac aaggctctgt tcaatgtcaa cataatgagg attggctatt ttctttaaga
     2701 atgctactac tgatgacagc tcatcctcag agatataagc gccctgtatg cgtatgggaa
     2761 aagagctcat tggcgactga tagagcatat cgcccttccc cagcagcttt tcagcaccat
     2821 tttgatcaat gatggttcgg gaatcagtct tctgggcaac ctgaaatgct atccgagcgg
     2881 ggaagtttgc ttttatgatt ccggtaatga tatcaacgga cggccgctgg gttgccagta
     2941 ccagatgaat gcccacagca cgtgctttct gagcaatgcg tgtaatatac ccttctatct
     3001 ctttggcagc aaccatcatc aagtcggcaa gttcatccat aataataacg atgtacggta
     3061 gatattcacc gcttttattt cgctctacca tctgattgta gcggtcaata tcacgggtat
     3121 tgtattcaga aagcagatag tagcgacgct ccatctctaa catggtccac tttaatgctt
     3181 taggtaccat aaaaggctca ataatgacag gtgttaaaag atgaggaata ccattatata
     3241 cctgtaattc cactaatttt gggtcaacca taataaagcg aacataatta gggtcatagt
     3301 tgtacaccag gcttgttatg atagaatgca cacataccga tttgcctgca ccggttgcac
     3361 cagcaataag aagatgggga agttttttta gatcaatgat gaccggtttc cccatgatat
     3421 ctttgccaag ccccacttta agacgtcctg attgatgagc atattcctgt gatgtaataa
     3481 tatcttttac agtaacggtt tcccgcacct tgttggggat ctctacgcca attgcttttt
     3541 tgcccgggat tggtgcaaca accctaacac gcgatgcggc aagcgccatg gcaatatcat
     3601 cagcaagccc tgcaatgcgg ttaactttaa tacccggagc tatctgtaac tcgtagaggg
     3661 taattacagg cccacgatta acgttgacaa ctcttgattc aatgccaaaa tctttaagcg
     3721 tattaacaag cagctcagag ttttttacta cttcctgctt gtagccagtt tcatcgtgtg
     3781 agttgtattg ccgtaagtag tccaccggaa tgctgtagtg ctgattgata ggtattgctt
     3841 caaatacatt gcgtgcaacc atatcttcat tattgctaat tgcttcatta acttgctcat
     3901 cataaatatc attttcactt atatcgggca ttgtgttttc agcaatttca tcagattcag
     3961 gtaatgcgat agttccccgg gattgggaaa tatcatcctt tatttttact gctatatttg
     4021 taccattggt tgtttcgggt acttgttcgt tttctgcatc tggtacgata taggctgttt
     4081 caatgtgctg ttgaacatat ccattggtgg tatcacccat accatggata gaagatatat
     4141 ccttcaatgg cataataacc gatagtttgt ttaaatcatc gatagcagcc gggatggtgt
     4201 tattatcatt aaggatatta tcatattcaa tcagtttaag ctttttttta ccagttttat
     4261 tataaatagt gatttttgtt tttgtaatcc atggtaattt tttatcagag tcaaaatgct
     4321 taatatcaat aagcggaatt gaatcttttt gtttaaagat gattattttt ttataaataa
     4381 tggcaaagat ctttgccaat gaatcaatgc tattgttatt taaaatataa cttaatgata
     4441 caatacccag taatagtaag gaaatcagca tcagggtgat agttactatc catgtaccgg
     4501 ttaatcccat aatagttatc agtacacgat ataatgccat tcctactata ccgcctgtat
     4561 aaggagtgat ggtttcagga tata
//
LOCUS       DAOOJH010000143         4533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_14998_length_4533_cov_10.060965, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000143 DAOOJH010000000
VERSION     DAOOJH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14998_length_4533_cov_10.060965"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLJ75_11865"
     CDS             <1..534
                     /locus_tag="PLJ75_11865"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPD05951.1"
                     /translation="LVLIFTARIFDVSIGTVRIILVARGYRSIAPVLGFFEVLIWLFA
                     INSALQHFNGIISYIVYALGFATGNYVGMLLEEKIAIGYQSVRVITSKVVSALPLVLR
                     QEGFGVSQIDGVGMKGPITMLFTVIPKRDVKRVVEIVQMLEPNAFITIEDVKQQLSGF
                     TIHNGFVSKFASLIKKK"
     gene            complement(610..2424)
                     /locus_tag="PLJ75_11870"
     CDS             complement(610..2424)
                     /locus_tag="PLJ75_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPD05952.1"
                     /translation="MIKGTTIDFAIIVIYFIVILGFGSFFGKYVKSTKDFFFGGQRFS
                     WWLIGFSCVATTVGSYSFIKYSAVAFEYGFSSTMTYLNDWFILPIFVFIWLPIIYYGR
                     ITSIPEYFEKRFDSKTRTISVIILLIYLLGYIGINLYTIGVAFNAMLGWDVMATAAVI
                     AFVCALYMHAGGQTSVIMTDLAQALVLLTAGILIFILGIDKIGSLKTFWTSLSYEFKL
                     PFSPFNQPPDFNFVGIFWQDAFGSSMAFYFINQGILMRFLSVRSPRDGRKALFLVCLV
                     LMPLAAFAVANAGWIGKAMVQSGLLSPNVDPNHIFVDVAHSISKPGIFGFIMAALIAA
                     LMSTIDTLINGVAAIGVNDIVKNIAPDNTDAYYLKWARLISLLAAIIGVALVPLFMQF
                     KSIYVAHGTFIAAVTPPMIVTILFGILWKRYTTQAATYTLIFGSIAMLISIKWPGILT
                     LFSHGVATEGLQYLRALYGLTICSGISIAMTLLPASKEIKDLPGLVVDTIHEGKVLFK
                     GGEPDDFDEGKIISSHIVEVNEGVSLSKAMMEHLQAKPGDILYISDERWWLGGLKSIH
                     AKAGPPHDSSDDIILIGTDLIVQGNLDINRKVTAMKII"
     gene            complement(2427..2627)
                     /locus_tag="PLJ75_11875"
     CDS             complement(2427..2627)
                     /locus_tag="PLJ75_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05953.1"
                     /translation="MMSPWLPFIYQYTICSIFFIAMIVLSIKKKAISIHHPTDRKILL
                     EIISGYLIFIFAHALMILIAGA"
     gene            complement(2624..>3091)
                     /locus_tag="PLJ75_11880"
     CDS             complement(2624..>3091)
                     /locus_tag="PLJ75_11880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPD05954.1"
                     /translation="AIAHCNELKSVEYIYTSDFLRAYETAMIIARHMNIINVKLDIRL
                     RERYFGQFEGTDSENYQRIWDMDCRNVNDPVCYDVEPIHTVAKRMISFLLDCETNHIN
                     DTILIVSHGDPLQILYTVSIGLPINKFKTASHFNNAELRLLPSRFTIDEELIT"
     assembly_gap    3092..3191
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3192..3892)
                     /locus_tag="PLJ75_11885"
     CDS             complement(<3192..3892)
                     /locus_tag="PLJ75_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD05955.1"
                     /translation="MKKKDLSMFVRFWGVRGSVPTPGINFIKYGGNTACVELRCGNTI
                     MIFDAGTGIRMLGESLAKEFFDKPIQVHLFISHTHWDHIQGFPFFALAYNKNATINFY
                     GGHSVSTLEKLILGQMDKEYFPVSLFELSSKINFIELSQNAFTINDIVIYYTHLFHPG
                     LSLGFRVEYDDRVFVYASDNEVLNDPELGEYNLKNIGTLIKNADILVTDCQYTPDEYK
                     NRIGWGHSSIEIVAQV"
     gene            complement(4000..4515)
                     /locus_tag="PLJ75_11890"
     CDS             complement(4000..4515)
                     /locus_tag="PLJ75_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05956.1"
                     /translation="MLLHRSKLPIIALVLSIACTNQTLSIMKANIGSNVALNGTITNA
                     SLHYTFTPCTWCSTAKDIWNYIYFQGDTLCFAVECTTNINDAIVTAYFENPATRKTYK
                     AERLEKINTTIYGFSLIGSLLEHIYNDTPMKVLCTKRVALPFVIHITVELKNQKATYS
                     IANTAYISFSQ"
BASE COUNT         1478 a          780 c          802 g         1373 t
ORIGIN      
        1 ttagtattaa tatttactgc cagaatattt gatgtgtcaa ttggaacagt gcgaataatt
       61 ttggtggcaa gaggataccg atctatagcc ccggtattgg gattttttga ggttcttatc
      121 tggctttttg cgattaattc tgcacttcaa cattttaatg ggattataag ttatatagtt
      181 tatgcattag gttttgccac aggaaattat gttgggatgt tattagagga aaaaatagcc
      241 attgggtacc aatcagtccg agttattacc agcaaggtcg tttctgcact tcctctggta
      301 ttgaggcagg agggttttgg agtaagtcaa attgatggtg ttggcatgaa agggcctata
      361 acgatgttgt ttaccgtcat accaaaaaga gatgtgaaaa gagtggttga aatagttcaa
      421 atgttagaac ccaatgcatt catcaccata gaagatgtta aacagcaatt gagtggtttt
      481 actattcata atggatttgt atcaaagttt gcttcattaa taaagaaaaa ataatttata
      541 tttttttgat ctatacattt aaaaacaaaa attatatatt ctatgttttg tagagggcga
      601 tagttactat caaattattt tcattgctgt aaccttgcga tttatatcga gatttccctg
      661 cactataaga tcagtgccta taagaatgat atcatcactg ctatcatgag gtggaccggc
      721 tttcgcatga atggatttaa gtccacccag ccaccaccgt tcatctgaaa tgtacagtat
      781 atcgccaggt tttgcctgca ggtgttccat cattgctttg cttagtgaca caccttcatt
      841 tacttcaaca atatggctgg agattatttt tccctcatca aaatcgtcag gttcgccgcc
      901 tttaaataaa accttacctt catggatagt atctaccaca agaccgggca ggtcttttat
      961 ttcctttgag gcaggtaata atgtcatggc aatgctgatt cctgaacata tggtaagtcc
     1021 gtataaagct cgcaagtatt gtagtccctc ggtagcaaca ccgtgtgaaa ataacgttaa
     1081 tattccaggc catttaatgg atatgagcat tgctatgctt ccaaaaatta atgtatatgt
     1141 ggcagcttgt gtggtgtatc ttttccataa aataccaaat agtatggtaa ctatcatggg
     1201 tggagtaaca gcagcaataa acgttccatg agcaacatag attgatttga attgcataaa
     1261 taagggaaca agagcaacac caataattgc agctaataac gatattaaac gtgcccattt
     1321 gagatagtaa gcatcagtgt tatcgggtgc aatatttttt acaatgtcat taacaccaat
     1381 tgcagcaaca ccgttgatta atgtgtcaat ggtagacata agcgcagcaa ttaaagctgc
     1441 cataataaaa ccaaaaatac cgggttttga gattgaatgc gcaacatcaa caaaaatgtg
     1501 atttgggtca acgtttggag aaagaagacc agactgaacc atagctttgc ctatccatcc
     1561 tgcatttgca acagcaaacg cagctaatgg cattaacaca aggcaaacca aaaacaaggc
     1621 tttcctgccg tcacgaggtg agcgtacact taaaaatcgc attaaaattc cctgatttat
     1681 aaaataaaat gccattgaac taccaaatgc gtcctgccag aaaatgccaa caaagttgaa
     1741 atcaggaggt tggttaaaag gagagaaggg caatttaaat tcataagaaa gtgatgtcca
     1801 gaatgtcttc aaactgccaa ttttatctat accaagaata aatattaaaa ttcctgctgt
     1861 caatagcact aaggcttgag ctaaatcggt cattattact gaagtttgcc ctcccgcatg
     1921 catatataaa gcacatacaa aagcaataac tgcggcagtt gccattacat cccagcctaa
     1981 catagcatta aaggcaacac caattgtata taaatttatt ccaatatagc ctaataagta
     2041 aattaacaaa attattacac taattgtgcg agtttttgag tcaaaacgtt tttcaaaata
     2101 ttcaggaatg gatgtaatgc gtccgtaata aataatgggt aaccagataa atacaaaaat
     2161 cggcagtata aaccagtcat tgagatacgt catggttgat gaaaatccat attcaaatgc
     2221 aacagcggaa tatttaatga aactgtatga accaacagtg gttgcaacac agctaaagcc
     2281 gattaaccac cagctaaagc gctgcccgcc aaaaaagaaa tcctttgtgc ttttgacata
     2341 tttaccaaaa aatgaaccaa agccaaggat aacaataaag tatattacaa ttattgcaaa
     2401 atcgatggtt gttcctttaa tcataactaa gctcctgcaa ttagtatcat caaagcatga
     2461 gcaaaaataa atataagata accgctaata atttctaata aaatttttct gtcagttggg
     2521 tggtgaatag aaatggcttt tttcttaatg gatagtacaa tcatagcaat aaaaaaaatt
     2581 gagcaaatag tatattggta aatgaatggt aaccatggtg acatcatgtg atcaactcct
     2641 catcaatggt gaaacgtgaa ggcaatagtc ttaattctgc attattgaaa tgagatgctg
     2701 ttttaaattt attaataggt aaacctatag atacggtata aagtatctgt aacgggtcgc
     2761 catgcgaaac aattaaaata gtatcattta tatgattggt ttcgcagtcc aacaaaaaag
     2821 aaatcatcct ttttgctaca gtatgtatag gttcaacgtc ataacatact ggatcattta
     2881 cattgcggca atccatatcc catattcgtt gatagttttc actgtctgtt ccctcaaatt
     2941 ggccaaaata acgttcgcgc aaacgtatat caagtttgac attgatgata ttcatatgcc
     3001 gggcaataat catggcagtt tcatatgcac gcaaaaaatc tgatgtatag atgtattcaa
     3061 ctgatttcag ttcattgcaa tgcgctattg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nacctgtgca actatttcta tagatgaatg tccccatcct atcctgtttt
     3241 tatattcatc gggtgtatat tgacaatcag tgacaagtat atcagcattt ttaatcagtg
     3301 tgccaatatt ttttaaatta tattcaccca attcgggatc gtttaaaact tcattatcac
     3361 tggcatatac aaacactctg tcatcatact caaccctgaa tcctaacgat aatcccggat
     3421 ggaaaagatg ggtatagtat ataacgatat cgttaatagt aaatgcattt tgggaaagct
     3481 ctataaaatt aatttttgat gataactcaa ataatgacac agggaaatat tctttgtcca
     3541 tttgtcctaa aatgagcttt tcaagtgttg agacactatg accaccataa aaattgattg
     3601 ttgcattttt gttatatgcc agtgcaaaaa aaggaaaccc ctgtatgtga tcccagtgtg
     3661 tatgactgat aaataaatga acctggatgg gtttatcaaa aaattcttta gcaaggcttt
     3721 ctcccagcat tctgatgccg gtaccggcgt caaatatcat gatagtattc ccacatctca
     3781 gctcaacgca ggcagtattg ccgccatatt taatgaaatt tattcccggt gtaggaactg
     3841 aaccacgtac tccccagaat cgcacaaaca ttgacaggtc tttttttttc atggtttatt
     3901 aaatttaaat tgatgcaaat agtatcatat tatataactc atcgacaaat aaaagaaaat
     3961 acttcaaaca tgaattaata cattgagcaa aaatatattc tattggctaa aactaatata
     4021 tgcagtattt gctattgaat aggttgcctt ttgatttttc aattcaacag ttatgtgtat
     4081 aacaaacggt aatgctaccc gtttggtaca gagtactttc atgggtgtat cattatagat
     4141 gtgttccagc aggctgccga taagtgaaaa tccatagatg gttgtattga tcttttcaag
     4201 gcgttctgct ttataagttt ttctggttgc aggattttca aaatatgcag taacaatcgc
     4261 atcgtttata ttggtagtac actcaacggc aaagcatagt gtatcgccct gaaaatagat
     4321 ataattccag atgtctttag cggttgaaca ccacgtacaa ggtgtaaagg tataatgcag
     4381 agaggcgttg gtgatagttc cgtttaatgc aacattgctt cctatattag ccttcattat
     4441 tgaaagtgtt tgatttgtgc atgctatgct caacacaagt gcgataatgg gtaatttact
     4501 gcggtgcaat aacaaattgc caccatcctt tca
//
LOCUS       DAOOJH010000144         4515 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15070_length_4515_cov_8.604484, whole genome shotgun sequence.
ACCESSION   DAOOJH010000144 DAOOJH010000000
VERSION     DAOOJH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15070_length_4515_cov_8.604484"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLJ75_11895"
     CDS             <1..243
                     /locus_tag="PLJ75_11895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD05957.1"
                     /translation="VKSRGNEIALMLTDLVMPGMNGVELYEAINKINPHIKNIFMSGY
                     TDLVIYEHIVNKESNYLQKPFSMFDLAKKLKHVLTS"
     gene            complement(305..1621)
                     /locus_tag="PLJ75_11900"
     CDS             complement(305..1621)
                     /locus_tag="PLJ75_11900"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05958.1"
                     /translation="MKTKHLIIFLSAIALITSCSSSAKKTDTKTDNTMNGLVKIYWED
                     KSLKGQGNVINGVKTGRWILYHKGSGEKLAEGEYVNDKQQGFWTYYHKNGAKSLEGNF
                     LEDQKTGEWLAYYPDGNIMWKATYVIKEKTVSGFTIKAGTIEGIKIWYYQSGKIWKEE
                     QYTNGEQTGRTQEYYENGTPKIIAWYKAGQLNGQCNKWYENGKKQEEGMYTENKKNGN
                     FKFYFDNGQVATTGSFVMDKPEGDWKFYSKEGLLQKEGKYNNGKEIGLWNFYMYPSRV
                     QRVKIMELPLQGGMIDNTNEKAVLYNEKGQIVGTGQLTGIPKGIYSIIKDGKQTGEIS
                     SSSVPVDNPKEKISYTWTGKWETPIKNGKWTEYYPGTKAIKLEAIYLMDKLNGAYKEY
                     YQNGKIKSEGEYLNGKMNGEWKFYNPDGSIDEAQSGRYMLGKKINF"
     gene            1964..2758
                     /gene="flgG"
                     /locus_tag="PLJ75_11905"
     CDS             1964..2758
                     /gene="flgG"
                     /locus_tag="PLJ75_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HPD05959.1"
                     /translation="MMRSLWTAASGMIGQQFNIDTISNNLSNVNTAGFKKMRAEFEDL
                     IYQTVLMAGTPATEISEVPTGIQVGHGVKISATQKLFEQGSLQSTGNKMDIALEGEGF
                     FKVQLYDGTIAYTRDGSFKVDSNRQIVTSNGYLLEPPIVLPENFIMETLSISQDGRVT
                     VKVVGDDDPIEVGQLEIARFVNPAGLQNIGGNLYKTTPASGEEIAGPPGIDGMALTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYEINSKAIQTSDSMLATAIGLKR"
     gene            2755..3294
                     /locus_tag="PLJ75_11910"
     CDS             2755..3294
                     /locus_tag="PLJ75_11910"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HPD05960.1"
                     /translation="MTYRFFIGVISICFIFVYSNAFADVVLYLKSNVIANDKITIGDI
                     ALVDGDEDIKSIENIELPESSKKDGYFTKDEVFQCIKNYVKGLTIIHGYSVKLITADG
                     SFSNSQDSYNELSPMMKRRTVSKGDFVMVKLIKKSIAIELMGRSMQSGTTGDEITVTL
                     QNGKVVTGTIHDDIIECKL"
     gene            3291..3914
                     /locus_tag="PLJ75_11915"
     CDS             3291..3914
                     /locus_tag="PLJ75_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HPD05961.1"
                     /translation="MKKIFVAIALVIASSVYADTIWKDKNLYTAQDAIKVGDIFTIIV
                     TDISQMRFTLTLANNDAFTVTSAPDGTITPFLPQINASRHATKNNTTNLQHRGNIAIS
                     IAATVQERLANGNFRVNGTKTFSFSGVTTIFNVSGIVNPASIKGGTINSSDIANLVLQ
                     IQGVKESVRITRAPLKEGESASYTLTEQEKQQIIIQYLQRILGELTQ"
     gene            3920..>4515
                     /locus_tag="PLJ75_11920"
     CDS             3920..>4515
                     /locus_tag="PLJ75_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HPD05962.1"
                     /translation="MKMVNKIIIACNLILFFHVSINAQVAVKIKDLIAIDGLKENQIY
                     GYGLVIGLQGTGDTKVALTRSSLINVLKNLGLSNENINSKNIAAVLITATLPPYVRVG
                     DRIDVIVSSIGDAKSLEGGVLLQSSLKGADDVTYVVAQGMLSITGDKRKGATIGKIVS
                     GGIVEREILPEVVVNGTIKFSLLNFDFSVAEEIKSAIE"
BASE COUNT         1418 a          784 c          787 g         1526 t
ORIGIN      
        1 gtaaaatcaa gaggtaatga gattgcatta atgcttaccg atcttgtaat gcccggcatg
       61 aatggagtcg agctctatga agctataaat aaaataaacc cgcatattaa aaatatattc
      121 atgtcagggt ataccgattt agtaatttac gaacatattg taaataagga aagcaattat
      181 ctacagaagc cattttctat gtttgattta gctaaaaagt taaaacatgt tcttacatca
      241 taattactca gatagacatt gctgctataa ttattaaata aggcagcaat gtctataatt
      301 tatttcagaa atttattttc tttcctaaca tatatcttcc cgattgcgct tcatctatag
      361 agccatctgg attataaaat ttccattcac cattcatttt cccatttaaa tattcgccct
      421 cggattttat cttcccattt tgataatatt ctttatatgc accatttaat ttatccatga
      481 gatatatagc ttcaagtttt attgcttttg ttccaggata atattctgtc cattttccat
      541 tttttattgg tgtttcccat ttacctgtcc atgtatagct tatcttttcc ttaggattat
      601 caactgggac tgacgatgat gaaatttcac ctgtttgttt tccatctttt atgatactgt
      661 aaattccttt tggaataccg gtaagttgtc ctgtgccaac gatctgacct ttttcattat
      721 acagcacagc tttttcattt gtattgtcaa tcattccacc ctgaaggggg agttccatta
      781 tctttactcg ctgaacacgt gaaggataca tataaaaatt ccacaatcca atctctttgc
      841 cattgttata tttcccttct ttttgtaata gcccttcttt agaataaaat ttccaatcac
      901 cctccggttt atccatcaca aaacttcctg tcgtagcaac ctgtccatta tcaaagtaaa
      961 atttgaagtt gccgttcttc ttattttctg tatacattcc ttcttcttgt tttttgccat
     1021 tttcatacca tttattacat tgtccgttca attgacctgc tttataccat gctataattt
     1081 ttggtgttcc attttcatag tattcctgag tccttcctgt ttgctcgcca ttggtatatt
     1141 gttcttcttt ccatattttt cctgattgat aataccaaat ttttatccct tcaatggtgc
     1201 cagcttttat ggtaaatccg ctcactgttt tttcttttat tacataggtg gctttccaca
     1261 taatgttgcc gtcaggataa taggctaacc attcccctgt cttttggtcc tctaaaaaat
     1321 taccttctaa actttttgct ccgtttttgt gatagtatgt ccagaaaccc tgctgtttat
     1381 cattgacata ttcgccttca gctaattttt ctccgctacc tttatggtac aatatccatc
     1441 ttcctgtttt aactccatta ataacattcc cctgaccctt caatgatttg tcttcccaat
     1501 agattttaac aagaccattc atagtattat ctgtttttgt atcagtcttt tttgctgaac
     1561 tgctacatga tgttatcaag gcaatcgctg aaagaaatat aatcaaatgt tttgttttca
     1621 taaaaaaatc tccttcgtct attgttatac accttttaat taaaattctc taaatcactt
     1681 ttttcatagt actgacaatt gaaaaaagcg gataaaacaa catctgatgc ctacacaatt
     1741 actaaaaatg taatttctgg atgttattat atagtaagta catagtaaac gttagaaagt
     1801 tacaataatc aaaacaatgt tgtaggcgat agtttcttat tattattttt tgcttttaat
     1861 ctatatttat tatagtaaat cattttgtca atcaattatg tcaagcaaat aaataattat
     1921 gccgaaaaat atatataaga ttatatataa acgaggtgac aatatgatgc gttcattatg
     1981 gactgcagca tctggtatga taggacagca atttaacata gataccattt caaataattt
     2041 atcaaatgta aatactgcag gatttaaaaa gatgcgagcc gaatttgagg atttaatcta
     2101 ccagactgtg cttatggcgg gaacacctgc aactgaaata agtgaagttc ccacaggaat
     2161 tcaggtcggc catggagtaa agatatcagc tacacaaaaa ctgtttgaac agggaagttt
     2221 gcaatcaacc ggtaacaaaa tggatatagc actggagggc gaaggatttt ttaaagtaca
     2281 attatatgat ggtactatcg cctatacccg cgatggttct tttaaagtag attccaacag
     2341 gcagatagtt acttcaaatg gctatctatt agagcctcca attgtactac ctgaaaattt
     2401 tattatggaa acattgtcta tcagtcagga tggaagggta acggttaagg tagtagggga
     2461 tgatgaccca atagaagttg gacagcttga gattgcacgt tttgtaaatc ctgccggatt
     2521 gcaaaatatt ggtggcaatt tatataaaac aacaccagcc tcaggcgaag agattgcagg
     2581 acctcccgga attgacggaa tggcacttac tcatcagggc tttttagaaa tgtcaaatgt
     2641 taaggttgta gaagagatgg tcaatatgat tgttgcccaa cgtgcatacg agataaactc
     2701 aaaagctata cagacgtctg attcaatgct tgccactgca attggattaa aacgatgacg
     2761 tatcgatttt tcattggtgt cattagcatc tgttttattt ttgtttattc gaatgctttt
     2821 gccgatgttg tgctgtattt aaaatcgaac gtcatagcca atgataaaat caccattggc
     2881 gatatagctc ttgtagacgg tgatgaggac atcaagagca ttgaaaatat tgaattgccg
     2941 gaatcgtcta aaaaagatgg ttattttacc aaagatgaag ttttccagtg tataaaaaat
     3001 tatgtaaaag ggcttactat tatccatggc tactctgtta aactgataac cgcagatggt
     3061 tcattttcaa acagtcaaga ttcatacaat gaactatcgc ccatgatgaa acgtcgtacc
     3121 gtaagcaaag gtgattttgt catggtaaaa cttattaaga aatcaattgc tattgagttg
     3181 atgggcaggt cgatgcaaag tggtacaaca ggcgatgaaa ttacagtgac actacaaaat
     3241 ggcaaggttg tcaccggaac aattcatgac gatattattg agtgtaagct atgaaaaaaa
     3301 tatttgtggc gatagctctg gttattgcat cttcagtata tgctgataca atctggaaag
     3361 ataaaaacct ttatacagca caggatgcta ttaaagtagg tgatatattt actattattg
     3421 ttacggatat atcgcagatg cgatttactt tgacacttgc caataacgat gcttttacgg
     3481 ttacttctgc gcctgatggc accataacac cttttttacc tcagataaat gccagcagac
     3541 atgctaccaa aaataatacc acaaatttgc agcacagggg aaatatagca atctcaatag
     3601 ctgcaacagt acaggaacgt ctggcaaatg gtaattttag agtaaacggc acaaaaacat
     3661 tttcatttag tggcgtaaca accattttta atgtctcagg aatagttaat cctgcttcta
     3721 taaaaggtgg tacaattaat tccagtgaca tcgctaattt agtactgcag atacagggtg
     3781 ttaaagagag tgtacgtata acacgagcac cattgaagga aggcgaatcg gcttcatata
     3841 cattaaccga acaggaaaaa cagcagatta taattcaata cttacaaaga atattgggcg
     3901 aattaacaca ataatagcta tgaaaatggt aaacaaaata attattgcat gtaacctgat
     3961 tttatttttt catgtgagta taaatgcaca ggttgctgta aagattaaag atttaattgc
     4021 aattgatgga ttaaaggaaa accagattta cggttatggg cttgttatag ggttgcaggg
     4081 taccggtgat accaaggttg cgttaacccg gagctcgctt attaatgttt taaaaaattt
     4141 agggctcagt aatgaaaata ttaattcaaa aaatattgct gcagtgctta ttactgcaac
     4201 attgccacca tatgtcaggg taggcgatcg tattgatgtt attgtgtcat ccattggtga
     4261 tgcaaaatca cttgaaggcg gagtgctgtt acaatcttca ctcaaaggag ctgatgatgt
     4321 tacgtatgtt gtagcacagg gcatgctatc tattacaggt gataaacgta aaggtgccac
     4381 tattggaaaa attgtttccg gtggtattgt tgaacgtgaa atattgcctg aggttgttgt
     4441 taatggaact attaagtttt cattattaaa ctttgatttt tcagttgcag aagaaatcaa
     4501 atcagcaatt gaaaa
//
LOCUS       DAOOJH010000145         4424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15446_length_4424_cov_8.691463, whole genome shotgun sequence.
ACCESSION   DAOOJH010000145 DAOOJH010000000
VERSION     DAOOJH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15446_length_4424_cov_8.691463"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..468)
                     /locus_tag="PLJ75_11925"
     CDS             complement(310..468)
                     /locus_tag="PLJ75_11925"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPD05963.1"
                     /translation="MEHQKGSLSKGKVADMAVLSNDILSISPDKIKDVKVIMTIVGGM
                     IVYRNDVL"
     gene            complement(473..1015)
                     /locus_tag="PLJ75_11930"
     CDS             complement(473..1015)
                     /locus_tag="PLJ75_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05964.1"
                     /translation="MIILYCHAGFVEASITFLFCTLNRAYVEKESDTILLLYNGNIIT
                     MGNSITKPEAIVVKGSRILFLWKLQEALKKAGDTAIKINLKGKTLIPVFNDNHTHTLA
                     AGSFYSEIILWNKTCEDIALIINEEAKKKKPGELITGNSWEPFTVRLLKKYRDSQELS
                     CRFTCWPLGDSPLYYGLGIC"
     gene            complement(1091..1927)
                     /locus_tag="PLJ75_11935"
     CDS             complement(1091..1927)
                     /locus_tag="PLJ75_11935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPD05965.1"
                     /translation="MKRYNDFEDLSINQYFNAINQVELLTQEEEIDLAKKKGIGDEKA
                     KESLINANLRLVVKIAKNYTNLESSLIDLIQEGNLGLIRAAEKFDYNMGCRFSTYASY
                     WIKHYITRFIAKRSRTIRVPIRKGDLFKKIKRVQDELTNELGKEPSYRDIADMLGIDE
                     RSVSDILEVFQPTVSLEYPLNDDEFNLYEVVSDEKFLSPDTSISRRDLKKELKSAMES
                     LMENERLILTKRFGLDGSEPVTLKDVGNEFGISAETVRQIEARAMQKIKQRFSYLADY
                     IE"
     assembly_gap    2002..2101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2302..2502)
                     /gene="rpmE"
                     /locus_tag="PLJ75_11940"
     CDS             complement(2302..2502)
                     /gene="rpmE"
                     /locus_tag="PLJ75_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPD05966.1"
                     /translation="MKKGIHPEYFDTVIKCACGNEIHTRSTVKDLHVEICSNCHPFYT
                     KKQKIIDTTGRVEKFKKKYNIK"
     gene            complement(2539..3894)
                     /gene="rho"
                     /locus_tag="PLJ75_11945"
     CDS             complement(2539..3894)
                     /gene="rho"
                     /locus_tag="PLJ75_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPD05967.1"
                     /translation="MTKVSNETTEKNNNNRKNGNGDYSRKNNNGTIQEGRLDLAELKS
                     KTISALMEIARSMGIEGISGLKKQDLIFEILKAQTERSGLIFSSGVIEVLNDGYGFLR
                     SPNYSYLPGPDDIYVSPSQIRLFGLRTGDTVAGQIRPPKDNERFFALLRIEAVNYENP
                     EKLQNRVLFDNLTPLYPETRINLETTPEEIDMRILNIIAPIGMGQRALIVAPPRTGKT
                     IILQKIANAITTNNPDIILIVLLIDERPEEVTDMERSVKGEVISSTFDEPASRHVQVS
                     EMVLEKAKRLVEHKKDVVILLDSITRLARAYNQVVPTSGKILSGGVDSNALHKPKRFF
                     GAARNIEGGGSLTIIATALIETGSRMDEVIFEEFKGTGNCEIVLDRKLADRRIFPAID
                     INKSGTRKEELLVDAEELNKIWILRKVLSSMSSTESMEFLIEKMRSTKNNRAFFKAMQ
                     G"
BASE COUNT         1340 a          819 c          676 g         1489 t
ORIGIN      
        1 tattgtttta aaggaggtcc ccaaacctcc tttttatttt tatagagata atctgcggac
       61 tatttaatgt gagatgatga agcatcagga tgctggtatt gatacatttt catagtaccg
      121 taaaaaaaag aattttttag ttaaaaacta ttccttttaa ggatattttt attcctccgt
      181 ctattttaga gggtagcatg gtattatata atcaggaata aaaatgaaat acaaaattta
      241 ttaaatcttt aagcagaaat aattgccatc catttaaata ataatgtaat acaatatggt
      301 ttgtacagtt tataatacat cgtttctata tactatcatg ccaccaacta tcgtcataat
      361 aacctttacg tctttaattt tatcaggtga tatggataaa atgtcatttg aaagcactgc
      421 catatcagct accttaccct tgctcagtga acctttttgg tgttccataa attcagcata
      481 tgccgagccc atagtataaa ggtgaatcgc ccaaaggcca gcaggtaaat ctgcacgata
      541 gttcctgtga atcacggtat ttttttaata aacgtactgt aaatggttcc catgagttac
      601 cagttataag ctctcctggt tttttctttt tagcttcttc attgataatc agggcaatat
      661 cttcacaggt cttgttccat aaaattattt cagaataaaa ggagccggca gctaaggtat
      721 gggtatgatt atcattaaat acgggaataa gtgttttacc ttttagatta atctttattg
      781 cagtatcacc tgcttttttt aatgcttcct gtaatttcca caaaaacaat attctgctac
      841 ctttaacaac aattgcttcc ggtttagtaa tactattacc catagtaatg atattcccat
      901 tatacagtaa caatatcgta tcactttctt tttcaacata tgcacgattc aatgtacaaa
      961 acaggaatgt gatagatgct tcaacaaatc cagcatgaca ataaagaata atcatagttg
     1021 tttagaggag gagcttgatg ccggcaggga aaatataaaa gggcaggtta acaacctgcc
     1081 ctttaagaaa ttattcaata taatcagcta aatacgaaaa tcgctgctta attttctgca
     1141 ttgctcgggc ttctatctgg cgaactgttt cagcagaaat accaaactca tttccgacat
     1201 cctttaaagt aacaggttca ctcccatcaa gcccaaacct ctttgttaag ataagccgtt
     1261 cattctccat taatgattcc attgctgact ttagttcctt ttttaaatct ctgcgtgaaa
     1321 tgctggtgtc aggagaaaga aatttttcgt cgcttaccac ctcataaaga ttaaattcgt
     1381 cgtcattaag cggatattct aatgaaacgg taggttggaa tacctctaat atatcgctta
     1441 cacttcgttc atcaataccc agcatatctg caatatctct ataagaaggt tctttcccta
     1501 attcgttggt aagctcatcc tgaacccgtt taatcttttt aaagagatcg cccttacgga
     1561 taggaacacg gatagtgcgg cttcgttttg caataaaacg tgtaatatag tgcttgatcc
     1621 agtatgaagc atatgttgaa aaacggcagc ccatgttgta atcaaacttc tctgctgctc
     1681 ttattaaacc taaattgccc tcctgaatca ggtcaataag agatgactct aaatttgtat
     1741 aattttttgc aatctttaca accagccgca aattagcatt aatcaacgat tcctttgctt
     1801 tttcatcacc gatgcctttt ttctttgcta aatcaatctc ttcttcctga gtgagaagtt
     1861 caacctgatt aatggcattg aaatattgat tgatagataa atcttcaaaa tcgttatatc
     1921 ttttcatggc tgcctcctat ccatgatttc ctcatcacta tattttaaag caatttatgt
     1981 gccaaatact tcattttttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 ncaattgtgc ctaaaaacaa agaataaagt caacaagtgt ataataaata cacatagaca
     2161 tagtttagtg tgtattaatt acacataata gaacattttt gccaatatac cattaataaa
     2221 taaacgcact cactacaagc gcgttaattg ttataactgc atggtatcta tccgaaggat
     2281 agaatatccc attcctgtag attatttaat gttatatttc ttcttaaatt tttctacacg
     2341 tcctgttgtg tcaataatct tttgcttctt ggtataaaac ggatgacagt ttgaacatat
     2401 ttcaacgtgc agatccttaa ccgttgaacg agtgtgaatc tcattcccac atgcacactt
     2461 tatcacagta tcaaaatatt caggatgtat accttttttc ataataacta tctccttatg
     2521 ccaaataatt atatcgcact atccctgcat tgctttaaaa aatgctctat tattttttgt
     2581 actgcgcatt ttctcaatta aaaattccat ggattccgtt gaactcatag acgataatac
     2641 ttttcgcaaa atccatattt tattaagttc ttctgcatct acaagcaact cttccttacg
     2701 ggttccggac ttgttaatat cgattgcggg gaatattctt cgatcagcta attttctgtc
     2761 aagcacgatt tcacagtttc cagtaccttt aaattcctca aatattactt cgtccatacg
     2821 gctgccagtt tctataagtg ctgttgctat gatggttaag ctcccaccgc cttctatatt
     2881 acgggctgca ccaaaaaaac gttttggttt atgcagggca ttggaatcaa ccccaccgga
     2941 aagtatcttt ccgcttgttg gcaccacctg gttgtatgct cgtgctaaac gggtaatcga
     3001 atcaagtagt ataacaacat cttttttatg ctcaacaagg cgctttgcct tttcaagtac
     3061 catctcacta acctgaacat ggcgtgatgc aggttcgtca aacgttgatg aaataacttc
     3121 acctttgacc gaacgttcca tatctgtaac ctcttcagga cgctcatcaa tgagcaatac
     3181 aatcaagata atatcgggat tgtttgtggt aatagcattt gcaatttttt gtaagataat
     3241 ggttttgccg gtacgcgggg gtgcaacgat aagtgcacgt tgacccatgc caataggtgc
     3301 tatgatgttc agaatacgca tatcaatctc ttcaggtgtt gtttcaaggt taatccgtgt
     3361 ttcgggatat aatggcgtaa ggttatcaaa aaggaccctg ttctgcaatt tttcaggatt
     3421 ttcgtaattg acggcttcta tccttaaaag ggcaaaaaag cgttcattat ctttcggtgg
     3481 gcgaatctgc cctgcaacag tatcaccagt tcgtaatcca aaaagccgta tctgagaagg
     3541 ggaaacatat atatcgtctg gtcctggaag gtaactatag tttggcgatc ttagaaaacc
     3601 atacccatca ttaagaactt cgataacacc gctggaaaat attaatccgc tccgctcagt
     3661 ttgagctttt aaaatttcaa aaatcaaatc ctgctttttt agtccggaaa ttccttcaat
     3721 acccattgat cgggcaattt ccatgagagc acttattgtt tttgatttaa gttcagctaa
     3781 gtctaaacga ccttcttgaa tagttccatt attatttttt cttgaataat ctccgtttcc
     3841 gttttttcgg ttattattat tcttttcagt agtttcattg gacacttttg tcataaatca
     3901 ccttttgata cattatactt tataatagtc aactgtcctt tatgttattg agaaacaagg
     3961 gcatatttgc ttacttggtg tgtaattgtt acactaatag cgttataaaa aaaactcatg
     4021 gcagtgtgtg aaatcgtaat tagttaataa aatactttga acacttctaa aaactgtttt
     4081 tcgtttgatg acaaacaatt aaaacaattg ggaaaagcag ttttacgaat atgttattac
     4141 cacacgggta ttaaagaatt aaagatttag atatcccctt atacctgtaa taaagcattt
     4201 catattgtgt tgtttttact atgttattaa atattaacgt catgataaaa tatatattta
     4261 gctttttaat aatccactgt atccgggcag gataataatg ttgaatgaac tattctccct
     4321 taaatatact ataaaaaaac aaatatgcca caaaaaaata aaatattttt ttatttttta
     4381 ccaccctata ccataataca atttcgtcaa gaaaaaatgt aaaa
//
LOCUS       DAOOJH010000146         4401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15537_length_4401_cov_6.055223, whole genome shotgun sequence.
ACCESSION   DAOOJH010000146 DAOOJH010000000
VERSION     DAOOJH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15537_length_4401_cov_6.055223"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PLJ75_11950"
     CDS             <1..554
                     /locus_tag="PLJ75_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPD05968.1"
                     /translation="IKNSKPYIIISNHQSQLDILVAFRLYKHFKWVSKAEIFKVPVIG
                     WNMVLNRYIKLVRGDKKSIAQMMDECEMHLKEGSSVYIFPEGSRSPDGHLKQFKLGAF
                     LLAKKLNLPIMPIIIEGTRFALPKHSIDYHGKHPIILRVLDEIVPEIFKNMSIEELRD
                     YTWNYMHTELKKLRMEMYGSDSI"
     gene            661..1506
                     /locus_tag="PLJ75_11955"
     CDS             661..1506
                     /locus_tag="PLJ75_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05969.1"
                     /translation="MSQPDDFSLDDDLFRFDDEIDIQDNIPENKPTANNNRVMDSGKD
                     FGDSSRKRSSRDFQPDMDALLITAQSSMIIEGMRKYTERDFSSSNLPIYLEALNGVNL
                     YIKIIDRNPKNYHKLKSVINADQDCQEVEKIAFDLYKKMFGTVPETDQEKLVAFEKFS
                     SLFKDAVNRATINASMKEIKKYFLLSGGLDEIKVSKLIAQNDEELAKDISNLQRYIAM
                     GLAYIEKGNIEIIKGLKGRDLNNYIIRGSQLLTFYFKKINNDRLADYYSRINSIYSKY
                     FVIKD"
     gene            complement(1653..2804)
                     /gene="hemW"
                     /locus_tag="PLJ75_11960"
     CDS             complement(1653..2804)
                     /gene="hemW"
                     /locus_tag="PLJ75_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPD05970.1"
                     /translation="MIHSGIYIHIPFCNTKKCDYCDFYSIPCNPSHSNFQQIKHAYIN
                     ALVSEIIHYSSELNNYLVDTIYIGGGTPSLLQCSDYNVLLTVLHDYYAIASEVEITLE
                     CNPDDYSKNYIQQLSHIGFNRFILGVQTLDTRAHKYIGRKSMLCTMEEIQEFCTIPDI
                     IPCIDIIAGIPVQSVTNYRSMLNSIIQCNPKHISAYMLTYETNTPLRLRTKNPLQYAN
                     FQRIMFQKTIDALVASGYNHYEISNFSIPGYESKHNLKYWNFESYIGFGSGAHSFYNN
                     KRYYNGTIDDYMNDAIASRTEDIRTQPDSVLEYCMNKLRLKQGFMINDMRNKTGYLPA
                     ESFYQSIHRLIDKSLLIMQTCDGDICIQCTDEGMFMLDTILLELASSVS"
     gene            complement(2801..>4401)
                     /gene="nagZ"
                     /locus_tag="PLJ75_11965"
     CDS             complement(2801..>4401)
                     /gene="nagZ"
                     /locus_tag="PLJ75_11965"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HPD05971.1"
                     /translation="TAVSLLLVLVQLIGCKQPKDYITAGKIASGLANDVLLGQMLIIA
                     LPSGVVDDKTIAIIKKYKPGGVIIFGYNLTDGSCKTFCNDLQNIAMEHIGIPLFIAID
                     QEGGRVKRIQNGVTQFPGNFALGVINNPSKVYRMAQIVGMQLRIAGINMNFAPVLDVN
                     NNFNNPVINIRSFSCIPQVCANLGAAYIQGLQQSRCIAVAKHFPGHGDTYQDSHITLP
                     VINKTHASLNKTEFVPFKKAISSGVECIMTAHIAFPQLGIVKPATMSEYFLNHLLRKK
                     MKFDGLIITDDLEMKGISGYYSQGTAAVASVKAGADIVLISSYEDHVQDIINSLSNAM
                     VKNEVSKDNIVSTVKKIIEVKMRYGIMTIKKNKIYNSNFNGGDSYLEMMQQADTLNTE
                     LSRESIVYHSKNDDFTTFIPAENRKATILTTDKEVEDIFKAINADVIHSIQLLKNKKI
                     LFIDSGCYTTKQLQSIIKTAKQNDCNTLLLVTGNPYPYLKEFGEYPLLMSFSDTHESL
                     HQMALCLSGEFTPKSSHPCLMIDK"
BASE COUNT         1439 a          731 c          793 g         1438 t
ORIGIN      
        1 agatcaaaaa tagtaaaccg tatatcatta tttccaatca tcagtcacag cttgatatac
       61 tggttgcatt cagattatat aaacatttca aatgggtgtc aaaggcagaa atatttaagg
      121 tgccggttat aggctggaat atggtattga accgatatat caaactggta cgaggcgata
      181 aaaaaagtat agcacagatg atggatgaat gtgaaatgca tttaaaagaa ggtagttctg
      241 tgtatatatt tccggaaggc tcacgttcgc ccgatggcca tttaaaacaa tttaagttgg
      301 gagcatttct attagcaaaa aagttgaatc ttcccataat gcccattatc atagaaggta
      361 cacgatttgc tttaccaaaa cacagcatag actatcatgg taagcatccc ataatattac
      421 gggtacttga tgaaatagta ccggaaatat ttaaaaacat gagtatagaa gaactgagag
      481 attatacatg gaactacatg catactgagt tgaaaaaatt aagaatggag atgtatggta
      541 gtgattcaat ctgagaataa ttatacctta aattagcttg cactattgat gtatattctg
      601 tagagtaata atatatacca gattgtgaaa tttaaactat tttacagaga ggttgcatta
      661 atgtcccaac ctgatgattt ttccttagat gacgatttat ttagatttga tgatgagata
      721 gatatacagg acaatatacc cgagaataaa cctaccgcaa ataacaatag ggtaatggat
      781 tcgggaaaag attttggcga tagctcccgt aaacggtcat ctagagattt tcagcccgat
      841 atggatgcac tgctcattac agcacagtct tcaatgatta ttgaaggaat gagaaaatat
      901 actgaacgag atttttcttc aagtaatctc cccatttatt tagaagcatt aaacggtgtt
      961 aatctttata taaaaataat agatcgtaat ccaaaaaatt atcataaatt gaaatctgtt
     1021 ataaatgctg atcaggattg tcaggaagtt gaaaagattg catttgatct atacaaaaaa
     1081 atgtttggta ctgtgcctga aacggatcag gaaaaactgg tggcatttga aaagttttca
     1141 tcattattta aggatgcagt caatagagca actatcaatg cttccatgaa agaaataaaa
     1201 aaatactttt tattgtcagg ggggcttgat gaaatcaaag tttcaaagct tatagcccaa
     1261 aacgatgaag agcttgcaaa agatatttca aatttacagc gatatattgc catgggattg
     1321 gcctatattg aaaaagggaa tattgaaata attaaaggat taaaaggaag agatctaaac
     1381 aattatatta tacgtggttc ccaactgttg acattttact ttaaaaaaat caataatgac
     1441 cgccttgcgg attactattc gaggatcaat tctatttata gcaaatattt tgttataaaa
     1501 gattaatact cccatctttt ttacttgata tagtgtaaat aatattaagc ttagataagc
     1561 agaacaacct ttttttagtg cattgatttg ccgacacata ttactatggc aatacatatt
     1621 tttaattttt tcaatcaatc atcattcaat tgctatgata cactgcttgc taattcaagc
     1681 agtattgtat ccagcatgaa catcccctca tccgtgcatt gaatgcagat atcaccatca
     1741 caggtttgca tgattaataa tgatttatca attaaacgat gtattgactg gtaaaatgat
     1801 tcagccggca ggtaccctgt tttgttacgc atgtcattga tcataaatcc ctgttttaac
     1861 ctgagcttat tcatacaata ttcaaggacg ctgtccggtt gtgtgcgtat atcctccgta
     1921 cggcttgcaa ttgcatcgtt catgtagtca tcaatggtac cattgtaata gcgtttgttg
     1981 ttataaaatg aatgtgcacc gctaccaaaa ccaatatagc tttcaaaatt ccagtatttt
     2041 agattatgtt ttgactcgta tcccgggatt gaaaaattgg aaatttcata atggttgtag
     2101 cctgatgcca ccagcgcatc gattgttttt tgaaacatga ttcgctgaaa atttgcatat
     2161 tgcaggggat tttttgtgcg taagcgcaaa ggtgtatttg tttcatacgt cagcatatat
     2221 gcagagatat gtttgggatt acactgaatg atactgttga gcattgaacg atagttagtt
     2281 accgactgaa cgggaatgcc ggcaataata tcaatgcatg gtataatgtc aggaatagtg
     2341 caaaattcct gtatttcttc catggtgcaa agcattgact ttcttccaat atacttatgt
     2401 gctctggtat caagtgtttg tactcccaat ataaatctat taaagcctat gtgcgatagt
     2461 tgttgtatat aatttttgga atagtcatcg ggattacatt ccaaagtaat ctcaacctca
     2521 gaggcaattg cataataatc atgcagtaca gtaagcaaga cattgtaatc actgcattgc
     2581 agcagcgacg gagtaccccc tccaatatat atggtatcta ccagataatt gtttagttca
     2641 gatgaataat gaatgatttc ggataccagt gcattgatgt atgcatgttt gatttgttga
     2701 aaattgctat gtgatggatt gcacggtatg gaatagaaat cgcagtaatc acatttctta
     2761 gtattgcaga atggaatatg aatatatatg ccagaatgta tcatttatca atcattaaac
     2821 agggatgaga tgattttggt gtaaattcac cactcaggca aagcgccatc tggtgcaatg
     2881 attcatgagt atctgaaaaa gacattaaaa gaggatactc gccaaattct tttagatagg
     2941 gatagggatt gccagttacc aataaaagtg tattgcaatc gttttgtttt gcagttttaa
     3001 taattgattg caactgtttc gtagtataac atccactatc aataaaaaga atttttttat
     3061 ttttcaataa ctgtattgaa tgtattacat ctgcatttat agctttaaaa atatcctcaa
     3121 cttccttatc cgttgtaagg atagttgctt tcctgttttc ggctggtata aatgtagtga
     3181 aatcatcgtt ttttgagtga tacactattg actcccttga taattcagta ttcagtgtat
     3241 cagcctgttg catcatttcc aggtaactat cgcccccatt aaaattacta ttatatattt
     3301 tatttttttt gattgtcatg ataccataac gcattttaac ctctataatt ttttttactg
     3361 ttgatacgat gttgtctttt gatacttcat tttttaccat tgcatttgat aacgaattga
     3421 taatatcctg aacatgatct tcataggagc ttatcaacac aatatcagcc cctgctttaa
     3481 cgcttgcaac agcagcagtt ccctgtgaat aataaccgct gatacccttc atctctaaat
     3541 catcggtaat gattaaacca tcaaacttca ttttttttcg taataaatga tttaaaaaat
     3601 attcagacat ggtggcaggt tttacaatgc caagttgggg aaaagcaata tgagccgtca
     3661 taatacattc tactccggaa gatattgctt ttttgaaagg gacgaattca gttttattca
     3721 atgatgcatg agttttattt atgaccggca atgttatgtg cgaatcctga taggtatcac
     3781 catgccccgg aaaatgtttg gctacagcaa tacatcgtga ctgttgtaat ccttgaatgt
     3841 atgcagctcc aagattagca caaacttgag gtatgcagct aaaggaacgt atattgataa
     3901 caggattatt gaaattgtta ttgacatcaa gtaccggagc aaaattcata ttgatgcctg
     3961 ctattctaag ctgcatccct acaatctgtg ccatccggta cacttttgat ggattattaa
     4021 tgacaccaag tgcaaaatta ccgggaaact gcgttacgcc attttgaatg cgctttaccc
     4081 tgcccccttc ctggtcaatg gctataaaca aagggatacc aatatgttcc atagcaatat
     4141 tttgaagatc attgcagaat gttttgcagg agccatcagt aaggttataa ccaaagataa
     4201 taacgcctcc cggcttgtat ttttttatta tagctattgt cttatcatca acaacacctg
     4261 atggcaaagc aatgataagc atctgcccca gtaaaacatc attggctaat ccactggcaa
     4321 tttttcctgc tgtgatataa tcttttggtt gtttacatcc aattagttga actaaaacta
     4381 ataagagaga aacagcggtc a
//
LOCUS       DAOOJH010000147         4368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15674_length_4368_cov_10.373058, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000147 DAOOJH010000000
VERSION     DAOOJH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15674_length_4368_cov_10.373058"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PLJ75_11970"
     CDS             <1..1410
                     /locus_tag="PLJ75_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05972.1"
                     /translation="EVSNSMPDKTLIVIKSKIHCMKLNTSGLLFIVIDKENDWIIQVD
                     AIFISGDFLKNAKINIPSVDFFEAITVAREQYMIIKEYSDSVKNYILHKIDQMNIRTL
                     INNINNNQIAAINDIQRIVKSALNIDIVDVYSEFDTITNAQYNEIVVKQKIESLTLAK
                     QVELVAGYYGNDKKITEESIKKNKQDIGIAVVYITSKLFTGICFCFVNCITKKMLYCY
                     SWIDENDTIQKELNAKDTLYDLFQKVAETTQRINKLTPIQHEIQSKLWSINIDMLINA
                     IQHEASGIAGQLFHDVLQQYPDIKVRAELHTIDAIRFHMLFNPPQFDNNQEEQIGDIK
                     IDKKMVAVDLVLSPTSGKKVSQLQRGDYIYVLIDTSTPYGYDVAKALNLILDNKNTPI
                     EAEVYSVIYNSKKGFNIFVKISDSLYGKALEEHDVKVKIKLVADDESKKEVSSRTSLV
                     IGIIAGIVVIAVIAFLILS"
     gene            complement(1418..2683)
                     /locus_tag="PLJ75_11975"
     CDS             complement(1418..2683)
                     /locus_tag="PLJ75_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPD05973.1"
                     /translation="MKRIYIIIGIIAAVMLIFIFLIHSNNDKLKNDDPSKNQSLLNIF
                     QFMSQQEQSEQLPPVDPEKVETYEIEQANFIKDIILEKNPVCAGEDFKVTVIAKNPFG
                     SDAHLVYRISNKLGNPAIMRFNKAGQREFYVTVRDEGKHIDMRKVQITVVECPDKPLV
                     VLSARLHNLEPESAIFEVTSQKGLSGKCTYEWDFGDGTYATTQNGYITHNYASRDQKG
                     LQSSFLVKVTVADANNITATGRNSISFPNVHYLSNLMGNPIIPVLYNKFPSIDSSTIT
                     VEVTMKNIFDEAVSFTNAVLECMPCNTEMSPDYKNINPDQLLESTYIAPRSTVKQLLK
                     ISRYLLPKSTCNLTITLSGQYPGNREVYAKIYLNIPPQSKEDIDASRDKIITDDDMIE
                     KLNKASKILGKDKPITPQDIQKLEREGKL"
     gene            complement(2901..3893)
                     /locus_tag="PLJ75_11980"
     CDS             complement(2901..3893)
                     /locus_tag="PLJ75_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05974.1"
                     /translation="MKFISKICLIMLIVFTTSTIFAGQWVYKPMSINAQKGDVILSTS
                     PGFIMDLLAILGCYWSHSGMTVDNGFNIRHNTMYVSDIPIEYNKIWFIQTTPKRMNPD
                     KLSNGMPGILTEDIDTTYNVTKNFAAAGGAVLKPASANEALYRQYLVNAADKMLYVKG
                     YYRVNAYVNMYQLDYVNYYIKGRGNHCSGTCWYANYFAGKTMNVAHIPPSLVSQCGYS
                     LYNSVKNMVRDEAGGFGSFIIDIEGLFGTGADEKIANQIVNTFGWDRSTDTSDYWKSY
                     INKISATANAPDHLLLYTYTNPSGKNTGVQTTSSSYYGQVDPLVITSGYYYWVD"
BASE COUNT         1487 a          668 c          752 g         1461 t
ORIGIN      
        1 gaggtaagca attctatgcc agataaaacg cttattgtta ttaaaagtaa aatacattgt
       61 atgaaactga atacatcagg attacttttt attgttattg ataaagaaaa tgattggatt
      121 attcaagttg atgcaatttt tatttcgggt gattttttaa aaaatgcaaa aataaatatt
      181 ccatctgttg atttttttga agcaataact gtggcacgcg aacaatatat gatcatcaaa
      241 gagtattcag atagtgtaaa aaattatata ttgcacaaaa ttgatcaaat gaatatacga
      301 acacttatta ataatattaa taataatcaa atagccgcaa ttaacgatat tcagcgaatt
      361 gttaaatctg cattaaatat tgatattgtt gatgtgtatt cagagtttga tactataacc
      421 aatgctcaat ataatgaaat agtggtaaaa caaaaaatag aaagcttaac attagcaaag
      481 caggttgaat tagtagctgg atattatggc aatgataaaa aaataacaga agaatcaata
      541 aaaaagaata aacaggatat aggtattgct gttgtatata ttacttcaaa gctatttaca
      601 ggaatatgct tttgttttgt taactgcata acaaaaaaaa tgctctactg ttatagctgg
      661 attgatgaaa acgatactat tcaaaaagaa ttgaatgcaa aggatacgtt gtatgattta
      721 tttcaaaaag ttgctgaaac aacacaaagg ataaataaat taacaccgat ccaacatgaa
      781 atacagtcaa aactgtggag tattaatata gatatgctga taaatgcaat tcagcatgaa
      841 gcgtcaggga tagcaggtca actttttcat gatgttttgc agcagtatcc tgatatcaag
      901 gtaagagctg aattgcatac tatagatgct attcgctttc atatgctatt taatccgcca
      961 caatttgata ataatcagga agaacagatt ggtgatataa aaattgataa aaaaatggta
     1021 gcggttgatt tggttttatc gccaacatca ggtaaaaaag tttcacaatt acaacgtggt
     1081 gattacatct atgtgcttat tgatacttct acgccgtatg gatatgatgt ggcaaaagct
     1141 ctaaatttga ttctggataa taaaaataca cctattgaag ctgaagtata ttcagtaatt
     1201 tacaacagca aaaaaggatt taacattttt gtaaaaatat cagatagctt atatggcaaa
     1261 gcgctggaag aacatgatgt aaaagtcaaa ataaagcttg ttgctgatga tgaatctaaa
     1321 aaagaagtgt catcaagaac aagccttgtc attggcatta tagctggaat agttgttatt
     1381 gcagtaattg catttttaat attgtcgtga aattatttta taattttcct tcgcgttcca
     1441 atttttgaat atcctgggga gtaattggtt tatctttacc aagaattttt gatgctttgt
     1501 ttaatttttc aatcatgtca tcgtctgtta tgattttgtc gcgcgaagca tcaatatctt
     1561 cttttgattg agggggaatg tttaaataaa tttttgcata tacttcacga ttaccagggt
     1621 attgacctga aagagtaatg gtaagattac atgttgattt tgggagcaaa tatcgtgaaa
     1681 tttttaataa ttgtttaacg gtagatctgg gggcaatata cgttgattct aatagttgat
     1741 caggatttat atttttataa tcaggagaca tctcagtatt gcatggcatg cattccagaa
     1801 ccgcattagt aaatgacact gcttcatcaa aaatattttt catggttact tcaactgtta
     1861 tagtggaact atctatcgaa ggaaatttat tgtagagtac aggtattatt ggattcccca
     1921 tgaggtttga caaatagtgt acattgggga atgaaatact attcctgcct gttgcggtaa
     1981 tattgtttgc atcagcaact gttaccttta ccaaaaaact tgattgtaat cctttttgat
     2041 cacgactggc atagttatgg gtgatatatc cgttttgagt agtagcataa gtaccatcgc
     2101 caaaatccca ttcatacgtg catttccctg atagcccttt ttgcgaggtt acttcaaata
     2161 ttgcagattc tggctctaaa ttgtgtaaac gtgcacttaa tactacaagc ggtttatcag
     2221 ggcattctac aacagtaatc tgaactttgc gcatatctat atgtttacct tcgtcacgca
     2281 ccgttacata aaattctcgc tgtcctgctt tgttaaatcg catgattgca ggattgccca
     2341 atttattgct tatgcgatac accagatgag catctgatcc aaatggattt tttgcaatga
     2401 ccgttacttt aaaatcttcg ccggcacaga cgggattttt ttcaagtatg atatctttta
     2461 taaagtttgc ctgctctatt tcatatgttt caactttttc aggatcaacc ggcggcaatt
     2521 gttccgattg ttcctgctgg gacatgaatt gaaaaatatt taatagcgat tgatttttgg
     2581 aaggatcatc attttttaat ttatcattat tactgtgtat caaaaaaata aatattaaca
     2641 tgactgcggc aataattcca ataatgatat atatacgttt catacgtttt acctgatggt
     2701 ataaatttta aaataagata aggatttaaa taaaaaatat caacaaaaat tttgttcgat
     2761 agttactcat aaaaacttat ctcacatcat tctttcacta taacattgat catatgaaca
     2821 actgccacta actataaatg aatttgttat aataaaaaaa tcccgggcat aaaaaacccg
     2881 ggatgttgta ttatagcaga ttaatccacc cagtaatagt atccggaggt aataaccaga
     2941 ggatcaacct gtccatagta tgaagaggag gtagtttgca cacctgtatt tttccccgat
     3001 ggattggtat aggtataaag caacagatgg tcaggtgcat tggcggttgc ggatatttta
     3061 ttaatatagc ttttccagta atctgaagtg tcagttgatc tatcccagcc aaacgtgttg
     3121 acaatctggt ttgctatctt ttcatcggca ccagttccaa aaagtccctc aatatcaata
     3181 ataaaagatc caaatccgcc ggcttcatca cggaccatat ttttgaccga gttgtataaa
     3241 ctatagccgc attgtgatac caatgatgga ggaatatggg caacattcat tgttttgcct
     3301 gcaaagtaat tagcatacca gcaggtgcct gagcagtgat tgcctcgccc ttttatgtaa
     3361 taatttacat aatctaactg gtacatgtta acatatgcat tcacacggta atatccttta
     3421 acatacaaca ttttgtcagc agcgttaaca agatattgtc ggtatagtgc ttcattagca
     3481 gaagctggct tgagtacagc accaccagca gcagcaaaat tttttgttac attataggtt
     3541 gtgtcaatat cctccgttaa aatgccaggc atgccgttgc tcaatttatc cggattcatc
     3601 cttttgggtg ttgtttgaat aaaccatatt ttgttgtatt caatagggat atcgctaaca
     3661 tacatggtgt tgtggcgaat gttaaatcca ttatcaacag tcatcccgct atggctccag
     3721 tagcaaccca atattgcaag taaatccata ataaatcccg gactggttga aagaataaca
     3781 tctcccttct gggcattaat gctcattggt ttataaaccc attgccctgc aaagattgtt
     3841 gatgttgtaa acacaataag cataataagg catatttttg aaataaactt cattggaacc
     3901 tccttttgat caatatataa ttcaaaatca tagaattgtg ctcatcaaaa gtgagtgaat
     3961 tatcactcac tttttaataa taaacaccct cgaatctaaa atgagttgat tacaaaaaaa
     4021 agtcaattca taaaccgact attaggtata aaataaaaaa tttttttaac tatttgttta
     4081 attgtattac ataaaaaaat gatttatata attcaattaa aaataaagtg aaagctttat
     4141 tgtaataata gtaagtgata tatgatgttg gtaacaagag gatacatacg attatttttg
     4201 atttacaaat tctgatgtgc tacatatatg acataaaaat aatttttgta ataatgtaat
     4261 cagttgaatg gtttttattt aaactttaca ggaggagtcc atggcttata aaattactga
     4321 agattgtaca atgtgtggaa catgtgcttc tgaatgttca tctcaggc
//
LOCUS       DAOOJH010000148         4355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15734_length_4355_cov_10.034419, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000148 DAOOJH010000000
VERSION     DAOOJH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15734_length_4355_cov_10.034419"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1195)
                     /locus_tag="PLJ75_11985"
     CDS             complement(233..1195)
                     /locus_tag="PLJ75_11985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPD05975.1"
                     /translation="MYQKKKANHYRRGNWYGKPYLFFGDYLFARYNTKIFKLSINADL
                     KCPNCDGTINSNGCIFCSEGSASPTANSTQSIHQQMQSAIDGFHRGLYAQKYIAYFQA
                     YTNTHASVAVLKQLYDTALQFDNVIGLMVGTRPDCLPDDVLDLIAQYKKENFELWLEI
                     GLQSVHDKSLQWLQRGHTYSQTVDAIHRAVMRGIDVCVHVILGIHGESWDEMMETANK
                     ISRLPVKGVKIHHLHVIKGTPLAMIYNTGALSLLSCKKYVSYVCDFIERLRDDIIIHR
                     LSGDQLKDMLVAPSWGLHKGTVIKAIEDELKRRGTYQGFLCDNY"
     gene            complement(1164..2822)
                     /locus_tag="PLJ75_11990"
     CDS             complement(1164..2822)
                     /locus_tag="PLJ75_11990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPD05976.1"
                     /translation="MKKSVLLCILLICLMIPCAVFAEQDIIATILNGEQSKERADEII
                     QIAGFIDELYSNLPQRIKNGQKLVLFFDPAHGIDDDGKWEGEKTGRLSCTGLPEEFYS
                     IAISRKLYTLLKKNPYIQVASTKDYIDALEGNSDSYNNITFKETVELARNARAALVIS
                     EHLNNTASLFKAYGLVNVKGIHVTYSNNTAYLTHIKEEHKGFLTLYNKFDTSGFSYKL
                     ASAVKGKLVANGLRVNSWNFGTVADDRFSYFVNYPISVIFESGFISNPDDETMLRNPD
                     YQQLIAKSHYESILETIATTFGVDLSGFWGPRIIDTDFQKNLTLLKLSRIACNYISKN
                     DTSKGLYVLQILLNQCDPEKDTAVIENITDMKYRIERSQTLYTKALQLKKKGQIRKAK
                     ISMQKAISLISRSPVFYEMRKAYRTEYGVLKETSPQKDYALQHPIPAKGVNPSPSSIT
                     TPVLIVLKNDITVSEAIYNALRCDDAMLKKLTQQFASSKIKAGKKRFENPTKEGIYIV
                     QLTPKGNIKSVKRVTIVKLNPYKYQNQMYLKNSYCVSKEKSKSL"
     gene            3268..3846
                     /locus_tag="PLJ75_11995"
     CDS             3268..3846
                     /locus_tag="PLJ75_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05977.1"
                     /translation="MKKFFATIVIVFITSSFALANYYVDGMFMINDTGDAKSQTGVAL
                     KLATSLRNDINLFFKPLFSQTTEHSNSINETDYRQIQGLFGAEYIYSLPQYPIAISFS
                     AGVGISRTEIEPKDDTSGLEELKETGMAYGLWLGGQWLLTQWVAPFIELGYSKSLYNN
                     DLDGASIGGMQIAIGVRFTIWGKNKTFTSDYE"
     gene            complement(3852..>4355)
                     /locus_tag="PLJ75_12000"
     CDS             complement(3852..>4355)
                     /locus_tag="PLJ75_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05978.1"
                     /translation="VYSVFMDSGYTVIALQGNELDSYYAHDETIMNYFQYISTNDNSY
                     DFITDWTMFKIATGAWQGKKNSQWYEDMRKFYEIYDIHYKIFKNTILEKYKTIYRGCD
                     YVLVVVFNDARSSCWVRAIDINAKQLVFMHPNISAAGMGKDIQKTTRELIQVMQTPTS
                     LPQKAKK"
BASE COUNT         1301 a          838 c          743 g         1473 t
ORIGIN      
        1 aacttgcact ttttttgctt ttaatacctc ccttttttaa gggcaaattt aatccagcgc
       61 cctgcattaa cttttgcgcc tgtcatggat gcaaagccgt catcatagcg ttgaaaataa
      121 caccgtaaaa tgaatgatac aaaatcgttg tttttgtatc aatacgtttg ttaatgctct
      181 ggctttaata ctactatagt aaaattctgt atacaatgca atcacaaaat tttcaataat
      241 tatcgcacaa aaaaccctga taagtgccgc gtctttttaa ttcatcctca attgctttaa
      301 tgactgttcc tttatgcaaa ccccatgaag gtgccaccag catgtctttc aactgatctc
      361 ccgataacct gtgaatgata atatcatcac ggagccgctc aataaaatcg catacgtatg
      421 aaacatactt tttgcatgac aaaagggaaa gtgcacctgt gttatatatc attgccaatg
      481 gcgtgccttt gataacatgg agatgatgaa tctttacccc ttttaccgga agcctgctta
      541 ttttattggc ggtttccatc atttcatccc agctttcgcc gtgtataccc agaataacat
      601 gtacgcatac atcaatacca cgcataacag cacggtgtat ggcatcaacc gtctggctat
      661 acgtatgccc gcgttgcagc cactgcaggc ttttatcatg tacactttgc aaacctatct
      721 caagccacag ctcaaaattt tcttttttgt actgtgcaat gagatcaagc acatcatcag
      781 gaaggcaatc cgggcgtgtc cctaccataa gcccaatgac attgtcaaac tgcaatgctg
      841 tatcataaag ctgttttaac actgccaccg atgcatgggt attggtatat gcctgaaaat
      901 aagcaatata cttttgagca tacaagccac gatgaaagcc atcaatggca gattgcattt
      961 gctgatgtat tgattgtgtg ctatttgctg taggtgatgc tgaaccttcc gaacaaaaaa
     1021 tacacccatt actgttgata gtaccatcac agttggggca ttttaaatca gcatttatgg
     1081 aaagtttaaa aatttttgta ttatacctgg caaataaata atccccaaaa aaaaggtacg
     1141 gcttgccata ccagttccca cgcctataat gatttgcttt tttcttttga tacacaatat
     1201 gaattcttta aatacatctg attttgatat ttatacggat taagtttaac aattgttaca
     1261 cgctttacac ttttaatgtt ccctttaggg gttaactgta ctatgtatat accctctttt
     1321 gtgggatttt caaatctttt tttgcctgct tttatttttg aagaagcaaa ctgctgcgtt
     1381 agctttttta gcatagcatc atcgcatctc agggcattgt agattgcttc gctaactgtt
     1441 atatcgtttt ttagtacaat gagtactggt gtcgtaatgg atgatggtga cggattaacc
     1501 cccttagcag gtataggatg ttgcaaagca taatctttct ggggagatgt ttcttttaaa
     1561 acaccatact ctgttctgta cgctttcctc atttcataaa aaaccggtga gcggcttatt
     1621 aaacttatag ctttttgcat gctaattttt gccttcctga tctggccttt ctttttcaat
     1681 tgcaaagctt tagtatataa tgtttgtgaa cgttcaatac gatacttcat atcggtaatg
     1741 ttttcaatca cagccgtatc tttttcagga tcgcactgat ttaataaaat ctgcaaaaca
     1801 tataatcctt ttgaagtatc attttttgat atgtaattac aggcaatccg tgacagttta
     1861 agcaacgtaa ggtttttttg aaaatcagtg tcaataattc tgggacccca gaatccagat
     1921 aaatcaacac caaaggttgt ggcgatagtt tccagtatcg attcatagtg ggattttgca
     1981 atcagttgct gatagtcagg attgcgcagc attgtttcat cgtcaggatt tgatataaat
     2041 ccggactcaa agattactga tataggatag ttgacaaaat acgaaaatcg gtcatcggct
     2101 actgtaccaa aattccagct attgacacgc aaaccatttg ctacgagctt acccttaacg
     2161 gcgcttgcca gcttatatga aaatccgctt gtatcaaatt tattatataa cgttaaaaat
     2221 cctttatgtt cctctttaat atgggtaaga tacgctgtgt tattactata ggttacatgt
     2281 attcctttca cattgacaag cccatacgct ttaaacaatg atgctgtatt attaagatgt
     2341 tctgaaatta ccagtgctgc tctggcattt cgggctaatt caactgtctc cttgaaagtg
     2401 atattattat aactatcaga gttaccctct aatgcatcga tgtaatcctt tgtacttgct
     2461 acctgtatat atggattttt ttttaaaagt gtataaagct tccgtgatat agctattgaa
     2521 tagaactctt cgggcaatcc tgtacagctt aaacgtcctg ttttctcacc ttcccattta
     2581 ccatcatcgt caatcccatg tgctggatca aaaaatagta ccagcttctg gccattttta
     2641 atcctttgtg gaagattgct gtataattca tcaataaatc ctgctatttg aattatttca
     2701 tcagcacgtt cctttgattg ctctccattt aaaatggtag cgataatgtc ttgctcagca
     2761 aatactgcgc agggaatcat caagcaaatc aataatatac atagcaacac actttttttc
     2821 ataatcaata actatcgctt tatatattga tacattatac agttataatg tcgaataaat
     2881 ttccattcag gttaaatgag ttatacaata ttttcgtcat gacacaactt tcatggctat
     2941 aatggaatct gcgccatggt agtaagttaa atatggataa tgtaaagtgc tataaatttt
     3001 ttcatcaact tttttaaaaa ttttacattt tttatacatg ggaagataaa aaaaatttga
     3061 taatgtttta ttactcctgc ttttgctcta cagctaattt tgccgataat ttttgtattg
     3121 acattgtttc aattatagta aacaatgtta ctgttttatt agcaccacac catcattagt
     3181 tttccattgc ggtgcgatag ttagtgtata attatttttc acgttgatat cattgacagc
     3241 gttgattatt tccatacgga ggatcatatg aaaaaatttt ttgcgacaat agtcattgta
     3301 tttataacct caagctttgc attagccaac tactatgttg acggcatgtt catgattaat
     3361 gatacaggcg atgcaaagag tcaaacaggg gtggctttaa aattagcgac cagcttaagg
     3421 aatgatatta atctattctt taaaccattg tttagccaga caactgaaca ttccaatagt
     3481 atcaatgaaa ctgattaccg gcagattcaa gggctttttg gagctgaata tatatactcg
     3541 ttacctcaat atccaattgc aataagtttt agcgccgggg taggcatttc aagaacagag
     3601 atagaaccta aagacgatac ttcaggatta gaagaattaa aagaaactgg tatggcgtat
     3661 ggattatggt taggcgggca atggttatta acacagtggg tagctccttt tattgagctt
     3721 ggttactcaa aatcactcta taataacgat cttgatggtg ccagcatagg cgggatgcag
     3781 atagcgatag gtgttcgttt tactatatgg ggaaaaaata aaacgtttac cagcgattat
     3841 gagtaatctg attatttctt tgctttctgc ggtaatgacg ttggtgtttg cattacctga
     3901 ataagctccc tggttgtttt ttgaatatcc ttgcccatac cggcagcact gatattggga
     3961 tgcataaaca ccagctgctt tgcattaata tcaatagccc gcacccagca acttgaacgt
     4021 gcatcattaa ataccactac caatacatag tcacagccac gatagatggt tttatatttt
     4081 tccagtatgg tattcttaaa tattttataa tgaatatcat atatttcata gaacttccgc
     4141 atgtcctcat accactggct attctttttc ccctgccatg caccggtagc aatcttaaac
     4201 atggtccaat cagtaataaa atcatacgag ttatcattag tacttatata ttgaaaatag
     4261 ttcataatcg tttcatcatg ggcatagtag ctatcaagct cattgccctg gagagcaata
     4321 accgtatagc ctgaatccat aaatactgaa taaac
//
LOCUS       DAOOJH010000149         4340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15813_length_4340_cov_9.653442, whole genome shotgun sequence.
ACCESSION   DAOOJH010000149 DAOOJH010000000
VERSION     DAOOJH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15813_length_4340_cov_9.653442"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1289)
                     /locus_tag="PLJ75_12005"
     CDS             complement(99..1289)
                     /locus_tag="PLJ75_12005"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD05979.1"
                     /translation="MFINFFFNLKAQGVPVSIHEWMALHEALVLNLNDVSLTKFYYMA
                     RAILVKNEVLYDRYDIAFLDTFKDVETTDELLEQILEGLKKVKELHLTEEEKRQVEKL
                     NLDEVLRNFEEQLRQGHYKNHVGGNKAIGTGGRSTQGAWGYNPAGIRIGQGVSRHKSA
                     VQIAEKREFKNYSNQIILDTRQMRVALSHLRSLLPIGPEEHLDVEQTVDATSKNAGEL
                     ELIWKKKEKKAAKLILLMDAGGSMTPYARLVERLFSAASSQISKLKYFYFHNCIYQDL
                     WKDIERSESISTVDFLKNTDKDYKLIIVGDAEMAPSELTWANGAIDYWYHNDIPGIVW
                     LQRLRETFKYSLWLNPIPQRSWDYIQTTRMIKNIFPMFELTLEGLDEAIKHLISGKVL
                     QKAV"
     gene            complement(1279..2133)
                     /locus_tag="PLJ75_12010"
     CDS             complement(1279..2133)
                     /locus_tag="PLJ75_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPD05980.1"
                     /translation="MNYFTGTDEYVLSQELKDNVNVAIALGRPLLVKGEPGTGKTLLA
                     HSIARGLGKRLLVWNIKSTTKAQEGLYVYDTVQRLNDARFGGKDVSNIKQYIKLGKLG
                     ESFASEEQVVLLIDEIDKADIEFPNDLLHELDIMNFYIPETGETIAAQHRPIVIITSN
                     SEKELPDPFLRRCVFHYIAFPDEELMERIVRVHFPDIEKKLLRECLKKFYWIRQFDML
                     RKKPSTSELVDWIQALKIGGVSIKKIEDEIPFIGTLLKKKEDIDVVANSGKTHYAYNS
                     SIRTRDVY"
     gene            complement(2246..2641)
                     /locus_tag="PLJ75_12015"
     CDS             complement(2246..2641)
                     /locus_tag="PLJ75_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPD05981.1"
                     /translation="MVSFQVSTGRKEELIDITAKVAEAVSKSSIDSGLCVVYVPHTTA
                     AVTINENADPAVSKDILLGLSHMKLESLHFAHLEGNSPAHIKSSLIGCSVSVIVEKGR
                     LQLGTWQGIFFCEFDGPRTRKVMVQCIGT"
     gene            2851..>4340
                     /locus_tag="PLJ75_12020"
     CDS             2851..>4340
                     /locus_tag="PLJ75_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05982.1"
                     /translation="MKKVDRTKENIKVDELDDKTRKQMFDKFVQSGGKIVNEHDSQQI
                     KLKKRVPTVTTSTKRTYPSKNTVKESKQPQIKHEVVFETWLQKKLSHLFIRLRLLFLG
                     VTGFSADTFKHRFCYNVIHDYKTALLQFQVQYFYLFNPQYSKQIIAKLDAVNPLYYEL
                     IVMISNIFDRTRFTEIEEIIQQNKPVSTAKLKNHIIPIYRSLYILYPYIELIELAYRT
                     AYDTLGTIEKQKSSVYYERKKFIRNSLYTTFYKLLPRLHIIICYYYNVYIDITNPYVQ
                     TIMQITQDVLPGKRKAIQSNQEASQQQEAETQEQTTQTPPHIIKGISLMNISTNDIMT
                     LFKNNSSVQPSPADKAAITYVLFKKFDDEFSIVLTTNKIKYTIQFTESGKIDYKNRLA
                     DLYNELRKITESFQEYMHILETYERIRSEKPMSNAQYIEYTKILTQLEKKKKVQSKNM
                     RMVTKSIMDAITAELDILIKDMDEEHKIVDNPQDFLEFDINVESAHL"
BASE COUNT         1425 a          927 c          701 g         1287 t
ORIGIN      
        1 cttattaacc ctgatcacca aaaagcacgt gaatatcgtg aacgggcagt aacaaaattg
       61 gaattgcaac aatcacttca gtaaaaaggc ataacctttt acacagcttt ttgcaatacc
      121 ttgcctgata ttaaatgctt gattgcttca tcaagccctt ccagggtaag ttcaaacatg
      181 gggaatatat tttttatcat ccgcgtagtc tggatataat cccatgaacg ctgcgggata
      241 gggttaagcc atagtgaata cttaaatgtt tcacgcaacc gctgcagcca tacaatgcca
      301 ggaatatcat tatggtacca ataatcaata gcaccgtttg cccatgtcag ttctgatggc
      361 gccatttcag catcgcctac aatgataagc ttatagtctt tatcggtatt ctttaaaaag
      421 tcaacagtgc ttatgctctc tgagcgttca atatccttcc ataaatcctg ataaatacaa
      481 ttgtgaaaat aaaagtattt taatttactg atttgcgaag atgctgctga aaaaagcctt
      541 tcaaccagcc tggcataagg agtcattgaa ccgcccgcat ccatgagtaa aataagcttt
      601 gctgcttttt tttctttctt tttccatata agctccagct cacctgcatt tttactggta
      661 gcatcaacgg tttgctcaac atccagatgt tcttcggggc caataggcaa taatgagcgc
      721 agatgcgaca gcgccaccct catctgccgt gtatcaagga tgatctgatt ggaatagttt
      781 ttaaattcac gcttttcagc aatctgcact gcgcttttat gccgtgatac accctgccca
      841 attctgatac ctgcaggatt atatccccat gccccctggg tggaacgccc gccagtacca
      901 atggctttat tgccaccaac atgattttta taatgtccct gccgcagttg ttcctcaaaa
      961 ttgcgcagaa cttcatctaa attgagcttt tcaacctgac gcttttcctc ctcggttaga
     1021 tgaagttcct ttaccttttt taagccttct aaaatttgtt caagcaactc atcagtggtt
     1081 tcaacatcct taaatgtatc aagaaatgca atatcgtatc ggtcatacaa tacttcattc
     1141 ttcacaagaa tagcccgtgc catataataa aatttagtca ggcttacatc gtttagatta
     1201 agcaccagcg cctcatggag agccatccac tcatggatgg aaacaggcac gccctgcgct
     1261 ttaaggttaa agaaaaaatt aataaacatc gcgcgtcctt atgctgctgt tgtaggcata
     1321 atgtgttttg cccgagtttg ctacaacatc aatatcttct tttttcttta agagtgttcc
     1381 aataaatggt atctcatcct ctattttttt tattgataca ccgcctattt taagagcctg
     1441 tatccagtca acaagttcgc ttgttgatgg ttttttccgc aacatgtcaa actgccgaat
     1501 ccagtaaaat ttcttcaaac attcacggag cagcttcttc tctatatccg gaaaatgtac
     1561 ccgtacaatc ctctccataa gctcttcatc aggaaatgca atatagtgaa atacacagcg
     1621 ccgtaaaaat ggatcgggca gttccttttc gctattggag gttataatga caattggccg
     1681 gtgctgtgct gctatcgtct cgcctgtttc aggaatataa aaattcatga tatcaagctc
     1741 gtgcagaagg tcattgggga attcaatatc cgccttgtct atctcatcta ttaaaagaac
     1801 cacctgttct tctgatgcaa atgattcgcc caacttcccc agcttaatat attgtttaat
     1861 gttggaaaca tccttaccac caaaccgcgc atcgtttaaa cgctgcaccg tatcatatac
     1921 atataaacct tcctgagcct ttgttgttga ttttatattc caaaccaata aacgttttcc
     1981 caatcctctg gcaatggaat gcgccaggag tgttttcccc gtccctggct ccccttttac
     2041 caataatggc ctgcccaatg ctatagcaac atttacatta tccttcaatt cctgcgaaag
     2101 aacatactca tcagtaccgg taaaataatt catcgtgcac ctcatcatat tttatacgat
     2161 agttctttat actatttata ctatttatac tatcctattg tactattgta gtacaggaaa
     2221 gcataaaaaa caaaaaatta cactgttatg ttccaataca ttgcaccata accttacggg
     2281 tgcgcggacc atcaaattca caaaaaaata tcccctgcca tgttccaagc tgtaagcgcc
     2341 ctttctcgac aatgacactt actgagcatc caatcagtga cgatttaata tgcgcagggg
     2401 aatttccttc taaatgcgca aaatgtaacg actctaattt catgtgcgat agtcccagta
     2461 aaatgtcctt ggacactgca gggtccgcat tttcgttaat agtgactgcc gctgttgtat
     2521 gaggaacata cacaacacat aagccgctat caatagatga tttactaaca gcctccgcca
     2581 ctttagcagt gatatcaatg agttcttctt ttctgcctgt tgaaacctga aatgaaacca
     2641 ttaatgtgcc tcaattatta tagtatagta tataatagta attctttaat acaatgtaac
     2701 aattaataca gttatagcgt tttttttaca agattttttc agattgttat ccatagcagt
     2761 gcataatgca accacatata aaataaaacc tgtaatgttg ttgacataca cattacatgg
     2821 tatgtatatt tattaataga gcaaagcatc atgaaaaaag tagatagaac aaaagaaaac
     2881 atcaaggttg acgaattaga cgataaaacc cgtaaacaga tgtttgataa gtttgtacag
     2941 tcgggcggta agatcgttaa tgaacatgac agccaacaga ttaaattaaa aaaaagagta
     3001 ccaacagtaa ccaccagtac aaaaagaaca tatccctcta aaaatactgt taaggaatca
     3061 aaacaacctc aaataaaaca tgaagtagtg tttgaaacgt ggcttcagaa aaagctttcg
     3121 cacctgttta tacgtttacg gttactattt cttggtgtta ccggattttc tgctgacaca
     3181 tttaagcatc gattttgtta caacgtaata catgattata aaacagcact gctgcaattt
     3241 caggtgcaat atttttatct atttaatcca caatacagca agcagattat agcaaaatta
     3301 gatgctgtca acccccttta ttatgaactt atagtaatga tatccaatat atttgacaga
     3361 acgcgattca ccgagatcga agaaataatc cagcaaaaca agccggtaag tacagcaaaa
     3421 cttaaaaatc acatcatccc aatatacaga agtttgtata ttctttatcc atatattgaa
     3481 ttaattgaac tggcataccg tactgcatac gatacgctgg gaactatcga aaaacaaaaa
     3541 tcttcagtat actatgaaag gaaaaaattt atccgcaaca gcctgtacac aacattctat
     3601 aaattacttc cgcgtcttca cataataatc tgttattact acaatgtcta cattgacata
     3661 acaaatccct atgtacaaac tatcatgcaa ataactcagg atgttcttcc cggaaaaaga
     3721 aaagcaatac agtcaaacca agaggcatct cagcaacaag aagctgaaac acaggagcaa
     3781 accacccaaa cacctccgca tatcattaaa gggatttccc ttatgaatat atcaaccaat
     3841 gatattatga cattatttaa aaataattcc agtgttcaac ccagcccggc tgataaggct
     3901 gctattacgt atgtgctttt taaaaaattt gatgatgaat tttctattgt gctcactacc
     3961 aataaaatta aatatactat ccaatttacc gaatcaggca agattgatta taaaaacagg
     4021 ctagctgacc tttacaatga actgcgaaaa attactgaga gttttcagga atatatgcac
     4081 atactggaaa catacgaacg catacgttca gaaaaaccca tgagcaatgc acaatatatt
     4141 gaatatacca agatactgac acaacttgaa aaaaagaaaa aagtgcagtc aaaaaacatg
     4201 cgcatggtta ccaaatcaat catggatgca attacagcag aattggatat cttaataaaa
     4261 gatatggatg aggaacataa aattgttgat aatccacagg attttcttga gtttgatatc
     4321 aatgtggaat cagcacatct
//
LOCUS       DAOOJH010000150         4329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_15869_length_4329_cov_10.062237, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000150 DAOOJH010000000
VERSION     DAOOJH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15869_length_4329_cov_10.062237"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLJ75_12025"
     CDS             complement(<1..401)
                     /locus_tag="PLJ75_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPD05983.1"
                     /translation="MDISVHLMKLTGLAIDMLSKLSNATIRVHDAERIPKAPVVFVIN
                     HFTRMETFFLPNVIYKITGKYLYSLAHHSFFTGSFGKFMEKVGAVSTKDPLRDAKFIG
                     ALLRGDMDCIIFPEGQMIKDKRFIERGKYMV"
     gene            complement(412..834)
                     /locus_tag="PLJ75_12030"
     CDS             complement(412..834)
                     /locus_tag="PLJ75_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPD05984.1"
                     /translation="MTAYRNPVPTVDIIILLEDEHGTVKHDEIVLIKRKNPPYGWALP
                     GGFVNYGETLEQAAIREAKEETSLDVILLRQFHTYSDPARDPRQHTISTVYVARAKGK
                     PEANDDAGEAEIFTIHNVPVDMAFDHAQIIDDYKNNRY"
     gene            complement(838..1971)
                     /locus_tag="PLJ75_12035"
     CDS             complement(838..1971)
                     /locus_tag="PLJ75_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD05985.1"
                     /translation="MKQSITYNAKQKRWGLLLYGVLALLIITLFVVMYVFIYYAINHS
                     LLGHSYLFLFVLFVLMLVAGIYSTIKFLVITTNEIVSSKHELALKDVIIEHSNEIIIL
                     LDNIGKILSINPALTTILNFDKEELIQQPFRAVLYEKSFEDSVRLRDLLLSRFKDVFK
                     GYEAEIVLPCKVQHYAEIKSIAFKLVPIFKDEQLQYILAIGRIIQSDYLTSNYLKSEF
                     AEFILDNNIRMVNMLSYRLTRNLETYMEKKEIIRIQLGLQEAIINAIEHGNLEIDFKT
                     KSILKKKNGNYWDHVIDRCNKEYLEKRKIKVQYYLSPDHVKYIITDEGKGFEWEHYIT
                     TLPDGNLINNYHGVGLTMLQDIFNVSFNEKGNEITMIKYFNKR"
     gene            2251..>2724
                     /locus_tag="PLJ75_12040"
     CDS             2251..>2724
                     /locus_tag="PLJ75_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPD05986.1"
                     /translation="MEHNTGNIIVYDTTLRDGAQTFGISFSLQDKIRIALELDALKID
                     YIEGGWPGSNPKDNLFFSEIRNASINHSKIAAFGSTRRPKMKLAEDELLHNLIQSNAH
                     TLTIVAKSWDFHVTDALNITLEENLNLVYDTIAYCKDKGFEVFLDAEHFYDGFKAN"
     assembly_gap    2725..2824
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2825..3553
                     /locus_tag="PLJ75_12045"
     CDS             <2825..3553
                     /locus_tag="PLJ75_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPD05987.1"
                     /translation="HNEHAPYVGDNAFAHKGGIHVSALKHNTATYEHINPELVGNRRK
                     VIISELAGKSNIQVKAEELGISLNKIKLSEAILHRIKVLEDQGFQFEAAEGSFELLIR
                     KSNGEYTPFFTLKGFRVITEKNESGKVSCEATIKVDVDGQIEHTAANGNGPVQALDNA
                     LRKALESFYPEINELQLSDYKVRVLDEKEGTGSPVRVLIDHKRHVDHWGTVGVSTNII
                     EASWQALVDGIEYMLLKTRNNTAH"
     gene            3504..>4329
                     /gene="murI"
                     /locus_tag="PLJ75_12050"
     CDS             3504..>4329
                     /gene="murI"
                     /locus_tag="PLJ75_12050"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPD05988.1"
                     /translation="MVLNICSLKQETIQHINIQDITIIMNSQPIGIFDSGIGGLTVCK
                     AIHEMLPGENLLYFGDTARFPYGTRSPETLIRYARQITAYLLSRNVKMIVIACNTSSA
                     AALPILEQENTIPIIGVINAGARAACRRVYNNIIGVIGTRATVKSGSYQKAIHAINPE
                     VKVIQQQATLLVSLIEEGWIDEEVTKLTITKYLQHMIDEKTKTVILGCTHFPILKATI
                     RKVYPQLDLVDTSEEIAREVKEILVKKNLANNSSSGKIELYASDITDTMHYLKSLFF"
BASE COUNT         1380 a          832 c          726 g         1291 t
ORIGIN      
        1 taaaccatat atttgccacg ttctataaac cttttatcct ttatcatctg cccttcaggg
       61 aatattatac aatccatatc ccctctgagc aaagcaccaa tgaacttagc atccctcagg
      121 ggatcttttg ttgaaacagc accaaccttc tccatgaatt ttccaaagct gcctgtaaaa
      181 aagctgtgat gagctaatga atagagatac tttcccgtta ttttataaat aacattggga
      241 agaaaaaatg tttccattcg ggtaaaatgg tttatcacaa atacaacagg cgctttagga
      301 atcctttctg catcatgtac ccgtatagta gcattgctta atttagaaag catatcaatt
      361 gccaatccgg ttaatttcat aagatgaaca gaaatatcca ttgatactcc ctcaatatcg
      421 gttattttta tagtcatcaa ttatctgtgc atgatcaaaa gccatgtcga caggcacatt
      481 gtgtatagta aaaatttctg cttctccggc atcatcattg gcttctggtt ttcctttagc
      541 cctggcaaca taaacagtgg aaatagtatg ctgccgcgga tcccgtgcag gatcagaata
      601 ggtatgaaat tgcctcagca atataacatc cagcgatgtt tcttcctttg cctcccggat
      661 tgctgcctgt tccagcgttt caccataatt cacaaagcca ccgggtaaag cccacccata
      721 cggtggattt tttcgtttta ttaagacaat ctcatcatgc tttacagtac catgttcatc
      781 ttcaagcagt ataataatgt caacggtagg tacaggattg cggtaagcag tcatacatca
      841 ccttttgtta aaatacttta tcatggttat ctcattacct ttctcattaa acgacacatt
      901 gaagatatcc tgtaacattg ttaaccccac gccatggtaa ttgttgatta aattaccatc
      961 aggcagcgtt gtaatatagt gctcccattc aaaaccttta ccctcatcag ttatgatgta
     1021 tttcacatga tcaggggata aataatactg taccttaatc ttccgcttct ctaaatactc
     1081 tttattgcat ctatcaataa catgatccca ataattgcca ttctttttct ttaaaatact
     1141 tttggtctta aaatctatct ctagattacc atgttctatc gcattgatga tggcttcctg
     1201 cagccctaac tgtatacgta tgatctcttt tttttccata taggtttcta aatttcttgt
     1261 taatctatat gacagcatgt ttaccatgcg tatgttgttg tccagaataa attctgcaaa
     1321 ttcgcttttg agatagttac tggtaaggta gtcagattgt ataatccttc ctatagcaag
     1381 gatatattgc aactgttcat ctttaaaaat gggaactaac ttaaatgcaa tagattttat
     1441 ttcggcatag tgttgcacct tacacggcag aacaatttca gcctcatatc ctttaaatac
     1501 atctttaaat ctgctgagca aaagatcccg caatctcact gaatcttcaa atgacttctc
     1561 atataaaact gccctgaatg gttgctgtat caattcttct ttgtcaaaat taagtatggt
     1621 tgttaatgca ggattaatgc ttaaaatttt tccaatatta tcaaggagta tgataatttc
     1681 attggaatgc tctataatga cgtcctttaa tgccagttca tgtttactgc tgacaatctc
     1741 atttgttgtt ataaccaaaa attttatagt agaataaatc cctgcaacta acatcagaac
     1801 aaataaaaca aaaagaaata aataagaatg tcctaacaat gaatgattga tggcgtaata
     1861 tataaataca tacattacta caaataaggt aataattaat agagccagaa ctccataaag
     1921 aagcaaaccc catcgctttt gtttagcatt atatgttata ctctgtttca tacttcatta
     1981 tggcaaacga tattaattat ttactatagt aaccactata atcaatatta attataaatt
     2041 tcaagcaatt tgcccatttt gtcaaaaaaa tagcttgaat attttacaca gttcccatta
     2101 tatgcattat cgtttacgtt gtcattcaat gcaaaaaaaa tataacactc cattaaaatt
     2161 atttaataat atttgtactg ccagaaaaag tgttatatat taattgtatg ctaataccat
     2221 ggtcaacagt taaagtaccc gtgagctatt atggaacata acacaggaaa catcattgta
     2281 tatgatacta cattgcgcga tggagcccaa acgtttggta tttcattttc ccttcaggat
     2341 aaaatccgta ttgcacttga attagatgca ttgaagattg actatatcga aggcggctgg
     2401 ccaggatcca atccaaagga taatcttttc ttcagtgaaa tccgtaatgc ttctataaat
     2461 cattctaaaa tcgcagcctt cggttcaacc cgacgtccta aaatgaaatt agcggaagat
     2521 gaacttttgc acaatctcat ccaatccaat gctcataccc tgaccattgt agcaaaatca
     2581 tgggatttcc atgttaccga cgcactcaac ataacgctgg aggaaaattt aaatctggtg
     2641 tatgatacca ttgcctattg caaggataag ggatttgagg tctttttaga tgccgagcat
     2701 ttttatgatg gttttaaagc caacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnncataat gagcatgctc cgtatgttgg cgacaatgct tttgcccata aaggcggcat
     2881 tcatgtatca gcattaaaac ataacaccgc aacctatgag catatcaatc cggaattagt
     2941 gggaaacaga cgaaaggtta tcatctctga gcttgcaggc aaaagcaata ttcaggtaaa
     3001 ggctgaagaa ctggggattt ctttaaataa aattaaatta tcagaagcaa tacttcaccg
     3061 cataaaagtt ttagaagatc agggatttca gtttgaagct gctgagggat cgtttgaatt
     3121 attaatacga aaaagcaatg gcgaatacac ccctttcttt acactgaagg gcttcagagt
     3181 aataaccgag aagaatgaat caggaaaagt atcctgtgaa gcaacaatca aggttgatgt
     3241 tgatgggcag attgagcata cggcggctaa cggcaatgga cccgttcagg cgcttgataa
     3301 tgcactgcga aaagcactgg aaagctttta tcctgaaata aacgaattac aactttccga
     3361 ttataaagtc agggttcttg atgaaaaaga aggcactggt tcgcctgtac gtgtactcat
     3421 tgatcataaa cggcatgtgg atcactgggg tacggtaggt gtatcaacca atattatcga
     3481 agcctcatgg caggcattgg ttgatggtat tgaatatatg ctccttaaaa caagaaacaa
     3541 tacagcacat taatatacag gatattacca ttataatgaa ctctcagcct ataggtatct
     3601 ttgattccgg gatagggggg cttactgttt gtaaagccat ccatgaaatg ctgccaggtg
     3661 aaaacttact atactttggt gatactgccc gcttccctta tggtacacgt tcccctgaaa
     3721 cgctgatacg ctatgcacgt cagataactg catatctttt atcgcgcaat gttaaaatga
     3781 ttgtcattgc atgcaataca tcttccgcag cagcattgcc catactggaa caggaaaaca
     3841 ccatccctat cattggagtt atcaatgccg gtgcacgagc agcctgcaga agagtgtaca
     3901 ataacataat aggtgttatt ggcacacgcg caacggtaaa aagtggatca taccagaagg
     3961 caattcatgc aataaatccg gaagtgaagg taatccagca acaggcaact ctgctggtat
     4021 ctcttattga agagggctgg atagacgaag aggtaacaaa attaaccatc acaaaatatc
     4081 ttcaacatat gatcgatgaa aaaacaaaaa ccgtaatttt aggctgtact cacttcccca
     4141 ttttgaaagc aacaatccgt aaggtgtatc cgcaattaga tttagttgat accagcgaag
     4201 aaatagcacg tgaagtaaaa gaaattttag taaaaaaaaa tcttgcaaat aattcatcat
     4261 caggcaaaat agagctctat gcatcggata tcaccgatac catgcactat ttaaaatcat
     4321 tattcttca
//
LOCUS       DAOOJH010000151         4299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16025_length_4299_cov_7.569746, whole genome shotgun sequence.
ACCESSION   DAOOJH010000151 DAOOJH010000000
VERSION     DAOOJH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16025_length_4299_cov_7.569746"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1213
                     /locus_tag="PLJ75_12055"
     CDS             137..1213
                     /locus_tag="PLJ75_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPD05989.1"
                     /translation="MYANYEQKGSVGGEPLQDVFVISRKDWLIVPYVNYKPETQLAGG
                     AIFLTYFQDIVMNIPPIKLRPTTLQVSVTQTQNGQFMFQVLPEFYFYRDRFHIKNDFQ
                     YMDYYDKFYGIGQDTSDKHNEYYRSRVFAFKNVAEYALVPKLYMGLIYHIDVRETTKA
                     ESGGLIDANLLPGAKEGLVSGLGFTVNYDNRDDIIYTRSGSYANLSVIQYSEYIKSDF
                     NYTNITFDFRYFEPFLFSHILAFQFYTGYIDGEAPFYLLSMLGGDKLMRGLYQGRYRD
                     NDVMVAQVEYRVPLFWRFIACGFAGAGEVAPGMDDFKVDEIVYAWGGGLRFKLRANQD
                     LNVRMDVGFSKNFIGIYATIGEAI"
     gene            1597..2754
                     /locus_tag="PLJ75_12060"
     CDS             1597..2754
                     /locus_tag="PLJ75_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05990.1"
                     /translation="MKRPVEVSTGELIEILDRYLQTQGKVHYAIKIVGYPGVGKSAIV
                     QQIAHKHNYLFIDTRLAFKENVDLGGYPVPDNNQQRMVYFRPRFIPPEKVPEGYNGIL
                     WFLDEANRAHPTVIQTLFQIITERMCGEHALPDKTVIVLAGNLGDLDNTVITDFDDSA
                     LDGRLAVFNLIPDAQNWLHWALEEGIHPAVVRYISTYPERLWDERGVNPNPRGWHQIS
                     DTLYKAYNFRTEEQLVYYLQHTDYKVLQKIIISLIGEVAGTDFILQYVAPRVISSQEI
                     LAGDEQKLQKLIAGDIPAEDMMWALNGALNKLYNDNLHSNGKLGEDIQKELFHVLQFI
                     AYTRADIRVSFMYALVRDCGIFTQIHDALNFEDDPVKKKDLLEKIQVFLDV"
     assembly_gap    2848..2947
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2948..3634
                     /locus_tag="PLJ75_12065"
     CDS             <2948..3634
                     /locus_tag="PLJ75_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05991.1"
                     /translation="KRTIKNKVIAHAKKDEWCTSERSYMDIISCIEKINLSDSSHYSQ
                     HIKSIIDYMSHSNEWYYTHSRFNRRYVAKGIYNAGRAYKQNEIITVAIDISASMMSQK
                     DDIETAFGIVDDLTGKYKVYLVCVDEEYFIPVKDGDALIRANRLTKPYEYKKGDWKLI
                     KSGYGGATFFSSFFNEYLRHHNEMVLVITDGYIYDIDKLKPYPATLWLVAKSRNEAFV
                     PPFGKAIPMV"
     gene            3644..>4299
                     /locus_tag="PLJ75_12070"
     CDS             3644..>4299
                     /locus_tag="PLJ75_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05992.1"
                     /translation="MDEHINTNYNRAQSLYREGIENTAHSLQYFDDLLNCNISEFIIK
                     AGRDWKNFDVDAAMDFFISNNDIEAFYHAGIYWKNFNYERGITVIVEWGNDEYIFRAG
                     RFWKQFDYNRGLSKLIELQSAKYIYHAGLDWKQFDFEKGFDALMLIGDPEYIFYAGTH
                     WTVFNYMVATDKLIFIGNCEYIYKAGYQWKWFDYYKGWKIMESKIVEGRLWRGKALQT
                     "
BASE COUNT         1345 a          657 c          910 g         1287 t
ORIGIN      
        1 ctccatacta gttattatgt caaaatacta tttaaagagt tttaattaac aggatagtgc
       61 tatcgggaga atgacagtgc gttgttatat gcaaataatt tgtttactgt atatcgtcat
      121 actctgtcat gtcaatgtgt atgctaacta tgagcaaaaa ggaagtgttg gtggtgagcc
      181 tttacaggat gtatttgtca taagtagaaa agattggctg attgtaccat atgtaaatta
      241 taaacccgaa acacagctgg ctggtggtgc tatattcctt acctattttc aggatatcgt
      301 tatgaatata cctcctataa aattaagacc aacaacattg caagtttcag ttacacaaac
      361 acaaaatggt cagtttatgt ttcaggtatt accggaattt tatttttacc gggataggtt
      421 ccatatcaaa aatgatttcc agtatatgga ttattatgat aaattttatg ggataggtca
      481 ggatacctcg gataagcata atgaatatta ccgttcaagg gtatttgcat tcaagaatgt
      541 tgctgaatat gctcttgttc ctaaattata tatgggatta atctatcata ttgatgtcag
      601 ggaaaccaca aaagctgaaa gtggcggttt aattgatgca aacctgctgc ccggcgcaaa
      661 agagggattg gtgtcagggc tgggatttac tgtgaattat gataatcgtg atgatattat
      721 ctatacccga agtgggagct atgcaaattt aagtgtaata cagtattccg aatatataaa
      781 aagtgatttt aattatacca acattacctt tgattttcgt tattttgagc catttttgtt
      841 ttcacatata ctggcttttc agttttatac tggatatatt gatggagaag caccatttta
      901 cctgctttca atgctgggtg gcgataaact tatgcgcggt ttgtaccagg gcagatacag
      961 ggacaatgat gtaatggttg cacaggttga ataccgggtg ccacttttct ggagattcat
     1021 tgcctgtgga tttgcaggag caggagaggt tgccccgggg atggatgatt ttaaagtaga
     1081 tgagatagtg tatgcatggg gcggtggatt gcgatttaaa ctaagggcaa accaggattt
     1141 aaatgtacgg atggatgttg ggtttagtaa aaattttatt ggtatttatg caacgatagg
     1201 agaagcaatt taaaatagaa aggattctga cattatacta tcataattga aagttaaaaa
     1261 gcagattctg agtcaagccc ggaatgacaa tgtgtggagt ctggaatgtt gaacgtcatc
     1321 atgaactggt ttcaggagct aacgctgcat attttgattc cggaacatgt cctgaatgac
     1381 gatatggtga catgtcattc tgaagcaaag ccgaagaatc cggtcgttca aaggagatgc
     1441 ttcggtcact aacgcttttt cagcatgacg cagtactatc ccatttttga aagctcacag
     1501 aataaaatgt tgaataatca tcatttcaac gatacaatga atatcatagg ttgctatcga
     1561 ttaaattatt atctttttca attattatga tgtgctatga aacgaccggt agaagtatca
     1621 acaggtgaat taatcgagat tctggatcga tatcttcaga ctcaggggaa ggtccactat
     1681 gccattaaaa tagttggata tcccggagtt ggaaaatcag ctatagtcca gcagattgca
     1741 cataagcata attatttatt tattgatact cgtttagcat ttaaagaaaa tgttgatctt
     1801 ggtggttacc ccgtaccgga taacaaccaa caacgtatgg tatatttcag acctcgtttt
     1861 ataccaccgg aaaaagtacc tgaaggctat aatggaattt tgtggttttt agatgaagcc
     1921 aacagggctc atccaacggt aatacaaaca ttattccaga ttatcaccga gcgtatgtgc
     1981 ggtgagcacg cattacccga taaaacagtt atagtattgg caggcaattt gggcgatttg
     2041 gacaataccg taattactga ttttgatgat tcagccctgg atggaagatt agcagtattt
     2101 aatcttatcc ctgatgcaca aaactggttg cattgggcac tggaagaagg aatacacccc
     2161 gcagtagtac ggtatattag cacctatcct gaacggttgt gggatgaaag gggtgttaat
     2221 cctaatccac gtggatggca tcagatatcc gacacattat ataaagccta taacttccgc
     2281 actgaagaac aattggtgta ttatttacag catactgact ataaggtttt gcaaaaaatt
     2341 attatttcat taattggcga ggttgcggga actgatttta ttttacaata tgttgcgcct
     2401 cgcgttataa gctcgcagga aatattggca ggtgatgagc aaaaattaca aaaactgatt
     2461 gcaggtgata tacctgctga agacatgatg tgggcactta acggagcgct caataagctt
     2521 tataatgaca atctgcatag caatggaaag cttggtgaag atatacaaaa agaacttttc
     2581 catgtattac agtttattgc atatacgcgc gctgatatac gggtatcgtt catgtatgcg
     2641 cttgttcgtg attgcggcat ctttacgcag atacacgatg cgttgaactt tgaagatgat
     2701 cccgtcaaaa aaaaggattt attagaaaaa attcaggttt tccttgatgt ttagcgatgg
     2761 ttcaggattc atatatacat accactcttc tgcaactatg gaatcatagc aggtttacat
     2821 cgtattttta tcaaaccgtt gaatttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnaaa cggactatta aaaataaggt tattgcacat gcaaaaaagg atgagtggtg
     3001 caccagtgag cgctcatata tggacatcat atcatgtata gaaaaaataa atcttagcga
     3061 tagttctcat tacagccagc acattaaaag tatcatcgat tacatgtcac attcaaatga
     3121 gtggtattat acccattcgc gatttaacag acgctatgtt gctaaaggca tatataatgc
     3181 agggagagca tataagcaaa atgagataat aacagtggct atcgacattt cagcttccat
     3241 gatgtcgcag aaggacgaca tagaaacagc atttggcata gttgatgatc taaccggaaa
     3301 atataaggtg tatcttgttt gtgtcgatga agaatatttt ataccggtaa aagatggtga
     3361 tgcactgata cgggcaaaca ggttaaccaa accgtatgaa tataaaaagg gcgattggaa
     3421 gctgataaaa tcaggctatg gcggggcaac atttttttcg tcgtttttca atgaatattt
     3481 acggcaccat aacgagatgg tacttgtcat tacagatggc tacatctatg atattgacaa
     3541 attaaaacca tacccagcta cactatggct tgttgcaaaa agcaggaatg aggcttttgt
     3601 tccaccattt gggaaagcta taccaatggt ttaggttgct actatggatg agcacataaa
     3661 tactaattat aaccgggcac agtcactata tcgcgaaggt attgaaaata ccgctcattc
     3721 tttgcaatat tttgacgatt tactcaactg taatatttca gaatttatca ttaaagcagg
     3781 cagggactgg aaaaattttg atgttgatgc agccatggat ttttttataa gcaataatga
     3841 tatcgaagcc ttctatcatg cagggatata ctggaaaaat tttaattatg aacggggcat
     3901 cactgtcata gtggaatggg ggaatgatga atatattttc agagccggaa gattctggaa
     3961 gcaatttgat tataacaggg gattgtcaaa actgatagag ttgcaatctg caaaatacat
     4021 ataccatgca ggattggatt ggaaacagtt tgattttgaa aagggctttg atgcattgat
     4081 gcttattggc gatcctgaat atatttttta tgcaggtact cactggacag tatttaatta
     4141 tatggttgca accgataaac taatttttat tggcaattgt gaatatatct ataaagcggg
     4201 atatcaatgg aaatggtttg attattataa aggctggaaa attatggaaa gcaagattgt
     4261 tgaagggaga ttatggcggg gtaaggcatt gcaaactga
//
LOCUS       DAOOJH010000152         4285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16090_length_4285_cov_7.604492, whole genome shotgun sequence.
ACCESSION   DAOOJH010000152 DAOOJH010000000
VERSION     DAOOJH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16090_length_4285_cov_7.604492"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLJ75_12075"
     CDS             <1..715
                     /locus_tag="PLJ75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPD05993.1"
                     /translation="ITIIGNYLNACKPDVSILEIGTGMGIAAACLKKAGFDVHAIEPH
                     TAGFENRSLLYQRVYALLDIGDIVLPVTIEQMPLNKQYDFIFSFNVLEHVNRIEAAFR
                     VLSHILKPAGVMVHSCPNYYIPYEPHCNVLLLPFVPQYTHYICRRLRNSVGLSLTFTT
                     VGLIKKLAKTNNLAVTFKNDVLYNAFRRFEYDAAFAQRQKGLPLVVYKLLKKCRILWL
                     AKHVPVVMQTPMVFELKKK"
     gene            complement(717..2861)
                     /locus_tag="PLJ75_12080"
     CDS             complement(717..2861)
                     /locus_tag="PLJ75_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G-protein coupled receptor"
                     /protein_id="HPD05994.1"
                     /translation="MKIAKIVVTVILLLFIAAALFIGNTRPIDDGDTWWHMEYGQYML
                     EHKTLIPHHSLYTWTPASNDTVYCAWIPEIILYALYSAYGFYSLYAVKYALAVFALAL
                     FILYAIKRNVLTHPVSLFVILMGVITHGNSSGSMIKPELFTYVFMTIMVFLWYMFSLT
                     MQKNLAYLYGIPIVILIWVNSHGGFIFAAPFFVCTYIGILLNYKFQKDTTVNKKVLRH
                     ITIAWILSFLATFVTPYGYSYHLDLLQSLLLKPDVENLKTVQAYTPTLAVPTFVLYFV
                     IPIIILLYTIISTKRYRLHTIDWVFIVNNVMYAALYTIFGRLIYYWIPVYVFSTLFYI
                     KGDAWYYTPKKRWIPAIITAIMLLAGLDQLRWHTFINFTANATWFGFGNSFINSDEEA
                     NFIAKYYPGYTIGNPYNCGGYFLWRMYPDNKIMIDPRYFPFKSWYNEYLKFASRQDVE
                     EFMSKYPFDILHLQYMDLHLYPYFLYHPDWKVAFYGTGGIVFVKKHIPLPGNTFQYSN
                     YIYSYPSLTQSVLILNFLARTLNWQGCELLLQHMQQEFTWSNQKPLVEAANTMYQGVR
                     AFYRRDYAKANELFEKTKGRAPIPANILSTLYHYKAVDLWTQNKIYDALDYTTRVLEL
                     NNYDEPYATFNIGVINWYIYKQYQQQGITGFITQTPVQKNWMEPLQQFLKQTAGNPKA
                     KPARDIANAIINGTYSGDKPPLLYPPEPAYMP"
     gene            complement(3217..3513)
                     /locus_tag="PLJ75_12085"
     CDS             complement(3217..3513)
                     /locus_tag="PLJ75_12085"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD05995.1"
                     /translation="MDQTIVAKAKQYASIVRRTMPVKRIVLYGSYAKNSENSSSDIDI
                     AVIVDKFNGDYLELSAKLFELVQKVDVRIEPILLNENSDTSGFIESILKYGKEI"
     gene            complement(3468..3848)
                     /locus_tag="PLJ75_12090"
     CDS             complement(3468..3848)
                     /locus_tag="PLJ75_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPD05996.1"
                     /translation="MAVYDLDTARAMHETGRYLYVGFMCHQVIEKSLKAYYWYSIHNE
                     PPYTHNLLLLAEQSKLKQYMNEDMNTLLNYLMPLNIQARYPQDKNELIKALTEQVCND
                     LLKKTEELFEWIKQLLQKQSSMPA"
BASE COUNT         1228 a          805 c          875 g         1377 t
ORIGIN      
        1 tattactatc ataggcaact atctcaatgc atgcaagccc gatgtttcaa tactggagat
       61 tggcaccggc atggggattg ctgctgcctg tcttaaaaag gcaggttttg atgtgcatgc
      121 cattgaaccg cataccgcag gctttgaaaa tcgttccctg ctgtatcaaa gggtgtatgc
      181 tcttttagac ataggtgata ttgttttgcc ggtaactatc gaacagatgc ctttgaacaa
      241 acaatacgat tttatttttt catttaatgt gcttgaacat gttaacagga ttgaagctgc
      301 tttcagggta ctatcgcaca tactaaaacc cgctggcgtt atggttcata gctgtcccaa
      361 ctattacata ccgtatgaac cgcattgcaa tgtgctgctg ctgccttttg tgccgcagta
      421 tacacactat atttgcagac gcctgcgcaa tagtgtgggg ctatcgttaa catttaccac
      481 ggtggggtta ataaaaaagc ttgccaaaac caataacctt gcggtaacct ttaaaaacga
      541 tgtgctgtac aatgcgttta ggcgttttga gtatgatgca gcgtttgcac aacgtcaaaa
      601 ggggctgccg ctggtggtgt ataaattgtt aaaaaaatgc aggatactgt ggctggcaaa
      661 gcatgtacct gttgttatgc aaacacccat ggtatttgag ctgaaaaaaa agtaggctat
      721 ggcatatacg caggctcagg ggggtacaaa agcggcggct tatcaccgct ataggtgccg
      781 tttatgatgg cgttggcaat atcgcgggcg ggctttgcct ttgggttgcc ggcggtttgt
      841 tttaaaaatt gctgtaacgg ctccatccag tttttttgta ccggtgtttg tgtaatgaag
      901 ccggtaatgc cctgctgttg atattgtttg tagatgtacc agtttatgac gcctatatta
      961 aaggttgcgt agggctcatc gtaattgttt agctccagca cccttgtggt atagtcaagg
     1021 gcgtcgtaaa ttttgttttg cgtccataaa tcaaccgctt tatagtggta cagtgtactt
     1081 aaaatattgg cggggattgg tgctctgcct tttgtctttt caaaaagctc attggctttt
     1141 gcatagtcgc ggcggtaaaa ggctctgacc ccctgataca tggtgtttgc cgcctcaacc
     1201 agcggctttt gattggacca tgtaaattct tgctgcatat gctgtaacaa aagctcgcaa
     1261 ccttgccagt ttaatgttcg cgctaaaaag tttagtatca gtactgattg cgttaagctg
     1321 ggatagctgt agatgtaatt gctgtattgg aaggtattgc cgggaagtgg tatatgcttt
     1381 tttacaaata ctatcccgcc cgttccgtaa aatgccacct tccaatcggg atgataaagg
     1441 aaataagggt atagatgtaa atccatatac tgaagatgca gtatatcaaa ggggtatttg
     1501 ctcatgaact cttcaacatc ctgccgggat gcaaatttaa ggtattcatt gtaccagctt
     1561 ttaaacggga agtagcgcgg gtcaatcatg atcttattgt ccgggtacat gcgccacaaa
     1621 aagtagcctc cgcagttgta gggattgcca atggtatagc ccggatagta ctttgctata
     1681 aaatttgctt cttcatcgct gttaataaat gaattgccaa atccaaacca tgttgcattg
     1741 gcggtaaagt tgataaaggt atgccagcgc aactggtcca accccgctaa cagcatgatg
     1801 gcggtaatga tggctggtat ccaccttttt tttggtgtat agtaccacgc atcccctttt
     1861 atataaaaca gcgtgctgaa tacatacacc ggtatccagt agtagataag ccttccaaat
     1921 atggtgtata aagccgcata catcacattg tttacaataa acacccagtc aattgtatgt
     1981 aaacggtagc gtttggtgct tataatggta tatagcagta taataatggg gataacaaaa
     2041 tacagcacaa aggttggtac ggcaagggta ggtgtataag cctgcactgt tttaaggttt
     2101 tcaacatcag gctttagtaa aagcgactgc agcaaatcaa ggtgatagct gtatccatac
     2161 ggtgttacaa atgttgccaa aaatgacaat atccatgcaa tggtaatatg ccgtaatact
     2221 tttttattga ccgttgtatc cttttgaaat ttataattta agagtatgcc tatataggtg
     2281 cacacaaaaa atggtgcagc aaagataaaa ccgccgtggc tatttaccca tataaggatg
     2341 acaatcggaa tgccataaag gtatgcaagg tttttttgca tggtaaggct aaacatgtac
     2401 cataaaaata ccatgatggt cataaacaca taggtaaaaa gctccggctt tatcatactt
     2461 cccgacgagt tgccgtgcgt tataaccccc attaaaataa caaacaggct tacaggatgg
     2521 gttagcacat ttcttttgat tgcatacagt ataaacagcg caagcgcaaa aacagccagt
     2581 gcgtatttta ctgcatacag gctgtaaaat ccatatgctg agtacagtgc atagagtata
     2641 atctcaggta tccatgcgca gtacaccgtg tcgttgcttg ccggtgtcca tgtgtacagt
     2701 gagtggtgtg ggattagcgt tttatgttcc agcatgtatt gtccatactc catgtgccac
     2761 catgtgtcgc catcgtcaat ggggcgggtg ttaccaatga ataaagcagc agcaataaac
     2821 agcaacagta tgacggttac tactatcttt gcaattttca tgatgtgtac cgtattaaca
     2881 tggattttta tgccggtgaa aatgatagta tatgccgggt atactttttt ttagcaagca
     2941 ttttattgtt ctttcttttt ttttgggcga tagttaccgg gtagatagta tcataaatga
     3001 tgtaatgtac agtaactatt ggctatttgt taaaaaaagt atataaagta tataaagtat
     3061 atattgaatc aattactggt tttaattcat catttgtcat tccgcaatat agcggcagtc
     3121 gtaataatcg ttgactttct tgtgttgtga actggtcaat ctactataat gtgaaaactt
     3181 tatcatcttt ttcttattgc ttcaaatttg actttatcaa atctctttcc catatttcag
     3241 aatgctttca ataaaaccac ttgtatcaga attttcatta agcagaatgg gttcaatgcg
     3301 cacatcaacc ttttgtacta attcaaaaag ttttgcactt agttccagat aatcaccatt
     3361 aaacttatca acgataactg ctatatcaat atcgctgcta ctgttttcag aattttttgc
     3421 gtatgatcca tacaaaacaa ttcttttcac aggcattgtt cgtctaacta tgctggcata
     3481 ctgctttgct tttgcaacaa ttgtttgatc cattcaaata actcctcggt ttttttgagt
     3541 agatcattac aaacctgctc agtgagtgct tttattaatt cgtttttgtc ctgtggatag
     3601 cgtgcctgaa tattcaaagg cattaaataa tttaaaagcg tgttcatatc ttcattcata
     3661 tactgtttca attttgactg ttcagcaagc agtagaagat tatgagtata tgggggctca
     3721 ttatgaattg aataccagta atatgctttt agtgattttt caattacctg atgacacatg
     3781 aaaccaacat aaaggtacct cccggtttca tgcattgccc ttgcagtatc aagatcataa
     3841 actgccatat caagccaata attaattttg ttttgaatat ccactgtttt aattttatat
     3901 aaaatattat tttatgtcaa gcagattaca ataggtgtta attaatacaa tggaatgcta
     3961 tagttactat gcaaacaaag ttttccctta atgaactttg agtcatcatt agctatttgt
     4021 taaaaaaagt atatactgca tttactccat tgttatttaa gtattcaatc aatgcgctgc
     4081 gcgggtttat atcccttact gagttattaa ggctttgcgt atggcatcta ttttccaaca
     4141 aaaagaccaa acacagcaat aagtatgctg atgacggtaa agccaatgcc tataaaccac
     4201 tgcagggtgg tgaaacgttt ttccattgcc tcaaagcgag tttccattgc ttcaaagcgt
     4261 gctttttcta tctcttttaa tgctt
//
LOCUS       DAOOJH010000153         4251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16248_length_4251_cov_9.503813, whole genome shotgun sequence.
ACCESSION   DAOOJH010000153 DAOOJH010000000
VERSION     DAOOJH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16248_length_4251_cov_9.503813"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..739)
                     /locus_tag="PLJ75_12095"
     CDS             complement(263..739)
                     /locus_tag="PLJ75_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD05997.1"
                     /translation="MAKKPLNFQQSIIDFMRSKSDRMERELRVPGDWYFNDGDEQEIK
                     SWTDEEAEKVWEKIKHNIYKLGCSGLRYELCPFCHYYGYEHNGCYKALKNPICVKCNY
                     GKRHGICIGEEGQVSEYKKILQSFEESRISMYKFFTNEYYTELIEKIEKNNAKAIA"
     gene            complement(931..1377)
                     /locus_tag="PLJ75_12100"
     CDS             complement(931..1377)
                     /locus_tag="PLJ75_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPD05998.1"
                     /translation="MQCDKLIVGIGNRLRSDDAAGSIVARRLAQAGFCSIDTESMPEN
                     YIGTIQRRKPKTLVIVDACDMNLPAGEVRRIDLSALYGGIITTHGLPVHILLQKLQEV
                     TPHITFIGIQPETIAIGDIVNVHVHHGIDYCVTMLVNDKETDIPLL"
     gene            1514..2713
                     /locus_tag="PLJ75_12105"
     CDS             1514..2713
                     /locus_tag="PLJ75_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD05999.1"
                     /translation="MISKGLIERIFQAASIQRWNDHVRPMEFAEIDKQAHKMILAFTI
                     AKFQEEEKHGCVNWRLLIEGGIIELLHRVIVTDIKPPIFHKIMKEKGRELNEWVFEQL
                     QDDIGSVKGNFRETFQRYLFDDNFAVFEKKILHASHYLATNWEFQIIYHSNTFLYDIE
                     KTRNEIESQIEYHIDLTGVQRILLHSDIARFISLCGQLRFQQRWAQTHRIPKTSVLGH
                     MLIVAILSYFCSCELNTCERRMYNNFFGSLFHDLPEVLTRDIVSPIKRSIKGLDQLIK
                     EYELNEVNERIFPLIPQNWHDEMKYFIFHEFENKVYIDNSITMGISFDAMNTQYNHDI
                     YNPMDGEILKACDDFAAFLEASFSINYGIKPEGLVSARRSIYQKYKAKHLVIGDVDFI
                     ALFDYFY"
     gene            2738..3133
                     /locus_tag="PLJ75_12110"
     CDS             2738..3133
                     /locus_tag="PLJ75_12110"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HPD06000.1"
                     /translation="MPRTIKNLLLNYRDSIGALLVALKHPHVPMHSKMLIIIALAYAL
                     SPIDIIPDFIPILGYLDDAVIVPLLFYVAIHSIPQPVFYQCKQQAKNTFKKSIGCMVL
                     GLLLAALFWGFMALLLFILIKTILRIVAV"
     gene            3130..3831
                     /locus_tag="PLJ75_12115"
     CDS             3130..3831
                     /locus_tag="PLJ75_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06001.1"
                     /translation="MIAIINPDTPKPIVEGLMSHGFTPVTIPLCTTVQRPIAGHPDIQ
                     LCMIHNFVIYNPAIEQFISKLLHQSQYIPIRGQSIVQPPYPYDCAYNCVCTGIVAFHN
                     TKATDTAIQEILHRCSVPLIHVKQGYSKCSTCIVNTHAIITPDKTIHRAAPKNNITSL
                     LIQPGTIILPGYPYGFFGGASGLCHDTVYVTGTLSKHPDTAKIEDFIRNHGKNIIYLS
                     NLPAIDIGSIFFLDL"
BASE COUNT         1276 a          874 c          726 g         1375 t
ORIGIN      
        1 atgaaatcaa atacctactg catagataac agatgctcat actggaggaa tactgaacat
       61 atttactccg catttgttag caaaaatgaa gaattgggca aacatctttt tagttttaaa
      121 aattttattg cccttgagta atagtataaa gccatcaacc atattaacct ctgatacatt
      181 atacgcaaaa aataaagaga cggacatgta taccgtctct ttatttttca taaaaatgtt
      241 gtaatatact cagtactata aatcaggcaa tagcttttgc gttattcttc tctatctttt
      301 caattaattc agtataatat tcattagtaa aaaacttata catgctgatt cgagattctt
      361 caaaggactg taagatcttc ttatattcgc ttacctgccc ttcctcgcca atacatattc
      421 catggcgctt gccatagttg catttgacac atattggatt ttttaatgcc ttatagcacc
      481 cattatgttc atacccataa taatggcaaa aaggacaaag ttcatagcgc aatcccgagc
      541 aacctaattt atagatatta tgtttaatct tttcccacac tttttccgct tcttcatcag
      601 tccagctttt tatttcttgt tcatcgccat cattgaaata ccagtcaccc ggtaccctga
      661 gctcccgttc catcctatca gacttggatc gcataaaatc gataatagat tgctgaaaat
      721 ttaatggttt ttttgccata acaatatccc tttaattata ttttaattat tacatatacc
      781 tttcacttca gactatcgac cagatttatc attccaaact tcattttaac ataatttaaa
      841 ttaatatata tggaatccac ttcctgttta ataaagtata cccattcggt attttttcaa
      901 gctttttatt accattatta caattatttt tcataacaaa gggatatcag tttccttatc
      961 attgacaagc attgttacgc aatagtctat accatggtgc acatgaacat tcactatatc
     1021 tcctatggca atagtttcag gctggattcc aataaaagta atatgcggtg ttacttcctg
     1081 taacttttgt agcaaaatat gcaccggcaa cccatgggtg gtaataattc caccatataa
     1141 tgctgataaa tcaatccgcc gcacctcgcc tgccggtaaa ttcatatcac aggcatcaac
     1201 aataactaat gtttttggtt tacgccgttg aattgtgcca atataatttt cgggcatact
     1261 ttctgtatca atactgcaaa aaccggcctg tgccaatctc cgggcaacga tactccctgc
     1321 ggcgtcatcg ctccgcaaac gattcccaat accaactatg agcttatcac actgcataat
     1381 ccattaccgg taactatcgc caaaaatttt tgtatttgtg ccactatcaa caatcacctt
     1441 taaatatttt acttgcattt accagcaatt caagttttat tttgctatgc gatagttacg
     1501 ggagatataa tctatgattt caaaaggttt aattgaaagg atatttcaag ctgccagcat
     1561 ccagcgctgg aatgaccatg tgcgccccat ggagtttgcc gagattgaca aacaggcaca
     1621 taagatgata cttgccttta ccattgcaaa gtttcaggaa gaagaaaaac atggctgtgt
     1681 taactggcga ttgctcatcg aaggaggcat tattgagctt ttacacaggg tgatagttac
     1741 cgacataaaa ccgcccattt ttcataaaat aatgaaggaa aaaggcaggg agttaaatga
     1801 atgggttttt gaacagcttc aggatgatat tggttcggtt aaaggtaatt tcagggaaac
     1861 attccaacgc tacctgtttg atgacaactt tgcagtattt gaaaaaaaga ttttgcacgc
     1921 ttcccattac cttgccacta actgggagtt tcagattatc taccacagca atacattttt
     1981 gtatgacatt gaaaaaacac gaaacgagat agaaagtcag attgagtacc acattgacct
     2041 cacaggtgtc cagaggattt tgctccacag cgatatcgca cgatttataa gcctgtgcgg
     2101 gcaactgcgt tttcagcagc gctgggcaca gactcaccgc atccctaaaa catcggtgct
     2161 ggggcatatg cttattgttg caatactgtc gtacttttgc tcgtgcgagc ttaacacctg
     2221 cgaacgtcgt atgtataata acttttttgg atcgctcttc cacgacctgc ccgaggtgct
     2281 cacgcgcgat attgtttcac ccattaagcg ttccataaag gggctggatc aactgataaa
     2341 ggaatatgag cttaacgagg tcaatgaacg tattttccca ttaataccgc aaaactggca
     2401 tgatgagatg aaatacttta tctttcatga atttgaaaac aaggtgtaca tcgacaactc
     2461 cataaccatg gggattagct ttgatgcaat gaacacacag tacaaccatg atatatataa
     2521 tcctatggat ggtgaaatac tcaaagcctg tgatgacttt gcagcatttt tagaggcttc
     2581 attttccatt aactatggaa ttaaacccga agggcttgta tctgctcgac gcagtattta
     2641 ccaaaaatat aaagcaaaac atcttgttat tggcgatgtt gatttcatag cactgtttga
     2701 ttatttttat tagacaatta ctttttttcg caaatgtatg ccaaggacta ttaaaaacct
     2761 gttattaaat tatcgcgatt caatcggtgc attgcttgtt gctctgaagc atccccatgt
     2821 tcccatgcat tcaaagatgc tcataattat tgcactggca tatgcactgt ctccaattga
     2881 tataataccc gatttcattc ccatacttgg ctatttagac gatgctgtga ttgtgccact
     2941 gctgttttat gtagccattc attcaatccc gcagcctgtg ttttaccagt gtaaacaaca
     3001 ggcaaaaaac acatttaaaa aaagcattgg atgtatggta ttgggcttat tgcttgctgc
     3061 attgttctgg ggtttcatgg cactgcttct ctttatactt ataaaaacaa tactacgcat
     3121 tgttgcggta tgatagcaat tattaatcct gatacgccca aaccaattgt tgaagggctt
     3181 atgagccatg gatttactcc ggtaaccatt ccgctatgta ccacggtaca gcgtcctatt
     3241 gccggtcatc ctgatattca attgtgcatg attcataatt ttgttatcta caatcctgct
     3301 attgaacagt ttattagcaa actgctgcac caatcacagt atattccaat acggggtcag
     3361 agcattgttc agccacccta tccatatgac tgtgcataca actgcgtttg taccggtata
     3421 gtagcttttc ataacacaaa agcaactgat actgcaatac aggagatttt acaccgctgc
     3481 tctgtccccc tgattcatgt taagcaaggg tattcaaaat gctcaacctg cattgtcaat
     3541 actcatgcta tcatcacacc cgataaaacc atccaccgtg ctgctcccaa aaacaatatt
     3601 acctcattac tgatacaacc tggcacgatt atacttccgg gatatccata cggttttttt
     3661 ggaggtgcaa gcgggctatg ccatgacacc gtttatgtta ctggcaccct ttcaaagcat
     3721 cctgatacag caaaaataga agattttata cgaaatcatg gtaaaaacat tatctatctg
     3781 tcaaatctgc ccgccattga tattggttct atcttttttt tagacttata atgcattgtt
     3841 gtatggttta ataggggtca gagccttaat cattcaattg caaaaaattt gaaagttacc
     3901 cgggcatgga tgtatattgt attaatggca ttgtagagca ttattatata gttgtaccca
     3961 tgtgttgcaa tgggagtgta tattgcatgt actgcgtgct tttcatgatg ctatatcagt
     4021 ttatacgtgt tgcacgatat gcagaagcac aatcaatgga agtaataagt atatttttta
     4081 aatttcattg atctggttgt taccctgcag tatcaaacat aatgtttaag atgtttcttt
     4141 actttatatt gttaaatcta tgcgacaggg ctatgaagat tcctgatatc aatgagtgct
     4201 taaagctttt ggaactttat aatacattac ccaatgtact gcagcattct t
//
LOCUS       DAOOJH010000154         4197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16516_length_4197_cov_8.387253, whole genome shotgun sequence.
ACCESSION   DAOOJH010000154 DAOOJH010000000
VERSION     DAOOJH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16516_length_4197_cov_8.387253"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..654
                     /locus_tag="PLJ75_12120"
     CDS             478..654
                     /locus_tag="PLJ75_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06002.1"
                     /translation="MPDSGPYLPLKKGGIKSKKSAGLVFNAFTQKHLQLGLKVIEKIQ
                     EKSNEGVMQLRSEW"
     gene            681..1148
                     /locus_tag="PLJ75_12125"
     CDS             681..1148
                     /locus_tag="PLJ75_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06003.1"
                     /translation="MNLETTTCISYEHLDVIMHHSEKYDMSMRSFIVSFINFVATHKI
                     VPNKPYSRLTYRSRGIAEWKRLHLVLLENEYEFFLDVKKLWKMSLAKIIAFCIENYLY
                     DFIEALENTDNTDNYRFSGYSFGVFLEEGIQCCQFYWGPPPSILKKTKNTLQQ"
     gene            complement(1196..>2734)
                     /locus_tag="PLJ75_12130"
     CDS             complement(1196..>2734)
                     /locus_tag="PLJ75_12130"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPD06004.1"
                     /translation="NQIVEGKLNVTGVAIPGQPCIICGHNEYIAWGMTNVMVDDMDFY
                     LEKINPANKYQYMLNGKWKDMIVVKEEIKIKGEKPVIQEILFTHRGPVVTDFKKMQDV
                     ISMRWMGNEPSNELLTVYMLNYAKNWNDFTNAIRHFRSVSQNIAYADVEGNIGLYSAV
                     GIPIRKGNPMMINPGWTTEYDWQGLVPFERQPHSFNPEEGYIASANNKTVKENYPYYI
                     SYWYEMPWRYNRITKLLKQKEKLSVEDFKTIQTDQISELALIWKPLLLHAIERIIEGD
                     SNSLNSYKDVYTLLKDWDGSYSSESIAASVFDTFYIKLVHNIFYDELGDTYDELAKER
                     VLVNLFIDTIRLNNGSIWCDDVTTPQKENLNDMIAKSLNDTIGTLTDSYGTNINKWKW
                     GSMHKLVLKHPLGEVKLLDWLFNYNRGPYPVGGSFHTVCPYSYKLTKPFVITDGASER
                     HIFPMHNLNESLTVIPTGTSGNVASPYYCDQTSLYVGMHYHNDYIDSELIKRTGKYLL
                     QLTP"
     assembly_gap    2735..2834
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2835..3439)
                     /locus_tag="PLJ75_12135"
     CDS             complement(<2835..3439)
                     /locus_tag="PLJ75_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPD06005.1"
                     /translation="MSKTKKILIGIAIVIVALVLAIFLFIKNLAKSGAPQYSGQIQLN
                     GLQQPVQVYRDEHGIPHIIAKNEMDVYRATGFVMAQDRMWQMDLLRRVTSGRLSEIFG
                     KDMVDTDVLMRALRMPQKSRFVIAQTDKKVIAHLEAFADGVNQYLEQHKNNLPLEFKI
                     LGYTPESWKVEDSVNLIGYMAWDLTMPWTTETVLYKIWKKV"
     gene            3592..4149
                     /locus_tag="PLJ75_12140"
     CDS             3592..4149
                     /locus_tag="PLJ75_12140"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPD06006.1"
                     /translation="MDVSFPVNKQSIRLPKINQLGIVVHSIPDAVHYYTTLLGIGPWF
                     RSGTVEHTTLYRGTNISIDVDIVLAFHKGMEVELIELKGGDECIYSECIQRGGGIHHV
                     GSLVLSYDSKMKRVHEAGIQILQSGVIKTRGGATTRYAYLDTVKQCGIITELIETRLY
                     KIPVPQNRLFMEIGCLTGDVGKVYG"
BASE COUNT         1207 a          766 c          808 g         1316 t
ORIGIN      
        1 ggagatcagt tgaaagacat gctggtggca ccgtcatggg gtttgcataa agggacagtc
       61 cttaaagcaa ttgaggatga attcaaaagg cgtggtactt accagggttt tttgtacgat
      121 agttactgaa aattttgtga ttgcattata aacaatattc gcctatgata gtaacaataa
      181 aacttgtatg ttacttcatg tataattgca aaaactttga tagaggtaac atgatggttg
      241 gtatctattg ttagaatcat gtattcagcg gcaaggtatt tttaccgttt atacaattat
      301 acaataccct gtgattctca cacgataata gcatgaactt acgcccggat accattgctc
      361 gaacagcttt agaacgctat accgattcag caatagatga ttttggatga ttcattttac
      421 tgtgtaattt tcgacgttat gtagacgact ttgcattcat gacgggagcc aaagtaaatg
      481 ccggatagtg gaccgtattt gcccttaaaa aagggtggta ttaaaagcaa aaaaagtgca
      541 ggtttagtgt ttaatgcttt tactcaaaaa catttgcaat tgggtttaaa ggttatagaa
      601 aaaatacagg aaaaaagcaa tgagggagta atgcagttaa ggagcgaatg gtaaaaaatt
      661 atttagtaag ggataatgat atgaatcttg aaacaacgac ttgtatatcc tatgagcatc
      721 tggatgtaat aatgcatcat tctgaaaaat atgacatgtc aatgaggtca tttatagttt
      781 catttattaa ctttgttgct acacataaaa ttgttcctaa caaaccttat tcccgattaa
      841 catatagatc aagagggatt gctgaatgga aacgtttaca tttagtatta ttagaaaatg
      901 agtatgaatt ttttcttgat gttaagaagc tctggaagat gtcactggct aagataatag
      961 ctttttgcat agagaactat ctctatgatt ttatagaagc tttagaaaat actgataata
     1021 ccgataacta tcgttttagc ggttattctt ttggggtatt tcttgaagaa ggtatacaat
     1081 gttgtcaatt ttattgggga ccaccaccat caatactcaa aaaaacaaaa aataccttac
     1141 aacaataatc cagacagtat caatgcatac cgtctggatt attgctacaa taaccctatg
     1201 gggtaagctg taatagatat ttaccggtac gttttatcaa ctcactgtca atataatcgt
     1261 tatgatagtg catcccaaca tataatgatg tttgatcgca atagtaggga cttgctacat
     1321 ttccactggt acctgttggt atgaccgtaa gcgattcatt gagattgtgc attggaaaaa
     1381 tatgacgctc cgaagcacca tcggtaatga caaacggttt ggtgagctta tatgagtatg
     1441 gacaaacggt gtggaagcta cctccaacag gatatggccc gcgattatag ttgaataacc
     1501 agtctaacag ttttacctcg cctaatggat gttttaacac taacttatgc atactgcccc
     1561 atttccactt gttgatgtta gttccatagc tatcagttag tgtgcctata gtgtcattca
     1621 ggctttttgc aatcatatcg ttaaggtttt ctttttgcgg tgtggtaaca tcgtcgcacc
     1681 atatagaacc gttgttaagc cgtatggtat caataaaaag gtttaccagt accctctctt
     1741 ttgcaagctc gtcataggtg tcgcccagct catcgtaaaa gatattgtgc accagcttta
     1801 tgtagaatgt atcaaaaact gaagccgcaa tgctttcaga agaataactg ccgtcccaat
     1861 ctttcaacag cgtgtataca tctttataac tgtttaacga gttgctatcg ccctcaataa
     1921 ttctttcaat agcatgtaac agtaaaggtt tccatatcag ggctaattcg gatatctggt
     1981 cggtttgaat ggttttaaaa tcttcaaccg acaacttttc cttttgtttt aaaagctttg
     2041 ttatacggtt gtagcgccat ggcatttcat accagtaact gatataatag gggtagtttt
     2101 ccttaacggt tttattattt gccgatgcta tatatccttc ttctggatta aagctatgag
     2161 gttgccgttc aaatggtacc agtccctgcc aatcatactc agtggtccag cctggattta
     2221 tcatcatggg attgcccttc ctgataggga tgcccacggc tgaatataat ccaatgtttc
     2281 cttcaacatc ggcataggca atgttctggc tgacagaacg gaagtggcgt attgcattgg
     2341 taaagtcatt ccagttcttt gcatagttaa gcatatacac ggttaacagt tcattgcttg
     2401 gttcattccc catccagcgc attgaaatga catcctgcat cttcttaaaa tcggttacaa
     2461 cgggaccgcg gtgagtaaat aaaatttcct gtatgacagg cttctcacct ttgattttta
     2521 tttcttcttt taccactatc atatctttcc atttgccatt cagcatatac tggtacttgt
     2581 tagcggggtt tatcttttca agataaaagt ccatatcatc taccatgaca ttggtcatgc
     2641 cccatgcaat atattcattg tgtccgcata taatgcacgg ctgtcccgga attgcaacac
     2701 ctgttacatt gagtttacct tctactatct ggttnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnntccacc tttttccata tcttatacag tactgtttcg gtggtccagg
     2881 gcatggtgag atcccatgcc atatagccta ttaaattaac gctatcctca accttccagc
     2941 tttcgggggt gtatcctaaa attttaaatt caaggggaag gttgttctta tgctgttcaa
     3001 gatattgatt aacgccatca gcaaatgctt ccagatgggc aataaccttt ttgtcagttt
     3061 gtgcaataac aaaccttgat ttttgaggca tgcgtaatgc tcgcataagc acgtcggtat
     3121 caaccatgtc cttgccaaat atttctgaca gtcgtcctga tgtaacacgc ctcagcagat
     3181 ccatctgcca catcctgtcc tgtgccatca caaatcctgt tgcccggtaa acatccattt
     3241 cattttttgc aatgatatgc ggtatgccat gttcatcgcg atacacctgc accggttgct
     3301 gcaacccatt taactgtatc tgccccgaat actggggtgc ccctgatttt gcaagattct
     3361 ttatgaaaag aaatatggct aatactaaag caacaatgac aatagctatg ccaataagta
     3421 tcttttttgt tttgctcata acaaacctcc attatagtgt taatcattct attgaatgaa
     3481 ataataaaag taaagaattt ttttatttgc taaatactgc cacaccgggt attatttgac
     3541 aatagtaacg tctacagggc tcttagcttt ttcattgtcg gtgaggtgta tatggatgtg
     3601 tcatttcctg taaataaaca aagcattcgt cttccaaaaa ttaatcagtt agggatagta
     3661 gtacattcta ttcctgatgc tgttcattac tatactacgt tgctgggtat tggtccatgg
     3721 ttcaggtcag ggacagttga acatacaaca ctgtacaggg gtacgaacat atccattgat
     3781 gttgatattg tgttagcttt tcacaagggt atggaggtag agcttataga gctgaaaggt
     3841 ggcgatgagt gtatttattc tgaatgtata caacgtggcg gtggtataca tcatgtaggc
     3901 tctctggtgc tatcgtatga cagcaaaatg aaacgcgttc acgaagcagg gatacagata
     3961 ctacaatcag gtgtcataaa aaccagggga ggcgccacaa cacggtatgc ttaccttgat
     4021 actgtcaagc agtgcggcat tataaccgaa cttatagaaa caaggttata taaaataccg
     4081 gttccgcaaa acaggctgtt tatggaaatt ggatgcttaa ccggtgatgt gggaaaggtc
     4141 tatggataaa tcaaaaatac tttaatggtg gcgatagtta ttgtttatca atttccc
//
LOCUS       DAOOJH010000155         4178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16603_length_4178_cov_6.747756, whole genome shotgun sequence.
ACCESSION   DAOOJH010000155 DAOOJH010000000
VERSION     DAOOJH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16603_length_4178_cov_6.747756"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLJ75_12145"
     CDS             complement(<1..636)
                     /locus_tag="PLJ75_12145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPD06007.1"
                     /translation="MSEILLVRHGQGSFGQGDYDNLSEKGQKQSLILADYLYNYRYNN
                     KQIDAIYSGTLKRQQHTAQILIDYFLSKGIHVPSLQTDSRLNEYDSINIIKTIFPILL
                     NDDPHLDEEIKQIYTDKKAFQRVFEKVMLYWITTKTSFEGIESYEHVKKRVNSFVTEI
                     TKNHGRGKNIVLVSSGGIIGTTVQMVLGFSDETTMQLTWQIVNTSMTHLRYN"
     gene            850..>1124
                     /locus_tag="PLJ75_12150"
     CDS             850..>1124
                     /locus_tag="PLJ75_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein beta subunit/FixA
                     family protein"
                     /protein_id="HPD06008.1"
                     /translation="MSIPYKILVCIKQVPDANAKIYLEKTKPYYDEKTVYVINRYDEY
                     ALEEALQLKDKGLTHYIHTISAGPDRIQKALRRSLEMGADEAYHIVTG"
     assembly_gap    1125..1224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1225..1769
                     /locus_tag="PLJ75_12155"
     CDS             <1225..1769
                     /locus_tag="PLJ75_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06009.1"
                     /translation="LRRSLEMGADEAYHIVTGDDDCAIPYYVARCITEFAKKEYDIIF
                     TGVMSQDAMNQQTGQLIASIMGYNYATGVTSIQTNDDEITVTRELDAQTSMQYVLKIP
                     CVVTVQSSSTMPRYPSLSNVLRAKKQHIDIIKPIMSSQVQYHSFYTIPQKTTECEFIQ
                     GSTDQKVVELYNILHRNGVV"
     gene            1773..2729
                     /locus_tag="PLJ75_12160"
     CDS             1773..2729
                     /locus_tag="PLJ75_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPD06010.1"
                     /translation="MSILIIADIKNNQINPATAELFTAANIIAKDITTPVYLLIPCND
                     AKITSNYRPDVTTIVIGMGIYHYNAPGLSKAIASIAHDIEAHYIIGLHTPFTIDFLPS
                     VSIEYNAHCVTQVHTIEYDEHLTLARGSYSGKLDQFVSIVDDKPVVASITPGSFDEYG
                     AKDADDLINKVIEINIDTAIKQWCTFIISENKDSSLDEADIIIGVGKGIGSKDNLTLI
                     YNFAKFFPHSAVAGSRIVCDYGWLPYSKQVGITGKSISPKVYIACAISGSSQHIAGIK
                     GAKTIIAINKDPYAPIFNVSHYGIVEDIFTFIPQFLEYIQRL"
     gene            3075..3665
                     /locus_tag="PLJ75_12165"
                     /pseudo
     CDS             3075..3665
                     /locus_tag="PLJ75_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
     gene            3754..>4178
                     /locus_tag="PLJ75_12170"
     CDS             3754..>4178
                     /locus_tag="PLJ75_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06011.1"
                     /translation="MSQPDDFTLDDDLFKLDDEIDIQDNIPENKPEANNNTVLDSGKD
                     YGDSSRKRSSRDFQPDMDALLITAQSSMIIEGMKKYTERDFSSNNLPIYLEALNGVNL
                     YIKILDRNPKNYHKLKSVINADQDCQEVEKIAFDLYKKM"
BASE COUNT         1408 a          646 c          708 g         1316 t
ORIGIN      
        1 gttatatcgt agatgtgtca tcgatgtatt tactatttgc catgttaact gcatggtggt
       61 ttcatcacta aaaccaagaa ccatctgcac agttgttcca atgatgcctc ctgaagaaac
      121 caatacaata tttttccctc tcccatgatt ctttgttatt tcagtgacaa agctgttcac
      181 cctcttttta acatgttcat aggattcaat gccttcaaat gatgttttgg ttgttatcca
      241 ataaagcata actttttcaa aaacgcgctg gaaagctttt ttatcagtat atatttgttt
      301 tatctcttca tcaaggtgcg gatcatcatt aagcaatatg ggaaatatgg tttttataat
      361 attgattgag tcatactcat ttaatcgtga atcagtttgc aatgaaggaa catggatacc
      421 ttttgataaa aaataatcaa taagtatttg tgctgtatgt tgttgtcttt ttaatgttcc
      481 tgaataaatt gcatcaattt gtttattgtt atagcgatag ttatataaat aatcagctaa
      541 aataagggat tgtttttgcc ctttttcaga caaattgtca taatcaccct gtccaaaaga
      601 accctgaccg tgacgcacta aaagtatttc actcataatt caccagatta aaatattttg
      661 aattattaat aacattatca actcctgtat aaaaatgtca agaaaaatat aacgaacgtt
      721 cgttattttt attgattttt ttaaaatatt tttatataat ggtctcaatg ctaagaaata
      781 aaattatagg gagaactgtt tgctatatgt tcaattgttt aatgatgcta tacaggagat
      841 gaagttattg tgagtatacc atacaaaata ttagtgtgca taaaacaagt acccgatgct
      901 aatgctaaga tttacctgga aaaaacaaaa ccgtattatg acgaaaaaac cgtgtatgtt
      961 attaatcgat atgatgaata tgcactggaa gaggctttgc aactaaaaga taaaggatta
     1021 acacattata ttcataccat ttcagcagga ccagatcgaa tacaaaaggc tttgcgtcgc
     1081 tcacttgaaa tgggcgctga tgaggcatac catattgtaa ccggnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnctttgc gtcgctcact tgaaatgggc gctgatgagg
     1261 cataccatat tgtaaccgga gatgatgatt gtgctatacc atattatgtt gcacgatgta
     1321 taactgaatt tgcaaaaaaa gagtatgata ttatttttac tggtgttatg tcccaggatg
     1381 caatgaatca gcaaaccggg caacttattg catctatcat gggatataat tatgctaccg
     1441 gagtaaccag tatacaaact aatgatgatg aaataacagt tactcgtgaa ctggatgctc
     1501 aaacttcgat gcaatatgtt ttaaaaattc cctgtgttgt tacagttcaa tcaagcagta
     1561 ccatgccgcg ttacccttcg ctttcaaatg ttctccgtgc aaagaaacaa cacattgaca
     1621 ttataaaacc cataatgtca tcgcaggttc aatatcatag tttttataca attcctcaaa
     1681 aaactactga atgtgaattt attcaaggaa gcaccgatca aaaggttgtt gaattatata
     1741 acattttaca tcgtaatgga gtggtttaga ttatgagtat acttatcatt gcagatatta
     1801 aaaacaacca gataaatcca gcaactgcag aattatttac cgctgccaat ataatagcaa
     1861 aggatataac tacacctgtc tatttgctta ttccatgcaa tgatgcaaaa attaccagta
     1921 actatcgccc ggatgttaca acaatagtaa ttggcatggg catctatcat tataacgcac
     1981 cagggttaag taaagcaatt gcatcgatag ctcatgacat tgaggcacat tacatcattg
     2041 gattgcatac accgtttacc attgattttt tgccatcagt aagcattgaa tataacgcac
     2101 actgtgtaac gcaggtacac accatagaat atgatgagca tctcacattg gcacgaggaa
     2161 gctactctgg taaattagac cagtttgttt ctatagttga tgataaacct gttgttgcaa
     2221 gtattacacc aggaagcttt gacgagtatg gtgcaaaaga cgctgacgat ttaattaata
     2281 aagtaattga aatcaacata gatactgcta taaaacaatg gtgtacattt ataataagtg
     2341 agaataaaga ttcatcatta gatgaagccg atattattat tggtgtgggg aaaggtattg
     2401 gatcaaagga taatttgaca ttgatttata attttgcaaa atttttccca cacagtgccg
     2461 tagctggttc acgtattgtg tgcgactacg gttggttgcc atatagcaaa caggttggca
     2521 ttacgggaaa aagcatatca ccaaaggttt atattgcatg tgcaatatca ggatcttcgc
     2581 agcatattgc aggaattaaa ggagcaaaaa caataattgc cattaataaa gacccctatg
     2641 ctcccatttt taatgtttca cactatggta ttgttgagga tatttttaca tttataccac
     2701 aatttttaga atatattcaa cgattataaa taatcataat acgcttgaca aaagaataat
     2761 tgaaaatttc taataaaaca attatattgt gtgatagaat aaattacatt tttttcttaa
     2821 tcataaattc atgataaatt aattatgaat tttgaaattg attattacaa tcattacaaa
     2881 tattttcacg ggagatatca tacagtatga aagggaaaat aataacatct ctatttttta
     2941 catttgtagc tataacttcg ctgatttttt ttattattgc tgtcattatc cggattatga
     3001 cctacccttt tgataaaagg ttgcgtttac ttcatctttt tacctgtttc tggggttcac
     3061 tctataccta tgttatgcca ccatggaaaa ttactgtaca gggaagaaat aagatcaaaa
     3121 atagtaaacc gtatatcatt atttccaatc atcagtcaca gcttgacata ttagttgcat
     3181 tcagattata taaacatttt aaatgggtgt caaaagcaga aatatttaag gttccggtta
     3241 taggctggaa tatggtattg aactgatata tcaaactggt gcgaggcgat aaaaaaagta
     3301 tagcacagat gatggatgag tgtgagatgc atttaaaaga aggtagttcg gtgtatatat
     3361 tccccgaagg ctcacgttcg ccggatggtc atttaaaaca atttaagttg ggagcatttc
     3421 tgttagcaaa aaagttgaat cttcccataa tgcccattat catagaaggt acacgatttg
     3481 cccttcctaa atatagtatc gactatcatg gaaaacatcc tataatatta cgggtacttg
     3541 atgaaataac accggaaaaa tttaaaaaca tgagtgtaga ggagctgata gattatacat
     3601 ggaactatat gaatgctgag ttaaaaaaat tgaggattga gatgtatggc agtgattcaa
     3661 tctgagaata attatacttt aaattagctt gcactattga tgtatatcct gtagagtaag
     3721 aacatatacc attattacag tgaggttgta tcaatgtctc aacctgatga ttttacctta
     3781 gatgacgatt tatttaaatt ggatgatgag atagatatac aggacaatat acccgagaat
     3841 aaacctgaag caaacaacaa tacggtgttg gattcgggaa aagattatgg cgatagttcc
     3901 cgtaaacggt catccagaga tttccagcct gacatggatg cactgctcat cacagcacag
     3961 tcttcaatga ttattgaagg aatgaaaaaa tatactgaac gagatttttc ttcaaataat
     4021 ctccccattt atttagaagc attaaacggc gttaatcttt atataaaaat actagaccgt
     4081 aatccaaaaa attatcataa attaaaatca gtgataaatg ctgatcagga ttgtcaggag
     4141 gttgaaaaga ttgcatttga tttatacaaa aaaatgtt
//
LOCUS       DAOOJH010000156         4161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16690_length_4161_cov_7.599123, whole genome shotgun sequence.
ACCESSION   DAOOJH010000156 DAOOJH010000000
VERSION     DAOOJH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16690_length_4161_cov_7.599123"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..>2356
                     /locus_tag="PLJ75_12175"
     CDS             59..>2356
                     /locus_tag="PLJ75_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HPD06012.1"
                     /translation="MRFAYHFKKWHLFYLFLILIAAYYIVATHPLGTYNAYPDKLSPK
                     EKESSLSTLPLDTRSPCPKFRANGLQNGRTTVQSKASMVQPWEFKTGKGIFSSPVVDE
                     DGTVYIGSADHYFYAITQNGSLKWKCKTNEIIDSSALLDNRGFVYVGSGDSYVYCINR
                     ETGRIVWKSKAHAVQEVQQQFKIKTYNVNWFEGNIGMLPDGSIIAPNDNYLVYELDRA
                     TGQRKTQYIANEMIWSLPAVNIKTERLFFGTQFMALKNIYCYNYRTKELEWTNGGWGS
                     NAASPLLTSSAENGLVIVGGYDGYVRAFAQDSGKQLWKTGLRDHIYASPAQLSNGLIL
                     QPSADGTIYALNPDNGSIVWAYDTLEPIRSSPAIDGNDFIYVGSGDGRLYCLHPDGTL
                     RWSYLCIDEDRNDLNSSPALGKEGIYIGGENGSVFFVPYDYPVHNKSRDKRCMVMQGE
                     MLPDEGAYLVYTNNFGKLFKQPPAMIDANQPLAFTLFVRRNGNTIKSAIDRDTLDINV
                     MGNPLFKVFVSANKQFFVLVPQEVWSSKKSSTITVHIKGNYLSNMRRFGLKFFGGSRG
                     GNFDVTYSFAIAPHTGTMPYAIPLKAGNKQTVFELKRLSAPNPTMLPSWNQIGFDSLH
                     YIGGIVCNSNNAMIVWVIGGKLKDGKTVVDPALEARYPLYLLYDNGLLTLYNYDGFKI
                     NFIGSWDMPFGQYRLSTKVDPITGAIAKAPELFAIALGDEIQFYGRFLKLMGMTEFDT
                     GHMAVFGGLNMNLHTSGIACMPHGVG"
     assembly_gap    2357..2456
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2505..3155)
                     /locus_tag="PLJ75_12180"
     CDS             complement(2505..3155)
                     /locus_tag="PLJ75_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPD06013.1"
                     /translation="MAILPELVKVFIVEDEDLVRDTLSQSLQLIDNFQIVGASANISS
                     SLSIIQSAMPDVCIVDVRLQNENGIEASKILKKLLPNLKIIVLSGYFHETLAATALAS
                     GASAFLSKSIAVSHLVAAIFHVMVHNNFYAPQLPDDVLEGIIINSKTSKRLCALSKRE
                     KEILELIAREYSTREIAEKLGISEKTVRNHKSNIMAKLGITNHVGLIKYAYYMAMI"
     gene            3830..4039
                     /locus_tag="PLJ75_12185"
     CDS             3830..4039
                     /locus_tag="PLJ75_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06014.1"
                     /translation="MVLHALENNLPGIKFDQHSDGIHVIITVPSIPPGSKKFTQINTT
                     IKKNYPLFNVIKFKYLQAMAKTTSH"
BASE COUNT         1274 a          774 c          797 g         1216 t
ORIGIN      
        1 agtaaacaat atactactgt ggttttgtta tccatactgt tgctggggag gtatttctat
       61 gaggtttgca taccatttca aaaaatggca tcttttttat ctttttctga tattgatagc
      121 ggcatactac atagtggcaa cgcatccatt gggaacatat aatgcctatc ccgataaact
      181 atcgcccaaa gaaaaggaaa gctcactctc gacattgccg cttgatacca gaagcccctg
      241 tccaaaattc agggctaacg gactgcaaaa cggcagaacc actgtgcaat caaaggcatc
      301 catggtgcag ccatgggaat ttaaaacagg caaaggtatt ttcagctcgc ctgttgttga
      361 tgaagatggc acggtgtata taggttcggc tgaccattat ttttatgcaa ttacgcaaaa
      421 tggttcattg aagtggaaat gtaaaaccaa tgagatcatt gactcatccg ctcttctgga
      481 caacaggggt tttgtgtatg ttggttcggg cgatagttat gtttattgta tcaaccgtga
      541 aacaggcagg attgtctgga aatcaaaggc acatgcggtg caggaagttc agcaacaatt
      601 taagatcaaa acctataatg ttaactggtt tgaaggaaac attggcatgc ttcccgacgg
      661 cagtattatt gctcccaacg acaactacct ggtgtacgaa cttgacagag caacaggaca
      721 aagaaaaaca cagtacatag ccaatgagat gatatggtcg ctccctgctg tgaacataaa
      781 aacagaaagg cttttctttg gcacacagtt tatggcactg aagaatatct attgctataa
      841 ctacaggaca aaagaattgg aatggaccaa cggtggctgg ggttccaatg ctgcttcacc
      901 gcttttaacc agcagtgctg aaaatggtct ggtgatagtt ggtggttatg atggctatgt
      961 gcgagcattt gcgcaggata gtgggaaaca gctatggaaa accggactga gggatcacat
     1021 atatgcaagc cctgctcagc tttcaaatgg tttgatacta cagccatcag cagatggcac
     1081 tatctatgcc ctcaatcctg ataatggttc tattgtgtgg gcttacgata cgcttgaacc
     1141 aatccgttca tcgcccgcaa ttgacggcaa tgattttata tatgttggtt ccggtgatgg
     1201 caggttgtat tgtttacatc cagatggcac attgcgctgg tcatatctat gcattgatga
     1261 agacaggaat gacttaaaca gttcccctgc tttaggcaaa gaaggcatct acattggtgg
     1321 tgaaaatggg agtgtcttct ttgttcccta tgattatccg gtgcataata aaagcaggga
     1381 taagcggtgt atggtaatgc aaggggaaat gcttcctgat gaaggtgcat atcttgtgta
     1441 cacaaataat tttggaaaac tttttaaaca gccgccagca atgattgatg ccaatcaacc
     1501 gttagcattt acactgtttg ttcggcgcaa tggcaacaca ataaaatcag ccattgaccg
     1561 tgatacgctg gatataaacg ttatgggaaa tcctttattt aaggtttttg tttctgccaa
     1621 caagcagttt tttgtccttg tgccccagga ggtgtggagc agcaaaaaat ccagcaccat
     1681 aacagtgcac ataaaaggaa actatttaag caatatgcgc aggtttggcc ttaaattctt
     1741 tggtggttcc cgaggtggaa attttgatgt aacctattct tttgccatag ctcctcacac
     1801 tggtacaatg ccgtatgcaa taccacttaa agcaggcaat aaacaaacgg tatttgaatt
     1861 gaaacggctt tcagcaccta acccaaccat gcttccttca tggaatcaga ttggttttga
     1921 ttcattgcac tatataggcg ggattgtttg caatagtaac aatgccatga ttgtctgggt
     1981 aattggtgga aagcttaagg atggtaaaac cgtcgttgat ccagcacttg aagcacgata
     2041 tccgctgtat cttttatacg ataatggtct cttaacactg tacaactatg atggctttaa
     2101 aatcaatttc ataggctcat gggatatgcc atttggacaa tacaggcttt ccacaaaagt
     2161 tgatcccatt accggagcta tcgccaaagc acctgaactt tttgccattg cattgggtga
     2221 tgagattcaa ttttatggca gatttttaaa actcatgggg atgaccgaat ttgacaccgg
     2281 tcacatggca gtatttggcg gattgaacat gaacctgcat actagcggta tagcctgtat
     2341 gccgcacggt gttggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnattg
     2461 ttgataccta tccagttgca atggctgagc ttgtcattca atgatcatat cattgccata
     2521 taataggcat atttaatcaa ccctacatgg ttggtaatgc caagctttgc catgatattg
     2581 gatttgtgat tgcgaacagt tttttcactg attccaagct tctcggctat ttccctggtt
     2641 gaatattccc tggcaataag ctccagtatt tccttttccc gttttgacaa tgcgcaaagg
     2701 cgttttgagg ttttactatt tatgattata ccttccagaa catcatcagg taattgtggt
     2761 gcataaaaat tgttgtgaac cataacgtgg aaaatagcag caaccagatg tgataccgct
     2821 atagatttgg ataaaaatgc tgatgctccc gatgctaatg cagttgccgc taatgtctca
     2881 tgaaagtatc ctgataaaac aattattttt agatttggta aaagtttttt taggatttta
     2941 cttgcttcaa tgccattttc attttgaaga cgcacatcaa caatacatac atctggcatt
     3001 gcagattgta taatactgag tgatgaagaa atatttgctg aagcaccaac aatttgaaaa
     3061 ttatcaatta attgcaagct ttgagacagg gtatctctca caaggtcctc atcctcaaca
     3121 ataaaaacct tcacaagttc cggtagaatt gccatatact attcctattt tttttgtgac
     3181 tttaattgta caatatatac aaaaatagtc aataggcaaa tatcccaata tataatttat
     3241 tgttgtatat ataccattgt tgaaatagat attataccat ttgcacaata atcacattat
     3301 agccacttaa atacattatt tttttattat tacagcaatt atctttgaaa agtagcagga
     3361 aaaaaaatag tattgctata ctcactacta caatagagta caacggcact gttatggcgt
     3421 gcagtataga ggttgaacaa tgaacataaa agatattttt taaaaaaaac ctatgataat
     3481 aatccattaa agaaataaaa tgacgaaaca caactatcaa aaaatcagca atctgaaagg
     3541 tatccgcaaa aaataaaata taaaaggtaa tttggactgg gctattgcaa aaggtgctgc
     3601 aattttatat tgcagcacct taatttaata tcaatctctc ttgctggtgt ctttatattc
     3661 ttgattatga agatacaccc tacattggaa gacaaacaaa aggttactca acgagcatta
     3721 ggtcaaaatc gtcgcttcca ttataatatt aacatccatt gcaatccccg tttggattta
     3781 cacctatcaa acatttaaac ttgagcaacc aaaaaaggat gttcaaaata tggtattgca
     3841 cgcgttggaa aacaacctgc caggtataaa atttgatcaa cacagtgatg gtatacatgt
     3901 tattataacg gtaccaagca ttccaccggg ttcaaaaaag tttacacaaa taaatactac
     3961 cattaagaag aactatccat tatttaatgt aataaaattt aaatatttgc aagcaatggc
     4021 taaaacaaca tcacattaaa agaaagaggg ggcaatgcgc tcccctcttt tttgcctcta
     4081 tatagtgata ttctttatcg ctccactgtt actgcacaac cctgcccgcc acctatacat
     4141 aatgttgcta tgccttttct g
//
LOCUS       DAOOJH010000157         4119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16881_length_4119_cov_10.066191, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000157 DAOOJH010000000
VERSION     DAOOJH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_4119_cov_10.066191"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1219
                     /locus_tag="PLJ75_12190"
     CDS             242..1219
                     /locus_tag="PLJ75_12190"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD06015.1"
                     /translation="MIQQPLSPHEQKLISIVPDGYAPMRLDVYLAHRFTYLSRTKWQH
                     EIKTGKIVVNEKQVTNPHYIVYQHYIIEYKRPSVQEPAIIPHYTILYEDDYIIAVNKP
                     GNLPTHPAGKYFHNTLTMLLWNDIGIQCMPLNRLDRETSGVAVLAKSGSIASAFLKYS
                     HTIAKQYYAIVTGNCSFSEITVAMPIGLSPTSSIRKKRSAYTDAHWQAQTHIKVLQHY
                     DTCTLIEATPKTGRTHQIRVHCEHIGHPIIGDTLYCNSDTTFIEYIKNKGALLREFQR
                     CALHAHIYKFYHPFLQKMIAIKAPMPHDMHTFLLRLDKNYGTTNNTTKS"
     gene            1188..1709
                     /locus_tag="PLJ75_12195"
     CDS             1188..1709
                     /locus_tag="PLJ75_12195"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPD06016.1"
                     /translation="MAQPTIPPKAKLFIGLLTSHDEVLFHVINILKKKFGVIDLQSEP
                     IPFSHTDYYSTIGKNLYKVLIGFNRLIKREEIAKIKLFTNKVENKFLKNGNRTINIDP
                     GYLTLSNVFLVSCKDYFHRIYIGRGIYAENEYRYVAKHYHPWEWTYPDYRKKEYLQFF
                     ETLRSIYYEQIKK"
     gene            complement(1722..2741)
                     /locus_tag="PLJ75_12200"
     CDS             complement(1722..2741)
                     /locus_tag="PLJ75_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD06017.1"
                     /translation="MKKLIIIVFLLLLIQSQEFASSRRDTALLYHHNEGEHCIDYIAM
                     YDLLATINTLYLNGYYGLMEKHINNFMKDFPPVKEVFYFKAITLINKNKYDEGLKELF
                     IALAMEPKFSRAQNAAGYIHALRSQWATALQFFREANSNNTYNAFIEYNIAGVYYQLL
                     DFSNALNWSKKVVEHKPNFVQGYYLCALSAMQLNDFASAIEYFNIVIQLKMKNDSMYY
                     NRALAHYFLGNYEKALNDCTTALTINKKNYFAAMLTGIINVNQKNYEKALKVFTDVLK
                     IKKDDLFAKVFQALCIHTINKNISNQLFFAIFGSIDNGEQVAAQLTQLYQLKPVEPLP
                     QFFLY"
     gene            complement(2807..3964)
                     /locus_tag="PLJ75_12205"
     CDS             complement(2807..3964)
                     /locus_tag="PLJ75_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HPD06018.1"
                     /translation="MRFLKFLVAIVMVAGVAAPVFAADSIAKLTLGDDQFIELHYLLQ
                     VQAYSLQKEGATATGDEDYWSKDTKLRRSRIIVKGQAAQNVEFFMETDAPNQEGNESK
                     NLYTQDAYIDFTIAKELKIAFGHILLPFMHHDRQSAATLLCADYTAAVVPVGSNVWRD
                     TGVELRGLLFNGLVDYRVGAWDGQDRKLDTTTNTVVNEDDMPRVTGRIQINLKDAEDG
                     FFYSGNYLGKKSIISFGAGLDYQPDAYDDGSDAKNYYAWTVDAYFDHPIGTNNALTLQ
                     AAYVYVKNSPFFDIDPVAAGTQGADKAKMFFAEGGFLINNTWQPVARFYYKDTESLAG
                     DKNKVSEIAGGLNYYLKGHSANVKVEYAQELKDEADDYKNKRITVQCQIFI"
BASE COUNT         1347 a          869 c          642 g         1261 t
ORIGIN      
        1 ataataccga taactatcga tgtggaggct atacattcag gtttttctta gaagacggga
       61 tacaatgttg ccaattttac tggggaccac cgccggaaat actaaaacga gcactccaaa
      121 catcatgaaa catcaaaatc ttctctaata ctatcgcaca actttattaa atactttatg
      181 aacattttaa agtacagtaa aatagtttac aaaacatgag aatattttca ctataataat
      241 tatgattcaa caaccattat caccccatga acaaaagctc atatccatag tacccgatgg
      301 ttatgctcct atgcgtcttg atgtatattt agcacatcgg ttcacctatc ttagccgtac
      361 aaaatggcaa catgagatca aaaccgggaa gatagtggtt aatgaaaaac aggtaaccaa
      421 cccgcactat attgtttatc aacattacat aattgagtat aagcgcccgt cagttcagga
      481 acctgcaata ataccccatt acacaatact ctatgaagat gattacatca ttgctgttaa
      541 taaaccgggc aatcttccca cacatccagc agggaaatac tttcacaata ccctcacaat
      601 gttgttgtgg aacgacatcg gtatccaatg catgcccctc aaccgtttag accgggaaac
      661 ctctggagtt gcagtgcttg ctaaaagtgg aagtattgca tcagcatttt taaaatattc
      721 acatactatc gccaaacaat attatgcgat agttaccggc aattgttctt tttctgaaat
      781 aacagttgcc atgcccatag gattgtcccc cacttcatca atcaggaaaa aacgctctgc
      841 ctatacagac gcacactggc aggcacaaac acacatcaaa gtgcttcaac actatgacac
      901 atgcacgtta attgaagcca cacccaaaac agggcggact catcagatac gcgtccattg
      961 cgagcatatt ggccacccca ttattggtga taccctttac tgtaacagcg atactacatt
     1021 tattgaatat atcaaaaaca aaggtgctct attgcgtgaa tttcaacgct gcgctttgca
     1081 cgctcacata tacaagtttt atcatccatt cctacaaaag atgattgcaa taaaagcacc
     1141 aatgccacac gatatgcata catttttatt acgattagat aaaaactatg gcacaaccaa
     1201 caataccacc aaaagctaaa ctttttattg gactgttaac ctcccatgac gaggtgttat
     1261 ttcatgtcat caatatatta aaaaaaaaat ttggggttat tgatcttcaa tctgaaccca
     1321 ttccgttttc tcatactgat tactatagta ctatcgggaa aaatctttac aaggtactta
     1381 ttggttttaa caggcttata aaaagagaag aaattgctaa aattaaacta tttaccaaca
     1441 aagttgaaaa taaattctta aaaaacggca accgaaccat caacatagac cccggttacc
     1501 tcaccctgtc caatgttttt ttggtttcat gcaaagatta ttttcataga atatatattg
     1561 gcaggggaat atatgcagaa aatgaatatc ggtatgttgc taaacactat catccatggg
     1621 aatggacata tcccgattat cgaaaaaaag agtacctcca gttttttgaa acactcagaa
     1681 gcatctatta cgagcagata aaaaaataat agtaactgcc atcagtacaa aaaaaattgc
     1741 ggcaatggtt ccaccggttt taattggtaa agctgtgtaa gctgtgctgc aacctgttca
     1801 ccattgtcga tggaaccaaa tatagcaaaa aaaagctgat tggaaatatt tttattaatt
     1861 gtgtgtatac aaagcgcctg aaacaccttt gcaaacagat catccttttt aatttttaat
     1921 acatcagtaa atactttgag agctttttca taatttttct ggttaacatt aataatacct
     1981 gtaagcattg ctgcaaaata atttttttta tttatcgtta gtgcagtagt acagtcattc
     2041 agcgcctttt cataatttcc caaaaaataa tgcgctaatg cacgattata atacatagaa
     2101 tcatttttca tcttcagttg tatcacaata ttaaaatatt caattgctga tgcaaaatca
     2161 tttaattgca tagcactgag ggcacataga taatacccct gaacaaaatt tggtttgtgc
     2221 tctacaacct ttttgctcca gtttagcgcg tttgaaaaat caagtaactg ataatagaca
     2281 cctgctatat tatattctat aaatgcgtta taggtattgt tactgtttgc ctcacgaaag
     2341 aactgcaatg ctgtagccca ttgactgcgc aatgcatgga tatatcctgc agcattttgc
     2401 gcacgcgaaa attttggttc cattgccagt gcaataaaaa gttcttttaa cccctcatca
     2461 tatttatttt tattaatcaa tgttattgct ttaaaataaa aaacttcttt tactggtgga
     2521 aagtccttca taaaattatt tatatgcttt tccattaaac catagtagcc atttaaatat
     2581 aatgtattga tagtagccag aagatcatac atggcaatat aatcgatgca atgttcaccc
     2641 tcattatggt gatataataa cgctgtatca cgtcgcgaag atgcaaactc ctgtgattga
     2701 ataagaagta acaaaaaaac aataataatt aattttttca tgtatacctt tttatgctaa
     2761 aaaggctgcg cgctgcacag ccttttttat caatgcattg catacatcat ataaaaatct
     2821 ggcactgaac agttatcctc ttgtttttat aatcatctgc ctcatccttc aattcctgag
     2881 catattctac cttaacgttg gcgctatgcc cttttaagta atagttcaat ccaccagcaa
     2941 tctcggacac cttgtttttg tcacctgcta atgattcggt atctttataa taaaaacgag
     3001 caactggctg ccatgtgtta ttgatcaaga aaccaccttc tgcaaaaaac atctttgctt
     3061 tgtcagcacc ttgagtaccg gcagcaacag ggtctatatc aaaaaaagga ctatttttta
     3121 catatacata cgctgcctgc agtgttagcg cattgtttgt accaatagga tgatcaaaat
     3181 aggcatcgac tgtccatgca taataatttt ttgcatcact gccatcatca taagcatcgg
     3241 gctgataatc caatccagcg ccaaagctta tgatgctttt tttacccaga tagttgccgc
     3301 tataaaagaa accatcctca gcatctttaa gattaatctg aatacgtccg gttactcgtg
     3361 gcatgtcatc ttcattaaca actgtgtttg tcgttgtatc taattttcta tcctgtccat
     3421 cccatgctcc aactcggtaa tcaaccaatc cattgaaaag cagccctctc aattcaacac
     3481 cagtatcacg ccacacattg ctaccgacag ggacaacggc tgcggtataa tcggcacaca
     3541 aaagcgttgc ggctgactga cggtcatgat gcatgaatgg taaaaggata tgaccaaacg
     3601 ctatcttcag ttcctttgca atagtaaaat caatatacgc atcctgagtg tataaatttt
     3661 ttgactcatt accctcctgg tttggcgcat cggtttccat aaaaaactca acattctgag
     3721 cagcctgtcc tttcacaatg atacgggagc gtcgcaactt tgtatctttt gaccagtaat
     3781 cctcatcgcc tgtggcagtg gcgccttctt tttgcaatga atatgcctgc acctgtaaca
     3841 gatagtggag ctctataaac tgatcatcac ccaacgttaa cttagcaata ctatccgcag
     3901 caaacaccgg tgcagctacg ccagcaacca taacaatcgc aactaaaaat tttaaaaaac
     3961 gcatacactg atcctcctta aaaatttttt agattttttg caaaaacatt ggtaaagata
     4021 ataatttttg cttttgtatt tcacagggtt attttttcaa tgattttttg tattaattgc
     4081 cctgtgaata tctttttaat tcaattgaat aacctcaac
//
LOCUS       DAOOJH010000158         4100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_16971_length_4100_cov_9.719901, whole genome shotgun sequence.
ACCESSION   DAOOJH010000158 DAOOJH010000000
VERSION     DAOOJH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16971_length_4100_cov_9.719901"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..483
                     /gene="bfr"
                     /locus_tag="PLJ75_12210"
     CDS             13..483
                     /gene="bfr"
                     /locus_tag="PLJ75_12210"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HPD06019.1"
                     /translation="MKGDPKLIETLNALLADELTAINQYMVHSEMCDNWGYEKLHKHF
                     EKRAIDEMKHAEKLIGRILFLEGIPIVTNLKKILIGADVPKQLANDHAAEEDAIKAYN
                     NAIALAGDVKDFATREVLEKILNDEDMHIDAIEELQDQINQMSLQIFLTTQVKE"
     gene            complement(504..1094)
                     /locus_tag="PLJ75_12215"
     CDS             complement(504..1094)
                     /locus_tag="PLJ75_12215"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPD06020.1"
                     /translation="MAQITWKPGTMLYPVPAVLITSSFQGRDNVCTVSWVGTVCTEPP
                     MIGISLRPERYTYELIKKSKEFVINIPDKKLVKAVDFCGVRSGKKYDKFSIAHLTRLP
                     AEKVKAPLIEECPISIECKVIKILNLGSHNQFIGKVLLVHVDDALLDKRGALHLERAG
                     LLCYNHGFYCAVDKQIGKFGFSVKKRKKEKTKSTLR"
     gene            complement(1161..2540)
                     /gene="mgtE"
                     /locus_tag="PLJ75_12220"
     CDS             complement(1161..2540)
                     /gene="mgtE"
                     /locus_tag="PLJ75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPD06021.1"
                     /translation="MIAPLIQPEIKELIDNREFSVLKEVFSEWAPADLAELISELPED
                     EQIIIFRLLPKQLQSQTFEYLEYDIQEHLLSNLAKKQIADILNEMSPDDRTALFEELP
                     ADLLRQMLALLSKEERVTAMTLLGYPENSVGRLMTTDYIAVKENMTIAQVLHYIRKYG
                     KDSETLNMLYVVDDNGRLIDDIKMRQVLLSQPTRRIKTLMDHKFVSLNAFDDQETAIQ
                     VFKKYDHYALPVIDADGALLGIVTIDDVLDVEEEEVTEDIHKMAAVEALKEPYIDEPI
                     LRLTRKRATWLVVLFIGEMLTATAMGYFEHEIAKAVVLALFVPLIISSGGNSGSQAST
                     LVIRAMAVGEITLKDWWKVLLREIQSGFLLGVILGSIGFLRISIWQRFSDMYGQHWIG
                     IAISVALALLGVVMWGTTVGSMLPFILRRLGLDPATSSAPFVATLVDVSGLIIYFNVA
                     YLLLKGSLL"
     gene            complement(2729..3607)
                     /locus_tag="PLJ75_12225"
     CDS             complement(2729..3607)
                     /locus_tag="PLJ75_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HPD06022.1"
                     /translation="MQILIVAVAILIIILIYKGVRIVPQAENWLVERFGKFAAVLTPG
                     LNLINPLFSKVSAKIDIREQVLDLPPQGIITEDNAAVKVDGVVFFKILDPYKAFYGIE
                     NLNMAISNLALTTLRSIMGKMTLDQSLSSRDKINAELLIILDQATDSWGTKITRVEIK
                     DIVPPQDLQQAMALQMKAERERRATVLEAEAQKEAQEKKAEGFKRAQILEAEARKESA
                     QRDAEARERLAQAEADAIEFVAKSLKSTGGDPLLYLLGMEYVKGLIKLGESQSSHVVI
                     LPSDLIENLKGLFKLK"
     gene            complement(3651..>4100)
                     /locus_tag="PLJ75_12230"
     CDS             complement(3651..>4100)
                     /locus_tag="PLJ75_12230"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPD06023.1"
                     /translation="MELSPSAWLTIGIILMAVEILLPGFIIFWFGIGALITAVLVYSG
                     LVESYTWQWFCFFISSGAFLGLWFGYLKDKVKVKTADATPDVTLTGKKGKCTQDIIPP
                     QIGEVELYEPFHGVKIWKAQSNEHIVKGEQIVVAGADGIKLIVKKHQ"
BASE COUNT         1328 a          852 c          635 g         1285 t
ORIGIN      
        1 aggagaataa ttatgaaagg cgatccaaaa ttgattgaga cattgaatgc attgcttgct
       61 gatgaattaa cagcaatcaa tcaatatatg gttcactcag aaatgtgcga taactgggga
      121 tatgaaaagc tccacaaaca ctttgaaaaa agagccattg atgagatgaa acatgctgaa
      181 aaactcatcg gcaggatatt gttccttgaa ggaataccaa ttgttacaaa tttaaaaaaa
      241 atcttaattg gagctgatgt cccaaaacaa ctggcaaatg atcatgctgc cgaagaagat
      301 gctattaaag cgtataataa tgcaattgca ctggcaggtg atgtaaagga ttttgcaaca
      361 cgtgaggtac tggaaaaaat attgaacgat gaagatatgc atattgatgc aatcgaagaa
      421 ctgcaggatc agataaatca gatgtcatta cagatatttt taacaacaca ggtcaaagaa
      481 taatctttta tattccacgc tgcttaccgt agcgtggatt ttgttttttc tttttttctt
      541 tttttaactg aaaacccaaa ttttcctatt tgtttatcaa ctgcacaata aaaaccatga
      601 ttataacaca gtagtcctgc cctttccagg tgcaaagcac ctctcttatc taataatgca
      661 tcatccacat ggaccagcaa aactttccca ataaactgat tatgactgcc taaatttaaa
      721 atttttatta ccttacattc tattgaaata gggcattctt caatgagtgg cgcttttact
      781 ttttctgctg ggagcctggt aagatgagct atcgaaaatt tatcatattt ttttccagaa
      841 cgcacaccgc aaaaatctac tgctttcacc agttttttat ccggaatgtt aataacaaat
      901 tcttttgatt ttttaataag ttcatatgta taccgttccg gacgcaacga tatccctatc
      961 attggaggct ctgtacatac cgtaccaacc cacgatactg tgcatacatt atctctgccc
     1021 tgaaaactgg atgttatcaa cacagcaggc acaggatata acattgtacc tggtttccac
     1081 gttatttgtg ccataatgca aactccataa tagtatagtc accctcaaaa atcaaattat
     1141 cagaggttta ctaatagctt ttataacagt gatcctttta ataaaagata tgcaacattg
     1201 aaatagatta ttaatcccga aacgtcaacc agtgttgcaa caaacggggc agaagatgtt
     1261 gcagggtcaa gcccaagtct gcgtaaaata aatggcaaca ttgaaccgac ggtagttccc
     1321 cacatgacta ctcccaataa tgccaatgct accgaaatcg caatacctat ccaatgctga
     1381 ccatacatgt cagaaaatct ttgccatata ctaatcctca agaaccctat tgaacctaaa
     1441 attacgccta ataaaaatcc tgactgtatc tctcgcaata ataccttcca ccaatctttt
     1501 aacgtaatct cacccactgc cattgcgcgt ataaccagtg tagatgcctg agaaccggaa
     1561 ttgccaccac ttgatataat caatggcaca aaaagagcaa gtacaacagc tttagcaatt
     1621 tcatgctcaa aatatcccat tgcagtagct gttaacatct caccaataaa tagtaccacc
     1681 aaccatgttg ctcttttcct ggttaatcgt aatataggtt catcaatata tggctcttta
     1741 agagcttcaa ctgcagccat cttatgaata tcttctgtga cctcttcttc ttcaacatcc
     1801 aatacgtcat caatagtaac tatccccagt aatgctccgt ctgcatcaat taccggcaga
     1861 gcataatggt catatttttt aaaaacctgt attgccgttt cctggtcatc aaaggcattg
     1921 aggcttacaa atttatgatc catcaaagtt tttatgcggc gtgtaggttg tgatagcagt
     1981 acctgtctca tttttatatc atcaattaac ctgccattat catcaactac atataacatg
     2041 ttgagtgttt cactatcttt cccatatttt cgtatataat gcaaaacctg tgcaatggtc
     2101 atattttctt tgacagctat ataatcagtg gtcattaaac gaccaacgct attttcggga
     2161 tacccaagca aggtcattgc agtaacacgc tcttcttttg aaagaagagc taacatctgg
     2221 cgtaaaagat cggcaggcaa ctcttcaaat aatgcagttc ggtcatcagg tgacatctca
     2281 ttcaagatat cagctatctg ctttttggct aaattgctta agaggtgttc ctgtatgtcg
     2341 tattccaggt attcaaatgt ctgagattga agttgttttg gtaaaagacg aaaaattatg
     2401 atctgttcat cttcgggcag ttcgcttatc agctcagcta aatcagcagg cgcccattca
     2461 ctgaatactt ctttaagaac actaaattct ctattatcaa taagttcttt tatttcgggc
     2521 tgaataagag gagcaatcat cataatgtgc ctcagaaaaa taaaaaataa ttaacaacta
     2581 aatgtgaaaa tcctgcatca aaaaaatcaa tacagtttta ataaataata aagagtttta
     2641 agcttataat gatatatata taaaacgtaa atccttgaat atttaagtgc taatcatttt
     2701 actcatcaac aatcggatga caaatctttc attttagttt aaataaacct ttcaaatttt
     2761 caataagatc cgatggtaaa ataaccacat gactgctctg tgattcaccc aattttatca
     2821 atccttttac atattccata ccaagaagat ataataatgg gtctccaccg gtacttttca
     2881 atgattttgc aacaaattct attgcatcag cttctgcttg agctaaacgt tcacgagctt
     2941 ctgcatcacg ttgagctgat tccttccgtg cttcagcttc aagtatctga gcacgtttaa
     3001 atccttctgc ctttttttcc tgtgcttctt tctgggcttc tgcctcaagt actgtcgcac
     3061 ggcgttcacg ctcggctttc atctgcaaag ccattgcctg ctgcaaatcc tgtggaggaa
     3121 ctatatcctt gatttcaacc cgtgttattt tagtgcccca tgaatctgtt gcctgatcca
     3181 atataatcag caactctgca ttaattttat cgcgggaaga taatgattga tccagagtca
     3241 ttttacccat gatggaacgt aacgtggtta acgccagatt agagattgcc atatttaaat
     3301 tttcaatacc ataaaacgct ttataaggat caagaatttt gaaaaaaaca acaccatcaa
     3361 cttttactgc tgcattatct tcggttatta taccctgtgg cggcagatcc agtacctgtt
     3421 cacgaatatc tatctttgca gatacttttg aaaataatgg attaataagg tttaatcctg
     3481 gagtcaatac agctgcaaat tttccaaatc gttccacaag ccaattttct gcctgaggta
     3541 caatcctgac tcctttatag ataaggataa tgatgagtat agctacagca acaattaata
     3601 tttgcatatc aaacctctct atattatata gtatataaaa aatgttttca ttattggtgt
     3661 tttttaacta tcaatttaat gccatcagca cctgcaacaa ctatttgttc ccctttaaca
     3721 atatgttcat ttgattgagc tttccagatc tttactccat gaaatggctc atacaactcc
     3781 acctcaccaa tctgtggagg tataatatcc tgagtacatt ttcctttttt acctgttaat
     3841 gttacatcag gagtagcatc agcagtctta acttttactt tatcttttag atagccaaac
     3901 cataacccta aaaaagcccc tgaagatata aaaaaacaaa accattgcca tgtatatgat
     3961 tctaccaaac ctgaataaac aagtactgcg gtaatcaatg ctccaatacc aaaccaaaaa
     4021 ataataaatc ccggcaatag tatctctact gccataagaa taataccaat tgttaaccat
     4081 gcagatggcg atagttccat
//
LOCUS       DAOOJH010000159         4092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17014_length_4092_cov_7.771117, whole genome shotgun sequence.
ACCESSION   DAOOJH010000159 DAOOJH010000000
VERSION     DAOOJH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17014_length_4092_cov_7.771117"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /gene="rfbB"
                     /locus_tag="PLJ75_12235"
     CDS             complement(<1..929)
                     /gene="rfbB"
                     /locus_tag="PLJ75_12235"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002792369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPD06024.1"
                     /translation="MDSILITGGCGFIGSNFIEYCIDKYDYHIINIDALTYAANPDNV
                     KEVANHKRYTFIHGNICDRELLQSIFHKYTIKGVIHFAAESHVDNSIKDAENFITTNI
                     VGTHTLLQCAYRSWMEKPFVYKHDYRNCRFHHISTDEVYGSLGSSGYFTELSPYAPNS
                     PYSASKASGDMLVRAYHHTYGLNTVITNCSNNYGPKQHDEKLIPTIIRNALHGNPIPI
                     YGDGKNVRDWLYVLDHCKGIDVVFHKGKAGQTYLIGGHNEHDNNAIAKKICSLLDSMY
                     PVHNNSIINEGKRTISSYADLIHYVEDRPGHDKR"
     gene            complement(930..1805)
                     /gene="rfbA"
                     /locus_tag="PLJ75_12240"
     CDS             complement(930..1805)
                     /gene="rfbA"
                     /locus_tag="PLJ75_12240"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010137043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPD06025.1"
                     /translation="MKGIILAGGSGTRLYPITKATCKQLLPVYDKPLIYYPLSVLMLS
                     GINDILIISTPQDSERFYNLLGDGSWLGIKLSYAVQEKPNGIAEAFLIGKNFIGNDTV
                     ALVLGDNILFGHGLIAHLLDSKTIVHTDNHAVIFAYYVKDPQQYGVVEFDEKLNAISI
                     EEKPAKPRSNYAVIGLYFYPNDVVKKAKSITPGNRGELEITSINQLYLDQNNLKVKVM
                     GRGFAWLDTGTHDSLLEASNFVKIIEERTGLKIGCVEEIAFKNGWITKEQVLLIAKSL
                     SKTDYSQYLITIAGK"
     gene            complement(2114..3325)
                     /locus_tag="PLJ75_12245"
     CDS             complement(2114..3325)
                     /locus_tag="PLJ75_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPD06026.1"
                     /translation="MKTFKVLSIAAMVLFVAANAFATNGMRMIGFGPVQRSMGGIGVA
                     TTLDAASIITNPAGIADLKGRIDFGASYFSPTVKHKASGPGTMAKDGETVESDAGASP
                     VPAFGLVIPLTEKLHFGIGAYGVSGMGVDYEKNVYQSILKTMYSQMRFAPGLSYKIND
                     MISVGAVANIMYATMEFSAATNASVIGMSDQEEHMNSAAFGYGATIAVNIKPVDMLTI
                     GVAYETKSNFKDFEFNTDQGKDKLEFDQPQSVTAGVSVTPLKDLTVAFEVQWIEWSET
                     NGKNKPEFSKNSSNATPWNMNWDNQMVYKIGAQYAINPMFTVRAGFNYGKMPLDEDRA
                     FENVAFPAIAEYHYTAGIGITFDNNLTLNIGGMYSPEAKIDGANMSQNFAHYETKMSQ
                     YSLDMGIAYVF"
     gene            complement(3851..>4092)
                     /locus_tag="PLJ75_12250"
     CDS             complement(3851..>4092)
                     /locus_tag="PLJ75_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPD06027.1"
                     /translation="HTILLGQCMVNLHKHNTAINAIPVKGCPPNPMETVTALHKAGIE
                     VNPLIFEHLDSAPSYFMERYKNKPEFDEGFFTIKE"
BASE COUNT         1173 a          813 c          786 g         1320 t
ORIGIN      
        1 cgcttatcat gccccggacg gtcttcaaca tagtgtatta aatcggcata cgaggatatg
       61 gtacgcttac cctcattgat tattgaattg ttatgcactg gatacatgct gtcaagcaat
      121 gaacatattt tttttgcaat ggcattgttg tcatgctcat tatgcccgcc aatgaggtat
      181 gtttgtcccg ctttgccttt atgaaaaaca acatcaatcc ctttgcaatg atcaagcaca
      241 tataaccaat ccctgacatt tttgccatcg ccatatatgg gtatgggatt tccatgcaac
      301 gcatttcgta tgatggtggg aataagcttt tcatcgtgct gctttggacc atagttgttt
      361 gagcaattgg taataacggt atttaatccg taggtgtggt gataggctcg caccagcata
      421 tcaccggatg ccttgctggc gctataagga ctgttggggg catagggcga tagttctgta
      481 aaatagccgc tactgccaag cgagccatac acttcatcgg ttgaaatatg atggaagcga
      541 cagttccggt aatcatgttt atagacaaaa ggtttttcca tccacgagcg ataggcacac
      601 tgtaaaagag tgtgcgtccc aacgatattg gtggtaataa agttctcggc atctttgatg
      661 gagttgtcca catgactttc agcagcaaag tggatgacac ctttgatagt atatttatga
      721 aagatggact gcagcaactc tctgtcacag atgttaccat ggataaaggt atagcgttta
      781 tggttagcta cctcttttac attgtcgggg tttgcagcgt aggtcagcgc atcaatattg
      841 ataatgtgat agtcatactt atcaatacaa tattcaataa aattgctccc aataaaacca
      901 cagccaccgg taattaaaat agaatccatt tattttcccg caatagtaat tagatattgg
      961 ctataatctg ttttggatag tgactttgca atcaaaagaa cctgctcttt tgtaatccag
     1021 ccgtttttaa atgctatctc ctccacacag ccaattttta gcccggtacg ctcctcaata
     1081 atttttacaa agttacttgc ctccagaagg ctatcatggg taccggtatc aagccatgca
     1141 aaacctcttc ccataacctt aaccttcaaa ttattttgat ctaaatataa ttggttaata
     1201 ctggtaattt caagctcgcc acggttgcct ggtgttatgc ttttggcttt ctttactaca
     1261 tcattggggt aaaagtacag tccaataaca gcatagttgc tcctgggttt tgctggtttt
     1321 tcctctattg agatggcatt gagcttttca tcaaattcaa caacaccgta ttgttgaggg
     1381 tctttaacat aatacgcaaa gatgacagca tggttgtcag tatggactat tgttttgctg
     1441 tccagtaaat gtgcaatgag cccatgtcca aagagtatgt tgtcacccaa taccagggca
     1501 acagtatcgt tgcctataaa atttttacca attaaaaaag cctctgcaat accattgggt
     1561 ttttcctgca cagcgtacga tagttttatt cccagccagc tgccatcacc cagaagatta
     1621 taaaaacgtt cactgtcctg aggtgttgaa attatgagta tatcattaat accggaaagc
     1681 atcaacactg aaagaggata atagataagg ggtttatcat aaacaggcaa tagctgcttg
     1741 catgttgctt ttgtaattgg atacaggcgt gttccggaac cgcctgctaa tataatcccc
     1801 ttcatattgg tgaaattact ctttatttta ttaaaaatta acgaatgcta ttactgataa
     1861 atgtttacga aaaaaattgt ttgtgtcaat tattatttta tgctggttag ggaaaattat
     1921 ataactatta agcgttttat aataagtatt gaaagggatt ttaaaaacct aaagataaac
     1981 cccggcccat ctcactggta aggcaagggg aaacctcacc cctgtttcct cgccttcata
     2041 ggacttgtag taataaaaaa atgagaggga tagccctctc atttcatggg attacgtaac
     2101 agtataaatc tttttagaat acgtacgcta tgcccatatc aagtgaatac tggctcattt
     2161 tagtttcata atgggcaaaa ttttgagaca tattagcacc gtcaatttta gcttcaggtg
     2221 aatacatgcc gcctatatta agcgtaaggt tgttatcaaa tgttatgcca ataccagcgg
     2281 tatagtgata ttcggctata gcagggaatg ccacgttttc aaatgctctg tcttcatcta
     2341 acggcatctt gccatagtta aagcctgctc gcacggtaaa cattggattg attgcatact
     2401 gggcacctat tttatacacc atctggttgt cccagttcat gttccatggc gttgcattgc
     2461 tgctgttttt gctgaattca ggcttatttt tgccgtttgt ttcggaccat tctatccact
     2521 gaacctcaaa cgctacagta aggtctttca atggggttac tgaaacacct gctgttacac
     2581 tctgaggctg gtcaaattcc aatttatctt tgccctggtc ggtattaaat tcaaaatcct
     2641 taaaattgct ctttgtttca taggcaacac caatagtaag catatcaacc ggtttgatat
     2701 tgacagcaat ggttgctcca tacccaaatg cggcagaatt catgtgctct tcctggtcag
     2761 acattcctat tacactggca ttggttgccg ctgaaaattc cattgttgca tacataatgt
     2821 ttgcaacagc gccaaccgat atcatatcat ttatcttgta ggataaccct ggagcaaagc
     2881 gcatctgact atacatggtt tttaagatac tctggtaaac atttttttca tagtcaacgc
     2941 ccataccgga aacaccgtag gcgccaatac caaaatgtaa cttttcggtt agcggtataa
     3001 caagcccaaa tgccggtact ggtgaagcgc ctgcatctga ttcaacggtt tccccgtctt
     3061 ttgccattgt ccctggaccg gaagccttat gctttaccgt tggtgaaaaa tatgaagcgc
     3121 caaaatcaat acgccctttt agatcagcaa tacctgctgg atttgtaatg atggaagccg
     3181 cgtcaagcgt agtggcaact ccaataccac ccatagaacg ctggaccgga ccaaagccaa
     3241 tcatgcgcat gccgttggtg gcaaatgcat ttgcagctac aaacaaaacc atggctgcaa
     3301 tggataaaac cttaaaagtt ttcataaaac cctcctcacg aagttatttg aactgtattg
     3361 ttataattat agatttttta gatacaatca atgtagcaag gggttgttta gatgtcaaga
     3421 aaattataat attattaaat aaatattttg tataattaaa ttatactata atacttaatc
     3481 actggataaa ttattgatat aatgcacagc tatgaacctt ttatagatac ttttcatgtc
     3541 tatgttgtat atatagatga ttttattatt attactggat tagtgggata tttgtactat
     3601 ctgcttcacg attgtaagct ctccctatta caattgttcg gaaggcatca cccggttcat
     3661 ttattgtttt tactcattat cgtactatgg aatgtttacg ttcaggatta cacgatgtca
     3721 ttaataactg ttgtagtttt tataggggct ctccatcttt ttatgatggc aatatttatt
     3781 caatcatcat gtgattggta aacgctgtat taaatgatgt ggcgagtgcc agtaaatttt
     3841 ttaagcctca ttattctttt atagtaaaaa atccctcatc aaactctggt ttgtttttat
     3901 accgttccat aaaataggat ggtgcactat ccagatgctc aaagataagc ggattaacct
     3961 caatccctgc tttgtgtaat gccgtaacgg tttccatggg gttgggagga cagcctttaa
     4021 ccggtattgc attgatggca gtgttatgct tatgcagatt taccatacat tgtccaagca
     4081 atatggtgtg ct
//
LOCUS       DAOOJH010000160         4089 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17029_length_4089_cov_9.978929, whole genome shotgun sequence.
ACCESSION   DAOOJH010000160 DAOOJH010000000
VERSION     DAOOJH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_4089_cov_9.978929"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..3347
                     /gene="carB"
                     /locus_tag="PLJ75_12255"
     CDS             144..3347
                     /gene="carB"
                     /locus_tag="PLJ75_12255"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPD06028.1"
                     /translation="MPKRSDIHKIMIIGSGPIIIGQACEFDYSGTQACKALKKLGYTI
                     VLVNSNPATIMTDPEMADITYIEPLTVDSLSKIIEKEKPDALLPNLGGQTGLNLSSSL
                     YKTGVLQKHGVQIIGVDIDAIERGEDRISFKNTMQQLGIAMPASEPAFSVDEAKKIAE
                     RSGFPLVIRPAYTLGGTGGGHVYNMEDLEIIVSRGLSASMIGQVLVEESVTGWEELEL
                     EVVRDAKNQIITVCFIENVDPMGIHTGDSFCTAPMLTISSETQKRLQQYAYRIVEAIK
                     VIGGTNIQFAHDPKTDRIVVIEINPRTSRSSALASKATGFPIAFVSSLLAAGLTLDEI
                     PYWRDGTLDKYTPSGDYIVVKFARWGFEKFKGVEDKLGTQMRSVGEVMSIGKNYKEAF
                     QKAIRSLEIGRHGPGFAKDFNKLPLEELIRLLSTPSSERHFIMYEALRKGADIAILKE
                     RTQIKEYFIQQMKELVDLENEMLPFAKKSLPDSLLKTAKEWGFSDRYLSKILELPEDA
                     IRKQRISLGVVERWEAVPVSGVEDAAYYYSTYNAPDTVSVSHRKKIMVLGGGPNRIGQ
                     GIEFDYCCVHAAFAIKEAGYEAIIINCNPETVSTDYDTADKLYFEPLTVEDVLSIYEK
                     EKPEGVIVQFGGQTPLNIAGELVKAGVKILGTSVDSIDLAEDRDRFRKVITKLSIPQP
                     ESGMASSLEEALSIANQIGYPLMVRPSYVLGGRAMEIIYDEEMLKQYVKAAVEVTPER
                     PILIDRFLVDAIECEADAICDGENVFVPSVMEHIELAGVHSGDSACAIPPDNIKKEHI
                     ETIEKYTKMLALELNVIGLLNIQYAIVNDTVYILEANPRASRTVPLVSKVCGIPMAKI
                     ATKLMLGSKLSPDLTVKKKIKHFGVKEAVFPFNMFPEVDPVLGPEMRSTGEVLGIANS
                     FGVAYFKAQDAAGWRLPLEGNILITVNRKDRESALEVAKKFHSLGFTIIATEGTSKYL
                     DEHSIPTQMVFKLNEGRPDVVDRIKNKEINLIINTPFGRISEVDDSYIRKAAIKYKIP
                     YITTIAAARAAVRGIEMIKRYGEETPKSLQQYHSEMS"
BASE COUNT         1332 a          761 c          893 g         1103 t
ORIGIN      
        1 caagtactaa tcttgggtaa ctgctatgaa tttcaactaa aacagggaca tgctctattc
       61 attaattata aaataacata ttttattatt gacattattc ttccccatgg agctttttat
      121 ttaaaaatga gcggaggaac accgtgccaa aacgaagcga tatacataag ataatgatta
      181 ttggttctgg tcccattatt attgggcagg catgcgagtt tgattattca ggaacgcaag
      241 cctgcaaagc tttaaaaaaa ttagggtaca cgatagttct tgtcaattcc aatccagcaa
      301 caattatgac cgaccccgag atggctgata ttacctatat tgaaccactg actgtagatt
      361 cactttctaa aattattgaa aaagaaaagc ctgatgcatt attgccaaat ttaggaggac
      421 agacgggatt gaatctgtcc tcttctttat ataaaactgg tgttttacaa aaacatggag
      481 tacagattat tggtgtcgat attgatgcaa tagaacgcgg tgaagaccgc atttctttta
      541 aaaacaccat gcaacagctt gggattgcaa tgcccgcaag cgaaccggca ttcagcgttg
      601 atgaggcaaa aaaaatagct gaaagatcag gattccccct ggttatacgc cctgcctata
      661 ctcttggagg aacaggcggc ggtcatgttt ataatatgga agatcttgag attattgttt
      721 cacgaggact atcggcaagc atgattggtc aggtgctggt tgaggaatcg gttacgggct
      781 gggaggagct tgaattagag gttgtacggg atgcaaaaaa tcaaattata acggtttgct
      841 ttatagaaaa cgttgatcca atgggtatcc acaccggtga ttcattttgt acagcgccca
      901 tgcttaccat aagctctgaa acacaaaaac gtttgcaaca gtatgcatac cggattgttg
      961 aagcaatcaa agtcattggg ggtaccaata ttcagtttgc ccatgatccc aaaactgaca
     1021 ggatagttgt cattgagatt aatcccagaa cttcacgctc gtcggcattg gcatcaaagg
     1081 caacgggatt tcccattgca tttgtatcat cactacttgc tgctggatta actctggatg
     1141 aaatccctta ctggcgtgat ggtactcttg ataagtatac accatcaggt gattatatag
     1201 tggtaaaatt tgcccgctgg ggttttgaaa aatttaaggg tgttgaggat aagcttggca
     1261 cacagatgcg ttcggttggc gaggtcatga gcataggaaa aaattataag gaggcttttc
     1321 agaaggcaat acgttcttta gagattggcc ggcatggacc cggctttgct aaagatttta
     1381 ataagcttcc acttgaagaa ctgatacgcc tgctcagtac accatcaagc gaacgacact
     1441 tcatcatgta tgaagccttg cgaaaaggtg ccgatattgc aattttaaaa gaacgcacac
     1501 aaataaaaga atattttata caacaaatga aagagcttgt tgatttagaa aatgagatgc
     1561 ttccatttgc aaaaaaatca cttcccgact ctcttttaaa aactgcaaag gagtggggat
     1621 tttctgatag atacctttca aaaatacttg aacttcctga agatgcaatc cgcaaacaaa
     1681 gaatatcgct gggagttgtt gagcgatggg aagctgtacc ggtaagcggt gtagaagatg
     1741 ctgcttatta ctattccaca tacaatgcac ccgatacagt atcagtatca catcgtaaaa
     1801 agataatggt gcttggagga ggacctaaca gaattggtca gggcattgaa tttgattact
     1861 gttgcgttca tgcagctttt gctataaaag aagctggata tgaagcaatc attatcaact
     1921 gcaatcccga aacggtatcc accgattatg atactgctga caagctgtat tttgaaccac
     1981 taacagtaga agacgtactg agtatttatg aaaaagaaaa acccgaaggt gttattgtac
     2041 aatttggcgg gcagactccg cttaatattg caggtgaact ggtcaaagca ggggtaaaaa
     2101 ttttaggaac ttctgtcgat tctattgatt tagcagaaga ccgtgatcgt tttagaaaag
     2161 taattacaaa attaagcatc ccgcagcctg aatcggggat ggcatcatcg cttgaagaag
     2221 cgttgtcgat tgccaaccaa ataggatacc ctcttatggt acgcccttca tatgtactgg
     2281 gtggtagggc aatggaaata atatatgatg aagagatgct taaacaatac gtaaaagctg
     2341 cggtagaagt aacccctgaa aggccaattt tgatagaccg ttttcttgtt gatgctatag
     2401 agtgtgaagc tgatgccatc tgtgatggtg aaaatgtttt tgtcccttct gtgatggaac
     2461 atattgagct tgcaggtgtt cattccggag attcagcatg tgctatccct cctgataaca
     2521 taaaaaaaga acatattgaa actattgaaa aatataccaa aatgcttgca cttgagctta
     2581 acgttatagg gcttttaaat atacagtatg ctattgtaaa tgatacagta tatattcttg
     2641 aagcaaatcc aagggcatca cgtacggtac cacttgtttc aaaagtatgt ggcataccaa
     2701 tggcaaaaat tgctacaaag ttaatgcttg gcagcaagct atctcccgat ttaacagtta
     2761 aaaagaagat taaacacttt ggtgttaaag aagcagtttt cccatttaat atgttccctg
     2821 aagttgaccc tgttttagga cctgagatgc gttctaccgg ggaggtgctt ggcatagcca
     2881 atagctttgg cgttgcatat tttaaagcac aggatgctgc aggatggcga ctgcccttag
     2941 agggcaatat actcattact gtaaatcgca aagatcgtga aagcgctctt gaagttgcaa
     3001 aaaaatttca ttcactggga tttacaataa ttgcaactga aggtacatca aaatatcttg
     3061 atgaacacag tataccaaca cagatggttt ttaaactcaa cgagggaaga ccggatgttg
     3121 ttgacaggat taaaaacaaa gaaattaacc tcataatcaa cacaccattt ggccggataa
     3181 gtgaagtgga tgattcatat atccgtaaag ctgcaataaa atataaaatt ccatatatca
     3241 ccaccattgc agcagcaaga gcagcagtca gaggtattga aatgataaaa cgctatggtg
     3301 aagaaacacc aaaatcacta cagcaatacc attcagagat gtcgtaattt ggtataaaaa
     3361 tgatgttgag ggtgcgatgt actccccctt aaacgaaagg gggaattatc aaggctctga
     3421 ccccatttta taagtatcga ttctatccct atgggattag actgcaaaag caattatgaa
     3481 gaattaacag catattttat gctgataatg acttattact gtatatgaac gaaaaaacag
     3541 cgaaggcacg gatgccgtag cgccatacgg ctcgtgcgag gacaaagaca ggatgtcttc
     3601 cgcagcacga tggcaaagac ataatccata cagcgggata aggcacaccg ccgtctgagg
     3661 tgcgctttgg gggtttgtaa gggggtattt agcgtataaa taccccctta cgaaataatt
     3721 aaacttttgt cgcgcaacaa aagaaagtat gagaaagaaa catgaatata tatctaacat
     3781 tccctttaaa aaaaagtaat gtctgtcatt ctgaggcgca gacgaagaaa ctcttcgttg
     3841 tataaacaaa aataattcac cactgtcgtc aaaacaacaa taaacagcca tacagggatg
     3901 tatggcgcat ttggatgcgg gcgcatgaga ggtcagcctg ccgtagatga cactacatag
     3961 ccatgtcatt aaaccaggcg ggaaagctcc gactccattt tttcatcact gcgggattaa
     4021 cgaaaaaaca gcgaaggcac ggatgccgta gcgctctacg gctcgtgcga ggacaaagac
     4081 aggatgtct
//
LOCUS       DAOOJH010000161         4080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17074_length_4080_cov_6.692174, whole genome shotgun sequence.
ACCESSION   DAOOJH010000161 DAOOJH010000000
VERSION     DAOOJH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17074_length_4080_cov_6.692174"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..382)
                     /locus_tag="PLJ75_12260"
     CDS             complement(245..382)
                     /locus_tag="PLJ75_12260"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD06029.1"
                     /translation="MACDRELCILFGSGAKGCAKKGSDVDIAIIGDGAKVMHDPFYHI
                     L"
     regulatory      435..570
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            712..2694
                     /locus_tag="PLJ75_12265"
     CDS             712..2694
                     /locus_tag="PLJ75_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPD06030.1"
                     /translation="MLKINNCFISLLFFLSIAPAIYAQDNGSTPDNLTGIIEAVAPST
                     NAQTGEVALDDVIISAYKSNITYAESASVAVITQKDIEKGGYRFVSDVLQSVPGLQLV
                     QSSTLGGTASVFIRGAKTGNMVVLIDGVKVNDISTIERTFDFATLATDSIERIEIIKG
                     PQSALYGSDATGGIINIVTKKGKGKPAVTLNMYAGSYYTTQQSANISGGDSSLNYSLT
                     AGQISAQGYSKAAKPKNAAAPFDDDGFYQRYATGKFGFTPVAPLLIDAGFTVKKSTIE
                     VDDSSYTDDPNSDEERTELSGYTTIKHTIASFWEQSVTVNASRTQREYYDYNDGAWDW
                     PADNTYTGSFYSAEWLQVFSLKNVDTLTVGVNYEKELSHIKDYLNDTSTNNDNSTTGF
                     FAQNHINAGNILYLTASVRADNNEIFGWQQSYTVSPAIVIPKIKTKLKANYGKGYKAP
                     SLYQIYGDGGSIVVENKDLKPEENTGYDIGIEQPLLSIATVNLTYFHNKYKDMIDYDS
                     SSYPGRYININRVTTYGYESSVTITPLKALTLSGGYTYLKTEDETTDKPLMRRPEHQV
                     YSSLTVTIAKFEICATGTYIGKRKDAYYDSATYSTVDVTLDGYFKADIAAHYALTPQL
                     TIHAKAENITDKKYQQTCGYAMPQRSYYVGLTAMVQ"
     gene            2799..3050
                     /locus_tag="PLJ75_12270"
     CDS             2799..3050
                     /locus_tag="PLJ75_12270"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HPD06031.1"
                     /translation="MAKNTIHDRVYKADIIYRLQLDDTDIYLLIKFPLTVDKFMTFRM
                     LTYVMGLYRELIYNHKLTRLPVTFPLVYRGDAQWAVAPS"
     gene            3407..3565
                     /locus_tag="PLJ75_12275"
     CDS             3407..3565
                     /locus_tag="PLJ75_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06032.1"
                     /translation="MHGMTKERLDIAKKMSRNGIDDAIIKISKISPDELQTMKKKMNS
                     DYTIWRKS"
     gene            3562..3918
                     /locus_tag="PLJ75_12280"
     CDS             3562..3918
                     /locus_tag="PLJ75_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06033.1"
                     /translation="MTQYRIITIIIASLIVTAGIGCTNDDTTELAWFNAAGNPINDII
                     WANDDQQWTTENGYADQTKTESKKVNALYGIVIASIDEGSGFREGTVIIEETGSSSLS
                     LHDGSSEVYTISSVNP"
BASE COUNT         1329 a          799 c          838 g         1114 t
ORIGIN      
        1 atcctgattt tattggctat ttttcttcct tctgatgtta tactttcaat gctatccata
       61 ctatcaatcc tccattttgt tattttcttt atctctcaag aaaacaatgt acaaaattta
      121 caatatgagc ggacgctctt ttagcatgat aataaaaaaa tgttcaccag tcaacacatt
      181 aatgtattat tacattttta aacgagcaaa aacagcagta cattcatatt gtgctaaata
      241 attgtcatag aatgtgatag aaggggtcat gcatcacctt tgctccatca ccaatgatag
      301 caatatcaac atcgctgccc tttttggcac atccctttgc accagaacca aataaaatac
      361 ataactccct atcacaagcc aactgcaaaa tatattgaga gtaagcataa aaacgtattg
      421 acaggttata gtaaacatta atcaatggga atcattctac tgaaaaatca gtagacaagg
      481 aggaaggtgg gtgcaaatcc cacgctgacc cgcaactgtg aacccattat attttgggta
      541 agccagatac tcctttagct ggaattcccc gaggattggg aaccagttag tatcaaagca
      601 atttgattct aactaatgta gcatgcctcg ggcatgcttt tttattttta gcgtatcggt
      661 ttccctcctc ctcattccag acatacactt ttaaactgtg aggaggtttc aatgcttaaa
      721 atcaacaatt gttttatttc acttttattt tttttgtcga tagctcctgc catatatgcg
      781 caggacaacg gttcaacccc ggataatctg acgggtatta ttgaggcagt agccccttcc
      841 accaatgcac aaacaggcga ggttgcattg gacgacgtta tcatcagtgc ttacaaaagc
      901 aacatcacct atgctgaaag cgcctcggtt gctgtaataa cacaaaagga tattgaaaag
      961 ggagggtatc gctttgtttc ggatgtgctt cagtcggtgc cggggctaca gcttgttcag
     1021 tcatcaacat tgggtggtac tgcaagcgtt tttatccgtg gtgcaaagac cggcaacatg
     1081 gtggtattga ttgatggcgt aaaggttaat gacatatcaa ctatcgagcg aacatttgac
     1141 tttgctaccc ttgcaaccga ctctattgag cgcattgaaa ttattaaagg tccgcaaagc
     1201 gctctgtatg gttcggatgc aaccggtggg attatcaaca ttgtaacaaa aaaggggaaa
     1261 ggcaaaccag cggtaacact taatatgtat gcaggttcat attacaccac acagcagtcg
     1321 gcaaatataa gcgggggcga tagctcattg aattattctt tgaccgcagg gcagatatca
     1381 gcacagggtt attcaaaagc agcaaagcca aaaaatgctg cagcaccttt tgacgatgat
     1441 ggcttttatc agcggtacgc cacgggaaaa tttggattta cccctgtagc cccattattg
     1501 attgatgctg gatttaccgt aaaaaagtca actattgagg ttgatgactc ttcgtatact
     1561 gatgatccta acagtgatga agagcgtacc gaactttcag gctacacaac catcaagcat
     1621 accattgcat cgttctggga acaaagtgtt acggtgaatg cttcgcgaac gcagcgtgaa
     1681 tactatgatt ataatgatgg cgcctgggat tggccagcag acaatactta caccggctcg
     1741 ttttacagtg cagaatggct gcaggttttc agcctcaaga atgttgatac attgaccgtg
     1801 ggagtaaact atgaaaagga actatcgcac ataaaagatt atttaaatga cacatcaaca
     1861 aacaacgata acagcaccac tggctttttt gcgcaaaacc atatcaatgc aggcaatata
     1921 ctgtacttaa ctgccagtgt cagagccgat aacaatgaaa tttttggctg gcagcaaagc
     1981 tatactgttt cacccgccat cgttatacca aaaataaaaa caaagctcaa agcaaattac
     2041 ggcaagggat acaaagcgcc atccctgtac cagatttatg gcgatggcgg cagtatagta
     2101 gttgaaaata aggacctgaa accggaggaa aataccggtt atgatattgg cattgagcag
     2161 ccattgttat cgatagcaac tgttaatctg acttattttc acaacaaata caaggatatg
     2221 atcgattacg attcttcatc atatccagga aggtatatta acattaacag ggttacaacc
     2281 tatggctacg aaagctcggt aacaataacg ccattgaagg ctctgaccct atctggcgga
     2341 tatacctatc ttaaaacgga ggatgaaaca acggataaac cattgatgcg aaggccagaa
     2401 catcaggtgt atagctcatt aaccgttact atcgccaaat ttgagatatg cgcaaccggc
     2461 acgtacattg gtaaacgaaa agatgcttac tatgacagtg ctacatactc aacggttgat
     2521 gtaacactgg atggttactt taaggctgat attgcagctc actatgctct gacccctcag
     2581 ctaaccatac atgcaaaagc tgaaaatatc accgataaaa agtatcagca aacatgcggc
     2641 tatgcaatgc ctcagcgcag ttactatgtg gggcttacgg ctatggtgca ataaaacttt
     2701 ttatataatt ttttacattt tttctcatgt actttttaat tttttctatc gcaagcgtat
     2761 taatagatag cacataacta tatagtggcg gggcatcgat ggcaaaaaac actatccacg
     2821 acagggtata taaggccgat attatttaca ggcttcagct tgacgatacc gacatatacc
     2881 ttttgattaa attcccgtta acagtggaca agtttatgac ttttaggatg ctcacctatg
     2941 tgatggggct ataccgtgag cttatctata accataagct cacgcgcttg ccggttacct
     3001 tcccattagt gtaccgtggc gatgcgcaat gggcagtagc accatcatga acagtatttt
     3061 tactggaaac cagtgataaa cagcagtttt tcaaaacact tgacaaaata gaagaactgc
     3121 tgtatcgtta taataaagag ccgtatatac tgcggacatt gttgacatgg tttatgcatt
     3181 atttgcacac tcatggtatg attaacacaa taccagagat ataccagaga ttgaagagca
     3241 tttatcaaac gttcaggaag cacaaacgat attatataca tcactgcaaa aaataaatga
     3301 agattataaa agagaaggat atcctgaagg acatcagcaa ggcagagaag aagggattaa
     3361 gaaagggtta aaagcgggta ttacccaggg caaacaacag ggtttagtgc atgggatgac
     3421 aaaagagcgg ttggacattg caaagaagat gagcagaaat ggcattgatg atgcgataat
     3481 aaaaatatca aagataagtc ctgatgaatt acaaacaatg aaaaagaaga tgaactctga
     3541 ctatacaatc tggaggaaat catgacacag taccgtataa taactataat aatagcaagc
     3601 cttatagtga cagctggcat tggctgtact aatgatgaca caactgagct ggcatggttt
     3661 aatgctgctg gtaatccaat aaatgatatt atatgggcaa atgatgacca gcaatggact
     3721 acagaaaacg gctatgctga ccaaactaaa actgagtcaa aaaaggttaa tgcattatat
     3781 ggaattgtta tcgcaagcat cgatgagggc tcaggattcc gggaaggaac ggttattatt
     3841 gaggaaaccg gtagcagttc gctcagtttg catgatggct cttctgaagt atatacaatc
     3901 agttctgtaa atccatgatt ttttttaaaa taagcatatg aacaaaatag tgtcacaatg
     3961 aaacaatatc aaactatcgt acttgtttta acagtgtgcc tgattggcat tggcaccacg
     4021 ctattgcagg cttcggactt tatatggcag caaatatcgg gaaactggtc ggttgttgct
//
LOCUS       DAOOJH010000162         4008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17441_length_4008_cov_8.150266, whole genome shotgun sequence.
ACCESSION   DAOOJH010000162 DAOOJH010000000
VERSION     DAOOJH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17441_length_4008_cov_8.150266"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PLJ75_12285"
     CDS             complement(<1..254)
                     /locus_tag="PLJ75_12285"
                     /inference="COORDINATES: protein motif:HMM:NF015650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheR"
                     /protein_id="HPD06034.1"
                     /translation="MNNIISYQQLNQRDFDYLRNIIYDEAGIKLSDMKKALVQSRLLR
                     RMREMGISSYDEYCHYLRENYDEEIENLINCITTNKTEFF"
     gene            complement(258..821)
                     /locus_tag="PLJ75_12290"
     CDS             complement(258..821)
                     /locus_tag="PLJ75_12290"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06035.1"
                     /translation="MIRTTDATINKEVNILLPGEYYASSDSILSTIVGSCFVVCLYDP
                     LKKLGAMGHCLLPVLSNSQMKKNEIFQYNINFMEHIIGEMVKLGADRKTFVVKVFGGI
                     GKTFNLEYTTDFIKDYCKNENMLLEAIDVGGNYRRKLYYFCKTGKVHKYLVKQNDALS
                     EFITMEKEFIDKVLSDKVQYGNVIIFE"
     gene            complement(858..1349)
                     /locus_tag="PLJ75_12295"
     CDS             complement(858..1349)
                     /locus_tag="PLJ75_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD06036.1"
                     /translation="MNIENTTVENKLDTTSTEQFVTFIIGNETYGIEVLKVQEIIGMT
                     RITYVPNTLPYMKGVINLRGSVVPVIDMRKKIGMDEIAYNAFTVIIITEVKGKLIGMI
                     VDSVQDVVTIPIQKIQDTIHFTSQIATDYIKGIGQIDNNLVIILDVDRLLTADEIAYI
                     KNI"
     gene            complement(1362..1733)
                     /locus_tag="PLJ75_12300"
     CDS             complement(1362..1733)
                     /locus_tag="PLJ75_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD06037.1"
                     /translation="MKYIIIIDDSPTIRTSVEFTLKDIGFGLMHAENGIDALNKIQQL
                     SANGDEVAMCIVDINMPQMDGISFIKKFRENDKFAPVIVLTTEAEEEKIQEGKKAGAS
                     GWMIKPFKAEQLQSVVHKFLR"
     gene            complement(1783..2037)
                     /locus_tag="PLJ75_12305"
     CDS             complement(1783..2037)
                     /locus_tag="PLJ75_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD06038.1"
                     /translation="MYIIEIDEFVTIKHIKQFLDEVKTVLMTEKDIVLDFSKAKRLDL
                     SIVQVILSLLKTAKAEGKTVKFRGVNDTIKKQLKLCGVIR"
     gene            complement(2072..3802)
                     /locus_tag="PLJ75_12310"
     CDS             complement(2072..3802)
                     /locus_tag="PLJ75_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06039.1"
                     /translation="MMNYPVKEQSFKSQHEYLKNLKSNVGSLFSEIIQQFTTINKRID
                     DFFTMIRPYIIYFYTTDEAIADTFLHDVIFSTKDEINGYIETIVKYMIADRETNREVE
                     EAINKTLTLSNSVEMILDAVEAIDLYAKNTMIIAIKAGEEGTTLTTIAVEMSHLADMV
                     NAVSSEFQEIIEQLHSLRTKFSETCMTVDVIVENYLTQLQVRLNNTINDIIQQQKNLS
                     LNVNSIQNFADQIKAVIQKILMEFQIEDIVRQDIEKILFAIEALEESINTDEKEQDVL
                     LWGIYKKLLSLHNDFVHLLDSTVRGINFTRESIVQINNDYNKNAINVYEGDEFAIEKV
                     YNSLEYLQNETIKYIDDILMRKKYLLDISKDVLVRLEKFQEFFRRIQDVVRKFDIVNM
                     LTRIELARHRELQKTIASSLSDISIMPKKIKMIVEESENLYKDVISTMESTYQLYQQA
                     MSIQDEILKNCVNNLKKVSLELYESKKYYVDISQQIERNIHSLQQVLDEKSEQIMAFH
                     EIGKRLKFLCDSMEDMDILKETPELSTIDKIIESINANYGNDYKGLMLISLLKEYSTL
                     QSEDTVIVFK"
     gene            complement(3830..>4008)
                     /locus_tag="PLJ75_12315"
     CDS             complement(3830..>4008)
                     /locus_tag="PLJ75_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPD06040.1"
                     /translation="ESQGRSIGLIVDELIGQQQIVIKSLDNQITTSRAISGAAILGDG
                     TIALIIDVHGFINR"
BASE COUNT         1274 a          772 c          492 g         1470 t
ORIGIN      
        1 ctgaaaaact ctgttttgtt tgttgtaata caattaataa gattttctat ttcttcatca
       61 tagttttcac gcaggtaatg acagtattca tcataactgc taattcccat ctcccgcatc
      121 cttctcaaaa ggcgggattg taccagcgct ttcttcatgt ccgaaagctt tatacctgct
      181 tcatcataga tgatgtttcg caagtaatca aagtcacgct gatttaattg ttgatagctt
      241 attatattgt tcatatgtta ttcaaatata ataacattac catattgcac tttatcggaa
      301 agaaccttat caataaattc tttttccata gtaataaatt ctgataatgc atcattctgt
      361 tttaccagat atttatgcac cttccctgtt ttgcaaaaat aatataattt ccttctatag
      421 ttaccaccta catcaattgc ttcaagcagc atattttcat ttttacaata gtctttaata
      481 aaatcggttg tatactcaag attaaacgtt ttaccaatgc cgccaaaaac tttaaccaca
      541 aaggtttttc tatcagcgcc taattttacc atttctccta taatatgttc cataaaatta
      601 atattatatt gaaatatctc attttttttc atctgcgaat tagataaaac aggcaataag
      661 caatgaccca ttgcaccaag tttttttaag ggatcatata aacaaacaac aaaacaactc
      721 ccaacaatag tggaaagtat tgaatcggat gatgcataat attcacccgg caacaaaata
      781 tttacttctt tgtttatagt tgcatcagta gttcggatca taatgtttac aatatatcat
      841 tttgatatac attgatatca aatattttta atatatgcta tctcatctgc agttaatagc
      901 ctgtcaacat ccagaataat aactaaatta ttatcaatct gcccaatacc ctttatataa
      961 tcagttgcta tctgtgaagt aaagtgaatg gtatcctgaa tcttctggat aggtatagta
     1021 acaacatcct gcaccgaatc aacaatcatt cctatcagtt tccccttaac ttcagtgata
     1081 ataattactg taaaggcatt atatgcaatt tcatccattc caattttttt acgcatatca
     1141 atgacaggaa ccactgaacc ccgtaaatta ataactcctt tcatataggg taacgtattg
     1201 ggtacataag taatacgcgt cataccaata atttcctgta cctttaacac ttcaatacca
     1261 tacgtttcat ttcctataat gaacgtaaca aactgttctg ttgatgtagt atctaattta
     1321 ttttcaaccg tagtattttc aatattcata aagttgcctc cttaacgtaa aaatttatgg
     1381 actacagatt gtaattgctc tgctttaaat ggctttatca tccaccccga ggcaccagcc
     1441 ttcttccctt cctgtatctt ctcttcttct gcttcagtgg taagcacaat aacaggagca
     1501 aatttgtcat tttcccggaa ctttttaata aaactaattc catccatctg aggcatgttt
     1561 atatcaacaa tacacattgc cacttcatcg ccatttgccg atagctgttg tattttattc
     1621 aacgcatcaa taccattttc agcatgcatc agtccaaagc caatatcttt tagagtaaat
     1681 tcaacgcttg ttctgatggt aggcgaatca tcaataataa ttatatactt catactatac
     1741 cctcatgtaa tatattcatt atgtataatg taatcaaatt aattaacgaa tgacaccaca
     1801 caattttaac tgctttttga tagtatcatt gactcctcta aatttaaccg ttttcccttc
     1861 agcttttgct gttttcaaaa gcgataatat tacctgtact attgataaat ctaaacgctt
     1921 tgctttagaa aaatcaagga caatatcttt ttctgtcatc aagacagtct ttacttcatc
     1981 cagaaattgt tttatgtgct ttatagtgac aaattcatct atttcaatga tatacatcgt
     2041 tattacctcc tacaaacaca tactttatac gttatttaaa gacaatcaca gtatcttctg
     2101 attgtaaagt tgaatactcc tttaacaggg aaatcagcat taaaccttta taatcgttac
     2161 cataatttgc attgatactc tctataattt tatcaatagt ggacaactca ggtgtttctt
     2221 ttaaaatgtc catatcttcc atgctatcac acaaaaattt caaccttttt ccaatttcat
     2281 gaaatgccat aatttgctct gacttttcat caaggacctg ttgcaatgaa tgtatattcc
     2341 tttctatctg ttgggatata tcaacataat atttctttga ttcgtacagt tccagagata
     2401 ctttttttaa attattcacg cagtttttta aaatttcatc ctgtattgac atcgcctgct
     2461 gatataactg ataagttgat tccatagtac ttattacatc tttatataaa ttttcagact
     2521 cttcaacaat cattttaatc ttttttggca taatgctgat atcagaaagt gaactggcaa
     2581 tggttttttg taactctcta tgacgtgcta attcaatacg cgttaacata ttgacaatat
     2641 caaattttct tactacatcc tgtattcttc tgaaaaattc ctgaaacttt tccagtcgta
     2701 ccagcacatc ctttgagata tcaagaagat actttttcct cattaatatg tcatctatat
     2761 atttgattgt ctcattttgt aaatattcca gtgagttata aactttttca attgcaaatt
     2821 catctccctc ataaacattt attgcatttt tattataatc attatttatt tgtacaatgc
     2881 tttctcttgt aaaattgatc cctctcacag tactatcaag caaatgaaca aaatcattat
     2941 gaagtgataa taactttttg taaatccccc ataataaaac atcctgttct ttttcatctg
     3001 tattaattga ttcttcaagc gcttcaatag caaagagtat tttttcaata tcctgacgaa
     3061 caatatcttc aatttgaaat tccattaaga ttttctgaat cactgctttt atctgatcag
     3121 caaagttctg aatggaattg acatttaatg acagattttt ttgctgttga atgatatcat
     3181 ttatagtatt atttaatcta acctgtaatt gagtcagata gttctctaca ataacatcaa
     3241 cagtcatgca tgtttcactg aattttgtgc gcaatgaatg aagttgttca ataatttcct
     3301 gaaattcact ggaaacagca tttaccatat cagctaaatg tgacatctca acagctattg
     3361 tagttaatgt tgtgccttct tccccggctt taattgctat gatcatggta ttcttagcat
     3421 ataaatctat agcctcaaca gcatctaaaa tcatttcaac actgttgctt aaagtgagtg
     3481 ttttattgat agcctcttca acttccctat tggtttccct atcagctatc atatatttaa
     3541 caatagtttc gatgtaacca ttaatctcat ccttagtact aaaaataaca tcatgcaaaa
     3601 atgtatcagc aatagcttca tccgttgtat aaaaatagat gatatatggc cgtatcattg
     3661 taaaaaaatc atctattcgt ttattgatgg ttgtaaactg ctgaattatc tcagaaaata
     3721 atgaaccaac attactttta agatttttaa gatattcatg ctggctttta aaagattgtt
     3781 cttttacagg atagttcatc atagattcct tatggtactc aatggcatat tacctgttaa
     3841 taaatccatg aacatctatt atcagtgcga tagttccatc acccaatatc gcagcaccag
     3901 aaatagccct ggaagttgtg atctgattat caaggctttt aatgacaatc tgctgctgac
     3961 caatcagctc atcaactata agcccaattg atctgccctg agactcaa
//
LOCUS       DAOOJH010000163         3947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17773_length_3947_cov_9.506937, whole genome shotgun sequence.
ACCESSION   DAOOJH010000163 DAOOJH010000000
VERSION     DAOOJH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17773_length_3947_cov_9.506937"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLJ75_12320"
     CDS             <1..747
                     /locus_tag="PLJ75_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD06041.1"
                     /translation="YIGWNAKNPLFNNKNVRKALTMLINRQAILDSLLFGLGTVVTGP
                     FYIYDKYYNHDIQPLPYDTEKAKELLNSAGWVDSDGDGILDKGGTKFSFTFTIASASK
                     FAERLATILKEDFAKVGIQMDINRYEWAVFVNKIDKRDFDAVTLGWSLSWEGDPYQLW
                     HSSEVKAGSNFCYFINREADEIIEKARKEFDVNKRIALYRRFHEIINDEQPYTFLYCT
                     PALVAVSKRFTNVIVHKRGLNYVEWKVTGN"
     gene            747..1748
                     /locus_tag="PLJ75_12325"
     CDS             747..1748
                     /locus_tag="PLJ75_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD06042.1"
                     /translation="MRYYIIKRILIIIPTFIGITFITYLMIRLAPGDFTSLRAGIEGE
                     LKAGSLSKEIFEQEKKLYGLDKPIIVGYSEWLYKTVKLDFGTSRKDGRTVAAHIIESL
                     PITLSLNILSIVIVYIISIPLGIFSAIKKDTVIDRIVSITLFLLYSLPSFWVALLLLK
                     YLSGGDYLNLFPLGGLYSDWFDQLNVLQKAMDVIWHLILPVVTLTYGGFAFLSRYTRA
                     TMLEVINQQYIVTARAKGLSEKKVLFVHAFRNSLIPLITLMATMLPGLLGGSVVVESI
                     FSVPGMGMLAFESILSRDIPVIMAITSISAFLTLIGIFLGDIMYAIVDPRIRLEAQQ"
     gene            1745..>2214
                     /locus_tag="PLJ75_12330"
     CDS             1745..>2214
                     /locus_tag="PLJ75_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD06043.1"
                     /translation="MTGYWGTAWKRFKHNKVALGGLWIVLFLFVIALCAPLIANNKPY
                     IIIKNNALYFPIIIDYKEFEGVDFKKIDGYKLFPPIPYSYSEYDLNAIVVPPSSKHLL
                     GTDEQGRDLAARMIYGTRVSIFVGFVAVFIYVTIGIIIGALAGFYGGWVDIIISR"
     assembly_gap    2215..2314
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2315..2572
                     /locus_tag="PLJ75_12335"
     CDS             <2315..2572
                     /locus_tag="PLJ75_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD06044.1"
                     /translation="MAPVMVSATFGIASTILIESSLSFLGFGVQPPTPSWGDILSQSR
                     DFIDFAWWLTLIPGFAIFITITSYNVVGEGLQDALDPKQYY"
     gene            2544..3437
                     /locus_tag="PLJ75_12340"
     CDS             2544..3437
                     /locus_tag="PLJ75_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06045.1"
                     /translation="MHLIPNNIIKGFVFIFIISLFSCLSMFEEQQNTANLLPSDTELY
                     TWKLVKSKTINDQQSITMLVPSFYQHYNVIELVTGKYESINDPAISLTVTIARTSNAD
                     DAYGLFTRERALLNDTFEIDTVTAYSKNKSIAYIDNIIIIVQTNDIDFIKTQKTTISI
                     ILDKVRSNQQLPDVMNPVTKLINESNIVIYNEGIPELPDIKHIIVGNKTIDSSEISCF
                     YKKYFSSLDAGNSFNSLIKNDSSLMIVESSKVKIAFKKYGDSYCYVAHSNEWIFGCKD
                     IKDYATGKKVIAVIYRELVAQ"
     gene            3439..>3947
                     /locus_tag="PLJ75_12345"
     CDS             3439..>3947
                     /locus_tag="PLJ75_12345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPD06046.1"
                     /translation="MFYLIGDIHGHFQKLSHLVDKIKKYLTLDDSLVFLGDYIDRGYQ
                     SFEVIDYLVSLKDEYNTIILKGNHEGMFLNYLQGYDISIFMYNGGHATIQSYEETIGS
                     LKLPQAHKNFFNSLKLYYEADTFIAVHAGLRPGISLQQQSEHDLLWIREEFYQKQYTW
                     DKTVIFGHTA"
BASE COUNT         1171 a          621 c          781 g         1274 t
ORIGIN      
        1 tatattggct ggaatgcaaa aaatcccctg tttaacaata aaaatgttcg caaagctctc
       61 accatgctca ttaataggca ggctatactg gatagcttac tttttgggct gggtacggtt
      121 gttaccggtc cattttacat ctatgataag tattacaacc atgatataca accactgccc
      181 tatgatacgg aaaaggcaaa agaattgctt aacagtgcag gctgggtgga tagtgatggc
      241 gatggcatac tggataaagg tggcacaaag ttttcgttta cttttacaat agcatcagca
      301 agtaagtttg ctgaacggct tgcaacaatt ttaaaagagg attttgcaaa ggttggcatt
      361 caaatggata tcaaccgata tgaatgggct gtgtttgtca ataagattga caaacgcgat
      421 tttgatgctg ttacattggg gtggtcatta tcatgggagg gcgatccgta tcaactgtgg
      481 cattcttcgg aggttaaagc aggttcaaat ttttgttatt tcataaatcg agaagctgat
      541 gagataattg agaaggctcg aaaagagttt gatgtaaaca agcgcattgc actataccgc
      601 cgttttcatg aaataatcaa tgatgagcag ccctatacct ttttatactg tacgccggcg
      661 cttgttgcgg tgagcaagcg ctttaccaat gttattgttc ataaacgtgg gttaaactat
      721 gtagagtgga aggttacagg caattaatga gatactatat catcaaacgt atactaatta
      781 ttataccaac ctttattggt attaccttca tcacctatct tatgatacgg ttggcaccgg
      841 gtgactttac atcattacgt gcgggaatag aaggagagct taaggcgggg agcctgtcca
      901 aggagatatt tgaacaggaa aagaaactgt atggattgga caaacctatt atagttggat
      961 attcagaatg gctttataaa acagtaaaac ttgattttgg cacctctcga aaagatggac
     1021 gtacagtagc agcacatatt attgagtcat tgcctattac attatctctt aatattttgt
     1081 cgatagttat tgtttatatt atttcaattc cattaggtat tttttcagca ataaaaaaag
     1141 atactgtgat tgatcgcatt gtcagcatta cactattttt actatattct ctgccgtcct
     1201 tctgggttgc attgcttctt ttaaaatatt taagcggtgg cgattatttg aaccttttcc
     1261 cgcttggagg cttatactct gactggtttg atcaattaaa tgtattgcaa aaagcaatgg
     1321 atgttatatg gcatttgatt ttaccggtgg ttaccctgac ctatggtgga tttgccttcc
     1381 tttcacggta tacccgtgca acaatgcttg aggtaataaa tcagcaatat atagtaacag
     1441 cacgtgcaaa agggctgtcc gaaaaaaagg ttttatttgt gcatgccttc agaaattctt
     1501 taattccgct tattacgttg atggcaacaa tgctgccggg gcttttaggt ggcagtgtgg
     1561 tggttgaatc tatattctcg gtaccgggta tgggtatgct tgcgtttgaa tctatcctat
     1621 cacgggatat tcctgttatt atggctataa catccatttc agcattcctc acacttattg
     1681 gaattttcct tggggacatc atgtatgcga ttgttgatcc aagaatcagg ctggaggcgc
     1741 aacaatgacg ggttactggg gtacagcatg gaaacgcttt aaacacaata aggttgcact
     1801 tggtggcttg tggatagtgt tgtttttatt tgttattgca ctgtgtgctc ctttgattgc
     1861 aaacaataag ccctacatca ttataaaaaa taatgcacta tattttccca ttatcattga
     1921 ttataaagaa tttgaaggag ttgattttaa aaagatagat gggtataaac tgtttccgcc
     1981 aattccctat tcgtactctg aatatgatct caatgccatt gttgtgcctc cttcgagtaa
     2041 gcacctgctg gggactgatg agcagggtag ggatttagct gcacgtatga tctatggcac
     2101 aagggtatct atttttgttg gatttgttgc agtgtttatt tatgttacca ttggtatcat
     2161 tataggtgca ctggctgggt tttatggagg atgggtggat attattattt cccgnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggct cccgtgatgg tttcggcaac
     2341 atttggcata gcctcaacaa tacttataga atcatcgttg agctttttag gatttggggt
     2401 acaaccacca acgccaagct ggggtgatat attatcccaa tcacgtgatt ttattgactt
     2461 tgcatggtgg ttgacattaa tcccgggttt tgctatattc attactataa catcatataa
     2521 tgtagtaggt gagggtttac aggatgcact tgatcccaaa caatattatt aaaggttttg
     2581 tttttatttt tatcatcagt cttttttcat gtctttcgat gtttgaagaa cagcagaaca
     2641 cagcaaacct gttaccatca gatacagagc tgtatacatg gaagcttgta aaaagtaaaa
     2701 ccattaatga tcagcagtca ataacaatgc tggtaccatc gttttaccag cattataatg
     2761 tcatagagtt ggttaccggc aaatatgaat caattaatga ccctgccatt tctttaacag
     2821 taactatcgc cagaacatca aatgctgatg atgcatatgg actgtttaca cgtgagcgtg
     2881 ctcttttaaa tgatacgttt gaaatcgata ctgttacagc gtatagtaaa aataaaagta
     2941 ttgcctatat cgataatatt attattatag ttcagaccaa tgacatagat ttcatcaaaa
     3001 cgcaaaaaac tactattagc ataatacttg ataaagttag atcaaatcaa caattacccg
     3061 atgtaatgaa ccctgtaaca aagcttatca atgaaagcaa tattgtcatt tataatgaag
     3121 gcattccgga attaccagat attaaacata tcattgttgg caataagact attgattctt
     3181 ctgagatatc atgtttttat aaaaaatatt tttcaagtct tgatgcaggc aatagcttta
     3241 attcattgat aaaaaatgat agttcattga tgattgttga atcaagcaag gtaaaaatag
     3301 cttttaaaaa atatggtgat agttattgtt atgtagcaca tagtaatgaa tggatatttg
     3361 gctgtaaaga tattaaagat tatgctaccg gtaaaaaagt aatagctgtt atttataggg
     3421 aactggttgc tcaataaaat gttttacctc attggtgata ttcatgggca cttccaaaaa
     3481 ttgtcccatc ttgttgataa aatcaaaaaa tatttaaccc ttgatgatag tctcgttttt
     3541 ctgggagatt acattgatcg cggttaccaa tcatttgagg ttattgatta tctggtaagt
     3601 ttgaaagatg agtacaatac aattatttta aaagggaatc atgaaggcat gtttttaaat
     3661 tatctacaag ggtatgatat atcgatattc atgtataatg gggggcatgc aacaatacaa
     3721 tcatatgaag aaactattgg ttctttaaag ttgccgcaag cccataaaaa cttttttaat
     3781 agtcttaaac tgtattatga agctgatact tttattgctg tacatgctgg tttacggcca
     3841 gggatatcat tgcagcagca atctgagcat gaccttttgt ggatacgaga ggaattttat
     3901 caaaaacaat atacatggga taaaacggtt atttttggac atactgc
//
LOCUS       DAOOJH010000164         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17811_length_3940_cov_8.746976, whole genome shotgun sequence.
ACCESSION   DAOOJH010000164 DAOOJH010000000
VERSION     DAOOJH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17811_length_3940_cov_8.746976"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PLJ75_12350"
     CDS             <1..707
                     /locus_tag="PLJ75_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06047.1"
                     /translation="RQFVKSCAATLPLPRFYSDNYRYVAMAQRIATSSSFVGYCSCCM
                     YSKGTALGHSFYHSQKVAIEAAAIVLKEKGISEHSVHLAVLALMAGYFHDYFREMNDH
                     PSKAAEYVQNHFYHFIPENDVLAISFAIQNHEAFKEHQQVSNDDIMLLSNALYDADKF
                     RWGPDNFLYTIWDMIVTMHISHNDIVVHFSKGVAYIDTIRHTFRSKTGIQYGPEFIDQ
                     GLVIADQLKNYLETTE"
     gene            988..1060
                     /locus_tag="PLJ75_12355"
     tRNA            988..1060
                     /locus_tag="PLJ75_12355"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1021..1023,aa:Thr,seq:ggt)
     gene            1234..1386
                     /gene="rpmG"
                     /locus_tag="PLJ75_12360"
     CDS             1234..1386
                     /gene="rpmG"
                     /locus_tag="PLJ75_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPD06048.1"
                     /translation="MREVILLSCKDCKNRNYATKKDKKKQTGKLEVMKFCKHCNKHTL
                     HKETKA"
     gene            1398..1583
                     /gene="secE"
                     /locus_tag="PLJ75_12365"
     CDS             1398..1583
                     /gene="secE"
                     /locus_tag="PLJ75_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPD06049.1"
                     /translation="MFNKTIQFVKESRDELKKVSWPERDEVSALTMVVLITVIITSLF
                     LWLVDATLMKIVSLVMK"
     gene            1586..2128
                     /gene="nusG"
                     /locus_tag="PLJ75_12370"
     CDS             1586..2128
                     /gene="nusG"
                     /locus_tag="PLJ75_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009710882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPD06050.1"
                     /translation="MVKGWYVIHTYSGHENKVKLALEKKVQNLNLADKIFQVKIPTVE
                     EHSVKDGKKVVKSKKIFPGYVLVEMIIDDETWMAIKNIPGVTNFVGSFGSEKPRPLSE
                     KEVDNLFNKMGEVATKDKVSVVMDFSVGEHVKVIDGPFKEFTGVIEEVYPEKGRLRVK
                     VEIFGRGTPVELDFVQVGKI"
     gene            2155..2589
                     /gene="rplK"
                     /locus_tag="PLJ75_12375"
     CDS             2155..2589
                     /gene="rplK"
                     /locus_tag="PLJ75_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPD06051.1"
                     /translation="MAPKKKKIVTQIKLQIPGGQANPAPPVGPALGQHGLNIMEFCKA
                     FNDRTKDQQGTILPVVITVYEDRTYTFIVKTPPAAVLIKKALKLEKGSSEPNKKKVGT
                     ITQSQLEEIAKLKMPDLNANDVEAAKKIIAGTARSMGVEVSG"
     gene            2635..3318
                     /gene="rplA"
                     /locus_tag="PLJ75_12380"
     CDS             2635..3318
                     /gene="rplA"
                     /locus_tag="PLJ75_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPD06052.1"
                     /translation="MKRGKKITDAKAKVDKTKLYPIDEALSLMKELAFVKFDETVEVA
                     IKVIHKSYQNVRGSVVLPHGTGKEIKVLVICKGDKQKEALEAGADFVGAEDVIEKIKN
                     GWLDFEAVVATPDMMKEVGKLGPILGKKGLMPKPKSGTVTDDVKTAVKELKGGRVEYK
                     ADKTGVIHLGVGKLSFEQQKLAENIKSFYGQVVKDKPSDAKGNYIRSFHICSTMGPGI
                     KINYKGIER"
     gene            3318..3833
                     /gene="rplJ"
                     /locus_tag="PLJ75_12385"
     CDS             3318..3833
                     /gene="rplJ"
                     /locus_tag="PLJ75_12385"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPD06053.1"
                     /translation="MVKQYKIDSVNELKEKLNSKKNIILTHYSGIKVKDLYQLRKILR
                     DKGAEYKVIKNNLFRKALKDSGYPPIDTHLTGPIGVVFVDKELSEIAKVLKDFQKEQD
                     KFQFSVAIMDNVVYSQKDVQRIAELPPKEVLLSQVMSLINGPATGTAIAINQVMASLA
                     RGIKLVAQQNA"
BASE COUNT         1348 a          563 c          857 g         1172 t
ORIGIN      
        1 gcaggcagtt tgttaagtca tgtgctgcaa cattgcctct accccgtttc tatagcgata
       61 attaccggta tgtagctatg gcacaaagga tagcaacctc atcgtcgttt gtggggtatt
      121 gctcttgttg catgtatagc aaaggtacag cattggggca tagcttctac cattcacaaa
      181 aagttgctat tgaagcagca gcgatagttc ttaaagaaaa aggtatttct gaacattcgg
      241 tacatttagc agtattggcg ctcatggcag gatatttcca tgattatttc cgtgagatga
      301 acgatcatcc gtcaaaagct gctgagtatg tacagaatca tttttaccat tttattccgg
      361 agaatgatgt gctggcaatc tcgtttgcca tacaaaacca tgaagcattt aaagagcatc
      421 aacaggtgag caatgacgat ataatgcttt tatccaatgc actgtacgat gctgataaat
      481 tcaggtgggg tccggataat tttttataca caatatggga tatgatagtc acgatgcata
      541 tttcgcacaa tgatatagta gtacattttt caaaaggagt ggcatatatt gatacaatac
      601 gccatacatt caggtcaaaa actggtatac agtatggacc tgaatttatt gatcaggggc
      661 tggtcattgc agatcaatta aaaaattatt tagaaacaac agagtagtga atgttgttga
      721 taacatatgc tgtacctatt gatacatgcc atatatcctt accatatact acgataagta
      781 taataggtac aattgcaact atgttttttc tttgacagta actattgaaa agatgtatca
      841 tttgtattat attataaaaa accatgctct atataataac aaaaggcgtg agcaaaactc
      901 agcagtatat taataaaaaa cttgacaaaa taccatattt ttcagggata cttaacgaaa
      961 gtagtatatg atgtttattt caggtttgcc cacgtagctc agtcggtaga gcatttgcat
     1021 ggtaagcaaa aggtcaccag ttcgattctg gtcgtgggct attttgttat gtattaaaaa
     1081 atttgtattt tttttaattt gagcaattaa ttgcttgacg tgatgtgcta tatttatagc
     1141 gtgctttcat atctttaaaa agcatacaca ttttcagcaa ctgttaaaca ttacataacg
     1201 caaaaaatac tattacatta acaggatgtc attatgcgtg aagtgatact attatcatgt
     1261 aaggattgca aaaatcgcaa ttatgccaca aagaaggata agaaaaagca aaccggcaag
     1321 cttgaagtaa tgaaattttg caagcattgt aacaagcata cattgcataa ggaaacgaaa
     1381 gcataaggtg ggcgatagtg tttaataaaa ccatacaatt tgttaaagaa tcacgtgatg
     1441 agcttaaaaa agtttcctgg cctgaaaggg atgaggttag tgcattgacc atggtggttc
     1501 ttataactgt tataattact tcactttttt tatggcttgt tgatgctacc ttgatgaaaa
     1561 ttgtttccct ggtgatgaaa taactatggt aaaaggttgg tatgtaatac atacttattc
     1621 gggtcatgag aataaggtta agctggcttt ggaaaagaag gtacaaaatc tgaatttagc
     1681 tgataagata tttcaggtta agattccaac tgttgaagaa cattcagtta aagatgggaa
     1741 aaaggttgta aaatctaaaa aaatatttcc aggatatgtt cttgttgaaa tgattattga
     1801 tgatgaaacg tggatggcaa taaaaaacat tcctggagtt acaaattttg taggttcatt
     1861 tggtagcgag aaaccacggc cattatctga aaaagaggtg gacaacctgt tcaataaaat
     1921 gggtgaggtt gccactaaag ataaggtgtc ggttgttatg gatttttcgg taggagagca
     1981 cgtaaaggtt attgatggtc cgtttaaaga atttaccggt gttattgaag aggtatatcc
     2041 agagaagggc aggttgcgtg ttaaggttga aatatttggc agggggacgc ctgttgaatt
     2101 ggactttgtg caggtaggta agatataata atagttatat aagggttgat gacaatggct
     2161 ccaaaaaaga agaaaatagt aactcagatt aaattacaga ttcctggtgg acaggcaaat
     2221 cctgcaccac cggtagggcc tgctcttgga cagcatgggc ttaacatcat ggaattttgt
     2281 aaggctttta acgatagaac aaaggatcag cagggtacca tactgccggt agttattact
     2341 gtatatgagg acagaacgta tacgttcatt gttaaaacac cgcctgcagc agtattgatt
     2401 aaaaaggctc ttaagttaga aaaaggttca agcgaaccta ataagaagaa ggttggcacc
     2461 attacacagt ctcaacttga agaaattgct aaattaaaaa tgccggatct caatgctaat
     2521 gatgttgagg cagcaaagaa aatcattgct ggtacggcgc gttcaatggg agttgaggta
     2581 agtggataac ataattaata taattaatta ataatgaatt atttggggtt ttttatgaag
     2641 cgaggaaaaa aaattactga tgcaaaggct aaggttgata agaccaaatt atacccgatt
     2701 gatgaagcat tgtcattaat gaaagaatta gcctttgtaa agtttgatga aacagttgag
     2761 gttgcaataa aagttatcca taaaagctat caaaatgtac gtggttccgt ggtattaccg
     2821 catggcactg gtaaagaaat aaaagtgctt gtgatttgca aaggtgataa gcaaaaagaa
     2881 gctttagaag caggagctga ttttgtaggt gcagaagatg ttatcgagaa gataaagaat
     2941 gggtggcttg attttgaggc ggttgttgca acccctgata tgatgaaaga ggtaggaaag
     3001 ttaggcccta tattgggtaa aaaagggctt atgccaaaac ctaaatctgg caccgtcact
     3061 gatgatgtaa aaacagccgt taaagagctt aagggtggaa gggttgaata caaggccgat
     3121 aagaccgggg ttatccatct tggtgttggt aaactttcat ttgagcaaca aaagcttgcg
     3181 gaaaacatta aatcgttcta tggccaggtt gttaaagata aaccctctga tgcaaaagga
     3241 aactatattc gttcttttca tatatgctct acaatgggac caggcattaa aattaattac
     3301 aaggggatag aacggtaatg gttaaacaat ataaaataga ttctgttaat gaattaaaag
     3361 aaaaactgaa ttcaaaaaag aatattatat taacacatta ttccggtatc aaggtaaaag
     3421 atctttatca attgaggaag attttgcggg ataaaggtgc tgagtataaa gtaattaaaa
     3481 ataatttatt cagaaaagca ttgaaggata gcggatatcc cccaatagat acacatctga
     3541 caggtcctat tggtgtggtg tttgttgata aggaattgag tgaaatagca aaggtattaa
     3601 aggattttca gaaagagcag gataaatttc agtttagcgt agcaataatg gataatgttg
     3661 tatattctca aaaggatgta caacgaattg cagaattacc ccccaaagag gtgttattgt
     3721 cgcaggtaat gtcgcttatc aatgggcctg ctacagggac agctattgca ataaatcaag
     3781 ttatggcttc ccttgccagg ggtattaagc ttgtagcaca gcaaaatgct tagcttgtta
     3841 tagcatttat ataatcaagg gtacataata cttatcagaa ttgtaaacat tatggagtat
     3901 tggttgccct tataagtttt tacgacaaat gttcgtatta
//
LOCUS       DAOOJH010000165         3935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_17842_length_3935_cov_9.475515, whole genome shotgun sequence.
ACCESSION   DAOOJH010000165 DAOOJH010000000
VERSION     DAOOJH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17842_length_3935_cov_9.475515"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLJ75_12390"
     CDS             <1..217
                     /locus_tag="PLJ75_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06054.1"
                     /translation="KYSSKAEKGLSVRVNGQQISLKGIGAGLKIMCAFMDSITIDLID
                     KEKMISDTVSKVTDGTILSMHRKRRYK"
     gene            217..957
                     /locus_tag="PLJ75_12395"
     CDS             217..957
                     /locus_tag="PLJ75_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPD06055.1"
                     /translation="MRPEKAYKNMEFLNSREARIIRIMCEYIEPEKRFKHYKIKHTVV
                     FFGSARTQPDDPLTMQYYHDACKLSYLLAQYGKELEAVHDGFVICTGGGPGIMEAANK
                     GANEAGVYSIGLNISLPFEQIPNQYISQELNFEFHYFFMRKLWFLYHAKAIVVFPGGY
                     GTLDELFETLTLVQTNKLQKQDIPIVLYDENYWNKLINFQMLIDCGYIDKKDFDLIHF
                     CNSPEEGIEYLKPRLKGLIEKINHSIGE"
     gene            964..2160
                     /gene="argJ"
                     /locus_tag="PLJ75_12400"
     CDS             964..2160
                     /gene="argJ"
                     /locus_tag="PLJ75_12400"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPD06056.1"
                     /translation="MEKIKELSGGLENVTIFTYAAIECGIRYENRLDFSLIYSDKPCW
                     AAGAFTTNKFCAAPVKLCRERIKNTIHAVIINATNANAATGKQGYDNAKLLTAEVAKI
                     LSIDEKTVLMASTGIIGVQLPVEKMKNNLPILVKNLNSESGKLLPTAIMTTDTFPKQY
                     ALSFETSTGIYTIAGIAKGAGMIAPDMATLLCFVVTDFPLQQRVLQNLFSASIAVSLN
                     AITIDGDMSTNDTAILLSPVLNRKHTHDDIEIFNNALTMMMKRLAYLIVSDAEGATKC
                     VTIKVVGAANDSDAKIAAKSIAESLLVKTAIFGNDPNWGRIACAAGYSGAELREDKLS
                     IYFEDISLFKNGSPVNFDKDRLIDILKRKEYTIIIDFGIGKSLFEYLTSDLTYEYVKI
                     NAEYST"
     gene            2303..3385
                     /locus_tag="PLJ75_12405"
     CDS             2303..3385
                     /locus_tag="PLJ75_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06057.1"
                     /translation="MAEFKRIVYSIIMFFIFISLVNGQTIDKHKIIIYYFEDESSLQN
                     YRYYSYIIPDNLAIELQNHVEYEVTVLPVTFKYISDDMPQEEKENTITILSTRAKEVN
                     ARFVIIGSYKVEKKRIFIQTQIYNTVTGELTDAAEVEETIGAIVYEIIEKITLKINSQ
                     LQKTLDVEKEKIALSPYKDFYSTFQNSYFGYSYSYVFFTGSWENLYNDPKMSSVYIGF
                     PLSKMYASNSVFIKNIYIVPTWQFYSTNNHDLKNMNDSYLSIYTGSIALQYQYSLSPF
                     MIVFASLGAGVSYSDMRFVLASEGGPFSPETKTDSTDPYFGANLGAIFYIHNIGISGG
                     LGYNRIFYKGDDLASFVMSFGILYRL"
     gene            3511..>3935
                     /locus_tag="PLJ75_12410"
     CDS             3511..>3935
                     /locus_tag="PLJ75_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06058.1"
                     /translation="MKSKIVYSHNDYWCKHQIYDAIAYGCDMVEADVIYMNNKIILSH
                     SWRPFSFMCYGNLEDNYLKPLSQYCKDNPDKTMWMYVEFKDSNEHIIELLHKLFKQYQ
                     TKNLRYTVSALGSKWYQKKRYETAMQFYKKYKDEFSLLW"
BASE COUNT         1389 a          574 c          688 g         1284 t
ORIGIN      
        1 aaagtattca tctaaagcgg aaaaaggact cagcgtgcga gtaaatggcc agcagatttc
       61 cttaaaagga attggtgctg gtttaaagat aatgtgtgct tttatggact caattaccat
      121 cgatcttatt gacaaagaaa aaatgatttc tgatactgtc agcaaagtta ctgatggtac
      181 tatcttatca atgcacagga agaggagata caaataatga gaccggaaaa agcatataaa
      241 aatatggaat tcctcaattc acgagaagca cgcattatca ggataatgtg cgaatatatt
      301 gaacctgaaa aaaggtttaa acattataaa ataaaacata ccgttgtttt ttttggttct
      361 gctcgtaccc aaccggatga tcctttaacc atgcaatatt atcatgatgc ctgcaaactt
      421 tcatatttgc tggcacaata tggaaaagaa ttggaggcag ttcatgacgg ttttgttata
      481 tgtacaggcg gtggtcctgg tataatggaa gcagcaaaca aaggagctaa tgaagctgga
      541 gtatattcaa tagggttgaa tatatcactc ccttttgagc aaatacccaa tcaatatata
      601 tctcaagaac taaatttcga atttcattac tttttcatgc gaaaattatg gtttctttat
      661 catgcaaaag ctattgtagt attcccgggc ggttatggca cacttgatga actatttgaa
      721 acattgactc tggttcaaac taataaatta caaaaacagg acattccaat agttttatat
      781 gatgagaatt attggaataa gttaattaat tttcagatgt tgattgattg tggttatata
      841 gataaaaaag attttgatct aatccatttt tgtaattccc ctgaagaagg tattgaatat
      901 ttaaaaccac gtttaaaagg attaatagaa aaaattaacc attcaattgg tgagtaatga
      961 atcatggaaa agattaaaga gctatctggt ggtcttgaaa atgtaaccat atttacatat
     1021 gctgcaatag aatgtggcat tcggtatgaa aatagattgg atttttcatt gatatattct
     1081 gataaaccat gttgggccgc tggtgcattt actaccaata aattttgcgc tgcaccagtt
     1141 aaactgtgca gagaaagaat aaaaaataca attcatgcag ttatcataaa tgctactaat
     1201 gctaatgcgg caactggtaa acagggctat gataatgcta agctactaac tgctgaagta
     1261 gcaaaaattt tatcaataga tgaaaagact gttttaatgg catcaacggg tattattgga
     1321 gtgcaattac cggttgaaaa aatgaaaaat aacctgccca tattggtaaa gaatttaaat
     1381 agcgagtcag gaaaactgct tccaactgct ataatgacaa ccgatacttt cccaaaacaa
     1441 tatgcattat cctttgaaac atcaacaggt atatatacta ttgcaggtat tgccaaggga
     1501 gcggggatga tagctccgga catggcaacc cttttatgtt ttgttgtaac agattttcca
     1561 ttgcagcaaa gggtattaca aaatttgttt tctgcaagta ttgctgtttc attgaatgct
     1621 ataacaatag atggtgatat gtcaaccaac gatacagcta ttttgttatc tcctgtactc
     1681 aacagaaaac atactcatga cgatatagaa atctttaaca acgcactcac tatgatgatg
     1741 aaacgattgg cataccttat tgtcagtgat gcggaaggag caacaaaatg tgttacgata
     1801 aaagttgttg gtgcagcaaa tgatagcgat gctaaaattg cagcaaaatc cattgccgag
     1861 tcacttttag ttaaaacggc aatttttggc aatgatccta attgggggag gattgcatgt
     1921 gccgctggat attctggtgc tgagttaagg gaggataaac tatcaatcta ttttgaggat
     1981 atatcgttat tcaaaaatgg cagtccagtc aactttgata aagacagatt gattgatata
     2041 ttaaaaagga aagaatatac aattatcata gactttggta ttgggaaatc attgtttgaa
     2101 tatttaacct ctgatttaac ttatgaatat gtaaagatta acgctgaata ttctacctga
     2161 taatatataa tccttgacgc aattgttata aactattaca attatgagca atttatcaat
     2221 actatttaat ttttttcaaa tgcggtatat ttcaaaaatt atagatttat ttcaactaaa
     2281 caacgttgtt aacaggttaa taatggcaga gtttaaaaga atagtatata gtattattat
     2341 gttttttatt tttatttctt tagtgaatgg gcaaactatt gataaacata agattattat
     2401 ttattatttt gaagatgaaa gttctttgca aaattacaga tattactcat atattatacc
     2461 tgataatctt gcgatagaac ttcaaaatca cgtggagtat gaagtaacgg ttttaccggt
     2521 aactttcaaa tatatttccg atgatatgcc tcaggaagaa aaagaaaata ctattacgat
     2581 cctttccaca agagctaaag aggttaatgc caggtttgtt atcattggtt cttataaagt
     2641 agaaaaaaaa agaattttta tacaaacaca aatttacaat actgtaaccg gtgaactaac
     2701 cgatgctgcc gaggtagaag aaactattgg tgcaatagta tatgaaatta ttgaaaagat
     2761 aacattaaaa attaatagcc agttgcagaa aacattagat gttgaaaaag aaaagattgc
     2821 actttccccc tataaagatt tttattcaac attccaaaat agttactttg gttattctta
     2881 tagttacgtc tttttcacgg gaagctggga aaacttatat aatgatccaa aaatgagtag
     2941 cgtctatatt ggtttccctc tatcgaaaat gtatgcttcg aactcggtgt ttataaaaaa
     3001 tatttatatt gttcctacat ggcaatttta ttcaactaac aatcatgatt taaaaaatat
     3061 gaatgattcc tatctatcaa tttataccgg atctatcgcg ttgcaatatc aatattcatt
     3121 atcccctttt atgattgttt ttgctagttt aggtgctggc gtttcgtatt cagatatgcg
     3181 ttttgttctt gcatcagaag gaggcccttt ctctcctgaa actaaaaccg atagtaccga
     3241 tccatatttt ggtgcaaacc tcggagcaat attttatata cataatatag gtattagtgg
     3301 tggacttgga tataaccgaa tattctataa aggagatgat ttagcatcat ttgttatgtc
     3361 ctttggcatc ttatatagat tataaaaaat atcacagtaa ctatcgcaca tatagttttt
     3421 caattttttt tatttaatca gttttttact acagttatag tgaaaagcaa tgaaagaatt
     3481 atcttattat tatgaaagga gatagtacca gtgaaatcta aaattgttta ttctcataat
     3541 gactactggt gtaagcacca gatttatgat gcaattgcgt atggttgtga tatggtagaa
     3601 gctgacgtaa tctatatgaa caataaaata atactgtcac actcatggcg ccctttttca
     3661 tttatgtgct atggcaattt agaagataac tatttaaagc ccctttcaca atactgcaag
     3721 gataatcctg ataaaaccat gtggatgtat gttgaattta aagactcaaa cgaacatatt
     3781 atcgaattat tgcataagtt atttaaacaa tatcaaacaa agaatttgcg atatactgta
     3841 tccgcactgg gtagtaaatg gtatcagaag aaaagatatg aaacagccat gcaattttac
     3901 aaaaaatata aagatgaatt ttcactttta tggaa
//
LOCUS       DAOOJH010000166         3904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_18014_length_3904_cov_10.177968, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000166 DAOOJH010000000
VERSION     DAOOJH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18014_length_3904_cov_10.177968"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /gene="mnmG"
                     /locus_tag="PLJ75_12415"
     CDS             <1..1449
                     /gene="mnmG"
                     /locus_tag="PLJ75_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPD06059.1"
                     /translation="AVILCTGTFLKGLIHIGNYNEKAGRLGDFSAEMLSDSLKALGFP
                     VKRLKTGTPPRVNGNTINFNKCEMQQPDPVPTPFSYRTKSIDRLQVPCYITHTTEKTH
                     AIIVNNLHLSPLYSGKIKGIGPRYCPSIEDKVVRFSSRNQHHLFLEPEGLHTNEYYIN
                     GFSSSLPEDVQLEMIKTIPGLEEVQVMRPAYAVEYDYVPPIELYHTLETKRVSGLYHA
                     GQINGTSGYEEAAAQGIVAAINAGNKISGKSPLVLQRSEAYIGVLIDDLVTKGADEPY
                     RMFTSRAEYRLLLRQDNADERIMKYGYEAGLIDKELYSDTLKKYKEINELIQKMSALS
                     IVVDEKIKIILSKKAIPIGEKASFNAGKLLKRPEITIDDILPIINEQDSSFTMTYDEA
                     KIVEMSIKYDGYIKKDLERIKKLKMMENKKIPATVDYSTIQGLKNEAREKLKTVRPET
                     IGQAMRISGVDPSDISIVLVHIESLNKNKTGA"
     gene            2411..3220
                     /gene="rsmG"
                     /locus_tag="PLJ75_12420"
     CDS             2411..3220
                     /gene="rsmG"
                     /locus_tag="PLJ75_12420"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPD06060.1"
                     /translation="MMNAGDNIDCNVINETLWEYASLIYDYNKITNITGFHTVEAIYK
                     ELIIKSITPLQNLNVPRGTSFVDIGTGAGIPGLALLLYFHGMVNGLLIDSNNKKIQFV
                     KEAVKKFDLHNYCTIIHGRAEDLAHDIHFREKFDFVVSRAFAHPYMAIEYGLPFVKVG
                     GFYYIYYQVDNFEQSIDEIKSTIGDRLYGEYRHGNGADKNNNTSLARDENYQMKNVLQ
                     HAEECGGHLASQEELMKRGLTGGLVVIKDKTMPLCFPRKNAVVKRIIHKLR"
     gene            3225..3797
                     /locus_tag="PLJ75_12425"
     CDS             3225..3797
                     /locus_tag="PLJ75_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06061.1"
                     /translation="MVNNKYKIFLLLMLNLISVGLLNYLAYAQVDNSRIAGMIIKDEK
                     LLVPGMGGGGVLLGSTEKDVMMTRAKSAIKTKSANHDFFKDILKVECQLTLPFNYLYK
                     YNEPECIIGFFNSTVEFIVVWDRRCILIEGVPISQGVMAVVYIYGNYGLACLREDNRS
                     LYIYAEKGIALIDEDNNDTIDGVIIFKTGS"
BASE COUNT         1335 a          599 c          792 g         1178 t
ORIGIN      
        1 gcagttatcc tgtgtaccgg gacattctta aaggggctga tccatatcgg caattacaat
       61 gaaaaagccg ggaggttggg ggatttttct gcagagatgc tatcggattc attaaaagca
      121 ttgggttttc ctgtaaaacg tcttaagacc gggacacctc ctcgtgtaaa cggtaatacc
      181 atcaatttta acaagtgcga aatgcagcag cctgatcctg taccaacacc tttttcatac
      241 cgtacaaaat caatagatag actacaggtt ccctgttata taacccatac aaccgaaaag
      301 acccatgcca ttatcgttaa taatttacat ctctctccac tctattcggg aaaaattaaa
      361 ggtattggac cgcgttattg tccttcaatt gaggataagg tagtacgatt ttcatcacgg
      421 aaccagcatc atctattttt agagcccgag ggtttacata ccaatgaata ttacattaat
      481 ggattttcaa gttcattgcc agaggatgta cagctggaga tgattaaaac aatacctgga
      541 ttagaagagg tacaggttat gcgccctgca tatgcagtag aatatgacta tgtacctccc
      601 attgaattat accatacttt ggaaacaaaa agggtatcag gattatatca tgcgggtcag
      661 ataaatggga cgtcaggata tgaagaagcc gcagcgcagg gaatagttgc tgcaataaat
      721 gctggcaaca aaatttcagg taaatccccc ctggtattac aacgttcaga ggcgtacatc
      781 ggtgttttga tagatgatct ggttaccaaa ggggcagatg aaccataccg gatgtttacc
      841 tcacgtgcag aatatcgatt gttattgcgg caggataatg ctgatgaacg tatcatgaaa
      901 tatggctatg aagctggact tatcgataaa gaattatatt ctgatacctt aaaaaaatac
      961 aaagagataa acgagcttat tcaaaagatg tctgcattgt cgatagttgt tgatgaaaaa
     1021 ataaagatta ttctaagcaa aaaagcaatt ccaatcggtg aaaaagcatc tttcaatgca
     1081 gggaagctgt taaaaaggcc tgagatcaca atcgatgata ttttgcctat tatcaatgaa
     1141 caggatagtt catttaccat gacatacgat gaggcaaaga ttgttgaaat gagtattaag
     1201 tacgatgggt atatcaaaaa agaccttgaa cgaataaaaa aattgaaaat gatggagaat
     1261 aaaaagatac ctgcaacagt tgattatagt accatacagg ggctaaaaaa tgaagctcga
     1321 gaaaagctga aaacagtaag gcctgaaaca atagggcagg ccatgagaat atctggagtg
     1381 gacccatctg atatttcaat tgtattggtt cacatagagt ctttgaataa aaataaaaca
     1441 ggcgcataag tatattgata acaatgtatt gtatagaaat attatatgtg aatacttcaa
     1501 caatcggtat gttccacgtg gaacattatg tcacattggc aatataatgg gtatagcatt
     1561 ataatatatt ttcataaaac attacggatg atataaggat gcccatccaa agccagcctg
     1621 aatgttatgg gaatatatat ccgggccatt gcagatgact acccacagga gagactccat
     1681 tttatgatgc caaaaatacg atgtcaaaag aggcaggcta tcgttatgaa accaatgtag
     1741 atttcagcct gtatttacgc ggtttcgcca aaattgataa cttttgaatg tatcaactta
     1801 tcaatcacca tgtcataaaa ggtcattaat gacctgccac attactttgc gatattaaca
     1861 aaatccctcc aagcaattgg gataaaaaat atatcttaaa aaatttccat agaccgaacc
     1921 agaggcatca actgataatt cctgaaatat ggtgtcgctc atctcaggta attcacatac
     1981 ggggcattac gtttgaagtc ttcaggaaaa tcaactttat tatcaactcc gccatgatgg
     2041 caatccttta tctctgcctt atactctgtc atatggatag atattttagg aatttaaaat
     2101 acttgttgcc tatcacttga agagggcttg ctgatgttgt ggctattctt cgtgagctga
     2161 actccaggct tctcttttct gccaactaac tgaagcataa gattgaaact gcatctttac
     2221 acggggcata tatcttttca gcatctcccc ggttcatgag gcatcatgta ctatatgcct
     2281 aaaacataat agtaaaaaaa tttctattga aaatttttag ctgagtaatt acaagtatta
     2341 aacagtacac tggcaactat ctctttcgtg cgatgaaata gttgtattaa aagtgcaaaa
     2401 gttggtatat atgatgaatg caggtgataa tattgattgt aatgttataa atgaaacatt
     2461 atgggaatat gccagtttaa tttatgacta taataaaata acaaatataa ctggatttca
     2521 tactgttgaa gctatttata aagaacttat tattaagagc ataacacctt tacaaaattt
     2581 aaatgttcca cgtggaacat ccttcgttga tataggaacc ggagcgggaa tccccgggtt
     2641 ggcattattg ctatacttcc atgggatggt caatggattg ttgatagatt ccaataataa
     2701 aaaaatacaa tttgtaaagg aagctgtgaa aaaatttgat ttgcacaatt attgtaccat
     2761 aattcatggg cgtgctgaag acctggctca tgatattcat ttccgtgaaa aatttgactt
     2821 tgttgtaagc agggcttttg cgcatccata tatggcaatt gaatacgggt taccatttgt
     2881 caaagttggt ggattttatt atatatatta tcaagtggat aattttgagc aaagtataga
     2941 tgaaataaag agtactattg gggatagatt gtacggtgaa tacaggcatg gcaatggtgc
     3001 agataaaaac aataatacga gtttggcaag agatgaaaac tatcagatga aaaatgtttt
     3061 gcaacatgcc gaagaatgtg ggggtcatct ggcatctcaa gaagaattga tgaagagggg
     3121 gttgacaggg ggattggttg ttataaaaga taaaacaatg ccattatgtt ttccaagaaa
     3181 gaatgcagtt gtgaaaagaa taattcataa attacggtag ctatatggtt aataataaat
     3241 ataagatatt tttattatta atgcttaatt taatcagtgt tggtttatta aattatcttg
     3301 cttatgctca ggttgataat agcaggattg cggggatgat aataaaagat gaaaaattat
     3361 tagttcctgg tatgggaggc gggggtgttc tgcttggttc aacagaaaaa gatgttatga
     3421 tgaccagggc aaagtctgct ataaagacta agagtgccaa tcatgatttt tttaaagata
     3481 ttctgaaagt ggaatgtcaa ttaaccttac ccttcaatta tctttataaa tacaatgagc
     3541 ctgaatgtat cattggattt ttcaatagta cggttgaatt tattgttgta tgggatcgac
     3601 gatgtatact catagaaggt gtcccgatat cacaaggtgt tatggcagtt gtatatatct
     3661 atggtaatta tgggttagca tgtttgcgag aagacaatag gtcattgtat atatatgctg
     3721 aaaaaggtat agctttaatt gatgaggaca ataatgatac tattgacggt gtcattatat
     3781 ttaaaactgg cagttaagtc aatagatgaa tttaatgaga acgataagat tatagtacgc
     3841 tatactacgc aatggaatat tttgaattta ttgtttcata gatataatgt gcgtggatat
     3901 cgtt
//
LOCUS       DAOOJH010000167         3882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_18146_length_3882_cov_9.423308, whole genome shotgun sequence.
ACCESSION   DAOOJH010000167 DAOOJH010000000
VERSION     DAOOJH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18146_length_3882_cov_9.423308"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            692..>1227
                     /locus_tag="PLJ75_12430"
     CDS             692..>1227
                     /locus_tag="PLJ75_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06062.1"
                     /translation="MSEQIRTIADKNQFAKIFSIFFLNNANVFLRTSSGDLKITFMGF
                     ADGTAAFKIPYVKNMSDTCIILTRKDNYLINGYFKFKEKDLDEMYVLYPMQFQIISNV
                     RREERVDVSSAGKSVLFFTNAISDYILQNSLALSLKKVDNVKEILRYDLDKRFPYVKI
                     YFLNEGLSDPRMKYFYNSP"
     assembly_gap    1228..1327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1365..1859
                     /locus_tag="PLJ75_12435"
     CDS             1365..1859
                     /locus_tag="PLJ75_12435"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HPD06063.1"
                     /translation="MDALHHIEPIIKKEIAIYNQLLAIEEQKKDAIIAFNGEQLFLFS
                     KEQEKYVHELENLEAARQNVLNNATKQGLQQGNISLKDFIMIASANNTENVVLLGYEL
                     KKKIEKFSRLYEGNKKLINDNMEFFNIILSSLRKATSYETGYGPEGLAMRSASGAVLM
                     NKTV"
     gene            1868..3778
                     /gene="flgK"
                     /locus_tag="PLJ75_12440"
     CDS             1868..3778
                     /gene="flgK"
                     /locus_tag="PLJ75_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HPD06064.1"
                     /translation="MNSTFMGIEIGKRGLLAHQQALHVTGHNISNAANEEYSRQRVVI
                     TASDPLYVPALNRAQQPGTIGQGAEVQSIERIRDEFIDDRIMVEKQAFGYWKTRNDFI
                     YQVEMVYNEPSDQSIRSRLDALWEGFQELSKYPEERSTREVVKEKAIALANEVKYVYG
                     KLYDLQKQANRQIFHTVSQINEYAQTIRDLNERIMKSEALGDNPNDLKDKRDALIEKL
                     SQLININVSRTDKDELIVYIGSENLVQGEVFRPLVAIEDPENNGLYRVLWKQTSTDVG
                     IQSGELAGLLSIRDSVLKQNINDVNAFAVNITDLINEVHRDGFGKNNQTNNNFFKAIP
                     VSDNVEGNFDLNNDGINDVTALFKISGNNKVDASAAIGINGTLSFVKNNAIDQEVKIN
                     YYATDTLLDVIQRINDAKIGVVGYINHNSQLAFKATIAEDTDKKNFIIRHLEDSGQLL
                     VGFAGILKESGPQGSFDYRRVDDIRKIIASREHITITPMFNPASYMDIDDAIKFDIDS
                     IAAAKGKDLGGTGDVNTTNGVGDGSNALAIAALKHKHAMIDANATFNDFYTSLIGRIG
                     SQGEEAKDRVANQETLLKNLANLRESVSGINLDEEMANMVQFQHGYNASARVIAMIDR
                     MLETIIKLGTGV"
BASE COUNT         1265 a          608 c          810 g         1099 t
ORIGIN      
        1 caattggcac tggattagga ttgtcaattg tttactcaac ggttaccaat gctggtgggt
       61 ttattcatgt tgagtcgcag gtaaatacag gaacaagctt aacaatttat ttgccatcac
      121 tggattgatg tcatgagcta tcgccaaaaa aagaaaaata gaaggccaac ttttctacag
      181 cttttcatgt caaccatggt accattcaat ttttaaaaat tttgtactgt cgattgatgt
      241 atgatgacta tacatggaca ttactatttg acataaaact atacgtatac tacatttata
      301 attgagatga gtttacgata gcggacttat cgacatgaat aaatgatgaa agagtgtcac
      361 ctttctgagg ctaaaccgat agtcattttg aacgaagtga agaatatcat cgttaaacga
      421 acggattctt tgattgctaa cactcactca gactgacaat gtggtttgtg taaatctgaa
      481 ccctggttca gaatctaaca ttgcctatct ggattacggg ttaagcccgg aatgatgttg
      541 aggaataaaa ctatttatga aaaagtatgt tgttatgaat aaatcattac aggtaaacgt
      601 ttgtatcatg taaaaaatgg ggaaattttt attgaaaaac agtccaatag tactaaaatt
      661 ggctgcgata aaaaattaat tggatcatac tatgtcagaa caaatacgta caatagctga
      721 taagaaccag tttgctaaga ttttttcaat ttttttcctt aataatgcta atgttttttt
      781 gcgaacctca agtggtgatt taaaaattac ctttatgggt tttgcagacg gtactgcagc
      841 atttaaaata ccatatgtta aaaacatgtc ggatacctgt attatactga cacggaaaga
      901 taactacctt atcaatggtt atttcaaatt caaagaaaaa gatctggatg aaatgtatgt
      961 tttatatccg atgcagtttc agataatttc aaatgtgcgc agggaagaaa gggttgacgt
     1021 atcgtctgcg gggaagtcgg ttttgttttt taccaacgct atttcagatt acatattgca
     1081 aaattcactg gctctttccc ttaaaaaggt ggataacgta aaagagattt tacggtatga
     1141 tcttgataaa cgtttcccgt atgtaaaaat atattttctc aatgaaggat tgtcagaccc
     1201 acgcatgaaa tatttttata atagtccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnntaa tagtagtatt agcatttttc caaggaggga atatatggat gccctgcacc
     1381 acatagaacc tatcatcaaa aaagagatag caatatataa tcagttattg gcaatagaag
     1441 aacaaaaaaa ggatgctatc atagcgttca atggggagca acttttttta ttttctaaag
     1501 aacaggaaaa atatgtacat gaattagaaa atttagaagc agcacgccag aacgtattaa
     1561 acaatgcaac aaaacaggga ttacaacaag gcaatatatc acttaaagat tttattatga
     1621 tagccagcgc taataatact gagaatgtag tattgctggg gtatgaatta aagaaaaaaa
     1681 tagaaaagtt ttcacggctt tatgagggca acaaaaagct cataaatgat aatatggaat
     1741 tttttaatat aatcctttca tcattgcgaa aagcaaccag ttatgagaca ggatacggtc
     1801 ctgaaggatt ggccatgcgc agtgctagcg gagcagtttt aatgaataaa acggtgtgag
     1861 gtataatatg aattcaacat tcatgggaat agagattggg aaacgaggat tacttgctca
     1921 tcagcaggcg ctacacgtaa caggccataa tatatccaat gctgccaatg aagaatactc
     1981 ccgtcagcgt gttgtcataa ctgcttcaga tccattatat gttcctgctc tgaatagagc
     2041 acagcagccc ggtactattg gtcagggtgc tgaggtgcaa agtatagagc gcattcgcga
     2101 tgaatttatt gatgaccgca tcatggtaga aaagcaggca tttgggtatt ggaagacacg
     2161 taatgatttt atctatcagg ttgagatggt gtacaatgag ccatccgatc agtccatacg
     2221 gagcaggctg gatgcactgt gggaaggttt tcaagaatta tcaaaatatc ctgaagagcg
     2281 ttcaacacgg gaagtggtaa aggaaaaagc aattgcactg gcaaatgaag taaaatatgt
     2341 atatggtaag ctatatgatc tgcagaagca ggcaaaccgg cagatttttc ataccgtttc
     2401 tcagattaat gaatacgcac agacgatacg tgatctgaat gagcggataa tgaaatcaga
     2461 agcactgggt gataatccca acgatttaaa agataaacgc gatgctttaa ttgaaaaact
     2521 ttcacagctt ataaatatta acgttagccg taccgataag gatgagctta ttgtatatat
     2581 tggatcggaa aatttagtgc agggcgaagt ttttagacct cttgttgcca ttgaagatcc
     2641 tgaaaataac ggtttgtaca gggtgttgtg gaaacaaaca tctactgatg tcggtattca
     2701 gagcggtgag ctggcaggat tgttatccat cagggatagt gtgttaaaac agaacatcaa
     2761 tgatgtcaat gcatttgctg tcaatataac cgaccttatc aatgaagtgc acagggatgg
     2821 ttttggaaaa aataatcaga ccaataacaa tttctttaaa gctataccgg taagtgataa
     2881 tgttgaaggc aattttgatc ttaataatga tggcattaac gatgtgaccg cgctttttaa
     2941 aatttccggc aataataagg tagatgcctc cgctgcaatt ggtattaacg ggacactttc
     3001 ctttgttaaa aataatgcta ttgatcagga agtcaagata aactactatg ctactgatac
     3061 attgcttgat gtgatacaac ggattaacga tgcaaagatt ggggtggtag ggtacatcaa
     3121 tcacaatagc cagctggctt ttaaggctac tatcgccgaa gatactgaca aaaagaattt
     3181 tattatccgt catctggaag attcaggaca gcttctggtg ggctttgccg gtattttaaa
     3241 ggaatcaggc ccgcaaggtt catttgatta ccggcgcgtg gatgatatcc gtaaaattat
     3301 agcttcacgg gaacatataa ccatcacacc aatgtttaat ccggcatcgt atatggatat
     3361 agatgatgca atcaaatttg atattgattc cattgctgct gctaaaggga aggatttagg
     3421 cggtaccggt gatgttaata ccaccaatgg tgttggtgat ggtagcaatg cattggcgat
     3481 agctgcatta aagcataaac atgcgatgat tgatgccaat gctacgttca atgattttta
     3541 tacctcgctg atagggcgta ttggttcgca gggtgaggaa gctaaggata gggtagcaaa
     3601 tcaggaaacg cttttgaaaa atcttgctaa tttacgtgag tcagtttccg gaattaatct
     3661 ggatgaagag atggctaaca tggttcaatt tcaacatgga tataatgcat cggcacgggt
     3721 cattgccatg attgacagga tgctggaaac cattattaaa ttaggtacag gggtataaac
     3781 catgcagcgc gttaccaatc agatgatcaa caacaatctt gtgtataacc tgcacaaaca
     3841 tgggcaggat atggatgcca tgcaaaatat gcttgcaacc gg
//
LOCUS       DAOOJH010000168         3850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_18336_length_3850_cov_10.166008, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000168 DAOOJH010000000
VERSION     DAOOJH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18336_length_3850_cov_10.166008"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..1457
                     /locus_tag="PLJ75_12445"
     CDS             432..1457
                     /locus_tag="PLJ75_12445"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06065.1"
                     /translation="MNYKDRKTNIPVLLLYNIDPCWELAEQQYASMGAEKLTNELQHE
                     GHPVIHIPVFNKSLCELLIKFNHNNYVVFNWCEELPGQNHSESKVAQILEEYHFTFTG
                     SSSNVIALSWDKALTKNILMYKNIPTPTGCIINDNNLDSWNKFPAIVKLAFEHCSVGI
                     NEDAVVTNKNELNQRYNYMKETYTQPVIVEDFLDGREFHVTVWGNGTIQTLPVAEFDF
                     SSFTDIKQHICTYSSKFVPGSSHYENISVTIPAKLNQSQLDTLNAIAIEAYKALGCRD
                     YARIDIREKNDMFYVIDVNPNPDFSPDTSSIYAGEIAGMSYGAMASYIVNLAALRHPV
                     FSKYIYN"
     gene            2304..2552
                     /locus_tag="PLJ75_12450"
     CDS             2304..2552
                     /locus_tag="PLJ75_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06066.1"
                     /translation="MTVSYDAKMVMQAFKERYGKDIKAGVTVSIDVLNDIPLSQRHLV
                     LDELINSLEFVEMIESKFPGKLFLTHRGYHFIQNIKEE"
     gene            2564..2848
                     /locus_tag="PLJ75_12455"
     CDS             2564..2848
                     /locus_tag="PLJ75_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06067.1"
                     /translation="MDALVSMLIELSRTFLQDVSDLPELSNLNKKLKEALDSAIADSS
                     DCNELNTVLRKYYSEIMMLYSKYPQSIFLDSLMHRIESLLQIANTIDGKL"
     gene            3026..3100
                     /locus_tag="PLJ75_12460"
     tRNA            3026..3100
                     /locus_tag="PLJ75_12460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3061..3063,aa:Pro,seq:ggg)
     gene            complement(3348..>3850)
                     /locus_tag="PLJ75_12465"
     CDS             complement(3348..>3850)
                     /locus_tag="PLJ75_12465"
                     /inference="COORDINATES: protein motif:HMM:NF021231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HPD06068.1"
                     /translation="VLIIVALCLGVAVAYALTVGQTVQNVELRDAENNPKMIPALGTK
                     LVSLFYNDADASDLNDPLADALKAKNFPKDKYEGFGIANLKDSKAPNFLIRKIIQGKI
                     KKYNTTILTDPDLILAKAWDLGDCNNTSVVMFIGKDMKVKYVYKGAVRGEEINKVVKM
                     VEDLIK"
BASE COUNT         1294 a          633 c          602 g         1321 t
ORIGIN      
        1 ttgcctgttc taaaagatag ttggtttgca tcattttact atattcaaac atactctggg
       61 tttatcctct tgacattatt tactattaga tgtatcggca tactataaaa ttcctttatt
      121 acattttttg tgacataata ttattattta tacaataact atttatgtaa tacaatgatt
      181 ttgaatatat tcaccatcta tgcactattg atataactac gttttttatt gaatacatta
      241 acatgtcagt cactcttttt tagagcagtt ttgaaactat ttttattgac attatgccaa
      301 ttaatttttt cattgtaaca tgcatatcaa ttggttagta aaaatttatt ttatagcgac
      361 tctatctttt cgatataata aatagagtag cagcaataat aaaatgaaac aacaacttac
      421 ataatgttat aatgaactac aaagatagaa aaacaaatat accggtatta ctgctctata
      481 atatagatcc ctgctgggaa ctggctgaac agcaatacgc tagtatgggt gctgaaaaac
      541 taacaaatga attacagcat gaaggtcatc ccgttatcca tataccagta tttaataaat
      601 cactatgcga actccttata aaatttaatc ataataatta tgttgtattt aactggtgtg
      661 aagaattgcc cggtcaaaat catagcgaaa gcaaagttgc acaaattctt gaggaatacc
      721 atttcacttt tacaggttct tcttcaaacg ttatagcttt aagctgggat aaggctttaa
      781 ctaaaaatat tcttatgtat aaaaacattc ctacacctac gggctgtatc ataaatgata
      841 ataatctgga ttcatggaat aaatttcctg caattgttaa acttgcattt gagcactgta
      901 gcgttggcat caatgaagat gctgtagtaa ccaataaaaa tgagttgaac cagcgttata
      961 actatatgaa ggaaacctat actcaacccg tgatagtaga ggattttctt gacggtcgtg
     1021 aattccatgt tacagtatgg ggtaacggca caatacaaac tcttcctgta gcagaatttg
     1081 actttagttc ttttacagat attaaacaac atatctgcac atatagttct aaatttgtac
     1141 cagggtcatc tcattatgaa aatataagcg tcacaatccc tgctaaactt aatcaatcgc
     1201 aacttgatac attaaatgct atagccatag aagcatataa agcattgggt tgcagagatt
     1261 atgcacgaat agatatccgt gaaaaaaatg atatgtttta tgtcatagat gtcaatccta
     1321 atcctgattt tagcccagat acttcctcaa tatatgcagg tgaaattgcc ggcatgtcgt
     1381 atggtgcaat ggcaagctat attgtaaatt tagcagcatt acgacatcct gtcttctcaa
     1441 agtatatata caattaataa caatattgtt tacggaatga attaccctaa catagtatta
     1501 tttatcaatg acattcaatt atttctcgac tatttgaaat aataaataaa attttaattt
     1561 acatataact atcactcatg ataaaatatt ttttatatgt aattctatta aatattatat
     1621 tttgcaacac aacaattaat ttcatgctcg cactgtcaaa tgaaaaacta ttccagtttg
     1681 tttattgaaa ataattacaa taaattaata tatatcaatt tgcttcatac tatgcatggc
     1741 actctggtat tttttcagaa gaattatatt gatttagcaa atatgctttt gttgacctac
     1801 aaaaaaatga gaaataattg ccagtcaaga attgagaaaa ttgcttgaag aaatatgtta
     1861 tggttatact aatatcacaa tatttttttc tgcatagagt attgtatgtt gcaattgcaa
     1921 tgaagaggct gtataattat ttttattgga ttttatcact acgtactatt tatcataatg
     1981 ctaatgaaag agtatatttc taccatttat agaggatgaa gcaaaaatgt cagtctgagc
     2041 tatagtcgaa gtcatcctga gcgatagcgc ctgtgctaag caaatccaaa gtaatgaatc
     2101 ttgctatgat gtgatattct gaacaaagtg aaaatcttgt agttttatga aagaatagat
     2161 tcttcaagac tgcgtccatc agaatgacaa tgtgtggaat gccatcctga aaatttcagg
     2221 ttttaaagtt aattattttt attccgtgtt aagcccagaa tggtattgat gggataattt
     2281 tatattcgag ggaggggtta tatatgacag ttagttatga tgcaaagatg gtaatgcagg
     2341 catttaaaga acgttacggc aaagatataa aagctggtgt tactgtttca attgatgtgt
     2401 taaatgatat ccctctatct cagcgtcatt tggtacttga tgaattaatc aattcattag
     2461 aatttgtcga gatgattgag tcaaaatttc ccggaaagct tttcctcacc catagaggct
     2521 accattttat acaaaacatt aaagaagaat aagtgctact gttatggacg ctttagtgtc
     2581 aatgctcatt gaactatccc gtactttttt acaggatgta tcagatttac cggagctatc
     2641 gaaccttaat aaaaagctta aggaagcgct tgattcagcg atagctgatt caagtgattg
     2701 caacgagctc aataccgttc ttcgcaagta ttacagtgaa atcatgatgt tgtattcaaa
     2761 atatccacaa agcatttttt tagattcgct tatgcatcgt attgaaagtt tattacagat
     2821 tgcaaacact attgatggca aattataata ccaaaggcta acttgttgaa acaaatttat
     2881 aatcagtagc aatatctatt gcctattaca tttatgtttt acgataccat tatatattaa
     2941 tgaatgctta atcaaaaaca gcgtaaatca aaaaaatgat tgaatttttt taacacatat
     3001 acggtatagt tataccatac atttacgggg cgtggcgcag tccggttagc gcactagact
     3061 gggggtctag gggccgctgg ttcaaatcca gtcgccccga attaagaaaa attattctga
     3121 tggagcatta gcaacctgct aatattcctt tttaacgatg agattctttg gctttgcctc
     3181 aggatgaaga taatgcttta taaccatcct cgagctgcat actatatttc ttttttcttg
     3241 tttatatagt taataaaaga gatagtgggg ttataaccag ttgacataaa taaaaaggga
     3301 tgtttctaca acatcccttt ttttgactta ctatactatt gtaaaaacta tttaattaaa
     3361 tcctctacca tcttaactac tttattaatt tcttcgcccc ttactgcacc tttgtacaca
     3421 tatttaacct tcatgtcttt accaataaac ataacaaccg aagtattgtt gcaatcaccc
     3481 agatcccatg cttttgctaa aattagatca ggatcggtta agatagtggt attatacttt
     3541 ttgatcttcc cctgtataat ttttctgatt aagaaattag gtgccttcga atctttaaga
     3601 ttagcaatgc caaacccttc atacttatct ttagggaagt tttttgcttt caaggcatct
     3661 gcaagcggat cattaagatc tgatgcatct gcatcattgt aaaatagcga taccaatttt
     3721 gttcctaaag ctggtatcat ctttgggtta ttttcagcat ctcgcaactc aacattctgt
     3781 accgtctgcc ctactgttaa agcataagca accgctacac ccagacaaag tgcaacaata
     3841 attaatacaa
//
LOCUS       DAOOJH010000169         3835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_18435_length_3835_cov_14.203175, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000169 DAOOJH010000000
VERSION     DAOOJH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18435_length_3835_cov_14.203175"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLJ75_12470"
     CDS             complement(<1..421)
                     /locus_tag="PLJ75_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06069.1"
                     /translation="MVDNPTGDDVYYEYGAPGAAYNAAGRVTKETAGKLIDQYRYGAL
                     GEVVKKTRTIEGKQYSITWKWDNFGRLRTIVYSNNVIIYYAYDTGGQVKQVFGYYNGT
                     RMDYVNNVYYDEFGSRTTIAYANGVVSQYSYDVLMHRL"
     gene            complement(482..676)
                     /locus_tag="PLJ75_12475"
     CDS             complement(482..676)
                     /locus_tag="PLJ75_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06070.1"
                     /translation="MLLQPGRDARRVGMQVHDRAACRRQHYTTSLSRQARKPVVHLPW
                     NGYICSNEGTPPYEVRFGGW"
     gene            complement(751..1374)
                     /locus_tag="PLJ75_12480"
     CDS             complement(751..1374)
                     /locus_tag="PLJ75_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06071.1"
                     /translation="MKKMMNKVILMIIISFVLPGCYDKDKSSDVKPKEERLINFPKKV
                     FGIEIGKLTANEVVQILNQRGIKYTIYRYEDYPKVPNIETTYFIGTGIRREAIQKVTY
                     DFNPFSGKNDKPDISIYIKSEYFELVLKDLIKLFGEPTRKDIDTIELKDKNGNRTKTV
                     KNLNYWWKSSKESESTLYFILLTKVLDKYSKCYILMRPKDIGCDHVH"
     gene            complement(1420..2967)
                     /locus_tag="PLJ75_12485"
     CDS             complement(1420..2967)
                     /locus_tag="PLJ75_12485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HPD06072.1"
                     /translation="MRAFNLWGVTASIRQPGKDARRIGMRVHDRAACRRQHYTTSLSR
                     QARKPVVRPPWNGYIHSNEKGVFLCILSFVAQQKKVRVKKYDANGNMTAVYDTAKQFN
                     RILYWDDDNRLTKTVDTTSGSSLTTTYAYDAKGMRIIKDGPYGKSIYIDTGYVESGAT
                     QTATAPIVSNHIFVGNTRVASVVKHTEEKQPATYFYASDHLGSSSVLTTQQGSYHERI
                     EYLPYGETWVEDKASSSGYSTPYKFTGKELDTETGLYYFGARYYDARISRWISADKYF
                     EEYLPTKPKENLNNIPGNGGVYRSINIDVYHYGGDNPLVIIDPDGNEDIIFVNNSNAT
                     YKKFESVALVYEDNTLDPVQLKLFKAWGAIKKAFGSSLNEKDAKMFFGNPDKEYDNFT
                     TLPNKPDNQGTAEEGILYNYKERDFKGLDAFVLWDEKLGTADDRKVPQAKAYNKGVNP
                     ATKNDYVQGSHMHTACGLKNGVKNPDNEGYDSGSRGCIVRYGGFSEFNNYLSDSQRNT
                     GRVIIFR"
     gene            complement(3442..>3835)
                     /locus_tag="PLJ75_12490"
     CDS             complement(3442..>3835)
                     /locus_tag="PLJ75_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06073.1"
                     /translation="LFSKGGEKFIVSGKDSNQFLTFKTLEEFMDSEYSKRYEGKYRVR
                     TSEKEDFEMQMAAIDEIGNEYSIFGRGEKTQDGKKEINCADFVYEVIEKGTKGRINID
                     RPEHRLGYTDPKEQFKNFKEKNKGIEIE"
BASE COUNT         1036 a          849 c          589 g         1361 t
ORIGIN      
        1 tgagccgatg catcagcaca tcgtagctgt actggctcac cacaccgttg gcgtatgcga
       61 tagttgttcg tgaaccaaat tcatcgtagt aaacattgtt tacgtaatcc atgcgtgtgc
      121 cgttgtagta gccaaacacc tgcttcacct gcccgccggt atcgtaggcg taatagatga
      181 taacattgtt gctgtagacg atggtgcgaa gcctcccaaa gttgtcccac ttccaggtga
      241 tgctgtattg cttgccctca atagtgcggg tcttcttcac cacctcgccc agtgcgccgt
      301 agcgatactg gtcaattaat ttccccgcgg tttccttcgt tacccgcccc gcagcgttgt
      361 aggcagcacc cggcgctcca tactcatagt atacatcgtc accggtaggg ttgtcaacca
      421 tttttaactg gtgaaagtga tagctccttt gtaaggggac atttatgcgc taaatgcccc
      481 cttaccaacc cccaaagcgc acctcatacg gcggtgtgcc ttcattgcta cagatatatc
      541 cgttccacgg caggtgcaca acgggcttcc tcgcctgcct tgacaacgat gtcgtgtaat
      601 gttgtctgcg gcaggctgcc ctatcatgca cctgcatccc aacacgccgg gcgtccctgc
      661 ccggctgtag tagcatacca gtagcctttc acggatggaa ggcgaactcg gttgcaggct
      721 gtaattcatc cttgtgcttt acttttacat ttaatggaca tgatcacatc caatgtcctt
      781 tggcctcatc aatatataac attttgaata tttatccaac acttttgtta ataatataaa
      841 atatagtgta ctttctgatt ctttgctgga tttccaccaa taatttaaat ttttaactgt
      901 ttttgttcta ttaccatttt tatcttttaa ttctattgta tctatatctt tacgggttgg
      961 ctcaccaaat aactttatca aatcttttaa tactaattca aaatattcac ttttaatata
     1021 gatagaaata tctggcttat catttttacc actgaatgga ttaaaatcat aggtgacttt
     1081 ttgtattgcc tctctccgta tgccggtacc tataaaatag gttgtttcaa tattgggaac
     1141 tttaggataa tcttcataac ggtatatagt atactttata cctctttgat taagaatttg
     1201 tactacttca tttgctgtta attttccaat ctctatccca aaaacctttt ttggaaaatt
     1261 aatcaatcgc tcttcttttg gttttacatc actgctttta tccttatcat aacatccagg
     1321 taatacaaaa ctaattatta tcatcaaaat tactttattc atcattttct tcataagaat
     1381 taatcctccc ttttacttat tcttctttga aatcgtatgt tatctaaaga ttataactct
     1441 tcctgtattc cgttgagaat cagataagta attattaaac tctgaaaacc ccccatatcg
     1501 tacaatacac cctcgcgaac cactgtcata accttcatta tcaggatttt taacgccatt
     1561 ttttaatcca caagctgtat gcatatgtga tccctgtaca taatcatttt tcgttgctgg
     1621 atttactcct ttgttataag cttttgcttg aggtactttc ctatcatccg cagttcctaa
     1681 tttttcatcc cataatacaa atgcatccaa acctttaaaa tctctctcct tataattata
     1741 cagtatccct tcttctgctg tcccttggtt atccggttta ttgggtagtg tagtaaaatt
     1801 atcatattcc ttatcaggat taccaaaaaa catcttagca tctttttcat tcagtgatga
     1861 cccaaatgct ttctttatag ctccccatgc cttaaataat tttaactgta ctggatccaa
     1921 agtattatct tcatatacta atgctactga ttcaaacttt ttatatgttg cgtttgaatt
     1981 atttacaaaa ataatatcct cattaccatc aggatcaata attactaagg gattatcacc
     2041 accatagtga tatacatcta tattgattga cctataaact cccccattac ctggtatgtt
     2101 atttagattt tcttttggtt tagtaggtaa atattcttca aaatatttat ccgcgcttat
     2161 ccatctactt atcctcgcgt cgtagtaccg tgcaccaaag tagtacaacc ctgtttccgt
     2221 atccaattct ttgccggtga acttatacgg tgtgctgtag cctgacgatg aagctttatc
     2281 ctccacccag gtttcgccgt agggcagata ctctatacgc tcgtggtagc ttccctgctg
     2341 ggtggtgagt acactgcttg aaccaagatg gtcgctggca taaaaatagg ttgctggttg
     2401 cttttcttcg gtatgtttta ccacgcttgc cacccgtgtg ttgcccacaa agatatgatt
     2461 gctcactatc ggtgctgttg ctgtttgtgt tgccccgctt tctacatagc cggtatctat
     2521 gtaaattgat ttcccatacg gtccatcttt gatgatacgc atgcctttgg catcgtacgc
     2581 gtacgtggtg gtgagactgc ttccgctggt ggtatctact gttttcgtta gccggttatc
     2641 gtcatcccag tacaggatgc ggttaaactg ttttgctgtg tcatataccg ctgtcatgtt
     2701 gccattggca tcgtattttt ttactcttac tttcttttgt tgggcgacaa aagaaagtat
     2761 gcaaagaaaa acgccttttt cattgctatg gatatatccg ttccacggcg ggcgcacaac
     2821 gggcttcctc gcctgccttg acaacgatgt cgtgtagtgt tgtctgcggc aggctgccct
     2881 atcatgcacc cgcatcccaa tgcgccgggc gtccttgccc ggctgccgta tgcttgcagt
     2941 aacaccccac agattaaagg cacgcactca aacatagaaa tcactctcta ttttatccta
     3001 acagacattc tcatttacca ttgaaactgt atgaattctt tttacggctt atatacattt
     3061 ttatttttac aagaaatatt cctgtaatgt aaaatataaa tgtgattatt atgcttataa
     3121 taaaatatat aaaacctaca gctaaacctt cataatcaac cccccagtca ggaaagtcat
     3181 atactatcat caaacaaata ttagtaaaat tagatacaaa taacaataaa aaataatttt
     3241 ttaacaattc tataatttta aaattttgta tgtttctaaa tattataatt gaatatgtta
     3301 tactatttaa cgaaataata ataaaataaa tcaatgatat acaatctata ctgcaagtgc
     3361 tattatcatc tacatgaaca taaagcattg caataataaa ataaattatc gaaaatatta
     3421 aaaggttgta atattttata ttcactctat ttctattcct ttatttttct ctttaaaatt
     3481 tttaaattgt tctttcggat cagtgtatcc taatctatgc tcaggtctat caatgttaat
     3541 cctccctttt gttccttttt ctataacttc atatacaaaa tcagcacaat taatttcttt
     3601 tttaccatct tgtgttttct cacctcttcc gaaaatagaa tattcattgc ctatttcatc
     3661 aattgctgcc atttgcattt caaaatcttc cttttccgat gtcctaactc tatatttacc
     3721 ttcatatcgt ttagaatact cgctatccat aaattcttct aatgttttaa atgtcaaaaa
     3781 ttgattacta tcttttcctg atactataaa tttttcaccg ccttttgaaa atagg
//
LOCUS       DAOOJH010000170         3795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_18696_length_3795_cov_11.249733, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000170 DAOOJH010000000
VERSION     DAOOJH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18696_length_3795_cov_11.249733"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="PLJ75_12495"
     CDS             <1..857
                     /locus_tag="PLJ75_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPD06074.1"
                     /translation="SSFNVFKASLDNGGIIKAIVVNDEGKISRAMIDEYINYVRTFKA
                     HGLPSVRYTGSNFETGIAKFLSDSEKQKLIDRLSLDSNSLIIFSCDKQKVVNDALGNL
                     RLKIAQQLTMIDDNKLNFLWVVDFPLLEYNAEEGRFYAMHHPFTAPKPECEAMLDSLT
                     PDNVAMIKAQAYDVVLNGVEIGGGSIRINRTTLQKKMFGILGISEEDAKIKFSFLLDA
                     LKYGAPPHGGIALGLDRILMILLQRNSIRDVIAFPKTQKGQCLMSEAPSPVSPEQLKE
                     LSIKVVEV"
     gene            857..1816
                     /locus_tag="PLJ75_12500"
     CDS             857..1816
                     /locus_tag="PLJ75_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPD06075.1"
                     /translation="MENTKFEIDDISIYKRICGYKDKNIKTIEKLLDVKIIPRGNTLI
                     VEALNDNAVKALQLLHCMGDYLYMRDSLYEFDDFDIRYLALSINQGIKVDADEINRLK
                     IFIPETGKTIVPKTINQAHYMSAIHKSPITISIGPAGTGKTYLAVAVGVKFLLTGRVE
                     RIILTRPAVEAGESLGYLPGDFIQKINPYLRPLYDALFDILPYERISKYMEKGIIEIA
                     PLAYMRGRTLNKAFVILDEAQNTTSSQMKMFLTRLGNNSKMVISGDITQIDLEKPKQS
                     GLLAAQKILHGIDEITFIEFLKEDICRHPIVEKIIAAYEKHTE"
     gene            1835..>3795
                     /locus_tag="PLJ75_12505"
     CDS             1835..>3795
                     /locus_tag="PLJ75_12505"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPD06076.1"
                     /translation="MLNSFMTFKRGIEFALKKKSIRLSFIYTIVVIVSATALLTINRT
                     GATYNYEAGDIAREDIRAPMNIEYPVALETEIEKSRIAESMPAVFDLNESIVEDKLKI
                     CNILCEAVIKVKQQMPDTNPFKQVLLLRTMLPDYLQYSDKVLIGLLQYDEQLLHRDVN
                     KVILQVYNNGILEKQYTNPLKLKSNFIVIRVIGNTSELKEKQVSIDALYDINKIKTLV
                     PVICKEVCNQPSNEVVYAISSVVMSLLQPNLFYNEEETKKRITDAIQSVKPVMRVIKK
                     GQSIAREGDTITEDVLKKIDIINKNSRFSGFSFVLGLLLTQLGFLIIFGYFLITYYTN
                     LVVDNKMPLIVFTLVMAFIIITFFMARWQGRMFEGLFFPLLLPIPLITMILAIMYNIH
                     IAMLIGMYIVFFVAIITKGDFTSLLLAFSSALSGVFVVGDVERRSDFLRGGFTLGLIN
                     SVIVVSVGLMNELSFAMILNNIGLAFVSGIINAIVVFGVFPIYENLFGLTTKFKLLEL
                     SDLNAPIFREMLIKAPGTYNHSIMVATLAESACKEIGANALLARVGGYYHDIGKIQDA
                     NFYIENKVTDPRAKKLSALEYCKLIISHVEKGVELARKNNLPEMVIDFIREHHGQTVM
                     TYFYHQALEESGASGDIIQKSDFQYPGPKP"
BASE COUNT         1191 a          625 c          792 g         1187 t
ORIGIN      
        1 gctcttcatt taatgtgttt aaggcttcgc ttgataacgg cggtattatt aaggcgatag
       61 ttgttaatga tgaaggtaaa atttcgcgtg ccatgattga tgagtacatt aactatgtac
      121 ggacatttaa ggcgcatggt ttacccagcg tacgctatac cggcagtaac tttgagacgg
      181 gaatagcaaa atttttaagt gatagcgaaa agcaaaagct tattgacagg ctttcgttag
      241 attcaaattc gcttattatt ttttcatgcg acaaacaaaa agttgtcaat gatgctctgg
      301 gaaatttaag gcttaaaata gcacagcagc tcactatgat tgacgacaat aaacttaact
      361 ttttatgggt tgttgatttt ccactgcttg agtataatgc tgaagaaggc agattttatg
      421 ctatgcatca cccttttact gctccaaagc ctgagtgcga agcaatgctg gactcgttaa
      481 caccggataa tgttgctatg atcaaagctc aggcgtatga tgttgtcctc aatggtgttg
      541 agattggtgg cggttccata cgaataaacc gtaccacgtt gcagaagaag atgtttggca
      601 tattggggat atcagaagag gacgctaaaa ttaaattttc atttttactt gatgcattga
      661 agtatggtgc cccaccccat ggcggtatag cactgggatt ggatagaatt ttaatgattt
      721 tgttacaacg aaattccata cgtgatgtca ttgcatttcc aaagactcag aaggggcagt
      781 gccttatgag cgaagctccg tcgccggtga gccccgagca gttaaaggag ctatccatta
      841 aggtggttga ggtataatgg aaaacacaaa gtttgaaatt gatgatatat caatctataa
      901 gcgtatatgt ggctataagg ataaaaatat aaaaacaatt gaaaaacttc tggatgtaaa
      961 gattatcccg cgtggcaata cattaattgt tgaagcctta aatgacaatg cagtaaaagc
     1021 gttgcagttg cttcattgca tgggtgatta tctgtatatg cgcgactcat tatatgaatt
     1081 tgatgatttt gacattcgct atctggctct ttctattaat cagggtataa aagttgatgc
     1141 tgatgagatc aacaggttaa aaatttttat acctgaaaca ggcaagacaa ttgtacccaa
     1201 aacaataaat caagctcatt atatgtcggc tatccataaa agcccaataa ccatttcaat
     1261 agggcctgcc ggtaccggta aaacctattt agcagtagcg gttggtgtta aatttttatt
     1321 aacaggaagg gtggaacgca tcatattaac acgtccggca gtagaagctg gtgaaagcct
     1381 tggatacctg cccggtgatt ttatccaaaa aattaatccg tatctgcgtc cactgtatga
     1441 tgctctcttt gatatccttc cttacgagag gatatcaaaa tatatggaaa aagggattat
     1501 tgagattgca ccactggcat atatgcgcgg aagaacgctg aataaggcat ttgttattct
     1561 tgatgaggct cagaatacca cctcctcgca gatgaagatg tttttaacac gattgggaaa
     1621 caattcaaag atggttatat cgggtgatat tacccagatt gatctggaaa aaccaaaaca
     1681 atcaggttta ttagctgcgc aaaaaatact gcatggtata gacgagataa cctttattga
     1741 atttttaaaa gaagatatct gcagacatcc aattgttgaa aagataattg cagcgtatga
     1801 aaaacatacc gaatagtatt atctggttat tattatgcta aattcattta tgacatttaa
     1861 acggggcata gaatttgctc ttaaaaagaa aagtataagg ctttcattta tatatactat
     1921 tgtggtcatt gtatccgcta ctgcgcttct taccatcaat agaacaggtg ctacctacaa
     1981 ttatgaggct ggtgatatag cacgtgagga tatccgtgca ccaatgaata ttgaataccc
     2041 ggttgcttta gaaacggaaa tagaaaaaag ccgtatagcg gaatccatgc ctgcggtatt
     2101 tgatctcaac gaatcaattg tagaggataa gcttaagata tgcaatatat tgtgtgaagc
     2161 tgtcattaag gttaagcagc agatgccgga caccaaccct tttaagcagg ttttattgct
     2221 cagaaccatg ctgcccgatt atctccagta ttcagataag gtattaattg ggctattgca
     2281 atatgatgag cagcttttac atagagatgt aaataaagtt attttacagg tttacaataa
     2341 tggaattctt gaaaagcagt atacaaatcc gcttaagctc aaatcaaatt ttatagtcat
     2401 acgggttatt ggcaatactt cagaattaaa ggaaaagcag gtcagtattg atgctctgta
     2461 tgacatcaac aaaattaaaa cattagtacc ggtaatatgt aaagaagtat gtaatcagcc
     2521 ctcaaatgag gtcgtctatg ctatcagttc ggttgttatg agtttgttac aacctaacct
     2581 tttttataat gaagaagaaa caaagaaaag aataaccgat gccattcaat cggtaaaacc
     2641 ggtgatgcgt gtaattaaga aaggacaatc aattgcgcgt gaaggggata caatcactga
     2701 agatgttttg aaaaaaattg atattatcaa taaaaattcc aggttttcag gtttcagctt
     2761 tgtactggga ttacttctta cccaattggg ttttctgatc atttttggct attttttaat
     2821 tacctattat accaatctgg ttgttgacaa taagatgcca ttgattgtgt ttacccttgt
     2881 catggcattt atcattatta ctttttttat ggcacgatgg cagggtagaa tgtttgaagg
     2941 gcttttcttt cctcttcttt tgccaatacc actgataaca atgatattgg ctataatgta
     3001 caatatccat attgccatgt taattgggat gtatattgta ttctttgtag caattatcac
     3061 aaaaggtgat tttacatcat tactgctggc tttcagttcg gcactttcag gagtgtttgt
     3121 tgtaggtgat gtagaacggc gatcagactt tttacgggga ggatttactt taggacttat
     3181 taactcggtg attgttgttt cagttggttt aatgaatgaa ttatcatttg ctatgattct
     3241 taataatata gggcttgcgt ttgtttcagg aattatcaat gcgatagttg tttttggagt
     3301 attcccaatc tatgaaaacc tttttggttt aacaacaaaa tttaaattgc tggaactatc
     3361 cgatcttaat gcacctattt tcagggagat gcttataaaa gctccgggaa catacaacca
     3421 ttcaatcatg gttgcaacgc ttgctgaatc cgcatgtaag gagattggag ccaatgcgtt
     3481 gcttgccagg gttggaggat attaccatga tataggtaaa attcaggatg caaactttta
     3541 tattgaaaat aaggttaccg atccgcgcgc taaaaagtta tctgcccttg agtattgtaa
     3601 attgatcatc tctcatgttg agaagggagt tgagctggcg cgcaaaaata atctacccga
     3661 gatggtaata gattttatcc gtgaacatca tggacaaaca gtaatgacat atttttacca
     3721 tcaggcactg gaggagagtg gtgccagtgg agatattatc cagaaaagtg attttcagta
     3781 tcccggtcct aaacc
//
LOCUS       DAOOJH010000171         3659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_19616_length_3659_cov_6.322974, whole genome shotgun sequence.
ACCESSION   DAOOJH010000171 DAOOJH010000000
VERSION     DAOOJH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19616_length_3659_cov_6.322974"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLJ75_12510"
     CDS             complement(<1..517)
                     /locus_tag="PLJ75_12510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD06077.1"
                     /translation="MAKKIIIVFVIVLVIAGAIVFGVAWNFSNQLISPQPYTCNNEHF
                     IYCGGASELGIPFEDVSFTNNENLTLKGWFFPTKTKTAIIMVHGITADRKEGLRWVKA
                     LHTAGYNLLLFDLRNHGESDRSISGMGLKEKEDVIAAVNFLVSQKGIKQVGVFGVSMG
                     ASTAIQAMAKDK"
     gene            609..2096
                     /locus_tag="PLJ75_12515"
     CDS             609..2096
                     /locus_tag="PLJ75_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPD06078.1"
                     /translation="MWHFAPTNVHWIDITLIVLYLAGITVFGFYYKSHIKTAKDYFLA
                     NRSLPWWVIGLSIIGTNIGSNDYIGAAGNAYSIGIAQANFEWIGAIPAMILSALIFIP
                     FFWRAGVYSIPEYLGLRYNNIVRFIAACVMSIFSVVIVGVFLWATAIMLQTYLGWPVW
                     FSIFITATVVGFYTISGGLGAVAITDTIQVFIMFFSAIIVAVIGIKTIGGFDSFIETL
                     KVNYPDHLNAFLPANHPAFPWHGVILGLGLVLSPAYWCANQAILQRTFGARSEWDGKA
                     SLIFAAMLKTFVPFLIVMPGLLALTLAGKSITHQDQALPWVIKNVLPAGISGLMFVAF
                     IAALQSSIDSTMNSTSVMIVRDIINVVRKEKLSDALQLKFGRIFTFCILIIGVIFAPI
                     TAYFQGIYVFIQYALSLFQGPIFALMIFGILDKRITATAGVVSLLTGLCFAALLGWFH
                     LNMLYIAFFSFCYSAIVLYIISTFTKKKSLSELKNVVYSSVVNIK"
     gene            2243..2446
                     /locus_tag="PLJ75_12520"
     CDS             2243..2446
                     /locus_tag="PLJ75_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06079.1"
                     /translation="MLQILYSLPLWTAKIAAMIIFSGILIIAWMLPYDFVMEGAPNKK
                     RWRDLRIWATLLTAVQIIIYYIF"
     gene            2903..>3659
                     /locus_tag="PLJ75_12525"
     CDS             2903..>3659
                     /locus_tag="PLJ75_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06080.1"
                     /translation="MIPIIAIPPLLMAAIAMYVAVSYGIMYSRRRNEPENLWFAIMCG
                     TIALYDIASAMLYISPSPFQAVLYQRFQFALLACFIIAITWFIASLVHIRTNIVYSIT
                     AIMVLFIISGFLLDSSYTLNPYNPYIKKFVFLGATITYNEADPGILYIAQYIAMLVAG
                     TYLMYKLFIWCRYGEVHIKILFASLLVFLLASINDVCVGKGMYHFIYILEYAYFIVII
                     SMAYILQSRFLTLHKEIEQVTYLFNQKLLEQQKQ"
BASE COUNT         1033 a          711 c          619 g         1296 t
ORIGIN      
        1 gcttatcttt tgccattgcc tgtattgcag ttgatgctcc cattgataca ccaaacacac
       61 caacctgttt tattcccttt tgactaacta aaaagttaac cgctgctata acatcctctt
      121 tttcctttaa ccccattccc gatatagatc tgtcactttc gccatggttt cgtaaatcaa
      181 atagtaaaag attatatccc gctgtatgca gtgcttttac ccatcgcaaa ccttcttttc
      241 tgtcagcggt gataccgtgc accataatga ttgcggtttt agtttttgtc gggaaaaacc
      301 atccttttaa tgtaaggttt tcattattgg taaatgacac atcctcaaat ggtatcccca
      361 gttcagatgc tccaccacaa taaataaagt gctcattgtt gcaggtatac ggttgtggac
      421 tgataagctg atttgaaaag ttccatgcta ccccaaaaac tatcgcccct gctataacca
      481 gtacaataac aaaaactata attatttttt tagccatata tcctccaaaa aatgtttgct
      541 attaaatctg tataatcata taatcgttaa cgatattatc aagcaaaaaa aatataaaag
      601 gggatactat gtggcatttt gcacctacca atgttcactg gattgatatt acactgatcg
      661 tactttattt agcaggcatt accgtttttg gattttatta caaatcacat ataaaaactg
      721 caaaagatta ttttttagca aacaggtcgc tcccatggtg ggttataggt ctttctatca
      781 ttggaacaaa tattggttca aatgattaca tcggcgctgc tggtaacgcc tacagcatag
      841 ggattgcaca ggcaaacttt gaatggatag gggctatccc tgccatgata ctatcggcac
      901 ttattttcat accgtttttc tggcgtgcag gagtatactc cattccagag tatctaggat
      961 tgcgctataa taatatcgtg cgttttattg cagcatgtgt catgagtatc ttttcagttg
     1021 taattgtagg tgtgttcttg tgggcaacag ctataatgct acaaacctat ttaggctggc
     1081 ctgtatggtt ttcaattttt ataaccgcaa ctgtagtagg attttacact atttcaggtg
     1141 gacttggcgc tgttgcaatt accgatacaa ttcaagtatt catcatgttt tttagtgcaa
     1201 ttattgttgc tgttattgga attaaaacta tcggtggatt tgattcattt attgaaacac
     1261 tgaaggttaa ctatcctgat catcttaatg ctttcctccc ggcaaaccat cctgcatttc
     1321 catggcatgg cgttatactt gggcttggat tggttttgtc accagcctat tggtgtgcca
     1381 accaggctat cttacaacga acatttggcg cacgctctga gtgggatgga aaagcttcac
     1441 tgatttttgc tgctatgctc aagacctttg tgccattttt aattgttatg cccgggttac
     1501 ttgctctaac ccttgctgga aaatctatca ctcatcagga ccaggcattg ccatgggtta
     1561 ttaaaaatgt tttacccgca ggcatttctg gactcatgtt tgttgcgttt atagcagcac
     1621 ttcaatcatc tattgactca acaatgaact caacgtctgt catgattgta cgtgatatca
     1681 tcaacgtagt acgcaaagaa aaattatcag acgcactgca acttaaattt ggaagaatat
     1741 ttactttctg catccttata ataggggtta ttttcgctcc cattacagca tactttcagg
     1801 gtatctatgt tttcatacaa tatgcgctgt ctctttttca aggtcccata tttgcattaa
     1861 tgatctttgg tattttagat aaacgtataa cagctactgc tggcgtggtt agtttactaa
     1921 caggactatg ttttgctgca ttgttagggt ggtttcatct caatatgttg tatattgctt
     1981 ttttttcttt ttgttattcg gcgatagttc tctatattat aagcacattt acaaaaaaga
     2041 agtcattatc tgaattaaaa aatgttgtat atagtagtgt tgtgaatatc aagtgaatag
     2101 tactgaacac atttatatag tttatgattg agcctttatt gctaatttct gttgcatgat
     2161 taataatcca attaccatgg tagatcagta agtattgatg tatatattgc attgcataat
     2221 catatcttaa gagggaattt atatgttaca gatactctat tcgttaccac tgtggacagc
     2281 aaagattgct gctatgatca tcttttcagg cattctcatt attgcatgga tgctccccta
     2341 tgattttgta atggagggtg cacccaacaa aaagcgctgg agggacctgc gtatctgggc
     2401 aaccttactt acagcagtac aaataatcat ctattatatc ttttaggtaa cacaattttc
     2461 attcttttca gattgccggg attttaccat ctcattggaa atagaattat tcaccccgcg
     2521 ctctcacctc cgtaagagca agaggatcat tgcttcctgt ttttataatt ttcatttcat
     2581 cctggcaaac ataagccggt caagggtaac tcatccgaac atagcaatat tactggatta
     2641 ttctgaagga gcagagcgac tgaagaatct atcctttcat atatcctttc atatataagt
     2701 attctttata taaaaaatat actaattttt atcctcttcc gtattttgta aacactacac
     2761 agccacttca ccttcacaat gcttctcaat tcataattca agatttttta tatacacctt
     2821 tttttatttt cttgcaaaaa agaataatca ctttttaata tttattgatg agaccctgat
     2881 gaatgaatgc tgagtattta ccatgatacc tataatagct ataccacctc ttcttatggc
     2941 tgctatcgcc atgtatgttg ccgtctcgta tggtattatg tattcacgca gaaggaatga
     3001 acccgaaaac ctgtggtttg ccataatgtg cggcactatt gctctttacg acattgcatc
     3061 tgctatgctc tatatatccc catcaccttt ccaggctgta ttgtaccagc gctttcagtt
     3121 tgcactactt gcctgtttca tcatagcaat aacgtggttc attgcttcac tggtgcatat
     3181 tcgtacaaac attgtttaca gcataaccgc cattatggta ttgttcatca tatcaggttt
     3241 tttgctggat agttcatata ctctcaatcc atacaatcct tacataaaaa aatttgtctt
     3301 tttaggtgca accatcacct acaacgaggc tgaccccggt attctttata tagcacaata
     3361 tattgcaatg cttgttgctg gaacatattt aatgtataaa ctttttatct ggtgcaggta
     3421 tggcgaagtg catatcaaaa ttttatttgc aagcttactg gtttttttgc ttgcaagtat
     3481 caatgatgtc tgtgttggca aaggcatgta tcattttatt tatattcttg aatatgcata
     3541 ttttatagtt attatatcca tggcatatat attacagtcg cgatttttaa cattacataa
     3601 agaaattgaa caggttacct atttatttaa tcaaaaactt ttagaacaac agaagcaac
//
LOCUS       DAOOJH010000172         3562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_20341_length_3562_cov_4.882236, whole genome shotgun sequence.
ACCESSION   DAOOJH010000172 DAOOJH010000000
VERSION     DAOOJH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20341_length_3562_cov_4.882236"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="PLJ75_12530"
     CDS             <1..1325
                     /locus_tag="PLJ75_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPD06081.1"
                     /translation="GFYILRNVCAGYTGKVYPVNPKYQELLGIPCYPDIESIPDRVEC
                     LIYFIPARFILDIIRRSAAKGIKAIIIESAGFAEAGKEGAKLQKDAVELAHSLGIRLW
                     GPNCMGYLDGHTRNVFSFVHSEKWKTMMHPGDVTLIVQSGMLSAGFLMSVLERDAIGI
                     SKVCSIGNKCDVNEIDLLEYFVQDDTTGVIGCYLESIVDGKKLLAIAQSTRKPIVMLK
                     SGRSEQGKKAALSHTASLAGNTMVVSGAFKQAGIVQVVDLYELIDIVKAFSYIKNYRP
                     EPDTGVAVMTFSGGAGIVTTDLLADYAIPIAQPQEATLQKVQKLYPSWMSASHPLDLW
                     PAIEQNGSEKVYTSVAKALMEDDGVDAIIIECFATQHSDPSFWGEIGTMVKQFKKPVL
                     AWAVGEKIIVEQYKKHAEASGIPFFGEIGRCVQIFKALRFHFNKRGRT"
     gene            complement(1374..2363)
                     /locus_tag="PLJ75_12535"
     CDS             complement(1374..2363)
                     /locus_tag="PLJ75_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06082.1"
                     /translation="MKLFTKVCLVLVLLFTSSTIFADWVYVPMSINANKGDVVLSTSP
                     GFIMDLLAILGCYWSHSGMAVDNGFNIRHNTMYVSDIPIEYNKIWFIQTTPKRMNPTR
                     LSNGLPGILTEDIDTAYNVTYNFAAAGGAILKPTAANEAAYRQYLNAAADVFNYLKAY
                     YRVNAYVNMYQLDYANYLITGRGNHCSGTCWYANYFSGKTMAVATIPANLVTQCANSL
                     YTSVKNMVRDEAGGFGSFIIDIEGLFGTGADEKIANQIVNTFGFDRATDTSDYWRSYI
                     GSVTATANAPDHLILSSYTNPSGHNVGVQTTSTSYYGQVEPLVITDGYYIEIP"
     gene            2834..3295
                     /locus_tag="PLJ75_12540"
     CDS             2834..3295
                     /locus_tag="PLJ75_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06083.1"
                     /translation="MGYAVKIYEAFKDDEIKAKLLSEFIELVEQAILNNQLATRQDVT
                     LSELKLTREIEQTRGEIKEVELKLTREIEQTRGEIKEVELKLTREIEQIRGEIKEVEL
                     KLTREIEQVRADVTEKINDVKISTIRWIVTLFITQTITILGIIAGLFQVMK"
     gene            3370..>3562
                     /locus_tag="PLJ75_12545"
     CDS             3370..>3562
                     /locus_tag="PLJ75_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06084.1"
                     /translation="MKKKVMVYYSYIVTIPIFLLSCATMNTADTRLVDQLKAENRVCS
                     QNLNLFMRENDVLKAENAKY"
BASE COUNT         1134 a          594 c          806 g         1028 t
ORIGIN      
        1 atggttttta tattctgcgc aatgtttgtg ctggttatac aggcaaagtg tatccagtaa
       61 atcctaaata tcaggagttg ttgggtatcc cgtgttatcc tgatattgag tcaattcctg
      121 acagggttga atgcttaatc tattttatac cagcacgctt cattctggat attataagaa
      181 gaagtgcagc aaaggggatt aaggcaatta tcattgaatc agccggcttt gccgaggcag
      241 gcaaagaagg agcaaaattg cagaaagatg cggtagagct ggcgcacagc cttggcatac
      301 ggttgtgggg tcccaattgc atgggatatt tagatggcca tacgcggaat gttttttcat
      361 ttgtccattc tgaaaagtgg aaaacaatga tgcatcctgg tgatgtgacg cttatagtac
      421 aaagtggcat gctttcggca gggtttttaa tgtcggtgct ggaacgtgat gcgataggta
      481 ttagcaaggt atgttctatc ggaaataaat gtgatgtcaa tgaaattgat ctgttagagt
      541 attttgttca ggatgataca acaggagtga ttggttgcta tctggaatca attgttgatg
      601 gaaaaaaact gttagcaata gctcaaagca cccgcaaacc aatcgttatg cttaaatcag
      661 ggagaagtga acagggaaaa aaggctgccc tgagccatac agccagtctg gcgggcaata
      721 ctatggttgt gtcaggagct tttaaacagg ctggaatagt gcaggttgtt gatctgtacg
      781 aattaattga tattgtcaaa gctttttcat atatcaagaa ctatcgcccg gagccagata
      841 caggtgttgc ggtgatgaca ttttcaggcg gcgcaggtat tgtaacaact gatctgctgg
      901 ctgattatgc tatcccgata gcacagcctc aagaagcgac attgcaaaaa gttcaaaaat
      961 tatatccttc atggatgagc gcttcacacc ctcttgatct atggcctgct attgaacaga
     1021 atggctccga aaaagtttat acatcggtag caaaagccct tatggaagat gacggggttg
     1081 atgctatcat cattgaatgt tttgcaaccc agcattctga tccatcattt tggggtgaga
     1141 taggtactat ggtaaagcag ttcaaaaaac cagtgcttgc atgggcagtt ggtgagaaga
     1201 taatagttga acaatataaa aaacatgccg aagccagcgg catacctttt tttggtgaga
     1261 ttggacggtg cgtacagata ttcaaggcgt tgaggtttca tttcaataag cgagggagga
     1321 cataaaaaaa tcccgggatt aacccggggt tttttattat tgatgtgtgg catttaaggg
     1381 atttcgatat aataaccatc agtaatgaca agaggttcaa cctgtccgta gtatgatgtt
     1441 gatgttgttt gcacaccgac attgtgccct gacggatttg tataggatga aagtatcaga
     1501 tgatcgggag catttgctgt tgctgttacc gaacctatat agcttctcca gtaatcggat
     1561 gtatcggtag ccctatcaaa cccgaaggtg tttacaatct ggttggctat tttttcatca
     1621 gcgcctgtac cgaataagcc ttcaatatcg atgatgaaag aaccaaatcc tccagcctca
     1681 tcacgaacca tattctttac tgaggtatac agcgagttgg cacactgtgt caccagattg
     1741 gctggtatgg ttgcaacggc catagttttt cctgaaaaat agttggcata ccagcaggta
     1801 cccgagcagt ggttgccacg tccggtaatg agatagtttg cataatcaag ctgatacatg
     1861 ttaacataag cattaacgcg ataatacgct ttaaggtaat taaaaacatc tgctgcggcg
     1921 ttaagatatt gacggtaggc tgcctcattt gcggctgttg gctttagtat agctccgccc
     1981 gcagcagcaa aattgtacgt tacattataa gcagtatcaa tatcttcggt taagatgcca
     2041 ggcagtccat tactgagccg tgttggattc atccttttag gtgttgtctg gatgaaccat
     2101 atcttgttgt attctattgg aatgtcgcta acatacatgg tgttatggcg gatattaaat
     2161 ccgttatcaa cagccatgcc actgtggctc cagtagcagc ccagtatagc cagtaaatcc
     2221 ataataaaac cagggcttgt tgaaagaacc acatcacctt tgttggcatt aatgctcatg
     2281 ggtacatata cccaatcagc aaagatggtg gaactggtaa atagcaatac cagcacaaga
     2341 catacttttg taaaaagttt catataacct ccaaacttat aattaaatta ataaatgatc
     2401 gggatatacg tgtcgatggc aaaatgagtg attgctcact cattactatt cattggatac
     2461 acaataataa cattatcgga aaaagtcaag tattaaatac catatggtcg gtatttaata
     2521 atgtttttat agttattatt ttgattatat aataatgtat gcacttcttt ttttatagca
     2581 ggtgattatt gagttaaaaa cctgctataa tgctaaattt ttattgtttt gtgcgacagt
     2641 acttacatca ataaaattgt tatatcaact ccgtatgggt gaaataattt tagaagattg
     2701 tataatcaca aggtgataaa tccaatcggg tgcgatatag tataggtgat agtatatgaa
     2761 taaaaataat ttgacatgcc gatggcaagc aatatagtat aacacatatg caaatagagg
     2821 aggtactgtt ccaatgggat atgcagtaaa gatatatgag gcattcaaag atgatgaaat
     2881 aaaggcgaag ctattgtctg aatttattga attggtagaa caggctatat taaataatca
     2941 gttagctaca cggcaggatg taacgctgtc agaattaaag ctaaccagag agatagagca
     3001 gacgcgcggt gagataaaag aggttgaatt aaagctaacc agagagatag agcagacgcg
     3061 cggtgagata aaagaggttg agttaaagct aaccagagag atagagcaga tacgcggtga
     3121 gataaaagag gttgaattaa agctaaccag agaaatagag caggtgcggg ccgatgtaac
     3181 agaaaaaatc aatgatgtaa agataagcac catacggtgg atagtaacat tatttataac
     3241 acagacaatt acaatactgg gtatcatagc gggattattt caagtaatga aatgatactt
     3301 tcaaagccac acacttcggc aggttcagaa tgacaatttt aaataattct aaatgtggag
     3361 agatacataa tgaaaaaaaa agttatggtg tattatagtt acattgttac tatccccatt
     3421 tttttactat catgtgcaac catgaatact gctgatacca ggcttgttga ccagcttaaa
     3481 gctgaaaaca gggtatgttc gcaaaatctg aatcttttta tgcgtgaaaa tgatgtgctt
     3541 aaagctgaaa atgctaaata ta
//
LOCUS       DAOOJH010000173         3476 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_20944_length_3476_cov_5.344636, whole genome shotgun sequence.
ACCESSION   DAOOJH010000173 DAOOJH010000000
VERSION     DAOOJH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20944_length_3476_cov_5.344636"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..568)
                     /locus_tag="PLJ75_12550"
     CDS             complement(389..568)
                     /locus_tag="PLJ75_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06085.1"
                     /translation="MFYRLICHNVGNVVDNSIKEILQSANKYYEGKYILCFAKYGNID
                     SATLPLEITIARQEK"
     gene            complement(595..970)
                     /gene="rnpB"
                     /locus_tag="PLJ75_12555"
     ncRNA           complement(595..970)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLJ75_12555"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(971..1561)
                     /locus_tag="PLJ75_12560"
     CDS             complement(971..1561)
                     /locus_tag="PLJ75_12560"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPD06086.1"
                     /translation="MGTAHTIRIIGGSFKGKVIPYEKKLQGAEASSQRLKKALFSIAG
                     EDLSGKNFLDLFSGTGQIGLEALSRGAKLVIFNDADRKRYQFVKDIVVQWQLLQRARN
                     YNCDAFRYLKLCSEEGLVFDIIFCDPPFEKIAELPQIYYLLLQDLSNSSCVAVNTYIF
                     LQHYHTNSMPDSIGRLYKRDTRKYGTNALSIYRVSE"
     gene            complement(1565..1771)
                     /locus_tag="PLJ75_12565"
     CDS             complement(1565..1771)
                     /locus_tag="PLJ75_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPD06087.1"
                     /translation="MKITEDMKVKEVIAAHVQAAKVFKKYNLGCIGCRGADEDTIKMV
                     ATNYGLDVQQFVKDINEAIKEAKN"
     gene            complement(2639..>3476)
                     /locus_tag="PLJ75_12570"
     CDS             complement(2639..>3476)
                     /locus_tag="PLJ75_12570"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HPD06088.1"
                     /translation="GRYIVSGSWDKTIKVWDATTYKEVATLTGHYSTVYSVAYSPDGK
                     YIVSGSEDKTIKVWDARTYKEVAKLIWHDFGVESVAYSPDGKYIVSGSNDYTIKVWDA
                     QTYTGVATLRGHFISVTSVAYSPDGRYIVSGSEDKTVKIWDAITYKNVATLRGHNDDV
                     ESVAYSPDGRYIVSGSRDNTIKVWDATTYTAVATLRGHSDWVYSVAYSPDGRYIVSGS
                     RDRTIKVWDATTYKEVATLRGHGGEVNSVAYSPDGKYIVSGSFDRTIKLWDMQVILPK
                     VF"
BASE COUNT         1001 a          834 c          507 g         1134 t
ORIGIN      
        1 gataactgtt ctaagctcca tgctattcac cttctaaaat ttttttattg tactggtgaa
       61 gtattgtaat aggataacaa tatcaagtct ataaaaataa tatagctttc aatatactgt
      121 gaatagacaa tactggtaca atataaaaaa acttttaaaa actggatatg caaccacttt
      181 attgtcacaa attctcagaa acagattttt aaaacattga cacttattaa ccaatgtata
      241 cggcaaacca ccattattgt aatcgttatt ttacaatcat gtgtataaca actgttgatt
      301 ttttagacgt tcaatattgg aatatcatga ttccacattg aacaccgaca gcaggaacaa
      361 taaacattac caaatcgcct ctattaagtc acttttcctg ccgggcgata gttatttcta
      421 atggtaatgt tgccgaatca atgttcccgt acttagcaaa acacagtata tatttacctt
      481 cataatattt attggcactc tgcaaaattt cttttatgga attatcaact acattcccca
      541 cattatgaca tatcaatctg tagaacacag cttatctcta ttcaattata agcaggtaag
      601 aggggcacat aagccgaatt ctgtttatgc agccattaat cttgtgggca tgttaccata
      661 cccatctttg cggcctaccc gcaacctcaa gcgggcaact ttgatagcgg ttgcctattt
      721 ggccttgcac cggatggggt ttgcactgcc atcggtattg ctaccgatgc ggtgagctct
      781 tacctcgcct tttcaccctt acccttcaac tttactcagg gcaagcccca ggtaaaatca
      841 aaaggcggta tgttttctgt tgcactttcc atcgcctcac ggcgcccggg tgttacccgg
      901 catcctgccc tgcggtgttc ggactttcct caccgggtgt gtttcccggc gcggctgcct
      961 gtgcccctct ttattcacta accctgtaaa tactcagcgc attggtacca tatttacggg
     1021 tatcccgctt ataaagcctt ccaatactat caggcatact attggtatga taatgctgca
     1081 aaaaaatata cgtatttaca gccacgcaac tgctgtttga taaatcctgt aataaaagat
     1141 aatatatttg gggtaattca gcaatctttt caaatggagg atcgcaaaaa ataatgtcaa
     1201 ataccaaacc ctcttcactg cacagcttta aataccgaaa ggcatcacag ttatagttac
     1261 gtgccctttg cagtaattgc cattgcacaa caatatcttt tacaaactgg taacgcttcc
     1321 tatcagcatc attaaatata accagctttg cacccctgct taatgcttca agcccaatct
     1381 gccctgtccc ggaaaacaga tctaaaaaat ttttaccgct taaatcctca ccggcaatag
     1441 aaaataatgc cttttttaat cgctgactgg atgcttcagc cccctgcagc tttttttcat
     1501 aggggataac cttaccttta aagctgccac ctataatccg tatcgtgtgc gcagtaccca
     1561 tgaactaatt ctttgcctct tttattgcct cgtttatatc tttaacaaac tgttgtacat
     1621 caagtccata attagttgcc accattttta tggtatcctc atctgctccc ctgcaaccaa
     1681 tgcagccaag attatatttt ttaaaaacct ttgctgcctg cacatgggcg gctataactt
     1741 cttttacctt catatcctca gtaatcttca tatatccccc caaaaattaa attataccac
     1801 atcattgtta tgaaccggca gcatttttgt cattcctgta aaactatctt caaagaatct
     1861 ccttgtacaa cactgaaaca ttttgctatc attgagctgg acattgctgc tgcacgtatc
     1921 acaacattag ccgtcgcata ccgccatcaa aacggtacag gtacattgtc atcttctttt
     1981 attattataa acctatttca catttcttca aaatgttcat cgggtaataa tgcaatactc
     2041 accctgttgt accctgtcac tatatcatga taaaaaattt tttgctttca aagaccctcg
     2101 tgttttactg ctttgtttta tgctgcaaat atagtaaact ggcttattta atcaagcatt
     2161 attgaggatt gcgtttttca tattttgcgt ttattgcgtc aatatccttt ttttatacat
     2221 atctgaccat ctctgccgtt tttttcgtct ataaaacaat acatcaatca gtttttcccg
     2281 ttcccctttg tccttcttgt tgtatatcca tgacatatct tttcatgtaa gcactatatg
     2341 gctatacttt tcccacaatc cctgtctacc atatacgcaa tttttccctg agaatgatga
     2401 ttttttatat ggctgataat aaatattttt agcagtataa aataattaat atttcaggta
     2461 aacctggcat tgccatacac tacccccctt atagagatat tgtgtacatc catgatacta
     2521 tataaccata aaaaacatga agcattaatc atacatttaa tataactatc accatacata
     2581 tatacttcaa tgcacggctg ccgatatgat gataaatccc gaagcaaata ttatctattt
     2641 aaaaaacttt tggtaaaatt acctgcatat cccataactt gatggttcta tcaaaactcc
     2701 ctgaaacaat atacttccca tcggggctat acgctacgga attaacttca ccaccatgcc
     2761 ctcgtagtgt tgccacctcc ttataggttg ttgcatccca taccttgatg gttctatccc
     2821 tacttcctga aacaatatac cgcccatcag gactatacgc tacagaataa acccaatcac
     2881 tatgccctcg tagtgttgcc acagctgtat aggttgttgc atcccatacc tttatagtgt
     2941 tatccctact tcctgaaaca atataccgcc catcggggct atacgctacg gattcaacat
     3001 catcattatg ccctcgtagt gttgccacat tcttataggt tatggcatcc catatcttga
     3061 cggttttatc ttcacttcct gaaacaatat accgcccatc ggggctatac gctacagatg
     3121 taacactaat aaaatgccct ctaagcgttg ccacccctgt ataggtttgt gcatcccata
     3181 cctttatagt gtaatcatta cttcctgaaa caatatattt tccatcgggg ctatacgcta
     3241 cagattcaac accaaaatca tgccatataa gttttgcaac ctccttatag gttctggcat
     3301 cccatacctt aatggtttta tcttcacttc ctgaaacaat atactttcca tcagggctat
     3361 acgctacgga gtaaacagta ctataatgcc ctgtaagtgt tgccacctcc ttataggttg
     3421 ttgcatccca taccttaatg gttttatccc aactccctga tacaatatac cgccca
//
LOCUS       DAOOJH010000174         3457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21080_length_3457_cov_7.007349, whole genome shotgun sequence.
ACCESSION   DAOOJH010000174 DAOOJH010000000
VERSION     DAOOJH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21080_length_3457_cov_7.007349"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PLJ75_12575"
     CDS             <1..659
                     /locus_tag="PLJ75_12575"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD06089.1"
                     /translation="EYTPALRQYITNIISFKEKLYETIALDYEKQKQWDKAISLYNAM
                     LAVNPNNFTANYRIGIINATIQNLENAFKYLNNAMRMDPNNPDVLYQLGILLVSQGKF
                     NEALQYLTQAHKMNMASASLFFNIGLCYESLKNLEEARRYYEKAKLIDPNDATIQSSL
                     ERVSEQKVQQVDQNTDTRTNQIEEEKGESFPLPINQSAYDVRLKENELKDQNTSRVQQ
                     "
     gene            722..2413
                     /locus_tag="PLJ75_12580"
     CDS             722..2413
                     /locus_tag="PLJ75_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPD06090.1"
                     /translation="MNGFSIGITLFGGLGLFLFGMKIMSESLQLATGERLKSILWKVS
                     NSRIKGVFTGFFITAIIQSSSATTVMLVSFVSAGLLTLQQSIGIIFGANIGTTVTGWL
                     VALIGFKIKITAIALPCIGLGFFIRFISNEKIKNYGEVLLGFGVLFFGLDIMSSAVKD
                     LRNSEAILNIMAHYSADDIPSTLIVIAIGTIVTMIVQSSSATVAMTMTLAVNGLIDFP
                     TACAFILGENIGTTITANIAALSASSEAKQAARAHFLFNAIGVIWLMLIFHMFFIPFV
                     DWLVPGNPYSDILAVRSKVIADHMAAFHTCFNVINTIVFLPFANILTNLAIKLAPQSN
                     DVPDEFHLKYITSILVSTPTININQARLEIKRMSNIVLEMFDMVMDVFHHPEEKLGTQ
                     VEQILKLENLTDHLEKEISSFLVSVLQNNISYEQSEEISSLLHTVNELERIGDNCESL
                     LKLLRRKYEQNIQFSDEAVKGIDEIASKVREFMVLINQNITVRNKDIMADSKFLEDRI
                     DELRNELRKGHVNRLNEGVCNVDAGLMFIDMLSKFEKIGDHAFNVAESISGVRIF"
     gene            complement(2393..2911)
                     /locus_tag="PLJ75_12585"
     CDS             complement(2393..2911)
                     /locus_tag="PLJ75_12585"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPD06091.1"
                     /translation="MKRLFIGMLFIGGLALMAFTYHNDILLRQEPWIELSNTQEKYKG
                     VTWKITAYCPGSCCNKRITGTKVVDYTNSAAIGGLKLTTLLDNTINVVAVDRSIIPLG
                     SIVKYNNNYYVALDTGSAIKGFTLDILMPHHKQTEKFGIQYSDNIEVYIPHNPAQIIK
                     KIKKIALEYSNP"
     gene            3142..>3457
                     /locus_tag="PLJ75_12590"
     CDS             3142..>3457
                     /locus_tag="PLJ75_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06092.1"
                     /translation="MIGNIATLVMCLAMILFFRRLDRSNLRITKLKRYTSKVLDDFKK
                     LTQDEERKFSDATIELDIMLKKAQALSTHLSSSISGIEEKLKGLDIEKTNLKKVEEDL
                     KVI"
BASE COUNT         1157 a          568 c          616 g         1116 t
ORIGIN      
        1 ctgaatatac ccctgccctg cggcagtata taacaaacat cattagcttt aaagagaaat
       61 tgtatgaaac cattgcactg gattatgaaa aacaaaaaca atgggacaaa gctatttccc
      121 tctacaatgc aatgttagcg gttaatccca ataactttac tgctaattat cgcattggta
      181 ttatcaatgc aaccattcaa aatttagaaa atgcatttaa gtatttaaat aatgcaatgc
      241 ggatggaccc caataatcct gatgtactgt atcagttagg gatacttctt gtatctcagg
      301 gaaaatttaa tgaagcatta cagtatctta cacaggcaca taaaatgaat atggcttcgg
      361 catcattgtt ttttaacatt ggtttatgct atgaatcact aaaaaattta gaagaagcca
      421 gacgatacta tgaaaaagca aaacttattg atcccaacga tgctacaata caatcaagtc
      481 tggaaagagt ttcagaacaa aaagtacagc aagtggatca aaacactgat acaaggacca
      541 accagataga agaggaaaaa ggtgaaagct ttccattgcc aataaatcaa tctgcctatg
      601 atgttcgtct caaagaaaac gaactgaaag atcagaacac atccagggtt caacaataac
      661 gttatagcat tcttttacta atattttttt actaatactt tttattatta tgaggttttg
      721 catgaatgga ttttcaatag gtattacctt atttggtgga ttaggattgt ttttatttgg
      781 catgaagata atgagcgaat ccctgcaatt agctacaggt gaacgtttaa aaagcatttt
      841 atggaaagtt agtaatagca gaattaaagg agtatttaca ggatttttta ttactgccat
      901 cattcaatct tcaagtgcca ctactgttat gctggtaagc tttgttagcg ccgggctttt
      961 aactctgcaa caatcaattg ggatcatttt tggtgcaaac ataggcacta cagtaaccgg
     1021 atggcttgtt gcattgatag gttttaaaat aaaaattaca gcaatagctc tcccatgcat
     1081 tgggttaggc ttttttatac gtttcatatc aaatgaaaag attaaaaact atggtgaggt
     1141 tcttttagga tttggtgttt tgttttttgg gcttgatatt atgagcagcg cagttaagga
     1201 cttgcgaaac tcagaggcaa ttcttaacat tatggcccat tatagtgcgg atgatatacc
     1261 atctactcta attgtaattg caattggaac aattgtaacc atgatagtcc aatcatccag
     1321 tgcaacagtt gccatgacaa tgacgttagc agttaatgga ctcattgatt tcccaacagc
     1381 atgtgcattc attttaggtg agaatatagg tacaactata accgccaata ttgctgcttt
     1441 gagtgcatca tccgaagcaa aacaggcagc gcgtgcacat ttccttttta atgccatagg
     1501 ggtgatatgg ttgatgctta tattccatat gttttttata ccgtttgttg attggctggt
     1561 acccggcaat ccctattctg acattttagc agtacgatca aaagttatag cagatcatat
     1621 ggccgcattc catacatgtt ttaatgtaat taatactatt gtttttttgc catttgcaaa
     1681 tattcttaca aatcttgcca taaaattagc cccacaatca aacgatgtgc cggatgaatt
     1741 ccacctaaaa tatattacct ccattctggt atctactcct acaataaata tcaatcaggc
     1801 acgtttagag attaaacgca tgagcaatat tgtacttgaa atgtttgata tggttatgga
     1861 tgtcttccat catccagaag aaaaattggg cacccaggtt gagcaaattt taaaattaga
     1921 aaatcttacc gatcatcttg aaaaagagat ttcaagtttt ctagtaagcg ttttacaaaa
     1981 taatatttca tatgaacaaa gcgaagagat atcatctctg ttgcacactg taaatgaatt
     2041 agaacgcatt ggcgataatt gtgaatcact actaaaacta ctaaggcgaa aatatgagca
     2101 aaatattcaa ttttctgatg aagcagtaaa gggtatagat gagattgcat caaaagtacg
     2161 tgagttcatg gttcttatca atcaaaatat aactgttcgt aacaaagata ttatggctga
     2221 ttctaaattc ctggaagata gaattgatga attgcgaaac gagttacgga agggacatgt
     2281 caacagacta aatgaaggtg tttgcaatgt tgatgcgggg ctaatgttta ttgacatgct
     2341 atcaaaattt gaaaaaattg gcgatcatgc ttttaacgtc gctgaaagca tttcaggggt
     2401 tagaatattc taacgctatc ttctttattt ttttaataat ctgtgctgga ttatgcggaa
     2461 tgtatacttc aatgttatct gaatactgta tgccaaattt ttcagtttgc ttatgatgcg
     2521 gcattaaaat atccagtgta aatcccttta ttgcactgcc ggtatcaagc gccacataat
     2581 agttattgtt atactttact attgaaccaa gcggtatgat agaacggtca acggcaacaa
     2641 cattgatagt attatcaagc aatgtggtta gctttaatcc gccaattgcg gcactattgg
     2701 tgtaatcaac aacttttgta ccggtaatac gtttattaca gcagcttccc ggacaatagg
     2761 cggttatttt ccatgtaaca cccttatatt tttcctgggt gttcgatagt tctatccatg
     2821 gctcctgcct tagcaatata tcattgtgat aggtaaatgc catcaaagct aacccaccga
     2881 tgaatagcat tcctataaat aatcgtttca taatttattt tatactataa ttagtctata
     2941 caattaataa taattatact gcattataat gttatttcaa gacaacgttt agcatatcct
     3001 ctgcaagtca atcatttttt aatgtgacat aaaataatgt tgacaaattt tattaattgt
     3061 tttatgtagt agagtcaata tattggcttt atctttattg cataaaatag caattccttt
     3121 aaaaaaaaga ggtgcaatgc gatgataggg aatattgcaa cgctggttat gtgtttggca
     3181 atgatacttt ttttccggcg tcttgaccgt tctaatttgc gaattacaaa actaaaacga
     3241 tatacatcca aagttctgga cgattttaaa aaattaacgc aggatgaaga acgcaaattc
     3301 agtgatgcaa ccatagagct tgatattatg ttaaaaaaag cacaggcgtt aagtactcat
     3361 ctcagttcct caatttcagg tatagaagaa aaattaaaag ggcttgatat tgaaaaaaca
     3421 aatttaaaga aggttgaaga agatttaaaa gttattt
//
LOCUS       DAOOJH010000175         3450 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21128_length_3450_cov_9.429455, whole genome shotgun sequence.
ACCESSION   DAOOJH010000175 DAOOJH010000000
VERSION     DAOOJH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21128_length_3450_cov_9.429455"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLJ75_12595"
     CDS             complement(<1..1061)
                     /locus_tag="PLJ75_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPD06093.1"
                     /translation="MVNLQAKGFDALATSDTVALVCTQEQVASNAFIPEQLHDVFALI
                     DIKTFKAKPNSYYYNALKNKPAILLVGLGPQNNVSAETLRNAASTIVQACQDKGLTQI
                     HIVVPSLTAVPDGARCIAEGVFLSNYNFNRYKTNNDESAPLLQKAILVCDNPQQLSPM
                     LKEVAVVCSNTLLCRDLVNETTENANPVTIAKLAKKIAAQHAIQCTVYTKKDITKMKM
                     GLLLAVSRGSIYPPYCIVMRYRGAPSKKESIAIVGKGITFDTGGINLKPTGHIETMRT
                     DMAGAATCIYAIAAVAQMKLLMNVYVVIPLAENMISHTAYKPGDVFTAYNGKTVEILS
                     TDAEGRLILADALSFTEKT"
     assembly_gap    1239..1338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1339..2991)
                     /locus_tag="PLJ75_12600"
     CDS             complement(<1339..2991)
                     /locus_tag="PLJ75_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HPD06094.1"
                     /translation="MSKTSSRIIQIIESQKGFFTPQDIVASLLFSKTKNKKSKKSNKP
                     PHTDIQKTADSIHNAIATLYHCGFLVKQKARFKVNHPFSFNGTFIHNKKGDGIVYFND
                     TEVHIYREDTNHARNKDIVQVKITDIRRGTLLGTVTTIVSRNKQMFFARCLKPYGKFN
                     ILQLLDVPGNIQVITSEKIKQGMLAIVTVKNTFISNYAECTINTVIADEEQHDVERIV
                     LKHSLPGAHPEYPHLHAIENYVKPHELASRTDYRQLLTVTIDGETAKDFDDAISLEYR
                     RGTYTLYVHIADVSAYVYSNDDIDKEAYARGTSYYLGNTVIPMLPERLSNDLCSLKAG
                     TDRLTLSVMIQFNEQGQYKKHHITRGIINVDRRLTYTQAQEIIHSSKRTPIKTMLMHA
                     KDLSDKLKNLRLSHGRVDLNLADAEMIFDIDSSITDIAFATRLDSHRIIEECMLSANE
                     VVSRILREKNIPALYRIHEPIAEEKLVALTNFLKTLGITLHQEENIGLALQKVIDSVS
                     QKEYEQVVNFIVLKSFMQAYYGSKPLGHFGLGFKDYTHFTSPI"
     gene            complement(3005..3319)
                     /locus_tag="PLJ75_12605"
     CDS             complement(3005..3319)
                     /locus_tag="PLJ75_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06095.1"
                     /translation="MGLLKDASESLLNFSERFLDKTEELAQIARLTMEIKKLEHAINE
                     IYLSIGKHVYNSFESSNTINTDEFITGAIDTINNYKATIVEKQNEIQKVKELYESKHH
                     RY"
BASE COUNT          905 a          670 c          683 g         1092 t
ORIGIN      
        1 atagtctttt cggtaaatga cagagcgtcg gctaaaataa ggcgcccttc cgcatcggtg
       61 cttaaaatct caacggtttt gccgttatat gcggtaaaca catcaccggg tttatacgca
      121 gtatgcgata tcatattttc ggctaacggt atcaccacgt acacattcat gagaagcttc
      181 atctgagcaa ctgccgcgat ggcatagata caggttgctg ctcctgccat atcggttcgc
      241 atggtttcaa tatgccctgt tggcttcagg ttgatgccgc cggtgtcaaa ggttatgcct
      301 ttcccaacta tggcaataga ttcttttttt gaaggtgcac ctctgtaacg cataacaata
      361 caatagggag gatatatgct tccacggcta acggcaagaa gcagccccat cttcatcttt
      421 gtaatatctt tttttgtgta tacggtacat tgtatggcat gctgtgctgc aatttttttt
      481 gcaagtttgg cgatagttac tggattagca ttttctgttg tttcattaac aaggtcacgg
      541 cataaaagtg tattgctgca tacaacggca acctctttca gcatgggcga tagttgttgt
      601 ggattatcgc acaccagaat agccttctgc agcagcggag cagattcgtc attatttgtt
      661 ttataccgat tgaaattgta atttgaaaga aatacacctt cagcaataca gcgtgcacca
      721 tcgggaacag cagtcaaaga gggaacaacg atatgaattt gcgttaaacc cttatcctgg
      781 catgcctgta cgatggtgct tgctgcgttt cgcagggttt cagcacttac attgttttga
      841 gggcccagcc caacaaggag tattgctggt ttgtttttta atgcattgta atagtacgaa
      901 tttggttttg ccttaaacgt tttgatatca atcagtgcaa aaacatcgtg cagttgttct
      961 ggaataaatg cattgctggc aacctgctcc tgtgtacata ccaacgccac ggtgtcggat
     1021 gttgctaatg catcaaaacc ttttgcctgt agatttacca ttcacaccac ctcaaaatcg
     1081 atgcgcataa ggtcaatatc aacaccaaca agcttaacgg taatggtatc gcccaatcta
     1141 aatcgacgtc ccagccgcct gccaatgatt gtgtactcat cttcattgac aaggtaatag
     1201 tcatcggtaa ggaattttaa cggaaccata ccctctatnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnga tgggcgaggt aaagtgagtg taatccttaa agccaagccc
     1381 aaaatgcccc agaggttttg atccatagta tgcctgcata aacgatttta gaactataaa
     1441 attaacaacc tgctcatatt ctttttggga tactgaatca attacctttt gcagtgcaag
     1501 cccaatgttt tcttcctgat gtaaggttat tcccagtgtt ttgaggaagt ttgttaacgc
     1561 taccaatttt tcttctgcta tgggttcgtg tatacggtac agtgcaggga tatttttttc
     1621 gcgcaagatg cgtgatacaa cctcattagc gcttaacata cattcctcaa tgatacggtg
     1681 gctatcaagc cgtgttgcaa atgctatgtc ggtaatggag gaatcgatgt caaatatcat
     1741 ctcagcatca gctaaattaa gatcaaccct tccgtgagaa agccgtaagt ttttaagttt
     1801 atccgataaa tcttttgcat gcatcaacat tgttttgatt ggagtgcgct ttgatgaatg
     1861 gataatttcc tgcgcctggg tataggttaa acgccggtca acattgatta taccccgagt
     1921 tatatgatgc tttttatatt gtccctgttc attaaattga atcatgaccg aaagcgttaa
     1981 cctatccgta ccagctttaa gcgagcacag atcatttgat agccgttcag gcagcatggg
     2041 aatgacagta ttgcccagat aatagcttgt accccgtgca tacgcctctt tatcaatgtc
     2101 atcattgcta taaacatagg ctgatacatc ggcaatgtga acatataatg tgtatgttcc
     2161 acgcctgtat tccagcgata tggcatcatc aaaatctttt gcagtttcgc cgtctatagt
     2221 aacggttagt aattgtcggt agtccgtgcg gctggcaagc tcatggggct ttacatagtt
     2281 ttcaattgca tgcaaatggg gatactcagg atgagctccc ggcaacgaat gcttcaacac
     2341 aatgcgctct acatcatgct gctcttcgtc ggcaatgact gtattgatgg tgcattcagc
     2401 ataattactg ataaaggtat ttttaacagt aactatcgcc agcattccct gttttatttt
     2461 ttcactggtt attacctgta tgttgcccgg aacatccaga agctgcaaaa tgttgaattt
     2521 gccatacggc tttaaacagc gggcaaagaa catctgcttg ttacgcgaaa caattgtggt
     2581 aacagtgcca agcagtgtgc cacgtcgtat gtcggtaatc ttaacctgga caatatcttt
     2641 gttgcgagca tggtttgtat cctcgcggta gatgtgcacc tcggtatcat taaagtacac
     2701 aatgccgtca ccttttttgt tatggataaa tgtaccgttg aagctaaagg ggtggttaac
     2761 cttaaacctg gctttttgct ttacaagaaa accgcaatgg tataatgttg ctatagcatt
     2821 gtggatgctg tcagcagttt tttgaatgtc agtatgcgga ggcttattgc ttttttttga
     2881 ttttttattt tttgtttttg aaaacagcaa agaagctaca atatcctgag gtgtaaaaaa
     2941 acccttttga ctttcaataa tctgtataat gcgcgatgaa gttttagaca tatgctataa
     3001 agttttaata acgatggtgt tttgattcat atagttcctt gactttttgt atttcatttt
     3061 gtttttccac tatggttgct ttataattat ttattgtgtc gatagctcct gttataaatt
     3121 catcagtatt tatagtgttg ctgctttcaa atgagttgta cacatgcttg ccaatagata
     3181 agtatatctc gtttatggca tgttccaatt tttttatttc catggtaagc cttgcaatct
     3241 gcgccagctc ttctgtttta tctaaaaacc gctcgctgaa atttaacaat gattcgctgg
     3301 catcttttaa taaacccata gtaacctcgt tgtagatgta acattaaagc ttcaatatat
     3361 aacatgtgta aaagaatgtc aatatatttt gaataattga ggctctgacc ccattgtttc
     3421 tgaaataatc ttgacaatgg atgagatata
//
LOCUS       DAOOJH010000176         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21689_length_3380_cov_8.176842, whole genome shotgun sequence.
ACCESSION   DAOOJH010000176 DAOOJH010000000
VERSION     DAOOJH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_3380_cov_8.176842"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..864
                     /gene="rpsB"
                     /locus_tag="PLJ75_12610"
     CDS             49..864
                     /gene="rpsB"
                     /locus_tag="PLJ75_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009430879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPD06096.1"
                     /translation="MSVISMKALLESGVHFGHQTRRWNPRMAQFIFTARNGIHIIDLQ
                     KTMQRLKLAYAAMKEIAAKGGRVLFVGTKKQAQAAVKEYAQKCGQFYVSERWLGGLLT
                     NFKTVSRSIQRLKELEQMEITGKWDAETKKEILELTRELERKRKVLEGIKDMDKLPEA
                     LFIIDPKREAIAVQEARKLGIPIFSVVDTNCNPDEIDYPIPGNDDAIRAIALFLEVMS
                     NAIIEGQSAGQVTGEAATATIDFVEEEQSEAKPEKDYAEEYADEIDVYEDDRR"
     gene            912..1511
                     /gene="tsf"
                     /locus_tag="PLJ75_12615"
     CDS             912..1511
                     /gene="tsf"
                     /locus_tag="PLJ75_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPD06097.1"
                     /translation="MADITSDMIKELREKTQAGMLDCKKALIECGGDMEAAVDYLRKK
                     GLAAANKREGRIAKEGIIASYIHNNSKIGVLLELNCETDFVARNQDFQNLAKDLCMQV
                     AAANPLYVSPEDITADVLEREKEIYREQLKESGKPANVIEKIVEGKLQKFYSEVCLLE
                     QEFIKDNKVIIKDLIKNKIATYGENITVGRFVRFQIGQR"
     gene            1553..>2130
                     /gene="pyrH"
                     /locus_tag="PLJ75_12620"
     CDS             1553..>2130
                     /gene="pyrH"
                     /locus_tag="PLJ75_12620"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPD06098.1"
                     /translation="MEEESQPKYKRILLKLSGEALAGQNKVGINPEIVSHIAREVKKA
                     YAMGVDIALVIGGGNIFRGSVGTSIGIERATGDYMGMLGTIINALAVQSVLENMQIPT
                     RVMTAIEMRQVAEPYIRRRALRHLEKRRIVIFAAGTGNPFFTTDTAAALRAIEIGADL
                     LCKATKVDGVYDKDPVKFPEANKMNTVSYMDV"
     assembly_gap    2131..2230
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2254..2811
                     /gene="frr"
                     /locus_tag="PLJ75_12625"
     CDS             2254..2811
                     /gene="frr"
                     /locus_tag="PLJ75_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPD06099.1"
                     /translation="MIKDILDDVKNRMQKSVQALAKDFTTIRTGRANPAIFDNVKVET
                     YGTEMPLNQVATISCPEPRLVVIQPWDRTNLGAIEKAILKSNLSLTPNNDGNIIRIQI
                     PELTEERRKEYVKMIKQKAEDCKVAIRNIRRDGNDMIKDLEKSKDISEDEAKNALDEV
                     QKLTDKFVGEVQMMTDNKEKEIMSL"
     gene            2842..>3380
                     /gene="uppS"
                     /locus_tag="PLJ75_12630"
     CDS             2842..>3380
                     /gene="uppS"
                     /locus_tag="PLJ75_12630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HPD06100.1"
                     /translation="MLTNYKKKIDYAKIPKHIAIIMDGNGRWAKKRGLPRIEGHRRGA
                     EVIERCMDSAIELGIGVVSLYAFSTENWARPKEEINGLWKLLESFFEKKLSTIIEKGV
                     RIQHSGSLSELPPTIQRIIANAVDKSKNNTTIVLNFCLNYGGRHEIIHAVNNWLQQRP
                     SHELSLTPEIFENYLYTAG"
BASE COUNT         1120 a          498 c          744 g          918 t
ORIGIN      
        1 aaataaatgt gttttttaac attgaatctt atatgaggag gtatttcatt gtcagttata
       61 tcaatgaaag cactattgga atcaggggtt cattttggtc atcagacaag aagatggaat
      121 ccccgaatgg cacagtttat ttttactgcg cgtaatggta tccacattat cgatttgcaa
      181 aaaacaatgc agcgtcttaa gcttgcatat gcagcgatga aagagattgc agccaaaggt
      241 ggcagagtac tttttgttgg cacaaagaaa caggctcagg ctgcagtaaa ggaatatgca
      301 caaaaatgcg gacaatttta tgtatctgaa cgctggttag gtggattgtt aactaatttc
      361 aaaactgttt cacgttccat tcaacgattg aaagaactgg agcagatgga aataacagga
      421 aaatgggatg ctgaaactaa aaaagaaatc cttgagctga cgcgcgaatt ggaacgcaag
      481 cgcaaagtac ttgaaggtat caaggacatg gacaaattgc cggaagcatt gttcatcatt
      541 gatcctaaac gtgaagctat tgctgttcag gaggcacgaa agctgggtat tcctattttt
      601 tctgtcgttg ataccaactg taaccctgat gaaatagatt acccaatccc tggcaatgat
      661 gacgcaataa gggcaatagc attattttta gaggtgatgt ctaatgcaat tattgagggg
      721 caatcggcag gacaggtaac tggcgaagct gcgacagcta ccattgactt tgttgaggaa
      781 gagcaatccg aagcaaaacc tgagaaagat tatgctgagg aatatgctga tgaaattgat
      841 gtgtatgaag atgacagacg gtagcctgtg aatgtaataa tattatttta tagtgtataa
      901 ggagtagtaa ggtggctgat atcacaagtg acatgataaa agaattacgt gaaaaaactc
      961 aagcaggtat gcttgattgt aaaaaagcat taatagaatg cggcggcgat atggaagcag
     1021 cagttgatta tttgcgcaaa aaagggcttg ctgctgccaa taagcgcgaa ggtcgtattg
     1081 caaaagaggg gattattgct tcctacattc ataataacag taaaattggt gtgcttttag
     1141 agcttaactg tgaaactgat tttgttgcac gtaatcagga ttttcagaat ctggcaaagg
     1201 atttatgtat gcaggttgct gctgcaaatc ccttatatgt aagtcctgaa gatattactg
     1261 ctgatgtact ggagcgtgag aaggaaatat acagggaaca acttaaagaa agtggtaaac
     1321 cagcaaatgt tatagaaaaa attgttgaag gaaaattaca aaagttttat tctgaagtat
     1381 gccttttaga gcaggaattt attaaagaca ataaggttat tattaaggac ctgataaaaa
     1441 ataagattgc tacctatggc gaaaatataa ctgttggtcg ttttgttcgt ttccagatag
     1501 ggcagcgata gttatgtaca tatgttacgt tgccatacta taggttaaca ggatggaaga
     1561 agaatcacag ccaaagtata aacgaatttt attaaaactc agtggcgagg cgcttgcagg
     1621 acaaaataaa gtgggaatca atcctgagat tgtttcccat attgcccgtg aggttaaaaa
     1681 agcctacgct atgggagttg atattgcgct tgtcattggt ggcggtaata ttttcagagg
     1741 ttcagttggt acatcaatag gcattgaacg agccaccggt gattatatgg gaatgcttgg
     1801 aacgattatc aatgccctgg cagtccagag tgtacttgaa aatatgcaaa taccaacccg
     1861 tgttatgaca gcaattgaga tgcgccaggt agccgagcca tatatacgga gaagagcatt
     1921 acgccatctt gaaaaaagaa gaattgttat ttttgcagca ggtaccggca atccattttt
     1981 tactactgat acagcagcag cactcagagc tattgaaata ggtgctgatc ttctttgtaa
     2041 agctactaaa gttgatgggg tatacgacaa agatcctgtc aaatttcctg aagcaaataa
     2101 aatgaataca gtatcctata tggatgtaat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn attctatttt tggaggaatg tgtatgataa aggatattct tgatgatgta
     2281 aaaaatcgca tgcaaaaaag tgttcaggct ctggcaaaag attttaccac aatacgaacg
     2341 ggaagagcta atccagcgat atttgataat gtaaaggttg aaacgtatgg tactgaaatg
     2401 ccattgaatc aggtagcaac catttcatgc cctgaaccac gattggtagt gattcaaccc
     2461 tgggatagaa caaatttagg agcaatagaa aaagctatat taaaatcaaa tctttcatta
     2521 acacctaata atgatggcaa tattatacgc attcagattc ctgaacttac cgaagaaaga
     2581 agaaaagaat atgttaagat gataaaacaa aaggctgagg actgcaaggt tgcaatacga
     2641 aatatcagac gtgacggtaa tgatatgatt aaagatcttg aaaaatcaaa agatatttcc
     2701 gaggacgaag caaagaatgc actggatgaa gttcaaaagt taaccgacaa gtttgttggt
     2761 gaagtgcaga tgatgacaga taataaagaa aaagagatta tgagcttgta agtattttca
     2821 agctatgtta tactatatat tatgcttact aactataaaa agaaaattga ttatgctaaa
     2881 atcccaaagc atatagctat cattatggat ggcaatggca gatgggcaaa aaaaagaggc
     2941 ctgcctcgaa ttgaagggca tcggaggggc gctgaggtta ttgagcggtg tatggattca
     3001 gctattgaat tggggattgg tgttgtttcg ctttatgcat tttcaacgga gaactgggca
     3061 cgtcctaaag aagagatcaa tggcttgtgg aaacttttag aatctttttt tgaaaagaag
     3121 ctttctacta ttattgaaaa aggtgtaagg attcaacatt ctggcagctt gagtgaattg
     3181 ccacccacca ttcagcgtat tattgccaat gcggtggata aatcaaaaaa taacactact
     3241 attgtgttaa atttttgttt aaactatggt ggcaggcatg agattataca tgcggttaat
     3301 aattggttgc aacaaagacc ttcacatgag ctatcgctta cccctgaaat ttttgaaaat
     3361 tatttataca ctgctggctt
//
LOCUS       DAOOJH010000177         3373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21755_length_3373_cov_6.949970, whole genome shotgun sequence.
ACCESSION   DAOOJH010000177 DAOOJH010000000
VERSION     DAOOJH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21755_length_3373_cov_6.949970"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1031
                     /locus_tag="PLJ75_12635"
     CDS             291..1031
                     /locus_tag="PLJ75_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD06101.1"
                     /translation="MVIRNIQAKSILSVSKVYDYVVNPYVGCQFGCLYCYARYMKKFT
                     GHKEPWGTFVDSKDNAAVLLQKEIAKKKKGLVWISGVCDPYQPLEKKYAITRACLTIL
                     IENDWPVIIQTKSNLVLRDVDIIKNASSCDVGLSITTANDSIRKLFEPQSTAIADRID
                     AIKQLKKHGIRTYVMIAPMLPGCENLIDMLAGSVDYVYVDKMNYHYADSVYKKYALEQ
                     YCDNSYFFSASHEIAYRCKKEGIACSIV"
     gene            1105..>3373
                     /locus_tag="PLJ75_12640"
     CDS             1105..>3373
                     /locus_tag="PLJ75_12640"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPD06102.1"
                     /translation="MKKIIIILVVVIVILGAVIYLFLRTGLPDYTSSLQVPGLASPVT
                     IERNKFAVPTITAQNMDDLYFAWGYVNAQDRLFQMEFTKRVGQGRISEFAGEEALSKD
                     YFLRAVGFYERAKEYTKKLSPYYKRLCQRYADGVNYFIETKGRNAYMILLGMKKEKWE
                     IADSVLVGMMLNWSLAYNMKYELLYHKIVQKIGKEKASLLLNFVPPDTPTVIGDSSGK
                     WLDDAHVSSLLKEFDWLFGCRSASNNWAVAPQLTSHGKAILCSDMQVHQSKLPNDFYL
                     IHVKAGDFEVAGAQVTGLPFIASGFNKHCAWGLTNQGADMVDLFKERIDWKAKRYHYN
                     GKWLPLKNRKEVFAVKGKQPIVKTMYYAGKKPVLTEVFKDLNFDVSLDWTGFDAIALE
                     GFFMINAAKNFDEFMEGAKQIRMSPQNLVYADDKGNIAFRVIGSLPVRKKGTGNFIQD
                     GEKIYSNWNGNIPDDKYPILKNPERGYIATANNLNAADYPYELNGTYAPGYRYENIAT
                     MLRNKKDLDVNYMKKMQTDTHTVLAKKVKAILKKFIQRDALDAYMQKAFDRVIGWDAD
                     SSKDSIGASVYNTFYVRFAYQTLVDELGDTVTEEYVSERYISMERFFEMVEKGSVFFD
                     DVRTNKSETVADIAQRAFKETCQLLCDYFGTDNVEKWQWGKIHSIKFDHILGMSALLK
                     PLVNYGPFPFEGDGETNNRAKFLEVAPPFIADLASAPRIIVKFTPQPEASMMLITGEN
                     EHFLSKHNTDMVDAWL"
BASE COUNT         1078 a          515 c          775 g         1005 t
ORIGIN      
        1 aatgaacctg gttttacatg ggaacaaact gataaagccc aggaattgcg atgatgcaat
       61 ctgtttctat aaaacaatga tacaacaaaa gcgtggattt ccacgctttt tttattgaga
      121 ctttaaacat tgatatggta caattttatt gtgatagtgg tatttttttg atacaggggt
      181 aatgggttat agatatagtg ctgttcaaaa taacgatgat tgcaggtgtg taataataat
      241 gatgagtgcc atcagaaatt acattattta cattattatt gagcaatagt atggttatac
      301 gcaacataca ggcaaaaagt atattgtcgg tttcaaaggt atatgattat gtggtaaatc
      361 cctatgtggg atgccaattt ggttgtttat attgttatgc ccgctacatg aaaaagttta
      421 ccgggcataa ggagccgtgg ggaacatttg ttgatagtaa agacaatgca gcagtgttat
      481 tgcaaaagga aattgcaaag aagaaaaagg gactggtatg gataagcggg gtttgtgatc
      541 cctaccagcc attagaaaaa aaatatgcaa taacacgagc atgcttaacg atactaattg
      601 aaaatgattg gcctgtcata atacagacaa aatcaaatct tgtgctgaga gatgtggata
      661 ttattaagaa tgcatcttca tgtgatgttg gattatccat aacaactgct aatgatagta
      721 ttcggaaact ttttgaaccg caatcaactg caatagcaga caggatagat gcaataaaac
      781 agttgaaaaa acatggcata cgaacgtacg tcatgattgc acctatgttg ccgggttgcg
      841 aaaatcttat tgatatgctt gctggtagcg ttgattatgt atatgtagat aaaatgaatt
      901 atcattatgc agatagtgtg tataaaaaat atgcgctgga acagtattgt gataacagct
      961 attttttttc agcaagtcat gagatagcgt atcgctgtaa aaaagagggg attgcatgtt
     1021 ctattgtcta acatgtattg attgacattt ccatagcatt actctataat ataatagatg
     1081 cagatgaaac aatgggggtt tgcaatgaaa aagataatca ttatactggt agttgttata
     1141 gtgattcttg gtgctgtcat ttatttattt ttgagaacag gcttgccgga ttatacctct
     1201 agtttacaag tacccgggct tgcttctccg gtaacaatcg agcgcaataa atttgccgtg
     1261 ccaaccatta cagcacaaaa catggatgat ctgtactttg cgtggggtta tgtcaatgcg
     1321 caggacaggc tttttcagat ggaattcaca aaacgagttg gacaggggcg catcagcgaa
     1381 tttgctggtg aagaagcttt gagtaaagat tactttttgc gggcggtagg attttatgaa
     1441 agggcaaagg aatataccaa aaaactatcg ccctattata aaaggttgtg tcagcgctat
     1501 gccgatggag ttaactattt tattgaaaca aaaggcagaa atgcttacat gattctactg
     1561 ggaatgaaaa aagaaaaatg ggagatagca gactcagtgc tggttggcat gatgcttaac
     1621 tggagtctgg catataatat gaagtatgaa ctgttgtacc acaaaattgt tcagaaaata
     1681 gggaaagaga aagcctcgtt acttttgaat tttgtaccac ctgatacgcc aacggttatt
     1741 ggcgatagtt ccggaaagtg gctggatgat gcacatgtta gctctttgct gaaagagttt
     1801 gattggcttt ttggctgccg ctctgcttcc aataactggg cggtagctcc gcaactgaca
     1861 tcgcacggga aagcaatact gtgcagcgat atgcaggttc accagtcaaa acttcccaac
     1921 gatttttatc ttatccatgt taaagctggc gattttgaag ttgcgggtgc tcaggtaacg
     1981 ggcttgccgt ttattgcttc aggatttaac aagcattgtg catgggggct taccaatcag
     2041 ggtgccgata tggttgacct ttttaaagaa aggattgatt ggaaagcaaa acgttatcat
     2101 tacaatggaa aatggctgcc attaaaaaat agaaaagagg tatttgctgt aaaagggaaa
     2161 caaccaattg taaagaccat gtattatgca gggaagaaac ctgtgttaac tgaagtattt
     2221 aaagatctga attttgatgt aagccttgat tggaccggtt ttgatgcaat tgcattagag
     2281 ggttttttta tgataaatgc agctaaaaat tttgatgaat ttatggaagg cgcaaaacag
     2341 atcaggatgt cgcctcagaa tctggtttat gctgatgata agggaaatat tgcattcagg
     2401 gtcattggct cactgccggt gcgaaaaaag ggtactggca attttatcca ggatggagaa
     2461 aagatataca gcaactggaa tggaaatata cctgatgata agtatcccat attgaaaaat
     2521 cctgaacgag ggtacattgc cactgcaaat aatctcaatg ctgctgacta tccttatgag
     2581 ttgaacggta cttatgcgcc cggctatcgc tatgaaaata ttgcgacgat gctgcgaaat
     2641 aagaaggatc ttgatgttaa ttatatgaaa aaaatgcaga ccgatactca tacagtgctt
     2701 gcaaaaaagg ttaaagcaat actgaaaaag tttatacaac gtgatgcact tgatgcttac
     2761 atgcaaaagg catttgatag ggtaattgga tgggatgctg atagttctaa agattctatt
     2821 ggcgcctctg tttacaacac attttatgtg cgatttgcgt atcaaactct ggttgatgag
     2881 ttgggtgata cagttacaga ggagtatgta agtgagcgat atatcagcat ggaacgcttt
     2941 tttgagatgg ttgaaaaggg gagtgttttt tttgatgatg tgcggacaaa caaaagtgaa
     3001 accgttgcag atatagcaca acgggcattt aaagaaacat gtcagttgct gtgtgattat
     3061 tttggtacag ataatgttga aaaatggcaa tggggaaaaa ttcatagcat aaaatttgat
     3121 catatacttg ggatgtcagc acttttaaag ccccttgtca attatggtcc gttccctttt
     3181 gaaggtgatg gcgaaacaaa taaccgtgca aaatttcttg aggttgctcc cccgtttatt
     3241 gctgatctag cttcagctcc gcgtattatt gtaaagttta ctccacaacc tgaagcatcc
     3301 atgatgctca ttacagggga aaatgaacac tttttaagta agcataatac tgatatggtt
     3361 gatgcatggc ttg
//
LOCUS       DAOOJH010000178         3368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21794_length_3368_cov_10.752490, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000178 DAOOJH010000000
VERSION     DAOOJH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21794_length_3368_cov_10.752490"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="PLJ75_12645"
     CDS             <1..90
                     /locus_tag="PLJ75_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003461748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPD06103.1"
                     /translation="RKAHIGEIQASIWPEDMVKDCKENNIVLL"
     gene            complement(127..687)
                     /gene="thpR"
                     /locus_tag="PLJ75_12650"
     CDS             complement(127..687)
                     /gene="thpR"
                     /locus_tag="PLJ75_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPD06104.1"
                     /translation="MNRLFIALPIDEAIIQNLLPVHGVLGNFADVVKVVEPSQYHLTL
                     KFIGECEGSIARAIEHEFPQLSLPITRFDYTVKDIGMFPNIKHPAVIWTGIHTDERAL
                     SRLVTSMQMFAKKFGIKEEARSFVPHLTLARIRKGRKLTEKINDTLQPFTSMEFGSAT
                     FNRIVLFSSKLTPQGPVYTPLQEIKL"
     gene            complement(684..980)
                     /gene="gatC"
                     /locus_tag="PLJ75_12655"
     CDS             complement(684..980)
                     /gene="gatC"
                     /locus_tag="PLJ75_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPD06105.1"
                     /translation="MRFDENIINKVCELSRLELTPQEKQEFTMQLEDIIRYVEKINEL
                     DTINVQPADHIVPLSNVFRDDTVHESLPVQEVQKIAPQFEKNHFVVPRIIEEME"
     gene            complement(1014..1655)
                     /locus_tag="PLJ75_12660"
     CDS             complement(1014..1655)
                     /locus_tag="PLJ75_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06106.1"
                     /translation="MKKYIVFVMVLCIGCKTQFGIINTIESESGQSIVSHNADGLTIT
                     VEPFTDDTWFRIAENSQVIDKANMVRLPKLFFFLLLIENKTETPVMIKDIVLRYDTTT
                     TAALTTEALKLSLSSPAYEWIKVDELTVPRRLLYFNGTIQKINFAHDTIPCTLPFVPA
                     GDSHINIIAFTWIPVNVRKFTIAVTAITPDSKKIVECNFIRNEYRTRGKHFLQ"
     gene            1809..>3368
                     /locus_tag="PLJ75_12665"
     CDS             1809..>3368
                     /locus_tag="PLJ75_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HPD06107.1"
                     /translation="MTRLFTTLLLTILLTTCSQKPVLTDLLQKDIDVHYDINALQRLY
                     SRDIYVDPWMQKPYIVKMAYEVDREKQWSKTTRALVLPARYHNPAEVAIPLTWTKGKL
                     TFDCASMGKGRLIVQLLSHKKKMTLGEYTLSPVPFTTMAIAIEKNYSDAVLILNFQSD
                     SEEYAYCFVANAMIKHTDNTTIPNVVFISVDALRADAVHCIVPEYAITPNMDALAKDG
                     AAFVNHCVVANWTRPSTIAMLSSVYGSTTGVNIYYFQVSNQEKKYFYTQSGVVPLPVL
                     FKHHGYITRSIGNNAFILDHSGIGVDVGFDDISEYERQWEDTMDIASEVEQWLADNKD
                     KPFFLFVNFNAPHNAYIPPAQYLEPLKKSIKGVHPWFRRYLGEVAYTDDYIGRIVAVL
                     KSLKLYDNTIIVVTTDHGEVFHEAHSHSPYTDKKSIFTHGQTLFDEEMHVPLIIKLPE
                     KQFTTAVSVPYQVRNIDIAPTVCELAGIPIPQNYQGISLLPYIEGSEHGDRPVYCEGR
                     LMYGVRADGYKY"
BASE COUNT          958 a          655 c          720 g         1035 t
ORIGIN      
        1 cggaaggctc atattggcga gatacaggcg agcatctggc ctgaggatat ggtaaaagac
       61 tgtaaagaaa ataatatagt gctgctgtaa catggttatg gtaaaaaatc agcgatgaag
      121 gttttactat agctttatct cctgtaatgg tgtgtatacc gggccctgtg gtgttagctt
      181 tgaggaaaac agcacaatgc ggttaaatgt tgcactgcca aactccatgg aggtaaatgg
      241 ttgtagcgta tcgttaatct tttcggttaa ctttctgcct ttccggatac gcgccaatgt
      301 aagatgaggc acaaaactgc gggcttcctc cttaatgcca aatttttttg caaacatctg
      361 catactggta accagccgtg atagtgccct ttcatcggta tgtatacctg tccatatcac
      421 tgccggatgt ttgatgttgg gaaacatccc aatgtctttt actgtatagt caaaccgggt
      481 tatgggaagt gaaagctgcg ggaattcatg ttctattgcg cgggcaatac tgccttcaca
      541 ttcgccaata aattttaagg ttaaatgata ctgcgatggt tcaacaacct ttactacatc
      601 ggcaaaattt ccaagtacac catgtaccgg cagcagattt tgtatgattg cctcatcaat
      661 aggaagcgca ataaaaagcc tgttcattcc atctcctcaa taatgcgtgg taccacaaaa
      721 tggttttttt caaactgcgg agcaattttt tgcacctcct gaacaggaag cgactcatga
      781 accgtatcat ccctgaatac attggataat ggtacaatgt ggtcagcagg ctgcacattg
      841 atggtatcaa gctcgttgat tttttcaaca tagcgtataa tatcttctaa ctgcatggta
      901 aattcctgct tttcctgagg tgtcaattct aaacgggaaa gttcgcatac cttgttgatg
      961 atgttttcat caaacctcat ggtttatctc ctccttatag tacataagca tgatcattgt
     1021 aaaaaatgtt tacctcgggt tctatattca tttcgtatga aattacattc aactattttt
     1081 ttactatcag gtgttattgc tgttactgct atggtaaatt tgcgtacgtt gaccggtatc
     1141 catgtgaatg caatgatgtt gatgtgacta tcgcctgcgg gcacaaatgg cagtgtacag
     1201 gggatagtat cgtgtgcaaa attaatcttt tgtatagtac cgttgaagta cagtaagcgc
     1261 cggggtacgg taagctcatc aacctttatc cattcataag caggcgaaga aagagatagt
     1321 tttaatgctt cagtggttag ggcagcagtt gttgtggtat catacctcaa aactatgtcc
     1381 tttatcataa caggggtttc agttttattt tcaatcaata gaaggaaaaa aaatagtttt
     1441 ggcaggcgta ccatatttgc tttgtcaata acttgactgt tttcagctat tctaaaccat
     1501 gtatcgtcag taaaaggttc aacggtaatg gtaagtccat cggcattatg tgatacaatg
     1561 gattgtccgc tttctgattc aatagtattg attatcccaa attgcgtttt gcatccaata
     1621 cacagaacca tcacaaacac tatatatttt ttcatttttt atatccacat catggtacat
     1681 actatagggg cgatagttgt tttgtaaagt ttttattgta ccggtaaaaa tcttttttta
     1741 aattatcgtt gcattaattc cccatacatc gatttgatgc atacaataaa tggatgcagt
     1801 ttgcgattat gacacgattg tttacaacgt tgcttttaac aatacttttg acaacatgct
     1861 ctcagaagcc tgtgctcact gatttgttgc aaaaggacat agatgttcac tatgatataa
     1921 acgcgttaca gaggctttac agcagggata tctatgttga cccgtggatg caaaaaccat
     1981 atatcgttaa gatggcgtat gaagttgaca gggaaaaaca atggtcaaag acaacacggg
     2041 cacttgtttt accagctcgg taccataatc ctgccgaggt tgctattcct cttacatgga
     2101 caaaagggaa attaactttt gactgtgcct caatggggaa gggaaggctg attgtacagt
     2161 tactatcgca caaaaaaaag atgacgttag gcgaatatac gctgtcaccg gtgccgttta
     2221 caacaatggc aattgctata gaaaaaaatt attccgatgc tgtactgata ctaaatttcc
     2281 agagtgattc tgaagaatat gcgtactgct ttgttgccaa tgcaatgatt aaacacactg
     2341 ataatacaac aatccccaat gttgtattta tctcggtcga tgcactgaga gcagatgccg
     2401 tgcactgcat tgtgccagag tatgctatca cccctaacat ggatgcgctt gcaaaggatg
     2461 gcgctgcatt cgtcaatcac tgtgtggtag ctaactggac gcgtccttca acaattgcga
     2521 tgctctcttc agtgtatggt tcaaccaccg gagttaatat ctattacttt caggtgtcca
     2581 atcaggaaaa aaaatatttt tatacgcaaa gcggtgtagt gccgctcccg gtacttttta
     2641 agcatcatgg ctatatcacg cgttctattg gcaacaatgc ttttattctg gatcattcgg
     2701 gcattggtgt cgatgtaggg tttgatgata tctcagaata tgagcgacag tgggaggaca
     2761 cgatggatat tgccagtgag gttgagcagt ggcttgctga caataaggat aaaccgtttt
     2821 ttctttttgt aaattttaat gcaccgcata atgcttatat ccctcctgcg cagtatctgg
     2881 aaccattgaa aaaaagcatc aaaggggtgc atccatggtt tagacgctat cttggcgagg
     2941 ttgcctatac cgatgattat attggcagga tagttgctgt tttaaaaagc ttaaagcttt
     3001 acgataacac catcatagtt gtaacaactg accacggtga ggtttttcat gaggcgcata
     3061 gccatagtcc ctataccgac aaaaaatcta tatttaccca cgggcagaca ttgtttgacg
     3121 aggagatgca tgtgccactt atcatcaaac tccctgaaaa acaatttact acggctgtat
     3181 ctgttcccta ccaggtgcgt aatatcgata ttgcccctac tgtttgtgaa ctggcaggca
     3241 ttcccattcc acagaactat cagggcataa gtttactacc gtacattgag gggtcagagc
     3301 atggggaccg tccggtttac tgtgagggca ggcttatgta tggggtcaga gcagatggtt
     3361 ataagtat
//
LOCUS       DAOOJH010000179         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_21955_length_3350_cov_8.335053, whole genome shotgun sequence.
ACCESSION   DAOOJH010000179 DAOOJH010000000
VERSION     DAOOJH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21955_length_3350_cov_8.335053"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1000..3081
                     /locus_tag="PLJ75_12670"
     CDS             1000..3081
                     /locus_tag="PLJ75_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06108.1"
                     /translation="MIDDNSLKALYKEKLQFIFWFLYYLPYTDSLFNEILEIIPWNKE
                     GIDSLFNGSNGFIWPNQWNSEAMNQYKIDFDIIRLHGFPPFGEDYTIPAFLQTSSKED
                     ALLLVIIYIINYSKTVDFSKIDYDFIDTCFKKINTYVKNNFADIIIGYLHFILGPLGI
                     VRHNEFIEKNSLELFFLFYSIKENTIGNILSLYISGLGNDSFPIDGHFDFNNDFHDLK
                     FFLSHHCSDRDIDGILNKLLASHHDELDTIINYEYNKINFNALKHAKKGNINNYTANE
                     KIQLILLFPVRYIIGAINKNIKMLDLNYDKTVKEDRLKVSEAYRGGQFSYYALTEYGL
                     SPKKYCSESLAENTAGIILDTYTSINRLDDLEKIVRKSGGNIYNRTLHHVIQVKPPEL
                     ILSYLKTNPTIIDSMLALLHLDDTFSGYDPSDKEVVDTIYRAIMTFLGKIFPKQIFSI
                     QKYIMETQEYDKTDNKDILKQNISSIGNTIEMILTHIISYLYCIHSVNTTEVRMHEVV
                     IKEIKEHVATAFNALQVKTKNGIYQIDSARWGLNDMNEILKNITNILPQGKNTLAIDN
                     DVFAKIKNLTNSFNTFRINQLRNSGSHYQLPEEQPSVKELQLSIHNLREFIIQIKNDD
                     LYPHFFYFKSLIKDPELPYRVKFMNEYGNESKEYYFTETINFVEGSIYSITDITKNQI
                     SINPIVLEWDL"
BASE COUNT         1184 a          522 c          599 g         1045 t
ORIGIN      
        1 cgccatacgg ctcgtgcgag gacaaagaca ggatgtcttc cgcagcacga tggcagggat
       61 ataatccata tagcgggata aggcacaccg ccgcctgagg tgcgctttgg gggttggtaa
      121 gggggcattt agcgcataaa tgtcccctta cgaaaggata aaacttttac taccaaaaaa
      181 aagttataca tgctctgacc ccatttttca tcactgctgg attaaaggaa ttcagcggag
      241 gcatggatgc cgtagcgcca tacggctcgt gcgaggacaa agacaggatg tcttccgcag
      301 cacgatggca gggatataat ccatacagcg ggataaggca caccgccgcc tgaggtgcgc
      361 tttgggggtt ggtaaggggg tatttagcgc ataaatatcc ccttacgaaa cgataaaact
      421 tttgtcgccc aacaaaagaa aatatgagaa agaaacatga agatatatct aacattcctt
      481 ttaaaaaaaa gtaacgcctg tcattctgag gcgcagacga agaatccctt cttgtataaa
      541 caaaaataat tcaccactgt cgtcaaaaca acaataaaca gccatacagg gaggtatggc
      601 gtatttggat gcaggtgcat gaagccactt tttctcttgt gaaaacaaaa acggctctga
      661 cccatttttt ttttgcaatt cagggtttga attgttagat atgaaatgta cgtaaataga
      721 tagatagata gatagataga tagatagata gatagataga tagatagata atattggcat
      781 tctactcgat tatataaaat ctaattgttg ctaaaaaaag actcatcaat tgtcagcact
      841 ccttcaggat gacataacgt attgtaatgt tattctaatc catgactaat acctcattgt
      901 gtatggtttc ttaaatagaa aagacttgat ttttatacta tatatatcaa attttattaa
      961 taaatactat acacagcata ttgataggat gtaaaagcca tgatagatga taacagttta
     1021 aaggcattgt ataaggaaaa gcttcaattc attttttggt ttttatatta tttaccatac
     1081 accgatagtt tatttaatga gatactggag attatcccct ggaataagga aggtattgat
     1141 agtttgttta atggtagcaa tggctttata tggccaaacc agtggaacag tgaggctatg
     1201 aatcagtata aaattgattt tgatatcata agattacatg gttttcctcc atttggagag
     1261 gattacacaa taccggcttt tttacaaacc tcttcaaaag aggatgccct tttgcttgta
     1321 attatatata tcatcaacta ttcaaaaaca gtggatttta gtaaaattga ttatgatttt
     1381 attgatacct gctttaaaaa aattaatacc tatgttaaga ataattttgc ggacatcatt
     1441 attggttatt tgcattttat tttggggccg cttggcatag tgcgtcacaa tgaatttata
     1501 gaaaaaaatt ctttggaatt attttttctt ttttattcta taaaagaaaa tactattggt
     1561 aacattttat cgttatatat aagtggtttg ggaaatgatt cgtttcctat agatggtcat
     1621 tttgatttta ataatgattt tcatgattta aaattttttt tgagtcatca ttgttctgat
     1681 cgtgatatcg atggtatcct taataagttg ctggcttctc atcatgacga actggatact
     1741 atcataaact atgaatataa taaaataaac tttaatgcat tgaaacatgc aaagaagggt
     1801 aatatcaata actacactgc aaatgaaaag atacagttga tattattatt tcctgtacgg
     1861 tatatcattg gtgctattaa taaaaatata aaaatgttag atctcaatta tgataaaaca
     1921 gttaaagagg acagattaaa ggtgtctgaa gcttatcgtg gagggcaatt ttcgtattat
     1981 gcacttacag aatatggcct atcccctaaa aaatattgtt cagagtcact tgccgaaaat
     2041 actgccggga ttatattgga tacatataca agcatcaata ggcttgatga tctggaaaag
     2101 attgtacgta aaagcggtgg taatatatac aatagaacat tgcatcatgt tatacaggta
     2161 aaacctcccg aattgatact aagttattta aaaacaaatc caacaataat tgattcaatg
     2221 cttgctctat tgcatttaga tgatacattt tctggatatg acccgtctga taaagaagtt
     2281 gtagatacca tatatagagc aatcatgaca tttcttggaa aaatatttcc taaacaaata
     2341 ttttcaatac aaaaatatat catggaaaca caggaatatg acaaaaccga taataaagat
     2401 atattaaaac aaaatatatc ttctattggt aatactattg agatgatact aacccatatt
     2461 ataagctatc tatattgtat ccatagcgtt aatacgacag aagtgcgcat gcatgaggtt
     2521 gttattaaag agattaagga acatgtagca acagcgttta atgcattgca ggtgaagaca
     2581 aaaaatggca tatatcaaat agactctgcc cgttggggac ttaatgacat gaatgaaatt
     2641 cttaaaaata taacaaacat tttaccacag ggcaaaaata cgcttgctat tgataacgat
     2701 gtgtttgcaa aaataaaaaa tttaaccaat agctttaata catttagaat aaaccaatta
     2761 cggaatagtg gaagccatta tcaacttcca gaagaacagc cgtcagttaa agagcttcaa
     2821 ttatcaatac ataatttaag agagttcatc atccagataa aaaatgatga cctgtatcct
     2881 cacttttttt attttaaatc acttattaaa gatcctgaac ttccctatcg ggtaaaattt
     2941 atgaatgaat atggcaatga aagcaaagaa tattatttta ctgaaactat aaattttgta
     3001 gaaggctcta tatattccat aactgatata acaaaaaatc aaatttcaat taatccaatt
     3061 gtgctggaat gggatttgta gtgctataca gcatttatgc aaaaaatgtg cctatctttg
     3121 ttaatacaat ttaatgtaat tacctgtcat atatgtagat gtgcatacta taattttaat
     3181 ttattatagt gaagaatctc gaccttataa aaatacagat tcttcagtcg ctggcgctcc
     3241 ctcagaatga gggaaacggc tctgacccca tttttttatc actgctggat taacgaaaaa
     3301 cagcgaaggc acggatgccg tagcgccata cggctcgtgc gaggacaaag
//
LOCUS       DAOOJH010000180         3290 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_22490_length_3290_cov_8.659969, whole genome shotgun sequence.
ACCESSION   DAOOJH010000180 DAOOJH010000000
VERSION     DAOOJH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22490_length_3290_cov_8.659969"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLJ75_12675"
     CDS             <1..691
                     /locus_tag="PLJ75_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06109.1"
                     /translation="QASDFIWQQISGNWSVVADNKESYLKENRSKAFAFDYSPLINCN
                     AIISTNSSIVFTRLTHTLMLDAPQDTATYFTFFNASDYRQFYAVQLTGNDSCITTLSI
                     IQSDIKDTTLPKQAKGNFIITTLASSDVQLAYGQPYTLTIAIKNAIITVLVDDKQVLS
                     HTLNEKLEGGSIGFASKNCIPIIDNCKVYNGSSVVFEDDFSKESIRKIILKGKKLTPQ
                     EIEEQKKKQNK"
     gene            866..1543
                     /locus_tag="PLJ75_12680"
     CDS             866..1543
                     /locus_tag="PLJ75_12680"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPD06110.1"
                     /translation="MYTIHIFFLFAFGLFIGFVSSVPVGAVQLEVIKKAINGHLKPAI
                     SVALGSVTSDLIYGILVLFGIGNFLHQHNVQIVTYSLGIIVIIALLYRSYREHKHIPP
                     HLKTHIHYKKRTSYLTGFTIAITNPGMIVWWVIGYKLLLDLALFNTMTVPLKITFLIA
                     CCSGLAGYLILMAFMINKLQESISDKLLDRMNIVIMFLLVLVIIYFSVKLISLITNTS
                     TDIIHNF"
     gene            1623..2069
                     /locus_tag="PLJ75_12685"
     CDS             1623..2069
                     /locus_tag="PLJ75_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06111.1"
                     /translation="MDITVTQQFITDLQSLRENVPLEKEFFAFLYDYFIDAGMSMLLS
                     TPHDYCLIYFDLPEAIDDIQPSNVSWLVEKEEKFLSLTMFANGNQIQTYHTFHIAKNP
                     VNAFFLRSLKDSGTITIHFFTLIYGNMYKLKSIAFHLPEVIINQLE"
     gene            2077..2652
                     /locus_tag="PLJ75_12690"
     CDS             2077..2652
                     /locus_tag="PLJ75_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06112.1"
                     /translation="MREEIKEKFEHIKKGEPIPETNSRKKRFSQIFLLVNLIVFLTLL
                     LLIFQKKEHSDYIYQTVLINNIQVRFSIIHSINKPILATITLTSPDRSVLPIKKPFAT
                     ITILHQNTEITSLPVHIDATVLHITPDKPITHTVSIPLDAIIAFGKKHDLLQYKRYAY
                     HSVIPLTVMFTLHTIMDYSTSLQCKCKVTAK"
     gene            2649..3095
                     /locus_tag="PLJ75_12695"
     CDS             2649..3095
                     /locus_tag="PLJ75_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPD06113.1"
                     /translation="MNRPTWDEYFMTIAHDVATRATCIRRKVGAVIVKEKRILSTGYN
                     GVPTGITHCTPETCLRTIYNVPSGERHELCRGLHAEQNAIIQAAYHGVSIANAIIYVT
                     HQPCSICTKMLINAGIKTFIVEHPYNDELAQAMMNEAGIETIVFKK"
     gene            complement(3096..>3290)
                     /locus_tag="PLJ75_12700"
     CDS             complement(3096..>3290)
                     /locus_tag="PLJ75_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06114.1"
                     /translation="KDYADLKKLSNTDTIKKLLQDDIALGGKLGIHATPTIIINNTML
                     KPGIPPAYILEMAIKIEMKQ"
BASE COUNT         1025 a          669 c          560 g         1036 t
ORIGIN      
        1 gcaggcttcg gactttatat ggcagcaaat atcgggaaac tggtcggttg ttgctgataa
       61 caaagaatcc taccttaaag aaaaccgaag caaagcgttt gcctttgatt atagtccact
      121 tattaattgt aacgccatca tatccaccaa tagttccatt gtatttacca ggttaacgca
      181 taccctgatg cttgatgcac cgcaggacac tgccacctat tttacctttt ttaatgcatc
      241 tgattatcgc cagttttatg ctgtgcagct taccggcaac gattcctgca tcacaacgct
      301 tagcattata caatccgaca taaaggatac aacactgcca aagcaggcta agggcaactt
      361 catcatcacc acgcttgcct ccagcgatgt gcagctcgct tacgggcaac catatacgct
      421 gactatcgct ataaaaaatg caattattac ggtgctggtg gatgataaac aggtactatc
      481 gcacacgctt aatgaaaaac tggaaggtgg tagcattggc tttgcatcaa aaaactgcat
      541 tcccatcatt gacaattgca aggtgtataa cggttcaagc gttgtttttg aagatgactt
      601 ttctaaagaa tcaatacgaa agattatatt aaaagggaaa aagctaacac cacaggaaat
      661 agaggaacaa aagaagaaac aaaataaatg acatgtttta actatgtgtt ttgcttctat
      721 ggctcttcta tggttgtcga agcactataa acatacagaa aatatccatt aaaaatgtat
      781 tttgtaaaaa attaaaaaat acttgcaact ttttatacat aatttgtacc gtatagatta
      841 tgatttcatt ctgataaggt atggtatgta tacaattcac atctttttcc tttttgcatt
      901 tgggcttttt attgggtttg tttcttcagt acctgttggt gccgttcaac tggaggttat
      961 aaaaaaagcc attaatggac atcttaaacc ggcaatctct gtggcgctgg gttcggttac
     1021 atcggacctt atttacggca tccttgttct ttttggcatt ggcaactttt tgcatcagca
     1081 taatgtgcag attgttacct actcgttggg catcattgtt atcatagcat tgctataccg
     1141 ttcatatcgc gagcacaaac atatcccacc tcatcttaaa acacatatac attataaaaa
     1201 gcgtacatcg tatttaaccg gctttactat tgccataacc aaccccggta tgattgtatg
     1261 gtgggttatt ggttacaaac ttcttctgga ccttgcactt tttaacacta tgactgttcc
     1321 attgaagata acatttttaa ttgcatgctg cagcgggctt gcaggttacc ttattctgat
     1381 ggcttttatg ataaacaaat tgcaggaatc aatttcggat aagctgcttg accgcatgaa
     1441 catagtcatt atgtttttgc ttgtactggt aataatttat ttcagcgtta aacttattag
     1501 cctgattacc aataccagca ctgatatcat tcataatttt tagggcgata gttactgtta
     1561 atgctttcgg ttttgttcaa tacggttata cgttaatact atatggcagg caatacccaa
     1621 ttatggatat tactgtaaca caacaattta ttaccgattt gcaaagctta cgtgaaaatg
     1681 ttcctcttga aaaagagttc tttgcttttc tgtacgatta cttcatagat gcgggaatgt
     1741 ccatgctttt gtccacaccc cacgattact gtttgatcta ctttgatcta ccggaagcta
     1801 ttgatgatat acaaccttcc aatgtttcat ggcttgtgga aaaagaggaa aaatttttaa
     1861 gcttaaccat gtttgccaat ggcaaccaga tacaaaccta ccatacattc catattgcaa
     1921 aaaatccagt taatgcattt tttttacggt cattaaagga cagcggtaca ataaccattc
     1981 atttttttac attaatatat ggcaacatgt ataaactaaa atcaattgca tttcatcttc
     2041 ctgaagtaat catcaatcaa ttagaatagt aaaaccatgc gtgaagaaat taaagaaaag
     2101 tttgaacata taaaaaaagg ggaacccatc cctgaaacaa attcgcgtaa aaagaggttt
     2161 tcacaaatat ttttgctcgt gaaccttatt gtatttttga cattactatt gcttatcttt
     2221 caaaaaaaag agcattcaga ctacatctat caaactgttt tgataaacaa tatccaggtt
     2281 cgcttttcaa tcattcattc aatcaacaag cccatactgg caaccattac actgacaagc
     2341 cctgacagat cagttttacc cattaaaaaa ccatttgcca caataaccat cctgcaccag
     2401 aatactgaaa ttacctcgct gccagtacac attgatgcaa ctgtattaca tattacgcct
     2461 gataaaccaa tcacccatac cgtttctatt cctcttgatg caattattgc atttgggaaa
     2521 aaacatgatt tgcttcaata caagcgctat gcttatcata gtgtaatccc tttaaccgta
     2581 atgtttacac ttcacacaat tatggattat tcaacgtcat tacaatgtaa gtgcaaggta
     2641 acggcaaaat gaaccgacca acctgggatg aatattttat gaccattgct catgatgttg
     2701 caacacgtgc aacctgcatt cgacgcaagg ttggcgcagt tatagtaaag gaaaaacgta
     2761 ttctttcaac cggttataat ggagttccaa ccggaataac ccattgcacc cctgaaacgt
     2821 gtcttcgcac tatatacaat gtaccatcgg gcgagcgcca tgagctatgc cgcggtctcc
     2881 atgccgaaca aaacgccatc atacaggcag catatcatgg tgtatcaatt gccaatgcta
     2941 ttatctatgt tacccatcag ccctgctcta tctgtacaaa aatgcttatc aatgccggca
     3001 taaagacatt tatcgttgaa cacccctaca acgatgagct ggcacaagca atgatgaatg
     3061 aggcaggtat tgaaacaatt gtgtttaaaa aataattatt gtttcatctc aatctttatt
     3121 gccatttcca gaatatatgc cggtggaata ccgggtttta acatggtatt gttgatgatg
     3181 atggttggcg ttgcatgtat gccaagttta ccacccagtg ctatatcatc ctgtaaaagc
     3241 ttttttattg tatcagtgtt cgatagtttt tttaaatcag cataatcttt
//
LOCUS       DAOOJH010000181         3287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_22519_length_3287_cov_8.803837, whole genome shotgun sequence.
ACCESSION   DAOOJH010000181 DAOOJH010000000
VERSION     DAOOJH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22519_length_3287_cov_8.803837"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLJ75_12705"
     CDS             <1..1134
                     /locus_tag="PLJ75_12705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD06115.1"
                     /translation="TDSDIQNRQNLVMLEKNMTISTLASGIAHEFNNMNAGIQGLIEL
                     LLSQEELSNAGIKDLRTVLRIVKRATHLVDQLSMLAHKKPSKQMLSNIEDIVNDCVSI
                     IKPQFIDAGISIEVINKNKLSELFLDANKITLALMNLLLNARDAMIDSAKKQLSITTY
                     QDKENVYIEIRDTGCGIKAEYLQRIFEPFFTTKGPLGHSNIPGTGLGLSVALGIVEEH
                     NGAIHVESTPGEGTAFKLSLPLNTTEKVDSEIAVNYNSYNFLKKKILIIEDDVEFATI
                     IQRALHAKNAEVFITNSGAEGLNFLDAHGADLVLLDVHLPDMSVWDILKKIQYIANRP
                     NIIIVSGNYLAMNNNYVGIVDYVLLKPFDLDELFTAIKNSLSL"
     gene            1223..2149
                     /locus_tag="PLJ75_12710"
     CDS             1223..2149
                     /locus_tag="PLJ75_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPD06116.1"
                     /translation="MNDKILLIVNPIAGRGRAAKSVPQIERDFNRLGIKYDIVFTMRQ
                     WHAAELAEDAAKKRYKAVVVASGDGTANEVLNGLMLARKKGNNNTAMGQIPVGTGNDF
                     AYGMGLSDDIDECIKVIADYTLRKIDVGFIKGGDYPQGRYFGNGIGIGFDATTGFVAS
                     RIRFLSGMLVYFIAALQTIFIYYNAPRIRLSYNDKETIMEALQISIMNGKRMGGGFHF
                     APNGSPFDGLLDVCMVRSMNQFKIFSMIPYFIKGTQGKRKEVLEDRTTKIAVVAEKGN
                     LPVHCDGETICYAGKELFIQIYPAVLNFITKA"
     gene            2369..2788
                     /locus_tag="PLJ75_12715"
     CDS             2369..2788
                     /locus_tag="PLJ75_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HPD06117.1"
                     /translation="MSNKYTIHFRKLTHYKYQLMENYIHATSITLQQECCIDDFVALK
                     KDGQLHIFKGYAWDGPSGPTIDTKDFMRGSLVHDALYQLMRKKQLPVSYKDYADKLLH
                     DICIEDGMSKFRAWYVYQAVRLFGKGALKPDDDINNT"
     gene            3020..3238
                     /locus_tag="PLJ75_12720"
     CDS             3020..3238
                     /locus_tag="PLJ75_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06118.1"
                     /translation="MIHDSIHEYNENVYTTFLKGDAREESYYQYLAKLVYDISVSIGY
                     TAVSITTLPKKLMQEIQTLECGMGKVII"
BASE COUNT         1117 a          470 c          656 g         1044 t
ORIGIN      
        1 accgatagcg atatacagaa ccgccagaat ctggttatgc tggaaaaaaa catgacaatc
       61 tcaacattgg cgtccgggat agctcatgag tttaataata tgaatgctgg tattcaaggg
      121 ttaatagaac tcttgttgtc acaggaagaa ctatcaaatg ctggaattaa agatcttaga
      181 acagttttac gcattgttaa gcgtgcaaca catctggttg atcagctttc catgctggct
      241 cataaaaaac catctaaaca aatgctctca aacatagaag acattgttaa tgattgcgtt
      301 agtattataa aaccacagtt tattgatgcc ggaatttcta ttgaggtgat aaataaaaat
      361 aaactatcgg aactgttttt agatgctaat aaaattaccc ttgcgctcat gaatttgctt
      421 ctcaatgccc gtgatgcgat gattgattct gcaaaaaaac agttgtcaat tacaacctat
      481 caggataagg aaaatgtata tatagagata agggatacgg gatgtggtat aaaagcggaa
      541 tatctgcaaa gaatttttga acctttcttt actacgaaag gtcctctggg gcattcaaac
      601 attccaggta cggggcttgg actttctgta gcgcttggta tagtggaaga acataatgga
      661 gcaatacatg ttgaaagcac tccgggtgaa gggactgcat ttaagctttc attaccgctt
      721 aataccactg aaaaagtaga ttctgaaata gcggtaaatt ataatagcta taattttttg
      781 aagaaaaaaa tattgataat agaagatgat gttgaatttg ctacaatcat acaaagagca
      841 ttgcatgcta aaaatgccga agtatttata acaaactccg gagctgaggg gcttaacttt
      901 cttgatgcac atggtgctga tctggtattg ctcgatgttc acctgcctga tatgagtgta
      961 tgggatatat taaaaaaaat tcaatatatt gcaaaccgtc ccaatatcat cattgtttca
     1021 ggtaactatc tggcaatgaa taataattat gtaggcattg tagattatgt tttgttaaaa
     1081 ccatttgatt tagatgagct ttttaccgct ataaaaaaca gcctttccct gtaacctcgt
     1141 tttattattg acaaaattta ttaactgttt aatactccac agcaattttt atacctgcaa
     1201 acctataggg gcgatagtta taatgaatga taaaattttg ttaatagtta atccaattgc
     1261 tggaagaggt cgggctgcaa aatctgtacc gcaaattgaa agagatttta atcgtctggg
     1321 tattaagtat gatattgtat ttaccatgcg acaatggcat gctgctgaac tggcagagga
     1381 tgctgcaaaa aaaagatata aagcggtagt tgttgcaagc ggtgatggaa cggcaaatga
     1441 ggtattaaat ggtttaatgc ttgctcggaa aaaaggaaat aataacactg caatgggcca
     1501 gatacctgtg ggaactggta atgattttgc ttatggaatg ggattatctg atgatatcga
     1561 tgaatgtata aaggtaatag ctgattatac actacgcaaa attgatgttg gttttataaa
     1621 ggggggtgat tatccacagg gaagatattt tgggaatggt attggaattg gatttgatgc
     1681 aaccactggt tttgtggcaa gtagaatcag atttttgagc ggtatgctgg tgtatttcat
     1741 tgctgcttta caaacaatat ttatttacta taatgctccc aggattagat tatcgtacaa
     1801 tgataaagaa acaataatgg aagcattaca gatttctatt atgaatggga agcgtatggg
     1861 aggcggtttc cattttgctc ctaatggtag tccttttgat ggtttgcttg acgtttgcat
     1921 ggttagatcg atgaatcagt ttaagatttt cagtatgatc ccatatttta taaaaggtac
     1981 tcagggtaaa cgcaaagaag tattggagga tagaactaca aaaattgcag ttgttgctga
     2041 gaagggaaat ctacctgttc attgtgatgg cgaaacaatt tgttacgctg gtaaagaatt
     2101 gtttattcag atttatcctg ctgttctaaa ttttatcact aaggcgtaga tattgaaaaa
     2161 tagtgtatca tccttttcat aactataaaa tatagtgtga tagaaaccaa ttttagtttt
     2221 ctacaagatt aaaaggtgca ctgccataac cattatgcat tatgaataaa gatattactg
     2281 gaatcatcgt tgtatagtgt ttggaataat gtaacggcgg taaaccgccg gataggtttt
     2341 tatagattta ttctttaagg aaaaatgtat gtctaacaaa tatacaatac actttagaaa
     2401 acttacccat tataaatatc aacttatgga aaactatata catgctacat caattacatt
     2461 acagcaggaa tgctgcattg atgattttgt tgcattaaaa aaagacggac aactgcatat
     2521 ctttaaaggg tatgcatggg acgggccaag cgggccaacc attgatacaa aagattttat
     2581 gcgaggaagt ttagtccatg atgcgctata tcaactgatg cgcaagaaac aacttcctgt
     2641 atcgtataaa gattatgcgg ataaactttt acacgatatt tgtattgaag atggcatgag
     2701 caaattcaga gcatggtatg tttaccaggc tgtgaggtta tttgggaaag gtgcactcaa
     2761 acctgatgat gacatcaata atacctgata ttgctttttt gttctggtaa ttattgtaat
     2821 agctactatt tgtagttgct caaaaaaagc aggtaaataa aaaaatagac tattaatttt
     2881 tcatgatgca ataaaaaaat ccttgattat ctgtactgaa aagaaaaatt acaccatata
     2941 agctttgaca tataaaatga agatttgcat tatagttgct tgtattgtta ccaactataa
     3001 ttaactaact ggtattttta tgatacatga tagtattcat gaatataatg aaaacgtata
     3061 tactaccttt ttaaaaggtg atgcccggga agaaagttat tatcagtatc tggcaaaatt
     3121 agtttatgat atttctgtat caattggcta tactgctgtt tcaataacca cattaccaaa
     3181 aaaactgatg caggaaatcc agactttaga gtgtgggatg gggaaagtga ttatataaaa
     3241 aatatttttt aaatattacg gtgtgatgca atgaatatac ttgttac
//
LOCUS       DAOOJH010000182         3255 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_22818_length_3255_cov_9.682188, whole genome shotgun sequence.
ACCESSION   DAOOJH010000182 DAOOJH010000000
VERSION     DAOOJH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22818_length_3255_cov_9.682188"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PLJ75_12725"
     CDS             complement(<1..289)
                     /locus_tag="PLJ75_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPD06119.1"
                     /translation="MARKLRLQLPEHTYHIMSRCIEWRDMMQEEYFKACFVDILNRTK
                     QKYDFKLIAYCIMDNHIHIVIHTTSTGAPIARIVQYIKARFAEMYNKITGRT"
     gene            complement(440..1285)
                     /locus_tag="PLJ75_12730"
     CDS             complement(440..1285)
                     /locus_tag="PLJ75_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HPD06120.1"
                     /translation="MDFKKLSQLILIEQTAFALPFAYLGILFAGGGTLTTWIFASLAL
                     VAARTAGMCFNRLIDAEIDAKNPRTKERALPRGEVTKQEVLIVAVISCVVFIFSSYML
                     NMLCFYLSFLAIVLLVSYSYFKRFSSASHLYLGVVEAAAPIGGYLAATGEFTLIPFIL
                     GCAIMMWIAGLDVVYALLDVDFDRQEGLYSIPSRYGVKTALIISSLLYLLSFSSLIIA
                     GMLTNMKEAYWISVLCVGIIFIYQQMLARRKDIEPAIKEFFKVNSFIAPVLFLGTFID
                     VYFIV"
     gene            complement(1275..2717)
                     /locus_tag="PLJ75_12735"
     CDS             complement(1275..2717)
                     /locus_tag="PLJ75_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HPD06121.1"
                     /translation="MGYKDLKDFINTLEKAGQLKHIIPEVDAYLEITQIADRVSKDGG
                     PALLFEKVKGSQYPVLINAFGSYQRMQMALQCNSFDEIGEKIYSLITMQPPKNFMDKV
                     KALLTLKDIALIMPRVVGTAPCQKHVITQGELLNALPILTCWPKDGGPFITLPIVITK
                     DPETGLQNAGMYRMHKFSNTTTGMHWQYNKDGARHYRKYCHLKKQMPVAVALGGSPSI
                     IYAATAPLPPDVDEMLFAGFLGSEPVELVKAKTVDLLVPAHAEFIIEGYVNPGDETME
                     GPFGDHTGFYSAADIYPVFHVTCITTSDNPVYPATIVGKPPMEDCYMAKATERIFLPF
                     LKMVIPEIADIELPLEGVFHNCALVSIKKEYPAQAKKVINALWGLGQMASTKYVVVFD
                     DDVNLRDYSTVVWKLLNNVDPRRDLMIVEGPLDALDHSAPFANFGGKMGIDATRKTKE
                     EGMGRDWPDEITMRDDIIALVAKRWKEYGF"
BASE COUNT          954 a          754 c          597 g          950 t
ORIGIN      
        1 cggtcctgcc ggttatttta ttatacatct cggcaaaccg tgccttgata tactgcacaa
       61 tgcgtgctat gggcgctcct gtgctggttg tgtggatgac aatatggatg tgattgtcca
      121 tgatgcagta ggctatcagc ttaaagtcat atttttgttt tgttctgttc aatatgtcga
      181 caaagcaggc tttaaagtac tcctcctgca tcatatcacg ccactcgata cagcggctca
      241 tgatgtgata ggtatgctcg ggcagttgta ggcgcaattt ccgtgccatg ggtattcctc
      301 cgtgttgtta taataattga aatccctact attcataacg ccggagatga aaaatattaa
      361 aaagaattga gcattttttt atactttttg tgattttttt aaaaaacact aactataagg
      421 gggtcagagc ttatattttt tatacaataa aatagacatc aataaatgtt cccagaaata
      481 gaacaggagc tataaatgaa tttaccttaa aaaattcttt tattgcaggt tctatatcct
      541 ttcttcgagc taacatctgc tgataaataa aaattatccc aacacaaaga acacttatcc
      601 agtaagcctc tttcatgttt gtaagcattc ctgcaattat taatgatgaa aacgataaaa
      661 gatacaacaa cgatgaaata ataagagcag ttttaacgcc atatcgtgat gggatagaat
      721 acaatccttc ctgcctgtca aaatcaacat ctaacagtgc atacacaaca tcaagacctg
      781 caatccacat catgatggca caacccagta taaacgggat caatgtaaac tctcctgttg
      841 cagcaagata tcctcctata ggagctgccg cctctacaac gcctaaatac agatgcgatg
      901 ccgatgaaaa ccgtttaaag tatgaataac ttaccagaag aactatcgcc aaaaatgaaa
      961 gataaaaaca aagcatattg agcatatacg aagaaaaaat aaataccaca catgaaatga
     1021 ccgctacgat aagtacctcc tgtttggtta cctcacctct gggaagcgct ctttcctttg
     1081 tgcgtggatt ttttgcatct atctctgcat caatcagcct gttaaaacac attccagccg
     1141 tacgtgctgc taccagtgct aatgaagcaa atatccatgt tgttagcgtt ccgccgcctg
     1201 caaataaaat gcctaaatag gcaaatggca atgcaaaagc cgtttgttct atcaatataa
     1261 gctgcgacag ttttttaaaa tccatactct ttccacctct ttgcaaccag tgcaatgata
     1321 tcatcacgca ttgttatctc atcaggccag tcacgaccca taccttcttc ttttgtttta
     1381 cgggttgcat caatccccat tttcccgcca aagttagcaa acggtgctga atggtcaaga
     1441 gcatccagcg gaccctccac aatcatgaga tccctgcgcg ggtcaacatt gtttaaaagt
     1501 ttccatacca ccgtactata atcacgcaga ttaacatcat catcaaaaac tacaacatat
     1561 tttgttgatg ccatctgacc caatccccac agtgcattga taaccttctt tgcctgcgca
     1621 ggatactctt tttttataga tacaagtgca cagttatgaa aaacaccttc aagaggaagc
     1681 tctatatctg caatctccgg aattaccatt ttcaaaaatg gtaaaaatat acgctcggtt
     1741 gccttggcca tgtaacaatc ttccatgggt ggtttcccta cgatggtggc aggataaacg
     1801 ggattatcgc ttgttgtaat acaggttaca tgaaacaccg gatagatatc tgcagcagag
     1861 taaaatcccg tatgatcacc aaatggcccc tccattgttt catcaccggg attaacatat
     1921 ccttcaatga taaactcggc atgggcaggt accaacaaat caactgtttt tgcttttacc
     1981 agttcaacag gctctgaccc taaaaaaccc gcaaaaagca tctcatctac atcaggtggc
     2041 agcggtgctg tagctgcata gattatcgaa gggctgcctc ccagcgctac cgctaccggc
     2101 atctgttttt tcaaatggca atatttccgg taatgacgcg caccatcttt attatactgc
     2161 cagtgcattc cggttgttgt gttactaaat ttatgcattc ggtacatgcc tgcattctgc
     2221 aaaccggttt cagggtcctt tgttataact atcggcaggg ttataaacgg cccaccatcc
     2281 tttggccagc atgtcaaaat gggcagagca ttaagcaact caccctgcgt tattacatgt
     2341 ttctggcagg gagctgtacc aactaccctg ggcataatca gggcaatatc cttcaatgta
     2401 agcagggctt taactttatc cataaaattt ttaggcggct gcatcgtaat gagcgaataa
     2461 atcttttcac caatctcatc aaacgaattg cactgcaacg ccatctgcat gcgctggtaa
     2521 ctgccaaatg cattgataag tacaggatac tgtgaaccct ttaccttttc aaaaagaagt
     2581 gccggcccac catctttgct cacgcggtca gcaatctggg taatttcaag atacgcatca
     2641 acctctggta taatgtgttt aagctgcccg gctttttcta acgtgttaat aaaatctttc
     2701 agatctttat atcccacggt tgcctcttta ttatgtaaat gcacctcatt gtatagcaaa
     2761 attgttaaat gcaagtattt tttcattgta tagtgtgtta tacaattctg tgtcatccgg
     2821 gcgtgccccc gctgacgcgg ggtcgggctg ctgcgggcta tggccacagg tgttttacat
     2881 aacaatgcat ataattatat acccctacct tgtacatcac gatgcgcccc tgcaattggg
     2941 catactatgc ctgcaacacc tgtggctggc tttgccaccc tccgcatccc tcacgcatat
     3001 taatccctat aaacattata tggtacaatt atagtatggt taaaaagtgg ggtcagagcc
     3061 ttaccacata ctcgaattgg ggcatgtcat tgttgctcac tcgattcata taataaaaaa
     3121 ggcaagcaaa cagcgcccgc ctttttatga tacatacaac tacctgttta aacagtcgca
     3181 aagctttcct ctggaatatg taacattgaa atatcctgac tctttattgc ctttgcatac
     3241 gattgtgcat gaggc
//
LOCUS       DAOOJH010000183         3100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_24266_length_3100_cov_5.782266, whole genome shotgun sequence.
ACCESSION   DAOOJH010000183 DAOOJH010000000
VERSION     DAOOJH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24266_length_3100_cov_5.782266"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLJ75_12740"
     CDS             complement(<1..224)
                     /locus_tag="PLJ75_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06122.1"
                     /translation="MIPVHEPKINHDAIDFCEKFIHSKQPKLIFGINEFANAIAEAMD
                     IDGFIDDYTTTTIHLGKPIYKTETIPKNALV"
     gene            complement(221..1360)
                     /locus_tag="PLJ75_12745"
     CDS             complement(221..1360)
                     /locus_tag="PLJ75_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPD06123.1"
                     /translation="MDTTFTIGFIGGGLNSAVGITHKIASQMDGHFKLVTGCFSRHND
                     INIQTAEYFNIDKKRLYFNWKELLEHEKDKIDAIVILTPTPSHYEILEETIKNQYAII
                     CEKALVSSVDEGKKVKQLIHKYKNFLTVTFNYTGYPMIRELKNMINKGLLGDINQIHI
                     EMPQETFIKVDKNGNPIYPQPWRQKDYDIPTVSLDLGVHLHSLIYFLTQKKPLEVVAV
                     ENSFGYFKQVVDNVISIIKYSDNMICNMWYSKSACGHRNGLRLRIFGTYGSAEWYQMK
                     PEKLIVCDNKGNIQIVDRVSNLVEVTCLPRYNRFKAGHPAGFIEAFANLYEDIADTLQ
                     HYQQHEDPPVNYTFDIDIALEGLNLLNTINLSAKEKTFIKLEEVI"
     gene            complement(1365..2297)
                     /locus_tag="PLJ75_12750"
     CDS             complement(1365..2297)
                     /locus_tag="PLJ75_12750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD06124.1"
                     /translation="MKNVYISVVTPVYKCAECLDELYTRLIASLHSITNNFEIIMVDD
                     GSPDNAWHIITSLATRDKRVKGIKLSRNFGQHYAITAGLDFAKGRWVVVMDADLQDQP
                     EEIVKLYNKAQEGYDIVVGKRVERKDTFIKKITSKLFFTIYSYLTDTNIDGSISNFGI
                     YSHKVINSIKSFKEHTRSFGLFAIWVGFKRIEIAIEHSNRKKGKSSYSIKKLFTLALD
                     SIISHSDKPLKLFLTSGFMLSFLSFLYAIFLVIQFYIFSIPVTGWTSLMVSIFFLGGI
                     IIACMGIIGLYLNKIYDEAKNRPLYIIAATTFKE"
     gene            complement(2390..2908)
                     /locus_tag="PLJ75_12755"
     CDS             complement(2390..2908)
                     /locus_tag="PLJ75_12755"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD06125.1"
                     /translation="MQTKKIIDHIHDAVYTRKSNLPFTVIATAMFGSYATDKQNIHSY
                     IDLLVVAEGIHPQLHRRASQIVLLKNMLSIGIPSDIILLTKDECISNFTNHNPLFLDI
                     ACDGIIIVDQDSFLSSLIDETKMYITNNKLQKLVDGWRFPVQYRRTTFLSSASNKDFS
                     RAMLVNKNQKLL"
     gene            complement(2934..>3100)
                     /locus_tag="PLJ75_12760"
     CDS             complement(2934..>3100)
                     /locus_tag="PLJ75_12760"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007475171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD06126.1"
                     /translation="SYADLIHYVEDRPGHDKRYAIDPTKIETELGWKARENFEDSLFT
                     TVQWYVTKYV"
BASE COUNT          977 a          559 c          487 g         1077 t
ORIGIN      
        1 actaaagcgt tttttggtat tgtttcagtt ttataaattg gtttccctaa atgtattgta
       61 gttgttgtat aatcatcaat aaaaccgtca atatccattg cttctgcaat tgcattggca
      121 aactcattaa tcccaaaaat caatttaggt tgctttgaat gtataaactt ttcacaaaaa
      181 tctatcgcgt catgatttat ttttggttca tgtactggaa tcatataact tcttccagtt
      241 ttatgaatgt tttttctttt gccgaaagat ttatcgtgtt taaaaggttt aatccttcaa
      301 gtgcgatatc tatatcaaaa gtataattta caggagggtc ttcgtgctgt tgataatgtt
      361 gcaatgtatc agcaatatct tcatataaat ttgcaaaagc ttcaataaaa cctgcaggat
      421 gtcctgcctt aaaccggtta taccggggta aacatgttac ctcaacaagg ttgcttacac
      481 gatcaactat ctgtatgttc cccttgttat cacatactat aagcttttca ggtttcatct
      541 gataccattc agctgaacca taagtgccaa aaattctcaa tcgcaatcca ttcctgtgtc
      601 cacaggcaga tttactgtac cacatgttgc atatcatgtt atccgaatat ttaataatgc
      661 taataacatt atcaactacc tgtttaaaat aaccaaaaga attttcaacg gcaacaacct
      721 ctaatggttt tttttgtgtt aagaaataaa ttaaactatg cagatgtact cccaggtcaa
      781 gggatacagt aggtatgtca taatcttttt gtcgccatgg ttgggggtat attgggttgc
      841 catttttatc aacttttata aaggtttcct gcggcatttc aatgtgtatt tggttaatat
      901 cccctaataa tcctttgttt atcatatttt ttaattcacg tatcataggg taaccggtat
      961 aattaaaggt tactgttaaa aagtttttat atttatgtat tagctgtttt acttttttac
     1021 cctcatcaac cgaagaaaca agggcttttt cacatattat tgcgtattga ttttttattg
     1081 tctcttctaa aatttcataa tgcgatgggg ttggggttaa aatgacaatg gcatctattt
     1141 tatctttttc gtgctctaaa agttctttcc agttaaaata taatcttttt ttgtctatat
     1201 taaagtattc agcagtttgt atatttatat cattgtgtct gctaaaacag ccagtcacaa
     1261 gtttaaagtg cccatccatt tgtgatgcta tcttatgggt aataccaact gccgaattta
     1321 gtccaccgcc tataaatcca atagtaaagg ttgtatccat gtaattattc cttaaatgtt
     1381 gtagctgcta ttatatataa aggcctgttt tttgcttcat catatatttt attaaggtat
     1441 aaaccaataa tacccatgca ggcaataata ataccaccaa gaaaaaaaat tgaaaccatc
     1501 aagcttgtcc acccggttac agggattgaa aagatataaa attgtataac aagaaaaata
     1561 gcatataaaa atgagagaaa cgataacata aagcctgatg ttaaaaacaa tttcaatggt
     1621 ttatcagaat gtgatataat gctgtcaaga gctaatgtaa ataatttttt aatgctatag
     1681 cttgatttac ctttctttct gttggaatgc tctatagcaa tttcaatcct tttaaaccct
     1741 acccagattg caaacaaacc aaatgaacgg gtatgctctt taaaactctt tatactatta
     1801 ataactttat gggagtatat gccaaaatta ctgatgcttc catctatatt tgtatcagtt
     1861 agatacgagt agattgtgaa aaatagtttt gaggtaatct tttttataaa tgtatctttc
     1921 ctttccactc tctttcccac tacaatatcg tatccttcct gtgccttatt atacaattta
     1981 acaatctctt cgggttggtc ctgtaaatca gcatccatga ccaccaccca tctgcctttt
     2041 gcaaaatcaa gccctgctgt aatggcataa tgctgtccaa aattgcgtga aagctttata
     2101 ccctttaccc gtttatccct tgtagccagc gatgttatga tgtgccatgc gttgtcggga
     2161 ctgccatcgt ccaccatgat aatctcaaaa ttatttgtaa tagagtgtaa tgatgcaatt
     2221 aaacgagtgt acagctcatc cagacactct gcacatttgt aaaccggggt aaccaccgag
     2281 atgtaaacat tcttcataaa caaaacctta tatcattaaa attacatcat aaaaaaatac
     2341 atactgcgtt aatcaacaaa ccaccatgta acaaagttat aagcaactgt tataacaatt
     2401 tttgattttt attgacaagc attgctctgg aaaaatcttt attgctggct gatgacaaaa
     2461 aggttgtgcg acgatactgt acagggaatc tccatccgtc aaccagcttt tgtaatttat
     2521 tgtttgtaat atacatttta gtttcatcta ttaacgacga caaaaagcta tcctgatcaa
     2581 caataatgat tccatcacag gcaatgtcta aaaaaagcgg attatgattg gtaaagttag
     2641 aaatacattc atcttttgta agcagtataa tatctgatgg tatgcctatt gatagcatgt
     2701 tttttaataa aacaatctga cttgcccgtc tatgcagttg cggatgaatg ccttcggcaa
     2761 caacaagaag gtcaatgtac gaatgaatat tctgtttgtc ggtagcataa gaaccaaaca
     2821 ttgcagtagc aattaccgta aaaggaagat tgcttttccg ggtgtacact gcatcatgaa
     2881 tgtgatcaat aattttttta gtttgcatgg taatactata tagttactat cgcttaaaca
     2941 tattttgtaa cataccattg aacggtagta aacaaactat cttcaaaatt ttcacgtgcc
     3001 ttccagccaa gctctgtttc aatctttgtg gggtcaatag catagcgctt atcatgcccc
     3061 ggacggtctt caacatagtg tattaaatcg gcatacgagg
//
LOCUS       DAOOJH010000184         3097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_24299_length_3097_cov_5.780079, whole genome shotgun sequence.
ACCESSION   DAOOJH010000184 DAOOJH010000000
VERSION     DAOOJH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24299_length_3097_cov_5.780079"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2101)
                     /locus_tag="PLJ75_12765"
     CDS             complement(<1..2101)
                     /locus_tag="PLJ75_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPD06127.1"
                     /translation="MAFSYTNSDYNNTFKQNVRIVTSLYEPFVMLKDGKLVGFDIDLI
                     NEICKQNNLTYSITITSFQDIFTQIEKGRADMAIGCIYVTEEREDIFKFSKSYLEGGL
                     VLVVPADSDITTIYELDNKKLGVKKDATGDLFAGKIVQKGYSIEIIRYLDTVDSFKAL
                     VNGRVDAVLNDYLNSVYLLNKYFSGNIKIAKGRWRDIVYGRKGIAYPISNSNQHLYEI
                     INKSINDLQSGNFIDMLYWKWFAIMPPPNIPQRVMIYTGLVAVSIGITCMVFILYNRR
                     KKIKHLSMIEEHFRNLINDLNIAVYIIQDNSIVLSNHEGAHIAGSSIEFIIGHSLDDF
                     FLEYTVINNNNKEIHYSTFKDLSNMLQEGPMVYRARWKRKDGTVLDVLFRIRYVQWMG
                     NSALECIVNNTTDLNRLLVEREKLRGQLLQSQKLEIIGQITGGIAHDFNNILTVILGY
                     ANLLEASVDAKNREVVTKIIEAGNHAKDIVSKILTFARKKVFLEQEMHLHNIIQNGIL
                     LLKTALPKSYTIDIQLHATIDTIVGDTTQIQQMLMNLIINASEAMSEGGTISIETTNV
                     AIEHERIGKVETIHAGNYVLLSIKDTGVGIAEKDIEHIFEPLFTTKEKGSGFGLAIVY
                     GIVKQHRGYIDVVSIPGKGSIFYIYLPVITGNSAEALLKIIDNKKESLPALGGEIVII
                     DDDANIGSLYKESLHLAG"
     gene            complement(2421..2714)
                     /locus_tag="PLJ75_12770"
     CDS             complement(2421..2714)
                     /locus_tag="PLJ75_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06128.1"
                     /translation="MKKLLSVVTVVAIVFLGCAAYANGIAQSSHFTAKHIAGMNDNTG
                     DETAMYDPASTVWLKDGVYVNVSSFMAIDDVEAKASGVTPAPIKAANCRKFLM"
BASE COUNT          964 a          573 c          461 g         1099 t
ORIGIN      
        1 aaccagccag atgcaagctt tccttatata aagaaccaat attagcatca tcatcgatta
       61 tcactatttc accacctaaa gctggtaatg attctttttt attgtcaata attttcaaca
      121 atgcttctgc gctgtttcca gttatgactg gtaaatatat ataaaatatg ctccctttgc
      181 cggggattga tactacatca atatatcctc tatgctgttt aacaatacca taaactatcg
      241 ccagaccaaa gccgcttcct ttttcttttg tagtaaacaa aggctcaaag atatgctcaa
      301 tatccttttc agcaatacct accccggtat ctttaataga tagtaaaaca tagttacctg
      361 catgtatggt ttcaaccttc ccaatacgct catgttctat ggcaacatta gttgtttcaa
      421 tactgattgt tcctccctca gacattgctt ctgaagcatt gatgattaga ttcattagca
      481 tttgctgtat ctgtgtcgta tcgcctacta tggtatcaat agttgcatgt aattgtatgt
      541 caattgtgta gcttttaggc aatgctgtct tcaataatag tataccattt tgaatgatat
      601 tgtgcagatg catctcctgc tccagaaaaa cttttttccg tgcaaaggtt aatatttttg
      661 aaacaatatc cttagcatga ttaccagctt caatgatttt tgttactacc tctctatttt
      721 tggcatcaac actggcttca agcaaattgg catagccaag tataacagtt aatatattgt
      781 taaagtcatg tgcaatgcca cctgttatct gccctattat ctccaatttt tgtgattgta
      841 acagttgccc tcttaacttt tcacgctcca ctaataatct gtttaaatcg gtagtattat
      901 taacaatgca ttcaagagcg ctattgccca tccattgtac atagcgaatc ctaaaaagta
      961 catccagtac tgttccatct tttcttttcc atctggctct gtaaaccata ggaccctctt
     1021 gtagcatgtt actgagatct ttgaaagttg aataatgaat ttctttatta ttattattta
     1081 ttactgtata ttccaaaaag aaatcatcaa gtgaatgccc tatgataaat tcaatggagc
     1141 ttcctgcaat atgtgcacct tcatgattgg acaacactat ggaattatct tgaataatat
     1201 aaacagcaat attaagatca ttgataagat tcctgaaatg ttcctcaatc atggataggt
     1261 gcttaatttt ttttcttcta ttgtatagga tgaataccat acatgttatt ccaatggata
     1321 cggcaaccaa tcctgtatag atcatcactc tttgagggat atttggaggc ggcatgattg
     1381 caaaccattt ccagtataac atgtcaataa aattacctga ctgtaaatca ttgatacttt
     1441 tattgattat ctcataaaga tgttgattgc tgttggatat aggatacgct atccccttgc
     1501 gaccatagac aatatcacgc catctaccct tagcaatttt tatattgcct gaaaaatatt
     1561 tatttaataa atacaccgag tttagataat cattgagcac tgcatcaacc ctgccgttaa
     1621 ccagtgcctt aaatgaatca acagtatcca gatatcgtat aatttcaatg gaataccctt
     1681 tttgtactat tttaccggca aacaaatcac ccgtggcatc tttttttact cctaactttt
     1741 tattgtctaa ttcatatata gttgtaatat cactatctgc tggcacaacc aaaaccaggc
     1801 caccctcaag gtagcttttg gaaaatttaa atatatcctc acgttcttct gtgacataga
     1861 tacagccaat agccatatca gctctgcctt tttcaatctg agtaaatata tcctgaaatg
     1921 atgttatggt tatggaatag gttaagttat tctgtttaca gatttcattg attaaatcta
     1981 tatcaaatcc aaccagttta ccatccttaa gcataacaaa tggctcgtac agtgaagtaa
     2041 caatgcgtac attctgctta aaggtgttgt tgtaatcgct attggtataa gaaaatgcca
     2101 tggtattgca aggatttatt aacagcaata cacaacaata tttgagcaag tgtttcatga
     2161 gcatttttta taatgccaaa atcaaaatgt caacgatatt tatggtatag tgtaatcttc
     2221 tattcctttc cttgtcattg cactttgcga tttatttatg tttatgctct aatagggttt
     2281 atcaggttgt catgagaaat tgagaaatta aattgagaaa ttaaattgag aaattaaatt
     2341 gagaaattat ttgaacaata tgtcaataat tagattaata attataaaaa aaggattcct
     2401 ttacggaatc ccgagaagat ttacattaaa aacttgcgac aattcgcagc tttaataggg
     2461 gctggagtaa caccagaagc ttttgcttca acatcatcaa ttgccataaa actgcttaca
     2521 tttacgtata ccccatcctt caaccatacg gtacttgccg gatcgtacat tgcggtttca
     2581 tcaccggtgt tatcgttcat acccgctatg tgttttgccg taaaatgact ggactgagca
     2641 atgccattag cataggcagc acagccaaga aacacaatag caactactgt tacaactgat
     2701 aacaactttt tcatctgcaa acctccattt aaaattttat taccgcccag gcggtttact
     2761 gtgtatacct gtttgtatgc tacattgatt tttttgtaac atacaaacag ttcattgttt
     2821 aatatattaa tgattataca ttaatatata tttattaatg accgttcatt aatttttgtc
     2881 aagcattttt attatttttt tgtaatacaa atgcggcttt agtaggttgt tttttaatga
     2941 atattttttt taaaaggaaa aatttttctt gactatttag tattttagca tatattaaac
     3001 tgtatgattc tttttaattc ctttttttat aggaggaaat taatgaaatc aaatacctac
     3061 tgcatagata acagatgctc atactggagg aatactg
//
LOCUS       DAOOJH010000185         3082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_24463_length_3082_cov_13.737694, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000185 DAOOJH010000000
VERSION     DAOOJH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24463_length_3082_cov_13.737694"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(573..1967)
                     /locus_tag="PLJ75_12775"
     CDS             complement(573..1967)
                     /locus_tag="PLJ75_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017306063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPD06129.1"
                     /translation="MNAALNREQVDLDATIKLQRKFFLDQKTKDIGFRIESLKKLKKA
                     IIKYEDAIYDALKKDLNKSKFEAYTTEIGFVLEEISYHLKHLKKWAKPTKVRTDLVNF
                     YSRSYIVHEPFGVVLIIAPWNYPFQLLVDPLIGAISAGNCVMLKPANYSKNVTNVIVN
                     MMNETFDREYISVYTGGREAIQEILNHRFDYIFFTGSTYLGRIVMEKASRYLTPVSLE
                     LGGKSPCIVEDDANLDVAARRIVFGKLLNAGQTCIAPDYLFVNSRIKEQLIEKIKKCI
                     ILSYGDNPKLSPDYGRIINDAQFKKLKGLIETGKIIFGGQTDERERYIAPTIMDNVKP
                     DDPIMQEEIFGPIFPIMEYNNLDEVIHFINSREKPLAFYFFTSSRKKEKMVLSQTTSG
                     GGCINDTMVHLTNPRMPFGGVGNSGMGAYHGKFSFDTFSHHRSILAKTTLFDIPLRYP
                     PYKDKLKYAKMVFK"
     gene            2232..>3082
                     /locus_tag="PLJ75_12780"
     CDS             2232..>3082
                     /locus_tag="PLJ75_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPD06130.1"
                     /translation="MSDSTSKTTALHRFFGTGGLALTCIQHALRDATVIRIATAYFEP
                     TGYNLLRTTLQNKEVRLLLGRDERSEEKIEQIVNQFIEDLSSADIENKTQFLQELLHA
                     IQNHYFTINLSKQSCPTYTSLAPKFLYQHAKLYVADRSSVVVTSANLTYHGLVQSREA
                     GIVVTSSDDVEYFVKKFDEYFEEAESIAEELLQKLEELLALYSPYQIYIRSLLLLYQI
                     PEEELIGNLKLPPLTGYQRPIVSRIVRTVDEHRGAMLVASTGLGKTVMAAHISAYMRM
                     NGSINSAL"
BASE COUNT          912 a          610 c          642 g          918 t
ORIGIN      
        1 gtttcaatgg taaatgagaa tttctgtcag gataaaatag agatagattt ccatgactgt
       61 gtgctcgcct gcaacggagt gcgccttcca tccgtggaag gctactggaa gcatacggca
      121 gccgggcagg gaagcccggc gcagtaggat gcgggtgcaa gatagggcag cctgccgcag
      181 acaacactac acgacatcgt tgtcaaggca ggcgaggaag cccgttgtgc acccgccgtg
      241 gaacggatat atccatagca atgaaaaagg cgtttttctt tgcatacttt cttttgtcgc
      301 ccaacaaaag aaagtatgaa gaaggaaatg ctgatgtata atggaatttc attcaataaa
      361 gaataggctg atatacagta actatcgcca aaaaaataaa aatcaacacc aacagcttag
      421 aggggcattg ccataaacaa tcccaaaaca gaaaaaattg aacatgtata tatgcgaatt
      481 ggaagtatgg gggcgtagca tactaccccc gataatgtat tatttggata atgttacagc
      541 gcatgcctga gaccagagcg aaagcgaagc tgttacttga aaaccatttt tgcatacttc
      601 agcttatcct tatagggagg ataccgcagg ggaatatcaa acagcgttgt ctttgccagt
      661 atagaccgat ggtgactgaa ggtatcaaaa ctaaatttgc catggtaggc gcccatgccg
      721 ctgttcccca cgccgccaaa gggcatgcgg ggatttgtca ggtgtaccat ggtatcattg
      781 atacagccgc ctcccgatgt tgtttgggac aagaccatct tttccttttt ccggcttgat
      841 gtaaaaaaat aaaaagcaag aggcttttct ctgctgttga tgaaatggat aacttcatca
      901 agattattat attccatgat gggaaatata ggaccaaata tctcttcttg cataataggg
      961 tcatcaggct taacattatc cataattgtt ggggcaatat aacgttcccg ctcatcggtc
     1021 tggccgccaa aaataatttt accggtttca atgagcccct ttagtttttt aaactgcgca
     1081 tcattgataa tgcgcccgta atcagggcta agctttggat tgtcgccata cgaaaggatg
     1141 atacattttt ttattttttc aatgagttgt tcttttatcc tggaattaac aaataagtaa
     1201 tcaggagcaa tgcaggtttg cccggcattg agcaacttgc caaagacaat cctccgtgcg
     1261 gcaacgtcta gattcgcgtc gtcctctact atgcaggggc ttttgccgcc cagttccaga
     1321 gaaacggggg tgagataccg cgatgccttt tccattacaa ttctgcctag atatgtactg
     1381 cccgtgaaaa aaatataatc aaagcgatga ttgagaattt cctggatagc ctcacgtcct
     1441 ccggtataaa cgctgatata ttctctgtca aaggtttcat tcatcatgtt tacgattaca
     1501 ttcgttacat ttttggaata atttgctggt tttagcataa cacagttgcc tgccgagatg
     1561 gcgcctatca gcggatctac gagtaactga aatggataat tccatggcgc gatgatgaga
     1621 acaacaccaa atggttcgtg cacaatatag cttcgtgaat aaaaattgac aaggtctgtc
     1681 cttacttttg taggttttgc ccatttctta agatgcttta aatggtacga tatttcctcc
     1741 aatacaaaac caatctccgt tgtataagcc tcaaattttg acttgttaag gtcctttttc
     1801 agtgcatcgt agatagcatc ctcgtacttt atgatggctt ttttcaattt tttgaggctc
     1861 tctattctga atccaatatc ctttgttttt tgatcaagaa aaaatttcct ctgcaattta
     1921 atagtggcat caaggtcaac ctgttccctg tttaatgctg cattcatgcg ccctccttct
     1981 tggtatgatg gtatgtaaac tttttaaatc aatcttcctg cattactttt tatcgattgt
     2041 tgttctttat gtcaagtctt taacatgcaa caatgcacag caaaccatcg taaatgcagg
     2101 ctttgcaggt tgcttgccta ccattgagcg ttatacatgt actattatgg ggaagattat
     2161 attttttact gtaaaaaaaa ttaataaaaa tatttgttga aaaatactta ctgcatgtac
     2221 ctgtcattat tatgagcgat agcaccagta aaactacagc attacatcgt ttctttggta
     2281 caggaggatt ggcccttaca tgcatacagc atgcattgcg tgatgctacc gtcatacgta
     2341 tagcaactgc atattttgaa ccaaccggat ataacctgtt gaggactaca ttgcaaaata
     2401 aagaagtacg attgctgtta ggcagggatg aaaggtcaga ggaaaagatt gagcagatag
     2461 taaatcaatt cattgaggat ttatcctcag cagatattga aaataaaaca caatttttac
     2521 aggaactttt gcatgcaata caaaaccatt attttactat taatcttagc aagcagtcat
     2581 gtccaaccta tacatcattg gctccaaagt ttctttacca gcatgccaaa ctgtacgttg
     2641 ctgatcgcag cagtgttgtg gtaacatcgg ctaatttaac ctaccatgga ttggtgcaaa
     2701 gtcgcgaagc gggtatagtg gtgaccagca gtgacgatgt agaatatttt gttaagaaat
     2761 ttgatgagta ttttgaagaa gctgaatcca ttgcagaaga actattgcaa aagttagaag
     2821 aattattagc tttatatagt ccctatcaaa tttatatacg gtcattgctg ttattatatc
     2881 aaatacctga agaggagctt attggtaatc ttaaactccc accgttaaca ggatatcaaa
     2941 ggcctatagt atcccgtatt gtcagaactg tcgatgagca ccggggtgct atgcttgtag
     3001 cctcaaccgg actggggaaa acggttatgg ctgctcatat ttccgcctat atgcggatga
     3061 atggaagtat taattctgcg ct
//
LOCUS       DAOOJH010000186         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_24843_length_3048_cov_7.370865, whole genome shotgun sequence.
ACCESSION   DAOOJH010000186 DAOOJH010000000
VERSION     DAOOJH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24843_length_3048_cov_7.370865"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLJ75_12785"
     CDS             <1..572
                     /locus_tag="PLJ75_12785"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPD06131.1"
                     /translation="MGPGSICTTQVTMAVGRAQATAVYRCSKFARDYNIPTIADGGIA
                     TIGHIAKALSLGASTAMMGSMFAGTEEAPGEYFYENGIRLKRYRGMASLEAMQKGGAK
                     RYFAEDSKIMVAQGVSGAVVDKGPMFEYVPYLVQGLKHAFQDMGIKTIATLHDKLYKE
                     ELRFELRSLSAQIEGGVHTVYSYKEPKYL"
     gene            complement(587..1243)
                     /locus_tag="PLJ75_12790"
     CDS             complement(587..1243)
                     /locus_tag="PLJ75_12790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPD06132.1"
                     /translation="MGIAGVIFDLDGTLLDTIEDLAHSTNTVLARYGFATHDIEEYKQ
                     FVGDGMDMLIRRAIGNSSVSNLNDMVVSLKDEYAKSWFVTTKPYAGIEQVLEELSHKK
                     IKISVLSNKAHEFTVTMVQYFFPTIHFSSIMGLQDGMPPKPNPCGALYVAQIMAVEPE
                     HVAFIGDSGTDIEVAHRSNMYSIAVSWGYRPISLLQQHYPEAIAHTPDDILTIIEKHR
                     "
     gene            complement(1247..2020)
                     /locus_tag="PLJ75_12795"
     CDS             complement(1247..2020)
                     /locus_tag="PLJ75_12795"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD06133.1"
                     /translation="MDITNKRVLLTGGSSGIGKAMIDELHGFNTSIVVGDLNPEAIQH
                     HTNTTAMYCDVSKQKDIDTLFNKAITIMGGVDLCIANAGFAYYEKIESVDYQHIQKIV
                     AVNYIAPIYCLEKMMEINKGKKFSVAFTASAMAKLPLPGYALYSATKAALDGFAHSFN
                     FEKDSSVHLLIVYPIATETKFFATAGNNIPVPFPTQTPEKVAKEVIKGLQKEKRYVFP
                     SKLFRGMMVVNRFLPFVLYGYAKVEHYKLKKWLSVSKGQ"
     gene            2426..>3048
                     /locus_tag="PLJ75_12800"
     CDS             2426..>3048
                     /locus_tag="PLJ75_12800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPD06134.1"
                     /translation="MIQLLFIAIACDTLLHMVKPVNTINKLKKGESVTIVALGDSLTY
                     GWMVDTGYVDYFYDFLKIQYPEATITIFNKGVPGDTADGGLHRVGYDVIPNNPDCVFI
                     QFALNDCVYGYSPDIFGNYIRAIINTIQQNIQCDIVIITSVWFGDSPEAQNAYTFYDM
                     LLTIANEYHLPIAMTHQYWEDAVKKGTPLHELVQFDGVHPTEEGYYI"
BASE COUNT          927 a          589 c          565 g          967 t
ORIGIN      
        1 gtatgggtcc cggatcaata tgcacaacac aggttaccat ggcggtagga cgcgcacagg
       61 caacagcagt gtaccgttgc agcaaatttg cccgagatta taatatacca acaatagcag
      121 atggtggtat tgctaccatt ggacatattg caaaagcatt atcactgggg gcttctactg
      181 ctatgatggg gtctatgttt gcagggactg aagaagctcc tggcgaatat ttttatgaaa
      241 acggcatacg cctcaagcgc tatcggggga tggcatccct tgaagctatg caaaaaggcg
      301 gcgcaaaacg atactttgct gaagattcca aaattatggt agcacagggc gtttccggag
      361 cagttgtaga caaaggacca atgtttgaat atgttcctta tcttgtccaa ggtttgaagc
      421 atgcatttca ggatatgggg ataaaaacta ttgcaacgct tcatgataaa ctgtataaag
      481 aagaattgcg ttttgaactg cgttcgttgt cagcacaaat agaaggtgga gtccataccg
      541 tttattcgta taaagagcca aagtacctgt aaagtgttat tgcacattac ctatgctttt
      601 ctataatggt aagtatatcg tcaggtgtat gagctatcgc ttcgggatag tgttgttgca
      661 gtaacgaaat cggtctgtac ccccagctta ccgcaatact gtacatgttg cttctatgcg
      721 ctacctcaat atctgttcct gaatcaccaa taaatgcaac atgttctggt tcaaccgcca
      781 ttatttgggc tacatacagt gctccgcaag ggtttggttt aggaggcatc ccatcctgca
      841 agcccataat gcttgaaaaa tgtatggtgg ggaaaaagta ttgtaccatg gttacggtaa
      901 actcatgggc tttatttgaa aggacagaaa tttttatttt tttgtgcgat agttcctcta
      961 aaacctgttc aatgcctgca tatggttttg tagtgacaaa ccagctttta gcatactcat
     1021 cttttaagga tacaaccata tcattaagat tcgatactga actattgcct atagccctgc
     1081 gaatcaacat atccatgcca tcgcctacaa attgcttata ttcttctatg tcatgagtcg
     1141 caaaaccata acgagctaac actgtattag tactatgtgc caggtcttca atggtatcaa
     1201 gaagggtacc atccagatca aagataacac cagcaatacc catcaatcat tgaccctttg
     1261 aaactgaaag ccactttttc aatttatagt gctcaacctt tgcatagcca tacagcacga
     1321 aaggcaggaa ccggttaaca accatcatgc ctctgaaaag ctttgaagga aaaacgtatc
     1381 gtttttcttt ttgtaaacct tttattacct cctttgcaac tttttcagga gtttgtgttg
     1441 ggaatggcac tggtatattg ttaccagctg ttgcaaaaaa ttttgtctct gttgcaattg
     1501 gatacacaat caaaagatgg acactgctat ccttttcaaa attgaatgaa tgagcaaatc
     1561 catctaaagc agcttttgta gcagaataga gcgcataacc cggcaaaggg agttttgcca
     1621 ttgctgaagc ggtaaacgca acactaaatt ttttgccctt atttatttcc atcatttttt
     1681 ctaaacaata gatgggagct atatagttta cagcaactat cttttgtata tgctggtaat
     1741 ctacactttc tattttttca taataggcaa atcctgcatt agctatacac agatcaacac
     1801 cacccatgat ggtaattgcc ttattaaaca aggtatcaat atctttttgt ttgctcacat
     1861 cacagtacat ggcagtagtg tttgtatggt gttgtatagc ctcaggatta agatcaccaa
     1921 caactattga tgtgttaaac ccatgcaatt catctatcat tgctttccca atacctgacg
     1981 atccaccggt aagtaataca cgcttattag taatatccat attacacctc aatgattaat
     2041 agtatgttgc cttaactttg ctttataata atactcccat tgcatgtata atgaaaatat
     2101 ttgctaagat ttgcaacgaa ccttattgca atgcatatta aacaaggata tgctataatt
     2161 attcccatac ttttgaatat tcacctaaag atgataaaca cagtacttaa gactcaagat
     2221 taacatctgt aatttttcaa gcatatttac tgtttggtgc ttttaacaac cacatttttc
     2281 tgtattataa acatttttaa aaaatgttta tatcaatact ttgtgctcac aaatccatca
     2341 aaaatgtata cttcattttt tcagaagata gattaccggt aactatcgcc cacatgttta
     2401 ttattttcca tttcttatat tttttatgat tcaattattg tttatagcca ttgcctgtga
     2461 tacattgttg catatggtaa aaccagttaa caccatcaac aagctcaaaa aaggtgaatc
     2521 tgtcactatt gtggcattag gcgattcatt gacctatgga tggatggttg atacaggata
     2581 tgtagattat ttttatgatt ttttaaagat tcaatatcct gaggctacca tcacaatctt
     2641 caataaaggt gttcccgggg atacggcaga tggtggatta cacagggttg gatatgatgt
     2701 gattcccaat aatcctgatt gtgtgttcat acagtttgcc ttaaatgatt gcgtgtatgg
     2761 ctattccccg gatatttttg gcaactatat acgggctatt ataaatacaa tacaacaaaa
     2821 tatacagtgc gatatcgtta tcattacctc agtgtggttt ggtgattctc cagaagcaca
     2881 gaatgcatat acattttatg atatgctgct aaccatagca aacgagtatc atcttcctat
     2941 tgctatgacc catcagtact gggaagatgc agtaaaaaaa ggcacaccat tgcatgagct
     3001 ggtacagttt gatggagtgc atccaaccga agagggatac tatatcat
//
LOCUS       DAOOJH010000187         3036 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_24983_length_3036_cov_5.735659, whole genome shotgun sequence.
ACCESSION   DAOOJH010000187 DAOOJH010000000
VERSION     DAOOJH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24983_length_3036_cov_5.735659"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PLJ75_12805"
     CDS             <1..942
                     /locus_tag="PLJ75_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06135.1"
                     /translation="THITPILQTMLKDGLELSLPFDDYCLINSPGTHRRFCKNFIQLF
                     KESYKAIVTSKTPHCRFIGELEGIYFAALRNTTVEVQAFDNIVKVLTTLTLKPDVETH
                     YSFYGDSFYLQALHTLYNKRIQKGITIGYIEENDRLQVHIINEKGNIFTFFAPKNSKM
                     IFLYHIYGFCTNVLKRIPSSSRESQSSIICYCVQTDRRGGINMVDETGKIHGDYLLNF
                     TKSKALRVQVARHMGNEIFYNFIFPDNVSSGFMTVKELYSVKHKITDLKKTGIPIYSY
                     IGELIFNDLNQKEALLGSSIYFVEKYKLELMLQRIHN"
     gene            complement(949..1494)
                     /locus_tag="PLJ75_12810"
     CDS             complement(949..1494)
                     /locus_tag="PLJ75_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06136.1"
                     /translation="MKVIHVLKRIEMIDQDIRELRKLEKSLQSNKSFTQPIFITIEKQ
                     INILLGERIKLLELQIANPPANLQPEEEEIKEPLVAIPKKKGNKKKEQKLHKSKDALS
                     EPDEEAFSEQEFSMLTQDDIDKKIATMQEKIPSTLDDHDDTDVKLLDIALEKGTINKQ
                     EVETQKKKIRFFKDNFPGGES"
     gene            complement(1590..2171)
                     /locus_tag="PLJ75_12815"
     CDS             complement(1590..2171)
                     /locus_tag="PLJ75_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HPD06137.1"
                     /translation="MVIQHRVEHSIAVVTVQGYVNHTNAVEFEEYISSLTKECSSVII
                     DCSQLEYLSSTGIGALMYAQRVIESGGGVVVLCHCNKEIATLIEIIAIPIKRYDSLDE
                     AMQAVNTAPQRLPNQNISEETKEKIHEDKHYTEEKQKVVPDHGSTTFDHPIVVECPKC
                     KSMVKVYASGKFKCPECNLHFTVDDDRTIYFDV"
     gene            complement(2178..2993)
                     /locus_tag="PLJ75_12820"
     CDS             complement(2178..2993)
                     /locus_tag="PLJ75_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06138.1"
                     /translation="MNEKNIIKFPYLFVAILFISLFILSSSIAIFFKYKELTADITNF
                     VVETHNISKTLITMYSDAAGLAYTTKSTYKLVSLFHQQQSLRGIKEIFFVLDNGTIVA
                     HSDEAKKSELNGNIANDEFTYNIDMILYPHTIKSRQLFINDYFMPDITIPFNKEMRTF
                     IKRFIYKDIDTNSWLVSKAVFINGKSVGTVNCIIVKEPVYQIIDSSFNEIMIIIAIIC
                     GVSFFTSLIAGVIVYTRQKKVYRTMSYDTGLHFHEGRRQFPHTGIREAIPVKE"
BASE COUNT          951 a          554 c          473 g         1058 t
ORIGIN      
        1 acccatatca cccctatttt acaaaccatg ctgaaggatg ggttggaatt atctcttcct
       61 tttgacgatt actgccttat caacagtcct ggaacgcata ggcgcttctg taaaaatttc
      121 attcagcttt tcaaagaatc atataaggcg atagttacca gcaaaacgcc tcattgccga
      181 tttattggtg aacttgaagg tatctatttt gctgcattgc gaaatactac tgttgaggtg
      241 caagcatttg acaatattgt caaagtttta acaacactta ccttaaaacc tgatgttgaa
      301 acacactatt cattttatgg cgattctttt tatcttcagg cattgcatac tctttacaac
      361 aaacgcatcc agaagggcat aacgatagga tacattgaag aaaatgaccg attacaggta
      421 cacataatca atgaaaaagg aaatattttt accttttttg caccaaagaa tagtaaaatg
      481 atttttttat accacatcta tggattttgt accaatgtac tcaaacgtat cccaagctca
      541 tcaagagaaa gtcagtccag catcatttgt tattgtgttc aaacagatcg caggggaggc
      601 atcaacatgg ttgatgaaac tggcaagata cacggggatt atcttcttaa cttcacaaag
      661 tcaaaagctt tacgtgttca ggttgccagg catatgggaa atgaaatatt ttacaatttt
      721 atttttcccg ataatgtatc atcaggtttc atgacagtta aggaactata tagtgtcaaa
      781 cacaagatta ctgatttaaa aaagacgggc ataccaatct attcatatat tggtgaactg
      841 atatttaatg acttaaacca gaaagaagct ttattagggt caagtattta ttttgttgaa
      901 aaatacaaat tggaattgat gctacagcgt atacacaatt aatacaaact atgattcgcc
      961 acccgggaag ttatccttaa agaatcgtat cttttttttc tgtgtttcca cttcctgttt
     1021 gtttattgtt cctttttcca aagcaatatc aagcaatttg acatcggtat catcatggtc
     1081 atctaatgtg gacggtattt tttcctgcat tgtagctatt ttcttatcaa tatcatcctg
     1141 cgtaagcata gaaaattcct gctcactgaa tgcctcttca tccggttctg ataaagcatc
     1201 ttttgattta tgcaattttt gttctttctt tttattgcct ttcttttttg gtatcgcaac
     1261 aagtggttct ttgatttcct cttcttctgg ttgcaaatta gccgggggat tggctatctg
     1321 caattcaagt aactttatgc gttcacccaa caaaatattt atttgctttt ctattgtgat
     1381 aaatattggt tgtgtaaatg atttattgct ctgtaacgac ttttccagtt ttcgcagttc
     1441 acgaatatcc tgatcaatca tctctatacg tttaagcaca tgaataactt tcatagctga
     1501 accccaatta aaagaaatta ataatactgt atgtaatagt gaatctataa ttaaaataat
     1561 gtcaataaac ttttatgatg caatcacttt taaacatcaa aatatattgt tctgtcatca
     1621 tctacagtaa aatggagatt gcattcagga catttaaatt tacccgaagc ataaacctta
     1681 accattgact tgcactttgg gcattcgact actatagggt gatcaaacgt ggttgaacca
     1741 tgatccggca caactttttg tttttcttcc gtatagtgtt tgtcttcatg gattttctct
     1801 tttgtctctt cagagatatt ctggttgggc aaccgttgag gagcagtgtt taccgcttgc
     1861 attgcttcat ccaacgaatc ataccgtttt attggaattg caatgatttc aattaatgta
     1921 gctatctcct tattacaatg gcaaagcaca accaccccac cacctgactc aatcactctt
     1981 tgtgcataca tcaatgcgcc aattcctgtt gaactgagat attctaactg cgaacaatca
     2041 ataataacag aagaacattc ctttgtcaga gatgatatat attcttcaaa ttctactgca
     2101 tttgtatggt taacatagcc ttgaactgta accactgcta tcgaatgttc caccctatgt
     2161 tgaataacca taatagccta ctcctttacc ggtatagctt ctctaatacc agtatgtgga
     2221 aattgtcttc ttccctcatg aaaatgcaat cctgtatcat aagacattgt tctgtatact
     2281 tttttttgcc tggtatacac aatgacaccg gcaatgagtg aagtaaaaaa agatactcca
     2341 cagataatcg ctataattat cataatctcg ttaaaagagc tatcaattat ttggtatacg
     2401 ggttccttga caataatgca atttaccgtt cctactgatt ttccattaat aaaaacagcc
     2461 tttgatacca accagctgtt ggtatcaata tctttataaa taaagcgttt aataaatgta
     2521 cgcatttctt tgttaaaagg aattgttata tcaggcataa aataatcatt tataaaaagc
     2581 tgccttgatt tgatagtatg cggataaagg atcatgtcga tattatacgt aaattcatca
     2641 tttgcaatat taccattaag ctctgacttt tttgcttcat ccgagtgtgc tacaattgta
     2701 ccgttatcca gcacaaaaaa gatttctttt attcccctca gtgattgttg ctgatgaaac
     2761 aatgacacaa gcttatatgt tgattttgtt gtatatgcta aacctgctgc atctgagtac
     2821 attgtaataa gtgttttgga aatattgtgt gtttcaacga caaaatttgt aatatcagcc
     2881 gttaattcct tatatttaaa aaaaatggcg atactacttg aaagtataaa caatgaaata
     2941 aatagtatcg caacaaacaa atagggaaac tttataatgt ttttttcatt cataactatt
     3001 tgaaataatg tttattagta tttatactat attaat
//
LOCUS       DAOOJH010000188         3011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_25242_length_3011_cov_5.424899, whole genome shotgun sequence.
ACCESSION   DAOOJH010000188 DAOOJH010000000
VERSION     DAOOJH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25242_length_3011_cov_5.424899"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLJ75_12825"
     CDS             complement(<1..205)
                     /locus_tag="PLJ75_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06139.1"
                     /translation="MKKKVMVYYSYIVAIPIFLLSFLLSCATMNTGDTRLIDQLKAEN
                     RVCSQNLNLFMRENDVLKAENAKY"
     gene            645..1634
                     /locus_tag="PLJ75_12830"
     CDS             645..1634
                     /locus_tag="PLJ75_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06140.1"
                     /translation="MKLFTKVCLVLVLLFTSSTIFAEWVYVPMSINANKGDVVLSTSP
                     GFIMDLLAILGCYWSHSGMAVDNGFNIRHNTMYVSEIPIEYNKIWFIQTTPKRMNPTR
                     LSNGLPGILTEDIDTAYNATNNFAAAGGAVLKPTAANETAYRQYLNAAADVFNYLKAY
                     YRVNAYVNMYQLDYANYLITGRGNHCSGTCWYANYFSGKTMVVATIPANLVTQCANSL
                     YTSVKNMVRDEAGGFGAFIIDIEGLFGTGADEKIANQIVNTFGFDRATDTSDYWRSYI
                     GAVTATANAPDHLILSSYTNPSGHNVGAQTTSTSYYGQVEPLVITDGYYIEIP"
     gene            complement(1687..>3011)
                     /locus_tag="PLJ75_12835"
     CDS             complement(1687..>3011)
                     /locus_tag="PLJ75_12835"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPD06141.1"
                     /translation="GFYILRNVCAGYTGKVYPVNPKYQELLGIPCYPDIESIPDRVEC
                     LIYFIPARFILDIIRRSAAKGIKAIIIESAGFAEAGKEGAKLQKDAVELAHSLGIRLW
                     GPNCMGYLDGHTRNVFSFVHSEKWKTMMYPGDVTLIVQSGMLSAGFLMSVLERDAIGI
                     SKVCSIGNKCDVNEIDLLEYFVQDDTTGAIGCYLESIVDGKKLLKIAQSTSKPIVILK
                     SGRSEQGKKAALSHTASLAGNTMVVSGAFKQAGIVQVVDLYELIDIVKAFSYIKNYRP
                     EPDKGVAVMTFSGGAGIVTTDLLADYAIPIAQPQEATLQKVQKLYPSWMSASHPLDLW
                     PAIEQNGSEKVYTSVAKALMEDERVDSIIIECFATQHSDPSFWGEIGNMVKQSKKPVL
                     AWAVGEKIMVEQYKKHAEASGIPFFGEIGRCVQIFKALRFHFNKRGSI"
BASE COUNT          909 a          668 c          520 g          914 t
ORIGIN      
        1 tatatttagc attttcagct ttaagcacat cattttcacg cataaaaaga ttcaggtttt
       61 gcgaacaaac cctgttttca gctttaagct ggtcaataag cctggtatca ccagtattca
      121 tggttgcaca tgatagtaaa aatgatagta aaaaaattgg gatagcaaca atgtaactat
      181 aatacaccat aacttttttt ttcattatgt atctcccaaa aaattagaat taattataga
      241 ccatacttca agcacctaac tttgacataa ttggcaaccg tcctctgtaa aaaggattat
      301 cagggtaagg ctggtttcat tttcatccct ctcatcaatg ccacgttatt gaatagtaca
      361 gtatagaatg agtaatatat gcatctgatt caacatcttt tttagtcaca acaaaaaaaa
      421 tatagaaacc actctatgaa aatatatgtc atccaatctg ctcaataatt acttaactat
      481 aaaatataaa aaaataccga ccatatggta tttaacactt gactttttct gacaatgtct
      541 ttatagtgta tccaatgaat agtgttgagt gagcaatcac tcaatttgcc atcgactctt
      601 atatcccgtt catttattaa tttaattata agtttggagg ttatatgaaa ctttttacaa
      661 aagtatgtct tgtgctggtt ttgctgttta ccagttccac catttttgct gaatgggtat
      721 atgtacctat gagcattaat gccaataaag gtgatgtagt tctttcaaca agccctggtt
      781 ttattatgga tttattggca atactgggct gctattggag ccacagtggc atggctgttg
      841 ataacggatt taacatccgc cacaacacca tgtatgtaag cgaaatccca atagaataca
      901 acaagatttg gttcattcaa acaacaccta aaaggatgaa tccaacacga cttagtaatg
      961 gactgcctgg catcctaacc gaagatattg atactgccta taacgcaacg aataattttg
     1021 ctgctgcggg cggagcggta ttaaagccaa cagctgcaaa tgagacagcc taccggcaat
     1081 atcttaatgc cgcagcggat gtttttaatt atctcaaagc gtattatcgc gttaatgctt
     1141 acgttaacat gtaccagctt gattatgcaa actatctcat taccggacgt ggcaaccact
     1201 gctcgggtac ctgctggtat gccaattatt tttcagggaa aaccatggtt gttgcaacca
     1261 taccagccaa tctggtgaca cagtgtgcta actcactgta tacctcagta aaaaatatgg
     1321 ttcgtgatga ggctggaggt tttggtgcct tcatcatcga tattgaaggc ttatttggta
     1381 caggtgctga tgaaaaaata gccaaccaga ttgttaacac cttcggcttc gatagggcta
     1441 ccgatacatc cgattactgg agaagctaca taggtgcggt aacagccaca gcaaatgctc
     1501 ccgatcatct gatactttca tcttatacaa atccatcagg gcacaacgta ggtgcgcaaa
     1561 caacatcaac atcatactat ggacaggttg aacctcttgt cattactgat ggctattata
     1621 ttgaaatccc ttagtagaac acgctgacaa tgcatagaaa aatcccgggt taatcccggg
     1681 atttttttat atgctccctc gcttattgaa atgaaacctc agcgccttga atatctgtac
     1741 acaccgtcca atctcaccaa aaaaaggtat accgctggct tcggcatgtt ttttatattg
     1801 ttcaaccatt atcttctcac caactgccca tgcaagcact ggctttttgg attgttttac
     1861 catattgcct atctcacccc agaacgatgg atccgaatgc tgggttgcaa agcattcaat
     1921 gatgatagaa tcaacccttt catcttccat aagggctttt gctacagacg tataaacttt
     1981 ttccgagcca ttctgttcaa tagcaggcca tagatcaagc gggtgcgaag cgctcatcca
     2041 tgaaggatat agtttttgaa ctttttgcaa tgtcgcttct tgaggctgtg ctatgggtat
     2101 agcataatca gccaataaat cagttgttac aatacctgcg ccgcctgaaa atgtcatcac
     2161 tgcaacacct ttatctggct ccgggcgata gttcttgata tatgaaaaag ctttaacaat
     2221 atcaattaat tcatacagat caacaacctg cactattcca gcctgtttaa aagcccctga
     2281 tacaaccata gtattgcctg ccagactggc tgtatggctc agagcagcct tttttccctg
     2341 ttcacttctc cctgatttaa gtataacgat tggtttgctg gtactttgtg ctatttttaa
     2401 cagttttttc ccatcaacaa ttgattccag atagcaacca atcgctcctg ttgtatcatc
     2461 ctgaacaaaa tactctaaca gatcgatttc attgacatca catttattcc caatagaaca
     2521 taccttgcta atacctattg catcacgttc cagtaccgac attaaaaaac ctgccgaaag
     2581 catgccactt tgcactataa gcgtcacgtc acccggatac atcattgttt tccacttttc
     2641 agaatggaca aatgaaaaaa cattccgggt atgtccatct aaatatccca tacagttggg
     2701 cccccacaac cgtatgccaa ggctatgcgc cagttctacc gcatctttct gcaatttggc
     2761 tccttctttg cctgcctctg caaatcctgc cgattcaatg ataattgcct taatcccctt
     2821 tgctgcactt cttctgatga tatccagaat aaagcgtgcc ggtataaaat agattaaaca
     2881 ttcaactctg tcaggaattg actcaatatc aggataacac gggataccca gtagctcctg
     2941 atatttagga tttaccggat acactttgcc tgtataacca gcacaaacat tgcgcagaat
     3001 ataaaaacca t
//
LOCUS       DAOOJH010000189         2989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_25510_length_2989_cov_9.688480, whole genome shotgun sequence.
ACCESSION   DAOOJH010000189 DAOOJH010000000
VERSION     DAOOJH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25510_length_2989_cov_9.688480"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLJ75_12840"
     CDS             complement(<1..508)
                     /locus_tag="PLJ75_12840"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPD06142.1"
                     /translation="MLDKSIQYIKGIGPKRAALLYNELGLETVEDLLYFLPRKYIDRS
                     NTKSINNCFVNDFVTVMGIVKQVKISGHKKKFLEIVIDDGTDTLSGVFFGGIQYFIKL
                     FEKGDTVLFSGRVDYYTNKQIVHPEYDFIGDSSDTLHTGRIVPIYPSTENLKTNGFNS
                     RFFRKIIYT"
     gene            complement(511..825)
                     /locus_tag="PLJ75_12845"
     CDS             complement(511..825)
                     /locus_tag="PLJ75_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06143.1"
                     /translation="MSTKTKLYIFGSTLLIVIVLGMYFYFKYFFTYEQKNITQRKIET
                     ITGQNLTITVFGYDGKIIKRWYGVQKITTPEDSRNYSYFYTRDGKYVQIPGQVWYIAE
                     EE"
     gene            1030..2592
                     /locus_tag="PLJ75_12850"
     CDS             1030..2592
                     /locus_tag="PLJ75_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06144.1"
                     /translation="MEKRLLLYLLSLIVIMLLTIGCKKEKVMEIEPNDTYTQSNLIAI
                     GKPIDGFINTESDIDFYCLEVLNPCLLDINVSAVKGVNSAFTIWKESEGSIVPLKYVD
                     DLRKSAPERFCGIYVENGRYYISISHGDKDQAVKNTENPYTLLILEESADGFEKEPND
                     TILAANPIQIDIPVRGYYSPAFNKANTNAVNPNREEDWFTFYVEDAPVVCDVELSGVA
                     GIEALLDIVDAQGTVLFSSTSQGIGVSQMAKGIGLSVAGNYYIMVAAKNYNANNDVPY
                     SLICTTRNYDNKTEIEPNNIMESATPMVTEIIYGRIYPDNDIDYYQYMGDSSNDVFYR
                     IEINPPMTLDVVSAVYTKKGEEITSINNNGTGDKEVYPNVKLNAPFYVKVWAKRGQVD
                     MDNSYALRIVPVQMGNLVDSEPNDTKEQANNVQGTLINGYISKKHDIDFYVLHYKGRQ
                     KKYFSIKAPDTIPISFSVTDSLGYIIQSITVKAGSSNTLHEIVDMKGYCIVKPLNEMY
                     DGLYTIEIKDRP"
     gene            2589..>2989
                     /locus_tag="PLJ75_12855"
     CDS             2589..>2989
                     /locus_tag="PLJ75_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06145.1"
                     /translation="MNKRRMLILFFLVLFIASGCKTVPKYEPPIEPIISPTEVNVNFR
                     FIAYDTGITTPDEDKRCYYKIFIDKIDAGRTTIGLESQKKYFEAKLSSNMHLIVVEKW
                     VLDEREGEYKKVNNILQPKPNFFYFETKASS"
BASE COUNT         1014 a          497 c          549 g          929 t
ORIGIN      
        1 ctgtgtaaat aatctttctg aaaaaccgtg aattaaaacc atttgttttt aaattttcgg
       61 ttgagggata tatggggact attctgcctg tatgcaatgt atcagagctg tctccaataa
      121 aatcatattc gggatgcact atctgcttat tggtgtagta atcaactcgt cctgaaaata
      181 aaaccgtatc acctttttca aaaagtttga taaaatactg aatgcctcca aaaaataccc
      241 ctgaaagagt atcagtacca tcatcaataa ctatctccaa aaattttttt ttatggcctg
      301 atatctttac ctgttttaca atgcccatta cagtaacaaa atcattgaca aaacaattgt
      361 taatgctttt tgtattggaa cggtcaatat atttccttgg caaaaaatac agtaaatctt
      421 caacggtttc aagccctaat tcattatata acagtgctgc ccgctttggg ccaatgccct
      481 tgatatattg aatgctttta tcaagcacgg ttactcctct tcagcaatat accagacctg
      541 tccgggaatc tgaacgtact ttccatcccg agtataaaag taagaatagt tccggctatc
      601 ctctggtgtt gtaatttttt gtactccata ccagcgtttg atgatttttc catcataacc
      661 aaatactgta atggttaagt tttgccctgt aatagtttct atctttcgct gagtaatatt
      721 tttttgctca taggtgaaaa aatacttaaa ataaaaatac atcccaagta ctataacaat
      781 aagcaaggtg ctaccaaaaa tatatagttt agtttttgtg ctcatacaag cctctatcaa
      841 aatgggattt gcttatcaat agcatacgat tactctatac atcgtagcaa agcaaagaaa
      901 atgtcaaatc ttaatatatt tcagttaatt aaaaaatgta gcttgacttt atgagtaacc
      961 attgtaacaa tcataatcaa tataaaccat ataatacaag agaaatattt tttaaaaatt
     1021 gagatgaata tggaaaaaag attgttatta tatctactat cgctcattgt cattatgctc
     1081 ctgactattg gatgtaaaaa agaaaaagtt atggagatag aacccaatga tacctatacc
     1141 caatcaaacc tgattgcaat tggtaagcca attgatggat ttataaatac cgaaagtgat
     1201 attgattttt attgtctcga ggtactgaat ccatgcttgt tagacattaa cgtcagtgcc
     1261 gtgaaaggag tgaatagtgc atttactatc tggaaggaaa gtgaaggtag tattgtgcca
     1321 ttaaaatatg ttgatgattt gcgaaagtca gcccctgagc gtttttgcgg tatatatgtg
     1381 gaaaatggaa ggtactatat atcaatctcc catggcgaca aagatcaggc ggtaaaaaat
     1441 accgaaaacc cttacacact gttgatactt gaagaatctg ctgatggttt tgagaaagaa
     1501 ccaaatgata caatccttgc cgcaaatcca atacagatag atatcccggt aaggggctac
     1561 tattcacctg cttttaataa ggcaaatacc aatgcagtta atccaaaccg tgaggaggat
     1621 tggttcacat tttatgtcga agatgctcca gtagtgtgtg atgtggagct atcgggtgtt
     1681 gcggggattg aagcactgct tgatattgtt gatgctcagg gcactgtgct tttttcgtct
     1741 acctcgcagg gtattggtgt aagtcagatg gcaaaaggga ttggattatc tgtagcaggg
     1801 aattactaca ttatggttgc agctaaaaat tataatgcca acaacgatgt tccctacagc
     1861 cttatttgta caactcgaaa ttatgataat aaaaccgaga tagaaccaaa caatatcatg
     1921 gaatcggcaa ctcccatggt taccgaaatt atttatggca ggatttatcc tgacaatgac
     1981 attgactatt atcagtatat gggcgatagt tccaatgatg tattttaccg tatcgagata
     2041 aatccgccaa tgactcttga tgtagtatct gcagtttata ctaaaaaagg tgaagaaata
     2101 acttctataa ataataacgg gacaggtgat aaggaagtat atcccaatgt aaagctgaat
     2161 gcaccttttt atgttaaagt atgggctaag cgcggacagg ttgacatgga taatagctac
     2221 gctttgcgga tagttcctgt tcaaatggga aatcttgttg atagtgaacc taatgataca
     2281 aaagaacagg ctaacaatgt gcagggaacg ttgataaatg gatatatttc aaaaaaacac
     2341 gatattgatt tttatgtttt acactataaa gggcgtcaaa aaaaatattt ttccattaaa
     2401 gcgcctgata ccatacctat atcattttct gttactgatt cattgggata tataatacaa
     2461 tcaattactg tgaaagcagg tagttcaaat acattgcatg agatagttga tatgaaagga
     2521 tactgcattg ttaaaccatt gaatgaaatg tatgacggac tatatactat tgaaataaag
     2581 gatagaccat gaacaaaaga aggatgctca ttttattttt tttggtgtta tttattgctt
     2641 caggatgtaa aacggtgcca aaatatgaac cacctataga gccaatcatc agtccaaccg
     2701 aggtgaatgt caatttcagg tttattgctt atgatacagg aattaccact ccggatgaag
     2761 ataaacggtg ctactataaa atttttattg ataagataga cgctggcagg acaaccattg
     2821 ggcttgaatc gcagaaaaag tattttgagg ctaaactatc atccaatatg catcttatcg
     2881 tagttgaaaa atgggtgctt gatgaacgcg aaggtgaata taaaaaagta aacaatattt
     2941 tgcagccaaa gccaaacttc ttttattttg aaacaaaagc ctcatcatt
//
LOCUS       DAOOJH010000190         2911 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_26412_length_2911_cov_6.602591, whole genome shotgun sequence.
ACCESSION   DAOOJH010000190 DAOOJH010000000
VERSION     DAOOJH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26412_length_2911_cov_6.602591"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="PLJ75_12860"
     CDS             complement(<1..517)
                     /locus_tag="PLJ75_12860"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD06146.1"
                     /translation="MAKKIIIVFVIVLVIAGAIVFGVAWNFSNQLISPQPHTCNNEHF
                     IYCGGASELGIPFEDVSFTNDENLTLRGWFFPAKTKTAIIMVHGITAVRKEGLRWVKA
                     QHTAGYNLLLFDLRNHGESDKSISGMGLKEKDDVIAAVNFLVSQKGIKAIGVFGVSMG
                     ASTAIQAMAKDK"
     gene            795..1922
                     /locus_tag="PLJ75_12865"
     CDS             795..1922
                     /locus_tag="PLJ75_12865"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD06147.1"
                     /translation="MIPIIAIPPLLMAAIAMYVAVSYGIMYSRRRNEPENLWFAIMCG
                     TIALYDIASAMLYISPSPFQAVLYQRFQFALLACFIIAITWFIASLVHIRTNIVYSIT
                     AIMVLFIISGFLLDSSYTLNPYNPYIKKFVFLGATITYNEADPGILYIAQYIAMLVSG
                     TYLMYKLFIWCRYGEAHIKILFVSLLVFLLASINDVCVGKGIYRFIYTIEYAYFIVII
                     SMAYILQSRFITLHKEIEQVTYLFNQKLLEQQKQQKIDDTSKQKCISSSNSDKIQQAV
                     EYIHDNFCFAISREGLAAMVNLHPDTFSRLFKLYTGKSLPDYINELRINYAKVLLAEC
                     NESIVTIAFKTGFESLSTFNRAFHKLLNITPTIYRKNNRKF"
     gene            2083..>2911
                     /locus_tag="PLJ75_12870"
     CDS             2083..>2911
                     /locus_tag="PLJ75_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPD06148.1"
                     /translation="MKSTYILTHDVGTTGNKTCLYRVDNKIKLVNSYLVEYPLYMLPD
                     GGVEQKADDWWDAVCKATKWVMKQSNVAPANIAAMAFCAQMQGSLFIDAEGRALRNPM
                     SYLDQRATKQIEKHLYKGFIKIDKWNALKTIKSLVITGGLAATPKDPLWKYHWVKDNE
                     PEIFEKAYKWLDVKDYLIFRCTGKLGMTVDSANVTFIYDTRKNRLGWHKGLCTMFEVD
                     MKHLPPVFQSTDVIGTITKKAANELGLTPGIPVFGGGGDVPLTLVGSGCTDLYDTNIY
                     "
BASE COUNT          902 a          569 c          515 g          925 t
ORIGIN      
        1 gcttatcttt tgccattgcc tgtattgcag ttgatgctcc cattgataca ccaaatacac
       61 caatcgcttt tattcccttt tgactaacca aaaagttaac cgctgctata acatcatctt
      121 tttcctttaa tcccattccc gatatagatt tgtcactttc accatggttg cgcaagtcaa
      181 atagtaaaag attatatcct gctgtatgct gtgctttcac ccatcgcaaa ccttcttttc
      241 tgacagcggt aataccatgt accatgatga ttgccgtttt agtttttgcc gggaaaaacc
      301 atcctcgtaa tgtaagattt tcatcgttag taaatgacac atcctcaaag ggtatcccca
      361 gctcagatgc tcccccacaa taaataaagt gctcattgtt gcaggtatgc ggttgtggac
      421 tgataagctg atttgaaaag ttccatgcta ccccaaaaac tatcgcccct gctataacca
      481 gtacaataac aaaaactata attatttttt tagccatata tcctccaaaa aatgtttgct
      541 attaaatctg tataatcata taatcgttaa cgatattatc aagcaaaaaa atataaaagg
      601 ggatactatg tggcatttta cacctaccaa tgttcactgg attgatatta cattgatcat
      661 actttattta gcaggcatta ccgttttttg attttatata tacacctttt tttattttct
      721 tgcaaaaaac aataatcact ttttattatt tattgatgaa atcatcatta atgaatgtaa
      781 atggcatatt caccatgata cctataatag ctataccacc tcttcttatg gctgctatcg
      841 ccatgtatgt tgccgtctcg tatggtatta tgtattcacg cagaaggaat gaacccgaaa
      901 acctgtggtt tgccataatg tgcggcacta ttgctcttta cgacattgca tctgctatgc
      961 tctatatatc cccatcacct ttccaggctg tattgtacca gcgctttcag tttgcactac
     1021 ttgcctgttt catcatagca ataacgtggt tcattgcttc actggtgcat attcgtacaa
     1081 acattgttta cagcataacc gccattatgg tattgttcat catatcaggt tttttgctgg
     1141 atagttcata tactctcaat ccatacaatc cttacataaa aaaatttgtc tttttaggtg
     1201 caaccatcac ctacaacgag gctgaccccg gtattcttta tatagcacaa tatattgcaa
     1261 tgcttgtttc tggaacatat ttaatgtata aactttttat ctggtgcaga tatggcgaag
     1321 cccatatcaa aattttattt gtaagtttac tggtattttt gcttgcaagc ataaacgatg
     1381 tctgtgtggg caaaggtatt tatcgcttta tttataccat tgaatatgca tattttatag
     1441 ttattatatc catggcatat atcttgcagt cacggtttat aacattgcat aaagagattg
     1501 aacaggttac ctatttattt aatcaaaaac ttttagaaca acagaaacaa caaaaaattg
     1561 atgatacatc aaagcagaaa tgcatctctt cgtccaactc tgataagatt caacaggctg
     1621 ttgagtatat tcatgacaac ttttgttttg ctatttcacg cgaagggttg gctgccatgg
     1681 ttaatcttca tccagataca ttcagcaggc tttttaaact atatacggga aaatcgcttc
     1741 cggactacat caatgaactg aggattaact atgcaaaggt tttattagca gaatgcaatg
     1801 aatcaatagt aactatcgcc ttcaaaaccg gttttgaaag cctttcaaca ttcaaccgtg
     1861 ctttccataa attactcaat ataaccccaa ctatctatcg caaaaataat cgcaaattct
     1921 gattaatcag atattgcgta gacaatgata caaaaataaa gctattgtgt acgatagttg
     1981 ttatggaatc agtgcccttg tgttaatgtt caagaaaacc tattttgccc atagagcttg
     2041 acaatacgtt tatacgatca tataatcctc tgggggtttg caatgaaatc gacatatata
     2101 ttgactcacg atgttggcac aaccggaaac aaaacatgct tatacagggt tgataacaaa
     2161 ataaagctgg ttaactcata ccttgtggaa tatccgctgt atatgctacc tgatggtggt
     2221 gttgagcaaa aagctgatga ttggtgggat gctgtttgta aagctacaaa atgggtaatg
     2281 aagcaaagta atgttgcacc agctaacata gctgccatgg cattttgtgc gcagatgcag
     2341 ggttctctat ttattgatgc cgagggcagg gcgttgcgaa atccaatgag ctatctggac
     2401 cagcgtgcaa caaagcaaat agagaagcat ctgtacaaag gctttataaa gatagataaa
     2461 tggaacgcat tgaaaaccat taaatcactt gtaatcaccg gtgggcttgc cgcaacacca
     2521 aaagacccgc tatggaaata ccactgggta aaagataatg aacctgaaat ctttgaaaag
     2581 gcgtataagt ggctggatgt taaggattat ttgatattca gatgtacagg aaaattaggc
     2641 atgaccgttg attcggcaaa tgtaaccttt atctacgata cccgcaaaaa tagattgggc
     2701 tggcacaagg gattatgcac tatgtttgaa gttgacatga aacatctgcc gccggtattt
     2761 cagtcaaccg atgttattgg aacaatcaca aaaaaagcag caaacgaact tggattaaca
     2821 cccggcatcc ctgtttttgg cggtggaggg gatgtgcctt taactcttgt aggttctggg
     2881 tgcaccgatt tatatgatac caatatctat g
//
LOCUS       DAOOJH010000191         2905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_26468_length_2905_cov_8.644912, whole genome shotgun sequence.
ACCESSION   DAOOJH010000191 DAOOJH010000000
VERSION     DAOOJH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26468_length_2905_cov_8.644912"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="PLJ75_12875"
     CDS             complement(<1..307)
                     /locus_tag="PLJ75_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPD06149.1"
                     /translation="MDYHTITRCAIDICKQAGAIIVEGYYREHITIDYKSRTNLVTDI
                     DYRSEKFIVHELKKQFPEFDIAAEEGSRERADGEYIWYVDPLDATNNFAHKIPWFCVT
                     "
     gene            631..2892
                     /locus_tag="PLJ75_12880"
     CDS             631..2892
                     /locus_tag="PLJ75_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HPD06150.1"
                     /translation="MKKTIMVIGGGLLQVPVIQTAQKMGLRVIVTDYNPNALGMKYAD
                     IPIVMSTRDIEGSVRVAKKQNEITPISGVLTVGTDASVTVAAVANALNLPGIKFEDAI
                     AATNKIKMRTRFFEHGVPSPHFFPVWSLSDAKKASKVLGFPVVIKPSDNMGARGVVRI
                     DNKNQVADAFAFAKSASPSGELIIEEFMKGHELSIDAVVYNGEVTITGIADRIIEYPP
                     HFVETGHTMPSQLPKEQQDSACEIMKAGIKALGITMGCAKGDIKLTDEGPKIGELAAR
                     LSGGFMSAYTYPLSTGVNLMRAAIEVALGQEPGNLEPLYNRVAIERAIITKPGIVKKI
                     CGLEEALKITGIAEIIINVKPGDKVRKPTSNVEKAGHIIAVADSLQEAEDAVRRCKEC
                     INIEICDESELSMDTINMSARERLRKVCYVCKQCNGKDCASGVPGMGGIGNGSSFKRN
                     SEALLNYKINTRVIHDVTNPDTSTSLFGVHLSFPVMAAPITGAVTNLGGAIDELDYNL
                     AVIKGCMDAGSIAFLGDGADPEKYKIIIEALAHHNGMGIPIFKPRANNEDIVKRIKAA
                     EEVGAVAVGMDIDAIVFKTMELKHQSVKPKSLKELTEIISSTHLPFILKGIMNVRDAA
                     LAVESGAHAIVVSNHGGRVLDEMVGTMDVLEDIVHEVKGYIHIVIDGGFRTGADIVKA
                     LALGAEYVLIGRPVAIAAVGMGSEAVSYYLKTLQTELKKAMILTGCATITDISSDIVR
                     SLNHNVYQPFMGR"
BASE COUNT          854 a          606 c          645 g          800 t
ORIGIN      
        1 tggtaacaca aaaccatggg attttatgag caaaattatt tgttgcatcc agaggatcaa
       61 cataccatat atactcacca tcagcccgtt ccctgcttcc ttcttcagca gctatatcaa
      121 actctggaaa ctgtttttta agctcatgta caataaattt ttcggacctg tagtcaatat
      181 ccgtaaccag atttgttctg cttttataat ctatggttat atgctcacgg taatatcctt
      241 caacaataat tgcccctgcc tgtttgcata tatcaatggc acatcgtgta atggtgtgat
      301 agtccatggt acatttctac tccccgcata ctataatcag atgtccacaa taatcatata
      361 ctcatatacc agctattgta tctataagca gcaatgtttg ttgatagcct ttttactgtt
      421 cagtgtcaaa taatttttta tttgctacac catatacagt aactatcgcc acaatcatta
      481 tttacgtgag agcccacgac attatagtat tgacaataac cctcacaatg caagtaaaaa
      541 ttaccgtcaa gtttttgcaa aaaaaaccaa taatatctat agagaggcaa cccggcaggt
      601 tgtaacttta ctattgtcca gggggaatcc atgaaaaaaa cgattatggt gattggcgga
      661 ggtttattac aggtacccgt tatccagaca gctcaaaaaa tgggattgcg ggtgatagtt
      721 actgattata atcccaatgc cctgggtatg aaatatgccg atattcctat tgttatgagc
      781 acccgtgata ttgaaggttc tgtacgtgtt gcaaaaaaac aaaatgagat aacccccata
      841 tccggtgtcc ttactgttgg caccgatgcc tccgttaccg ttgcagcagt agcaaatgct
      901 cttaacctac ccggcataaa atttgaagat gccatagcag ccaccaataa gataaagatg
      961 cgtacccgtt tctttgagca tggcgttcca tcacctcatt ttttcccggt atggtcgctt
     1021 tctgatgcaa aaaaagcaag taaggtactg ggtttcccgg ttgttataaa gccatcggat
     1081 aatatgggtg cacgcggcgt tgtgcgcatt gataacaaaa atcaggttgc cgatgctttt
     1141 gcctttgcaa aaagcgcttc gccaagcggt gaactaatca ttgaggaatt tatgaaaggt
     1201 catgagcttt ccatagatgc cgttgtgtat aatggcgagg ttaccattac cggcattgct
     1261 gaccgcatca tagaatatcc gccacacttt gttgaaacag gacataccat gccttcacag
     1321 ctaccaaaag agcaacagga cagcgcctgt gaaataatga aagctggtat aaaagcgctg
     1381 ggtattacaa tgggctgtgc aaaaggcgat atcaagttga ccgatgaagg accaaagatt
     1441 ggcgaactgg ccgcccgcct ttcaggagga tttatgtctg catacaccta tccactctct
     1501 accggcgtta acctgatgcg tgccgccata gaggttgcac tgggacagga gcccggaaac
     1561 cttgaaccgc tgtataaccg cgtggcaatt gagcgggcaa ttataacaaa accagggatt
     1621 gtaaaaaaaa tctgcggatt ggaagaagcc ctaaagatta caggtattgc tgagattatt
     1681 atcaatgtga agcccggcga taaagtacgg aagccaacat ccaatgttga aaaagcaggt
     1741 cacatcatag ccgtagccga ttcgctgcaa gaagcagagg atgctgtgcg cagatgcaaa
     1801 gaatgtatca atattgaaat atgcgatgaa tccgaacttt ccatggatac catcaacatg
     1861 tctgcacgag aacgccttag aaaggtatgc tatgtatgca aacagtgtaa tggaaaggat
     1921 tgtgccagtg gcgtacccgg tatgggcggt attggtaacg gctcatcgtt taaacgcaac
     1981 agcgaagcat tgctcaatta caagataaat actcgtgtta tccatgatgt caccaatcct
     2041 gatacctcaa catcgctctt tggagtacat ctgtcattcc ctgtaatggc agcccctatt
     2101 actggagcgg ttacaaacct tggtggtgct atcgacgaac ttgattataa ccttgccgtt
     2161 ataaaagggt gtatggatgc tggcagtatt gcctttttag gggatggcgc agatcccgaa
     2221 aagtataaaa ttattattga agcgcttgcc caccataacg gtatgggtat cccaatcttt
     2281 aaaccgcgcg ccaacaatga ggatatagta aagcgcataa aggcagccga agaggtaggt
     2341 gccgttgctg ttgggatgga tattgatgcc attgtcttta aaaccatgga attgaagcat
     2401 cagtcggtta aaccaaaaag tttaaaagag cttaccgaaa ttatatcatc aacacatctt
     2461 ccctttatac tgaagggtat tatgaatgtc agggatgctg cacttgctgt tgaatcaggt
     2521 gctcatgcca tcgttgtttc caatcatggc ggcagggtac tggatgaaat ggtaggcacc
     2581 atggatgttc tggaggatat tgtccatgaa gttaagggat acatccatat tgttattgac
     2641 ggtggtttta gaacaggtgc tgatattgta aaagcgctgg cactgggtgc tgaatacgtg
     2701 cttatagggc gtcctgttgc tatagcagcg gtaggcatgg gctctgaagc agtatcttat
     2761 tatctcaaaa ccttgcagac agagcttaaa aaggccatga tactgactgg ctgtgctacc
     2821 atcaccgata tcagcagtga tatcgtgcgt tcactgaatc ataatgtgta ccaacctttt
     2881 atggggcgat agttattgtt aagcg
//
LOCUS       DAOOJH010000192         2897 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_26568_length_2897_cov_7.077762, whole genome shotgun sequence.
ACCESSION   DAOOJH010000192 DAOOJH010000000
VERSION     DAOOJH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26568_length_2897_cov_7.077762"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..828)
                     /locus_tag="PLJ75_12885"
     CDS             complement(190..828)
                     /locus_tag="PLJ75_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06151.1"
                     /translation="MNSMTQNPFKKIFEEKYEQLLQHPEMQRVKHFLSNLLTIDDQDI
                     MFVITNELGNTVTLYSSKQNNNPIQSIALKIDSNADSFIPSIVFASAYDSYSIIKSYT
                     KPSNILLPECNIPLYNEVQFGKAIFHELGGCDIDEISDVLFDVASDEEIVETTAFQMA
                     QTLTIIKICELLHDACLLLVQGDRFKKLSITKPFSFFITIDDKEYITSVTID"
     gene            1712..>2897
                     /locus_tag="PLJ75_12890"
     CDS             1712..>2897
                     /locus_tag="PLJ75_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD06152.1"
                     /translation="MKVLLVNPPYPFEEAPSPPFGLMALAAYLEAQGIEVKIEDYIVN
                     RFTPERVSDVLSQFAPDVVGSTGVTMNIKKALSIVKVYKELDKDIVTVLGGPHVTFDA
                     HAILRNYPFVDVIVRGEGEATFHEFLQHLHSISAYGAIKGISYRDKDNDIVHNPIREF
                     IPDINILPFPARHLVELSKYRALGFPINMMTSRGCPFNCIFCVGSKMVGKKVRYFETH
                     RVVDEFEQLAAMKFKQINVVDDLFTSNKSRCIEVCNEIKRRGIDHPWTAFARVDTVNE
                     ELLTCMRDAGCTMLCFGIESGNQEILDKVKKKITLEKCKKAVETCRKVGISPMTSYIL
                     GLPGETEETMKKTIQFAKELSDNYGFHVLAPFPGTEVREMKDYYGIEILTDDWDLYDA
                     NRA"
BASE COUNT          932 a          464 c          555 g          946 t
ORIGIN      
        1 ggatatgtat gtgtgtttgt taacaatggg gctgtaaaaa tcaactttta gacagtttcg
       61 ccgggaccac gttaataatg ctgttttggt ttttaaagga agatttatta ttgatgagca
      121 tatagttgtg aaaataaaat gatataaagg aactatcgaa caaaccatga tactatcaat
      181 gatgactgct taatcaattg ttacactggt aatatattcc ttatcatcaa tggttataaa
      241 aaatgaaaaa ggttttgtta tcgatagttt tttgaatctg tcgccctgaa ccagcagcag
      301 gcatgcatcg tgaagcagtt cgcatatttt gatgattgtc aacgtttgag ccatttggaa
      361 agctgttgtt tccacaattt cttcatctga agcaacgtca aataaaacat cagatatctc
      421 atcaatgtcg caacctccca gttcatgaaa aatagccttg ccaaattgta cctcgttgta
      481 cagcgggata ttacattcag gtagcagtat attcgatggt tttgtgtatg atttgattat
      541 actatatgaa tcatatgctg aggcaaacac tattgaagga ataaatgaat ccgcattgct
      601 gtcaattttt aacgctatag actgaattgg attattgttt tgttttgatg aatataatgt
      661 aacagtattg ccaagttcat tggtaatcac aaacattata tcctgatcat ctattgttaa
      721 aagattgctt aaaaaatgtt ttactctttg catctcggga tgttgcagaa gctgttcata
      781 tttttcttca aatatttttt taaaaggatt ttgtgtcata ctgttcatga taacctcaat
      841 aaatatggaa atttttttta atctttcaat tatgacatta gtatggcatg gatgcttata
      901 atttaattca taccatttat attgctacat gcttgccaga tataccatca agccatttat
      961 ttataatgta tcataattac attttcactt ttcataaatg agtttagcaa gccatccact
     1021 gttaattcca aaacgggaaa ttatctacaa ataataataa atgtacaccc cgccgccgga
     1081 ggtggcgggg gtccacttcg tttcaacttg aaattatttt taaggaataa atattgcaag
     1141 caattaatac atagcagcta ttatagcaaa taaaatttca aataaaattt caaataaaat
     1201 tcatgtttat tttattagtg ttctataaaa aagttgatgc aaaatcaagg tgctcaaata
     1261 taagcttatg aaccgtgccc ccatgctctg tgtaatgcaa taacgatttc attgggttat
     1321 cattaatata gccttaataa actcaatgac ttcaagaaga tgcaatggat gcagttagta
     1381 aacatcacgg taaaaaagga atccatgctt ccctgcagga tagaagcaac ggtgcattcc
     1441 attatcacct tcacctgtta tttccggatt atgtataata cacaataact atcgccaaac
     1501 cctttagcgt tattctttaa tctgtttatt acaagaataa ttgaaaaatc aagaacttat
     1561 taatagttta atgcatacaa tagcggggat gacataattt atatgatgag cacaaaaata
     1621 tattgccaaa aattgtttgc ttatttaatg tccatagaat atatgataac ctatcataag
     1681 aatattaatt ttaattgata aaaggaagat catgaaagtt ttgcttgtca atccaccata
     1741 cccctttgaa gaagcaccat cacctccgtt tggattaatg gcccttgcag cataccttga
     1801 agcacagggc attgaagtaa agatagaaga ttatattgta aacagattta cgccagagcg
     1861 tgtttctgat gttttgtcgc agtttgcccc ggatgtagtt ggttcaacag gcgttaccat
     1921 gaatatcaaa aaagctcttt ccatagtaaa agtatataaa gagttagata aggatatagt
     1981 gacagttctg ggtggacctc atgtaacctt tgatgcgcat gccattcttc gcaattatcc
     2041 atttgtcgat gtgattgtga gaggtgaggg tgaagccacc ttccacgaat ttttgcaaca
     2101 tttacattca ataagcgctt atggggctat taagggaatt tcttatcggg ataaggataa
     2161 tgatattgtt cataatccaa tacgggaatt tattcctgat atcaatatac tccctttccc
     2221 agcacggcat ctggttgagc tatccaaata tcgcgcgtta gggtttccaa taaatatgat
     2281 gacctcccgt ggttgcccat ttaactgtat tttttgtgtt ggcagcaaga tggttgggaa
     2341 aaaggttcgt tattttgaaa cacatcgtgt tgtggatgaa tttgagcagt tagcagcaat
     2401 gaaatttaag cagattaatg ttgtggatga tctttttaca tccaataaaa gtcgttgtat
     2461 tgaggtttgc aacgagatta aaaggcgcgg gattgatcac ccatggactg catttgcccg
     2521 ggttgatact gttaatgaag agctgttaac ctgcatgagg gatgctggct gtaccatgct
     2581 ttgttttggc attgaaagtg ggaatcagga aattctggat aaagttaaga aaaagattac
     2641 actggaaaag tgtaaaaaag cggttgagac ttgccgaaaa gtgggaatta gtcccatgac
     2701 atcatacatc ctgggtttgc ctggtgaaac agaagaaacc atgaaaaaga cgatacagtt
     2761 tgctaaggaa ttgagcgata attatggttt ccatgttctg gctccatttc caggcactga
     2821 ggttcgtgag atgaaggatt attatgggat tgaaatttta acggatgatt gggacttata
     2881 tgatgcaaac agagcag
//
LOCUS       DAOOJH010000193         2835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_27346_length_2835_cov_8.063309, whole genome shotgun sequence.
ACCESSION   DAOOJH010000193 DAOOJH010000000
VERSION     DAOOJH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27346_length_2835_cov_8.063309"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1007)
                     /gene="hisG"
                     /locus_tag="PLJ75_12895"
     CDS             complement(114..1007)
                     /gene="hisG"
                     /locus_tag="PLJ75_12895"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012749648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPD06153.1"
                     /translation="MSLIKLGIPKGSLENATIELFKHAGFTISVSSRNYFPSIDDDEI
                     SCSLVRAQEMSKYVESGVLDVGLTGLDWILENESDVEVISDLMYSKVSARKARWVLAV
                     PNDSSIKTVEDCAGKTISTELVNFTRKYFSERNIPVKVEFSWGATEAKVVEGLVDAIV
                     EVTETGSTIKAHGLKIIYTLLETNTKLIANKKSMANPQKRAKIKQIALLLKGALNASN
                     LVGIKMNVPEEKLDVVIKLIPSLTSPTVSKLYNATWFSVESVINESVVRDLIPRLINA
                     GADGIIEYPLNKVVSQEDVCL"
     gene            complement(1004..1387)
                     /gene="hisI"
                     /locus_tag="PLJ75_12900"
     CDS             complement(1004..1387)
                     /gene="hisI"
                     /locus_tag="PLJ75_12900"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPD06154.1"
                     /translation="MIQLDFTKLDGLVPAVAQDYKTGEILMVAFMNEEAFELTLKTGI
                     VHYWSRSRKTLWKKGESSGNVQQVKEIRIDCDNDCVLLKINQIGDAACHTGYRSCFYR
                     VVEGDGLTIDGIKIFNPEDKYGDKK"
     gene            1694..>2835
                     /locus_tag="PLJ75_12905"
     CDS             1694..>2835
                     /locus_tag="PLJ75_12905"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD06155.1"
                     /translation="MDYSKTVNLPAGDFPMKANLPVREPHILQRWEKDNIYQLILKSR
                     ERNELYILHDGPPYANGHIHLGHALNKILKDIIIKHKTMTGFQAPYVPGWDCHGLPIE
                     LQISKELGDKAKTLPKQQIRKLCREYAQKFIDIQRDEFKRLGVFGDYQNPYLTMSSDY
                     EATIVEIFASLFELGFITKGKKPIYWCPTCVTALAEAEVEYHQHSSPSIFVKFKVDPS
                     SVSFKGVDANNLYVVVWTTTPWTLPANLAVCFHPDFDYSAYKSNNEYFIMADGLAESF
                     TSITGRQLGEKTPLTGEMIRLLKVYHPFIDRESKVILGNFVTLEQGTGIVHIAPGHGL
                     EDYIAGLEYGLDVFCPVDDEGKFTDEFAPMKGINVFDANPKIIELL"
BASE COUNT          862 a          591 c          499 g          883 t
ORIGIN      
        1 ctatagaaac ggggtagagg caatgttgca gcacatgact taacaaactg cctgccctta
       61 cgtatgaatt cctgctccat gaaattcctg taacattgag atgtcgcagg ttattacaaa
      121 caaacatcct cctgcgatac taccttgttg agcggatatt caatgatccc atcagcgccg
      181 gcattaataa gcctggggat aaggtcccgg accaccgatt catttatcac gctttcaact
      241 gaaaaccatg ttgcattata tagtttagat accgttggag acgtaaggct tggaataagc
      301 tttataacaa catcaagctt ctcttcgggt acattcattt taataccaac aagattgctg
      361 gcattgagtg caccttttag caatagagca atttgtttta tctttgcgcg tttttgcgga
      421 ttagccatac tttttttatt ggcaataagc ttggtattgg tttccagcaa tgtatagatg
      481 atttttagcc cgtgtgcctt aattgttgaa ccggtttcgg taacctcaac tattgcatca
      541 acaagccctt caactacctt tgcttcagtt gcaccccatg aaaattcaac tttcacagga
      601 atattccgct cgctgaaata tttccgtgta aaattgacaa gctcagttga tattgttttg
      661 cctgcacagt cctctactgt ctttattgat gaatcattgg gaactgcaag cacccatctg
      721 gctttccttg cgctcacctt tgagtacatc aggtccgaaa ttacctccac gtctgactca
      781 ttttcaagta tccagtcaag accggtcaat cctacatcaa gaaccccgct ttcaacatat
      841 tttgacatct cctgcgcacg aacaagcgaa caggaaatct catcatcatc gatacttggg
      901 aaataattac gcgatgatac tgatatggta aatcctgcat gtttgaaaag ttcaatggta
      961 gcattctcca gacttccttt aggaatacct aatttgataa ggctcatttt ttatctccat
     1021 atttatcttc aggattaaat atctttatgc catcaatggt aagcccatcc ccttccacaa
     1081 cacggtaaaa acagctacgg tatcctgtat ggcatgctgc atcaccaatc tgattaattt
     1141 ttaataatac acaatcgttg tcacagtcaa tacgaatttc ttttacctgc tgtacattgc
     1201 ccgaggattc cccttttttc catagcgttt tgcgggatct actccagtaa tgaacaatac
     1261 ctgttttaag tgtcaactca aatgcctctt cattcataaa ggcaaccatc aaaatttcac
     1321 ccgttttata atcttgagct actgctggaa ccaatccatc cagtttggta aaatctaatt
     1381 gtatcattag tatatcctga atatataaaa ttttttcaca ataactatcg ccaaaaaaaa
     1441 cgttaatggt tactaaacta ctgacacacc agtagtaaaa ctatttaata tctgctaata
     1501 ccataaaaac atttttaaca atatccataa aattaatata tacaaaaaac agaagttatg
     1561 tgatagtgta tcttatatca caggctgtta taaaaatcat taaaatgtat ttataaattg
     1621 ctaaaattct attgacaaat gtatttttgc actatcactg aatttcacat tactactgtg
     1681 ttgcgaggta tcaatggatt attctaaaac tgttaatctt ccagcaggtg atttccctat
     1741 gaaggctaat cttccggtac gggaacctca cattctacaa cgctgggaaa aagataatat
     1801 ctatcaatta attctaaaat cccgcgaaag gaatgaactg tatattttgc atgatggtcc
     1861 accgtatgca aacggacata tacaccttgg gcatgcatta aataaaattc ttaaagatat
     1921 catcattaaa cataaaacaa tgaccggttt tcaagccccg tatgtccccg gctgggactg
     1981 tcatggtctt cctattgagc tacagatttc aaaagaatta ggcgacaaag caaaaacatt
     2041 gccgaaacag cagatacgaa aactttgccg ggaatatgca caaaaattta ttgatattca
     2101 gcgtgatgaa tttaagcgat tgggcgtctt tggtgactac cagaatcctt atttaaccat
     2161 gtcatcagat tatgaggcaa ctattgttga gatatttgca tcgctctttg agcttggatt
     2221 tatcaccaaa ggtaaaaagc ctatctactg gtgccctacc tgcgttaccg ctttagctga
     2281 agctgaggta gaatatcacc agcacagctc accttcaatc tttgttaagt ttaaggttga
     2341 tccatcatcc gtttcgttta agggagttga tgcaaataat ctttacgttg tagtatggac
     2401 aacgacacca tggacactgc ccgctaattt agcagtgtgt ttccatcctg attttgatta
     2461 ttcagcctat aagagcaata atgaatattt tattatggct gatggacttg ctgaaagctt
     2521 taccagtata acaggcaggc aacttggtga aaaaacacct cttaccggtg aaatgatccg
     2581 cctgttaaaa gtatatcatc cttttattga cagggaatca aaagtgatat tgggcaactt
     2641 tgtaactctg gaacagggta ccggcattgt gcatattgca ccaggtcatg ggcttgagga
     2701 ttacatcgca gggcttgaat atgggctgga cgtattctgc cctgtggatg atgagggtaa
     2761 atttaccgat gaatttgcac caatgaaggg tatcaatgtc tttgatgcta acccaaaaat
     2821 aattgaactt ttaca
//
LOCUS       DAOOJH010000194         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_27736_length_2807_cov_8.863372, whole genome shotgun sequence.
ACCESSION   DAOOJH010000194 DAOOJH010000000
VERSION     DAOOJH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27736_length_2807_cov_8.863372"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLJ75_12910"
     CDS             complement(<1..248)
                     /locus_tag="PLJ75_12910"
                     /inference="COORDINATES: protein motif:HMM:NF013874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06156.1"
                     /translation="MKIFKIITLFPDFFNSPLKSGLLGKAIEKGLCHIECINLRQWGE
                     GQYRQCDDYPYGGGSGMVLMPVPLLKAIESCKGDSKVL"
     gene            complement(245..760)
                     /gene="rimM"
                     /locus_tag="PLJ75_12915"
     CDS             complement(245..760)
                     /gene="rimM"
                     /locus_tag="PLJ75_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPD06157.1"
                     /translation="MSSKEFFRIGVISGIRGFDGCLKVFIITDFPDRFSGSSPVYVNI
                     HGHYKEFTIIDFTIAAKHTGYCYLEGIHDDVSAQALKGCDIFIDADDVQLYDDNSFHY
                     SVIIGCVVYYNNENWGTVVDIVKTGAADVLVIKAHIGREYLIPFVESMVSTQRLNEHI
                     IEIFPVEGLLS"
     gene            complement(757..993)
                     /locus_tag="PLJ75_12920"
     CDS             complement(757..993)
                     /locus_tag="PLJ75_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPD06158.1"
                     /translation="MNYKELVEYMVKSLVDHPEQVEIKEIEGEKSTILELKVTKEDIG
                     KVIGKHGRIARAIRTILNASATKTGKRIVLEILD"
     gene            complement(1023..1202)
                     /gene="rpsP"
                     /locus_tag="PLJ75_12925"
     CDS             complement(1023..1202)
                     /gene="rpsP"
                     /locus_tag="PLJ75_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPD06159.1"
                     /translation="MDSRKRRDGAVIESLGHYQPIVKGDQFVVDRDKVTEWLKKGAQP
                     TETIAKLLKKAGITG"
     assembly_gap    1204..1303
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1304..1972)
                     /gene="rpe"
                     /locus_tag="PLJ75_12930"
     CDS             complement(<1304..1972)
                     /gene="rpe"
                     /locus_tag="PLJ75_12930"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPD06160.1"
                     /translation="MTDTVLLSPSIIATDLSLLGSTVSSFDSTSIHFLHIDVMDGNFV
                     PNVTIGPGYIQNLKHHTSIPLDIHLMIDKPELSLIHYSECNPYFLTIHMESTRQPIRL
                     LQQIKQAGIKAGLSINPSTPVAMLFDMVEYADMILIMSVEPGFYGQKFIPASLKRIEQ
                     LHNFINANNMDVVIEVDGGINEDTIADVVKAGARIIVAGSGVFNAHDINKQAKVLIQK
                     ACKAL"
     gene            complement(1965..>2807)
                     /locus_tag="PLJ75_12935"
     CDS             complement(1965..>2807)
                     /locus_tag="PLJ75_12935"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPD06161.1"
                     /translation="YSDVHNSLIRKGLKPHLKFYDVHDIDNDIILRQYPEPGEIVTEN
                     STIRLLVSRSNLTIDMPSVVGMELPFALNKLKNLHLYDKTISLRVGVISYIPSEKTTD
                     NVVIDQSPKSGQKIPLDKKVNLLVSSGSATQMVMPRVVGQSIDLCYDLLMSKKVVVQY
                     AIVPANDITQSGIIASVTPPEGQPLTQGQVVTLHIYYYAMKDKPYYAYERLQYTVPND
                     EGEGLYEVYVSDNKSKRLCFSQQLKPGNHIDCVFNRTGNARVYIMCNKKQVKVLSFDV
                     EEFD"
BASE COUNT          790 a          591 c          486 g          840 t
ORIGIN      
        1 tagagcacct tgctgtcacc tttacatgat tcaatggctt tcaataaagg gacgggcatc
       61 agcaccatgc cgctgccgcc gccgtagggg taatcatcgc attgcctgta ctgcccttca
      121 ccccattgtc gcaggttgat gcattcaata tggcataaac ctttttcaat tgctttccct
      181 aacagtcctg atttcagagg gctgttaaaa aaatcgggga atagcgttat aattttaaag
      241 atcttcacga aagcaaccct tcaacaggga aaatctcaat aatatgctcg ttaagacgct
      301 gtgtgctcac catactctca acaaaaggga taaggtattc cctgccaatg tgcgctttta
      361 ttaccagcac atcagcagca cccgtcttca caatatcaac aaccgtcccc cagttttcat
      421 tgttataata aacaacacac cctattataa cagaataatg aaaactattg tcatcataca
      481 actggacgtc atcagcgtct ataaaaatat cacatccctt tagcgcctgt gcggatacat
      541 catcatgtat tccttctaaa taacagtagc ctgtatgctt tgctgcaata gtaaaatcaa
      601 ttatagtaaa ttctttatag tgcccatgta tattaacata taccggtgaa ctgccagaaa
      661 aacgatcggg aaaatcagtg attatgaaaa ctttaaggca tccatcaaaa ccacggatgc
      721 cggagataac accaatacgg aaaaattctt tactgctcaa tcaagtattt ccagaactat
      781 ccttttacct gtttttgtag ccgaagcatt taatatggtt ctaatagcac gagcaatcct
      841 tccatgcttg ccaataacct tgccaatatc ttccttggtt acctttaatt caaggatagt
      901 agatttttca ccttcaattt ccttgatttc cacctgttca ggatgatcaa caagcgattt
      961 taccatatac tcaacaagct ccttgtaatt catcgttact gcctccacat atcaaaatcg
     1021 tattagcctg taattccggc ttttttcaat aattttgcaa tagtttccgt tggttgagcg
     1081 ccttttttaa gccactcagt aactttgtct ctatcaacca caaattgatc acctttcact
     1141 attggttgat aatgtccaag actttcaatg acagcaccat cacgacgctt tcgtgaatca
     1201 atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaagggct ttacaggctt
     1321 tttgtataag aacctttgcc tgtttattga tatcatgtgc attaaaaacg ccactgccgg
     1381 caacgataat tcgggcacct gctttaacaa cgtctgctat ggtgtcctca ttgataccgc
     1441 catcaacctc aatgactaca tccatattgt tagcattaat aaaattatga agctgttcaa
     1501 tcctttttaa tgaagcggga ataaacttct gtccgtaaaa tcccggctca accgacataa
     1561 tcaatatcat atcagcatat tctaccatgt caaacaacat tgcaacagga gtagagggat
     1621 tgatacttaa accggctttt attccagcct gttttatctg ttgcagcaat cgtattggtt
     1681 gacgggtact ttccatgtgt attgttaaaa aatagggatt acactcacta tagtgtataa
     1741 gcgatagttc tggtttatca atcatcaggt gtatatcaag tggtatgctg gtatggtgct
     1801 tcaggttttg aatgtagccc ggaccgatgg tgacattggg aacaaaatta ccatccataa
     1861 cgtcaatatg caaaaaatgt atagaagtgc tgtcaaacga tgatacagta gaacccagca
     1921 aagaaagatc ggtggcaatg attgatggcg acagtaatac ggtatcagtc aaattcttcg
     1981 acatcaaagc taagtacctt tacctgtttt ttattacaca taatatatac ccgtgcattg
     2041 ccggtacggt tgaatacgca atcaatgtgg ttacccggct tcaactgctg tgaaaagcac
     2101 aggcgttttg atttattatc gcttacgtaa acctcgtata aaccttcacc ttcatcattg
     2161 gggacggtgt attgcaggcg ttcataggca tagtacggtt tgtctttcat tgcataatag
     2221 tatatgtgca gtgttacaac ctgtccctgg gtaagcggtt gcccttcagg aggagttact
     2281 gatgcaataa taccactttg cgttatatca ttagcaggta ctatcgcata ctgcaccaca
     2341 accttttttg acatcaaaag atcataacac aaatcaattg attgcccaac tacccggggc
     2401 attaccatct gtgttgcgct gccgcttgaa actagcagat taaccttttt atccagtgga
     2461 atcttttgcc cggattttgg actttgatct atgacaacat tatcagttgt tttttctgat
     2521 gggatatagg atattacacc aactcgtagt gaaatggttt tatcatacag atgaagattt
     2581 tttagtttgt tgagagcaaa cggtaattcc atccccacaa cgcttggcat gtctattgtc
     2641 aggttgctgc gacttaccaa tagacgtatg gtgctatttt cggttactat ctcccctggt
     2701 tcaggatact gtcgtaaaat gatatcgtta tcaatatcat gaacatcata aaattttagg
     2761 tggggcttta atcccttacg gataagactg ttgtgcacat ctgaata
//
LOCUS       DAOOJH010000195         2724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_28974_length_2724_cov_11.861371, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000195 DAOOJH010000000
VERSION     DAOOJH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28974_length_2724_cov_11.861371"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="PLJ75_12940"
     CDS             <1..1572
                     /locus_tag="PLJ75_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPD06162.1"
                     /translation="LKAGFADDNKQFNYFVNFLQMYADKVTHYPMNISERIILYCKDA
                     GNKSVPNAKVKLYAGNKLLETGKTYADGSFMFYPSEYAANYKKYTITFEAFNTKAEIA
                     IDRQGKREHTVIMKAQRPQYKNVPLDILFVFDTTGSMGEEIARLKKTIEIINMNIASL
                     TAKPAVRFGMVLYKDKGDEYVTKVIPLTSNLNEFQDELAKVFAGGGGDGPEDLQSALY
                     DTVKKVKWNNNGIRLAFVITDAEPHLDYGQQYTYVNAAHDAKRDAIKIYSIGTGGLPL
                     MGELVLRQISQYTHARYIFLTYGESGESEGGKEGSVSHHTGANFQTDKLETIIIRFAK
                     EELAYLTDQPLEEGEAYFDAKKVEDETNEQTLQKLFDMAIAQLIDYSAINLQKGEPTS
                     VLPIKSSDETLATNAEYFTEQLSVALGKNKVFTQVERKNMQQILHEIGLGQTGIVDEA
                     SAAKAGKLLGAKMLVTGTLYQKKDSYELFLKLIRVESAEVLSATKAIIDKQLGVAEGI
                     KKVKPKQPVKKKAAP"
     gene            complement(1576..>2724)
                     /locus_tag="PLJ75_12945"
     CDS             complement(1576..>2724)
                     /locus_tag="PLJ75_12945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HPD06163.1"
                     /translation="FSYNLPSGYACEIISKSALENPLAHPQSNDSLCDIIKSNLNQFD
                     VELFYKEPDIRWHRIHFRAGTIRDRLIMQNIINHTGTIPEYESLQTVIDHNPEVLFTT
                     PSYVTIELCGRCNLDCIFCYRKQLSPNHGDMDFSTVQTLLEKMRIFNTPYAVCFGGSG
                     EPLLHPQFFKILELCSNEELIHTIVIETNGIYADTAFATTVATLNKKIITIVNINGYN
                     PETYQKLHNADHFATVYANIMHLHEMLKANNKNNSLYVQIMKINETEPFLDSYYDLFE
                     QHKIPIILQKQNTYTGRITDRRYSDLTPLQRKPCWHLQQDLYIVYDGSVAFCMQDFDC
                     SARRGTILHDDLKALWEQQKQHFVNDYKQNRATKPDCNSCDEWYTFNL"
BASE COUNT          841 a          480 c          612 g          791 t
ORIGIN      
        1 ttaaaagcag gatttgctga tgataataag cagtttaatt attttgtaaa ctttttgcag
       61 atgtatgctg ataaggttac ccattatccc atgaacattt ctgaaaggat aatactctat
      121 tgtaaggatg cagggaataa atccgtacca aatgctaagg ttaagctcta tgccggtaat
      181 aaacttctgg aaacagggaa aacctatgct gatggatcat ttatgtttta tccatcagaa
      241 tatgcggcaa actataaaaa atataccatt acctttgaag cgttcaatac aaaggctgaa
      301 attgccattg atagacaggg aaaacgagag cacactgtta taatgaaagc acagcggccg
      361 cagtacaaaa atgttcctct tgatatcctt tttgtatttg atacaacagg aagcatggga
      421 gaggagattg cccgtttaaa aaagactatc gaaattatca atatgaatat tgcctcatta
      481 acagcaaagc ctgctgtaag gtttgggatg gtgctctata aagacaaggg tgatgagtat
      541 gtcacaaaag ttatcccact taccagtaat ttaaatgaat ttcaggatga gcttgctaag
      601 gtttttgccg gcggaggcgg tgatggcccg gaggatttgc aaagcgctct ttatgatacc
      661 gtaaaaaaag taaaatggaa taacaatggg atacgccttg cttttgttat caccgatgct
      721 gaacctcatt tagattatgg gcaacaatat acctatgtta atgctgctca tgatgctaaa
      781 agggatgcta taaagatata ttccataggt accggcgggc ttcccctgat gggtgaattg
      841 gtattacggc agatttccca gtatactcat gcgcggtata tatttttaac ctatggtgaa
      901 agcggggaaa gtgagggagg taaagaaggt agtgtaagcc atcacaccgg tgctaatttc
      961 cagacggata agctggaaac aatcatcata cgatttgcta aagaagagct ggcatatctt
     1021 accgatcagc ctcttgagga aggtgaagca tattttgatg caaaaaaggt tgaggatgaa
     1081 accaatgagc aaaccctgca aaaacttttt gatatggcga tagctcaatt aattgattat
     1141 tcagccataa accttcaaaa gggtgaacca acatcggtat tgcccattaa atcatctgat
     1201 gaaacacttg caaccaatgc tgaatatttt accgagcagt tgtccgtagc actgggtaaa
     1261 aataaggtgt ttacgcaggt tgagcgtaaa aatatgcagc agatattaca tgagattggg
     1321 cttggacaaa ctggcattgt agacgaggca tctgccgcaa aagccgggaa gcttctgggt
     1381 gcaaagatgc ttgttaccgg tacactgtac cagaaaaaag acagctatga actgttcttg
     1441 aaacttataa gggtggaaag cgccgaggtt ttatctgcca cgaaagctat cattgataag
     1501 caactgggtg ttgctgaagg aataaaaaag gtaaaaccaa aacagccggt aaagaaaaaa
     1561 gcagcaccgt aatgtttaca gattaaaggt ataccattcg tcacaactgt tgcaatcagg
     1621 tttggtagct ctgttttgtt tatagtcatt gacaaagtgc tgtttttgct gttcccatag
     1681 tgctttgaga tcatcatgca gtatggtgcc gcgtctggca gagcagtcaa aatcctgcat
     1741 gcaaaatgct acgcttccat catatacaat ataaaggtcc tgctgcaggt gccagcatgg
     1801 cttccgctga agcggcgtta aatccgaata gcgcctgtcg gtaatacgtc ctgtgtaggt
     1861 attttgtttt tgtaaaataa tgggaatttt atgctgttca aataaatcgt agtaactatc
     1921 taaaaatggc tctgtttcat taatcttcat aatctgaaca tacaggctgt tgtttttatt
     1981 gttagccttt aacatttcat gcagatgcat tatattggca tatacggtgg caaaatgatc
     2041 agcattgtga agcttttgat aggtttcagg attatagcca ttgatattga cgatggttat
     2101 tattttttta ttcagtgtcg ctactgttgt ggcaaatgcg gtatcagcgt aaatcccatt
     2161 tgtttcgatg actatggtat gaataagttc ttcattactg caaagctcaa ggattttaaa
     2221 aaattgcgga tggagcaaag gctcgcctga accgccaaaa cacactgcat acggagtgtt
     2281 aaaaatgcgc atcttctcaa gtagtgtttg caccgtgcta aagtccatat caccatggtt
     2341 gggcgatagt tgttttcgat aacagaatat gcaatcaaga ttacatctgc cgcataattc
     2401 aatggtaaca taggatggcg tggtaaatag cacctcgggg ttatgatcaa tgactgtctg
     2461 gagcgattca tactccggta ttgtgccggt atgattgatg atattctgca tgatgagcct
     2521 atcgcgtata gtgccggctc taaaatgtat ccggtgccag cgtatgtcag gctctttata
     2581 aaaaagttca acatcaaact gattgagatt gctttttatt atatcacaca gtgaatcgtt
     2641 tgactgtgga tgtgctaatg ggttttcaag tgcagacttt gaaatgatct cgcatgcgta
     2701 gcctgacgga aggttatacg aaaa
//
LOCUS       DAOOJH010000196         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_29017_length_2720_cov_9.247280, whole genome shotgun sequence.
ACCESSION   DAOOJH010000196 DAOOJH010000000
VERSION     DAOOJH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29017_length_2720_cov_9.247280"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..797
                     /locus_tag="PLJ75_12950"
     CDS             57..797
                     /locus_tag="PLJ75_12950"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPD06164.1"
                     /translation="MKLFNTLLVVTVALVFTASSAFAAITVVSVKGDALYSDGGAYKP
                     LKKGQQLKEGTRIQTGTKSQAVLDIDGHMVTIKALTLIKVYKNIATQDASTNSIGLKY
                     GSINAKVKKLPKVKTSFLITTPVATSSVRGTEQNTSNGPLFGMQVFVPEGQLNVMSNG
                     ENRNVRGGLGYNKKEGDPRSDNVSNPMQNSFIGRLNPNNLTDEEMDMLNLYGDQLVDS
                     TQTPADILDNTEGNTMGNTTVDMNLIYP"
     gene            967..1398
                     /locus_tag="PLJ75_12955"
     CDS             967..1398
                     /locus_tag="PLJ75_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06165.1"
                     /translation="MRIISITIMCLFVATAGFSQSAIVAVNISQKTQATVEDAVKLFM
                     FSTVGSITVYEKDAPMLLEKGILPRLYDPNTPVTMGIVAYMMAKQANIKHSLMFNIFG
                     GQRYAVFACIAAGYLPSKAGEQYKLSGVELIEIMGRFGGDE"
     gene            1395..>2720
                     /locus_tag="PLJ75_12960"
     CDS             1395..>2720
                     /locus_tag="PLJ75_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HPD06166.1"
                     /translation="MKKILLLFLAIVTVHCGTLLFAQEEYIPLPDVAADPDLAVAEKP
                     LQPFTLNYGGWITPVVIDQRTSDTDLMASVTIARIWLTTTLWSGASLYVRVKDSYLAV
                     IREDDVDIDNTSNVFDLDIAAVTIPVIKNNLRLVLGRKYYVTGAGLVLNGRGDGGEVQ
                     LYTRFGDFKAFGMYSGLLNKDDNPYGLSSRDIADGAKRIFASAQYSKDFLYNNTLYMY
                     GLIEIDRQDEDDVKSDYDAQYIGAGVKGVISDKLTYYAEYVYQMGTSYATMSGVEEEN
                     DIKAMAVLAGLRYYWNVAYKPFINLQYAYGSGDKDATSYSDATGNTLNDDNRFMYFGT
                     FIGGFALRPQLGNLHVIRPGLGFSPFYKFGDRIKAMNLVFYYSYYMKDEKESPIGGGE
                     ADLPESFVGQAVDVMLRWPIYYDLSLFANYGLFLPGDAYPSGTPERHFIM"
BASE COUNT          799 a          527 c          640 g          754 t
ORIGIN      
        1 acaattatca ttttctttac atatttaatt tattataatc attgaggagg aaaccgatga
       61 agcttttcaa tacattactt gttgttaccg tagcgcttgt gtttacagca agctctgcat
      121 ttgctgccat tacggtggta agcgttaaag gcgatgccct gtacagcgat ggtggcgcat
      181 acaaaccgct gaaaaagggg cagcagctta aggaaggtac gcgcatacaa acaggcacaa
      241 aatcgcaggc tgtgcttgat attgacggac atatggtaac cataaaggca ctgacgctta
      301 taaaagtata taaaaatatt gccacacagg atgcatcaac aaacagtatt ggattaaaat
      361 atggcagtat caatgcaaaa gtaaagaaac ttccaaaggt aaaaacaagc tttttaatta
      421 ccacgccggt tgcaacatcg tcggtgcggg gcaccgaaca gaataccagt aatggtccac
      481 tttttggtat gcaggtattt gtccccgagg gacagctcaa tgtaatgtcc aatggcgaaa
      541 atagaaatgt aagaggcggt ttaggatata acaagaaaga aggtgacccg cgttcagata
      601 atgtaagcaa tcccatgcag aatagtttta ttgggaggtt gaatccaaac aatcttaccg
      661 atgaagaaat ggatatgctt aatctatatg gcgatcagct ggtcgactcc acccagacgc
      721 cagccgatat actggataac accgagggga atacaatggg gaatacaacg gtagatatga
      781 atttaatata tccataataa taacctcacc cttaatccct ctccttataa aggagaggga
      841 aacatcccgg ggcgagggca cccctcttct tactaaggag aaggaaatat ttccgggatg
      901 agtttgcccc cttgccttat tacgaagggg ggcagggggt gcggttatac caacaaagga
      961 ggtcttatgc gaattatctc tattacaata atgtgtttgt ttgttgcaac tgctggattt
     1021 tctcagagcg ctatcgttgc agtcaatata tcacaaaaaa cacaggcaac agttgaagat
     1081 gcagtaaagc tgtttatgtt ctcaacggta ggaagcataa ccgtttatga aaaggatgct
     1141 cccatgctcc ttgaaaaggg aatcctccca cgcctctatg atcccaatac gccggtcaca
     1201 atgggtattg ttgcgtacat gatggcaaag caggcaaata taaagcactc gcttatgttc
     1261 aatatatttg gcggtcagcg ctatgcagtt tttgcctgta ttgcagcagg gtacctgccc
     1321 tcaaaggctg gcgaacagta taaactcagc ggtgttgaac tcatagagat catgggcagg
     1381 tttggaggtg atgaatgaaa aaaatactac tattattttt ggcgatagtt actgttcatt
     1441 gcggcaccct cctttttgca caggaagaat atattcctct gcccgatgtt gctgctgacc
     1501 ctgatttagc agttgccgaa aaaccactgc aaccatttac cttaaattat ggaggatgga
     1561 ttactcctgt ggttattgac cagcgtacat cagatactga tttgatggcg tctgttacca
     1621 ttgcaaggat atggttaacc actaccttat ggtcaggtgc cagcctctat gttcgtgtta
     1681 aggacagcta tcttgcagtt atacgtgagg atgatgttga tattgacaac acgagcaatg
     1741 tatttgatct ggacatcgct gcagtaacca tccctgttat aaaaaataac ctgcggctgg
     1801 tactgggaag aaaatattat gttaccggtg cagggcttgt gttaaatggt cgcggcgatg
     1861 gcggcgaggt gcagctgtac accagatttg gcgactttaa agcatttggc atgtacagcg
     1921 ggctactcaa taaggatgac aacccctatg ggttaagctc acgtgatatt gccgatggag
     1981 caaagcgcat ctttgcatcg gcacagtatt ccaaagattt tttgtataac aatacattgt
     2041 atatgtacgg acttatagag atagacaggc aggatgagga tgatgttaaa tctgattatg
     2101 acgcacaata tattggtgca ggcgttaagg gtgtaataag cgataagctg acatattacg
     2161 ctgaatatgt atatcagatg ggaacatcgt atgctacaat gtcaggtgtg gaggaagaaa
     2221 acgatattaa agcaatggca gtattagcag gtttgcgcta ttactggaat gttgcctaca
     2281 agccttttat caatttacag tatgcctatg gttcgggtga taaggatgcc acaagctaca
     2341 gcgatgcaac cggtaataca ctcaatgatg acaatcgatt tatgtacttt ggtacattta
     2401 taggtggttt tgcactgcgt ccacagctgg ggaatttaca tgttatccgc cccggtctgg
     2461 gattttctcc attctataag tttggggacc ggataaaggc tatgaacctt gttttttact
     2521 attcatacta catgaaggat gaaaaagaat cgcctatcgg tggtggtgag gcagatttgc
     2581 cggaaagctt tgtaggacag gctgttgatg ttatgctgcg ctggcccata tattatgacc
     2641 tttcattatt tgctaattat ggattatttt tacccggtga tgcctatcca tcgggcacac
     2701 cagaacgcca tttcatcatg
//
LOCUS       DAOOJH010000197         2697 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_29379_length_2697_cov_11.910295, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000197 DAOOJH010000000
VERSION     DAOOJH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29379_length_2697_cov_11.910295"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1046
                     /locus_tag="PLJ75_12965"
     CDS             39..1046
                     /locus_tag="PLJ75_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006194906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HPD06167.1"
                     /translation="MNILVTGSAGFIGYYTALKFLQQGYTVIGVDAINSYYDVSLKEA
                     RNALLLNDKNYIFYQCDIANYEELSHIFKKHTPDYVVHLAAQAGVRYSLDHPFEYIQS
                     NIVGFMNIVECCRHNPVNHLVYASSSSVYGLNSCVPFSVHDTVDHPVSLYAATKKSDE
                     LIAHCYSHLYGIPTSGLRFFTVYGPWGRPDMAYFTFTKSIVEDKPIDVYNYGNMERDF
                     TYIDDIVEGVYRITFTIPQGKPHFDTMHPDPASSTAPYRIYNIGNNKPVSLLYFIEVL
                     EKEIGKKATIHFLPMQPGDVLATFADIDELTQATGYSPVTSIEQGLSLFVQWYKKFYT
                     V"
     gene            complement(1043..1567)
                     /locus_tag="PLJ75_12970"
     CDS             complement(1043..1567)
                     /locus_tag="PLJ75_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06168.1"
                     /translation="MVNKRFVIVFTVVLLSVQPLHSHVATSSIIEENRDFWNQLIQTI
                     TIANKNLLYASYVAYKDKLYDLSIDSFQECIAQNPDNQLIQGVANYYIGKNFYHIGEY
                     KKAALFFLATERYSMNQYSSLKAAALINAALCYIAIDDIALAKTYLQKSLQYDIEGTY
                     RTTADSLLQSIKEH"
     gene            complement(1576..1746)
                     /locus_tag="PLJ75_12975"
     CDS             complement(1576..1746)
                     /locus_tag="PLJ75_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06169.1"
                     /translation="MGSIKQPEIQQEQQHPETIVSQWNNFKLLESQQIYFSVGKATIK
                     HILIKREPLIGA"
     gene            1956..2651
                     /locus_tag="PLJ75_12980"
     CDS             1956..2651
                     /locus_tag="PLJ75_12980"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPD06170.1"
                     /translation="MSIAKKKNFIILAIVLLIFVPCIMGYPYKFDFITVNDIVKQIKK
                     AFSDVDSYQASFVMISDRIGKRQTQSGTIWYKSPHYLRVDFDSPYGQRIVSDGKQVWI
                     YIPSLNIVAEQDLNSGTLFSVGTGPGLNRLFSKYHYRFDGKEQPREESNNRYYVLFLK
                     QKETRSGFRTLKLWVNEQYFITKAYGETASGKNITLEIKNVTTKIDVKKGFFTFDPPS
                     NARIIKNPMLAEE"
BASE COUNT          846 a          448 c          508 g          895 t
ORIGIN      
        1 atataaaaaa tattttttaa atattacggt gtgatgcaat gaatatactt gttacaggtt
       61 ctgctggttt tattggttat tatacagcgc tgaagttttt acaacagggc tatacggtta
      121 ttggtgttga tgctatcaat agctactacg atgtttcatt aaaggaagca cgaaatgcgc
      181 tgctgctcaa tgataaaaac tatatatttt atcaatgcga tatagctaat tatgaggaac
      241 tatcgcacat atttaaaaaa catacccctg actatgttgt tcaccttgct gcacaggcag
      301 gggtgcgcta tagccttgac catccctttg agtatataca gagcaatatc gttggcttta
      361 tgaatattgt ggaatgttgc cgacataatc ctgtgaatca tctagtttat gcatcgtcaa
      421 gttccgtata cggacttaat tcatgcgtgc cattttcagt acatgatact gttgatcatc
      481 ctgtatcgct gtatgctgct actaaaaaat cggatgaatt gattgctcac tgttattcgc
      541 atttatatgg aatccctacc agtggtctta gattttttac cgtctatggg ccatggggtc
      601 gtccggatat ggcatacttt acctttacaa aatctattgt tgaggataag cccatagatg
      661 tttataacta tggaaatatg gaacgggatt ttacctatat tgatgatatt gttgagggtg
      721 tttataggat aacgtttacc ataccgcagg gaaaacctca ttttgacacc atgcatcctg
      781 atccggcatc aagcacagcg ccgtaccgga tttataacat cggcaataac aagccggttt
      841 cactgctgta tttcattgag gtactggaaa aagaaatagg taaaaaagca acaatacatt
      901 ttttgcccat gcagccgggt gatgtgcttg caacgtttgc cgatatagat gagttaacac
      961 aagccactgg ttacagccct gtaacttcaa ttgaacaggg attgtcattg tttgttcaat
     1021 ggtataaaaa gttttatact gtttaatgtt cttttatgga ttgcaaaaga ctatcagcag
     1081 ttgttcggta ggtgccttca atatcatact gtaatgattt ttgcagatat gtttttgcta
     1141 acgctatatc atcaattgca atataacata aagcagcatt gatcaatgct gcagccttta
     1201 aggaggaata ctgattcatg ctgtatcgtt cggttgctaa aaaaaacagc gccgcttttt
     1261 tatattcacc aatgtgataa aaatttttac caatataata attggcaacg ccctgtatca
     1321 gttgattatc agggttttgc gcaatacatt cctgaaaaga atctatggac aggtcatata
     1381 acttatcttt ataggctaca taacttgcat acaataaatt tttattggcg atagttattg
     1441 tttgaataag ctgattccag aaatctctat tttcctcaat gatactgctg gttgcaacgt
     1501 gtgaatgtaa tggctgcact gacaataaca ctacagtgaa aactatgaca aaccgtttat
     1561 tgaccatagt aaatctcatg ctccaataag tggttctcgc tttattagta tatgctttat
     1621 ggtagctttc ccaacagaaa aataaatttg ctgtgattcc agcaatttaa aattattcca
     1681 ttgcgaaaca attgtttctg gatgttgctg ctcttgctgt atttcaggtt gttttatgga
     1741 acccatagtt tactccttat ggtagcatca cttaaatagt aaaggcatga taaaaatttg
     1801 tctacacaaa aaaattataa tttttacact cagtaatctt tttgcttttt tgtattgact
     1861 ttttaaaaaa gctttttagg ttgacatagt catatttgtt ataaaaaata tgttggctta
     1921 tccttatata tctaaaaagg cacttaaagg ctggtatgtc aatagcaaaa aagaaaaatt
     1981 ttataatttt ggcgatagtt cttttaattt ttgtaccctg cataatgggg tatccgtata
     2041 aatttgactt tatcacagtc aatgatattg ttaagcagat aaaaaaggca ttctctgatg
     2101 ttgatagtta tcaggcaagc tttgttatga tttctgaccg tattgggaag cgtcagactc
     2161 agagtggaac aatatggtat aaatctcctc attacctgcg tgttgatttt gattctccct
     2221 atggacagcg aattgtatct gatggaaaac aggtatggat atatatccct tcattgaaca
     2281 ttgttgcaga acaggattta aactcgggta cacttttttc agttggtacc ggacctggtt
     2341 taaacaggct cttttcaaag taccattacc ggtttgatgg gaaagaacag cctcgtgagg
     2401 aaagtaataa tagatattat gtattatttt taaaacaaaa agaaacacgg agcggattca
     2461 gaacattaaa actatgggtt aatgagcagt attttattac aaaggcatat ggtgaaacag
     2521 ccagcggaaa aaatataaca ctggaaataa aaaatgttac tacaaaaata gatgtaaaaa
     2581 aaggattttt tacgtttgat cctccttcaa atgcccgaat cattaaaaat cccatgttag
     2641 cagaggaata gcatatggta gcaggaattg gcgaaacatt acgcaatgct cgcgaag
//
LOCUS       DAOOJH010000198         2696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_29395_length_2696_cov_5.232109, whole genome shotgun sequence.
ACCESSION   DAOOJH010000198 DAOOJH010000000
VERSION     DAOOJH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29395_length_2696_cov_5.232109"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..765)
                     /locus_tag="PLJ75_12985"
     CDS             complement(127..765)
                     /locus_tag="PLJ75_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06171.1"
                     /translation="MNNMTQNPFKKIFEEKYEQLLQHPEMQKIKNFLSNLLSIDDQDF
                     MFMITNELGNTVTLYASKQNNNPIQSITLKLYSNADSFIPSIVFASAYDSYSIIKSYT
                     KPSNILLPECNIPLYNEVQFGKAIFHELGGCDIDEISDVLFDVASDEEMLETIAFQMA
                     QTLTIIKICELLHDACLLLVQGDRFKKLSITKPFSFFITIDDKEYITGVTID"
     gene            1511..>2696
                     /locus_tag="PLJ75_12990"
     CDS             1511..>2696
                     /locus_tag="PLJ75_12990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD06172.1"
                     /translation="MKVLLINPPYPFEEAPSPPFGLMALAAFLEAQGIEVKIEDYIVN
                     RFTPERASDVMSQFAPDVVGSTGVTMNIKKALSIVKVYKELDKDIVTVLGGPHVTFDA
                     HAILRNYPFVDVIVRGEGEATFHEFLQHLHSISAYGAIKGISYRDKDNDIVHNPIREF
                     IPDINILPFPARHLAELSKYRALGFPINMMTSRGCPFNCIFCVGSKMVGRKVRYFETH
                     RVVDEFEQLAAMKFKQINVVDDLFTSNKSRCIEVCNEIKRRGIDHPWTAFARVDTVNE
                     ELLTYMRDAGCTMLCFGIESGNQEILDKVKKKITLEKCQKAVEACRKVGISPMTSYIL
                     GLPGETEETMKKTIQFAKELSDNYGFHVLAPFPGTEVREMKDYYGIEILTDDWDLYDA
                     NRA"
BASE COUNT          879 a          414 c          518 g          885 t
ORIGIN      
        1 ggaccaggtt aatagtgctg tttgggtttt caaaggaaga tttattattg atgagcaaat
       61 acctgtgaaa ccacgatgat ataaaggaac tatcgcacaa atcatggtac tatcaatgat
      121 tactgattaa tcaattgtta caccggtaat atattcttta tcatcaatgg ttataaaaaa
      181 tgaaaaaggt tttgttatcg atagtttttt aaatctgtcg ccctgaacca gtagcaggca
      241 cgcatcgtga agcagttcac atattttgat tattgttaac gtttgagcca tttggaaagc
      301 tattgtttct agcatttctt catctgaagc aacgtcaaat aaaacatcag atatctcatc
      361 aatgtcgcaa cctcccagtt catgaaaaat agccttgcca aattgtacct cgttgtacag
      421 cgggatatta cattcaggta gcagtatatt cgatggtttt gtgtatgatt tgattatact
      481 atatgaatca tatgctgagg caaacactat tgaaggaata aaagaatctg cattgctgta
      541 tagttttaaa gttatagact gaattggatt attattttgt tttgatgcat ataatgtaac
      601 ggtattgcca agttcattgg taatcataaa cataaaatcc tgatcatcta ttgataaaag
      661 attgcttaaa aaatttttta ttttttgcat ctcgggatgt tgcaagagct gttcatattt
      721 ttcttcaaat atttttttaa aaggattttg tgtcatattg ttcatgataa cctctccata
      781 atttttttta aactcttaat tatgacatta gtgtggtata attgcttata atttaattca
      841 taacattgat agcacaacat gcttgccaga tattccatca agctatttat taaaaattta
      901 tcataattac attaacattt taataaatga gtttagcaag caattaataa atagtagcta
      961 ttatagcaaa taaaatttca aataaaattc atgtttattt tattagcgtt cgttataaaa
     1021 ggttgatgta aaagtaaggt gctcaaagat aagcgtatga accgcgctac ctgctttgtg
     1081 tcatgcagta atgatttcat tgtgttttca ttgatatagt cttaatataa tcagtgactt
     1141 ccagaagatg taataaattg agttattaaa catcactgtg ataaaggaaa ctatgcttca
     1201 atgcaggatc aaaacaacag tgcattccat tatcaccttc acctgtcatt ttcggattat
     1261 gtataataca caataactat cgcccaaccc tttgcaattt ttatttttaa aaaaaaataa
     1321 tacactgata attgaaaagc caggaattta ttgtttggta taatcataag tatgctgatg
     1381 tcataatttc tatgataagc acaaaaatat attgccaaaa tttgtttgct tatttaatgt
     1441 ccataaaata tgaaatgcta tagcataaac tataatgaga atatcaaatt gaaacgataa
     1501 aaggaagatc atgaaagtat tgcttatcaa tcccccatat ccctttgaag aggcgccatc
     1561 acctccgttt ggattaatgg cccttgccgc attccttgaa gcacagggca ttgaagtaaa
     1621 aatagaagat tatattgtaa acaggtttac accagagcgt gcttcagatg ttatgtcgca
     1681 gtttgcaccg gatgttgttg gttcaacagg cgttaccatg aatatcaaaa aagctctttc
     1741 catagtaaaa gtatataaag agttagataa ggatatagtg acagttctgg gtggacctca
     1801 tgtaaccttt gatgcgcatg ccattcttcg caattatcca tttgtcgatg tgattgtgag
     1861 aggtgagggt gaagccacct tccacgaatt tttgcaacat ttacattcaa taagcgctta
     1921 tggggctatt aagggaattt cttatcggga taaggataat gatattgttc ataatccaat
     1981 acgggaattt atacctgata tcaatatact ccctttcccg gcacggcatc tggctgagct
     2041 atccaaatat cgggcgttag ggttcccaat aaatatgatg acctcacgtg gatgtccgtt
     2101 taactgcatt ttttgtgttg gcagcaagat ggttggaaga aaggtgcgtt attttgaaac
     2161 acatcgtgtt gtggatgaat ttgaacagtt agcggcgatg aaatttaaac agataaatgt
     2221 tgtagatgat ctttttacat cgaataaaag ccgttgtatt gaggtttgca acgagattaa
     2281 aaggcgcggg attgatcacc catggactgc atttgcccgg gttgataccg ttaatgaaga
     2341 gcttttaacc tacatgaggg atgctggctg taccatgctt tgttttggaa ttgaaagtgg
     2401 aaatcaggaa attctggata aagtgaagaa aaagattaca ctggaaaagt gtcagaaagc
     2461 tgttgaggca tgccgaaaag tgggaattag cccaatgaca tcgtatatac tgggtttgcc
     2521 tggtgaaaca gaagaaacca tgaagaagac aatacagttt gctaaggaat tgagtgataa
     2581 ttatggtttt catgttctgg caccatttcc cggcaccgag gttcgtgaga tgaaggatta
     2641 ctatgggatt gaaattttaa cggatgattg ggacttatat gatgcaaaca gagcag
//
LOCUS       DAOOJH010000199         2650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_30078_length_2650_cov_11.699037, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000199 DAOOJH010000000
VERSION     DAOOJH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30078_length_2650_cov_11.699037"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1345)
                     /locus_tag="PLJ75_12995"
     CDS             complement(<1..>1345)
                     /locus_tag="PLJ75_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HPD06173.1"
                     /translation="SGDNKADNVLNDDIESFWCTNKSHNATAEYIVIDYGLAVAVNVI
                     ELFPSPSGKSTFPSDFRLESSVDGSNWKIIKTENRYSLDEDRYRVLLPMNAFQFLKLY
                     IIKPKRIAAKYFSEIGGIKVGIQGASAITASSCSSYEHDPSNLLMNDSAKYWESETSN
                     TPSRQWIEIDIGRDFPLAHIGLISTSSAELFPEQFSIESSGDGKIWTTIAELKRFKAE
                     ANRSYIFGSYQFNARYIRMLCTTVQSSKQNYCAQIAQICAYASLPSDSHSHVNAAVQY
                     ATIFQPGIVTLAGDGDTTPATVVQANDSRLRDASTIFKGIVQMAPDGSSEKGLVVQAS
                     DSRLSPATELKYGIVRLAYDREMVEGVAVQSIDSRLKEATENSFGIVKLCPDGEYVEN
                     SVVKGNDSRIQKATEKKFGIVRLADDGEDSEFSVVQGNDRRLKDATIVARGIVELA"
     assembly_gap    1346..1445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1446..2261
                     /locus_tag="PLJ75_13000"
     CDS             <1446..2261
                     /locus_tag="PLJ75_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06174.1"
                     /translation="ENKTKPFHSMKTIVFIGVHPGDVAIKQPYQLFKIQNASIIIPYM
                     LENNTRHYIFIPSDSYLDLIPCMVEYANDIASFISFVKKGIMPDFIIINSELSNDDSA
                     MIQSLCPSSHVEIAGQSVEPPNNDSKRAVDAGNIDINLLSNNPVFLARVHLRNLDLPR
                     VKQILLDMDLSESDAQYIIAFLNTMLQHSQKEELHKHLEKLNELKHAYEFYYYCIAHD
                     DHNVKQLIEKADKKDLVTFQTLIQKVKLLFPTKEENLRFVDFENYIWEKNNER"
     gene            2251..>2650
                     /locus_tag="PLJ75_13005"
     CDS             2251..>2650
                     /locus_tag="PLJ75_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPD06175.1"
                     /translation="MNVKKLLSHIFVLLFFSVSYAQQPIEVYFTNPDSNETVTPIQKA
                     LIHTINSASVSIYAALYELSDPLIIDALCNAKQRGIDVRLVLESDRLSSTVKTKLESF
                     AIPIRLDNRKGFMHNKFFIFDNKAVWTGSYN"
BASE COUNT          806 a          532 c          419 g          793 t
ORIGIN      
        1 gctaactcca ctatccctct ggcaactatc gttgcatctt taagccgcct gtcgttcccc
       61 tggaccacag aaaactcact gtcttcgcca tcatctgcaa gccttacaat cccaaatttt
      121 ttttcggtag ctttttgaat ccttgaatca tttcctttca ctactgaatt ttcaacatat
      181 tccccatcgg gacacagttt aacaattcca aagctatttt ctgtggcttc cttcaatcgg
      241 gaatcaattg actgaactgc aacaccttca accatttctc tgtcatacgc caagcgtaca
      301 attccgtatt taagctctgt tgcaggagaa agtcgtgaat cagaagcttg aacaaccaat
      361 cccttttcgc ttgaaccatc aggagccatc tggacaatgc ctttgaatat tgtacttgca
      421 tcgcgtaatc tgctatcatt tgcctgcacc actgttgcag gtgttgtatc gccatcgcct
      481 gctaatgtaa caatccctgg ctgaaaaata gttgcatatt gcactgctgc attaacatga
      541 gaatggctat cagaaggcaa tgaggcatat gcgcagatct gagcaatctg ggcacaatag
      601 ttttgtttag aagattgtac tgttgtgcaa agcatgcgta tatagcgtgc attaaactga
      661 tacgaaccaa agatatatga gcggttagct tcggctttaa atcgttttaa ttcagctatg
      721 gttgtccata tcttcccgtc cccacttgat tctattgaaa attgttcagg aaataattct
      781 gcactggatg ttgaaataag accaatgtga gcaagaggga aatcccttcc tatatcaatt
      841 tcaatccact gacgagatgg tgtattgctt gtttcagatt cccaatattt tgcagaatca
      901 ttcattaata aatttgatgg atcatgctca tatgatgaac atgaacttgc ggtaattgca
      961 cttgctccct gtataccaac ctttatcccc cctatctccg aaaaatattt tgcagctatt
     1021 cgctttggtt taatgatata cagtttcaaa aattggaatg cattcattgg cagcaataca
     1081 cgatacctat cttcatcaag cgaatatctg ttttctgttt ttattatctt ccagttgctg
     1141 ccatcaacac ttgattcaag tctaaaatct gatgggaatg ttgatttgcc tgacggagaa
     1201 ggaaacaact ctatgacatt gacagccaca gccagaccat aatcaataac aatatattcg
     1261 gctgtagcat tatgtgactt attggtacac cagaagcttt ctatatcgtc atttaaaaca
     1321 ttgtctgctt tattatcacc agatgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnngaaaa caaaacaaaa ccattccact ccatgaaaac aattgtattt attggtgtcc
     1501 accctggtga tgtagcaata aagcaaccat atcagctttt taagatacaa aacgcctcaa
     1561 taattattcc ttatatgtta gaaaataata cacggcacta catctttatt ccatccgata
     1621 gctatcttga tctaataccc tgtatggtag aatatgctaa tgatattgca tcattcatca
     1681 gttttgtaaa aaaggggatt atgccagact tcatcattat aaatagtgaa ttgagcaatg
     1741 acgatagtgc catgatacaa tctctttgtc catcgtctca tgttgaaatt gcaggtcaat
     1801 ctgttgaacc accaaataat gattcaaaac gtgctgtaga tgctggcaat attgatatta
     1861 atcttttatc aaataatcct gtatttttag cgcgtgtcca tttacgtaat ttagatttac
     1921 cccgggtaaa acagattcta cttgatatgg acctttcgga atctgatgca caatatatca
     1981 tcgcattttt aaatacaatg cttcagcatt cacaaaaaga agagttgcat aagcaccttg
     2041 aaaaacttaa tgaattaaaa catgcttatg aattttatta ttattgtata gctcatgatg
     2101 accataatgt aaaacaactc atagaaaaag ccgataaaaa agatcttgtc acattccaga
     2161 cattaataca aaaggtaaaa ttgctttttc caacaaagga agaaaattta cggtttgttg
     2221 atttcgaaaa ttacatctgg gaaaaaaaca atgaacgtta aaaaattact atcgcacata
     2281 tttgtattac tctttttttc cgtatcctat gcacagcagc caattgaagt atattttacc
     2341 aatcctgaca gcaatgaaac agtaacgcca atacaaaaag ccttgataca taccatcaac
     2401 agtgcttcgg tgtcaatcta tgctgcttta tacgaactat ccgatccact catcattgac
     2461 gcattatgca acgcaaaaca acgtggcatt gacgtcaggc ttgttctgga atctgacagg
     2521 ttgagcagta cagtaaaaac aaaactggaa tcttttgcaa tacccatacg gctggataac
     2581 cgcaaaggat ttatgcacaa caaattcttt atttttgaca acaaagcagt ctggacaggg
     2641 tcatacaata
//
LOCUS       DAOOJH010000200         2596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_30946_length_2596_cov_12.286895, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000200 DAOOJH010000000
VERSION     DAOOJH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30946_length_2596_cov_12.286895"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PLJ75_13010"
     CDS             <1..1221
                     /locus_tag="PLJ75_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06176.1"
                     /translation="QEKRNNIHAMQTIYRQIQSVSEEIETCMFNHETKNHKRARNLLI
                     INVMFAFGILFLFLKPLFNAIALNRWLLLAILIGIQVASAGYYIIYTMTHSINKLIAP
                     LQQQKEEYQIKLETLLNQNNILLDSITIDEIYEFLLQYFEEYNEYNEKQVDLLTIQME
                     IDEQKSLADLEREKKEIESNYAFIKSQMDSFIQDIDYGHIETVEEAIAAKADIQHHCD
                     EKKMMIQALQNKLKETPEEIADFNNEKLEVENEIHTLEKELYAIKEKKRVMTIVKDSF
                     NDAVERFYHDALTVLTHTVKTMYPELTGDQFPHIDEEYIKKALYTSFDSTAPASVKYL
                     LYVTVVFGLYTMLQQQLPRIQLPLIIDDPFVLMDEDAIYNFTEVMQQWAHSRQIIIFS
                     HNSNIKKMYDVTEL"
     gene            1224..2216
                     /locus_tag="PLJ75_13015"
     CDS             1224..2216
                     /locus_tag="PLJ75_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06177.1"
                     /translation="MDKNGQLTFSDDPLLQGTNEMYMFIESGDFNLAVKKLDELLTIN
                     PEYPGLAESYRVARFWDSRKKNLDELSKGKETADFLMKQWEEFEAFAMDKNVVHTTAY
                     QKVMRYVFFTASEHYITAFNDRENPTDNLKLLMQLGTCLVRIAEYQHTIEVLEYARSQ
                     YQSNACILSLLAEAYYHVNDIPRSLLYFREAFLLNPQEIDLPLLSSKPIIEAVELTKQ
                     TKPDCKDIREWIPIVAFIHDIFYVKRQLNSSQLEQLKKDILRLEENYQTLNKDRVEQS
                     NIIPRLINRYLWMMDYYEFQHYDQQSLLDIRSRLLAIDKELFEGYFQKKSFSKP"
     gene            2307..>2596
                     /locus_tag="PLJ75_13020"
     CDS             2307..>2596
                     /locus_tag="PLJ75_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD06178.1"
                     /translation="MELHASYNPKIAESTWYTVWEQEGLFHGDENDPRDAYSIVIPPP
                     NVTGNLHMGHALNNTLQDILIRWQRMKGKVTCWMPGMDHAGIATQNVVERLL"
BASE COUNT          906 a          436 c          494 g          760 t
ORIGIN      
        1 caggaaaaaa gaaacaatat tcatgcaatg caaactatat accggcagat acaatcggta
       61 tctgaagaaa ttgaaacctg catgtttaat catgaaacta aaaatcataa acgtgctcga
      121 aatcttttaa ttataaatgt aatgtttgca tttggtatat tatttttatt tctgaagcca
      181 ctgtttaatg caattgcatt gaacaggtgg ttattgttag ctatacttat agggattcag
      241 gttgcaagtg ctggctatta cattatttat accatgacac actctattaa taaattgatt
      301 gcacccttac aacaacagaa ggaagagtat caaataaaat tggaaacgct tctcaaccag
      361 aataatattc tacttgatag tattaccatt gatgaaattt atgaatttct tttacaatat
      421 tttgaagagt ataacgaata caatgaaaaa caggttgatt tattaaccat acagatggag
      481 atagacgagc aaaaatcatt agccgatctt gaacgggaaa agaaagaaat agaaagtaat
      541 tatgctttta taaaaagtca gatggattct tttattcagg atattgatta tgggcatatt
      601 gaaactgttg aagaagctat tgctgcaaaa gccgatattc aacatcattg tgatgaaaaa
      661 aagatgatga ttcaagcatt gcaaaataaa ttaaaagaaa cacctgaaga aattgctgat
      721 ttcaacaatg aaaaattgga agttgaaaat gaaatacaca ccctggaaaa ggagttatat
      781 gctatcaaag aaaaaaaacg agttatgaca atcgttaagg actcgtttaa tgatgctgtt
      841 gagcgttttt accatgatgc actaacagtg ttgacgcata ctgttaaaac aatgtatcca
      901 gaacttacag gggaccagtt tccacacatt gatgaagaat atataaaaaa agcgttatat
      961 acttctttcg atagtacagc gcctgcatcg gttaaatatt tgctctatgt cactgttgtg
     1021 tttggattat acaccatgtt gcagcagcaa ttaccccgga tacaattacc gctcattatt
     1081 gatgatcctt ttgttttaat ggatgaagat gcaatctata attttacaga agtaatgcaa
     1141 caatgggcac attcccggca gattatcatt ttttcccata attctaatat aaaaaaaatg
     1201 tatgacgtta ctgaattgta actatggata aaaacgggca attaacattt tcggatgatc
     1261 ctttactcca gggaaccaat gaaatgtata tgttcattga gtcaggggat tttaacctgg
     1321 cggtaaaaaa gctggatgag cttttaacaa taaatccgga atatcccgga cttgctgaat
     1381 catatcgtgt tgcacggttc tgggattcaa gaaaaaaaaa tctggatgag ttatcaaagg
     1441 gaaaggaaac agcagatttt ttaatgaaac agtgggaaga attcgaagca tttgccatgg
     1501 ataaaaatgt agttcataca acagcatatc aaaaggtcat gcgctatgtt ttttttactg
     1561 cttccgaaca ctatatcact gcattcaatg atcgggaaaa tccaactgat aacctgaaac
     1621 ttttaatgca attaggtacc tgtcttgtgc gtattgctga atatcagcat accattgagg
     1681 tgctggaata tgcacgctca cagtaccaga gtaatgcatg catattgtcg cttttagccg
     1741 aggcgtacta tcatgtaaac gatatcccgc gatcattgct ctatttcagg gaagcatttt
     1801 tacttaatcc acaggagatt gacttgccat tgctatcgtc aaaaccaatc atcgaggcag
     1861 ttgagttaac caaacaaaca aaacctgact gcaaggatat ccgcgaatgg ataccaattg
     1921 ttgcatttat acatgatata ttttatgtta agcggcagct caattcatca cagcttgaac
     1981 agctaaagaa ggatattttg agattagaag aaaactatca aaccttgaat aaggatagag
     2041 ttgagcagtc aaatattatc ccacgcttga ttaaccgcta cctgtggatg atggattatt
     2101 atgaatttca gcactatgat cagcaaagtt tgctggatat ccgcagtcgt ttgcttgcca
     2161 ttgataaaga gctttttgaa ggttatttcc agaaaaagtc attttcaaaa ccatgaaaat
     2221 gacaatctga aaaaattcat tgacactaca tatactgctg aataattaac taccagcaaa
     2281 acagaataga ttacaggtgg tggagtatgg aattacatgc atcgtataat ccaaagattg
     2341 ccgagtctac atggtataca gtgtgggaac aggaaggact ttttcacggt gatgaaaatg
     2401 atccccgcga tgcctattct atagtaatac cgccgcccaa tgtaaccggt aacctccaca
     2461 tgggacatgc attaaacaat acgttgcagg acatactcat tcgctggcaa aggatgaaag
     2521 ggaaagttac ctgctggatg ccgggtatgg accatgcagg gattgctaca cagaatgttg
     2581 ttgaacgcct tttatt
//
LOCUS       DAOOJH010000201         2575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_31331_length_2575_cov_5.377778, whole genome shotgun sequence.
ACCESSION   DAOOJH010000201 DAOOJH010000000
VERSION     DAOOJH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31331_length_2575_cov_5.377778"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2095)
                     /locus_tag="PLJ75_13025"
     CDS             complement(<1..2095)
                     /locus_tag="PLJ75_13025"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPD06179.1"
                     /translation="MAFSYANNDYNNTVKQNVRIVTSLYEPFVMFKDGKLVGFDIDLI
                     NEICKQNHLTYSITITSFQDIFTQIEKGTADMAIGCIYVTEEREEIFQFSKSYLEGGL
                     VLVVPANSDIKTIYELNNKKLGVKKDATGDLFASKIVQKGYSIEIIRYQDTVDSFQAL
                     VNGSVDAVLNDYLNSVYLLNKYFSGDIKIVRGRWSDIIYGRRGIAYPISSNNQHLYEI
                     INKSIHDLKSSNFIDMLYWKWFAIMPPPNIIRRVMIYTGLVAVSIGITCLLFLHFNKR
                     KKFKELSMIEEHFRNLINDLNVAVYIIQNNSIVLTNHEGAHIAGSSIESIIGYPLDYL
                     FSEYTVINDKELHYSSFTDLSNMVQAGPMVYRARWKRKDGTVLDVLFRIRHVQWMGNS
                     ALECIVNDTTDINRLLVEREKLKGQLLQSQKLEIVGQITGGIAHDFNNILTVILGYAN
                     LLEASVDAKNREAVTKIIEAGNHARDIVSKMLTFARKKVFLEQKMHLHHIIQNSILLL
                     KTALPKSCTVDMQLHATVDTIVGDTTQIQQMLMNLVINASEAMPEGGTINIETTNVTI
                     EHERIGKVETIHAGEYILLSIKDSGIGIAEKDIEHIFEPLFTTKEKGSGFGLSIVYGI
                     VKQHKGYIDVVSIPGKGSTFYIYLPVITENRVEASLKIIDNKKESLPALGGEIVIIDD
                     DANIGSLYKESLHLAG"
BASE COUNT          792 a          490 c          387 g          906 t
ORIGIN      
        1 aaccagccag atgcaagctt tccttatata aagaaccaat attagcatca tcatctatta
       61 tcacgatttc accacctaaa gctggtaatg attctttttt attgtcaata attttcaacg
      121 atgcttctac tctattttca gttatgactg gtaaatatat ataaaatgtg cttccttttc
      181 cggggattga tactacatca atatatcctt tatgctgttt aacaatacca taaactatcg
      241 acagaccaaa tccgcttcct ttttcttttg tagtaaataa aggctcaaag atatgctcaa
      301 tatccttttc agcaatacct atcccgctat ctttaataga tagtaaaata tactcacctg
      361 catggatggt ttcaaccttc ccaatacgct catgttctat ggtaacatta gttgtttcaa
      421 tattgattgt tcccccctcg ggcattgctt ctgaagcatt gatgacaaga ttcatcagca
      481 tttgctgtat ctgtgttgta tcgcctacta tggtatcaac ggttgcatgt aattgcatgt
      541 caactgtgca gcttttaggc aatgctgtct tcaataatag tatactattt tgaatgatat
      601 ggtgcaaatg catcttttgc tccagaaaaa cttttttccg tgcaaaggtt aacatttttg
      661 aaacaatatc cctggcatga ttaccagctt caatgatctt tgttaccgcc tctctatttt
      721 tggcatcaac actggcttca agcagattag catagccaag tatgacggtt aatatattgt
      781 taaagtcatg tgcaatgccg cctgttatct gacctactat ctccaatttt tgtgattgca
      841 acaactgtcc tttcagcttt tcgcgctcca ctaataatct gtttatatcg gtagtatcat
      901 tgacaatgca ttcaagagcg ctattgccca tccattgtac atggcgaatt ctaaaaagta
      961 catccagtac tgttccatct tttcttttcc atctggctct ataaaccatg ggacctgcct
     1021 gtaccatgtt gcttaaatct gtgaaagagg aataatgaag ttctttatca tttattactg
     1081 tatattccga aaagagataa tcaagtggat agcctatgat ggattcaatg gagcttcctg
     1141 caatatgtgc accttcatga ttggttaata ctatgctatt attttgaatt atataaacag
     1201 caacattaag atcattgatg aggttcctga aatgctcctc aatcatggat agctctttaa
     1261 atttttttct tttattaaaa tgaagaaata gtaaacatgt tataccaatg gatacggcaa
     1321 ccaatcctgt atagatcatc actctccgaa taatgtttgg aggcggcatg attgcaaacc
     1381 atttccagta tagcatgtct ataaaattac ttgatttcaa atcatggata cttttattga
     1441 ttatctcata aagatgctga ttgttgcttg atataggata cgctatcccc ctgcgaccat
     1501 agatgatgtc actccatcta cccctaacaa tttttatatc acctgaaaaa tatttattta
     1561 ataaatacac cgagtttaga taatcattga gcactgcatc aacactgccg ttaaccagtg
     1621 cttgaaatga atcaactgta tcctgatatc gtataatttc aatggaatac cctttttgta
     1681 ctattttact ggcaaacaaa tcacccgttg catctttttt tacccctaac tttttattgt
     1741 ttaattcata tatggtttta atatcactat tcgctggcac aaccagcacc aaccctccct
     1801 caagatagct tttggaaaat tgaaatatct cttcacgctc ttctgtaaca tagatacagc
     1861 caatagccat atcagccgtg cctttttcaa tctgagtaaa tatatcctga aatgatgtta
     1921 tggttatgga ataggttaag tgattctgct tgcaaatttc attgattaaa tctatatcaa
     1981 atccaaccag tttaccgtct ttaaacataa caaatggctc gtacagcgaa gtaacaatgc
     2041 gtacattctg ttttacggta ttgttgtaat cattattggc ataggaaaat gccatggcat
     2101 tgccaggatt cattaacagt aatacacaac aatatttgag caagtgtttc atgagtattt
     2161 tttacaacgc cattatcaaa atgtcaatgg tatttatgct ataatgcaat attcaatgct
     2221 accatatttt ttaattcttc tctatacata tcatacaatt aaaaaaaagg gattccttta
     2281 cggaatcccg agaaaattta cattaaaaac gtgcgacaat tcgcagcttt aatgggggct
     2341 ggagtaacag ttaatagtgt tacatcttgt aaccaacatg aatacctaga atatggcttt
     2401 tttaagttat tttttattaa atttttttta caaaggaaaa attttacttg attatttagt
     2461 attttagcat atatttaatt acatgattgt ttttaattcc tttttaatag gaggaaaatt
     2521 atgaaatcaa atacctactg catagataac agatgctcat actggaggaa tactg
//
LOCUS       DAOOJH010000202         2550 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_31781_length_2550_cov_6.740281, whole genome shotgun sequence.
ACCESSION   DAOOJH010000202 DAOOJH010000000
VERSION     DAOOJH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31781_length_2550_cov_6.740281"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1574
                     /locus_tag="PLJ75_13030"
     CDS             237..1574
                     /locus_tag="PLJ75_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPD06180.1"
                     /translation="MIFLNEIRAWVWGAPLIILLLGTGIYLTILLKGLQFRTLFSSLY
                     LALIKRKEYDAHPGDISHFQALMTALSATVGTGNIAGVASAIAIGGPGALFWMWLTGL
                     FGMATKYSEAVLAVKYREVDALGTMSGGPMYYIEKGLKNKWLAFIFALFAACAAFGTG
                     NIIQSNSVAEAMHATFNVPSWVTGIFLLMITYLVIMGGIKSIAKAASAIVPFMILFYI
                     IVGTVILILFYKNIPHAFTLIFVHAFTPVAAAGGFAGAGVKMAMQMGVSRGLLSNESG
                     LGSSPIAAAAAKTDEPVRQALVSMTQTFIDTIIVCTFTGLVILVTGAWETGKTAAALT
                     EHAFSMGLPGTWGGIVVSISLIFFAYSTILGWSYYGEKAIEYIAGERAIKPYRLLWVI
                     IVFIGAVGRLDVVWAVTDIMNGLMAFPNLIALLGLSFVIKNETNHFFSKHTTI"
     gene            1617..>2550
                     /locus_tag="PLJ75_13035"
     CDS             1617..>2550
                     /locus_tag="PLJ75_13035"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPD06181.1"
                     /translation="MNYRQNKKYYLKVVLTQFYSVPYYMVMVALLFCFSCSRFEWVRD
                     PYTLYVHLSAEPGHLNPITSTEAIASSINTYIYETLLDRDYDTLELKPLLASKWEISS
                     DKLHYRFYLKKNIRWSDGKPFTADDIVYSFHVIKDPKVANAPLKVYYIDVVSVKKISP
                     YIVEFTYSRPYFLALEICGSIPVVPKHIFDDGTDFNTHKNNRHPIGTGPYTFSKWETG
                     KRIELEINPLYWGKKPDIKKVVYKVIPEPSVALQMLKKGEIDVMSLRPIQWVRQTDSQ
                     HFKEAFYKFMYYQPYYSYIGWNAKNPLFNNKNVRK"
BASE COUNT          760 a          437 c          532 g          821 t
ORIGIN      
        1 gttttccagt gtccagatat ctgaaatttc ttttcgcgaa taacgatcaa tcatatattt
       61 cccatgctca ttttataatt aaaatgaaaa agttaattct ctttggccat taaaattaat
      121 caagtaaaaa aaggtatatt ttgttaatca tcaagaatta ctgcttgacg gatatattat
      181 tgataaagca acaataacat tgtgaatgta ctaaattata agataaaggt ttaagtatga
      241 tttttttgaa tgaaattaga gcatgggtct ggggagcgcc tctcattatt ttattgctgg
      301 gtactggtat ttatttaaca atactgctta aagggttgca gtttagaact ttattttcat
      361 cattatatct ggcattgata aaaagaaaag aatatgatgc gcaccccggc gatatatcac
      421 attttcaggc tctcatgact gcactatcag caaccgttgg aaccggcaat atcgctggtg
      481 tggcttcggc aattgctatt ggcggccctg gtgcattgtt ctggatgtgg cttaccggtt
      541 tatttgggat ggcaacaaag tacagtgaag cagtgcttgc ggttaagtat cgtgaggttg
      601 atgcattggg aaccatgtca ggtggtccca tgtattatat tgaaaaagga ttaaagaaca
      661 aatggcttgc ttttattttt gcactgtttg ctgcctgtgc agcgtttgga accggtaaca
      721 taatacagtc caattcggta gctgaggcaa tgcatgctac atttaatgta ccatcatggg
      781 taacaggaat atttttatta atgataacct atcttgttat catgggcggt ataaaaagta
      841 tagcaaaggc agcttcggcg atagttccct ttatgatact gttttatatc attgttggca
      901 cagtcatact gatactattt tataaaaata taccccatgc atttacatta atctttgtgc
      961 atgcatttac tccagtggca gcggctggcg gttttgcagg tgcgggtgtt aaaatggcta
     1021 tgcagatggg tgtttcacga ggattgcttt ccaatgaatc aggtttagga agttcaccga
     1081 tagctgctgc tgcagcaaaa accgatgaac ccgtacgaca ggcactagtt tccatgacac
     1141 agacttttat tgatactata atagtttgta cttttacagg gctggttatt cttgttaccg
     1201 gtgcgtggga aacgggaaaa actgccgctg cgttaaccga gcatgcattt agcatggggc
     1261 tacccggcac atggggaggt atcgttgttt cgatatctct gatatttttt gcatattcaa
     1321 ctatcttagg atggtcatat tacggtgaaa aagctataga atatatagca ggagaaagag
     1381 caataaagcc ttatcgttta ctgtgggtaa ttattgtatt tattggtgct gttggcaggc
     1441 tggatgttgt gtgggcagtt accgatatta tgaacggttt aatggctttt cctaatctca
     1501 tagcgttgtt ggggttgtcg tttgttataa aaaatgaaac aaatcatttc tttagcaaac
     1561 atactacaat ataaccatgc atactgtaca tagattgtaa ccaaaagttt gataccatga
     1621 attatcgcca aaataaaaaa tattatttaa aagtggtgct aacacaattt tacagcgtac
     1681 cctattatat ggtgatggta gcattgctgt tttgtttttc atgtagccgt tttgaatggg
     1741 tgcgcgatcc atatacgctc tacgttcatc tttctgctga gcctggacac ctcaatccta
     1801 taacatcgac agaggcaatt gcatcatcaa taaataccta catttatgaa acactgctgg
     1861 acagggatta tgatacgtta gagttaaaac cgctattagc ttcaaaatgg gagatatcat
     1921 ctgataaact acactaccgc ttttatttaa agaagaatat acgctggagt gatggtaagc
     1981 cttttaccgc cgatgatatt gtctattcgt ttcatgtcat caaagatcca aaggttgcca
     2041 atgctccttt aaaggtgtat tatattgatg tagtcagtgt taaaaaaatt tcaccataca
     2101 ttgttgagtt tacttattca cggccatatt ttttagcact ggagatatgc ggcagtatac
     2161 ctgttgttcc taagcatatc tttgatgatg gcaccgattt caacacgcat aaaaataaca
     2221 gacatcccat tggtaccgga ccatatacat ttagcaaatg ggaaacgggg aagcgtattg
     2281 aattagagat taatcccctt tactggggta aaaagcctga tattaagaag gttgtttata
     2341 aagtaattcc cgaacccagc gttgcattgc agatgctaaa aaagggtgag atagatgtca
     2401 tgtcgctgag gcccattcaa tgggtacggc agactgattc acaacatttt aaggaggcat
     2461 tttataaatt catgtattac cagccctact atagttatat tggctggaat gcaaaaaatc
     2521 ccctgtttaa caataaaaat gttcgcaaag
//
LOCUS       DAOOJH010000203         2543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_31924_length_2543_cov_9.457797, whole genome shotgun sequence.
ACCESSION   DAOOJH010000203 DAOOJH010000000
VERSION     DAOOJH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31924_length_2543_cov_9.457797"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLJ75_13040"
     CDS             <1..341
                     /locus_tag="PLJ75_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metal-dependent phosphohydrolase"
                     /protein_id="HPD06182.1"
                     /translation="ASGDIIQKSDFQYPGPKPHTRETAVVMLADAIEAAARSIQEPTI
                     EKLESLIKRIINNRLNEGDLDHADITMNELNKIQHSILRVLQGIFHSRIEYPTDDEIN
                     RLEQRSEHGH"
     gene            331..777
                     /gene="ybeY"
                     /locus_tag="PLJ75_13045"
     CDS             331..777
                     /gene="ybeY"
                     /locus_tag="PLJ75_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPD06183.1"
                     /translation="MAINITIESEDISVSKYLKTFCKKNIKAILRYLHIDNVAVTLVL
                     TGDASIARFNRNYRKKKGPTDVLSFAYREAPTPLADGLPEYLGDVIISLETAQKQAQE
                     YNVTLHDEMLRLIVHGMLHLLGYDHEQYYKKKQMQKKEEELLQHLR"
     gene            complement(796..>2543)
                     /locus_tag="PLJ75_13050"
     CDS             complement(796..>2543)
                     /locus_tag="PLJ75_13050"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPD06184.1"
                     /translation="MDNEIAKRIQQWQQPPFDNETISEINQLVQQNNEKELYERFYTN
                     LEFGTGGLRGIIGAGTNRMNIYTVGMATQGLANYILQKGSAEQGVVIAYDSRRMSDVF
                     SKEAASILAANGIMVYLFSDITPTPICSYAIRYFKATAGIVITASHNPPEYNGYKVYW
                     NDGGQVVPPQDKEIIDEVQKINAITQIKKMDFHKAAGNKLIQLAGNEIINSYADKLSG
                     VIFKSQELSPINIVFTPLHGTGYNIVPMILQKFGFTNVNVVEKQAIPDGNFPTVASPN
                     PEEPEALTMAIALAKTMNADIVLATDPDADRMGVAFKDTNGEYTLINGNQIGSMLLYY
                     ILLQRQHHNRLPRNSFVVKTIVTTELQQAIADYFHVSMENVLTGFKWIAKKIRDYEHT
                     HTFIFGGEESYGYLPLDFVRDKDAVSSCYFFAEMTHWLHKQNKTLNDFLYEIYSRFGY
                     YNEKLVSLTLKGVEGIEKIKTIMKHFRSNPPQQFAGIKVVSILDGKAGIVDIASGATK
                     PANLPPSDVLQYSLENRSKVTMRPSGTEPKIKFYFSSCKQADDIKQASQEVNHCNDQL
                     ADELLNTIKTIVNID"
BASE COUNT          721 a          520 c          515 g          787 t
ORIGIN      
        1 gtgccagtgg agatattatc cagaaaagtg attttcagta tcccggtcct aaaccgcata
       61 cacgggaaac tgcagttgtt atgcttgcag atgcaattga ggctgctgca cgttctattc
      121 aggaaccaac catcgaaaaa ttagaatcgt tgataaaacg aataatcaat aaccgtttga
      181 acgaaggtga tctggatcat gctgatatta ccatgaatga gcttaataag attcagcatt
      241 ccatactgcg ggtattgcag ggaatatttc actcacgcat tgaatatcca acagatgatg
      301 aaattaatag attagagcaa cgatcagagc atggccatta acattactat tgaaagtgaa
      361 gatatttcag tttctaaata tttaaaaaca ttctgtaaaa aaaacatcaa ggctatacta
      421 cggtatctcc acattgataa tgttgctgta acgcttgtgc ttaccggtga tgcatcaatt
      481 gcccgtttta acagaaacta tcgcaaaaaa aaaggaccaa ctgatgtgct ttcctttgcc
      541 taccgcgagg cgccaacgcc acttgctgac ggtttgcctg aatatcttgg tgatgtaatc
      601 atatcacttg aaacagcaca aaaacaggca caagaatata atgttaccct gcatgacgaa
      661 atgctgcggc ttattgttca tggtatgttg catctgttgg gctatgacca tgagcaatat
      721 tacaaaaaaa aacagatgca aaaaaaagaa gaagaactgc tgcagcattt acgttgataa
      781 gcaacaaaaa taattttagt ctatattcac aatagttttt atggtgttca gcagctcatc
      841 ggctaattgg tcgttgcaat gatttacctc ctgtgatgcc tgcttgatat catcagcttg
      901 cttgcatgag ctaaaataaa atttaatctt gggctccgta cctgatggac gcatggtaac
      961 ctttgatcta ttttctagac tatattgcaa aacatccgat ggtgggagat tggccggttt
     1021 agtggctccg cttgcaatgt ctacaattcc tgctttgccg tcaaggatgg atacaacctt
     1081 tataccagca aactgctgtg gagggttgct gcgaaaatgt ttcatgatgg tttttatttt
     1141 ttctattcct tccactcctt tcaaggtcag tgaaaccaat ttttcattgt agtacccaaa
     1201 cctgctataa atttcatata gaaagtcgtt aagcgttttg ttttgtttat gcagccagtg
     1261 tgtcatctct gcaaagaaat agcatgagct tacggcatct ttgtcgcgca caaaatcaag
     1321 gggtaaataa ccatagctct cttcaccgcc aaagataaag gtatgcgtat gctcatagtc
     1381 ccgtatcttc tttgcaatcc acttaaaacc ggtaagaaca ttttccattg atacatggaa
     1441 gtaatcagct atagcctgct gcagttcggt ggtaacaatg gtcttaacca caaaactgtt
     1501 gcggggcaat ctattgtgat gctgccgctg taataaaata taatacagca gcatgctccc
     1561 tatctgattg ccgtttatta atgtatactc gccattggta tctttgaatg caacacccat
     1621 acggtcggca tcagggtcgg tggcaagtac tatatcggca ttcatggttt tagccaatgc
     1681 tattgccatg gtaagcgctt caggttcttc gggatttggt gatgcaaccg ttgggaagtt
     1741 gccatccgga attgcctgct tttccacaac gttgacattg gtaaatccaa atttttgtaa
     1801 aatcatgggt acaatgttgt agcctgttcc atgaagcggc gtaaaaacaa tattgatggg
     1861 cgatagttcc tgtgatttga atataacacc ggaaagttta tctgcatagc tattgattat
     1921 ttcattgcca gccagttgta tcaatttatt tccagcagcc ttgtgaaaat ccatcttttt
     1981 aatttgcgta attgcattaa ttttttgcac ctcatcaatg atctctttat cctgcggagg
     2041 aacaacctga ccgccgtcgt tccagtatac tttatagccg ttatattcgg gagggttgtg
     2101 gcttgcggtt ataacaatac cggcggttgc cttgaaatag cgtatggcat acgagcagat
     2161 gggtgttggc gtaatatcgc tgaatagata taccatgatg ccgttagcag ctaaaatact
     2221 ggctgcctcc tttgaaaaaa catccgacat cctccgtgag tcataggcta tgacaacacc
     2281 ctgttcagcg ctcccttttt gtaaaatgta attggcaagt ccctgcgttg ccataccaac
     2341 ggtatagata ttcatcctgt tggtaccggc tccaattata ccccgtaacc ctcctgtacc
     2401 aaattctaaa ttggtataga agcgttcata cagttctttt tcattgtttt gttgtaccag
     2461 ttgattaatt tcactgatgg tttcattatc aaacggtggc tgttgccatt gctgtatgcg
     2521 ctttgcaatt tcgttgtcca taa
//
LOCUS       DAOOJH010000204         2436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_33891_length_2436_cov_10.454851, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000204 DAOOJH010000000
VERSION     DAOOJH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33891_length_2436_cov_10.454851"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLJ75_13055"
     CDS             <1..712
                     /locus_tag="PLJ75_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPD06185.1"
                     /translation="ITTDDFSSGWGLTGWAQQANLGGFPALTDPTNFNYSNEYLAYAN
                     HKAYKSFSLSGYTNAILLFEAYIDIEGGYDFFSVNYKIGTGDPFVTGQQLLRISGSFS
                     NPWYHDYYPFPFDISSYTGTNCSIGFRLQSDGSYQYTGVAIRNFAIVNANTSVTNAYE
                     VMDGTSMASPYVAGLAAMLIAFNPDYTYQDVANALKNGGETVASLAGITTTGKSINAW
                     GSLKYINTPAGLSAQQIQ"
     gene            727..1080
                     /locus_tag="PLJ75_13060"
     CDS             727..1080
                     /locus_tag="PLJ75_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06186.1"
                     /translation="MKKIYCILLITGMMFCAHACAHVNQPDTKVTPVKQQQKFAQNVV
                     PCEYLFTVDETCDVQCLYSLLVKDYSITNIKAIGNNLFLVTFTIDPGMDALLSMYNNI
                     STIKAIQPNFKYKLQ"
     gene            complement(1093..>2436)
                     /locus_tag="PLJ75_13065"
     CDS             complement(1093..>2436)
                     /locus_tag="PLJ75_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD06187.1"
                     /translation="RPVRLTKDCLICHGDPAQSKALWGRDDGKDPTGGTMEGWKAGEV
                     HGAFEIVYSLKEYVAARFQILIISVVANLIILGLAIVLIRRVVRKGLSPLDEMVKSLE
                     EMNRGEGDLTHKIAIESDDEVGKLALLFNQFIDNLRLLILAVKDAADHVASSSTEMTQ
                     SSQQLANVAQDQAASIEETSSAMEEIKATIDSVSENAKHQAKKADATRNSMEYLADAI
                     NRINHNAQEAYTMADETHDYAKDGESILGSTVSGMKEINESSKRITEIVTIISDISDQ
                     INLLSLNASIEAARAGEHGKGFAVVAEEISKLADQTAESSKEINKLIHETNNKVNAGS
                     GLVERTAESLRKIIENVKTTAVLMENIAQSSQELNRMSNDVKGDVEEVNKMSEEISMM
                     MEEQSASTNEIIKAINQINEITQVVSSGSEELAAASEELSSQAESLNQVVKKFNT"
BASE COUNT          659 a          537 c          444 g          796 t
ORIGIN      
        1 tatcaccaca gatgattttt ccagtggatg gggattaaca gggtgggcac agcaggctaa
       61 tcttggcggc tttccagcac ttactgatcc aactaatttc aattattcca atgaatactt
      121 agcatatgca aatcacaagg catataaaag ttttagttta tcaggctata ctaatgcgat
      181 cctgttattt gaagcgtata ttgatattga aggaggttat gattttttca gtgtcaatta
      241 caaaattggt acgggagatc cttttgtaac cgggcaacaa ttattgagaa tatcagggag
      301 ttttagtaat ccatggtatc atgattatta tccattccca tttgatattt catcgtatac
      361 cggtacaaac tgctccatag gattccgatt acaatctgat ggaagttatc aatacactgg
      421 tgttgcaatc cgtaattttg caatagtaaa tgcaaatacc tccgtcacca acgcatatga
      481 ggttatggat ggcacatcta tggcttcccc gtatgttgca gggcttgccg ctatgcttat
      541 tgcctttaat ccggactata cctatcagga tgtagccaat gcactaaaaa atggagggga
      601 aacagttgcc tctctagctg gtataacaac aacaggcaag tctatcaatg catggggttc
      661 attgaaatat atcaatactc cagcagggtt gtctgcacaa caaattcagt aataggtgtg
      721 tagtgcatga aaaaaatata ttgtatactg ttaatcacag gcatgatgtt ttgtgctcat
      781 gcctgtgcac atgtaaatca acctgataca aaggtaactc cggtgaaaca acaacaaaag
      841 tttgctcaga atgttgttcc ctgtgaatat ttatttactg ttgatgaaac ctgtgatgtg
      901 cagtgcttat actcgctact agtaaaagac tacagcatta ccaacattaa agccataggt
      961 aacaatctat tcctggttac ttttactatt gatcctggca tggacgcttt attgtccatg
     1021 tataataata tatctacaat aaaggctata cagccaaatt ttaagtataa actacagtaa
     1081 tattcatctt ccctatgtat taaatttttt tacaacctga ttcaaacttt ctgcctgtga
     1141 ggaaagctct tcagatgctg cagccagttc ctcagaaccg cttgacacca cctgggtgat
     1201 ttcattgatc tggttgatag ctttgataat ctcattggtt gaagcactct gctcttccat
     1261 catcattgat atttcttcag acatcttgtt aacttcctct acatcgccct tgacatcatt
     1321 gctcattcgg ttgagctcct gtgatgactg cgcaatattt tccataagca ctgctgttgt
     1381 ttttacattt tcaataatct ttcgcagtga ttcggccgtc ctttccacaa gccctgaacc
     1441 tgcgtttact ttattatttg tttcatggat aagcttattg atctcttttg aactttcggc
     1501 agtttgatca gccaattttg atatctcttc tgctacaacg gcaaagcctt tcccatgctc
     1561 acccgctcgt gcagcttcta ttgaagcgtt gagtgaaagc aaattgatct ggtcagaaat
     1621 atctgaaatt attgttacaa tttcagtaat acgttttgag ctttcgttta tctctttcat
     1681 gccgcttaca gtactcccta aaattgattc gccatccttt gcatagtcat gggtttcgtc
     1741 tgccattgta tacgcttcct gggcattgtg attaatacga ttgatagcat cagctaaata
     1801 ttccatagag tttcgggttg cgtctgcttt ctttgcctga tgtttggcat tttctgaaac
     1861 cgagtcgata gttgccttaa tctcttccat ggcagaggat gtttcttcaa tggaagctgc
     1921 ctgatcctga gcgacattgg caagctgctg gcttgactga gtcatctctg ttgatgacga
     1981 agccacatga tctgctgcat ctttcacagc caggataagc aagcgcagat tatcaataaa
     2041 ttgattaaac agcaatgcaa gttttccaac ctcatcatcg ctctcaatag ctattttatg
     2101 tgttaaatcg ccttcacccc tgttcatctc ttccagcgat ttcaccattt catccagtgg
     2161 actcaatcct ttccttacaa cccttcgtat aagaactatc gccaaaccta aaatgattaa
     2221 attggcgaca acacttataa taagaatctg gaatcgtgcg gcaacatatt cctttaacga
     2281 atagacaatc tcaaacgctc catgaacctc tcctgctttc cacccttcca tggtgccgcc
     2341 cgttgggtct ttaccatcgt cgcgccccca taatgctttt gattgtgcag ggtcgccatg
     2401 gcatatcaaa caatcctttg taagccttac cggcct
//
LOCUS       DAOOJH010000205         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_35586_length_2354_cov_8.745977, whole genome shotgun sequence.
ACCESSION   DAOOJH010000205 DAOOJH010000000
VERSION     DAOOJH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35586_length_2354_cov_8.745977"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PLJ75_13070"
     CDS             <1..650
                     /locus_tag="PLJ75_13070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD06188.1"
                     /translation="WYQCDVSDYTQVKKTFTQALRQFDGCDILINCAGRAYPRYFEDI
                     SIKQFEETMKINMFGVWNTCWFLVPHMKANGGYIVNTSSVAGLVGVFGYTDYSASKFA
                     IIGFSEALKSELKRYNITVSVLCPPDTDTPGFAVENKTKPDETKEISKSAKLLTPDEV
                     AAALVQGMKKKKFIILANFDSELTWFVKRYAPWLVELIMNSKITGIQKKKKKTKK"
     gene            685..1086
                     /locus_tag="PLJ75_13075"
     CDS             685..1086
                     /locus_tag="PLJ75_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06189.1"
                     /translation="MSLKTAGVLSIVTGVGILLFWIAFFTVGLAPDIPPACYFSYEYA
                     FPLPDTILSLALIIGGIYALQRHAMKIILLPACGGLIFLGLLDMAFNYQNGMYTITVM
                     DGVLNAFINIWCVVFGLCAILTLQREYVKKS"
     gene            1146..1220
                     /locus_tag="PLJ75_13080"
     tRNA            1146..1220
                     /locus_tag="PLJ75_13080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1181..1183,aa:Val,seq:gac)
     gene            1246..>2354
                     /locus_tag="PLJ75_13085"
     CDS             1246..>2354
                     /locus_tag="PLJ75_13085"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPD06190.1"
                     /translation="MATLQLDNGKTVEVPDGKTLLDVLPQLNSKAKPVAARINGLLKD
                     LRSPVTNDATVELISFDSPEGRKVFWHSASHLMAQAVKRLYPQVKVSIGPAIDNGFYY
                     DFDKPGGFSHEDLEKIETMMQSIVNENIPIIRKELSRQEAIELFKASGELYKVELLQE
                     LSDNIVSLYEQGDFVDLCRGPHVPGTGYIKAFKLLSIAGAYWRGDEKNTMLQRIYGIA
                     FPDDRMLKEHLDFLEEVKKRDHRNLGRDLDLFSIHEDAGGGLIYWHPNGGRLRATLEQ
                     WWRDEHFKHGYEILYTPHIGRATLWETSGHLDFYKENMYSPMDIDGNDYYIKPMNCPF
                     HIKIYETSLHSYRDLPLRWAELGTVYRYEKSGVLHG"
BASE COUNT          690 a          424 c          561 g          679 t
ORIGIN      
        1 catggtacca gtgtgatgtt tcagattata cgcaggttaa aaagactttt acccaggctt
       61 tgcgtcagtt tgatgggtgt gatatcctta tcaactgtgc aggcagggca tatccacgat
      121 attttgagga tatctccatc aagcaatttg aagagaccat gaagattaat atgtttggtg
      181 tgtggaatac ctgctggttt ttggttccac acatgaaggc aaatggaggg tacattgtta
      241 acacatcatc agtggcaggg cttgtaggag tttttggata taccgattac agcgcctcaa
      301 aatttgccat cataggattt tcagaggctc tgaaaagcga gcttaaacgg tataacatca
      361 cggtatcggt gctatgcccc cctgatacgg atacccctgg ttttgctgtt gaaaacaaaa
      421 caaaaccgga tgaaacaaaa gagatatcta aatcagctaa attgttaaca ccggatgagg
      481 ttgcggctgc actggtgcag ggaatgaaga aaaaaaagtt tattatcctg gcaaattttg
      541 acagcgagtt gacatggttt gtaaaacgat atgcgccctg gcttgttgag ctgattatga
      601 acagcaagat aacgggcatt caaaagaaaa aaaagaagac aaaaaaatag tacaataata
      661 aaccataata aaaggaggaa cgatatgtca ttgaaaactg ctggtgtttt atctattgta
      721 acaggtgttg gtatcctgtt attctggatt gcttttttta ctgttgggct ggcaccggat
      781 atcccgcctg catgttattt ttcatatgaa tatgcattcc cattaccgga taccatattg
      841 tcgctggcat tgataattgg cggcatatat gccttacaac gacatgcaat gaagattata
      901 ctgttgccgg cctgtggcgg gcttatcttt ttggggttac ttgacatggc tttcaactat
      961 caaaatggca tgtacaccat cacggttatg gatggcgtgt taaacgcttt tatcaatatt
     1021 tggtgtgttg tttttggtct atgtgctata ttgactttac agcgggaata tgtgaaaaag
     1081 tcttgacagc ttattagcac tttcttaact aaagatttat ttatattttt tttggcgata
     1141 gttatgggcg attagctcag cctggttaga gtgcctgctc gacatgcagg aggtcactgg
     1201 ttcaactcca gtatcgccca atttatttta ggattggcaa aaataatggc aactttgcaa
     1261 ttagacaacg ggaagacagt tgaggtgccg gatgggaaga cactgcttga tgtgcttcca
     1321 caattaaaca gcaaagcaaa accggttgcg gcacgtatca atgggctttt aaaagatttg
     1381 cgctcaccag ttaccaatga tgcaaccgta gagcttatat catttgatag tcctgaaggg
     1441 cgtaaagttt tctggcattc tgcatcgcac cttatggcac aggcggtaaa gaggctatat
     1501 ccccaggtga aggtatcgat tggtccggct attgacaatg gtttctatta cgattttgat
     1561 aaacccggtg gtttttctca tgaggattta gagaagattg aaacaatgat gcaatccata
     1621 gtcaatgaaa atatcccgat tatacgaaag gaactatcgc ggcaggaggc aatagaactg
     1681 tttaaggcgt caggtgaact atataaagtt gagctgctac aggaactatc ggacaacatt
     1741 gtgtcgttgt atgaacaggg ggattttgtt gatttatgcc gtggaccaca tgtacccggt
     1801 accggatata tcaaagcatt taagcttttg agtattgccg gcgcgtattg gcgcggtgac
     1861 gaaaaaaata caatgcttca gcgtatttat ggcattgcgt tcccggatga tagaatgctg
     1921 aaagaacatc ttgatttttt agaagaggta aaaaaacggg atcaccgtaa tttaggcagg
     1981 gatttagacc tgttcagcat tcacgaagat gctggcggcg gtttgatata ctggcatccc
     2041 aatggcggaa ggctgagagc aactctggag caatggtggc gtgatgagca ctttaaacat
     2101 ggatacgaaa ttttgtatac tccgcatata gggcgcgcca cactatggga aacatcaggg
     2161 catctggact tttataaaga aaatatgtat tcgcccatgg atattgatgg caatgattac
     2221 tatataaaac caatgaattg cccattccat ataaagatat acgaaacaag cctgcattcg
     2281 taccgtgatc tgccgctacg atgggctgag cttggaaccg tataccgcta tgaaaaatcc
     2341 ggtgtattgc atgg
//
LOCUS       DAOOJH010000206         2326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_36178_length_2326_cov_11.680757, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000206 DAOOJH010000000
VERSION     DAOOJH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36178_length_2326_cov_11.680757"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLJ75_13090"
     CDS             complement(<1..905)
                     /locus_tag="PLJ75_13090"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPD06191.1"
                     /translation="MNFEEYFDNDTIQIENTIMASDLSKIDTAMPSTIHIIPVRYKPI
                     FPGIITPLIIAKPRFMHSIDEALNISRSIGIVVLKDDDVEDVALDDIEMFGTAAKIIK
                     KINLPDESINVLINSVCRFKINKITEEKSNILAKVDYIYDKANPKNVEIKALTRAILS
                     QLKILSESNPLFTEEMKLTMLNVDEPGRIADFVTSILNLEKHEYQNILETINVKTRLE
                     KVLVLLQKELEVLSVQRKIQNRINEKIYKQQKDYFLREQLKIIKQELGVEDDRHREIE
                     QMRSSAEALHLNHESREKIFEEINRL"
     gene            complement(962..1594)
                     /locus_tag="PLJ75_13095"
     CDS             complement(962..1594)
                     /locus_tag="PLJ75_13095"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPD06192.1"
                     /translation="MFSEIIKQLPSYFESQNNPVIYLLLFFSAILENLIPPIPGDVVT
                     AFGAFMVGTGKLHFIGVYLSTTLGATTGFTLLFLIGKFLGKEFMLNSKFNFFKYDNIL
                     KAELWVQKYGNWLVLSNRFLPGIRSVISLTAGFSNLPTHTVLLFSLLSASAWNLIIIY
                     LGYSLGNNWFIVQNKISYILSQYSKVLLFAGCIVLLVYIAIKLKKYVAKN"
     gene            1772..2305
                     /locus_tag="PLJ75_13100"
     CDS             1772..2305
                     /locus_tag="PLJ75_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06193.1"
                     /translation="MKKLLCSIFIVMMIAGCTTTIKHKQDEDPFALENQKFLEQTTGQ
                     EAFRVLITPESYKVVQVNFKDKIARIADESGDKYILEEIAQYNQIDEAREGIATVWLF
                     PDSGGIMKIRPTQLTFLVELDNLMIEDLQRWTFSFPGKSVEPTKFDVKYRIVLRKKLS
                     DEEIILQIREKIKDKTQ"
BASE COUNT          772 a          390 c          393 g          771 t
ORIGIN      
        1 agcctgttta tttcctcaaa aatcttttct ctggattcat ggttaagatg caatgcttcc
       61 gctgaagatc tcatctgttc aatttcacga tgcctgtcat cctctactcc caattcctgt
      121 tttatgattt ttaattgttc acgtaaaaaa taatctttct gctgtttgta tatcttctca
      181 ttgatacgat tttgtatttt tcgctgaacg gataaaacct ccagttcttt ttgtaaaaga
      241 accagcactt tttccagacg tgtttttaca ttgattgttt ccagtatatt ttgatattcg
      301 tgtttttcca gatttaagat agatgtcaca aaatctgcaa ttcgtcctgg ttcatcaaca
      361 ttgagcattg tcagcttcat ctcttcagta aacagaggat tgctttcaga taatatttta
      421 agctgactta atattgcgcg tgttaaagct ttgatctcta cattttttgg atttgcctta
      481 tcgtagatat agtcaacttt tgccaggatg ttacttttct cttcagttat tttgttgatt
      541 ttaaacctgc aaacactatt gatgagcaca ttgatgcttt catcaggaag attaattttt
      601 ttaattatct ttgcagctgt gccaaacatc tctatatcat ctaatgcaac atcctcaacg
      661 tcatcatctt ttagtacaac gataccaata gaccttgaga tgtttaatgc ctcatcaatt
      721 gagtgcataa atcttggttt tgctataata agcggggtaa taataccagg aaatataggt
      781 ttatagcgaa ccggtataat gtgaattgtt gatggcatag cggtatctat tttggaaaga
      841 tcgcttgcca ttatggtatt ttctatctgt attgtgtcgt tgtcaaaata ttcttcaaaa
      901 ttcattcaat gtctccataa aatcataaac ataacacagg aaatataata tatatgcata
      961 actaattttt tgctacatat ttttttaatt taattgcgat atataccaat aaaactatac
     1021 agccggcaaa taataatact ttgctatatt gtgacaaaat gtaacttatt ttgttttgca
     1081 caataaacca gttattacct aacgaatagc caagatatat aataattaaa ttccatgccg
     1141 aagcgcttaa aagagaaaac agcaaaactg tatgtgtagg taaattagaa aaaccagcag
     1201 ttaaagaaat gacactgcgg atacctggca aaaagcggtt tgaaagcacc agccaatttc
     1261 catatttttg tacccatagt tcagccttaa gtatattgtc atatttaaaa aaattgaatt
     1321 tactgttcag cataaactct ttccctaaaa attttccaat taaaaatagt agcgtaaaac
     1381 ccgtagtagc accaagggtt gttgatagat aaacgcctat aaaatgtaat ttccctgttc
     1441 caaccataaa tgctccaaat gcagtaacaa cgtcacccgg aataggcggt atgaggtttt
     1501 ccagaattgc actgaaaaaa agtaacaaat aaataacagg attattctgc gactcaaagt
     1561 atgaagggag ctgtttaatt atctcactga acatataatt ttacttgaaa ttattgtata
     1621 atacaatata gtttggtgta ttactaaaaa atatttttca tttgtaatga gcgtacacaa
     1681 taggtgatat aataattttt tatacagtga tgtcaagata gtatagttgc tttattttgt
     1741 caagtaagct ggaaagattt atggagatat tatgaaaaag ttactatgtt ccattttcat
     1801 tgtaatgatg atagcaggat gcacaacaac tatcaaacac aaacaagatg aagacccgtt
     1861 tgctcttgaa aatcaaaaat tccttgagca aacaaccggg caggaagcct tcagggtcct
     1921 tataacccct gaaagctaca aggttgttca ggttaacttt aaagataaga ttgcgcgtat
     1981 tgctgatgaa agtggtgata aatatatcct tgaagagata gcacaatata atcaaataga
     2041 tgaagcaagg gagggcattg caacggtttg gcttttcccc gattcaggcg gtatcatgaa
     2101 gatacggcca acgcagttaa cttttctggt ggagcttgac aatttaatga tagaggacct
     2161 ccagcgctgg acattttcat ttccgggcaa aagcgttgaa cctactaaat ttgatgttaa
     2221 atacaggata gttttacgga aaaaactttc cgatgaagag ataatactgc agatacgaga
     2281 aaagataaaa gacaaaacgc agtaacaaca gatagtggaa ggttat
//
LOCUS       DAOOJH010000207         2324 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_36218_length_2324_cov_7.628911, whole genome shotgun sequence.
ACCESSION   DAOOJH010000207 DAOOJH010000000
VERSION     DAOOJH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36218_length_2324_cov_7.628911"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLJ75_13105"
     CDS             complement(<1..340)
                     /locus_tag="PLJ75_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06194.1"
                     /translation="MESCGINIGSTSVKVVLCNNNEIQKFCVLPHEGDVPGTLGKIVA
                     GIRIPDNAKAIVTGNEGRFMINLPRVIESVCIEEVLRHLVVDPVAVVSMGGEDLVVYT
                     IGEDKKIKTSF"
     gene            complement(416..1492)
                     /locus_tag="PLJ75_13110"
     CDS             complement(416..1492)
                     /locus_tag="PLJ75_13110"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HPD06195.1"
                     /translation="MKYVGVDLHKNQFTAYDSSTGRYRNYGSNAIGIMSFIRDCKDTT
                     TGEQIVVGVESTGNTRYFKRLLEAERIQVKVINTMKFKVITESVKKTDKHDASTIAEF
                     LGKDMLPEAWLCSEYSERLRRLLHVRKTLVKAIVTTKNQIHGMVTAYGYEDKKSSLQS
                     KRGRHMIMNTLASTEIGLVVQLLMDTIESLSENVKNIEKKIEELVEGDTRIELLMSIP
                     GCGRITAWTIRAYTDDMNRFKSAQQYAAYVGVVPYVHNSNTLIRHGRITKHGPEPLRT
                     AFVQLVMGINRMRALHNYRIMKRYRFIKENKGSGKAIIATARKLTHIVWHILTNNEPF
                     NPDLQLRSSWESNRHVQFEEKRFA"
     gene            complement(2002..>2324)
                     /locus_tag="PLJ75_13115"
     CDS             complement(2002..>2324)
                     /locus_tag="PLJ75_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPD06196.1"
                     /translation="TDVVFAVDSIPAVLAISQDPFIVYTSNVMAILGLRALYFALAAV
                     MRLFRFLHYGLSVILIYVGCKMIAAQLGYHIPTEISLGVIALVLTLSIVLSIYIPEKK
                     NNAN"
BASE COUNT          612 a          547 c          381 g          784 t
ORIGIN      
        1 agaagctggt cttgattttt ttatcttcac cgatggtgta aacaacaaga tcttcgccgc
       61 ccattgaaac aactgcaaca gggtctacta ctaaatggcg caatacctct tcaatgcata
      121 ccgattcaat aacacggggc agatttatca taaacctccc ttcattaccg gtaactatcg
      181 ctttagcatt atcaggaatt cttattccgg ctacaatttt gccaagggta cctggcacat
      241 ctccctcatg aggaaggaca caaaactttt gtatttcatt attattacat aaaacaacct
      301 tgacgcttgt tgaaccaata tttattccgc aactttccat attgtcttct cctataaaaa
      361 gtcaaaaatt tttaagaccc tgttcaaaat tataaaaaat tatatagtaa tcaggctatg
      421 caaatcgctt ttcttcaaac tgcacatgcc tgttactttc ccatgacgac ctcaattgca
      481 aatcaggatt aaatggttca ttgttcgtta atatatgcca tacgatatgc gttagctttc
      541 tggcagttgc tattattgct ttccctgatc ccttgttttc ttttatgaat ctataccgtt
      601 tcataattcg ataattgtgc aatgcccgca tacggtttat tcccatcact aattgcacaa
      661 atgctgtccg taacggttct ggcccatgtt tggttatcct cccatgccgt atgagtgtat
      721 ttgagttgtg cacatacggc actaccccca catatgctgc atactgctgg gcacttttaa
      781 atcgattcat atcatccgta taggctcgta ttgtccacgc tgttatacgc ccacagcctg
      841 gtatactcat taaaagctct atgcgcgtat ccccctctac cagttcctca atctttttct
      901 caatgttctt gacattctcg cttaatgatt ctatcgtatc catcaacagc tggactacga
      961 gtcctatttc tgttgatgcg agggtgttca taatcatgtg acgccctcgt ttgctttgta
     1021 acgaggactt cttatcttca tatccatatg cggttaccat cccatgtatc tggttctttg
     1081 ttgttactat cgcttttact aatgtcttgc gaacatgtaa taaccgccgt agccgttcac
     1141 tgtattctga acatagccat gcctcaggaa gcatgtcctt tcctaaaaat tcagctattg
     1201 tcgatgcatc atgtttgtcc gtcttcttca ctgattcagt tattacttta aatttcatcg
     1261 tattaataac tttgacctgt atcctttctg cttccagtaa acgtttaaaa tagcgtgtgt
     1321 tccctgttga ttcaacacct accactatct gttcacccgt agtagtatcc ttacaatcac
     1381 ggatgaaact cattatccca attgcgtttg agccatagtt gcggtagcgc cctgtactgc
     1441 tatcatatgc cgtaaactga ttcttgtgta aatcgactcc tacatatttc atatagtacc
     1501 ccctgttaaa agtattgtga acagggcctc ttcggtgtcg ctccattctc ctatccgacc
     1561 tcgcaggtca ttgaatggta cggttacagt ccctcattct ccttcccggc ctttttgacc
     1621 acatactatg acgaacggta acctgcctgt tcatagtgac attatgaaca gattttgctt
     1681 ctcaccgcaa ctattttgac ttttttatca ttcctccttt gttctacggt caatgtttct
     1741 atttttgtgt tttgtcaatt tatttttata ggcaactatg aaaatagatt tatttacctc
     1801 gcccccggag cctctccttc tcaaggcgag gagagtattg tttccggttt atacaatttt
     1861 cgtttcccat gggtcgccac taatttgcca ttgttaactc ccccgaaact atctttctca
     1921 tatgattatc ctaccaattg tactttgaaa aaaaatggct ctgctgcaat gtaattgctt
     1981 aatgggttta tatgaaaatt tttagtttgc attattcttt ttttccggaa tatatataga
     2041 taatacaatt gaaagtgtca gaaccaatgc aataacccca agagagatct ccgttgggat
     2101 atggtatcca agctgggctg caatcatctt gcaaccaaca tagatcagta taacagaaag
     2161 accataatgc aaaaaacgga acagccgcat aactgctgct aaagcaaaat agagtgctcg
     2221 caatccaagt attgccatga catttgatgt atacacaata aatggatcct ggcttatagc
     2281 cagcactgct ggtattgaat ccactgcaaa cacaacatcg gttg
//
LOCUS       DAOOJH010000208         2319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_36331_length_2319_cov_9.793286, whole genome shotgun sequence.
ACCESSION   DAOOJH010000208 DAOOJH010000000
VERSION     DAOOJH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36331_length_2319_cov_9.793286"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..488
                     /locus_tag="PLJ75_13120"
     CDS             174..488
                     /locus_tag="PLJ75_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06197.1"
                     /translation="MNGDVLIQNIINLFVMAIILEAAVMAIFSMSTFRDMKMTRAIEA
                     SRDVIILLSAFFLCYKVDVLHVFRKTGLNIPVLFDIIISTLVLTRMTNFVRQVFNRLR
                     QD"
     gene            complement(684..980)
                     /locus_tag="PLJ75_13125"
     CDS             complement(684..980)
                     /locus_tag="PLJ75_13125"
                     /inference="COORDINATES: protein motif:HMM:NF015959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HPD06198.1"
                     /translation="MNDIITEVRVFPKDNLGKTLGFANITLLDKFVVKNLRIVKGDKG
                     IFIGMPSNKRKNGEYIDLFFPITQEARDLITSAIIDKYNELMNTSYPVENKVKQ"
     gene            complement(1565..1909)
                     /locus_tag="PLJ75_13130"
     CDS             complement(1565..1909)
                     /locus_tag="PLJ75_13130"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD06199.1"
                     /translation="MRQIVSSGSVKACWVNNEDVINTLKIVAVQAKQKFPEIIEIWLF
                     GSFAKGEATGISDIDIFIVAQTKIDNPLERIKPYYNYFSDCLKIAVDIIVAKPDEKKY
                     HEEMLNDAILLA"
     gene            complement(1972..2151)
                     /locus_tag="PLJ75_13135"
     CDS             complement(1972..2151)
                     /locus_tag="PLJ75_13135"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HPD06200.1"
                     /translation="MQQASEKVLKALSMKSGLTIWGHSLTEILNILAGRINMPDDITS
                     SAKLLDIYYIPTRLS"
BASE COUNT          770 a          373 c          377 g          799 t
ORIGIN      
        1 tacaacacat aaaatcaaag ttgatagaat ttttataaca aaggagatca tacatagtga
       61 tgtgaggata gtaagtaaaa aaaatattat tgacattaat aattttatat tatacaaaac
      121 aaagtaatta tcatggatgg agtacaaaaa taattaacag gaggttttga tacatgaatg
      181 gcgatgtgct aattcaaaat attatcaatc tttttgtaat ggcaataata ctggaagctg
      241 ccgttatggc aatattttcg atgtcaacat ttcgtgatat gaaaatgaca cgcgctattg
      301 aagcaagcag ggatgtaata attttattat ctgctttttt cctatgttat aaagttgatg
      361 tattacatgt tttcaggaaa acagggttga acatacccgt gctgtttgat ataattatca
      421 gtacattagt gttaacacgt atgaccaatt ttgttcgaca ggtattcaat cggttgcggc
      481 aggattaaat gaagagtgta agccgggtca cggtaacgtt atcatctctt accataccaa
      541 aacctgtcgc ttcaaatttt taaatggata aaactactgg tttaaaaaga taaaacgcaa
      601 gtttattaat taaaaaccat cacatcatct ataaagagat gtaaagactt tttgcaatga
      661 ttccgaagcg ggctttatag caattactgt ttaactttgt tctctacagg atagctggta
      721 ttcatcaact cattgtactt gtcaattatt gcagatgtga tgaggtccct tgcttcctga
      781 gtaatgggaa agaacaggtc aatatactca ccattttttc gcttgttaga cggcatccct
      841 ataaaaattc ccttgtcacc tttaactata cgcagatttt ttactacaaa tttgtctaac
      901 aaagtgatat tggcaaaccc cagagttttc cctaaattat ccttgggaaa taccctaact
      961 tcggtaataa tgtcattcat gtgatcacca ccataccgta tgttttgttg atagagtata
     1021 cacgtaaggc ttataataag tcaagcaaaa taatttttta aaaaacaatt gggcgatagt
     1081 tactgttaat tgatatcata gtttaaaaga aacatttatg catttcgtaa aaagcaagta
     1141 ccatgtaaaa tagcacaaga atgtgtcact tatgggtagt tggtactttt gattagtatt
     1201 gtatctgtaa aacaaccact ttttatctga aaaataattg caattgttaa tttaatcaaa
     1261 tttaaatagt atgtaaaaaa cgattcacat atttctaaat aattgctttt taattgatct
     1321 aaataaccat gatctttctg gcaaattgaa gataataaaa aatgacagat taattgtatc
     1381 aatcatggag ggagtaattt tgctgttgtt aaatgctcac aagtgttcaa tattcagctt
     1441 ggtgatgtca tggtatttta tgaaaaccta atcaatcatg gttaccttta ttgaaaaaac
     1501 attgtagcaa tattgattta gtgataacag gtgaacatcc aaaatatagt tatgaaataa
     1561 tcatttatgc aagaaggatg gcatcgttaa gcatttcttc atgatacttc ttctcgtcgg
     1621 gttttgctac tataatatca acagcaatct tcaagcaatc tgaaaagtag ttatagtatg
     1681 gcttaatcct ttccaatgga ttatctattt tggtttgcgc tactatgaat atatcaatat
     1741 cacttatacc tgtggcttcg cccttagcaa atgaaccaaa tagccagatt tcaataattt
     1801 cggggaactt ttgttttgcc tgtacagcaa caatttttaa tgtattaata acgtcttcat
     1861 tgtttaccca gcatgctttc acagaacctg atgatactat ctgccgcatc atgtgcctcc
     1921 atagcctgtt tttttgtaaa ataatcagcc ggtttgccgg atgcaaatcc attaggataa
     1981 tcgtgttgga atataataaa tatccagaag ttttgcgctg gaggtaatat cgtcaggcat
     2041 gttaattctg ccagctaaaa tattcagaat ctctgttaat gaatgtcccc atatcgttaa
     2101 accggacttc atggataatg cttttaaaac tttttcagat gcctgttgca aactaaagca
     2161 agcccattca tagtattcgt attctatatc tatgttggct ctttctcaat ctcttttccc
     2221 ctgttcaaac cagtcgttcc atctattcat ttaaagattc cttttattga tagttgctat
     2281 cgcttaagta aattatagag taatatgtat acatataca
//
LOCUS       DAOOJH010000209         2314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_36444_length_2314_cov_7.239044, whole genome shotgun sequence.
ACCESSION   DAOOJH010000209 DAOOJH010000000
VERSION     DAOOJH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36444_length_2314_cov_7.239044"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PLJ75_13140"
     CDS             complement(<1..356)
                     /locus_tag="PLJ75_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06201.1"
                     /translation="MNIVFYTSGISGTGRVVTGISIYNALKRKQIQCNYTMVTHSPAG
                     FLAQDSGIEHVQLPLEDEHQLSRKHFHSSVLYHTLTVLKPDILIVYLQWFTILTFLDD
                     LKCIKLFIAHQVNKNF"
     gene            complement(360..1607)
                     /locus_tag="PLJ75_13145"
     CDS             complement(360..1607)
                     /locus_tag="PLJ75_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06202.1"
                     /translation="MKSNKVFLSLLLIFSIITFFNCSGDNGTSIIIINVGLQNQQTHN
                     SFFYKLINFIQQPVYADPPSHIQSITINITAADFSPITRTYTPPYGIFRIEVPSGIQR
                     AIEVLASTPSATIRGFVIVDLKPGEQTVDIAMHLYSTKILIPDYNSHRLVQIDDMTGS
                     GFRDINGGTISFPGGTIYPYDVDFDDIGRIYIANYGFSTEQNVVIRLDDIEDTTYTSL
                     GTGGLNGIRSVAVDRENKLVYYATSSQLYRCDLNGSNLKSDYTMTDISFIKSIAYHNS
                     FLYIACTYDSNYRIVKYDTTANGQIAGYSLASLHSAWDVIIKNDLCIVADDDESTSND
                     SIAILDPDDLSLFNKYGTGTSVPSPLPGQFWGPRRFVAILNDRFYLIDERDGALDNLV
                     SFSDLNFSNWERYNNSFMFFSFC"
     gene            complement(1777..>2314)
                     /locus_tag="PLJ75_13150"
     CDS             complement(1777..>2314)
                     /locus_tag="PLJ75_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06203.1"
                     /translation="PMFRYNTYFGINTVHFFDLMQANEKERLPLGAIARATIATLISK
                     WAAKQKKLEKPLGEYGLQLFNRLDALKFAAWIDNDGFPTIVPVIQAKASDPGTIVFSP
                     KAYCQELSNLQQGTPIALYALTLKMENILTRGIFKGYKRYAGIQLGACDINYVYNSMP
                     PVHGQIYPKIPLAPVKEF"
BASE COUNT          749 a          430 c          398 g          737 t
ORIGIN      
        1 aaaaagtttt tatttacctg atgcgcgata aaaagtttga tacatttcaa atcatctaaa
       61 aaagtaagta tggtaaacca ttgcaggtat acaatcaaaa tatcaggctt tagtactgtt
      121 aatgtatgat ataaaacaga tgaatgaaaa tgttttcgtg ataactggtg ctcatcctca
      181 agaggtagtt gtacatgttc aatgcctgaa tcctgcgcaa gaaagcctgc agggctatga
      241 gtaaccattg tataattaca ctggatttgc ttacgtttaa gagcattgta aattgaaata
      301 cctgttacta ccctgccggt tcctgaaata ccggatgtat aaaaaacaat attcactttt
      361 taacagaatg aaaaaaacat aaagctgtta ttataccgtt cccagttgct gaaatttaaa
      421 tcagaaaacg agacaagatt atccaacgcc ccatcccttt catcaatcaa atagaatcta
      481 tcatttaata ttgcaacaaa ccggcgtggg ccccagaatt gtccaggcaa aggtgatggg
      541 acgcttgtac ctgttccata tttattgaat aatgataaat catctggatc caaaatagct
      601 atcgaatcat tggaagtact ttcatcatca tctgctacaa tacataggtc atttttaatg
      661 atgacatccc atgctgaatg caaagatgcc aaagaataac ccgcaatttg tccatttgct
      721 gttgtatcat atttaacaat tcgataattt gaatcatagg tacatgctat atatagaaag
      781 ctattatgat acgcaatgct ctttataaat gatatatctg tcatggtata atcacttttt
      841 aaattgctgc cattaagatc gcagcgataa agctgactgc ttgttgcata atacaccagt
      901 ttattttccc tatcaactgc tactgatcta ataccgttca aaccaccagt tcccaatgaa
      961 gtataagtag tatcttctat gtcatcaaga cgtatcacaa cattctgctc tgtagaaaaa
     1021 ccataatttg cgatatatat cctgccaata tcatcgaaat ccacatcata gggatatata
     1081 gtacctcctg gaaaactaat agtacctcca tttatatcac gaaatccgct ccccgtcata
     1141 tcatcaattt gcacaagcct atgactatta taatcaggta tcagtatctt tgtgctgtat
     1201 aaatgcatgg caatgtctac tgtttgttcg cccggtttta aatcaacgat tacaaatccg
     1261 cgaatagtag cggaaggagt gctggctaaa acttcaatag ctcgctgtat tcctgaaggg
     1321 acctcaattc taaagattcc atacggtggt gtatatgtcc ttgtgatagg agaaaagtcg
     1381 gcagcagtaa tattaattgt tatactttgt atatgagatg gtggatctgc atatacgggt
     1441 tgctgaataa agttgattag cttataaaaa aatgagttgt gtgtctgttg gttctgtaag
     1501 cctacattaa ttattatgat tgaagtaccg ttgtctccgc tgcagttaaa aaaagttatt
     1561 attgagaata ttaacagtaa tgacaaaaat actttgtttg atttcattgc aaacctcgta
     1621 atctcaaata ttaatattta tgagccagga agagattagt aattatatac aattccaaat
     1681 aaaatatata tagtacaatg tcaactatta ttaataataa cgattaataa tattggcagc
     1741 tttcaatgct acagccattt atttttttta aaagcatcaa aattccttta ccggtgcaag
     1801 cggtatttta gggtaaatct gcccatgtac cggcggcatg gagttgtaca catagttgat
     1861 atcgcatgct cccaattgta tgccagcata tcgtttatac cctttaaaaa ttcctctggt
     1921 taaaatattt tccatcttca gcgtgagtgc atacaatgca attggtgtgc cttgctgtaa
     1981 atttgatagt tcctgacaat acgcctttgg cgagaaaact attgtacccg gatccgaagc
     2041 ctttgcctga attaccggca caattgttgg aaaaccatca ttgtcaatcc aagcggcaaa
     2101 tttcaaagca tctaaccggt taaaaagttg taaaccatac tcgcctaacg gcttttctaa
     2161 ttttttttgt tttgcagccc attttgaaat tagtgttgct atggttgctc tggcgatagc
     2221 tcctaaaggc agtcgctcct tttcattagc ctgcatcaga tcaaaaaagt gaaccgtgtt
     2281 aatgccaaag taggtattat agcgaaacat tgga
//
LOCUS       DAOOJH010000210         2266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_37515_length_2266_cov_8.790592, whole genome shotgun sequence.
ACCESSION   DAOOJH010000210 DAOOJH010000000
VERSION     DAOOJH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37515_length_2266_cov_8.790592"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..364
                     /locus_tag="PLJ75_13155"
     CDS             125..364
                     /locus_tag="PLJ75_13155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06204.1"
                     /translation="MISQYRGSLSVVGLQLPERNVIGINAIILLFRGKANSHCETIVY
                     KMSLVDAKNILYVLYERSDSLGLPYLNMTLWEMYC"
     gene            550..894
                     /locus_tag="PLJ75_13160"
     CDS             550..894
                     /locus_tag="PLJ75_13160"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPD06205.1"
                     /translation="MKLIVKKWFILSIAIYLASVLLNGFSAETATAIIIASALLGIFN
                     VFIKPVLIFITLPINLLTLGLFTFVINALLLYMVSSIVKGIYLDSFWSALLASIIISI
                     LSMIINWIFADR"
     gene            927..2093
                     /locus_tag="PLJ75_13165"
     CDS             927..2093
                     /locus_tag="PLJ75_13165"
                     /inference="COORDINATES: protein motif:HMM:NF024925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD06206.1"
                     /translation="MFIVSIGAGKNQLPLIRACKDLQCKIVGVDINPHAEGFPLCDFK
                     IIESIYNYHEIYQSLKELLVFDDIAAIVTRSYGEALRTVSYLCQQFKIPHINYAMIDY
                     CIDKSKFRTIANKTITMPHCFTMGKNDKGNILTLSDITFPCIIKPSQGHAKHNVQLID
                     SVQHLKQSIASMKHSTIMVEEYIEGDEIIVIGFVINKKFYICDISDKVLNAKPYFVDR
                     MHLLPSTYYHYHNQLTSIGQQIADLFLLESTPLLMECVISNNTIYVIEAACEFGGEYL
                     ADYAIPARLHYDIFKAFITGITTGKVELPKKSTTASVVKYIMGRQGRLTHYKQVENKK
                     NCIHNEIFATIGDAIQFPENNHNRLGVIVTKGKTVEKAIKTANTVLEQMEIIIE"
     gene            2095..>2266
                     /locus_tag="PLJ75_13170"
     CDS             2095..>2266
                     /locus_tag="PLJ75_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD06207.1"
                     /translation="MNNKTSQSWNNHYTRDSSMLAYPDEQLVRMIKKNTINKQRALDA
                     GCGTGRHVKLLNE"
BASE COUNT          787 a          367 c          396 g          716 t
ORIGIN      
        1 aaacagccat gcaattttac aaaaaatata aagatgaatt ttcactttta tggaaagaaa
       61 cagaattgga acaacaatac caggttattt cggttgattt atatccacaa tccatatggc
      121 atttttgatt tcacagtata gaggaagttt gtctgttgtt gggctgcaat taccggaaag
      181 aaatgttatt ggaataaatg ccattatact attattcaga ggcaaagcca atagtcattg
      241 tgaaacgata gtgtataaga tgagtttagt cgatgcaaaa aatatccttt atgtccttta
      301 tgaaagaagc gattctttgg gactacccta tctcaacatg acactatggg agatgtattg
      361 ttaagcacgt tgcttcctta tcatgatgac tcttacgagg ttattcttaa catttatgca
      421 gtctaaattt ggaatacaat gccataataa gtttgggatg acttttttat tgaatatgtg
      481 ttctggatgt aatcgtacaa caaaacgtac atagtaaact aataaattta attttgagga
      541 aatgtaaaaa tgaagttaat tgtaaagaaa tggtttattc tctcaattgc tatttattta
      601 gcttcagtgc tgctaaatgg attttcagca gaaacagcta cagcaataat tattgcatca
      661 gcattgctgg ggattttcaa tgtttttatt aaacctgtat tgatctttat aacattaccc
      721 atcaatcttt taacacttgg cttatttact tttgtgatca atgcattact tttgtatatg
      781 gtttcatcga ttgtaaaggg aatttatctt gattcattct ggtcggcttt actggcatcg
      841 ataatcataa gtatactatc gatgataata aactggatat ttgccgatag ataaagagaa
      901 acaattactg tatagttatt aaatctatgt ttatagtatc aattggtgca gggaaaaatc
      961 aattaccgtt aattcgggca tgcaaggatt tgcaatgtaa gattgttggc gtggatataa
     1021 atccccatgc tgagggtttt ccattatgtg attttaaaat tattgaatct atttataact
     1081 atcacgaaat ttaccaatcc ttgaaagaat tgcttgtttt tgatgacatt gctgccatcg
     1141 ttacccgatc gtatggcgaa gcattacgaa cagtatcgta tctatgtcag cagtttaaaa
     1201 taccacatat taattacgct atgattgatt attgcattga taaatctaaa tttagaacta
     1261 tcgccaataa aaccattacg atgcctcatt gttttactat gggtaaaaat gataaaggga
     1321 acatactaac attaagcgac atcaccttcc cctgtattat aaaaccgtca caaggacatg
     1381 ccaaacacaa tgtgcaactt attgacagtg tccaacattt aaaacaaagt atagcttcaa
     1441 tgaagcacag tacaatcatg gttgaagaat atattgaagg tgatgaaatt attgtcatag
     1501 gatttgtcat caataaaaaa ttttatattt gcgacatcag tgataaggta ctgaatgcca
     1561 aaccatattt tgttgataga atgcatctgt tgccatcaac atattatcat tatcataatc
     1621 agctaaccag cattggacag cagatagctg atcttttttt actggaatca actccgttac
     1681 tgatggaatg tgttattagc aataacacca tctatgtgat tgaagcagcc tgtgagtttg
     1741 gtggtgagta tctggcagat tatgctatcc cagcccgttt acactatgat attttcaaag
     1801 cattcattac cggcattact actggcaaag tagaattacc caaaaaaagc actactgcat
     1861 cggttgttaa atacatcatg ggcaggcaag gacgtttaac tcactataaa caggttgaga
     1921 acaaaaaaaa ctgtatccat aatgagatat ttgcaaccat tggtgatgca atacaatttc
     1981 ctgaaaataa tcataaccgt ctgggagtaa tagtaacaaa aggaaagaca gttgaaaaag
     2041 ccattaaaac cgctaataca gtccttgaac aaatggaaat aataatagaa taacatgaac
     2101 aacaaaacat cgcaaagctg gaataaccat tatacccgtg acagttcaat gcttgcatat
     2161 cctgacgagc aacttgtgcg catgataaaa aaaaatacca taaataaaca aagagctctt
     2221 gatgctggat gcggaaccgg cagacatgta aagctgctca atgaaa
//
LOCUS       DAOOJH010000211         2251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_37862_length_2251_cov_8.193989, whole genome shotgun sequence.
ACCESSION   DAOOJH010000211 DAOOJH010000000
VERSION     DAOOJH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37862_length_2251_cov_8.193989"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..972
                     /locus_tag="PLJ75_13175"
     CDS             175..972
                     /locus_tag="PLJ75_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD06208.1"
                     /translation="MKLFECTSIVCSYTNIPVINNISLTINNGDFIGIIGPNGAGKST
                     LLKCLARILAPASGTIYYNGSDITAIPKKQYAQQVATVMEIEHLLPYTVESFVALGRF
                     PHSSFGRYDDEDISIIDNAIAYCSINTIKNKKLTQLSSGELQRVNIARALAQSTQCIL
                     LDEPISHLDIRHTIAIMDILKNLHRRGSTIITIIHDINIASEYCSRIIGLKQGTIIFD
                     DTPEKCITYHTIEKLFDCICLVYNNPLSNKPFVYPVPGHIQCNKHRQ"
     gene            complement(1020..>2251)
                     /locus_tag="PLJ75_13180"
     CDS             complement(1020..>2251)
                     /locus_tag="PLJ75_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD06209.1"
                     /translation="QGYFLFILLSLFQNDRKLTIATGAFAIIQYMLLVIIGIFVVHVP
                     VISGSAEWAHIVMDDEIGKIVLLIGFTAIAVAVLRNLQYFALAAISNEKNANEKAQRL
                     QQVIIEAQNTNKSLINVVKDQTDISNSLSLVAQDEAAMSEELSSIYEEETASIELINK
                     NAIKQTDETKIMESRINEFLDIQKMVIQTGEQMLDNIVRISDFSKKTETSLLEMSGIM
                     DIVTQSGKAMSSFIEVIHDITDQINLLSLNASIEAARAGEYGRGFAVVADEIGKLATA
                     TSDNAKEISSKLAMIIKDIQNSMSIVDATKIALNNVIDSISKSESNVNDVKGAIGKQQ
                     IAIQDIKEQSSLLNSLSHEILVAIAQQQTSMNESASSIQKLSEVAQVIAEKTSQMNGL
                     TGEIKNIAHRLSEVIQL"
BASE COUNT          676 a          482 c          368 g          725 t
ORIGIN      
        1 cgaacactct ttgctcattc ccctatcagc cattgcaggt ggcaccatgc ttgttgtagc
       61 cgacaccatt ggcaggacta tagtaatgcc ttatgaaata ccctgcggca ttattactgg
      121 tttttttggc ggcatatttt ttttgatcct gttactgaaa caaagggatg ctctatgaaa
      181 ctttttgaat gtaccagcat agtctgcagc tatacaaata tacctgttat aaacaacatc
      241 agtcttacta tcaataatgg tgattttata ggaattattg gtccaaacgg tgcaggtaaa
      301 tcaacattgc tcaaatgtct ggcacgtatc cttgctcctg cttccggtac catttactac
      361 aacggcagcg atatcacagc aatacctaaa aaacaatatg cgcaacaggt tgcaacggtg
      421 atggagattg aacacctgtt accctacact gttgaatcgt ttgttgcact ggggagattt
      481 ccccattcat catttgggag atatgatgat gaggatatat ctattattga taatgctatt
      541 gcttattgtt ccatcaatac cattaaaaac aaaaagttaa cacagctttc atcgggtgaa
      601 ctgcagcgtg ttaatattgc acgtgcatta gcacaaagca ctcaatgcat tcttcttgat
      661 gaaccaatat cacatcttga catccggcat acaatagcaa tcatggacat tttaaaaaat
      721 ttacacagga ggggtagcac cattattacg ataatacatg acatcaacat tgcatcggaa
      781 tactgtagca ggattattgg tttaaaacaa ggcaccataa tttttgatga cacacctgaa
      841 aagtgtatta cctatcatac catagaaaaa ttatttgatt gtatatgcct tgtctataac
      901 aatcctctat caaataaacc ctttgtgtac cccgtaccag gacatataca atgcaacaag
      961 catagacaat aaatgtataa ctacctgcta ttgccatata tactgtacac aaatattgtt
     1021 tacagctgaa ttacctctga caacctgtgt gcaatatttt ttatttcccc ggtaagtcca
     1081 ttcatctgtg aggtcttttc ggcaataacc tgagcaactt ctgaaagctt ctgtatagac
     1141 gaagcactct cattcatgga tgtttgttgc tgtgcaattg ccactaaaat ttcatgcgat
     1201 agtgaattga gcaacgatga ttgctctttt atatcctgta tggcaatctg ctgtttccca
     1261 attgcacctt ttacatcatt aacatttgat tcacttttac taatactatc aatcacattg
     1321 ttcaacgcta tctttgttgc atcaacaatt gacatgctat tctggatatc tttaataatc
     1381 attgctaatt tagatgatat ttcctttgca ttatcactgg ttgctgtggc taacttgcca
     1441 atttcatcag ccacaacagc aaaacctctt ccatattcac ccgcacgagc agcttcaatg
     1501 ctggcattca gcgaaagcaa gtttatctga tccgtaatgt catggataac ctcaataaaa
     1561 gagctcatgg ctttgccgct ttgtgtaacg atatccataa tacctgacat ctccaataaa
     1621 cttgtttcag tcttctttga aaaatcggaa atccttacaa tgttgtcaag catctgctcg
     1681 ccggtctgta taaccatctt ttgaatatca agaaattcat tgattcgtga ttccatgatt
     1741 ttcgtttcat cggtttgctt tatagcattt ttattgatca attctattga agccgtttct
     1801 tcttcataga tcgatgataa ctcttcgctc attgctgctt catcctgagc aaccagtgat
     1861 aagctgttgc tgatatcagt ttgatcctta accacattaa tgagtgattt attggtattc
     1921 tgcgcttcaa tgatgacctg ctgtaaccgc tgggcttttt cattagcatt tttttcatta
     1981 ctgatagctg ctaaagcaaa atattgcaaa tttcgcagta cagctaccgc aattgctgta
     2041 aaacctataa gcagaactat cttgcctatt tcatcatcca tgacaatatg agcccactct
     2101 gcactgccag aaataaccgg tacgtgaaca acaaatatgc caataataac caacagcata
     2161 tattgaatta ttgcaaatgc gcctgtagca atggtcaact tcctgtcatt ttgaaataac
     2221 gacagcagaa taaacaaaaa atacccctga a
//
LOCUS       DAOOJH010000212         2247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_37970_length_2247_cov_7.682026, whole genome shotgun sequence.
ACCESSION   DAOOJH010000212 DAOOJH010000000
VERSION     DAOOJH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37970_length_2247_cov_7.682026"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLJ75_13185"
     CDS             <1..329
                     /locus_tag="PLJ75_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06210.1"
                     /translation="NLTTLEPFTYTLAPIDELQTCRCLSVTKPLRTQDDRLSYSTIAD
                     IVDMELAGIAYASYKFGKQCYSIKYISDTGQHTTHETIMNNIINFSEKTVDTILEILF
                     SHALIK"
     gene            554..1369
                     /locus_tag="PLJ75_13190"
     CDS             554..1369
                     /locus_tag="PLJ75_13190"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD06211.1"
                     /translation="MQTKTKTTSQHNTFCSYCEYTGIVRNPNFEQIGEEPLVPCPRCV
                     LTKCQCNGLPPYFIEKDGMISDCDCRAIRMKINRIKTIYAKSGIDKKYQWRFFNSFEA
                     NSKLAANAKNIAYDLVSKFPDVRKGLFLWGNPGTGKTLLSSIILTELIIRHAIEGRFI
                     KISRTFFNKIRATFSEGSATYGTAQEIEYELQNADILVVDDFGVQRDSAWEQETLYNL
                     VDARYEAEKFTLFTSNVDPYRALADIAEGRVLSRIKEMCTIIELTGSDYRENL"
     gene            1369..1830
                     /gene="folK"
                     /locus_tag="PLJ75_13195"
     CDS             1369..1830
                     /gene="folK"
                     /locus_tag="PLJ75_13195"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPD06212.1"
                     /translation="METIAYIVLGSNIDDRLANIHQALSLLSTVEDTTIVKQSSIIET
                     QPLEFTEQPDFLNCVVEIVTGLKAENVLHHLLDIEDKLGRVRTVAKGPRIIDCDLLLF
                     GNVVCTTKHLTLPHPQIYSRPFVWQLLSEINMNIIDPVSHKLLSEVVACHQ"
     gene            1818..>2247
                     /locus_tag="PLJ75_13200"
     CDS             1818..>2247
                     /locus_tag="PLJ75_13200"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006039225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPD06213.1"
                     /translation="MSSIKNIKDFAKMKQAGQPITMITCYDYAFARIVNETDIDTILV
                     GDSLGNVVAGYDTTIPVTLDQMIYHSSMVKRGAPGSFIVTDLPFMSYHVSIADTMRNC
                     GRIMKETGSNAVKLEGGKDFVPVVEALVKASIPVMGHLGLT"
BASE COUNT          709 a          452 c          438 g          648 t
ORIGIN      
        1 ataatctcac cacgttagaa ccttttacgt atacccttgc acccattgat gagttgcaaa
       61 cctgccgatg cctcagcgtt acaaagcctt tacgaacaca ggatgatcgc ctatcataca
      121 gcaccattgc agatattgtt gatatggagc tggcaggaat tgcatatgca tcatataaat
      181 ttggaaaaca atgctattcc ataaaatata taagcgatac aggacaacac acaacacacg
      241 aaacaataat gaataacatt atcaattttt cagaaaaaac agttgataca attctggaga
      301 tactgttttc tcatgcatta atcaaataaa tcacaatcat tatgataata actcatttgc
      361 cattttttca actttctttg cattatgtac cagcatatct gctaaactta ctgtattttg
      421 aattgattca tttatgctga atatttactg gtgcaatttt cccagcaaca aaatatttga
      481 aattgtcatt gaaaatatat agactgtttc tatagtatat caattgtagc aattatactt
      541 cttcggagtg gcgatgcaga caaaaactaa aacgacttct cagcacaata ctttttgctc
      601 gtattgtgaa tatacgggga ttgttcgcaa tccaaacttt gaacagatag gtgaagagcc
      661 gctggtcccc tgcccgcggt gtgtgttgac aaaatgccaa tgcaatggat taccccccta
      721 ttttattgaa aaagacggca tgatatccga ttgcgattgc cgtgcaatac gtatgaagat
      781 caatcgcatt aaaacaatct atgcaaagtc aggtatcgat aaaaagtacc aatggcgctt
      841 tttcaactca tttgaagcaa actctaaatt agcagcaaat gcaaaaaata tagcatatga
      901 tttagtttca aaatttcctg atgtacggaa gggattattt ttatggggta atcccggcac
      961 cggaaaaacc cttctttcgt ccattatcct tacagagctt atcatccgcc atgcaattga
     1021 aggtagattt ataaaaatat ccaggacatt tttcaataaa atccgcgcaa cattttcaga
     1081 gggctctgcc acctatggga cagctcagga gattgaatat gaacttcaaa atgctgacat
     1141 acttgtggtt gatgattttg gcgttcagcg cgattcagca tgggagcagg aaacattgta
     1201 taatctggtt gatgctcgct atgaagcaga aaaatttaca ctattcactt caaatgttga
     1261 tccataccgg gcgctggctg atatcgcaga aggcagggtt ctttcccgca ttaaggagat
     1321 gtgtactatt atagaattaa caggtagtga ttacagggaa aacctataat ggaaactatc
     1381 gcttatatag tattgggaag caacatagat gacaggcttg caaacataca ccaggcactt
     1441 tcattgttat caacagttga ggatacaacg atagtgaagc aaagcagcat tattgaaacc
     1501 caaccccttg aatttaccga gcagccagac tttttaaact gcgttgttga gatagttaca
     1561 gggcttaaag ctgaaaatgt attacaccat ttgctggata tagaagataa gcttggcagg
     1621 gtacgtactg tggcaaaagg gccccgcatc atcgactgtg atctgctgct ctttggcaat
     1681 gttgtatgca ctaccaaaca tttaacactt cctcatccac agatatattc gcgtccgttt
     1741 gtgtggcagc ttttatcaga aataaatatg aacatcattg atcctgtttc acataaactt
     1801 ttaagcgagg ttgtagcatg tcatcaataa aaaacatcaa agactttgca aagatgaaac
     1861 aagccgggca accaattacc atgataacct gttacgatta tgcatttgca cgtattgtaa
     1921 acgaaactga tatcgataca atactggttg gcgattcact gggcaatgtt gttgccggat
     1981 acgacacaac tattccagtg acgcttgacc agatgatata ccatagcagt atggttaagc
     2041 gcggtgctcc cggcagcttt attgttactg accttccttt catgagctat cacgtttcca
     2101 tagcagatac catgcgcaat tgcgggcgca tcatgaagga aaccgggagc aatgcggtta
     2161 agcttgaagg cggcaaggat tttgtccctg tagtggaagc gcttgtcaaa gcttctatcc
     2221 ccgtcatggg acatcttggt ttaacac
//
LOCUS       DAOOJH010000213         2166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_40044_length_2166_cov_8.927996, whole genome shotgun sequence.
ACCESSION   DAOOJH010000213 DAOOJH010000000
VERSION     DAOOJH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40044_length_2166_cov_8.927996"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="PLJ75_13205"
     CDS             <1..1829
                     /locus_tag="PLJ75_13205"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPD06214.1"
                     /translation="GQMIKDKRFIERGKYMVYNSGIRRTPHTGAANIALHAQFYREEL
                     KNCQIRNACDELNTLMAHFGIHDNELSHVVDQETYIVPVNITYFPIRAHRNIINKIAS
                     TFVKSLPERIEEELEVEGTMLVEGVDIDINFGNPIAISPYLKSYLAKRKIKSKNLYLQ
                     PQDLQNDVVLRKIAIDIMYRYMHDIYTMTTINHDHIFSYLLTRATNKIKEGDFKTKAY
                     CAIAALKKLELPSCHSALKLKQGYLLTDDPHGRYNDFIDAAKSEGLITVNNGIIYKNK
                     QKFSRAYEFHTIRKDNIVEVLKNEIEPLRNLTRRLQILYWTPMVCVRRSIAQSFYQRD
                     VQIFENDYKHYFKQGETKPKYIGMPFFKKRLFARAGVVLVHGYMAAPAEMRELADFLY
                     NQGYTVYCARLRGHGTSPEDLATRSYDEWYESVNRGYIVVKNLVKKVFICGFSTGAGL
                     ALLHAANKKDALCGAVAISAPYRLKNIASGLAPAVDTWNKFLSFLKIKKIGRLDFIPN
                     HPDHPDINYLKNPVSGIHQMETLMDVVEKRLNDIYIPVLIMQGAGDTTVDPRGAFELF
                     MKIPSEKKECTMVHSKSHGITRGDVGIVVARRVGAFLHDYRV"
     gene            complement(1834..>2166)
                     /locus_tag="PLJ75_13210"
     CDS             complement(1834..>2166)
                     /locus_tag="PLJ75_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06215.1"
                     /translation="VKNNPILLLKGGTQYTINGSLQIMSGIAFFHLFNNAANSNSKGR
                     GKYNFLLMTLGCNYMYTPDIVITGLLIVSRNNIDTYYHSDDSISLESETKSNVWTITV
                     GAGISYKL"
BASE COUNT          700 a          372 c          457 g          637 t
ORIGIN      
        1 aagggcagat gataaaggat aaaaggttta tagaacgtgg caaatatatg gtttacaact
       61 ccgggatacg acggacacct cataccggtg ctgccaatat tgcattacac gcacaatttt
      121 atagagaaga gttgaaaaac tgccaaattc gtaatgcatg tgatgagttg aacacattaa
      181 tggctcattt tggtatacat gataatgaat tatcacatgt tgttgatcag gaaacttata
      241 ttgtaccggt aaatataaca tattttccca tacgagcgca tcgaaatatt atcaataaga
      301 tagccagcac atttgttaaa agtttgcccg agcgcataga agaagagctt gaggttgaag
      361 ggacaatgct ggtagaaggt gttgatatcg atatcaattt tggcaatccc attgccataa
      421 gcccctattt aaagagctat ctggcaaaga ggaaaatcaa aagtaaaaac ctttacctgc
      481 aaccacagga tttgcaaaac gatgtagtgc tacggaaaat tgccattgat attatgtatc
      541 gatatatgca tgacatttat acaatgacaa ccatcaacca tgatcatatc ttttcgtatt
      601 tattgacgag agctacaaac aaaataaaag aaggtgattt taaaacaaag gcatattgtg
      661 caattgcggc attaaaaaaa ttagaattac catcgtgcca tagcgcatta aaattgaaac
      721 agggctatct tttaaccgat gatcctcatg gacgatataa cgattttatt gatgcagcta
      781 aatcagaagg acttataact gtcaacaatg gcattattta taaaaataaa caaaagtttt
      841 cccgggcata tgagttccat acaatacgga aggacaacat tgttgaggtt ttaaaaaatg
      901 aaatagaacc gttgcggaat ctgacgcgca ggttacaaat tttgtattgg accccaatgg
      961 tgtgcgtgcg gcgaagtatt gcacaatcgt tttatcagcg cgatgtgcag atatttgaaa
     1021 atgactataa gcactatttt aaacagggcg aaacaaaacc aaagtatatt ggtatgccgt
     1081 tttttaaaaa gaggctgttt gcaagagcag gggtggtgct ggtacatggt tatatggctg
     1141 cacctgcaga gatgcgtgag cttgctgatt ttttatataa tcagggatac acggtatact
     1201 gtgcacggct tagagggcat ggcacatcac cagaggatct tgcaacaagg agctatgacg
     1261 aatggtatga atcagtaaac cgcggctata ttgttgtaaa aaatttagtt aaaaaggtat
     1321 ttatctgtgg tttttctacc ggtgccgggc ttgcgctgct gcatgctgct aataaaaaag
     1381 atgctctatg cggcgctgtg gcaatcagtg caccgtatag attaaagaat attgcttcag
     1441 gtcttgctcc tgcagtagat acatggaata aatttttatc atttttaaaa ataaaaaaga
     1501 ttggcaggct ggattttatc cccaatcatc ctgatcatcc tgatattaac tatcttaaaa
     1561 atcccgtttc gggcattcat cagatggaaa cattgatgga tgttgttgag aaacggctta
     1621 acgacattta tatcccggta ttaataatgc agggtgctgg cgatacaacg gttgacccca
     1681 ggggtgcttt tgagctgttc atgaaaatac cctcagaaaa aaaagaatgt accatggttc
     1741 actcaaaaag ccatggtata acccgcggtg atgtgggtat tgttgttgca cggagagttg
     1801 gagctttttt gcatgattac agggtatagc acattataat ttatacgata tgcctgcacc
     1861 tactgtaatg gtccacacat tggattttgt ttctgattcc agcgaaatgc tgtcatctga
     1921 atgataatat gtatcaatgt tgttacgtga cacgataagc aagcctgtaa ttacgatatc
     1981 aggcgtatac atatagttgc atcccaatgt cattaataaa aaattatatt ttccccttcc
     2041 cttactgttg ctgttggcag cattattaaa aagatgaaaa aatgctatgc cactcataat
     2101 ctgcagtgat ccattgatgg tatattgggt gccgcctttt aatagtagta ttggattgtt
     2161 cttcac
//
LOCUS       DAOOJH010000214         2124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_41171_length_2124_cov_7.074915, whole genome shotgun sequence.
ACCESSION   DAOOJH010000214 DAOOJH010000000
VERSION     DAOOJH010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41171_length_2124_cov_7.074915"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLJ75_13215"
     CDS             complement(<1..938)
                     /locus_tag="PLJ75_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPD06216.1"
                     /translation="MLKINTCFISLLLFLSIAPAIYAQDNGSTQRNLTGIIETAAPST
                     NAQTGEIALNDVIISAYKSNITYAESASATVITQKDIEKGGYRFVSDVLQSVPGLQLV
                     QSSTLGGTASVFIRGAKTGNMVVLIDGVKVNDVSTIERTFDFATLATDSIERIEIIKG
                     PQSALYGSDATGGIINIVTKKGKGKPAVTLNMYAGSYYTTQLSANVSGGDSSWNYSLT
                     AGQISAQGYSKAAKPKTATTPFDDDGFYQRYAMGKCGFTPVAPLSIDAGFTVKKSAIE
                     VDDSSYTDDPDSDEERTEVSGYTTIKHTIASFWEQSV"
     regulatory      complement(1080..1215)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1338..1754
                     /locus_tag="PLJ75_13220"
                     /pseudo
     CDS             1338..1754
                     /locus_tag="PLJ75_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017297098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
BASE COUNT          662 a          372 c          438 g          652 t
ORIGIN      
        1 acgctttgtt cccagaacga tgcgatggta tgcttgatgg ttgtgtagcc tgaaacttcg
       61 gtacgctctt catcgctgtc aggatcatca gtatacgaag agtcatcaac ctcaatagct
      121 gactttttta cggtaaatcc agcatcaatt gataatgggg ctacgggggt aaatccacat
      181 tttcccatgg cgtaccgctg gtaaaagcca tcatcgtcaa aaggtgttgt agcagttttt
      241 ggctttgctg cttttgagta gccctgtgct gatatctgcc ctgcggtcaa agaataattc
      301 catgagctat cgccaccgct tacatttgcc gatagttgcg ttgtgtagta tgaaccggca
      361 tacatattca gtgttaccgc tggtttgcct ttcccctttt ttgtaacaat gttgataatc
      421 ccaccggttg catccgaacc atacagagcg ctttgcggac ctttaataat ttcaatgcgc
      481 tcaatagagt cggttgcaag ggtagcaaag tcaaatgttc gctcgatagt tgatacgtca
      541 ttaaccttta cgccatcaat caataccacc atgttgccgg tctttgcacc gcgaataaaa
      601 acgcttgcag taccacccag tgttgatgac tgaacaagct gcagccccgg caccgactgt
      661 aaaacatctg aaacaaaacg ataccctccc ttttcaatat ccttttgtgt tattacagta
      721 gcagaggcgc tttcagcata ggtgatgttg cttttgtaag cactgatgat aacatcgttt
      781 aatgcaatct cgcctgtctg tgcatttgtg gaaggggcgg ccgtctcaat aatgcccgtc
      841 agattacgtt gagttgaacc gttgtcctgt gcatatatgg caggagctat cgacagaaat
      901 aataaaagtg aaataaaaca agtgttgatt ttaagcattg aaacctcctc acagtttaaa
      961 agtgtatgtc tggaatgagg aggagggaaa ccgatacgct aaaaataaaa aagcatgccc
     1021 gaggcatgct acattagtta gaatcaaatt gctttgatac taactggttc ccaatcctcg
     1081 gggaattcca gctaaaggag tatctggctt gcccaccata tagtgggctc acagttgcgg
     1141 gtcagcgtgg gatttgcacc cagcttcctc cttgtctact gattttccag cagaatgatt
     1201 ctcattgatt aatggttact ataatctgtc aataattttt atacttactc ttactatatt
     1261 ttacgattga cttgtgatgg ggtggtatgt atggtattgt tgtctggtag attatccatt
     1321 gcaacataca atataatatg gtgggtatat ctatgcatga tgtgcggtat aaacaacgat
     1381 tttaaaatta tgaaaattca tttcttttgt taaaagcatc cattatgatt gaaaaaccat
     1441 caattattga aaaagccgga attattcagt tttttgaagt aacatttgaa ttgtcatgga
     1501 aactgctgaa agattactgt gaatttttag gatttactgt taattcacca cgcgaagcaa
     1561 taaaaagagc attaagtatt gatatgatat cgaatggaga aatatggtta agagcttgaa
     1621 tggataggaa tttgactgta catgtttatg atgaagcaat agcaaatgag gtgtatgaaa
     1681 agattaaaga tgagtatttt cctttattgg aacaaccata taaaaaatgt aaggacgaac
     1741 tatgtatgga ttaaatgaca aagaattaca acaaataaaa gatgtagttg tatcctgtga
     1801 tgtcacacga tgtattttat ttgggtctta tgcaaaggga tgtgcaaaaa agggcagagg
     1861 ctctgacccc ttatatttat ataaattcta tagcaaaaaa taaatgtact gttattacgc
     1921 caattgaaag tgtaataata tattaatgtg ttgactcatg aataaagttt tattatcctg
     1981 ttaaaagagc gtccgctcat attgtaaatt ttgtaaatta ttgtcttaaa agataaagaa
     2041 aatcacacaa tggaagatag gtagtatgaa tagcattgaa agtataacat cagaaggaag
     2101 aaaaatagcc aataaaatca ggat
//
LOCUS       DAOOJH010000215         2121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_41255_length_2121_cov_3.775895, whole genome shotgun sequence.
ACCESSION   DAOOJH010000215 DAOOJH010000000
VERSION     DAOOJH010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41255_length_2121_cov_3.775895"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PLJ75_13225"
     CDS             <1..1333
                     /locus_tag="PLJ75_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPD06217.1"
                     /translation="KTIVDALTAEIVPAVLATRFYCKNAQKFLNDRKLPMGSILFVNK
                     RSKIVRVPYGVVGIISPWNYPFSIPYSEIIMGLLAGNAVIVKTASETQCVGHILKECI
                     EAAELPPHIFTYINMPGSIAGDAFIEAGIDKLFFTGSVRVGKYLMNKASERLTPLVLE
                     LGGNDPMIVCDDADLYRAAAGAVWAGMQNAGQSCGGVERIYVDRKVYNKFLTILKGMV
                     ESLRVGDGSDISSDIGAMTTKRQMEVVQHHIDDAVKKGATIYAKSKVNSKKGQFMPCM
                     VLTDVNHDMLTMKDETFGPIVGVMPYDTIDEAIALSNDSYLGLTASVWSKNRSKAMKI
                     GRQIKAGAITINDHLMSHGLAETPWGGFKESGIGRTHGDIGFAEMTQPQVIVNDILPF
                     ARRNFWWKPSDQRQYEGLKGAIDVLYAKRVSHKMKALFNLSKAFLTTFIRL"
     gene            1636..>2121
                     /locus_tag="PLJ75_13230"
     CDS             1636..>2121
                     /locus_tag="PLJ75_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06218.1"
                     /translation="MDNNFNSKDILDDSDTDLEVMLGGMGKSLLSKFPIIGVLIEGYD
                     SYKSYQNDRLIKKALKNLSDRIEEIEKICHDDWIKTQDGKIFVYKIMDCILDSQLEDK
                     QEYFINTLINGINDKQLSTLDKYNFIDILRKLSKNSLIILSELYNLYKDVVRIPGREI
                     IG"
BASE COUNT          726 a          277 c          458 g          660 t
ORIGIN      
        1 taaaacaata gttgatgcat tgactgctga gatagttcct gctgtgcttg ctacaaggtt
       61 ttattgtaaa aatgcacaaa aattcctgaa cgatagaaag ctacccatgg gttctatatt
      121 gtttgtcaat aagcgaagta aaatagtgcg tgttccctat ggtgttgtag gtattatatc
      181 cccatggaat tatccatttt ccattcctta ttctgaaatt atcatgggat tgttagcagg
      241 gaatgcagta atagtaaaaa cagcttcaga aacacaatgt gtaggtcata ttttaaaaga
      301 gtgtattgaa gcagctgaat taccccctca tatatttacc tatatcaata tgccgggtag
      361 tattgctggt gatgcattca ttgaggctgg tatagataaa ttgtttttta ctggttcggt
      421 aagggtgggc aaatacctta tgaataaagc atctgaacga ttaactcctc tggtacttga
      481 acttggtggc aatgacccaa tgattgtttg tgatgatgcc gatctgtata gagctgctgc
      541 aggtgctgta tgggcaggta tgcaaaatgc agggcagtca tgtggagggg ttgaaaggat
      601 atatgtcgat agaaaagtat ataataaatt ccttacaatc ttaaagggca tggtagaatc
      661 attgcgagtt ggtgatggga gtgatatctc atccgatatt ggtgcgatga caacaaagag
      721 gcagatggag gtagtacaac atcatattga cgatgctgtt aaaaaaggtg caacaatata
      781 tgcaaaatca aaggttaata gtaaaaaagg ccagtttatg ccatgtatgg tacttacgga
      841 tgttaaccat gatatgctga caatgaaaga tgaaaccttt ggacccatag taggcgttat
      901 gccgtatgat acaattgatg aggcaatagc gttgtcaaat gattcgtatc ttggattaac
      961 agcgtcagta tggtcaaaga acagatctaa agcaatgaag attggcaggc aaataaaagc
     1021 aggtgccatc acaataaatg atcatttaat gagccatgga ttggctgaaa caccgtgggg
     1081 tggatttaaa gaaagcggga taggaagaac tcatggtgat attggttttg cagaaatgac
     1141 acagccacag gtcattgtaa atgatatttt accatttgca cgccgaaatt tctggtggaa
     1201 gccttctgat caaagacaat atgaaggact aaagggagca attgatgttc tttatgctaa
     1261 aagggtttcc cataaaatga aggcattgtt taatttatca aaagcttttt tgacaacatt
     1321 tatcaggcta tagttcgtaa cttatatttc gataaattac catgcgtttt cagtgtacgt
     1381 gatgttttgt gaagagtcaa tggtatagct aaataattgc attaagcatg aaacatagaa
     1441 tcatcgttat ggtgagtggt gtaaagatga tttatggtta tgctgtaacc acccataggg
     1501 gataaacata caacgctact gtcattctga gccggaggca aagaatctcg ttgttgcatg
     1561 aagggattct ttagttgtta tactctctta gaatgataat agtaaataat tcagtttttg
     1621 ggtgagtaac aaattatgga taacaatttc aattcaaaag atattttaga tgatagtgat
     1681 actgatcttg aagtaatgtt gggaggaatg ggtaaatcat tgctttcaaa atttcctatt
     1741 attggagtac ttattgaagg ctatgattct tataagtcat accaaaatga tagactgata
     1801 aagaaagctt tgaaaaatct atctgataga attgaagaaa ttgaaaaaat ttgtcatgat
     1861 gattggatta agacacagga tggaaaaata tttgtatata agataatgga ttgcatactg
     1921 gattcacagt tagaagataa acaggaatat tttataaata cattaataaa tggaattaat
     1981 gataaacaat tatcaacatt agacaaatat aattttatag atatattgag aaaattaagt
     2041 aaaaattctt taatcatatt atcagaatta tataatttgt ataaggatgt tgttagaatt
     2101 cctggtagag aaattattgg g
//
LOCUS       DAOOJH010000216         1918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_47799_length_1918_cov_7.425658, whole genome shotgun sequence.
ACCESSION   DAOOJH010000216 DAOOJH010000000
VERSION     DAOOJH010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47799_length_1918_cov_7.425658"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1061
                     /gene="sppA"
                     /locus_tag="PLJ75_13235"
     CDS             132..1061
                     /gene="sppA"
                     /locus_tag="PLJ75_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPD06219.1"
                     /translation="MDKNRRILIAILAMVVFAVVLAIIQISITMQRQMTIGTRINLTE
                     GPGVGIVRVYGRIEATGDMMAQANADYIVKRLDALAQDSRIRAIVIRINSPGGTVAAT
                     QEIYTKILSLRKKNIPIIASMGDVAASGGYYIASACNAIVVNYGTITGSIGVIAMAPN
                     LKGLFDKLGIRMNVIKSGRYKDILAMHREISQEELQLIQQIIDSSYQKFVHDVAAGRN
                     MAVSDIMPYADGRVFTGNQAVEYKLADELGSYDDAVNKARTLAKLPVDSAVYDDTASP
                     IERLLMTMSSFFKPKLEQMVNSQYSLVEYSYMP"
     gene            1064..1645
                     /locus_tag="PLJ75_13240"
     CDS             1064..1645
                     /locus_tag="PLJ75_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06220.1"
                     /translation="MIQILDYWYSALIDPEKLSQIIAVEDKKHFIVSAVVVAIVVFMD
                     IVAQSLLSTQTGFFYYKITYGFIALFLLHVLCLIIYAGLLTVILQIGGYQASGGYIIN
                     VLALSQIPMIFIVPVVLIFKSLYFAPVFFYSAASLALFIWSLFIAMRCISQRYQIETL
                     KTIGYIALPAILAGVTAFLILVAGGMVLIGLIK"
     gene            complement(1646..1834)
                     /gene="rpmB"
                     /locus_tag="PLJ75_13245"
     CDS             complement(1646..1834)
                     /gene="rpmB"
                     /locus_tag="PLJ75_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPD06221.1"
                     /translation="MAKCEVCGKSILFGHNVSHSNKKTGKIWHANVQKIRVVEGNKVV
                     RKNVCTRCIRSGAVRKAI"
BASE COUNT          533 a          349 c          445 g          591 t
ORIGIN      
        1 agagcaacgg ttttatacta taccgactta tcggttttaa tagtaactat cgccaaaaaa
       61 agcaagaaaa aaatgttgca tttattactg cccatggtgt attgttgccc aatatcaaca
      121 aggaataggg tatggacaaa aacagacgga tattgatagc catactggca atggtggttt
      181 ttgcggttgt acttgcaata atccagataa gcataaccat gcagcgccag atgaccattg
      241 gtacacggat taacctcacc gagggtcccg gagttggcat cgtgcgtgtg tatggacgta
      301 tcgaagcaac cggtgatatg atggcgcagg ctaatgccga ttacattgta aaaaggcttg
      361 atgcattagc acaggacagc cgcatcaggg cgatagttat acggattaat tctcctggcg
      421 ggacggttgc tgcaacacag gagatatata ccaagatact atcgcttaga aaaaaaaata
      481 ttcccatcat agcttcgatg ggtgatgtag cagcgtcagg cggttactac attgcttcgg
      541 cgtgcaatgc tattgttgtc aactatggca ccataacagg ctcaattggc gttattgcca
      601 tggctcccaa tctcaaaggc ttatttgata agttgggtat ccgtatgaat gtaataaaaa
      661 gcggaaggta taaggatatc cttgccatgc acagagagat ttcgcaggaa gaattgcagt
      721 tgattcaaca gattattgat tcaagttatc agaagtttgt gcacgatgta gcagcaggca
      781 gaaatatggc ggtaagcgat atcatgccgt atgctgatgg gcgtgtgttt accggcaatc
      841 aggcggttga gtataaactt gctgatgagc tgggcagcta tgacgatgca gtcaataagg
      901 cgcgtacatt ggcaaaactg ccggttgatt cagccgttta tgatgatacg gcaagcccca
      961 ttgaacggtt gctgatgacg atgtcatcgt tctttaaacc aaaattagag cagatggtaa
     1021 actcacagta ttctttagtg gaatatagtt acatgccatg aatgtgatac agatattgga
     1081 ttattggtat agtgcgctga ttgatcctga aaagctgtcg cagattatag cggttgagga
     1141 taaaaagcat ttcatcgtca gcgctgtggt tgtggcgata gttgtcttta tggatattgt
     1201 agcgcaatct ctgctttcaa cgcaaacagg atttttttac tataagatta cctatggttt
     1261 catagcgttg tttttacttc atgtattgtg ccttataata tacgcagggt tgttaacggt
     1321 aatattgcag ataggcggct atcaggcttc gggtggttat atcatcaatg tactggcgct
     1381 gtcgcagatt cccatgattt ttatagtacc tgttgtactg atattcaaat cattatattt
     1441 tgcgccggta tttttttaca gcgccgcatc gttagcactg tttatatggt cgttgtttat
     1501 tgcaatgcgt tgcatttccc agcgctatca gatagaaact ctaaagacaa tagggtatat
     1561 agctctccct gcaatactgg caggggtaac agcttttttg atactggtgg ctggtggtat
     1621 ggttttaatt ggtttaataa agtgattata tagccttgcg cactgctccc gagcggatac
     1681 agcgtgtgca tacgttttta cggactactt tgtttccctc taccacgcga attttttgaa
     1741 catttgcatg ccatattttg ccggtttttt tattggaatg gcttacatta tgaccaaaca
     1801 gtatagattt gccacaaact tcacatttag ccattggtat ctcccttact ggtaattatt
     1861 tactatataa tagcattttt aaaagcaaaa aaaatatttt tatgggcgat agttactg
//
LOCUS       DAOOJH010000217         1895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_48646_length_1895_cov_2.667391, whole genome shotgun sequence.
ACCESSION   DAOOJH010000217 DAOOJH010000000
VERSION     DAOOJH010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48646_length_1895_cov_2.667391"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(26..166)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            545..1399
                     /locus_tag="PLJ75_13250"
     CDS             545..1399
                     /locus_tag="PLJ75_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HPD06222.1"
                     /translation="MAINEQSLELSHKLLLQPLLQKVTIPISEYSFANLYLFRSVHQY
                     ILITGDITAIKGVTYDGIHYIMPVMPLDEEYAEKLVQLLQPNMCLFPIHHDWLHFFTE
                     KGFTYHYNDGDSDYVYTKEKLATYSGKKLHGKRNLLYQFTAKYSVEVLPLTSQRVNDA
                     LYILDIWKKESGNDADIAACIEACMLLDELNLCGSMYYVEGKPAAFIIGEAITDDMFA
                     IHFAKAITSYKGIYQYMYNHCAKALPANYKYFNFEQDLGLEPLRFAKRSYQPDMIVTK
                     YRVSALIQ"
     gene            1396..>1895
                     /locus_tag="PLJ75_13255"
     CDS             1396..>1895
                     /locus_tag="PLJ75_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPD06223.1"
                     /translation="MTIEQLIEYLKNYEPFMKQVAAWYTVPARQAVYAPYPDDIDERI
                     RNVYMQRGIENLYSHQRSAYDAVQKKENIVVVTPTASGKTLCYNLPVLNFMAKHPDAR
                     ALYLFPTKALSQDQLSELQEIMKITGLSIKTYTFDGDTPQSARKAIRTAGNIVITNPD
                     MLHSGIL"
BASE COUNT          618 a          372 c          330 g          575 t
ORIGIN      
        1 accgcaaaaa gcagttttat agtgctcttg cccttccaaa agcctacgag gttagctgac
       61 gggctcgggt ttggaagtac cctatcctta tgtaaaagga atacgcccca aaaattgggt
      121 cccccgcatc cattgctgct gtaatggatt taggcttttt tatgcagcat gtaaaattgg
      181 ctattaatat actttaaatt ctttatctcg tcaataataa atattatttt ttatatttat
      241 tcttaaaaga aattaattca attattaaag tgatgtagct atacacattt tgttataatg
      301 cgttatacgt aaccatgctt ttttattctc agcatcataa tttatgaaaa agcaacaatg
      361 atttcattac atcttttgca agctaagaga aattatcgtg tcacaaaact ccacatctca
      421 ccggtagctt ttaatattta acctcctgtg gattgttctt gtaaatttga aaaaaagaga
      481 ttgaaaaagt atatatacac aatacattgt tggtaaatat atatctatat aaaaaatggc
      541 tactatggct atcaatgaac aatcattaga actatcgcac aaattattac ttcagcctct
      601 tttgcaaaag gtaaccatac ctatatctga gtattcattt gcaaacctgt atctttttcg
      661 ttcagttcac cagtacatcc ttataacagg ggatatcaca gcaattaaag gtgtaacata
      721 tgacggtata cactatatta tgcctgttat gccgctggat gaagaatatg ctgaaaaact
      781 ggtacaactc ctgcaaccca atatgtgtct atttcccatt caccatgact ggttgcattt
      841 ttttactgaa aaaggcttta cctatcatta caatgatggc gactccgatt atgtctatac
      901 caaagaaaag ttagccacct attccgggaa aaaacttcat ggaaaaagaa atcttctgta
      961 tcagtttacg gcaaaatatt ctgtagaagt gcttccatta accagccaga gagttaatga
     1021 tgcattatat atacttgaca tatggaaaaa ggaatccggc aatgatgcgg atattgctgc
     1081 gtgcattgaa gcgtgtatgt tgcttgacga attaaatctg tgcggcagca tgtactacgt
     1141 tgaaggaaaa cctgctgcat tcatcattgg agaagcaata actgatgata tgtttgcaat
     1201 tcattttgca aaagctataa caagttacaa aggaatttat caatacatgt ataatcactg
     1261 tgcaaaagca ttgcccgcaa attataaata ttttaacttt gaacaggatt taggattaga
     1321 accgctgcgc tttgcaaagc gttcatatca gcccgatatg atagtgacaa aatatcgtgt
     1381 gagtgcttta atccaatgac tattgaacag cttattgaat atcttaaaaa ctatgaaccg
     1441 tttatgaaac aggttgcagc atggtatacg gtacctgcca ggcaagctgt ctatgcacca
     1501 tatccggatg acatagacga acgaatccgc aatgtataca tgcagcgtgg catagaaaat
     1561 ctttacagcc atcagcgctc agcatatgat gcagttcaaa aaaaagaaaa tattgtggta
     1621 gtaacgccaa cagcttcggg caaaacgcta tgctacaatc ttcccgtgct taattttatg
     1681 gccaaacatc ccgacgcacg ggcgctgtac cttttcccca caaaagccct gtcgcaggat
     1741 cagctttctg aacttcaaga gattatgaag atcaccggtt tatccattaa aacatacacc
     1801 tttgacggtg atacaccaca gtcagctcgt aaagctattc gcacagcagg taatattgtc
     1861 atcaccaatc ccgatatgct gcattcaggt attct
//
LOCUS       DAOOJH010000218         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_48665_length_1894_cov_8.295269, whole genome shotgun sequence.
ACCESSION   DAOOJH010000218 DAOOJH010000000
VERSION     DAOOJH010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48665_length_1894_cov_8.295269"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..1085
                     /locus_tag="PLJ75_13260"
     CDS             402..1085
                     /locus_tag="PLJ75_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06224.1"
                     /translation="MATVSTIIVVVAGIIALFIVLTITNILQAGSKKAHTRSMADILG
                     VDPKKATYDDVEKLSRKETMQLFIAASTPDFKALNGEFKARLLSGGVLGKSSELFTHH
                     VFPTGWITVHTKWIGKAFKPASDSSGHGYNLFEEKTKSGTRILRLRKIDTNLAPSKIA
                     RDNKNSFQINYSRYNKGTVHSMRDEIRKINDNLYIGMGYMGPGGGSANPAPFALIGKP
                     SPWVGPDKS"
     gene            complement(1088..>1894)
                     /locus_tag="PLJ75_13265"
     CDS             complement(1088..>1894)
                     /locus_tag="PLJ75_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPD06225.1"
                     /translation="LKGELALVTGAAMGMGRSVSQLLLEEGCDVAMVDINGKELTKNA
                     KQLKAKGNCKAYICDVSKRDAVYRLQKKVKQEMGDVTILVNNAGVVRVGDFLHLDDKA
                     IDWIVDVNLKSIFWMCKAFMPGMITKKWAHVVNVASAGGLLALPDISIYCATKFGVVG
                     FSDALRQEMKKYKYNVGVTMVCPYAVATGMFEGFKEVAMTKILKTEDVTVKVVKAIKK
                     NRAMVAVPNFQVNFLTPLTKLILPVHAMDIINKLAGMWTANEHTTGRRGK"
BASE COUNT          583 a          389 c          298 g          624 t
ORIGIN      
        1 gttaaataat cgctgtcttg atatttttca aataatcctc cagaaaactt cataattata
       61 cctctgtaat cgtatattat tgtattatta ctatttttat aatttgtata tgcttttatg
      121 tccttagcaa tgcgaggaat ttagatacca ttactgtcaa tttgtcaaga ataatttatc
      181 gttaacaata aaaatgaatg tgccaatata atatatgatt gactgtccaa ccaatattat
      241 tgttgacaat tcttatgctg tgatgtatcg taatgattag acaatttcat atcagacact
      301 ttgccattgc caatgtatca aaaccatgtg ttctgagttt tataccctgt tgcaaagcaa
      361 tttttcatat gttctaaatg aactattgat ggaggtttat aatggcaacg gtatctacca
      421 ttatagttgt tgtagctggt atcatagcat tgtttatagt gttaactatc acaaacattt
      481 tacaggcagg cagcaaaaaa gctcatacaa ggtctatggc tgatatactt ggagtagacc
      541 ctaaaaaagc cacctatgat gatgttgaaa agctatcccg caaggaaaca atgcagcttt
      601 tcattgcggc atcaacccct gattttaaag cactcaatgg tgaatttaaa gcacgcttgt
      661 tgagtggtgg cgttttaggc aaatcatctg aactttttac ccatcatgtg ttcccaacag
      721 gatggataac agtacataca aaatggattg gaaaggcttt taaacctgca agcgatagtt
      781 ccggtcatgg gtacaattta tttgaagaaa aaaccaaatc aggaaccagg atactcagat
      841 taagaaagat cgatactaac cttgctcctt caaaaattgc cagagataat aaaaattcgt
      901 ttcagattaa ttacagcaga tataataaag gcaccgttca ttccatgcgc gatgaaatac
      961 gcaagattaa cgacaatctt tacattggca tgggatatat gggaccggga ggtggttcgg
     1021 caaaccctgc accatttgct ctgattggta aaccttcacc atgggttggc cctgataaat
     1081 cttaagctta tttgccccgc cttcccgtag tatgttcatt ggcagtccac attcctgcta
     1141 atttattgat tatgtccatt gcatgaacag gcaatatcaa tttagtaaga ggcgttaaaa
     1201 aatttacctg gaagttagga acagcaacca ttgccctgtt cttttttata gcttttacta
     1261 cctttactgt tacatcttct gtttttaaaa ttttagtcat agcaacttct ttaaatcctt
     1321 caaacatacc ggttgccact gcataagggc ataccatagt tactcccaca ttgtatttat
     1381 attttttcat ctcctgtcgt aatgcatctg aaaaacctac aaccccaaat tttgtagcac
     1441 aatatattga tatatccggt agcgctaaca gccctccagc cgatgcaaca ttgacaacat
     1501 gagcccattt tttagttatc attcctggca taaatgcttt gcacatccag aagatagatt
     1561 tcaaattaac atcaacaatc caatcaattg ctttatcatc cagatgcaaa aagtcgccta
     1621 cccttactac tccagcatta tttacaagaa ttgttacgtc gcccatttct tgtttaactt
     1681 ttttttgtaa ccggtacaca gcatcacgtt ttgatacatc acatatataa gctttgcaat
     1741 tacccttagc cttcaattgt ttagcatttt ttgttaattc ttttccattg atatcaacca
     1801 ttgcaacatc acatccctct tccagtaaaa gctgggacac acttctgccc attcccatag
     1861 ctgctccagt taccagtgct aattcacctt tcaa
//
LOCUS       DAOOJH010000219         1889 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_48849_length_1889_cov_8.135224, whole genome shotgun sequence.
ACCESSION   DAOOJH010000219 DAOOJH010000000
VERSION     DAOOJH010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48849_length_1889_cov_8.135224"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..689
                     /locus_tag="PLJ75_13270"
     CDS             33..689
                     /locus_tag="PLJ75_13270"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPD06226.1"
                     /translation="MGEKPDFALEVQEYKKGCTIIGGIDEAGRGSLAGPLAVCMVIYP
                     CHLFVNPPGDVIGIVNDSKKLSHKKRLAAKEIIIAHCLWWKCVLIPHNIIDEKNINGA
                     TYYAIVQLLQNNTMTPDMIFIDGNFSFPLPIPYRSIIKGDNLSLTIASASIIAKVTRD
                     EFMDKLDADYPAYGFAHHKGYGTREHVQAIYHHGPSPVHRKTYQPVAGLLAGGSTLSG
                     "
     gene            686..1660
                     /locus_tag="PLJ75_13275"
     CDS             686..1660
                     /locus_tag="PLJ75_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HPD06227.1"
                     /translation="MKITNNIVSAGLTQTSSLTLKSGQVVVGTITRRMDTTTAMLRVG
                     SQLFETSFAKGLPKSNHILLRYDTMQGNRFIFTMVEPETMKHNDILLATTSNYRAFLH
                     SLNTLDDITVFSIHKALMASDLLRPLRFTSIAPLLQRLLQRGLSKQTLQKFCYTISKD
                     SGISVIMQLLHNAGNNVLNQLELSDEVIQDVFSMMSKEDIESLLYYASDSSRSSYTIP
                     FYDSNGFNDAYILADKDFVCIDVTFEHLGKLNISISKQKGLSIKIYCFNDGAYNALQP
                     AMQQLSKTIEEATGETAQCNCILRKDWEEKIIALINSMYIHYSIDFSV"
     gene            1688..>1889
                     /locus_tag="PLJ75_13280"
     CDS             1688..>1889
                     /locus_tag="PLJ75_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HPD06228.1"
                     /translation="MEDIAVALEYLTEDKLPKIIARGKGRLAKTILDRAKEHNIPVYQ
                     DTDLATVLLTLESSYIPEELYKA"
BASE COUNT          631 a          355 c          360 g          543 t
ORIGIN      
        1 acaaaatcta atgtataaat aatagttata ctatgggtga aaaacctgat tttgcattag
       61 aagttcagga atataaaaaa ggctgtacaa taataggtgg cattgatgaa gcgggaaggg
      121 gctctttagc ggggcctctt gctgtttgca tggttattta tccctgccat ctttttgtta
      181 acccaccggg tgatgttata ggtattgtca atgattcaaa aaaattatct cataaaaaaa
      241 gattggctgc gaaagaaata atcatagcac attgcctgtg gtggaaatgt gttctaatac
      301 ctcacaatat aatagatgaa aagaatatca atggagcaac atattacgcc attgtacaac
      361 ttttacaaaa caataccatg acacctgata tgattttcat agatggcaat ttttcgtttc
      421 cactgcctat accatatcga tcaattataa aaggagataa cctttcgctt accattgcat
      481 cagcatcaat cattgccaag gtaactcgtg acgaattcat ggataagctt gatgctgact
      541 atcctgcata cggttttgca catcacaagg gatatggcac ccgtgaacat gtacaggcta
      601 tatatcacca tgggccatcc cctgttcacc gcaaaactta tcaacctgtt gcaggattac
      661 ttgcaggagg atcaacactc tctggatgaa aattaccaat aacattgtca gtgcaggatt
      721 aacacaaacc tcatcactga cgctgaaaag cggacaggtt gttgttggca caataaccag
      781 acgaatggat actacaacag cgatgttgcg tgtaggaagc cagctttttg aaacaagctt
      841 tgcaaaggga ttgccaaagt ccaaccacat actactgcga tatgacacaa tgcagggcaa
      901 ccgatttata tttactatgg tagagcctga aacaatgaaa cacaatgata tattgcttgc
      961 aaccacctca aactacagag catttttgca ttctctcaac acacttgacg atattactgt
     1021 tttttcaatc cataaagctc tgatggcaag cgacctgctg cgtccgcttc gttttacctc
     1081 gattgcgccc ctgctgcagc gtttattgca gagaggattg tcaaaacaaa cactgcaaaa
     1141 attttgttac actatttcaa aagattctgg tattagtgta attatgcagt tactgcacaa
     1201 tgcgggcaat aatgtgctca atcaactgga acttagtgat gaagtaatac aggatgtttt
     1261 ttcaatgatg tcaaaagagg atattgaaag tttactctat tatgcaagcg atagttcaag
     1321 aagcagctat accatcccct tttatgacag caatggattt aatgatgcat atatccttgc
     1381 cgataaggat tttgtatgca tagatgtaac ctttgaacat cttggtaaac ttaacattag
     1441 catcagtaaa caaaaggggc tttctataaa aatctattgt tttaatgatg gtgcgtataa
     1501 cgctttacag cctgctatgc aacaactatc gaaaacaata gaagaagcta ccggtgaaac
     1561 tgcacaatgc aattgtatat tgcgcaaaga ctgggaagaa aaaataattg cattaattaa
     1621 cagtatgtat atacattata gcattgattt ttctgtatag agtaaatgta atttcaggaa
     1681 aaatatcatg gaagatattg cggtagcact ggaatactta accgaagaca agctgcctaa
     1741 aattatagcc cgggggaaag gcagattggc taaaactatt cttgacagag ctaaagaaca
     1801 taatatacct gtttatcagg atactgattt agcaacggtc cttttgaccc ttgaaagttc
     1861 atatattcct gaagagctct ataaagcag
//
LOCUS       DAOOJH010000220         1791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_52904_length_1791_cov_7.513249, whole genome shotgun sequence.
ACCESSION   DAOOJH010000220 DAOOJH010000000
VERSION     DAOOJH010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52904_length_1791_cov_7.513249"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PLJ75_13285"
     CDS             <1..206
                     /locus_tag="PLJ75_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPD06229.1"
                     /translation="DFRHSFHTIYWLIRCVVGLHLEDQPMTRHYRNFLTRTLFSPFMQ
                     KVERFFDNFFPKSIILYAQKPLQ"
     gene            268..1224
                     /locus_tag="PLJ75_13290"
     CDS             268..1224
                     /locus_tag="PLJ75_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPD06230.1"
                     /translation="MALTPTSVRTLQHYLEEAIFVGQAHKLGVFRQLGNKPDTASALA
                     RRMQFDTRATWILLEALVEMGYLQKDNDIYSPTQYCIDHLVNEESDDYDGDFLKFLLY
                     LMHPWQTLPYVLKTGTPDESSFKELSMQDFIKGMDSPWKKKITPEVVSLCLSYCTASS
                     CIDIGGAPGTIAREFVKRGVKTIIFDLPQSLAITQDALKDIPDIEIIAGDATVALPHC
                     KVDIAFLGNLCHGQSPQDNQKIISMCYDIVNPDGIIAIFDNLRDESYLGATLALHMLT
                     QSKHGNIYAREEYFAWLHNAGFKEPQVLQLSDKAWQMIIAKK"
     gene            complement(1227..1757)
                     /locus_tag="PLJ75_13295"
     CDS             complement(1227..1757)
                     /locus_tag="PLJ75_13295"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPD06231.1"
                     /translation="MELRTVIRHILGYAVGFTIFLVCLPAVIYFIASYFHQLIPAGII
                     GNTIIRVLVSVVLFLIGLIFMIWSNVALLVIGKGGPVDAFNVSISPRTKHLVVKGLYR
                     YTRNPMVFGALCCYIAFALYLNSLVVVVFILAFVPVIIIYLKQTEEKRLEQDFGQEFR
                     EYSKKVSMLLPLPPHK"
BASE COUNT          583 a          382 c          356 g          470 t
ORIGIN      
        1 ttgatttccg tcattcattc catactatct actggctcat ccgatgcgtg gttgggcttc
       61 atcttgagga ccagccaatg accaggcact atcgcaactt tttaacccgt accctttttt
      121 ctccttttat gcagaaggtg gagcgctttt ttgacaattt ttttcccaaa agtattatac
      181 tgtacgccca aaaaccatta caataattgt ggcgatagtt attgatgata tatgactgat
      241 taataaaaat taataagcag gtatagaatg gctctgaccc ctacatcagt acgaacgtta
      301 cagcactacc ttgaagaggc aatctttgtt ggacaggcac acaagttagg tgtattccgc
      361 cagcttggca ataaacccga tactgcatct gcacttgccc ggcgaatgca gtttgacaca
      421 cgggcaacat ggatattatt agaggcactg gttgagatgg gctatttgca aaaggataac
      481 gatatctata gccccacaca atactgcatt gatcatctgg taaacgaaga aagcgacgat
      541 tacgatggcg atttcctgaa atttttgtta tacctgatgc atccatggca gacacttcct
      601 tacgtgctga agaccggtac tcctgatgaa tcaagcttca aggagctatc catgcaggat
      661 tttataaaag ggatggattc gccatggaag aaaaaaataa cgccggaggt agttagcctg
      721 tgcctttctt attgcactgc ttcatcatgc attgacatag gcggagcccc gggaactatc
      781 gccagagaat ttgtaaaacg aggagttaaa accatcatat ttgatcttcc gcaatcgctg
      841 gcaataacac aggatgcatt aaaggatata ccagatatag aaataatagc cggtgatgcc
      901 accgttgctc tgcctcactg taaggttgat atagcatttt taggcaatct gtgtcatggg
      961 caatcgccgc aggataacca gaagattatc agcatgtgct atgacattgt aaatcctgat
     1021 gggatcattg caatctttga caatctcagg gatgaaagct atttaggagc aacgctggca
     1081 ctccatatgc ttacccagag caaacatggc aatatatatg cacgcgaaga atactttgca
     1141 tggttacata atgcaggatt taaggaacca caggtattac agctatccga caaagcatgg
     1201 cagatgatta tagcaaaaaa ataacattat ttatgaggtg gcagcggcaa aagcattgag
     1261 actttcttag aatattcgcg aaattcctgg ccaaaatcct gttctaaacg tttttcttca
     1321 gtttgcttta aatatatgat gatgactgga acaaacgcca gaatgaaaac aacaacaact
     1381 aacgaattga gatacaacgc aaatgcaata taacaacata atgcaccaaa aaccatagga
     1441 ttacgcgtat aacgatagag gccctttacc accagatgct ttgtacgcgg actaatggaa
     1501 acattaaacg catccacagg accgcctttg ccaattacaa gcaaagcaac atttgaccat
     1561 atcataaaga taagcccaat aagaaacagc accaccgaca ccagcacccg tataatagta
     1621 ttgccaatga tgcctgcagg tatcagttga tgaaaatagc tggcaatgaa atagatcact
     1681 gcgggcaaac ataccaaaaa aatagtaaat ccaaccgcat aaccaagaat atggcggata
     1741 actgttctaa gctccatgct attcaccttc taaaattttt ttattgtact g
//
LOCUS       DAOOJH010000221         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_53720_length_1773_cov_3.944703, whole genome shotgun sequence.
ACCESSION   DAOOJH010000221 DAOOJH010000000
VERSION     DAOOJH010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53720_length_1773_cov_3.944703"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(441..>1773)
                     /locus_tag="PLJ75_13300"
     CDS             complement(441..>1773)
                     /locus_tag="PLJ75_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPD06232.1"
                     /translation="KTIVDALTAEIVPAVLATRFYCKNAQRFLNDRKLPMGSILFVNK
                     RSKIVRVPYGVVGIISPWNYPFSIPYSEIIMALLAGNAVILKTASETQCVGHILKECI
                     ETAELPPHIFTYINMPGSIAGDAFIEAGIDKLFFTGSVNVGKYLMNKASERLIPLVLE
                     LGGNDPMIVCDDADLYRAAAGAVWAGMQNAGQSCGGVERIYVDRKVYNEFLTILKGMV
                     ESLRVGDGSDISSDIGAMTTRRQMEVVQRHVDDAVKKGATIYAKSKVNSKKGQFMPCM
                     VLADVNHDMLTMKDETFGPIVGVMPYDTIDEAIALSNDSYLGLTASVWSKNRSKAMKI
                     GRQIKAGAVTINDHLMSHGLAETPWGGFKESGIGRTHGDLGFAEMTQPQVIVNDILPF
                     ARRNFWWKPSDKRQYEGLKGAIDVFYAQGIFNKMKALYKLSKAFMTTFIRL"
BASE COUNT          559 a          409 c          250 g          555 t
ORIGIN      
        1 cccaataatt tctctaccag gaattctaac aacatcctta tacaaattat ataatgcgaa
       61 ttcaccatct tatagtcttc tgagaatttt tttgcacgat atatagttat aataagttgt
      121 atggaatata cctttaaaat taattttttt agactggtga catcatacca gaataactat
      181 cgcccaaaaa aataaaatac aattgaagca atgccttatt aaacattacc caacaactgc
      241 acttgtatcg ccagtatgat aacctgttat ttgttttaca atattataca acatagctat
      301 agatcacctt tccaccaata aacataatgg tgattctatg cttaatgctt aatgcaagta
      361 tttagctaca ccactaacta ttcacaaaac atcacgcata ctggaaatgc atggtaatgt
      421 gtcaaaatat aagtaactaa ctatagcctg ataaacgttg tcataaaagc ttttgacaac
      481 ttatacaatg ccttcatttt attaaatatt ccttgagcgt aaaaaacatc aatagctccc
      541 ttcagccctt catattgcct tttatcagaa ggcttccacc agaaatttcg gcgtgcaaat
      601 ggtaaaatat catttacaat gacctgcggc tgtgtcattt ctgcaaaacc aagatcgcca
      661 tgagttcttc ctatcccact ttctttaaat ccaccccatg gggtctcggc caatccatga
      721 ctcattaaat gatcatttat tgttaccgca cctgctttta tttgcctgcc tatcttcatg
      781 gctttagatc tgttctttga ccatactgaa gctgtcaatc caaggtacga atcatttgac
      841 aaagctattg cctcatcaat tgtatcatac ggcataacgc ctacgatggg accaaaggtt
      901 tcatccttca ttgtcagcat atcatgatta acatccgcaa gcaccataca gggcataaac
      961 tgcccttttt tactattaac ttttgatttt gcatatatcg ttgcaccttt tttaacagca
     1021 tcgtcaacat gacgctgtac cacctccatc tgccgccttg ttgtcatcgc accaatatcg
     1081 gatgagatgt cactcccatc accaacacgc aacgattcta ccataccttt taagattgta
     1141 aggaattcat tatatacttt tctatcgaca tatattcttt caacccctcc gcatgactgc
     1201 cctgcattct gcatacctgc ccacacggca cctgcagcag ctctatacag atctgcatcg
     1261 tcacacacaa tcattgggtc atttccacca agttcaagta ccagaggaat taatcgttca
     1321 gatgctttat tcataaggta ttttcctaca tttaccgaac cagtaaaaaa tagcttatct
     1381 ataccagcct caatgaatgc atcaccagca atactacccg gcatattgat ataggtaaat
     1441 atatgagggg gtaattcggc tgtttcaata cactctttca aaatatgccc cacacattgt
     1501 gtttctgaag ctgtctttag tattactgca ttaccagcta acaatgccat gataatttca
     1561 gagtaaggaa tagaaaaagg ataattccat ggagatataa tacctacaac accataggga
     1621 acacgtacta ttttacttcg cttattgaca aacaatatag aacccatggg tagttttcta
     1681 tcgttcaaga atctctgtgc atttttacaa taaaaccgtg tagcaagcac agcaggaact
     1741 atctcagcag tcaatgcatc aactattgtt tta
//
LOCUS       DAOOJH010000222         1743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_55108_length_1743_cov_6.895142, whole genome shotgun sequence.
ACCESSION   DAOOJH010000222 DAOOJH010000000
VERSION     DAOOJH010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55108_length_1743_cov_6.895142"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..889
                     /locus_tag="PLJ75_13305"
     CDS             101..889
                     /locus_tag="PLJ75_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPD06233.1"
                     /translation="MKKYFFINAITFTAIMLMCFIHAHSQNMPSAFSTVPIKNFTPKI
                     WDEDFSLYMIGSEQEILLITTPYQTILSTKNNSNRHAVLIKAGKNKDYAELPLNKDMK
                     DTRLLQLTHPLVQSYAKKIKASSNIVNEVEQLIYTTIETKTEGIPIIPAPMILETKKG
                     DCTEHAVAAIALLRALKIPARAVVGMIAAREYAGRQNVFVFHMWAEAYYNNTWILVDA
                     TRPGTKKLNLYIALSYHSLETEMPLEFLEAMAAIQNLTVTYLSE"
     gene            1071..>1743
                     /locus_tag="PLJ75_13310"
     CDS             1071..>1743
                     /locus_tag="PLJ75_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06234.1"
                     /translation="MRLFHLTVTITLIIIAFAINTSLYPQGAKNYFDAGCRYYVHKNW
                     EKAKENFLKDIEVTDRGDSYYFVGEIEKIQGDLDSALHYFENAVTRKMTPKYRNLAYW
                     NLIVIYESRSDYAKTVRTCKYFYEVYGEASAKTKAENLINKLMWTENEDAKKEYEAGL
                     ELKLKDYTTALQHFRQAVYLDNSFLSPVFEIGMYYYSQENYREALSYFNQILYKIPFY
                     GELQML"
BASE COUNT          599 a          309 c          308 g          527 t
ORIGIN      
        1 aaataaaaaa tattataatc tatttagatg ataaaggagt ggtactgaaa acctctatta
       61 ggatgcttaa tatgaaactt gatgttatac gcaagggatt atgaaaaaat atttttttat
      121 caatgcaata acctttactg ctatcatgct gatgtgcttt attcatgcac attcgcagaa
      181 catgccatca gctttttcaa cggtgccaat aaaaaatttt acccctaaaa tctgggatga
      241 agatttttct ctttacatga ttggatctga gcaagaaatt ttgcttataa caacgcccta
      301 tcagactata ttgtctacaa aaaataatag caatcgtcac gctgtattaa tcaaagcagg
      361 caaaaataaa gactatgctg aactgccatt aaataaagac atgaaagata ctcgactttt
      421 gcagctaact catccattgg tacagagcta tgcaaaaaaa attaaagctt ccagtaatat
      481 tgtcaatgag gttgaacaac tcatctatac tactatcgaa acaaaaacag aaggtatacc
      541 aataattccc gcaccgatga tactggaaac aaaaaaaggc gattgtacag aacatgccgt
      601 tgctgccatc gctttactca gggctttaaa gattcctgcg cgtgcagtag tggggatgat
      661 agctgccagg gaatatgctg gtagacaaaa tgtatttgta tttcacatgt gggcagaagc
      721 gtattataac aatacatgga ttctggttga tgcaacccgc cccgggacta aaaagctcaa
      781 tctgtatatt gcactatcct accatagcct tgaaactgaa atgccgctgg aatttcttga
      841 agctatggca gctattcaaa atcttacagt aacctatctt tcagaataat gcagtattat
      901 ctatgttcat tacaatacat tgtagtgttt gtgttatggt aattaccatt gctatgattg
      961 ctgttaaacc tgaggaagca ttccaattaa tgtattgaca tatttgtaat acaaaactgt
     1021 aatttataaa actatgccgc aaacatgcta atatcatcag ggattgtact atgagattgt
     1081 tccatttgac agtaactatc acccttatca taattgcctt tgctataaat acctctctct
     1141 atccccaggg tgctaaaaat tattttgatg ccggttgccg ttactacgtc cacaaaaact
     1201 gggaaaaagc aaaagaaaat tttcttaaag atattgaagt taccgataga ggtgattcgt
     1261 actactttgt tggcgaaatt gaaaaaattc agggtgattt agattctgca ctgcactatt
     1321 ttgagaatgc tgtcacccgc aaaatgacac ccaaatatcg taatctggca tactggaact
     1381 taatcgttat ctatgaatca aggtctgatt atgcaaaaac tgtcaggacc tgcaaatatt
     1441 tttatgaagt gtatggcgag gcttcggcaa aaacaaaagc tgaaaatctt atcaataaac
     1501 tgatgtggac cgaaaatgaa gacgcaaaga aagaatatga agctggattg gaattaaaat
     1561 taaaagatta tactacagcg ttacaacact tcaggcaggc tgtctacctt gataattcat
     1621 ttctttcacc tgtttttgaa atagggatgt attattattc acaggaaaac tatcgtgagg
     1681 cattatcgta ttttaatcaa atactgtaca aaattccatt ttatggtgaa ctccagatgc
     1741 tgg
//
LOCUS       DAOOJH010000223         1707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_56893_length_1707_cov_4.236077, whole genome shotgun sequence.
ACCESSION   DAOOJH010000223 DAOOJH010000000
VERSION     DAOOJH010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56893_length_1707_cov_4.236077"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLJ75_13315"
     CDS             complement(<1..1084)
                     /locus_tag="PLJ75_13315"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HPD06235.1"
                     /translation="MQNKKLFLSMLITSGIGIIISAYLLYIHYFPLIPDAVFYSLCTA
                     GSTFDCNAVNTSAYAMLFGMPLAGWGVAFYLFFIAVILIYTVKTKHVIIEFGMLWLAM
                     FAVLLSVVLGYISFVKIQKFCSFCMVLWLCNVALLMLVLMYIASLYKGLINGINTIHD
                     FTVTTLFGNNKLRGTIVVLVIAGVVSLLVAWGIDETLQLTYNQKEKQREAKILQEFKK
                     EYKMFERVAIPVDSTIPVIGKKEYPVHITVFFDFNCGACHRAIAVLSELAKKYEGKVA
                     VYLRHFPLDGTCNRFIQHKKDGASCIASGIAYALYGGSSYHDYIMQLMSYRGKVDAIA
                     IGKVVSDLQKDYADLKKLSNTDTIKKL"
     gene            complement(1271..>1707)
                     /locus_tag="PLJ75_13320"
     CDS             complement(1271..>1707)
                     /locus_tag="PLJ75_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06236.1"
                     /translation="GHISKNGKSSLVIARVKPQYILQRDTYSFLQGDSSWKNTVTAAI
                     QLYGDVAGECSLGYDEDYKFFYIVYCQLFSGNIVMSIAPSIEQLPFAKKIIVYTPAKL
                     KGTSMIYYSAKEILHQKNQWYIIYINPIEYQPYLMQVKINIH"
BASE COUNT          557 a          351 c          270 g          529 t
ORIGIN      
        1 aaagcttttt tattgtatca gtgttcgata gtttttttaa atcagcataa tctttttgta
       61 aatctgaaac caccttgcct atagctatcg catccacttt tccccggtat gacattaact
      121 gcatgatata atcatggtat gaactgcctc catacaatgc atatgcaata cctgatgcta
      181 tgcatgaagc accatccttt ttatgctgaa taaacctgtt acatgtacca tccagaggaa
      241 aatgccgtaa gtaaactgca accttccctt catatttttt tgccagttca gaaagtacag
      301 caattgctct atgacaggca ccgcagttaa aatcaaaaaa aacggtaata tgaacaggat
      361 attctttttt accaataacc gggatagtgc tatcaaccgg tatggcaacc ctttcaaaca
      421 ttttgtattc ttttttaaac tcctgtagta tttttgcttc acgctgtttt tctttctggt
      481 tatatgtaag ctgtaatgtt tcatcaatac cccatgcaac cagaagtgac acaacccctg
      541 ctatgacaag tacaacgatt gtcccacgta gcttgttgtt gccaaacagg gttgtaacag
      601 taaaatcatg gattgtattg atgccattga ttaacccttt atacaacgat gctatataca
      661 tgagcaccag cattaacaat gcaacattgc atagccacag taccatgcaa aacgaacaaa
      721 atttttgtat ttttacaaat gatatataac ccaatacaac cgataacaac acagcaaaca
      781 tagctaacca caacatacca aattcaatga tgacatgttt tgtttttact gtgtaaatta
      841 gtatcacagc aatgaaaaat aaataaaatg ccacacccca tccagccagc ggcattccaa
      901 aaagcatggc atacgcgctg gtattaaccg cattgcaatc aaaggtacta cccgcagtac
      961 ataaagaata aaacacagca tcaggtatga gcggaaaata atggatatac agcaaataag
     1021 cagagataat gatccctata ccggaagtaa taagcattga taaaaataat tttttgtttt
     1081 gcatgaataa cttctccaat aataggttta tgtgccaatg tcatgctgga ctcgtttcag
     1141 tatctattcc ttcattttag atcctgcatc agaattcagg atgactgtcg ccaactattt
     1201 tcatcccatg gttggttagc ttgccaaact atgactcatg cggcgcaaac acaagaagct
     1261 ttcttaagat ttaatgtata ttaattttca cctgcataag atacggttga tactcaatcg
     1321 ggtttatata gattatatac cattgatttt tctgatgcaa tatttctttt gccgaataat
     1381 atatcataga ggtacctttt aatttagccg gcgtataaac aattattttt tttgcaaatg
     1441 gcaattgctc tatcgaggga gcaatgctca taacgatatt gccactgaaa agctggcaat
     1501 acacaatgta aaaaaattta tagtcttcat cataccccag tgagcattcg cctgcaacat
     1561 cgccgtaaag ttgaattgca gcagttacag tatttttcca ggaactatcg ccctgtaaaa
     1621 atgaatacgt atccctttgc aaaatgtatt gtggtttaac ccgtgcaata acaagtgaac
     1681 ttttaccgtt tttgcttata tgcccaa
//
LOCUS       DAOOJH010000224         1692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_57662_length_1692_cov_5.031155, whole genome shotgun sequence.
ACCESSION   DAOOJH010000224 DAOOJH010000000
VERSION     DAOOJH010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57662_length_1692_cov_5.031155"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLJ75_13325"
     CDS             <1..247
                     /locus_tag="PLJ75_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06237.1"
                     /translation="IVFSPGAYSNELLKLTSGTPLAVYALTLKMENILTRGVFKGFQR
                     HAGILTGMLDINYVYNSMPPVHGQIYPKIPLTPVKEF"
     gene            419..1669
                     /locus_tag="PLJ75_13330"
     CDS             419..1669
                     /locus_tag="PLJ75_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06238.1"
                     /translation="MKLNKISVLLVLIFSIITFFNCSGDNGTSIIIINVGLQNQQAHN
                     SFFYKLINFIQKPVYADPPSNIQSITINITAADFSPITRTYTPPYGIFRIEAPSGIQR
                     AIEVLASTPSATIRGFVIVDLKPGEQTVDIAMHLYSTKILIPDYNSHRLVQIDDMTGS
                     GFRDINGGTISFPGGTIYPYDVDFDDIGRIYIANYGFSTEQNVVIRLDDIEDTTYTSL
                     GTGGLNGIRSVAVDRENKLVYYATSSQLYRCDLNGSNLKSDYTMTDISFIKSIAYHNS
                     FLYIACTYDSNYRIVKYDTTANGQIAGYSLASLHSAWDVIIKNDLCIVADDDESTSND
                     SIAILDPDDLSLFNKYGTGTSLPSPLPGQFWGPRRFVAILNDRFYLIDERDGMMDNLV
                     SFSDLTFSNWERYDDTIFMFFSFC"
BASE COUNT          527 a          291 c          325 g          549 t
ORIGIN      
        1 gatagtgttt tcccccggtg cttacagcaa tgagttgctc aagcttacca gtggcacacc
       61 gcttgccgtg tatgcgctta cccttaaaat ggaaaatatc ctgacacgag gagtgttcaa
      121 gggctttcaa cgtcatgcgg ggatattaac cgggatgctt gacataaact atgtatacaa
      181 ctccatgccg ccagtgcacg ggcagattta ccctaaaata ccgcttacac cggtaaagga
      241 attttgatgc ggtaaaaaaa tagtaaaagg ctgtagcatt gaaagttgcc aatattatta
      301 atcgttatta ttaataatag ttgacattgt actatatata ctttacatta aagttgtata
      361 taattgctaa actcttcctg gctcataaat attaatattt gagattacga ggtttccaat
      421 gaaattaaac aaaatttctg tgttattggt attaatattc tcaatcataa ctttttttaa
      481 ttgcagcgga gacaacggta cttcaatcat aataattaat gtaggcttgc agaaccagca
      541 ggcacataat tcattttttt ataagctaat caactttatt caaaaaccag tatatgcaga
      601 tccaccatca aatatacaga gtataacgat taatattact gctgccgact tttctcccat
      661 cacaaggaca tatacaccac cgtatgggat ctttagaatt gaggcccctt caggaatcca
      721 gcgagccatt gaagttttag ccagcactcc ttccgctact attcgcggat ttgtaatcgt
      781 tgatttaaaa ccgggcgaac aaacagtaga cattgccatg catttataca gcacaaagat
      841 actgatacct gattataata gtcataggct tgtgcaaatt gatgatatga cggggagcgg
      901 atttcgtgat ataaatggag gtactattag ttttccagga ggtactatat atccctatga
      961 tgtggatttc gatgatattg gcaggatata tatcgcaaat tatggttttt ctacagagca
     1021 gaatgttgtg atacgtcttg atgacataga agatactact tatacttcat tgggaactgg
     1081 tggtttgaac ggtattagat cagtagcagt tgatagggaa aataaactgg tgtattatgc
     1141 aacaagcagt cagctttatc gctgcgatct taatggcagc aatttaaaaa gtgattatac
     1201 catgacagat atatcattta taaagagcat tgcgtatcat aatagctttc tatatatagc
     1261 atgtacctat gattcaaatt atcgaattgt taaatatgat acaacagcaa atggacaaat
     1321 tgcgggttat tctttggcat ctttgcattc agcatgggat gtcatcatta aaaatgacct
     1381 atgtattgta gcagatgatg atgaaagtac ttccaatgat tcgatagcta ttttggatcc
     1441 agatgattta tcattattca ataaatatgg aacaggtaca agcctcccct cgcctttgcc
     1501 tggacaattc tggggtcctc gcaggtttgt tgcaatatta aatgatagat tctatttgat
     1561 tgatgaaagg gatgggatga tggataatct tgtctcgttt tccgatttaa ctttcagtaa
     1621 ctgggaacga tatgatgata ccatctttat gtttttttcg ttctgttaaa cagtgaatct
     1681 tgttttttat ac
//
LOCUS       DAOOJH010000225         1680 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_58303_length_1680_cov_5.817231, whole genome shotgun sequence.
ACCESSION   DAOOJH010000225 DAOOJH010000000
VERSION     DAOOJH010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58303_length_1680_cov_5.817231"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1193)
                     /locus_tag="PLJ75_13335"
                     /pseudo
     CDS             complement(1..1193)
                     /locus_tag="PLJ75_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
     gene            complement(1277..>1680)
                     /locus_tag="PLJ75_13340"
     CDS             complement(1277..>1680)
                     /locus_tag="PLJ75_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006555248.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPD06239.1"
                     /translation="DGSHIRTLLLTFFFRYMPEIIANGYLYIAQPPLYNIKAGKEQYY
                     AYSDEERDSLVKKLDKSKVAIQRYKGLGEMNPEQLWETTMDPEKRTLLQVTLEDEVEA
                     DEVFSVLMGDAVEPRRKFIEENAHMVENLDL"
BASE COUNT          472 a          367 c          269 g          572 t
ORIGIN      
        1 gcttcttcaa ccgtttttga tcttctaata atcgcaatca cttcatcaat attgtctaaa
       61 gctattttta aaccttctaa tatatgtgcc cgctcccttg cctttgccag atcatactgg
      121 gtgcgtcgtg ttacaacaat ctttcggtga ttgatataat ggaatatcaa tccttttaaa
      181 tccagcagtt ttggctgatt attaacaaga gccaggaggt taatcccaaa ggatgtctgt
      241 aactgtgtat gtttatagag ttgattgatg ataatgtttg ttactgcatc cttgcgacat
      301 cctatgacaa tgcgtaatcc tttccgatcc gactcatccc gcagttcatt gataccttca
      361 attaccttat tatttaccaa ttcagctatt tttgcaataa gagctgcttt gttcacctga
      421 tagggtaatt ctgttacaac aatcagtgac ttattttttt gttcttcaat atgcagtttg
      481 gctctaatta ttacacttcc ctttccttta gcatacgctt tcaccatttc ttctgaacca
      541 acaatggtag ctccggtagg gaaatcaggt cccttaacaa atttcataag ttgcttaact
      601 gttgcatcag gcgtatcaat cataaacaca attgcatcta ttatttcgcc taaattgtgt
      661 ggaggaatat tggttgccat accaacggca atacctgaag aaccattaac cagcaaattg
      721 ggaaaagcag aaggaagtac tacaggctct ttgcgggttt catcaaaatt tgatacaaaa
      781 tcaacagtat ctttatcaat atctttcagc aactcctgag caatcggtga aagacgagcc
      841 tctgtataac gatatgctgc cggaggatct ccatctacag aaccaaaatt accctgacca
      901 tcaatgagtg gtaatctcat cgaaaaatcc tgtgccattc taacaagcgt atcatacact
      961 gcggcatcac catggggatg ataattacca ataacttccc ctactatctt tgctgatttt
     1021 acatatggtc tgtcactgcg ccatgcacgc tcattcattg catgtaagat acgccggtgt
     1081 acaggtttca ggccatcacg aacatccggc agtgctcgtc caacaatgac gctcatggca
     1141 taatcaagat atgagcgttt cattgattct tcaatctcta tatttaccac ttttttgtct
     1201 ttttttacat cttccttcat gttaactatt tacctattat ttgaaaagat ttctgcataa
     1261 tgctcgatag ttatggttat aaatctaaat tctctaccat gtgtgcattt tcttctatga
     1321 attttcgtct tggttcaact gcatcaccca ttaaaacaga aaaaacctca tctgcttcta
     1381 cctcatcctc cagagtaacc tgcagcaatg tccttttctc agggtccatg gttgtttccc
     1441 ataattgttc gggattcatt tcacctagcc ctttgtatcg ttgtattgca acttttgatt
     1501 tatctaattt ttttactaag gaatctcttt cttcatcact gtaagcatag tattgttctt
     1561 tgccagcctt aatattatac aatggaggct gagcaatata taagtatcca ttagcaataa
     1621 tctcaggcat atatctaaaa aagaaagtga gcaataacgt acgtatatgt gatccatcaa
//
LOCUS       DAOOJH010000226         1547 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_65982_length_1547_cov_7.238606, whole genome shotgun sequence.
ACCESSION   DAOOJH010000226 DAOOJH010000000
VERSION     DAOOJH010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65982_length_1547_cov_7.238606"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(550..1518)
                     /locus_tag="PLJ75_13345"
     CDS             complement(550..1518)
                     /locus_tag="PLJ75_13345"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HPD06240.1"
                     /translation="MNVIVSFSPLPPEFIVNILNSHGITDIEVINVYDKPKDDILLNV
                     SRADIIIGDYTFKHSIDREMIAAMKKVKLIQQPSAGYQHIDIMACKEAQISVANTAGA
                     NAISVAEYTILAALCLLKNIFFASRTTAKGEWQQMQIRPVELYGKTWGIVGMGRIGKL
                     LAQRLTVFGVTILYYDPWRLSSEAEQLMSVTYVPFYELIKTSDIISLHCPLTEETKGL
                     LGKEELSAMKSSAIIINVARGEIIDENALADALKEGRIAGAALDVFSDEPINSNNPLL
                     KVNSDRLLLTPHVAGVSIESQMRIINLTIENIIAVISGREPVNIIV"
BASE COUNT          459 a          310 c          263 g          515 t
ORIGIN      
        1 ctactgcttg atgacattga tgcatggata ctgaaagcaa aaaatgagtg gatgattaag
       61 caccgcttta atgcccacga agtaatgatt gactgtacta tggtcattgt atcgtaaaat
      121 tctactgctg ttttaaatga tcgatgcaat ctttttttgt atttcctcaa ccattacttt
      181 gtactgttca atgctcaata tatgaggtcc catagtatca atatcaggca ttttcttcat
      241 aagagctatc gccatgtcat atatatatgt tctttttatt attgtttaca taaccgtatc
      301 cataaacccc tttaaccata aaagctttag ccagagcggt gtggggtaaa atgctaatgt
      361 gttggttatg ccaatcgaac ttatggtgaa acttacccgg gttatatcca tggccaaaga
      421 aaacgtagca gaagaaccaa ccgtccactg taagagccat ccgctgaggt atggttgcat
      481 tgaattgttg tgccttcacg atttttacct tgattaaacc tgatttacct atagttatga
      541 agcacttttc tacactatta tattaacggg ttctctgcct gatataaccg caataatatt
      601 ctcaatcgtt agattaataa ttctcatttg tgattcgata ctaacccccg cgacatgagg
      661 agtaagaagt aacctgtcgc tatttacttt taacaaaggg ttatttgaat tgattggttc
      721 atcgctgaaa acatcaagcg cagcacctgc tattctccct tctttcaatg catcggcaag
      781 tgcattctcg tcaataatct cgccacgagc tacattgata attattgcag atgatttcat
      841 tgcggataat tcttctttgc ccagaagtcc tttagtttct tctgtaagag gacaatgcaa
      901 gctaataata tcagaagtct ttattaattc atagaaagga acataggtaa cgctcatcaa
      961 ttgctctgct tctgatgaca acctccatgg atcataatat agaatagtaa caccaaacac
     1021 agtaaggcgt tgggctaata attttccaat acgtcccatg ccaacaatac cccatgtttt
     1081 cccataaagt tcaacaggtc gtatttgcat ttgctgccat tcgccctttg ctgttgtacg
     1141 tgatgcaaag aaaatatttt ttaacaaaca caatgcagca aggattgtat attctgctac
     1201 cgaaatagcg tttgctccag cagtatttgc aacactgatt tgcgcttctt tacaagccat
     1261 tatgtcaata tgctgatacc cggcacttgg ctgttgtatt agcttaactt ttttcattgc
     1321 tgctatcatt tcacggtcaa tggagtgttt aaatgtataa tcaccaatga taatatcagc
     1381 acgactgaca ttcaatagga tatcatcttt aggtttatca tagacattaa taacttcaat
     1441 atctgtaatg ccgtggctat tgagaatgtt aacaataaat tctgggggca aaggtgaaaa
     1501 cgatactatt acattcactt tatctccctc cttaacattt tatttat
//
LOCUS       DAOOJH010000227         1500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_69131_length_1500_cov_10.248443, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000227 DAOOJH010000000
VERSION     DAOOJH010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69131_length_1500_cov_10.248443"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PLJ75_13350"
     CDS             <1..855
                     /locus_tag="PLJ75_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HPD06241.1"
                     /translation="KFSEASRDLTAIIEETSSAYEEMSSSFEMIVNTIKSQVEQATAV
                     KENIDSINQAGNNVSQQINALANTFNEAITGIEKGRETMQHAVAAITDISNYLQTVES
                     TVSAINEVADQINLLALNAAIEAARAGEHGRGFAVVADEVNKLADQTAALVKSIQSTI
                     DQYTGKVKNEIQFISHTANLFETVRDNILNTGKVLTTTQEFTKNLNDQNANIQEKIQK
                     LVQLSNEIYTTSNEQQITIDELTKAINAINEVASHTSDESVNIQDISEKLEENAAKLL
                     KNIEFFKL"
     gene            complement(914..>1500)
                     /locus_tag="PLJ75_13355"
     CDS             complement(914..>1500)
                     /locus_tag="PLJ75_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPD06242.1"
                     /translation="HGNINLKSGIWIAIGSIIGAQVGTGFSAKIPEISLGAAFGIFMA
                     GMGIAMWKKGINRESVASTTKKILNLETQTQKIMAVLILGFFVGIMTGIFGAGGGVMI
                     LLILIFVLNFPIHMAIGTSSLLMTITACSGAFGYALHGNIRILPGLITGISAAGSGVA
                     GALFANRANEQILAMVIGILYIMLGIVMTILSLW"
BASE COUNT          543 a          300 c          263 g          394 t
ORIGIN      
        1 aaattttcag aagcttcgcg ggatcttact gcaatcattg aagaaacaag ttctgcttat
       61 gaagagatgt cttcctcatt tgaaatgatt gttaacacga taaaatcgca ggtagaacag
      121 gcaactgctg taaaagaaaa tattgattca atcaaccagg caggcaataa tgtatcacaa
      181 cagattaatg cactggcaaa tacatttaac gaagctataa ctggcataga aaaaggaagg
      241 gaaactatgc aacatgctgt tgcggcaata accgatatat caaattacct gcagactgta
      301 gagtcaacgg tttccgccat taacgaagta gccgatcaaa ttaacctatt agcactcaat
      361 gcagcaattg aagcagcccg cgcaggtgaa catggcagag ggtttgctgt tgttgcagat
      421 gaagttaata aattagctga tcagactgct gcacttgtca aaagcataca atccaccatt
      481 gatcaatata ccggtaaggt aaaaaatgag atacaattta ttagccatac agccaatttg
      541 tttgaaacag tacgtgataa cattctaaac accgggaagg tattgacaac tacacaggaa
      601 tttacaaaaa atcttaatga tcaaaatgca aacattcagg agaaaataca aaagcttgta
      661 caactttcaa atgaaatcta cacaacatca aatgaacagc aaattacaat agatgagcta
      721 acgaaagcaa ttaatgcaat caatgaagtt gcatcacaca cttccgatga atctgtaaac
      781 atccaggata tatctgaaaa attagaagaa aatgctgcta agctattaaa aaatatagaa
      841 ttttttaaac tttgatgagg aatgagatag acacatacta tcgcccaaat aatggtagcc
      901 ttccagagta caattaccat aacgataaaa tggtcattac tataccaagc ataatgtata
      961 gtattccaat caccatagcc agtatctgct cgtttgccct attagcaaaa agagcacctg
     1021 ctacaccact gcctgcagca ctaatacctg ttattaatcc aggaagaatt cttatattac
     1081 catggagggc gtatccaaat gcacctgaac aggcagttat tgtcatgagc agtgatgaag
     1141 taccaattgc catatggata gggaaattga gaacaaaaat caggatgagc agtatcataa
     1201 ctccaccacc agcgccaaaa atgccagtca tgatacccac aaaaaagccc agtatcaaaa
     1261 cagccatgat tttttgggtt tgagtttcaa ggtttagtat tttctttgtt gtgcttgcaa
     1321 ccgattcgcg atttatccct tttttccaca ttgcaatacc catacctgcc ataaaaattc
     1381 caaacgctgc gccaaggctt atttcaggaa ttttggccga aaagccagta ccaacctgag
     1441 ctcctatgat tgaacctatg gctatccata ttcctgattt taaatttata ttaccatgtt
//
LOCUS       DAOOJH010000228         1389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_77739_length_1389_cov_10.223388, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000228 DAOOJH010000000
VERSION     DAOOJH010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77739_length_1389_cov_10.223388"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..826
                     /gene="lexA"
                     /locus_tag="PLJ75_13360"
     CDS             221..826
                     /gene="lexA"
                     /locus_tag="PLJ75_13360"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPD06243.1"
                     /translation="MVKPLTKKQEAIFLFIKQSIKKTGFPPTVREIGNQFSITVKGAY
                     DHLKAIEKKGYLRCEAGKSRAIELLVNKDDVYPDVCSIPVVGTIAAGIPVLAQENIES
                     YIHLPSGLLPQGTLFALRIKGDSMTGAGIFDGDIAVIKQQSDANNGAIVAALLDDEAT
                     LKILKKTERKVHLLSANEKYGVITADTISILGTLAAIFRTY"
     gene            911..>1389
                     /locus_tag="PLJ75_13365"
     CDS             911..>1389
                     /locus_tag="PLJ75_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06244.1"
                     /translation="MHRVYDKQFHYGHLIDQFYKIETKFDSLLFFYNNREKIVSLFNY
                     DVVIIVELSHLFDQIAYQIQECSSYYDKYIIDILYRKAESYLAFMEGLDYYNKFLLIH
                     NLIYDDIVMIWRHSLSNFRERCINDFHQQKSLQYTITTALLSMHDETLIPFFYDIALL
                     "
BASE COUNT          466 a          204 c          249 g          470 t
ORIGIN      
        1 ggagtttaat attttttgta ataaaattgt tgttgaatta attgaaagct atttagttaa
       61 atactcataa tatatatata tatatatata tattattttc atgggaagca attggtgact
      121 atttaaaaac gttatgaata tataacagta tgaatgaaaa atgtaattga caataactat
      181 gcattagtat agtatagcct taaatcagga aggtaagagt atggtgaagc cattaacaaa
      241 aaaacaggaa gccatttttt tattcataaa acaatcaatt aaaaaaacag gattcccgcc
      301 aacggttcga gagataggca accagttttc tattactgtg aaaggtgcct atgatcattt
      361 aaaagcaata gaaaaaaagg gatacctgcg ttgtgaggct ggtaaatcgc gtgctattga
      421 acttttagtt aataaagacg atgtgtatcc tgatgtttgc tctataccag tagttggtac
      481 catagctgcc gggattccag tattagcgca agaaaatatt gagtcatata ttcatcttcc
      541 atctggttta ctaccacagg gcacgttatt tgctttaagg attaaaggtg actctatgac
      601 tggtgctggt atctttgatg gggatattgc tgtcattaaa caacaatcag acgctaacaa
      661 tggtgctatt gttgcggctc tccttgatga tgaagctacg ctcaagatac ttaaaaaaac
      721 tgagcggaag gtacatcttt tgtctgcaaa tgaaaaatat ggtgttataa ctgctgatac
      781 tatctcaata ctgggcacat tagctgctat attcagaaca tattaatatt aaaaaatagt
      841 ggacatgatt ttcaactatc tcattatggt ttaaatcgtt aaggatacat ggatggggta
      901 gttaccgccg atgcatcgtg tttatgataa acaatttcac tatggacact tgatagacca
      961 attttataaa atcgaaacta agtttgatag cttgcttttt ttttataata acagagaaaa
     1021 aatagtttca ttgtttaatt atgatgttgt tatcatcgta gaattatcac atctttttga
     1081 tcagattgcc taccagatac aggaatgttc cagctattat gataagtata tcattgatat
     1141 cctttaccga aaagctgaga gttatctggc ttttatggaa ggattggatt attataataa
     1201 atttttgctt attcataatt taatatatga tgatattgtc atgatatggc gacattctct
     1261 cagcaatttt cgtgaaaggt gcattaatga ctttcaccag cagaaatcat tgcaatacac
     1321 tataaccact gcactgttat cgatgcatga tgaaacactc ataccctttt tctatgatat
     1381 agcactgct
//
LOCUS       DAOOJH010000229         1356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_80776_length_1356_cov_8.318217, whole genome shotgun sequence.
ACCESSION   DAOOJH010000229 DAOOJH010000000
VERSION     DAOOJH010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80776_length_1356_cov_8.318217"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLJ75_13370"
     CDS             complement(<1..295)
                     /locus_tag="PLJ75_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014165832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPD06245.1"
                     /translation="MQSKNKKLMTIHGYSILNYYSIQKGLSMDHHHDHSHEHEYSQGS
                     FILVVVLNVIITAAEYIGGIISGSLALISDAGHNLSDVISLILGYIGQKVSQHK"
     gene            397..1005
                     /locus_tag="PLJ75_13375"
     CDS             397..1005
                     /locus_tag="PLJ75_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06246.1"
                     /translation="MKTAICAAIICSVMVFPAYSQNKNAKPQTAKEEKTTQPKQAKNI
                     AQGYGNVKWGSYLNDVKNNVAGRIVYMDEKRIIISRDGDLEYRYGFFYPEYNQTATEP
                     QQPKLFYVIIQFPYIALDDVKKKMEEKYGPATGESIKNNQGAYIWDSESTAVVLWVDS
                     YEKKPFCRKITYVSKIMAKDINEYYTTVFSKKEQEIIKKLQP"
     gene            complement(1007..>1356)
                     /locus_tag="PLJ75_13380"
     CDS             complement(1007..>1356)
                     /locus_tag="PLJ75_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06247.1"
                     /translation="VLVVSEQGNAILSRSRSAFNKGVIGVVAGNPVMVINNASSEKKL
                     YPVALTGSVLCKVDARQKGVKPGDLLVTSDTHGCAMPALIDSFDTIGTVFAKALTALD
                     DGIALIPVMIWKM"
BASE COUNT          452 a          267 c          252 g          385 t
ORIGIN      
        1 gtttgtgctg tgataccttc tgcccaatat aacccagtat aagcgagatg acatcggaaa
       61 ggttatgccc tgcatcagat ataagggcta agctgcctga aataatacca ccaatatatt
      121 cagccgctgt aatgatgaca ttgagcacta ccacaagaat aaaacttccc tgactatact
      181 catgttcatg tgaatggtcg tggtgatggt ccatagacaa tcctttttgt atgctatagt
      241 aattcaatat actatagcca tgtattgtca tcaatttttt attttttgat tgcatatatg
      301 tgccattttt ctgttaaaat actatggatt atcatgaaca tatttccgat agtattatgt
      361 aagagtgaga ttattctatt ttttaaggaa aatggtatga aaacggcaat atgtgctgca
      421 attatttgtt ctgttatggt ttttcctgca tattcacaaa ataaaaatgc aaagccccaa
      481 acagccaaag aggaaaaaac tactcaacca aagcaggcaa aaaatattgc acagggatac
      541 ggcaacgtta agtggggaag ctatttaaac gatgtaaaaa ataatgtagc gggtaggata
      601 gtttacatgg atgaaaaacg cattattata tcccgtgatg gtgatcttga ataccgctat
      661 ggattttttt atcccgaata caatcaaaca gctactgaac cacaacaacc aaaacttttc
      721 tatgttatta ttcaattccc ctatattgcc cttgatgatg taaagaaaaa gatggaagaa
      781 aaatacggac ctgcaaccgg tgaatccata aaaaataatc agggcgccta tatatgggac
      841 tctgaatcaa cggctgttgt tttatgggta gatagttatg aaaaaaaacc gttctgccgc
      901 aagataacct atgtaagcaa aattatggca aaagatatca atgagtatta caccacagtc
      961 ttcagcaaaa aggagcagga aataatcaag aagctgcagc cataatttac atcttccata
     1021 tcatcacagg aataagagct atcccatcat caagtgcagt gagcgctttt gcaaacaccg
     1081 ttccaattgt atcaaacgaa tcgataagtg ctggcattgc gcatccatgc gtgtctgaag
     1141 ttactaaaag gtcaccgggt tttaccccct tctggcgtgc atccaccttg catagcactg
     1201 aacctgttaa agcaacaggg tatagctttt tctctgagct ggcattattg atgaccataa
     1261 ccggattgcc agcaaccaca ccaatcacac ctttattaaa ggcacttcgt gagcgtgaaa
     1321 gaattgcgtt cccctgttca ctgaccacaa gaacat
//
LOCUS       DAOOJH010000230         1327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_83530_length_1327_cov_5.209906, whole genome shotgun sequence.
ACCESSION   DAOOJH010000230 DAOOJH010000000
VERSION     DAOOJH010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83530_length_1327_cov_5.209906"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLJ75_13385"
     CDS             complement(<1..241)
                     /locus_tag="PLJ75_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06248.1"
                     /translation="MPNYSLQEVIERSKGWFKHPYSRVAFIIFIAISLYLIISAIVVV
                     VLTKPDKEVKIPDVVGKRYSDVHNSLIRKGLKPHLK"
     gene            complement(379..585)
                     /locus_tag="PLJ75_13390"
     CDS             complement(379..585)
                     /locus_tag="PLJ75_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HPD06249.1"
                     /translation="MGRTLIVFGILFIIIGILLQLSPKFLGNLPGDVVFTRGRVTVYL
                     PFTTMIIISVILTIIINGIVRLLK"
     gene            937..>1327
                     /locus_tag="PLJ75_13395"
     CDS             937..>1327
                     /locus_tag="PLJ75_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPD06250.1"
                     /translation="MNNMDTIYNSDFSLKELIDNLPDLVLSVDYPSGVIRYANIRAID
                     RLGFSIKKVQKLKDLARVLLPRYRKKYLQFWRDIKGGILIPSITFEVKTQENIIIWVE
                     QKNIYIKHNDNVIRIHAVIRDITERKKL"
BASE COUNT          473 a          230 c          245 g          379 t
ORIGIN      
        1 attttaggtg gggctttaat cccttacgga taagactgtt gtgcacatct gaatagcgct
       61 tgccaacgac atcaggaatt ttaacttcct tatcaggttt ggtcaatact acaacaacta
      121 tcgccgaaat aataaggtac agtgaaatgg caataaagat gataaaggca acccggctat
      181 agggatgttt aaaccaccct tttgagcgtt caataacttc ctgcaacgaa taattaggca
      241 ttgtaacatt cctctgcgat gtaaaaaaga taattgttac tagtataaaa tggaaaattg
      301 aactcaagca aaatttacat aatgcgtcaa gaattatttt actatagcaa tgagtaaaaa
      361 agtgaataac tcatacaatc actttaataa tcgtacaata ccattgatga tgatagtaag
      421 tataacgctg ataataatca tggtggtaaa tgggaggtat accgttactc tgcctcgggt
      481 aaatacaaca tcgccaggca aattccccaa aaattttggc gacagttgca atagaatccc
      541 gatgatgatg aaaagtatcc caaacacaat aagcgttctg cccataataa tcccttttat
      601 tagaaaaata gtacagtaaa atcaattcat gccatcattt ggtatgatta atcacatgat
      661 ataataatta ttgacattgt caatgtatta atataaagta tggtatagca tgcatggggt
      721 ttcatgtcat tctgaggtta agctgcctgt gctgatatac gtcaaagtag agaatacaac
      781 cattggacaa atagattctt tggtcactaa tgctccctca gaacgacaaa aattgctgcg
      841 tatcgcaaaa taacaatggt gcatgatttt atgccagggg gaagcgtctc accggttgtc
      901 aacacaaggg gaaaggtagt ttataacagc taacctatga acaacatgga tactatatac
      961 aacagcgatt ttagtctgaa ggagcttatt gacaatctac cggatcttgt attatcagta
     1021 gattatcctt caggtgttat acgctatgcc aacatacgtg caattgatcg gctgggtttt
     1081 tcaataaaaa aggtgcaaaa gctcaaggat ttagcccgtg ttttgttacc tcgctacaga
     1141 aaaaaatatc tacaattctg gcgtgatata aaaggtggaa tacttatacc ctctataacc
     1201 tttgaggtaa aaacacaaga gaatataata atctgggttg aacaaaaaaa tatatatatt
     1261 aaacacaatg acaatgttat ccgtattcat gccgtgatac gtgatataac cgagcggaaa
     1321 aaattag
//
LOCUS       DAOOJH010000231         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_92365_length_1245_cov_6.412605, whole genome shotgun sequence.
ACCESSION   DAOOJH010000231 DAOOJH010000000
VERSION     DAOOJH010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92365_length_1245_cov_6.412605"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLJ75_13400"
     CDS             complement(<1..469)
                     /locus_tag="PLJ75_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPD06251.1"
                     /translation="MIMAIVTVYVVAGKNYLTVNSKTGYQQTTPLKGTIAGIRFSWTV
                     DYIMQEVAYTMDYHTITQYAIALCKQAGAIIVEGYYRDDITIDYKSRTNLVTDIDYES
                     EKYIVSKLKEQFPDFDIVAEEGGLECADGEYIWYVDPLDATNNFAHKIPWFCVT"
     gene            624..>1245
                     /locus_tag="PLJ75_13405"
     CDS             624..>1245
                     /locus_tag="PLJ75_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD06252.1"
                     /translation="MKKTIMVIGGGLLQVPIIQTAQKMGLRVIVTDYNPNALGMKYAD
                     IPIVMSTRDIEGSVRVAKKQNEITPISGVLTAGTDASVTVAAVANALNLPGIKFEDAI
                     AATNKIKMRTRFFEHGVPSPHFFPVWSLSDAKKASKVLGFPVVIKPSDNMGARGVVRI
                     DNKNQVADAFAFAKSASPSGELIIEEFMNGHELSIDAVVYNGEITIT"
BASE COUNT          363 a          269 c          242 g          371 t
ORIGIN      
        1 tggtaacaca aaaccatggg attttatgag caaaattatt tgttgcatcc agagggtcaa
       61 cataccatat atactcacca tcagcacatt ccagaccccc ttcttccgca actatatcaa
      121 agtccgggaa ttgttcttta agcttactta caatatattt ttctgactcg tagtcaatat
      181 ccgtaaccag atttgttctg cttttataat ctatagttat atcatcgcga taataccctt
      241 caacaataat tgcacctgcc tgtttgcata gagcaatggc atattgtgta atggtgtgat
      301 agtccatggt atatgctacc tcctgcatga tataatcaac tgtccatgaa aatcgtatgc
      361 cagctatcgt acctttaagt ggcgttgttt gttgatagcc tgttttacta ttcactgtca
      421 aataattttt acctgctaca acatatacag taactatcgc cataatcatt gtttacttta
      481 cagccggcaa tattaaagta ttggcaacaa ccctcacaat gcaaccaaaa attaccttca
      541 agtttttgct aaaaaaagcc aataataact ctgtagaggc aactcagcag gttgtgactt
      601 tactattgtc cagggggaaa tccatgaaaa aaacgattat ggtgattggt ggcggtttat
      661 tacaggtgcc cattatacag acggctcaaa aaatgggatt gcgggtgata gttactgatt
      721 ataatcccaa tgccctgggt atgaaatatg ccgatattcc tattgttatg agcacccgtg
      781 atattgaagg ttctgtacgt gttgcaaaaa agcaaaacga gataaccccc atatcaggtg
      841 tccttaccgc tggcaccgat gcttccgtta ccgttgcagc agtagcaaac gcccttaacc
      901 ttcccggcat aaaatttgaa gatgccatag cagctaccaa taagataaag atgcgtaccc
      961 gcttctttga gcatggcgtt ccatcacctc actttttccc ggtatggtcg ctttctgatg
     1021 caaaaaaagc aagcaaggta ttgggtttcc ctgttgttat aaagccatcg gataatatgg
     1081 gcgcacgcgg cgttgtgcgc attgacaaca aaaatcaggt tgctgatgcc tttgcttttg
     1141 caaaaagcgc ttcgccaagc ggtgaactaa tcattgagga atttatgaac gggcatgagc
     1201 tttccataga tgccgttgtg tataatggcg agattaccat taccg
//
LOCUS       DAOOJH010000232         1100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_2758
            NODE_112644_length_1100_cov_7.770335, whole genome shotgun
            sequence.
ACCESSION   DAOOJH010000232 DAOOJH010000000
VERSION     DAOOJH010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864833
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:15:33
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,639
            Genes (coding)                    :: 2,622
            CDSs (with protein)               :: 2,622
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 1 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 4 of 17
            Pseudo Genes (multiple problems)  :: 1 of 17
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112644_length_1100_cov_7.770335"
                     /isolate="AMR_MDS_2758"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLJ75_13410"
     CDS             <1..911
                     /locus_tag="PLJ75_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06253.1"
                     /translation="EPLPHTHDYAPIVHAYHEHTGTIAITAERSEEFIDIVPPTHDSA
                     VIYGKNVANKDNAIGVAGIVQSNNGHTIKAYGVLGHASLCGVRGQSQGNSEGIQGCGV
                     MGLSRFGAGGVFVSEHNYSLVADGYGTIAHYDDTVRLVGNGKALYVNGQSYFNGTVSL
                     NSDNKEYYASIVEMFETDDKEYISPGDVLVVSEQGNAILSRSRSAFNKGVIGVVAGNP
                     VMVINNASSEKKIYPVALTGSVLCKVDARQKGVKPGDLLVTSDTPGCAMPALIDSFDK
                     IGTVFAKALSALDDEIALIPVMIWKM"
     gene            complement(913..>1100)
                     /locus_tag="PLJ75_13415"
     CDS             complement(913..>1100)
                     /locus_tag="PLJ75_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD06254.1"
                     /translation="QGAYIWDSESTAVVLWVDSYEKKPFCRKITYISKIMAKDINEYY
                     ATVFSKKEQEIIKKLQP"
BASE COUNT          306 a          190 c          270 g          334 t
ORIGIN      
        1 gcgaaccact gcctcatact catgattacg ctcccatagt acatgcctat catgagcata
       61 caggaactat tgctataact gcagagaggt cagaagaatt tattgatatt gtaccgccaa
      121 ctcatgatag tgcggttatt tacggaaaaa atgtagcaaa taaggataat gccattggcg
      181 ttgcagggat agttcagagc aacaatggac atacaataaa ggcatacggt gtgctgggtc
      241 atgcctcgtt gtgtggtgta cggggacagt cacagggtaa ttcagaagga attcagggat
      301 gtggagtcat gggtttatcg cgatttggtg cgggaggtgt gtttgtaagt gagcataact
      361 attcactggt ggctgatggc tatggaacta tcgcccatta tgatgatact gtacgccttg
      421 ttggtaatgg gaaggcttta tatgttaacg ggcaatcata ttttaacggt acggttagcc
      481 tgaatagcga taataaagaa tattatgcaa gcattgttga gatgtttgaa accgatgata
      541 aggaatatat ttcacctggt gatgttcttg tggtcagtga acaggggaac gcaattcttt
      601 cacgctcacg aagtgcgttt aataaaggtg tgattggtgt tgttgctggc aatccggtta
      661 tggttatcaa taatgccagc tcagaaaaaa agatataccc cgttgcttta acaggctcag
      721 tgttgtgcaa agttgatgca cgtcaaaaag gggtaaaacc cggtgacctt ttagtaactt
      781 cggacacgcc tggatgtgca atgccagcac ttattgattc atttgataag attggaacgg
      841 tgtttgcaaa agcactcagt gcacttgatg atgaaatagc tcttattcct gtgatgatat
      901 ggaagatgta aattatggct gtagcttctt gatgatttcc tgctcctttt tgctgaagac
      961 tgtagcgtaa tattcgttga tatcttttgc cataattttg cttatatagg ttatcttacg
     1021 gcagaacggt tttttttcat agctgtctac ccataaaaca acagccgttg attcagagtc
     1081 ccatatatag gcgccctgat
//
